LOCUS       DBDESG010000001       105008 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.001, whole genome shotgun sequence.
ACCESSION   DBDESG010000001 DBDESG010000000
VERSION     DBDESG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 105008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.001"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..435
                     /locus_tag="ACKB38_000001"
     CDS             <1..435
                     /locus_tag="ACKB38_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978863.1"
                     /translation="SNGAMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGD
                     MATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEAS
                     GAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
     gene            836..1957
                     /gene="mnmA"
                     /locus_tag="ACKB38_000002"
     CDS             836..1957
                     /gene="mnmA"
                     /locus_tag="ACKB38_000002"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGL7978864.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2098..2553
                     /locus_tag="ACKB38_000003"
     CDS             2098..2553
                     /locus_tag="ACKB38_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGL7978865.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPVFLKTALPDWEGQLRHIHLEE"
     gene            2563..4476
                     /gene="mnmG"
                     /locus_tag="ACKB38_000004"
     CDS             2563..4476
                     /gene="mnmG"
                     /locus_tag="ACKB38_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGL7978866.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4608..4757)
                     /locus_tag="ACKB38_000005"
     CDS             complement(4608..4757)
                     /locus_tag="ACKB38_000005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978867.1"
                     /translation="MKIKEQTRKLAVGCSKHCFEVVDRTDEVSSHTYGKATLTRFEEI
                     FEEYQ"
     gene            complement(4829..6508)
                     /locus_tag="ACKB38_000006"
     CDS             complement(4829..6508)
                     /locus_tag="ACKB38_000006"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGL7978868.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6510..6743)
                     /locus_tag="ACKB38_000007"
     CDS             complement(6510..6743)
                     /locus_tag="ACKB38_000007"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGL7978869.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            7023..7238
                     /locus_tag="ACKB38_000008"
     CDS             7023..7238
                     /locus_tag="ACKB38_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978870.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7561..7713)
                     /gene="cibB"
                     /locus_tag="ACKB38_000009"
     CDS             complement(7561..7713)
                     /gene="cibB"
                     /locus_tag="ACKB38_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGL7978871.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7715..7900)
                     /gene="cibA"
                     /locus_tag="ACKB38_000010"
     CDS             complement(7715..7900)
                     /gene="cibA"
                     /locus_tag="ACKB38_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGL7978872.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFRVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            8078..8761
                     /gene="tsaB"
                     /locus_tag="ACKB38_000011"
     CDS             8078..8761
                     /gene="tsaB"
                     /locus_tag="ACKB38_000011"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGL7978873.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8758..9195
                     /gene="rimI"
                     /locus_tag="ACKB38_000012"
     CDS             8758..9195
                     /gene="rimI"
                     /locus_tag="ACKB38_000012"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGL7978874.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMTVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            9185..10195
                     /gene="tsaD"
                     /locus_tag="ACKB38_000013"
     CDS             9185..10195
                     /gene="tsaD"
                     /locus_tag="ACKB38_000013"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGL7978875.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKVFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKILVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10241..11541)
                     /locus_tag="ACKB38_000014"
                     /pseudo
     CDS             complement(10241..11541)
                     /locus_tag="ACKB38_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11877..12568)
                     /locus_tag="ACKB38_000015"
                     /pseudo
     CDS             complement(11877..12568)
                     /locus_tag="ACKB38_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12811..13365
                     /locus_tag="ACKB38_000016"
     CDS             12811..13365
                     /locus_tag="ACKB38_000016"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGL7978876.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKKIGEYNKK"
     gene            13474..14016
                     /locus_tag="ACKB38_000017"
     CDS             13474..14016
                     /locus_tag="ACKB38_000017"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGL7978877.1"
                     /translation="MCYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAAL
                     KEKICHLKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLAS
                     NVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQ
                     KMFVETMRVYDELLEKSSHG"
     gene            14009..14884
                     /locus_tag="ACKB38_000018"
     CDS             14009..14884
                     /locus_tag="ACKB38_000018"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7978878.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVCSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLF"
     gene            14971..16587
                     /locus_tag="ACKB38_000019"
     CDS             14971..16587
                     /locus_tag="ACKB38_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7978879.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16848..17276
                     /locus_tag="ACKB38_000020"
     CDS             16848..17276
                     /locus_tag="ACKB38_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978880.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPGTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            17288..18037
                     /locus_tag="ACKB38_000021"
     CDS             17288..18037
                     /locus_tag="ACKB38_000021"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGL7978881.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVAHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKNYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            18050..19221
                     /locus_tag="ACKB38_000022"
                     /pseudo
     CDS             18050..19221
                     /locus_tag="ACKB38_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            19617..20480
                     /locus_tag="ACKB38_000023"
     CDS             19617..20480
                     /locus_tag="ACKB38_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGL7978882.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLIALFE
                     LRFQQYKELID"
     gene            20766..20999
                     /locus_tag="ACKB38_000024"
     CDS             20766..20999
                     /locus_tag="ACKB38_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978883.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            21021..21698
                     /locus_tag="ACKB38_000025"
     CDS             21021..21698
                     /locus_tag="ACKB38_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGL7978884.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            21710..22375
                     /locus_tag="ACKB38_000026"
     CDS             21710..22375
                     /locus_tag="ACKB38_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGL7978885.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            22447..23673
                     /locus_tag="ACKB38_000027"
     CDS             22447..23673
                     /locus_tag="ACKB38_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGL7978886.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(23908..24147)
                     /locus_tag="ACKB38_000028"
     CDS             complement(23908..24147)
                     /locus_tag="ACKB38_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978887.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFEVVDRTDEVS
                     SKHRFEVVDRTDEVSNHTYGKVKLTWFEESFEEYK"
     gene            24218..24874
                     /locus_tag="ACKB38_000029"
     CDS             24218..24874
                     /locus_tag="ACKB38_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGL7978888.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVMSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            24864..25187
                     /locus_tag="ACKB38_000030"
                     /pseudo
     CDS             24864..25187
                     /locus_tag="ACKB38_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
     gene            25291..26121
                     /locus_tag="ACKB38_000031"
     CDS             25291..26121
                     /locus_tag="ACKB38_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7978889.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            26275..27129
                     /locus_tag="ACKB38_000032"
     CDS             26275..27129
                     /locus_tag="ACKB38_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGL7978890.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKSLFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            27229..28602
                     /locus_tag="ACKB38_000033"
     CDS             27229..28602
                     /locus_tag="ACKB38_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGL7978891.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            28595..29656
                     /locus_tag="ACKB38_000034"
     CDS             28595..29656
                     /locus_tag="ACKB38_000034"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGL7978892.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            29658..30350
                     /locus_tag="ACKB38_000035"
     CDS             29658..30350
                     /locus_tag="ACKB38_000035"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGL7978893.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(30380..30949)
                     /locus_tag="ACKB38_000036"
     CDS             complement(30380..30949)
                     /locus_tag="ACKB38_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGL7978894.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            31084..31698
                     /locus_tag="ACKB38_000037"
     CDS             31084..31698
                     /locus_tag="ACKB38_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGL7978895.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            31676..33346
                     /locus_tag="ACKB38_000038"
     CDS             31676..33346
                     /locus_tag="ACKB38_000038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGL7978896.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HMGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            33358..34644
                     /locus_tag="ACKB38_000039"
     CDS             33358..34644
                     /locus_tag="ACKB38_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGL7978897.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLSEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHFKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            34696..35229
                     /locus_tag="ACKB38_000040"
     CDS             34696..35229
                     /locus_tag="ACKB38_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7978898.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            35307..35777
                     /gene="nrdI"
                     /locus_tag="ACKB38_000041"
     CDS             35307..35777
                     /gene="nrdI"
                     /locus_tag="ACKB38_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGL7978899.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(36070..37332)
                     /locus_tag="ACKB38_000042"
     CDS             complement(36070..37332)
                     /locus_tag="ACKB38_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGL7978900.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(37643..38101)
                     /locus_tag="ACKB38_000043"
     CDS             complement(37643..38101)
                     /locus_tag="ACKB38_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGL7978901.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(38098..38538)
                     /locus_tag="ACKB38_000044"
     CDS             complement(38098..38538)
                     /locus_tag="ACKB38_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGL7978902.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            38971..39837
                     /locus_tag="ACKB38_000045"
     CDS             38971..39837
                     /locus_tag="ACKB38_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7978903.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            39990..40118
                     /locus_tag="ACKB38_000046"
     CDS             39990..40118
                     /locus_tag="ACKB38_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978904.1"
                     /translation="MRSLFKKIVAVLVIGLILLGIARTPQVHKMARGIDPGPANFI"
     gene            40163..40720
                     /locus_tag="ACKB38_000047"
     CDS             40163..40720
                     /locus_tag="ACKB38_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HGL7978905.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            40941..42047
                     /locus_tag="ACKB38_000048"
     CDS             40941..42047
                     /locus_tag="ACKB38_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGL7978906.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            42023..43414
                     /locus_tag="ACKB38_000049"
     CDS             42023..43414
                     /locus_tag="ACKB38_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978907.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            43411..44589
                     /locus_tag="ACKB38_000050"
     CDS             43411..44589
                     /locus_tag="ACKB38_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGL7978908.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            44627..44990
                     /locus_tag="ACKB38_000051"
                     /pseudo
     CDS             44627..44990
                     /locus_tag="ACKB38_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            45014..45247
                     /locus_tag="ACKB38_000052"
     CDS             45014..45247
                     /locus_tag="ACKB38_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGL7978909.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            complement(45213..45344)
                     /locus_tag="ACKB38_000053"
                     /pseudo
     CDS             complement(45213..45344)
                     /locus_tag="ACKB38_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152639374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            45530..47479
                     /gene="mutL"
                     /locus_tag="ACKB38_000054"
     CDS             45530..47479
                     /gene="mutL"
                     /locus_tag="ACKB38_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGL7978910.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(47804..48271)
                     /gene="ribE"
                     /locus_tag="ACKB38_000055"
     CDS             complement(47804..48271)
                     /gene="ribE"
                     /locus_tag="ACKB38_000055"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGL7978911.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(48272..49477)
                     /locus_tag="ACKB38_000056"
     CDS             complement(48272..49477)
                     /locus_tag="ACKB38_000056"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGL7978912.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAKMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(49497..50132)
                     /gene="ribE"
                     /locus_tag="ACKB38_000057"
     CDS             complement(49497..50132)
                     /gene="ribE"
                     /locus_tag="ACKB38_000057"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGL7978913.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(50117..51217)
                     /gene="ribD"
                     /locus_tag="ACKB38_000058"
     CDS             complement(50117..51217)
                     /gene="ribD"
                     /locus_tag="ACKB38_000058"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGL7978914.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKVFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(51351..51465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            51622..52215
                     /gene="ruvA"
                     /locus_tag="ACKB38_000059"
     CDS             51622..52215
                     /gene="ruvA"
                     /locus_tag="ACKB38_000059"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGL7978915.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            52225..52788
                     /locus_tag="ACKB38_000060"
     CDS             52225..52788
                     /locus_tag="ACKB38_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGL7978916.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            52785..53462
                     /locus_tag="ACKB38_000061"
     CDS             52785..53462
                     /locus_tag="ACKB38_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGL7978917.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(53599..54630)
                     /locus_tag="ACKB38_000062"
     CDS             complement(53599..54630)
                     /locus_tag="ACKB38_000062"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGL7978918.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(54885..55568)
                     /locus_tag="ACKB38_000063"
     CDS             complement(54885..55568)
                     /locus_tag="ACKB38_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGL7978919.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(55580..56524)
                     /locus_tag="ACKB38_000064"
     CDS             complement(55580..56524)
                     /locus_tag="ACKB38_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGL7978920.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            56656..59487
                     /gene="uvrA"
                     /locus_tag="ACKB38_000065"
     CDS             56656..59487
                     /gene="uvrA"
                     /locus_tag="ACKB38_000065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGL7978921.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKSISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            59480..60541
                     /locus_tag="ACKB38_000066"
     CDS             59480..60541
                     /locus_tag="ACKB38_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGL7978922.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            60673..61071
                     /gene="spx"
                     /locus_tag="ACKB38_000067"
     CDS             60673..61071
                     /gene="spx"
                     /locus_tag="ACKB38_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGL7978923.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            61137..61706
                     /locus_tag="ACKB38_000068"
     CDS             61137..61706
                     /locus_tag="ACKB38_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGL7978924.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            61793..62059
                     /locus_tag="ACKB38_000069"
     CDS             61793..62059
                     /locus_tag="ACKB38_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGL7978925.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            62063..62482
                     /gene="ruvX"
                     /locus_tag="ACKB38_000070"
     CDS             62063..62482
                     /gene="ruvX"
                     /locus_tag="ACKB38_000070"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGL7978926.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            62498..62803
                     /locus_tag="ACKB38_000071"
     CDS             62498..62803
                     /locus_tag="ACKB38_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGL7978927.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            63062..64312
                     /locus_tag="ACKB38_000072"
     CDS             63062..64312
                     /locus_tag="ACKB38_000072"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGL7978928.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGEFAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLACQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMRERKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLAEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            64396..64854
                     /locus_tag="ACKB38_000073"
     CDS             64396..64854
                     /locus_tag="ACKB38_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGL7978929.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPVAQQQVQQPAQQ
                     NTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESD
                     GDQKVKQVTLDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            65000..66532
                     /gene="cls"
                     /locus_tag="ACKB38_000074"
     CDS             65000..66532
                     /gene="cls"
                     /locus_tag="ACKB38_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGL7978930.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            66610..66753
                     /locus_tag="ACKB38_000075"
     CDS             66610..66753
                     /locus_tag="ACKB38_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978931.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            66790..68328
                     /locus_tag="ACKB38_000076"
     CDS             66790..68328
                     /locus_tag="ACKB38_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGL7978932.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            68448..70655
                     /gene="nrdD"
                     /locus_tag="ACKB38_000077"
     CDS             68448..70655
                     /gene="nrdD"
                     /locus_tag="ACKB38_000077"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGL7978933.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKCLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            70667..70807
                     /locus_tag="ACKB38_000078"
     CDS             70667..70807
                     /locus_tag="ACKB38_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978934.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            70851..71357
                     /locus_tag="ACKB38_000079"
     CDS             70851..71357
                     /locus_tag="ACKB38_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7978935.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVSAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            71350..71940
                     /gene="nrdG"
                     /locus_tag="ACKB38_000080"
     CDS             71350..71940
                     /gene="nrdG"
                     /locus_tag="ACKB38_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGL7978936.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            71937..72554
                     /locus_tag="ACKB38_000081"
     CDS             71937..72554
                     /locus_tag="ACKB38_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGL7978937.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            72821..73129
                     /gene="rpsJ"
                     /locus_tag="ACKB38_000082"
     CDS             72821..73129
                     /gene="rpsJ"
                     /locus_tag="ACKB38_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGL7978938.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            73346..73972
                     /gene="rplC"
                     /locus_tag="ACKB38_000083"
     CDS             73346..73972
                     /gene="rplC"
                     /locus_tag="ACKB38_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGL7978939.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            73997..74620
                     /gene="rplD"
                     /locus_tag="ACKB38_000084"
     CDS             73997..74620
                     /gene="rplD"
                     /locus_tag="ACKB38_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGL7978940.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            74620..74916
                     /locus_tag="ACKB38_000085"
     CDS             74620..74916
                     /locus_tag="ACKB38_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGL7978941.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            74934..75767
                     /gene="rplB"
                     /locus_tag="ACKB38_000086"
     CDS             74934..75767
                     /gene="rplB"
                     /locus_tag="ACKB38_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGL7978942.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            75871..76152
                     /gene="rpsS"
                     /locus_tag="ACKB38_000087"
     CDS             75871..76152
                     /gene="rpsS"
                     /locus_tag="ACKB38_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGL7978943.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            76164..76508
                     /gene="rplV"
                     /locus_tag="ACKB38_000088"
     CDS             76164..76508
                     /gene="rplV"
                     /locus_tag="ACKB38_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGL7978944.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            76521..77174
                     /gene="rpsC"
                     /locus_tag="ACKB38_000089"
     CDS             76521..77174
                     /gene="rpsC"
                     /locus_tag="ACKB38_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGL7978945.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            77178..77591
                     /gene="rplP"
                     /locus_tag="ACKB38_000090"
     CDS             77178..77591
                     /gene="rplP"
                     /locus_tag="ACKB38_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGL7978946.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            77601..77807
                     /gene="rpmC"
                     /locus_tag="ACKB38_000091"
     CDS             77601..77807
                     /gene="rpmC"
                     /locus_tag="ACKB38_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGL7978947.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            77832..78092
                     /gene="rpsQ"
                     /locus_tag="ACKB38_000092"
     CDS             77832..78092
                     /gene="rpsQ"
                     /locus_tag="ACKB38_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGL7978948.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            78118..78486
                     /gene="rplN"
                     /locus_tag="ACKB38_000093"
     CDS             78118..78486
                     /gene="rplN"
                     /locus_tag="ACKB38_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGL7978949.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            78564..78869
                     /gene="rplX"
                     /locus_tag="ACKB38_000094"
     CDS             78564..78869
                     /gene="rplX"
                     /locus_tag="ACKB38_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGL7978950.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            78893..79435
                     /gene="rplE"
                     /locus_tag="ACKB38_000095"
     CDS             78893..79435
                     /gene="rplE"
                     /locus_tag="ACKB38_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGL7978951.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            79453..79722
                     /gene="rpsN"
                     /locus_tag="ACKB38_000096"
     CDS             79453..79722
                     /gene="rpsN"
                     /locus_tag="ACKB38_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGL7978952.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            80007..80405
                     /gene="rpsH"
                     /locus_tag="ACKB38_000097"
     CDS             80007..80405
                     /gene="rpsH"
                     /locus_tag="ACKB38_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGL7978953.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            80597..81133
                     /gene="rplF"
                     /locus_tag="ACKB38_000098"
     CDS             80597..81133
                     /gene="rplF"
                     /locus_tag="ACKB38_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGL7978954.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGSKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            81217..81573
                     /gene="rplR"
                     /locus_tag="ACKB38_000099"
     CDS             81217..81573
                     /gene="rplR"
                     /locus_tag="ACKB38_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGL7978955.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            81591..82085
                     /gene="rpsE"
                     /locus_tag="ACKB38_000100"
     CDS             81591..82085
                     /gene="rpsE"
                     /locus_tag="ACKB38_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGL7978956.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            82099..82281
                     /gene="rpmD"
                     /locus_tag="ACKB38_000101"
     CDS             82099..82281
                     /gene="rpmD"
                     /locus_tag="ACKB38_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGL7978957.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            82426..82866
                     /gene="rplO"
                     /locus_tag="ACKB38_000102"
     CDS             82426..82866
                     /gene="rplO"
                     /locus_tag="ACKB38_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGL7978958.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            82879..84189
                     /gene="secY"
                     /locus_tag="ACKB38_000103"
     CDS             82879..84189
                     /gene="secY"
                     /locus_tag="ACKB38_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGL7978959.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            84340..84978
                     /locus_tag="ACKB38_000104"
     CDS             84340..84978
                     /locus_tag="ACKB38_000104"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGL7978960.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            85095..85313
                     /gene="infA"
                     /locus_tag="ACKB38_000105"
     CDS             85095..85313
                     /gene="infA"
                     /locus_tag="ACKB38_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGL7978961.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            85338..85454
                     /gene="rpmJ"
                     /locus_tag="ACKB38_000106"
     CDS             85338..85454
                     /gene="rpmJ"
                     /locus_tag="ACKB38_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGL7978962.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            85472..85837
                     /gene="rpsM"
                     /locus_tag="ACKB38_000107"
     CDS             85472..85837
                     /gene="rpsM"
                     /locus_tag="ACKB38_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGL7978963.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            85855..86238
                     /gene="rpsK"
                     /locus_tag="ACKB38_000108"
     CDS             85855..86238
                     /gene="rpsK"
                     /locus_tag="ACKB38_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGL7978964.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            86281..87216
                     /locus_tag="ACKB38_000109"
     CDS             86281..87216
                     /locus_tag="ACKB38_000109"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGL7978965.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            87228..87614
                     /gene="rplQ"
                     /locus_tag="ACKB38_000110"
     CDS             87228..87614
                     /gene="rplQ"
                     /locus_tag="ACKB38_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGL7978966.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTESRRGDAAPMAIIELV"
     gene            87879..88145
                     /locus_tag="ACKB38_000111"
     CDS             87879..88145
                     /locus_tag="ACKB38_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGL7978967.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            88155..89492
                     /locus_tag="ACKB38_000112"
     CDS             88155..89492
                     /locus_tag="ACKB38_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGL7978968.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            89736..90428
                     /locus_tag="ACKB38_000113"
     CDS             89736..90428
                     /locus_tag="ACKB38_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGL7978969.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGEIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(90531..92176)
                     /locus_tag="ACKB38_000114"
                     /pseudo
     CDS             complement(90531..92176)
                     /locus_tag="ACKB38_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(92166..93257)
                     /locus_tag="ACKB38_000115"
     CDS             complement(92166..93257)
                     /locus_tag="ACKB38_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7978970.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTIRDSVYLGLNTDYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(93270..94286)
                     /locus_tag="ACKB38_000116"
     CDS             complement(93270..94286)
                     /locus_tag="ACKB38_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGL7978971.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKVVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
     gene            complement(94873..95649)
                     /locus_tag="ACKB38_000117"
     CDS             complement(94873..95649)
                     /locus_tag="ACKB38_000117"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGL7978972.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            95771..96517
                     /locus_tag="ACKB38_000118"
     CDS             95771..96517
                     /locus_tag="ACKB38_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGL7978973.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            96530..97510
                     /locus_tag="ACKB38_000119"
     CDS             96530..97510
                     /locus_tag="ACKB38_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HGL7978974.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            97705..98025
                     /locus_tag="ACKB38_000120"
     CDS             97705..98025
                     /locus_tag="ACKB38_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGL7978975.1"
                     /translation="MEMIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            98071..98379
                     /locus_tag="ACKB38_000121"
     CDS             98071..98379
                     /locus_tag="ACKB38_000121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7978976.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            98396..99694
                     /locus_tag="ACKB38_000122"
     CDS             98396..99694
                     /locus_tag="ACKB38_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGL7978977.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            99845..102283
                     /locus_tag="ACKB38_000123"
     CDS             99845..102283
                     /locus_tag="ACKB38_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGL7978978.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGRFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            102302..102970
                     /locus_tag="ACKB38_000124"
     CDS             102302..102970
                     /locus_tag="ACKB38_000124"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGL7978979.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITAAAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            102988..104076
                     /locus_tag="ACKB38_000125"
     CDS             102988..104076
                     /locus_tag="ACKB38_000125"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGL7978980.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            104531..>105008
                     /locus_tag="ACKB38_000126"
     CDS             104531..>105008
                     /locus_tag="ACKB38_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HGL7978981.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDTSKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWV"
BASE COUNT        32179 a        18379 c        22571 g        31879 t
ORIGIN      
        1 agcaatggcg ctatggcgac aggctggctc caatacaatg gctcatggta ctacctcaac
       61 gctaatggtg atatggcgac aggatggctc caatacaacg gttcatggta ttacctcaac
      121 gctaatggtg atatggcgac aggatgggct aaagtcaacg gttcatggta ctacctaaac
      181 gctaacggtg ctatggctac aggttgggct aaagtcaacg gttcatggta ctacctaaac
      241 gctaacggtt caatggcaac aggttgggtg aaagatggag atacctggta ctatcttgaa
      301 gcatcaggtg ctatgaaagc aagccaatgg ttcaaagtat cagataaatg gtactatgtc
      361 aatggcttag gtgcccttgc agtcaacaca actgtagatg gctataaagt caatgccaat
      421 ggtgaatggg tttaagccga ttaaattaaa tcatgttaag aacatttgac attttaattt
      481 tgaaacaaag ataaggttcg attgaataga tttatgttcg tattctttag gtacctatct
      541 tatgatttca ggaaatgtca ttaaaaaaac gactcatttt ctctaacttg aaaaatagat
      601 tagagaaaat gggttgtttt atttattata gttatttgaa tgaagataag aagaaggtat
      661 actcacatca ttcacataat ctgtatattg actataagtt ttaaaaaaca atttttaagc
      721 tcttccttgt cttctctaac caagcgtgtt ataatgaata ctgctcaagc gaccttcaat
      781 cgtgaagcac acacgacctt caatcgtgaa taaacgaata gatgggagac ttaccatgag
      841 tgataactct aaaacacgtg ttgtcgtggg gatgagtggt ggtgttgatt cgtcggtgac
      901 ggctcttttg ctcaaggagc agggctacga tgtgatcggt atcttcatga agaactggga
      961 tgacacagat gaaaacggcg tctgtacggc gaccgaagat tacaaggatg tggttgcggt
     1021 ggcagaccag attggcattc cctactactc tgtcaatttt gaaaaagagt actgggaccg
     1081 cgtttttgag tatttcctag cggaataccg tgcagggcgc acgccaaatc cggacgttat
     1141 gtgcaacaag gaaatcaagt tcaaggcctt tttggactat gccataacct tgggggcaga
     1201 ctatgtagcg actgggcatt atgctcgagt ggcgcgtgat gaggatggta ccgttcacat
     1261 gcttcgtggc gtggacaatg gcaaggatca gacctatttc ctcagccaac tttcgcaaga
     1321 acaacttcaa aaaaccatgt tcccactagg acatttggaa aagcctgaag tacgcagact
     1381 agcagaagaa gcaggccttt cgactgctaa gaagaaagac tcgacaggga tttgctttat
     1441 cggagaaaag aactttaaaa actttctcag caactacctg ccagctcagc ctggtcgcat
     1501 gatgactgtg gatggtcgcg atatgggcga gcatgcaggt cttatgtact atacaatcgg
     1561 tcagcgtggc ggactcggta tcggtgggca acacggcggt gacaatgccc cttggttcgt
     1621 tgtcggaaaa gatctaagca agaatattct ctatgtagga caaggattct accatgattc
     1681 gctcatgtca actagcctag aagccagtca agtccacttt actcgtgaaa tgccagaaga
     1741 gtttacgcta gaatgtacgg ctaaattccg ttaccgtcag cctgactcta aggtgaccgt
     1801 tcatgtcaaa ggagaaaaga cagaggtcat ctttgcggaa ccacaacgcg cgattacacc
     1861 aggacaggca gttgtctttt acgatggcga agagtgtcta ggtggcggtt tgattgacaa
     1921 tgcttaccgc gatggacaag tttgtcagta catttagatt gacaaatttt ctcaatttgc
     1981 tacaataata acagcaatag aaatgatggt caaagctcat ggatgttgca ggcttttttg
     2041 tcctgcactt ctttgtagtt ttgactgttt ttgtgtcgtt taagggaaag gacaagaatg
     2101 actcaacaag actttcggac aaaagtagac aatacggttt ttggtgttcg ggctacagcc
     2161 ttgattgtcc aaaatcacaa gcttctagtt accaaagaca agggcaagta ttacactatt
     2221 ggaggtgcga ttcaagtcaa tgaaagcacg gaagacgcgg tagtccgtga agtgaaggaa
     2281 gaactgggtg tcaaagctca agctgggcag ctagcttttg tggttgaaaa tcgttttgaa
     2341 gtggacggcg tttcctatca caatatcgag tttcattatc tggtggattt gcttgaagat
     2401 gccccgttga ccatgcagga agatgagaaa aggcagccct gtgagtggat tgacttggat
     2461 aagctccaga atatccagct agttccagtc tttttaaaaa cagccctacc agattgggaa
     2521 ggccaactaa gacacattca tcttgaggaa taggagagaa acatgactta tcattttact
     2581 gaagaatacg atattattgt aattggtgcg ggacacgctg gggttgaggc ttccttggcc
     2641 gctagccgta tgggctgtaa ggtcctgctt gcgaccatca atattgaaat gctggctttc
     2701 atgccttgta atccctctat cggtggttct gccaagggga ttgtcgtgcg tgaagtcgat
     2761 gccctcggtg gcgagatggc caaaaccatt gacaagactt acatccagat gaagatgcta
     2821 aacacaggga aggggccagc tgtccgtgcc cttcgtgcgc aggctgacaa ggaactttac
     2881 tctaaggaga tgcgcaagac ggttgaaaac caagaaaatc tgacccttcg tcaaaccatg
     2941 attgatgaga ttttggtgga agatggcaag gttgtcggtg tgcgtacagc cacccatcaa
     3001 gaatatgctg ctaaggctgt tattgtgacg acagggactg ctctccgtgg ggaaattatc
     3061 atcggagacc tcaagtactc atcaggtcct aaccacagct tggcttctat taacctagct
     3121 gacaatctca aggaactggg tctcgaaatc ggtcgtttca agacaggaac ccctccacgt
     3181 gtcaaggctt cttctatcaa ttacgatgtg acagaaattc agccaggaga cgaagtgcct
     3241 aatcatttct catacacttc acgtgatgag gattatgtca aggaccaagt accatgctgg
     3301 ttgacctata ccaatggtac cagtcatgag attatccaaa acaacctcca ccgtgcgcct
     3361 atgtttacag gtgtggtcaa gggagtgggg cctcgttact gtccgtcgat tgaagacaag
     3421 attgtgcgct ttgcggacaa ggaacgtcac caactcttcc ttgagccaga agggcgcaat
     3481 actgaggaag tctatgtgca aggactttca accagtctgc ctgaggatgt ccagcgtgac
     3541 ttggtgcatt ccatcaaagg tttggaaaat gcagagatga tgcggacagg ttatgctatt
     3601 gagtatgata tggtcttgcc tcatcagttg cgtgcgactt tggaaaccaa gaaaatctca
     3661 ggtctcttca ctgctggtca gacaaatgga acatcaggtt acgaagaggc agcaggccaa
     3721 gggattatcg cgggtatcaa tgcggctctg aaaatccaag gcaagcctga attgattttg
     3781 aagcgcagtg atggttatat cggggtgatg atcgacgact tggtgaccaa gggaaccatt
     3841 gaaccctacc gtctcttgac cagtcgtgct gaataccgtc tcattcttcg tcatgacaat
     3901 gctgatatgc gcttgactga gatgggacgc gagattggcc ttgtggacga tgaacgctgg
     3961 gctcgttttg aaatcaagaa aaatcaattt gataatgaga tgaagcgcct agacagtatc
     4021 aaactcaagc cagtcaagga aaccaatgcc aaggttgagg agatgggctt caaacccttg
     4081 accgatgcag tgacagccaa ggaattcctt cgccgtccag aagtttctta ccaagatgtg
     4141 gtggccttca tcggaccagc tgcagaagac ttggatgaca agattatcga attgattgaa
     4201 acagaaatca agtatgaagg ctatatttcc aaagccatgg accaggttgc caagatgaaa
     4261 cgcatggaag aaaaacgcat tccggccaat atcgactggg atgacattga ttctatcgca
     4321 accgaagccc gtcagaagtt caaactcatc aatccagaaa ccatcggcca agccagccgt
     4381 atttcgggag taaacccagc agatatttct attttgatgg tgtatctgga aggtaaaaat
     4441 cgtagtattt ctaaaactct tcaaaaatca aaatgatacg tcgtcggctt cttacgaatg
     4501 agttcaaagc ttggctttga ttcatctcca gcctcccata gtttcccgaa ctatgggagc
     4561 taactcaagt tatgctttcg cttagccttc tagtctgatt tcgattttta ttgatactct
     4621 tcgaaaatct cttcaaaccg cgtcaacgtc gccttgccgt aggtatggct actgacttcg
     4681 tcagttctat ctacaacctc aaagcagtgc tttgagcaac ctacggctag cttcctagtt
     4741 tgctctttga ttttcattga gtatgagttt taatctcaaa agaaagcata gagaaaaaca
     4801 gctccgagga cggggctgtt ttcttgcttt attcttcatc tggtgtgagg atcatcggga
     4861 tgatgatcgg ttcacgttcg gtattttcat agaggaaggg gcgaatagcg ttgacaatgg
     4921 caccattgac agattgcacg ctagcatcct tatttttcag tgcgatacga atggcattga
     4981 agaggatacg ctggctttgg cgaatcaagt cgccagactc tctcatgtag acaaagcctc
     5041 ggctgaggat gtctggacca gatagaatca tctgcgattt gaagtcaaca gttgcaactg
     5101 ccagaacgac accgtcttca gatagatcgc gacgatcttt gaggacagct gcgccaattt
     5161 caccgatacg atttccatcg acatagatat cttgggcgtt gaaatgacct gcgatacgag
     5221 ctgagtcagc agtaagggca agcacatcgc cattgctcat gataaagata ttgtccttct
     5281 caacaccagt atccactgct agtccagcgt ggactttttg catgcggtat tcaccgtgga
     5341 caggcatgaa gtattttggc ttaatcaagc agagcatgag tttttgctct tgctgaccac
     5401 cgtgtccaga tgtatggata ttgttcactt taccgtggat aacttcgaca ccagcttcag
     5461 aaatgatgtt aatcagcttg ttgacactag tagtgtttcc agggatggga ctagaagaga
     5521 agataacggt atcacctggt tgtaattgta cttgacggtg ggttccgttg gcgatacgag
     5581 agagggctgc cataggctca ccctgactac ctgtacagag gataagaact tctcctgcag
     5641 gataatcttt gatttcattt ggctcgataa aggttccctt aggagctttg atgtagccaa
     5701 gatcgattcc gttgacaatg gccttttcca tagaacgacc aaagaccgca atcttgcgtc
     5761 cagtcttaac agcagcttct gttgcctgct ggagacggaa gatatttgag gcaaaggatg
     5821 caaagatgat acgtccttca ataccttgga taatcttcat aatggactga ccaacgactt
     5881 tttcagagtt ggtaaaggtt ggtacttccg catttgtcga gtcagacagg agacagagca
     5941 cgccttcttc accaagcgca gccatacgat gcaagtccgc aggttctcca actggagtaa
     6001 agtcgaactt aaagtcaccc gtacagacga ttttcccttg aggagtatga atgacaatcc
     6061 ccaaaggctc tggaatagag tgagtcgttc taaagaaagt tgccttgaga tttttaaagg
     6121 tcaactcggt gttgtggttg atttcgtaaa gtttggcgtt gcgcaagagg ccgtgttctt
     6181 cgagtttccc acggatcaaa gccaaggcaa gcggtccagc ataaataggg acatttgctt
     6241 gcttgagtag gaacggaatc ccaccaatgt ggtcctcgtg tccgtgtgtg attaaaacag
     6301 ccttgacgcg gtcgatattg tccacgatgt aagagtagtc aggaatgaca tagtcgatac
     6361 caagcaagtc atcttctggg aatttaatcc cagcatcgac gatgataatc tcgtcttggt
     6421 attcaattcc gtaagtgttt ttcccgattt ctcctagacc accgatggca aaaacgccga
     6481 cttcttcagg tttaagagtg taggccatat tagaactccg taatttcgaa ggcgccagtt
     6541 tctttttcgt aatcgagcaa tttgtcagac aagagttcga tatactcgat attgtactct
     6601 gggcgatttt cttcgacaag ttggcgagca gtgatacggc cctcaagttc tgagctggca
     6661 tcgatgtcta ggtaaagcgt gcgtgttgtt tcacggcgtg ggctacgttc ttttgtttct
     6721 tgataaaaaa ctttgtaaat catatagttc ctttctattt acaggtcagc ttattccaaa
     6781 cttttagcag atatttgctt gatttcattc cattttgtgt ctagattgac cttgattcgt
     6841 tacaattatt tcaattatac cacaaaatga aaaattagta aaagacggaa acgagaaagt
     6901 taggggacga tttctttggt aagcgttttc aaaaataaag aaaatgtatt atgttgataa
     6961 cggaagtgaa atttattgct tgtcataatt ccatatagat tttaagtcaa ggaggttgcc
     7021 ctatgtataa cttatttgct tttgctatcg gagctcgctt ggtagctttt attgtttttg
     7081 ttgcttgcgt ttatgcgggg aatcttctat ttgccaaact ggaacgacga caaaccaagt
     7141 tcttgtggaa aatgaccttt gtgactctct actcgctttt tctcctcctc gtgacctatg
     7201 tcgtttggtt tgtattttac tttgaattaa atgcttagat gccctgctta tgctggggtt
     7261 ttttgtttgg gaatgtagag atagtagatt tacgacacga aaattccacc ccatctatca
     7321 agatgaggtg gtctttgcat ttttctaaca tgacatgaca atggaatttt caaagcttat
     7381 tatttttatg ggagtcattg ttctgatcaa aaatagagtt gaagcataaa aataaaacaa
     7441 tacaaacaga taaatcgaat ggtaaaggga tggtagtaat gttcaaatca ttgagaatgc
     7501 tgagcaaact gtaagagatg aagacaataa aatttatcct tctaaaaatg tgtttcttat
     7561 ctagcatgac ttaccaaact ttttacgaag gtcttttcca atggtataac ctgccataaa
     7621 taaagcgata ccaacaccga caccaaagcc acccttgatt tcttgcaatt ccttattaga
     7681 aatttcacga taattgttca aatctttcat catattaatc tcctcctaaa aaataaccag
     7741 ctgccaaacc agaacctata agtgctgttc cacctgcaat agccttccaa gatactgcta
     7801 ctctaaagat aaccaaggga gcgagaccgc catcaatatc tgataattca ttttcagata
     7861 aggataaaaa ttgattgtca agaatgtcaa aatttgtcat tgaactttcc tccttttttc
     7921 ttgttcactt ttatattcgg aaaagaaaag gaaaagtgag cattttttta aattattttt
     7981 agttaagtga gggcactctt gtctgggggc aaagaaaatt cccatgtgcc agcttttgta
     8041 gtataatagt aagcagacaa aaagatagga atgtgttatg aaagtattag cttttgatac
     8101 gtccagcaag gctctttctc tggctatttt agaggataag caggttcttg ccgagacgac
     8161 gattaatatt aagaaaaatc acagtattac tcttatgcct gccatcgatt ttttgatggc
     8221 aagtttggat tggacaccca aggatttgga ccgaatcgtg gtagctgaag ggccgggtag
     8281 ctatacaggc ttgcgaattg cggtagcaac tgctaagacc ttagctcaca ccctgaacat
     8341 cgagttggtt ggtatgtcga gtctcttggc tctggtgccc catcaacaag aaggtttgtt
     8401 tgtccccttg atggatgcgc gtcgcaataa tgtttatgca ggattttatg aaaatgccaa
     8461 acctgtcatg gcagaagcgc acctatcttt tgaagaggtg ctagaaaaag tcaagggtac
     8521 tagtcaggta acctttgtcg gagaagttgg cccctttgtt gagcagattc aaaaacactt
     8581 gccaaggact gattacaaag aaacattgcc caatgcagct aatctagctc ttttggcctg
     8641 ggacaaggaa gcagactcct tgcatgattt tgtgccgaat tacctcaaac gagtcgaggc
     8701 tgaggaaaac tggctcaaga accataccga gtctggcgag tcttacatta aacgcctatg
     8761 atagaaatca agcgaattca acaacagcct gacctagctc aagccatcta cgctgttatg
     8821 gcagctgttt acctagtcag tccttggact ctggagcaaa tccaagcaga tctgtcccaa
     8881 gaccagactt ggtatgcatt ggcttatgat ggggcagaag tgattggatt tctagctgtg
     8941 caggagaatc tttttgaagc agaagtcctg caaatcgctg tcaaaggagc ttatcagggt
     9001 caggggattg cgtcagcctt gtttgctcaa ttgccgacag acaaggaaat tttcctcgaa
     9061 gtcagacagt caaatcaacg agcgcaagca ttttacaaga aagaaaagat gacagttatc
     9121 gctgagcgaa aggcctacta ccatgaccca gtcgaggacg ccattatcat gaagagagaa
     9181 atagatgaag gatagatata ttttagcatt tgagacatcc tgtgatgaga ccagtgtcgc
     9241 cgtcttgaaa aacgacgatg agctcttgtc caatgtcatt gctagtcaaa ttgagagtca
     9301 caaacgtttt ggtggcgtag tgcccgaagt agccagtcgt caccatgtcg aggtcattac
     9361 agcctgtatc gaggaggcat tggcagaagc agggattacc gaagaggacg tgacagctgt
     9421 tgcggttacc tacggaccag gcttggtcgg agccttgcta gttggtttgt cagctgccaa
     9481 ggtctttgct tgggctcacg gacttccact gattcctgtt aatcacatgg ctgggcacct
     9541 catggcagct cagagtgtgg agcctttgga gtttcccttg ctagccctct tggtcagcgg
     9601 cggacacaca gagttggttt atgtttcgga ggcaggagat tataagattg ttggggaaac
     9661 ccgtgatgat gcggttggtg aggcttatga taaggtcggc cgtgtcatgg gcttgaccta
     9721 tcctgcaggt cgtgagattg acgagctggc tcatcagggg caggatattt atgatttccc
     9781 ccgtgccatg attaaggaag ataatctgga gttctccttc tcaggtttga aatctgcctt
     9841 tatcaatctt catcacaatg ccgagcaaaa gggagaaagc ctgtctacag aagatttgtg
     9901 tgcttccttc caagcagcag ttatggacat tctcatggca aaaaccaaga aggctttgga
     9961 gaaatatcct gttaaaatcc tagttgtggc aggtggtgtg gcagccaata aaggtctcag
    10021 agaacgccta gcagccgaaa tcacagatgt caaggttatc atcccccctc tgcgactctg
    10081 cggagacaat gcaggtatga ttgcctatgc cagcgtcagc gagtggaaca aagaaaactt
    10141 cgcaggctgg gacctcaatg ccaaaccaag tcttgccttt gataccatgg aataaagagt
    10201 ggctctttgt caagtgtagt gggtaggcga aaagctgcaa tctggagagt acgaaattcg
    10261 tactctcttt tttgatgttc aaagtcatca aaatccgttt tttaaaattg ttaaagttcc
    10321 ggaaaccaaa ggctttgcgc ttgataatct taatcaaatt attggtcgct tcaagtttag
    10381 cgttggaata aggcagctta agtgcgttga taatcttatc tctatccctt aaaaaagtcc
    10441 aaaagaccgt ttgaaaaagc ggatgaaccg ttggcagttc ttgctcaatc aatccaaaga
    10501 aatggtcggc attcttctct tgaaaatgaa agagcaggag ttgatagagt tcgtagtgaa
    10561 cctgtaatcc ctcagagtaa gaaagcagct tatctcgtac ttcccgatgg gttaagtgca
    10621 ttcgaaacgt gggctgatag aatcgtttct gacagagctt gaagctatac tggtgtagta
    10681 aatcagtcca gtggactgat ttagcccgag cctagaaacg aaaaagcgag ggttccaaaa
    10741 gcgcttgagc gcccgatact ccaaggattt tcggtcaaat tggttcatga tttggattcg
    10801 tactcggttc atagctcggc tcagatgttg gacaatgtgg aagcggtcaa gaacaatctt
    10861 cgcctttgga aagagttgct tagcgagccg ataataaggg ctgtacatgt ccatggtgat
    10921 gacctcgacc agctcccgaa cctctctctg atagcgttgg aaatggtttc gaatcaccgc
    10981 atgagttcgc ccgtctaaaa ttgcgaggat ggatttggac tcaaaatctt gggcaatgaa
    11041 gctcattttg ctctttttga agctatactc atcccaactc ataacttttg gcaacttggt
    11101 ccaatcggtt tcaaacttaa attccctcag ttttcggatg acggtggaag ttgagaccgc
    11161 caatctgtgg gcaatctcag tcatagactg cttttcaagg aggagctgag cgattttttg
    11221 tctcaccatg ttggaaatct ggcaattttt cttgacaatg gatgtttgag aaacgaccac
    11281 cttaaggcaa ttcttgcact gaaagcggcg ctttttgaga tgcagtaccg tgggtaaacc
    11341 ctgacagtcg agaagcggaa ttttagaggc tttctggaag tcgtatttga tcatcttccc
    11401 ttgacaatga ggacaaggag gagcggggga atccaacttt gcctgaacga ctagatgggt
    11461 ttggtatttc agaacagaca agattttgat atttttgtct tccaatccga gcaaatctgt
    11521 ggtattctta agttgttcca tatgaaccct ttctaatgag ttggttagac gcttttcatt
    11581 ataggtcata tggggctttt tttctacaag aaacgaccct ataattcctg gggtgggatt
    11641 acccactaca gaaattatag agccaaaaca ttccaaagtc ttgtctgact ctggaatgtt
    11701 ttctgttcac agttgttttg taactgccta ataagtaacc gcgctataac gaggtatcta
    11761 ccgagattgt caattctcca gtatactgtt ataaaaaaca aaactggagg atttttttat
    11821 gtccccccat cgtcccatcg actgttccgt agctgcccaa taacggagta tagtcggctg
    11881 aaacaagaat aggacaaaag tgcctcaaga aaagcatcgt aatttgacaa tattattctg
    11941 agatgttttt gatgtaagcc catattttct caatgggatt atactcgggt gaataggtag
    12001 gaagtggtaa cagtctatgc cctgctcctt acacatgttc attctgtgaa accttgcatt
    12061 gtccataatg ataagggatg gtgtatctaa agtaggtagt aagaattttt ggaagcaagc
    12121 ttcgaaaaag tcactcgtca tcgtatcttt ataggtcatc ggagccatag tatattgaaa
    12181 caaaaaaggg cattagtact tctataacag tatcgcaatc tctggtgtgc gttgtctcga
    12241 tcttaattca aataattata tcttcttcca gagagactaa ctgcctgctc aaggctcgat
    12301 catattcgag ataaaaatag gtctcaaccc ctgtctcata aatataaata ggagtcaggt
    12361 agcttaagtg attcaattct ttaaggaacc gatttacttt ttctgggtct tgttcgcagt
    12421 aggtacggct cttttttaga ctatatccca tagcctttag agcgtaatga atagttgttg
    12481 gaggacagtt gaattcagca gctatttcag tcaaataagc atcgggatga gtttcaatat
    12541 agatttttaa tttatctcta tcaatttttt caattaatag tggaagtttt cttgaagtta
    12601 gtgtattgaa acgtttttat acagtgctaa aatttgaagt gtagtgcaga tggatttgta
    12661 atagtattat agcaattctt tggagaaggt cgagacaaga taaaataggt atctttgaga
    12721 tataagcatg atttactata aaattattga gttattcata aaaatttctc tatcaaatat
    12781 cataatgcgt cgtttaagga gatggttaca atgcaatata gttgtggaaa aataaatatt
    12841 aatatccctg atggatatgg agacattaag gatatagtgt tttcggctca tattatagtt
    12901 aggtataata atggacattg tggaggtata gatccgcata taattggact ttgtaaaaag
    12961 cagatcagaa ggatgtctct atatcctatt ttgataattg tatctagaga ttcaaaggtt
    13021 attgatgatt ataaaaattt agatattgcc tatgttgatt gtactcaatg ttcaaataat
    13081 tttgaaactg ccttacacgt taaaaatatt ttaaaattat taaaaatcca acttatacac
    13141 tgtcatggct actcaactaa ttatttttta tatatgttaa aaaaacttga caagaatgga
    13201 tttggaaagg ttaagacagt gataacatgc catggatggg ttgaatataa cttaaaaaag
    13261 aaattcttga cttattttga tttttggaca tattctatgg gagatgcttt tatttgtgta
    13321 tctgaaacta tgaaaaaaaa gattggagag tataataaaa aataagaaga tagttgcaat
    13381 aaataacgga attaatgtat caaatagcga tttagatgta gtaggtgttc aagactttaa
    13441 aaaagaattt tgtattccaa ataacaaaaa atcatttgtt atgttggaag gttggatcca
    13501 gaaaaaaggc cggatagatt ccttgaattt gcagaaaaat tatttttagt cagagaagat
    13561 gtgattttta ttatggctgg aaatggtagt atgtgggctg ctttgaaaga aaaaatttgc
    13621 catttgaaat gtagagataa tttcaggtta cttggtgaaa tctatccagc tactatagtt
    13681 taccaaatat cggatttatt gtatattcct tctgatacag aaggaatacc tatgtgtgtt
    13741 ttagagtcaa tgtcacaagg aactccggtt ttagctagta atgttggcgg gttaagtgaa
    13801 attattgaac atagggttga tggattttta tttgagaagg aagatgttga gggagtgtgt
    13861 gcttgtgcta attttttact caatgattct gagtatttga aatatatagg tgagaatagt
    13921 aaatcaaaaa taagaaaaca tttttctgtg caaaaaatgt ttgtagaaac catgagagta
    13981 tatgatgaat tattagagaa gagtagtcat ggataggaat atagatcaag aattagtaag
    14041 tattataata ccaacacata ataggtatga atctctgatt agagctgtga aaagttgttt
    14101 acatcaaagt tataaaaata tcgaagttat aattattgat gataattata gtaatgtaaa
    14161 tttaagaaat aaaattatcc atcaatttgg ctataccaat catagaatta agttaatttt
    14221 aagtaatgaa gatttaggtg caactaatgc cagaaacata ggtatcaaaa attctagagg
    14281 taagtatata tcatttttag acgatgatga tgaatatatg ccagatcgaa ttttaaagtt
    14341 gatggcttgt tttaaaaaga gtagaatgaa gaatttagct ttagtttgta gttatggaat
    14401 aataatttat ccaaatggta cacgagaaga ggagaagacc gattttgttg gaaatccctt
    14461 gtttgttcaa atggttcaca atatagcagg tacgtcattt tggttgtgta aaaaagaggt
    14521 gctagaatta attaatggtt ttgagaaaat agattcacat caggacggtg ttgttttatt
    14581 aaaactactt gttcaaggat accaaattga tatagtgcga gaattcttgg tgaattacta
    14641 cgctcacagt aaagaaaacg gtatcactgg agtgacacaa aaaacaatta atgcagatga
    14701 agaatattat aattactgta ggaaatattt taatttattg agtttcaacg agagaatatt
    14761 ggttacaaag aaatattatt ctttaaacat aaagcggtta ctattaatag gagacaaatg
    14821 caaggcttta aaagtaatca agaaggcaag agaagaaaaa atttttaacg aatttctttt
    14881 ttgaaatata tgttattata tcattttggt tttttctatt gtatatatga aaactatgtt
    14941 caattaaaat ttagaaagtg agaaactatt gtgtatacta ttataaaatc aaatataaaa
    15001 aaatttagtt tattaacgat atttattgtt gctggtcaat tattgctaat ttatgcagca
    15061 actattaatg ctctggtgtt gaatgaatta attgcgatga atttagagcg gtttttgaaa
    15121 ttgtcaatct accaaatgat tgtctggtgt gggataatat tccttgactg ggtagtgaaa
    15181 aattatcagg ttgaagtgat ccaagagttt aatctagaga ttcgaaatag agttgccaca
    15241 gacatctcta actctaccta tcaagaattt catagtaaat catcaggaac atatctttcg
    15301 tggctaaata atgatgttca gactttaaat gatcaggcgt ttaaacaact ttttttagta
    15361 ataaaaggaa tttctggtac tatatttgca gttgtgactc ttaatcacta tcattggtca
    15421 ttgactgtag ccaccttgtt ttcattaatg attatgctac ttgtaccaaa aatctttgca
    15481 tcgaaaatgc gagaagttag tctaaattta actaaccaaa atgaagcttt tttaaaatct
    15541 agtgagacta tattgaatgg atttgatgtg ttagcgtcct tgaatctttt atatgtattg
    15601 cctaagaaaa ttaaagaagc aggaatttta ttaaagatgg ttatacaaag aaagacaact
    15661 gtagaaacgt tagcaggcgc tattagcttc tttctcaata ttttttttca gatatctctc
    15721 gtttttttaa caggctatct tgcaataaaa ggaatagtga aaattggtac tattgaagca
    15781 ataggagcac taacaggtgt tatttttaca gcgctaggtg aattaggagg tcaattatcc
    15841 tctattattg gtacgaagcc tattttttta aaattgtatt caattaatcc aattgagtca
    15901 aataaaatga atgatatcga accaaatgag gtgaatagag attttccgtt atatgaagca
    15961 aaaaatattt gctataagta tggagataaa gaaatattaa aaaacttaaa tttttgtttt
    16021 caacgtaatg aaaagtattt aattttaggt gaaagtggaa gcgggaaatc tacattatta
    16081 aaattattga atggcttttt gagagattat agtggagaat tgcgattctg cggggatgat
    16141 ataaaaaaaa cctcctattt aaatatggtt tcgaatgttc tatatgtaga tcaaaaagct
    16201 tatttgtttg aaggtacgat tagagataat attttattgg aagaaaatta tactgatgaa
    16261 gaaatactac agtctttaga gcaagttggt ttgagtgtaa aagattttcc taataacatt
    16321 ttagattatt atgttggtga tgatgggaga ttactgtcag gagggcagaa acaaaaaatt
    16381 actttagcta gagggctaat tagaaataag aaaatagtat taattgacga gggaacttct
    16441 gctatcgata ggagaacttc gttagcgatt gaacgtaaga tattagatag agaggatttg
    16501 actgtcatta ttgttaccca tgctccgcat ccggaactta aacaatattt tactaagata
    16561 tatcaatttc caaaggattt tatttaagaa tataggggag ggaaacatga gattctattg
    16621 tgaagcttat gaggtaatta ctgaagagaa aaaagtatca tttttttatg tagatgagaa
    16681 acccttaagg tatctgttat ttgactatat atttctacta gttctagcta aaaaaaaata
    16741 tcccaatctt atgaactgca ccccaaaagt tagatttatt ctgtctaact tttggggtgc
    16801 agttcatttt gcatagtttt ggcattcatc ctagatatcc atataaaatg tctgagattg
    16861 ttgaaatgtc taaaaaaata tcagatctaa acgataaaaa agaatttcac aaaaagaact
    16921 gtgagttgag gtttcaagat agtgttcttg ataacataaa tagtgtcccg ggtacaactc
    16981 atttatttag ttgtttaaat attcaaacat tagtaaaaat tattttatat atttttcgat
    17041 acaacagttg tatgaaaata tttaatttta gagataaaga gcttatcttt tttaataaga
    17101 ttgtaaatgg gctgatacaa aatattgagg agaatttaga ggatgatatt gaaaggatat
    17161 tgaaatattt atatatatgt ttatttaacg aaatattcat tattaagaat aaggtgaatt
    17221 tttttgatga tgtggaattt aatcagacac ttagtgagtt tttagataaa ttgtagaggg
    17281 aatggttatg agtaacaata ctaagtatat ctttttatct ccacatttag atgacgcaat
    17341 tttttcctgt ggtgattata tttcaaaatt aacttcagaa ggagaaattg tacttgttat
    17401 tactatattt tctggttacc ctttgagcca acagctacag ccctctgcta agcaatttca
    17461 taagttgtgt aaccttggga aatatccgat tgaagaaagg aagaaagaag acaggttagc
    17521 ttgtgaacgt ctacaatgtg attttagaca cttatcgtac tatgaatgtc tctataggaa
    17581 agatagaaat ggcaattttc tataccgtca tatctatagt gaattaaaaa atgaggacac
    17641 attaaagaat gatatcataa aagagctgtt aatgcattta gacgacaaat gtgtcgtata
    17701 ttgtccgctt tcacttggtg accatgttgc tcatgttttt gtgaatagta ttggaagggc
    17761 gcttgaattt atgcgttata aagtaatata ttatgaggac ttcccatatg taagtgatag
    17821 tagtatggtt agttatatgg gaaaaactaa agaattaaaa atgtatcaag aggaacttga
    17881 tgaaaaaaat tatattgata gaatatcatc aatattgtgc tataaatctc agattttgat
    17941 aatctggaaa tcagttgaaa aacttttaaa caatattaaa gagttgtatt taaggaatgg
    18001 ggcagcttat tctattagat tttggattaa gaaataagga agtagtttta tgaatatagc
    18061 agtaattggt ttggggcatg ttgggctggc ctacgcgtta ctatttgcat ctaaatataa
    18121 agttgttgca tatgatatag attctgtaaa aataaataat ttaaaaaagg gcattcttcc
    18181 atctaaaaat gaagagctta tgaagttttt ttgcgagaat aacttaaata ttactttttt
    18241 tgatacattt tctgaaatta aaaataatat tgattattat attattgcgc ttccgacaga
    18301 ttatgatgag aaaattggta gttttaatac atatgaaatc gaacaaacgg tatcgaagat
    18361 tctgagggta aaacctaatg gaaagattat tttaaagtca acagttccga tcggcttttc
    18421 aaacaaatta aaaaggctgt ttgatacaaa aaatatcatt tttgtccctg aatttttgag
    18481 agaaggttgt tctatatatg ataatttata tccaagtcgc atagttgttg gagatgagac
    18541 agttgaagga agaaaaattg cagagttgtt cctttcgatt agtactcata gtactgccaa
    18601 tattaaaaat gttatgttag tttctcctac tgaagcagaa gcaattaagc ttttttctaa
    18661 cacattctta gctctccgtg ttgctttttt aatgaactag attcttttgc tgagaggaga
    18721 agcttaaatg ctgaagttgt aataaaaggt gtttgtttag atccaagaat tggaaatttt
    18781 tataataatc cttcttttgg atttggagga tattgtcttc ccaaagatac taaacaatta
    18841 aaaaaagaat ttatagaaat aaatgcccca gtgatagaag cgattgatat ttcaaacaca
    18901 aatagaaaac agttcatagt taaacaaata ttggaacgaa agccaaagat agtaggaata
    18961 tataaattgg ggatgaaata taattcagat aattacaaag agtcagctat tttaagtata
    19021 attaatgaac tattgattgt cggtataaaa attttagtat atgaaccaaa tttaaacgtt
    19081 agtatagata acgtaattat tgaaaagaat tttgaactat ttacaaaaca aagtgatcta
    19141 attgttgcaa atagatggga taggggcctt gaagcttata aagataaagt ttacacaagg
    19201 gggatctgga taagagatta actattgata ttaaaaaggc aataatttat aaaatttgta
    19261 caaaaaaatg ttaaattttc acattttcat agtatactgt ttaatagaaa gtaggttctt
    19321 tgtaaccgct ccattataaa atcctttcac tcttccggta tactattagg aaaaacataa
    19381 ctggaggata ttttgagctc acacctcatc gttccagtgg ctgttcccca agctcgtgta
    19441 ctgcccaaaa aagttagaca ttctatttag aacgaggatt gagttctgta ttgtacaagg
    19501 ctcggtcctt ttagagtcag cttaaggctg gcttttttat tttttctaac tatcagaata
    19561 ttttgacaaa tgattgtaaa taatgatata atatgcaaaa gctaggaggt ggtcagatga
    19621 ttgagagaat ggaactgggg gaattttaca aggaattacg tttggcgaga aagctcaagc
    19681 agtcagatgt ggcttgtgag ggtctaacag cttctcagtt gtccaagttt gaactagggc
    19741 agtctatgct gtctgcggat aagttgattc tagctatcca aggtataaat gtgacctttg
    19801 atgagtttgg gcacaagctc aacaactatc aagaatctcc acatatgcga atcggtcgaa
    19861 aggttgtgaa tcgctttgcc catcaggata tagctgcttt agagcagcta ttggaggaag
    19921 tcgatcaaga acagatggcg cagacctatc gtcgtttgaa tgccatcgtg attaaagatg
    19981 ccatccattc actaaataaa agctacccac tagcagagga ggatagcgag tttttgacca
    20041 cctatctcta tgctatcgag tcttggacct ggtttgaact ctatctcttt tgcaatacca
    20101 tgcccttctt gagcaatcaa gatctgattt ttttatcaac ctccttactc gaaaaatcca
    20161 aagaatttaa agagttagta cacaatcgat tgtatatgaa gcaaggactc ttaaatatct
    20221 tatcagaact catggagcga aaacttttct cctacatccc aatcttcgaa gccgagttgg
    20281 agaggatgtt acgaccgtat gatgtttttg aaaaagtatc gtggcaattt ttaaagaaga
    20341 tgagtgtctt tcttcaaaca aagggaagca atcaaaaaga gattgaacgc tttatccaat
    20401 ctctgcaggt attagaaaat ccacaattaa tagccctttt tgaattgcgt tttcagcaat
    20461 ataaagaact tatagattag ttagaaatgg caaaataata ataatctcag caacgatttt
    20521 caataatgtt aaaatttgtt tcttcatatt tctccactcc tcattagtag taactttatt
    20581 ataccagaga agtaatcaaa ttcaccaaaa tcgcaaatct gaaatttcta taagaaaaaa
    20641 tatcaaatat gcgattttct aaaataagcc aatgttcgtg ttatactgta cttgtaaagc
    20701 acttgaagca aatcctaggt cgcagaagtg gtttacaaat gaagataatt gaaggagtgt
    20761 aaaagatgtt aaatttacaa tttgcagaaa caatggaatt gacagaagct gagttgcaag
    20821 atgttagagg aggcaacctc gtaaattcta tgggaggtgg tggaagaagt ggtatatctg
    20881 gttggggcgt cccaggaatt taccctggtt ggggcaatca agggatgagt cctaatcgtg
    20941 gagcttttga ttggactata gatttagcag atggattgtt tggccgccgt agaagatagg
    21001 ctcttatagg agataatagg atgaagaaaa tgaaagaagt gaaatttcac ctagcaactg
    21061 gcttgttgat tttgacttat tacctcattt ttaatgtcac atcagactta gattttatgg
    21121 ttgctttgtc tgataatatg tactatgtgt tccaagtttt attggtactt attctgggaa
    21181 ctatagctac aattgctttt gtcaaaagtg aacattggaa agagtgtggt cgtttccagt
    21241 ttcgttggtc ttacctgggt gttttccttc tttctttttt cttattgttc gtgtgggcaa
    21301 atctaacaac ctatatcttt cctcgtaccc agaatggaag tacagtcgta gaagtagcca
    21361 ccaatttaac aggaattagc tattttgtta cgcgaatact ttatactagt attatcgcac
    21421 ctgtatcaga agaggttgtt tgtcgtggtc ttttgatgac aagtttatct aaagttaaga
    21481 gatactatct tgatgttctt gtttccgctg ccatatttgg cgctatgcat gttttgcagt
    21541 atggttggat aacgacagat ttcataaagt attttggaat gggcttgata ttttgtatga
    21601 tgtttcgcta cacccgatcc atctattggg ctatagctct gcatgcttca tggaatagtt
    21661 tcttactcat tgttacttta ttggtatttg gatattagaa aggtgatata tgaaaaagat
    21721 aatctcacac cgctacttta ttatagtttt tctactagtt attgctgacc agaagttcag
    21781 tgttctagtt ttacgcagcg accttgttac tgggctatct gactttgcct attatctgtc
    21841 ggatatgatg ttgaattttc ttgtggtttt atttgctctt attgctatga tttggtcggg
    21901 gaaatggcag aaaatcaata gtagaaagtt taaaggatcc tatcttttct attccttcct
    21961 agctctgctt gcttttgttg tttggaattt cgttaccttt tttcttttcc cacctactcg
    22021 aaatgaaatt tcttatcaac atgctgctcc gacttttaca ggggctacag catttttgat
    22081 gtattttttt tatcctgtga ttgcaggtcc catttttgaa gacatgattt atcgtggatt
    22141 agtgatgact gctctggaaa aaggaaagaa atgggggcta gatgtacttg gttccgctgt
    22201 tttatttggg gtctcgcaca ttagtaatca cggttgggtc ttgacagact ttgtcttcta
    22261 tatgggtgga ggtcttatat ttgcagtctt atttagaatg acaaagtcaa tttattggcc
    22321 tattggactg catatagtct acaatggcat tggtcagctt ttgatgttac tgtaattttg
    22381 gttgttagta gtatcgcggt ctatccttgg ggaaagattc ccatttgaac ttaagaagga
    22441 gttgtgatga aagtatttct tcaaaataga gattttaggc aattaactat caaccagtgg
    22501 atttcaacgc ttggggatac gattttttat ctggccttcc tgaattatgt ggcagatgca
    22561 tcttttgccc ctttggcgat tttactcatc acgatttcag aaaccctgcc ccaagttcta
    22621 caaatctttc tgggagtttt ggcggatttt caacatcatc gtgtcttgaa atacacagtc
    22681 attagttttg caaaattttt gctttactct atagtgtctt tatcactttc agggcagtct
    22741 ttctccttgt tgctagtagc atttatttgt ctgattaacc tcttgtctga cacattgagt
    22801 tatttttcag gcgccatgct cactccgatt ttcattagaa ttattgggca agaccatctg
    22861 gcagaagcta ttggctttaa acagtcaact gttagtttag tgaaaacaat cagtaatatc
    22921 ctaggaggag tcttactagg cattctatcc atccagttta tttccttact gaacgctctt
    22981 acctttttaa tcgcattttt aggtatcctc ttcataaaaa ctgacctctt gaaagtagaa
    23041 aaaacgatta gctatcaaga aggactctct gtaaaatcct tttgccagca tttgctccaa
    23101 tcatcgaaat tgatatggaa tatgaataag gtgctcttgg ttttgtttat tatctctact
    23161 agtcaagcag tgataaatgt tacggttcct atttccactc tatttttaag gaaccagccc
    23221 tttttgaatt tacaaactgg tcaatctctt gccttgttag ccacactaga attatcagcc
    23281 ctgattgtcg gaagccttgt aagtggctat ctgcaacata ccatttccat aaaaacagct
    23341 ctctatgcct cacttgtcat ccagttgctt cttctagtag gatttgccac agttcgtttt
    23401 gactggattc tcatctttag taccttggat gcctttttcg caggtgttct ctctcctaga
    23461 ttgcaagaac tcgtttttaa acaaatacct gaggagtcaa tgggagcagt tcaatcctct
    23521 atcggtgcca ttacggttgt tttacctagc ttatttacaa tagctttggt aaccattgct
    23581 actagctttg gaactctggc agttagcttt gttttattgc tatttcttct agttgccttt
    23641 gttatgcttc tgaatattcg tgaaagtatt tagtggagaa tttctataat atatgtttga
    23701 gactggattg ccagtctttt tatgatggtc aaaatcaaca gacttttttc tcttttctga
    23761 atgtgttata atagactgaa tctaaaatag tacgaaacaa ttgctaaaac atttatagaa
    23821 attaatttta ctttcccaat cgatttgttc tcatcttatt tcaatctgct atataatagt
    23881 ccatgctgtg gttggattct tttcagccta cttgtactct tcgaaactct cttcaaacca
    23941 cgtcagcttc actttgccgt aggtatggtt actgacttcg tcagttctat ccacaacctc
    24001 aaaacggtgt tttgagctga cttcgtcagt tctatccaca acctcaaaac agtgttttga
    24061 gctgacttca tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag
    24121 tttcctagtt tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat
    24181 gaaagaaaaa ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt
    24241 cagtatcggc ctggcctgtg gaattatcgg tgcgccctat gtgacacctg ttgagatggg
    24301 cttgatgagt ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt
    24361 ggttcaagct cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt
    24421 tttgttgagt ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg
    24481 tatgtctagt atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac
    24541 agacaaggta aatcctatgt ggatgcacgg aaacaatctt aacagctatg tggcttggtt
    24601 tgtggggaca gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg
    24661 cttggatttt gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat
    24721 gcaaagacgg attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt
    24781 ctttttgctc ttgacagtga tgtctcagtc actagctgtt ctgtttgcga cgctacttgg
    24841 ttgtagcatg ggggtggttt tagatggtca gtaagtatct tttattagca gttattttct
    24901 ctggcttggt gacttggatt ccccgtatga ttcccttcat cttggtcaag tataagggcc
    24961 tgcctgcgat tgttgagcgt tttttgaagt ttttgcctgt ttccattatc tttgccctga
    25021 ttctttcaag cgtagtgaca ggtaaggttg gcagtcttcc tcaaatcaaa tgactggact
    25081 tcttagccgt ctttccaaca gcttgggtag cctttcgcta ccgcaatcta gtcggaacag
    25141 ttctctttgg agtggtcttg attgccatct tgcgtttggt cttttaaatt acccataaaa
    25201 aaaacttatc acagagatag atatcatata atggcgtaaa tgctcttttt ctgttaagat
    25261 tataaggtat tctattttgg aggaaatgac atgaaaaaaa tcgttaaata ctcatctctt
    25321 gcagcccttg ctcttgttgc tgcaggtgtg cttgcggctt gctcaggggg tgctaagaaa
    25381 gaaggagaag cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc accaaagcca
    25441 tttatctatg aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt tcgcgctatc
    25501 tttaaagatt ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc aggtgtcttt
    25561 gctggtcttg acgctgatcg ttacaatatg gctgtcaaca atcttagcta cactaaagaa
    25621 cgtgcggaga aatacctcta tgccgcacca attgcccaaa atcctaatgt ccttgtcgtg
    25681 aagaaagatg actctagtat caagtctctc gatgatatcg gtggaaaatc gacggaagtc
    25741 gttcaagcca ctacatcagc taagcagtta gaagcataca atgctgaaca cacggacaac
    25801 ccaactatcc ttaactatac taaggcagac ttccaacaaa tcatggtacg tttgagcgat
    25861 ggacaatttg actataagat ttttgataaa atcggtgttg aaacagtgat caagaaccaa
    25921 ggtttggaca acttgaaagt tatcgaactt ccaagcgacc aacaaccgta cgtttaccca
    25981 cttcttgctc agggtcaaga tgagttgaaa tcgtttgtag acaaacgcat caaagaactt
    26041 tataaagatg gaactcttga aaaattgtct aaacaattct tcggagacac ttatctaccg
    26101 gcagaagctg atattaaata atttcttgaa atcatccatt ctgagtaagg tgggtggttt
    26161 ctcagttttt agggatatag ttttaaacta tatatctgac tatctaataa caatttgtga
    26221 aatcaaacaa attgtgagat actagtacgg tattattttt aaggagaaag aatcatgaaa
    26281 atcaaaaaat ggcttggtct agcagccctt gctacagtcg caggtttggc tcttgcagct
    26341 tgcggaaact cagaaaagaa agcagacaat gcaacaacta tcaaaatcgc aactgttaac
    26401 cgtagcggtt ctgaagaaaa acgttgggac aaaatccaag aattggttaa aaaagacgga
    26461 attaccttgg aatttacaga gttcacagac tactcacaac caaacaaagc aactgctgat
    26521 ggcgaagtag atttgaacgc tttccaacac tataacttct tgaacaactg gaacaaagaa
    26581 aacggaaaag accttgtagc gattgcagat acttacatct ctccaatccg cctttactca
    26641 ggtttgaatg gaagtgccaa caagtacact aaagtagaag acatcccagc aaacggagaa
    26701 atcgctgtac cgaatgacgc tacaaacgaa agccgtgcgc tttatttgct tcaatcagct
    26761 ggcttgatta aattggatgt ttctggaact gctcttgcaa cagttgccaa catcaaagaa
    26821 aatccaaaga acttgaaaat cactgaattg gacgctagcc aaacagctcg ttcattgtca
    26881 tcagttgacg ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt ggactacaag
    26941 aaatcacttt tcaaagaaca agctgatgaa aactcaaaac aatggtacaa catcattgtt
    27001 gcaaaaaaag attgggaaac atcacctaag gctgatgcta tcaagaaagt aatcgcagct
    27061 taccacacag atgacgtgaa aaaagttatc gaagaatcat cagatggttt ggatcaacca
    27121 gtttggtaat aagaaacagg gaggtgggag agaaaattcc acctcttgct tttgtataga
    27181 gtatagattg taaagaagac tattcgttca tagaaaggta gagagaatat ggtttttcct
    27241 agcgaacaag aacagattga aaaatttgaa aaggatcatg tagcccagca ttattttgag
    27301 gttttgcgta ccttgatttc taagaaatca gtctttgccc agcaggttgg actcaaggaa
    27361 gtcgcaaatt atctgggtga gattttcaag cgtgttggag ctgaagtgga gattgatgag
    27421 agctatacag cgccctttgt catggcacat ttcaagagtt cgcgtccaga tgccaagacc
    27481 ttgattttct ataaccacta tgacactgtg ccagcggatg gggatcaggt ctggacagag
    27541 gatcctttta cgctttcggt ccgcaatggc ttcatgtatg ggcgtggggt tgatgacgac
    27601 aagggtcata tcacagctcg cttgagtgct ttgagaaaat atatgcagca ccatgatgat
    27661 ttacctgtca atatcagctt tatcatggag ggagcggagg aatcggcttc aacagaccta
    27721 gataagtatt tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt ggtctgggaa
    27781 caagggacca aaaatgcctt ggaacagctg gaaatttctg gtggcaataa ggggattgtg
    27841 acctttgatg ccaaggtaaa aagcgctgat gtggatatcc actcgagtta tggtggtgtt
    27901 gtggaatcag ctccttggta tctcctccaa gccttacagt ctcttcgtgc tgcggatggc
    27961 cgtatcttgg ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg agaaatggcc
    28021 ttgctagaaa cttatggtca acgaaaccca gaggaagtta gtcggattta tggattggag
    28081 ttgcctctct tacaggagga gcggatggcc tttctaaaac gtttcttttt cgatccagcg
    28141 cttaatatcg aaggaatcca gtctggttat caaggtcagg gtgttaagac tattttacct
    28201 gcagaagcca gtgccaagct agaggttcgc ctggttccgg gcctagaacc gcatgatgtt
    28261 ctggaaaaaa ttcggaaaca gctagacaaa aatggctttg ataaggtaga attatactat
    28321 accttgggag agatgagcta tcgaagcgat atgagcgcac cagccattct caatgtgatc
    28381 gagttggcca agaaattcta tccacagggc gtttcagtct tgccgacgac agcggggaca
    28441 ggacctatgc atacggtctt tgatgcccta gaggtaccaa tggttgcatt cggtctagga
    28501 aatgccaata gccgagacca cggtggagat gaaaatgtgc gaatcgctga ttattacacc
    28561 catatcgaat tagtagagga gctgattaga agctatgagt agagatatta tcaagttaga
    28621 tcagatcgat gtgacttttc accaaaagaa gagaaccatc acagcggtta aggatgtgac
    28681 cattcacatc caagaagggg atatctacgg aatcgttgga tattctggag caggaaaatc
    28741 aacccttgta cgggtgatta atctcttgca aaaaccatct gcagggaaaa ttaccattga
    28801 cgacgatgtg atttttgacg gcaaggtgac cttgacggca gagcagttgc gtcgtaaacg
    28861 tcaagatatc ggaatgattt tccagcattt taacctgatg agccaaaaga cagcagagga
    28921 gaatgtagcc tttgccctta aacactctga actcagcaag gaagaaaaga aggctaaagt
    28981 agctaagttg ttggacttgg ttggtttggc agatcgtgct gaaaactacc cttcacaact
    29041 atctggaggg caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg atccaaaaat
    29101 cttgatttca gacgagtcaa cttctgccct tgatccgaag acaaccaagc agattttggc
    29161 cttgttgcaa gatttgaacc aaaaattagg cttgactgtt gtcttgatta cgcatgaaat
    29221 gcagattgtc aaagacattg ccaaccgtgt tgcagttatg caggatgggc atttgattga
    29281 agagggtagt gtgcttgaaa tcttctcaaa ccctaaacaa cctttgactc aagactttat
    29341 ctcaacagct acaggtattg acgaagccat ggtcaaaatc gagaagcaag aaatcgtgga
    29401 acacttgtct gaaaacagtc tcttggtgca actcaagtac gctggagctt caacagacga
    29461 gccacttttg aatgaattgt acaagcatta ccaagtaatg gctaatattc tctatgggaa
    29521 tatcgaaatt ctcgatggta ctcctgttgg agaattggtg gtggttttgt caggtgaaaa
    29581 agcagcgttg gcaggtgccc aagaagccat tcgtcaagca ggtgtacaac taaaagtatt
    29641 gaagggagta cagtaagatg gaatcattga ttcaaaccta tttaccaaat gtctataaga
    29701 tgggttgggc tggtcaggca ggctggggaa cggctatcta cttaactctt tatatgacag
    29761 ttctttcctt cattatcgga ggcttcttgg ggctagtggc aggtctcttt ctcgtcttga
    29821 cagcgccagg tggtgtcttg gagaataaag tcgtattctg gattttagac aaaattacct
    29881 caatttttcg tgcggttccc tttatcatcc tcttggcaat cttgtcacca ctttctcact
    29941 tgattgttaa aacaagtatc gggccaaatg cagcccttgt cccactttct tttgcagtct
    30001 ttgccttctt tgcccgtcag gtgcaggttg tcttggctga attggatggc ggtgtcattg
    30061 aggcggctca agcgagcgga gcgactttct gggacatcgt gggtgtttac ctatcagaag
    30121 gtcttccaga tttgatccgt gtgacgactg tgaccttgat ttcccttgtt ggggaaacag
    30181 ctatggccgg tgcggttgga gctggtggta tcggtaacgt agccatcgct tatggattta
    30241 accgctacaa tcacgatgtg accatcttgg caaccattat tatcattttg attatctttg
    30301 caatccaatt cttgggagat ttcttgacta agaaattgag ccataaataa aaaagagccg
    30361 cgtggctctt tttaactgat cagattttct gggcaaattt tttactcaag gcttgtccaa
    30421 tcaaggcacc cactagggct ccgatgacaa tacttgcgat aaatagaagg acagttccag
    30481 ggtttggagc gaccatgata cggtcgatat attcttggga ttttcctctt gccagaagag
    30541 tagccatata ggctttgggc gcaatccaca taagcaagat tggtcctgtt gtactaaagg
    30601 cgaaaataat gaaagaaagg aagttctttg ttttgtcctt gtattttcct aaatgagcta
    30661 ctccatctgc taggaggcca cagataattc caggaaggaa ggaaccagca ccgtgtttag
    30721 ttcccaagaa aaagagggca atgacaaggc cgatagtggt aatggctcca aagcgcggaa
    30781 cttttgcgac taggatcata tagacgctac cgccgacaag ggcagtaaag gcaggcgcat
    30841 aaaacatgtt tccagtttgg tcaaagagat tgcccaaaag gacaccaatc cccatgcaga
    30901 ggaagtaaag aactgcaaaa agcagtgtag ttaagataga tttcttcatg aattgtctcc
    30961 tgataatttt ttcacaattc tcattttatc atggtttggt gggattgtaa atggatacta
    31021 gaagtttttg aaaacgcttg aaatatgata taatggtttc atagttattt ttaggggaat
    31081 atcatgggta gatttttaga ctttgtcttt aatcgtttct ttttagggat gattgcgaca
    31141 gccttctttt ggctattaac tttagcagga gggattatcc ttggtctagc gccggctagt
    31201 gccaccttga tgagcttata tgcagaacat ggttatagct ttcgggaata cagtttgaag
    31261 gaggcttggt ctctttacaa gcaaaatttt gtctcaagca acctgatttt ctatagcttt
    31321 ttaggtgtgg gtctagtttt gacctatggt ttgtatctct tggtgcaatt gcctcatcag
    31381 accattgttc atttgattgc gacccttttg aatgtcctag tagttgccct gatctttttg
    31441 gcttatacag tatctttaaa attacaagtt tattttgcct tgtcctatcg aaatagtctc
    31501 aaattatcct tgattggcat ctttatgagt ctagcggctg tggctaaggt tctccttggg
    31561 actgtgctac ttgtagcaat tggttattat atgcctgccc tgctattttt tgtaggaatt
    31621 gggatgtggc atttctttat cagtgatatg ttggaacctg tctatgaaat catccatgaa
    31681 aaattggcga caaaatagaa tgaagcagtt ttggctacat acgcttctaa gaacctatag
    31741 ttcagtgatg atcattatca ttgcgagttt tgcaatctta ctctcttacg ctgactggga
    31801 ttcacgtgaa aaggaagccc agagagtagc ccagcgtgta actgctagaa cagtgagtga
    31861 aattgaatat taccatagag agtcaaccca gatagctcag gctttagttg aaaatcaagc
    31921 tcgtatcgag ggaatctata aatactttag ccttagcatg ccagactatt tttactggca
    31981 attagagcgg aaagcttcgc cttatatatc agtctctctg tatgaaaatg ttgatgacct
    32041 ctatgttcga aatgattttg taactggggt ggccattgct tttcaagatt acaaggaagt
    32101 ctatgtttct actaaagaca aacgtagtgg agaaaaaatc agggctgagg atttcaaacc
    32161 agcaggaaat agttttgcca ttccagtgtc agatccagtg tcagatcaag acttaggagt
    32221 gatttacatc tccttggatc ctgctgtttt ataccatgcc attgataata ctagaggtca
    32281 tactccgatg gcagtaacag tgacctcacc ttttgatacg gagatttttc atatgggtga
    32341 gacagttgat aaggagagtg aaaattggct agttggctta acttctcatg gatatcaggt
    32401 tcaggtggca gttcctaaaa actttgtttt acaaggaaca gtgactagct ctgctttgat
    32461 tgtgggtttg agccttctct ttattgtcat tctttatctg actttgaggc agacttttgc
    32521 taattaccaa aagcaggtag tggatttagt agaatccatt caagtcattg ctcaaggcga
    32581 agaggggcgt cggattgaca tttccgagaa agatcaggaa ttactcctaa tcgcggagac
    32641 gaccaatgat atgttggatc gattggaaaa gaatatccat gatatttacc agttagagct
    32701 tagtcaaaaa gatgccaata tgcgagcctt gcaggcgcaa atcaatcctc attttatgta
    32761 taatacgctg gagttcttgc gcatgtatgc agttatgcag agtcaagatg agttggcaga
    32821 tatcatttat gaattcagta gtctcttgcg taacaatatt tccgacgaaa gagagaccct
    32881 cctcaaacag gaattagaat tttgccgtaa atacagctat ctctgcatgg ttcgctatcc
    32941 caagtccatt gcctatggtt tcaagataga tccagagtta gagaatatga agattcccaa
    33001 gtttaccttg caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc acaggcggac
    33061 agataatgtg attagcatca aggctcttaa acaggatggt tttgtggaaa ttttggtggt
    33121 cgataatggt agaggaatgt cggctgaaaa gttggcaaat atccgagaaa aattaagtca
    33181 gagatatttt gaacaccaag ccagctacag tgatcaaagg cagtctatcg ggattgtcaa
    33241 tgtacacgag cgttttgtgc tctattttgg agaccgctat gccattacta tagagtctgc
    33301 agagcaagcc ggtgttcagt atcgtattac aattcaagat gagtagaaag ggagaaaatg
    33361 tataaagtat tattagtaga tgatgagtac atggtgacag aaggtctgaa gcgtttgatt
    33421 ccctttgata agtgggatat ggaggtcgtc gcaacagcca gtcatgccga tgaagctcta
    33481 gaatatgttc aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat gccagacaaa
    33541 acagggcttg atatgattcg ggagatgaaa gagatcttac cagatgctgc ctatatcctg
    33601 ctctcaggtt atcaggagtt tgattatgta aaaagagcaa tgaaccttag tgtggtggac
    33661 tatttggtca agcctgttga taaggtagag ctgggaaatc tgctggagaa gattgcaggt
    33721 cagctcggcg agagagggaa gaaaagtcag actcttagtc aagaattaga cgaggctgga
    33781 tttgttagtt atttagggga taaggagaat tggtggatag gtctatccaa ggaaaaacaa
    33841 ggttccttca ccattcccta ctatgtcttg ggtcaagact ggcagatttt catttctggc
    33901 caccccctag atggtttagt cgttacacct tttgaagctc cttatcaaga acactttgaa
    33961 cgctggaagc tgaatgctga gaaaaccctc ttttacggtt ctgtaaatct gcagcagtct
    34021 gagagtctct ttgcctatta cgaaccgatt tatagggtta tcattcaggg aaatctcaat
    34081 caaatcgtag aagagttaaa tctctcggag aaggtagttc ttgaaaatac acctcgtgtt
    34141 tcgattacta aacagctttt tatccagttt gtcatggatg ttttccattt atttgaacat
    34201 ttcaaagctg atgatatgac ggacattgtc aaaaccattc atgctattca atccttcgat
    34261 gaattggttt cttatatcaa ggaaactctg atcagctttt tcggtcaata ccgtatgaat
    34321 gaaaatgtgg tcagtgtgct ggaagtcatt ggtcgtgatt accaaaaaga gctttccctc
    34381 aaggatatca gtaaggccct ctttatcaat cctgtctatc tagggcagtt gattaagcgt
    34441 gaaaccgatt cgacctttgc agagttacta aacaaacaac gtattaaggc tgcccagcag
    34501 ctcttgcttt caactagtga cagtatcgaa gatatttgtt atgctgttgg ttacagtaac
    34561 cttggatatt tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa agcctaccga
    34621 aaacaggtag aaactatact ataagatttg tattccttta caaaaggtgc tataatatta
    34681 tcaggaggtc ctatcatgaa aaagaaacca atttatctat gggttttgct aatcttgtct
    34741 gcccttattt cagttccgtc tctgtttgga attgtgagtc ccttgcctag taaagaagcc
    34801 cttcgtgctg ctcagaaaca agttgcaggg gtcaatgctc agcaattaga agaccagctt
    34861 aattatacct atagagtagc agaagcatct cattctattt ttaatgttgc tttgattgtg
    34921 ctatctacta ttttagtggt cgtagcgatt gtcttccttg ttcgtaaaaa tttgcaatat
    34981 gcaaactata cttacgttgg ctatgttttg ttagctatta ttggttcgat ttatggctat
    35041 gttggtttac aagacgctgt gcagttggtt caagatgaga gcatgcgttt gacagtgagt
    35101 attggttcaa aagctgttag cattttctat atcgtcatca atgttctctt cctagccctt
    35161 gtcttctata agatgtggcg acaacagaaa gccttggcag aagaagagga aacagaagag
    35221 cttacctaag tattgacaaa aaagaactat caagatacaa tcttggtagt tgttttttga
    35281 ttatagataa aatcatggag gtacatatga agacaatttc tttagtttat attagtttga
    35341 gtggcaacac tgaaagtttt gtgacgcgct tgaaagacta tctcttgtcc cagtacaaag
    35401 ggattgaggt tcaaaagatt catatcaagg atttggtcaa ggaaggcaaa aatttctatg
    35461 aaatggacca tccctatgtc gcttttttgc cgacctatct cgaaggtggg aatggcgtgg
    35521 ataacggaga tgttgagatt ttgacgacac cggtgggaga ttttattgcc tatggtaaca
    35581 atgctagtaa gtgttttggt gtggttgggt caggaaatcg taactttaat aaccaatact
    35641 gcctaacagc caagcaatac agtcaacgct ttggcttccc tgtattggct gactttgaaa
    35701 tgcgaggcat gctggaagat atcaaacatg tcgcagctat tatcgcagat ttgtatgagt
    35761 tggaaaagga gaattaatac tcaatgaaaa tcaaagagca aactaggaag ctagtcgcag
    35821 gttgctcaaa gcacagcttt gaggttgcag atagagctga cgtggtttga agagattctc
    35881 gaatagtata aagttcttat aagaggtggt tgaagttgtt caactgtttt ttgagtataa
    35941 acagtcttta agaacgtaaa aaagcataag ttcaggtctt caaaaccttg ttcttatgct
    36001 ttttatcata gaaatatttg ctgaattttc tcttgaaatg aaaccttatc ttcatctcgc
    36061 gcttgtccct taccctgctt ttccgattgc gagtgcgtag ataaagaaga tggcgaagcc
    36121 aaagaggaaa atcaatcccg caatggcaag gtgtttgagc caagaaggaa gatttttgtt
    36181 ttcacgcgtt accaaggcgt aattcaaaag agcaaagaaa ggtgttgtca ggaaagagcc
    36241 aatcatggca aagcggagca tggttgaaac ctgaccagcg aagaacttga taatgacgat
    36301 accgatgata gcagtgatgg tcatccagat gttcaaagat ttacgattgt cctctttttg
    36361 actgattagc agtcggagag attcctgatt aacgcgagaa tagccatcga taacagttat
    36421 aactgttcca aagatacaga ggaaggcaat aaaggtaatc aagtaacggg accattcgcc
    36481 aagaacagag gcatacatgc ccacgaattg agagatgtat ttggctgaag cagcttcaac
    36541 cgcctgccct gtaggatact gaatcagtgc tcccagtgcc acaaagaaga cggctaggat
    36601 agctgttcca atataaccag tgttaaagtc aaacagagcg tcttctgtgt taaagttgac
    36661 ggtctttctc ttttcagctg accaaagtga attgatggct gaaatttcaa taggagccgg
    36721 catccatcct aggagggaga cgatgaaggg cagagctgcc atttgccaag gtgtcttctc
    36781 gacaaaatca gaactgtatt ctggatgctt gaccgccgca atgataactg caagaacagt
    36841 cgcaatggtt aaggcagaca taatccattt gaccatgccg tctaaaagtt tgtagcctcc
    36901 aaagagtagc atagcccaaa tgattgcaac gagaatgagg gaccactgag taatgctaag
    36961 tccaatcatt gggaaggcac tggcgatgat agctgagcac agaatggcaa caccagccgt
    37021 gttgaccata gccgaaaaga cattgaggat aaagaaaatc cagagataga gttttccttt
    37081 ttcggcataa ccttcaacca aagtctttcc agtatcagct gtgtattcag caccaaaacg
    37141 gaaaaatgga tatttaaaga cattggctaa gatgaccaag agaagtagag accaaccgta
    37201 agaaccgcca gcttgagttg aggatacaat gtgggaacct ccaacagcgg cagttgccat
    37261 taggattcca ggtcccattg cttttagctt gcttgcccag gttgattgat ttaaggaaat
    37321 agcttgcgac atgataaata ctcctttttg aatttttcag aataatctgt atatgtaatc
    37381 tatattctac aaaaaatttt ggaaaaatgc aagaatattt tgaaaaaaga tagaaaattt
    37441 acaaagaaaa tctgaaataa ctgttatgat atagcagatt gaaactagaa tagtacacct
    37501 ttgcttctaa aacattgtta gaaatcgatt tgactgtcct gatcgattta tcctgttctt
    37561 atttcatttc actatagtaa ggtgcagcag gataaagaaa aaccgagaag tttctttctc
    37621 ggatttgctt attatgaggt tcctattttc tatgctccaa gagttgattg atgtgatcta
    37681 cttttttagc ctctctttta tagagaatag cccagatgat gagatagaca atcgcaaact
    37741 cgataatgag ctggaggtag aaaatccagt ggaaagggaa ccaaccagca agagttgcta
    37801 gtgggacaaa gccagccagc ataaggaaga aatgagtcag agtggcacga agcatgctcc
    37861 agtcacggct aaataagcgg ttgccaaagt tgaagagcat accgatggtt gcccagataa
    37921 gtgtgcagta gagcaagacc agggcaccgt gaacctgatg ctgatccatc acttggccta
    37981 tgagagagta gggatttagt ggtgcgtagg tatttggtgc ataaatgagt gaaaagagga
    38041 tagagaggat gaggccgata agaacaccgg tagctgcatc gtgaaatact tgttttttca
    38101 tagttctaat ttctccttga tggtttttag ataacggcgt gaagagtagg tgaagctttc
    38161 gtttttcaag aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag agatggaatc
    38221 gatattgatg atttctgatt gggaaatttg gataaaattg gttggcaaga gtttaagaac
    38281 ctgatagagt cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta gaaccttccg
    38341 attctcgata tagaagcgct gaatcttgcc aatctcaact agatagacct gatcatcgat
    38401 ttttcctttg attttttctc tttggtccag attttctgcg aactcgatga ctttctggac
    38461 tttttcggtt tcttgaggtg cttggacaat cagcttttcc tcctcgtaag tctcactaat
    38521 ctgtagttct actttcatag ttttctctcc ttttcagtta tacaaggttg tgatcacttc
    38581 ctgtatatcc ttattaaaca ctattttata gcccatggca aggaaccttg tctatgtgga
    38641 gggattgggc tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca
    38701 ctaatcaatt tctaggaaaa tagatacgga aaggggctga aagaactagg ggaacagttg
    38761 gaattagaaa gtgataggta tgaaaagtgg cgttgaagct gaataagttt ataaagcgtt
    38821 tgaatgaaga aatggaaagt aaataagttg acgatgaatt acaaaataga ttttatctac
    38881 aatttgtatc tttcaaatca ttgatttgaa ttttagattt aatcatgtta caattaaaac
    38941 tgaaatcaaa tagaagagga aaatgcaaac gtgggcacac tattagcaac aagattaaaa
    39001 aatagacgga aagaattaaa aatgtctcag cgagaactag ctgaaggtat ctgtaaacag
    39061 gggcaaatca gtcgattaga gagcggagag tttacccctg gagcggattt cctgaatgcc
    39121 cttgctaaga agttaaaagt cagtatggat tatttttttg atgagcaggt tgtagagaaa
    39181 gttgaggagt tatcagaatt taagaaatta gcacagacat ttattacaaa tcgcaattat
    39241 gagtctttga aatatatata tgaattggag aatgtaaaag ctcaccgcct atctttagca
    39301 gataaaatct acatggaatg gataaaatct ctgatagatt tttattttta cggacagaaa
    39361 gaagatgcga ttgtacgatt agagggggta ttatcacagt taagtgtatc tgatttgaac
    39421 tatctccaag tttcaaacac cctattcaat ttttattatg atattggaga tttaggtcgt
    39481 ttcaataaaa ttcgtgaaaa actagagtat caagtaaatc acctcaattt gagtacaatc
    39541 gaagagttag aactatctat taagtttaac tacaacgttt gtcgttatct atggttacag
    39601 aaaaatattg aagaagctat tactaaaatt acagctacta taaaacaatg taaggcttac
    39661 agaacgaatt atcttttggc tgatttgtac ttattgatgg gaaatgtaag tgaaaatttt
    39721 tcttctaaaa gttcggtgaa agaatatttt gagacggcat attttctata caaacttgag
    39781 gagaacatgt caatgactct gaaagttgag cattacattg cagatataac agaataatga
    39841 atacggtgtt caagtgagca tcgtttttca aacctagttc agatacaaaa aagtatcaaa
    39901 caaaaaaaaa taaaaaaaca ttataaaaac aattgacaag ctaattttga agtgataaaa
    39961 taaatgcaag aaaacaaaag gagaggtata tgagaagttt gtttaagaaa atagttgctg
    40021 tattagttat tggcttaatt ctgttaggaa ttgcgagaac acctcaagtt cataagatgg
    40081 ctagagggat tgatcctggt cctgcgaatt ttatttagaa atcaatttct actaattgag
    40141 tattaatggg taaaaagatt gtatggataa gaaaaattta cttgtattgg ttactggttc
    40201 ggtaggtgcc agtaatgtag atacatactt atattacata aaaaaattct ataatgttaa
    40261 agtgatactt tctgaaaatt ctaaaaaatt tataagtaag gaattaatta gttatttttg
    40321 tgataaagtt tatgatgaaa tatttgttga agaggtagtt ccgcacgttt ttctgccata
    40381 tgaatctgac ttacttctta ttttaccagc tacggcaaat gtgattggca aaattgctaa
    40441 tggtattgct gatgatttag ttacagcaac tgttttaaac tttaataaaa aaataatttt
    40501 ttgtcccaat atgaactcta ctatgtggga caatcacata gttcaaagaa atgtatcaat
    40561 tctaaaggag ttgggacata tatttttatt tgagtctaaa aaaacatatg aggtaggatt
    40621 gcgtaaagca atagattcaa catgttcaat gttacaacca cagtcgttag taaaagaact
    40681 tatcaaatta gaaaatattg tccttgaaga gggacattaa aaactactga gaatattaat
    40741 gaggggaaaa aatggaaaat tcatcaatcg atgtagatat gctgttggaa gaattgacac
    40801 aagaagcaat ggtcgttgtt gctgttgata aggactgtta atttaaactt atggcaatat
    40861 atgaaaggtt actggatgtt ttaaattatg caggcagtag ccttttatta tatacaaatg
    40921 gataaagtaa ggataataca atgattaata aaaaaataca acaagttgtt ttggaatcat
    40981 tacagaattt tttgaatggg aacttcattt cgccttgtgt agtctatgat tttggcttgc
    41041 tggaaactgt acttgatgaa tttaaaaatc aaattcctgt aacattcaat taccaacttt
    41101 tttatgccgt taaagcaaat tcaaatgaga agatacttga attcttagta gataaaattg
    41161 atggagttga tgtggcgtca ttatctgaat tagatgtggc taaaaaattt ttcccaccaa
    41221 ctcaaatttc tgttaatggt cccgcatttt cttatgaaac tttatataat ctgattaaaa
    41281 aacaatataa agttgatatt aactttttgg aacatcttca acaattttcc ccaaaagaat
    41341 ctgttggaat aagagtaacg gagccagatg aacttaataa tcgtatgagt cgatttggaa
    41401 taaatatttg cagtgataat tggactagta atttacaaaa tcctttaatt acacgactgc
    41461 attttcattt tggagaaaaa gatgataaat ttattgttaa gttagataaa atattattta
    41521 agttacaaga aattaataaa cttagagagg ttagagaaat aaatcttgga ggcggtttta
    41581 tgaaattatt tatggaaaat cgtttgaaag aattttttct atcacttatg gaaatctata
    41641 aaaagtacga tattgatagt actgtgacta caataataga accaggtagt gcaattactt
    41701 cattttctgc ctatatgatt actagcccag ttaatgttag tgaggtgaat gagcagcagg
    41761 ttatcacgtt agacacatca atatacacca atacattatg gtttgttccg catattatta
    41821 caacgttaaa ttcaagtagt aaagagcgtt atagtactat tctctatggt aatacctgtt
    41881 atgaacatga caagtataaa atgaaagttt cgcttccaag gttaactcaa aatagcagta
    41941 tagtgttttt tcctgtagga gcttatataa aaagcaatca ttcaaattta catcgtaatg
    42001 attttatgcg ggaggtatat ttgtggacaa aaaacttgac atattagata aagttaagga
    42061 atatttagga aataaaacta ctcaaattct ggataatcaa tataaagaat ttttgaaact
    42121 taatgatata aggcgagcgt ttggtatttc agaaaaagta ttaaacaatt cttttaattt
    42181 tacgagtaaa gaatttaatg atttaattaa taacgaaaat tatttattcg aatatgcatg
    42241 tagaattaga gaggaatgga gaaaaaaatg ctttaatcat tcttatcgtt ttctatgctc
    42301 acctataatt acagatgatt ttcttaacac gaagacattg agaagtagcc aaattgaata
    42361 taaatatgag cgatatttat cgaaaagttc gataggcgat agagcggttg atggctttgt
    42421 ttccttcaat actttaacag ctaatggtat gtctgctatt aaactatgtc ttgagatatt
    42481 aaactctatt ttcttcaaga agaagattga tttattatat tcaaccggat attatgaaac
    42541 aagattttta ttaaataatc ttgctaaatc aggtattagt tgctatgagg taagtaattg
    42601 tgaattggat aaagataaat tttataatgt attcatgatg gaacccaatc gagccgattt
    42661 aacattacaa aaaactgatt tcaagatagt agaatatttt gttaagtata aaaataattc
    42721 aataaaagtc gttattttag atatttcata tcaaggttct aattttaaat tagtagaatt
    42781 tttagagaaa tttaaatttg cgaatgtaat tatttttgtg gtacgatctt tgataaaatt
    42841 agatcaaatg ggattagaat tgacaaatgg gggaataata gaagtgttta ttcctaatca
    42901 tttgagaaag ttgaaaaatt ttattgaaga ggaattcaat aaatttagaa attctcacgg
    42961 agctaatcta agcctctatg aatactgttt gcttgataat tctttaactt taaaaaatga
    43021 ttggaactat tctgatttag ttatgaaatt tacgagtaat ttttatgctg atataaaaga
    43081 cttgttcatg gaaaattctg atattgaaat catccatgaa gagggagtac cttttgtatt
    43141 tttagattta ataggtgaag gtaaaaaaga atatgaaatg ttttttcaat ggttaaactt
    43201 cttttacaaa cagcttggaa tcacattgta tgctagaaat agttttgggt ttcggaatct
    43261 aacagtagag tattttggaa ttattgggac agaaagatat atatttaaga tttgtccagg
    43321 tgtttataaa gggttaagtt attatttgat gaaattttta ttaaaatctt tttcaaatga
    43381 atatttaaaa actactgatg aggttaatag atgaaaaatt tgataaagtt gctaataatt
    43441 agattgattg ttaacttagc agacagtgta ttttatatag tagcattgtg gcacgttagc
    43501 aataattatt cttcgagcat gttcttagga atatttattg cagtaaatta tctaccggat
    43561 ttgttactaa tcttttttgg accagttatt gacagagtaa atccgcaaaa aattcttata
    43621 atatcaattt tggttcaatt agcagtggct gtaatatttt tattattatt aaaccaaata
    43681 tcattttggg tgataatgag tctagtgttt atttcagtaa tggctagctc cataagttac
    43741 gtgatagaag atgtgttgat tcctcaagtg gtagaatatg ataagattgt atttgcaaat
    43801 tctcttttta gtatttcgta taaagtatta gattctattt ttaattcatt cgcatcattt
    43861 ttacaggtgg cagtaggatt tattttattg gttaagatag atataggcat atttttactt
    43921 gctctattta tattgttgtt gttaaaattt agaactagca atgcgaatat agaaaacttc
    43981 tctttcaaat attacaagag agaagtgttg caaggtacaa agtttatttt aaataataaa
    44041 ttattattta aaaccagtat ttctttaacg cttataaact ttttttattc atttcagaca
    44101 gtagttgtac cgattttttc tattcgatat tttgatggtc cgatttttta tggtattttt
    44161 ttaactattg ctggtttggg tggtatattg ggaaatatgc tagcgccaat cgtaataaaa
    44221 tatttaaaat cgaatcaaat tgttggtgta tttctttttt tgaacggctc aagttggtta
    44281 gtagcaattg ttataaaaga ctatacttta tcacttattt tatttttcgt ttgttttatg
    44341 tctaaaggag tcttcaatat tatttttaat tcgttgtacc aacaaatacc tccacatcaa
    44401 cttcttggta gggtaaatac taccattgat tctattattt cttttggaat gccaattggt
    44461 agtttagttg caggaacgct tattgatttg aatattgaat tagtgttaat tgctattagc
    44521 ataccttatt ttttgttttc ttatattttt tatacggata atggattgaa agaatttagt
    44581 atatattaga aatgtttatg ttcattcaaa agcataatga ctataactga aaaagaaaag
    44641 tgatatcttt aaggttgttc ttcttggtgg tgagattcgt gagacaaccc aagcttttgt
    44701 cggaaagatt accaatgctt tgatggatag gatgtacttt agcaagatgt ttttagtggt
    44761 aacggtatcg tggatggacg tgtaataacc tcttctttcg aggagtattt tactaaaaaa
    44821 ctagccttgg agcgttcccc agaaacggac ttactcattg actcttcaaa gatttgggga
    44881 gaagattttg cttcatctgt tccttgaaaa aagtcacagc agtcatcaca gacgatagta
    44941 ctgaacaaaa ctatgaagag ttagaaattt atacgcaggt gattgtataa aggatctgga
    45001 aatagataag aagttgatta gtattgacct aggtggtaca aatattaaga ttactgttct
    45061 ttcaaatgac ggtgagattg aaactttgtg gagtattaca acagatacaa gtgagaaagg
    45121 ttctcaaatt atatcggaca tcatcagttc tattaaaaat aaattgaccg aacggaatat
    45181 tcctgatagc gaccttcttg gaatcggtat gggaagttgc tcatcatact ttccttgtaa
    45241 atcatagggg ctataaactc tccgtctact tgtcctgcaa caattgaagt ctgctcaaaa
    45301 cgccgtccgc taatcttttc atagactttc tcccttttag gagcctagct ttctagtttg
    45361 ttctttgatt tttattgagt ataccactat tttactccct ctggcaaggg actttgtcta
    45421 tgtggaggga ttgggctcct atgtggtgga gcttttctgt tctttctgaa atatggtata
    45481 atagcactaa tcaatttcta ggaaaataga tacagaaagg ggctgaaaga tgtctcatat
    45541 tattgaattg ccagagatgc tggcaaacca aatcgcggct ggagaggtca ttgaacgtcc
    45601 tgccagtgtg gtcaaagagt tggtagaaaa tgccattgac gcgggctcta gtcagattat
    45661 cattgagatt gaggaagctg gtctcaagaa ggttcaaatc acggataacg gtcatggaat
    45721 tgcccacgat gaggtggagt tggccctgcg tcgccatgcg accagtaaga taaaaaatca
    45781 agcagatctc tttcggattc ggacgcttgg ttttcgtggt gaagccttgc cttctattgc
    45841 gtctgttagt gtcttgactc tgttaacggc ggtggatggt gctagtcatg gaaccaagtt
    45901 agtcgcgcgt gggggtgaag ttgaggaagt catcccagcg actagtcctg tgggaaccaa
    45961 ggtttgtgtg gaggatctct ttttcaacac gcctgcccgt ctcaagtata tgaagagcca
    46021 gcaagcggag ttgtctcata tcattgatat tgtcaaccgt ctgggcttgg cccatcctga
    46081 gatttctttt agcttgatta gtgatggcaa ggaaatgacg cggacagcag ggactggtca
    46141 attgcgccaa gcaatcgcag ggatttacgg tttggtcagt gccaagaaga tgattgaaat
    46201 tgagaactct gacctagatt tcgaaatttc aggttttgtg tccttgcctg agttgactcg
    46261 ggctaaccgc aattatatca gcctcttcat caatggccgt tatattaaga acttcctgct
    46321 caatcgtgct attttggatg gttttggaag caagcttatg gttggacgtt ttccactggc
    46381 tgtcattcac atccatatcg acccttatct agcggatgtc aatgtgcatc caactaagca
    46441 agaggtgcgg atttccaagg aaaaagaact gatgactctg gtttcagaag ctattgcaaa
    46501 tagtctcaag gaacaaacct tgattccaga tgccttggaa aatcttgcca aatcgaccgt
    46561 gcgcaatcgt gagaaggtgg agcaaactat tctcccactc aaagaaaata cgctctacta
    46621 tgagaaaact gagccgtcaa gacctagtca aactgaagta gctgattatc aggtagaatt
    46681 gactgatgaa gggcaggatt tgaccctgtt tgccaaggaa accttggacc gattgaccaa
    46741 gccagcaaaa ctgcattttg cagagagaaa gcctgctaac tacgaccagc tagaccatcc
    46801 agagttagat cttgctagca tcgataaggc ttatgacaaa ctggagcgag aagaagcatc
    46861 cagcttccca gagttggagt ttttcggaca aatgcacggg acttatctct ttgcccaagg
    46921 gcgagatgga ctttacatca tagatcagca cgctgctcag gaacgggtca agtacgagga
    46981 gtaccgtgaa agcattggca atgttgacca aagccagcag caactcctag tgccctatat
    47041 ctttgaattt cctgcggatg atgccctgcg tctcaaggaa agaatgcctc tcttagagga
    47101 agtgggcgtc tttctagcag agtacggaga aaatcaattt attctacgtg aacatcctat
    47161 ttggatggca gaagaagaga ttgaatcagg catctatgag atgtgcgaca tgctcctttt
    47221 gaccaaggaa gtttctatca agaaataccg agcagagctg gctatcatga tgtcttgcaa
    47281 gcgatctatc aaggccaatc atcgtattga tgatcattca gctagacaac tcctctatca
    47341 gctttctcaa tgtgacaatc cctataactg tcctcacgga cgtcctgttt tggtgcattt
    47401 taccaagtcg gatatggaaa agatgttccg acgtattcag gaaaatcaca ccagtctccg
    47461 tgagttgggg aaatattaaa agtataaaaa agtctgggaa aaattttcaa aatcaaaaaa
    47521 acgcataaaa tcaggtgttc aaaaaccttg attttatgcg ttttatcatg gaaatagtta
    47581 cttcattttt tcctaattct tttcgaaact ctttttaaac gacgtcagtt ttatcagtaa
    47641 tctcaaaaca gtgttttgag ctaattttgc cagttttgtc tgtaacatcg aagttgtgtt
    47701 ttaccactct gcgactggtt tcctagtttg ctctatgatt ttcacagagc attaaattgc
    47761 gattttgcca agtttcttta ttcgtctaaa agtagagtct gttctatgcg tctaatgtac
    47821 gaatcaggtt gaccatttca atagctcctt gtgcacactc agaaccctta tttcctgctt
    47881 tagtaccagc tcgttctatg gcttgttcaa ttgtatctgt cgttagcaca ccaaacataa
    47941 caggaatttc gctatttaaa ctgatttggg cgattccctt agatacctcg ctacatacat
    48001 aatcataatg acttgtattc cctctaatga cagctcccaa gcagataatt gcatcatatt
    48061 ttttactttt tgccattttt gatgcaatca gtggtatttc aaaagctcct ggaacccagg
    48121 ctacctctat atctttctcg tttacattct ctcttttgag attatctagt gctccagata
    48181 ataattttga agttataaat tcattaaatc tcgctacaac aatacctatt ttaatattgt
    48241 ttgctactaa attaccttca taagtgttca tttatttttc ctccatattt aaaatgtgac
    48301 ccattcgatt tttctttgtt tctaaataaa aactatcgta aggattggct tctatttcga
    48361 ttgatattct actggaaatg gtaattccat atttttctaa ctgttcaacc ttgtcaggat
    48421 tatttgtcag taaatgaagt gactgaagtc ccagatcttt aagcattttt gctccaatat
    48481 gatattctct taaatcacct tcaaagccta atgcaagatt ggcatcaagc gtatccatgc
    48541 cttgatcttg taaatgatag gcttttaatt tattgataag tccaattcct cgtccctcct
    48601 gtcgcaagta aagtaagaca cccgaaccat tctcaacaat cattttcata gccttatcga
    48661 attgctgtcc acaatcgcaa cgtaaagagc ctaaaacatc tcctgttaaa cattcggagt
    48721 ggacccgaca taatacattg gcttcatcct ctatatttcc cataataaga gcaagatgat
    48781 gttccccatt tagtttatct atatagctaa ttgctttgaa attaccgtat ctagtaggca
    48841 tattgacagt tgaaactcgt tctaccagct gatcatatac ttttctatat tcttgtaatt
    48901 ctttgatggt aattagtgga atgttgtgtt ttttcgagaa ctgaattaaa tcatctgttc
    48961 tcatcatttt gccatcatga ttcattattt cacaacatag gccacactct tttagtccag
    49021 ctaattttaa taaatcaaca gttgcttctg tgtgtccatt tctttctagg acaccacctt
    49081 tttttgcaat taaaggaaac atgtgtcctg gcctgcgaaa atcagagggt gttatatctt
    49141 cagctacaca catacgtgcg gtcagtcctc tttcctcggc agaaatacct gtggtcgttt
    49201 ctttataatc aattgaaact gtaaaagcag tcttatgatt atctgtattg ttttcaacca
    49261 taggtgaaag cattaattga ttagctaaac tttcgctcat aggcatacaa attaatcctt
    49321 tggcataagt agccataaaa ttaacatttt ctgttgtagc tgcttgtgca gaacaaatta
    49381 agtctccttc attttctcta tccttgtcgt ctataacaag aacaagtcgt cccttctgca
    49441 atgcttctaa tgcttcttgt atttttcgat attccattga ctgattatcc tttctgctaa
    49501 aatccatttt gatataatag ttccttagat atttctgatt ttggagagtt atccatcagt
    49561 ttttgcacat atttacctaa gatatcattt tcaagattta ctgtactccc gacttgttta
    49621 ctcttaagaa tggtttgttc caaggtatga gggataacag atactgaaaa gtttactttg
    49681 gagactttag cgacagtcag actaatgccg tcaattgtaa tagatccttt ttcaactatt
    49741 aaatctaaaa tttctttttg tgtgttgatt tgataccata cagcattatc atcttttttt
    49801 attgacgaga tttttcctgt accatcaatg tgtcctgtaa cgacgtgacc cccaagtcga
    49861 ccgttgacag ataaggctct ttctagattc acctcacttc catgttttaa tagagtaaga
    49921 gctgttcgac tccatgtttc attcattaca tcaactgtaa aggattgatg attgaaatga
    49981 gtaactgtaa gacagatacc atttactgct atactatcgc ctaaatggat atccgttaat
    50041 atttttgagg ctttaattga tagtttacaa ttacgagagt ctttctgtat tctttcaact
    50101 tttccgattt cttcaattat tcctgtgaac atggataaat cacttcactt tctatgagat
    50161 agtcatttcc tatttgagaa aatgcataag gtttcaatct aatagcgtca tttggcaaag
    50221 aaataccttc acctccgaca ggaaacttgg cactacctcc aaaaactttt ggtgcaatat
    50281 atattttcag ctcatcaaca atttgttgtt ccaaagcact ccaattcatt agactgcccc
    50341 cttctagaac taggctatca atctgcatgt ttcctagatg ttgcattaaa ctcgataagt
    50401 ctatatgatt gccttttttc tttatggaaa gtatttcaca gccatgattt tgatatagct
    50461 tcattttatt tttgtcttca gaggaagtgg caatgtaagt tttaatatca tttgctgttt
    50521 ttacgatttt agaggtaaga ggagttcgta aatgtgtatc gcatatgata cggataggat
    50581 ttttcccttc ctccaatcta catgtcagca aaggatcgtc ttgaataaca gtattgactc
    50641 ccaccataat tgcactaaca tggtgtcgta actgatgcac atgctttctt gcttcttctt
    50701 cagtaatcca tttggattga tttgttttag tggctatttt tccatccatt gacattgcat
    50761 atttcataaa aacatagggt acatgctggg taatatactt tctaaaactt tttattaagt
    50821 taagacactc attttctaaa attccaacag taacttgaag attattttcc tcaagtatct
    50881 ttactccttt tccagataca ataggattac agtctaggct tccaatgact actcttgtaa
    50941 taccactatc gattatagca tctatacagg gaggtgtttt cccgaagtga caacagggtt
    51001 caagtgttac ataaagcgtc gctccgacag gggattctct acagttttta agagcatttc
    51061 tctcagcatg tgggccacca aaaaactcat gataaccttg tccgataatg tgattatctt
    51121 ttacaataac tgcgccgacc ataggattgg gattgacgta accagcccct ttttgtgcca
    51181 gttttattgc taatttcata tattttgaat cgctcatctc gctacctcca aaaaaatata
    51241 ccttgaatag gggactactc aaggcataca aaagaaaact tatgcgatta acaaaaatgc
    51301 tctgaaatga caagtaatca tttcagagca cgcaaaaagc acaaatatac ttttatcttc
    51361 tttcatccag actatactgt cggctttgga atttcaccaa atcatgcctt tcggctcgtg
    51421 ggctatacca ccggtaggga atttcaccct gccctgaaga tagttattca attacagatg
    51481 attatagtac ttaattttga atatgtcaac agataaatac cgattgtttt tgatatactg
    51541 tatttgtgat aatcgattct cgctcctcgg ataaagaaaa tatgatatac tagataaacg
    51601 aaataagaga gaaggaatac tatgtacgca tatttaaaag gaatcattac caaaattact
    51661 gccaaataca ttgttcttga aaccaatggt attggttata tcctgcatgt ggccaatcct
    51721 tatgcctatt caggtcaggt taatcaggag gctcagattt atgtgcatca ggttgtgcgt
    51781 gaggacgccc atttgcttta tggatttcgc tcagaggatg agaaaaagct ctttcttagt
    51841 ctgatttcgg tctctgggat tggtcctgta tcagctcttg ctattatcgc tgctgatgac
    51901 aatgctggct tggttcaagc cattgaaacc aagaacatca cctacttgac caagttccct
    51961 aaaattggca agaaaacagc ccagcagatg gtgctggact tggaaggcaa ggtagtagtt
    52021 gcaggagatg accttcctgc caaggtcgca gtgcaagcaa gtgctgaaaa ccaagaattg
    52081 gaagaagcta tggaagccat gttggctctg ggctacaagg caacagagct caagaaaatc
    52141 aagaaattct ttgaaggaac gacagataca gctgagaact atatcaagtc ggcccttaaa
    52201 atgttggtca aataggagca gagaatgaca aaacgttgtt cgtgggtcaa gatgaccaac
    52261 ccgctctaca tcgcctatca tgatgaggag tggggccagc ccctccatga tgaccaagta
    52321 ttgtttgagt tgttgtgtat ggaaacctat caggcaggcc tgtcttggga aacggtactc
    52381 aacaaacgcc aagctttccg agaagtcttt catagctatc aaattcactc agtcgcagag
    52441 atgactgaca ctgaattgga agccatgctg gagaatccag ctatcattcg aaatagagcc
    52501 aagctttttg ctacacgcgc taacgcccaa gcctttctac agttacaggc agagtacggc
    52561 tcttttgatg cctatctttg gtcttttgtt gaggggaaaa ctgtcgttaa cgatgttcct
    52621 gattatcgcc aagcgccagc taaaacaccc ttatctgaga aattagccaa agatctcaaa
    52681 aaacgaggct tcaagttcac aggcccagtc gccgtattgt cttttctaca ggctgcaggg
    52741 ctagttgatg accacgagaa tgattgtgag tggaaaggtc ttaaatgatg tctaacaaaa
    52801 ataaggaaat tctgattttt gcgattctct atacagtcct ctttatgttt gatggcgtta
    52861 aattgctggc ttctttaatg ccatctgcca ttgcaaatta tcttgtttat gtagttttag
    52921 ctctatatgg ctccttcttg ttcaaggata gattgatcca acaatggaag gagattagaa
    52981 agactaaaag aaaattcttc tttggagtct taacaggatg gctctttctc attctgatga
    53041 ctgttgtctt tgaatttgta tcagagatgt tgaagcagtt tgtgggacta gatggacaag
    53101 gtctaaatca gtctaatatt caaagtacct ttcaagaaca accactactg atagctgttt
    53161 ttgcttgtgt cattggacct ctggtagaag aattattttt ccgtcaggtc ttattgcatt
    53221 acttgcagga acggttgtca ggtttactaa gcattattct ggtaggactt gtttttgctc
    53281 tgactcatat gcacagtttg gctctatcag agtggattgg tgcagttggt tacttaggtg
    53341 gaggccttgc cttttctatt atttatgtga aagaaaaaga gaatatctac tatcccctac
    53401 ttgttcacat gttaagcaac agcctctcct taatcatttt agctatcagt atagtaaaat
    53461 gaaatgagaa caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
    53521 tagtttcaat atactgtaat atgtgatgaa aatgccagta atgataccga gaaaaaagct
    53581 gagaaacttt tcccagcttt atttgttata gtcaaagaga atgacttgtt cctgtgcatc
    53641 tacatgagca tggaccccaa agggtacaat tgctcttgga gttgcgtggc cgacattcag
    53701 attatagaca atcgggatat tgctgtcaat gatatccaat agtgcctctt tatagtcgtc
    53761 atggaaagtt tcatccatag gttttccgac caagagtcca ttgatgaccg cgaatatgcc
    53821 agtgtccttt aaagttagca acatcttttt gaagtcttct ggcttaggct tttcttcgct
    53881 tgtttcgagc aagaggattt tcccttccca gtctgacaag tcagggaaaa gtttgtattt
    53941 ttggcagagt tccgtgctat ctgcgtatcg agagttgtca aagatatcgt agagggattc
    54001 gaggcaacca ccgaggattt tcccctcgaa ctgggcactt ccttgcaaca agtcaaaacc
    54061 tgtatttgta tgactgacac gaggtgttcc cagggccgtg ggactaaaat cagttcgttc
    54121 ctcataccaa acgtcactag ggcggatttc tgaaattctt cccgtctcaa tcaattcttt
    54181 aaagtagtga aggctatagg ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa
    54241 ggattgacca taaaaagtct tgattcctaa tttatgcaac atgaggtggt tcatggttgt
    54301 atccgagaag ccaagaaaaa ttttttgctt gataaccttt tggagttggt cattttcaaa
    54361 aagataaggt agcaagcgat aggtatcgtc tccaccgatg gcacatagga tcatgtcgat
    54421 gctatcatca gaaaaggcat gaatcaaatc ctctgcacga gcttcaggat ggtccttgat
    54481 aaagtctaat ccttttaacg aatggggcaa aaagatggga ttgagtccca gatccttgag
    54541 acgttggata cccaagtcca cttcgtgttt gacaaaatcc tctccgataa tgccactaga
    54601 taaactaaca ataccaatag tagaaaccat atctcatcct cctagaaata gattgagcct
    54661 attttatcac aaaatataag agaaagctat gtgggatttc agaagaatac ttttaatact
    54721 cttcgaaaat ctcttcaaac cacgtcagtt ttatctgcaa cctcaaagct gtgctttgag
    54781 caacctgcag ctagcttcct agtttgctct ttgattttca ttgaatataa ctcaaaaaag
    54841 taagtgaaaa agcaaccgca cctgcagttg cttttatcgt tttcttattc ttgatagcgt
    54901 gttggtcctg cacttgcact acggatattc aagcgtttct tgagatagaa gcgaagggct
    54961 aggagggctg ctccaataat agctagtggc aatggatcca gtactgggtt aaggctagct
    55021 ggtaggaagc ttgttgcaaa gaagacaagc aaccaaagga acatagaagc taggataact
    55081 agtacagatt tccagaaagg tggacgttga ctgcgatcca tatctggtcc atagtattgg
    55141 taaacaaagt agtacatcaa gtagaaggca aatccaccaa ccagtccaac taatagaaga
    55201 gtaatcaatc catagccgaa agcttggtct gccgcaaaga aggttgtgag ggcgctgaca
    55261 agggcaaaga ggctagtgat gaaaagagct gagtccataa tcatcagttt tgggtcatca
    55321 ttttcttttg gatgctcttt ttcgtactgc tctttgacag tgaagctatg agcccaatga
    55381 gttggtgcgc cgtataggga acgggcagtt gtacctttag attgctcctc aaggatttgg
    55441 ggaatgactt cctcaaaaat agtctggatt tcagcgtctg ttttcccatc tttgatgaat
    55501 tgttgggtag caatgtggac aaactcttgg tttttcttag ttaatttttg tagatcaatt
    55561 tgagacatag gaactcctct tagaaccatt ttttatggat gagatagaga gtgagcgaga
    55621 cactcatagc aaaggcgata aagacgatta accagaaggc atttggctct ccgtttaggg
    55681 ggatttcatt atccttaaag ttcatcccgt aggcagaaaa gaccatggtt gggatggaca
    55741 tgacgatggt cacaagggcc aaggttttca tgatgttgtt ctggttgtta gaaatgatag
    55801 aggcaaaggt ctctgtcata gaatgcaaga cgtttccata aatatctgcc atctcgatgg
    55861 cctgttgggt ttcaatcagg gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga
    55921 tattgctggt tgaactggtc aatttcttaa tcacgcgctc atttgttttg agggaggcct
    55981 tgaaatagac gatagttttt tccaattcca tgagctcaat caattcttca ttacgagttg
    56041 attgatgcag ttgactttcg atttgttcac tcttgcggtc gattgaacga agggctgtta
    56101 ggtaaagctc tgcattgcga taaagaattt gaaagataaa acgtgaacgc atgaaggtat
    56161 agaaattacg caatcgacgg ttgataaaga catcaaggac aggtagtggt tccaaacacg
    56221 tagtgataat ggtttcctca gtgatgataa taccaagcgg gatggttacg tagtaggtgc
    56281 ggttatttct ttcctccgtg accggcacgt ctacgataat cagggtatac tcgtcttcaa
    56341 tggtaatacg agacatttct tccgcatcga gcggtgctcg aaggtcagca atatcaatat
    56401 cgaaggtgtt ggcgatttcg agtgattcat tttgagtcgg attgacgaga ttgatccaag
    56461 tacccggttc aagcgtatcg atctctttaa attcagttgt tgtagagaga aaaacttgtt
    56521 tcatatccct tatcctttct catttttcag attttttcac actgtactat tatactacaa
    56581 aatcggcctt ttgggtaata gaatctcact ttttttggta taatggtaag caataatgga
    56641 ctagaaagaa caaagatgca agataaaatt gtcattcatg gggcgcgtgc ccataattta
    56701 aaaaatattg atgtggagat tccgcgagac aagttggttg tcgtgactgg cttgtcaggt
    56761 tcagggaagt ccagtctggc ttttgatact ctctatgcgg agggacaacg tcgctatgta
    56821 gagagtttgt ccgcctacgc tcgtcagttc ttgggaaata tggaaaaacc agatgtagat
    56881 gccattgatg gtctcagccc agctatttcc atcgaccaga aaacgactag taaaaatcct
    56941 cgctcgacgg tgggaacgac gactgaaatc aatgactatc tgcgtctcct ctacgcacgt
    57001 gtggggacgc cttactgtat caacggacat ggagctatca atgcttcttc tgtggagcaa
    57061 atcgttgata aggttttgga gttacctgaa cgccagcgct tgcagatctt ggctcctgtc
    57121 atccgcaaga aaaaaggcca acataagagt gttatcgaga aggttcagaa agacgggtat
    57181 gttcgtgtcc gtgtggatgg ggaagtctat gatgtgaccg aagtgccaga gttgtctaag
    57241 agcaagcaac acaatatcga tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt
    57301 agtcgtctct ttgattccat tgaggctgcc cttcgtattg cagaaggtta tgtcattatc
    57361 gacaccatgg acgactcgga gttgttgttc tctgagcatt atgcctgtcc agtttgtggt
    57421 tttactgtcc cagagttaga gcctcgtctc ttttctttca atgctccttt tggctcttgt
    57481 agtgagtgtg acggcttggg catcaagctg gaggtggata ctgatttggt agtgccagat
    57541 gctagcaaaa ccttacgtga gggagcgcta gcaccttgga atcctatctc atccaactac
    57601 tatccaaaca tgttagagca ggccatgaaa gtctttggag tggctatgga taagcctttt
    57661 gaggacctgt cagaagaaga taagaacttg attctctatg ggtcagatgg taaggaattc
    57721 catttccact atgagaatga atttggtggc gtgcgcgata tcgacattcc gtttgaggga
    57781 gttatcaata atatcaaacg tcgttaccat gaaaccaata gcgattacac tcgcactcag
    57841 atgcgtctct acatgaatga gctgacctgc ggaacctgtc aaggctatcg tctcaatgac
    57901 caggccttgt ctgtccgtgt gggcggccag caagggccac atattggaga aatctcagac
    57961 ctgtctatcg ctgaccactt ggacttggtg agccagttaa ctttgtctga aaatgaagcc
    58021 atcattgctc gtcccattct caaggaaatc aaggatcgtt tgaccttcct taataatgtg
    58081 ggtcttaact atctaaccct gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag
    58141 cgcattcgtt tggcaaccca gattggttcc aacctatcag gtgtcctcta tatcctagac
    58201 gagccgtcaa tcggtcttca ccagagggac aatgatcgcc tgattgccag tctgaaaaag
    58261 atgcgtgact tgggcaatac tcttatcgtg gtggaacacg acgaagatac catgcgtgag
    58321 gctgattatc tgattgacgt tggtcctggt gccggtgttt ttggtggtga gattgttgca
    58381 gcaggtacac ctaagcaagt ggctcgcaac agcaagtcta tcacaggtca gtacttgtca
    58441 ggcaaacgtg tcatcccagt accagaagag cgccgtgtcg gaaatggtcg ttttattgaa
    58501 gtgataggag cgcgtgagaa caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa
    58561 tttatcgcag tgacaggtgt gtcgggttca gggaaatcga ctctaatcaa cagcattctc
    58621 aaaaaagcca ttgcccagaa gctcaaccgt aattcagaca aacctggtaa gtttaagact
    58681 attacaggga ttgagcatgt cgaccgtttg attgatattg accagagccc tatcggacgg
    58741 acgccgaggt ctaacccagc tacctataca ggagtttttg acgatatacg tgaccttttt
    58801 gctcagacaa atgaagccaa gattcgaggc tacaagaagg ggcgcttcag tttcaacgtc
    58861 aagggaggtc gctgtgaagc ttgctcaggt gacgggatta tcaagattga gatgcacttc
    58921 ttgccagatg tttatgtggc ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc
    58981 ctagaagttc actacaagga aaagagtatt tcgcaggtct tggatatgac ggtcaacgat
    59041 gcggtagaat ttttccaaca cattccgaaa attcaacgca aactccagac catcaaggat
    59101 gtagggctag gctatgtgac cttgggacag ccagctacca ccctttctgg gggagaagcc
    59161 caacgtatga agttagctag cgaactccac aaacgatcga caggaaaatc tttctacatt
    59221 ctggatgagc cgacgacagg gcttcataca gaggacattg ctcgcctgct caaggtttta
    59281 gctcgctttg tagacgatgg caatacagtc ctcgtcatcg agcacaatct ggatgttatc
    59341 aagacggcag accatatcat tgacttggga cctgagggcg gtgtcggtgg tggaaccatc
    59401 atcgtaacag gaactccaga agaagtagct gccaacgaag ccagctatac aggacactat
    59461 ttgaaaggaa agttacatca tgaataaacg cgtacaagca tttctagcta aaatgcaaga
    59521 aaaagaacta gatggtatca tcatcaacaa tcttaaaaac gtctattatt tgactggttt
    59581 ttggggctca aacggaacag tctttatcag tcgtgaccgt caggtcttag tgacagactc
    59641 tcgctatatc atcgcagcta agcaagaaac cagtggtttt gagattgtgg ctgatcgtga
    59701 tgaattggct gtcattgcag gaattgttaa ggacatgggc ttgactcgta tcggttttga
    59761 agatgagatt tcagtgtctt attaccaccg tatgcaggca gcttttgcag gtttgaactt
    59821 gcttccacaa actcagtttg tggaaggtct tcgtatgatt aaggatgagg cagagattgc
    59881 agcgattcgc aaggcttgtt ctatctcaga ccaagctttc cgcgatgcgc ttgactttat
    59941 caaaccagga aaaactgaaa ttgagattgc caacttcctt gatttccgca tgcgtgagtt
    60001 gggagcatct ggcttatctt ttgatacgat cctagctagc ggtatcaatt cttctaaacc
    60061 ccatgcccat ccaatgcaca aaccagtgga gttgggagaa gccatcacca tggacttcgg
    60121 ctgtctctat gaccactatg tcagtgatat gacccggact atctatctag ggcatgttag
    60181 cgatgagcag gcagagattt acaatacggt tctaaaagct aaccaagcct tgattgacca
    60241 agctaaggca ggcttaggtt tccgtgactt tgacaaaatc cctcgtgata ttatcattga
    60301 ggcaggttat ggtgactact ttactcacgg cattggccac ggtattggtc tggatatcca
    60361 tgaggaaccc tactttagtc agacttctac agaaactatt aagacaggta tggccttgac
    60421 cgatgaacca ggtatctata tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct
    60481 gattacagag acaggttgtg aattattgac cctagctcca aaagagttga tagtcattta
    60541 gttgtttgtc aagaaaaaag tacgaaaatg acgaaaaaag tcaaaaaaaa ttaaaaatag
    60601 gtcgcaagtc ggatgttttt tatggtataa tagactaaac tgatagtaac atgtagcgaa
    60661 aggggtaggt acatgattaa aatttataca gtctcaagtt gtactagctg taaaaaagca
    60721 aaaacctggc tcaatgccca ccagttaagt tataaagaac aaaaccttgg taaagaagga
    60781 attacgagag aagaattact ggatattcta accaaaacag ataacggaat agccagcatc
    60841 gtttcgtcta aaaatcgcta tgccaaagcc cttggagtgg atattgaaga tttgagtgtc
    60901 aatgaagttc tcaatctgat tatggaaaca ccgagaattt taaagagccc aatccttgta
    60961 gatgaaaaac gcctgcaagt tggctacaag gaagacgata ttcgtgcctt cctaccacgc
    61021 tctgtccgta atgtagaaaa tgcagaagca cgtttgcgtg cagctctata aacatcaagg
    61081 ctgggagcac ttcccagtct tattctattt taatttcaaa aagaaagaag aaagaaatga
    61141 aaaaaatagt tcttgttagt ctagctttcc tttttgtcct ggttggttgc ggacagaaaa
    61201 aagaaactgg accagctaca aaaacagaaa aagatacgct tcagtcggca ttgccagtta
    61261 ttgaaaatgc tgagaagaat acagttgtaa ctaagacttt ggtcttgccc aagtcagatg
    61321 atggtagcca gcagacacaa acaattactt acaaagacaa gacttttttg agtctagcta
    61381 tccaacaaaa acgtccagtc tctgatgagt tgaagactta tattgaccaa catggagtgg
    61441 aggaaactca aaaagctctt cttgaagcgg aggagaagga taagtctatc attgaagctc
    61501 gtaaattggc aggtttcaaa cttgaaacaa aactattgag cgcaacggaa cttcaaacaa
    61561 cgactagttt tgattttcaa gttctggatg tcaagaaggc ttcccagttg gaacatctga
    61621 agaatattgg tttggaaaat cttttgaaaa atgaaccaag caaatatatt tcagatagat
    61681 tggcaaatgg cgcgacagaa caatagaaaa tccaaataaa tagactgcct gaattgtaga
    61741 aggtaaggaa ttatgctata atggtactat tctaaggaaa gagggtgtta gaatgggatt
    61801 tactgaagaa acagtacgtt ttaaattgga cgattccaat aaaaaagaaa ttagcgaaac
    61861 tttgacagat gtttatgctt cgttgaacga taagggttac aacccaatta accaaatcgt
    61921 aggttacgta ttgagtggag accctgccta cgttcctcgt tataataatg cacgaaatca
    61981 aatccgtaag tatgagcgtg atgaaatcgt tgaggaattg gttcgctact acctcaaagg
    62041 acaaggagtc gatctataac ctatgagaat tatgggattg gacgtcggtt caaaaacggt
    62101 aggggtggcg attagcgatc cgcttggttt tacagctcaa gggcttgaaa tcatccagat
    62161 aaatgaagaa caaggccaat ttggttttga ccgcgttaag gaattggttg atacttacaa
    62221 ggtggaacga tttgtagtgg gcttgcctaa aaacatgaac aatacaagtg gaccgcgcgt
    62281 agaagctagt caagcctacg gagcaaagct agaagagttt tttggtttac cagtagacta
    62341 tcaggatgaa cgcttgacaa cagtggctgc tgagcgcatg ttgattgaac aagcagatat
    62401 cagtcgcaat aagcgcaaga aagtcattga taagttagca gctcagctga ttttacaaaa
    62461 ttatttagat agaaaatttt aatataaagg agaggctatg tcacacgatc ataaccacga
    62521 ccacgaagaa cgtgaactaa tcacactagt agatgagcaa ggaaatgaaa ccttgtttga
    62581 aatccttttg acaattgatg gaaaagaaga atttggtaaa aactatgttc ttctagtacc
    62641 agttaacgca gaagaagacg aagacggaca agttgaaatc caagcttact cattcatcga
    62701 aaacgaagat ggaacagaag gcgaattgca accaatccca gaagactcag aagacgaatg
    62761 gaacatgatt gaagaagtct tcaacagctt tatggaggag tgatacagtc tgggagactg
    62821 tatcagccca actcccagaa atggggaaga gttggaacgt ccagtgaaca tttttacccc
    62881 aactcccaga aatggggaag agttggaacg tccagtggat gtttttagcc catattcaca
    62941 ttcatagtag ctagttatta gctaaccagg taagaggtcg ggacgaaaat cctggccttg
    63001 tttttattag taaccatact ttaatgcgat aattgattgg acttttgtca aggagatgtg
    63061 tatgtttgaa gtagaagaat ggctccatag tcggattggt ttgaattttc gatcaggttt
    63121 gggtcgaatg cagcaagcgg tggatttgtt aggaaatcct gagcagtctt accctattat
    63181 ccacgtaaca gggactaatg ggaaaggatc taccattgct tttatgaggg aattatttat
    63241 ggggcatggc aaaaaagttg cgacctttac ctcccctcat atcgtctcta tcaatgaccg
    63301 aatctgcatt aatgggcagc ctatagcaga cgcagacttt atccgtttga ctgatcaggt
    63361 caaggagatg gagaaaacgc ttctgcaaac tcctgcccag ttgtcctttt ttgaattgct
    63421 gaccttggtt gcttttcttt attttaggga gcaggaggtg gatttggttt tattagaagt
    63481 gggaattggt ggcttacttg acacgaccaa tgtggtaact ggagagtttg ctgtcatcac
    63541 ctccattggg cttgaccatc aagaaacctt gggtgatagt ctagaagcaa ttgcagagca
    63601 gaaagctggt attttcaagg ctggtaagaa ggcagtgatt gcgaaattgc ctccagaagc
    63661 taggcttgcc tgtcagaaaa aagccgaatc tttagctgtt aacctttatc aggcaggtca
    63721 agatttttta atgctgaatg gtgatttttc aagctcttta ctaaatcttt cacagctgaa
    63781 cataggctta gaaggagtct atcagcagga gaatgcagcc ttggcgttgc aaacttttct
    63841 tctttttatg agagaaagaa aggaagctgt tgatgaacag gctgtaagaa aggccttgga
    63901 acagacccat tgggctggtc gcttggagcg tattcgccca cagatttatt tggatggtgc
    63961 tcataacctc cctgccttga ctcgcttggc tgagtttatc aaagaaaaag agcaggaagg
    64021 ctatcgacct caaatcctct ttggatcctt gaaacgtaag gattatcaag ggatgttggg
    64081 ttatctgact gaaaaattgc ctcaggtgga actcaaggtg accggctttg actatcaggg
    64141 ggctttggac gaaagggatg tgacaggtta cgatatagtt tcttcttacc gagaatttat
    64201 cagcgatttt gaagaaaggg cagacgctca agacttgctg ttcgttacag ggtctctcta
    64261 ttttatctca gaagtacggg gctacctgct ggaccgtgag cagataaatt gactccattt
    64321 tttgaacttg ttatactagg ggaattgctg gttagagaaa atttctctaa attggtagca
    64381 gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt ctcttgcttc tctagcaagt
    64441 tttagtctct tagtagcttg ttcacaaaga gctcaacagg ttcaacagcc tgttgctcag
    64501 cagcaggtcc aacaacctgc tcaacagaat accaatactg caaatgcagg aggtaaccaa
    64561 aatcaagcgg ctccagtaca aaaccaacct gttgctcaac cgaccgatat tgatgggact
    64621 tatactggtc aggatgacgg agaccgtatc actttagtgg taactggaac gactggtaca
    64681 tggactgagc tcgaatctga cggggatcag aaagtcaaac aggttacatt ggattcagca
    64741 aatcaacgca tgattattgg cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc
    64801 gtcgtagatg atatggatag agacccatcg gaccaaatcg ttttaactaa ataagactat
    64861 gcaagtagaa agggctggat ttttcagcct ttttactttt acctagaaaa taatcataaa
    64921 tacttgcctt ctaccgaata cctgcgttat actagtatca attacttgtt tttagcattc
    64981 aaactatcaa ggaggggata tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg
    65041 ctttagtctt tcgattatgc tacttgaact ttgtcttgtt tttggtctct ttctttattt
    65101 aggacgcatg gctcccattt tatggattac tgtcctcatt ctactgagta tcatcacaat
    65161 catttcgata gtcaaccgta atacgactcc tgagaataag gtaacctggt tgttagtagc
    65221 ctttgtgcca gtatttggtc ccttgctcta tctgatgttt ggtgaaaggc gattgtccaa
    65281 aaaagaaatc aaacaactga agaagctagg ctctatgcat ttccaagaag caaatagcca
    65341 gctactaaaa gagaaattaa aagaaagtga caaggcagct tatggagtca tcaagtcctt
    65401 attgagtatg gataccaatg ctgacatcta tgatcaaact gcctctacat tttttcctaa
    65461 cggagaagct atgtggaaaa agatggtaga agatcttaaa aaggctgaga aatttatttt
    65521 cttggaatat tacattatag aagaaggttt gatgtggaat cgcatactag atatactaga
    65581 gcaaaaggta gctcagggtg tagaggttaa gatgctctat gatgatatcg gctgtatggc
    65641 tactttaaca ggagattatg cacatcgact tcgtcagctg ggcatcgagg cccataaatt
    65701 caataaagtt attcctcgtt tgacagtggc ttataataac agagatcata gaaaaatatt
    65761 gattgttgat ggtcagatag cctatactgg tggggtcaat ctggcagatg agtacattaa
    65821 ccacgtcgag agatttggtt attggaagga tagtggaatt cgcttagacg gactagcagt
    65881 aaaagctctg acacgcttat ttttgaccac ttggtacatt aatcgaggag aaattagtga
    65941 ttttgatcaa tatcatttag aaaatcattc tatcccgagt gacggtttaa ccattccata
    66001 cggaagtgga cccaagccaa tttttcgagc gcaggtaggg aaaaaagttt atcagagttt
    66061 aatcaatcaa gcaacagaat cggtctatat tacgacacct tatttgatta tagattatga
    66121 tttaacagag acaatcaaaa atgcagctat gagaggggtc gatgttcgaa ttatcacccc
    66181 ttacatacca gataagaagt tcattcagtt agtcacgaga ggagcttatc ccgaccttct
    66241 ttctgctggt gttcggattt atgagtatag tccaggtttt attcatagta agcagatgtt
    66301 ggtagacgaa gattttgcgg tggtggggac aatcaatctc gactaccgga gcttggtaca
    66361 ccattatgaa aatgcagtct tactctataa aactccttct ataagggaaa tcgcccgaga
    66421 ttttcgaaat atatttgcag attctcagga agtctatcct cattctatca aaacgagctg
    66481 gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc cctatcttat aagaaatcta
    66541 ggacttagga tacaaaccag acctattttt cttgtctttt acgaatagaa agaaagagga
    66601 ggggaattca tgctaactaa ggaagaagtg aatgatatga ttgagttcaa acagacacat
    66661 ttacaccgct ttcgggaaat tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag
    66721 ccatcgcaag ctgacaatcc taggactttt tgatatacta agacagataa attgaacagg
    66781 agaaacgata tgagtgtgta tggtagagta gaagaagttc ataaggagaa tcgtgaacct
    66841 ctagaatacc aaatcgaaca agaatcgcat catcgtgaat caagtcgtct tcctttggtg
    66901 aaaattttac tatggagtac gcttgtaacg gggataactc taggagtacc gctattactc
    66961 gacttaatga gtgcacaaga agtgcaggat ttttatgcag gttgggcgct tcatcagaca
    67021 gggaagattt acagcgacta ttatggaagt caaggtttgc tttattattt gctgacttac
    67081 gtgagtcagg gcggattttt ctttgccatc tttgagtggt tagccttggt agcaggagga
    67141 tttttccttt ttagatcagc ggacaccttg acagagcaag gagaccaagc tggacatctg
    67201 gtgactattt tttacatgct agttacaggt cttgcttttg gtggaggcta tgcgactctt
    67261 ttagcgcttc ctttcttatt cgcagccttt agtttagttg cggcttacct aagcaatcca
    67321 agccatgata agggatttgt acggattggg ctagctttgg caggcggatt tttctttgct
    67381 cccttatcat cgctcctgtt tattgctgta gtgagtttag gcttgttggt ctttaacctt
    67441 gggcatagac gctttgcgca tgggttttat cagtttcttg cagtggcttt aggtttttca
    67501 cttgtctttt atccaactgc ctactatagt gctgcaacag gaagttttgg ggatgcgatt
    67561 agtggtattc gttatcctat tgacagtatt cgctttgatt ttacttctaa aattttagag
    67621 aatatgtttt tttatggctt gttgtccctt ggtttgggat ttgtggtttg tatcttttta
    67681 ggtctctttc aatccaaacc ctttaaacta tacgtcattt cggttcctgc aagtcttgtg
    67741 gtaattttag gtttgatttt acttttcttt tcacaagagc ctctgcacgc ttcttatttg
    67801 atggtcgtct tccctgtttt cctactttta ttggtaacca atattaagag tcaacagagg
    67861 gggcgtagtg ctagaagaag ccgaagagaa acgccagtta gcctatggag tcgtttcttc
    67921 aaaggaaatc tatatctgct agtttttggg tttgtctatc ttttgtctgt tccttttttg
    67981 atgaagtttg tcctttatcc agtaccttat caagaacgta atcgtcttgc tgatttggta
    68041 aaagaggaga caaatacgga agatgctatc tatgcatggg atgatactgc gactctttat
    68101 cgtaagagtg agcgcttgtc gccatcggcg attttgtccc cgttgcacta tacagcaact
    68161 gaggaaaatc gtaataagtt acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg
    68221 gtaaatgata aggtggtagt ctggtctgaa gtggaaacac tcttaaaaga aaattaccaa
    68281 caagtaaaga ctgattactc agagtttaaa gtctataaaa ttaaataacc aaatcaatat
    68341 cttgtgtatt tttaaaaatt ttaggatttt taacacaaga tattgatttt tctttttaga
    68401 gtggtataat actttttaga aagaacattt tagaaaagag catgcatatg attgcactag
    68461 aagaaaaaat tacaattttg ccaactctct tcgtcgagaa acgagatggg agacgtgttg
    68521 tatttgatgt ggacaagatt gacaaggctc tccacaaggc ggctgacaag gttatggatg
    68581 tgacacccct ggttgaaaaa tgcctcaatg atctgactga gcgaattatt acagaaattc
    68641 atagtcgctt tccacaggga attaagattt acgaaattca aaatatcgta gaacatgaac
    68701 tccttgaagc caaagaatat gcgctggctg aggagtatat tacttatcgg acacagaggg
    68761 attttgagcg ctcaaaagcg acggatatca actttagtat tcataaactt ctcaacaaag
    68821 accagacagt tgtcaatgaa aacgctaata aagacagtga tgtctttaac actcagcgtg
    68881 atttgacagc agggattgtt gggaaatcaa tcggactgca aatgcttcct aagcacgtag
    68941 ccaatgccca ccaaaagggg gatatccact atcacgattt ggactacagt ccctataccc
    69001 ctatgaccaa ctgctgtttg attgatttta agggtatgtt ggaaaatggt tttaagattg
    69061 gaaatgcaga ggtagagagt cccaagtcta tccagactgc gacagcacag atttctcaaa
    69121 tcattgccaa cgttgcttct agccagtacg gtggctgttc agctgaccgt atcgatgaaa
    69181 ttttggcgcc ttatgcagag aagaattatc aaaaacatct caaagatgca gaagagtggg
    69241 tattgcctga aaaacaggaa gattacgctt ggaagaaagc gcaaaaggac atctacgatg
    69301 ccatgcaatc tcttgagtat gaaatcaata ctctcttcac ttcaaatgga caaacacctt
    69361 ttacttcgtt aggttttggt ctgggaacca gtcgttttga acgagaaatt caaaaagcta
    69421 ttttaaacat tcgcatcaag ggtcttggtt cagaacaccg tacggctatc tttcctaaac
    69481 ttatctttac gcttaaaaga ggcctcaact tagaggaagg aactcccaac tatgacatca
    69541 agcagttggc tctagagtgt gcaaccaagc ggatgtatcc agacgtcttg tcttatgata
    69601 agattgttga tttgacaggt tctttcaagg tgcctatggg ctgccgttct ttccttcaag
    69661 ggtggaagga tgaaaatggt gtagaagtca attcaggtcg catgaatctg ggtgttgtga
    69721 cggttaatct gcctcgtatt gctcttgagt ctgaaggtga tatgaataag ttctgggaaa
    69781 tcttcaacga gcgaatgaat atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta
    69841 aagaggcgac accagcgaat gctcctattc tttatcagta cggtgctttt ggccatcgtc
    69901 taggtaaaga agaaagtgtt gaccagctct ttaagaatcg tcgtgcgacc gtttcgctgg
    69961 gctatatcgg cttgtatgaa gtagcgacag ttttctttgg taacagctgg gaaagtaatc
    70021 cagatgctaa ggaattcacg ctagacatca ttcacgatat gaaacgccgt gtagaagagt
    70081 ggtcagacca atatggctac catttctcta tctactcaac accatccgaa agtctgacag
    70141 accgtttctg ccgactagat atagacaagt ttggctctat tcctgatatc acagacaagg
    70201 aatactacac caactctttc cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat
    70261 tggactttga gaaagtctat ccggaagcag gtgcgtcagg tggtttcatc cattattgtg
    70321 agtatccagt ccttcagcaa aatccaaagg ccttggaagc tgtctgggat tatgcttatg
    70381 accgtgtagg ctatctaggc accaatactc cgattgaccg ttgctacaag tgtgactttg
    70441 aaggggattt tgaaccaact gagagagggt ttgcttgtcc aaactgtggc aatagcgacc
    70501 ctaaaacagt agatgtggtg aaacgaactt gtggctacct aggtaatcct caagcaagac
    70561 cgatggtcaa cgggcgtcac aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa
    70621 cgattaaaat agctgggcat caagtaacaa attagaaaga aatgaaatgg gaaaatatca
    70681 actagacgat aaggggcgcg cacaagtgac ccgttatcac gagaaacact ctaaaggtgg
    70741 agctggtaag aaagaacgct tgcttagctt cagagaacaa tttttaaaca agaacaagaa
    70801 aaaataaaag tgagagccag ctctcgcttt tctcatagtg ggaggtaagg atggaattac
    70861 gcagaccaag attagcggat aagaaagctg ttttagatat gatgacagag tttgaaaaat
    70921 ttcagtcgcc tcacgacggc ggtttctggg atacagagaa ctttgtgtat gaagactggt
    70981 tagaaagcaa tcaggaacag gaaatgggga ttaatctgcc tgaaggatgg gtttctgcaa
    71041 ttcagttagt ggctttttct gagaaaggtc aagcagttgg atttcttaat ctccggttgc
    71101 gcctcagtaa ctttctacta gaagaaggtg gccacattgg ctactccatt cgtccatctg
    71161 aaagaggcaa gggttatgca aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa
    71221 agaacatcaa gaaagctctg gtgacctgta gtgtgaataa tcctgctagc agagcagtca
    71281 ttctagcaaa tggtggaata tttgaggatg ctcgcaatgg agtcgagcgt tattggatag
    71341 aggtagcgaa tgaataatcc aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt
    71401 cgtatcattg actacaaggc ctttaacttt gtggacggcg aaggcgtgcg caactctctc
    71461 tatgtatcag gctgtatgtt tcactgcgag ggatgttata atgttgcgac ttggtctttt
    71521 aatgctggca ttccctatac agcagaatta gaagagcaga ttatggcaga ccttgcccaa
    71581 ccctatgttc aaggcttgac tttgctggga ggggagcctt ttctcaatac tgggattctc
    71641 ttgccacttg ttaagcggat tcggaaggaa ttgccagaca aggacatctg gtcctggacc
    71701 ggctacactt gggaagaaat gatgttggaa actccagata aactggaatt cttgtcactg
    71761 attgacattc ttgtcgatgg aagatatgat cgaactaaga gaaatcttat gctccagttt
    71821 cgaggttcat ctaaccaacg aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta
    71881 gtgatttggg acaagctcaa tgacggaaaa gaaagctatg aacaggtgaa gagagaatga
    71941 agaaaaagga cttagtagac caactagtct cagagatcga gacggggaaa gtcaggacac
    72001 tgggaatata cggtcatgga gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag
    72061 ctttagattc tactacagta aatttgctag agacagatcc ttatatcacc tcaggacgcc
    72121 atctggtagt acccaaggac gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc
    72181 atgaactgga gagtttgcag agagatatcc ttgccttgca ggcgggtatg gatgtcttga
    72241 caattgaaga accttggaag gctagtgagg tcttgtctgg agccaaacca attttgattg
    72301 tcgaagggat gtctgttggc tttctaccca aggaactctt tgaaaaaacc atctgtttct
    72361 acacggatga ggagaccgaa ttaaagcgac gccttgctag agatacgact gtgagaaatc
    72421 gcgatgcatc ctttatattg gctagccatc agatgagacg ggagcagtat ctgcgatact
    72481 ataaagaaac tgagtctaag gcggatatct tagtggacca atcagaagat aaatttgatg
    72541 tcaagaggac ttaacttatg tatagaagaa atatagaaga aaatccctaa ttttagaaag
    72601 aaaaagtagg aaaacagttt catcccaaaa aaacgaaaaa acagcaacaa atcccttgca
    72661 atcgcagggg ctttgtgtta ttctattatg gtgctgtaaa ttacagcctt agttttgatg
    72721 caagaggttg cgacacgctc ggttgcattg ccacgcaaca ccgcgtcggt tttcttgtgg
    72781 agctagccta ttatcttaaa tagacgaaaa ggagaaaaag atggcaaaca aaaaaatccg
    72841 tatccgtttg aaagcttacg aacaccgtac gcttgacaca gcggctgcaa aaatcgtaga
    72901 atcagctact cgtacaggtg cacaagttgc gggtccaatc ccacttccaa ctgaacgtag
    72961 cctctacaca atcattcgtg cgactcacaa atacaaagac tctcgcgaac aatttgaaat
    73021 gcgtacacac aaacgtttga tcgatatcgt taacccaact caaaaaacag ttgatgcctt
    73081 gatgaaattg gatcttccaa gtggtgtaaa cgtagaaatc aaactttaat ctaaaatata
    73141 aaagagcaga ggctggtgtt tcaatctaat tgaacacggg ctaaactcgg tgtgaaaaag
    73201 ataaacttcc tagtgtctgc tagacactgc gtcagtttcc tattttcacg ttgagtttga
    73261 cgccctttgt atcttagact tgagcataaa aaacgctcgt taaaaacttt ttgaataaaa
    73321 aatatagaaa aggaactatt ttctcatgac aaaaggaatc ttagggaaaa aagtgggaat
    73381 gactcaaatc ttcactgaag ctggcgaatt gatccctgta acagttattg aagcaactcc
    73441 aaacgttgtt cttcaagtta aaactgttga aacagacgga tacaacgcta tccaagttgg
    73501 tttcgatgac aaacgcgaag tattgagcaa caaacctgct aaaggacatg tagcgaaagc
    73561 taacacggct cctaagcgct tcattcgtga attcaaaaac gttgaaggct tggaagttgg
    73621 tgctgaaatt acagttgaaa cattcgcagc tggagacgtt gttgacgtaa cgggtacttc
    73681 taaaggtaaa ggtttccaag gtgttatcaa acgccacgga caatcacgtg gaccaatggc
    73741 tcacggttct cgttaccacc gtcgtccagg ttctatgggg cctgttgcac ctaaccgcgt
    73801 attcaaaggt aaaaaccttg caggacgtat gggtggcgac cgcgtaacaa ttcaaaacct
    73861 tgaagttgta caagttgttc cagaaaagaa cgttatcctt atcaaaggta acgtaccagg
    73921 tgctaagaaa tctcttatca ctatcaaatc agcagttaaa gctggtaaat aataaagaaa
    73981 ggggaaatca gtcacaatgg caaacgtaac attatttgac caaactggta aagaagctgg
    74041 ccaagttgtt cttagcgatg cagtatttgg tatcgaacca aatgaatcag ttgtgtttga
    74101 tgtaatcatc agccaacgcg caagccttcg tcaaggaaca cacgctgtta aaaaccgctc
    74161 tgcagtatca ggtggtggac gcaaaccatg gcgtcaaaaa ggaactggac gtgctcgtca
    74221 aggttctatc cgctcaccac aatggcgtgg tggtggtgtt gtcttcggac caactccacg
    74281 ttcatacggc tacaaacttc cacaaaaagt tcgtcgccta gctcttaaat cagtttactc
    74341 tgaaaaagtt gctgaaaaca aattcgtagc tgtagacgct ctttcattta cagctccaaa
    74401 aactgctgaa tttgcaaaag ttcttgcagc attgagcatc gattctaaag ttcttgttat
    74461 ccttgaagaa ggaaatgaat tcgcagctct ttcagctcgt aaccttccaa acgtgaaagt
    74521 tgcaactgct acaactgcaa gtgttcttga catcgcaaat agcgacaaac ttcttgtcac
    74581 acaagcagct atctctaaaa tcgaggaggt tcttgcataa tgaatttgta tgatgttatc
    74641 aaaaaacctg tcatcactga aagctcaatg gctcaacttg aagcaggaaa atatgtattt
    74701 gaagttgaca ctcgtgcaca caaacttttg atcaagcaag ctgttgaagc tgctttcgaa
    74761 ggtgttaaag ttgccaatgt taacacaatc aacgtaaaac caaaagctaa acgtgttgga
    74821 cgttacactg gttttactaa caaaactaaa aaagctatca tcacacttac agctgattct
    74881 aaagcaatcg agttgtttgc tgctgaagct gaataatcta aggaggaaat atcgtgggaa
    74941 ttcgtgttta taaaccaaca acaaacggtc gccgtaatat gacttctttg gatttcgctg
    75001 aaatcacaac aagcactcct gaaaaatcat tgcttgttgc attgaagagc aaggctggtc
    75061 gtaacaacaa cggtcgtatc acagttcgtc accaaggtgg tggacacaaa cgtttctacc
    75121 gtttggttga cttcaaacgt aataaagaca acgttgaagc agttgttaaa acaatcgagt
    75181 acgatccaaa ccgttctgca aacatcgctc ttgtacacta cactgacggt gtgaaagcat
    75241 acatcatcgc tccaaaaggt cttgaagtag gtcaacgtat cgtttcaggt ccagaagcag
    75301 atatcaaagt cggaaacgct cttccacttg ctaacatccc agttggtact ttgattcaca
    75361 acatcgagtt gaaaccaggt cgtggtggtg aattggtacg tgctgctggt gcatctgctc
    75421 aagtattggg ttctgaaggt aaatatgttc ttgttcgtct tcaatcaggt gaagttcgta
    75481 tgattcttgg aacttgccgt gctacagttg gtgttgtcgg aaacgaacaa catggacttg
    75541 taaaccttgg taaagcagga cgtagccgtt ggaaaggtat ccgcccaaca gttcgtggtt
    75601 ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtaaagca ccagttggtc
    75661 gtaaagcacc atctactcca tggggcaaac ctgctcttgg tcttaaaact cgtaacaaga
    75721 aagcgaaatc tgacaaactt atcgttcgtc gtcgcaacga gaaataatat taaactagtc
    75781 gcttaagcaa ctagtaaatc cgccagctcg gtagcgctcc ataggagtgc aagccgctgt
    75841 ggtacaacat ttaaaggaga aaatataaaa atgggacgca gtcttaaaaa aggacctttc
    75901 gtcgatgagc atttgatgaa aaaagttgaa gctcaagcta acgacgaaaa gaaaaaagtt
    75961 attaaaactt ggtcacgtcg ttcaacgatc ttcccaagtt tcattggtta cactattgca
    76021 gtttatgacg gacgtaaaca cgtacctgtt tacatccaag aagacatggt aggccacaaa
    76081 cttggtgaat ttgcaccaac tcgtacttac aaaggtcacg ctgcagacga caagaaaaca
    76141 cgtagaaaat aaggagaaca taaatggcag aaattacttc agctaaagca atggctcgta
    76201 cagtacgtgt ttcacctcgt aaatcacgtc ttgttcttga taacatccgt ggtaaaagcg
    76261 tagccgatgc aatcgcaatc ttgacattca ctccaaacaa agctgctgaa atcatcttga
    76321 aagttttgaa ctcagctgta gctaacgctg aaaacaactt tggtttggat aaagctaact
    76381 tggtagtatc tgaagcattc gcaaacgaag gaccaactat gaaacgtttc cgtccacgtg
    76441 cgaaaggttc agcttcacca atcaacaaac gtacagctca catcactgta gctgttgcag
    76501 aaaaataagg aggtaaaatc gtgggtcaaa aagtacatcc aattggtatg cgtgtcggca
    76561 tcatccgtga ttgggatgcc aaatggtatg ctgaaaaaga atacgcggat taccttcatg
    76621 aagatcttgc aatccgtaaa ttcgttcaaa aagaacttgc tgacgcagca gtttcaacta
    76681 ttgaaatcga acgcgcagta aacaaagtta acgtttcact tcacactgct aaaccaggta
    76741 tggttatcgg taaaggtggt gctaacgttg atgcactccg tgcaaaactt aacaaattga
    76801 ctggaaaaca agtacacatc aacatcatcg aaatcaaaca acctgatttg gatgctcacc
    76861 ttgtaggtga aggaattgct cgtcaattgg agcaacgtgt tgctttccgt cgtgcacaaa
    76921 aacaagcaat ccaacgtgca atgcgtgctg gagctaaagg aatcaaaact caagtatcag
    76981 gtcgtttgaa cggtgcagat atcgcccgtg ctgaaggata ctctgaagga actgttccgc
    77041 ttcacacact tcgtgcagat atcgattacg cttgggaaga agcagatact acatacggta
    77101 aacttggtgt taaagtatgg atctaccgtg gtgaagttct tccagctcgt aaaaacacta
    77161 aaggaggtaa ataaccaatg ttagtaccta aacgtgttaa acaccgtcgt gagttccgtg
    77221 gaaaaatgcg cggtgaagca aaaggtggaa aagaagtagc attcggtgaa tacggtcttc
    77281 aagctacaac tagccactgg atcactaacc gccaaatcga agctgctcgt atcgccatga
    77341 ctcgttacat gaaacgtggt ggtaaagttt ggattaaaat cttcccacac aaatcataca
    77401 ctgctaaagc tatcggtgtg cgtatgggat ctggtaaagg ggcacctgaa ggttgggtag
    77461 caccagttaa acgtggtaaa gtgatgttcg aaatcgctgg tgtatctgaa gagattgcac
    77521 gtgaagcgct tcgacttgct agccacaaat tgccagttaa atgtaaattc gtaaaacgtg
    77581 aagcagaata aggagaaggc atgaaactta atgaagtaaa agaatttgtt aaagaacttc
    77641 gtggtctttc tcaagaagaa ctcgcgaagc gcgaaaacga attgaaaaaa gaattgtttg
    77701 aacttcgttt ccaagctgct actggtcaat tggaacaaac agctcgcttg aaagaagtta
    77761 aaaaacaaat cgctcgcatc aaaacagttc aatctgaagc gaaataatag actagggaag
    77821 gagaaatttc aatggaacgc aataatcgta aagttcttgt tggacgtgtt gtatctgaca
    77881 aaatggacaa gacaatcaca gttgtagttg aaacaaaacg taaccaccca gtctatggta
    77941 aacgtattaa ctactctaaa aaatacaaag ctcatgatga aaacaatgtt gccaaagaag
    78001 gcgatatcgt acgtatcatg gaaactcgcc cgctttcagc tacaaaacgt ttccgtcttg
    78061 tagaagttgt tgaagaagcg gtcatcatct aatcaaacct gaaaggagaa aactgaaatg
    78121 attcaaacag aaactcgttt gaaagtcgca gacaacagcg gtgctcgcga aatcttgact
    78181 atcaaagttc ttggtggttc aggacgtaaa tttgcaaaca tcggtgatgt tatcgtggca
    78241 tctgtaaaac aagctactcc tggtggtgcg gttaaaaaag gtgacgttgt taaagcagtt
    78301 atcgttcgta ctaaatcagg tgctcgtcgt gctgatggtt catacatcaa atttgacgaa
    78361 aacgcagcag ttatcatccg tgaagacaaa actcctcgcg gaacacgtat ctttggccca
    78421 gttgcacgtg aattgcgtga aggtggcttc atgaagatcg tgtcacttgc tccagaagta
    78481 ctttaatttt taggaacaaa ctagtcccct agcttcaagc tagggtgccc ttatgggcgt
    78541 aagaaaaatc aaggagaaac ctaatgtttg taaaaaaagg cgacaaagtt cgcgtaatcg
    78601 ctggtaaaga taagggaaca gaagctgttg tccttactgc ccttccaaaa gtaaacaaag
    78661 ttatcgttga aggtgttaac attgttaaga aacaccaacg tccaactaac gagcttcctc
    78721 aaggtggtat catcgagaaa gaagcagcta tccacgtatc aaacgttcaa gttttggaca
    78781 aaaatggtgt agctggtcgt gttggataca aatttgtaga cggtaaaaaa gttcgctaca
    78841 acaaaaaatc aggcgaagtg cttgattaat cacgaaggaa aggagaagta taatggcaaa
    78901 tcgtttaaaa gaaaaatatc ttaatgaagt agttcctgct ttgacagaac aattcaacta
    78961 ctcatcagtg atggctgtgc ctaaagtaga taagattgtt ttgaacatgg gtgttggtga
    79021 agctgtatca aacgctaaaa gccttgaaaa agctgctgaa gaattggcac ttatctcagg
    79081 tcaaaaacca cttatcacta aagctaaaaa atcaatcgcc ggcttccgtc ttcgtgaagg
    79141 tgttgcgatc ggtgcaaaag ttacccttcg tggtgaacgt atgtacgaat tcttggataa
    79201 attggtatca gtttcacttc cacgtgtacg tgacttccac ggtgtcccaa caaaatcatt
    79261 tgatggacgc gggaactaca cacttggtgt gaaagaacaa ttaatcttcc cagaaatcaa
    79321 cttcgatgac gttgacaaaa ctcgtggtct tgacatcgtt atcgtaacaa ctgctaacac
    79381 tgacgaagag tcacgtgcat tgcttacagg ccttggaatg ccttttgcaa aataatatag
    79441 gaggtaaatc taatggctaa aaaatcaatg gtagctagag aggctaaacg ccaaaaaatt
    79501 gttgaccgtt atgctgaaaa acgtgctgca ttaaaggcgg caggggacta cgaaggttta
    79561 tctaaattac ctcgcaacgc ctcaccgact cgtttacata atcgttgtag ggttacgggg
    79621 cgcccacatt cagtttaccg caaatttggt ctgagtcgta tcgcttttcg cgaacttgcg
    79681 cataaaggtc aaattcctgg tgtaacaaaa gcatcttggt aatttaagat atcaagagcg
    79741 tcaaaactcc aagtaaaaat aggaaacttg acgaagaaac taaagtttct aggaaagttt
    79801 atctttttca cacagagttt agcccgggtt caattgggct tgccaatttg aacacgagct
    79861 acagctttgg caaaaaagac caatttgctt tggagcattg cttctgcatt aaattgtcta
    79921 tttttgctcg tgctgttacg ctctttgtat catgtattaa ctagcaagtg caacttgcaa
    79981 actactagta agaggagaaa aacaaaatgg ttatgactga cccaatcgca gacttcctaa
    80041 ctcgtattcg taatgctaac caagctaaac acgaagtact tgaagtacct gcatcaaaca
    80101 tcaaaaaagg gattgctgaa atccttaaac gcgaaggttt tgtaaaaaac gttgaaatca
    80161 ttgaagatga caaacaaggc gtcatccgtg tatttcttaa atacggacca aatggtgaga
    80221 aagttatcac taacttgaaa cgtgtttcta aaccaggact tcgtgtctac aaaaaacgtg
    80281 aagaccttcc aaaagttctt aacggacttg gaattgccat cctttcaact tctgaaggtt
    80341 tgcttactga taaagaagca cgccaaaaga atgttggtgg tgaggttatc gcttacgttt
    80401 ggtaaaatca agatacaaag ctcgtaaaga acaaagcaaa attaggaagt tggagaagtt
    80461 tgtttacaaa caagccaact tatctatttt gcacagttct tagagcgtgt tcagttcagc
    80521 tcttgaacta aataagtatc tgaaccccgt gaaaactggc cgttctggcc tgacaattta
    80581 acaggagaaa ataaacatgt cacgtattgg taataaagtt atcgtgttgc ctgctggtgt
    80641 tgaactcgct aacaatgaca acgttgtaac tgtaaaagga tctaaaggag aacttactcg
    80701 tgagttctca aaagatattg aaatccgtgt ggaaggtact gaaataactc ttcaccgtcc
    80761 aaacgattca aaagaaatga aaactatcca cggaactact cgtgcccttt tgaacaacat
    80821 ggttgttggt gtatcagaag gattcaagaa agaacttgaa atgcgtgggg ttggttaccg
    80881 tgcacagctt caaggatcta aacttgtttt ggctgttggt aaatctcatc cagacgaagt
    80941 tgaagctcca gaaggaatta cttttgaact tccaaaccca acaacaatcg ttgttagcgg
    81001 aatttcaaaa gaagtagttg gtcaaacagc tgcttacgta cgtagccttc gttcaccaga
    81061 accatataaa ggtaaaggta tccgttacgt tggtgaattc gttcgccgta aagaaggtaa
    81121 aacaggtaaa taatgttgag tggttgatca tcaaccacca acctattttc caactttgtg
    81181 catagcacac gatttaaaac taaagaggtg aaaactgtga tttcaaaacc agataaaaac
    81241 aaactccgcc aaaaacgcca ccgtcgcgtt cgcggaaaac tctctggaac tgctgatcgc
    81301 ccacgtttga acgtattccg ttctaataca ggcatctacg ctcaagtgat tgatgacgta
    81361 gcgggtgtaa cgctcgcaag tgcttcaact cttgataaag aagtttcaaa aggaactaaa
    81421 actgaacaag ccgttgctgt cggtaaactc gttgcagaac gtgcaaacgc taaaggtatt
    81481 tcagaagtgg tgttcgaccg cggtggatat ctatatcacg gacgtgtgaa agctttggct
    81541 gatgcagctc gtgaaaacgg attgaaattc taataggagg acactagaaa atggcattta
    81601 aagacaatgc agttgaatta gaagaacgcg tagttgctgt caaccgtgtt acaaaagttg
    81661 ttaaaggtgg acgtcgtctt cgtttcgcag ctcttgttgt tgttggtgac cacaatggtc
    81721 gcgtaggatt tggtactggt aaagctcaag aagttccaga agcaatccgt aaagcagtag
    81781 atgatgctaa gaaaaacttg atcgaagttc ctatggttgg aacaacaatc ccacacgaag
    81841 ttctttcaga attcggtgga gctaaagtat tgttgaaacc tgctgtagaa ggttctggag
    81901 ttgccgctgg tggtgcagtt cgtgccgttg tggaattggc aggtgtggca gatattacat
    81961 ctaaatcact tggttctaac actccaatca acattgttcg tgcaactgtt gaaggtttga
    82021 aacaattgaa acgcgctgaa gaaattgctg cccttcgtgg tatttcagtt tctgatttgg
    82081 cataagaaag gggataaaat ggctcaaatt aaaattactt tgactaagtc tccaatcgga
    82141 cgcattccat cacaacgtaa aactgttgta gcacttggac ttggcaaatt gaacagctct
    82201 gttattaaag aagataacgc tgctatccgt ggtatgatca cagcagtatc tcacttagta
    82261 acagttgaag aagtaaacta atgaagtttt aggggatgtg cactgtacca tcccctaaaa
    82321 ctagatatag tcatctatga tgacatcgta taggcgagtt gatgggggag acaacctttt
    82381 ctcccttatc ggcgctagca ttttacaaaa gaggagaaaa taaaaatgaa acttcatgaa
    82441 ttgaaacctg cagaaggttc tcgtaaagta cgtaaccgcg ttggtcgtgg tacttcatca
    82501 ggtaacggta aaacatctgg tcgtggtcaa aaaggtcaaa aagctcgtag cggtggcgga
    82561 gttcgccttg gttttgaagg tggacaaact ccattgttcc gtcgtcttcc aaaacgtgga
    82621 ttcactaaca tcaacgctaa agaatacgca attgtgaacc ttgaccaatt gaacgtcttt
    82681 gaagatggtg ctgaagtaac tccagttgtt cttatcgaag caggaattgt taaagctgaa
    82741 aagtcaggta ttaaaattct tggtaacggt gagttgacta agaaattgac tgtgaaagca
    82801 gctaaattct ctaaatcagc tgaagaagct atcactgcta aaggtggttc agtagaagtc
    82861 atctaagaga ggtgacctat gttttttaaa ttattaagag aagctcttaa agtcaagcag
    82921 gttcgatcaa aaattttatt tacaattttt atcgttttgg tctttcgtat cggaactagc
    82981 attacagttc ctggtgtgaa tgccaatagc ttgaatgctt taagtggatt atccttctta
    83041 aacatgttga gcttggtgtc ggggaatgcc ctaaaaaact tttcgatttt tgccctagga
    83101 gttagtccct atatcaccgc ttctattgtt gtccaactct tgcaaatgga tattttaccc
    83161 aagtttgtag agtggggtaa acaaggggaa gtaggtcgaa gaaaattgaa tcaagctact
    83221 cgttatattg ctctagttct cgcttttgtg caatctatcg ggattacagc tggttttaat
    83281 accttggctg gagctcaatt gattaaaact gctttaactc cacaagtttt tctgacgatt
    83341 ggtatcatct taacagctgg tagtatgatt gtcacttggt tgggtgagca aattacagat
    83401 aagggatacg gaaacggtgt ttccatgatt atctttgccg ggattgtttc ctcaattcca
    83461 gagatgattc agggcatcta tgtggactac tttgtgaacg tcccaagtag ccgtatcact
    83521 tcatctatca ttttcgtaat cattttgatt attactgtat tgttgattat ttactttaca
    83581 acttatgttc aacaagcaga atacaaaatt ccaatccaat atactaaggt tgcacaaggt
    83641 gctccatcta gctcttacct tccgttaaaa gtaaaccctg ctggagttat ccctgttatc
    83701 tttgccagtt cgattactgc agcgcctgcg gctattcttc agtttttgag tgccacaggt
    83761 catgattggg cttgggtaag ggtagcacaa gagatgttgg caactacttc tccaactggt
    83821 attgccatgt atgctttgtt gattattctc tttacattct tctatacgtt tgtacagatt
    83881 aatcctgaaa aagcagcaga gagcctacaa aagagtggtg cctatatcca tggagttcgt
    83941 cctggtaaag gtacagaaga atatatgtct aaacttcttc gtcgtcttgc aactgttggt
    84001 tccctcttcc ttggtgtgat ttccatttta ccgattgcag ctaaagatgt atttggtctt
    84061 tctgatgttg ttgcctttgg tggaacaagt ctcttgatca ttatctctac aggtatcgaa
    84121 ggaatcaagc aattggaagg ttacctattg aaacgtaagt atgttggttt catggacaga
    84181 acagaataaa agtatttact gaatcagtaa atactgaggg agtggaggtt taaactctga
    84241 catttgtaag agttggatct cccctcttct attttgtttt taaatcgggg tgaaaagact
    84301 ttttgcttct atttaaaaat aaaataagga gatcaaatca tgaatctttt gattatgggc
    84361 ttacctggtg caggtaaggg aactcaagca gcaaaaatcg tagaacaatt ccatgttgca
    84421 catatctcaa caggtgatat gttccgcgct gcaatggcaa atcaaactga aatgggtgtt
    84481 cttgctaagt catatattga caagggtgaa ttggttcctg acgaagttac aaatggaatc
    84541 gtaaaagaac gcctttcaca agatgatatt aaagaaacag gattcttatt ggatggttac
    84601 ccacgtacaa ttgaacaagc tcatgccttg gacaaaacat tggctgaact tggcattgaa
    84661 ctagaaggtg ttatcaatat tgaagtgaac cctgacagcc ttttggaacg tttgagtggg
    84721 cgtatcatcc accgcgtaac tggagaaact ttccacaagg tctttaaccc accagttgac
    84781 tataaagaag aagattacta ccaacgtgaa gatgataagc ctgagacagt aaaacgtcgt
    84841 ttggatgtta atattgctca aggagaacca atcattgctc actaccgtgc caaaggtttg
    84901 gttcatgaca tcgaaggtaa tcaagatatc aatgatgtct tctcagatat tgaaaaagta
    84961 ttgacaaatt tgaaataaag cgtttttcac acttgcaaaa atccgctaca aatgttatac
    85021 tgagatagtc tgacttataa ttgttgtctc tgtgtctaga ggcatcgaat cgaaatttat
    85081 ggaggtgctt ttgcgtggca aaagacgatg tgattgaagt tgaaggcaaa gtagttgata
    85141 caatgccgaa tgcaatgttt acggttgaac ttgaaaatgg acatcagatt ttagcaacag
    85201 tttctggtaa aattcgtaaa aactatattc gtattttagc gggagatcgt gttactgtcg
    85261 aaatgagtcc atatgacttg acacgtggac gtatcactta ccgctttaaa taatcgaaaa
    85321 acttggaggg ataagaaatg aaagtaagac catcggtcaa accaatttgc gaatactgta
    85381 aagttattcg tcgtaatggt cgtgttatgg taatttgccc agcaaatcca aaacacaaac
    85441 aacgtcaagg ataagataga aaggagaaaa catggctcgt attgctggag ttgatattcc
    85501 aaatgacaaa cgcgtagtaa tctcattgac ttatgtttat ggtatcggac ttgcaacatc
    85561 taagaaaatt ttggctgctg ctggaatctc agaagatgtt cgtgtacgtg atcttacatc
    85621 agatcaagaa gatgctatcc gtcgtgaagt ggatgcaatc aaagttgaag gtgaccttcg
    85681 tcgtgaagta aacttgaaca tcaaacgttt gatggaaatc ggttcatacc gtggtatccg
    85741 tcaccgtcgt ggacttcctg tccgtggaca aaacactaaa aacaacgccc gcactcgtaa
    85801 aggtaaagct gttgcgattg ctggtaagaa aaaataatat aggaggtaaa agtcttggct
    85861 aaaccaacac gtaaacgtcg tgtgaaaaag aatatcgaat ctggtattgc tcatattcac
    85921 gctacattta ataacactat tgttatgatt actgatgtgc atggtaatgc aattgcttgg
    85981 tcatcagctg gtgctcttgg tttcaaaggt tctcgtaaat ctacaccatt cgctgctcaa
    86041 atggcttctg aagctgctgc taaatctgca caagaacacg gtcttaaatc agttgaagtt
    86101 actgtaaaag gtccaggttc tggtcgtgag tcagctattc gtgcgcttgc tgccgctggt
    86161 cttgaagtaa cagcaattcg tgatgtgact ccagtgccac acaatggtgc tcgtcctcca
    86221 aaacgtcgcc gtgtataatc atcgcattac actgcttttc gtttaagagg gagtaactaa
    86281 atgatcgagt ttgaaaaacc aaatataaca aaaattgatg aaaataaaga ttatggcaag
    86341 tttgtaatcg aaccacttga acgtggctac ggtacaactc ttggtaactc tcttcgtcgt
    86401 gtacttctag cttctctacc aggagcagct gtgacatcta tcaacattga tggtgtgtta
    86461 catgagtttg acacagttcc aggtgttcgt gaagacgtga tgcaaatcat tctgaacatt
    86521 aaaggaattg cagtgaaatc gtacgttgaa gacgaaaaaa tcatcgaact ggatgttgaa
    86581 ggtcctgctg aagtaacagc tggtgacatt ttgacagata gcgatattga aattgtaaat
    86641 ccagatcatt atctctttac aatcggtgaa ggttcttctc taaaagcgac tatgactgtt
    86701 aacagtggtc gtggatatgt acctgctgat gaaaataaaa aggataatgc accagttgga
    86761 acacttgctg tagattctat ttatacacca gttacaaaag tcaactatca agtggaacct
    86821 gctcgtgtag gtagcaatga tggtttcgac aaattaaccc ttgaaatctt gacaaatgga
    86881 acaattattc cagaagatgc tttagggctt tcagcacgta ttttgacaga acatcttgat
    86941 ttgtttacaa atcttactga gattgctaag tcaactgaag tgatgaaaga agctgatact
    87001 gaatctgacg accgtatttt agatcgtacg attgaggaac tggacttgtc tgtgcgttca
    87061 tacaactgtt taaaacgtgc cggtatcaat actgtgcatg atttgacaga aaaatctgaa
    87121 gcagagatga tgaaagtacg aaatcttgga cgcaagagtt tggaagaagt gaaactcaaa
    87181 ctcattgatt tgggtcttgg attaaaagat aaataaagga ggaatacatg gcttaccgta
    87241 aactaggacg cactagctca caacgtaaag caatgcttcg cgatttgaca actgaccttt
    87301 tgatcaacga atcaatcgtg acaactgaag ctcgtgctaa agaaatccgt aaaactgttg
    87361 aaaaaatgat tactctaggt aaacgtggtg atttgcatgc acgtcgtcaa gcagctgctt
    87421 tcgtacgtaa tgaaatcgca tctgaaaact atgatgaagc aactgataag tacacttcta
    87481 ctacagcact tcaaaaattg ttctcagaaa tcgcacctcg ttatgctgaa cgtaacggtg
    87541 gatacactcg tatccttaaa actgaatcac gtcgtggtga tgcagcgcca atggcgatca
    87601 tcgaattagt ataaaatcat caattttgtt gagtgttatg atgatggagt cttgtgctct
    87661 tagtctagct ctggtctacc gctaggattt cggtcctagc gggaacactc atcataagtt
    87721 gggatagtag acgcttgttt acgaaattgt ttttttctta agaacaactt cgtaagcagg
    87781 cgtttttgag tattttcgtt agaattatgc tatactattt gaaaagaatc ctgtttaatg
    87841 ttaaggtttc ttattttaag aagaattgga gtttacttat gaaagccatt ataactgttg
    87901 ttggtaaaga taaatctgga attgttgcag gtgtttctgg taaaattgca gaattaggat
    87961 tgaatattga cgatatctct caaactgtct tggatgaata ttttacgatg atggctgttg
    88021 tatctagtga tgaaaagcaa gattttacct atcttcgtaa tgaatttgaa gcttttgggc
    88081 aaactttgaa tgtaaaaatc aatattcaga gtgcagcgat tttcgaagct atgtataata
    88141 tctaggaggt catcatggat attagacaag ttactgaaac catcgccatg attgaggagc
    88201 aaaacttcga tattagaacc attaccatgg ggatttctct tttggactgt atcgatccag
    88261 atatcaatcg tgctgcggag aaaatctatc aaaaaattac gacaaaggcg gctaatttag
    88321 tagctgttgg tgatgaaatt gcggctgagt tgggaattcc tatcgttaat aagcgtgtat
    88381 cggtgacacc tatttctctg attggggcag cgacagatgc gacggactac gtggttctgg
    88441 caaaagcgct tgataaggct gcgaaagaga ttggtgtgga ctttattggt ggtttttctg
    88501 ccttagtaca aaaaggttat caaaagggag atgagattct catcaattcc attcctcgcg
    88561 ctttggctga gacggataag gtctgctcgt cagtcaatat cggctcaacc aagtctggta
    88621 ttaatatgac ggctgtggca gatatgggac gaattatcaa ggaaacagca aatctttcag
    88681 atatgggagt ggccaagttg gttgtattcg ctaatgctgt tgaggacaat ccatttatgg
    88741 cgggtgcctt tcatggtgtt ggggaagcag atgttatcat caatgtcgga gtttctggtc
    88801 ctggtgttgt gaaacgtgct ttggaaaaag ttcgtggaca gagctttgat gtagtagccg
    88861 aaacagttaa gaaaactgcc tttaaaatca ctcgtatcgg tcaattggtt ggtcaaatgg
    88921 ccagtgagag actgggtgtg gagtttggta ttgtggactt gagtttggca ccaacccctg
    88981 cggttggaga ctctgtggca cgtgtccttg aggaaatggg gctagaaaca gttggcacgc
    89041 atggaacgac ggctgccttg gccctcttga acgaccaagt taaaaagggt ggagtgatgg
    89101 cctgcaacca agtcggtggt ttatctggtg cctttatccc tgtttctgag gatgaaggaa
    89161 tgattgctgc agtgcaaaat ggctctctta atttagaaaa actagaagct atgacggcta
    89221 tctgttctgt tggattggat atgattgcca tcccagaaga tacgcctgct gaaactattg
    89281 cggctatgat tgcggatgaa gcagcaatcg gtgttatcaa catgaaaaca acagctgttc
    89341 gtatcattcc caaaggaaaa gaaggcgata tgattgagtt tggtggtcta ttaggaactg
    89401 cacccgttat gaaggttaat ggggcttcgt ctgtcgactt catctctcgc ggtggacaaa
    89461 tcccagcacc aattcatagt tttaaaaatt aagaaaatag gagaaatttt aagttctatt
    89521 taagattaga cgtgtatact ataatcatta aataaagacc tcctaatatt atttgaaaca
    89581 gataacactg aattagtttg aatttgattt tcatctaata tctttattta atgaactcct
    89641 aaactttttc ataataatct ccttcaaaag tcgcctgtat gggtggcttt tattttatca
    89701 ttcatgatat aatagaagca aacggaggac ggaaaatggt aaaagtacga ttgtatttgg
    89761 tacgtcatgg caagaccatg tttaacacga ttggtcgcgc gcaaggttgg agcgatactc
    89821 ccttaactgc tgaaggtgaa cgagggattc aagagttagg aatcggtttg cgagaatctg
    89881 atctacagtt tgagcgtgct tattcgagtg attctggtcg taccattcag accatgggaa
    89941 ttatccttga agaacttggc ttgcaggggg aaatccctta tcgcatggac aagcgtatca
    90001 gagaatggtg tttcggtagt tttgatggag cctatgatgg cgatcttttc atgggcatta
    90061 ttcctcgtat ctttaatgtg gaccacgttc accaattgtc ttatgctgaa ctggctgagg
    90121 gcttggtaga ggtcgataca gctggttggg ctgaaggctg ggaaaaactc agtggccgaa
    90181 tcaaggaagg ctttgaaatg attgcaaaag aaatggaaga tcaaggtgga ggtaacgccc
    90241 ttgttgtcag ccatggaatg actattggaa ccattgttta tctgattaat ggcatgcatc
    90301 cgcatggtct ggataatggt agcgtgacaa tccttgaata tgaggacggc cagtttaggg
    90361 ttgaagttgt cggtgaccgt agttaccgag agctaggacg tgagaagatg gaagaaggct
    90421 ctatttaatc agtctagact tgcttgccat gagctaggga tttgataaga atatcaagat
    90481 aagaaaaaac agccgagggc actcctttcg gctgtttttg atgtggaaaa ctaaagtgta
    90541 atgctattgc ttttagagat tttcataaac aagagcaagg aacctactgt tagaacagtc
    90601 aggatagttg acaaggttgc ggctacaccg taatttcctc tgagaacctc tgtataaata
    90661 gctacagtca ttgttcttgt tttgacattg tagaggagga tagaagtaga gagttttgaa
    90721 atcattgtga ctcaagataa gatggctcca gaaatgatac cagatagcat cattggagtt
    90781 gtaatcttag caaaggtatt gagacgacta cttcctaagc tttcagcagc ttcttcaata
    90841 cttggtgcta tttgttgtaa gctagcaaca gatgagcgaa tagtataagg taatcttctg
    90901 gcagatagag acataatcaa gatgaaagca gtccctgtaa tcataagaaa tccacttcca
    90961 aatagaccag tattgaagga agaaatgaag gcaatcccta gaacggttcc tggtacaata
    91021 taaggtacca tactgaggct gtcaattaag tttgtaaaca aattccgttt tctaacggct
    91081 aggtaggaga taaatgtcgc aaatagaaca actagaacta aggcaatcaa agggatacga
    91141 atggtattga aaatagcaga tcccatacga tggaaagcta ccttgtaact gtttggagaa
    91201 taacctttaa cagataccat acctgatgtt tttaggaaag aggtataaat taagtagatt
    91261 tgaggtaaaa cagagataaa gataattccg tagactgttg cataaatggc agccattttt
    91321 ccttttgtag tttttttagg ctcaattgga tggagcagat tcatgctgaa actgtagcgg
    91381 tttgcaatgt gtttttggat aaggaaaatt gccaaggcaa tgataatcgc cataattgca
    91441 aaagcagaat ttcctccaac ctcgctaata aattgggtat aaatcaggac agggaaagtc
    91501 cgataccctt cgccaatcaa cataggcgtt ccaaagtctg agaatgctct cataaataca
    91561 agcaaggagc tgctagtaag gttggaacta ggagaggtaa aacaaccgtt acgataggtt
    91621 taaatccgaa ggaccccatg ctttcagctg cttcaagtag agaattgtca atactgttca
    91681 ttgttccagc aacatataga aataccagtg ggaatagttg cagtgtaaag acaagtacaa
    91741 ttcctttgaa tcaataaata tcgatagctg gaagataaag ggcatttgtc aaaaatttag
    91801 tgatgacctc atttcgtcct agcaagagaa cccaggagta ggctcctacg aaaggagctg
    91861 acatggaagc aatgataatc aatatttgta gaaatttctt ccccttgaag tcatacatag
    91921 agaagagata agctaatagg gttcctacaa ctaaggaagt gatagtagcg gtaatggaaa
    91981 ccttgaaact gttgactagt gtctcagagt agtaggcttt actaaagaaa gtgacaaaat
    92041 tagctagtga gaattgtcct tcatgtataa gtgcttgctt gagcacggta acgataggat
    92101 aaacgagaaa gataggatag gtaagaaaga ggaagaaaga ggaaactgtc caaatattta
    92161 gttttttacg ttccatggtt gactcctttt atcaggtttt gggaaccatc tgcagaaaag
    92221 atgtttaatt tttgcgtatt gattcgtaga cgaatacgat tgcctttttg tagatcttct
    92281 tcaaaagttg attcttcact aacttgaatt tttgaggcaa aacctgtctc aatgaaataa
    92341 tccgtattta gtccaagata gacgctatct ctaatagttc cttcaatatc tccagattca
    92401 tctttgataa actcttcggg acgaatgctt acatgaatag cttgctcctc aacctgatca
    92461 agagctggca ttcgaagggc atagccatct gaaaagacga tataagcgcc gtcgctccgt
    92521 ttttcaagat tggcagggat aatatttgtg cgtccgataa aggttgccac aaactcatta
    92581 gctggtttat gatagagttc ttttggtcgg ccgatttgtt ggatcacccc atctttcata
    92641 acagcaattt ggtctgaaat agccatggct tcttcttggt cgtgggttac ataaacagtt
    92701 gtaattccca cttcgtgttg gatttctcgg atggcttgac gcatatccaa gcgaagtttg
    92761 gcctccagat tactaagtgg ctcgtccatg aggagaacac ttggattaac cgctaaggcg
    92821 catgccaagg tgacacgttg ttgttgtcca ccactgagtt tatcgggctt tcgatccgca
    92881 tattgagcaa tttgcatgag ttcaagatac ttgttggtct gttgaatcaa ttcttctttt
    92941 ggaaccttct tttgcataag accaaaagca acgttgtctc ggacagtcaa atgtgggaaa
    93001 atagcgtagt tttggaaaac catcccgata ttgcgtttgc tgggttccat attattgatt
    93061 tttgtatcat cgaagtaaaa ttctccacct tcgatactgt tgaaacctgc aatcatacga
    93121 agaagggtcg ttttcccaca tcctgaagct ccaagaaggg taaagagact tccttttgga
    93181 attgtaatgt tcaaattctc aataacaggg acatcgtggt agattttttt ggcgttaata
    93241 attttgatct cactcatagt gaacctcttt tactgtttag attggatatc tgtaaagact
    93301 tcgttgtatt tcttaacgat atctgattta ttcttgatga cataatcata atcttcagtg
    93361 agtgttttga ttttgtcaat tggtttcatg ttttcgcttg ttttagcatt tttacgaaca
    93421 ggacggttag tagtggttgt accaagtgta tcttgtactt cttgagagat aataaaatcg
    93481 ataaatttct tggcattttc catattttta gattttttaa cgatagcagc actagcaggt
    93541 aggaagacgg ttccttcttt tggatagact accttaatgt tagctccgtc atttaagagt
    93601 ttaactgctg gatcttcata agagagacca acagccattt ctccatcagc gactacttta
    93661 tagacactag atgaacttga accgatttta ccatcaataa gtgtgaaaag atcttttaca
    93721 taagaccaag ccttatcatc tttgtaacca ccttgagctt gtagcatatt tgttaattga
    93781 gcaaaggcgc tagaagagtt tgctgggtca gcagttgcga tttttccttt tagttcaggt
    93841 ttgaaaagat cgttatatcc ttcgatgttc atgcctttag ttaaatcagg gttgacgatt
    93901 aaaacactac catctagtgt ataaggagta gagtagccag ttgtgttttg atattctttg
    93961 ataacattat cattttcttt tgaagtatag ttttcaaaga gttctccgtg ggtagtatat
    94021 tgtgtataag aaccaccaaa gataacatca gctacaggaa cttctttttc tgactctagt
    94081 tttttgaaaa gttctccagt accagcttga atcagttcta ctttgatacc atatttttct
    94141 tcaaaggcag gaatagttgc tccaattaag ccctctgagt ttggtgaata aacgactagc
    94201 gaaccgccgt ctcctttatc agatgaactg tcatcggcag attcattaga agaacaagca
    94261 gcataataca tccatttctt tttcatgatg gatacctccg ttgtgttatt taagtttatt
    94321 ttaaaacaat gtaagcgttt ttaaaacata caattctatt ctatagtgta ttgaatttat
    94381 aacagtacac tttgactgct aaaatatttc tataaattaa tttgactttc ctgatagaga
    94441 tgttcacatc ttatttcaat tcactatatt agagtaaaat tctctacaaa aagaagaata
    94501 gcctatttta ctattcttct gagtgatttc aattcctttg gggaaatatg gagatacttt
    94561 ttaaatcctg acaaatggtt gtttcttttt ctaaatcggt gatactgtat cggagaatgc
    94621 gcgtgaggtc acaaaggctg cgatagagct tctatggaga atttcttttt ggagagattt
    94681 tttaaaggaa tgagacatcc gctacctcct tggaaggttt ttttgtgata agtatagcag
    94741 tttaaaaatt ctagagcaag gtaaaataga aaaggtttgg ctaaaatttt ttagctaaac
    94801 cttttgaaaa gttaagtatg tattatagat gcttgttatc tgcaaatagc tagagcattt
    94861 caaggaaata aactagaaat aacaattaat atggtggttc agaaatacct tttgataatc
    94921 aataagatct tcaggatgaa gtgcgttgat tccatccatt tcatatttcc gatttaataa
    94981 acgatatttg ttttcaccaa attgatgaaa agggagtagt tgaacttggt cgatatttaa
    95041 tgagttaaat agagtagcga atttttctgc atcctctaaa ctattgttaa aattaggaat
    95101 aactgggatt cttaaaacga tagttttatt ttgtgaaaaa gcataatgaa tgtttttaat
    95161 aatcatttga ttaaaaaccc cagtcacttt tttatgtttt atagaattat aatgttttag
    95221 gtctgtgtag ataaaatcca catattgaat taaatcaata aatttttcat gatcaacaaa
    95281 ggcagtagtt tcaatggcag tgtgtatgtg atgttcttta gctgatttta agatggcttt
    95341 agcaaattca aactgagcaa atatttcacc tcctgataaa gttaaacctc caccggattc
    95401 ttcgtaaaat tctttgtctt ttaatacctc tgtaataatt tcttctacag tcttttcttc
    95461 accgactaag gtgaatttct ctcgttgagc atctttcatt ttttcaggtt tcattctttg
    95521 agattcagga ttagaacacc atggacagcg cagaggacat ccttttaaaa aaacagttgt
    95581 acgaataccc ggaccgtcat gaattgaaaa gtgttgaata ttaaaaataa ttcctttaga
    95641 tatttccata agagttcctc ctcttcataa gtctatccta tcacaaaaac gaaagaaaaa
    95701 caattacgaa cgaaaggtta atttgttgtt ttttgctttt tattttggta gaataaaacg
    95761 gaggtgtaaa atgaaaagat tggaacaaat tattaaatta gtatcagaat atgaaaagat
    95821 cgacgttaat acattatcgg aaaaattaaa tgtatcgaaa gtaacgatta gaaaagattt
    95881 agataaatta gagtcaaaag gtttattaca cagagagcat ggatatgctg tattaaatag
    95941 tggagatgac ttaaatgtac gtttgtcaat taattatgaa actaagagaa aaattgttca
    96001 ggaagcagta aaattggtgt cagataatga aacaataatg atagaatctg gatcgacctg
    96061 tgctttactt gctgaggaaa tttgcaagca aaaaagaaat gttacgattg taacaaattc
    96121 gttttttata gcaaattttg tgagagctta tgattcatgt cgtgttattg ttcttggtgg
    96181 tgagtttcag aaagattcac aggtgactgt aggaccttta ttaaaagaaa tgatacagac
    96241 ttttcatgtg tgtcaagctt ttgttgggac agatggttac gataaagaga tgggctttac
    96301 cggaaaagat ttaatgcgca gtgaggtagt tcaatatatt tcagcagtgt cggataaagt
    96361 cattgtccta actgactcaa gtaaatttga taaaagaggt acagtaagaa gatttgcttt
    96421 aagtcaagtc tatgaagtaa taacagacga aaaactttct aaacaaaata tagctacatt
    96481 agaaaatgct gggataatgg ttaaggtagt ttcgtaagag gttaagtgta tgaatcaaga
    96541 taggaataaa ctgctttcta aaattgctta tctgtattat attgaaaact taaatcagtc
    96601 acaaatagca gcaaaattag gaatttatag aacctctatt agtagaatgt taacagaagc
    96661 aaggaatgta ggaattgtta aaattgaaat agagaatttt gataccaata tgtttaagtt
    96721 ggaaaattat gtaaaagaaa aatacagttt ggaaagttta gaaattattc caaatgaatt
    96781 tgatgatact ccaacaattt tatctgaaag aatttctcaa gttgcagcag gcgtccttag
    96841 gaatctaatt gatgataata tgaaaattgg cttttcttgg gggaaaagtt taagtaattt
    96901 agtagattta attcacagta aaagtgtccg aaatgttcac ttctatcctc tagcaggtgg
    96961 tcctagtcac atacacgcta aataccatgt gaatacactg atttatgaaa tgtctagaaa
    97021 atttcatgga gagtgtacat ttatgaatgc aacgattgtg caagaaaata aattgttagc
    97081 agatggtatt ttgcaatcaa gatattttga aaatttgaaa aatagttgga aagatttaga
    97141 tatagctgta gtcggaattg gtgattttag caataaaggg aaacatcaat ggttagacat
    97201 gcttacagag gatgacttta aagaattgac caaagttaaa actgtaggag aaatttgttg
    97261 tcgatttttt gattcaaaag gtaaagaagt ctatgaaaac ttacaggaaa gaacgatagc
    97321 aatctctttg gaagatttaa aaaatattcc tcaaagttta gctgttgctt acggtgatac
    97381 gaaagtatct tcgattcttt ctgtcttgcg tgctaattta gtaaatcatt tgattacaga
    97441 caaaaataca attttaaaag ttttggaaga agatggggat ttgactttta gagagattct
    97501 aggtgagtga aaatgataga ctgattcagt ttatcgtttt tctttttagt tgattgcaca
    97561 tttgtgctta tataaacaaa aatagtttat ctgttgtttt tggattgaca actttattat
    97621 gtagttgtat tctatagtta caaaagaaaa ttttaaaatt tcaaatgaaa aaagcttttt
    97681 acatagtgaa atgaggagga atttatggaa atgattgttc cagatcaaat tatcatgggt
    97741 ttaattttat atgctggtga tgcgaaacaa catatttata aagcgttaga ttacataaaa
    97801 aatggtacat gtgaacggtg tgaagaagaa atacagttag ctgatgcagc cttattagaa
    97861 gctcataatc tacaaacaaa atttttggca caggaagcgt ctggtacaaa gacagaaatt
    97921 acagctctct ttgttcattc acaagatcat ctcatgacca gtatgacgga gattaattta
    97981 atcaaagaaa ttattagttt gagaaaagaa cttcataaaa aataatacta gagtattatc
    98041 attgttatta acatagagga ggaaaacata atggtgaaga ttggtttgtt ttgtgcagca
    98101 ggtttttcta ctggtatgct tgtaaataat atgaaaattg cagcgcaatc tagtggagtt
    98161 gaggcagaaa tagaggcgtt ttctcagtct aaattagcgg attatgcgcc aaatatagat
    98221 gttgcactat tgggtccaca agttgcttat acattagata aatcaaaaga aatttgtgat
    98281 aagtgtgatg ttccgatagc tgttattccg atgatggact atggtatgtt agatgggaaa
    98341 aaagtattag atttggccct atctttgatt agtgggtaag aaaaggagat ttattatgtc
    98401 aaagatggat gttcagaaaa tcattgcacc gatgatgaag tttgtgaata tgcgtggcat
    98461 tatagctcta aaagatggga tgttagcaat tttgccattg acagtagttg gtagtttgtt
    98521 cttgattatg ggacaattgc cgttcgaagg attaaataag agcattgcta gtgtttttgg
    98581 agctaattgg acagagccgt ttatgcaagt atattcagga acttttgcta ttatgggtct
    98641 aatttcttgt ttttcaattg cctattctta tgctaagaat agcggagtag aggctttacc
    98701 agctggagtt ctatctgtat ctgcattctt tattttgcta agatcatctt atatccctaa
    98761 acaaggtgag gcgattgggg acgctattag taaagtttgg tttggaggcc aaggaattat
    98821 cggtgctatc attataggtt tggtagtagg aagtatttat accttcttta taaagagaaa
    98881 aattgttatt aagatgccag aacaagttcc acaagctatt gccaaacagt ttgaagcaat
    98941 gattccagca tttgtaattt tcttatcttc tatgattgta tatattttag cgaagtcatt
    99001 gactaatggc ggaacattca tagaaatgat ttattctgct attcaagttc cgttgcaagg
    99061 tttaactgga tctttgtatg gtgctattgg aattgcattc tttatatcat ttttgtggtg
    99121 gtttggtgtt catgggcaat cggtagtaaa tggagtagtg acagctctgc ttttatctaa
    99181 tcttgatgct aataaagcta tgttagcctc tgctaatcta tcattagaaa atggtgcaca
    99241 tattgttact caacaatttt tagattcatt tttaattcta tcaggttcag ggattacgtt
    99301 tggtcttgta gttgccatgc tttttgcagc aaaatcaaaa caataccaag ccttaggaaa
    99361 agttgcagct tttccagcaa tatttaacgt aaatgagcca gttgtatttg gatttccgat
    99421 tgtcatgaat ccagttatgt ttgtaccttt cattcttgtt cctgtacttg cagctgtgat
    99481 agtatatgga gctattgcaa caggtttcat gcagccattc tcaggggtaa cattgccttg
    99541 gagtacacca gctattttat caggattttt ggtgggtgga tggcaaggag ttattactca
    99601 gctggtgata ttagcgatgt ctacattggt ttattttcca ttctttaaag tacaggatcg
    99661 tttagcttac caaaatgaaa tcaaacaatc ttagaggtat ttgtgtgtta ctgttaaact
    99721 cacacatttg tgctaaaaat tagagagtta aaatttttct agttaaaagc ttgaaaattt
    99781 ctataaaaat cggtattata ttttcgaaag aaataaaaat attttcgaaa gaaaggtgct
    99841 tacgatggta aatacagaag tagcaagaac aacaatcaag acagaatatt ttggcagcct
    99901 tactgaaagg atgaacaaat atcgagaaga tgttttaaat aaaaaacctt atattgatgc
    99961 tgagagagca gttctagcaa cacgcgccta tgaacgatac aaggaacaac ctaatgtcct
   100021 aaaacgtgca tatatgctga aagaaatttt ggaaaatatg actatctata ttgaagaaga
   100081 atctatgatt gcgggaaatc aagcttcttc caataaagat gctcctattt ttccggaata
   100141 tacgctagaa tttgttctca atgagttgga tctttttgaa aagcgtgatg gagatgtttt
   100201 ctatattaca gaagaaacaa aagaacaact tagaagtatt gctccgtttt gggaaaataa
   100261 taatttacgt gctagagctg gtgccttatt acctgaagaa gtgtctgttt atatggaaac
   100321 aggattcttc ggtatggaag gtaagatgaa ttctggagat gctcacttag cagttaacta
   100381 tcagaaactt ttgcaatttg gtttaagagg ttttgaagag cgggctcgta aagcaaaagt
   100441 agctctagat ttaacagatc cagcaagtat tgataaatat catttttacg actctatatt
   100501 tatcgtaatc gatgctatta aagtatatgc aaagcgcttt gttgctcttg ctaaaagttt
   100561 agccgaaaat gcaaatccta aacgtaagaa agaattactt gagattgcag atatttgctc
   100621 tagagtccca tatgaaccgg caactacttt tgcagaagct attcaatcag tttggtttat
   100681 tcaatgtatt ttacaaattg aatctaatgg ccactctctt tcatatggcc gttttgatca
   100741 atatatgtat ccatatatga aggctgattt agaaagtggt aaagaaacag aagatagcat
   100801 tgttgaacgt ctgacaaatc tttggattaa gacaattaca attaataagg ttcgcagtca
   100861 atcacataca ttttcttcag caggaagtcc tttatatcaa aatgttacaa ttggtggaca
   100921 gactcgagat aagaaggatg ctgttaaccc attatcttat ttggtattaa aatcagttgc
   100981 acaaacccat ctaccgcaac ctaatctaac tgtacgttac catgcaggtt tagatgctcg
   101041 tttcatgaat gagtgtattg aagtgatgaa acttggtttt ggtatgcctg catttaataa
   101101 tgatgagatt attattcctt cttttattgc aaaaggagta ttggaagatg atgcttatga
   101161 ttacagtgcc attggatgtg ttgaaacggc agttccaggg aaatggggct atcgttgcac
   101221 aggtatgagt tatatgaact tccctaaggt tctacttatc acgatgaatg atggaattga
   101281 tccggcttcg ggtaaacggt ttgcaccaag ctttggtcgt tttaaggata tgaagaactt
   101341 ttctgaatta gaaaatgctt gggataaaac actaagatat ttgacacgaa tgagtgttat
   101401 tgttgaaaat tctattgatt tatcattgga acgagaagtt cctgatattc tatgttcagc
   101461 attgactgat gattgtattg gtcgtggaaa acaccttaaa gaaggtggag cagtatatga
   101521 ttatatatca ggattgcaag ttggaattgc aaatttgtcg gattcattag ctgcaattaa
   101581 aaaattggtg tttgaggaag aacgtataag cccaagtcag ctttggcatg cactggaaac
   101641 agattatgcc ggagaagaag gtaaggtcat tcaagaaatg ttgattcatg atgcacctaa
   101701 gtatggtaat gatgatgatt atgctgacaa attggttact gctgcttatg acatttatgt
   101761 tgatgaaatt gctaaatatc ctaatacacg ttatggaaga gggcctattg gaggaattcg
   101821 ttattcagga acatcttcta tctcagccaa cgtagggcag ggacgtggaa cattagcaac
   101881 tccagatgga cgcaacgcgg gtacaccgtt agcagagggt tgttcaccat cacataatat
   101941 ggatcaacac ggccctacat ctgttttaaa atctgtttca aaattaccaa cagatgaaat
   102001 cgtaggtggg gttctcttaa atcagaaagt aaatcctcaa acgttagcca aagaagaaga
   102061 taaattaaaa ctaattgctt tgttacgaac attctttaat cgtttacatg ggtaccatat
   102121 tcaatacaat gttgtttcca gagagacgct gattgacgct cagaaacatc ctgaaaaaca
   102181 cagagactta attgttcgtg ttgcaggata ctctgcattc ttcaatgttc tttctaaggc
   102241 aacccaagat gacattatag gacgtactga gcatactttg taaaataaag aggttctttt
   102301 tatggaattt atgcttgaca cattaaattt agatgagatt aaaaagtggt ctgaaatttt
   102361 gccgctagct ggggtaactt caaatcccac tattgcaaaa agagagggtt ctattaattt
   102421 ttttgaacga atcaaagatg taagagaatt gattggctct acaccctcta ttcatgttca
   102481 ggtgatttct caagattttg aaggcatctt aaaggatgct cataaaattc gaagacaagc
   102541 aggagatgat atatttatca aagtacctgt tactccagct ggattacgtg caataaaggc
   102601 gctaaaaaaa gagggctacc atatcactgc agcagctatt tatacagtta ttcagggatt
   102661 attagctatc gaagcaggag cggattacct agctccatat tataatagaa tggaaaatct
   102721 gaacattgat tcaaattctg tcattcgtca attagctctt gctattgata gacagaactc
   102781 tcctagtaag attttagctg catcctttaa aaatgtagca caagtaaata atgctttagc
   102841 tgcaggtgcg catgctgtta cagcaggagc ggatgttttt gaatcagctt tcgccatgcc
   102901 atctatccaa aaggcggttg atgatttttc tgacgattgg tttgttattc aaaatagtcg
   102961 ttccatttag atagagagga aatacatatg agaatttttg ctagtccttc tagatatatt
   103021 cagggggaaa atgccttgtt tgaaaatgcc aaatcaattt tggatttggg aaattgccct
   103081 attctattat gcgatcagtt ggtttatgat attgttggaa aacgatttga agattaccta
   103141 cataggtatg gtttccatat tgttctggcg ctatttaatg gtgaagcttc tgacaatgaa
   103201 atcaatcgag ttgttgcctt ggctgagaaa gaaaattgtg atagtattat cggtcttggt
   103261 gggggaaaga cgattgatag cgcaaaagct attgcagatt tgattgaaaa gcctgttatt
   103321 attgctccaa caattgcatc gaccgacgca cctgtatctg ctttatctgt tatttataca
   103381 gatgaaggtg catttgatca ttatctattt tattctaaaa atccagattt agttttggtt
   103441 gatacaaaag ttatttcaca agcccctaag cgtttattag cgtctggtat tgcagatggt
   103501 ttagcaactt gggttgaggc gcgtgcggtt atgcaggcaa atggaaaaac tatgttggga
   103561 caacagcaaa cattggctgg agttgcaatt gcgaagaaat gtgaagaaac gctgtttgca
   103621 gatggtttac aggctatggc agcttgtgaa gctaaagtgg tgacaccagc attagaaaat
   103681 attgttgaag ctaatacttt attgagtggt ctaggttttg aaagtggagg attagctgcg
   103741 gcgcatgcaa ttcataatgg ttttactgca ttgacaggtg acattcatca tttaacacat
   103801 ggtgaaaaag tagcttatgg aactttagta caactattat tggaaaatag acctaaagaa
   103861 gaacttgata agtatattga gttttacaaa aaaattggta tgccaacaac tctaaaagaa
   103921 atgcatttgg atcaagttgg atatgatgat ttaataaaag ttggtaaaca agcaactatg
   103981 gagggtgaga caattcatca gatgccgttt aagatttcgc cttcagatgt tgctcaagct
   104041 attatcgctg tagatgccta tgtaaattca aaataaacaa taaggactac tgttttccaa
   104101 atggtagtct tttattgatc cctgtattga attctataga agattgaaat aggatgagaa
   104161 caaatcgatt gggaaagtaa aattaatttc tataaatgtt ttagcaattg tttcgtacta
   104221 tttcagattc agtctactat atgttcttca taaatcaaaa agcgacatag gttgtcggct
   104281 atttattgtg aatacattaa ttagcattcc agttttatct tcggtctaaa ataagtattt
   104341 tgtgctatac gagataagct tcttgactta ctccttgatt tactgcataa caatgggata
   104401 aaaagtggga gatagagcaa ttcatagtca tcaaaattaa tgagatacag tatacagttt
   104461 ttcctttaaa cacatttcaa attccctcaa aaatggtata atagtaacat cacaaaattg
   104521 gagagagacc atgagttttt acaatcataa agaaattgag cctaagtggc agggctactg
   104581 ggcagaacat catacattta agacaggaac agatacatca aaacctaagt tttatgcgct
   104641 tgatatgttc ccttatccgt ctggagctgg tctgcacgta ggacacccag aaggttatac
   104701 tgcaaccgat atcctcagtc gttacaaacg tgcgcaaggc tacaatgtcc ttcacccaat
   104761 gggttgggat gcttttggtt tgcctgcaga gcaatacgct atggatactg gtaatgaccc
   104821 agcagaattt acagcggaaa acattgccaa cttcaaacgt caaattaatg cgcttggatt
   104881 ttcttatgac tgggatcgtg aagtcaacac aacagatcca aactactaca agtggactca
   104941 atggattttc accaagcttt acgaaaaagg cttggcctat gaagctgaag tgccagtaaa
   105001 ctgggttg
//
LOCUS       DBDESG010000002        58343 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.002, whole genome shotgun sequence.
ACCESSION   DBDESG010000002 DBDESG010000000
VERSION     DBDESG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 58343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.002"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(685..1686)
                     /locus_tag="ACKB38_000127"
     CDS             complement(685..1686)
                     /locus_tag="ACKB38_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7978982.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(1794..2162)
                     /locus_tag="ACKB38_000128"
     CDS             complement(1794..2162)
                     /locus_tag="ACKB38_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978983.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(2258..2968)
                     /locus_tag="ACKB38_000129"
     CDS             complement(2258..2968)
                     /locus_tag="ACKB38_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGL7978984.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(2971..4662)
                     /locus_tag="ACKB38_000130"
     CDS             complement(2971..4662)
                     /locus_tag="ACKB38_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7978985.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(4674..5684)
                     /locus_tag="ACKB38_000131"
     CDS             complement(4674..5684)
                     /locus_tag="ACKB38_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7978986.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(5787..6854)
                     /locus_tag="ACKB38_000132"
     CDS             complement(5787..6854)
                     /locus_tag="ACKB38_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGL7978987.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            complement(6879..7538)
                     /locus_tag="ACKB38_000133"
     CDS             complement(6879..7538)
                     /locus_tag="ACKB38_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGL7978988.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            complement(7722..8732)
                     /gene="galE"
                     /locus_tag="ACKB38_000134"
     CDS             complement(7722..8732)
                     /gene="galE"
                     /locus_tag="ACKB38_000134"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGL7978989.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            complement(8745..10220)
                     /gene="galT"
                     /locus_tag="ACKB38_000135"
     CDS             complement(8745..10220)
                     /gene="galT"
                     /locus_tag="ACKB38_000135"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGL7978990.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            complement(10233..10874)
                     /locus_tag="ACKB38_000136"
     CDS             complement(10233..10874)
                     /locus_tag="ACKB38_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGL7978991.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERVELI
                     KEDLIINESVLNIDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QILQNNEILKKIISKIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSI
                     NMEKKIMNYIFEDDGNVSEVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            complement(10932..11315)
                     /locus_tag="ACKB38_000137"
     CDS             complement(10932..11315)
                     /locus_tag="ACKB38_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978992.1"
                     /translation="MLVNEMEITDHRVDNLFEKGKNEIKDSIGTNSVLNKKIILQKIR
                     KLSNQPSGYWIGSLDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELIK
                     MRFNSSAIDPIYGKKDDASILVIDV"
     gene            complement(11475..11771)
                     /locus_tag="ACKB38_000138"
     CDS             complement(11475..11771)
                     /locus_tag="ACKB38_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7978993.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
     gene            complement(11755..13914)
                     /gene="pfbA"
                     /locus_tag="ACKB38_000139"
     CDS             complement(11755..13914)
                     /gene="pfbA"
                     /locus_tag="ACKB38_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGL7978994.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKIQNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKRLKYYGKDK"
     gene            complement(15044..16270)
                     /locus_tag="ACKB38_000140"
     CDS             complement(15044..16270)
                     /locus_tag="ACKB38_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGL7978995.1"
                     /translation="MPNYNIPFSPPDITEAEITEVVDTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSIYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(16313..17005)
                     /locus_tag="ACKB38_000141"
     CDS             complement(16313..17005)
                     /locus_tag="ACKB38_000141"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGL7978996.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(17178..18929)
                     /locus_tag="ACKB38_000142"
     CDS             complement(17178..18929)
                     /locus_tag="ACKB38_000142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7978997.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQETSDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(18919..20664)
                     /locus_tag="ACKB38_000143"
     CDS             complement(18919..20664)
                     /locus_tag="ACKB38_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HGL7978998.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLLAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKEMRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(20870..21247)
                     /locus_tag="ACKB38_000144"
     CDS             complement(20870..21247)
                     /locus_tag="ACKB38_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGL7978999.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(21319..21775)
                     /locus_tag="ACKB38_000145"
                     /pseudo
     CDS             complement(21319..21775)
                     /locus_tag="ACKB38_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            21909..22736
                     /locus_tag="ACKB38_000146"
     CDS             21909..22736
                     /locus_tag="ACKB38_000146"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGL7979000.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLKIDL"
     gene            22748..22891
                     /locus_tag="ACKB38_000147"
     CDS             22748..22891
                     /locus_tag="ACKB38_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGL7979001.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            23002..23130
                     /locus_tag="ACKB38_000148"
     CDS             23002..23130
                     /locus_tag="ACKB38_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGL7979002.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      23513..23608
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23999..24580
                     /locus_tag="ACKB38_000149"
     CDS             23999..24580
                     /locus_tag="ACKB38_000149"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGL7979003.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAEKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDCFENGQVVFKEADL"
     gene            24580..25842
                     /locus_tag="ACKB38_000150"
     CDS             24580..25842
                     /locus_tag="ACKB38_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGL7979004.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(25892..26353)
                     /locus_tag="ACKB38_000151"
     CDS             complement(25892..26353)
                     /locus_tag="ACKB38_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HGL7979005.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(26350..27114)
                     /locus_tag="ACKB38_000152"
     CDS             complement(26350..27114)
                     /locus_tag="ACKB38_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HGL7979006.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(27295..27699)
                     /locus_tag="ACKB38_000153"
     CDS             complement(27295..27699)
                     /locus_tag="ACKB38_000153"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGL7979007.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(27769..29250)
                     /locus_tag="ACKB38_000154"
     CDS             complement(27769..29250)
                     /locus_tag="ACKB38_000154"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGL7979008.1"
                     /translation="MTLVNKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGAELMDLVTPCPSQVNRDFWATYAHSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(29269..30447)
                     /locus_tag="ACKB38_000155"
     CDS             complement(29269..30447)
                     /locus_tag="ACKB38_000155"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGL7979009.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            30612..31610
                     /gene="galR"
                     /locus_tag="ACKB38_000156"
     CDS             30612..31610
                     /gene="galR"
                     /locus_tag="ACKB38_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGL7979010.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALQEAGISLPDRVSLISFNDTSL
                     TKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQE"
     gene            complement(31656..32693)
                     /locus_tag="ACKB38_000157"
     CDS             complement(31656..32693)
                     /locus_tag="ACKB38_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGL7979011.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(32694..33047)
                     /gene="nmlR"
                     /locus_tag="ACKB38_000158"
     CDS             complement(32694..33047)
                     /gene="nmlR"
                     /locus_tag="ACKB38_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGL7979012.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(33291..34181)
                     /gene="czcD"
                     /locus_tag="ACKB38_000159"
     CDS             complement(33291..34181)
                     /gene="czcD"
                     /locus_tag="ACKB38_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGL7979013.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            34314..34850
                     /gene="sczA"
                     /locus_tag="ACKB38_000160"
     CDS             34314..34850
                     /gene="sczA"
                     /locus_tag="ACKB38_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGL7979014.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(35017..35823)
                     /gene="pnuC"
                     /locus_tag="ACKB38_000161"
     CDS             complement(35017..35823)
                     /gene="pnuC"
                     /locus_tag="ACKB38_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGL7979015.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(36168..37688)
                     /locus_tag="ACKB38_000162"
     CDS             complement(36168..37688)
                     /locus_tag="ACKB38_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGL7979016.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATIEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(37681..38409)
                     /locus_tag="ACKB38_000163"
     CDS             complement(37681..38409)
                     /locus_tag="ACKB38_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7979017.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(38424..39188)
                     /locus_tag="ACKB38_000164"
     CDS             complement(38424..39188)
                     /locus_tag="ACKB38_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGL7979018.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(39192..39635)
                     /locus_tag="ACKB38_000165"
     CDS             complement(39192..39635)
                     /locus_tag="ACKB38_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGL7979019.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(40317..40613)
                     /locus_tag="ACKB38_000166"
     CDS             complement(40317..40613)
                     /locus_tag="ACKB38_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGL7979020.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(40635..41699)
                     /gene="pepA"
                     /locus_tag="ACKB38_000167"
     CDS             complement(40635..41699)
                     /gene="pepA"
                     /locus_tag="ACKB38_000167"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGL7979021.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            42100..42801
                     /gene="galE"
                     /locus_tag="ACKB38_000168"
     CDS             42100..42801
                     /gene="galE"
                     /locus_tag="ACKB38_000168"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGL7979022.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            42817..42927
                     /locus_tag="ACKB38_000169"
     CDS             42817..42927
                     /locus_tag="ACKB38_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGL7979023.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            43224..44183
                     /locus_tag="ACKB38_000170"
     CDS             43224..44183
                     /locus_tag="ACKB38_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979024.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTHKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            44173..45129
                     /locus_tag="ACKB38_000171"
     CDS             44173..45129
                     /locus_tag="ACKB38_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGL7979025.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSHQ"
     gene            45126..45878
                     /locus_tag="ACKB38_000172"
     CDS             45126..45878
                     /locus_tag="ACKB38_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979026.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            45974..46939
                     /locus_tag="ACKB38_000173"
     CDS             45974..46939
                     /locus_tag="ACKB38_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7979027.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELTKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(47164..47406)
                     /gene="yidD"
                     /locus_tag="ACKB38_000174"
     CDS             complement(47164..47406)
                     /gene="yidD"
                     /locus_tag="ACKB38_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGL7979028.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(47406..48128)
                     /locus_tag="ACKB38_000175"
     CDS             complement(47406..48128)
                     /locus_tag="ACKB38_000175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGL7979029.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(48115..48684)
                     /gene="scpB"
                     /locus_tag="ACKB38_000176"
     CDS             complement(48115..48684)
                     /gene="scpB"
                     /locus_tag="ACKB38_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGL7979030.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(48703..49431)
                     /locus_tag="ACKB38_000177"
     CDS             complement(48703..49431)
                     /locus_tag="ACKB38_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGL7979031.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLTFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(49431..50165)
                     /gene="xerD"
                     /locus_tag="ACKB38_000178"
     CDS             complement(49431..50165)
                     /gene="xerD"
                     /locus_tag="ACKB38_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGL7979032.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(50162..50623)
                     /gene="cbpB"
                     /locus_tag="ACKB38_000179"
     CDS             complement(50162..50623)
                     /gene="cbpB"
                     /locus_tag="ACKB38_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGL7979033.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(50620..51141)
                     /locus_tag="ACKB38_000180"
     CDS             complement(50620..51141)
                     /locus_tag="ACKB38_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGL7979034.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(51117..52088)
                     /locus_tag="ACKB38_000181"
     CDS             complement(51117..52088)
                     /locus_tag="ACKB38_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGL7979035.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSIYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(52085..52879)
                     /gene="racE"
                     /locus_tag="ACKB38_000182"
     CDS             complement(52085..52879)
                     /gene="racE"
                     /locus_tag="ACKB38_000182"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGL7979036.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(53087..53335)
                     /locus_tag="ACKB38_000183"
     CDS             complement(53087..53335)
                     /locus_tag="ACKB38_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGL7979037.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(53591..55216)
                     /gene="treC"
                     /locus_tag="ACKB38_000184"
     CDS             complement(53591..55216)
                     /gene="treC"
                     /locus_tag="ACKB38_000184"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGL7979038.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEKMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(55394..57361)
                     /gene="treP"
                     /locus_tag="ACKB38_000185"
     CDS             complement(55394..57361)
                     /gene="treP"
                     /locus_tag="ACKB38_000185"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGL7979039.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWHKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            57546..58256
                     /gene="treR"
                     /locus_tag="ACKB38_000186"
     CDS             57546..58256
                     /gene="treR"
                     /locus_tag="ACKB38_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGL7979040.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
BASE COUNT        17493 a        12885 c        10299 g        17666 t
ORIGIN      
        1 tcagttctat ctgcaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
       61 tgctctttga ttttcattga gtattactag cttttttcgt attagtccag cctttttgtt
      121 tgcttttagt agtaggcaag gagctgtaga tagaactcaa gttcatcaaa gcgacttaag
      181 gccctaataa aagataaacc aaacgacgga tagaaaaaag cccacacaca gaatatactt
      241 ccgtgtgagg gcgttggtaa cgcggtgcca cctcaattat aaagggacta tccctttaca
      301 tctctgcctt gtttaacaac aagctgcact gtaaggtgtg cgcaccgaat tttcattgtt
      361 tcaaattcat tttcaaaatc agcccacttt cactacttcc aaccacctat tcacaatcac
      421 cacaggctcc ctgaagatca aaaatagtta cttttctgat ttgttgaact tattttaata
      481 ctttgttttt tctttgtcaa gactttttta cgattttttt gaaaatatca ttcgaatgtg
      541 accatgtctt ccttagatcg aacatgaaca tgtcccactc gttagaaatt ggatccaact
      601 caatagaaac tgaatggagg ctaaacagaa cttattttag aacactccat cttttccact
      661 aggattttca agaattaaac aatactagaa actctgtctc ctaacaaatt taggagaaac
      721 ttcaacagat gtgacacttt cccctttaat aattgctaaa acaccttcta tcatttcttt
      781 agccaattta acataattgg gagcaattgt agacaaagct ggagtataat actgagaaat
      841 aggaatatta tcaaatccaa tgatagaaat atcatctgga ataagaattc ctttctcata
      901 gcacgcacga atcaagccct gaaccttttc atctcctgaa acaaaaataa tgtccggata
      961 attttgggta gtcaagtgct gcattgcata agaataaact gaatcaattg tagataagcc
     1021 ataaatgact tttaaatcca taaagtaatt tttatcattc agaaaagaac gcacacctct
     1081 ttcacgatcc ttattaacat gggattctcc tcccataagc aaccacatat ttttaaattt
     1141 ttcttcagtt acagctttca tcatatcata agtagcttga aaattattat tagatacata
     1201 gactactcca gacgtttgag attcaccgaa aacaagaaaa ggcatatggt tcttctttaa
     1261 atactgaatt ctgatatcat ctacactttc ataaaaaaca ataacaccat ctactaggct
     1321 acctgtgctt gatataattg aattactaat tgtatcctcc tctccaaagt actcaactat
     1381 agcattaaca ccaaattctt tacacgtccg taacacttta tctaacagcg tatgaaacca
     1441 aattaaagga aaagagtcga ttttttttac agaaatcaat atatttatag cttctttttt
     1501 agttaaattt tttgcatacg catttggaat atacgacaat tcctctataa ctttttgaat
     1561 cgcttgataa gtttcttctt taacatttac tccaccatta ataactcgtg aaactgtttt
     1621 tggagaaaaa cctgataaac gtgcaatatc ataaatagtt acctttttcc catttatatt
     1681 tttcatttca gtcctccatt acgaacattc taatattact atacaatatt taattttttt
     1741 taacaagaga atttagtaaa ttatttaaga tccacaaatt cacaaaatta attttacaaa
     1801 tattcttccc cttcaaaaaa gtttaaattg catttcacac ctttattttt aagaatgttt
     1861 ccaacttcac gacaaataaa ttcatatgag aaaaagctac cataaaattg tagattaact
     1921 ttttcagtaa aatgtgtagg atttataaaa acatataata gcctgtcaat gtaacatttt
     1981 aacatagagt taattttttc tttaaagata acatttgtta tcaactcatc aggaggtaaa
     2041 tgaaaggcaa acaccatttc acaaatatca taaaaagaaa taaatttgta tacttgtatc
     2101 aaacaattat tatcaaaata ttctatttta cctaaatcaa aattgatttt ataatctttc
     2161 ataaaaacct ctgagcaaaa atctactcaa aaattagatg attaaaacat ctaaaaaaca
     2221 aaaggacaaa aacatctgtc cctttgttta ctaaatttca gctaatttct tcgacataaa
     2281 taacacctac aatattagca atttcttcca tcagtcgaag atgttcaaat ctacctgata
     2341 attccagagt aataaatgac gctatttttt tgtccggaac atcaaagtat tcaattctgt
     2401 cagaattaac atctccaaac gctgttcttg aatcggtcat tctgatacca ttttctgcac
     2461 aataaaccaa tacacgatta taggcttctg tagatttaac tactatatac aattcaatca
     2521 ttttagaacg attttgcaga tattttttta gtggttggaa catggatatc acaccccaaa
     2581 cagaaatggc tactaaaaga gctccctcat aaaaacctac tccaatagct aatccaattc
     2641 ctgccgaagc ccaaatgcct gctgcagttg tcagacctgt aattttcttt ttatctgtaa
     2701 taagaatcgt tccagcgcct agaaaaccca cacctgatat aacttgagct cctaatcgtg
     2761 taggatctcc tgtcccaaat ttataagata cgtattcatt cgtcatcata atcaaacatg
     2821 cagctagaca aacaatacta taagttcgga tgcctgcagg ctgggatttg ctccctctct
     2881 ctaaaccaat tatactacca atgactactg ataaaacaat cctgacaact atttcaatat
     2941 ttgataaccc aagactagtg gctgtcatga ttatttcctt actttacgcc ccggtctttg
     3001 tgtgaagtat aataccgttc cagaaataat catcagaaca attgtataaa caaataccag
     3061 agcttgtgca ttagatgttg ctgtttcatc acctgcagat cgaatcgtaa tacctaatgg
     3121 ttgagctagg ggatggtaaa ggaatacaga taagtcgaag tcagttaata aagagttaaa
     3181 gtttaaagca ataacagaga gaacaaccgg taaaataaat ggaatgataa ccttcatcat
     3241 agtataaaaa ggtgaagcac ccatacttct tgctgcatct tccatctcat catcaacact
     3301 aaataaaata gcacgtacca ttctataaga aaatgggatt tttacaacta tatatgcaat
     3361 aagtagaatt accaaactac ctaccaaaat ctgattcaag acaagaaatt gtggctgatt
     3421 aaaagtaaat aataaactta ctgctaaaag tgtacttggt agtaaccaag gaagtagagc
     3481 accatattca aataagaaat caaaacgaga tttatgtttt ctgacaacac gagcaaatac
     3541 aactgcgaga attgttgctg ttgtcgcagc aataatagaa taaataaagc tgaccaagaa
     3601 tggagagaat gccgcactat tactaaagaa taagcgataa ttttctaaag taaagtttga
     3661 taatgttaag ttacctgttt gaattgcaac tggatctgta aatgagtata atactataaa
     3721 aattagtgga agcatgaaaa ctgtgaacaa tccatatgct acaatgtgag caatgatatt
     3781 ccaaggctta gacgcaattt tttgtttttt aagaggcgct ttagtcttag agatagaaat
     3841 ataatttcca cctttttcta tcttattcat gatagtaagc aaaattgtag ttgcaatacc
     3901 taaaataatt gcaagtaagg cagctaaatc acgagaattc cccatccctg caaatgtaat
     3961 aatcattgga tttatagttt gaaattcttt accaccaaca atcatgggtg ctgctactgc
     4021 agataaacca ctaagaaaaa ccataatagt aagtgcaaat agagttggaa ttaaggttgg
     4081 taacactact tttcggaaaa cagtaaatgg ttttgctccc atatttcgag cagcctcaat
     4141 agtgtgatag tcaacgcttc gaattgtatt tgttaaaaac aatgtatgat tagcagttcc
     4201 tgaaaatgtc ataatgaata agactgcacc atacccaata aaccagttag ggtctaaaga
     4261 agggataaca ttttgtaaaa attttgtaat caatccataa ggaccataga caaatttata
     4321 tccagtcgct aaaaccactc ctccataaat taaagaggtc atataaccta attttaaaat
     4381 tttagcacct ttaatatcaa agtactctgt aaatagaaca caaagaatac ctacgacatt
     4441 aactgtaata atgagtgaaa atgctaactt aaaactgttc ataatactct gaagtgccct
     4501 ctgagatttt agaacacgat gtacagcatc aagggaaaat tctcctcctt ttacaaatac
     4561 attcactact agatcaaagt ttggataaat aataaatgtt actaagaacc agattaaccc
     4621 taaacgaata agccaatctt ttaaatttaa tttatgacgc atactgcacc tccttaaaat
     4681 tgcagaacgt ctgatggtgt gataaataat tccacacttt ctccgacaga tctaatagca
     4741 gcctgactat caatacttgt tacattaaga atctgacttt cagaaacttt tattgtatag
     4801 tgaattgtaa ctccagaaaa ctcaacatca ataattgtcc cttttagaat aaaatcttgt
     4861 tcagtttcac gattgaatcg aactttctct aatcgaatgt atcctttttt atcctctaag
     4921 aaaacgcttg tatttttcaa taatacttcg tggactgttt catcggtcaa aacattaata
     4981 tctccaataa aatcacatac aaattcagtt tgagaattat gataaatctc tactggtgta
     5041 ccgacctgtt cgatgtatcc attgttaaag actgcaattc tatcagataa agtcaaggct
     5101 tcctcttgat catgagtaac atataaagta gtaataccta actctttttg aagtcttttc
     5161 aactcttttc tcaaatctac acgtaatttt gcgtcaaggt ttgacaatgg ttcatctaga
     5221 caaagaattt taggttcaag aaccagagca cgagccaatg ctaccctttg ttgttgaccc
     5281 ccagataatt ctgatacatt acgctgtaat tgttgatcag agatcttaat ttttgctgcc
     5341 actgctgata ctttagcttt aataacatct ggagctacct tcttaacttt taaaccaaat
     5401 gcaatattat caaaaacagt catagttgga aatagcgcat aagattgaaa tacaatacca
     5461 attccacgct tttcaggttc caaatgagtg acatctgttc cattaacttc aatacttcct
     5521 gatgatggat ctagaaaacc taccaatgct ctcaaagtag ttgatttacc acatcctgaa
     5581 ggcccaagaa atgtaaaaaa ttccccttca tgtatatcta aattcagatt atcaattgca
     5641 acaaaatcac catatttaat ttgaatatta tcaaatttaa tcatctcact aactccctct
     5701 attactaaac caaaagcctc tctttatttc ttccataaat ttagaaataa tagagagact
     5761 tggacataaa aattaactct tatttcttat tgtacgtatt ctaattcagc tttttctacc
     5821 cattcatcca aatgctttcc aacagcttcc cagtcaatat tttgtggttt cacttgatca
     5881 acaaatttct tcgtatcttc aggtagatct ttgagggcat ctttatttgc aggaatagat
     5941 ccaaagttct tactatattc tacttgaatt tctgattgac caaaccaatc aataaattct
     6001 ttagctaacg cttgtttttt actagtgctt aaaaccatag tttgttcagt tacaaatggt
     6061 acaccaatct caggagtcat aactttgaaa acaacatttt gttctttttg tccaactaat
     6121 gcaccagaac cccacatcat tccatattgt attggatctt ctttgtctaa catcttaaca
     6181 attgaacttt ctcccttttg aagagtgtat gcatttttca aatattcttt tgctacttcc
     6241 caaccttttt cggaaacacc taattcacct ttatcatcaa ggtatcgaac taagatactt
     6301 gctagaattg cccgtcctgt acctccttga agaccagaaa ttgaatattt acctttatac
     6361 ttactaccta attcagtcca atctttaggc atttctttta catcaggcgc cccaattaaa
     6421 actaatggtt gaacaatcac aggattataa taattatctt tatctgataa agattgatca
     6481 attttatcta accatttagg cttgtactgt actagtaatt tttgatctct aattttattt
     6541 gaatcaacag caccaattcc aaataccata tctgcaactg cattattctt ctcagcaata
     6601 acacggtctg ctaattgagc gccagggata tcaaccattt tgatattaaa accagcttct
     6661 tttgctttag cagttaacca atcaccacga ccatttgaga ctgagttcga atagattact
     6721 agttcttgac ttttatcagc tttttcttca gatgaagaag cagtcgtaga atttgaacct
     6781 ccagagcaag cagcaagtgt agtaagagca actcccgttg caagtacagt agaccaaact
     6841 ttcatttttt tcatgataag ttctcctttt ttattatttt atttaaattt ttcgtgatat
     6901 ggaacaaatt gtctcatatc ttcaaataca gtatagtcaa tacggtttac agtaatagtt
     6961 ggaatcttct ctaataaaat ttcagttaat tctgctctga ctttagtaaa ctcttcttcc
     7021 tcctcttcgg ttagaggaat ccgaagatac ccaattgaaa tatggaattg atatctatca
     7081 tgattaggga aacgaacacc tgctttttct gagacataag tacgaatttc ttctaatctc
     7141 tttgcagaag cttcatctgc aggttcaact agtatgtttt gttttcccat ttcagttaca
     7201 cgcatatgaa tttcttcatc caacaatgga aaaatttcaa gttgtttagc aaagtaatcg
     7261 tgtatttcct gtaaaggtgt atctagagga agattactgc tccaaaactc cgtttcacga
     7321 ttttcatggc acaacaattc aattacagtc atgtgaatag aattccttgg agttaaagta
     7381 aacttatcga taaatggtaa ttctctataa cgtgattgaa taatatcaac aacttccatc
     7441 aaatcttgtt tagtataaag atttgctaca actgtattcc cagggaaatg attaaattcc
     7501 ccattctctt tgaacttaat ttttgttaag tttttcatat aaagaccttc ttttttatat
     7561 ttgacatcgg tgtcatcatg tttacattat acaatgaaac cgtttccttt gtcaagcgct
     7621 ttcaaaaatt tttttaaaaa aatttatctt tcattaaata tgcatttcat taaaaggtta
     7681 agtaattttt ccaaaataca gcactcatat aaacagtatc atcaattata gccattggga
     7741 ttttttgatt gccaattcca tgtatctcta cacatgtctt caatcgattt tgttgtcctc
     7801 caatttaatt ccttatatgc tttgtctgca tttgcataac aagttgcaac gtctcctgaa
     7861 cgtcttggaa ctattttata aggaataggg atcttattaa cactttcaaa tgtatttaca
     7921 agttgtaata cactagtgcc ttctcccgag cctaggttat agatataaac atctgttttt
     7981 tcagatactt tttctaaagc ttttatatgt cctattgcta aatctactac atggatataa
     8041 tcacgcacac cagtaccatc aagcgtatca taatcatttc cgaacacact tagctctgac
     8101 agcttaccta ccgctacttg tgcaatataa ggcatcaagt tgttaggaat tcctgaggga
     8161 tcttccccaa ttaaaccaga ctcatgagca ccaattggat tgaaataacg aagcaacgca
     8221 atactccatt ctgaatctgc cacatgaaca tcttttaaaa tttgctcaag catcactttc
     8281 gtatacccat aaggatttgt cgcacttgtt tgcatcgtct caattagagg tgactgattg
     8341 ttaattccat atacagtcgc acttgaagaa aagacaatct ttttaacatt aaattctgac
     8401 atcacttcaa caagtgccaa tgtactcata atattatttt tgtagtacat cacaggcttt
     8461 tgcacggatt ctccgacagc tttataacct gcaaaatgaa ttgcagcatc aatcgattct
     8521 tgttcaaata cctttctcaa tgcttgttta tcacaaacat ctaattcgta aaacacggga
     8581 cgtattcctg taattgcttc aatacggtct agcaccaaga tgctagagtt cgaaaggttg
     8641 tcgacaatga taacttcctt tcctaaattt agtaattcta ctacggtatg gctaccaata
     8701 taaccagctc cgcctgttac caatattgcc atctgggttt cctcctaatt aattccaacc
     8761 gacttaacaa atctcataaa cgcttcatgc ccagacggtg tattcttata aactcctgca
     8821 tcttccagaa ctctcgcaaa cacttgtcct gcttcgtgtt gaactacgct attaacctct
     8881 tctttattaa tgcgaggata tttttctttc aattggtcgg cccattctaa atgataatcc
     8941 gcaattgcat tatcctctcc taaaagatat tttccaactt cttctaactc tggtttcaaa
     9001 cgaggtggta atatcgcaag tcccatcact tcgattaacc cgatattttc ctttttaata
     9061 tgttgtacat cttgatgagg atggaaaaca ccatctgggt attgttcagt agtatgatta
     9121 tctcttagaa caatatctaa ttcgtatctc ccgtccactt tacgagcaat aggagtcacc
     9181 gtatggtgtg ggacatcttc agtcatagca atgatgtcta cttctaaatc tgaatattct
     9241 ctccacttat ttagaatttt agtagctaaa tctaacaagc gatttttatt ttcactttgt
     9301 aacctaatta ctgacattgg ccattttaca ataccagcat taacatcctc aaagtcttta
     9361 aaacaaaatt cactctcaaa ttttgctttt tccattggga aaatatgttt ccctccctgg
     9421 tagtggttat gactaagaat ggagcctcct gagataggaa gatcagaatt tgaaccagca
     9481 aaatatcctg gcaaaatatc aacaatctcc aataattgtt caaatgtttt agaggtaata
     9541 gccattggta catgttgact attcaaaaat atcgcatgct cattaaagta tgagtaggga
     9601 gaatactgga atccccatac ttcgtcacca agtttcaacc gaataattct atgattcgaa
     9661 cgtgctggat aatttattcg cccctgatat ccttcatttt ccatacatag taaacatttg
     9721 ggataattag ttgcttttac taatttttca gcagcaattg tttttggatc tttttcgggt
     9781 tttgacaaat ttatcgtaat ctctagctct ccgtatttag ttgatgctcg aaactcaata
     9841 ttcttagcaa tagcagaagt tttaatataa tcactatctt tacttaactt ataaaactct
     9901 tcaactgctt cttgaggtga tatatcatat gaactccaaa aaatatcatt taatcgacta
     9961 ggtaaaggaa ctatgaaatt cattaactct gctcctaaac attccttttc ctcgattaaa
    10021 tctttaattt taccgttttt taaggcgatt tccactaagt aatcttttat ttgtttcagg
    10081 tcattttcat cggaaatgcg atcaattccc tcctcaccta ttaacgctag tactctattt
    10141 ttcacatata ttttgtcaat ttcattatac attccgtatt caattactct atcaacaaaa
    10201 ttatcaataa ttgttttcat atatttttct ttctaattta tgttcccata ttttctatac
    10261 attatccatt tataaattgc ttgcgtagta tgagcaattt tatcaaggtg atgaataata
    10321 tctaaagcac taattacttc agaaacgttc ccatcatctt caaatatgta attcattatt
    10381 ttcttttcca tatttatact aagctcttct atctcattct gtttttgtat aacaaccata
    10441 tctaaacatc cagattgttc ctctctataa caagatatag ccctattcat atgcagtccg
    10501 ataacttcat gaagtatttt tatttttgaa ataattttct tcaaaatttc attattttga
    10561 agaatctgta gattttttaa aatttcaaca attctatccc caatacgttc aatgtcagtt
    10621 gatattttta ttacactaat aattcttctt aagtcatatg aaacaggatg ttgtaaacaa
    10681 attaactcat atcctttttt atcaatattt agaactgact catttatgat taaatcttct
    10741 ttaatcaatt ctactcgttc ttcatttgat aaatattcaa ataacttctc atatttatca
    10801 agcacagata cccaaatggt ctctaaatta tttgataatt ctataatttc attttctaaa
    10861 tataacctta acatttaggt acctcttctt aacaaagttc aatagtaaca attaatattt
    10921 taaacaatat atcaaacatc aatgactaga atacttgcat catccttctt tccatagatt
    10981 ggatcaatag cagaagaatt aaatctcatc ttaattaact cttcaaaagt tttattttga
    11041 ttattttgat agaattcata aaagccatcg ctcattaaaa caatttgttc actagtaaca
    11101 tctatttgat taataatagc atggtctaaa aatctctcat ccaacgaacc tatccagtac
    11161 ccactcggtt gattagataa ttttctgatt ttttgtaaaa taattttttt atttaaaaca
    11221 ctatttgtac caattgaatc ttttatctca tttttccctt tttcaaataa gttatctact
    11281 ctatgatcag ttatttccat ttcgtttact aacatgacgc agtcacctag catcatatac
    11341 tccaactttt tttctgaaag tttagcaaat attggtaagc gataatatag tatattgaaa
    11401 ctagaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatt
    11461 gatttgtcct attattattt cattttacta tactctgtta atttatatga gtttaaaccg
    11521 atttcatctt taacctcgag taaagcagtt tcaaatattt gtttaagagt ttttgattct
    11581 ttacaattaa ccgacaaact ttctgataaa atatgtacaa cttctgagac tgaataacct
    11641 atctcctctt tagaattata taaatctgta gctccaccaa taatccaaaa atactgattt
    11701 tgtgaaccta caatatcctc attttctacg gaacttcctt gtatcgaaca aattttattt
    11761 atctttacca taatacttca acctttttag tgtcaaaagt aaaccaattc ctgtcactgt
    11821 taagaatagt tccataatct tattcgaacc agtctttggt aatttttgtt ttacatctac
    11881 tatttcttta gatttattaa tatgattttc agtttctctg ccatctccaa ctattttata
    11941 gtttacttct tctgtcttat tatcttgttt attgtcgatc ttgtcattca tttgtctatt
    12001 atctttactt gagttaaact ctccgttctt ctggttacta tcaattacat tatttgaatt
    12061 agattgtttt tcctctttgt ttttttcttt ttcgttttta tcacttaaat tatttgttac
    12121 aattttgtaa agcccattct ccgttacaat attgaaatta ccatcgctat cacgtataac
    12181 aggttctttc ccatttgcat tagatttgat gaatgatata tacttaccgg ataaattata
    12241 aaattggtta tttaaaacgg ttattttacc ctttgaatcc tcaataacaa ttccttcttt
    12301 acccccgaat atactattct ctgagactac taaattatca ctaactcgta ataattcaat
    12361 atttggttgt tctgtttcct tagaattatt attaattaca ttttttgtta cagtaatatc
    12421 tgatactttt cctaaatatt ctgcactatc ttttgcaact cgtatcgctt ttgttttagg
    12481 atcggcaata ttaaatatat tttcggcgat aaccacctgt ttatttaaat ctaataggtc
    12541 tttagtgttt ttatagctat aagcatttac taaagctgct ccgctttctc gataatgtac
    12601 actctctcct ttaactttct tatcaaagcg atttcctttg attaatacat cagtgaatcc
    12661 tgtaaaacgt acacctgcat acatcatgtt atcaaaatga ttattttgaa ttttaatatt
    12721 agaggggttc tgtgtcgaca atgtttgata gtgtgtgcca attgctgtta ctaattcccc
    12781 agatttatca cttttgccaa aataggaatt ttgaatagtc acattttcag attttttccc
    12841 atcatcattc aaggcataag gaaaaccttt tctagttaat ggttcaatct gaatgctctc
    12901 cttacttatg atttgcccat ccttcatcgt tttgggtaag gcttgcccaa gaaaacgaga
    12961 attatcaact aatacatttt tcgaacctgc aatttgaata gcatgccctt gataactatc
    13021 cttgaatgtt acatttttta tagttacgtt atttgaattc ccaatagcaa atgcacctga
    13081 agaatttata agtggtagat tttttgcttt agttccttct tcattcaaag caccgttcat
    13141 atcaatcgta ccaccagaat aactaatatc ttctgttggg ccccattcta cttgcgcacc
    13201 atcatccgta aataaacctg tcataaaaac aatggaaggg tgattcttaa tatttatacc
    13261 atttagaatt gtagctttct cattcaattc taagtgtgta tgacttttta aaaaaacaat
    13321 ttcttttact aaataagttc cttcaggaaa atatacattt cctccaccta gcccttgagc
    13381 tgcagcatct attgcatctt gaattgcttg tcgatcatca tgaatcccat cacctaccgc
    13441 accataatct tttacactta aagcagtatc ttcaaattct tttttgataa ttctgtcact
    13501 tataacatct tccttataac tcttattttc attagtagat gttactgtag attcaactgt
    13561 tctgttgtct aattgtgaat tggaacgaat agtaccatta gagctgatac tattaacact
    13621 taacgaatta tctaaaccat tagtggttga attagaggca atcatttcag cagaacgatt
    13681 ctgattaaca gttggtgaat tatctaaatc attagtaatt gcattagaag actcttttgt
    13741 agccatcggt gaagaactag taacaacttc atccgataaa ataccttggc ttcccaaaat
    13801 tgaaattgcc aatagtaccg agcaagtccc cacctttaat ttacgaatac tgaatacctc
    13861 attaggaaca aaatatttca tcagaacctc caattttttt actattttta acaattgatt
    13921 attacttttc ttaaaataga tagaatgaca ccgctgtcat tatctttatc atatattttc
    13981 caaattgaat tgtcaaggaa taacacaaga aaatattaaa atttatactc ttcggaaatc
    14041 tcttcaaact aggtcagtgc cgccttgccc taagtctagg ttactgactt ggtcagttct
    14101 atagtagact gaaactaaaa tagtacacct ctacttctaa aacattgtta gaaatcgatt
    14161 tgactgtccc gatcaatttg tcctattctc atttcatttt actatatcta caacctcaaa
    14221 gcagggtttt gagctatcta tggccaactt cctagtttgc tctttagcct tccattcaaa
    14281 agtgatgcta tctttcttag cttgaatata aaatatccca tttactatat ttaaatttgt
    14341 tgatatgttg taaattataa atgaaagaac aaataccttg tcatacaagc attcgccagg
    14401 gatatttacc tgaatatcgc tcatatggcg gattgaaact aaaacactac aatatatttc
    14461 ttaaatagac cttctataaa agctagattt aacctcgata aaaatagatg cagtgcttct
    14521 aaaatactgg aatagggcat ataggtaatc gtagacaagt gttttataga ttgtctcgca
    14581 ggagatctaa taatgtatag caagcatcac taaacatata gaatatttgt attattataa
    14641 tatcaacatg ctataaattc tatcatgcaa aagaatgact agaacatgaa agacaggcac
    14701 aaaattttga acaatattct gaaaacaaaa aaagacgcga gtttccacaa tctcactcat
    14761 ggaaactcac gtctttcaaa aaatagggtg aattgaaaac tagaacaaga cactagattt
    14821 tctaaatgat gtctagtaag gattctaaat gctacactaa attgctcaca tcttatttca
    14881 aaccacggga ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagaaag
    14941 gggcctcaac tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt
    15001 agacttttta ggggcaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag
    15061 ttagcacttt ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc
    15121 ttaatttagt atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat
    15181 agttcgtcat atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt
    15241 gaacattact tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta
    15301 ggcttgctcc ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct
    15361 tgtgtgccaa aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatgt
    15421 ccttacggcg ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca
    15481 tgatatcggt catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca
    15541 gttgcatctt ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct
    15601 tgtacatctc ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg
    15661 ccgttgtaaa gttcttaact gcatggaatg agaaggaagt aaagtcagcg atagaaccag
    15721 aaggttgtcc tttataaata gatcccaaag cgtgggcact atcagagaca atgacaatac
    15781 ggttaaaggc cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga
    15841 cttggaacaa acggtcataa tcgcaaacaa tccctgcgag ctctactgga ataatcacct
    15901 tagttttctc agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt
    15961 ggatatccac catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat
    16021 aggtcatggc tggaacgatg acttcatcac caggtcccac ttccaaaacg cgtaaaatca
    16081 actccagagc ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag
    16141 acaagcggcg ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg
    16201 tatccactac ttcagtaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt
    16261 aatttggcat tgatttgctc cttttatctg tactcatttt cttatgacta ctttatttta
    16321 gtacctcaaa cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga
    16381 gataggcgag gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa
    16441 ctgacagacc tttctccgtc atttgactga taattgtgtc ctcatccttg tagttgatgc
    16501 tggctggaga ggtaatccct gcttgcaaga gcaaggttgc catcatttca gggctatact
    16561 gctctgtata acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga
    16621 cattgaccaa ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg
    16681 taatgcggct atcgttagca gaagtcacca gacttccttt tttatccgca tccgtcacca
    16741 tggtacgaaa cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt
    16801 aaatcactgg ccctttgcta tccaacttga tccaaatgct caagatgaga aagatgggag
    16861 aggtcagaac cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg
    16921 aacccttcct tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca
    16981 caggcaagtc ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca
    17041 acatttttca ttataccaca aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga
    17101 tttccttcta gtactcaatg aaaatcaaag tacagctttg agattgcaga taaagctgac
    17161 atggtttaaa gagattttca aagagtatcg gccatggccc ctgcctgcaa actatacatc
    17221 ttgtgatagg ttcctcccag agccaagagt tcctcatggg ttccactctc gataatgcgt
    17281 cccttatcca agacatagat gcagttggca tcttgaatag tagaaaggcg gtgagcgata
    17341 gcaatagttg ttcggccctg tctcatcttc gccagagaag cttgaaccaa gctttctgtt
    17401 tcagagtcaa tattggctgt cgcttcatcc aaaatcagga ttttaggctg gctggcgact
    17461 gttctagcaa aggcaagaag ctggcgttgc ccagtagaga agctcgaacc acgctcggaa
    17521 acaggggagt cgtacccctg aggaagttct tgaataaagg aatctgcatc cacaaaggct
    17581 gccgcagcct gaacctgctc atcactggtt tcttggtaca tggcgatatt ggacttaata
    17641 gttccatgat agaggaaggg ttcctgcaag accaaaccga tgttttttct cagctcttct
    17701 tgactgaaat ccctgatatc cacatcatcc aagagaactc tccctgactg gaattcataa
    17761 aagcgcatga ggacattgat aatagacgat ttccctgaac ctgtatgacc tacaaaggca
    17821 atggtttcac ccttattaac agagaaagaa atgtcatcca gaatcggatg tttaccgtca
    17881 tatgagaaac acacatgttc aaaacggata ttgccttctt ggactttggc ttgcccattt
    17941 tcttgaagag gttcataggt cctctcgtct atcagggcaa agacacgacc tgcagaaacc
    18001 atagccgttt gcagagttga aaagttttgc gtcacctcaa tcaaggggtc aaaaaggcgg
    18061 ttgatgtact ggataaaggc atacatggtc ccgaccgtta tcccgataga aaaaccacgg
    18121 tagccaaagt aggccatcaa gactgcatag cctagaagtt tcagcaaact catggcaggt
    18181 ctcaaaaaga gggcatccaa ggctacagaa cggttggcgt agaccaagtg ttcttggttg
    18241 atttcatcaa attctgcctg caggcgcttc tcttgattaa aggcctgaat aatcctgatt
    18301 ccctcgatat tctctgccag cttactattg atatctgaca agagacttct ggttttctcg
    18361 atgattttca ctgacttttt tcgatagaga ttgaccaaaa ggaaaatcaa aggaagaaag
    18421 agcaagacta aagccgtcaa acgaaaatcc agcaccaaca tggtataaag ggttgtcaga
    18481 aagataaaaa ctgctgagat aaagctggat aaaatcccag aaaacatatc actaatcgtc
    18541 tcggtatcgt tggtcaaacg agaaacgata gaacctgctg gcgtcttgtc aaagtaagac
    18601 atgcccagtt tctccatatt ggcaaaggca tcccgacgaa tatccctaac aatactgtaa
    18661 gacacgcgcg caaagagaag attgccgaca tactgaacta cagtttgtag gatgtagaga
    18721 ccatagtaga ccagcaaaac ggtaacggct agttggttaa gattgctgag atactggtcg
    18781 ataaagtggg aagccacgag gggtatgaca cttttaatga ccgtcgtcgc taggagaaaa
    18841 ctgagtgcca aaaaggtcag gagtccataa ggcttgagat aagacatcaa gcgcttcaat
    18901 acagtccatt gttcttgttt attctgcatc ttcttctcct ttcatttcca actgctgaga
    18961 ctggtaggtt tgggcatacc agccatccaa agctagcaag tcttcgtgcg tgcctcgttc
    19021 gataatttga ccattttgta gaactaaaat aaaatctgca tggacaacag cactgaggcg
    19081 atgggcagtg ataatggttg tcttgtcctt tcgcatctcc ttgaggttgt cgataatcgc
    19141 atactctgtc ttggcatcta cggcggataa ggaatcatcc aaaatcaaga tatcagggtc
    19201 taaaatcata gcccgactca tagccaaccg ttgcttttga ccaccagaaa gactgactcc
    19261 tttttcacca atcagcgtat caaatccttg aggcatgtct acaatatctt ggtaaacccg
    19321 ggctagctta gtagcttcct cgaccgctga aaggggcaag ttaggattgc caaagcggat
    19381 attgtctagg attgaagtcg caaaaagaaa ctggtcctga ggaacatagc ccatgagact
    19441 gcgaaggtct gtcagacgat agtcccgaat atcgtgaccg tttagataaa tggcaccctt
    19501 atccacatcg tattcacgca agaggagctt gattaaggac gttttcccag agcctgtctg
    19561 cccaaccaag cccagtgttt gcccttttgc caaactaaag tgaatatccg tcagtgtttc
    19621 ctcattttca aaagcaaagc tgtcaatggc atactccaaa cgcccatttt caataccatc
    19681 cagaggaaac tcagggtctt gtacaggaga ttcctgagac aaaagatttt caatccgctg
    19741 gtaagaaacc ttccctcgct gagtagtatt aaagaggaaa ccgatggcca gaagaggcca
    19801 gaccagcata tccaaatagc tgataaaggt gactagattc ccaactgtaa tctgcccttc
    19861 ctgaaccatc aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa caaacaagag
    19921 aaccataggg tcaaagagac tatcatattt catggtttgc aggttctttt ggaaggttaa
    19981 ttcattgact gcctgaaaag acttcaactc gtctgcctga taaccgaaag acttggtcac
    20041 tttgatacct gatacggact cctgtacctt gttattgagt tcagaaaaag cagcttggga
    20101 ttcgccaaag gccttatgag tctttctccc taggcgacta gtcgtatagg ccatgaaagg
    20161 tagggggaga atggcaacaa gagtcatctg ccatgagatg ctaaagagca tggtcaacaa
    20221 agtcaccaga gccgtgatag aggcatccac cgcagacatg acaccgccac ctgctaaacg
    20281 agtcaaggca ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc gataggtttg
    20341 ataaaaggct gacgacattt ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc
    20401 caagcaataa gaggtcccaa ggatatacat acgccacaca tagcgcaaat agtacatacc
    20461 aaaggctgca agtagcaagt aaaataggct aagaaggagg tcctgctggg ttaattgccc
    20521 cgatgtgatg gcatcaatga cccgccccat aaccatagga ggaatgagat tgaggacgga
    20581 aaccaagatc agggccacaa ttccgactag ataacggcgt ttttctaact tgaaaaacca
    20641 ccaaagtttt tgaataatgg acataaaatc cctttctgat tgcaaatgga aacctgaggc
    20701 caatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaagcac
    20761 tgctttgagg ttgtagatag aactgacgaa gtcagtaacc tacatacggc aaggcgacgt
    20821 tgacgcggtt tgaagagatt ttcgaagagt acaagacccc aggtttttct tattcataag
    20881 ttacgactgt aacagcaccc ttgtcatatt cagcaataaa gatattggct acattgtcat
    20941 gcccttgttt actgaggtta tcaagcaacc actcttcgct acgaccaatc gattccaaga
    21001 catctacttg aatcacaccg tcagtcacaa ctggatactt aggattttca tctcccattt
    21061 gcacaacgat gagttgccca ttttgctctt gcacagctcg tttgacttgc ttcatctgga
    21121 aaatcccttg gctacgaagt ttgagggata catccgatgc agacaaacca actgaacgac
    21181 aggcttctgg gtcaatattc ccatttttga taaggagagt tggcttacca tcaatcaaac
    21241 gtttcacaaa gtgaacattg ttgttgagct atttgagagt caagaccagt ttgattaaaa
    21301 tttcgatata attaagtgtc atttgttcac atccaccagt tcaattgcgt ctgtcttata
    21361 caattcgact ttctctaaca ggtacttgtc tggctcactt ccatttaggg cacgataata
    21421 gcgatctccc accttgataa gtgcgccatc tgtgtttgtg gaagtattaa catagacttc
    21481 tgacttgtct actcacaaat ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa
    21541 tttattgtta gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa
    21601 ataaggccag atctgaaatc atgaccaact cacgaaattc gcttcccttt ttattatggt
    21661 atgccttgaa agcaaaaaaa actgtcacag ctaatagaac aatcccaaag ccaaccatga
    21721 taccattttg ctgaccgatt tggctgagta catagtcata agagtagaat ttcatgtatt
    21781 ttcactccct ttcataaaat cattcttaat tataaacgaa agagagtaat ttttcaaacc
    21841 atacgttggg gaaatagatc tttttttggt ataataaaat ctataatctg aatgaaaaag
    21901 gtaactttat gaaacttatc tcatggaata ttgattccct aaacgctgcc ctaactagtg
    21961 actcagctcg tgccaaattg tcccaagaag tcctacaaac cttggtcgct gaaaatgctg
    22021 atatcattgc tatccaagaa accaagcttt ctgccaaagg acctacaaag aaacacgtgg
    22081 aaattttaga agaactcttc ccaggctacg aaaacacgtg gcgctcttcc caagagcctg
    22141 cccgtaaagg ctatgctgga accatgttcc tttataagaa agaacttaca cctactatca
    22201 gcttcccaga aatcggtgcc ccttctacca tggacttgga aggtcgtatc atcactctag
    22261 aatttgatgc atttttcgta acccaagttt acactccaaa cgctggtgac ggtctcaaac
    22321 gcttggaaga acgccaagtc tgggatgcca aatatgctga gtatttggct gaactagaca
    22381 aagaaaaacc agtccttgcg accggtgact acaacgtagc ccacaatgaa atcgaccttg
    22441 caaatcctgc tagcaaccgc cgttcacctg gatttactga cgaggaacgt gctggattta
    22501 ccaacctttt ggcaactgga tttacagaca ccttccgcca tgttcatggc gatgttcctg
    22561 aacgctacac ttggtgggca caacgcagca aaacttctaa aatcaacaat acaggctgga
    22621 gaatcgacta ctggctcaca agtaaccgta tcgctgacaa ggtcactaaa tctgatatga
    22681 ttgactcagg tgctcgccaa gaccatacac cgattgtctt gaaaattgac ttgtaaggat
    22741 tttctatatg gactacaatg cggtcattcc cgagttgctt gtatcgaata tcgaacagtc
    22801 ccggtccttc tactgtggtt tactgggttt taggattgaa taccaacgtc cagaagaaaa
    22861 ctttctcttt ctcttgaaga gtgtcaacta atgttagagg agggaacaaa ggatcaacta
    22921 gctgaactga cctatccctt tggtcgtgga gtcaatctct cctttggcat aaaggatgtt
    22981 tcaaaactct attaaagagt gatgaaggca aactatccta tttatcgtcc cttgaccaaa
    23041 agaaagtttc gtgtcagtga tccctatatc tatcctcata aatttgcagt tttggatcca
    23101 gatggctatt ttttaagatt tagcgagtag aataataaaa attgggaaaa gtctttaaag
    23161 ttagaaaaag gcatattgtc aggtgtctaa aagcttgata gtatgccttt tatgatggaa
    23221 aaatcttcta atactcttcg aaaatctctt caagccacgt caacgtcgcc ttaccgtagg
    23281 tatatgatac tggctctgtc aattctatcc acaacctcaa aacagtgttt tgaggttgtg
    23341 gatagcttcc tagtttgctc tttgattttc atttagtata actgttagct tacaatggag
    23401 tttttgttca ccctctcttc tttttgttcg ttttttaaac caaaacacta acaaaatagt
    23461 tgatttctaa agttatttag tgtaaaataa aaacattggc tcaagcctat ttaaaattga
    23521 atatcgtttt acttgtttat gtcgtgaatt ggcacgacgt ttctacaagg tgccggaaca
    23581 cctaacaata agtaagtcag cagtgagata tggccgtttt tgccatgtct atttagggga
    23641 cttacttaga gattaagtag gtcttttttt aatatttccc ttaattagtg cggacgggag
    23701 gtgaaattat cctgtgaata atttcaggaa tccagggaat ttcatcccta atttttgagc
    23761 ccctagctct ttcatttcta ggcttaggct aaaatagttc cccgaactat tttactctca
    23821 aaaatcccgt catagacaat aacaagatta ttgtctatga taccactaca acgggaaata
    23881 tttgataaga ctcgcttcgc tcgtaatagt tttatcaaat atatagtatt agcaaaaggg
    23941 atcactatag aaatatttaa acaacaagta aattaacttt tagactttag gaggtcttat
    24001 gaaattatta gaagagcgca tcctcaagga tgggcatatc ttgggtgata acatcctcaa
    24061 ggtagattcc tttttaaccc accaagttga ctttagcttg atgcgagaga ttggtaaggt
    24121 ttttgcggaa aaatttgcta ctactggcat taccaaggtc gtaaccattg aagcgtcggg
    24181 tattgcccca gccgttttta cagctgaagc cttaaacgtt cccatgattt tcgccaaaaa
    24241 agctaagaac atcaccatga acgaaggcat cttaactgct caagtctact cctttaccaa
    24301 gcaggtgacc agcaccgttt ctatcgctga aaaattcctc tcaccagagg acaaggtttt
    24361 gattatcgac gatttccttg ctaatggcca agctgctaaa ggcttgattc aaatcatcga
    24421 acaggccggt gccacagtcc aagctatcgg tatcgtgatt gagaaatcct tccaagatgg
    24481 tcgtgatttg cttgaaaaag caggctaccc tgtcctatca cttgctcgtt tggattgttt
    24541 tgaaaatggt caagtcgtat ttaaggaggc agatctctaa tgcaaactca agaaaaacac
    24601 tcgcaagcag ccgttcttgg cttgcagcac ttactagcca tgtactcagg atctatcctg
    24661 gttcccatca tgattgcgac agcccttggc tattcagctg agcagttgac ctacctgatt
    24721 tctacagata tcttcatgtg tggggtggca accttcctcc aactccaact caacaaatac
    24781 tttgggattg gactcccagt cgttcttgga gttgcattcc agtcggtcgc tcccttgatt
    24841 atgattgggc aaagccatgg tagtggcgct atgtttggtg cccttatcgc atctgggatt
    24901 tacgtggttc ttgtttcagg catcttctca aaagtagcca atctcttccc atctatcgta
    24961 acaggatctg ttattaccac gattggttta accttgatcc ctgtcgctat tggaaatatg
    25021 ggaaataacg ttccagagcc aactggtcaa agtctcttgc ttgcagctat tactgttctg
    25081 attatcctct tgatcaacat ctttaccaaa ggatttatca agtctatctc tattttgatt
    25141 ggtctggttg ttggaactgc cattgctgct actatgggct tggtggactt ctctcctgtt
    25201 gcggtagctc cacttgtcca tgtcccaact ccactctact ttgggatgcc aacctttgaa
    25261 atctcatcta ttgtcatgat gtgtatcatc gcaacggtgt ctatggttga gtcaactggt
    25321 gtttatctgg ccttgtctga tatcacaaag gatccaatcg acagcacgcg ccttcgcaac
    25381 ggataccgcg cagaaggttt ggccgtactt ctcggaggaa tctttaacac cttcccttac
    25441 accggatttt cacaaaacgt tggtttggtt aaattgtcag gcatcaaaaa acgcctgcca
    25501 atctactacg cagctggttt cctggttctc cttggactgc ttcctaagtt tggcgccctt
    25561 gcccaaatca ttccaagctc cgtcctcggt ggtgccatgc tggtaatgtt tggttttgta
    25621 tcaattcaag ggatgcaaat cctcgcccgt gttgactttg ctaacaatga acacaacttc
    25681 cttatcgcag ctgtttcaat cgctgcaggt gtcggtctca acaacagtaa tctctttgtc
    25741 agcatgccga cagccttcca aatgttcttc tcaaacggaa tcgtcgtagc cagcctactc
    25801 gctattgtcc tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga ggtgcgagcc
    25861 tcttttttgt tgacaaaata aaaacatcta cctattcata tatgctagtg tcatagtcct
    25921 gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt acaagtcgtt
    25981 cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt tctgtaattt
    26041 ctactgtata ctttttcagt tttagattgc tttccaataa ttcacctatt aacaataatc
    26101 tatgatagga gctatgtagc cactctggca atttatctcc tacttcccac ccatattttt
    26161 ccttcatcaa agctagttgt ttttcgtcac caaatttcat attttcagat aaccatctca
    26221 cacaatcggg atctgataag gtacagacga ttaaacttag gttatcgtaa cggctttcag
    26281 tttcataatc aggccagatg acttttaaga aatcatacac ctcttctgat gcaacaagtt
    26341 gtttcgtttt cataatttcc gatactttcc tctaccttta aattctatta tttctttgtc
    26401 tcttaatatt tgaagctgtt gcctaatctt ttctttgata tgattgttct taacaaagat
    26461 attttttagg tcactttcaa aacgatacat atcttcaagg gtaaattctg aaccctctat
    26521 cttatctata caatttagaa tttctattgt ccaacctctt gatgacagag agctcttcct
    26581 taaaaataaa ccttgcttaa attcttttgt aactttttct ggatctctaa cttgtccatc
    26641 ttgcacaaga aatatccttc ctttagaagg tacttgtgat aaatcaatgt tacaaccaat
    26701 ccaacctgct cgtctagcag ttggtgcaag tggttttctt tgaataatcg atttcggtgt
    26761 aacaaattgc ttcggaagga caagaaagtt atttacctca aaattttttg tgtaagttaa
    26821 aaaaaagaaa ttaggattat tatctgcctg cacacgcttc atcatcgttg cataagcacc
    26881 atcattgatt gttgatgaaa aatttccttt tttgctcttt agttcaaact cctcactaca
    26941 atgattacag taaaaatctg ctacaggccg attattttca aaatgattta agggattgtt
    27001 cccacaatta gggcaataag actgacgata aacccaatcc tccgttaaga tacgtgcctt
    27061 ttggctatta cttttatagg tttctactaa ttctaaatta aaatgtaatt ccatttaaaa
    27121 attcctcact aatatgagaa ctcactcgat ataagcaatt aaaatctact ataactagta
    27181 taccacatat atgtacctat tacatttgta tttcatcact ctcttttata tttccttcta
    27241 acatatctca taaattaaaa atgagtgaga taaatttatc ccactcacct ttctttatat
    27301 ccttacccgt ctttcctcag acctttggcc tgtgacaaac atggtaaagg ttgctttgca
    27361 gacatttctg ccctcttgat tggtgatatc cacatccatt acacaagttg tacgaccttg
    27421 atggacacat tctcctttaa tggtcaatac atcgtcgagt tttcctgcct taaggtagtt
    27481 gatagaggat tggagtgtca ccccatcaag tcccagcgag ataaccacca aaccactgat
    27541 ttggtcacat agcgtgaata gatagccacc atgggcattg ccatagtagt tgagcgacga
    27601 gttcactact ttggtcgtca ccacaacgtg accatctctc attttttcaa tttcgtaatt
    27661 ttcaaaggca gatatagcgt caaaatgaaa gtctttcatc gtttcctcct gatatttcaa
    27721 acgacactat gatactactt tttataggac tgtgcaagga gaaagctcct agtctagtaa
    27781 aattccgacc tgttccacaa agcgcataaa ggctgtctgc ccttgttctg tctgcttgta
    27841 gactcctgca tcctcaagta cacgcgcaaa gatagcaccc acagagtcct tgacgattgc
    27901 aagggctttt tctttatccg ttaggtctgg atgttgggat ttgagttggt ctgcccactc
    27961 ctgatgataa tcggcaactg taacagcttc tcctacaaga tagctagcga cttgctccac
    28021 ttcttctttc agacgtggtg gcaagattgc caagcccatg acctcaatca agccgatatt
    28081 ttccttcttg atatgttgga catccttgtg gggatgatag ataccatcag gatactctgc
    28141 tgaagtctga ttgtctcgca agaccaagtc caactcaaac tgtccatcgc gtttgcgggc
    28201 aatgggtgtg atagtgtgat gcggtgtcct gtctgtctct gccaaaatct gcactgcagg
    28261 atctgaatac tggcgccatt cctgcaaaat cttatcagcc aaattgatca aatcctcttt
    28321 ggaatccgaa gtcaaacgta ggacagacat gggccacttg acaattccag ccttgacctg
    28381 ctcaaaacca gcaaatcgga aggccttttg caagggagcc aattccatag gaaatacgtg
    28441 acggcctccc tgataatgat catgagttag aatagagccc cccacaatcg gcaggtcggc
    28501 attagatcca gcaaaatatc ctggaaactg gtctacgata gccaacagac gttcaaaact
    28561 ctgacgacta atggccatgg gacgatgctg gccatctaaa aagatacaat gctcattaaa
    28621 gtaagcatag ggcgaatact ggaaacccca ttcctgacca accatttcaa aacggataat
    28681 acggtgattg ctacgagctg ggtggttaac tcgaccatgg tagccctcat tctctagaca
    28741 aagctgacac tgaggataat tactagcttg caccaacttg gctgccacaa tctctttggg
    28801 atctttttca ggcttagaga gattgatggt aatttcaagt tctccgtagt cagatggaac
    28861 acgataagcg atatttctag caatggcctt gagtttgatg tagtcatttt tctgactgag
    28921 ttggtaaaaa tcctctatcg cttgttctgg agagtgggcg taggttgccc aaaaatcacg
    28981 attgacctga cttggacaag gagtcaccaa atccatcagt tcagcaccaa ggatttcacg
    29041 cgcagtctga ctatcctcaa tcgtctctaa tcgaacggct tcttcaacca gctggtcctt
    29101 gaggtcaatc aatttatcca gattggtctc aacttccaaa acaccttctc ccactcgtgc
    29161 caaaacacga ttggtcagat agattcgatc catttcctca aatgagcttt cagaaatgac
    29221 atgtgttaca aatttattta ctaaggtcac tatagagcct ccttttgact agtcaaggac
    29281 gcgagtgcca cctgcaactt cagcgatata gaagcttgga gcgtatccaa ctacttcctc
    29341 gtagtgtttg cctacagctt ccttaaaggc ctcaacagta tctttttgaa ccaaggcaat
    29401 ggcacagcca ccaaaaccag cccctgtcat acgagcaccg agaactcctt cttgtgccca
    29461 agctgtgtga acaagggtat ccaattccaa accagttact tcataatcat gctccagaga
    29521 aacgtgtgac gcattcatca agcgtccaaa tgtttccaaa tctcctgctt ggagtgctac
    29581 ttgagctttg agggtacgtt ggttttcaag cacagcatgg cgagcacgtt tcaaacgatt
    29641 ttcatcttta atcagatagc tatattggtc aacggcccac tcgtccaatt cacccagagt
    29701 ctgaatatcc aaggaaactt gcaattcttc cactgctttt tcacactcag cacgacgttc
    29761 attgtattta gagtccgcca attcacggcg tttgttggtg ttcatgataa caacgacatt
    29821 gtccttcaaa tcaagtggca ccaagtcgta ttctaaagta ttagtatcta ggtaaatagc
    29881 acgttggtct gcccccatac caatagcaaa ctggtccata atgccagagt ttactccgat
    29941 aaagttgttt tctgtttgtt tgccgatttt aaccaaatcg agacgctcta attttaaatc
    30001 aaagagatgc tcagccacga ctcctgtcaa gagttccaag gatgcagaag aagacaagcc
    30061 agcaccattt ggaatatttc cataaacata aaaatcaaaa cctttgtcaa tcacgtgccc
    30121 agcttcttgc aagaaatgaa ggacaccttt tggataattg gtccagttgt gctctttttc
    30181 aaacttgagg tcagcgagag gcacttcgat aatgcccttg tcctcaaagt tagctgagta
    30241 gaaacgcaag acttggtcgt cacgcttacg agctgcaccg taagttccca aggaaatagc
    30301 agcaggaaaa acgtgcccac cgttgtagtc tgtgtgttca ccaatcaaat taatgcggcc
    30361 tggtgaaaag aaggtttgat ctgcttcttg accaaaaaca gcaagaaagt ctttgcgaag
    30421 ggcttcagta gtaagatgtt gtgccatatg aattctcctt tgactgtccg ttaagccatc
    30481 ttaacgtgta atcgttttct ttctattata tccaaggaat tttccaaggt caatcctttt
    30541 tactaaaatt ttagtaaaat atcagtaaaa agaagaaaaa catgatatac taacctaatc
    30601 aaggaggatt tatggctacc ttaaaagaca ttgcacagct agcctctgtc tctatcgcga
    30661 ccgtatcccg cgtcctcaac cgcgaccaga gcctatctgt tacagaagaa accagacacc
    30721 gtattttaac cgttgctgaa gagctgggct acaccaagca cctcaagaca ggcgagtccc
    30781 acaaacccaa gcaaaagatt gccattatcc aatgggtcag cgaacaaggg gagctggacg
    30841 acctctacta ctaccagatt cgcctaggaa tagaaaaaag agcccaagag ttggactatg
    30901 atatcttgcg ctattttaat gaccaccctt ttaccctaag cgaggaagtg attgggattc
    30961 tctgcatcgg aaagtttagt cgagctcaga tttctgcctt tgaagaatac caaaagcctc
    31021 ttgtatttct agacagcgat acactttccc tgggacatac ctgtattatc acggattttt
    31081 acactgctat gaaacaggtt gtcgattatt tcctcagtca aggaatggac cgtatcggga
    31141 ttctaacagg ccttgaagaa acaacagacc aagaagaaat cattcaggac aagcgtctag
    31201 aaaacttcaa aaactatagt caagcgaggg gaatctatca tgatgaactg gtctttcaag
    31261 gaagatttac tgcccagtct ggctatgact taatgaagga ggccattcag agcttgggag
    31321 accaacttcc gccagcattt ttcgcagcca gcgatagttt agctatcggt gccctccaag
    31381 aagctggaat cagcctgcca gatcgcgtca gcctcatttc ctttaacgac actagtctga
    31441 ccaaacaggt ctatcctccc ctctctagta ttacagttta tactgaagaa atgggccgag
    31501 caggtatgga tattcttaac aaggaagtcc tccacggtcg gaaaatccct agcctgacca
    31561 tgctgggaac cagactgaca ttaagagaaa gtaccctaaa tcaagaatag gataacataa
    31621 aaaacgaata gagttctaaa actcctattc gttttttatt cgattacaat catagactta
    31681 attgtcttac gttcatccat atctttatag gcttggtcga tatcttccag tttataactt
    31741 gaagtaaaga cgcgacctgg attgatatca ccatcaagga cggcttttag taaaaattgc
    31801 ttatcgtatg ttgtagcaga agctgcccca cctgctacag agatattttg cataaatgtc
    31861 gaaccaagag cacgattatt atagtgtggg actcctacaa agcccatacg ccctccatta
    31921 tgaagaacac ctagcgcctg ttctatagca gcctccgtac caacacattc aagtgctgca
    31981 tctgctcctc caccgaggat ttcacgcacc ttggtaattc cttcttgacc acgttctgca
    32041 acaacagctg tcgcacctga ctccatagcc atcttttgac ggtcttcatg acggctcata
    32101 aggataattt gtgatgctcc acgcatctta gccgcgatga cagcacattg accaacagcc
    32161 ccatcaccga taacaacaac cttgtcccct ttttgaacat ttgcaacacg cgccgcatga
    32221 tagcctgtcg gcatgacatc tgcaagagtc aaaagggact tgagcatccc ttctgtatag
    32281 tcagaaggtt gaccagggat tttaaccagc gcccagtttg catagtggaa gcgaatatat
    32341 tctgcctgaa aatcaccccc caaattattg ccaatatgat tgtcgcaaga accgtcaaat
    32401 ccagcaagac aggcatcaca ctcaccacat ccatgtgtaa aagggacaat cacaaaatca
    32461 cctggtttca ccgtcgtaat ggcttcccca gcttcttcaa caatcccaat cgcttcgtgt
    32521 ccactatttt tgtgtccagc tttcgtttct ggattacggt acctccataa atctgaacca
    32581 caaacgcacg cacgaaccac acgaataatc acatcatccg cttctattat ttgcggacgt
    32641 tcaatgctag caagtccaac ctgacctgcc tttgtatata ctgctgattt catttaaaat
    32701 tttccttcct tataaagttt aattttgaga tttaaacgat ttaaagctgt ctgtagctga
    32761 gacaagcgct cctctaattt ttgcttttcc tcttctaaaa taccaagcct ctcctctctc
    32821 gtttcatctc ccttttggta gagcgacata tagtcaacta aactatctac agagacaccc
    32881 gccgaacgaa aacacttaat aaattccagc gcttcgatat cctgatcttg aaaatcacga
    32941 atcccagtag cagtacgagt aatcggtggc acaagaccaa cccgttcata ataccgaatc
    33001 gtatccgctg aaattcccaa caaatcactg gcagatttaa tattcacacg ctaacctcct
    33061 ctcatttcat tcactgaatt cacgctattc taactcactt atcttattat ataactttga
    33121 gttgactcca agtcaagcat tttgtatcta atcgaaaaat ttttatcatt aacaatcata
    33181 tatctggagt ttatacaatg ctcttctctt accaattttt gaaatattta aataataaag
    33241 aaattactct attccaaata agatagtacc caagtatttt tcactttttt ctaatgttga
    33301 tgctcataac tccgttccaa gtcacacacc tttcgcttat gggtttggtg agtttctagg
    33361 tcagcatcaa tttcaatggt aatattttga aaaccacaat cttttaggaa aattcgaata
    33421 gactctttac aagtttccat atgttccatt tcttttagac aaacatggac aatggcattt
    33481 ttttccaaag catccatagt ccagagatta agctgattaa ggctggccac attgtccaat
    33541 cgctccaggc cactctttac ttgcttgata tcaagacctt ctggcacagc atccaagaaa
    33601 atcttgagtg tagaccaaaa acgtggaagg gcttttgaaa gaataaagaa agaaatgaca
    33661 agggacaaaa gaggatctag gatataccag tccgtaaatc gaagaacaat cgccatcagg
    33721 ataacagcta cccaccctag cgtatcttcc agaaaatgca gactcagaat agactcattc
    33781 tttgtctttc ccttaccaac caccagactc gctaacagat tgatagtaat cgcaataatt
    33841 cctaaccaga gaatcccctc atcattgact ggttgcggat gcaaaatctt cgtgacattt
    33901 tccaaaatga ctagaacaga gcccgttacg agaatcacag ctgttaccaa ggctcctagc
    33961 aggctaaacc gcttatagcc caaggtgtac tgattgtctt cttcacgatt ggagattgtt
    34021 tctagaaaag ctgatattcc aattgcaatc gcatctccca agtcatgcac agagtcagca
    34081 agaacagcgc tagaaccaaa tactccacct gcaataaact caacaatggc ataagtcaaa
    34141 tttaagaaaa aagccaccca aacagcatat tttgccttca tatttctcat tcctttgtta
    34201 taatagattt atgaacacct tgttcattat cattatccac taaaactcaa agaaaggata
    34261 gaagcaaaac gtcagcttta ttcagttctg aacaatttgc cttaagtgtc catatgacta
    34321 acattgaccg ccgtatcagc aaaaccaaaa aagccatcta tcaagctttt atacaacttt
    34381 tgaatgctaa gggctacgag gccactactg ttcaggatat cattgatctc gcagatgtgg
    34441 gacgatccac cttttactgt cactatgaga gtaaggagct tcttctggac cagctctgcc
    34501 gctacctctt ccatcatctt tttgaaagag agcaagccat ttcaaccgag gactacctcg
    34561 cccacctctt tctccatttt cataaaaacc aagaccatat caccagtctg ctattttcca
    34621 aaaatgacta cttcctccgt caactccata aagagctaga acaccatgtc tattccgtgc
    34681 tagctgataa tttaaaagaa gcccacccta atctgcctac ttcttacctc caacacttgg
    34741 tcatgtccaa ctttatcgag acattgacct ggtggctcaa aaaaggtcaa gatttcacag
    34801 accaggaagt tgtccaattt tatctagacc ttctcattcc taaaaattga atatagagta
    34861 aagcttcagt tgtcttattt ctaggttact gagtttttta tcttttcaac aacaaaagag
    34921 gacccgccga tcctcttttt catactataa atccttgatt atcaactata tctgttttaa
    34981 tcgaaatctc aaaacagcac tttcaaacat cttttcctag ttaagtaaat cagtattctg
    35041 cttagctgcc ttgctccatt gataccaacc aactagactg ttaatgagat aaattagata
    35101 tttcccttga atttgcaggc tttctcccca ccagagatag attgaaaaga cattggtagc
    35161 cgcccagaat atccactgtt cacggtaaac agctgtcatg aggatttgcc ctaccccatt
    35221 ggttgcatct gtgattgaat cacgataggg acgattggca ccaatagact gataaatgaa
    35281 gccaaaggcc aaccaccaaa gcacactaat ggaaagatac tttgtccagc ccttgccgtc
    35341 cagtttacgc gcgacaaact cctgcttttc cttcttaaac tgtgcctgat aaatccaaac
    35401 tagaagtcca attggctgca tgactgtgaa gtaaagtgtc gtcagcacct caccataaaa
    35461 gcctttctgt agggccaaaa taaggtaaat aacagagtta atcaagccaa aaagataatt
    35521 acttgctcga ccttccgata caaagattac acagataatc cctgtcaagc tacaaatcat
    35581 cccaatccag tcaacaatac gatgttcgta aaccaactcc agccagagag gaaaacttcc
    35641 taaaaccagc aaataaatcc actgggcaaa actacgatgg gcaaagaggt catcccagat
    35701 agccttcata gttcctgaaa atcctaaatc agccatagcc gcaaccatac gacggtaacc
    35761 acctgacatt tcacctaggg ttgttttgat attttcaatt ttcttttgca aataagtatg
    35821 catcatttct ccttttgttt ttaaagagcc gtgtctggat agactttcgg acgcaacgct
    35881 ctattagata atgaactgcc tatacacaag atttctaacc ttagtcgaca tgagctgaaa
    35941 cctcttattt gttaagtagt tcacaaaata ttatacacct attttatgaa tagtcaactg
    36001 tctttacagt aaaattttag aaaatcatga aaattttctc tttctttcca ttttaagtga
    36061 cattcagtca ttctcacatc aaaaaagccc agacgaaatt gtctgagcat tcttttatct
    36121 agtcgtttaa ggaagttgag ttcagtatgt ttaaagtctc tgtcccatca tttcttcaac
    36181 aaaccttgtt cttggagaaa ctccttggct acttgctttg ctgacttgcc ttcaacaccg
    36241 acttggtagt tgagctggct catctggctt tctgtaatct taccagccaa tgtattaaga
    36301 actctttcca actctgggtg tttcttgaga agagcttctt tcatgagtgg agccccttga
    36361 taaggtggga agagttgctt gtcatcttcc aagacctgta aatcataacg ctccaattcc
    36421 gcatcagtcg aataggcatc cgtgatttga atatcccctg actgaatagc ctgatagcga
    36481 agggctggct caatggtcgc tacattgaga ttgagaccat acattgattg caagccctta
    36541 tttccatctt cacggtcgtt aaactcgagt gtaaaacctg ccttcaactg cccttccact
    36601 tttttcaagt ctgaaatggt cttcaagcca tattcttgag caatcttttt cggaacagct
    36661 acagcatagg tgttttgata agacatgggt ttgagatagg ctagatgatc ctgcttagca
    36721 atgccatcac gcgccacctg ataaacctgt tctggttcat gactcacctt gggtgatggt
    36781 tgaagcaaac tttcagtcac cgtaccagta aattcaggat agatgtcaat atcgcctttt
    36841 ttcagagctt cataaaggaa gcttgtcttc ccaaaattcg gtttaacagt cgcagtcatg
    36901 ctggtatttt cttcaatcag caacttatac atattggcca aaatttctgg ttctggacct
    36961 attttcccag caataaccaa gttttccttc tctttttgaa ccaaaagagc tggactataa
    37021 gacagaccca gtaataaagc caccaaggca aaacctgaga aaatcgtccg taattttgct
    37081 ttttccatca cttttagtag gaagttaaag gcaatggcta gcactgcaga agaaagtgcc
    37141 ccaatcaaaa tcaaactggc attattacgg tcaattccca aaagaataaa ggaacctagt
    37201 ccccctgcac caatcaaggc cgccaaggtt gccgtaccga taatcaaaac agctgccgtc
    37261 cgaatcccag acatgataac aggcatggcg agtggaattt caaatttctt gagacgttcc
    37321 catctggtca tcccaaaggc aatcccagcc tcttgcaggt tcggatcaat tcccttcagc
    37381 ccagtgatag tattttgcaa aatagggaaa atcgcataaa tcactagagc tgtcaaagcc
    37441 ggcaaggtcc caattcccat caaagggata aagagcccca acaaggccag agacgggatg
    37501 gtctggaaaa tacctgcaat ctgcaagacc cagtcggcca gcttctcatg atagcgaaga
    37561 aaaacagcca agggaatcgc aagcaaaata gctagtaaca aggtcaaaag cgacaactgc
    37621 aaatgttgag atagagctgt caaccaatca ctaaaacgat cctgaaaagt tgcaattaaa
    37681 ttagtcatga acactacctc caaacaagtc tgctacaaag tctgttgcag gcgcttttaa
    37741 aattgtctcg ggattcgcta cctggcgaat ttctccatcc tgcaagacag caatacggtc
    37801 cgccaacttc aaggcttcat ccgtatcatg ggttacaaaa atcgttgtca tcccaaactc
    37861 tttatgcaat tcttttgtca gaacctgcaa ctgttttctc gaaatagcat ccaaggccga
    37921 aaagggttca tccatgagga aaatcttggg ctgaccaatc atagctcgga caataccgac
    37981 ccgttgctgt tctccaccag ataattcact aggtaagcga tgcccatact cggctactgg
    38041 taaaccaacc ttagccaaaa gctcttctgt tttcttcgta atttcttcct tgctccaccc
    38101 cttcatttca ggaatgagag caatattttc cgcaactgtt agatttggaa aaagagcaat
    38161 agcctgtaaa acataaccag tagaaagacg aagttcacgc tcatcatagt ctttgatgcg
    38221 cttcccatcc atataaatat ttccatcagt tggttccaaa agacggttaa tcatcttgag
    38281 catggtcgtc ttacctgacc cagaaggccc tactaaaacc ataaattccc catcctcaat
    38341 ctgtaagttg acatctctca agacatcctt ttctgtgtag cgcagtgcta catttttgta
    38401 ttcaatcatt ctttgtcctc aatttaaaac ttccctcgat tggtcaagtc ttctacctta
    38461 ggcataactt ccttattatc ccaatgctcc acaattttcc cgttctctaa acggaagata
    38521 tcgtactggg cataagcaac gccatcaatc tgagtctgac catagctaac cacatagttt
    38581 ccttgtccta agagttggaa aacaaagtca aaagtgacac tatattcagc cacatagttt
    38641 ttataagcag cacttccttg tccaatatca tgattatgct gaatcaaatc gtctgccaca
    38701 taatcactcc actgctctag ctccccattt tggaaaattt ctgtcaagaa acggcgaacc
    38761 agctttttat tttctgcttt cttatccaaa tccttgattt caaaatctcc aaaaatttga
    38821 tctagttggt cattttcagg tgttcgatag tagtcaatga catcccaatg ctcaacaata
    38881 caaccattct catcctcacg gaaagtatcc gtcgtcaccc attgagcttc tccaccattc
    38941 agatattgat gaacatgaac aaagaccaga ttgccatcct caatggtgcg gacaatctta
    39001 atctgacgct ctggatgacg ctcaaagaaa tctgcaaaga aggctgcaaa tccttctttc
    39061 ccgtcaggaa cacctgtcga atgttggata taggtatccc ctacagactg ggcttgagcc
    39121 tcagcaactc gtccgtcttg aatggcatgg atgtataggt tgtgagcatt tttcacttgt
    39181 tgtgacatat tctaaacctc atttcccttc tctttcagat tcgccaaaat tctttcttga
    39241 aaaccttcaa attggtgaat ttcttcctct gaaaatcctt tgtaaaagat agtatccaat
    39301 ttctgactga cacgatgccc cacttctttc tgggacttgc ctaactccgt taaaactaaa
    39361 tacttcttac gcttgtcttt tccacacgga ctaacaatta caagcttttg ttcctctagc
    39421 ttttttatca tagtcgtcag cgtattattc gcaagtccag tcgcaagcgc gatatctgtc
    39481 gcagttgcgc agccagtttc actattccat aaaaccgcta aaatcttgcc ctgttcaccc
    39541 ctataaagag cctcaggatc ttgactcagt aacttttgaa aaatccgccc attcaacaaa
    39601 cgaatatgat gggctagcaa atgaccatct ttcataacac ctccaattta tttcgatatc
    39661 gaaatgaata aaacaattgt aacactcatc gttctaactg tcaactattt cgatttagaa
    39721 ataatttttg ataattatcc acaccaccat actccggctc aactaacttt taacgagagt
    39781 ttctaaactc cttcgtcctc cagtctacaa aagccttcca ttcgtactat cctatatttt
    39841 atgaggggac acatttttcc tatcagacca tttattttaa agatagaagt aaatcataat
    39901 tgcttccatc tgttctttta tagtatattg aagttagact agagcactgt atcttctaaa
    39961 acattgatag aaagcgattt gaatttccca atcaatttgt tcgtatttat agcatttcga
    40021 aactggaata ggacaccatg actgctaaaa gatttctata aattcattta atttcctcaa
    40081 tcaatttgtt catatcttat ttcattccgc tataatttca ccttacccta tctttttcgt
    40141 agcacccttc aaacagccta tcccctaccg tttgacgatt cctcacttcg ctccacttcc
    40201 attacagaag tttcttcact actatgggct cggctgactt ctcatgattc cttgttacta
    40261 ctatttgaac gctcacgaga tagatcttac aaaaaatgct ttgatccaca atggaatcaa
    40321 agcattttaa agagttcctc atacataagc gcagaagtcg cagttcctct gtacttggct
    40381 tcttctcttt tgacaaagcg agccaagttg agcaactcag gtgctggatg tttgggattt
    40441 aggagcaatt cacgattgac caggcctgag agacgaactg cctgcaattg ctcatttgta
    40501 gtaggcagtt ttttagtagt ctctaggaga gcagcaacta aatcttcact caaatcatgt
    40561 cgagcatgat tgtaaagatc ttttataagg ctttctaggt ttggttctac catccctacc
    40621 acctccctta tggtttaata atgtttaatc aaatcaaccg ttgaacgatc caatttcttc
    40681 accaaggctt gtaagaaagc ttgcgcttct aggaagtcat ccattgcata gagggtttgg
    40741 tgagaatgga tataacgagc gcagacaccg atagttgttg atgggacacc accatttttc
    40801 agatgagctg cacctgcatc tgttccgcct ttaccacagt agtattggta cttgatacca
    40861 gcttcttcag ccgttgtcaa aaggaaatcc ttcatccctg ggagaagcaa gtgacctgga
    40921 tcatagaaac gaatcaaggt tccatctcca atcttgcctt gaccaccgta gacatcacct
    40981 gctggtgagc aatcaactgc gaggaagact tctgggtcaa acttggttgt agaggtatga
    41041 gcgccacgca gaccaacttc ttcttggacg ttagaaccca gatagagttc attgccgagt
    41101 ttttgacccg ataaagcttc agctagctcg cttaccatga ggacaccgta gcggttatcc
    41161 caagcttttg agatgatatt tttttcattg gctgtcaaaa ttgcagaact atctggtaca
    41221 atggtatcac caggacggat gccaaaactt tctgcctcag ccttgtccgc aaaaccacca
    41281 tcaaaaacga tatcggcaat ggctggcatg gttggtcccc cctttccacg agtcaaatgc
    41341 ggaggaacag aacctgaaat cacaggaatt tcatgaccat cacgagtcaa gagtttgaaa
    41401 cgttggctgc taaccaccat ggggttccag ccaccgattt ctacgacacg gaaggtacca
    41461 tctggcttga tttcgctgac cataaaacca acttcgtcca tatgagaagc gaccaagacg
    41521 cgcggtgcat ccacagcttc tgaatgtttg ataccaaaaa taccacccaa gccatctgtc
    41581 accacttcat ccacatgcgg tgtcaacttt tcacgaagat aagcacggac aggcgcttca
    41641 tgacctgaga ctgcagcaag ttctgttact tctttaattt ttgaaaataa tgttgtcatt
    41701 tcagttcctt ctttctttca tccattttac cactttttat aggagaagga tagtgggaag
    41761 gtggatttct aagttagtat cttagtcctg ctctatctta gaaaaggata gtattctctt
    41821 gcatgtagtg caaaatctag taaacattcc aaaattaact cgaatattta tttccaaaca
    41881 aaaaaacaat acaccatcaa agttgtttgg atttttcatg aaatttacag aaaatagttg
    41941 acttcccttt cttctttctt taaatatata gttggttgag tttggaatag tacgctgtag
    42001 ctgctaaaac atttctagaa attaatttga ctttcctaat agagttgttc atatcttatt
    42061 tcaatttact atagtacaaa actagaaaag gaaaaaatca tgaccaaaac aattcttgtt
    42121 acaggtggga ctagctacat tggctcccat accgttaaag ctcttttaaa tgctggctat
    42181 caggtgcatg tcctcgataa tctctccaca ggaaatcgag cggctgtgga tagtcgtgct
    42241 agctttaaag aactggatgt ttatgatgct agcgccttaa aggcttactt agaagaaaat
    42301 cagattgatg ctgtcctcca ttgtgcaggt gaaattgttg tgagcgaaag tattgaaaat
    42361 ccaagtaaat acttcactgc caacgttgct ggtatgaacc aagttctcaa agtcttatct
    42421 gaagttggca ttcaaaaaat catgttctct tcgactgctt ctctctatgg aaataactgt
    42481 attgacaagc cggtgactga agataccctg ctcgaccctg tcaaccctta tgcagagaca
    42541 aaactgatgg gcgaacgaat gatttactgg atggccaatc gctacgactg gaaatatgtt
    42601 attttccgtt actttaatgt tgctggggct gaaatggatg cttcaaacgg tctgcgtgtg
    42661 aaaaatccaa ctcacatcat tccaaatatc aacaagaccg cattgggaca aaatgatagc
    42721 ttaaaaatat ttggagatga ctacgataca cgtgacggtt catgtattcg agattacatt
    42781 tatgtcttgg accttgcata ggctcatgtt aaaggaatga actacctatt tcaagaagac
    42841 agttcttctc aaatctttaa cttaggaact aaaaaaggct atactatcaa agaaatcttt
    42901 aaaactgctg aagaattact aaattaaaaa atcccacacg agattgttgc tcgacgtgct
    42961 agtgacccag ccagtgtcct agcagatgct tcaaaagcta aacaatatct tgattggacg
    43021 gctagctact ccctcgaaga tattatttta tcagattaag gtaaaaacat tttagaatag
    43081 gataacaaag gagaaagtct tgttgctctc tccttgttgt atttattaaa ttgtcagaaa
    43141 ttttattgac agattagaaa aataatgtta catttatatc cgcagatatc tttcgatacc
    43201 aaatctacat gaaaggacgg ggtatgaaac tttctcatta tttaattggc ttacttctac
    43261 tcctagtctt tctctctatt agcattggga ccagtgattt ttcatgggga aagctatttg
    43321 atttcgacca gcagacctgg ctcctctttc aagagtcccg tctcccaaga actatcagta
    43381 ttctcctgac tgcctctagt atgagtatgg caggccttct catgcagact attacccaaa
    43441 atcagtttgc tgcaccgagt acagttggaa cgactgaagc cgccaaactg ggaatggtgc
    43501 tgagcctttt tgtctttcca tcggctagtc tgacccacaa gatgctcttc gcttttgttt
    43561 catccatcgt attcaccctc ttcttcctag cctttatgac catttttact gtaaaggaaa
    43621 ggtggatgtt gcctctgatt gggatcatct atagcgggat tatcggctca gtcacagaag
    43681 ttatcgccta tcgtttcaat ctggttcaga gtatgactgc ctggacccag ggctccttct
    43741 ccatgattca gacccatcag tatgagtggc tcttcttagg cctcatcatc ctgataaccg
    43801 tttggaaatt atcccaaacc ttcaccatca tgaatctagg gaaagaaacc agcgagagtt
    43861 tggggatttc ctactcccta cttgaaaaac tggccctctt tctggtggcg ctaacgacaa
    43921 gcgtcaccat gattaccgtg ggtggcctac catttctcgg agttatcgtt cccaatcttg
    43981 ttcgcaagcg ctatggagat aatctaagtc aaaccaaact catggtcgca ctggttggtg
    44041 ccaatctagt tctggcttgc gatatcctat cccgagttct gattaggccc tatgagttgt
    44101 ctgtcagtct cttgctagga atcatcggta gtctcgtctt tatcctactt ctctggagag
    44161 ggggacgaaa agatgcagac taaaagcaaa cataccaagc tcttctggat tctcattatt
    44221 cttgccatcg gagcttgtct tctctacttt tggcccatca ctcacttgtc agcctttgct
    44281 tggaagttgc gttcccaaaa gatcatcgtt tatctcttgg tagccatcgc gactgggatt
    44341 tcgaccatta gttttcaaac cctgacggaa aatcgcttcc tgacgcctag tattttggga
    44401 attgaatcct tctacgtcct actacaaacc ctactactgg tttttgaaag caagtttctt
    44461 caacttggca aatcccctat cttagaattc ctagtcttac ttcttgtcca gtccctcttc
    44521 tttctcgcct tacaaggtta cttgaagaca ctgatgaagc aagacctggt cttcgtcctg
    44581 ctgatctgtc tagcgctcag aagtctcttt cgaaatatca gcaccttcct tcaagtccta
    44641 atggatccaa acgaatacga taaactgcaa aatagtcttt ttgcctcctt tcaacatctc
    44701 aacacttcca tcctagccat cggttctctg atcatcctcg ctttgacaat ctttttcttt
    44761 cgaaaagcag tcgttctaga tgtcttgcac ctgcaaagag aaacggctca gatattggga
    44821 ctcgatgttg aaaaagaaca gaaagagctc ctctggggaa tcgtgctttt gacctcaacg
    44881 gccactgcct tggtaggacc tatggccttc ttcggcttta tactggccaa cctcacctac
    44941 ctgattgtca aagactatca gcacaagtta ctctttatag tggccattct ggttggattt
    45001 attagcttaa ccttggggca agccttgatt gaacgagtct ttgcactgga aattcgtatc
    45061 agtatgatca ttgagagtgt gggtggcttc ttattcttta tcttactata taggaggtct
    45121 catcagtgaa actggaaaac attgacaaat ccattcaaaa acaggatatt ttgcaaggca
    45181 tttcgcttga agtcagtcct caaaaactga cagcctttat tggtccaaat ggtgctggaa
    45241 aatcgactct cctctccatc atgagcaggc taaccaagaa agatcaggga gttctcagta
    45301 tcaaaggacg tgaaatcgag agctggaatt cgcaagaact ggcccaagaa ctaaccatcc
    45361 taaaacagaa aatcaattac caagccaaat tgactgttga agaactggtc agttttggac
    45421 gttttcccta cagccgaggt cgacttagat cagaagactg ggaaaaaatc cgagaaactc
    45481 tgaactattt agaactgacc aacttaaaag accgctacat caatagcctg tcaggggggc
    45541 aactccagcg cgtctttatc gctatggtac tggcccagga tacggacttt atcttgctgg
    45601 acgaaccact caacaatctc gatatcaagc aaagcgtcag catgatgcag attcttcgac
    45661 gactggtgga ggaactcggc aagaccatta tcatcgtcct ccacgatatc aacatggcca
    45721 gtcagtatgc agatgaaatt gtcgccttca aggacggcca ggtctttagc aagggaagaa
    45781 ccgatcaaat catgcaggct gacctgctca gtcaacttta tgagattccc atcacgctag
    45841 ctgatatcaa tgacaaaaag atctgtatct atagctagta acataaaagc tcaagttaga
    45901 gaaccttcag tctcttagtc aataagatta agagactccc taaatcgtta tcacatttta
    45961 aaaaggagaa attatgaaaa catcccttaa actttatttc actgccctag tggccagctt
    46021 cttgctccta cttggtgcat gtagtacaaa ctcaagcact agtcagacag agaccagtag
    46081 ctctgctcca acagaggtaa ccattaaaag ttcactggac gaggtcaaac tttccaaagt
    46141 tcctgaaaag attgtgacct ttgacctcgg cgctgcggat actattcgcg ctttaggatt
    46201 tgaaaaaaat atcgtcggaa tgcctacaaa aactgttccg acttatctaa aagacctagt
    46261 gggaactgtc aaaaatgttg gttctatgaa agaacctgat ttagaagcta tcgccgccct
    46321 tgagcctgat ttgattatcg cttcgccacg tacacaaaaa ttcgtagaca aattcaaaga
    46381 aatcgcccca accgttctct tccaagcaag caaggacgac tactggactt ctaccaaggc
    46441 taatatcgaa tccttagcaa gtgccttcgg cgaaactggt acacagaaag ccaaggaaga
    46501 attgaccaag ctagacaaga gcatccaaga agtcgctact aaaaatgaaa gctctgacaa
    46561 aaaagccctt gcgatcctcc ttaatgaagg aaaaatggca gcctttggtg ccaaatctcg
    46621 tttctctttc ttgtaccaaa ccttgaaatt caaaccaact gatacaaaat ttgaagactc
    46681 acgccacgga caagaagtca gctttgaaag tgtcaaagaa atcaaccctg acatcctctt
    46741 tgtcatcaac cgtacccttg ccatcggtgg ggacaactct agcaacgacg gtgtcctaga
    46801 aaatgccctt atcgctgaaa cacctgctgc taaaaatggt aagattatcc aactaacacc
    46861 agacctctgg tatctaagcg gaggcggact tgaatcaaca aaactcatga ttgaagacat
    46921 acaaaaagct ttgaaataag ccacttcaaa ctcaatttaa tactcaatga aaatcaaaga
    46981 gcaaactagg aaactagccg caggttgctc aaaacactgt tttgaggttg cagatgaaag
    47041 ctgacgcggt ttgaagagat tttcgaagag tataaaattt tgacattcta aaacgcatcc
    47101 tatcaagttc actcaaacct gataggatgc gtttttatca ttttgaaatc tatttacccc
    47161 acctcattcc ccttcttgat ttcgtttaag ggaaaagcgg tctggaacgg ggtccttacc
    47221 tgttttcgac cagggatgac aacgtaaaat ccgagccaag cccatcaata cccccttaaa
    47281 cccatgtttt tcaatagcct gaatcatgta gttggaacaa gtcagctcaa agcgacaaga
    47341 gggtggaaag actggtgaga taaaacgttg gtaaaagcgc acaggcgcta ttaaaattcg
    47401 tttcattatt tcttagttac agccatggtg tgtagttggc tgatttcttt tttattaaga
    47461 cgacgggatt ctcctggacg gagtcctgtc aagtctaggt gtccgaaacg agtccgagac
    47521 aacttatcta cttggagacc aacagcttca aacatctttt taacctgatg gttacgccct
    47581 tcatggatgg tcaactgcac cacagagcga tttttgactg ggtccacttt gagaatttca
    47641 taaacagctg gcttggtttt cttaccatca atctcaagac cacgggtcaa ggggcggaga
    47701 ttgtccttat tggccacacc tttaacacgc gcgacataaa ccttgtcaat ctcattacga
    47761 gggtgaatca tctcgtctgt aaagtcccca tcattggtca aaatcaagac acctgatgta
    47821 tcccagtcca aacgacccac agggtaaata cgctctttga cattgggcaa gaggtcgaca
    47881 accgtcttgc gacccttatc atctgtcaca ctggaaatca caccgcgtgg tttgttaagc
    47941 agatagtaga ccttttcttc gttgtagata ggttgacctt caacttcgac cttgtcgcct
    48001 gacttgatag tggttgctag ttcacgcacc acttggccgt taaccgtcac caagccttgc
    48061 ttaatcagct cttctgcttt tctcctactg gccacacctg cgtgggcaat atacttattg
    48121 attctcatct tcttctatcc tttcaccaaa taattggctt tcttgggctt gaatctcaag
    48181 ctcatcaatc actggtaatt cttctaaatg gtttatcccc atgtaatcta ggaaataatc
    48241 cgtagtcaca tagaggttgg ggcgccccaa tacttccttt ttcccgtctt cctttatcag
    48301 gtcaaaagcc tgcaactttg ccaaggctcc actcgagtta actccacgga tggcatcaat
    48361 ttctatccgc gtaatcggct gtttgtaggc aataatggac aaggtctcaa gggcagcccg
    48421 agacaagctc tggttgatag gcgccttaga gtattccttc aaaatctctg caaattgagg
    48481 cttggtcacc aatctataag caccacttgt ctcaatcaaa gccaaactgg aatctgggtc
    48541 cttttcatac ttctgggcta attttcctaa actttgctgg atgcctgtcg gtggcagaga
    48601 gaggagttca gctaactggc ggacccgaat cccatcttca cccgctacaa acaagagcgc
    48661 ttctattttt gctaaagtac tcatctttcc tctctatcaa gtctagcttt gaggcacttg
    48721 actttcttcc ttcttttcca tgagatagat atctccaaaa ctctcctctt gcacgaggat
    48781 caactcctgg gttttgatta actctagggt tgccaaaaag agggtgatga cctcttggac
    48841 attctgggct tccttgaaca aatcctgcaa gcgcaattga tctcgtccaa tcaaggactc
    48901 tttcacgata atcatcatgt cctcaatctt atactcatcc cgcaagatcg tcgtgtgatt
    48961 ttgtgcaaac tcctcttttt tcttggctag gatatttgaa aaagtcaaaa agaggtcaat
    49021 ggtcgtcttg tcatgcacaa gctccgcatc ttcgtaaatc aactctgtcg gcgctttgga
    49081 ataatactgg gcccgttctt ggtgcttggc ttccaagtgc tcacccaaga gcttgaactt
    49141 gcgatattct tcgatttgag agaggaggtc ctgctccagg tcatccccca agtctgtcac
    49201 ttctgctacc ttcggaagga gtttacgact cttaatcagc atgagctgac tagccatgac
    49261 catgtactca cccgtcactt ccagacgcat ggcctgcagg gttgagacat aggctagata
    49321 ctgttcgatg acttccgtaa tgggcacatc gtagatatcc atctggtact tagaaaccag
    49381 atgcaagagc aagtccaggg gtccttcaaa atcttttaat ttaatatcca ttatctatat
    49441 ttttctaagg tcaggactgt ttttaatcct aatttttttg caatttcgta caaatcgacc
    49501 ttgttttcta tttgtcttag aataaactgt tcacgtaaga cttgagctga taaggatgga
    49561 aaacctttct ccttgacaaa agattctaac tgacgaaagg cccactgacg agaatagggt
    49621 ttctctcctc tttcaaaaag ataggtctgc cccatcaagg gttccaattc tgaaagcaag
    49681 gccgtgggaa tggtgacaat cctctgttgg gaagccttgc tgattcgcaa cacctgaaaa
    49741 tccagattga tgtccgcaac cttgatggct aaaatctcac tgggcaagag ccccatttct
    49801 aggattaaga gcgctagcaa gcggccctct ggatggtcgc tttcctgcca aaaagagtct
    49861 aggtatagaa tctctggctt ttccgtcttc ttttcagctt gtttggctaa ttccaagcgg
    49921 taaaagctgt ccacctctcc tttttgatag agaaagtata gaaattggtt acaggccgaa
    49981 atctttcgct tctgggcgct gatttttaga ttggctagct gggcttggta aatcttgaga
    50041 ctggtctcag aaatccgctc acctaccatg tctaaaaatt gctccaaatc atacttatag
    50101 gactgcttgg aattgacaga caagccctgc ttttcctcta aaaaggctga aatcctgtct
    50161 ctcattggca tcgaatctca tattccttac taaagtcatg caagagggca ttaacggcct
    50221 tgaggatgga cttgcgcgta ataatccctt ggaaaatacc ctctgcatcc acaaccggta
    50281 agaaggactc atctactagc ttgtgcaaga cctccgtaat ggtgaaatca ggcgaaacaa
    50341 ccgctacgtc cgtttttgtc atatgaacga tatccgtatc cgccatgatt tcttggctca
    50401 agtcatgctc catctgataa gccataatat ctctgagtcc aatcgtccca acaaactgtt
    50461 tttcatctgt cacaacggga acacgggtat aggtcatctg actgagcaag agggtcgcat
    50521 gatccgcatt gtgggtatca atcaacacag ctagattttt agcaggggtc aaaaaagttt
    50581 cctcctgccc caacaagaaa gtctcaaact ccttggcaat catcggctaa actccttgga
    50641 caaacctgga tacacctcgt gatctcgtgt caaaaagtcc actttgaagt aactatcatc
    50701 aatctccaca cgagcataga gacattctct gatggtacct cgtggttgac tgatagaacc
    50761 tggatttaga aagaggatct tgccttccaa ccaagcactt ggcacatgca agtgaccata
    50821 gaggcagata gcggcctctt cctcctgagc ccagtagtcc aacttttgaa agttgaaatt
    50881 gatgtcaaac aagtgaccat gagtttggat aatcttggtc gaaccaagct cagtcaccag
    50941 acgttctggg tagccggcgt agaagtccat gttcccttta acaacgcgga tgccctccca
    51001 aagtggagaa tccggacgta gttcagaatc gccgttatga aaaacagcat cgactttgcc
    51061 cacatagcga tcacggactt cttccacaat caagctatcg ccatgggaat cgctcattac
    51121 aatgatggtt tgctttgcca tgatggaaat acctccaaaa gtttcttaac ggctaaggca
    51181 cggtgagatt gactattttt ttcttccagg gttaattcag ctgatgactc acctgtttct
    51241 cctacaagga agaggggatc atagccaaag ccattttcac ccttaggttc aaagttaata
    51301 taacctgacc agtctgcttc aacaactaaa ctttccttat ttgggctggc tacgactagg
    51361 gttgtgtgga actgagccga gcggtccttg agttcaaaga ccatggccaa ttcgtgcaag
    51421 agtttggcat tattttcacg gtcagttgct cccacacctg cgaaacgagc tgaccagacg
    51481 cctggtaagc caccaaggac atcgactttg agaccagaat catctgccaa aaccatcttg
    51541 cccgttaatt gagaaatggt ttctgcctta aggcgggcat tttcttcaaa ggtcatacct
    51601 gtttctgcta cttcaggcag gtcagggtag tcattaagat tttccacatc gtagcctaac
    51661 ttatcaaaga tagctcggaa ttccttggtt ttaccttcgt tacgagtcgc aatcaacaag
    51721 gtttctctga ccttgcttgt ctcaaagaaa tcatgaacat tgaccccttc tttaggcaat
    51781 tctacataca agagttgccc attttcaacc aagagcaggg ccccatgacg ttgaataact
    51841 tccaagtttc gtcccatttc ttgattaagc atgtctacca caaaggagaa caaacggtag
    51901 atagaaccat agcgtaaaac agtaactgaa agcgggaaac catactcttc atctccaaat
    51961 attttggaga attcaaacag aggaaaatct gtcttatagt cgctcaaact gttaacgcca
    52021 ccaaaaatac tataagaccc aacataccag tcctggtcat ccttatattc ataaatttta
    52081 tttgtcataa ttctacatgc tccacatgaa tctctttttc cagccattct tcaccaattt
    52141 gtgcaaaact ttggctactg gctgttgtgt aaaaacggtg atggagtggt ccagcatcgc
    52201 gaccacgatt gatttcaaaa taattgagta agactgagat atcccgtacg cactctgccc
    52261 cactatcgat gagctgaacc tttggcccca tcacattttg gataataggg cgaaggagtg
    52321 gataatgagt acagcccaaa atcaggctat ccacctttcc aaccaaggga cgcagggttt
    52381 catagaccac cttcttggta acactggttg acagggcacc tgactcaacc aagggagcaa
    52441 acttgggaca ggccaagctc tccacctgta agtcgggatc cagatcatgg attttctgac
    52501 ggtatatgtc tgattgtacc gtcatgggcg ttccaatcac tccgattttc ccaccttgac
    52561 tggacttgat ggctgccgaa gctcctggca aaattacacc caagacagga atatctagtt
    52621 gagccttgat ttcttcccag acgaccgcag tcgcagtgtt acaagcaatg acaatcattt
    52681 tgacatcctt ggtcaagaga aagttgacca gctgccaagt atattcacga atttgctcag
    52741 caggacgggg gccatagggc gcccgcgccg aatctccaat atagacgatt tcttcatggg
    52801 gaagctggcg catgagctcg cgcacaacgg tcaagccccc gacacccgaa tccaaaaaac
    52861 caattggtcg attatccata cagtcttctt tctagtcttt ttttctgatt gatcgttcat
    52921 tatgattcct tgtactgaac gaactgaccg acagcttgat agaatgtcaa ctacctctct
    52981 cttaatcata atcaaatatc aatagaatgc aaggaaaaag tctcatccct aaaccataac
    53041 caacatagtg agaagtctgg aactttcatc ccagactttt ccttatttat tttttctttt
    53101 tattgttagc aagggctgcc ttttgttggc ggatgatttg gtggtaaact tgttgtacct
    53161 tagcttcgct tggtttttga ccatttgcac tcaaaagagt acgaactgct tcagcattca
    53221 aacgtgggtt gtcagcaaat tctttttcga tttgcttacg aaccaagtac attcctccta
    53281 gagctcctcc tagaaaagct agcacaatca atacaattgc taaaagtaaa tccatcattt
    53341 ttctcctgtt tctttttgag tccctttcat tataccacaa actagccacc ctgaccagtt
    53401 tgttctacac tttcagggat ggaatagaca gcaaaaagaa ccctgctttt ctgaaaaaga
    53461 ggtctccttg ctaagtttat actcaatgaa aatcaaagaa caaactagga agctagccac
    53521 aggttgctca aagcaccgct ttgaggttgc agataaaact gacacggttt gaagagattt
    53581 tcgaagagta ttaatttaca taaatagcca gtgtttgata gggtttgagt agaattttct
    53641 cagacacttc tgcatcttca tagtttgata tcaaaatctg tccattttgg tagactgctg
    53701 gcaagtcgat ttccacttct ttagcataaa agttattgag cactagtaac ttttgatcct
    53761 caaactggcg ttcaaaagcg tagacttgtt tgctatcttc aaaggctggt ttgtaacttc
    53821 cttctgaaat gattggcatt tccttacgca gtcgaatcaa gtcttgatag aaggtaaaaa
    53881 tcggaccctg gatttcattt tctacattga tgtatttata ggatttacca gctttcaacc
    53941 aaggagtgcc tgttgaaaat cctgcatttt ccgaagcatc ccactgcatg ggaatgcgtg
    54001 aattatcacg cgacttagct tgaataatct ggaaggcttc ttgctgactc tttccttctt
    54061 ctaagagcat ctgataggca ttaagcgatt cgacatccac ataatcagcc atagaatcat
    54121 agtctgggtc aatcatcccg atttcctcac ccatgtagat ataaggtgtc ccacgtgaca
    54181 ggtgaatgct ggctgctagc atggtggctc cttccttgcg gaagttttga atatcgacaa
    54241 aacggttcaa ggcacgtggt tgatcgtgat tattccaaaa gagggcactc caaccgtctt
    54301 tatcactcat ttccttaccc caactatggt aaagactctt caactcttca aaatcaaagg
    54361 gagccaaggt ccacttttgt ccatccttat agtccacctt gaggtgatga aaattaaagg
    54421 tcatggataa ttcctgacga tcaggcgacg aatagaggac acagttttcc atggtggtag
    54481 aagacatttc cccaactgtc ataaagctat cgtcggatcc aaaagtggct tggttcatca
    54541 tacgcaaata gttatgaacg atgggtttgt ctgtataagc tggcttccct tcattttcag
    54601 gacagtccac tgaaacctcg tccttaccga tcaaattgat cacatcaaat cggaaacctt
    54661 tgacaccctt gtcgcgccag aaattaacaa ccttgaaaag ctccttacgg acattggaat
    54721 tgcgccagtt aaggtcagcc tgggtctcat caaataggtg aagatagtat ttcccagtat
    54781 ccccgaaagg cgtccatgca gaaccaccaa acttagactg ccaatctgtt ggttggtctt
    54841 ggatgaagaa aaagtcttga taatacttat caccagctag ggctttctga aaccattcat
    54901 gctctgtcga acaatgatta agtaccatgt ccagcataaa gtcaatcttg tgctctttac
    54961 cgacacacac cattttctca aaatcagcca tatcaccaaa aagaggatcc actgccatat
    55021 aatctgaaat atcgtaacca ttatcccgtt gagggcttgg atagaatgga ttgagccaga
    55081 ccatatccac acctagtttg gctaaatagg gaattttttc gataatccca cggaaatccc
    55141 caataccgtt ttcagtggtg tctttgtaag attttggata gatttgatag actacttttc
    55201 ctttatcaag tgtcatctgt ttctcctttt ctgataaaag ggaggaagca gtcttccgtc
    55261 cctatttgtg ctatttcaat tatactcaat gaaaatcaaa gaacaaacta ggaagctagc
    55321 cacaggttgc tcaaaacact attttgaggt tgcagataga gctgacgtgg tttgaagaga
    55381 ttttcgaaga gtattagatt cgtgtagcga ccatgagaga tgctccagct tggatcgttg
    55441 tcggataagt tccgggaata gtcgctgtat aagcatcttg gttggtgatg ataacaggag
    55501 tttctgtcac cagacctgca gccttaatga catccatatc aaaacgaatc agttgctgac
    55561 caactgtaac gtgatctcct tggactacaa gactttcaaa acctttgcca tcaagaccta
    55621 ctgtatccat accgatgtgg atgagcaatt caactccctc gtcagagaca atgccgatgg
    55681 catgcttggt agggaaaaga accgtcactg tcccattaac tggagaggtc aactcacctt
    55741 ggcttggttc aatgactaga ccttgcccca tgacacctga tgcaaaaata ggatccgtcg
    55801 cttgactcaa ttctttcact tggccagtta gtgggctgat aatttctacc gaagtaagtt
    55861 ctactggttc atggttcaca aattctgctt cttcttgagc aacgaattct gcctgcaagt
    55921 tcgtatcgcc ctctgttttt gtaaagagac cagccttgcg gaagaagaaa gtcaagagca
    55981 ttggaacaac aatcgcaact agcatagttc ctgcaaatgg cagcatgtat tgaggttgaa
    56041 tagagagaat acctggcaaa ccaccgatac caatagaagc cgcagttaca ttaaaagtaa
    56101 cggataacat gcctgcaagg gctgaaccag tcatcccagc aacaaatgga taaatatatt
    56161 ttacgttaac cccaaaaaga gctggttctg taacaccgag ataggctgaa atggttgcag
    56221 gaagtgaaac ctgagcctca cgctcatcat ggcgatgcat gaaataatag gcaaacacgg
    56281 ctgagccttg agcaatatta gaaagagcaa tcattggcca tagggcagtg ccaccagcat
    56341 ccgcaatcaa ttgtgtatca atggcattgg tcatatggtg cagacctgtg atgacaaatg
    56401 gagcgtagag ggcgccaaaa attgcaccga agagccattt aactggacca gttaaacctg
    56461 ccaagacaac tgatgaaagt ccttgtccaa ttgtccaacc gattggtccc aaaacagtat
    56521 gagccaaaat caaggctgga atcaatgaca agaaaggtac aaaaatcata gaaatgactt
    56581 ctgggatatg cttgtgccag aagatttcaa gataagacag actcaaacct gcaagcaagg
    56641 ctgggataac ttgggcttgg taaccgatac gattaacagt aaaatagcca aaattccaaa
    56701 cccagtttgc cgcgatatca gctgctggcg ttgaagcaac cgcataggca ttgagcaact
    56761 gaggcgatac caaacagatt ccgagaacaa ttcccaaaat ttggctggtt cccatcttac
    56821 gagaaacaga ccaagtaatc cctactggta agaactggaa gatagcttca ccaggcaacc
    56881 agaggaagtg attgacacct gcccaaaact gagaggattc tgtgatggtc ttgccatcca
    56941 acatcgacca atggacacct tccaagacat tacggaaacc gaggatcaat cctccgacta
    57001 tcaaggctgg aataatcgga gtaaaaatct ccgccagagt ggtcataaca ccttggacca
    57061 cgttttgatt actcttagct gcagacttgg ctgcttcttt ggaaacaccc tcaatacctg
    57121 aaacggctgt aaaatcatta taaaagatgg gcacgtcatt tccaatgatt acctgaaatt
    57181 gacctgcatt tgtaaaggtt cctttaacag ctggaattga ctcgatagct ttaacattag
    57241 ccttcttatc atctcctaaa acaaaccgca tccgtgtcgc acagtgagtt acggcagtca
    57301 cattttcttt gcctccgatt gcctgaagca gatctttggc ttcttgttca aattttccca
    57361 tttgattctc cttatacttg tcaaagcaag tttgatttga taagtatatt gtaaacgata
    57421 ccaaacttgt ctgcaacttg tttggtataa aaagggtgtt tttttgtaaa ttttctagca
    57481 cttgtatggt aaaatagata caggtgttca ttaaactaga ctaaaaacct atttaagcag
    57541 gcaaaatgaa gaaataccaa caattattta agcaaatcca agaaaccatt caaaacgaga
    57601 cttacgctgt cggagatttc cttcctagcg agcacgacct tatggagcaa tatcaagtga
    57661 gtcgtgatac cgtccgaaag gccctgtctc tcctccaaga ggaaggattg atcaaaaaga
    57721 taagagggca aggttctcaa gtcgtcaaag aagaaaccgt caatttccct gtatccaacc
    57781 taaccagcta ccaagaacta gttaaagaac ttggactgcg ctctaaaacc aacgtggtca
    57841 gtctggacaa gattattatt gataaaaaat cctcactgat aaccggtttc ccagagtttc
    57901 ggatggtttg gaaggtggtc cgccagcgtg tggtggatga tctggtatcc gttctggata
    57961 cggactatct ggatatggaa ctcatcccaa atctcactcg ccaaattgct gagcagtcta
    58021 tctattctta tatagaaaat ggcctcaaac tccttattga ttatgctcag aaggaaatca
    58081 ccattgacca ctcaagcgac cgagacaaga ttctcatgga cattggcaaa gacccttatg
    58141 tcgtttcgat taaatcaaaa gtctatctcc aagacggacg ccaatttcag tttaccgaaa
    58201 gtcgccataa gttagagaaa tttagatttg tagattttgc aaaacgcaag aaataaaaga
    58261 ctgagacacc agatctcagc ctttttcggc tctataatat ttgtagtggg taacccccct
    58321 atggatatta tggagcctat ttt
//
LOCUS       DBDESG010000003        57099 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.003, whole genome shotgun sequence.
ACCESSION   DBDESG010000003 DBDESG010000000
VERSION     DBDESG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 57099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.003"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(10..726)
                     /gene="ldcB"
                     /locus_tag="ACKB38_000187"
     CDS             complement(10..726)
                     /gene="ldcB"
                     /locus_tag="ACKB38_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGL7979041.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(729..1199)
                     /locus_tag="ACKB38_000188"
     CDS             complement(729..1199)
                     /locus_tag="ACKB38_000188"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGL7979042.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(1201..2238)
                     /locus_tag="ACKB38_000189"
     CDS             complement(1201..2238)
                     /locus_tag="ACKB38_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGL7979043.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            complement(2336..3661)
                     /gene="brnQ"
                     /locus_tag="ACKB38_000190"
     CDS             complement(2336..3661)
                     /gene="brnQ"
                     /locus_tag="ACKB38_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGL7979044.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(3832..4119)
                     /locus_tag="ACKB38_000191"
                     /pseudo
     CDS             complement(3832..4119)
                     /locus_tag="ACKB38_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(4279..4857)
                     /locus_tag="ACKB38_000192"
     CDS             complement(4279..4857)
                     /locus_tag="ACKB38_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGL7979045.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQVLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(4911..6311)
                     /gene="pepV"
                     /locus_tag="ACKB38_000193"
     CDS             complement(4911..6311)
                     /gene="pepV"
                     /locus_tag="ACKB38_000193"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGL7979046.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FHFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(6324..6929)
                     /locus_tag="ACKB38_000194"
     CDS             complement(6324..6929)
                     /locus_tag="ACKB38_000194"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGL7979047.1"
                     /translation="MKFLELNKKRHATKHFTDKLVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            7100..7900
                     /locus_tag="ACKB38_000195"
     CDS             7100..7900
                     /locus_tag="ACKB38_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7979048.1"
                     /translation="MKKFSLLLAILPFLVACENQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(8082..8915)
                     /locus_tag="ACKB38_000196"
     CDS             complement(8082..8915)
                     /locus_tag="ACKB38_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGL7979049.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRSQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(8905..10749)
                     /gene="uvrC"
                     /locus_tag="ACKB38_000197"
     CDS             complement(8905..10749)
                     /gene="uvrC"
                     /locus_tag="ACKB38_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGL7979050.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(10789..11490)
                     /locus_tag="ACKB38_000198"
     CDS             complement(10789..11490)
                     /locus_tag="ACKB38_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGL7979051.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(11569..12801)
                     /gene="murN"
                     /locus_tag="ACKB38_000199"
     CDS             complement(11569..12801)
                     /gene="murN"
                     /locus_tag="ACKB38_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGL7979052.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(12806..14026)
                     /gene="murM"
                     /locus_tag="ACKB38_000200"
     CDS             complement(12806..14026)
                     /gene="murM"
                     /locus_tag="ACKB38_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGL7979053.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(14138..14917)
                     /locus_tag="ACKB38_000201"
     CDS             complement(14138..14917)
                     /locus_tag="ACKB38_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGL7979054.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(14986..16647)
                     /locus_tag="ACKB38_000202"
     CDS             complement(14986..16647)
                     /locus_tag="ACKB38_000202"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGL7979055.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(16822..19044)
                     /gene="recJ"
                     /locus_tag="ACKB38_000203"
     CDS             complement(16822..19044)
                     /gene="recJ"
                     /locus_tag="ACKB38_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGL7979056.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            19321..20079
                     /locus_tag="ACKB38_000204"
     CDS             19321..20079
                     /locus_tag="ACKB38_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7979057.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            20119..20883
                     /locus_tag="ACKB38_000205"
     CDS             20119..20883
                     /locus_tag="ACKB38_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGL7979058.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            20896..21573
                     /locus_tag="ACKB38_000206"
     CDS             20896..21573
                     /locus_tag="ACKB38_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7979059.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            21583..22242
                     /locus_tag="ACKB38_000207"
     CDS             21583..22242
                     /locus_tag="ACKB38_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7979060.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIVLQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            22500..23690
                     /locus_tag="ACKB38_000208"
     CDS             22500..23690
                     /locus_tag="ACKB38_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGL7979061.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(24011..24892)
                     /locus_tag="ACKB38_000209"
     CDS             complement(24011..24892)
                     /locus_tag="ACKB38_000209"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGL7979062.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(25033..26361)
                     /gene="vncS"
                     /locus_tag="ACKB38_000210"
     CDS             complement(25033..26361)
                     /gene="vncS"
                     /locus_tag="ACKB38_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGL7979063.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(26358..27014)
                     /gene="vncR"
                     /locus_tag="ACKB38_000211"
     CDS             complement(26358..27014)
                     /gene="vncR"
                     /locus_tag="ACKB38_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGL7979064.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(27063..27146)
                     /gene="pep27"
                     /locus_tag="ACKB38_000212"
     CDS             complement(27063..27146)
                     /gene="pep27"
                     /locus_tag="ACKB38_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGL7979065.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(27326..28705)
                     /gene="vex3"
                     /locus_tag="ACKB38_000213"
     CDS             complement(27326..28705)
                     /gene="vex3"
                     /locus_tag="ACKB38_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGL7979066.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(28757..29404)
                     /gene="vex2"
                     /locus_tag="ACKB38_000214"
     CDS             complement(28757..29404)
                     /gene="vex2"
                     /locus_tag="ACKB38_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGL7979067.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(29417..30694)
                     /locus_tag="ACKB38_000215"
     CDS             complement(29417..30694)
                     /locus_tag="ACKB38_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979068.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(31051..31221)
                     /locus_tag="ACKB38_000216"
                     /pseudo
     CDS             complement(31051..31221)
                     /locus_tag="ACKB38_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(31364..31510)
                     /locus_tag="ACKB38_000217"
     CDS             complement(31364..31510)
                     /locus_tag="ACKB38_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979069.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            complement(31507..31962)
                     /locus_tag="ACKB38_000218"
     CDS             complement(31507..31962)
                     /locus_tag="ACKB38_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979070.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            complement(32099..32983)
                     /locus_tag="ACKB38_000219"
     CDS             complement(32099..32983)
                     /locus_tag="ACKB38_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7979071.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(32987..33373)
                     /locus_tag="ACKB38_000220"
     CDS             complement(32987..33373)
                     /locus_tag="ACKB38_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGL7979072.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(33366..34709)
                     /gene="cysS"
                     /locus_tag="ACKB38_000221"
     CDS             complement(33366..34709)
                     /gene="cysS"
                     /locus_tag="ACKB38_000221"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGL7979073.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(34791..35675)
                     /locus_tag="ACKB38_000222"
     CDS             complement(34791..35675)
                     /locus_tag="ACKB38_000222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7979074.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(35687..36304)
                     /gene="cysE"
                     /locus_tag="ACKB38_000223"
     CDS             complement(35687..36304)
                     /gene="cysE"
                     /locus_tag="ACKB38_000223"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGL7979075.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(36320..38533)
                     /gene="pnp"
                     /locus_tag="ACKB38_000224"
     CDS             complement(36320..38533)
                     /gene="pnp"
                     /locus_tag="ACKB38_000224"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGL7979076.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            complement(39218..40084)
                     /gene="metF"
                     /locus_tag="ACKB38_000225"
     CDS             complement(39218..40084)
                     /gene="metF"
                     /locus_tag="ACKB38_000225"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGL7979077.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(40148..42397)
                     /gene="metE"
                     /locus_tag="ACKB38_000226"
     CDS             complement(40148..42397)
                     /gene="metE"
                     /locus_tag="ACKB38_000226"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGL7979078.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(42658..42924)
                     /locus_tag="ACKB38_000227"
     CDS             complement(42658..42924)
                     /locus_tag="ACKB38_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979079.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(43050..43465)
                     /locus_tag="ACKB38_000228"
                     /pseudo
     CDS             complement(43050..43465)
                     /locus_tag="ACKB38_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            43621..44622
                     /locus_tag="ACKB38_000229"
     CDS             43621..44622
                     /locus_tag="ACKB38_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGL7979080.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(44690..47095)
                     /gene="pheT"
                     /locus_tag="ACKB38_000230"
     CDS             complement(44690..47095)
                     /gene="pheT"
                     /locus_tag="ACKB38_000230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGL7979081.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIVSLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(47172..47681)
                     /locus_tag="ACKB38_000231"
     CDS             complement(47172..47681)
                     /locus_tag="ACKB38_000231"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7979082.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(47681..48727)
                     /gene="pheS"
                     /locus_tag="ACKB38_000232"
     CDS             complement(47681..48727)
                     /gene="pheS"
                     /locus_tag="ACKB38_000232"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGL7979083.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(49209..50624)
                     /locus_tag="ACKB38_000233"
     CDS             complement(49209..50624)
                     /locus_tag="ACKB38_000233"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGL7979084.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(50637..52475)
                     /locus_tag="ACKB38_000234"
     CDS             complement(50637..52475)
                     /locus_tag="ACKB38_000234"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGL7979085.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(52493..53332)
                     /gene="licT"
                     /locus_tag="ACKB38_000235"
     CDS             complement(52493..53332)
                     /gene="licT"
                     /locus_tag="ACKB38_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGL7979086.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(53631..55325)
                     /locus_tag="ACKB38_000236"
                     /pseudo
     CDS             complement(53631..55325)
                     /locus_tag="ACKB38_000236"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(55428..56293)
                     /locus_tag="ACKB38_000237"
                     /pseudo
     CDS             complement(55428..56293)
                     /locus_tag="ACKB38_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            56841..57099
                     /locus_tag="ACKB38_000238"
                     /pseudo
     CDS             56841..57099
                     /locus_tag="ACKB38_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT        16536 a        12887 c        10367 g        17309 t
ORIGIN      
        1 cgaagagtat taatcgacgt agtctccgcc ttcaaagcca tagtattctt ccaaactgag
       61 accacttgca gcaatttctt tagcttcttt tcctacataa cgcaggtgcc attcttcagc
      121 catatagcct gtttcctttt ccttgccttt gagataacgg acaacaaagc cataatcagc
      181 tgcatgatcc aagagccatt gggctgcttt ttcttctgtc accaaatcac catcagtccc
      241 aatcacatca aaggccaagc ctgtctggtg ttcgctatag ccaggacggg cagagtaacg
      301 gtcagctgct gcctttccat cttggttgac ataatcttga tagagcttgg tctgagtttc
      361 ataacttcta aaaccactgt aatgatcact aatagggaaa cctgcctctt gcatcgcttt
      421 gatgagtttg accaactctg ccttggctgt tggattttcc cctggattat agtctttaga
      481 caatggatag tgtttgttgg ctacgatgat ttcatcgtat ttcccttgaa tgctgtagta
      541 atcaccttta ttgaccactt ctgctttctt ctgggcagct ccttgagact tactaccgac
      601 tgttccatca gctttggctg tctgttctgt cttagtttct ccatcttcat tttttgtttt
      661 ttcttgtgaa caagctgcta gactcaaggc tagcaaagct gtcaagacta ggtatctttt
      721 cttcatttct actcctttat ttctaatagt ttatctactt ctgacgataa acgtttgact
      781 aatcttttaa tctcatccgg ttttgcttgg caggtttctg ctgtcatttc ttcaaaggga
      841 acccaccaga ctggtccacg acccttgaga ccatgaccat tcatatcacc tgtccgtcgt
      901 ggaaacagat gccaatgaag atgagcatcg ccatttccta gcagttcgat attcattttc
      961 tcagcagcaa aggccttggc aactgcctct tggactaaac tcatttcttc tagaaaacgg
     1021 agtcttgtct cctttttcaa atggtgcaat tcgctgacat gctccttggc tagaaagaga
     1081 ctatagcctt caaaatactg gtggtctcca accacaagat agcctgtttc caactctttg
     1141 acaaagtaag gattttcttc cttcttgatg aggtcaattc tctgacaaat aaggcacata
     1201 ttagtccacc aacgtgctct taagataagc atcgaccatt cgttctgttg cgatcaccga
     1261 gtcaatatgg gtacgctcat aggaatggct agactctata ccagcaccga gaagggcgtg
     1321 tttgacttct gcccctgcag acatagccgc tgaagcgtcc gaaccataaa atggatagat
     1381 atccagctta aatggaatat cttgctcttt cgccaaagcc accaaatgtt gacggaagtc
     1441 atagtgataa ggtccagaag catccttgac acagatagac actgtatatt cgtctgtttg
     1501 ctggtcatct cccatggctc ccatatccac agccagatat tctactacct gagcaggaat
     1561 gttagagttt gcaccgtgtc ccacttcttc aaagactgaa aaagcaaaat gagttgttac
     1621 gggcaattca atcttctctt ccttataaat gcgaaggaga ttgagcaaaa tcgccgcact
     1681 gaccttgtca tccaaatggc gagacttgat aaaacctgtc tctgtcacga cagttcgtgg
     1741 gtcaaaactg ataaaatcac cgacctcaat gccaagagca cgagtttctt tttcactagt
     1801 tactttggcg tccaaacgca cttccatatt gtcttgcgtg cgttctgcag ttcctgcatc
     1861 cttatagaca tggcaagaag tttggtggat gaggatggtt cctgatactt tttcacctgt
     1921 gctagccaca tgaatggtac agttttctcc ttcaatcatg ttccaaggaa agccaccgat
     1981 acggtccatt ttgagacggc cgtctggttt gacagcacgg acaatagcac caagcgtatc
     2041 tacatgggca gtcacatagc gatgttgctc atcattttga cctttaatag ttacattgac
     2101 accgcccttg gatgtgcgaa ccggctggta accaaaacct tctagagtct tgactaaata
     2161 gtccgcaatc tcacgagtaa agcctgttgg cgacgcaatg gctgtcagtt ctttgatata
     2221 ttctactgtt tgattcattt cttcacctct ttcgctacct attataacac atttatcatc
     2281 ggataaaaaa agaaaatcac tcctgtagac ttggctacaa aagtgattta agtgattatt
     2341 ccatttcaaa aacatcgctt tcttgcttgt ttggtagaac caatgagagc aagattccaa
     2401 caatggctgg caccaaccat gggagagatg ccttggcaaa aggaagaacg ctaacaagat
     2461 ttgcaagaaa ctcaacctta aacgagcttc ctagtacgct tgcaatggca ataactgtaa
     2521 ccacagcaat tgtcaactgc atacctggtt ttgaaagggc aacaaatttg ttgacaatga
     2581 caatcataac gatagcaatc gtgattgggt acaagataac cagtactgga attgagtact
     2641 tgataatcgc atcaagaccc aaattggcaa tagcaaatcc aatcaaggta aaggctgtcg
     2701 cataaacctt gtagctgatt tgtgggaagc gctcattaaa gaactcagct gttgacacaa
     2761 tcaaaccaac agtcgttgtg aagcaggtta cggtaaccat agctgcaagg aagagttgag
     2821 ctgttgagcc aaagatttct tgagtggctt gtgacaagat gtaaacacct ggtgttccac
     2881 ccttcatcgc ttcagctggt actgggaaat gatttccaag aaaacctaaa ccgatgtaaa
     2941 gagcgctgaa ggcaagggca acaacgatac caacaaccca aatagttgaa atgtattctt
     3001 tcttacttga aaatccaagt tgtttcaagg tttgaactgc gattacgcta aaggccactg
     3061 aggcaagggc gtccaaggta ttgtaacctt ctaggaaacc tgtaccaaag gcagaagctt
     3121 gataagcagc tgaagcagct tgaggacttg ttccaccata tttgatagct cccagaacga
     3181 ccaagataac aatcaaaatt gcaaagactg gcgttaaaat acgtccaatg cggtctaaga
     3241 tttttgatgg attaagcgaa atcaaatagg ctgccgcaaa atacagaacc gtaaatacaa
     3301 tcaagccaag tcctttattt gcatccgaca aaagggggct aatccctact tcgtaagctg
     3361 ttgtagcagt acgtggggta gcaaagaatg gaccgattga caagtaaaga actgagaggt
     3421 aaagagtcgc aaaccaaggc gctatcttcg ttgaaatctc gtagatatat cctttaggat
     3481 ttagcgttcc aataataagg gtcaagacgg cgataccaac gcctgaaaag acaaaacctg
     3541 cgatggcagg aagaaaatgt tctccagata gagcacctag agaaggcgga aaaatcaagt
     3601 tccccgcacc aaaaaatatt ccaaacagga gcaaacctgt tagggtacct tttttagcca
     3661 taaaaatctc cttcatattt ataaaatact gacctagtat accacgatta ggagtctgaa
     3721 aaaagcatca cgaggcaaat attaaatgtt ttcaattttc ttaaaaataa gaattatcta
     3781 aaaataaaac tctcctaccc agtccacacc aagtaggaga aatgcaaaat tttaaagttc
     3841 ttcaaaggct aacatgccac cctggacatt gataacatta taaccttgtt ctaataggaa
     3901 ttggcaagca cgcgccgatc tcattccaga tttgcaaata atataatgca agcgatcttt
     3961 gtccaattaa tcataactat cagccaattg actaagcggt aggttgtggg caccttctaa
     4021 atgaagagct gcaaactcat ccacttctct cacatccact aaagaaagct ggtcgttttg
     4081 gtaaagctgg taaaatgcgt caaaggctat ttctttcatt ctttttcttc taatactctt
     4141 cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgtg cgtatgttac cgacttcgtc
     4201 agttttatct gcaacttcaa agctgtactt tgagcagcct acgactagct tcctagtttg
     4261 ctctttgatt ttcattgact ataaaatggt tttaattctt tttttcaaat ctggcactac
     4321 ttctgcctca aaccaaggat ttttggccat ccagatttga tttcgtggtg atgggtgaac
     4381 tagcggaaaa taggctggca gatagtcttt atagtgtttc accctctccg ttaccttccc
     4441 actgattttc tcctgtaaat agtaggcttg ggcatattgc ccaatcaaga gggttaactg
     4501 aatatcaggc aattcctgta agacctgcgg atgccatttt tctgcaaaac ctgtacgagg
     4561 cggaagatca cccgacttgc catgtcctgg aaagtagaaa tccataggca aaacagcaaa
     4621 ataacctgaa ttgtaaaagg tatcttcatc cacacctagc cagtcccgca agcggtcacc
     4681 acttttatct ttccagtaaa ggcctgcttc ttgagtttta agtcccggag cctgaccgat
     4741 gatattgatg cgagcagttt ttggcgctgc aaagagaggc tcaatgccac gctctgtata
     4801 gctggcattc tgcgaatccg ccatgatagc ctgtttgatt ctttcgattt gagacatcta
     4861 ctttccctcc aaaaagaagt ccaagcataa gatctcagac ttctatcgtt ttatttgatc
     4921 aattcgtaaa tagcttcggc ataaattgct gctgctcgga aaagatcatc caaggcgata
     4981 aattcattgg cttggtgcat ggtatcaatc gagtctggga acatagcacc gtaggcaact
     5041 ccgcgttcta gcaagcgacc aaaggttcca ccaccgatga cttgttcatg acctttaaag
     5101 ccagtttgtt tttcatagat attcaacaag gtttgcacaa gtggatcttc cattggcaca
     5161 tagtgaggcg tgtgaccgtg ttcagacagg ctaacagaaa caactggcaa gttttcaagg
     5221 attgacttga tttgttctgg acttgttcct tttggatagc ggatgttgag ggcaatggta
     5281 ttatcagcac ttgtttcatc gaagtggaag acgccggcat tcatagaaag agcacccatc
     5341 ttttcatcca catgagcaat cttaagattt tcaccctcat gatcgttcaa gagaatttta
     5401 cctgcgatgt caaggtagtc tttggctgga ccagcaaagc caaactggct gaggaagagg
     5461 gcaaggtaag ttgcgccatt gacacctgaa gcaggcatag caccgtgggc tgatttacca
     5521 atgatcgtca ccttgtattt gccagcttct tcttggagtt ctcctctaag tttgtgttct
     5581 gcaacaaagg catctagttt agcttgcaag tcagccaagt cacctgaaac gactgctgtt
     5641 gctgattctg gtaccatatt ttcacgtaaa ccacctgtaa agctgtgaag acgggcaaca
     5701 cctgtatttt ctcctgcaaa gtggaggtat tccgtgatat ttcctttttc accattgatg
     5761 attggaaatt cagcatctgg tgagaaaccg aaatctggtt tggcaagtcc tacgtgctca
     5821 aagtagtagt ccatgtctgc ccagcctgat tcttcgtctg ttccaacgat gaagcgaact
     5881 ttcttagaag ttggaagacc caattctttg atgattttca aaccatagta acaagctgtt
     5941 gtaggaccct tatcgtccga agccccgcgc gcataaaggc gaccatcttt gatagttggt
     6001 gtgtaagggt ctgtgtccca accgctacca gcaggcacca catccatatg ggcaaagatt
     6061 ccgagaactt cttctccatc accaaactca aaatgtcctg catagttatc aacattctta
     6121 gttgggtagc catcgcggtc tgcgatttca aggaatttct ccaaggcttt tactggacca
     6181 ggcccaaatg gatgctgggc atcagccttg ctgtcatcac gttctgaatt gatttccaaa
     6241 aggctaaaca agtcagccaa gaggtcttct ttgcgttttt ctacttctgc tgtaaaatca
     6301 attgctgtca ttttttcttc tttctatctt ttctcgatga tttcatctac tggcaagcgg
     6361 tagcttggtt ccaatttttc gtctgtataa cccactgtga tcaagagttc tgggcggaaa
     6421 cggtcttcga tttccaaaac ttcattaact tttgatttgt caaaaccaag aataatgtta
     6481 gaaccaattc cttggtctgt caatgcaaga accaagttca tggcaaccaa acctgcattg
     6541 agagctaggt agtcgctgac ttgttgctca ctgtaacggg caaactcagc tggcagattt
     6601 ttcataaaat attgaagttg ctcttcagaa aagttattag caccaccaac acgggcaatc
     6661 ttacgagcac gtttggctaa gtccgtatct gtaaacaagg caatggttac aggcgctgat
     6721 gatacctgtt caaaattgga accataagct aactttgcca gttcagcatt tttctcacgt
     6781 accaccacaa atttccaagg ctggctgttg tgggcgcttg gcgccaaggt tgcaatttcg
     6841 atagccgtac gcacatcttt gggatcaaca agcttatcag taaaatgctt agtcgcatga
     6901 cgttttttat ttaattcaag aaatttcata atcgatttcc ttttctaatt ctactgcttc
     6961 cattctacca taatttgaaa gagcttgcag ggatttttca tgattaagtt tttctatcac
     7021 agacataaaa gatatatatt ggtttatcag gaaagtttct gataaactag caagtacttt
     7081 acatttgaat ggagacagta tgaaaaaatt tagcctatta ctagctatcc taccattttt
     7141 ggttgcctgt gagaatcaag ctacacccaa agagactagc gctcaaaaga caatcgtcct
     7201 tgctacagct ggcgacgtgc caccatttga ctacgaagac aagggcaatc tgacaggctt
     7261 tgatatcgaa gttttaaagg cagtagatga aaaactcagc gactacgaga ttcaattcca
     7321 aagaaccgcc tgggagagca tcttcccagg acttgattct ggtcactatc aggctgcggc
     7381 caataacttg agttacacaa aagagcgtgc tgaaaaatac ctttactcgc ttccaatttc
     7441 caacaatccc ctcgtccttg tcagcaacaa gaaaaatcct ttgacttctc ttgaccagat
     7501 cgctggtaaa acaacacaag aggataccgg aacttctaac gctcaattca tcaataactg
     7561 gaatcagaaa cacactgata atcccgctac aattaatttt tctggtgagg atattggtaa
     7621 acgaatccta gaccttgcta acggagagtt tgatttccta gtttttgaca aggtatccgt
     7681 tcaaaagatt atcaaggacc gtggtttaga cctctcagtc gttgatttac cttctgcaga
     7741 tagccccagc aattatatca ttttctcaag cgaccaaaaa gagtttaaag agcaatttga
     7801 taaagcgctc aaagaactct atcaagacgg aacccttgaa aaactcagca atacctatct
     7861 aggtggttct tacctcccag atcaatctca gttacaataa cactcttcga aaatctcttc
     7921 aaaccacgtc agctttatct gcaacctcaa agctgtgctt tgagcaacct acggctagct
     7981 tcctagtttg ctctttgatt ttcattgagt ataagatata ctaaaagcga ttggaccagc
     8041 caatcgcttt tttagtttgc attggctatt ttaggaaact cttacaaaaa atcaaaaaaa
     8101 ttcttcacat cctctacata cccatgcttt tctattaagc tggctaataa ttccagattg
     8161 tgcccctgat agttatcgtc acgacgcagt tcttcataca tttcgataaa gggaagcccc
     8221 ttggacttgg ccaattctaa ctcagcttcg taatcataag caactttacg aaattggata
     8281 ttgagtaatt ccccatcttc aacttctatc acggcatact gggaacggtg attttttaac
     8341 gcctcccaat taaaataggg catgccaatc gaccctggat tgatgatttg ttgcccttga
     8401 cttccataac gaagcaactg cttgtgaaca tgaccataaa ctgccacgtc cgtttccgca
     8461 tctagcagtt ggtcaaattt ctctgtatca ttctcaacta gcaagtcacc accatagttt
     8521 ttgtcaggta aattatgaga gatagaaaag cgcaatccgt caatttcttt cttttccagc
     8581 aaaggcaagc ttcgtagcca gacaatcgtt gcaggatcca ttcgctccat caaatactgt
     8641 gtcatacgca agagctgaac ttcctgtggg tcttctaagc catattgccc atctaaagcc
     8701 tcaaggacac gatcatccca attgcctcga acacttgctg tgataggaag gtcctttagc
     8761 agggcgacta agtcatttgc gcctggacca ggaagaaaaa tatctcccag aagccaatat
     8821 tcactggccc cttgattttt agcatctgca atcactgctt ctagcgccgt cgcattgcca
     8881 tgaacatctg ataaaattgc gattttatgg ttcattgtgg tttccttccg tttggtaatc
     8941 tactctttct tctgctactt gaggcaaggc ttctccctgc gggttcaact ttctttgcac
     9001 agcctctgca acgactctag gtaccccaac ttcgacaatc tcatccacac tggcttcctt
     9061 gattttggtc aaagacttga aatgcttcat aagattctgc ttgcgtttag gtcccagacc
     9121 gtcaatccca tccaattgag atgagaaaga atttttggag cgcagttggc ggtggaaagt
     9181 gatagcaaag cggtgcacct catcttggat gcgttggagg aggaaaaatt cctgagaatt
     9241 gcgagacaaa tccaccacct caagcggatc tccaaagagc aattcatggg tttggtgctt
     9301 atcattcttt tgcagcccag caattggaat atccaagccc agttcctctt ggataacctg
     9361 cttagcgata ttgacttgac cttgcccccc atcaatcaca atcaaatctg gaggagtcaa
     9421 agcctcacgc tgtactcgac catagcgtct gcgaatgacc tctctcatgc tggcatagtc
     9481 gtctggtcca acaaccgttt ttatcttgta cttacggtaa tccttcttac tcggtttacc
     9541 gttgacaaag accaccatag ccgaaacagg gctagttccc atgatattag agttatcgaa
     9601 ggactcgata cgtactgggg tcgggatttg gagcaaacgc cctagatttt caatagctcc
     9661 ttgagtcttt tcgacagatt tttctagcag attgaacttc tgctctagac taacacgagc
     9721 attttttatg gctagattga ccagttgttt tttctctcca cgttgaggct taagaatctt
     9781 ggaatccacc aaagccttga cagcttcttc gtcaatatcc tgcggaatca gtacctcatt
     9841 gggaactaga tgagattttt cttgatagaa ttgtcctaca taggtcaaaa aatcctcatc
     9901 tggatcattg aagtagggga agagattgac atcgcgctcg atgagcttac cctgacggac
     9961 aaagaaaacc tgcacacaca tccagccctt atccacatag tagccaaaga catcgcgatt
    10021 ttgcaaatct ttcgccatga cccgttgctt ggttcgaagc gttccaatag cctgaatcag
    10081 gtcacggtat tccgccgcac gttcaaactc catactttgt gctgctactg ccattttact
    10141 cttgagatca tcgatgattt tgtcatcctg acctttcaga aaatcagaca cctcctgggc
    10201 catagacttg aaataagcct catccttctt acagatggtg tgggccatac actggccgat
    10261 atggtaataa aaacagacct tagagggcgg gttggtacac ttacgaaaag ggaatatccg
    10321 atccagcaac cgcttgattt cattggctgc ccccacatcg ggatagggtc caaaataaag
    10381 acctccgtcc tttttgacct gacgagtgat aatcaagcgt ggatagcgct cattggtgat
    10441 tttgatgaaa ggataggact tgtcatcctt gagcatgata ttgtacttgg gcttgttttc
    10501 cttgatcagg ttgatttcta ggagaagtgc ctcaatatta gactccgtaa caataaattc
    10561 aaaatccaca atttcagaca ccagagcctc tgtcttggta tcatgacttc cacgaaaata
    10621 ggaccgtact cgattacgca gatttttagc ctttcctaca tagataatgg tgccattttt
    10681 atccttatga atgtagcaac cagggctggt cggcaagagc tctagttttg atttaatcaa
    10741 gttgttcata gttccattat agcaaaaaaa gtagggaaag atagtaccct actcactagt
    10801 ctcatatatt tttcggaggt ttgcaacatc ctcttcctgg ataccataaa aggaacctgc
    10861 tggttgcatg acagacttct catctgtatg gtccaagcca atcgcatgac ctaactcatg
    10921 ttctgccgta tggacaaggc gttcatagga gtagccatag tatggattgg acaaataata
    10981 atgattcaac cgcaccgtta cggacaagaa ttgccctgtt aagagattag tttgactttc
    11041 cgcctctcct gccacaggag tgcctccgtc gttcatctcc gtagccgtaa tatccgcctt
    11101 actagactca gtcacgagtt caaagttaaa agcaccagtt tgattccagt tctggattgc
    11161 ttctaaataa gcctcttgaa agcgtgaatc catctgcgga tccaggtaaa tacgagcaga
    11221 ggcctgtgcc caccttcctt cagaatgctg gtgactatct gtctggctta acttaggcag
    11281 ttgccctgtt ttctcccatt ggtcaatact ttgttgacca atttttactg accgttctgc
    11341 ttgctttagc gctccttgaa aatctccgtt cagataccag agaagtccga aagcaagcac
    11401 acataagagc acaactatcc aaaccagacg ccaaaacaaa cgccacacaa aagaaaagaa
    11461 agcccctatc aaacgaaaaa gccagcgcat gtctctccac ctttctagca aataagaatt
    11521 attttactaa aagaagctaa aagaaatcta aatactgact ttttcatctt aacgtcctac
    11581 tatttttttg agtaactgga tagctttgta ttttaaaggc gatggatggt aacggaaagt
    11641 acctgctttg cgtacaatat agccattaaa attctgttta aaacgcaaaa caccatcact
    11701 tccatcaaaa atcccttgaa tgcctaggaa gttgtattta ggtattccac gttttatgct
    11761 ttccaacata acatattttt gaagcagtgc aggggcatag aacttattaa actcagtgta
    11821 ggaaccacta aagagataag tcgtttcctg aggcatataa acaaataaac tcccagctaa
    11881 aacaatatct tcttctccat atttttcaat caagtctcgc gcttctgctt ttcgaacttc
    11941 aaacgtttca aattgactag aatattctct cagttgattt tgttttttct cagaatgagg
    12001 atttttactc aaatcaagtc gcaacttgtc caagttttct tctagtttac tttgttcacc
    12061 ttgcaatttg ctcatatagt ccgaaaaatt taagcttgct atgagaaact ccgcttgttc
    12121 tccaaaagta tcataaaaat gctcataata ttctaaactt ttatcactat attctctacg
    12181 ttcagaggtt tcttttgtta tattcttaaa aatcgatagt tcttcacgtt ttaacttttt
    12241 caaccgaatg ccaaaggttt cagccttttt caccaagggt ttaccctttt tgctaaaact
    12301 tttaagcaaa ctcttttcag ttaattcagt taaatcttta tagtataacc aatctggttc
    12361 tccacctggg taacctgttg ttaagccatc aaattgataa cctaaatcag tcaaatcttg
    12421 aataatactt tttttctcag catctattgg attaccttgg ctatcaaaag tttgataagt
    12481 ttcatagggt tttacaagca actctaatac accattttgc ttggcatatt cttttaactc
    12541 tgcataaaaa actggaagag catcttgttg ggtataaatc ggccccgaat tgagttccat
    12601 atgcagacca cccagcatgg gcaggctata aaccagagct gcaacttgaa tttctccttc
    12661 ttgtttcaaa gcaagataaa caattcgagc ccctcttttt tctagcaaat cccccatctg
    12721 gacagattgc ataaaggaac gagaagaaac ctgatcagaa taagtctgaa actcttcttt
    12781 cgtgagtgtt gttagtgcca tatacttact ttctatgttt ttttcttaat gttttacgga
    12841 aatcaagagc aagtcttaac agaggataga gaggatgagt gggcattgta aattcaccca
    12901 agtattcttc aatcgttgga ttaaattttt ccttaaaatg ataaagtcca ccattgagag
    12961 agttttcaac accacctaaa ttttgccaga tcatacctcg ttcaaaggca tagcgagccg
    13021 tttcatacca agttaaaatt ggtgcattgt aacgtttaaa atcatcatcc ataccagcat
    13081 atatattgac agaggtagta ccaaattcca aactcaaagt agccgctaaa ggaactctcg
    13141 cttgacctac atctatatat tcctgcaaga aggtcaattc ctctaacaaa cgttcttttt
    13201 ccttcttctg cgcttctact tttgaagttc gagtcgactc agtaaaggtc tcttccaagg
    13261 ctctattttt cgctaactgt tcttctaact cttgcgaacg tttagaaaca tccaaggtgg
    13321 ccaaggtgat ataggccttg tccttaaaat tatctaacaa ttttttataa taggcttcat
    13381 tcctcaaatg aatctctttt cgcttctcag tttttttcat caactccgaa aatgaatcta
    13441 atagttccag tccaccatat tgaatctcaa gccctttgtt tcgtgctgtt cgaatagcct
    13501 gttttgttga cttggaaagt ttatcttctt caaaattttc cttgtatatt ttcgcctgaa
    13561 tacgaggttg aatggtgtct cccatttcct ccgtttttcc tgaccacctt actcccattt
    13621 gttgcaaact atcaataata gccagatttt caggaaattc tgtcttttcc tgattgatta
    13681 aactttgaga taggcaaata cttgggtcaa aagtcacaaa aaccgctctc ttactgcgag
    13741 cataggactt aatagactga atggcaaaat tcaagagttc tttatcccca taatccaata
    13801 taggtcctct tgggatgtaa aacattttat agcctagcgg aagagttcta atcaaaatac
    13861 tagctgtcgc cagtaatttt tcttccctgt aaacaccaaa cttctcatgt tgccaattag
    13921 acttaacttc ctcccaagca ctactttgta atacattggc taattcatgt tctttgacaa
    13981 actgatcata ttctaatgtg ggaatgccaa tttgataacg gtacatttcc tactctcttt
    14041 cctccagtat tttattacta ttctactact ttcgtttgaa aagtctagtg aaaacatatt
    14101 ttattttagt tctactcccc ctatgctgaa gttcaaacta agtcagtctc tcttctaatt
    14161 tgaaatcaat tggtagggtt gttaaaaaaa cttccaattg tttttcccag tagtaccact
    14221 cgtgagttcc agcgctatgg ctataggtca catcaaaacc tagttttttg agatttttca
    14281 ctgcgagatt attggcttcg tacaagaaat cctgttcgcc acaccacgcc caaagtttgg
    14341 tctttttatc cgatttttta gccagacttt caagagaata gggactagtt gtccagtctc
    14401 taatctctcc aaaaacacct ctccagtagg ctggacttcc cagattttga ctttcaggag
    14461 aaaagttttg aaagctgagg gcacctgaaa aactagctgc atgagaaaaa cgatttgtcg
    14521 taagagccag tttgaagcag ccgtagcctc ccatagaaag accagcgata aaggtctttt
    14581 cacgcttgct cgtcatatta gggaagaagc gtttcagaac ctgtggcaat tcctctgcta
    14641 gagccgtgta gtagtcaaaa ccatactggg tatcggtgta ccaaccattg ctggtattgg
    14701 gcataacaac gatgagatta gttcctcgaa gcaagcgttc tacattggtc cgcttaagcc
    14761 aactattatg atttccagac atcccgtgca aaaggtacaa gacgggaata tcttcacact
    14821 ctggttcttc cactcgattg gcatcagggt agaggacatt caccccccac tccatatcca
    14881 atacttgtga gtaatactcg attttcatca ctgccatgat tttctccttc tatttttcta
    14941 taagaaaaag ccgaaactcg actttctctc tgtttatttc aacgattatt ttgcttccat
    15001 gactactggt aaaatggctg ggcgacgctt ggtttgatca aagaggtact tggtcagatt
    15061 gtcacgaacc ttacctttga gatctgccca gtcaaagtca tctccttgaa gatactcttc
    15121 taccgtttgg ttaatcaatt ctgaactttc acggagaata tcgcgactct tcttgagata
    15181 aacaaatcca cgcgtgtgaa cacgagccct agccacaatt ttcttctcac gacggttgac
    15241 tgtaatagcc acgatgaaaa ttccatcctc cgacaagacc ttacggtcac gaagaacaac
    15301 atttccaaca tcaccaatgg cattcccatc aatcaagata tctcctgctg aaaccgatcc
    15361 agctggaaca aagtctccat tctcgtaagc catggtcgtc ccctttttag gaatgaagat
    15421 gcgttctggc aacatcccaa ctgccatggc agccttagcg tgagcatcca actcacgata
    15481 ctccccttgg acagggaaga ggtacttagg ttgcaaaaga ttgatcatca gctgcaaatc
    15541 acgcacattt ccgtgccctg atacatgtaa actttgggta atcaatttga caaccccacc
    15601 tgcctgataa atcatatttt ccacacgcgc aacaaaggct tctttagcaa tagacggagc
    15661 cgtagcaata tagactaggt ccccatcctt gatttctaca taacgatggc gaccaatcga
    15721 catcttacga agtccattga taggctcacc catacgacct gtctcaagaa taatcaactc
    15781 atggtcttca aagcgagaca tatctttagg cttaatcaaa agaatttcgt tggctaaaga
    15841 caacttctta agacgaatcg ctgtgcggac gatattttca atatcaaatc ctgtcaagac
    15901 gatacgtcga cctgttttat ccgcagcgtc aaaaatctgc tgaatacgag aaagattact
    15961 ggaaacagct gcaacgatga tacgaccttc ccagtcagca atagtttggg taatttcatc
    16021 cctaacttca ctttcactag ccacctgaat attgctgtct gcattggccg aatcactgag
    16081 gagagccagg acgccgtcac gaccaatctc tgccaaacga gcaaaatcag ttgcataaga
    16141 ttcactagcc gtttggtcaa atttgaagtc acctgtataa acgatgcttc cttccgatgt
    16201 cttcaagaca attcccagac tctctggaac ggagtaagtc gtagggaaga aggaaaccac
    16261 tgtcccacca aaatcaatct ccgtattctc atcaatgaca tggaaatcat taaatttctt
    16321 aacggcatca tttcctttga caaagagctt tgccaactca atggtcaact cagacccaaa
    16381 tacaggaact ttagcctctg ccaagagata cggtagagca ccaatggcat ccgcatgccc
    16441 gtgggtcaag aaaaccccag caatacggtc gctattttca aaaaggtaat ccatgtttgg
    16501 aatcaccaca tcgaccccta attgttcatt ttcaggatat tttaacccta cattcaaaac
    16561 aaaaatggac tctccaattt cagcaatgta catatttttt ccattctcac gcacaccacc
    16621 aagtgttgtt aaactgatat tactcatttt tcctccaaaa tcttagttta tcttgattat
    16681 agggcaattc taccctctta ttctaaaagt tctgatactt tcagccgaat cttttcctac
    16741 cattatacca tttttttgat gattttcaaa atgaaggtct gttttcaaaa aagagttgat
    16801 ttcccaactc tttcctaact tctactcttt ttccatcaaa aaatcataaa tttcttgcac
    16861 cgtacccagc gccatcattt cttggtcttt aacggtttgt ttgagatttt ggtaaatttg
    16921 actttctcct atctcccgct ttggcgcctc tttattgact gtcatcacac catcttttat
    16981 cgtcacaaag cctagttctt caaatacttg aatcatcttg accagcaaga tttgttgaat
    17041 attaagatat gcagccaaat ctttcagctt gtagcgaata tcaaactctg ggaactggta
    17101 aatagtcttg tacaatttgg caaactgatc tctagtccca taacctgtca gataataagc
    17161 cttgtcaata tcatttttga aatagacagc agagaaatgc tgttcctgaa aaatggtctt
    17221 cagctgagta atatcctctg gaatgttttt tacgacaaca gcttcactag ccgcaagatt
    17281 tggcagttct ccaggaaaat ccaagactgg aacaccttct ggcaagactg cattttttcc
    17341 acgaatgtta aaaagttgaa caccttccac tcgcgcatcc accatcatca actggagggc
    17401 agtttggcca ttccattggt tgacagacaa tttaaccgct aactctagat tcttggtttg
    17461 agaaaactct gtcgcccatc tgccttgacc aaaggctacc acttcaaaac tcgcctcacc
    17521 cttggaaatt ttcagcttta gatgggcatt acctgccccc atagtacgag cactttcgac
    17581 ctgaaaattc ttgatataaa aaataggttt ctgattatcc attccaaaag gagctaaacg
    17641 ttcaaaactt ttgaccgttt ccaagctaag tgcctccaaa tccaactctt catctaggtt
    17701 taacttattc ttgccaccag catctgcacc tttttcacga acataatctt ccaaaacctg
    17761 agataaatct gagagttgct caacttccag cgtcataccc gctgcacctg catgacctcc
    17821 aaaggcgatg aagaggtctc gatggggatc cagagcttca aaaatatcga ccgcttccac
    17881 actacgagca ctgcccttgg cacgaccgtc ttctatatta agaacaatga ctgtctgtcc
    17941 caattcttcc aataaacgac cagccacgat tcctagaacc ccaggattcc agccttcctt
    18001 ggccaagacc tgaaccttct tctcaggatc caccatggtc ttggcttctt catagataga
    18061 ctgaacgatt tccttgcgct cttcgttttt ctggtgaatc ataagggcaa tctcatgcgc
    18121 ttcctcatca tcaaatccag tcaacaaatc aatggcagga ttgggatcat ccaagcgacc
    18181 caaggcattc aaacgaggag caatctggaa accaaccgtt tcttctgtta cttcgttggc
    18241 agcaatccca gccatgtcca gcatttcttg cagaccaatg cgctgggtat gacccaacat
    18301 ttccagacca tattgaacta agatacgatt ttcatccgtc agactcacca tatctgcaat
    18361 agttccaata gcgaccaaat caagcaattc cacttgcact tcttctaaca gggcacaagc
    18421 caacttgaaa gcaactccac aaccagccaa atatttaaaa ggataatccg catctggatg
    18481 ttcaggatgg acaatagcat aagcatctgg cagggtttca ggcatggaat gatggtctgt
    18541 cacaatgaca tctactccca tagactgagc caatgcaata gcctcatgac cagcaacccc
    18601 attgtccacc gtcacaatca aggaaatccc ttcttgctcg ataaagtatt tataaacact
    18661 agcattaggg ccatagccat cggtaaaacg atttggcagg taaactcggc actcagcacc
    18721 aagttgttcc aaactttcct tcacaataga agccgaagtc atgccatccg catcatagtc
    18781 tccataaaca agaatatttt ccccttcttc aatagcctga cgaatccgct ccactgcctt
    18841 gtccatatca tggagcagat aagcatcatg taagtcctcc aaggaaggtt ctaaaaactt
    18901 cttcagactt tcttggttct gaatccctct ctcaaacaat aaccgagcca cctcaggacc
    18961 cagtccagcc ttcttggcta tctttgtaaa atccgcatct tctacctgca gggcaaactg
    19021 ccattcataa gtaggtgtta tcaaaaagac atccactctt tctaagaatt ctattgcttc
    19081 attataacat gatttagacg ttttctctat ccagattgct tttttataac attttatagt
    19141 atattgaaac tagaatagta cacctctact tctaaaatat tgttagaaat cgatttgact
    19201 gtcccgatcg atttttcctg ttcttatttc actatagtga attttttcag atatgatttg
    19261 acaagaacat tcttttattg tagaaccgtg ttataaaata taacacaata gagatctctc
    19321 atggctttag tagaatttaa aaacgtcgaa aaatattacg gagactacca cgcattccgc
    19381 aacatcaatc tccgttttga aaaaggacaa gttgttgtcc tgcttggacc ttctggctct
    19441 gggaagtcca ctcttatccg tacgatcaat ggtttagaga ctgttgacaa aggaagtctc
    19501 ctagtcaatg ggcaccaagt tgctggtgcc agccagaaag atttggtacc tcttcgcaag
    19561 gaagtcggca tggtttttca acattttaac ctttatccac acaaagctgt gttagaaaac
    19621 gtaacgcttg cacccattga agttctagga attgataaaa aagaagctga aaaaaccgcc
    19681 caaaaatatc tggaatttgt aaatatgtgg gacaagaaag attcctatcc cgccatgcta
    19741 tctggtggac aaaaacagcg gatcgccatc gctcgtggtc ttgctatgca tccggaactc
    19801 ctcctctttg atgaaccaac atctgctctt gatcctgaga ctatcggaga tgttctagca
    19861 gttatgcaga aactggcgca tgatgggatg aacatgatca tcgttaccca cgaaatgggc
    19921 tttgctcgag aggttgcgga ccgcattatc tttatggccg acggagaagt tttagtagat
    19981 acgacagatg tcgataactt ttttgacaat ccaagcgaac ctcgtgccca acaattcctc
    20041 agcaaaatta tcaaccacga aagtgacaaa gtcaaataag gaggcgccta tgaaaaaaaa
    20101 ttctttttat cagcattatt gattagcctt ttcggccttg ctgctgccaa accagtccag
    20161 gctgatacaa gtatcgcaga cattcaaaaa agaggcgaac tggttgtcgg tgtcaaacaa
    20221 gacgttccca attttggtta caaagatccc aagaccggta cttattctgg tatcgaaacc
    20281 gacttggcca agatggtagc tgatgaactc aaggtcaaga ttcgctatgt gccggttaca
    20341 gcacaaaccc gcggccccct tctagacaat gaacaggtcg atatggatat cgcgaccttt
    20401 accatcacgg acgaacgcaa aaaactctac aactttacca gtccctacta cacagacgct
    20461 tctggatttt tggtcaataa atctgccaaa atcaaaaaga ttgaggacct aaacggcaaa
    20521 accatcggag tcgcccaagg ttctatcacc caacgcctga ttactgaact gggtaaaaag
    20581 aaaggtctga agtttaaatt cgtcgaactt ggttcctacc cagaattgat tacttccctg
    20641 cacgctcatc gtatcgatac cttttccgtt gaccgctcta ttctatctgg ctacactagt
    20701 aaacggacag cactactaga tgatagtttc aagccatctg actacggtat tgttaccaag
    20761 aaatcaaata cagagctcaa cgactatctt gataacttgg ttactaaatg gagcaaggat
    20821 ggtagtttgc agaaacttta tgaccgttac aagctcaaac catctagcca tactgcagat
    20881 taaggaggac accccatgac agatttatca tcttggacag cctattttca ggattttgga
    20941 caatttttca atggtttcct cttcaccctt gccctagcgg ttggttcctt tatcctcgct
    21001 atggttttgg gcatcttctt tggtgccatg tcaaccagta aacgtccaat tttacgcatt
    21061 ttagctcgca tctttgttga attttaccaa aatactcccc tcttggtgca gtttgttatt
    21121 gttttttatg gcctacctct tatcagtgac catatcatca tgattccaat ttattggaca
    21181 gccgttctct gcgtgggact ctatcacggc gcttatatcg ctgaggttat tcgttcaggg
    21241 attcagtcta ttcctagtgg tcagatggag gccgccttgt cgcaaggttt tacctatatc
    21301 agtgccatgc gcttgattat cttgcctcag gccttccgca tcattctccc tccattgacc
    21361 aaccaaattg ttaacctcat caagaacacc tctactgtag ctatcatctc tggagtagac
    21421 ttgatgtttg tgactaagtc ttggtcggct ctcaacggaa actatatacc agccttttta
    21481 ggtgctgctc ttctctactt tgctctatgc ttccctgttg cccagtttgg tcgcaagatg
    21541 gagcaagcca acaaaaaagc ctattcactt taggaggtta ctatggaatc tattttagaa
    21601 gttttaaccc cagataacct agtctttatc tttaaaggat ttggcttgac cctctatatt
    21661 tctctgattg ccatcatcct ctctactatc atcggtacgg tgctagctgt cacgagaaat
    21721 ggcaaaaatc ctgtcttacg cattatttcc agtatttata tcgagtttgt gcgcaacgtt
    21781 cccaaccttc tctggatttt tactatcttt ttggtgttca aaatgaaatc cacaccagca
    21841 ggtattacag cctttactct ctttacatca gcagccttgg ctgagattat tcgaggcggt
    21901 ctcaatgccg tagacaaggg acagtacgaa gcaggaatgt cacaaggctt cacctcagcc
    21961 caaatcctct actacatcat tctcccacaa gccatccgca aaatgctacc agccatcatt
    22021 tctcagtttg ttaccgtgat taaggatacc agtctcctct actctgttat cgtcctacaa
    22081 gaactctttg gagccagcca aattctcatg ggccgttatt tcgaaccaga gcaggtcttc
    22141 agtctttaca tcctgattgc cctcatctac ttcagcttta acctagcaat ttctagcctg
    22201 tctcatatgc tagccaaacg ttggcaacaa gctgcagaat aacactcttc gaaaatctct
    22261 tcaaaccacg tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ttgcggctag
    22321 cttcctagtt tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg
    22381 agagtttttt gatgagagta gatatttata gacctcaatt tgacaccata cattttctat
    22441 gataaaatgt aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta
    22501 tgaaatcagt caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt
    22561 ggatgaacac ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga
    22621 cttttatcct agccactcgt ctcccactac tagaaagctg gtttcacagt ttggagaagg
    22681 tctacaccgt ccacaaattc acagcctttc tctcaatcat cctactaatc tttcataact
    22741 ttagcatggg cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct
    22801 atatctttgc cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt
    22861 ggcgatggat tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca
    22921 tgataatggg caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg
    22981 cccttttagg cttactagct ggtttttata tcatttttct atatcaaaag atttccttcc
    23041 cctatctagg gaaaattacc catctcaaac gcttaaatca cgatactaga gaaattcaaa
    23101 tccatcttag cagacctttc aactatcaat caggacaatt tgcctttcta aagattttcc
    23161 aagaaggctt tgaaagtgct ccgcatccct tttctatctc aggaggtcat ggtcaaactc
    23221 tttactttac tgttaaaact tcaggcgacc ataccaagaa tatctatgat aatcttcaag
    23281 ccggcagcaa agtaacccta gacagagctt acggacacat gatcatagaa gaaggacgag
    23341 aaaatcaggt ttggattgct ggaggtattg ggatcacccc cttcatctct tacatccgtg
    23401 aacatcctat tttagataaa caggttcact tctactatag cttccgtgga gatgaaaatg
    23461 cagtctacct agatttactc cgtaactatg ctcagaaaaa tcctaatttt gaactccatc
    23521 taatcgacag tacgaaagac ggctatctta attttgaaca aaaagaagtg cccgaacatg
    23581 caaccgtcta tatgtgtggt cctatttcta tgatgaaggc acttgccaaa cagattaaga
    23641 aacaaaatcc aaaaacagag cttatttacg aaggattcaa gttcaaataa gtaatcatac
    23701 tctcctggga tttcccagga gagtttttta tagccaaatg aaataagaca ggaacgatgg
    23761 aattttggca ttcaaatcaa ttgataagac tattttatag tggagtgaaa taggatacga
    23821 acaaattgat taggaaaatc aaatgaattt atagaaatct tttagcagtt atgttatcct
    23881 attctagttt caaaacgcta tagaagcagc attgtgctag tctagattca gtttactata
    23941 ctaaaacgag tagcttgaaa tcaaaaaacc caccctcaca ggcaggtttt atctgtatta
    24001 ttcagctaga ttatgcttta ccttctgaac cgaatacgtc gatacgttct tcaaccgatg
    24061 cttggatagc ttttacaccg tcagccaaga atttacgtgg gtcgaagagt tttttcttgt
    24121 cgtattctgc ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg
    24181 cgatttggca ttctgtgtta acgttaactt tggcaacacc aagtttgata gctgcttgga
    24241 tttgctcatc aggaatacct gatccaccgt gcaatacgat tgggaatcct ggaagagctt
    24301 ctgtcaattt ttgcaagtgg tcaaggtcaa gaccttccca gtttactggg taaggaccgt
    24361 ggatgttacc gataccagct gccaagaagt cgataccagt ttcaaccatt gctttagcgt
    24421 cttcgattgg agccaattca cctttaccga tgattccatc ttcttcacca ccgatagtac
    24481 caacttcagc ttctactgag atacctttag cgtgtgcttt ttcaacaact tctttagcca
    24541 atttaaggtt ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa
    24601 cttcgataca ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata gctactggta
    24661 cagtgatacc cattgattca acaaggttag cgatcaagtt gcgagcaact ttgtaaccac
    24721 ccatgtattt agcagcaccc attgaagttt ggatcaaaac tggagctttt ttagcttctg
    24781 ctgcgcgcaa gatagcttga gtccactcaa ggttgtttgt gttaaatcca ccaactgcat
    24841 aaccgttgtc acgggctgct tggacaaatt tttctgctga aacgattgcc attttatcag
    24901 gcctcctgta tatttttatg ggtcatccca tttacattgt tcattttatc actttttgcc
    24961 aaaaaaatct agtttttccc gcagtttcga ttgattttct tctaactcca tctatgtaaa
    25021 ccctttctct ccctagtctt ggacgacttt tggaaaatct ataaagaagg ttaaactatt
    25081 ctcctccatc tcgaaacgat aagctaattt ttcatgttct aatagactct taaccacaaa
    25141 gagccccata ccagacccct tgaccttgcg actggcattg tcagaaaaag actgggctag
    25201 tttttcttgt tcctctgagc tacagctatt ttcgataaaa agttctcctt ctctttctcc
    25261 aattcgaact aagccacctg gaacagagtg cttaatggca ttgctgatga gattagaaag
    25321 aatcaacttc ataactgatg ggtttagata agcctgctga tgggtcaaac tattgtctat
    25381 ctggagctct ctttccttgg ctagcaaggc ataatctttg accagatttt gcgtcatctg
    25441 gaggaggtca attgtttccc tatcatctcg caattcctgc acagaagaga gggaaagtat
    25501 ctgcagaaca tggtgattga gttcatccac aatccccaag gcaactccca gatactggtc
    25561 tctatcctta taacgaccga tattctctct catattttcg attaggattt tcaaactagc
    25621 cagcggtgtt ttcaattcat gagaagctcc tcgtaggaat tcgaccttca tcttctccag
    25681 ctggagaatg gcttcattct tttcatgcaa gtccgcaata acagtcaaga gatgctggta
    25741 gaggctattg atttgttcct tgagattacc tatctcatcc ttagaatcca cgcgcaatcg
    25801 cacttgggaa tccaggtcca tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc
    25861 aacaatagtc cgagcgtaga tgtaggccac caaaagggaa atcagaaagg aggccagcaa
    25921 ggtataggga agaaactgga gactgatttg ctccgcttcc ttttgtaaat ccatggaagc
    25981 tagaaactgg agaatcatag taccaccgtc ttgcgttttc acctcgcgct cctcaataaa
    26041 gagagaggtt gtctggcggt ctgtgtccag aggaagactg tccttgactt ctaacttgtc
    26101 ctcggtcatc tcacctttga cggtcccctt gatatcacta gtctgggaat acaagtctaa
    26161 cacttgctcg atactctgcc tatctttccc ttctagggac tgggcaatgg ctgttgcctt
    26221 ttgaccaatg gtttcctgac gatgactcag ataagtcgaa ggaaaaagaa aataaatagc
    26281 taaatgaagg cagataacca gaacactaaa tatcgagaag gtatagataa atatctttgc
    26341 aaataaacct gttcgtttca ttttcgctcc aatttataac caacattgcg cacagtgagg
    26401 atacaatcca agtctagctt tttccgcaat tccttgatat aaacatcaat aacacggtca
    26461 aagggaacct catctgtcgc tttccagacg gcatcgataa tctgagatcg agtcaaggcc
    26521 cggccttcat ttttcactag atagtccaga atttccaact ctttggcatt gataggcact
    26581 tcttgacctg cgaggcttgc actgtagctt tcaaagtcca ccttggtatc cttgtaagaa
    26641 aagattcgtc ctgtatcgta gtagcgcttg aaaatcgcgt ccaccctcac ttttaaaagg
    26701 gagagggaga aaggtttttc cagatagcca tctgccaaag aggcaaaggc actcatcttg
    26761 tattcctcat cttgaaaagc tgtcaacatc aagacaggaa cctgactggt tttacgaatc
    26821 tcagctagga cttctaagcc gttgagcttg ggcatctgga tatccagtaa aaccagggcc
    26881 acctcatagc tagaaaattg ctccagagct tcctgaccgt ccgctgcctc aatagtttca
    26941 tagccacaat ccgtcaaata atcactgacc ccctcacgga tcatctcttc atcttctaca
    27001 attaaaattt tcatacttta actgctctct attttttatt tttcttagaa taaataccta
    27061 ccctattttc tattatagtc tcttgctggc cttttgtctg caagcaactg accactagat
    27121 aaaacgttgt gaaattcctt tctcataaat tccataactt tagtatatta tatttaagca
    27181 ctaaagtaca aagaaagcaa ctgaaagcaa tgattttcac cactgctttc ggatttattt
    27241 tgaattgtta aatagccatt cctatccact attcttgaat agaaacacaa gatgcaatct
    27301 ttattctaga ctcatttttt caaatttatt caccatccag caagagctct tttggttgtt
    27361 ttctaaggag attgcttgaa gcaagcgcca taacgagaac cactagaacc aaggcaagga
    27421 caaaaatgat gataaagtct gatgtctgaa tggaaatgtc taggctcgac aaggtcttgc
    27481 taaagccatc tacttctgca ccaccaccaa ggttagaggc ttgagccgcc ttactagcct
    27541 gtttggcaac acctgaagtc acattggcaa ggacagtgtt tccaattgca cgggcagtgt
    27601 aattagctag gaagtaagca gaaactagag cagggatagc aatcaagata gattcggtga
    27661 tgaattgacc caagatactt gcctgcttga ggccgataga gaggagaatt cccacttcct
    27721 tgcgacgggc gttgatccaa aggctgagca agagggcaag gaggagaact gagaagctca
    27781 agctacccca gaagaggagg ttggccatct tgtacatacc agagatagat tgctcaagag
    27841 ctgggtagtt agaggagctc ttgacgagtg tgtagctctt ccagttgata ccactgatgc
    27901 cattcaactc tttcataaca tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt
    27961 ccccataaat ggctgtgtct tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg
    28021 taatagctgt gttttcgtaa agttcttgtg agtaggttac tgctgactta ttatgaccat
    28081 caaagagtcc cttgattgtc acttcaactg tttccttggc tcctttttca ttatctgcat
    28141 cgtagatatt agagtccagt ttaaccttgt cccctacttt ccagccgtgt ttggctgcca
    28201 agtccttgtg caagaggatt ttatccttgt cgtcgttggt taagtgctct ccttcgacta
    28261 gtttataaga accagagaca aacttgtctt ctttagagga gtcattgaca cctgtaatca
    28321 tcaagctact tccaaaacgc ttggcacgat cagcagtgag attcttcttg gtttctggcg
    28381 tttcaatcag gtcatatcca gtcaaatctc cgatagcgtt gatacgtttg acataagact
    28441 caatggcctt gttttcggtg atttttttga tgtcttcacc cttgatattc ccagcaccac
    28501 gaggcgttcc ttggttgacg cgacgattga tttgcatgga gaagctattg gtgatatttt
    28561 taaaggtctc ctgagaagcc ttggcagtag ctcccttgat tgacaagccg accaaactca
    28621 agctcgccat gaggagaata atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa
    28681 cataggcaaa tgcgttgtgt aacatagatt ccctttctag attttgtttt aatcattcta
    28741 ttaaaataag ctcaaattat ttactagtat tgcgcgtttc agtcagtttc ttatccttta
    28801 attcaagtgt aatatctgac gcttgtgcca cttctttact gtgagttacg acaatcacac
    28861 atttacctgt tttctgggca agtgatttga gtagttcgac aatatctcca gcagttttag
    28921 gatccagatt tcctgttggc tcatcagcta gaataactgg agcttctgag accaaactgc
    28981 gagcaatggc aacacgttgc tgttgaccac ctgataactg gagaacattc cgcttgatct
    29041 ggctttcatc caaaccaagc tcaagaagtg tattcttgct tgcctttttg ttgaccaatc
    29101 ggatattttc cagcggagaa agataatcta tcaagttata attttgaaag accagggaaa
    29161 tatggtgcat gcgatggtaa gaatagccct tcttacgaat atcctctcct tgaaaaagga
    29221 tagaaccttc aacaggacta tctagaccag caagtaggga caagagtgtg gattttcctg
    29281 ctcctgactc cccaataata ctgtaaaatt ttccgggttc aaaattataa ttgatctgat
    29341 ataggactgc ttcagcagta ttcttataac ggtaggtaac atcttgtaat tgtaataaag
    29401 tcatgatttc tccttcttaa ctaatagatg ataaaatttc tttcggtgat tttctaaata
    29461 agaataggaa acaaagggct acagataagc aactaagcag aactagaaaa acataggatt
    29521 ctgcaaaaga taagatgcta gttgataaac tgcttgcttt ggctagtgta tcttgtaagc
    29581 ttgcctgatc tccacttgct agtagagttt ggagtaggta agttgtgatt gcgtttcctg
    29641 caacaaatgc tggaagcaaa gctccaagag ataccaaaac tacctctaaa cagaattgta
    29701 ggaagatcga gctcttgcct tttccaagtg caagtaaaat ccccacttca tagacccgtt
    29761 ctctcaacca gagagacaaa accagaatta aggctccagc tcctgctatc aacatcccat
    29821 aaaggaagat ggtcaggaag gtttggaaag ttgcaactga gtctttgatt tgttcaaaag
    29881 ccttgttttc cttttcgact tggtagcctt gattttccaa ggccaagttt tctacctgct
    29941 tcatgagtcc gtccatttcc ttaggatttt ctacatagaa gcgtgctgca ctgacttgag
    30001 cttcactatt gcccaaaagg gtttggctac tttcatagtc tgtaaagact tgattttcac
    30061 tgaagtcaga agacaagcct gtgaatttct cttgtttttt accagaaaag atgccgataa
    30121 tctcaaactc tactgtttgt ccttttccag attcagactg accagcatcc aagccaatct
    30181 tgtcatgaag cgaaagaccg ttcttcttag ccaattcttc gtggataagg attttcttgg
    30241 aatccccttt ttgaaggtgt cgcccttctt ttagattgaa agccgaactg gtaaaggtta
    30301 catccttgga tgaatcctca agagccgtta agctaaccaa gttattgtct gcagctgata
    30361 aatcatcacg ctccacgctc tgctcgccag tcactgcttc cttgtctttt agttttgcga
    30421 ccgtctcaag ttcaggagag acattttcca gccccttaat cttgcttaca gatgctaggt
    30481 ctgacaactt gaatgtctga ccattctcta tcttcttaat agaaaaagat gtattgagtg
    30541 atttataaag attgctttct actgttttgt tggacttcat cagagtcaaa caggctgaaa
    30601 ttccggccaa taagaccaat aaaatcagaa ataaaataaa acttctcagt cgctttctgc
    30661 tgacataagc ccaagatctt tggattggat tcatttgtca cctccatatt tgtaagacta
    30721 ttataaaacc caaatatgaa atatttatga aatacgaaaa aaaaatatcg agtaggggat
    30781 aatctctagc ccctctcaca ccaccatacg tgccgttcgg catacggcgg ttcaactaac
    30841 ttttaacgca tgtcgttcaa ggtaataatc caaacacgaa accagtccac gtttttcaag
    30901 gactggtttt gatatagcac gtttaagtac cgacttctga gctactatag tagattgaaa
    30961 ctagaatagt acacctctac ttctaaaata ttgttagaaa tcgatttgac tgtcctgaac
    31021 aattcgtcct attcttattt cattttacta taattgatag tggtcgcccc agccagatac
    31081 cttatctgct atccatttag gaacccctaa cttaagcaat ccccataatc gtctcgattt
    31141 cttcttccat tgcttccaga taatcactcg taggcgagta cgcaagcgct catctatgct
    31201 agtgactata cttttcatat ttataattca ttcctttcgt ttcactcaag gcacaacaca
    31261 gaatgaaaaa gtgttgtgat ctttattttg ttttataata atagtgagaa aacctatcac
    31321 tactacaaat cacggggagg tgaataagtg agtggtacag ccactacctc gcatattttg
    31381 tcacatcatt taacggtaca taataagttg taccatctga ataagttgct acaatatcat
    31441 ttgcatgctc tccttcacct ttagcaaagg ttggagctcc tgctggatga tttttatttg
    31501 cctctttcaa tttttcaata atggcatttt ttctgtatct tttatattat caggattttt
    31561 cactaagatt ttgtctggat atgtcggttt agcagaaaca atttttactg ttacttcttt
    31621 tttattcgaa gcacttgtcc agtttccagc attatcttta gcatttaatt ttacagtaat
    31681 tcctgaacta ggaacttcag tagcaggttg attatcaaca ttattcaact ttaatttcaa
    31741 aagagctgtt gcatcagacg ttttatcaat cgttatatat aatgatgaat tgttattata
    31801 aacagttcct tcatatttag ctgtttgtga gctacttgaa acagaactga aattataccc
    31861 actacctccc tgattatctt caatgcttac gtctaaatga acttccccac tattatttgg
    31921 cttagcaaca actgttatag taaaataaca taaaatttgc ataaatagat tagggaaatc
    31981 aaagcagctt ctaggaatgt tttagcagtc acagtgtact ttcccagcat caagccacta
    32041 taactctgca cataaaaatg gagaagatgg caatcctctt ctccaaatat taacttcttt
    32101 acaaaccaac tatagttgac aaagaaccta aaatcaattg ataacacaag gtcaggtcgg
    32161 tcaactcttt caactgaagc cctgtcaact cttcccattt atcaatcttg tattggagag
    32221 aattgcggtg cagatagagt tgctgggctg ttttagtgag aacagcacta ttttcccaaa
    32281 gagagagaat gatttcctga atctgatctt gatccaaaat catctggtgt agacattcct
    32341 tgattggctt caagtccacg agtctttctc ccatactcca aagatagagc tgagaaaaag
    32401 tatgaacacc ttggtgaccc tgacgccacc atgtcttgaa caaatcccgc tcagctttga
    32461 ttaagtctga tagggcttga tgtcccgtct gagaccaaac ctgacccaac atgatagaaa
    32521 gacgaagtcc aaagtcatac tcaaccgctt caatcgtatc acttaaaata tctcttacag
    32581 aagtgtattt gtcttgttga agcacgaaaa cataatcctg agctccgacc tgtagcactg
    32641 tctgacaatt cggaaaaaga gtccgcatca tatctagcca agaagccaga ttttcctgct
    32701 gaaaataaga aagatggcaa taaaccaact gaatcttttt aaaaacttgc ggtgcctgtc
    32761 ccttgccctc aaccagatag gaataccaag ggtttagcga acgagcctgc tcctgctggg
    32821 tcaaaagggc aaccaactgc ttttcacgct cgctgagccc agcttcctcc agcaaaatcc
    32881 actgctgaga agctaaaggg agcgtgagat agccctcttt ctctactggt tggtctgaaa
    32941 tccgagcctc aggaaaccag tcttgtagtt cttttgccct catgttctag ccctccactt
    33001 tttggatgca ccatgaaacc aaactctcaa gacgttccag attctcagtc atatggagat
    33061 agcccataac cgcttcaaat cccgtggaca tacgataagt cacgacatct gcatttttag
    33121 cctttgtgtg gctattggta ttgcggccac gtttgtagat ttcttcttct ttttccgtta
    33181 ggacctgctc ctccaacatg agagcaatca ggcgagcctg agccttggct gacacgtact
    33241 tagttgcttc ttgatggagt ttattgggtt tggtcatacc tttgaggatg aggtgacggc
    33301 gaatatacat agaatacacc gcatccccct caaaggctag cgcaatcccg ttaatgagat
    33361 tgacatcaat cacgtgtcca cctcactcca tccttggtat caaggagctt aattccttga
    33421 gtaaccaatt ggtcacggat ttggtctgct gtcgcaaagt cacgattggc acgcgcctct
    33481 tggcgttttt gaatcaagtc ttcaatctct gcatccaaaa cttcctcaac aaagacaatt
    33541 ccaaaaattt ctaacatatc tgcaagagct tgcttgacac ttgcatcata gttccctgag
    33601 ttgatccatt tggccatttc aaagacaact gtgataccgt tggcagcatt aaaatcttca
    33661 tccatagctg ctacaaactt atctttaaag ttttgtaact cttgggcatc cacatttcct
    33721 gtaaatggtt gttcgtaagt attcttgaga tacttgagat tggtctcggc atcgcgaact
    33781 gccttttccg tgaagttgat aggcttacgg tagtgctggg tcgcaaagaa gaaacgaagt
    33841 acttgcccat caagagtttt aagggcatcg tgtaccgtaa tgaagttacc caaggactta
    33901 gacattttga cattgtcgat attgacaaag ccattgtgca tccagtagtt agcaaaagcc
    33961 ttgcctgttt tagcttcaga ctgggcaatt tcattggtgt ggtgtggaaa ctctaggtca
    34021 gctccaccac cgtggatatc aatggtatca cctaaaatct ctgtcgacat gactgaacac
    34081 tcaatatgcc aacccggacg tccaggtccc caaggactat cccaagaaat ctcacctggt
    34141 ttggaagatt tccatagagc aaagtctaca ggattttcct tacgagccgt ttcttcatcg
    34201 gtacgacctg aagcacctag ctccaaatct tccaaggttt tattagccaa tttagcatag
    34261 ttgtgggatt tttctacacg gaaatagaca tccccttgac tctcataggc aaagcctttc
    34321 tcgatcaagt cttccacaaa acggatgatg tctgccataa actccactac acgcggatgg
    34381 cgagtcgcag gtttcacgcc caatgccgtc acatcctcac gaaaggcagc gatgtactta
    34441 tccgcaacct cctgaggcgt gataccttct tccctggcac ggttgataat cttatcatcc
    34501 acatctgtaa aattggaaat ataggcaacc ttatacccac ggtactcaaa atagcgacga
    34561 atcgtatcaa aagctaccgt cgaacgggcg tttcctacgt ggatatagtt gtacaccgtt
    34621 ggcccacaaa catacatctt gatcttgccg tcctcaatcg ggacaaattc tcgcaaatca
    34681 cgagacatgg tgtcatagat tttaatcata aatcataatc aggaaagctg aaatccaaga
    34741 acaattagtt tcatcactaa aagttcaagt aaatttcagt ccgaatatct ctacacttcg
    34801 gaatcccttg ctcctttctc attcagataa accacctgag tctgtttgac aaagccaatt
    34861 ttttcataca aacgtttggc acctacattg ctatcttcca ctgcaatctg aaattccttg
    34921 tcattttgct caattagttg gttgacgagg gattttgcta agtagcttcc atagcctttt
    34981 ccacgttcag gttccaatat tgctaaaccg tagaggtaat tcgtattagt cgataaatca
    35041 accgtacaag ttccaataac ctgaccagct tttaataaaa tatatagtcg gctttctgga
    35101 tctttcagag cttcagcgac atatctatcc acaacttctc tcgattcatg ttcctctgaa
    35161 aatgcctgaa attttaattg actaatttga tcctgatacg aactatctgc taacaaaact
    35221 tcaagatggg aaacatttgc taacggataa ggtcttctat ccttacctaa ccaagtttct
    35281 gtctcttcat cctcgattag tccccagtta ctggcaaagt caggatgatt ctctaaaaaa
    35341 atacgttctg tctgaaaagt gactgaccga atggggaaag aagctgtttc tctctcaaaa
    35401 ctagtaaaca atgcacgcgc aatcccctga cggcgatgac ctggatgaac cagtatcgtc
    35461 acttctacat cttggtcatc tgcatagaca gttaataaac caacaagttc gcctttttca
    35521 taataaagga aaaaggcggg catgtttggg tcaaaattaa gcatgttaga gagataggga
    35581 tcgcgatagg taccgtcata gttttggcaa cagttaatta cttttttcgc ctcagatagc
    35641 tcctcttggc ttaacttgtt tcttgcttga atcatatagg tatcctctac aaaccagacg
    35701 atctgtgact ggcatcttta gcctgctcga gtttattgac ataatactct cgtttttctt
    35761 cgacttcgtg aatgacaggc tcatctttct taccatgaag acggacaatc ttggccggaa
    35821 taccgacaac cgtcacgtca ctaggtacat ctgctacgac aactgctgca gcaccgacct
    35881 tggcattttc accaatttcc acaggcccga taacttgggc atgggctgat atgagggctc
    35941 cctttcgtac agtcggatgg cgtttgccac agtctttccc tgttcccccg agagtcactc
    36001 cgtgatagag aagaacgcct ttttcaacaa tcgctgtctc tccaatcacc agaccagaac
    36061 catggtcaat aaaaacacct gaatcaatct gggctcctgg atgaatctca atctgagtcc
    36121 aaaagcgcca aaactgactg tacatacgag ctaatagttt gaagccgtgc ttccagagaa
    36181 aatgcgagag acggtgggcc gccaaggcct tgacacctgg ataagtcagc aaaacctcca
    36241 aagtggtgcg ggccgctgga tcattttctt ttacaatatc aatggtttcg cgccaccacc
    36301 ccatacattt ctccttttct tattctgaat cttttgatgt ttctgtaaat tctttcttag
    36361 gtttgtaatc cttttgatga cgtgggcggt gagggcgctc agacttttca cctttttcat
    36421 catgctcagg ttttggcgga cgaggtagaa gagccttcat agaggcatcg atacggcctt
    36481 tttcatcaat tttgataacc ttaacatcaa cttcatcccc gatttctacc aaatcctcta
    36541 cacgattggt acgagtccaa gccatctcag agatatgaac aagggcatct gtcttatcaa
    36601 agaggttaac aaaggcacca aatttctcga tacgaacgac tttagcacgg taaacttcat
    36661 ccactttggc ttcacgaacc aaaccagcaa taatttcttt ggcacggtta atagcatctt
    36721 ggtcactaga gtagatagac acatttcctt cttcgtctat atcaatctta acacctgttt
    36781 cagcgataat cttgtcgatg gtttctccac ccttaccgat gacaatctta atcttgtcca
    36841 catcaatctt gatcgtatca attttcggag cagttggagc caattctgga cgaacttctg
    36901 gaatggttgc ttcaatgaca tcaaggattt caaaacgcgc tttcttggct tgagcaagag
    36961 cctccgtcaa gatttctgca gtaatccctt gaatcttgat atccatttga agggctgtaa
    37021 tcccatcacg agtacctgca accttgaagt ccatatctcc aaagtgatct tccaaacctt
    37081 ggatatctgt caatactgtg tagttatttc catctgagat aagccccata gcaataccag
    37141 ctactggcgc cttgattggc acaccaccag ccataagggc aagagttccc gcacagatag
    37201 aagcttgaga tgaagaaccg tttgattcca aaacttctgc tactagacgg atagcgtagg
    37261 ggaattcttc caagcttggc aagacttgag caagagcacg ctcaccaagg gcaccgtgac
    37321 cgatttcacg acgacctggc gcaccgtaac gacctgtttc ccctacagaa tattgaggga
    37381 agttatagtg gtgcataaag cgtttcttgt actctggatc caaaccatca atgatttgag
    37441 tttctcccat cggagccaag gtcaagactg aaagagcttg agtttgccca cgagtaaaga
    37501 gacctgaacc atgtacacga ggaaggaagt caacaaccgc atccaaagga cggatttcat
    37561 cgaccttacg accatcagga cgcaccttgt cttctgtaat taaacgtcgc acttctgcgt
    37621 gttccatttg ttccaagatt tcagccacat cacgcataat acggtcaaat tcttcgtggt
    37681 ccgcatattt ttcttcgtaa acggcagtca cttggtcttt cactacttga gtcgcagctt
    37741 cacgggccaa tttctcttct acttgaactg ccttttggag gtcactgttg taggctgcaa
    37801 tgatttcagc ttgcaattca gcatccacgt gaagcaattc cacttctgct ttttctttac
    37861 cgacagcagc aacgatttct tcttggaagg caatcaattc tttgacagct tcgtgccctt
    37921 taaggagcgc ttccaacatg atttcttctg acaattcttt ggcaccagac tctaccatgt
    37981 tgatagcgtg cttggttcca gctactgtca attcaagaag agattgctct gcttgttctt
    38041 gacttgggtt gatgatgatt tggccatcta catatcccac ttgtacccca gcaattggtc
    38101 cgtcaaatgg aatatctgaa atagacagtg ccaaagatga accaaacata gcagccattg
    38161 gtgcagatgc attttcatca taagaaagca ctgtattgat gacttggact tcattacgga
    38221 aaccttccgc aaacatagga cgaatcggac ggtcaatcaa acgcgctgtc aaggtcgcat
    38281 ctgttgaagg acgtccttca cgtttcataa agccaccagg aaacttccca gccgcataca
    38341 ttttttcttc gtagttgact tggagtggga agaaatcccc agttgccatt ttcttagaca
    38401 taacggcagc agtcaagaca gttgactcac cgtaacgtac gacaacagat ccatttgctt
    38461 gcttagcaac ctgaccagtc tctacaatta actcacgacc cgcaaaagtc gtttgaaaca
    38521 cttgttttgc cattttaatc ccctttggat tgatgaaatt atacgccttg cctacaaaga
    38581 tcaagatacc aaggacgtca aaagcaaagt aaaaatagga aactgacgaa gtcttcgatg
    38641 aagacaagac agtttatctt ttttacacag cttttcggcc gtgttcaatt acacaagata
    38701 ttttggacgg ttcggcttgc cgaacatttc tgtagacaaa taggaaggtg acgtcgcact
    38761 cgacgagtgc taggaagctt atcttttttc ctaagaaatg agaccaaaat tcaagtcatc
    38821 aagataccaa gccgtcaagc aactcaaagg aagataggaa atcgaacgac ggagcgacta
    38881 ctcctaggga gatttatctt tttccacaga gttgtaggca agttcagttt tcaagataca
    38941 tcattagaaa ggtttaatac taaagtatct aaagctttca cgctaatcgc tatcgggcga
    39001 ttagctaaat gctttactaa ctctctcgtc aaataacatc gatttgactc actcgtgtcg
    39061 ttaaatctta cagtttaaat gcattgtatt atttaatacc ttcatctttg tatcaagtac
    39121 gtacagaatt tattttatca tatttttctt aaaaagtgag gtctttacca ttaaaaagga
    39181 accattcccc tcacctgaga agaatggttt gcttttatta tcctagagac tggtgattaa
    39241 acaaggcatg ggttgcttga tggatgtatt ttgctgtatc agcattattc atcgtataga
    39301 gatgcacacc ggcaacatcc tgagttacca agtccacgat ttggtccact gcataggcaa
    39361 gtcctgctgc tctgagcgac tcagggtcat gctcatactt gtctaagatg gctttaaatt
    39421 tgcgtggaag atggatattc tcacaagtct tcaagagtcg gagagcctga tttcgattca
    39481 gaattggcat aattcctgca tgaatgggaa catcaatccc agccaagata cacttgtcct
    39541 gaaaatcata gaagcgctca ttgtcaaaga agagctgagt tacgaggctc gaacagcctg
    39601 catccacttt cttcttaaga ttttgaatat ctgaaatctg atttggcgaa tctggatgcc
    39661 cttctggata gcaagctcca ataatatcaa agtgaggggt ttgttccttg ataaactcaa
    39721 tcaagtcggt tgcatagcgg aaatcctttt gtggttccac gtctggaata atatccccac
    39781 gaagagccaa gattttctgc accccaactt tgtccaagtc agcaatagtt tcagcaacct
    39841 tgtccttagt tagataaata gctggcaagt gggcaatggt cggaatcgcc aaatcatttt
    39901 ggataaagtc agccaaacga accgtcgttt ccttgatatt aaatttatta ttgctggcag
    39961 ttacactgat aaaatgggga gccaactcct gcatatcctg caaggctgaa ataatgttat
    40021 cattacccac ggctgggttt ggagggaaca cttcaaatga gagtgacggt gtttggcgtg
    40081 acatatgtaa taaccttttc tagttgattt ctttttgaac aaccactgta tggagagaaa
    40141 tccaatctta caatttctca cgcgcagctt tagctgcttc aacaaggcgg atcaagcttt
    40201 cttttgtttc tgggatacca cgtgttttca aaccacagtc agggttgatc caaactttct
    40261 tgcttggcac tttagcaagg atggcttcga ttgtgttgtc gatttcgcct tcatttggta
    40321 cacgaggtga gtggatatcg taaaccccag gtcccacttc tgtttggaag tttttcgctt
    40381 tgagttcgtc caagatttca aggtttgaac ggttagcttc aaaggaaata acgtctgcat
    40441 ccatgttatc gatagctggg atgatatctg taaattctga gtaacacatg tgagtgtgga
    40501 tttgtgtgtc tggcgctact gttgagtgta ccaagcggaa ggcaggaata gcccagtcaa
    40561 ggtagtcttc gtaccagtcg ctacggcgga gtggcaattt ttcacgaaga gcagcctcgt
    40621 cgatttggat gattttcaca ccagcagctt caaggtcaag tacttcatcc ttgatagcaa
    40681 gggcgatttg gagagttgaa tccttgatag agatgtcttc acgtgggaat gaccagttaa
    40741 ggatggtaac aggtccagtc aacatacctt taacaggttt gtttgtacga ctttgtgcat
    40801 agctagacca tttaacagtg atagggttaa gacgagtgac atcaccccag atgattggtg
    40861 gttttacccc acgcataccg tatgattgta cccatccatt tttagagaag aggtatcctg
    40921 acaagttttg accgaagtac tcaaccatgt cattacgctc aaattcaccg tgaacaagga
    40981 catcaaagtc aatatcttct tgccacttga tccattcgtc aatcgtttca gcaaggaaag
    41041 cgtcgtactc tttttgagac aattcacctt tacggtaagc caaacgtttg gcacgaactt
    41101 cttttgtttg agggaatgaa ccaatcgttg ttgttggaag agctggaagt ttgaaagctt
    41161 cttcttggat agcttcacgt tctgcaaagg ctggcaaacg agtgtagtct gcgtctgtca
    41221 agccagcgat acgcgcacga agttcagcat tttcaccaac acgctcagtc gcaaagagtt
    41281 ctttgttggc tgcaagagct tctgaacctt gaccatttcg gatagcatcc aagtcacgga
    41341 tttcatccaa tttttcaact gcaaaggcaa agtggttcaa gagtgctggt tcaaattctt
    41401 cattagcagt tgtaaatggc acatgaagaa gtgagcaaga gcttgtcaaa acgatgtttt
    41461 cagctggaat ttgctcaaga acagccaagc tcttttcgta gttgttgcgc cagatgtttt
    41521 taccattgac aatacctaca tagagagtct tgtcagctgg gaagccacct ttaacgagtt
    41581 caagagtttt cttaccttca acaaagtcaa gaccgatagc atctactggt aagtttacaa
    41641 ggtcagcgta tacgtcacga acatcaccga aataagtttg aagcaagact tcaagacctt
    41701 ttttgtcagc caagagtttg ttgtaaaggt tcaagaagag agctttttct tcagctgtca
    41761 agtcttttac aagagccgct tcatccaatt ggatgcgagt cgcaccaagt tcagccaatt
    41821 tagcaaaaac ttcttggtaa gcagccacta agctatctac gaagtcgtct gctttcacgc
    41881 cttcttcaaa gtctgacaat tgaaggaaag tgaagggacc tacaagaaca ggacgagtgt
    41941 tcaatccaag ttctttggct tcttggaact catcgaaaat cttgtgacca gccaatttta
    42001 cttgagtgtc tttttcaaat ttaggaacga tgtagtggta gttagtgttg aaccatttct
    42061 tcattggaag ggcgcgaacg tccccttttt ctccctggta accacgtccc aaagcgaagt
    42121 agcgctcaag gtcagacaag tccaagtttt gaacggatgc aggcaccacg ttgaaaagga
    42181 aagccgcatc taggaagtta tcatagtgag aaaagtcatt tgatggaatt tcagtgatgc
    42241 ctttttcttt gacaatgttc cagtgtttag cacgcaagtc ttttgctgct gctaaaagtt
    42301 cttcttctga gatttctttt ctaaagtatt tttcagttgt aaattttaat tcacggaatt
    42361 cgcccaaacg agggaaaccg atgattgtag ttgacatgat gtgtcctcca aaatttgttg
    42421 ttgaaactat cttaacagaa aagaaagcgt ctgtataatt gtaaaaaatt agggtttgat
    42481 atagtttgaa actatatatc tgtttcggac aaaagaaaaa gacttgaagc aaacgtctca
    42541 aatcctttgt aattcttact ttacagctat attccaatta gaatactaaa acatgttatt
    42601 agtaattctt ataagtgact atgacctgtt attagaaaag actataactg attctagtca
    42661 actttttccc tgttcaagtg ggacgattgc tagtgtcttt cctaaactgg ctaggacttt
    42721 taagactgta tccaactgag gactagtctt tcctgtctcc atcctagcta tgacaggctg
    42781 gcttattcca ctgacttctt ccagcttttt ctgactgatt ccttgttcat acctagcctc
    42841 aatcaactcg ctcatgatag ccactcgcat atcactttca aggatttcct ccttgctaaa
    42901 gagctcagat ggacatcctt ccaattactc ccaatagcac tattcttcat cacttaaccc
    42961 tctttttttt acgtctatgt atttttaaaa aaatgagcga attatgattc gatagattga
    43021 ccagtgggtt taaagttggt gctagcctat ttcttaagcg attttccttt tctaggataa
    43081 agcagttcct gcttgcttaa ccccaatttt ccacgatgaa tccaatagta aatggttgaa
    43141 attcccacgt taaccccttt agccatcacc atcatttcag gcgaaaattt ttggttatgt
    43201 ttttggttat gtatagtgga gaatcttttc ctttagtttc ttaagactgt tgagcgtagt
    43261 cggcagaata aatctctttg aagcgccctt ttccaagaca ttgtcggact gtcccacgct
    43321 tgatttcagt gtggatagtt tgaggagctt ttccaagtag agaggcaatt tctctatttg
    43381 attttccttc ttttttccat cgttcgatta agcgacggct atcgattgtc aaatgtttgc
    43441 cttttgtagt ataattgtct tgcatttctg tgccttttaa tcatttcaat cttaaattgg
    43501 acttttttta cttgggttgt acttaatcta tgaggaagac aagaaaaaga atatcaatca
    43561 agtaaagtca caaagtcaca ttagctccga gcaaccattg caaattgagg tactcacaca
    43621 atgattaaaa catttctctc tgccctttcg gtcattctct tttctatccc tatcataact
    43681 tattcttttt tcccatcttc taatcttaac atttggctat ctacccaacc tatcttggca
    43741 cagatttatg ccttcccctt agctactgca actatggctg ctattttaag tttcttattt
    43801 tttttcctat ctttttacaa gaaaaataaa caaatacggt tttactctgg cattttgctc
    43861 ttactatcgc tcatattact attattcgga acagataaaa ccctttcttc tgcatcaaat
    43921 aagactaaaa ccttaaaatt agtaacttgg aacgtcgcta atcaaataga agcacaacat
    43981 attgagcgaa tttttagcca ttttgacgcc gatatggcta tattccctga actagctacc
    44041 aatatcagag gtgagcaaga aaaccagaga atcaaactat tgtttcatca agttggactt
    44101 tctatggcca actatgatat tttcacttct ccacctacca atagtggaat agctcctgtg
    44161 actgtgattg tcaagaaaag ttatggtttc tatacagaag ctaaaacttt tcatacaaca
    44221 cggttcggga caattgtatt acattcgaga aaacaaaata taccagatat cattgccttg
    44281 catactgcgc ctcctctgcc aggtttaatg gaaatctgga agcaagactt aaacatcatt
    44341 cataatcaat tggcttcaaa atatccaaag gctattattg caggtgattt taatgcaact
    44401 atgcgtcatg gagcacttgc aaaaataagc tctcataggg acgcattaaa tgcactgcca
    44461 ccttttgaaa gaggaacttg gaatagccaa agtccaaaac tttttaatgc aacaatagat
    44521 catattttat tgcctaaaaa ccactactat gttaaagatt tagacattgt aagttttcaa
    44581 aactctgatc atagatgtat ttttacagaa atcacatttt aattatttta tataaaatca
    44641 cccctctaat gttcataaac tagaggggga atttgtatcc tactatcgtt taacgcactt
    44701 ctgcattgac tttttcttcg agagacgctt ggattttttc catatagcgt gcgacttctt
    44761 cgtccgttaa gctgtcttct ggattttgga aggtcaagct ataagccatt gacttcatac
    44821 caagtcccag tttttcacct gagaagacgt caaagagttt gatatctgtc aaacgtttca
    44881 cgccggcagc ttggatagca tctacaactt cttggtgagt cacttctgcc ttgaggagaa
    44941 gggcaacgtc acggctgact gctgggaatt tggtgatttc cacaaatgga acagcaggtt
    45001 ggagcgcccc ttcgatggct gaaaggttaa gctcagctac atacgtttct ggaatatcgt
    45061 aagccttggc agtgactgga tgcacttggc caaggaaacc aagaacttgg tcaccgagtg
    45121 aaatcacggc tgtacgacct ggatgaaggc taacgatttc agatgttgct gtataggtta
    45181 cttggagtcc caaacgagta aatagggctt caaggattcc cttagcatag aagaaatcaa
    45241 ctggaactgc tgctgtttgg aaatcttttt cagcaaccaa gcctgtcaag gcaaaggcaa
    45301 agctgttgat ctcatttgga agttcttctt ttggattacc tgtttgttca aagacttttc
    45361 caatctcata aagggccaag tttttattct tacgagccac gttgtaggca acggtatcaa
    45421 ggatccctga aatcatattt tgacggagga ctgaacgatc cacagtcatt ggccacatga
    45481 gttcagtaag gttacttggt tgagctgtga actcaactgc tttttcagga gttgtcagag
    45541 cataggtgat gatttctgtc aaacctgctc cttcagcaat ggtacgaact tgacggcgga
    45601 gtttttgtat cacagtcaat tcaccagctg taccatcgtc ttttggaagg ctggttggca
    45661 agcggtcata tccatagata cgagcgattt cttcaaagag atcagcttcg attgtgatat
    45721 cccaacgacg acgtggtacg ctgactgtaa agctgtctgc atttccagaa agaccaaagc
    45781 caagacgacg gaagacgtct tctacatcag cataagacag ctcagttccg aggacacggt
    45841 taacatcagc aagggttgaa gaaacttcca catcagaggt atcaagctca cccgctgaaa
    45901 cgataccctt acgcaccgtc gcgcctgcaa gctctgcaat catgctagct gccgcatcaa
    45961 gggcttcatt aactgttgcc acattaattc ctttttcaaa gcgagaagat gactcagaac
    46021 gaaggttcag gcgaccactt gtcttacgga tagatttgcc attaaaaaca gcagcttcaa
    46081 ggataacacg actagatttt tcagaaattt ctgtagcctg accacccata acaccggcaa
    46141 gggctactgg tttgtcagca actgtaatca cgaggtctgt ctcagccaag tctcgttctt
    46201 caccgtccag ggtcactaat ttttcaccat cacgcgcttc acgcacacgg atgtcagtcc
    46261 cttcaaatgt gtccaagtca aaagcatgca taggttgacc aaagtagagc aggatgtagt
    46321 ttgtcacgtc tacaacgtta ttgatgggac ggatgccttc gttcatgaga aggttttgca
    46381 accattgtgg acttggtgcg atagtcacat tgtccaagat acgagctgca tagtaaggcg
    46441 ccttgtctgt ctcaatgctg acagaaaggg catctgccgc agcttcatta gtttctgtta
    46501 gagtaaattt tttaaagttg actgccttgt catagatggc tgccacttcg tgagccactc
    46561 cacacataga aagggcatct gcacggtttg gtgtgatgga aagttcgatg atttcatcat
    46621 ccaagtctag gtaagaaaag acttcctcac ctggcacggc atcttcaggc aagatttgga
    46681 tgccatctgc gaattcctta ggcacaactg agtcagaaat tcccaattca ccaagtgaac
    46741 agatcattcc aagtgactcc aaaccacgga tttttccttt tttgattttg tagttatcag
    46801 cgatacgagc tcctggaaga gccaccatga ccttgatccc agcacgcaca tttggggcac
    46861 cacaaacgat ctgacgctct tcttcttcgc caacgttaat ctgacaaaca tggaggtgag
    46921 tctctggcac atcttcgcaa gacaagacct caccgacgac aatttttgag agaccagcag
    46981 ctggtgattc gacaccctct acctcgatcc ctgtagttga cattttttca gccaactctt
    47041 gtgatggcac atcaatgtcc accaattctt ttaaccattt ataagataca agcataattt
    47101 agttctccag aatgacagtt gtcactctag ttcttttcct ttcctatcat ttcaatagaa
    47161 gaatcctctt cttaccttaa tttctttctc agtaaccaat ccgtatctac tttttgacca
    47221 accataaaat gatgttggct aaatttttca aaaccatatc ggttataaaa cgcttgagct
    47281 tttgtattat gctcccaaac acctagccaa gcccaagaaa aactattttt tgtagcaagt
    47341 tcaagtgcga attcaaacag ttgcttacct agtccaaatc cttggaattt ttgtagcaca
    47401 tagagacgtt gaatttcaaa agcgtcctct aattctctct cagtttgagc acttccccag
    47461 ttgactttga gaaaaccagc tatctcctcc tcatgcataa tgaaataggt ttcagagtca
    47521 ggatttccca actcagttga caaagttttc agactataag cctcttcaaa gtattcctgt
    47581 aactgctctt ccgtattatc atacgcaaag gtttcacgaa aggtttgttt ggcaatttta
    47641 gccaacacct caacatctgc catttctact tttctaatca ttatttaaac tgttctgaga
    47701 agcggacatc tccttggtag aatccacgga tatcgttgat tccataacgg agcatagcta
    47761 cacgctcttg tccaagacca aaggcaaagc cagagtatac agtcgcatcg ataccactca
    47821 tttcaaggac acgtgggtga accataccgg cccccataat ttcgatccaa cctgttttct
    47881 tacatacatt acagccttct ccaccacact tgaagcaaga aacatccacc tcaacagatg
    47941 gctctgtgaa tgggaagtaa gatggacgca aacgaatttg acgctcttca ccaaacattt
    48001 tttggacaat caactgaagc gttccttgaa gatcagccat agagatattt ttcccaacta
    48061 ccaagccttc gatttggtgg aattggtgac tgtgggtcgc atcgtccgta tcgcgacgga
    48121 agacacgccc tggcgagatc atcttcaaag gacctttaga aaaatcatgg gcatccatag
    48181 cacgcgcctg aactggagac gtgtgggtac ggagcaagat ttcttcagtg atatagaaag
    48241 tatcctgcat atcacgagct gggtggtctt ttggaaggtt catacgttca aagttatagt
    48301 agtcttgctc cacttcaaaa ccatccacga cttgataacc cataccgatg aagatatctt
    48361 cgatttcttc actggtttgt gtcaaaacgt gacggtgacc agtcgcaact ggacgacctg
    48421 gaagcgtcac atctatactc tcgctagcca gttgagccgc gactttcttt tcttccaaga
    48481 gcttagctgt ttcttcaaaa gcagcagtca agacatcacg agcttcattg acgtgtttcc
    48541 cgatgattgg acgcatctca gcagaaacat ctttcatccc tttgaggatt tcagtgagcg
    48601 aacccttttt accaaggaca gagacacgca aatcttgcat ctctttttca tttccagcag
    48661 taatctgctt caagctagcc agcgtttctt cgcgaagcgc ttttaattgt tcttcaatag
    48721 ttgacatatt tcctccatca gtctctcgta gataaaaaga aaaccacatg ccaaaaactc
    48781 cactcggagc gttgacacgc ggtaccatcc gttttcatct gacaagtcag accttcattt
    48841 ctaaatccat gcgcaagtga attcacccag ctttcatata gagagcttgc agtcacggct
    48901 ctcctccctg atatacttcc cttgggctac tagtctttca gattcctatt caattactac
    48961 ttagtttatc agatttttac cattcttgca agacctatct tacttctgct tgttagctta
    49021 ttcttatcta aatttatata aacaatttat ctaaattaac tatttataat ttttgtaaca
    49081 aaattaaatt aattgacact cccctataaa ataaagaagt ttagaattta atgtcttcca
    49141 aacttcttta ttccatattt aatgaaatgc caccttaacc gtgataatag ctagtcatca
    49201 ataaaaaact atttgaataa ggattctcca tttgattcaa tcacttcttt ataccaagta
    49261 aaagacattt tcttatatcg atttaatgta ccacttccat catcgtttcg atcaacataa
    49321 atgagaccgt accttttaga aagttgtgca gtggacatag aaacacagtc aatacatccc
    49381 caagacgtat agcccataat ttcaacacca tcctgtagag cttcagcaac ttgcaataaa
    49441 tgttctttca tatactgaat tctataatca tcttggacgg ttaagttatt aagttcatct
    49501 tttattagtt gatctttagc acctaatcca ttttctacta taaataatgg gatttgataa
    49561 cggtcataat atctatttaa aattatacgt agtccaattg gatcaatttg ccatccccac
    49621 tctgaagact ctaaataagg atttactaaa ccaccaataa tattcccttc tcctgaatta
    49681 tactgtgttg gaagagcaga ttgagtcaca ctcatgtaat agctaaagga taaaaaatct
    49741 acggtataat tttttaataa ctctgcatct tcagctgcaa actctatgtt aatgtcattt
    49801 tccttaaaat atctttttgc ataattcgga taataacctc taacatgcac atctgaaaat
    49861 agataattta gattctcata ctcatgagtc gcccatacat cttttggatt tggagtcatt
    49921 ggataagctg gcatagctaa taccatacat cccaccttaa actctgaatt aatctcacga
    49981 gcaatttttg taaccaaact tgaggcgact aattcatgat gtatagcttg atataattct
    50041 tgtttcgaaa gattctcctt aggtatatct attcctccac tagtaaatgg taattccaaa
    50101 acagagttta cttcgttaaa tgtaagccaa tatttaactt tatctttata cctttctaaa
    50161 actgttcgag caaatttttc ataaaaatga atcattctcc tatcaaccca tccatgatat
    50221 tttcttgcta aatataatgg agtctcatag tgtgaaagag ttacaagtgg ttctatcccg
    50281 tgagcatgta gttcatcaaa caattcatca taatatttca acccagcttc gttaggttct
    50341 tcctcatctc cttttggaaa aattctactc catgcaatag aagtacgaaa aacattaaag
    50401 cccatttcag aaaacaagga tatatcttcc ttatatttat gataaaaatc aatacctatc
    50461 aattttaagt tatcttctgt aggattttct gttgcttctc ctaatccacc tttgggtaac
    50521 acatcctgaa ctgataagcc cttaccatct tcattatatg ctccctctac ttgattagct
    50581 gcaacagctc caccccaaag aaaatcatct ggaaaaatgg tcataacttt cctccattat
    50641 aatattacca gtaattcctt agaatgctcg attgtctgat tattaggtaa tactaataca
    50701 tctagaaaat cattggtatt cgttacaatt actggtgtaa ctgtttcgta gcctttagtc
    50761 ttgattaaat tcaagtccat ttcaaaaatc aactgatttt tgaaaactct gtctccttct
    50821 tctacatgac taataaaacc ttgacctttt agctcaacag tatctaatcc aatatgaatt
    50881 agtaactcaa caccctcatc actcttcaat ccaattgcgt gcttagtcgg aaaaatattt
    50941 gtaattttcc catcaaatgg tgcataaacc ttaccttcac ttgggataat cgctactccg
    51001 tctccaatta gtttatctga aaatgtttta tcctggacat cgcttaacgg aatgatttct
    51061 cctgatatag gagaaaatat cattttttta tttgaaactc cagcttcaac ttctaaattg
    51121 ctagaactct cttcttcatc gattccaaat atataagcta atacaaaggt aataacaacc
    51181 gaaatgaccg ccacaattaa agcatttaca atatttgatg gcacatcaga ataaataaat
    51241 tgaggcaacg ctatcaaaga tgggacagca aatagatatg ctttaacact agtaagacct
    51301 gcaaataatc ccgctaatcc accaccaatc atagctgcat aaagcggttt tttatatttt
    51361 aaagtcacac catataatgc aggttcggta atccctgcaa gtaaggctga gaaacctgct
    51421 gcaaaagcaa tttgttttgt attattattt ttactcttta atgcaacagc catcgaagca
    51481 gccccttgag ctaagtttga ccctaacatt gctggaagaa ttaatacgtc tggagtagca
    51541 atagatgccg ccaaaaaaat aggtgcaaaa gcccaatgca ttccagtcat aacaataaat
    51601 ggcataatag caccaagaat agctaatgta agccatccag ctacaccata catttgccca
    51661 actagatttg ataatccttc accaacaatt actccaatag gtccgactac aactaaggca
    51721 atacagcttg atactaataa tactagcgta ggttgcaaaa aactcttagt aatagctagt
    51781 gttaatttag caattatttt ttcaatatat ttcatcaacc aaaccataat aagaattgga
    51841 acgactgatg aaccataact agctggtgtc acaggtgcac caaataaact aagaggattc
    51901 cctgattgca ccatttgaac aaaatttgga tggagaagta cacctgctac agacatagct
    51961 aatgtagatg ttacttttaa tttttgtgat gcagaataag ctaataacag cggtaagaaa
    52021 taatatggag catccccaaa aaatgtcaaa aaagcaatag tctgagaatc tgattgcaat
    52081 ataccaagca ttggtaaaat gattaccaag actttcaaca tacctccccc taacattgct
    52141 ggaatgattg gagtcatgga accagcgata tactcaatga ttctttctaa aatattccct
    52201 ttgtgccctt gaacaactga atcggattca aaattgccaa gtttaacgaa ttctttataa
    52261 taattagcta catcattacc aagtataatt tgatattgtc cattcttttt cataatacct
    52321 attacacctg gtatcttctt cacatcatca tcattgacta aattttcatc ttttaattct
    52381 aatcttaaac gtgttacaca atgggtaact ctattgacat ttttttcacc tccaattaca
    52441 tcgaggattt tttgtaccgt atctttataa ctcatggtat tctcctattc tattaatcta
    52501 aattttttgt taagcgacga atatgagcca tcaaataaac taattcacta gaagtcagca
    52561 aataattgta ctccgtttgt ataaacattg ctacctgttc accacattca tattctctag
    52621 gatatttatt tttcattaat gctaacaagt cttcatcatc atcgtcacaa taagttgtat
    52681 tttcaaccaa gcgatgggca aataatttta tatgagttat gaatctataa taatcaatag
    52741 aatcttggtt aaactcagta caatagaaat cttgaatgat tttctcaatt cccataatta
    52801 tttcagtgat tttatacgac tcttgaaaat tattttctaa attagcatta acaaaatgta
    52861 gcgctatgaa tgcagcttcg tcaattggaa gttcaatacc taactctttt tttattattt
    52921 gtaaagccct tttcccaagt tcaaattctt ctttataata tttcgatatt tcccagcgta
    52981 ggggattcga aataataatc ccttctttat aacgttctat gctcgaagaa atatgatccg
    53041 ttaaattaat atagataatt tcatcaaaat tattacccaa tgttatttta cctaggttaa
    53101 ttattttttc ggtgatgtac accaactcca aaggaacgtt aataaacatc tctgtaaaac
    53161 gggtcatatt atctctattt ttgagaaaaa aacttttctc aatatcaatt ggatttactt
    53221 tatctccagt ttttcttcca aaagctattc cccttccaaa aagaagaata tcgatatctt
    53281 ttttactttg cgcaattacg gcattgtggt ttagtattcg ttttatgatc ataaaatttc
    53341 ccttttaaaa taaaaggaac ctacttaaaa acatcaagaa acttcttctg atgtgattaa
    53401 ataggttcct gctcacttgt attagtgata acatcctttt atttcaattt ttaatggtga
    53461 catgaagctt aaataattaa taactctgta accgttttta ttttatgata tttcaatcta
    53521 tttgtcaagt acttataaaa attttttatt ttgtgtgatt cgaatgtgat aatggcaaca
    53581 gtctcactaa agaaatgaaa atcatacata gaaattttta aaaattttct ctatcgtaaa
    53641 cctgccacta gttcagaaac tatactagct gattccaaac gtaatatggt catctcatct
    53701 accttcccta ataatggcat tgcaccatac caaacaatag tcttattcac caaaagaata
    53761 ggcgttacta tttttgattg agtaaaagac actttaattt tctggctctt caactggtct
    53821 agccatgctt tatttgcaac cgtctctggt atacatatct caatttgact aatcctaaat
    53881 tcttttagaa aattcatcag ttttgtctgg tgcacataag gtaaaataag cagacattcc
    53941 tgtctttccc ctgctaagtc ctctctcaac accttctcat atctactatc aacataaacg
    54001 aattgtttct cctcaccctc gatgacacga taatccatct ttcgataagc tacttgtcgt
    54061 ttctgaaaca tcttttctaa ataaggaaca tgaatatcca cataatcgaa aatacgcacc
    54121 aaagacttat ccttgtagtt tctatgaatc cgacctgcat actgaatcaa attatttttc
    54181 caagaaaagg gtgctgccaa gataagcgtg tccaactgag gtaagtcaaa accttcgcca
    54241 atgtattttc cagtagacaa caaaacaaac cctttatcta actgttctaa cgtctccagt
    54301 aaactcgttc tctctcggac tttggttttt ccgctaataa tgtaacagtc atcaacctct
    54361 ttctctttca ataatttttc aaagacatct atctgttgaa ttcgattaac taaaaccaag
    54421 atattccgtc cttctgccac ttgggctaga atatccttga gaatcatctg attcctcact
    54481 gagtcagtag caatccaatc actaagctgt ataaaattac ttgctttggt cttttcaatt
    54541 tccaaatgac caaaagaagt gaatcttaat tgcaattgcc gtttaaaatc cgtttccctc
    54601 ttatcagcag tatggagtat ctcaccaatt ctctgaaaaa caataggctc atgaccattc
    54661 ttacgctcag gcgtagccgt caaaccgtaa agatacttcc ctctaaactg agcaacaact
    54721 ttttcaaaca tcaaggcaga gacatgatga cactcatcca caatcatcat ctcatactca
    54781 tccaaaagac tttgactatt ttccaactta aatagagatt gaatcataac gacatcaacc
    54841 agtttactca gccatttctt agtcccaccg tactgcccaa catagccgat taccttttca
    54901 cgacctgatg ccgtataacg gatagcctcc tcctcttcga aagtcaaaaa gcagtttaag
    54961 cgatctagcc attggtctaa gagttgctta ttatggacta gaataattgt ttttgttttc
    55021 cgttcagaga taagagcagc acctaaaacg gtcttcccaa aaccagtctc cgcatgaagt
    55081 aaaccatttt ctttagaagt catatctgac agggctaact cttgctcaaa agtgagttct
    55141 cccttaaatt ccacgctaat agacctttgt acctttctcc tatcttccac agatacctgc
    55201 ttaaatttat cttgcaatgg atacagcaaa cctcttggca accataaata atgatcggat
    55261 tctccaaata aatacattct ctcaggaatt tgataggttg gctgtcgcat agcctgcttt
    55321 aaatatagta aaatgaaata ataacaggac aaatcgatca ggaaagtcaa atcgatttct
    55381 aacaatgttt tagaagcaga ggtgtactat tctagtttca atctactata aaattcggga
    55441 ttagaaaagg aagccatatt tttcaagaaa aaagtgcctt ggatgtcaca gacgattttt
    55501 caagttggat catattggat aaaacgacct tcaactcctt atccaattct tgcttgccta
    55561 actcctcttg gattaacagt gccactttag cagttgaaat cctctgaatt tcttgtagat
    55621 acctccattg gtcttcataa ggctgaaact gttcatccac aaagaccgtt cgcccttgat
    55681 ggtaagcttc tccttgaaaa ggcaaggcaa tcaaatttcc aaatcccccc ttaggaagga
    55741 catcctgatt tggaaacatg cgatcaaaag aatcaaagga cagttgcata ctttcctgca
    55801 ttgccagttc tatcagtttc tttccaaaca agcgagcctc tcgactcgga atcgcttcct
    55861 caaagaagaa ccaaatatgg agtccgtgac ccgaacgaga aatctctaaa tgggcttcca
    55921 tctggcgttc cctggctatt cttcgaatgg ttaaaccagc ttctttccaa tctccttcat
    55981 ccaaatccag taccaaaaaa taacagctat catctaagtg cataggaaag ataccgatag
    56041 ctgcctcccc acgaaaatga gatttcaaaa cggagttcgt caatgtctgg aaactccgtt
    56101 tttcaggtgg caactgcttc caaccataat cataggaagg aaaatactga attttccctt
    56161 gttcattgat aaaattctta gcataaacat cataacggcc agcaaacaca gaggtaaaca
    56221 attgtaattt ttcttgcgtc gtcaagcgac tactttgaag ttggaacatc tcctcgaact
    56281 gagagcgttc cataacaaac tgactcttgt ctttaagacg tttatagtgg aatgaaataa
    56341 gatatgaaca aattgattag gaaaatcaaa ttaatttata gaaatctttt agcagtcatg
    56401 gcgtcctatt ccagattcaa aatgctatac acaagaaaaa ctcactatcc ccattaaaag
    56461 aagaaaagac atcaaggact tctaccacaa ttccatccaa agacacaaaa acagccataa
    56521 gagtcacctc cttgattcct ataggctgat tataacaaga ctggctgaaa ttgtacatga
    56581 aaataaaatc ctaatagtac tcattttgta tgtgactaat attccgtctc gctccagaag
    56641 gtacgaagta aatagagttt aaacgagcag tctataaaat tcaaaaaacg catattatca
    56701 agcgacctaa aactttgggc tctataatat ttgtagtggg taaattccct atggatatta
    56761 tggagcctat tttgggtaga aaaaaagtcc cataagatct ataatgaaaa gcgacaaaac
    56821 aactcattag aaagaatcat atggaacaat tacattttat cacaaaatta ctagacatta
    56881 aagaccctat aaaattcctt accttgaaac gactggtatg cctactagaa ttctccttag
    56941 aaaacgtcgt tttaagtgct atcattgctt gaaaatgatg gtcgctgaaa cttctatcgt
    57001 caagaaaaat caccaaattc ctcgtattat caaccaaaag attgcgcaaa agttaattga
    57061 aaagatttct atgaccgata ttgcccatca gctggccat
//
LOCUS       DBDESG010000004        52790 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.004, whole genome shotgun sequence.
ACCESSION   DBDESG010000004 DBDESG010000000
VERSION     DBDESG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 52790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.004"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            988..1440
                     /locus_tag="ACKB38_000239"
     CDS             988..1440
                     /locus_tag="ACKB38_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGL7979087.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1459..2796
                     /locus_tag="ACKB38_000240"
     CDS             1459..2796
                     /locus_tag="ACKB38_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7979088.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2875..3759
                     /locus_tag="ACKB38_000241"
     CDS             2875..3759
                     /locus_tag="ACKB38_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7979089.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3759..4592
                     /locus_tag="ACKB38_000242"
     CDS             3759..4592
                     /locus_tag="ACKB38_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7979090.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4610..6703
                     /gene="nanB"
                     /locus_tag="ACKB38_000243"
     CDS             4610..6703
                     /gene="nanB"
                     /locus_tag="ACKB38_000243"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HGL7979091.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6715..7818
                     /locus_tag="ACKB38_000244"
     CDS             6715..7818
                     /locus_tag="ACKB38_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HGL7979092.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDRAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7992..8690
                     /locus_tag="ACKB38_000245"
     CDS             7992..8690
                     /locus_tag="ACKB38_000245"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGL7979093.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8709..10226
                     /locus_tag="ACKB38_000246"
     CDS             8709..10226
                     /locus_tag="ACKB38_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGL7979094.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10351..11679
                     /locus_tag="ACKB38_000247"
     CDS             10351..11679
                     /locus_tag="ACKB38_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7979095.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11931..12797
                     /locus_tag="ACKB38_000248"
     CDS             11931..12797
                     /locus_tag="ACKB38_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7979096.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12813..13652
                     /locus_tag="ACKB38_000249"
     CDS             12813..13652
                     /locus_tag="ACKB38_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7979097.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13924..14376
                     /locus_tag="ACKB38_000250"
     CDS             13924..14376
                     /locus_tag="ACKB38_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGL7979098.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14473..14817
                     /locus_tag="ACKB38_000251"
     CDS             14473..14817
                     /locus_tag="ACKB38_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979099.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGTY
                     AAKYGISMARSILSRVAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14827..15036
                     /locus_tag="ACKB38_000252"
     CDS             14827..15036
                     /locus_tag="ACKB38_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979100.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFDRHTWSTILIASLFGV
                     YDYKPIDKNRKKSKRKNRFVHGRDL"
     gene            15094..15738
                     /locus_tag="ACKB38_000253"
     CDS             15094..15738
                     /locus_tag="ACKB38_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGL7979101.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15907..16824
                     /locus_tag="ACKB38_000254"
     CDS             15907..16824
                     /locus_tag="ACKB38_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGL7979102.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16842..17726
                     /locus_tag="ACKB38_000255"
     CDS             16842..17726
                     /locus_tag="ACKB38_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGL7979103.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17742..18593)
                     /locus_tag="ACKB38_000256"
     CDS             complement(17742..18593)
                     /locus_tag="ACKB38_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGL7979104.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18793..20850
                     /gene="pbp2b"
                     /locus_tag="ACKB38_000257"
     CDS             18793..20850
                     /gene="pbp2b"
                     /locus_tag="ACKB38_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGL7979105.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20861..21457
                     /gene="recR"
                     /locus_tag="ACKB38_000258"
     CDS             20861..21457
                     /gene="recR"
                     /locus_tag="ACKB38_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGL7979106.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21633..22676
                     /locus_tag="ACKB38_000259"
     CDS             21633..22676
                     /locus_tag="ACKB38_000259"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGL7979107.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNNITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22760..24133
                     /gene="murF"
                     /locus_tag="ACKB38_000260"
     CDS             22760..24133
                     /gene="murF"
                     /locus_tag="ACKB38_000260"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGL7979108.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            24120..24731
                     /locus_tag="ACKB38_000261"
     CDS             24120..24731
                     /locus_tag="ACKB38_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGL7979109.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24822..25526
                     /locus_tag="ACKB38_000262"
     CDS             24822..25526
                     /locus_tag="ACKB38_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGL7979110.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25745..27118
                     /gene="ftsA"
                     /locus_tag="ACKB38_000263"
     CDS             25745..27118
                     /gene="ftsA"
                     /locus_tag="ACKB38_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGL7979111.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            27135..28394
                     /gene="ftsZ"
                     /locus_tag="ACKB38_000264"
     CDS             27135..28394
                     /gene="ftsZ"
                     /locus_tag="ACKB38_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGL7979112.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28399..29070
                     /locus_tag="ACKB38_000265"
     CDS             28399..29070
                     /locus_tag="ACKB38_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGL7979113.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            29080..29619
                     /locus_tag="ACKB38_000266"
     CDS             29080..29619
                     /locus_tag="ACKB38_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGL7979114.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29619..29882
                     /locus_tag="ACKB38_000267"
     CDS             29619..29882
                     /locus_tag="ACKB38_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGL7979115.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29879..30664
                     /locus_tag="ACKB38_000268"
     CDS             29879..30664
                     /locus_tag="ACKB38_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGL7979116.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30673..31461
                     /gene="divIVA"
                     /locus_tag="ACKB38_000269"
     CDS             30673..31461
                     /gene="divIVA"
                     /locus_tag="ACKB38_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGL7979117.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31809..34601
                     /gene="ileS"
                     /locus_tag="ACKB38_000270"
     CDS             31809..34601
                     /gene="ileS"
                     /locus_tag="ACKB38_000270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGL7979118.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEYGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERATGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            35004..35389
                     /locus_tag="ACKB38_000271"
                     /pseudo
     CDS             35004..35389
                     /locus_tag="ACKB38_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35924..36616
                     /locus_tag="ACKB38_000272"
     CDS             35924..36616
                     /locus_tag="ACKB38_000272"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGL7979119.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36669..39080
                     /locus_tag="ACKB38_000273"
     CDS             36669..39080
                     /locus_tag="ACKB38_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGL7979120.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQLNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYLFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            39330..40031
                     /locus_tag="ACKB38_000274"
     CDS             39330..40031
                     /locus_tag="ACKB38_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979121.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQALTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            40033..42741
                     /locus_tag="ACKB38_000275"
     CDS             40033..42741
                     /locus_tag="ACKB38_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGL7979122.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     YKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVVAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42837..43328)
                     /gene="tpx"
                     /locus_tag="ACKB38_000276"
     CDS             complement(42837..43328)
                     /gene="tpx"
                     /locus_tag="ACKB38_000276"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGL7979123.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43451..44380)
                     /gene="psaA"
                     /locus_tag="ACKB38_000277"
     CDS             complement(43451..44380)
                     /gene="psaA"
                     /locus_tag="ACKB38_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGL7979124.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEANLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(44412..45260)
                     /locus_tag="ACKB38_000278"
     CDS             complement(44412..45260)
                     /locus_tag="ACKB38_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGL7979125.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(45257..45979)
                     /locus_tag="ACKB38_000279"
     CDS             complement(45257..45979)
                     /locus_tag="ACKB38_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGL7979126.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            46270..48162
                     /gene="pepO"
                     /locus_tag="ACKB38_000280"
     CDS             46270..48162
                     /gene="pepO"
                     /locus_tag="ACKB38_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGL7979127.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(48285..48914)
                     /locus_tag="ACKB38_000281"
     CDS             complement(48285..48914)
                     /locus_tag="ACKB38_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGL7979128.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            49873..52095
                     /locus_tag="ACKB38_000282"
     CDS             49873..52095
                     /locus_tag="ACKB38_000282"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HGL7979129.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            52125..52568
                     /gene="dtd"
                     /locus_tag="ACKB38_000283"
     CDS             52125..52568
                     /gene="dtd"
                     /locus_tag="ACKB38_000283"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGL7979130.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(52607..>52790)
                     /locus_tag="ACKB38_000284"
     CDS             complement(52607..>52790)
                     /locus_tag="ACKB38_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonyl-tRNA synthetase"
                     /protein_id="HGL7979131.1"
                     /translation="ILLWDSLMILSLVSLSDIPLFLQGTLLILGHLIPSYRICQSLKR
                     DFPQAYQEPISFWSIL"
BASE COUNT        16064 a         9488 c        11670 g        15568 t
ORIGIN      
        1 taaaaataga aggagatagc aggttttcaa gcctgctatc tttttttgat gacattcagg
       61 ctgatacgaa atcataagag gtctgaaact actttcagag tagtctgttc tataaaatat
      121 agtagattga aataagatgt gaacaactct atcaggaaag tcaaattaat ttatagaatt
      181 attttagcag tcaaggtgta ctgttataga ttcaatatat tatatgacta ttaaccttgt
      241 cttctcctaa aattgacttt cttgttttct tatcttgtcc actcgaaaca agtattgtaa
      301 gaatttgatt atttttgaaa gtacttttaa tatacttgat atagttaaaa aagatttgaa
      361 actaaattcc aaattagaaa aagacttgaa atactaaaaa aaaaaagtat actctaattg
      421 aaaacggtaa caaaactaat ttagagaatg aaatatagag tatttctctc ttaaaagttt
      481 ttggtgaaac gagatgtaga aaggagattt agccaaagag tctattagtg ctagaataat
      541 agattagaat tattttagaa aaacgaagtg agcagcttat aaattcaagt ccccaaatag
      601 attcatacta gtatcttttg caaaaaataa agggcgactt ccttcatgaa tatcaatttc
      661 atctataagg aaggtagcta attgaactaa cttatttatt ctgtttgtcg ctagaaaaat
      721 cagacctcct tgtgaagatt gaggagatac ttaatgaaaa tcaaagaaga aactagcaag
      781 ctagtagcag attgcccaaa acaccgcttt gaggttgtag ataagactga cctatataat
      841 ccaaggtgaa gcgactgtgg tttgaagaga ttttcaaaga gtataggcta gagagtagtg
      901 tttttatgtc cttctagtag aaaatgctag acagaagaat ggggaacttg gataggaaaa
      961 atagattgag aaaggaggtt agaagagatg attattacaa aaattagccg tttaggaact
     1021 tatgtgggag taaatccaca ttttgcaaca ttaatagatt ttctagaaaa aacaggacta
     1081 gaaaatttaa cagaaggttc gattgctatc gatggtaatc gattgtttgg gaattgcttt
     1141 acttatctag cagatggtca agcaggggct ttctttgaaa cccaccaaaa atatttggat
     1201 attcatttag ttttggaaaa cgaagaagcc atggctgtta catcgccgga aaatgtaagc
     1261 gttacccaag aatatgatga agagaaagat attgaattat acacagggaa agtggaacag
     1321 ttggttcatt tgagagctgg cgaatgcctc atcacttttc cagaagattt acatcaaccc
     1381 aaggttcgta taaatgatga acctgtgaaa aaagttgtct ttaaagttgc gatttcttaa
     1441 tgtagaaaga gaagaacgat gaaaaaaatg agaaagtttt tatgtctagc tggaattgcg
     1501 ctagcggctg ttgccttggt agcttgttca ggaaaaaaag aagctacaac tagtactgaa
     1561 ccaccaacag aattatctgg tgagattaca atgtggcact cctttactca aggaccccgt
     1621 ttagaaagta ttcaaaaatc agcagatgct ttcatgcaaa agcatccaaa aacgaaaatc
     1681 aagattgaaa cattttcttg gaatgacttc tatactaaat ggactacagg tttagcaaat
     1741 ggaaatgtgc cagatatcag tacagctctt cctaaccaag taatggaaat ggtcaactca
     1801 gatgctttgg ttccgctaaa tgattctatc aagcgtattg gacaagataa atttaacgaa
     1861 actgccttaa atgaagcaaa aatcggagat gattactact ctgttcctct ttattcacat
     1921 gcacaagtca tgtgggttag aacagatttg ttaaaagaac ataatattga ggttcctaaa
     1981 acttgggatc aactctatga agcttctaaa aaattgaaag aagctggagt ttatggcttg
     2041 tctgttccgt ttggaacaaa tgacttaatg gcaacacgtt tcttgaactt ctacgtacgt
     2101 agtggtggag gaagcctctt aacaaaagat cttaaagcag acttgacaag ccaacttgct
     2161 caagatggta ttaaatactg ggttaaattg tataaagaaa tctcacctca agattctttg
     2221 aactttaatg tccttcaaca agctaccttg ttctatcaag gaaaaacagc atttgacttt
     2281 aactctggct tccatatcgg aggaattaat gccaacagtc ctcaattgat tgattcgatt
     2341 gatgcttatc ctattccaaa aatcaaagag tctgataaag accaaggaat tgaaacctca
     2401 aacattccaa tggttgtttg gaaaaattca aaacatccag aagttgctaa agcattctta
     2461 gaagcacttt ataatgaaga agactacgtt aaattccttg attcaactcc agtaggtatg
     2521 ttgccaacta ttaaggggat tagcgattct gcagcctata aagaaaatga aactcgtaag
     2581 aaatttaaac atgctgaaga agtaattact gaagctgtta aaaaaggtac tgctattggt
     2641 tatgaaaatg ggccaagtgt acaagctggt atgttgacta accaacacat tattgaacaa
     2701 atgttccaag atatcattac aaatggaaca gatcctatga aagcagcaaa agaagcagaa
     2761 aaacaattaa atgatttatt tgaggctgtt cagtagatgt aaaagactag aaaataggtg
     2821 ggatagtgag ctgaaaagct ctagcccaat cttgtaaaag aagggagaag gagaatggtt
     2881 aaagaacgta atttaactcg ctggatattt gttttgccag ctatgattat cgtaggatta
     2941 ctctttgttt atccgttttt ctcgagtatt ttttatagct ttaccaataa gcatttgatt
     3001 atgcctaatt ataaatttgt tggtttggct aactataaag ctgtgctatc agatcccaac
     3061 ttctttaatg cgttctttaa ttcaattaag tggaccgttt tctcattagt tggtcaagtt
     3121 ttagtagggt ttgtattggc tttagctctt cacagagtac gccacttcaa gaaattatat
     3181 aggacattat tgattgttcc ttgggcattt cctaccatcg ttattgcctt ctcttggcag
     3241 tggattctaa acggggttta tggctactta cctaatctaa tcgtaaaatt aggtttaatg
     3301 gaacatacac ctgcattttt gacagatagt acatgggcat tcctatgttt ggtgtttatc
     3361 aacatttggt ttggagcacc aatgattatg gttaatgtgc tttcagcttt gcaaacagta
     3421 ccagaagaac aatttgaggc tgctaagata gatggtgctt caagttggca ggtgttcaag
     3481 tttatcgtct ttccacatat taaagtggtt gtaggacttc tagttgtttt gagaactgta
     3541 tggatcttta ataactttga cattatctac ctcattactg gtggtggacc agccaatgct
     3601 acaacgacgc ttccaatttt tgcttacaac ctgggctggg gaactaaatt gttgggtcgt
     3661 gcttcagcag ttacagtact gctctttatc ttcttggtgg cgatttgctt tatctacttt
     3721 gctatcatca gtaagtggga aaaggagggt agaaaataat gaagaagaaa tccagtattt
     3781 atttagatat tctctcacat gtacttttag ttggtgcgac catcgttgca gttttcccat
     3841 tggtatggat tatcatatct tctgtcaaag ggaaagggga attaactcag tatccaacac
     3901 gattttggcc tgaacagttt acattagatt atttcactca tgttatcaac gatttgcact
     3961 tcattgataa cattcgaaac agtttaatca ttgccttggc tacaaccctt attgcgatta
     4021 ttatttctgc tatggcagcc tatggtattg ttcgattctt tcctaaattg ggagcaatca
     4081 tgtcgagact actcgtcatt acctacattt tcccaccaat tttgttagca attccctatt
     4141 caattgccat tgctaaagtt gggttaacaa atagtttatt tggcttgatg atggtttatc
     4201 tatcttttag tgttccatat gcagtttggc tcttagttgg atttttccaa acagttccaa
     4261 ttggaattga agaagcggct agaattgatg gtgcaaataa atttgttacg ttttataaag
     4321 ttgtgctacc gattgtagca ccaggtattg tagcaacagc tatttataca tttatcaatg
     4381 cttggaatga attcctgtat gccttgattt tgattaacaa tacaggaaag atgacagtag
     4441 cagtagccct tcgttcactt aatggttcag aaatactaga ctggggagat atgatggcag
     4501 cgtctgttat tgtagttctt ccatcaatta ttttcttctc tatcatccaa aataagattg
     4561 caagtggatt atcagaagga tctgtgaagt agacgaaaga aggaaaaaaa tgaataaaag
     4621 aggtctttat tcaaaactag gaatttccgt tgtaggcatt agtcttttaa tgggagtccc
     4681 cactttgatt catgcgaatg aattaaacta tggtcaactg tccatatctc ctatttttca
     4741 aggaggttca tatcaactga acaataagag tatagatatc agctctttgt tattagataa
     4801 attgtctgga gagagtcaga cagtagtaat gaaatttaaa gcagataaac caaactctct
     4861 tcaagctttg tttggcctat ctaatagtaa agcaggcttt aaaaataatt acttttcaat
     4921 tttcatgaga gattctggtg agataggtgt agaaataaga gacgcccaaa agggaataaa
     4981 ttatttattt tccagaccag cttcattatg gggaaaacat aaaggacagg cagttgaaaa
     5041 tacactagta tttgtatctg attctaaaga taaaacatac acaatgtatg ttaatggaat
     5101 agaagtgttc tctgaaacag ttgatacatt tttgccaatt tcaaatataa atggtataga
     5161 taaggcaaca ctaggagctg ttaatcgtga aggtaaggaa cattacctcg caaaaggaag
     5221 tattgatgaa atcagtctat ttaacaaagc aattagtgat caggaagttt caactattcc
     5281 cttgtcaaat ccatttcagt taattttcca atcaggagat tctactcaag ctaactattt
     5341 tagaataccg acactatata cattaagtag tggaagagtt ctatcaagta ttgatgcacg
     5401 ttatggtggg actcatgatt ctaaaagtaa gattaatatt gccacttctt atagtgatga
     5461 taatgggaaa acgtggagtg agccaatttt tgctatgaag tttaatgact atgaggagca
     5521 gttagtttac tggccacgag ataataaatt aaagaatagt caaattagtg gaagtgcttc
     5581 attcatagat tcatccattg ttgaagataa aaaatctggg aaaacgatat tactagctga
     5641 tgttatgcct gcgggtattg gaaataataa tgcaaataaa gccgactcag gttttaaaga
     5701 aataaatggt cattattatt taaaactaaa gaagaatgga gataacgatt tccgttatac
     5761 agttagagaa aatggtgtcg tttataatga aacaactaat aaacctacaa attatactat
     5821 aaatgataag tatgaagttt tggagggagg aaagtcttta acagtcgaac aatattcggt
     5881 tgattttgat agtggctctt taagagaaag gcataatgga aaacaggttc ctatgaatgt
     5941 tttctacaaa gattcgttat ttaaagtgac tcctactaat tatatagcaa tgacaactag
     6001 tcagaataga ggagagagtt gggaacaatt taagttgttg cctccgttct taggagaaaa
     6061 acataatgga acttacttat gtcccggaca aggtttagca ttaaaatcaa gtaacagatt
     6121 gatttttgca acatatacta gtggagaact aacctatctc atttctgatg atagtggtca
     6181 aacatggaag aaatcctcag cttcaattcc gtttaaaaat gcaacagcag aagcacaaat
     6241 ggttgaactg agagatggtg tgattagaac attctttaga accactacag gtaagatagc
     6301 ttatatgact agtagagatt ctggagaaac atggtcgaaa gtttcgtata ttgatggaat
     6361 ccaacaaact tcatatggca cacaagtatc tgcaattaaa tactctcaat taattgatgg
     6421 aaaagaagca gtcattttga gtacaccaaa ttctagaagt ggccgcaagg gaggccaatt
     6481 agttgtcggt ttagtcaata aagaagatga tagtattgat tggaaatacc actatgatat
     6541 tgatttgcct tcgtatggtt atgcctattc tgcgattaca gaattgccaa atcatcacat
     6601 aggtgtactg tttgaaaaat atgattcgtg gtcgagaaat gaattgcatt taagcaatgt
     6661 agttcagtat atagatttgg aaattaatga tttaacaaaa taaaggagaa aaacatggtt
     6721 aaatacggtg ttgttggaac agggtatttt ggagctgaat tggctcgcta catgcaaaag
     6781 aatgatagag cagagattac tcttctctat gatccagata atgcagaggc gattgcagaa
     6841 gaattgggag caaaagtagc aagttcctta gatgagttgg tttctagcga tgaagtagat
     6901 tgtgttatcg tcgcaactcc aaataatctt cataaggaac cggttattaa ggctgcacag
     6961 catggtaaaa atgttttctg tgaaaaacca attgcgcttt cttatcaaga ttgtcgcgag
     7021 atggtagatg cgtgtaaaga aaacaatgta acctttatgg caggacatat tatgaatttc
     7081 tttaatggtg ttcatcatgc aaaagaactc attaatcaag gagttatcgg agacgttcta
     7141 tattgtcata cagctcgtaa tggttgggaa gaacaacaac cgtcagtatc atggaaaaaa
     7201 attcgtgaaa aatcaggtgg tcacttgtat caccacatcc atgaattgga ttgcgttcaa
     7261 ttccttatgg ggggcatgcc tgaaactgta accatgacag gtggaaatgt ggcccatgaa
     7321 ggtgaacatt tcggtgatga agatgatatg atttttgtca atatggaatt ttctaataag
     7381 cgttttgcct tgttagaatg gggttcagct tatcgttggg gtgaacatta tgtcttaatc
     7441 caaggaagca aaggtgccat ccgcttagac ttattcaact gtaaaggaac tcttaagcta
     7501 gatgggcaag aaagctattt cttgattcac gaatcgcaag aagaagatga tgatcggact
     7561 cgtatctatc atagtacaga gatggatgga gcaattgctt atggtaaacc aggtaaacgt
     7621 actccattat ggctatcatc tgtcattgat aaagaaatgc gctatctgca tgagattatg
     7681 gaaggagctc cagtatcaga agaatttgca aaacttttga caggtgaagc tgccctagaa
     7741 gcaattgcta ctgcagatgc ttgtacccag tctatgtttg aagatcgcaa agtaaaattg
     7801 tcagaaattg taaaataaat tttggtattc tcctatttat aggtcgactt gctcctctga
     7861 aagtactttt agaggagctg tttgactttg ctagtttttg aaactgaaat ctattatact
     7921 acaaactatt gaaagcgttt taattttaag gtataataat ctcatagaaa taaagaaaag
     7981 gaggaaagag gatgccacag attagcaaag aagccttgat tgagcaaatc aaagatggaa
     8041 tcatcgtttc ttgtcaggct cttcctcatg aaccgcttta tacagaagcg ggaggggtga
     8101 ttcccttgct ggtcaaagcg gctgagcaag gtggagcagt cggtatccga gcaaacagtg
     8161 ttcgcgatat caaggaaatt aaggaagtca ctaaacttcc aatcattggg attatcaaac
     8221 gtgattatcc acctcaggaa cccttcatca cggctactat gaaagaagtt gatgaattgg
     8281 cagaactgga catcgaggtg attgctctgg attgtaccaa gcgtgaacgc tacgatggtt
     8341 tggaaattca agagttcatt cgtcaggtta aggagaaata tcctaatcag cttttgatgg
     8401 ctgatactag tatcttcgaa gaagggctag cagctgtaga agcaggaatt gactttgtcg
     8461 gaacaacctt atcaggctac acatcctaca gtccaaaagt agacggtcca gattttgaat
     8521 tgattaagaa actctgtgat gctggtgtag atgtcattgc agaaggaaaa attcatacac
     8581 cagaacaagc caaacaaatc cttgaatatg gagtgcgagg catcgttgtt ggtggcgcca
     8641 ttactagacc aaaagagatt acagaacgct tcgttgctag tcttaaataa gatgtgaggg
     8701 ggagttttat gtttaaagtt ttacaaaaag ttggaaaagc ttttatgtta cctatagcta
     8761 tacttcctgc agcaggtcta cttttgggga ttggtggtgc actttcaaac ccaaccacga
     8821 tagcaactta tccaatacta gacaatagta tttttcaatc aatattccaa gtaatgagct
     8881 ctgcaggaga ggttgtattc agtaatttgt cactacttct ctgtgtggga ttatgtattg
     8941 gcttagcgaa acgagataaa ggaaccgctg cgttagcagg agtaactggt tacttagtta
     9001 tgactgcaac gatcaaagct ttggtaaaac tttttatggc agaaggatct gcaattgata
     9061 ctggagttat tggagcatta gttgtcggaa tagttgccgt atatttgcac aaccgatata
     9121 acaatattca attaccttcc gctttaggat tctttggagg ttcacgcttc gttcctattg
     9181 ttacatcgtt ctcttctatc ttgattggct ttgtcttctt tgttatttgg ccacctttcc
     9241 aacaacttct tgtttctaca ggtggatata tttctcaggc gggtccaatt ggaacttttc
     9301 tatatggatt tttaatgaga ctttctggag cagtaggctt acatcatata atttacccta
     9361 tgttttggta tactgaactt ggtggtgttg aaactgttgc aggacaaaca gtggttggag
     9421 ctcaaaaaat attttttgct caattagccg atttggccca ttctggatta tttacagaag
     9481 gaacaaggtt ttttgcaggt cgtttctcaa caatgatgtt cggtttaccg gctgcctgtt
     9541 tagcgatgta ccatagtgtt cctaaaaatc gtcgtaaaaa atatgcgggt ttgttttttg
     9601 gagttgcttt aacatctttt attaccggta ttacagaacc aattgaattt atgtttctat
     9661 tcgtcagtcc ggttctatat gttgttcacg cattccttga tggtgttagc ttctttattg
     9721 cagacgtctt aaatatttca ataggaaaca cattttcagg aggtgtaatc gatttcactt
     9781 tatttggaat tttgcagggg aacgctaaga cgaattgggt tcttcagatt ccatttggac
     9841 ttatttggag tgttttgtat tatattattt ttagatggtt cattactcaa ttcaacgttc
     9901 taacgccagg gcgaggagaa gaagtagatt ctaaagaaat ttctgaatcc gcagattcaa
     9961 cttcaaatac tgcagattat ttaaaacagg atagcctaca aattatcaga gccttgggtg
    10021 gatcaaataa tatagaagat gtagatgctt gtgtgacacg tttacgtgta gctgtaaaag
    10081 aagttaatca agttgataaa gcacttttaa aacaaattgg tgcagttgat gtcttagaag
    10141 tgaagggtgg cattcaagca atctatggag caaaagcaat cttatataaa aatagtatta
    10201 atgaaatttt aggtgtagat gattaagtac ttactgactt aataaaaaac agaggagagt
    10261 gatggatgag taggatgaaa tgaaatcgca tacaagaaat aaagaactca ttatccaagt
    10321 tggatacgct tattacatag gagaatacaa atgaaattta gaaaattagc ttgtacagta
    10381 cttgcgggtg ctgcggttct tggtcttgct gcttgtggca attctggcgg aagtaaagat
    10441 gctgccaaat caggtggtga cggtgccaaa acagaaatca cttggtgggc attcccagta
    10501 tttacccaag aaaaaactgg tgacggtgtt ggaacttatg aaaaatcaat catcgaagcg
    10561 tttgaaaaag caaacccaga tataaaagtg aaattggaaa ccatcgactt caagtcaggt
    10621 cctgaaaaaa tcacaacagc catcgaagca ggaacagctc cagacgtact ctttgatgca
    10681 ccaggacgta tcatccaata cggtaaaaac ggtaaattgg ctgagttgaa tgacctcttc
    10741 acagatgaat ttgttaaaga tgtcaacaat gaaaacatcg tacaagcaag taaagctgga
    10801 gacaaggctt atatgtatcc gattagttct gccccattct acatggcaat gaacaagaaa
    10861 atgttagaag atgctggagt agcaaacctt gtaaaagaag gttggacaac tgatgatttt
    10921 gaaaaagtat tgaaagcact taaagacaag ggttacacac caggttcatt gttcagttct
    10981 ggtcaagggg gagaccaagg aacacgtgcc tttatctcta acctttatag cggttctgta
    11041 acagatgaaa aagttagcaa atatacaact gatgatccta aattcgtcaa aggtcttgaa
    11101 aaagcaacta gctggattaa agacaatttg atcaataatg gttcacaatt tgacggtggg
    11161 gcagatatcc aaaactttgc caacggtcaa acatcttaca caatcctttg ggcaccagct
    11221 caaaatggta tccaagctaa acttttagaa gcaagtaagg tagaagtggt agaagtacca
    11281 ttcccatcag acgaaggtaa gccagctctt gagtaccttg taaacgggtt tgcagtattc
    11341 aacaataaag acgacaagaa agtcgctgca tctaagaaat tcatccagtt tatcgcagat
    11401 gacaaggagt ggggacctaa agacgtagtt cgtacaggtg ctttcccagt ccgtacttca
    11461 tttggaaaac tttatgaaga caaacgcatg gaaacaatca gcggctggac tcaatactac
    11521 tcaccatact acaacactat tgatggattt gctgaaatga gaacactttg gttcccaatg
    11581 ttgcaatctg tatcaaatgg tgacgaaaaa ccagcagatg ctttgaaagc cttcactgaa
    11641 aaagcgaacg aaacaatcaa aaaagctatg aaacaatagt ccttagttat tctataaaaa
    11701 gtagtttttt aaagaaccta agagtgtata cccccttttc cctctacaca gatagtgtaa
    11761 gaaaaggggg cttttgttta aaatgtaaga aactgtcacg aaattaaaat gaagttctta
    11821 cataagcgaa tcataaaaaa tttcattttg attttaaaac agttcaagaa agtcaaaaaa
    11881 ttattctatt tgaaagagag gtgccgactg tgaaagtcaa taaaatccgt atgcgggaaa
    11941 cagtgatttc ctacgctttc ctagcaccag tattattctt ctttgtcatc tttgtgttgg
    12001 ctccgatggt gatgggcttc attacaagtt tctttaacta ctcaatgact aaatttgagt
    12061 ttgtaggctt ggataactat atccgtatgt ttaaagatcc tgtctttaca aaatctctga
    12121 ttaacacagt tattttggtt attggatctg taccagttgt tgttctattc tcactctttg
    12181 tagcatctca gacctatcat caaaatgtca ttgccagatc cttctaccgt ttcgtcttct
    12241 tccttcctgt tgtaacgggt agtgttgccg tgacagttgt ttggaaatgg atttatgacc
    12301 cactatcagg gattctaaac tttgtcctta agtccagcca catcatcagc caaaacattt
    12361 cttggttggg agataaaaac tgggcattga tggcgattat gattattctc ttgaccactt
    12421 cagttggtca gcccatcatc ctttatatcg ctgccatggg gaatattgac aattcactgg
    12481 ttgaagcggc gcgtgttgat ggtgcaactg agtttcaagt tttttggaag attaaatggc
    12541 caagccttct tccaacaact ctttatattg caatcatcac aacaattaac tcattccagt
    12601 gtttcgcctt gattcagctt ttgacatctg gtggtccaaa ctactcaaca agtaccttga
    12661 tgtactacct ttacgaaaaa gccttccaat tgacagaata cggctatgcc aacacaattg
    12721 gtgtcttctt ggcagtcatg attgctatcg taagctttgt tcaatttaaa gtacttggaa
    12781 acgacgtaga atactaaaga aaggagacag ctatgcaatc tacagaaaaa aaaccattaa
    12841 cagcctttac tgttatttca acaatcattt tgctcttgtt gactgtgctg ttcatctttc
    12901 cattctactg gattttgaca ggggcattca aatcacaacc tgatacaatt gttattcctc
    12961 ctcagtggtt ccctaaaatg ccaaccatgg aaaacttcca acaactcatg gtgcagaacc
    13021 ctgccttgca atggatgtgg aactcagtat ttatctcatt ggtaaccatg ttcttagttt
    13081 gtgcaacctc atctctagca ggttatgtat tggctaaaaa acgtttctat ggtcaacgca
    13141 ttctatttgc tatctttatc gctgctatgg cgcttccaaa acaagttgtc cttgtaccat
    13201 tggtacgtat cgtcaacttc atgggaatcc atgatactct ctgggcagtt atcttgcctt
    13261 tgattggatg gccattcggt gtcttcctca tgaaacagtt cagtgaaaat atccctacag
    13321 agttgcttga atcagctaaa atcgacggtt gtggtgagat tcgtaccttc tggagtgtag
    13381 ccttcccgat tgtgaaacca gggtttgcag cccttgcaat ctttaccttc atcaatactt
    13441 ggaatgacta cttcatgcaa ttggtaatgt tgacttcacg taacaatttg accatctcac
    13501 ttggggttgc gaccatgcag gctgaaatgg caaccaacta tggtttgatt atggcaggag
    13561 ctgcccttgc tgctgttcca atcgtcacag tcttcctagt cttccaaaaa tccttcacac
    13621 agggtattac tatgggagcg gtcaaaggat aatactctgc gaaaatctct tcaaactacg
    13681 tcagcttcac cttgccatac ttaagtattg cctgcggtta gcttcctagt ttgttcttca
    13741 attttcattg agtataggaa aatcaatcta tcaagataca gaagtatatt ttatagattt
    13801 agagaatata gaggttataa gtgtctacaa aatggagggt atgcagttac tttatgaagt
    13861 tttgtcagac acttataaac ttaagaatgg ttttagttaa ctatcagaaa cgaaggaaag
    13921 agtatgattt ttgacgattt gaaaaacatc accttttaca aagggattca tcctaattta
    13981 gacaaggcta tcgactatct ctaccaacat cgtaaggatt ctttcgaatt aggaaagtat
    14041 gatattgatg gagataaagt ctttctagtt gttcaggaaa atgtcctcaa tcaagctgaa
    14101 aatgatcaat ttgagtatca taagaactat gcagatttgc atttgctggt agaaggacat
    14161 gaatattcga gctacggttc acgtatcaaa gacgaggcag tagcattcga cgaagcgagt
    14221 gacattggct ttgttcattg tcatgaacac tacccactct tgttgggtta tcacaatttt
    14281 gcgattttct tcccaggtga gccacatcag ccaaatggtt atgcaggcat ggaagaaaag
    14341 gttcgaaaat atctctttaa aattttgatt gattaaaaat aggatgaatt gtttttttgt
    14401 aaagctttga taatactcta ccatgaaatt gatctttgtg aggtagagaa atgagaataa
    14461 aatatttaaa aattggtatc ttctaagtat gctgcaagag ctagtttctt agatggacag
    14521 gggattacag ttgatgagat ggcttggata attaggggca ttgtgaatgc attgattggt
    14581 agatacataa aattaggtac ttatgcggct aagtatggta ttagtatggc acgctcgatc
    14641 ttaagtaggg tagctgcaac tgcagcagca agagtaggat tactgaccaa gatttctgga
    14701 tggattttac gagtagctgt gaatgtagct gatgtatatg gtaattttgc caacaatatt
    14761 gctgcagctt gggatgcata tgataaaatt cctaacaatg gtcgtataaa cttttaaaat
    14821 gcgagaatga aagcactttg tattttttta ttgaatatgt tagcttggac agtgcttgca
    14881 atgataattc gtggagggct agatggattt gataggcata cttggagtac tattttaatt
    14941 gcttcgctgt tcggggtata tgattataag cccatagata aaaatagaaa aaagtccaaa
    15001 agaaaaaata gatttgttca tggtagggac ttatgaaagc tttactgaca aaaaagaaaa
    15061 cagtttacaa agaaaaatga tggaggagca aacatggcac aaaaaggagt aagccttatc
    15121 aaggcagcat ttgatacaga taactttctc atgcgtttta gtgagaaggt cttggacatc
    15181 gtgacagcca atcttctttt tgtcgtctct tgtttaccca tcgtgacgat tggagtggct
    15241 aaaatcagcc tctacgagac catgttcgaa gttaagaaga gcagacgggt gcctgttttt
    15301 aaaatctatc taagatcttt caagcaaaat ctgaaactag gtcttcagct gggtttaatg
    15361 gagttaggaa ttgtgtttct taccctttca gatctctatc ttttctgggg tcaaacagct
    15421 ctgcccttcc aattgctgaa agccatttgt ttaggtattc tgatttttct tactatcgtg
    15481 atgctggcta gttaccctat cgcggcacgt tatgacctat cttggaaaga aattcttcaa
    15541 aaaggattga tgttggctag ttttaacttt ccttggttct tcctcatgtt agccattctt
    15601 gtcctcattg tgatggttct ttatctgtcc gccttcagtc tactcttagg tggctcagtc
    15661 ttcctacttt ttgggtttgg actattggtc tttatccaga ctggattgat ggagaaaatt
    15721 ttcgcaaaat accaatagga gctttatttc tgaaactact ttcaaaggct ccaaacgcta
    15781 ttctataagc gagaaactaa aatctaagtg tctataagat attgaaagcc ttttcggtaa
    15841 agtgtatact aaagaagtaa agaaaaatct gctttagcag aaaataaaaa taataggaga
    15901 aaatctatgt cagatttgaa aaaatacgaa ggtgtcattc cagccttcta cgcatgttat
    15961 gatgatcaag gagaagtaag cccagaacgt acgcgtgcct tggttcaata cttcattgat
    16021 aaaggtgttc aaggtcttta tgtcaatggt tcttctggtg aatgtatcta ccaaagcgtt
    16081 gaagatcgca agttgatttt ggaagaagtc atggcggtag ccaaaggtaa attgaccatt
    16141 attgcccatg ttgcttgcaa taatactaaa gatagtatgg aacttgctcg ccatgctgaa
    16201 agcttgggag tagatgctat tgcaacgatt ccaccaattt atttccgctt gccagaatac
    16261 tcagttgcca aatactggaa cgatatcagt tctgcagctc caaacacaga ctacgtgatt
    16321 tacaacattc ctcaattggc aggggttgct ttgactccaa gcctttacac agaaatgttg
    16381 aaaaatcctc gtgttatcgg tgtgaagaac tcttctatgc cagttcaaga tatccaaacc
    16441 tttgtcagcc ttggtggaga agaccatatc gtctttaatg gtcctgatga gcagttccta
    16501 ggaggacgcc tcatgggggc tagggctggt atcggtggta cttatggtgc tatgccagaa
    16561 ctcttcttga aactcaatca gttgattgcg gataaggacc tagaaacagc gcgtgaattg
    16621 cagtatgcta tcaacgcaat cattggtaaa ctcacttctg ctcatggaaa tatgtacggt
    16681 gtcatcaaag aagtcttgaa aatcaatgaa ggcttgaata ttggatctgt tcgttcacca
    16741 ttgacaccag tgactgaaga agatcgtcca gttgtagaag cggctgctgc cttgattcgt
    16801 gaaaccaagg agcgcttcct ctaatctaaa aggaggtatt tatgacatat tacgttgcaa
    16861 ttgatatcgg tggaaccaac atcaagtatg gtttggttga tcaagagggg caacttcttg
    16921 aatcgcatga aatgccaact gaggcgcata agggtggacc tcatatctta caaaagacca
    16981 aagatatcgt agctagttat ttagaaaaag gcccagtagc aggtgttgcc atatcttctg
    17041 ctgggatggt ggatccggat aagggtgaga ttttctatgc tgggccgcaa atccctaact
    17101 acgcaggcac ccagttcaaa aaggaaatcg aagaaagctt tactattcct tgtgagattg
    17161 aaaatgatgt caactgtgca ggtcttgctg aggcagtatc tggttcaggc aagggagcaa
    17221 gtgtgacact ttgcttgacc attggaaccg gtatcggtgg ttgcttgatt atggatagga
    17281 aagtcttcca tggttttagc aattcagcct gtgaagtcgg gtatatgcat atgcaggatg
    17341 gagcttttca agacttggct tctacaacag ctttagtgaa atatgtagct gaagcccatg
    17401 gagaagatgt tgatcagtgg aatggccgta gaattttcaa agaagccact gaaggaaaca
    17461 aaatctgcat ggaaggtatt gaccgtatgg ttgactatct aggaaaaggt ctggcaaata
    17521 tttgctacgt tgccaatcca gaagtggtta ttcttggtgg tggtatcatg gggcaagagg
    17581 ctatcctcaa acctaagatc cgtacagcct tgaaagaggc tttggtacca agtttagcag
    17641 aaaaaacacg attagaattt gcccatcacc aaaatacagc agggatgttg ggtgcatatt
    17701 atcattttaa gacaaaacaa tcctagtttg gctcagccaa actaggattt tcttacacgt
    17761 ttttgtctac gatagccgtt gagtttttta ttttcccagt agctattaaa gattttttcc
    17821 ttgctttcgc gattgatttc caaaaagtag gcataaatca aatcgataaa gaagagcata
    17881 ggaagttgag cggatattcg ttggatatag gagggttggc tgtgggtggc tacaagaaca
    17941 gtctctgtat aggtctggct atctttattg ggaacacttg taaagagtac agtctttgcc
    18001 cccatctcct tagcatctaa tagactatct aaaatagaag gagttgagcc tgaaagtgag
    18061 aagcccagta ctagacaatt ttcatccatg atgctggttg tccaggcaaa gccgtcttgg
    18121 tctgtcaaag cttcgcagac cacacctagt cgcataaaac gtaatttcat ttcacgggcg
    18181 acgaggccag aactccctgt tccaaagaag tagatacgct cagcatcttc gattagctgg
    18241 gcaattcgtt ctagttggat ttcgtcaatc aagtcttgtg tttgttccct catattgcta
    18301 taacttctga ggactcgttt ggtcagtgga ctgtgcttgg agacttggtt ggcttgattt
    18361 tctgcctgat gttggtattg gaaaataaat tctcggtagc cagtaaagcc acacttttta
    18421 gcaaagcggg tcaaagcagc ttgagaaata tgtaattttt gggtgacttg ttgagaagat
    18481 aaatcatctg taatcgtttc agcttgcaaa aaatagcgag cgatttcttg ttctaggtct
    18541 gtcatttctt caaaatgtga atcaatgata gttgcgatat ctggtttgtc catagggaaa
    18601 gctcctttac atgagtcata ctggaagact agatcagaga atagtcacac ttcattataa
    18661 cacataatat aaggatagat aaataaaaac gcatctctgt tttaaaaacg aaaaaatcga
    18721 aaaagcttct ctcttttcca taattttcta ctcaaattgt ggtacaatta agagtaagat
    18781 tttaagttag aaatgagact gatttgtatg agaaaattta acagccattc gattccgatt
    18841 cggcttaatt tattgttttc aatcgtcatt ttactcttta tgaccattat tggtcgtttg
    18901 ttgtatatgc aggttttgaa caaggatttt tacgaaaaaa agctagcttc agctagtcag
    18961 accaagatta caagcagttc agcccgtggg gaaatttatg atgctagtgg aaaacctttg
    19021 gtagaaaata cgttaaagca ggttgtttcc tttacgcgta gcaataaaat gacggctaca
    19081 gacttaaaag aaacagctaa aaagttactg acttatgtga gcatcagttc tccaaatttg
    19141 acagaacgcc agctggcgga ttactatttg gctgatcctg aaatctataa aaaaatagtg
    19201 gaagctctcc caagtgagaa acgcttggat tcagatggca atcgtctatc cgaatcagaa
    19261 ctgtataaca atgcggtcga tagtgtacaa acgagtcaac taaactatac agaggatgaa
    19321 aagaaagaaa tctatctttt tagtcagtta aatgctgttg gaaactttgc gacaggaacc
    19381 attgcgacag atcctctaaa tgattctcag gtggctgtta ttgcctctat ttcaaaggag
    19441 atgcctggca ttagtatttc tacttcttgg gatagaaagg ttttggaaac ttccctttct
    19501 tctatagttg ggagtgtatc cagtgaaaaa gctggtctcc cagcggaaga agcagaagcc
    19561 tatcttaaaa aaggctattc tctaaatgac cgtgtaggaa cctcctattt ggaaaagcaa
    19621 tatgaagaga ccttacaagg aaaacgctcg gtaaaagaaa tccatctgga taaatatggc
    19681 aatatggaaa gcgtggatac aattgaggaa ggtagtaagg gaaacaatat caaactgacc
    19741 attgatttgg ctttccaaga tagcgtggat gctttactga aaagttattt caattctgag
    19801 ctagaaaatg gtggagccaa gtattctgaa ggtgtctatg cagtcgccct taacccaaaa
    19861 acaggtgcgg ttttgtctat gtcagggatt aaacatgact tgaaaacggg agagttgacg
    19921 cctgattcct tgggaacggt aaccaatgtc tttgttccag gttcggttgt caaggcggcg
    19981 accatcagct caggttggga aaatggagtc ttgtcaggaa accagacctt gacagaccag
    20041 tccattgtct tccaaggttc agctcccatc aattcttggt atactcaggc ttacggttca
    20101 ttccctatca cagcggtcca agctctggag tattcatcaa atacctatat ggtccaaaca
    20161 gccttaggtc ttatggggca aacctatcaa cccaatatgt ttgtcggcac cagcaatcta
    20221 gagtctgcta tggagaaact gcgttcaacc tttggcgaat atggcttggg tactgcgaca
    20281 ggaattgacc taccagatga atctactgga tttgttccca aagagtatag ctttgctaat
    20341 tacattacta atgcctttgg gcagtttgat aactatacgc cgatgcagtt ggctcagtat
    20401 gtagcaacta ttgcaaataa tggtgttcgt gtggctcctc gtattgttga aggcatttat
    20461 ggtaataatg ataagggagg actgggtgac ttgattcagc aactgcaacc gacagagatg
    20521 aataaggtca atatatccga ctccgatatg agcatcttgc accaaggttt ttatcaggtt
    20581 gcccatggta ctagtggatt gacaactgga cgtgcctttt caaatggtgc cttggtatcc
    20641 attagcggaa aaacaggtac agccgaaagc tatgtggcag atggtcagca agcaaccaat
    20701 accaatgcgg tggcctatgc cccatctgat aatccccaaa tcgctgtcgc agtggtcttt
    20761 cctcataata ccaatctaac aaatggtgta ggaccttcca ttgcgcgtga cattatcaat
    20821 ctgtatcaaa aataccatcc aatgaattag aaaggaaatt atgctttatc caacacctat
    20881 tgccaagttg attgacagtt attctaagtt accaggtatc gggattaaga cggctacgcg
    20941 tctggccttt tatacgattg ggatgtctgc tgatgatgtc aatgaatttg caaaaaatct
    21001 cctttctgct aagagagaat tgacatattg ttctatttgt ggacgtttga cagacgacga
    21061 tccttgttct atctgtactg atccgactcg tgaccagaca acaattttag ttcttgagga
    21121 tagtagagat gtggcagcca tggaaaatat ccaagaatac catggactct atcatgtcct
    21181 tcatggcctc atttctccta tgaatggtat cagtccggac gatatcaatc tcaagagcct
    21241 tatgactcgt cttatggata gtgaggtttc agaagtgatt gtggcgacta atgctacagc
    21301 ggatggtgaa gcgacttcca tgtatctttc acgtttgctc aagccggctg gtatcaaggt
    21361 tacgcgtcta gcacgaggtc tcgctgtggg agcggacatt gagtatgcgg acgaagtgac
    21421 actcttacga gccattgaaa atcggacaga gttgtaagtg taggcaaatt tacgaactcc
    21481 attcatttat aaaaaatcaa agaggctgaa aatcgttcct atcggcctct ttttgtatag
    21541 tgtgatgagt aggctcaggt tcaagtttta aaaaaccaag caaatatgat atactaaaga
    21601 gcgagtattc tagtagaatt aggacaaata atatgaaaca aacgattatt cttttatatg
    21661 gtggacggag tgcggaacgc gaagtctctg tcctttcagc tgagagtgtc atgcgtgcgg
    21721 tcgattacga ccgtttcaca gtcaagactt tctttatcag tcagtcaggt gactttatca
    21781 aaacacagga atttagtcat gctccggggc aagaagaccg tctcatgacc aatgaaacca
    21841 ttgattggga taagaaagtt gcaccaagtg ctatctacga agaaggtgca gtggtctttc
    21901 cagtccttca cgggccaatg ggagaagatg gctctgttca aggattcttg gaagttttga
    21961 aaatgcctta cgttggttgc aacattttgt catcaagtct tgccatggat aaaatcacga
    22021 ctaagcgtgt tctggaatct gctggtattg cccaagttcc ttatgtggct atcgttgaag
    22081 gcgatgatgt gactgctaaa atcgctgaag tggaagaaaa attggcttat ccagtcttca
    22141 ctaagccgtc aaacatgggg tctagtgtcg gtatttctaa gtctgaaaac caagaagaac
    22201 tccgtcaagc cttaaaactt gccttccgat atgacagccg tgtcttggtt gagcaaggag
    22261 tgaatgcccg tgaaattgag gttggcctct tgggtaacta cgatgtcaag agcacgctac
    22321 caggagaagt tgtcaaggac gttgcctttt atgactacga tgccaagtat attgataaca
    22381 atattactat ggatattcct gccaaaatca gtgatgatgt ggtggctgtc atgcgtcaaa
    22441 atgcagaaac agccttccgt gccattggtg gccttggtct atctcgttgc gatttcttct
    22501 atacagataa gggagagatt tttctcaacg agctcaatac tatgccaggt ttcacccagt
    22561 ggtctatgta cccactactt tgggacaata tggggatcag ctacccaaaa ctaatcgagc
    22621 gtttggttga ccttgccaag gaaagttttg acaagcgcga agcgcatttg atataaaatg
    22681 aaagagaggg tagaagccag aaccatcact gcacggtgac tagagttctc ggacttcagc
    22741 cctttttaaa ggagtagaaa tgaaattaac aatccatgaa attgcccaag ttgttggagc
    22801 caaaaatgat atcagtatct ttgaggacac ccagttagaa aaagctgagt ttgatagtcg
    22861 tttgattgga actggagatt tatttgtgcc acttaaaggt gcgcgtgatg gccatgactt
    22921 tattgaaaca gcctttgaaa atggtgcagc agtaaccttg tctgagaaag aggtctcaaa
    22981 tcatccttac attctagtag atgatgtttt gacagccttt caatccttag catcctacta
    23041 tcttgaaaaa acgactgttg atgtctttgc tgttacaggt tcaaatggca agacaacgac
    23101 taaggatatg ttggcgcatt tactgtcaac aagatacaag acctacaaaa cacaaggcaa
    23161 ttacaataat gagattggcc ttccttacac agttcttcat atgcctgaag gaacagaaaa
    23221 gttggttttg gagatgggac aggatcactt gggcgatatt catctcttgt ctgaattggc
    23281 tcgtccaaaa acagccatcg tgaccttggt tggagaagcc catttggcct ttttcaaaga
    23341 ccgttcagag attgctaagg gaaaaatgca aattgcagac ggaatggctt caggttcctt
    23401 gcttttagcg ccggctgacc ctatcgtaga ggactatttg ccaactgata aaaaggtggt
    23461 tcgttttggg caaggggcag agctggaaat tactgacttg gttgagcgca aagatagtct
    23521 gaccttcaag gccaatttct tagagcaagc ccttgatttg ccagtaactg gcaagtacaa
    23581 tgcgacaaat gctatgattg catcctatgt tgccttgcaa gaaggagttt cagaggagca
    23641 aattcgtttg gccttccaag atcttgaatt gacgcgtaac cgtaccgagt ggaagaaagc
    23701 agccaatgga gcagatatcc tgtcagatgt ttacaatgcc aatccaactg ctatgaaact
    23761 gattttagag actttctctg ccattccagc caatgaaggt ggcaagaaaa ttgcagtgtt
    23821 ggcggatatg aaggagcttg gtgaccagtc tgttcaactt cataatcaga tgattttgag
    23881 cctttctcca gatgtgcttg ataccgtgat tttctatgga gaaaatattg ctgaattagc
    23941 ccaattggcc agtcaaatgt tcccaatcgg ccacgtttac tacttcaaga aaacagaaga
    24001 ccaggatcaa tttgaagacc tagtcaagca ggtcaaggaa agccttggag cccatgacca
    24061 aatcctgctc aaaggctcta actctatgaa tctagccaag ttggtagaaa gtttagaaaa
    24121 tgaagacaag tgattttgtc aagtatttgc aaagaatgat tgccattaca gatactggct
    24181 taacctttac aaaagatccg tttgaccgtg agcgctacga agacttgcga agtctgttat
    24241 ctgaaatgtt gaatcaagca tcagaccttg attccgaaga agtggcagaa gtcttgaagc
    24301 caacttctgc ttatgcgact ccgttaatgg acgtccgtgc ttggattgtt gaggatgaga
    24361 agatttgtct ggttagggga caaggagagg atagttgggc tttgccaggt ggctttggtg
    24421 aagtcggcta ttctccaaca gaaaatattc tcaaggaaat tgaagaagaa accggtttta
    24481 aagccaaagt tgaaagactg ctagctgttt ttgataccaa tcgtttccaa ctacagagca
    24541 aacagtatac aaagtttgtt tttggatgta agcttcttga tggacaattc caagaaaatc
    24601 aagaaattgc tgaccttcaa ttttttgcca ttgaccaact gccgaactta tctgaaaaac
    24661 gcattaccaa ggagcaaata gagcttcttt ggcaggttta tcaaggtcat agggggcaat
    24721 atcttgacta agaagatgat tatcgtattt ctaaatccat ttttaacaac tagcatggta
    24781 taataatatg caggaaaatt ttgaattatg aggaagacta gatgaattta tgggatattt
    24841 tctttacgac tcaggcaacc gagccgccca aatttgacct tttttggtat gttagcctat
    24901 ttacgctctt agccttaacc ttttatacag cccatcgcta tcgtgaaaag aaggtttacc
    24961 aacgattttt ccaaatcttg cagactgttc agttaatcct tctttatggt tggtactggg
    25021 tcaatcatat gccactgtca gaaagcctac ccttttacca ttgccgtatg gctatgtttg
    25081 tggtactctt gcttcctggt caatccaaat ataaacaata ctttgcatta ttgggaacat
    25141 ttgggacatt agcagccttt gtttatccag tgccagatgc ttaccctttt ccacatatca
    25201 ccattctatc ctttatcttt ggtcatttag cactcttggg gaactctcta gtttatctat
    25261 tgagacagta taatgcgcga ttgctggatg tgaagggaat ttttctcatg acctttgccc
    25321 taaatgcctt gatttttgtg gtcaatttgg tgacaggtgg cgattacgga tttttgacaa
    25381 aaccgccatt ggttggggat cacggtctag tagctaatta tttacttgtt tcaattgtgc
    25441 tggtagctac tatcagtttg actaagaaaa tcttagaatt ctttttagct caagaagcag
    25501 aaaaaatgat tgcaaaggaa gcttaacaca gagctttctt ttttgctctt agagagtttt
    25561 tacaagcagc ttataaaata agaatttctg aatagacaaa ctcaaaaaat ggctgggaaa
    25621 tttaggaaaa aagcaagcac gattaaattt tttgtgttat aatattttgt gaatagctat
    25681 gcctatgttt agctatggaa taatacgaag tgcgaaactt ggaagataga gaggaagcga
    25741 tgtaatggct agagaaggct tttttacagg tctagatatt ggaacaagct ctgtcaaggt
    25801 gcttgtggcc gagcagagaa atggtgaatt aaatgtaatt ggcgtgagta atgccaaaag
    25861 taaaggtgta aaggatggaa ttattgttga tattgatgca gcagcaactg ctatcaagtc
    25921 agccatttcc caagcggaag aaaaggcagg catttcgatt aaatcagtga atgtcggctt
    25981 gcctggtaat cttttgcagg tagaaccaac tcaggggatg attccagtaa catctgatac
    26041 taaggaaatt acggatcaag atgttgaaaa tgttgtcaaa tcagctttga caaagagtat
    26101 gacacctgac cgtgaagtca ttacctttat tcctgaagaa tttattgtgg atggtttcca
    26161 agggattcgt gacccacgtg gcatgatggg ggttcgcctt gaaatgcgtg gtttgcttta
    26221 tacaggacct cgtactatct tgcacaattt gcgtaagacg gttgagcgtg caggtgttca
    26281 ggttgaaaat gttatcattt caccactagc aatggttcag tctgttttga acgaagggga
    26341 acgtgaattt ggtgctacag tgattgatat gggggcaggt caaacgactg tcgctacaat
    26401 ccgtaatcaa gaactccagt tcacacatat tctccaagaa ggtggagatt atgtaactaa
    26461 agatatctcc aaggttttga aaacctctcg caaattagcg gaaggcttga aactgaatta
    26521 cggggaagcc tatccgcctc ttgcaagcaa agaaaccttc caagtagagg ttattggaga
    26581 agtagaagca gtcgaagtga cggaagccta cttgtcagaa attatttctg cacgaatcaa
    26641 gcacatcctt gaacaaatca agcaagaatt agatagaagg cgtctattgg acctccctgg
    26701 tggtattgtc ttaatcggtg ggaatgccat tttaccaggt atggttgagc ttgctcagga
    26761 agtctttggc gtccgtgtca agctttatgt tccaaatcaa gttggtatcc gtaatccagc
    26821 ctttgcgcat gtgattagtt tatcagaatt tgcgggtcaa ttaacagaag ttaatctttt
    26881 ggctcaggga gcgataaaag gtgagaatga cttaagtcat cagccaatta gttttggtgg
    26941 gatgctgcaa aaaacagctc agtttgtaca atcaacgcct gttcaaccag ctcctgctcc
    27001 agaagtagag ccggtggcgc ctacagaacc aatggcggat ttccaacaag cttcacaaaa
    27061 taaaccgaaa ttagcagatc gtttccgtgg attgatcgga agcatgtttg acgaataaag
    27121 aggaaaaata aattatgaca ttttcatttg atacagctgc tgctcaaggg gcagtgatta
    27181 aagtaattgg tgtcggtgga ggtggtggca atgccatcaa ccgtatggtc gacgaaggtg
    27241 ttacaggcgt agaatttatc gcagcaaaca cagatgtaca agcattgagt agtacaaaag
    27301 ctgagactgt tattcagttg ggacctaaat tgactcgtgg tttgggtgca ggaggtcaac
    27361 ctgaggttgg tcgtaaagcc gctgaagaaa gcgaagaaac actgacggaa gctattagtg
    27421 gtgccgatat ggtcttcatc actgctggta tgggaggagg ctctggaact ggagctgctc
    27481 ctgttattgc tcgtatcgcc aaagatttag gtgcgcttac agttggtgtt gtaacacgtc
    27541 cctttggttt tgaaggaagt aagcgtggac aatttgctgt agaaggaatc aatcaacttc
    27601 gtgagcatgt agacactcta ttgattatct caaacaacaa tttgcttgaa attgttgata
    27661 agaaaacacc gcttttggag gctcttagcg aagcggataa cgttcttcgt caaggtgttc
    27721 aagggattac cgatttgatt accaatccag gattgattaa ccttgacttt gccgatgtga
    27781 aaacggtaat ggcaaacaaa gggaatgctc ttatgggtat tggtatcggt agtggagaag
    27841 aacgtgtggt agaagcggca cgtaaggcaa tctattcacc acttcttgaa acaactattg
    27901 acggtgctga ggatgttatc gtcaacgtta ctggtggtct tgacttaacc ttgattgagg
    27961 cagaagaggc ttcacaaatt gtgaaccagg cagcaggtca aggagtgaac atctggctcg
    28021 gtacttcaat tgatgaaagt atgcgtgatg aaattcgtgt aacagttgtt gcaacgggtg
    28081 ttcgtcaaga ccgcgtagaa aaggttgtgg ctccacaagc tagatctgct actaactacc
    28141 gtgagacagt gaaaccagct cattcacatg gctttgatcg tcattttgat atggcagaaa
    28201 cagttgaatt gccaaaacaa aatccacgtc gtttggaacc aactcaggca tctgcttttg
    28261 gtgattggga tcttcgccgt gaatcgattg ttcgtacaac agattcagtc gtttctccag
    28321 tcgagcgctt tgaagcccca atttcacaag atgaagatga attggataca cctccatttt
    28381 tcaaaaatcg ttaagtaaat gaatgtaaaa gaaaatacag aacttgtttt tcgagaagtt
    28441 gcagaggcta gtctgagtgc tcatcgagag agtggttcgg tctctgtcat tgcagttacc
    28501 aagtatgtag atgtaccgac agcggaagcc ttgcttccgc taggtgtcca tcatatcggt
    28561 gaaaatcgtg tagataagtt tctggaaaaa tatgaagctt taaaagatcg agatgtgact
    28621 tggcatttga ttggtacctt gcaaagacgt aaggtgaaag atgtcattca atacgttgat
    28681 tatttccatg cattggactc agtaaagcta gcaggggaaa ttcaaaaaag aagtgaccga
    28741 gtcatcaagt gtttccttca agtaaatatt tctaaagaag aaagcaaaca cggtttttcg
    28801 agagaggaac tgctggaaat cttgccagag ttagccagac tagataagat tgaatatgtt
    28861 ggtttaatga cgatggcacc ttttgaggct agcagtgagc agttgaaaga gattttcaag
    28921 gcggcccaag atttacaaag agaaattcaa gagaaacaaa ttccaaatat gcctatgacc
    28981 gagttaagta tgggaatgag tcgtgattat aaagaagcga ttcaattcgg ttccactttt
    29041 gttcgtatag gtacatcatt ttttaagtag gagagaacca tgtctttaaa agatagattc
    29101 gatagattta tagattattt tacggaggat gaggattcaa gtctccctta tgaaaaaaga
    29161 gatgagcctg tgtttacttc agtaaattct tcacaggaac cggctctccc aatgaatcaa
    29221 ccttcacagt cggctggcac aaaagagaac aatatcacca gacttcatgc aagacaacag
    29281 gaattggcaa atcagagtca gcgtgcaacg gataaggtca ttatagatgt tcgttatcct
    29341 agaaaatatg aggatgcaac agaaattgtt gatttattgg caggaaacga aagtatcttg
    29401 attgattttc agtatatgac agaggtgcag gctcgtcgtt gtttggacta tttggatgga
    29461 gcttgtcatg ttttagctgg aaatttgaaa aaggtagctt ctaccatgta tttgttgaca
    29521 ccagtgaacg ttattgtaaa tgttgaagat atccgtttac cagatgaaga tcaacagggt
    29581 gagttcggtt ttgatatgaa gcgaaataga gtacgataat gattttttta attcgtatga
    29641 tttataatgc agtggatatt tactccctga ttttggtagc cttcgctgtc atgtcttggt
    29701 ttccaggtgc ctacgaatcc agtttaggtc gttggattgt agcgttggtg aaaccagtgc
    29761 ttgctccctt gcaacgcctg cctttacaga tagcgggtct tgatttatct gtttgggttg
    29821 cgattgtttt ggttcgattt ttaggagaaa acctagtgcg ttttctggcg atgataggat
    29881 gaataaaggg atttatcagc atttctccat agaagatcgt ccatttcttg acaagggaat
    29941 ggaatggata aagaaggtag aagatagcta tgctcctttt ttaactcctt ttatcaatcc
    30001 tcatcaggag aagctattaa agattttggc caaaacctat ggtcttgctt gtagcagtag
    30061 tggggaattc gtctcgagtg agtatgttcg agttttatta tacccagatt atttccaacc
    30121 agagttttca gattttgaaa tatctctcca ggaaattgtg tattccaata aatttgaaca
    30181 tttaacgcat gctaagattt tagggacagt catcaatcaa ttagggattg aacggaaact
    30241 ttttggagat atcctagtag atgaagaacg ggcgcagatt atgattaatc agcagtttct
    30301 tcttctcttt caagatggac taaagaaaat tggtcgtata cctgtttcgc tggaggaacg
    30361 tcctttcacc gagaaaatag ataagctaga acagtatcga gaactggatt tatctgtgtc
    30421 tagttttcga ttagatgttc ttttatcaaa tgttttgaaa ctatctagga atcaagcaaa
    30481 ccagttgatt gaaaagaaac ttgtccaagt aaattatcat gtggtagaca aatcagatta
    30541 cactgttcaa gttggagact tgattagtgt gagaaaattt ggtcgcttga gattacttca
    30601 agataaggga caaacgaaaa aagagaagaa aaaaataacc gtccagttat tattaagtaa
    30661 gtgaggaata gaatgccaat tacatcatta gaaataaagg acaagacttt tggaactcga
    30721 ttcagaggtt ttgatccaga agaagtcgat gaatttttag atattgtggt tcgtgattac
    30781 gaagatcttg tgcgtgcgaa tcatgataaa aatttgcgta ttaagagttt agaagagcgt
    30841 ttgtcttact ttgatgaaat aaaagattca ttgagccagt ctgtattgat tgctcaggat
    30901 acagctgaga gagtgaaaca ggcggcgcat gaacgttcaa acaatatcat tcatcaagca
    30961 gagcaagatg cgcaacgctt gttggaagaa gctaaatata aggcaaacga gattcttcgt
    31021 caagcaactg ataatgctaa gaaagtcgct gttgaaacag aagaattgaa gaacaagagc
    31081 cgtgtcttcc accaacgtct caaatctaca attgagagtc agttggctat tgttgaatct
    31141 tcagattggg aagatattct ccgtccaaca gctacttatc ttcaaaccag tgatgaagcc
    31201 tttaaagaag tggttagcga agtacttgga gaaccgattc cagctccaat tgaagaagaa
    31261 ccaattgata tgacacgtca gttctctcaa gcagaaatgg cagaattaca agctcgtatt
    31321 gaggtagccg ataaagaatt gtctgaattt gaagctcaga ttaaacagga agtggaagct
    31381 ccaactcctg tagtgagtcc tcaagttgaa gaagagcctc tgctcatcca gttggcccaa
    31441 tgtatgaaga accagaagta gctccaatgc atccgatagg tccaacacca gctacagaaa
    31501 ctgttgattc aataccggga tttgaagcac cgcaagaatc tgttacaatt ttataagaaa
    31561 tattctgaga acaatatctt atccttatat ttccagcgag caggagatgg tgtgagtcct
    31621 gtaatcccta ttgataagat tatcctctca aaaactcaag tctgaagcta gtaagatttg
    31681 acgtttccca cgttacggga taagagggag aaagactaaa tctttttccg aataaaggtg
    31741 gtaccacgat tttcgtcctt tttggaagtc gtggttttta atttgttatt atttataaag
    31801 gagataccat gaaactcaaa gacaccctta atcttgggaa aactgaattc ccaatgcgtg
    31861 caggccttcc taccaaagag ccagtttggc aaaaggaatg ggaagatgca aaactttatc
    31921 aacgtcgtca agaattgaac caaggaaaac ctcatttcac cttgcatgat ggccctccat
    31981 acgctaacgg aaatatccac gttggacatg ctatgaacaa gatttcaaaa gatatcattg
    32041 ttcgttctaa gtctatgtca ggattttacg caccatttat tcctggttgg gatactcatg
    32101 gtctgccaat cgagcaagtc ttgtcaaaac aaggtgtcaa acgtaaagaa atggacttgg
    32161 ttgagtactt gaaactttgc cgtgagtacg ctctttctca agtagataaa caacgtgaag
    32221 attttaaacg tttgggtgtt tctggtgact gggaaaatcc atatgtgacc ttgactcctg
    32281 actatgaagc agctcaaatt cgtgtatttg gtgagatggc taataagggt tatatctacc
    32341 gtggtgctaa gccagtttac tggtcatggt catctgagtc agcacttgct gaagcagaga
    32401 ttgaatacca tgacttggtt tcaacttccc tttactatgc caacaaggta aaagatggca
    32461 aaggagttct agatacagat acttatatcg ttgtctggac aacgactcca tttaccatca
    32521 cagcttctcg tggtttgacg gttggtgcag atattgatta cgttttggtt caacctgctg
    32581 gtgaagctcg taagtttgtc gttgctgctg aattattgac tagcttgtct gagaaatttg
    32641 gctgggctga tgttcaagtt ttggaaactt accgtggcca agaactcaac cacatcgtaa
    32701 cagaacaccc atgggataca gctgtagaag agttggtaat tcttggtgac cacgttacga
    32761 ctgactctgg tacaggtatt gtccatacag cccctggttt tggtgaggac gattacaatg
    32821 ttggtattgc taataatctt gaagtcgcag tgactgttga tgaacgtggt atcatgatga
    32881 agaatgctgg tcctgaattt gaaggtcaat tctatgaaaa ggtagttcca actgttattg
    32941 aaaaacttgg taacctcctt cttgcccaag aagaaatctc tcactcatat ccatttgact
    33001 ggcgtactaa gaaaccaatc atctggcgtg cagttccaca atggtttgcc tcagtttcta
    33061 aattccgtca agaaatcttg gacgaaattg aaaaagtgaa attccactca gaatggggta
    33121 aagtccgtct ttacaatatg atccgtgacc gtggtgactg ggttatctct cgtcaacgtg
    33181 cttggggtgt tccacttcct atcttctacg ctgaagatgg tacagctatc atggtagctg
    33241 aaactattga acacgtagct caactttttg aagaatatgg ttcaagcatt tggtgggaac
    33301 gtgatgccaa agacctcttg ccagaaggat ttactcatcc aggttcacca aacggcgagt
    33361 tcaaaaaaga aactgatatc atggacgttt ggtttgactc aggttcatca tggaatggag
    33421 tggtggtaaa ccgtcctgaa ttgacttacc cagccgacct ttacctagaa ggttctgacc
    33481 aataccgtgg ttggtttaac tcatcactta tcacatctgt tgccaaccat ggcgtagcac
    33541 cttacaaaca aatcttgtca caaggttttg cccttgatgg taaaggtgag aagatgtcta
    33601 aatctcttgg aaatactatt gctccaagcg atgttgaaaa acaattcggt gctgaaatct
    33661 tgcgtctctg ggtaacaagt gttgactcaa gcaatgacgt gcgtatctct atggatatct
    33721 tgagccaagt ttctgaaact taccgtaaga ttcgtaacac tcttcgtttc ttgattgcca
    33781 atacatctga ctttaaccca gctcaagata cagtcgctta cgatgagctt cgttcagttg
    33841 ataagtacat gacgattcgc tttaaccagc ttgtcaagac cattcgtgat gcctatgcag
    33901 actttgaatt cttgacgatc tacaaggcct tggtgaactt tatcaacgtt gacttgtcag
    33961 ccttctacct tgattttgcc aaagatgttg tttacattga aggtgccaaa tcactggaac
    34021 gccgtcaaat gcagactgtc ttctatgaca ttcttgtcaa aatcaccaaa ctcttgacac
    34081 caatccttcc tcacactgcg gaagaaatct ggtcatatct tgagtttgaa acagaagact
    34141 tcgtccaatt gtcagaatta ccagaagttc aaacttttgc taaccaagaa gaaatcttgg
    34201 atacatgggc agccttcatg gactttcgtg gacaagcaca aaaagccttg gaagaagctc
    34261 gtaatgcaaa agttatcggt aaatcacttg aagcacactt gacagtttat ccaaatgaag
    34321 ttgtgaaaac tctactcgaa gcagtaaaca gcaatgtagc acaacttttg atcgtgtctg
    34381 agttgaccat cgcagaagga ccagctccgg aagctgccct tagcttcgaa gatgtagcct
    34441 tcacagttga acgtgctact ggtgaagtat gtgaccgttg ccgtcgtatc gacccaacaa
    34501 cagcagaacg cagctaccag gcagttatct gtgaccactg tgcaagcatc gtagaagaaa
    34561 actttgcgga agcagtcgca gaaggatttg aagagaaata agattgaaaa gtctaggcaa
    34621 aattcaattt gagaagaaaa gacaactaat tttatagtct attaaacgca ttgtatcacg
    34681 tttttgaata cctgatatga tgcgtttttt atttatttta aaaatttgcg aggtatgact
    34741 ttttatactc aacaagaatc aaagagaaac ttagcaagct aacagtagta agataaaata
    34801 ggaatttgat attagggata agattggtaa atagtgtaat atttttacaa caataaattt
    34861 atatagttat ttctggtttc tgaaaagtat tatattttat ttcatattat acaaattttt
    34921 attttataat atcagaacat acttttttta aaagcaaata tgatacaatt ttatttgaaa
    34981 aaaataaaaa aggagatttt attataaaat taaaaagact tgctttaatt agtggtatcg
    35041 tcggtcttgt gggaggaatt ttacttctta ttggtccttt tgtcttgttg ggaatagcgg
    35101 taaacacagc tgctacaact cttaatggag gagctactgc aggggctttt tcaggtgtag
    35161 ccttactctt gaatgccttg aagattgcaa atcttgttct tggtatcatt gctattgttt
    35221 actataaagg agataagcgt gtaggtgcag ctccgtctgt actaatgatt gtttctggtg
    35281 gagttagtct cattctattc cgttcttagg atgggttggg gggatttttg ctattatcgg
    35341 aggatctcta ttcctttcaa cattgaagaa attcaaatca gaagaataaa aggtatttta
    35401 gcatgaaaag aacaaaaaag tttatcggta taggagtagc tctattatct ctttctcttc
    35461 tagttgcatg tggaacataa agttcaaaga atacttcaac aagtaatgat gagaagacag
    35521 tagcaacatc caatagttca aaagaaacaa tcactttcga tacaccggtt gtaacagacg
    35581 atgcgattga atcaatacgc acttatgcag attatataga tctttataaa aatatttttg
    35641 atgattattt tactaaagct gaggaaggtt tcaaaggcat agctatggaa aataatgact
    35701 cgtttactaa actaaaagag tcaactcaaa aattattcga tgcgcagaaa aaaaggttaa
    35761 ataatgaaga tagaatagaa acaaccaaaa acaatgtgat tgccaaacat tgtcaaacag
    35821 tcctttcctt tttggttttg actagctttt ttgtgaaaaa ttgtgtaaaa tagaatagat
    35881 aaacgagggg aaacctcgga aaatttaaag gagaatccat ctaatggtaa aattggtttt
    35941 tgctcgccac ggtgagtctg aatggaacaa agctaacctt ttcactggtt gggctgatgt
    36001 tgatttgtct gaaaaaggta cacaacaagc gattgacgct ggtaaattga tcaaagaagc
    36061 tggtatcgaa tttgaccaag cttacacttc agtattgaaa cgtgctatca aaacaactaa
    36121 cttggctctt gaagcttctg accaattgtg ggttccagtt gaaaaatcat ggcgcttgaa
    36181 cgaacgtcac tacggtggtt tgactggtaa aaacaaagct gaagctgctg aacaatttgg
    36241 tgatgagcaa gttcacatct ggcgtcgttc atacgatgta ttgcctccaa acatggaccg
    36301 tgatgatgag cactcagctc acacagaccg tcgttacgct tcacttgacg actcagttat
    36361 cccagatgct gaaaacttga aagtgacttt ggaacgtgct cttccattct gggaagataa
    36421 aatcgctcca gctcttaaag atggtaaaaa cgtattcgta ggagctcacg gtaactcaat
    36481 ccgtgccctt gtaaaacaca tcaaaggttt gtcagatgac gagatcatgg acgtggaaat
    36541 ccctaacttc ccaccattgg tattcgaatt cgacgaaaaa ttgaacgtcg tttctgaata
    36601 ctaccttgga aaataaaaaa ttgtaagtct agaattgatt tctaggcttt ttatgttagt
    36661 atggaagtat gataaggaat aaaaaacaag attatgtact ggcctacaag caaccagctt
    36721 caaccactta catgggttgg gaagaagaag ctttaccgat aggcaatggt tctttaggag
    36781 caaaagtatt tggccttata ggggctgaac ggattcaatt taatgaaaaa agtctctggt
    36841 ctggaggtcc acttcctgat agttcagatt atcagggtgg aaatcttcag gatcagtatg
    36901 tttttttagc tgagattcgg caggctttgg agaagagaga ttacaatctg gctaaggaac
    36961 tggctgagca gcacctaatt gggccaaaaa cgagtcaata tgggacctat ctgtcttttg
    37021 gggatattca cattgagttc agccagcaag gtacgacttt gtctcaggtg acggactatc
    37081 agagacagct gaatattagt aaggcacttg cgacgacttc ttatgtctat aagggaacgc
    37141 gatttgaacg taaagctttt gcgagttttc cagatgatct cttggttcaa tgttttacta
    37201 aggaagggtt ggaaactcta gattttacta tagaactatc cttgacctgt gatttggctt
    37261 ctgatggaaa gtatgagcag gaaaaatctg attacaagga gtgtaagttg gatattactg
    37321 attctcatat cttgatgaag ggaagagtta aggataatga tctgcggttt gctagttatc
    37381 tagcttggga aacggatgga gatattagag tttggtcaga tagggttcag atatcaggag
    37441 ccagttatgc caatctcttc ttggccgcta agacggattt tgcccaaaat cctgctagca
    37501 attatcgcaa gaaactagat ttagagcaac aggtgataga cttggtggac acagctaaag
    37561 aaaagggcta tacccaattg aaatcaaggc atatcgagga ctaccaagcc ttattccagc
    37621 gtgttcaatt ggatttggaa gctgatgttg acgcatccac tacagatgat ttgttaaaaa
    37681 attataagcc acaagaaggg caggctttgg aggagctgtt cttccagtat ggacggtatt
    37741 tattgattag ttcgtccaga gactgcccag atgctctacc agctaaccta cagggagtct
    37801 ggaatgcggt cgacaatcct ccttggaatt cggactatca cttaaatgtc aatctgcagc
    37861 tgaattattg gccagcctat gttaccaatc tcctagagac ggtctttcca gtcatcaact
    37921 atgtagatga tttgcgtgtc tatggtcgtc tagcggctgt aaagtatgca ggaatcgtct
    37981 ctcagaaagg tgaggagaat ggttggttgg ttcatactca agcgactccc tttggttgga
    38041 cggcacctgg ttgggattac tattggggtt ggtcaccagc tgccaatgcg tggatgatgc
    38101 aaaccgttta tgaagcctat ttattttata gggaccaaga ctatctcagg gagaaaattt
    38161 atcccatgtt gagggaaacg gttcgttttt ggaatgcctt tttacataag gatcagcagg
    38221 cgcagcgttg ggtgtcttct ccgtcttatt ccccagaaca tgggccgatt tcgattggca
    38281 atacctatga ccaatctctg atttggcagt tatttcatga ttttattcag gctgctcagg
    38341 aattgggact ggatgaggac ttgttgactg aggttaagga gaagtctgat ttactaaatc
    38401 ctttgcaaat cactcaatct ggtcgaatca gggagtggta tgaggaggaa gagcagtatt
    38461 ttcaaaatga gaaagtggag gcccagcatc ggcacgcttc ccatctagtg ggactctatc
    38521 ctggcaatct ctttagctac aagggacaag agtatattga agcggcgcgt gctagcctca
    38581 atgatcgtgg agatggcggc acaggctggt ccaaggctaa taagatcaat ctctgggcgc
    38641 gtttgggaga tggcaatcga gcccataaat tattggcaga gcagttaaag acatccacct
    38701 tgcaaaatct ttggtgtagc catcctcctt ttcagataga tggtaatttt ggtgctacta
    38761 gtggcatggc agaaatgtta ctccagtctc atgcagctta tctggtacct ctagctgccc
    38821 tacctgatgc ttggtcaaca ggttctgttt caggcttaat ggcacgtgga cattttgaag
    38881 tgagcatgag ctgggaagat aaaaaactct tacagttgac cattttatca aggagtggag
    38941 gagatttgcg agtttcttat ccagatattg agaagagtgt gattaaaatg aatcaagaaa
    39001 aaataaaagc gaaatgcatg gggaaagatt gtatttcggt ggcaacagca gaaggtgatc
    39061 ttgttcaatt ttatttttaa gaagatgtta taaggcagta atttgaaact gccttttaat
    39121 aaggatttaa gaatataagc agttttcaac tagttgaaaa aacgttataa tgataatagg
    39181 aagtaatact caatgaaaat caaagagcac aaactaggaa gctagccgca ggttgctcaa
    39241 aacagtgttt tgaggttgca gatggaagct gacgtggttt gaagagagat tttcgaggag
    39301 tataatttgt ttgatagagg gtgggtctga tggcttatat tgagatgaaa cactgttaca
    39361 agcgttatca ggttggggac acggagattg tggccaattg tgatgtgaat tttgagattg
    39421 aaaaggggga gctggttatt atccttggtg cttcaggtgc aggcaagtca acagttctta
    39481 accttcttgg gggaatggat accaatgatg aaggggaaat ctggattgat ggtgttaata
    39541 ttgcggatta tagttcccac cagcgcacca attaccgtag aaatgatgtg gggtttgttt
    39601 ttcagtttta taatctagtt tctaatctga cagctaagga aaatgtggaa ctggcttctg
    39661 aaattgtgac agatgccttg aatcctgatc aggccttgac agatgtaggt ctggctcatc
    39721 gtctcaataa ctttccagcc cagctttctg gaggggagca acagcgagtc tccattgcac
    39781 gcgcggtagc caaaaatcct aaaattctcc tttgtgatga accgactgga gccttggatt
    39841 atcagacggg caagcaggtt ttgaaaattc tccaagacat gtctcgtcaa aagggagcga
    39901 cggtgatcat cgtgactcat aatggagctt tggcgcccat tgctgatcgc gtgattcaaa
    39961 tgcacgatgc cagtgtcaag gatgtggtgc tcaaccagca tcctcaggat attgacagtt
    40021 tggagtacta gcatgatcaa gcgaaaaact tattggaagg acttagttca gtccttcaca
    40081 ggctccaagg ggcgtttttt atccatcttg atcctgatga tgttgggatc tctagcctta
    40141 gtaggcctca aagtaaccag tcccaacatg gaggcgacag ctaatgctta tttaacaact
    40201 gctcaaacct tggatttggc agtcatgtct aactatggct tggatcaagc agaccaagaa
    40261 gaactaaaac agacggaggg cgcagaggtc gagtttggct atttgacaga tgtgactatg
    40321 gataatgggc aggatgccat tcggctgtac tccaaaccag agcgaatttc aacctttcag
    40381 ctaagaaagg gacgacttcc tcagtcagac aaggaaatcg ctttggccac tcatttgcaa
    40441 ggccaataca gcgtgggaca ggagattagt tttaaagaaa aagaagaggg tcattcctct
    40501 ttaaaagacc atacttatac cattactggt tttgtggatt cggctgaaat cctctcccag
    40561 cgagatatgg gctacgcagg aagtggaagt gggactctga cagcctatgg ggtgatttta
    40621 cctagtcaat ttgatcagaa agtctacaat atagctcgtt tgaaatatca agatttagcg
    40681 ggtttaaatg ccttttcatc agcttatgaa gaaaaatcca agcaacatca agaagagctt
    40741 gaacaaattt tatcagataa tggcaaggta cgtctgcaac ttttgaaaaa agaaggacaa
    40801 gagtctctag acaaggggca agagaccctt gacaaggctc agactaattt gcaggaaggc
    40861 aagcgtcgtt tagcagctgc tcaagctcgt atacaggctc aagaaagtca actagccttg
    40921 tttcctcaag ttcagagaga gcaggctagt gctcaactta cccaagccaa gcaggaattg
    40981 ggcaaggaag aggacaaact aaagcaagct gaacaaaatc tagcccaaga aaaggaaaaa
    41041 ttagaaaaac atcagcaagt cttggatgat ttggcggagc caaggtatca ggtttataat
    41101 cgtcagacca tgccaggtgg tcagggctat cttatgtata gcaatgcttc atccagtatt
    41161 cgagcagtgg gcaatatctt tcctgtggta ctttatgccg tagcagccat ggtgaccttt
    41221 acgaccatga ctcgctttgt agacgaagag cgaactcatg cagggatttt taaggccttg
    41281 ggttatcgta gtaaggatat tatcgccaag tttctccttt atggactagt agctgggact
    41341 gtcggaacgg ctctaggtag tatacttggt cattatttgc tagccagtgt aatttcaagt
    41401 gtcattacaa aaggcatggt ggtgggagaa actcagattc agttctattg gacctatagc
    41461 ttactagctt ttgtcttgag cttgttggcg agtgtgttac cagcctatct ggtggcttgg
    41521 agggaacttc atgacgaagc agcccagctt ctacttccta aacctcctgt caaaggagct
    41581 aaaatcttat tggagcgtat cggttttatc tggcgtcgtc tcagttttac tcataaggta
    41641 acagcccgca acatctttcg ttataagcag agaatgttga tgacaatctt tggtgtggca
    41701 ggttctgtag ctctgctctt tgcaggtttg ggaatccaat cttctgtagc aggagttccg
    41761 tctaaacagt ttcaacaaat ccaacagtat cagatgcttg tctctgaaaa tcctagtgcg
    41821 accaatcagg acaaggtaga gctagcagaa gtgttgaaag ggcaggagat actagcctac
    41881 cagaaaatct attctaaagc gctatacaag gatttcaaag gcaaagctgg tcttcaaaac
    41941 attactctta tgatgataga gaaggaagat ttgactccct ttatccatct tcaacatcat
    42001 cagcaggagc tgacattaaa agatggcatc gttattacag ctaaactcgc ccagctggca
    42061 ggtgtcaagg ttgggcagac tttagaaatt gaaggtaagg aactaaaggt cgttgctatt
    42121 actgagaact acgttggtca ctttatttat atgagtcagg ctagctatga gcaactttac
    42181 ggacagctac cccaagccaa cacttatctg gtctcattaa gggataccag tgcaactagt
    42241 atcgaaagtc aggcgggctt gcttatgaat caatctgcgg tgtccagcgt tgtccaaaat
    42301 gcttcagcca ttcgactctt cgactctatc gctagctcac tcaatcagac catgaccatc
    42361 ttggtcatcg tatcggttct attagctatt gtcatccttt acaatctgac caatatcaac
    42421 gtagctgaga gaatccgtga actctccact atcaaggttc ttggttttca taataatgaa
    42481 gtcaccctct acatttaccg tgagacgatt gtgctgtccc ttgtgggaat cgtacttggt
    42541 ctgatagctg gtttctattt acaccaattt ttgattcaaa tgatttcgcc tgcgactatt
    42601 ctcttttatc cgcaggtagg ctgggaagtc tatgtaatcc cagtggcagc agtaagcatc
    42661 attttgacct tgcttggttt cttcgtcaat tattatctga gaaaggttga tatgttagaa
    42721 gccctgaaat ctgtagagta aggtagttat ttttagctga ttgaacttct atttactaat
    42781 attcaaaaat cctccgtttc aaagagcagg gaactctttg tgacagagga ttttttctat
    42841 agggctttag cagctgcaat tgcggcttcg aagtttggct cagaattgat attatccacg
    42901 tattcaacgt agcgaatcgt attgtcagta tcgaggacaa agactgcgcg tgctaatagg
    42961 tgccattcgt tgatcaagag ggcataatcg cgcccgaaag aatggtcaaa gtagtctgaa
    43021 agcataatgg cattgtcaag gccttcagca ccgcaccaac gtttttgagc aaaaggtagg
    43081 tccattgaaa cagtcaatac gaccgtgttg tccagtccag ccaattcttc attaaaacga
    43141 cgtgtttgag ttgagcagat gcctgtatcg atagaaggaa cgacactcaa gacttttttc
    43201 ttgccatcaa aatcagccag agatttttta gaaagatctg ttgtagtaag agaaaaatca
    43261 agcgccttgt cgccgacttg tagttgttta cctgtaaagc tcacaggatt tccgagaaaa
    43321 gttaccatag gatactccaa tcttttttct tccattttag ctgaaacagt cggaattttc
    43381 caatgatttg accggaaata tgggcataga aaaaacgcca gctcatgtga gaatgacgtt
    43441 tttcataggt ttattttgcc aatccttcag caatcttgtc aaggttgtat ttcatcatgc
    43501 tgtagtagct gtcgccttct ttaccttgtt ctgcgataga gtcagtaaag atttgagcgt
    43561 agattgggat gtttgtgtct tgagaaacag ttttcattgg acggtcatcc acacttgatt
    43621 ctacaaagag tgatggaact tttgtttggc gaagtttttc aaccaaggtc ttgatttgtt
    43681 caggagttcc ttcttcttca gtattgattt cccagatgta agcacttggg acaccatagg
    43741 ctttagagaa gtatttgaat gctccttcgc tggttacaat gagtttcttt tcagcaggga
    43801 tcttattaaa tttatcctta ctttctttat caagtttgtc taacttatca gtatattctt
    43861 tgagattttt ttcatagaat tctttattgt tagggtcttt ggcgctcaat tgtttggcga
    43921 tatttttagc aaaaataata ccgttttcaa ggttaagcca agcgtgtggg tcttcttttc
    43981 ctttttcatt ttgaccttca aggtagataa catcaacgcc gtcgctgact gcgaagtagt
    44041 ctttgttttc agttttcttg gcattttcta ccaattttgt aaaccaagca ttgccacctg
    44101 tttcaaggtt gataccgtta tagaaaatca aattagcctc agaagttttc ttaacgtctt
    44161 caggaagtgg ttcgtattcg tgtgggtctt gcccaatcgg aacgatacta tgaaggtcaa
    44221 ttttgtcacc agcaatattt ttagtaatat cagcgatgat tgagtttgta gcaacaactt
    44281 ttagtttttg accagaagtt gtatcttttt ttccgctagc acatgctaca agaatgattg
    44341 cagaaagaaa gagaacgagt aatgtaccta attttttcat tagatcctcc aatttattag
    44401 ggctttgccc cttattttaa caaatgttta tttttcagtt tcaaatatcg ttgtttggga
    44461 gcgataaaga agctaatgag aaagaaacta gcagctgtaa gcacgatact agaacctgcc
    44521 gcaacattaa aactatagcc aataaagagt cccaaaactg aagcagtagc tccgaaggtt
    44581 gaggaaagga aaatcatact tttcagacta ttagcataca gataagcagt tgcagctggg
    44641 gtaatcagca tggctacaat caggatagtt ccgacacttt gcatggctgt cacagacacg
    44701 agagtcagga gtaccatgag aaggtagtga tagaaattga caggcattcc catggcttta
    44761 gccaagagtt catcaaagga agttatcaag agttgcttga agaaaatcca gattaacaag
    44821 aggatagctg cccccacacc catagtaata aacatatccg tatcttggac ggccaggata
    44881 ttaccaaaaa ggatatggaa aaggtcagtt gaacttttag cgacaccaat caagatgata
    44941 ccgagggcta agaaagaaga aaaggtaatg ccgatggcgg tatcgctttt gataatcgag
    45001 tttcctttga tgtaggtaat gatgatggca gctagcaatc caaagacaat ggctccgata
    45061 aagaagtcaa ggcccaagat gaaggatagg gctacacctg gtaagacagc atgtgaaatg
    45121 gcatctccca tgagtgacat cccgcgtaga ataatgaaac atcccacagc tccagctaca
    45181 atcccgacga caatagctgt tatcaaggca ttttgtagga aatggaattt ttgcaatcca
    45241 tcgataaatt ctgcaatcat aggtcacctc cattgaaaaa gagttgatta ccgtaagctt
    45301 cttttagatt ggtttcggta aaagtttctt ttgttggacc aaaggcaatc acttctcgat
    45361 tgacaagtaa gacttgatcg aagtagtggg gaatcttgct gaggtcgtgg tgaacgatga
    45421 gaaccgtctt cccagctttt ttcaaatctc tcagcgtatt catgatgatt tcctcactga
    45481 cagagtcaat cccagcaaag ggttcatcca agaggatata gtcggcttcc tgcaccaaac
    45541 atctggcaat caagacccgc tggaattgac ctccagacag ttgactaatt tgacgttcag
    45601 cgtagtcagc taggccgacg atttcaaggg cctcttgcac tttcttccaa tgtttagcct
    45661 ttaaacttcg aaagagagga atagagggaa atagtcctaa cgagacgcat tccttgacct
    45721 tgatgggaaa gttgtagtcg atattgattt tttgttcgac ataggcaatt cggtgtaagg
    45781 attttttaac ttccttgtca tcgagaaatg cctgaccttg atgtgggata attcccaaca
    45841 taccttttaa tagtgttgat ttcccagcgc cgtttggacc aatgatgccg gtaattgttg
    45901 gtccatggag cactagtgaa atatccttaa gtgccaacgt ttctttgtag gagacactga
    45961 ggttttcgat acgtatcata aacttgtatt cctcctgtct cttaatatac attaaaaaaa
    46021 aaattaagtc aagttaattt ttgaaaaaat taaaataata actgaaaaat agattctaaa
    46081 gataactttc aggataaatt tctaaattat aaaacgcata gtatcaagtg taaaaaactt
    46141 ggaattatgc gttttatcat ggaaagattt tttataatag ctaaaaaata atagttaaat
    46201 ggttattaat tgcattccct agtgattttt gttaagataa atgcaaatac aaatgaaagc
    46261 gagaacaaga tgacacgtta tcaagatgat ttttatgatg ctatcaatgg agaatggcaa
    46321 cagacagctg aaatcccagc agataagtct caaacaggag gttttgttga tttagaccag
    46381 gaaattgaag acctgatgct ggcgacaaca gacaagtggt tagcaggtga agaagtgcct
    46441 gaggatgcta tcttggaaaa ctttgtcaaa taccaccgcc tagttcgtga ttttgacaag
    46501 agagaagctg acggtatcac acctgtctta ccactcctta aagaattcca agaattggag
    46561 acttttgcgg attttacagc taaactagca gagtttgagc ttgcaggaaa accaaacttc
    46621 cttccttttg gtgtatcgcc agactttatg gatgctagaa tcaatgttct atgggctagc
    46681 gctccaagca caatcttgcc agatacgacc tactatgcag aagaacatcc tcagcgcgaa
    46741 gagctcttga ctctttggaa agaaagcagc gcaaatctcc tcaaggctta tgatttctct
    46801 gatgaagaaa ttgaagactt gctagaaaaa agacttgaat tggaccgccg agttgcggca
    46861 gtggtgctct ctaatgaaga aagttcagaa tatgctaaac tctatcatcc atattcttac
    46921 gaagatttca agaaattcgc gcctgcccta cctttggatg acttcttcaa agcagttatt
    46981 gggcaattac cagacaaggt tattgtagac gaggaacgtt tctggcaagc agcagagcaa
    47041 ttctacagtg aggaagcctg gtctctcctt aaagcaacct tgattttgag tgttgtcaat
    47101 ctttcaacca gctatttaac agaggatatc cgtgttttgt ctggtgccta cagccgtgcc
    47161 ctttctggag ttccagaggc aaaagataag gtcaaagcag cttatcatct agcacaagaa
    47221 cctttcaagc aagccctggg tctttggtac gcccgtgaga agttctctcc agaagccaag
    47281 gcggatgtgg agaaaaaagt ggcaaccatg attgatgttt ataaggagcg tctgcttaag
    47341 aatgactggc tcactccaga aacctgtaaa caggctatcg tgaagctcaa tgtgatcaaa
    47401 ccttatattg gctatccaga agaattgcct gcacgttaca aggataaggt agtgaatgaa
    47461 actgccagtc tttttgagaa tgctctagcc tttgcgcgtg tggaaatcaa gcacagttgg
    47521 agtaagtgga accagcctgt agattataag gaatggggca tgcctgctca tatggtcaat
    47581 gcctactaca atcctcagaa gaacctgatt gtctttccag cggccatttt acaggcgcct
    47641 ttctatgact tgcatcagtc atcttctgct aactacggtg gtattggggc agtgattgcc
    47701 catgaaattt cccacgcctt tgatactaac ggggcttcct ttgacgaaaa tggtagcctc
    47761 aaggattggt ggacagagag cgactatgct gccttcaagg agaaaacaca aaaagtcatt
    47821 gaccaatttg atggacagga ttcttatgga gcaaccatta acggtaaatt gactgtatca
    47881 gaaaacgtgg ctgacttggg aggaatcgca gcagcgcttg aagcagctaa gagagaagca
    47941 gacttctcag cagaagagtt cttctacaac ttcggtcgca tctggcgcat gaaaggtcga
    48001 ccagaattta tgaaactttt ggctagcgtc gatgtgcacg caccagccaa actccgtgtc
    48061 aatgtgcaag taccaaactt cgacgatttc tttacaacct atgatgtcaa agaaggagac
    48121 ggaatgtggc gttcaccaga ggagcgcgtg attatttggt aatgcaaaaa atctagtcat
    48181 cagaaaaaag gcatccaatc atgtgtgaaa accttgatag gatgcctttt gtaatagaaa
    48241 gatttgctgg atagtttact taaatttcga ttgtcatcat cttgctagaa aaagggattg
    48301 aaggcctttt cgtgagcgat agtagtagct ggaccatgtc ctggatagac atcgtagttt
    48361 ggtagggtga agagttgggt ctggatacta tgaaggagtt gctccatgct accagtcgga
    48421 aggtcggtcc gtccgatagt ttcgcggaat agagcatctc ccgtcaagac tagatgagca
    48481 tcaggaaaga ctagggaaac accaccgata gagtgccctg gggtcggtag aaccttaaaa
    48541 cgaaattcct ctagttggta ttcttcgtga aagacaaagg tgtgttctgc aggttttgtg
    48601 accacatctg ccatatcatc gtggcgaggg agaccggaga gattatcgac aggagtgtag
    48661 agccagctgg cttcgctctc tgcgatatag acaggaggat tgccaaacgt ctcgcgaacc
    48721 aagtccagac tcatgatatg gtcataatgg gcgtgggtca agagaatagc acagatcggt
    48781 ttgttgatct tctcgattgt ctgacgaatg gcttcccaat gactaccagg atcgacgacg
    48841 atgaggtgct tttcgccttc tagataatag gtattttcat aggcaacagg attcacggtt
    48901 ttatggattt tcatactagc tccaatctca aagaatgtac taatactctt cgaaaatctc
    48961 ttcaaaccgc gtcaacttta tctgcaacct caaagcagtg ctttgaacag cctgcggcta
    49021 gctttctagt ttgctctttg attttcattg agtataaatt aagtatagca cagttaggga
    49081 gaataggtaa ggatttaaaa tgaacttaaa aaccagcatg actagatgaa gacaggctgg
    49141 ttatcaattt atcaatattg gaaaggtgtc aattgccctt cataggttgc ataaactccg
    49201 tcattagctt gtttgataga cgtgatgttt agagtgctac cgtagttggc ttctcatttg
    49261 tcgctatatt cactataagt tatcctactg ggcaggttct ggtcgttagc atagtattga
    49321 acgactgttt tttatagctt tgctggctga ataggaacag gcagcaaact cctaacatta
    49381 ggagagcaaa gatagaaata gcagtttttt tcataggtat tctccttata aactaaagga
    49441 tagaagaaag tatcttgagg atttttatac tcttcaaaaa tctcttcaaa ccgcgtcaac
    49501 tttatctgca acctcaaagc ggtgctttga gtaacctgcg gctagtttcc tagtttgctc
    49561 tttgattttc atttagtatt aattccaaaa tccagtcaag gtaccagtgt atgtgatacg
    49621 agttccaccg tttatctttc gtatcttcaa tggaattggg gaagtcagaa agtctttggg
    49681 ttacaatatg gtttccgttc ttctatctct ccttctatca cctaagttta aaatctaact
    49741 tcgtagtata ttacggacca ataattcaga attatcaagc aattttgaat tatttttttc
    49801 ttattctgtt ccaatctagt tttcatattc ttaaaaaagt gataaaatgg taggaagaat
    49861 tggagagtag agatgccgaa agaagtgaat ttaacaggcg aagaagttgt cgctttaacc
    49921 aaagaatatt taacggaaga ggatgttcat tttgtccata aggccttggt ctatgctgtt
    49981 gaatgccaca gtggtcaata tcgcaaatca ggcgagcctt atatcattca ccctatccaa
    50041 gtggcaggta ttttagctaa gctaaagctg gatgctgtaa cagtagcttg tggattcttg
    50101 catgatgtgg tggaagatac agatgcgact ttggacgatt tggaaagaga gtttggtcct
    50161 gatgtgcgga tgattgttga cggagttacc aagcttggca aggtcgagta caaatcgatc
    50221 gaggagcaat tagcggaaaa tcatcgcaag atgctcatgg ccatgtctga ggacatccgc
    50281 gttattttgg tcaaactgtc tgaccgcttg cacaatatgc ggaccctgaa acatcttcga
    50341 aaagacaagc aggagcgtat ttccaaagaa accatggaaa tctatgcccc gcttgcccat
    50401 cgtttgggga tttccagtgt caaatgggaa ttagaagact tgtctttccg ttatctcaat
    50461 ccaacggagt tttacaagat tacccatatg atgaaggaaa agcgcaggga gcgtgaggcc
    50521 ttggtggatg aggtagtcac aaaattagag gagtatacga cagaacgtca cttgaaaggg
    50581 aagatttatg gtcgtcccaa gcatatttac tcaattttcc gcaaaatgca ggacaagaga
    50641 aaacggtttg aggaaatcta tgatctgatt gctattcgtt gtattttaga tacccaaagt
    50701 gatgtttatg ccatgcttgg ttacgtgcat gaattttgga aaccgatgcc aggtcgcttc
    50761 aaagactata tcgccaaccg caaggccaat ggttatcagt ctatccatac gactgtttat
    50821 ggaccaaaag ggccgattga attccagatt cgaaccaagg aaatgcacga ggtggctgag
    50881 tacggggttg cggctcactg ggcttataag aaaggtataa aggggcaagt taacagcaag
    50941 gaatcagcta ttggaatgaa ctggatcaag gagatgatgg agctccaaga ccaggctgat
    51001 gatgctaagg aatttgtgga ctctgttaag gaaaactatt tggctgagga gatttacgtt
    51061 tttaccccag atggagctgt ccgttccctt cccaaagatt caggaccgat tgattttgcc
    51121 tacgaaatcc ataccaaggt cggtgaaaaa gcaactggtg ccaaggtcaa tggccgcatg
    51181 gttccactga caaccaagtt aaagacaggg gatcaggttg aaattatcgc caacccgaac
    51241 tcctttggac ctagccgtga ctggctcaat atggtcaaga ctagcaaggc gcgcaataag
    51301 attcgccagt tctttaaaaa ccaagataag gaattgtctg tcaacaaggg tcgtgagatg
    51361 ctgatggctc agttccaaga aaatggctat gtggcaaata aatttatgga caagcgccac
    51421 atggatcaag ttctgcaaaa gaccagttac aagacagaag actccctctt tgcggccatt
    51481 ggttttgggg aaatcggtgc gattaccgtc tttaaccgtc tgactgaaaa ggaacgccgt
    51541 gaggaagagc gtgccaaggc caaagctgag gcagaggagc ttgtcaaagg tggcgaggtc
    51601 aaggttgaaa ataaagaaac cctcaaggtc aagcatgagg ggggagtggt tattgaaggt
    51661 gcttctggtc tcctagtgcg gattgctaag tgttgtaacc ccgtgcctgg tgacgatatt
    51721 gttggctaca ttaccaaggg tcgtggtgtg gctattcacc gtgtggactg tatgaacctg
    51781 cgtgcccaag aaaactacga gcaacgtctc cttgatgtgg aatgggaaga ccagtaccct
    51841 agctcaaata aggagtatat ggcccatatc gatatctacg gtctcaaccg tacaggactg
    51901 ttgaacgatg tactgcaagt tctttcaaat acaaccaaga atatttcaac ggtcaatgcc
    51961 caaccaacca aggatatgaa gtttgctaat atccatgtgt ccttcggtat tgccaacctc
    52021 tctacactga ccacggttgt cgataaaatt aagagtgtgc cagaagttta ctctgtcaaa
    52081 cggaccaacg gctagttgtc ctagctctta ctagaaaggc tattatgaaa atcattatcc
    52141 aacgggttaa aaaagcccaa gtgagtatag aaggccagat tcagggaaaa atcaatcagg
    52201 gacttttatt gctggttggt gttggaccag aggaccaaga ggaagatttg gactatgctg
    52261 tgagaaaact ggtcaatatg cggatttttt cagacgcaga aggcaagatg aacctgtctg
    52321 tcaaagatat tgaaggagaa atcctctcta tttctcagtt taccctcttt gcggatacta
    52381 agagaggcaa tcgtccagcc tttacagggg cagctaaacc tgatatggca tcagacttct
    52441 atgatgcttt caatcaaaaa ttagcgcaag aagtgcccgt tcagacaggc atctttggag
    52501 cagatatgca ggttgagctg gttaataatg gacctgttac cattatccta gatactaaaa
    52561 agagataaga aagaccaagc ccagtcggct tggtctttct catctatcat aaaatactcc
    52621 aaaaagaaat cggttcttga tatgcttggg ggaagtctct tttcaggctt tggcagatgc
    52681 gataggaagg gatgagatgt cctaggatga ggagagttcc ctgaaggaaa agtggaatat
    52741 cacttaaaga caccaaggag agaatcatca ggctatccca taggagaatt
//
LOCUS       DBDESG010000005        49987 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.005, whole genome shotgun sequence.
ACCESSION   DBDESG010000005 DBDESG010000000
VERSION     DBDESG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 49987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.005"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1068)
                     /locus_tag="ACKB38_000285"
     CDS             complement(<1..1068)
                     /locus_tag="ACKB38_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HGL7979132.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILL"
     gene            complement(1371..2084)
                     /gene="rsmG"
                     /locus_tag="ACKB38_000286"
     CDS             complement(1371..2084)
                     /gene="rsmG"
                     /locus_tag="ACKB38_000286"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGL7979133.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            2178..2738
                     /locus_tag="ACKB38_000287"
     CDS             2178..2738
                     /locus_tag="ACKB38_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGL7979134.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            2740..3639
                     /gene="htpX"
                     /locus_tag="ACKB38_000288"
     CDS             2740..3639
                     /gene="htpX"
                     /locus_tag="ACKB38_000288"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGL7979135.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(3683..5242)
                     /locus_tag="ACKB38_000289"
     CDS             complement(3683..5242)
                     /locus_tag="ACKB38_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGL7979136.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(5481..5798)
                     /locus_tag="ACKB38_000290"
     CDS             complement(5481..5798)
                     /locus_tag="ACKB38_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGL7979137.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
     gene            5968..6510
                     /locus_tag="ACKB38_000291"
     CDS             5968..6510
                     /locus_tag="ACKB38_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGL7979138.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            6510..7139
                     /gene="nth"
                     /locus_tag="ACKB38_000292"
     CDS             6510..7139
                     /gene="nth"
                     /locus_tag="ACKB38_000292"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGL7979139.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            7350..7871
                     /gene="pyrR"
                     /locus_tag="ACKB38_000293"
     CDS             7350..7871
                     /gene="pyrR"
                     /locus_tag="ACKB38_000293"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGL7979140.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            7890..8813
                     /locus_tag="ACKB38_000294"
     CDS             7890..8813
                     /locus_tag="ACKB38_000294"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGL7979141.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            8863..9942
                     /locus_tag="ACKB38_000295"
     CDS             8863..9942
                     /locus_tag="ACKB38_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGL7979142.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            10255..13431
                     /gene="carB"
                     /locus_tag="ACKB38_000296"
     CDS             10255..13431
                     /gene="carB"
                     /locus_tag="ACKB38_000296"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGL7979143.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(13706..14515)
                     /locus_tag="ACKB38_000297"
     CDS             complement(13706..14515)
                     /locus_tag="ACKB38_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGL7979144.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(14517..15320)
                     /locus_tag="ACKB38_000298"
     CDS             complement(14517..15320)
                     /locus_tag="ACKB38_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGL7979145.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(15330..16817)
                     /gene="tacF"
                     /locus_tag="ACKB38_000299"
     CDS             complement(15330..16817)
                     /gene="tacF"
                     /locus_tag="ACKB38_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGL7979146.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            17153..17860
                     /locus_tag="ACKB38_000300"
     CDS             17153..17860
                     /locus_tag="ACKB38_000300"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGL7979147.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            17865..18887
                     /locus_tag="ACKB38_000301"
     CDS             17865..18887
                     /locus_tag="ACKB38_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGL7979148.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            18904..19773
                     /gene="licA"
                     /locus_tag="ACKB38_000302"
     CDS             18904..19773
                     /gene="licA"
                     /locus_tag="ACKB38_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGL7979149.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            19763..20641
                     /locus_tag="ACKB38_000303"
     CDS             19763..20641
                     /locus_tag="ACKB38_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGL7979150.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            20653..21342
                     /locus_tag="ACKB38_000304"
     CDS             20653..21342
                     /locus_tag="ACKB38_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGL7979151.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            21438..22286
                     /gene="dprA"
                     /locus_tag="ACKB38_000305"
     CDS             21438..22286
                     /gene="dprA"
                     /locus_tag="ACKB38_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGL7979152.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(22470..22592)
                     /locus_tag="ACKB38_000306"
     CDS             complement(22470..22592)
                     /locus_tag="ACKB38_000306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979153.1"
                     /translation="MKIKAQTRKLATGCSKHCFEVVDKTDEVSSKYCFEVADGS"
     gene            23176..24219
                     /locus_tag="ACKB38_000307"
     CDS             23176..24219
                     /locus_tag="ACKB38_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGL7979154.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            24386..26473
                     /gene="topA"
                     /locus_tag="ACKB38_000308"
     CDS             24386..26473
                     /gene="topA"
                     /locus_tag="ACKB38_000308"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGL7979155.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            26694..27053
                     /locus_tag="ACKB38_000309"
     CDS             26694..27053
                     /locus_tag="ACKB38_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGL7979156.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            27146..27778
                     /locus_tag="ACKB38_000310"
     CDS             27146..27778
                     /locus_tag="ACKB38_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGL7979157.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQAEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            27822..28478
                     /locus_tag="ACKB38_000311"
     CDS             27822..28478
                     /locus_tag="ACKB38_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGL7979158.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            28548..29591
                     /locus_tag="ACKB38_000312"
                     /pseudo
     CDS             28548..29591
                     /locus_tag="ACKB38_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            29603..30640
                     /gene="leuB"
                     /locus_tag="ACKB38_000313"
                     /pseudo
     CDS             29603..30640
                     /gene="leuB"
                     /locus_tag="ACKB38_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            30637..30906
                     /locus_tag="ACKB38_000314"
     CDS             30637..30906
                     /locus_tag="ACKB38_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGL7979159.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            30910..31269
                     /gene="leuD"
                     /locus_tag="ACKB38_000315"
     CDS             30910..31269
                     /gene="leuD"
                     /locus_tag="ACKB38_000315"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGL7979160.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            31431..32053
                     /locus_tag="ACKB38_000316"
                     /pseudo
     CDS             31431..32053
                     /locus_tag="ACKB38_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899133.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            32217..34148
                     /locus_tag="ACKB38_000317"
     CDS             32217..34148
                     /locus_tag="ACKB38_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGL7979161.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKNFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTVEGQAGMLDKELNIHAEEAKIRLRNLNAAIENIQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFDLTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            34135..35451
                     /locus_tag="ACKB38_000318"
     CDS             34135..35451
                     /locus_tag="ACKB38_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGL7979162.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNPLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            35626..36612
                     /locus_tag="ACKB38_000319"
     CDS             35626..36612
                     /locus_tag="ACKB38_000319"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGL7979163.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVSQLKADTPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            36676..36747
                     /locus_tag="ACKB38_000320"
     tRNA            36676..36747
                     /locus_tag="ACKB38_000320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36709..36711,aa:Arg,seq:ccg)
     gene            36930..37628
                     /gene="rnc"
                     /locus_tag="ACKB38_000321"
     CDS             36930..37628
                     /gene="rnc"
                     /locus_tag="ACKB38_000321"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGL7979164.1"
                     /translation="MKELQTVLKNHFAIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            37619..41158
                     /gene="smc"
                     /locus_tag="ACKB38_000322"
     CDS             37619..41158
                     /gene="smc"
                     /locus_tag="ACKB38_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGL7979165.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HVYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAISSDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKIDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            41155..41949
                     /locus_tag="ACKB38_000323"
     CDS             41155..41949
                     /locus_tag="ACKB38_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGL7979166.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            41949..42767
                     /locus_tag="ACKB38_000324"
     CDS             41949..42767
                     /locus_tag="ACKB38_000324"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGL7979167.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            42771..44060
                     /gene="ftsY"
                     /locus_tag="ACKB38_000325"
     CDS             42771..44060
                     /gene="ftsY"
                     /locus_tag="ACKB38_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGL7979168.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(44106..45593)
                     /gene="zwf"
                     /locus_tag="ACKB38_000326"
     CDS             complement(44106..45593)
                     /gene="zwf"
                     /locus_tag="ACKB38_000326"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGL7979169.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(45742..46482)
                     /locus_tag="ACKB38_000327"
     CDS             complement(45742..46482)
                     /locus_tag="ACKB38_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7979170.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(46482..48647)
                     /locus_tag="ACKB38_000328"
     CDS             complement(46482..48647)
                     /locus_tag="ACKB38_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGL7979171.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            48817..49770
                     /locus_tag="ACKB38_000329"
     CDS             48817..49770
                     /locus_tag="ACKB38_000329"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGL7979172.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            49847..>49987
                     /locus_tag="ACKB38_000330"
     CDS             49847..>49987
                     /locus_tag="ACKB38_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal transporter CzcA"
                     /protein_id="HGL7979173.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATG"
BASE COUNT        15300 a         9015 c        10861 g        14811 t
ORIGIN      
        1 gagaaggatt gacataccac caagtacagc gtttggaata gttgaaatca aggcagtgaa
       61 tttaccaagg aagctgaggg caatcgcgat gaaggcagcg ttacggataa ctgagacaga
      121 agcgatacga gtcataccga taacccctgt attttctccg taagttgtat tggctggtcc
      181 accaaggaag gcagaaacag aagttgcgat accgtcacca agaagagtac ggtgaagacc
      241 tggttctttt aagaattgac gaccacagat ttgacccaaa acagtatggt ctccgatatg
      301 ttcagaaatt gttacgatag cgattggcaa gatagcgatg gcttctggac caaagtaaag
      361 attgtactct ttaaaggcac cacctgtgct aaatggcaag tagaaaccag gaatttcgaa
      421 ccagttggct ttaagaactg gtgtaaagtc aaccaagcca agagttagtg cgaaaaggta
      481 accaccgata atggcaaaga ggaatggaat gattcgtagg aagccttttc cttttgtatt
      541 gataaaggca gcaattagga aagtaacaac ggctaccaga gcatttttcc aatttccgtc
      601 tgctacaaga cctgcattgg taacagctga acctgcaagt ccaagaccga taacgatgat
      661 cataggaccg ataatgattg gtggcaagag tttatcaatc cattttgttc ctacaaatcg
      721 gatgctggta gcaacaagga catagaccaa accagtcaag ataacccctg tttgggcagc
      781 agatacatcc ccccccattt ctttcatagc cagtgacata gctgtgataa aggcaaatga
      841 agaacctaga taaactggaa ctttaaaacc agtagcaatc atgtagatga gtgttccaac
      901 acctgaagca aaaagggcaa cagatacagg cattcccaaa atcaatggta ccaagatggt
      961 cgcaccaaac atggcgaaaa cgtgttggaa actaaggaga attccttttc cagccgaagg
     1021 acgttggtca acgtctagta acaaatcaac agttgattct tgtttcatat aaacctcact
     1081 tttgggcatc aaaaaagagc ccattatttt acagcaatag gccctcagag taagacttct
     1141 ttaaaattta gattttaaag agttttaaat atttctgcgt cttcgtcacc tcacgggatg
     1201 acattaaaaa cctttgctta ggatagtata gcagaaaaaa atgattttgt aaatcatttt
     1261 ttcccgagcc tagaaataaa agagcgaggc tgattttgta aatcattttt tcccgagcct
     1321 agaaataaaa gagcgaggct gattttgtaa acatttgttt actaaaaaat ttaaagtggg
     1381 cgtttatttg gcataccagc cttacgtgga tatttatttg gtgtttcttt tttcttttct
     1441 accactgtga tatagcgcgg atctctattc ggtagggcgt agctgagatt gtcttcgacc
     1501 ttactaaaaa ggagattgag ggcattctta gcttctaata attcctcagg cgcattgcta
     1561 gccttgagtg ctaatagttt gccaccaacc ttaaggtagg gaatagtcaa ttcagatagg
     1621 acctgcatac gggcaaccgc acgagctgtt acaaaatcat attgagcacg gaagttcttg
     1681 tcttgggcaa aatcttcggc acgtccgtgg tagaaatgaa ctccgttcaa atccagttct
     1741 tgagccaaga gttgtaggaa gttgatgcgc ttattgagtg aatcaataat ggtcacatct
     1801 aactccggat agagaatttt cattggtaga ctaggaaatc ctgccccagc cccgatatca
     1861 agaagtttga tagtttcatt gggaatcaaa ccttgaagaa tgggtgcaat cgaatcgtaa
     1921 aaatgtttga gataaacttc ttccttgtcc gtaatcgccg tcaaattaat cttctcattc
     1981 cactcgacca agagctcaaa ataacgttca aattgttctt tttgctggtc cgaaagtgga
     2041 agattctgct cggcaagcaa gttgtaaaat gtttttggtt tcatagttat ttccttcttt
     2101 agtatcatct tattttacca caatcataga aaatttgata tactggtact aatctaaaag
     2161 cagaaaggac ttatattatg acttggatta ttcttggagt tatcgctctt attgttattt
     2221 ttgtgattgt tagctataac ggtttggtta aaaatcgtat gcaaaccaag gaggcttgga
     2281 gtcagattga tgttcagttg aaacgtcgca atgacctctt gccaaacttg attgagactg
     2341 taaaaggtta tgccaaatat gaaggttcta cccttgaaaa ggtggcagaa ctacgtaacc
     2401 aagtggcggc agcgacttca ccagcagaag ctatgaaagc cagtgatgcc ctcactcgtc
     2461 aggtttcagg tatttttgca gttgcagaaa gctatccaga tttgaaagct agtgctaact
     2521 ttgttaaatt gcaagaggag ttgacaaaca cagaaaataa aatttcttac tctcgtcaac
     2581 tctataacag tgttgtcagc aactacaatg taaaattaga aactttcccg agcaatatta
     2641 tcgctggaat gtttggattt aaagcggcag atttccttca aacacctgaa gaggaaaagt
     2701 cggttcctaa agttgatttt agcggtttag gtgactaaga tgttgtttga tcaaattgca
     2761 agcaataaac gaaaaacctg gattttgttg ctggtatttt tcctactctt agctcttgtt
     2821 ggttatgcgg ttggttatct ctttataaga tctggacttg gtggtttggt tattgcactg
     2881 attatcggct ttatctacgc tttgtctatg atttttcaat cgacagagat tgtcatgtcc
     2941 atgaatggag cgcgtgaggt ggatgagcaa acggcaccag acctctacca tgtagtggaa
     3001 gatatggctc tggtcgctca gattcctatg ccccgtgttt tcatcattga tgatccagcc
     3061 ttaaatgcct ttgcgacagg ttctaatcct caaaatgcgg ctgttgctgc gacttcaggt
     3121 ctactagcta tcatgaatcg tgaagaacta gaagctgtta tgggacatga agtcagtcat
     3181 attcgtaatt atgatatccg tatttcgact attgcagttg cccttgctag tgctatcacc
     3241 atgctttcta gtatggcagg tcgtatgatg tggtggggtg gagcaggtcg cagacgaagt
     3301 gatgatgacc gagatggaaa tggtcttgaa atcattatgc tagtggtttc cctactagct
     3361 attgtactgg cacctctcgc tgcaaccttg gttcagctcg ctatttctcg tcagagggaa
     3421 tttctggcag atgcatctag tgtcgagctg actcgcaatc cccagggaat gattaatgcc
     3481 ctagataagt tggacaatag caaacctatg agtcgccacg tcgatgatgc tagcagtgcc
     3541 ctttatatca atgatcctaa gaaaggtggg gggttccaaa aactctttta tacccaccca
     3601 cctatctcag aacggattga acgtttaaaa cagatgtaaa agaatccaga ggtcgtctct
     3661 ggatttttgc tatgttgcaa atttataaat cttctaaatt aactagcttc aaaccgtgtt
     3721 ctgtcatgcg atagatgatc gagcagactt cttttaagaa acgacggtca tgcgaaacag
     3781 tgatgagacc gcctggatag gtagcaaaga gttttctgat ttggggttga gaagtgggag
     3841 aaaagtttcg tgtgggttca tccagcagga gaaagtttgg tttgcgcagg actaaatcca
     3901 aaagcaggag ttttccctgt tgtccgccag ataaggagcg aatttgatgc tgcatttctg
     3961 gataactgaa attgagacta gctaggtgag attggatttt ctgtagttcc tctttttccc
     4021 cagttttact gagataggct attggggata aatccaattg cagttttttg tggtaatctt
     4081 gtggcataaa accaagtgaa atctctcttt tatcattcag aagtctctgt aacttggcta
     4141 acagagttga tttcccaaca ccatttggcc cgataatacc gattttttct tggccacgga
     4201 cagttagttg tagtttttga accaaaactc ggtcgtcaat ggacaaattt tctttttcca
     4261 gttggactaa gactttagaa gctggtaatg gttggatgtc tgaaaagaaa agttggattt
     4321 gttcctcttc aagtggcttt tgagtcatgg actgagctgc cttttcgtag cgtttttctt
     4381 gtgagaggac agttttcatc tttttagcca ataggcgacc ggcagtacta tctttggtag
     4441 ctcgaagcgc agtttctaca ttttgcttaa ctctccgatg tttttccatg gttttatcgt
     4501 aggctctttg gttgttagca gcttgctgac tttgtttggc aaaattagcc tttctctgct
     4561 cactatagct atcatagtct aaatgctcta ctagcgtttc cgcttcttta cggtgtttga
     4621 ccagtcgcaa gtgaacaata gtgtctgccg tttcagaaag aaagtcttca tcatgggaaa
     4681 tgaaaataac ggtttgcctg gtcttttgaa tctggccttt tagccaatca actgtctcaa
     4741 ggtctaggtc atttgaaggt tcatctaaaa atagaatctc aaagggtttg gctaactcat
     4801 ggataagctg aattttcaaa gcttcgcccc ctgatagatt gccaatctct tggtcacttg
     4861 cgaaacgatt gctatcaaaa tgcaattcct ccgccaaacg atagaggata ctgtagtcta
     4921 aatcaataga atctaaaaag aagtagtcgt gtaaagtttt cttttttagg tcctcaggga
     4981 ctttttgagg aatgtaggcc agtgactgat agtcagattg gatgtttccc ttgatagtga
     5041 aatcagacaa agcttccccc attaaaattt taagtaaggt tgatttgcca tttccttctt
     5101 caccaataat agcaaccttt tccccgtctt ggatggtcat ggttaggtca gatacaagat
     5161 ctcgtaaatc tttgttttgt gtgatggtta gatgattgat ttttatcata tgatttccct
     5221 ttctagaatg ttcatagtgc ataacaaaaa gctctacttt acctagtaga gcccaaagtc
     5281 tatgtcaaaa aaactctatt aacatgccag aaaaagagca taaaagagca cacaaaaaga
     5341 gacaaaaaca agatctacta aataaaggtt ctttatttga tagacttagt tgttcatgtc
     5401 tcccttacct ccgagtatta gtatctttat attatacctt taaggaaatt ttgtcaacag
     5461 aaaactggaa attctatgct ctaaaattca aaaacaggtc catagagact tagtacgtgt
     5521 ttgacatgat cgtaatggaa tttatcatag tttcgccaag cagtgcgtgc ttgatcgttt
     5581 agttttaggc tacccagtcc aatcagaaga ctggtaggtt ggtaaaattt ctcaagatca
     5641 attaagatac tgttgttcaa ctgttccgtt tttttgagct cttgcatagt agtgtttagc
     5701 agctcttgta gtcgaatatc atctgccgta ccctgtttac agttttggta gcttttattt
     5761 aacttggata ggagttgaat gagtttcttg tcttccatgt gagcatcctc cttgatctga
     5821 tgtactcttt ccctatagta tagcactaaa aaataaaact atcatagtaa atatgttaaa
     5881 attttgaatt ttaattacaa agtttggtgt gctgatttgt agccttttca tggtataatc
     5941 aagagagtaa atctttatgg aggaagtatg aaattaaata ttcaagaaat tcgtaagcag
     6001 tctgaaggtt tgaactttga acaaacgtta gatttagttg atgacctgcg tgcacgtaat
     6061 caagaaattt tagatgtaaa agatatcctt gcagttggga aagtacaata tgaagaccgt
     6121 atgtatttct tagattatca actatcttat accattgttc ttgcttcgag tcgcagtatg
     6181 gagccagttg agttagttga atcttatcca gtcacggaag ttttcatgga aggcgcaact
     6241 aaccagctag atcaagaagt tttagatgat gacttggtct tgcccatcga aaatggggag
     6301 cttgaccttg ctgagagtgt atcagacaat atcctgctaa acattcctat caaggtcttg
     6361 acggctgaag aagaagctgg tcaaggattt atctcaggaa atgactggca aatcatgaca
     6421 gaggaagaat accaagctca aaaagcagta aagaaagaag aaaacagtcc ttttgctggc
     6481 ttacaaggac tatttgacgg agatgaataa tggtcttgtc aaaaaaacga gcacgaaagg
     6541 tgctagaaga aatcattgcc ctcttcccag atgccaagcc tagtcttgat tttaccaatc
     6601 attttgaact cctggttgcg gtcatgttat cagcccagac gacagatgca gcggtaaata
     6661 aggccacacc aggtctcttt gttgcctttc caacacccca agctatgtct gtagcgacag
     6721 agagtgagat tgcttcacat atttctcgct tgggattgta tcggaataaa gctaaattcc
     6781 ttaaaaaatg tgcccaacag ttactagacg attttgatgg tcaagtccct cagacacgtg
     6841 aagaattgga gagtttggca ggtgttggtc gcaagacagc caatgttgtc atgagtgtag
     6901 gatttggaat tccagccttt gcagtggata ctcatgtgga gcgtatttgc aaacaccacg
     6961 atattgtcaa aaaatcagcg acgccacttg aggtggaaaa gcgggtcatg gatatcttgc
     7021 cgcctgagca gtggttagct gcccatcagg ccatgattta ttttggaaga gccatttgcc
     7081 atccaaaaaa tccagagtgt gaccagtacc cacaattata tgattttagc aatttgtaag
     7141 atgctacatt gaaacttttt gcttgatttt aagcatgctt tgtgctatag taaatgataa
     7201 ctaaatattc ctttaaacct gtcccgtgag gcaggcaagg agcgcgataa agtagaaggg
     7261 tgaagtctat actgtgtgca gtagggctcg cttggctata aatttcaagt ctccttgttt
     7321 aaggagactt ttctttttgg aggtttgtca tgaaaacaaa agaagttgta gacgaattga
     7381 ccgtcaaacg agcgattacg cgtattactt atgagattat cgaacgcaac aaagatttga
     7441 ataagatcgt cttggctggt attaaaactc gtggtgtctt tattgcccac cgaatccaag
     7501 aacgtttgaa gcagttagaa aatctttcag ttcctgttgt ggaattggat actaaacctt
     7561 tccgtgatga tgttaaaagt ggagaagata cttctttggt ttctgtcgat gtgacagacc
     7621 gcgaagttat cttggtggat gatgtgctct atacaggtcg taccatccgt gctgctattg
     7681 ataatattgt cggtcatggt cgtcctgcgc gtgtgagttt agcagttcta gtcgatcgtg
     7741 gacatagaga attgccaatc cgtccagatt acgttggaaa aaatatccca accagtcgtt
     7801 ctgaagaaat catcgtagag atgacagaac ttgatgacca agacagagtt ctgattactg
     7861 aagaagctta gaaagttaaa ggagtagcca tgtcagaaaa tcaacaagca ttgaaccatg
     7921 tggtgtccat ggaagacctc actgtcgatc aagtaatgaa attgatcaag cgaggaattg
     7981 agtttaaaaa tggagcccag cttccctatg aagaccatcc gattgtttcc aatctcttct
     8041 ttgaggattc tacacggaca cataagtcct ttgaagtcgc agagattaaa cttggattgg
     8101 aacgacttga ctttgatgtg aagactagct cggttaataa gggtgagaca ctttatgaca
     8161 ccattttgac tctgtctgct ttaggagtgg atgtctgtgt gattcgccat ccagaggtcg
     8221 actactatag agaattgatt gctagtccaa cgattacgac ttccatcatc aatggtggag
     8281 atggttcggg ccaacaccct agccagagct tgcttgattt gatgaccatt tatgaggaat
     8341 ttggccactt tgagggtctt aaagttgcta ttgcaggtga cttggaccac tcacgcgttg
     8401 ccaaatccaa tatgcagatt ttgaaacgct tgggagctga actctttttc gctggacctg
     8461 aggaatggag aagtcaagag tttgcagact atggacagtt tgtaaccatt gatgaaatca
     8521 ttgatcaggt tgatgtcatg atgtttctcc gtgtgcaaca cgaacgccat gatagtggag
     8581 ctgtattttc aaaagaagac taccatgccc aacatggctt gactcaagaa cgttacgatc
     8641 gtttgaaaga aacagcaatc ctcatgcacc cagctccaat caatcgtgat gtagaaatag
     8701 cagaccactt ggttgaagca ccaaaatcac ggattgtcca acaaatgacc aatggtgtct
     8761 ttgttcgaat ggcaatctta gaatccgtac tagcgagtag aaacgccaac taaaacacaa
     8821 gacgttaaaa gacgccagtg agcgtccacg agaggtgaaa atatgacaaa aagaattcta
     8881 gtattagaag atggcacagt ttttgaaggc aaggccttcg gagcagatat tgatgtaaca
     8941 ggcgaaatcg tctttaatac agggatgacc ggctaccaag aatccattac agaccagtct
     9001 tataatggac aaatcttgac ctttacttat cctttggtag gaaattatgg tattaatcgt
     9061 gatgattatg aatccattat tccaacttgt aagggagtcg ttgtttttga agaagcgcgt
     9121 agagctagca actggcgcaa ccaaatgacc ttggatgaat ttttgaaagc caagaaaatt
     9181 ccagggattt caggaattga tacgcgtgct cttaccaaga ttatccgtaa gcatggtact
     9241 atgcgtgcaa ccttgaccca tgttggggac agtatggacc atgtgacgga ccagctccaa
     9301 gcaacagttt taccgacaga caatatcaaa caggtttcta ctaaaacgtc ttatccagct
     9361 ccaggagttg gtttgagcgt ggtgctagtg gactttggtc tcaagcactc aatcctacgt
     9421 gaactttcta aacgcaactg taacgtgaca gttgttcctt attcgacaac ggctgaagaa
     9481 attctccatc tcaatcctga cggagttatg ttgtcaaatg gtccaggtaa cccagaagac
     9541 gttccacaag cactcgacat gattcgcggt gtacaaggaa aaattccaat ctttggtatt
     9601 tgtatgggac accaactctt tgcaatggca aacggggcta agacctataa gatgaagttt
     9661 ggccaccgtg gattcaacca tgcggtacgt gaaatcgcaa caggacgagt agacttcacc
     9721 agccagaacc atggttatgc agtcagccgc gaggatttgc cagagcactt gattattacc
     9781 cacgaagaaa tcaatgacaa gtcagtggaa ggtgtgcgcc acagatacca acctgctttc
     9841 tctgttcaat accacccaga tgcagctcct gggccacacg acgctagcta cctatttgac
     9901 gagtttatcg agatgatgga agtttttaaa caatcaaact aagaacgagt aaaagctcgt
     9961 cggagaattt atgtaactga acacggacgg aaagctcaga atgtagagaa accaacttgg
    10021 attgtcaatc cttccttgat ttcctaaaat tcaatcgctt tctattcgtc ctttgtatct
    10081 tatttaatgt cgccctgaga ggcgacgaat agaaaggaga agggtgctcc gtatttgctg
    10141 aactgaatac gggctacgga cggtgctaaa aagatagtta ttcctagaac tgacagttct
    10201 tcgtcataac tcctatttta gctttgtccg cttgacaccc ttaatatctt atatatgcct
    10261 aaacgtactg atattcaaaa aattatggtg attggttctg gtccgattat tattggtcag
    10321 gctgctgagt ttgactacgc tgggactcag gcttgcttgt cgttgaaaga ggaaggttat
    10381 gaggttgttt tggttaactc aaatcctgca accatcatga cggacaagga aattgctgac
    10441 aaggtttaca ttgaaccgat tacacttgag tttgtgacac gtattcttcg taaggaaggt
    10501 ccagatgcct tgctaccaac actcggtggt cagacaggtc tcaacatggc catggaatta
    10561 tctaaaaatg gtatcctaga cgagcttggc gttgaacttc tgggtaccaa attatctgcc
    10621 attgaccaag cggaggaccg tgacctcttt aaacaattga tggaagagct tgaacaaccc
    10681 attccagaat ctgaaattgt taacacagta gaagaagcag ttgcctttgc agcgacaatt
    10741 ggctacccag tcatcgttcg tccagccttt acccttggtg gtactggtgg tggtatgtgt
    10801 gccaacgaga aagaattgcg tgaaatcact gaaaatggtt tgaaattgtc acctgttacc
    10861 caatgtttga ttgagcgttc gattgccggt ttcaaggaaa ttgaatacga agtgatgcgt
    10921 gactcagctg acaatgcctt ggttgtttgt aacatggaaa actttgaccc agttgggatt
    10981 cacacagggg attccattgt atttgcccct gcgcaaacca tgtcagacta tgaaaaccaa
    11041 atgcttcgtg atgcgagctt gagcattatt cgtgccctca agattgaagg tggatgtaat
    11101 gttcagctag ctcttgatcc taacagcttc aagtattatg ttatcgaagt aaaccctcgt
    11161 gtatcacgtt cgtcagccct tgcttctaaa gcgacaggat atccgattgc taagttggct
    11221 gccaagattg cagtaggttt gaccttggat gaggttataa acccagttac aggttcaacc
    11281 tatgccatgt ttgaaccagc ccttgactac gtagttgcta agattccacg tttcccattt
    11341 gacaagtttg aaaagggtga acgccgtctc ggtacacaaa tgaaggcgac tggagaagtc
    11401 atggcaatcg gtcgtaacat cgaggaatct cttctcaagg cctgccgctc ccttgaaatt
    11461 ggggtgcacc acaatgaaat acctgaactt gcagcagttt ctgatgatgc cttgattgaa
    11521 aaggttgtca aagcccaaga tgaccgtctc ttctacgtat cagaagccat tcgtcgtggc
    11581 tacacacctg aagaaattgc agagcttaca aaaattgata tcttctatct tgataaactc
    11641 ttgcatatct ttgaaattga gcaagaattg ggtgctcatc cacaagattt agaagttttg
    11701 aaaacagcca aacttaatgg tttttcagac cgtaagattg ctgaactctg gggaacgact
    11761 gatgataaag ttcgccaact tcgcttggaa aacaagattg ttccagttta taagatggta
    11821 gacacttgtg cggcagagtt cgactccgaa acgccatatt tctattcaac ctatggttgg
    11881 gaaaatgaat ctatcagatc tgataaagaa tctgtacttg tcctaggttc aggtccaatc
    11941 cgtatcggtc aaggggttga gtttgactac gcaaccgttc actctgttaa ggctatccag
    12001 gcagctggtt atgaagctat catcatgaac tcaaacccag agaccgtttc aacagacttc
    12061 tctgtatcag ataagcttta ctttgagcca ttgacattcg aagatgttat gaacgtcatt
    12121 gacttggaac aaccaaaggg agttatcgtt cagttcggtg gtcaaacagc catcaacctt
    12181 gcggagccat tggcaaaagc aggtgtgacc atccttggta cacaggttgc tgacctagat
    12241 cgtgccgaag accgtgacct cttcgagcaa gctctcaaag aattggatat tccacagcca
    12301 ccaggacaaa cggctaccaa tgaagaagaa gcagctcttg cagctcgcaa gattggcttc
    12361 ccagttcttg tccgcccatc ttatgtactt ggtggacgtg ccatggaaat cgttgaaaac
    12421 gaagaagacc ttcgttctta catgcgaact gctgttaagg ctagtccaga ccacccagtt
    12481 cttgtcgact cttatatcgt tgggcaagag tgcgaagttg atgccatttc agatggtaaa
    12541 aatgtactca ttcctggtat catggagcat atcgaacgtg ctggtgtcca ctcaggtgac
    12601 tcaatggctg tttacccacc acaaaccttg tcgcaaaagg ttcaggagac catcgcagac
    12661 tacaccaaac gcttagcaat cggtcttcac tgccttggaa tgatgaacat ccagtttgtt
    12721 atcaaggatg aaaaagtcta cgttattgag gtcaatccac gtgccagccg tactgttcca
    12781 ttcctatcta aggtaaccaa tattcctatg gctcaagtag cgaccaaact cattcttggt
    12841 caaagtttat cagaacttgg ctaccaaaat ggactttacc ctgaaagcac ccgtgttcat
    12901 atcaaggcac ctgtcttctc ctttaccaaa ctagctaagg tagacagctt gctcggtcct
    12961 gaaatgaagt caacaggtga agttatgggt tctgatgcta ctttggaaaa agctctctat
    13021 aaagcctttg aagcttctta tctacacttg ccaacttttg gtaatgtcgt ctttaccatc
    13081 gctgatgatg ccaaagaaga agccttgaac ttggctcgtc gtttccaaaa cattggttat
    13141 ggaatccttg cgacagaagg gacagcagcc ttctttgcta gtcatggctt gcaagcccaa
    13201 cctgttggta agattggtga tgacgataag gatatcccaa gttttgtacg caaaggaaga
    13261 attcaagcta tcattaacac agtaggaaca aaacgaactg ctgacgaaga tggtgagcaa
    13321 attcgtcgtt cagccattga acacggggtg cccctcttca cagcgctaga tacagctaat
    13381 gccatgctta aggtacttga aagtcgtagc tttgttacag aagcgattta aatagaaaaa
    13441 taagatatag cagactgaat ttagaacagt acacaggaat actaaaatat ttctataaat
    13501 taatttgaat tttctaattg atttgttcgc atcttatttc aatctactat agaaaaaagt
    13561 ctttaaaatt ataaaacgca tcatatcaag gtttttcaaa aaccttgata tgatgcgttt
    13621 tattgtggga atatgtattt cattttctac taaaattatg tttttgaata acctctatct
    13681 tagtagtttg tataatcccc ctcaatcagt ttttgcgata agctttaatg ctatgactat
    13741 accactcttg catttctttt gatggtggtg tcatataatc gccatacatc tgggttaaaa
    13801 attggtcata ttttttggga acaggcaaca tacggccttc aaactcagtt aaaatcagtt
    13861 ctttaaaggt atcaactggg aagatttctt tcatcccttc cttaccgatc ccaactcctc
    13921 cttcatattg aggagtgttg gttacagcat ttttgactag ttgatcaatt ttcttgtaaa
    13981 agtagcgagg attgacaaat cggagagcgt accagctaca taatctaaga aaatctttta
    14041 gtttgctatc accgtgaact gctcgtgatt ttttgatata agctagttga cgaagagcca
    14101 catacttata gctcttgtcg acaatgctca agtctgtaaa tcgatcaatt gggaagacat
    14161 cgatgaaaag gctggtatca tgacgcttgt acttaacatg gtcttctata acagtagaag
    14221 tgtccaaaat cgatgcgaaa ttatggaagt accaagaaga tgtatcgtag gaaagaacct
    14281 tgtagcgagg gtgattttct tcttcaataa tcttcagtaa acgctcataa tcctcacgat
    14341 aaagggaaat atcaatatca tcatcccaag gaatcatacc tttgtggcgg atggctccaa
    14401 gcatggttcc ataactgaga aaataaggaa tatcatgttt cttacaagtc tcatcaatat
    14461 agtccagcag ggctagttga atttctttaa tttctttttt ttctaaatat tgcatcctaa
    14521 tcctccaatt tataagcgtg aaattcatga ctgtagaagc gtttttcttc tggtggtagg
    14581 gtcatataat ctccataaaa ttgtgtcaaa atagtatcaa atttttcagg tgcaggaagg
    14641 cttaaattct caaagggtaa atcgattgtt ttatcaaagg taccacttgg gaagacttcc
    14701 ttttccttaa attttgaagg gataaaagcc atatattgcc cattttcacg actatatttt
    14761 tgaatttctt tctcgatttt atttgcaaaa taacgaggag aaaccggtcg aaggagtaac
    14821 cagaaggctg ttcgtatcca atcttttaaa aggctatcct tatagacaat atttttatgt
    14881 ttactgaaag acagcagttt gaagctttcc agtttataac aagtatcaat gaccttagga
    14941 tcatcaaagc gatctatagg gaaaatatcg ataaagatac cagactcata ggtttttgta
    15001 tttcgagtat caataatttt agtcgtactg tcggttatct tgataaagtt gttaaagtag
    15061 ttcttatcag tttctaagga taggagctta tacttgcttt tttccttttg aaaaatgtta
    15121 ataaatcgtt ggtagtcttc tctaggcatg gacagatcaa tatcgtcgtc ccaagggata
    15181 aagccctcat gtcgaaccgc cccaatcaga gtaccgtagt taataatata gttgatattg
    15241 tgctttttac agagagtatc aatataatcc aaaatttcta attcaatttg tttggcatct
    15301 tcaacggtta gttgtttcat tttaaactcc tatgattttt taaatttatt ttttaaggct
    15361 aggacatggt ttaaaaattc atagaaaatg ctatcttttg tgaagacaag tagactaata
    15421 taagagatag ctgatagcaa gacaatcaaa ccagtattaa tcaaaaatgg caaattaatg
    15481 accatatcta caggatacac gaaattaatc aggaaataaa ttgctacaaa ggaaagtgaa
    15541 aagagagagt atcgaacagt atagctaaag atatgtccca agtggatgag ttgttttcta
    15601 tggatgaaaa tgatatagaa aacaagtaga gaagtctctg atagcatagt tgtcagtaag
    15661 tagtattcag gagccacgat atggttgaaa aaaaggagac tattcaagcc caaattgagt
    15721 agcccagcaa agactgtata gactgtgata cgtttttcat agccattggt aaagagaatt
    15781 tgggaaccaa gaatggtatc taaggccagg ataatcgtac gaaaagcgaa gagagaggtc
    15841 aagatgccgc ctccgatata tttttcacta ccgtaaagta ggatggcatt tggtcctaaa
    15901 accatgagtc caaaactcag tggaatgata aagaagttaa agattcgact acctctatta
    15961 accagagaaa cataggcttc tttgtctcct ttccccagat agtaactgag acgaggcaca
    16021 ctcactccaa ttgcacctgt tacaacccca gctataacgg tcacaattcg ctgagctatg
    16081 gtatagtaac taacgttgac atcaatccct gttttaacga ggaagaggcg atctaaaaaa
    16141 gtgaagagca tattggcatt ggcaaagact aacatggctg tcagagggag aaagagtggt
    16201 ttaaaatcac ttaggtgaat tttaacaagt ttgatgtctc ttttaatcca aaaataacta
    16261 atcaggtagt taatcagcgt cgataaactc atcacaagtg tatagacaac aatatcgtgt
    16321 tcatttttaa caaataagaa aatagagacc agcatcagga tacggatgaa ggcagttttg
    16381 taaaagagaa aactgtaatt ttccagagct tcattgaccc attcgattga aaaaatctgg
    16441 gcaatgagtt gaatccccat aacaaggtag acctttttga cgattggatt atcagtaaag
    16501 aagagaggat aggctaggat atagacagca gtggtcaaaa tcgtacaagc gatgcacaaa
    16561 taaaaaagac tagaaaaggt tctgttaaga tcttttttgt tatccttgac attactgata
    16621 gcccttaaac cgtagttata gacaccataa gttgcaaagg gcaagaaaaa tgacaaaata
    16681 gtgtcgactg agttgaagta accatagtca gttcggtcca agacacgcgc gacataggtt
    16741 ccagttagga tgggaaaaat aatattcaag acacgaattc ccatgtaaga tagagcattt
    16801 aattttatac ttttcattca atttacctcg tttttcatta tatcataaag ttagctaata
    16861 agaaatgaag ggcagtaagt caagtaatca ctttgaagtt tcaaatctta agttttaagt
    16921 tttctttaag gaaagtatat tattctgaag gactctaaaa tttcgcagcc atttattagt
    16981 aattgctaca gaattcctag tcattactag aaatggacta gtttctttga ataatagaac
    17041 tgcataattc tcctattcta gaaggggagg accagtattt cttttatgat aggactagat
    17101 tgtggtataa tagagagaat aagttttttt agtaagacaa aggagaaaat agatgattta
    17161 tgcaggaatt cttgccggtg gaactggcac acgcatgggg atcagtaact tgccaaaaca
    17221 atttttagag ctaggtgatc gacctatttt gattcataca attgaaaaat ttgtcttgga
    17281 gccaagtatt gaaaaaattg tagttggtgt tcatggagac tgggtttctc atgcagaaga
    17341 tcttgtagat aaatatcttc ctctttataa ggaacgtatc atcattacaa agggtggtgc
    17401 tgaccgcaat acaagtatta agaacatcat tgaagccatt gatgcttatc gtccgcttac
    17461 tccagaggat atcgttgtta cccacgattc tgttcgtcca tttattacac ttcgcatgat
    17521 tcaggacaat atccaacttg cccaaaatca tgacgcagtg gacacagtgg tagaagcggt
    17581 tgatactatc gttgaaagta ccaatggtca atttattaca gatattccaa atcgtgctca
    17641 cctttatcaa ggacaaacac ctcaaacatt ccgttgcaag gacttcatgg acctttatgg
    17701 atctctttct gatgaagaga aggaaatctt gacagatgca tgtaaaatct ttgtgatcaa
    17761 aggaaaagat gtggctttgg ccaaaggtga atactcaaat ctgaagatta caaccgtaac
    17821 agatttgaag attgcaaaaa gtatgattga gaaagactag taaaatgatt aatcaaattt
    17881 atcaactaac taagcctaag tttatcaatg tcaaatatca ggaagaggct attgaccaag
    17941 agaatcatat ccttatccgt cccaactaca tggctgtctg tcatgcggat cagcgttact
    18001 atcagggaaa acgtgatccc aagattttga ataaaaagct tccaatggca atgattcacg
    18061 agtcatgtgg aaccgtcatt tctgacccga ccggaaccta cgaggttggt caaaaagttg
    18121 tcatgattcc caatcagtct cctatgcaga gtgatgaaga attctatgaa aactacatga
    18181 cagggaccca tttcttgtct agtggatttg atggctttat gagagagttt gtttctctcc
    18241 ctaaagatcg tgtggtggct tatgatgcta ttgaagatac ggttgcagcc attacagagt
    18301 ttgtcagtgt gggcatgcac gctatgaatc gtctattgac tcttgctcat agcaagcggg
    18361 agcggatcgc cgttattgga gatggaagtt tagcttttgt ggttgccaat attatcaact
    18421 atactttgcc agaagcagag attgtggtta ttggtcgtca ttgggaaaag ttggaactct
    18481 tctcatttgc caaagaatgc tatattacgg ataatattcc tgaagatttg gcctttgacc
    18541 atgcttttga atgttgtggt ggtgatggta ctggaccagc tattaatgac ttgattcgct
    18601 acattcgtcc tcagggaacg attctcatga tgggagttag cgaatataaa gtcaatctca
    18661 atactcgcga tgccttagaa aagggcttga ttttggttgg gtcatctcgt tctggtcgca
    18721 ttgattttga aaatgctatc caaatgatgg aagtcaagaa atttgccaat cgtcttaaaa
    18781 atatccttta tctagaagaa cctgtaagag aaattaaaga tattcatcgt gtctttgcaa
    18841 ccgatttaaa cacagccttt aaaacagtgt ttaagtggga agtataagta ctggaggtta
    18901 attgtggaga aaatcattaa agaaaaaatt tcttccttac ttagtcaaga agaggaagtc
    18961 ctcagtgttg aacaactggg tggaatgacc aatcaaaact atttggccaa aacaacaaat
    19021 aagcaataca ttgttaaatt ctttggtaaa gggacagaaa agcttatcaa tcgacaagat
    19081 gaaaagtaca atcttgaact actaaaggat ttaggcttag atgtaaaaaa ttatcttttt
    19141 gatattgaag ctggtatcaa agtaaatgag tatatcgaat ctgcgattac gcttgattca
    19201 acgtcaatca agaccaagtt cgacaaaatt actccaatat tacaaactat tcatacgtct
    19261 gctaaggaat taagaggaga atttgctcct tttgaagaaa tcaaaaaata cgaatccttg
    19321 attgaagaac aaattcctta tgccaactat gaatctgtta gaaatgcagt cttctcctta
    19381 gagaaaagac tggctgactt aggtgttgac agaaaatctt gtcatatcga tttggtgcct
    19441 gaaaacttta tcgaatcacc tcaaggacga ctttatttga ttgactggga atattcatca
    19501 atgaatgatc caatgtggga tttggctgcc ctctttttag agtctgaatt cacttcccaa
    19561 gaggaagaaa ctttcttatc tcactatgag agtgaccaaa caccggtttc tcatgaaaag
    19621 attgctattt ataaaatttt acaagatact atttggagtc tatggactgt ctataaggaa
    19681 gagcaaggtg aagattttgg tgactatggt gtgaatcgtt accaaagagc tattaaaggt
    19741 ttggcttctt atggaggttc agatgaaaag taaaaacgga gttccttttg gccttctctc
    19801 aggtattttc tggggcttgg gtctaacggt tagtgcttat atcttttcga tttttacaga
    19861 tttgtcaccc tttgtggtgg ctgcaactca tgattttttg agcatcttta tcttactagc
    19921 ttttctcttg gtaaaagaag ggaaagttcg cctctcaatt ttcttaaata ttcgcaatgt
    19981 cagtgttatc atcggagcct tgctagcagg ccctatcggt atgcaggcca atctttatgc
    20041 agttaagtat atcggaagtt ctttagcttc atctgtatcg gctatttacc ctgcgatttc
    20101 agttctattg gctttcttct ttttgaagca caagatttcg aaaaatactg tatttgggat
    20161 tgtcttgatt attggaggga ttattgctca gacctataag gttgaacagg ttaattcttt
    20221 ctacattggg attctttgtg ctttggtttg tgctattgca tggggaagtg agagtgttct
    20281 tagctctttt gccatggaaa gtgaattgag tgaaatcgaa gccctcttaa tccgtcaagt
    20341 aacttcgttc ttgtcctatc ttgtgattgt gctcttctct catcagtcat ttactgcagt
    20401 agccaatgga caattgctag gtctcatgat tgtttttgca gcctttgata tgatttccta
    20461 cttggcttat tatatcgcta tcaatcgctt gcaaccagcc aaggctacag gcttgaacgt
    20521 gagctatgta gtatggacgg tcttgtttgc agttgttttc ttgggtgcac cgctagatat
    20581 gctgaccatt atgacgtcac ttgtcgtcat tgctggagtt tatattatta ttaaagaata
    20641 aaggagattc gtgtgaaagc cattatctta gcagcgggat tgggaactcg cttgcgtcct
    20701 atgactgaaa atacccctaa agccttggtt caggttaatc aaaaaccttt gattgagtac
    20761 caaattgagt ttctcaaaga aaaaggaatc aatgacatca tcatcattgt tggttatctt
    20821 aaagaacaat tcgattactt gaaagagaaa tacggtgttc gtctcgtttt caatgataaa
    20881 tacgctgact acaataactt ttactctctc tatcttgtaa aagaagaatt ggccaacagc
    20941 tatgttattg atgctgacaa ttatctcttt aaaaatatgt tccgcaatga tttgacacgt
    21001 tcgacttatt ttagtgttta tcgtgaagat tgtaccaacg aatggttctt ggtttatgga
    21061 gatgactaca aggttcaaga cattattgtt gatagcaagg caggtcgcat ccttagtggt
    21121 gtatccttct gggatgctcc aactgcagaa aagattgtca gctttatcga caaggcttat
    21181 gtaagtggtg aatttgttga tctctattgg gacaatatgg ttaaggataa tatcaaagag
    21241 ctagatgtct atgttgaaga attagaaggc aatagcattt atgagatcga tagtgtccaa
    21301 gactatcgta aattagaaga aattcttaaa aacgaaaatt aaagattcca acatctgaca
    21361 aaatagtcgg atgttttttg attttttacg aacttttacg aatagataga tgagtagaaa
    21421 aagaaatgga gttatttatg aaaatcacaa actatgaaat ctataagtta aaaaaatcag
    21481 gtttgaccaa tcaacagatt ttgaaagtgc tagaatacgg tgaaaatgtt gatcaggagc
    21541 ttttgttggg tgatattgca gatatctcag gttgccgtaa tccagccgtt tttatggaac
    21601 gttattttca gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt ccatctttct
    21661 ctattttaga tgactgttat ccttgggatt tgagtgaaat atatgatgcg cctgtacttt
    21721 tattttacaa gggaaatctt gacctcctga aattcccgaa ggtagcggtc gtgggcagtc
    21781 gtgcttgtag caaacaggga gctaagtcag ttgaaaaagt cattcaaggc ttggaaaatg
    21841 aactggttat tgtcagtggt ctggccaagg gcattgacac agcagctcat atggcagctc
    21901 ttcagaatgg cggaaaaacc attgcagtga ttggaacagg actggatgtg ttttatccta
    21961 aagccaataa acgcttgcaa gactacatcg gcaatgacca tctggttcta agtgaatatg
    22021 gacctggtga acaacctctg aaatttcatt ttcctgcccg taatcgcatc attgctggac
    22081 tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc aggtagtctc attacgtgtg
    22141 agcgagcaat ggaagaagga cgcgatgtct ttgctattcc tggtagcatt ttagatggac
    22201 tatcagacgg ttgccatcat ttgattcaag aaggagcaaa attggtcacc agtgggcaag
    22261 atgttcttgc ggaatttgaa ttttaaaaat gacctaagct agaattctaa gaaaaaatca
    22321 attttaagag aaaatgaacc caacatttcc ataataaaac gcatattagc aagtttttaa
    22381 cacttgataa tatgcgtttt ttctaagtgg attagtagag tagaggattt ttctcatata
    22441 atactcttcg aaaatctctt caaactacgt cagcttccat ctgcaacctc aaaacagtat
    22501 tttgagctga cttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgtg
    22561 gctagcttcc tagtttgcgc tttgattttc attgagtata agggaaagta tagtgaattg
    22621 aaataagatg tgaacaactc tatcaggaaa gtcaaattaa tttatagaaa tattttagca
    22681 gccaaggtgt actgttatag attcaattac actataattt agtgtaattg agaaaggaga
    22741 aatgattgtg attgatgttg gctaggttat gttcaatgat tcctaccgtc tcaaatcttg
    22801 tcagtaagga aaaataaatt cttcaaaagt agagattaca aggcttgttt aagaaagaat
    22861 tcaaagacct tgacaaataa aaataaaatg gttattataa aaaatggtct gaaatagatg
    22921 atgatacttt tcgaaaatct cttcaaacta cgtcagctca gctttgcctt gctgtgtttt
    22981 gagcaagcta cggttagctt ccgagtttga ttttcattta ctagaaatga aactgatgag
    23041 agatatcagt agacatttga gtcaggatat tatggaaaat gataaaaaga gctcgtgaga
    23101 ttggcatatc agactactaa agtattgagt ttgttaggat tttagcgact agttagctgg
    23161 gaaaggaaga tatttgtgac aaataataaa ctgtattcgt tgatagaatt tagaaataaa
    23221 atatatgaag aattagaact ttccagaagt gatttagcga ttttactatg tgccatgctt
    23281 atcgcctcta tcggattaaa tatggattcg actcccgtga ttattggagc catgttaatc
    23341 tctcctttga tgacacctat tctgggagtg gggctctctc tagctatatt tgattttaaa
    23401 ttgttaagaa aatcttttaa aatattagct attcaaattc ttgccagtct aatagcttca
    23461 acactttatt tttatctttc tcccatttcg tatgctagtt cggagattgt tgctagaacc
    23521 tctccgacta tttgggatgt tctcattgct tttgtaggag ggatagcagg tatcattggt
    23581 gctaggaaaa aagagaccaa taatattgtt cctggtgttg ctattgcaac cgccttgatg
    23641 cctcctcttt gtacagtagg ttatgctatt gcttctgcta atctaaaatt tatcataggc
    23701 tcctcttacc tattcctcat caattgtagc tttattgtca ttgcgactta tataggtgtt
    23761 aggttgatga tggttaagaa acattatttt aaagataatg aagaagactc taaaatgcgt
    23821 aggattttgc ttctagttgc tgttttgctg atgattccga gtttcatctc tgcaacgact
    23881 ttagtgagag aaacgttgaa aaaagagtcc cttaagaaat ttatatcaga gcagtttcag
    23941 gggtataata ttttgaaaaa aacctattct aaaaagactc ataccctaaa gctaaccatt
    24001 tcaggaaatt atttgacaga agaagaactc gatatgattt caagcaagag aggtgactat
    24061 ggtttaagtg atgtttctgt tcaagtttca caattgtctg attcagaaca acttagtaag
    24121 gaagagctgg tggagtattt cttccagtat atcaaggata aggaagcaaa agaaaaggag
    24181 aaagccaata aatttgatac agagtctgag gagcaataat ttcttgagaa tagctggttt
    24241 ctctcgtgag tcttctatgt atatcaaagg aagactgagg ttttaagtat aaaacttttc
    24301 ttctattata gtagctaagt cttgacaggg ttcaaaaaat ggtttacact ttataaagtt
    24361 tattactttg aaaaggtgtg atactgtggc tacggcaaca aaaaagaaaa aatcaacagt
    24421 taaaaaaaat ctagtcatcg tggagtcgcc tgctaaggcc aagacgattg aaaaatatct
    24481 aggcagaaac tacaaggttt tagccagtgt cgggcatatc cgtgatttga agaaatccag
    24541 tatgtccgtc gatattgaaa ataattatga accgcaatat attaatatcc gaggaaaagg
    24601 ccctcttatc aatgacttga aaaaagaagc taaaaaagct aataaagttt ttctcgcgag
    24661 tgacccggac cgtgaaggag aagcgatttc ttggcatttg gcccatattc tcaacttgga
    24721 tgaaaatgat gccaaccgtg tggtcttcaa tgaaatcacc aaggatgcag tcaaaaatgc
    24781 ttttaaagaa cctcgtaaga tcgatatgga cttggtcgat gcccaacaag ctcgtcggat
    24841 cttggatcgc ttggtagggt attcgatttc gcctattttg tggaagaagg tcaagaaggg
    24901 cttgtcagca ggtcgcgttc agtccattgc ccttaaactc atcattgacc gtgaaaatga
    24961 aatcaatgcc ttccagccag aagaatactg gacagttgat gctgtcttta aaaagggaac
    25021 caaacaattt catgcttcct tctatggagt agatggtaaa aagatgaaac tgaccagcaa
    25081 taacgaagtc aaggaagtct tgtctcgtct gacgagtaaa gacttttcag tagatcaggt
    25141 ggataagaaa gagcgcaagc gcaatgctcc tttaccctat accacttcat ctatgcagat
    25201 ggatgctgcc aataaaatca atttccgtac tcgaaaaacc atgatggttg cccaacagct
    25261 ctatgaagga attaatatcg gttctggtgt tcaaggtttg attacctata tgcgtaccga
    25321 ttcgactcgt atcagtcctg tagcgcaaaa tgaggcggca agcttcatta cggatcgttt
    25381 tggtagcaag tattctaagc acggtagcaa ggtcaaaaac gcatcaggtg ctcaggatgc
    25441 ccatgaggct attcgtccgt caagtgtctt taatacacca gaaagcatcg ctaagtatct
    25501 ggacaaggat cagcttaagc tatataccct tatctggaat cgttttgtgg ctagccagat
    25561 gacagcggcc gtttttgata ccatggctgt taaattgtct caaaaagggg ttcaatttgc
    25621 tgccaatggt agtcaggtta agtttgatgg ttatcttgcc atttataatg attctgacaa
    25681 gaataagatg ttaccggaca tggttgttgg agatgtggtc aaacaggtca atagcaaacc
    25741 agagcaacat ttcacccaac cgcctgcccg ttattctgaa gcaacactga ttaaaacctt
    25801 agaggaaaat ggggttggac gtccatcaac ctacgcgcca accattgaaa ccattcagaa
    25861 acgttattat gttcgcctgg cagccaaacg ttttgaaccg acagagttgg gagaaattgt
    25921 caataagctc atcgttgaat atttcccaga tatcgtaaac gtgaccttca cagctgaaat
    25981 ggaaggtaaa ctggatgatg tcgaagttgg aaaagagcag tggcgacggg tcattgatgc
    26041 cttttacaaa ccattctcta aagaagttgc caaggctgaa gaagaaatgg aaaaaatcca
    26101 gattaaggat gaaccagctg gatttgactg tgaagtgtgt ggcagtccaa tggtcattaa
    26161 acttggtcgt tttggtaaat tctacgcttg tagcaatttc ccagattgcc gtcataccca
    26221 agcaatcgtg aaagagattg gtgttgagtg tccaagctgt catcagggac aaattattga
    26281 gcgaaaaacc aagcgtaatc gcctattcta tggttgcaat cgctatccag aatgtgaatt
    26341 tacctcttgg gacaagcctg ttggtcgtga ctgtccaaaa tgtggcaact tcctcatgga
    26401 gaaaaaagtc cgtggtggtg gcaagcaggt tgtttgtagc aaaggcgact acgaggaaga
    26461 aaagattaaa taagaggagg ggcccgaaag taaatttcag gctccttttg gttagaactt
    26521 gacaaaaaac atcattttat gcgaaactag aagaagatta ttttatactc aatgagaatc
    26581 aaagagcaaa ttagaaagct agtcgcaggt tgctcaaagc actactttga ggttgaagat
    26641 ggaagctgac ctggtttgaa gagattttcg aagagtatta actaggagaa gttatgcgta
    26701 ttatctatct aattattggt tttttatcgc tgaccttggc tattgttggg gttgttttac
    26761 ccttgttgcc tacaacacct ttccttttgt tgtctattgc ttgtttctcc agaagttcca
    26821 agcgattcga agattggctt tatcatacca agctctatca agcatatgta gctgattttc
    26881 gtgagaccaa gtctattgcg cgtgaacgaa agaaaaaaat catcgtctct atctacgtct
    26941 tgatgggaat ttctatttat tttgcacctc ttttaccagt caaaatcggt ctgggtgctt
    27001 tgaccatctt tattacttat tatctcttca aggtcattcc agacaaagaa tagttaaaac
    27061 agtagttatt tgccttgata aaattgaaag catattcata acaatatgat ataataaaat
    27121 tgaagtaata ttcaaggaga atcaaatgat ttacgaattt tgtgctgaaa atgtgacttt
    27181 acttgaaaaa gcgatgcagg ctggagctcg tcggattgaa ctctgtgata atctagcagt
    27241 tggtgggaca acacccagct atggagtgac taaggcagcg gttgaactgg cagctaacta
    27301 cgatacaacc atcatgacca tgattcggcc acgtggtggt gactttgtct ataatgacct
    27361 agaaattgct atcatgctag aagacattcg tttgactgct caggctggaa gtcaaggggt
    27421 tgtatttgga gctttaactg ctgataaaaa gttggataag cctaatctgg aaaagttaat
    27481 tgctgcatca aaaggaatgg aaattgtctt tcacatggcc tttgatgaac taagtgatga
    27541 agatcaagcg gaagctattg actggctcag tcaagccggt gtcactcgta tcctaactcg
    27601 tgctggtgtg tctggcgact ccttagaaaa acgttttgtt cactatcaca gaattttgga
    27661 gtacgctaaa ggtaaaattg aaattctacc aggtgggggg attgaccttg aaaaccgtca
    27721 aacctttatc gaccaggtgg gggtaacaca attgcatggt actaaggttg ttttttaaaa
    27781 aatagaaagg aactgctagc tttgggtagc agttttcact tatgtttgaa atttttaaat
    27841 cctatcaatt taatcaagaa aaggctcatg attatggttt tatagaaaat agcgaagtct
    27901 ggacatatag ttgccagatt ttgcaaggtg actttgtcat gactgtgtcc atcactgctg
    27961 ataatgtgaa ctttcaagtc tttgaccaag agactggtga cctctatcct cacgtttata
    28021 tggaaagcat gaggggaagt tttgtcggaa atgtccgtga ggcttgtctg gagattcttt
    28081 accagattcg gaaggcttgt tttgatgtgc aagattttat ctgtcatcag actaagcgta
    28141 tcatgactca agttcaggaa aagtatggaa accagttgga gtatctgtgg gaaaaatcgc
    28201 ctgatacagc tgtattgcgc catgaaggca atcaaaagtg gtatgccgtc ttgatgaaaa
    28261 tctcttggaa taagctggaa aagggcagag aaggacaagt ggaagcagtc aacctcaagc
    28321 atgaccaagt agctaatttg ctttcacaaa aggggattta tccagccttc catatgagca
    28381 agcgctactg gattagtgtg tcccttgatg atactttatc agatgaagaa gtactggaat
    28441 tgatagaaaa aagttggaac ttaacctcta aaaaatgaaa tattttaata attttcatga
    28501 actttcaatt agctaaatat tctttactga agagattttt agaaaatata ggatttacca
    28561 cactagagga atatggtgcc atcttcaaat acctgattga gaatgtcaag acggatcgtc
    28621 agatcatcta ttcgcctcac tgtcatgatg acctcggaat ggcagtggca aatagccttg
    28681 ctgctgtcaa gaatggtgca ggacgtgttg aagggactat caatggtatt agggagcgag
    28741 ctgaaaatgc tgctttggaa gaaattgcag tggctctcaa tattcgccaa gattactacc
    28801 aagtagaaac cagtattgtc ctaaatgaga ccatcaatac gtcagaaatg gtttctcgct
    28861 tctctggtat tccagttcct aaaaacaaag ccgtcgttgg tggcaatacc ttctcccacg
    28921 aatctggtat tcaccaagat ggagtcctta aaaatcctct cacttatgag atcatcacac
    28981 ctgaattggt tggtgttaag attctgcttg gaaaattatc tggtcgccat gcttttgttg
    29041 agaaactgag agaattggcc ctagatttta cagaagagga tatcaaacca ctctttgcta
    29101 agttcaaggc actggtcgat aagaagcaag aaatcacaga tgcagatatt cgagctttgg
    29161 tagctggaac catggttgaa aatccagaag gcttccactt tgatgattta caacttcaaa
    29221 ctcatgcaga taatgacatt gaagcgctcg ttagcctagc caatatggat ggtgagaaag
    29281 tcgaatttaa tgcgacaggg caaggttccg ttgaagcaat ctttaatgct atcgataagt
    29341 tctttaacca atctgttcgt ttggtgtcct acactatcga tgcggtaaca gatggaatcg
    29401 atacccagga tcgggttttg gtcactgttg aaaacagaga tacagaaacc atctttaatg
    29461 cagcagggct tgattttgat gtgttgaagg cttctgctat tgtctatata aacgctaata
    29521 cctttgttca aaaagagaat gcaggtgaga tgggacgcag tgtttcttac cacgatatgc
    29581 ctagtgtgta aaggagaagg ctatggcaaa gaaaatagta gctctagcag gagacggaat
    29641 tggcccagaa atcatggagg ttggtttaga agttctggag gctctagctg aaaaaacagg
    29701 ttttgactat gagattgaca gacgaccgtt cggaggtgca gatattgatg cagcatgacc
    29761 tcccttacct gatgaaaccc ttaaggcaag tagggaagca gatgctatcc tactagtagc
    29821 tatcggtagt cctcagtatg atggagcagt ggttcgccct gaacaaggcc tgatggctct
    29881 ccgtaaggaa ctcaatcttt acgctaatat tcgtcctgta aaaatctttg acagtctcaa
    29941 gcatttgtca ccactcaaac tggaacgaat tgctggtgta gactttgtcg tggtgcgtga
    30001 attgacaggc gggatttact ttggatatca tattcttgaa gagcgcaatg cgcgtgatat
    30061 caacgactat agctatgagg aagtggagcg gattattcgc aaagcctttg aaattgcaag
    30121 aaatcgcaga aaaatcgtta ctagtatcga taagcaaaat gttctagcga cctcaaaact
    30181 ctggcggaaa gtagctgagg aagtcgcaca ggatttccca gatgtaacct tggaacatca
    30241 gctggtagac tcagctgcta tgcttatgat taccaatcct gctaagtttg atgttattgt
    30301 aacggagaat ctttttggag atattttatc tgatgaatca agcgtcttat ctggtacact
    30361 tggggttatg ccatcagcca gtcattctga aaatggacca agtctctatg aacctattca
    30421 cggttcagca cctgatattg caggtcaagg aattgccaat cctatttcca tgattttatc
    30481 agtttccatg atgttgagag atagtttcgg acgttatgag gatgcagagc gtatcaaacg
    30541 tgctgttgag acaagtctgg cggcaggaat tttaacgaga gatataggag gtcaggcttc
    30601 aacaaaggaa atgacggaag ctattattgc aaggttatga agttagacga aaaaattact
    30661 ctagtccttt tgatttggaa tgtcatcatt ttcttgattt atggtattga caaatctaag
    30721 gcaaggagaa gagtttggcg catccctgag aaaatcttac ttattttagc ctttactttt
    30781 ggtggttttg gtgcctggct agcaggaatc atctttcacc acaagactcg aaaatggtac
    30841 tttaaaatag tttggtttct tgggatggtg accacactag tagccttata ttttatttgg
    30901 aggtaatgga tggcagggtc ttcgagggaa tacgctgctt gggctctagc ggactatggt
    30961 tttaaggtcg tgattgcagg atctttcggt gacattcatt acaataatga actcaataat
    31021 ggcatgttgc caatcgttca gcctagagag gttagagaga aactagccca gctaaaacca
    31081 accgaccagg taactgtgga cttggaacaa caaaaaatca tctcaccagt tgaagaattc
    31141 accttcgaga tagatagcga gtggaaacat aaactcctaa atagtttgga tgatatcggt
    31201 attaccttgc agtatgaaga gttgattgct gcttatgaaa aacaacgacc agcctactgg
    31261 caggattaga aaaaatagaa aaggagatat agtaaactga aataagatgt aaacaaatga
    31321 attggagctt aacatccatt tccagcaatt ttttagaaac tacagtggac tattctggat
    31381 tcaacacatt ataaaattat gacaaaacac attcacaaga aggctacgac attttaaaag
    31441 gtgagggcgg atgtatcgtt tgccctacta aagttggtta cattatcatg accagtgaca
    31501 aggcaggact tgagcgtaag ttcgcagcca aagaacgtaa gcgtaacaaa ccaggtgttg
    31561 ttctctgcgg tagcatggat gaactttgcg ctttagcgca actcaaccca gaaattgaag
    31621 cattctacta aaaacattgg gatgaagata ttcttcttgg ttgtatcctt ccttggaaac
    31681 cagaagcctt tgaaaaactc aaagcatacg gggatggccg tgaagaactt attactgatg
    31741 tacgtggtac tagctgtttt gttatcaagt ttggaaaagc aggtgaacaa ttggctgcca
    31801 agctttggga agaaggtaaa atggtctacg cctcatctgc ttcaatgaca aaacgattga
    31861 aactcgctat gagcaaggtg taatggtgtc tatggtcgat aaggacggca aactcatccc
    31921 agaacaagga ggagcacgtt caacttcacc agctccagtt gtgatccgta aagggcttga
    31981 cattgataaa atcatgatgc acctgtcaga tacttttaac tcatgggact accgtcaggt
    32041 tgagtattat taggatagag aagaagtcta gtgttatgag atattaaagc tcctaacact
    32101 gggcttttgt ttagaatttc ttttcttttt ctataggata tggtattcta tgtagaaaat
    32161 atatgttaat aagtaatgcc aatatttaaa catcattagt aaaaggagtt agattgatga
    32221 ataaaagaaa agttagttta gaagattttt ataaatggta tagtctaaat aaagaagagt
    32281 tattaaataa ggcaactgtt ggtgaaaagt ttaatgataa attaaaagaa gagtttctcc
    32341 aggaatggcc tttggatagg attttaacaa tgtcaatcga tgaatatgta ataggaaagg
    32401 gacagcaaaa taagtcttta tgctacgctc ttgagaaggg aaaatacaaa aatctatttc
    32461 ttggaatttc tggtggctca gcttcaaaat ttggtattta ttggaataaa aaaacaaaca
    32521 aatataaaga tcaagctaat aatgagattt cagagttgga tcagcgattt tcaaaattaa
    32581 aatcagattt gtatgaaatt atcaaagaag gtattcgttt taactttgaa aatcctattt
    32641 ttgatatgaa aagatcaaca aatgaattta ttggtcgttc tgctatggtg acaaaattac
    32701 tttgtatcta tactgaggga gatcctttct ttggtgtaaa tattaatagt cagaaagaat
    32761 tttggaacca ctttgtttct cagacaaatc aaggtggacc ttatctgcaa aatcataaaa
    32821 taattgaact ggtgtccaaa acttatcctg agttggagcc atcgaaatta ggaactatgc
    32881 tttttgagta ttctaagctt tttatggaaa ataaggaaga caatagtaca atggattcat
    32941 caaacaattt tcgtcatcaa ttaactcaat ctctattaaa gtctccaaac ctcatcctcc
    33001 gcggtgctcc tggcacggga aaaacttatc ttgctaaaga aattgctaaa gaattaacgg
    33061 atggcaacga agatcaaatc ggatttgtac aatttcaccc atcatatgat tatacggatt
    33121 ttgtagaagg tttaagacca gtatcaaatg gggatggagc tattgagttt aggctacagg
    33181 acggtatttt taaagatttt tgtcagaaag caaaagaaac ccaattgatt ggaggacaag
    33241 ataattttga tgaggcttgg gattcttact tagaatatat aaatgttgct gaagaaaaag
    33301 aatatataac aaaaacatct tacttatctg ttaatagtag acaaaatttg tcagtaaatt
    33361 atgatagtgg tgttccagga tggtcactac ctagcaaata tgtttacgag ttgtataaag
    33421 ataaaaatta taataagcaa gaatactaca aaagtggtgg aaaaactgtc ctagaaacat
    33481 tgagaaagag atttggtttg aaagactatg tttccccaac agaaattgat actgataaga
    33541 attttgtctt catcatcgat gagatcaatc gtggggagat ttctaagatt tttggcgaac
    33601 tctttttctc tatcgacccc ggctatcgtg gtgaaaaagg aagtgtttct acccaatatg
    33661 caaatctaca cgaaactgat gaaaagttct atatccccga aaatgtttac atcatcggaa
    33721 ctatgaatga tattgatcgt tcagtggata cctttgattt tgctatgcgt cgtcgttttc
    33781 gttttgttga agttactgtc gagggtcaag ctggcatgtt ggataaagag ttgaatatcc
    33841 atgcagaaga agcaaaaatt cgtctaagaa acttgaacgc tgctatcgaa aatattcagg
    33901 aattaaacag tcattatcat attggaccaa gttattttct taagttgaag gatgtagatt
    33961 ttgactatga attactctgg tctgattata ttaagcctct cctagaagac tacttgcgag
    34021 gttcttatga tgaggttgaa actttggaaa ctttgaaaaa agcatttgat ctgacaaata
    34081 atgagcaaaa agatcaggca gtagctgatg acaatgaagg cgatgaaaac gatgatgcgg
    34141 attactgata atcaacacaa gattattaaa gaaaaatttg ttgaagaata tcctaaacta
    34201 agcaatcctc ttttagacag aaccttggaa agtctatccc aagatgaacg tattttcatt
    34261 tttccaaatg atttgactca tactcctgat ttggataagg accaaaagat ttttgaaaca
    34321 gtcaatcaga aaatcaagac agggaacgtg attggttttc ttggatatgg tcaggaaaga
    34381 ttaacgattt cctcacgatt ttctgatgag agtaatgacc actttttgca ttatctctta
    34441 aacaaggttc ttcatatcaa tctcactagt ttagatgttg ctttgtctcg tgaagagagg
    34501 ctttatcaac ttttggtgta tctctttccc aagtatctac aagctgctat tcgaaaaggt
    34561 ctttataagg aatatcatcg attttctcat aacgacagtc atgttaaggg agtgattgat
    34621 gtaagaaacc atctcaagaa aaatcttcct ttcacgggaa atattgccta cgcaacgaga
    34681 gagttcacct atgataatcc cctcatgcag ttggtccgtc acactattga atacattaag
    34741 aatcagaaaa gcattggtca aggggtacta gataatctct caactagtcg tgaaaacgta
    34801 tctgaaatcg tgcgtgtaac gccctcttat aaactagctg atcgtgctaa gattattcgg
    34861 ggaaatcaat ctaaacctat acgtcatgca tactttcacg agtacagaaa cttacaagaa
    34921 ctttgtctga tgatcctaaa ccaagaaaag cacggtttag ggtatcaaga tcaaaaaatc
    34981 tatggtattc tctttgatgt tgcctggctt tgggaagagt atgtttacac cttgttgcca
    35041 aaaggttttg tacatcccag aaataaggat aagacggatg gaatttcagt attttctgtt
    35101 gggaaacgaa aagtatatcc agatttttat gacagagaac gaaagattgt tctagatgca
    35161 aaatataaaa aactggaatt gactgaaaaa ggaatcaacc gtgaggactt attccagctg
    35221 atttcctatt cttatatttt aaaagctgag aaggctggac tgatttttcc tagtatggag
    35281 cagtcagtaa atagtgaaat aggaaaagta gctggctatg gagctcaatt gaagaagtgg
    35341 tctattcgaa tccctcagaa tgcctcattc tatagtacat tttgtaaaat gatggaaaat
    35401 tcagaagaaa attttaaagc gattattgat gaagaagtgg ggagaaaata atttcccctc
    35461 tttcttttat ttttaacaca agtaaaaccc gaacgatata ttttaattca taaaataatt
    35521 tgacaaaaac tgtactttga tttataataa attaaggaaa cgtcggaaaa ggcgtagtga
    35581 tgcaagagca taggtaggtc attacaaaag aaacgagaca tcgatatgtt aaatgaattt
    35641 ccaatttttg attacgaaga tattcaattg attccaaata aatgtgtcat taaaagccgt
    35701 gcagaagcgg atacaagtgt cactttagga aatcacacct ttaaactacc tgttgtgcca
    35761 gcgaatatgc agacgatttt ggatgaaaat gtagcagagc aactggctaa aggtggttac
    35821 ttctacatta tgcaccgttt tgatgaggca ggacgcattc cttttattaa acgaatgcac
    35881 aatcaagggc tcattgcttc tatctctgtc ggtgttaagg attatgagta tgattttgtt
    35941 agccagctca aggctgatac tcctgagtat atcactattg atattgctca tggtcatgcg
    36001 gatagcgtga tttctatgat tcaacacatc aagaaagaat tgccagatac ctttgtcatt
    36061 gctggaaatg tgggaacacc agaagctgtg cgtgaattgg aaaatgctgg tgcggatgct
    36121 actaaggtcg gaatcggtcc tggtaaggtt tgtatcacca aggttaagac tggttttggt
    36181 acaggtggtt ggcagttggc tgctctacgc tggtgtgcca aggctgcgcg taaaccgatt
    36241 atcgctgatg gaggaattcg tactcacggt gatattgcta agtctatccg cttcggtgct
    36301 agcatgatca tgattggttc cctctttgca ggacacattg aaagtccagg gaaaacgatt
    36361 gaagtcgatg gtgaacagtt caaagaatat tatggttcag cctcacaata tcaaaaaggt
    36421 gcttacaaaa atgtggaagg caaacgtatc ttgcttcctg ctaaaggtca tctgcaagac
    36481 actttaactg agatggaaca agaccttcaa agtgctattt cttatgcagg tggacgtcag
    36541 gttgctgacc ttaaacacgt tgattatgtg atcgtgaaaa actccatctg gaatggggat
    36601 gcttcccact aagacggggt ctttgaccaa aaaacttgtt attagagcaa atttctgtta
    36661 taataaaaca agtttccacc cttagtgtaa tggatatcac gtaagattcc ggttcttgag
    36721 atgggggttc gattccctca gggtggatgt aaacatccta aaaagccttt aatagggctt
    36781 ttttctttat cctgacccaa atttgccgct aaaactgaga atctctgtct aaagaattcc
    36841 caaatgcttt ccagtcccgt tttagagtga ctcagggggc tttttttgat ataataaaaa
    36901 ggactattat cagttagaaa gaggttggta tgaaagaatt acaaactgta ctaaagaatc
    36961 attttgcaat cgaatttgca gacaaaaagt tactggaaac tgcctttact catacgagtt
    37021 atgccaatga gcaccgcctc ttaaaaattt cacacaatga acgcttggaa tttttaggag
    37081 acgctgttct acagttattg atttcagaat atctatataa aaaatatcct aaaaagcctg
    37141 aaggtgacct atcaaaactc cgtgctatga ttgtccgtga ggagagttta gctggttttg
    37201 cgcgtgattg ccagtttgac cagtttatca agttgggtaa aggggaagaa aaatcaggtg
    37261 gtcgcaatcg tgacaccatt cttggtgatg cctttgaagc ctttcttggt gcccttcttt
    37321 tggataagga tgtggccaag gtcaaggaat ttatctatca agtcatgatt cctaaggttg
    37381 aagcaggcga gtttgagatg attacagact ataaaaccca tctccaagag ttgcttcagg
    37441 tcaatggtga tgtggctatt cgttatcagg tgatttctga aacagggcct gctcacgata
    37501 aggtttttga tgtagaagtt cttgttgaag gtaagagcat cggtcaaggc caaggtcgtt
    37561 ctaagaaatt agcagagcag gaagctgcca aaaatgccgt tgagaagggg ctggattcat
    37621 gtatttaaag gaaatcgaaa ttcaggggtt caagtctttt gctgataaga ccaaggtcgt
    37681 ttttgaccaa ggtgtgacgg cagttgttgg acccaatgga tctggaaagt ccaatattac
    37741 agaaagtctg cgttgggctt tgggggagtc tagtgtcaag agtctccgtg ggggcaagat
    37801 gccggatgtc atctttgctg gaaccgaaag tcgcaaaccg ctcaattatg cttctgtagt
    37861 tgtgactctg gataatcatg acggatttat caaggatgca ggtcaagaaa tcagggtgga
    37921 acgccatgtc tatcgtagtg gagatagcga atacaagatt gacggcaaga aagtccgtct
    37981 gcgtgatatt catgacctct tcttggatac tggattggga cgagattcct tctctattat
    38041 ttcccaaggg aaggttgagg agatttttaa ttccaagcct gaggaacgac gagctatttt
    38101 tgaagaagct gctggagttt taaaatacaa gactcgcaga aaagaaaccg agagtaaact
    38161 gcagcaaact caggataatc tggaccgctt agaggacatt atctacgagt tggataatca
    38221 aatcaagcct cttgagaagc aagctgagaa tgcccgtaag tttttagact tggaaggaca
    38281 acgtaaggct atttatttag atgttctggt tgctcaaatc aaggaaaata aggctgaact
    38341 agagtcgaca gaagaagagt tgactcaggt tcaagaactc ttgatgagtt attaccaaaa
    38401 gcgtgaaaaa ttagaagaag aaaatcaaac tcttaaaaag caacgccaag atttacaggc
    38461 tgaaatggcc aaagaccaag gcagtttgat ggacttgact agtctgatta gtgatttaga
    38521 aagaaaatta gccctatcga aactggagtc cgagcaagtg gccttgaatc aacaggaggc
    38581 acaagcccgt ttggctgctt tggaggataa gagaaattca ctcagcaaag aaaagtctga
    38641 taaagaaagc tctttagctc tgttagaggg aaatctagtc caaaataatc aaaaactcaa
    38701 tcgtttagaa gctgaattgc tggctttctc agacgatcct gatcagatga ttgagctctt
    38761 acgtgaacgc tttgtagctc ttttacaaga agaagcggat gtctcaaacc agttgacccg
    38821 tattgagaat gagttggaaa atagtcgtca gctttctcaa aaacaagcag atcaactaga
    38881 aaagctgaaa gagcaattag ctatagctaa agagaaggct agtcagcaaa aggaagagct
    38941 tgaaactgcc aaggagcagg ttcagaaatt attggctgac taccaagcta ttgccaagga
    39001 gcaagaggag cagaaaactt cctatcaagc tcaacaaagc caactctttg accgtctgga
    39061 taatctcaaa aacaagcagg ccagagctca aagtttggaa aatatcctga gaaatcatag
    39121 taacttttat gcaggtgtta agagtgttct ccaagaaaaa gatcgcctag gtgggattat
    39181 tggtgcagtc agtgagcatc tgacctttga tgtttattat caaactgccc tagagattgc
    39241 cttaggggca agtagccagc atatcatcgt agaagatgaa gagtcggcaa ccaaggctat
    39301 tgacttcctt aaacggaaca gagccggtcg tgcaaccttt cttcctttga ccactatcaa
    39361 ggcgcgtacg atttctagtc agaaccaaga tgctatcgct gtaagcccag gtttccttgg
    39421 gatggcagat gagttggtga cttttgatac tagactggaa gccattttca agaacttgct
    39481 agctacgacg gctatctttg ataccgtaga acatgcgcgt gcagcagctc gccaagttcg
    39541 ttatcaggtt cgtatggtga cattggatgg gacagagttg cgcacaggtg gttcttatgc
    39601 aggtggtgcc aatcgccaga ataacagcat ttttatcaag ccagaactgg agcaattaca
    39661 aaaagaaatt gctgaagaag aagcaagctt gggttcagaa gaggcgactt tgaagacctt
    39721 gcaagatgag atggccagat tgaccgagtc attagaagct attaaatctc aaggagagca
    39781 ggcacgtatt caggagcaag gcttgttcct cgcttatcag caaactagtc agcaagttga
    39841 agaactggaa actctttgga aactccaaga agaggaaata gatcgtcttt ccgagggaga
    39901 ttggcaagcg gataaggaaa actgccaaga gcgtcttgct gcaatctcca gtgacaagca
    39961 aaatctggaa gctgagattg aagagattaa gtctaacaaa aatgccatcc aagaacgcta
    40021 tcaaaacttg caggaagagc tagcgcaagc tcgtttgctt aagacagaac tgcaagggca
    40081 aaaacgttat gaagttgctg atattgaacg cttaggcaag gaattggaca atcttgattt
    40141 tgaacaagag gaaatccagc gccttcttca agaaaagatt gataatcttg agaaggttga
    40201 tacagaattg ctcagtcaac aggcggaaga atccaaaact caaaaaacaa atctccaaca
    40261 aggtttgatt cgcaagcagt ttgagttgga tgatattgag ggtcaactgg atgatattgc
    40321 tagtcatttg gatcaggctc gccagcagaa tgaagagtgg attcgcaagc aaactcgtgc
    40381 tgaagccaag aaagaaaagg ttagcgagcg cttgcgccat ctacaaagtc aattaacaga
    40441 ccagtaccag atcagctaca cagaagcttt agaaaaggcg catgagttgg aaaatctcaa
    40501 tctggcagag caagaagtta aggatttaga gaaggctata cgctcactgg gtcctgtcaa
    40561 tttagacgct attgaacagt acgaagaagt tcacaaccgt ctggatttcc taaatagcca
    40621 acgtgatgat attttgtctg cgaaaaacct gctccttgag accattacag agatgaatga
    40681 tgaggtcaag gaacgcttta aatcaacctt tgaggtcatt cgtgagtcct ttaaagtgac
    40741 cttcaagcag atgtttggtg gaggtcaggc tgacctcatt ttgactgaag gagacttgct
    40801 gacagctggg gttgaaattt ctgtccaacc accaggcaag aaaatccagt ctcttaatct
    40861 catgagtggt ggtgaaaaag ccctatcggc tcttgccttg ctcttctcca ttattcgtgt
    40921 caagactatt ccatttgtta tcttggatga ggtggaagct gcgctggatg aagccaatgt
    40981 taaacgtttt ggggattacc tcaaccgctt tgacaaggac agccagttca tcgtcgtaac
    41041 ccaccgtaag ggaaccatgg cagcggctga ttccatctat ggagtgacca tgcaagaatc
    41101 gggtgtttca aagattgttt cagttaagtt aaaagattta gaaagtattg aaggatgaca
    41161 attaaactag tagcaacgga tatggacgga accttcctag atgggaatgg acgctttgat
    41221 atggatcgtc tcaagtctct cttggtttcc tacaaggaaa aagggattta ctttgcggta
    41281 gcttcgggtc ggggatttct gtctctagaa aaattatttg ctggtgttcg tgatgacatt
    41341 attttcatcg cggaaaatgg cagtttggta gagtatcaag gtcaggactt gtatgaagcg
    41401 actatgtctc gtgactttta tctggcaact tttgaaaagc tgaaaacttc accttatgta
    41461 gatatcaata aactgctctt gacgggtaag aagggttcat atgttctaga tacggttgat
    41521 gagacctatt tgaaagtgag tcagcactat aatgaaaata tccaaaaagt agcgagtttg
    41581 gaagatatca cagatgacat tttcaaattt acaaccaact tcacagaaga aacgctggaa
    41641 gatggggagg cttgggtaaa cgaaaacgtt cctggtgtta aggccatgac aactggcttt
    41701 gaatccattg atattgttct ggactatgtc gataagggag tggccattgt tgaattagtt
    41761 aaaaaacttg gtatcacaat ggatcaggtc atggcttttg gagacaatct taatgactta
    41821 catatgatgc aggttgtggg acatcctgta gctcctgaaa atgcacgacc tgaaatttta
    41881 gaattagcaa agactgtgat tggtcaccat aaggaacggt cggttatagc ttatatggag
    41941 ggcttataat ggcagatata aaattgattg cattggactt ggacgggacc ttgctgacta
    42001 ctgataaaag gctgacggat cgtaccaagg aaaccttgca agctgcgcgt gatcgtggta
    42061 tcaaggtcgt attgacaact ggtcgtccct taaaagccat ggatttcttt ctccatgagt
    42121 tagggactga cggtcaggaa gatgagtata ccattacttt taatggtgga ttagttcaga
    42181 aaaatacagg agaaatcctt gataaaacag tcttttcata tgatgatgtg gcacgtttgt
    42241 atgaagaaac agagaaatta tcactgcctc ttgatgccat ctcagaagga acagtttatc
    42301 aaatccaatc ggaccaagaa agtctttatg ccaaattcaa tccagctttg acctttgttc
    42361 cagtggactt tgaagactta tctagtcaaa tgacctacaa caaatgcgtg actgcctttg
    42421 ctcaagaacc cttggatgca gccattcaga agatttctcc agaattgttt gaccaatatg
    42481 aaatctttaa atcacgtgaa atgttgctag aatggtcacc aaagaatgtt cataaagcaa
    42541 caggtttggc aaaactaatc agccatcttg gaatcgacca aagtcaagtg atggcttgtg
    42601 gtgacgaggc caatgacctc tctatgattg aatgggcagg tcttggtgtt gctatgcaaa
    42661 acgctgttcc tgaagtaaag gcagccgcaa atgtagtgac gccgatgacc aacgatgagg
    42721 aagctgtcgc ctgggctatc gaagaatatg tgctaaagga gaactaagat atgggattgt
    42781 ttgaccgtct attcggaaaa aaagaagaac ctaaaatcga agaagttgta aaagaagctc
    42841 tggaaaatct tgatttgtct gaagatgttg atcctacctt cacagaagtt gaggaagttt
    42901 ctcaggaaga agcagaggtt gaaattgttg aacaagctgt gttccaagaa gaggaaatcc
    42961 aagacacagt tgaagaaagt caggattcag agccagctgt aggggtttct caagaagaaa
    43021 tagaagaatt accaaactca gaagaattac cagaggaaga gaagcttgag cacgaaggaa
    43081 ctgtagaaga aaataattct gaagtgcttg aaccagaagc tcctcaaaca gaagaaactg
    43141 ttcaggaaaa atatgaccgc agtcttaaga aaactcgcac aggtttcggt gcccgcttga
    43201 atgccttctt tgctaacttc cgctctgttg acgaagaatt tttcgaggaa ctggaagaac
    43261 tgctgattat gagtgatgtt ggtgtccaag tcgcttctaa cttaacggag gaactacgtt
    43321 acgaagccaa gcttgaaaat gccaagaaac ctgatgtact tcgtcgtgtc atcattgaga
    43381 aattggttga gctttatgaa aaggatggta gctacgatga aagcatccac ttccaagata
    43441 acttgacagt tatgctcttt gttggtgtga atggtgttgg gaaaacaact tctatcggaa
    43501 aactagccca ccgctacaaa caagctggta agaaggtcat gctggttgca gcagatacct
    43561 tccgtgcggg tgcagtagct cagctagctg aatggggccg acgagtagat gttccagtag
    43621 taactggacc tgaaaaagct gatccagcca gcgtggtctt tgatggtatg gaacgtgccg
    43681 tgactgaagg tatcgatatt ctcatgattg atactgctgg tcgtctgcaa aataaggata
    43741 accttatggc tgagttggaa aagattggtc gtattatcaa acgtgttgtg ccagaagcac
    43801 cacatgaaac cttcttggca cttgatgcat caacaggtca aaatgcccta gtacaggcca
    43861 aagaattttc gaaaatcaca cctttaacgg gaattgtttt gactaagatt gatggaactg
    43921 ctcgaggagg tgtggttcta gccattcgtg aagaactcaa tattcctgta aaattgattg
    43981 gttttggtga aaaaatcgat gatattggag agtttaactc agaaaacttt atgaaaggtc
    44041 tcttggaagg tttaatctaa tcagaagcaa aaatcctgca aggcataaac ttgcaggaaa
    44101 tttttttatt ctaagcgacc atcttgacga taggtgatat ctggttgcca agtccatttg
    44161 gcaccgaatt tttcaagtag gtcaaagctg gcttgaggtc ccatgcttcc agctttatag
    44221 tcatgaagtg gggcaccatt ttcagcccag agcttttcaa tacggtcaat caacttccat
    44281 gacgcacaaa cttcatccca gtggctaaag ttagttgagt tgttatttag gacatcataa
    44341 atcaattttt cgtatggttc tggagaagca ccagttgcag tcgcatctgt acggtaatca
    44401 agtgagttag gagccaagtt aaattcttct cctacttgct tcccatttag gctaagagag
    44461 aagccttctg ttggttgaat atagatggtc aaaatatttg gagcaagtgg ttctccaaag
    44521 atagaatcca tttgtttaaa gacgatgttg acatgagttc ctttttcagt cagtcgttta
    44581 cctgtacgga aaaagaaagg aacaccacgg aatcgatcgc tgtctacaaa gaaggcacca
    44641 gatgtaaagg tttcagttgt tgattctgga ttcacatttg gctcgctacg ataagagatg
    44701 tatttcatgc catcaatctt accagagcgg tattgcccac ggataaagtg ttctttgagt
    44761 tcttcatcag ttggatgata gaggttttta aagaccttaa tcttttcagc acgaatctcg
    44821 tcttttgtga agcttgctgg tttgtccatg gcgaggagcg aaagaagttg tagagtgtgg
    44881 ttttggacca tgtcacggag ggcaccggat tggtcatagt agccaccacg ttcttctaca
    44941 cccaagcgct ccgcaaaggt aatttgaaca ttgtcgataa aatccttgtt ccaaacgttt
    45001 tcaaaaatca agtttgcaaa gcgaactgca aagatgcttt ggatcatttc cttaccaaga
    45061 taatggtcga tacggaaaat ttgttcttcg tcaaatgttg ctaggagttc gtcattcaac
    45121 ttgcttgcag ttgcgtaatc tgtaccaaat ggtttttcaa cgatcaagcg ctcaaaacct
    45181 ttgccatcga caatgttttc agatttgagg tgtttggcaa tggttccaaa gaactgaggt
    45241 gccatagaca agaagaagag cttattgtgt tcagcttggt atttttcatt aagctcagct
    45301 tgtaattgac gcaaagcaat ataatgttcc gaatcattga catcatggct ttgatagtag
    45361 aagtggctag caaattcttg ggcttgctcg gtactatctg ccaaatcaag gatggactcg
    45421 acaactacag attcaaaata ttccttactc caaggtctac gggcagttcc aataacggca
    45481 aagtgcttgg aaagattgcc ggattgatat agtctaaaaa gggaagggta gagcttgcgt
    45541 ttagccaggt ctccactcgc accgaaaatt gtaacaataa ccttagatga catctagcta
    45601 cctaatttct atttttattc ctagtcttgc taggtatgag gaaacctcat ttcataaact
    45661 taattctaac ataattttcc gaaaaatcaa aaattccaat acgaggtaga aaaactgcaa
    45721 ggaaatcctt acagtttgag tttagacgtt taagacctta tctaagaact ctttcagacg
    45781 agggtgttgt gggttatcaa agatttggtc aggtgttccg tcttcaagga actcgccatc
    45841 tgcagtaaag ataacgcggt tggcaacctg acgagcaaat cccatctcat gggttacgat
    45901 aatcatggtc atgccttgct cagccaattc cttcataacg ttaagtacgt ctccaaccat
    45961 ctcagggtca agggcagaag ttggttcatc gaagagcatg atgtctggat tcattgctag
    46021 gccacgagcg atggccacac gttgtttttg accacctgat aggctatctg gattggcatt
    46081 agctttatct gctagtccaa ccttttcaag caactccatt cccaattcct cagcttcttc
    46141 cttagtcatc aacttgtgct caataggagc aaaggtgatg ttgtccaata cagacatatg
    46201 agggaagagg ttgaagtgtt ggaataccat gccgatattt tcacggacgt ggtcaacatt
    46261 ggttgttttt tcagttaaat catagccgtt cacagtgatg tgaccgctag tgacttcttc
    46321 taaaagattg aggctacgga ggaaagttga cttaccagaa cctgaaggac cgatgataca
    46381 aacaacatct ccttcataga acttagtcgt aattcctttt aggacttcat tttttccata
    46441 gtgcttgtgt aaatcattta catcaatttt taattttgcc attaacgaat cctcttttct
    46501 aagcgtttcg ctagtctagt caaaagtgtg ataattacaa gatagaagat agcaaggatt
    46561 gcatacatct tgaaactttg gtagttacga gcaatgataa tcttaccagt ttggaagagt
    46621 tcaaccaaac cgatagcaga tacgatagtt gtatctttaa gagcgataac gaattggttg
    46681 acaaagtttg gcaacatcaa tttagttgct tgtggcaaga taatcttacg catggttttt
    46741 ccataagaga tacccaagct tcggctggct tccatttggc caactggaac ggcctgaata
    46801 ccaccacgaa cgatttcagc gatataagca gccgcattga gtgagagggc aatggttcca
    46861 gctacaaagt cgttaattgg gctttgttgg cctgtgatag actcgatgaa gtttggaatt
    46921 ccccagaaga tgaaggctgc aagaatcatc aatggaatac cacgaataac gtcaacgaaa
    46981 atctcagaga tgacgcgaag agatttgtat gggctaacgc taaacatacc gaagataatt
    47041 ccgatgacaa tggcaatagc aaatgagata agagctagag caagagtgat accaagaccg
    47101 ctaaggagtt gtttgtagtt gttttgaagc aagccccaga gcgttgtttc gtcaacagta
    47161 cttgttgaag cagttgaaga ttcgctagct aggtatttgt caagaatctt ttggaattca
    47221 ccgtttgctt taaggtttgc aagtccgttg ttgaacattt caatcagttc tggatttgct
    47281 ccttttttaa cggcaaaggc tgtttcaccg attggagttc cagagattgg agttttcaat
    47341 ttttgacctt ggctgataga atatttgaga acaggttcat catccataac ggcatcaatg
    47401 gcaccagtgt ttaaactgtc atacattgaa gaaccatcag caaaggtttt gattttgtag
    47461 ccgtatttgc tttgattttc tgttaggaag gtttgagaag cagttccgtt tttaacaccg
    47521 actgtctttc cttttagatc ttcataagaa gcaatattgc ttgattcttt gacaccaaga
    47581 atggtattag cagtgtagta tgattctgag aagtcaaaag ttgccttacg agcatctgtg
    47641 acagacatac cagcgatgat accatcggct tgaccagctt ggacagcact gatagcagca
    47701 tcaaaaccag ggttggtgat ttcaatttca aaaccttggt ctttagcgat tgccttaatc
    47761 aattccatat caataccagt gtattggttg cttgaatttt ggaaaacaaa aggggcaaaa
    47821 gaagaatcgc tggcaatgat atatttagcc ttaacaggaa tagcttttaa tcctgcgaga
    47881 gtagttgtag ttggcactgc tgaagatgat gaagcagtcc atttcttgat aattttatca
    47941 agactaccat cttttttcat ttcagacaag gcttggttaa attcagtaac caggtgctcg
    48001 tatttacttc cttttttcac accgaaagca aaacttccta cagcttcacc atccatttca
    48061 atatggaggt cttgaccttg gttaatggca tattcgataa caggtttgtc atccatcatg
    48121 gcatcgatgg caccagcact caagctgttg ttcattaaat caccagtgtc aaatgtttta
    48181 atagtaaagc cgtatttatc tttgattgtt tcaaggaaac gttgagcggc agttccgttt
    48241 ttaacaccaa cggttttgcc agttaattgg tcgtacttgc taattttgtg tgactttgta
    48301 gtagcaatga caacttttgt atcatagtaa gtatcagaca tggtgaagac tttttcacgt
    48361 tctttagtct ttgtcatccc tgccatgata gcgtcggctt gcccagcttg aaccgcattg
    48421 actgctgcgt caaatccagg ataggacatc tgaatgttcc agcctttaat ctcagcgact
    48481 ttgttaataa tgtcaacatc aattccttta taagtttgat ctgaatcttt aaactcaaaa
    48541 ggtgcatagg cggtatcaga aacaatctta atcgtttctg ctttggcaat acctaatgag
    48601 aaaattggga ataaaattag caaaaatgct agaaattttt tcttcatttt tagtctcctt
    48661 ttccgaatat tctctcatta tatcagaaaa agtaaaaaaa ggcaaatttt tatatttttt
    48721 gtgtcagaaa atataacatc gattttttcc tttccttgaa ttgctattaa aaagtgctat
    48781 aataatagta tatatagaag aaaagaggac ggggatatga agaacaaaag aatatttaaa
    48841 gacttccaag cttcaaaaat gagtttaaac atttacacaa gccccttgtt agcctttgtt
    48901 tttgtcttca taggagagtt tgtggctttt actttgtatg gtattggctt gttagctctc
    48961 atcggacttg ctagaaattt tggagaggct ggtcaaaatc ttgcaagcta cttgcagacc
    49021 ttgcatcaga gcttgacgga taaaacaagt gactttcgtt taattttagg attactggcc
    49081 tttggtttta ttcttaacac tgtgttcaga tggacaagaa aagttgagaa aagacctatt
    49141 cgaaccttgg gattttatag agagaatttc ctcagcaatc ttctgaaagg atttagtcta
    49201 ggcctggcac tttttcttct gaccttgtta ggtttagtgg tcttaggtca atatcgtttg
    49261 gaatccattc acttgaatcc ttattctctt gcctttgtcg tctttactat cccattttgg
    49321 attttacagg ggacagcaga agaagtggtg gcccgtgctt ggctacttcc tcaattggcc
    49381 tcaagaacca atctaaaact agctattctt atatctagcc tgttctttac cctgcttcat
    49441 atgggcaatt ctggtctcac ccctctatct ctagtaaatc tctttttatt cggagttgcc
    49501 atggctcttt accttctcaa aactgataca gtttggggtg ttgcaggtat tcatggtgct
    49561 tggaattttg ctcagggtaa tctctttggg attttagtta gtggtcaacc gtcaggaacg
    49621 tctctgatga cctttttacc acaaggcaat caagattggc tatcaggtgg ttcttttggc
    49681 atagaaggtt ccattatgac aagtctggta ttactactgc tgattgtcta tcttgctaat
    49741 aaattaaaga aagaaaatga aaggatgtga cttcggtccg tccttttctt cgtgaaaata
    49801 ctataagtat gctaaaatag gaatagcaca tggagagagg attcttatga tcaatcacat
    49861 tacagataat caatttaaac tagtatcaaa atatcaacca tcaggagatc aaccccaagc
    49921 tatcgagcag ttggtggata acattgaggg gggagaaaaa gctcagattc tgatgggggc
    49981 gactgga
//
LOCUS       DBDESG010000006        49106 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.006, whole genome shotgun sequence.
ACCESSION   DBDESG010000006 DBDESG010000000
VERSION     DBDESG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 49106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.006"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            62..529
                     /locus_tag="ACKB38_000331"
     CDS             62..529
                     /locus_tag="ACKB38_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979174.1"
                     /translation="MIDKVVRNLLLTFFFCKMTKIIIFLTTILVKKKKICYNEFKLRN
                     RKQKGVIMWVLGFILFMIFFYSNNSKKIKKLENKIKRLERKEKGNAEMSRLLQEMIGK
                     EPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            539..745
                     /locus_tag="ACKB38_000332"
     CDS             539..745
                     /locus_tag="ACKB38_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979175.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            745..942
                     /locus_tag="ACKB38_000333"
     CDS             745..942
                     /locus_tag="ACKB38_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979176.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            945..1678
                     /locus_tag="ACKB38_000334"
                     /pseudo
     CDS             945..1678
                     /locus_tag="ACKB38_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
     gene            1690..2331
                     /locus_tag="ACKB38_000335"
     CDS             1690..2331
                     /locus_tag="ACKB38_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGL7979177.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2349..2579
                     /locus_tag="ACKB38_000336"
     CDS             2349..2579
                     /locus_tag="ACKB38_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979178.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2621..2812
                     /locus_tag="ACKB38_000337"
     CDS             2621..2812
                     /locus_tag="ACKB38_000337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979179.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTWFEEIFEEY"
     gene            2931..4694
                     /gene="aspS"
                     /locus_tag="ACKB38_000338"
     CDS             2931..4694
                     /gene="aspS"
                     /locus_tag="ACKB38_000338"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGL7979180.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4672..5613
                     /locus_tag="ACKB38_000339"
     CDS             4672..5613
                     /locus_tag="ACKB38_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGL7979181.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5617..5763)
                     /locus_tag="ACKB38_000340"
     CDS             complement(5617..5763)
                     /locus_tag="ACKB38_000340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979182.1"
                     /translation="MKIKVQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     FEEY"
     gene            complement(5905..6891)
                     /locus_tag="ACKB38_000341"
     CDS             complement(5905..6891)
                     /locus_tag="ACKB38_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7979183.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(6901..7701)
                     /locus_tag="ACKB38_000342"
     CDS             complement(6901..7701)
                     /locus_tag="ACKB38_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGL7979184.1"
                     /translation="MLPYPFSYFSSIWGVRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASFLLYITKRSRLFSFNTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(7954..8796)
                     /locus_tag="ACKB38_000343"
     CDS             complement(7954..8796)
                     /locus_tag="ACKB38_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGL7979185.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8798..10090)
                     /locus_tag="ACKB38_000344"
     CDS             complement(8798..10090)
                     /locus_tag="ACKB38_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGL7979186.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10201..11472)
                     /locus_tag="ACKB38_000345"
     CDS             complement(10201..11472)
                     /locus_tag="ACKB38_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGL7979187.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12007..13539
                     /gene="malQ"
                     /locus_tag="ACKB38_000346"
                     /pseudo
     CDS             12007..13539
                     /gene="malQ"
                     /locus_tag="ACKB38_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            13565..15823
                     /gene="glgP"
                     /locus_tag="ACKB38_000347"
     CDS             13565..15823
                     /gene="glgP"
                     /locus_tag="ACKB38_000347"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HGL7979188.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAVVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERFYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(16867..17439)
                     /locus_tag="ACKB38_000348"
     CDS             complement(16867..17439)
                     /locus_tag="ACKB38_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979189.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAVWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            17516..17722
                     /locus_tag="ACKB38_000349"
     CDS             17516..17722
                     /locus_tag="ACKB38_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979190.1"
                     /translation="MLKKYFSKYKWTDLFWILFVILTCLYIGNHDLFTLNHQEFSFRG
                     SVWGLVLALYHLLFIDKFVISNRK"
     gene            complement(18238..19086)
                     /locus_tag="ACKB38_000350"
     CDS             complement(18238..19086)
                     /locus_tag="ACKB38_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGL7979191.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            19238..19516
                     /locus_tag="ACKB38_000351"
     CDS             19238..19516
                     /locus_tag="ACKB38_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGL7979192.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19518..21581
                     /locus_tag="ACKB38_000352"
     CDS             19518..21581
                     /locus_tag="ACKB38_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGL7979193.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21659..22915)
                     /gene="tyrS"
                     /locus_tag="ACKB38_000353"
     CDS             complement(21659..22915)
                     /gene="tyrS"
                     /locus_tag="ACKB38_000353"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGL7979194.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23057..25522
                     /gene="pbp1b"
                     /locus_tag="ACKB38_000354"
     CDS             23057..25522
                     /gene="pbp1b"
                     /locus_tag="ACKB38_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGL7979195.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     VIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPEKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25587..26498
                     /locus_tag="ACKB38_000355"
     CDS             25587..26498
                     /locus_tag="ACKB38_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGL7979196.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26728..27426
                     /gene="dapD"
                     /locus_tag="ACKB38_000356"
     CDS             26728..27426
                     /gene="dapD"
                     /locus_tag="ACKB38_000356"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGL7979197.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGSVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27494..28624
                     /locus_tag="ACKB38_000357"
     CDS             27494..28624
                     /locus_tag="ACKB38_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGL7979198.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28636..29175
                     /locus_tag="ACKB38_000358"
     CDS             28636..29175
                     /locus_tag="ACKB38_000358"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGL7979199.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLQHPFYQEAKVI
                     ATYLSFSHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTFFALLELQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            29159..29836
                     /locus_tag="ACKB38_000359"
     CDS             29159..29836
                     /locus_tag="ACKB38_000359"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGL7979200.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            29850..30035
                     /locus_tag="ACKB38_000360"
     CDS             29850..30035
                     /locus_tag="ACKB38_000360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979201.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAMLTWFEFDFH"
     gene            complement(30137..31078)
                     /locus_tag="ACKB38_000361"
     CDS             complement(30137..31078)
                     /locus_tag="ACKB38_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979202.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDDKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAAEKAKESSSQSNSSGSASNESYNGSSNS
                     NVDYSSSEQTNGYSNNYGGQDYSGSGDSSTNGGSSEQYSSSNSNSGANNVYRYKGTGA
                     DGYQRYYYKDHNNGDVYDDDGNYLGNFGGGIAEPSQR"
     gene            complement(31496..32395)
                     /gene="galU"
                     /locus_tag="ACKB38_000362"
     CDS             complement(31496..32395)
                     /gene="galU"
                     /locus_tag="ACKB38_000362"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGL7979203.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(32417..33433)
                     /locus_tag="ACKB38_000363"
     CDS             complement(32417..33433)
                     /locus_tag="ACKB38_000363"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGL7979204.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            33591..34085
                     /locus_tag="ACKB38_000364"
     CDS             33591..34085
                     /locus_tag="ACKB38_000364"
                     /inference="COORDINATES: protein motif:HMM:NF037495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979205.1"
                     /translation="MIQYMLIIEVNNSGSSCRLREFGEKIKRLRLAKKISRSEFCGDE
                     SELSIRQLIRIENGESRPILTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRT
                     PTYEDETIAQKKESVFDKIFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEI
                     LSFW"
     gene            complement(34200..34631)
                     /locus_tag="ACKB38_000365"
                     /pseudo
     CDS             complement(34200..34631)
                     /locus_tag="ACKB38_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(34693..35343)
                     /gene="phoU"
                     /locus_tag="ACKB38_000366"
     CDS             complement(34693..35343)
                     /gene="phoU"
                     /locus_tag="ACKB38_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGL7979206.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(35358..36110)
                     /gene="pstB"
                     /locus_tag="ACKB38_000367"
     CDS             complement(35358..36110)
                     /gene="pstB"
                     /locus_tag="ACKB38_000367"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGL7979207.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(36112..36927)
                     /gene="pstA"
                     /locus_tag="ACKB38_000368"
     CDS             complement(36112..36927)
                     /gene="pstA"
                     /locus_tag="ACKB38_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGL7979208.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(36920..37783)
                     /gene="pstC"
                     /locus_tag="ACKB38_000369"
     CDS             complement(36920..37783)
                     /gene="pstC"
                     /locus_tag="ACKB38_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGL7979209.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(37901..38776)
                     /locus_tag="ACKB38_000370"
     CDS             complement(37901..38776)
                     /locus_tag="ACKB38_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGL7979210.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(38943..40274)
                     /gene="pnpS"
                     /locus_tag="ACKB38_000371"
     CDS             complement(38943..40274)
                     /gene="pnpS"
                     /locus_tag="ACKB38_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGL7979211.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(40275..40982)
                     /locus_tag="ACKB38_000372"
     CDS             complement(40275..40982)
                     /locus_tag="ACKB38_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7979212.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(41066..41551)
                     /locus_tag="ACKB38_000373"
     CDS             complement(41066..41551)
                     /locus_tag="ACKB38_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGL7979213.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            41537..42094
                     /locus_tag="ACKB38_000374"
                     /pseudo
     CDS             41537..42094
                     /locus_tag="ACKB38_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(42409..44100)
                     /gene="argS"
                     /locus_tag="ACKB38_000375"
     CDS             complement(42409..44100)
                     /gene="argS"
                     /locus_tag="ACKB38_000375"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGL7979214.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            44236..44682
                     /gene="argR"
                     /locus_tag="ACKB38_000376"
     CDS             44236..44682
                     /gene="argR"
                     /locus_tag="ACKB38_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGL7979215.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            44733..47267
                     /gene="mutS"
                     /locus_tag="ACKB38_000377"
     CDS             44733..47267
                     /gene="mutS"
                     /locus_tag="ACKB38_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGL7979216.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            47414..>49106
                     /locus_tag="ACKB38_000378"
     CDS             47414..>49106
                     /locus_tag="ACKB38_000378"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979217.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT        14312 a         9238 c        10590 g        14966 t
ORIGIN      
        1 gtaaccatac ctacggcaag gtgaagctga cgtggtttga agagatttta gaagagtatt
       61 aatgatagat aaagtggtca ggaacctact cctgaccttt ttcttttgca aaatgacaaa
      121 aatcataatt tttttgacaa ctatacttgt caaaaagaaa aagatatgtt acaatgaatt
      181 caagttaaga aataggaagc agaaaggagt tataatgtgg gtactaggat ttatactatt
      241 tatgattttc ttttattcta ataattctaa aaaaatcaag aaactagaga ataaaatcaa
      301 aagacttgag cgaaaagaga aaggaaacgc agaaatgtcg agattattac aagaaatgat
      361 tggaaaggaa ccaattataa cgggagtgta tattgggcca gataactggg aagttgtgga
      421 tgttgatgag gaatgggtaa agctacgacg tgtagataat acgggaaaag aaaaattcaa
      481 gttgcaacgt attgaggata tccaaaccgt tgaatttgac ggagagtagg tgtgtaacat
      541 gcaactaaaa aatcgtctaa aagagcttcg agctcgcgat ggtctcaatc aaaccgacct
      601 agccaaactg gcaggggttt ccagacagac cattagccta ctagaacggg atgagtacac
      661 cccatccatt atcattgccc tgaaaatatc tcaaattttc aacgaaacag tcgaatcggt
      721 atttcgtttg gaggaggacg agtgatgaac aagtataaag tgatttatta tgtagtggtc
      781 atagctttat tagtcagcgt atttctactg attgggatgg acctaagctg gtttaatccc
      841 tatcaaagcg accaatttgt ttgggtctac tttgctctca tcccagtaat tgaatggatt
      901 gaaaagaaat ccaaaaatct agcaagtgaa aaaggagaat gaatatgaaa aagaaacgta
      961 gattgttatt tttgatgtct attgtcttgg ggggttcttg ggcatgtttg tagggatgtt
     1021 taaggcacgt gtcgaatccc acgaaattat tttagatgta aaagccttga tgccatggat
     1081 atcagctatt tgtttactga taggtttcat tagtatgttt ttgactttca atttcttaaa
     1141 gaaaagcaga aaatttcatt ctttgtatca agaggaaatg gatgacgatc tgaatgaaac
     1201 ctattatgtg caaatgtatc ggaatcttga gtttggaacc attgctttta atattacagg
     1261 tgtagcgatt ccattggcta tttttatttc attaagtgag gtgattatat tgcatacaaa
     1321 ccctcaaaca tttttccttt ctttcttact ctttgtggta ttcttagtcg ctcaaaaatc
     1381 tctttttaaa accattgcga ttgttcgtca gtttgatttg gaatttttcg ctacaccaaa
     1441 ggatgtcttg aactatataa attcttatga tgaaggggag cgtcaggcta atttggaaca
     1501 gagttttcga attttattcc aattacacca atatgtctta ccagccttat atatttttct
     1561 tattatcatt tctttcttga caggagagat tcagttacta gctttcttgc ttgtaggagc
     1621 catccatgtt tatatcaatg tgatgcagtt acctatggta aaacgttatt tcaaataaag
     1681 gagttatcta tgaaattact taaaaacctt ggctggattc ttctagccct tctatccttt
     1741 ttatttatct atggctttat tcaggggctc gcgactgcgt cgcttgcttt aggcgcttca
     1801 ccctatgctg ttactctact ctatgtggcc ttggctggag tatatgtgta cggcatttac
     1861 aaatggtatc agaaggctcc tgttcatatt gagaagagcg gctttaatcg atttatttgg
     1921 cttcctgtct tggtttggtt cttatctctg gtcgttcaat tcttcttgcc agatgatcct
     1981 tcagtaaatc agcaaatagc gacagacttg accttgtctc aaccactttt ctcattcttt
     2041 gctgtggtta tttttgctcc tttgacggaa gagattgttt ttagagggat gttagcacgc
     2101 tatctctttc ctaagcagga caatagtaaa cgaaccctga tttttcttct ggtatctagt
     2161 cttctatttg ccttgattca ttttccaggt gatgtgcaac aattttttgt ctattttagc
     2221 cttggtttta gtttgggttt ggcttacatt agcagaaaag gtctggtcta cagtatttct
     2281 cttcacgctt tgaataattt agtcggcttt ttgatgattc tcatgctata atagagtcag
     2341 gaggtcacat gaaacgagta attttattag cagtgattca ggcagtcgtt ctatttttca
     2401 tcattggagc gctagcttat gccttcaaag gcgatttctt ctataactat ctagcagttg
     2461 tctttgctcc tattgcaggt gtactgcgtt ttgggacggc ttacataacg gaaattgtct
     2521 tgcctcgaaa ggcagccgaa atcgctgaaa agcgtaaagc aggcaaaaat tcaaaataaa
     2581 agagtccggt gaagttcatc ggatttttgt attatactca atgaaaatca aagagcaaac
     2641 taggaagcta gccgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacga
     2701 agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
     2761 atacggtaag gcgacgctga cgtggtttga agagattttc gaagagtatt agcgctaatt
     2821 tttagggctt tcacctgact tttaaagact ggcaaacttt tctgatgatt ttcatgaaga
     2881 gcctgcgctt ttatggtaaa atagtaacag aataaaagag gagagaaata atgaaacgta
     2941 gtatgtatgc tggtcgtgtt cgtgaggaac acatcggaca agaaataacc ttgaaaggat
     3001 gggttggccg tcgtcgtgac cttggtggtt tgatctttat cgatcttcgt gaccgtgaag
     3061 gaatcatgca gttggttatc aaccctgaaa aagtatctgc agaggttatg gcaacagctg
     3121 aaagccttcg tagcgaattt gttattgagg tgactggtca ggtcgctgcg cgtgagcaag
     3181 ccaatgataa gttgccaact ggtgcggttg agttaaacgt gacagctctt attgtgctta
     3241 acacagctaa gacaacacca tttgagatta aggatggcat tgaggcaaat gacgatacac
     3301 gtttgcgtta ccgttacctt gaccttcgtc gtccagaaat gttggaaaat cttaaacttc
     3361 gtgccaaggt gacccactct atccgcaact acttggatga gttggagttt atcgacgtgg
     3421 agacaccatt cctttctaag tcaacgcctg aaggggcgcg tgattattta gtgccgtctc
     3481 gtgttaataa ggggcatttt tacgctcttc ctcaaagtcc acaaatcacg aaacagctct
     3541 tgatgaatgc tggttttgac cgttactacc aaatcgttaa atgtttccgt gacgaggact
     3601 tgcgtggaga ccgccagcct gaatttactc aggtcgactt ggaaacgtcc ttccttactg
     3661 agcaagaaat ccaagatatt acagaaagct tgatcgcgcg cgtgatgaag gaaacaaaag
     3721 gcatcgaagt aacgctacca ttccctcgta tgaaatacga tgatgctatg gctctttacg
     3781 gttctgacaa gccagatact cgttttgaca tgttgcttca ggacttgaca gaagtggtcc
     3841 gaggtgtaga ctttaaagtc ttttcagaag cacctgctgt aaaagcgatt gtggtcacag
     3901 gagctgcgga caactattca cgtaaagaca tcgacaagat gacggaagta gccaaacagt
     3961 atggtgccaa aggtcttgct tgggtcaagg tggttgatgg agaattaaac ggaccagttg
     4021 ccaagttctt gactggtatc caagaagaat tgacaacagc gcttgttctt gaagataagg
     4081 acttggttct ctttgtggcg gatacgcttg aagtggctaa tgcaacactg ggtgcccttc
     4141 gtggacgtat tgctaaagag cttggcttga ttgataatga taagttcaac ttcctttggg
     4201 tggttgactg gccgatgttt gaatggtctg aagaagaagg ccgctacatg agcgcccacc
     4261 atcctttcac acttccacag gaagagactg ctcatgaatt agaaggtgat ttggctaagg
     4321 ttcgtgccat tgcttacgat atcgtcttga acggttatga gcttggtggt ggtagccttc
     4381 gtatcaacca aaaagacctt caagaacgca tgttcaaggc tcttggtttc tcaactgaag
     4441 aagccaatga ccagtttggt ttccttcttg aagccatgga ctatggtttc ccaccacacg
     4501 gtggtttggc tatcgggctt gaccgttttg tcatgttgct tgctggagaa gaaaatatcc
     4561 gtgaagtcat tgcctttcct aagaacaaca aggcaactga cccaatgaca caagctccat
     4621 caacagtcgc tctcaaacaa ctagaggaac tcagcttaca agtagaagaa gatgaaacaa
     4681 acaaaacgaa ttaagcggtg gcgctattat ctgcgccgct ttgcttatca gataaaaatt
     4741 ttacgtgtct tacaaagtat ctctcgagaa aagtatgatg agaagatttc ggcctctctg
     4801 gtctatggtt ttttatcagc agtagcagtt aatttctttt tccaaccagg gcatgtgtat
     4861 tcgagtggtg caacaggtct ggcacagatt atctctgcct tgagtaatca ctggtttggt
     4921 tttcatattc cgatttcgct aagcttctac gccattaact tccctttgat ggtcttagct
     4981 tggtatcaga ttggccataa gttcaccgtc tttaccttta tcacggtatc tatgagttcc
     5041 ttctttatcc agtttgtccc tgtggcaacc ttgacagagg atcccattat caattccctt
     5101 tttgggggtg ttgttatggg tttggggatt ggttttgctc ttcgaaacaa tatctccagt
     5161 ggtgggacgg atatcgtcag cctgactatt cgtaagaaaa cgggtaagaa tgtcggtagt
     5221 atttctttct tggtaaatgg aactatcatg ctgatagcag gtttgacctt tggttggaaa
     5281 tacgctcttt attctatgat taccatcttt gtctctagcc gtgtgacaga cgcagtcttt
     5341 actaagcaaa agcgtatgca ggccatgatt gtgacaaatc atccagagaa ggtaattgaa
     5401 aaaatccata aaaaattgca ccgcggagca accatgatcc acgatgcaga aggaacctat
     5461 aatcacgaga gaaaggcagt tttaatcact gtcattacac gtgcagagtt taatgaattt
     5521 aaacagatta tgacacaagt ggatccaagc tcctttgtct ctgtctcgga aaatgttcat
     5581 attctaggaa gatttgttga gatagataat tagtgattaa tactcttcga aaatctcttc
     5641 aaaccacgtc agcttcacct tgccgtaggt atggttactg acttcgtcag tttcatctac
     5701 aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgca ctttgatttt
     5761 cattgagtat aaaaagatct tgggaaaaga ctcaatttca gtagaaaagg aagtaaatct
     5821 tcccacaata aaacgcatag tatcaagttt tttcaatacc tgatagtatg cgtttttgct
     5881 tttaacgact ttttgcccag tctcttattt ttcgataatt ttgtgggcga tcaattgacg
     5941 gtaacaaatt tgtttattgt ttttattatc attaataatc tggagaatag tttcaaggga
     6001 aacacgacca agctctaaac tattgatatc gacataggct gccaagttga gcttgggatt
     6061 aaccgagtca aagctgagaa cagggacatc cagctggtgt ttggcaatat agttacaaac
     6121 accttcagct aggaggctat cggttgtgat gatagcatca atttgtggat cgtgcttgaa
     6181 taatcgcttg ctaaatttat agcccttttc ttccagaaac tcgtcggcaa agtagatgcg
     6241 attgttgtca gtggtaagtt tgtaatgttt aagcgcctgt tcatagcctg ttaaacggtc
     6301 tttggtcacg aagagctttt tacttcctcc gataaaggca atgcgtttgc agcctttttt
     6361 gatgaaatat tcagtcgcat caaaaccagc ttgaacattg tcgttgtcga caagtgggat
     6421 gaaaggagat agagatttac ctaagataag gaaggggaac tgttcttctg cgacgagttt
     6481 tacgagaggg tcttcttctt gggcatagag aaaaattagc ccatctacac gcttgccgta
     6541 gaccatttgt gaaatagcgt tgagacgctc cttctcatct ttccctgttg ctatctgaat
     6601 ggcatagtgg ttttcagatg cgacttgaga gatgccacgt agaaccgatg gaaagaaagg
     6661 attctggtag aaggcgtctg agtcatcagg aagaactaat ccgataacct gagtatagct
     6721 gcttaccaag ctacgagcgt tgaggtttgg gtggtagttg agttccttca tagctttgcg
     6781 aacacgtttt tttgtttcgt cgctaatggt tgatttattt tgaataacac gggttacggt
     6841 tgaaggcgaa acaccagcag ccttggccac gtctttaatc gtaacgggca taaaaatctc
     6901 ctatttatgg taatttggat cacggaaatg tgttttataa aagatagtga ccagatacag
     6961 aacaaaaaga atattttgta cagtaatcag ggttgtcata ttttggccaa ataatcccaa
     7021 aataagtgta atcagagtcg gcaatcctaa acagttcaag ataaaatggt agcactcttt
     7081 aaaggtatta aatgaaaaga ggcgtgatct tttggtgata taaaggagaa agctagctcc
     7141 aagagagacg ataaagaaat tcaaaccaaa gaggaagctc gcaccgagaa ctaggaagag
     7201 gctgatatag acacgatttt gttggtacca gtctttagaa attgcttggg tcaagctgtc
     7261 tttgcttttg aaactctcag tctgaatggc tcggtaagag atgcgggtca gttccttgct
     7321 ttccttgctg atgactagct catttgtatc gaaatgcagt tgcaagtcct taggtaattc
     7381 cttgatttga cttggaccaa tcacaacaga aggggcttga ctagctgttc cagtataagt
     7441 taatgtgcca tcgacaattg tagcatgttc agagagatcc tggacaacct tatctgtcag
     7501 aggttcatag acattatcga taaaagtttc tagcggatag gtctcctggg agctgttttg
     7561 gatagcaatg ggtaccatag acaagctgat aaggaagata ctggtaaaga gaagttgaaa
     7621 ccagttgagc tcgaaacgtt tggacagggg cttacgaacc ccccaaatac ttgaaaaata
     7681 ggaaaatgga tatggaagca taggtatctt tctaaaaggt gttttctata tgagtattat
     7741 tatatagaga aatgcgcttt atttcaagtc taggagaaaa attgcttgct acaaggatat
     7801 tatttacagt gacaattgct aataatcaat gaaaatcaaa gtgcaaacta gccacaggtt
     7861 gctcaaagca caactttgag gttggagata aagctgacgt ggtttgaaga gattttcgaa
     7921 gagtataaaa tgaaaaaggg tggcggggat aaattatccc ttgtcgccac cacttgtaag
     7981 tcctgaaaca aagttctttt gtaggaagaa gaagagaata cagattggaa gggcgatgag
     8041 gatagcacct gctgagaagt aggcaatctt caagtttttc gcattgttaa cgaaggtttg
     8101 gagacctacg gcaacagtaa agtattcttt ctcacgaagc aagaaactag agaggatgta
     8161 gtccccgaaa ggtcccatga aggcccagag agcttgtacg gcaaccattg ggcgaacaag
     8221 tggtagaaca atttgccaga agcggcggaa gtgtcctgca ccgtctagtt ttgcagattc
     8281 gtctaaagac attggcactg tatcgaagta gcctttcatg agccaagcat tcatcgggat
     8341 accaccacca acgtagagga agatgaggaa ccagttgtgg ttaagggcgt tcaacataag
     8401 cgccataacg aagaaggctg tcaaagcggc catagttggc accatttgga tgatcaagaa
     8461 gaagaccaaa ctttgtttac gagccaagaa gttgtaacgg ctgtaagcat aaccagcaag
     8521 tacgatgata cttgtttgaa cagccatggt aattaaggcg ataatcaaag tgttgaggta
     8581 ccaagtaccg tacaaggttt cagtgaagag gcctttaaag ttatcaaaat tgaggtcgat
     8641 attagtatct agtttaaagg ctgagacgtt acctgcttta aaggctgaca taatggtaat
     8701 caacagtgga tagataatta caattgatag accaatcagg taaaggtaag taaggctttg
     8761 agtcagtcta cgtttgagtt taattgagtt attcatctta gacgtcctcc atatcaaatg
     8821 cgtgtagttt cttgaatgcg atcatagaga ttgagatgac aatgatagag ataatcaagg
     8881 taacagctgc cgccattgag tattgaggag atgtacctgt tgtcaaacgg tagatccatg
     8941 agatcaagat atcggttgaa ccagctccac ctccgacact accaggtcct ccaccattga
     9001 agaggtacat gatagagaag ttgttaaagt tgaaggtgta ttggctaatc aaagtaggtg
     9061 ccgcaacagc caaaatcatt gggaaagtga tgttgcggaa tttttgccaa gcgttggcac
     9121 cgtcaatata agctgcttcg taaaggtcgt taggaataga ttgcaagata cccaaggtca
     9181 gaacgtagat gtatgggaat ccgagccaac cttgcatcat aatcaaggca atcttagtcc
     9241 aagttgggtc tgttttccaa ggaataagag ctccatcaag gaaaggaagg aatttagcca
     9301 agattggcaa tacttgagtg ttgatagcac cgacactatc gttaaacatg tttgagaatg
     9361 tcaagatagt gatgaaggct gggacagccc aaggaagaag gaaaataaca ccaaagatac
     9421 gttttccttt gataaatggt tggttggcaa tgatagctgt gaagatacca attacgattt
     9481 gtaaagtaga agctgccaaa gcccaaatga tagtccaaga aagaacagaa ccaaaggcag
     9541 aacggaaggt actcaagctc caaatgtttg taaagttggt caaaccaacc cagtccaaca
     9601 acttgtttgg tggcaagtgt tggaagtcgt agttggtaaa ggcgatcatc aaggttacga
     9661 taactgggaa gataatcgcg aaggtcatgg caacataaga tggaatgatc aagaggtaag
     9721 ggaagccatt ttcataaatc cctttgatca tgtctttgag tgtgcgtgga actggaattc
     9781 cattgttaat gcgttttgca atcgtatgtg catctttgat atttgagaaa taaaagagta
     9841 cataaacgat tacaaagatt agatggaagg caccacgaat cagcataaag agggaattat
     9901 cacgacctgg tttgtcacca agagtgatga ggttgctcaa ttcaggggtt gcaagtgcta
     9961 ggaagtaaag gacaaatacg atggttacac caaggaagat aaaacctttg gctttttgtt
    10021 tattgtaaat ctgtcctaac ccaggaatga tagacagcag ggctgcttta ctaggttgtt
    10081 gcttttccat attaactcct ttcataggat actacttttt tataagaaag ctaaattatt
    10141 tgtgtctcta ttgataaatt caaaggggga tttgatttcc accccccttg aacaaatttt
    10201 ttattcacca aatttttgtt tgattgtttc tttgatcaat gttacagcat cgttagcagc
    10261 tgttttagca tctttttgac cacttacagc atcaaagagc atatttttcg ctggatccca
    10321 aactgcagac atttgagaga tgtttggcag tggttgagtg ttcttgaact gtttgataac
    10381 agctgttgtc aactcatcgt ttttaccttc agcgtatgaa cgagcctcag tattagctgg
    10441 gatttcgtta gtcttatcat ataatacttt ttgttgttca gttgcaacaa ggaagtctac
    10501 aaatttttga gaagcttcaa ggttcttaac ggcttgagga atgacccaag ctttaccacc
    10561 accgaatgca gcatattctt ttccatttgg aagagttggg atagttgcaa ctccgtagtt
    10621 tactttagca tctttaaagg cttgagcttt ccaaggtccg tcgatgatag cagctgtttt
    10681 accttcttgg aattgagttt ggattaagtt tccagcacct tctgtatctt gcataccttt
    10741 aggccatttt tcgtaccaag atttagcgta gttgatacct acgatagaac cgtcgtttgc
    10801 aagaccgatg tctttagcgt ctttaccgtt ttggccaaag acgtaagcac cgttaccggc
    10861 aagaagtcca tatgtatagt agaagtttgt ccagtcagct aggaaggcag tagttttacc
    10921 atcttcacca gcgaatgcgt atttgctatc tttagcaagg ttttccaagt cagcaaatgt
    10981 ttttggagca tctttcacca agtctttgtt gtagtacata acaagtgact cgataacggc
    11041 aggagcaccg taaactttac cattagcagc tgttacaaga gatttagttg tgtcgtctgt
    11101 tttagcacca tcgctcaatt tcacttctga aagttgtccg tcagaaccaa ggctacctac
    11161 acggtcgtat ggagccatca taacatcagg gacattacca gattggttgt caagagaaag
    11221 tttatcaaga cctcctagag catcaccagt tttaagagtg acttttactc cagcttcttt
    11281 ttcataagct ttagcaacct cttcaatata gctcttatat ccctcgtcta catatacagt
    11341 gagttctttg acttcagatg aaccagaatc agcaggctta tcagcagttt tgcttccgca
    11401 agctaccaaa agcaagctag caagtgtagc agttccaagc accgcagcgc tcttcataaa
    11461 tttagatgac atagtgtatt cctcctaaag aatagcaagt tttattgata aggaaacgca
    11521 aacgttttcc tttatgagct tagtatagca caaatagaaa acggttgcaa gtattttttg
    11581 taaaaatttt aaaaaatttt taagcttgat ttgatagcat aattttgcat tttaatagaa
    11641 gaaaagtatg aaatagataa gaatacagta tttttaataa aaatatatga aatctgtcta
    11701 aagagggaaa tcaggagggg ctaccttcct atttctaatc tatatctatt ttctatagac
    11761 gtttgtgctt tgttgcatat aaattcaagc tccctaagga ggttttgtac tttgtatact
    11821 gtctcgtgag agtcaaactt taggattaga tagttttaag ttatctttgc taaaatgagt
    11881 tagtctcctt aaaaataatc aaaaaagttt tttaaaaacg cttgcaatta tgcttgaaaa
    11941 ggagtatact tataagtaac gcaaacgttt gcgtctgaaa aatacgcaac gttccattat
    12001 tttaacacac gaggtgctat tatgaaaaaa cgtcaaagtg gtgtgttgat gcacatctct
    12061 tctcttccag gagcttacgg aatcggatca tttggtcaaa gtgcttacga cttcgttgat
    12121 ttcttggtcc gtacaaaaca acgttactgg caaatccttc cattaggagc aactagttac
    12181 ggggattctc cttaccaatc tttctcagcc ttcgcaggaa acactcattt tatcgattta
    12241 gatatcttgg tggagcaagg tttgttggaa gcaagtgacc ttgaaggagt tgactttggt
    12301 agcgatgcgt ctgaagttga ctatgctaaa atctactatg cacgtcgtcc tcttttagaa
    12361 aaagcggtga aacgtttctt tgaagtcgga gatgttaaag attttgagaa atttgctcaa
    12421 gacaaccaat catggcttga gctctttgct gagtatatgg ctatcaaaga gtattttgac
    12481 aatcttgctt ggactgaatg gccagatgca gatgctcgtg ctcgtaaagc ttcagcactt
    12541 gaaagctatc gtgagcaatt ggcagacaag ttggtttacc accgtgtgac tcaatacttc
    12601 ttcttccaac aatggttgaa attgaaagct tacgctaacg acaaccacat cgaaatcgtt
    12661 ggggacatgc caatctacgt agcggaagat tcaagtgata tgtgggcaaa tccacatctc
    12721 ttcaaaacag atgtcaatgg taaggctact tgtatcgcag gatgcccacc agatgagttt
    12781 tctgtaactg gtcagctttg gggtaatcca atctatgact gggaagcaat ggacaaagac
    12841 ggctacaaat ggtggattga acgcttgcgt gaaagcttca aaatctacga tatcgttcgt
    12901 atcgaccact tccgtggctt cgaatcttac tgggaaatcc ctgctggttc cgatacagca
    12961 gcacctggtg agtgggtgaa aggtccaggt tacaagcttt ttgcagccgt taaggaagaa
    13021 cttggtgagc taaacatcat cgcagaagac cttggcttca tgacagatga agtgatcgaa
    13081 ttgcgtgaac gtactggctt cccaggaatg aagattcttc aatttgcctt caacccagaa
    13141 gacgaaagca ttgatagccc acacttggca cctgctaact cagttatgta cacaggaaca
    13201 cacgataaca atacggttct tggttggtac cgtaatgaga ttgatgatgc gactcgtgag
    13261 tacatggctc gttacacgaa ccgtaaagaa tacgaaacag tggtacacgc tatgcttcgt
    13321 acagtatttt catcagttag ctttatggca attgcaacta tgcaagattt actagaattg
    13381 gatgaggcag ctcgtatgaa cttcccatct acccttggtg gaaactggtc ttggcgtatg
    13441 actgaagatc aattgacacc agctgtcgag gaaggtttgc ttgacttgac aacaatttat
    13501 cgccgaatta atgaaaattt ggtagattta aagaaataag acaataatca ggagacaact
    13561 aaacatgtta tcactacaag aatttgtaca aaatcgttac aataaaacca ttgcagaatg
    13621 tagcaatgaa gagctttacc ttgctcttct taactacagc aagcttgcaa gcagccaaaa
    13681 accagtcaac actggtaaga aaaaagttta ctacatctca gctgagttct tgattggtaa
    13741 actcttgtca aacaacttga ttaaccttgg tctttacgac gatgttaaaa aagaacttgc
    13801 agctgcaggt aaagacttga tcgaagttga agaagttgaa ttggaaccat ctcttggtaa
    13861 tggtggtttg ggacgtttgg ctgcctgctt tatcgactca attgctactc ttggtttgaa
    13921 tggtgacggt gttggtctta actaccactt tggtcttttc caacaagttc ttaaaaacaa
    13981 ccaacaagaa acaattccaa atgcatggtt gacagagcaa aactggttgg ttcgctcaag
    14041 ccgtagctac caagtaccat ttgcagactt tactttgaca tcaactcttt acgatattga
    14101 tgttactggt tatgaaacag cgactaaaaa ccgcttgcgt ttgtttgact tggattcagt
    14161 tgattcttct attattaaag atggtatcaa ctttgacaag acagatatcg ctcgcaactt
    14221 aactctcttc ctttacccag atgatagtga ccgtcaaggt gaattgctcc gtatcttcca
    14281 acaatacttc atggtttcaa acggtgcgca attgatcatc gacgaagcaa tcgaaaaagg
    14341 aagcaacttg catgaccttg ctgactacgc agttgtccaa atcaacgata ctcacccatc
    14401 aatggtgatt cctgaattga ttcgtctttt gactgcacgt ggtatcgatc ttgacgaagc
    14461 aatctcaatt gttcgtagca tgactgccta cactaaccac acaatccttg ctgaagcgct
    14521 tgaaaaatgg cctcttgaat tcttgcaaga agtggttcct cacttggtac caatcatcga
    14581 agaattggac cgtcgtgtga aggcagagta caaagatcca gctgttcaaa tcatcgatga
    14641 gagcggacgt gttcacatgg ctcacatgga tatccactac ggatacagtg ttaacggggt
    14701 tgcagcactc catactgaaa tcttgaaaaa ttctgagttg aaagccttct acgaccttta
    14761 cccagaaaag ttcaacaaca aaacaaacgg tatcactttc cgtcgttggc ttatgcatgc
    14821 taacccaaga ttgtctcact acttggatga gattcttgga gatggttggc accatgaagc
    14881 agatgagctt gaaaaacttt tgtcttatga agacaaagca gttgtcaaag aaaaattgga
    14941 aagcatcaag gctcacaaca aacgtaaatt ggctcgtcac ttgaaagaac accaaggtgt
    15001 ggaaatcaat ccaaattcta tctttgatat ccaaatcaaa cgtcttcacg agtacaaacg
    15061 ccaacaaatg aacgctttgt acgtgatcca caaatacctt gacatcaaag ctggtaacat
    15121 ccctgctcgt ccaatcacaa tcttctttgg tggtaaagca gctccagcct acacaatcgc
    15181 tcaagacatt atccatttaa tcctttgcat gtcagaagtt attgctaacg atccagcagt
    15241 agctccacac ttgcaagtag ttatggttga aaactacaac gttactgcag caagtttcct
    15301 tatcccagca tgtgatatct cagaacaaat ctcacttgct tctaaagaag cttcaggtac
    15361 tggtaacatg aaattcatgt tgaacggagc tttgacactt ggtactatgg acggtgctaa
    15421 cgtggaaatc gctgagttgg ttggagaaga aaacatctac atcttcggtg aagattcaga
    15481 aactgttatc gacctttacg caaaagcagc ttacaaatca agcgaattct acgctcgtga
    15541 agctatcaaa ccattggttg acttcatcgt tagtgatgca gttcttgcag ctggaaacaa
    15601 agagcgcttg gaacgttttt acaatgaatt gatcaacaaa gactggttca tgactcttct
    15661 tgatttggaa gactacatca aagtcaaaga gcaaatgctt gctgactacg aagaccgtga
    15721 cgcatggttg gataaagtca tcgttaacat ttctaaagca ggattcttct catctgaccg
    15781 tacaatcgct cagtataacg aagacatctg gcacttgaac taatactctt cgaaaatctc
    15841 ttcaaaccac gtcagcttta tctgcaacct caaagcagtg ctttgagcaa cctgcggcta
    15901 gcttcctagt ttgctctttg attttcattg agtataagat acaaatttat actaatacat
    15961 tttgtaaaaa agcgagtttc gattgaaatt cgctttttta atgatgtaga tttgggtcaa
    16021 tcttgtctaa aaatagggaa atcctagata cagtgaaggc tttaaatgct ggtttttact
    16081 gtcctcagcc ttatattttt tcgtagttgg ttacctcata tctattatat tcgcttacat
    16141 aaagtattat aatataattg taggaaagaa ggtgttttta tgatatacac acttaaattg
    16201 gtgttgttta ttacctttct tgtaataagc ttgttacctg ataagatttt tggaaaaaat
    16261 aaaaaaattt ggaaaatagt ttttgcaata ttgacggcag tggcagcatt gtcatttatg
    16321 tactaagtta ttttaagaat gtagggaaat aaaccctaca ttctttttag ttttttctgt
    16381 tttctaaatt ctatttatcc aagcgattca acatttcttg cttcttcgct tcaagttctg
    16441 cacgcttttc ttcgatttcg gcatgttttt tctcgagttc agaacaactt gcaccattgc
    16501 taaattcttt tcgccatcag gagatagggt gagtcgacat gtctattact cacccaaagc
    16561 agtcctacaa agcaggaatt ttctgttact tttttggaaa tagtaacgtt tatacagctt
    16621 tgacacttcg tatcaaagcg ccaaacacac tccgaggggt ttacagaaag cagaaaagga
    16681 atgatctggt ataagatcat tccttttctc tctttttctt taagtaatta tatacaatgt
    16741 acgacgaagt cgtcattgca atgctgatcc accacctaaa gggaacttta aacaacattg
    16801 ataagataaa gaatataaac aacgaaaata cgttataccc aattaatttt attgtatatc
    16861 tcatgattaa aagttaatcc ttccgttgtt aggaatggca tcatttttat cccataattg
    16921 tgctaaataa gtccccggtg ataataaatt catagcgaat tctaaagcaa catcatttac
    16981 aaaccaacta cctagatatc tagaaattgc tgaacgaata gcactttttg ctgcatgttt
    17041 tccttttact ttaattagat ttgcaaggcc tgcagtagtt cctcctaatg ctaaagctat
    17101 tgcagtatct aatagagcac ccatttgatt aactgtaata ccttgccaaa ctgctctaaa
    17161 tggagagtat gtaggtggga ttgtataatc gccttgtaat tgtcggttaa ttacttcttt
    17221 gatccattgt tgtgagacgt ctggatgaaa agattggatt tcgtttgcaa gtgtattgat
    17281 ttgttcttct gttagagaag tgacaggttg aagttccata tttgtttcaa tttgtgatac
    17341 ttgttcagaa gcgtatacag ctgaaacact tggaatcgct gatacaatta acacaattga
    17401 cgtcaaaaaa accgaaataa atttcattaa tttgttcatg agcttttctc ctttttattt
    17461 gcatctgctt acattttatc atatactgtt attatagtca aaaaaatatg ctattatgtt
    17521 aaaaaaatat ttttcaaaat ataaatggac ggatttattt tggattttat ttgttatttt
    17581 gacctgcctc tatattggta accatgattt gtttactctc aatcatcaag aattctcttt
    17641 tcgtggtagc gtttggggtc tggtactggc cttatatcac ttactattca ttgataagtt
    17701 tgttatatcg aatcgaaaat aaagattaga gctatgcttg actgtgtact tttaggattt
    17761 attttggagg aagattttgt ctctattatt tattatttta aatttattta ttttgtataa
    17821 gatctattct ttgaggcggg aaaaatcgaa atacttgatt tatacggcct atatcatatt
    17881 tggggtaaat gtagtatatg gtattcaatg gttattaaaa gaactgattt caactatttc
    17941 cccttaatgt gtatggaaac tataacaaaa cgcataagat taaggtttgt gtacctgttc
    18001 ttatacgttt ttgtcatatt gaagattttc ggtaagtttt ctacttttgc gaagtctagt
    18061 ttatactcaa tgaaaatcaa agagtaaact agccgtaggt tgctcaaagt acagctttga
    18121 ggttgcagat aaaactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac
    18181 tgacgaagtc agtaaccata cgtacggcaa ggcgacgttg acgtggtttg aagagtatta
    18241 gaatgctttc ccaaccaaaa tctctgcttc gatggtaatc tctgtcagtt tgctccagtc
    18301 aattttgctc tggtcaacgt gaaagaggag aggagtcata ctgagtaggg cttggagttg
    18361 ttctgctgtg atagtcttag tcagagaggc agtttgacta gataggatgg taaagtgttc
    18421 ctggaaatgc tccttgatat cttggttaga atactccttg tttgtcagct ggtcctgtac
    18481 tctttgacgg atttctttga ggtgattttc agttgggata acctttatca agataccgtc
    18541 tttggataaa acgcgacgaa attctccata gttggcaggt gagaagatat caagcagaat
    18601 atccatgtta gcgtctttta taggaagtcg tgccaagtcg ccaacgaacc aattgactgc
    18661 ccagttgggt tcacttttag cagcgatttg gactgaatct ttggagatgt caaaggcata
    18721 gaaagttttt tcagagtgac tttcttgtag tttgcgagaa tagaatcctt caccacaacc
    18781 gatatccaaa attgttgtgg tagtttttga gcttgcaagc aagtcagata cagcatctaa
    18841 gatagcttgg taaaagccgg cttctaggat ttgttgacgg ttttgaaaat tttccttgtc
    18901 gtagttagca gattgcttga tttgagggac tagattgaca tagccaaatt tcgccaagtc
    18961 aaaagaatga cggttgcagc acttgaaatt agtctctaac agagtcagat tttcttgaca
    19021 gataggacag gcaaaggcag tcgcagaagc aaaacgctga agtttgggct tgagatttgt
    19081 attcatagtt taagtgtatc aaaagaggca aatgaaagca actttaggaa taagtagatg
    19141 ggagattcag tagaaataaa actaattctc caatttatag aatgaaattg cttttatatc
    19201 taaattgcta tataataatg ataaggagga aataagtatg ttaaaagaag tattaaccgt
    19261 tgcaaaagtt gcgaaaaaat cttcattatt tttgggtggt gtcgcatttg gtacccttgg
    19321 tttgaaaatc ttagcaagta aggaagctaa aaaaggttat tctaaagctt tggctaaggc
    19381 ttacaagttg aaagacgagc tagatgcatc tgtttctgtt gtgaagcaac atggagacga
    19441 tgtcttgcaa gatgccaaat atttgtacga gcaagagaaa aaagaagagc aattagatag
    19501 ccttataggt gaataatatg tcttttaaag tgctacatag aggataccaa catatccgac
    19561 tatcatcttc tttttcactt accttggata ttcaagacta tcttcgttcc ttggcgagag
    19621 atgaaaaggg gattgagtct atccagtttt acatggatca acagcacttt actctacgca
    19681 taaaagaagg cttttctgta ttagataatg cagaagcctt tttaaaaaga attgataaag
    19741 ggaaagtttc tgagttgatg actcttccca ttcgtagaga agagagtgct tattctattg
    19801 tttcaggtgc agcggttaag cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct
    19861 atatatggac ttgttatcag gctttgggtt atattagaga agcctatcaa acactagcgc
    19921 gtaaggaact aacgatggaa gtcttggact gttcggctat tttattgtct ttgtttatga
    19981 accaatccaa gacagctagc aatatcatgt ttatgcttga tttggggaat catttagatc
    20041 agtggtcctt gaaaaaaact gcaacagatt tagaacagag tcttcttgca aaagagagcg
    20101 atgtattcct agtacagggc gatacggttg ttagtatcaa gagttccgat gttcaaatag
    20161 gagatgtctt gatcttatct caaggaaatg aaattctgtt tgatggacaa gtagtttcag
    20221 gtttaggtat ggtcaacgaa agttccttga caggagagag ttttccagtt gaaaaaagag
    20281 agtctgattt ggtttgtgca aatacagtat tagaaactgg agagttacgc attcgtgtaa
    20341 cagataatca gatgaacagc cgtattttac agctgattga gttgatgaag aaatctgaag
    20401 aaaacaagaa aacgaaacaa cgctatttca tcaagatggc ggataaagtc gtcaaatata
    20461 atttcttggg ggctgggctg acttacctat tgacaggttc tttttctaag gctatttctt
    20521 tcctattggt cgatttctcc tgcgctttga aaatctctac tcctgtagct tatttgacag
    20581 ttatcaagga agggttgaac cgtgaaatgg tgattaagga tggagatgtt ctggagaaat
    20641 atctggaagt tgatactttc ttgtttgata agacaggaac aatcacaact agttatccta
    20701 tagttgaaaa ggtgttacct tttggagact atagtgagga agatattctc agaatcagtg
    20761 cctgtcttga ggaacacatt tatcatccta ttgctaatgc catcgtcaag caagctgaga
    20821 tagagggaat tgaacatgag gaaatgcatg ggaaactcca atatatcgca agcaagggga
    20881 tcaaatctca tatagatggc caaccagttc ttattgggaa ttatgtcttg atgcaggatg
    20941 agcagattca tatcagttca gaacaaaatg ctttaattga agagtacaag agtcactaca
    21001 atctcttatt cttggcttat caaaatgaat tgattggaat gttctgcatt catactcctt
    21061 tgagaaaaga agcaaaaaca gccttggata aacttaaggc acaggggaaa aaattgattc
    21121 tggcaacagg agacaccttg attaggacag aggaattagt caaagatttg ccctttgatc
    21181 aggtctatac agacttgaaa cctgatggga aatttgagtt agtagagaaa ctgcagaaag
    21241 caggtcacac tattttgatg gttggagatg gattgaatga ctcagcggct ttaactctat
    21301 cagatatcgg tgtggtgatg aatgagagtg cagatatttc taagcagatg agtgatatct
    21361 tattgttaga taatcgtttg gatttcttcc aagagttgga ttcgttatca tcatctttgc
    21421 aaacactcat caagaaaaat attcaggata ccgttgtcgt aaatagtagt ttgattggct
    21481 ttggcttatt taattggttc agtccttcaa atctctctat cttacataat ctaacaacct
    21541 tgcgcattgt cctgcgtagc ctgtctatta aaaatagata gatgagagtt attaacagca
    21601 aaaaggagtt acctttctcg aggttaactc ctttgtttat agataaatgt tgaatagttt
    21661 agtaagtcaa tacaaagtat tttttcttcc cacgacggat aacagtcagt tcattctcta
    21721 acttatcagc gtcactcaag acatagtcaa gctcttggat gcggtcgccg tttacgtaga
    21781 tggctccgtt ttggacgtct tcacgggctt ggcgttttga gttaactata ccagatgaga
    21841 cgagcagttc cacgatattg ttgttttcgt ctgcctgtac ttggtagttg ggcacaccac
    21901 gaagtccttg tttgagctct ttgacagaaa ggtttttgat gtttcctgca aagagttgct
    21961 cagtgatgtt aagtgcttct ttgtaggctt cttctccgtg aacaagtgta acaacttcac
    22021 gagccaagac tttttgagcc aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat
    22081 cttcaatctc atcaagtgac aagaaagtaa agattttcaa gaagcgaaca gcgtcagcgt
    22141 ccatcacgtt catccagaat tggtacattt cgtatggaga agtcttttcg ggattgagcc
    22201 agacggcatt tccttctgat ttaccaaatt tcttaccagt tgcatctgtg attagtggaa
    22261 cagtgataac gtgaccagtc ttgtccgcct tacgacgaag caattcggta ccagctgtca
    22321 tatttcccca ctggtcagaa ccaccgattt gaagagtgac attatggtct tggttaagga
    22381 cgaagaagtc atacccttgc atgatttggt aagcgaactc agtgtaagaa attcctgttt
    22441 cgatccgttt tttaacagat tccttactca tcatgtagtt gaccgtgaag tattttccaa
    22501 tatcacggag gaagtcaatg aagctgatgc tgccaaacca gtcgtagttg ttgaccatga
    22561 cagccttgtt ttcgccattt tcaaagtcaa gaaaacgaga aagttgtcct tggatagact
    22621 tgacccagcc atctactgtg tcttttgttt ggagactacg ttcagcatct ttgaaggacg
    22681 gatctccgat gagacctgta gcaccgccaa cgagcgcata aggtttgtga cctgctagtt
    22741 gcaagcgacg acttgtcaag attgcgacaa ggtggcctag gtgaaggctg tcagcagttg
    22801 gatcgtagcc agtataataa gaaacttgac cttcttctag ggctttacgc aaagcttctt
    22861 catcagtcgt ttgaaatatc aaaccacgct cttttagctc atcaaaaatg tgcatgtgtt
    22921 ttttctcctt tttaaaatta ttgtttctac ctattgtatc acaaacttgg gcaatagcct
    22981 agcagaatag ggaagaaagt ggctatttta ttgaaaattt cccttttgtg gtataataga
    23041 taaagtgagg aaatccatgc aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt
    23101 tttccagcaa aaacaaaaaa ataaaaaatc agctagacct ggcaagaaag gttcaagtac
    23161 caaaaaatct aaaaccttag ataagtcagt cattttccca gctattttac tgagtataaa
    23221 agccttattt aacttactct ttgtactcgg ttttctagga ggaatgttgg gagctgggat
    23281 tgctttggga tacggagtgg ccttatttga caaggttcgg gtgcctcaga cagaagaatt
    23341 ggtgaatcag gtcaaggaca tctcttctat ttcagagatt acctattcgg acgggacggt
    23401 gattgcttcc atagagagtg atttgttgcg cacttctatc tcatctgagc aaatttcgga
    23461 aaatctgaag aaggctatca ttgcgacaga agatgaacac tttaaagaac ataagggtgt
    23521 agtacccaag gcggtgattc gtgcgacctt ggggaaattt gtaggtttgg gttcctctag
    23581 tgggggttca accttgaccc agcaactaat taaacagcag gtggttgggg atgcgccgac
    23641 cttggctcgt aaggcggcag agattgtgga tgctcttgcc ttggaacgcg ccatgaataa
    23701 agatgagatt ttaacgacct atctcaatgt ggctcccttt ggccgaaata ataagggaca
    23761 gaatattgca ggggctcggc aagcagctga gggaattttc ggtgtagatg ccagtcagtt
    23821 gactgttcct caagcagcat ttttagcagg acttccacag agtcccatta cttactctcc
    23881 ttatgaaaat actggggagt tgaagagtga tgaagaccta gaaattggct taagacgggc
    23941 taaggcagtt ctttacagta tgtatcgtac aggtgcatta agcaaagacg agtattctca
    24001 gtacaaggat tatgacctta aacaggactt tttaccatcg ggcacggtta caggaatttc
    24061 acgagactat ttatacttta caactttggc agaagctcaa gaacgtatgt atgactatct
    24121 agctcagaga gacaatgtct ccgctaagga gttgaaaaat gaggcaactc agaagtttta
    24181 tcgagatttg gcagccaagg aaattgaaaa tggtggttat aagattacta ctaccataga
    24241 tcagaaaatt cattctgcca tgcaaagtgc ggttgctgat tatggctatc ttttagacga
    24301 tggaacaggt cgtgtagaag tagggaatgt cttgatggat aaccaaacag gtgctattct
    24361 aggctttgta ggtggtcgta attatcaaga aaatcaaaat aatcatgcct ttgataccaa
    24421 acgttcgcca gcttctacta ccaagccctt gctggcctac ggtattgcta ttgaccaggg
    24481 cttgatggga agtgaaacga ttctatctaa ctatccaaca aactttgcta atggcaatcc
    24541 gattatgtat gctaatagca agggaacagg aatgatgacc ttgggagaag ctctgaacta
    24601 ttcatggaat atccctgctt actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt
    24661 caagggttat atggaaaaga tgggttacga gattcctgag tacggtattg agagcttgcc
    24721 aatgggtggt ggtattgaag tcacagttgc ccagcatacc aatggctatc agaccttagc
    24781 taataatgga gtttatcatc agaagcatgt gatttcaaag attgaagcag cagatggtag
    24841 agtggtgtat gagtatcagg ataaaccggt tcaagtctat tcaaaagcta ctgcgacgat
    24901 tatgcaggga ttgctacgag aagttctatc ctctcgtgtg acaacaacct tcaagtctaa
    24961 cctgacttct ttaaatccta ctctggctaa tgcagattgg attgggaaga ctggtacaac
    25021 caaccaagac gaaaatatgt ggctcatgct ttcgacacct agattaaccc taggtggctg
    25081 gattgggcat gatgataatc attcattgtc acgtagagca ggttattcta ataactctaa
    25141 ttacatggct catctggtaa atgcgattca gcaagcttcc ccaagcattt gggggaacga
    25201 gcgctttgct ttagatccta gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa
    25261 accagagaag gtttctgttg aaggaaaaga agtagaggtc acaggttcga ctgttaccag
    25321 ctattgggct aataagtcag gagcgccagc gacaagttat cgctttgcta ttggcggaag
    25381 tgatgcggat tatcagaatg cttggtctag tattgtgggg agtctaccaa ctccatccag
    25441 ctccagcagt tcaagtagta gttctagcga tagcagtaac tcaagtacta cacgaccttc
    25501 ttcttcaagg gcgagacgat aattctctaa atgaagtggc caatcaatgg attgccactt
    25561 ttttcctttc gtgttacaat aagggtatga atcagtatca gaaaaagatt gttaacggaa
    25621 aaatttattc gctcctatcc ggcttaatat ggggaatctg tggaatttta ggagagtact
    25681 tctttactca ttatcaggtg tcttcgggct ggattacctc tatgcgtttg acactggcag
    25741 ggagtcttgt actcatttgg tctgcaatac aattaaaatc gcaagtgcta gatatttggc
    25801 gagacaagaa aaattacctg ccctttttag cctatgctat tttggggatt ttttcagttc
    25861 agtatttttt ctatctctgt gtagaatact caaatgctac gacagcaact attttacagt
    25921 ttattagccc tgtctttatc ctcttttaca atcgcttggt ttatcaaaaa cgagcgtcaa
    25981 aaagcgctgt tttctatgtt ttggttgcca tgctgggtgt ttgcttgatg gcgacaaagg
    26041 gagacctctc tcagttatcc atgacgccgc tagctcttat aacaggtttg ctgagtgcca
    26101 tgggtgttat gtttaatgtt atcttgcccc aaccttttgc taagcgttat ggttttgttc
    26161 ctacggttgg gtgggggatg attttggcag gtttgtttag caatgtcctc tcgccggttt
    26221 atcagctttc ctttactctt gatatttgga gtatcttgat ttgcctcatt atcgctttct
    26281 ttggaacggc ttttgctttt ttcatttcca tgaaggctgt gtccttggtt tctcctttgg
    26341 tggttgccgt tatcagtgcc agtgaacctc tctcttctgc tctcttgagt gttttgttct
    26401 taggattagt agtggattgg tccctccttc tagctatagc cttgattatt ttacccatga
    26461 tatttttgtc tatagaagaa gcgaaagaaa gtagataaaa agtcttttct taattctaaa
    26521 agtgtgctat actggaatag tcaataaaac acggggagat agttgtgaag agtgttttta
    26581 ggttgtatcg gtaggggctt gcttttaccg acagcacgtt ttatctcaca tccctaaaaa
    26641 atcataccta aaaacagaca aaaaggtttc aaatttgagg ccttttttgg tagaataggt
    26701 atcattataa cgaaccagga ggcacctatg actgctacaa aaatgaacgc tcaagaaatt
    26761 atccaattta tcgccaatgc tgaaaagaaa accagtgtca aagtaacctt tgaggggcaa
    26821 ctcgcaactg ctgtgcctag ctctgttgtc aaactaggaa atgtcctatt cggagactgg
    26881 aaggatgtgg ctccgcttct tgaaggtttg gtagaaaatc aagattatgt tgtcgagcaa
    26941 gatgctcgta attctgcagt tcctttgcta gataagcgtg ctatcaacgc tcgtatcgag
    27001 ccaggtgcga ttatccgtga ccaggtggaa attggtgaca atgctgttat catgatggga
    27061 tctgttatca atatcggtgc tgaaatcggt gctggaacca tgattgacat gggtgccatc
    27121 cttggtggcc gtgccatcgt tggaaaaaat agccacgttg gtgcaggtgc agttttggca
    27181 ggtgtgattg agccagctag tgctgaacca gtccgtgtcg gagacaatgt tcttatcggt
    27241 gctaatgcag tggttatcga aggagtccaa atcggtagtg gttcagttgt cgcagcagga
    27301 gctattgtta cccaagatgt cccagaaaac gtggtagtag caggtgttcc agctcgtatt
    27361 atcaaagaaa ttgatgccca aactcaacaa aaaacagcgc tagaggatgc gcttcgtacc
    27421 ttgtaattgt aaaagtaaaa aagaggcgga accctttttc cagcctcttt ctgctatatc
    27481 ggaggacaga tagatgttag atttgattca gactagacga gatttacacc agattccaga
    27541 gattggcttg gaggagttca agactcaggc ttatttgctg gatgtgattg agaaattgac
    27601 tacgggcaag gattttgttc aaattcgtac ttggcggaca gggattttgg tctacctgca
    27661 gggaagtcag ccggagcgaa ccattggttg gcggacagat attgatggcc tgcctatcgt
    27721 cgaacaaaca ggtctacctt ttgcttctca acaccaaggt cgcatgcatg cctgtggcca
    27781 tgattttcat atgaccattg ccttaggctg tcttgagcgc gcccttgagg agcaacccaa
    27841 gaataatctg ctctttctat ttcagcctgc tgaagaaaat gaagctggtg gtatgctcat
    27901 gtatgaggac ggtgcttttg gagattggtt gccagatcaa ttctatggtc tccatgttcg
    27961 tccagatttg aaagtcggcc agattgcgac taatactcat acactctttg caggaacttg
    28021 cgaggtgaag atccgtttca aaggcaaagg tggccacgca gcttttcctc atgaagccaa
    28081 tgacgccttg gtggcggcta gttactttgt gacccaagtg cagtcagttg ttagccgcaa
    28141 tgtcaaccca atcgagggag cagtggtgac cttcggcgtt tttcaagctg gaaccaccaa
    28201 caatgtcatc acagacacag cttttttaca tggaactatt cgtgccttga cacaggacat
    28261 gagtctcttg gtgcaaaaga gggtaaaaac agttgcagaa ggggttgcag ctgcctttga
    28321 tatggaagtc gaagtggaac tcaaacaagg tggataccta cctgttgaga acaatccagc
    28381 cttggcgcgt gaactgatgg acttctttga cgaaaaagac ggaatcgagt tgattgatat
    28441 cgaacctgct atgacaggcg aggactttgg ttatctcctt tcaaaggttg atggcgtgat
    28501 gttttggcta ggtatcgata gtccctacgc tcttcatcac cctcaaatga gtcctaagga
    28561 agaagtctta gctattgggg tggctgcggt gtctagtttc ctgaaaaaga aggcagcaga
    28621 atagagggct tgtctatgaa atcggaatta cgcaagcaag tcttgcatga aatgaaggct
    28681 atatctcaag agcaaaaaca ggctatagac caagctttaa ccgagcggct tttacaacac
    28741 cccttttatc aagaagccaa ggtcatcgca acctacctct ctttttctca tgagtttcaa
    28801 acgcaggaac tgattgagca ggcgctgaag gacggcaaga aggttttaat acccaaaact
    28861 tatcccaagg ggcgcatgga ctttgtggtc tatgatccgc aacagttggt aaaaactttc
    28921 tttgccttac tggagctaca gggagatttg gaagtggtgg atgcatctca gattgatttg
    28981 attcatgttc ctggtctggc ttttacgacg aaaggatatc ggataggata cggtggaggc
    29041 tattatgacc gctatctgga acatttttct ggtcgtactt tgagtacgat atatccttgt
    29101 caaattcagg actttatacc tgaaaaccat gatattcctg ttcaggaggt attaattgat
    29161 gaaggaaatc tttgatagac gttaccctgt gacgagtttc ttcctcttag tgacggcctt
    29221 ggtattttta ctaatgttgg tcactgcagg cggaaacttt gacagggcag atacattatt
    29281 tcgatttgga gccatgtatg ggccagctat tcgcctcttt cccgagcagg tttggcgtct
    29341 cttgtctgcc atttttgttc atattgggtg ggaacatttc attgttaata tgctttcact
    29401 ttattatctt ggaaggcagg tagaggagat tttcggttct aagcagtttt tctttctcta
    29461 tcttttatca ggaatgatgg gcaatctctt tgtttttgta tttagtccta aatccttagc
    29521 agcaggcgcc tctacctctc tttatgggct atttgccgcg attattgttc ttcgctatgc
    29581 aactcgcaat ccttatatcc aacagctagg gcaatcctat ctgacacttt ttgtggttaa
    29641 cattattgga agtgttctga ttccaggaat cagcctagca ggccatatcg gtggtgcagt
    29701 tggtggcgca tttctagcag ttatctttcc agttagagga gaaaaacgga tgtataacac
    29761 cagccagaga ttaggagcgg tagtcttgtt cgtaggactc gccattttgc ttttctacaa
    29821 gggaatggga ttgtgacgac taatactcaa tgaaaatcaa agagcaaact aggaagctag
    29881 ccgcaggttg ctcaaagcac tgctttgagg ttgtagatag aactgacgaa gtcagctcaa
    29941 aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacc tacggtaagg
    30001 cgatgctgac gtggtttgaa tttgattttc attgagtata atatgtaaac ttgaataaga
    30061 aaaaagctac ttaaaaaatg agtagctttt aatgtgtttt ggaaccattc gaaacaacac
    30121 agctctaaaa tagttattag cgttgactag gttctgcaat gccgccacca aagttcccaa
    30181 ggtaatttcc atcgtcatca tacacatctc cattattatg atctttgtag tagtatcttt
    30241 gatagccgtc agcaccagtg cctttatatc tgtagacatt atttgctccg ctgtttgaat
    30301 tgctagatga atattgttct gatgatccac catttgttga actatctcct gaaccagaat
    30361 aatcttgacc gccataatta tttgaatatc cattagtttg ttcagatgaa ctataatcta
    30421 catttgaatt ggaagatcca ttataagact cattagaagc actaccagaa gaattacttt
    30481 gactagaaga ttcctttgct ttttcagcag ctttttcagc agctttttct tcttctttct
    30541 tcaaaagttc gatttgtttt tgatatttat caacaagatc atttagagat ttaagagttt
    30601 cttccaaatt ctcttgttta aaagtttcat caactttttt gataaaatca atttgttttg
    30661 taatttcttt tacatttgtt tcaagagttg ctttatcttt aaatttgtca tctgaatctt
    30721 taacatcata agccaaacct ttttgcaaat gaaaatctac aattgcttta gatgattctt
    30781 tgactttgtt attaagatag tctttacctg catcggtttt aagtgaagtt tcagatagtt
    30841 cttttaattt agaaatagat tcatctgaag atgcatcaaa tgattcaaat agtttatcaa
    30901 cttgttcttt gtctgatttg ctatcttttt ctaattgtac aatttttgct tgttgttctg
    30961 cttgttgttg gttatatgaa taccatccaa accctaatac caatacaaca ccaactgcaa
    31021 ctgatgaaat gattactttt ttattgtttt cgataaattc tacaaatttg ttctccataa
    31081 tctttttctt tgatagtgtc cgcaattact ttaaaattaa aattacacct atctttctcc
    31141 cttattatat ttttattaat agcttccaca aaattaaaca acttatgttc ggctcggttg
    31201 atttaattta accatgacta ctccaacagt taagtcaaat ttaaaattat ttattatttt
    31261 ggaaccatta gaaacaacat agcaaagtta aaataaggct ttgtccgtac acaacttgaa
    31321 aaagtgcgca ccgattcggt gcttttttgt tttactatac taatttattc tactcttatt
    31381 ttaatgaaaa aacaaataca agtatagcgt tttcattagt ttttattaaa taggtagatt
    31441 caagtagaga atttacttaa tttatgtgtg gctaatcttt atataaatga ttttgttatt
    31501 ccttctcagt caattctttt ccaagttgga tgaggtaatt cttcaaatca tctttgactt
    31561 gtgggtgttt gagggcgtag tcgatggatg ttttcatgaa gccaaacttg tctccgacat
    31621 cgtaacgagc ccctttgaac tcacgagcaa atacacgttg tgttttattg agggtgtcga
    31681 ttgcatctgt cagctgaatt tcatttcctg cacctggagc ttgcttttcg agaatctcaa
    31741 aaatttcagg cgtgaggagg tagcgtccga taatagcaag gtcgctagga gcgtcctctg
    31801 gagctggttt ttcaacaaag gtttcaacac tgtaaagacc atcttttcct tcgccttgcg
    31861 gagcaataac cccgtaagca gatacttcgt catgagggac tggcatgaca gcgatagtag
    31921 acgcgtgggt acgctcgtag tcatccatga gttgtttggt aagtggaaca gccttttcgt
    31981 ctgtgatatc catcaagtca tcaccaagca taacgacaaa aggttcattt ccgacgaaag
    32041 ccttggcttg caaaacagca tctccgagac cgcgtggatg agtttggcgg ataaaatgca
    32101 gacgcatgtc agttgtttta tcaactagct tcaaaagatc tgttttccct ttttctttga
    32161 ggttatattc caattcgaag tttgaatcaa agtggtcctc aatagaacgt tttgatttac
    32221 cagtgacaac tagaatatct tcaatacctg atttgagagc ttcttccacg ataaactgga
    32281 tagttggttt gtctacgatt ggcaacattt ctttggcaag ggccttggtt gctggtaaaa
    32341 atcgagttcc tagtccagca gcagggatga ctgcctttct aacttttgat gtcataaaaa
    32401 tcctttctat agagggttaa gaccactcat tttctgcttt aaattcattg ttcatgatgt
    32461 cataaatggc atctttgata ttggttccgt gataaataac ttggtaaata gcctgtgtaa
    32521 tgggcatata gactccaagt tcttgggcta gttcataggc tgctcgagtc gttgaaattc
    32581 cttcgattac catgcccata ttagcttcta tatcagctag ggattctcct cgtccgagag
    32641 catctccagc tctccagtta cgagagtgga tggaagttcc cgttacgatc aaatctccca
    32701 caccagataa gccgctatag gtcaatggac tggccccgag tgctacccct aggcgggtga
    32761 tttctgctaa acctcgagcg atgatggctg ccttagcatt atcaccaaat ccaagaccat
    32821 gtaaagctcc agcaccgaca gcaataatat ttttaagagc accagcagtt tcaaccccga
    32881 taacatccgt attggtataa agtcggaagt agtgattact aaatagcttc tgaacgtatt
    32941 gagctgtttg taaatcttta gaagcagcag ttattaaagt taggtcacgc acaatggtct
    33001 cttctgcatg actaggccct gaaacaacga cgatatcact acggagatgt tcaggaattt
    33061 cttcttcaag aatggttgat aatcgtttat ggctatcagg ttctaatccc tttgatgcgt
    33121 gcatgatgat aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca agtcgtgtca
    33181 cttttgttgg gacaacaaac aaaatcgcat ccacatcttt caatgtttct gctaagtcgg
    33241 tgtaggcaat gatattttcg tctagaacga catctttaaa gtagtgctta ttagtatggt
    33301 gtgtattaat ttcattgatt tgctcgggaa gatttcccca aatacgtacc tcgtgtccat
    33361 tgtcatttaa gacttgtgaa agggcggttc cccaagaacc aggccccaag acggcgacgg
    33421 tttgtttttc catgatttcc tccttgatag agttctttct cttattctat catattctag
    33481 gggattttgc acttgcatta ctggggtatg gtggaaaaat acacaaaaac cgagacgaag
    33541 ggataaactc ttagtcttta gttaaagtga gaagaaatct gatagatagt ttgattcagt
    33601 atatgcttat aatagaggta aacaactcag gaagttcttg taggttgcga gagtttggcg
    33661 aaaaaattaa aagattacgt ttggctaaaa aaatcagtcg ttcagaattt tgtggtgatg
    33721 agtctgaatt aagtatccgt caattaatta gaattgaaaa tggagaatcc agaccaatac
    33781 taacaaagtt aaaatatatt gctgaacgtt tgggggttga agattacaag ttgatgccaa
    33841 gttatataga gttggataag gaatacctag aattgaagta tttcttgatg aggactccta
    33901 catacgaaga tgaaactatc gcccaaaaga aagagagtgt ttttgataag atttttgaag
    33961 agtattatga taggctacct gaggaagaaa gatttatcat cccaaattat tcatatctgg
    34021 cactaacgaa ctacacagtt caaaaattac cagaaaagct agttgaaata ctgtccttct
    34081 ggtgattttg cttaatttct gatgttcgag acataattga ggagatttgt cttgattttt
    34141 tcctaaaaaa gggcagactc ctcccttggt ttgatgctcg atttttaaaa taaggtgact
    34201 tattctgtta ggcatgtttt cccctgccct ctagctctat atggaacagg gagtatcaga
    34261 tttatctggc agattgtatc aaataaggct tgaaactgtt ttgaataggc gtatagactt
    34321 gagaatttga gaatatgtcg tctagcagta gagacatatt ttccggcaat atgaaggaag
    34381 agacgtcgaa aacgcttgat agtcaaggac tttacttcat caccagctag ctgctttaaa
    34441 aagaggtaga gattataagc cagacagccc atcatcatac gtacttcatt cttaatcatg
    34501 gtcgaactat ctgtcttatc cccaaagaat cctgctttcc tttctttgat aaagttttca
    34561 gcattccctc gttcacgata gaattgaaac aactcctcac tatctacact gcttaaattg
    34621 gtcaaatgaa cttttttaca accataaaaa agagtactta atcgtactct tctcttttaa
    34681 ggattagttg aattaattca aatccactag ttctcctgtt tctaggtaga ctaggcgttc
    34741 acaaatgtta gcaatgtaat cagcgaagcg ttccagatgt cctatgatat aaaggtattg
    34801 agttccattg ggaattgagg tttcttggtc tttcataagt ccaatgattt cctttgataa
    34861 ggcataataa tattggtcaa tctgttcatc tttttgagca atactaatag cttttgaggc
    34921 ttggtgcaaa ggaaaggcaa ccaataaatc ggctagcatg ctgagggata atttacccat
    34981 ttggtgtaac tgttcttcgt caggggctag ttgattttct tttagttgca aaacagcttt
    35041 ggcaatgcct gccatatggt ctcccatacg ttcaaggtct gaacaagaag acatgatgct
    35101 aatcacaaat cgaaggtcag acacttgtgg ctgctgcaag gccaacaaac gggcacaggt
    35161 caattcgata gcgctttgac cttggttgat agcatgatcc ttattgataa ttagctctgc
    35221 catctccttg tctttggagg ctaaggccag taaggctttt gaagctgttt caaggacaag
    35281 ttgccctagt cctaaaaagg attgttctaa ttcatgcaat tctaagtcaa attgatttct
    35341 cataggtttt tccttcctta tccgaaccgt cctgaaatat agtcttctgt gcgctgatct
    35401 tttggattgg taaacacgtc aacggtatct ccaaattcgc aaatttctcc tgttaagaaa
    35461 aaagcagttt tatctgaaat acgtgaagct tgttgcatgt tatgggtaac aatgataatc
    35521 gtataatcct tttttagttg ctgaatgagg tcttcaattt ttaaagtgga gatagggtct
    35581 aaggctgaag tcggctcatc cattaacaga atatcaggtt ctactgctaa agctcgcgca
    35641 atgcaaaggc gttgctgctg accgccagat aaggacatgg cactcttttt aagatcatct
    35701 ttgacttctt cccaaatggc tgcccctttt aaagatttct ccactaaggc atctaattgt
    35761 tttttgtctc gaataccatg tgtccttggg ccataagcca cgttatcata gatagacatg
    35821 gcaaagggat taggctgttg aaaaaccatc cctacacgct tacgtagctg attaaggttg
    35881 aatttgctac tataaatatc ttgctcatct aagaggactt ggccttcaat atggcaagaa
    35941 ggaaccaaat cgttcatccg gttaagggtt tttagaaaag ttgatttgcc acaaccagat
    36001 gggcctatca aggcagtaat ctgtctttct ggtaattgaa tcgaaatatt ttttaaggct
    36061 tgaaaatccc cgtaaaataa gtctaggtgt ctgactgaaa atgttcccat actaggaagc
    36121 tcctttcaca agttttcgag ataataagct tgatagagta tttatcatta aaacagtaat
    36181 aatcaaaatc acgccggtag cataggcttc attgacatgt agcccctcac ttgacagcat
    36241 atacatatgt agggctagag aacggcctga agacatgaga ctacttggcg tattggtaga
    36301 ggtacctaat gtatacatga gggcagctgt ttcaccaacg atacggccaa tagctagtat
    36361 cactccagct aaaatacctg gcatggcaac tggtagaaca attctaaaaa cagtccgtaa
    36421 cttacctgcc ccaagtccat aacttgcttg acgcatgcta tcactaacag ataaaagggc
    36481 ttcttctgtt gagcgaataa tgactggcaa caccatgata actgaggtta agattcctga
    36541 taacagagag tattgaaaac ctaagaagac tacaaagaag agcatgccaa acagaccaaa
    36601 aacaatggaa ggaatcccag ataaggtatc tgaggccaat cgcatgattt taacacaaag
    36661 ggaatctttt tttgtatatt ccacaagata aaaaccagca aaaatcccta tgggcaaggc
    36721 taaaagaaga gcaccaaaga ccagaataac ggtggaaata atcgctggca taagggaaat
    36781 gttctcagaa gtataagtcc aagaaaagag ggatagactt agatgaggta agcctttgat
    36841 gaggataaaa ccaatgatta aaaagagaga gccaaaggtt aaagctgaaa aacaataaac
    36901 gagaagtttt agcaggtatt tactcataag atgattttcc tttcaagtag gcaaagtagg
    36961 cattaatcaa gagaataagg aaaaagagaa ctgctgaggt tgcaataagg gcttccctat
    37021 gctgacctga tgcgtaagcc atttccagaa caatattggt tgttaaggtt ctggttcctg
    37081 aaaagagtcc acttggaata atcggctggt tgcctgccac caaaataact gccatggttt
    37141 cacctactgc gcgaccgatt cctaaaataa ctgctgataa aataccagat ctcgcagctg
    37201 gcaagatgac actaaaaata ctccgttcat gactagctcc tagagccaag ctaccagaat
    37261 aatacgtttt gggaactgtt cggatagcag attctgacaa actgataatg gttggcaaaa
    37321 tcattattcc taatagtaac gaagcggtta ggacactcat gccatttcct aaaaagcttc
    37381 taatccaagg caccaataat tgtaggccga aaaaaccata aacaatagat ggaatggctg
    37441 ccatcaagtt gatagctgat tttaagaagc catagacggg ctttggacaa taataaacca
    37501 taaacaccga tgtcaagatg cctgttggca ccccaatcac aatcgctcct aaggtaatta
    37561 ataaggaacc aacgatcatt ggtaaaatac catagcttgc cggaatgttc gttggcgacc
    37621 aatcactgcc taataaaaaa cgggcaaagc cgtagttagc tatgaaaggt aagccattac
    37681 taaaaataaa gaaacagatt agcaaaatag ctacaacagc tactgttgca ctcatgaaaa
    37741 aaattgccct aaaaactgct tctttgaagg cttgttttgt cacatcttgt cctttctagt
    37801 gaagaaagta agggagatac gacacctccc tacttgcctt ctttatctta ttgtacgatg
    37861 aaacgtctgc atctctttag agatttatgg agcaaacatt ttatttaatc ttgtcccagg
    37921 tggttaattt gccactaaaa acgtctgcaa gttcagccat actgacttgg cttgccttat
    37981 tgtcattatt gaccacaaca gcaataccgt ctaaagcaat agcatcatgg gtgagactct
    38041 taccttcttc aggagttaat tccctagaaa ccataccaat atcagcggtt ttctccttaa
    38101 cagcggtaat acctgctgaa gacccattag aggtaatatc aatcgtaact tctggatttt
    38161 cttttttata agcttctgct aatttttcca ttaaagaaga tactgaagtg gaacctacaa
    38221 cagacaactt gcctgataag tgttggcttg tatattccgt ggtttcggtt ttagcttcaa
    38281 taaatttatt atctgtgacc acttgttgac cttgtttgga gtggataaag ctgataaaat
    38341 cttgacctag cttggaaaga ttagaagacc aaacaatgtt gaagggacgt tgaagagggt
    38401 attcaccatc taaaactgtg tctcgactag ccttgacacc atcaatctct aaagccttga
    38461 cagatttcgt taaagatccc aaggagatgt agccgatagc attagcattc ccttgaactg
    38521 ctgagagaac accttctgta ctattttgaa tcacagctgt tttggcagtg ttgtcaattt
    38581 ttttatcacc gtcttttttg agaatccctg tgatttctgt gaaggcaccc cgtgtcccag
    38641 agccattttc tcgtgaaatc acctcaatcg ttcctgaagc tgactgtttg gaagcagctg
    38701 actgattgcc acaggcaaca agcccaaatc ctgataagcc aatggctgca agagtaagca
    38761 tttttttgaa tttcataata atcaccttta tctctatgta tttttcttgt gtaggcttac
    38821 tacatttata gtctaacaag tctttgtaaa ggtttatccc tgattcatgt aaagattgtg
    38881 taaagaatca aaaaaagcca cttttgaaaa atggctgctc ctaaaaatag ctttaaaaat
    38941 tattagtcct gtgcgaaaga ttggttagga agaaaaatcg tgaagcaact gcctctgcca
    39001 agctgactcg tcaccgtgac ttggccacct aataattgac tgagttcttt gacaatggca
    39061 aggccaagac cagtgccacc agtttgtctg cttcgacctt tattaactcg gtaaaaacgt
    39121 tcaaaaatac gatcctgctc taattgacta ataccaatcc ctgtatctga tacagaaatc
    39181 ttaatgcctt cgttcacctt ttgggtcttg acctcaattt ttcccccttg ttcagtgtaa
    39241 cggatggcat tggataaaag attgagtaag atttgggaaa gtaattgact atctgatacg
    39301 agggtgacat catctggcac ctgcaccttt agctgtaaat ccttcttctt gagctgaggt
    39361 tgcaagcttt gagtcaaatc ctgtacaaat tctgccaaag aaagggtcgt ccattgtata
    39421 ggcatttgtt gagccttaga taaggtaaga agatgctcaa caatatgctc aagacgcaaa
    39481 ctttctttgt aaataatgtc tagaaagtca tccttgagcg cttcttcttc agctgacatc
    39541 cccttaatgg tttcagcaaa gcccttaatc gaagtaactg gtgtcctcaa ttcatgggag
    39601 gcatttgaga caaaggctaa atttaacttt tcataagttc taatcgttgt taaatcatat
    39661 agcaagacga gcacagcttc cacagattgg gtggggctaa aaacgggaac tgctgtcact
    39721 tctaaaatca agtcaccctc atgaaaccca cttacttctt gttttaacct tgttttttga
    39781 tcaaaggctt ggtgaactaa attccgaata tccatccgtt tgaggtcatc aagtgaactt
    39841 atgtcgccgt ccacatcggg aaaataatga ggcagagagc gactggataa taacatctga
    39901 ccttgagcgg aaactaaaaa cgtccccatg gttaggtgcg acagaagaac ctccattgtt
    39961 tcggctagat ccttgtattg ctgatcctgt tgggagactt tggtttttag gccagacaca
    40021 tactgagcca aagactttaa gtcttcttgc cctttttcta aaaagtattc actactggtc
    40081 aagagaggtt ggtgcaaggt ctcaaaagca acttcccatt tccaaaggca aaagagccag
    40141 tagccaccta gtcccaaaga aagggctaga agaaagagac cgatgccttt actgatccaa
    40201 gttaatgcca tccctgcaat cagaatgagg ctaacactta gattgactag ccaaaattga
    40261 aggtagcgtt tcatctataa ctccttgaac ttataaccat aaccccgaat ggttcgaata
    40321 aattgagggg ctttaggatt gtcttcaatt ttttccctca acttaccaat atgaacgtcc
    40381 accaaacgtg tttcctgccc aaagtcatac ccccagatac gttccaaaag acgctctcta
    40441 gtcagtgtca tgttgggatg tttcataaga tagagcaaga gttcaaattc ttttggggtc
    40501 aaactcagta acttattcgc cttgtagact tcatgacgct cagggtatac tttcaaggtc
    40561 ccaaatagcc aagaatcgtc agcgatatta tctgaatcat ctccttcttg ttctccttta
    40621 gttcgcctga ggacagcctt gacacgcgcc agcaattctc tagggctaaa aggcttggtc
    40681 aggtagtcat cagcccctaa ttccaaggcc aaaaccttat caaattcatc acttttcgca
    40741 gaaaccatca taattggagt tttgacgcct ttggctctca gccgcttaca aacttccatg
    40801 ccatctaatt gtggtaacat gatatcaagc aagataaaat caaagggttc tgtttctgcc
    40861 aaagctaagg ccttccgtcc atttgtcacc aattgagtag aaaagccttc cttacttaaa
    40921 tggtagtcaa gcaatttcag aatgtgttct tcatcatcca ctaataagac ttgttttgtc
    40981 atctattatc tcctattggt aacattataa cacaattatc agaaatccta acattgctaa
    41041 atcagattaa atttgcctat caagactagt atctggtcaa acgctcaatc atctccttgt
    41101 gctctggata ggtcgccagt agatctaccc tttcaaataa ttcaaaatcc tcaaattcaa
    41161 aaccaggagc aacaagacaa gaaaccagag catcatcctt atcaactgtt gatccccaaa
    41221 tagtgccctt aggaacacag tagtgaagtt gttgcccttt ggatatgtcc aggcctaaag
    41281 tgactgcttc gtagtgacca tctgctgtaa tcatgtgaac agtaagtggg gatcctgcat
    41341 gaaaatacca gatttcatct gctgtcaatc ggtgaaaatg tgaaggattc gtttcttcta
    41401 ataagaaata aatactggta taaagcgccc ttcccttacc agcaaggttt atagtgtctg
    41461 aagctttttt tgtttgtcta aaatagccac cttcaatatg gggagctaac tctagagttc
    41521 ttatcaagtc ttctttatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg
    41581 ttttacgatt tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga
    41641 agtacaaatc aatcgcccta aaaaaagaat tagcgaatga ttctggtaaa aaaaatgcca
    41701 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcgc
    41761 tgtgaactat tgtcatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc
    41821 tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca
    41881 aactccacgt aaatccagca aactcaagcc actaacagtt gaagataaag cctataacca
    41941 tgcgctatcc aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa
    42001 aatgatttca acaacctatc gaaatcatcg taaacacttc ggattacgaa tgaatttgat
    42061 tgctggcatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt
    42121 aggtgaatta gtgaagcgtt taggcaagtg tctctggtta cgacgtcatg gactctaaat
    42181 cgattatatt taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta
    42241 gcgtctaaca attaggaact ttagttccaa taactttaag attacgacgt tttaggacat
    42301 aaatcgatca tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat
    42361 ttggcgttag ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc
    42421 tggcgcttct actccaagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct
    42481 gagggctaga cggctgtcgc gttctgggct ttcatccaag atacgtgtat gtgcatagta
    42541 tttgttaaag gattgagcca ggctaattgc aaatttagca atgatagaag gttcaaagtt
    42601 atctgccgca cggttgataa tacgtgggaa gtcttgaatg agtttaatga tttcccagct
    42661 ttcagtatca ttcaagctat agttgccagc tgtttctggt ttgaaatcgg ctttgcgtaa
    42721 gatagattgg atacgagcgt aggcatattg aacgtaaggt ccagtttcac cctcgaagga
    42781 taccatagcc tctaggtcga agtcgtatcc atttgtacgg tcggttttga ggtcatagaa
    42841 tttaatggct ccaatcccaa cagcatgtgc tacttggtct ttgttttcta gttcaggatt
    42901 tttagcctcg atttggacct tggcacggct aacagcctct gcaacagtag gctctagcaa
    42961 gatgacattc cctttacgag tagagagttt cttcccttct tttgtaacca aaccaaaagg
    43021 aacgtgagta atgtcgtcac tccagtcgta gcccatctct tgcaagacag ctttgagctg
    43081 tttaaagtgg gcagattgtt cttgaccaac gacatagata gatttagcaa attggtattc
    43141 gtttttacgg tagagggctg cagccaagtc acgtgtgata tagagagttg caccatcaga
    43201 cttcttgatg agggctggat gttcaattcc atatttctca agattcacaa cttgggcacc
    43261 ttctgattca agaagtagtc ctttttcaga aagaatgtct acaactgcat ccatcttatc
    43321 attgtagaag gcttctccgt tatagctgtc aaattcaacc ttcaattcat tgtaaaggcg
    43381 gttaaattcc actaaacttt catcgcggaa ccattgccaa agagcgagag cttcctcatc
    43441 tccattttca agtttacgga accattcgcg cgcttcttca tccaagctag ggtcattttc
    43501 agcttcagcg ttgatgcgga catagagttt aaggagttca tcgattggat gagcttttac
    43561 agcttcttcg tcgccccatt ttttgtaggc aacaatcaac atcccaaatt gtttacccca
    43621 gtctcccaaa tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa
    43681 gctatctccg ataacagttg aacgcaggtg gccaatagaa aatggtttag cgatattcgg
    43741 actagacatg tcgataacaa cattttcttg tttaccaata ttttggtcag catagtgttc
    43801 tttttcagtg gtaacagctt gcaatacttg agcagaaatg gcagatttat caaggaaaaa
    43861 gttaacgtaa ggtcctgttg cgacaacttt ttcaaaggct tggctgttca ttttttcagc
    43921 cagttcagcc gcaatcattt gtggtgcttt acgttcgact tttgcaagag aaaaagcagg
    43981 gaaagcaatg tctcccattt ctgagttttt aggggtttcc agtaacttta aaatagcctc
    44041 ttggtccagg ctatcaatga tgctagataa ttcgctagca atcaattctt ttgtattcat
    44101 taagagctcc tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa
    44161 atttttgaaa tctcctgttt ttttggtata atatggttat aaatatagtt ataaatatgc
    44221 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
    44281 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
    44341 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
    44401 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
    44461 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
    44521 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
    44581 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
    44641 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
    44701 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagtta tcacccggca
    44761 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
    44821 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
    44881 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
    44941 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
    45001 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
    45061 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
    45121 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
    45181 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
    45241 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
    45301 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
    45361 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
    45421 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
    45481 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
    45541 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
    45601 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
    45661 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
    45721 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggcaaa accaatccaa
    45781 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
    45841 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
    45901 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
    45961 gaattatccg gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
    46021 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
    46081 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactag
    46141 gaaatgtgcc agctcacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
    46201 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
    46261 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
    46321 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
    46381 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
    46441 ggcgccatgc tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc
    46501 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
    46561 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
    46621 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
    46681 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
    46741 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
    46801 atgacgggat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
    46861 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
    46921 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
    46981 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
    47041 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
    47101 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
    47161 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
    47221 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
    47281 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
    47341 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
    47401 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc
    47461 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
    47521 agtcttaggc gccctcctta ctgggaaata tgaagctatt tatagtatcg gggcttggtt
    47581 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
    47641 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
    47701 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
    47761 tatcaaccag attcagaacg ttgtcatgat gaccttccaa attcttttca gacttcccct
    47821 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
    47881 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
    47941 tcgttttgcc aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
    48001 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
    48061 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
    48121 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
    48181 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
    48241 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
    48301 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
    48361 ggatatccca gatgaagagt tggttggaag tcttagcttt gaaaatgtga cctttaccta
    48421 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
    48481 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
    48541 tctctttgat ccacaggacg gggccattaa aatcggtggc aaggatattc gagaagtgag
    48601 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
    48661 aacgattgca gataacttga gacaggggaa ggggaatgct actctatttg aaatggagcg
    48721 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
    48781 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
    48841 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
    48901 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
    48961 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
    49021 tcaaggacga ttgattggtc aaggtacgca tgcagacttg gttgccaaca atgccgttta
    49081 ccgtgaaatc tatgaaacac agaaat
//
LOCUS       DBDESG010000007        48947 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.007, whole genome shotgun sequence.
ACCESSION   DBDESG010000007 DBDESG010000000
VERSION     DBDESG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 48947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.007"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            5..75
                     /locus_tag="ACKB38_000379"
     tRNA            5..75
                     /locus_tag="ACKB38_000379"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="ACKB38_000380"
     tRNA            110..183
                     /locus_tag="ACKB38_000380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="ACKB38_000381"
     tRNA            266..355
                     /locus_tag="ACKB38_000381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="ACKB38_000382"
     tRNA            366..439
                     /locus_tag="ACKB38_000382"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="ACKB38_000383"
     tRNA            443..515
                     /locus_tag="ACKB38_000383"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="ACKB38_000384"
     tRNA            528..608
                     /locus_tag="ACKB38_000384"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="ACKB38_000385"
     tRNA            613..683
                     /locus_tag="ACKB38_000385"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="ACKB38_000386"
     tRNA            698..770
                     /locus_tag="ACKB38_000386"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="ACKB38_000387"
     tRNA            781..852
                     /locus_tag="ACKB38_000387"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="ACKB38_000388"
     tRNA            862..945
                     /locus_tag="ACKB38_000388"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="ACKB38_000389"
     CDS             1052..2329
                     /locus_tag="ACKB38_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGL7979218.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /gene="thrC"
                     /locus_tag="ACKB38_000390"
     CDS             2405..3889
                     /gene="thrC"
                     /locus_tag="ACKB38_000390"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGL7979219.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="ACKB38_000391"
     CDS             3950..5230
                     /locus_tag="ACKB38_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGL7979220.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5234..5854
                     /locus_tag="ACKB38_000392"
     CDS             5234..5854
                     /locus_tag="ACKB38_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGL7979221.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7132
                     /locus_tag="ACKB38_000393"
     CDS             6020..7132
                     /locus_tag="ACKB38_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGL7979222.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7455..7880
                     /locus_tag="ACKB38_000394"
     CDS             7455..7880
                     /locus_tag="ACKB38_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGL7979223.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7891..8250
                     /locus_tag="ACKB38_000395"
     CDS             7891..8250
                     /locus_tag="ACKB38_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGL7979224.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8357..9001
                     /gene="pcp"
                     /locus_tag="ACKB38_000396"
     CDS             8357..9001
                     /gene="pcp"
                     /locus_tag="ACKB38_000396"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGL7979225.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9029..9886)
                     /locus_tag="ACKB38_000397"
     CDS             complement(9029..9886)
                     /locus_tag="ACKB38_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGL7979226.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10016..11158
                     /gene="tgt"
                     /locus_tag="ACKB38_000398"
     CDS             10016..11158
                     /gene="tgt"
                     /locus_tag="ACKB38_000398"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGL7979227.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11256..13073
                     /locus_tag="ACKB38_000399"
     CDS             11256..13073
                     /locus_tag="ACKB38_000399"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGL7979228.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQESKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13226..14377
                     /gene="nagA"
                     /locus_tag="ACKB38_000400"
     CDS             13226..14377
                     /gene="nagA"
                     /locus_tag="ACKB38_000400"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGL7979229.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAIDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14540..15598
                     /locus_tag="ACKB38_000401"
     CDS             14540..15598
                     /locus_tag="ACKB38_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGL7979230.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15749..16114
                     /locus_tag="ACKB38_000402"
     CDS             15749..16114
                     /locus_tag="ACKB38_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGL7979231.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16190..17131
                     /gene="comGA"
                     /locus_tag="ACKB38_000403"
     CDS             16190..17131
                     /gene="comGA"
                     /locus_tag="ACKB38_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGL7979232.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDHKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17079..18095
                     /gene="comGB"
                     /locus_tag="ACKB38_000404"
     CDS             17079..18095
                     /gene="comGB"
                     /locus_tag="ACKB38_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGL7979233.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEVGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18097..18423
                     /gene="comGC"
                     /locus_tag="ACKB38_000405"
     CDS             18097..18423
                     /gene="comGC"
                     /locus_tag="ACKB38_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HGL7979234.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQAKAYKEYNDKNGGA
                     NRKVND"
     gene            18416..18820
                     /gene="comGD"
                     /locus_tag="ACKB38_000406"
     CDS             18416..18820
                     /gene="comGD"
                     /locus_tag="ACKB38_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGL7979235.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18783..19085
                     /gene="comGE"
                     /locus_tag="ACKB38_000407"
     CDS             18783..19085
                     /gene="comGE"
                     /locus_tag="ACKB38_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGL7979236.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19048..19509
                     /gene="comGF"
                     /locus_tag="ACKB38_000408"
     CDS             19048..19509
                     /gene="comGF"
                     /locus_tag="ACKB38_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGL7979237.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            19487..19900
                     /gene="comGG"
                     /locus_tag="ACKB38_000409"
     CDS             19487..19900
                     /gene="comGG"
                     /locus_tag="ACKB38_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGL7979238.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20037..20631
                     /locus_tag="ACKB38_000410"
                     /pseudo
     CDS             20037..20631
                     /locus_tag="ACKB38_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20692..21645
                     /locus_tag="ACKB38_000411"
     CDS             20692..21645
                     /locus_tag="ACKB38_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGL7979239.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21696..22886
                     /locus_tag="ACKB38_000412"
     CDS             21696..22886
                     /locus_tag="ACKB38_000412"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGL7979240.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22887..23018
                     /locus_tag="ACKB38_000413"
     CDS             22887..23018
                     /locus_tag="ACKB38_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979241.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            23035..23406
                     /gene="rnpA"
                     /locus_tag="ACKB38_000414"
     CDS             23035..23406
                     /gene="rnpA"
                     /locus_tag="ACKB38_000414"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGL7979242.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNRFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23381..24205
                     /locus_tag="ACKB38_000415"
     CDS             23381..24205
                     /locus_tag="ACKB38_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGL7979243.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24224..25210
                     /gene="jag"
                     /locus_tag="ACKB38_000416"
     CDS             24224..25210
                     /gene="jag"
                     /locus_tag="ACKB38_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGL7979244.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25261..25884
                     /locus_tag="ACKB38_000417"
     CDS             25261..25884
                     /locus_tag="ACKB38_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGL7979245.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26015..27472
                     /locus_tag="ACKB38_000418"
     CDS             26015..27472
                     /locus_tag="ACKB38_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGL7979246.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27495..27776
                     /locus_tag="ACKB38_000419"
     CDS             27495..27776
                     /locus_tag="ACKB38_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979247.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27853..28338
                     /locus_tag="ACKB38_000420"
     CDS             27853..28338
                     /locus_tag="ACKB38_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7979248.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28355..29020
                     /locus_tag="ACKB38_000421"
     CDS             28355..29020
                     /locus_tag="ACKB38_000421"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGL7979249.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGIDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            29024..29887
                     /locus_tag="ACKB38_000422"
     CDS             29024..29887
                     /locus_tag="ACKB38_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGL7979250.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29889..30593
                     /locus_tag="ACKB38_000423"
     CDS             29889..30593
                     /locus_tag="ACKB38_000423"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGL7979251.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKTWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30772..32445
                     /locus_tag="ACKB38_000424"
     CDS             30772..32445
                     /locus_tag="ACKB38_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGL7979252.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYNLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32557..33648
                     /gene="ulaG"
                     /locus_tag="ACKB38_000425"
     CDS             32557..33648
                     /gene="ulaG"
                     /locus_tag="ACKB38_000425"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGL7979253.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKIRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33762..35738
                     /gene="tkt"
                     /locus_tag="ACKB38_000426"
     CDS             33762..35738
                     /gene="tkt"
                     /locus_tag="ACKB38_000426"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGL7979254.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35855..36154
                     /gene="yajC"
                     /locus_tag="ACKB38_000427"
     CDS             35855..36154
                     /gene="yajC"
                     /locus_tag="ACKB38_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGL7979255.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36200..36628
                     /locus_tag="ACKB38_000428"
     CDS             36200..36628
                     /locus_tag="ACKB38_000428"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGL7979256.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36630..37040
                     /locus_tag="ACKB38_000429"
     CDS             36630..37040
                     /locus_tag="ACKB38_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGL7979257.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37340..39991
                     /gene="adhE"
                     /locus_tag="ACKB38_000430"
                     /gene_synonym="adhC"
     CDS             37340..39991
                     /gene="adhE"
                     /locus_tag="ACKB38_000430"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGL7979258.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(40977..41285)
                     /locus_tag="ACKB38_000431"
     CDS             complement(40977..41285)
                     /locus_tag="ACKB38_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGL7979259.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(41282..41590)
                     /locus_tag="ACKB38_000432"
     CDS             complement(41282..41590)
                     /locus_tag="ACKB38_000432"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979260.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(41595..42890)
                     /locus_tag="ACKB38_000433"
     CDS             complement(41595..42890)
                     /locus_tag="ACKB38_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGL7979261.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTEFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(42908..44317)
                     /locus_tag="ACKB38_000434"
     CDS             complement(42908..44317)
                     /locus_tag="ACKB38_000434"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGL7979262.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            44475..45203
                     /locus_tag="ACKB38_000435"
     CDS             44475..45203
                     /locus_tag="ACKB38_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGL7979263.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            45662..45856
                     /locus_tag="ACKB38_000436"
     CDS             45662..45856
                     /locus_tag="ACKB38_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGL7979264.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(45978..46144)
                     /locus_tag="ACKB38_000437"
                     /pseudo
     CDS             complement(45978..46144)
                     /locus_tag="ACKB38_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(46222..47532)
                     /locus_tag="ACKB38_000438"
     CDS             complement(46222..47532)
                     /locus_tag="ACKB38_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGL7979265.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            47769..48641
                     /gene="nadC"
                     /locus_tag="ACKB38_000439"
     CDS             47769..48641
                     /gene="nadC"
                     /locus_tag="ACKB38_000439"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGL7979266.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            48772..>48947
                     /locus_tag="ACKB38_000440"
     CDS             48772..>48947
                     /locus_tag="ACKB38_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGL7979267.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGIKPR"
BASE COUNT        14948 a         8734 c        10707 g        14558 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtaggggag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttatgaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaaac
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgtccgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagatt taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggata cttatccaaa
     2521 ggtagatttg aactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagt tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcga atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaagga tggtgtcagc aagattcaag agttacaaat
     2941 gaccactcag actggcgaca atactcatgt tattgctatt gatggtaact ttgacgatgc
     3001 gcaaacaaat gtgaagcaca tgtttaacga cgtggctctt cgtgaaaaat tgactaccaa
     3061 caagttgcaa ttttcatcag ctaactctat gaacattggt cgtctggtgc cacaaattgt
     3121 ttattatgtt tatgcttacg ctcaattggt taagactggt gaaattgtag ctggtgaaaa
     3181 ggttaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaattggt ttgccagttg gtaaattaat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttt aaaacacgtg tctatgacaa aaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttgggaaa
     3421 taatgctgaa aagacaactg aacttatgaa tgccttgaac acgcaaggac aatataagtt
     3481 gacagacttt gatgcagaga ttttggacct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacggcagca gagatcaagc gtgtttgtga gttagattct tatatcgagg accctcatac
     3601 agctgttgct tcagcagttt ataaaaaata ccaatcggcc actggagatg taactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga aatagctcca attcgtcaca agacaacagt
     3841 ggcagctgct gacatgcaag cagcggttga ggcttattta ggactttaag acagagggag
     3901 caaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 aggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttgtgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagttgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttgggacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggaatg tggtgtatcc gcattgggac
     5101 aggatatctg atggggattg tgcttggttg gggcttgcct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgagctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagc atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg tacagtatgg
     6181 tgatactttg agcaccattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg accgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccaattgc agaagttaca aagacagtga ttgcttctga
     6481 agaagtggca ccatctacgg gcacttctgt cccagaggag caaacgaccg aaacaactcg
     6541 cccagttgaa gaagcaactc ctcaggaaac gactccagct gagaagcagg aaacacaagc
     6601 aagccctcaa gctgcatcag cagtggaagt aactacaaca agttcagaag caaaagaagt
     6661 agcatcatca aatggagcta cagcagcagt ttctacttat caaccagaag agacgaaaat
     6721 aatttcaaca acttacgagg ctccagctgc gcccgattat gctggacttg cagtagcaaa
     6781 atctgaaaat gcaggtcttc aaccacaaac agctgccttt aaagaagaaa ttgctaactt
     6841 gtttggcatt acatccttta gtggttatcg tccaggagac agtggagatc acggaaaagg
     6901 tttggctatc gactttatgg taccagaacg ttcagaatta ggggataaga ttgcggaata
     6961 tgctattcaa aatatggcca gccgtggcat tagttacatc atctggaaac aacgtttcta
     7021 tgctccattc gatagcaaat atgggccagc taacacttgg aacccaatgc cagaccgtgg
     7081 tagtgtgaca gaaaatcact atgatcacgt tcacgtttca atgaatggat aaacccgact
     7141 tgataacatc attttgacga atgagatcta gctttcgtga tggaaagcga ttctcgttcg
     7201 ttttttcttt gtcatactct ttgaaaatct cttcaaacca cgtcagtttt atctgaaact
     7261 tcaaagctgt gctttgagca acctgcgact agcttcctag tttgcttttt gattttcatt
     7321 gagtatcaat ttgaatggaa aatggaaagt tatcatcttg taatgagtta agcaacattc
     7381 ttgcaatcta ttttacttta tatcacaatt aattagtcaa atattgataa atcaataaaa
     7441 agagagggga agaaatgcta gagattcaag atttactgta tcaactccgc ttgtctgagc
     7501 aagcgagtac gcaattgttt gaaaaaaggc ttgggattag tttgacacgg tatcagattt
     7561 tactgttttt gctggagcat tctccttgta accaaatggc ggttcaggag cgtttgaaaa
     7621 ttgatcaggc tgctttgaca cggcatttca aaattttgga aacggaaggt ttggtggagc
     7681 gtcatcgtaa tcctgaaaat cagcgggaag tgttggtaga ggctgcgaag tatgccaagg
     7741 agcagttagt ggtgaatccc cctctgcaac atatcagggt taaggaagag atagaaagta
     7801 tcttaacaga gtttgagaga acagaactca gccgtttatt aaataaattg gttttgggta
     7861 ttgaaaatat agaaatttaa ggagaaatag atgtcaatta ttttaacaac gatcgttgct
     7921 ttggagcatt tttacatttt ttatttggaa agtattgcca cgcaatcaga tgcgactagt
     7981 cgtgtattta atatggaaaa ggaagaattg gctcatccgt cagtaagttc attgttcaaa
     8041 aatcaaggaa tttataaggc tctgctagga gtctttctct tgtatgtcat ttatttctca
     8101 cagaatttag aaattgtgac tatttttgtc ttatttgtga ttggtgctgc gacttacggc
     8161 tctttaacag cggataaaaa aattattttg aaacaaggtg gatcagctat tttggccttg
     8221 attagtattt tactctttaa atacacttga aggtcgattc taatctcgct aatccttttt
     8281 aatccagaat aagggaaata tgttatactt gtttttaaga aaaaagtctc attgaattgg
     8341 ttttgaggag ttagaaatga aagtattagt gacaggtttt gagccctttg gaggggaaaa
     8401 gggcaatcca gctttggagg ccattaaagg tttaccagct gaaatccatg gtgctgaggt
     8461 ccgttggcta gaggtgccga cagtttttca caaatctgct caagtattgg aagaagagat
     8521 gaatcgttat caacctgact ttgtcctttg tattgggcaa gctggtggaa gaactagttt
     8581 gacacctgaa cgagtgacca ttaatcaaga cgatgcatgc atttctgata acgaagataa
     8641 tcaaccgatt gaccgtccca ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc
     8701 gattaaagcg atggttcaag ctataaaaaa agagggctta ccggcctctg tttccaatac
     8761 ggcagggact tttgtctgca gccatttgat gtatcaggct ctctatttgg tagaaaagaa
     8821 atctccatat gttaaggcag gttttatgca tattccttat atgatggaac aggtggtgaa
     8881 cagaccgact actccagcta tgagtttagt ggatattcgg cgagggatag aagcagcaat
     8941 cggcgctata atagaacatg gagatcagga actcaagttg gtaggcggag aaactcattg
     9001 atagaaaaaa gcttgagggg aaaaaccttc aagcttttgg acgttttcgg gccaatactg
     9061 ctcggtaaaa cataatttta gtgcattgga tataaggtag gagtgaaaaa ctagcaatgc
     9121 caaaggtaat ccaattgagg aagtaccaag gaagaagctg taaatctagg acaaagtgct
     9181 ggaacttgta gcccttcata aaggaacggc tagtttttag gattcgtctt ggtgggacct
     9241 gtcctaggtc tagactataa cagagaagaa attccacctg tgaataggca taatactgtg
     9301 gaatatagag gatatttcct acaatgatca agatgagact tgcaagaaag tagagtccaa
     9361 agaccatgag gaaacgctcg gtttcaactg atgagagatc tagatttgga aactcaggat
     9421 gtagggtgac gaattttttg gctaaaaagc tactataaaa gaggaggtaa atcccaagta
     9481 aattagggat actccataaa aagagataga aacgtttgag aagtagggtc aaaaaggttt
     9541 gagaaaagcg ctcctcatca aagagagcta ggctgttttt tacagatggc tccgttttag
     9601 aatctttcat gagtgtcagt gttgcataga cggaactggt caaaagaata gtcccgataa
     9661 aggagactag tagaggaaag aggtaggttt gaagtatttg gccaagtatg ctgaaaaatg
     9721 gctgttctaa aacagtcccg tggatccgag ataagggatt aagaaaacca gataagatga
     9781 ccagcatact gggaaggata tagaggagaa agagacgggg ggtgtcagcc tgaaaatgtt
     9841 ttgactcctg acgaattgtt tttaaatcaa tttttggata gttcattctc ttattatacc
     9901 atagttctta tacatagttc gtgacagttc ctactttttt tgataaaatc atatagtgtg
     9961 tccttgggca cactgtatga actgggactg tctttcccag cttcggaggt aaaaaatgtc
    10021 agattcacca atcaaatatc gtttgattaa gaaagaaaaa cacacaggag ctcgtctggg
    10081 agaaatcatc actccccacg gtacctttcc gacacctatg tttatgccag ttgggacaca
    10141 agccactgtc aaaactcagt cacctgaaga attgaaggag atgggttcgg gaattatcct
    10201 atcaaacacc tatcatctct ggcttcgccc tggagatgaa ctcattgcac gcgctggtgg
    10261 tctccacaag ttcatgaatt gggaccagcc tatcttgaca gatagtggtg gttttcaggt
    10321 ttattcttta gcagatagcc gtaatatcac agaagaagga gtaaccttta aaaatcatct
    10381 aaatggttct aagatgttcc tatccccaga aaaagccatc tctattcaga ataatctggg
    10441 ttcagacatc atgatgtcct ttgatgaatg tcctcagttt tatcaacctt atgactacgt
    10501 taagaaatcg atcgagcgta ccagccgttg ggctgagcgt ggtttgaagg ctcaccgtcg
    10561 tccacatgac caaggtttgt ttggaattgt gcaaggtgca ggatttgaag accttcgccg
    10621 ccaatcagct catgatcttg tcagcatgga tttctcaggc tactctatcg gtggtttggc
    10681 agtgggagaa acccatgaag agatgaatgc ggtcttggac tttacaactc aactgctgcc
    10741 tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca gatagcttga tcgatggggt
    10801 cattcgtggg gtggatatgt ttgactgtgt cttaccgact cgaattgctc gtaacgggac
    10861 ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc cagtttgctg aggactttac
    10921 gccactggat cctgagtgtg attgctacac atgtaataac tatacacgcg cttaccttcg
    10981 tcacctgctc aaggctgatg aaacctttgg tatccgcttg actagctacc acaatcttta
    11041 cttcttgctt aacctgatga agcaagtgcg acaagccatc atggatgaca atctcttgga
    11101 attccgtgag tattttgtgg aaaaatatgg ctataataag tcaggacgta atttctaaaa
    11161 tggaattgat ataaaaaaat cctaagtttt ctcttaggat ttttcttctt tttttgatag
    11221 aataaagtgt acaatgaaag gaagaataaa ctcgtatgcg cattaaatgg ttttccttga
    11281 ttaggattat aggtttactt ttggtactct tgtatcactt ctttcagacg atctttcctg
    11341 gaggattttt cggggtagat gtctttttca cattttcagg tttcctgatt acagctctac
    11401 tcattgaaga attttctaag aacaatgaga ttgatttgat aggatttttt agaagacgct
    11461 tttatcggat tgtgccacct gtggttttga tggtcttggt gaccatgcct tttactttct
    11521 tggttcgcca agactatgtt gctggaattg gtggccagat tgcgggcgtc ttaggcttta
    11581 tgaccaactt ctatgaactc ctaacaggtg ggagttatga atctcagttc attcctcatt
    11641 tgtttgttca taattggagt ttggcagttg aggttcacta ctatattctt tggggattgg
    11701 cagtttggtt cttatccaaa caagctaaat caaatggtca gttgaagggg atggtctttc
    11761 tcttatctgc tgttgccttc ttgatcagtt tcttctccat gtttattggt agttttctag
    11821 tgacctctta ttcctctgtt tatttctcca gtttaactca tgtctatcca ttctttttag
    11881 gaagtatgtt agcaactatt gtaggcgttc gtcagacgac ttccctcgtc aagcagttgg
    11941 ataaaatctg ggatttacga aagactttgg tagtttttgg aggaggcttt ggtttcctag
    12001 ttcttttgac tttctttgtc aaattcactt atctttttgc ctatcttatc ggcttcttac
    12061 ttgccagtct tgcagctctt gccatgattc tggcggcgcg tgtcttacat gaaaagacac
    12121 atcatataca ggagtcgaag attatcagct ttttagcgga tactagctat gcggtttatc
    12181 ttttccattg gcctttctat atcattttct cacagttgac atcaaatctt cttgctgtat
    12241 tactgactct gatttgttct tatggctttg ccagtctgtc attttatgta ttggaacctt
    12301 ggattgcagg caagaacaca cctattgtcc aaacccttcg tcccctgcct tatattcacg
    12361 caattcttgc agcaggtaca ggaatcttga ccatcattgt ctgcacggtg accttgttgg
    12421 caccacaagt gggagcgttt gagacagact tgactgtcaa tggcttgaag caagctgcaa
    12481 caaatattgg ccagaccaag gtgatggcag aacgggcaga tgcaaacagt ttgggaattg
    12541 ctgatggcac tatgttaatt ggtgactcag tggctttaag ggcaaataca gcactacaga
    12601 cagctcttcc tggagcacag attaacgcgc aggtcagcgt aacaaccaag accgcaaatg
    12661 aaatcatgct aaataatagc cagaataaat ttttacctaa gacggtggtc attgcgactg
    12721 gggtaaataa tcctgagaat tacaaggatg actgggacag tatcgtgaaa aatcttccta
    12781 agggacacca tatgattttg gtgactcctt atgagggaga taagacaaaa gagacctatg
    12841 ccatcgttga gaaggctgct gcctatatga gagaattggc agagaagaca ccttacatta
    12901 cgatagcaga ttggaatcaa gttgcgaaag agcatccaga aatttgggct ggaacagacc
    12961 aggttcattt cgggagtgag agtagcacta tcgaagcagg agcaaaattg tatgcagata
    13021 cgattgccac agctttgcag acagctcaag acaagccggt taaatcaaaa taacttgtat
    13081 taaaaaagag aagagttgga gaaatccagc tcttttaaaa tatctccaaa aaataggtct
    13141 ataccattta caaataaaaa agaaaggttt ataatgtaat tgacataata aattgtagaa
    13201 tcaatctttt aaggaggtta acattatgcc taactatatt aaagcggatc agtttttcta
    13261 cccacacgga gttcgtcgag gtggttactt ggaacttgtg gacggcaagt ttgggaaaca
    13321 tgtagagcag attcctgaag gggctgaggt gattgactat acaggttata gcattgcccc
    13381 aggtcttgtg gatactcata ttcatggata tgcaggtgta gatgtgatgg acaacaacat
    13441 tgaaggtaca ttgcatacta tgagtgaagg acttcttagt accggtgtta ccagtttctt
    13501 acccacaact ttaacagcca cttatgagca attgcttgca gtcactgaaa atcttggaaa
    13561 ccattataaa gaagcaacag gtgctaagat tcgtgggatt tattatgaag gtccatattt
    13621 cacagaaact tttaaggggg cacaaaatcc aacttatatg agagacccgg gtgttgagga
    13681 gtttcattct tggcaaaaag cggcaaatgg cttgcttaat aaaattgccc ttgcaccaga
    13741 acgtgatggg gtggaagact ttgttcgtac agttacgggc gaaggtgtga cggttgctct
    13801 tggacattca aacgcgactt ttgatgaagc caaaaaagca atcgatgctg gagcgagtgt
    13861 ttgggtgcat gcctacaatg gaatgcgtgg gttgactcac cgtgaattgg ggatggttgg
    13921 agccatgtac caattgccac atacctatgc agagttgatc tgtgatggtc accacgtaga
    13981 tccaaaggct tgcgaaattc ttatcaaaca aaaaggaaca gaaaacatcg ctcttatcac
    14041 agactgtatg acagctgggg gattggaaga cggagattat atgttgggag aattcccagt
    14101 tgtcgttgca aatggaactg cacgcctcaa atcgacaggt aacttggcag gttctatcct
    14161 caaactcaaa gatggtttga agaatgtggt cgaatgggga attgcgaatc cgcatgaagc
    14221 agtcatgatg gccagcttca acccagctaa atccgttcac atcgatgacg tctgtggcca
    14281 aatccgtgaa ggctacgacg ctgacttcat cgtattagat aaagatttgg aattggtagc
    14341 aacctaccta gatggcgtaa aacgttatca agcataagag agaaagcaga agttagagac
    14401 taacttctgc ttttttatgc agggtacttt actggtaaat aaaaagttaa aaaaatcaca
    14461 aaaaaagctt gaagaaacaa agaaaaaggc ttacaattga actataaata ttacaaataa
    14521 aaaaacggag gagtgcttta tgaaagccta tacttatgtt aaaccaggac ttgcttcttt
    14581 tgttgatgta gacaaaccag ttattcgcaa gccaacagac gctattgtgc gtattgtaaa
    14641 aaccactatt tgtggaacag acctccatat tatcaaaggg gatgttccta cttgccaaag
    14701 tggtaccatt cttggccacg aagggattgg gattgttgaa gaagttgggg aaggagtttc
    14761 caacttcaaa aaaggtgaca aggtcttgat ttcttgcgtc tgtgcctgtg gtaaatgcta
    14821 ctactgtaaa aaaggaattt atgctcactg tgaagacgaa gggggctgga ttttcggtca
    14881 cttgattgat ggtatgcagg ctgaatatct acgtgtccct catgcagata atactcttta
    14941 ccatactcca gaagacttgt cagatgaagc tttggttatg ctgtcagaca ttctgcctac
    15001 tggatatgaa attggtgtct taaaagggaa agtagaacct ggttgcagcg tagccattat
    15061 tggttcaggt ccagttggat tggctgctct tttaacagcc caattctatt caccagctaa
    15121 attgattatg gtagacctag acgataaccg cttggaaact gccctatcat tcggtgcgac
    15181 tcataaggtt aattcttcag accctgaaaa agccattaaa gaaatttatg atttgacaga
    15241 tggtcgtggt gtggatgtcg ctatcgaagc tgttggtatt cctgcaacat ttgatttctg
    15301 tcaaaagatt atcggtgtag acggaacggt tgccaactgt ggtgtgcatg gtaaaccagt
    15361 tgaattcgat ttagataaac tttggattcg caacatcaat gtaacaactg gtttggtatc
    15421 tacaaatacg actccacaat tgttgaaagc acttgaaagt cataagattg aaccggaaaa
    15481 attggtaact cactatttca aactcagtga aattgaaaaa gcctacgaag tcttcagtaa
    15541 ggcagcagac caccatgcca ttaaggtcat tatcgaaaac gatatctcag aagcctaagt
    15601 agtaaaaata tttttgtaca taagtaaata gaaattcagt catccatcag atggctggat
    15661 tttttatcaa aaaattaaga aatgagcata tttctttcct tgtctggcgg aattggttat
    15721 aatatacggt acaaaggaat gaatgaatat gtatcgtgtt atagaaatgt acggagattt
    15781 tgaaccgtgg tggttcttag aaggttggga agaagatatt gtagcaagta gaaaatttga
    15841 ccagtattat gatgctctca aatactacaa aacttgctgg tttagattgg aacaagaatc
    15901 gcctctttat aaaagtagaa gcgacttgat gaccattttt tgggacccgg aagaccaacg
    15961 ctggtgtgat gaatgtgatg agtatttaca acaataccat tctttggctc ttttgcagga
    16021 tgagcaggtt atcccagacg aaaaactacg ctcaggctat gaaaaacaaa ccagtcagga
    16081 aaggaatcgt tcttgccgta tgaaattaaa atagagaaaa gtaacttttt tggagttgct
    16141 ttttttattt ttctaactct ttgcgaatag tataggtgag gaggtaagta tggttcaaga
    16201 aattgcacaa gaaatcattc gttcagctcg gaaaaaaggg acgcaggata tctattttgt
    16261 ccctaagtta gacgcctatg agcttcatat gagggtagga gacgagcgct gtaaaattgg
    16321 tagctatgat tttgaaaagt ttgcagccgt tatcagtcac tttaagtttg tggcgggtat
    16381 gaatgtggga gaaaaaagac gtagtcaact gggttcctgt gattatgcct atgaccataa
    16441 gatagcgtct ctacgtttat ctactgtagg cgattatcgg gggcatgaga gtttggttat
    16501 ccgtttgttg cacgatgagg agcaggacct gcatttttgg tttcaggata ttgaagaatt
    16561 aggcaagcag tacaggcaac ggggactcta tctttttgct ggtccggttg ggagtggtaa
    16621 gacgaccttg atgcatgaat tgtccaagtc actctttaaa ggacagcaag ttatgtccat
    16681 cgaagatcct gtcgaaatca agcaggacga catgcttcag ttgcagttga acgaagcaat
    16741 cggcctaacc tatgaaaatc taatcaaact ttccttgcgt catcgaccag atctcttgat
    16801 tatcggagaa attcgtgaca gcgagacggc gcgtgcagtg gtcagagcta gtttgacagg
    16861 tgcgacagtc ttttcaacca ttcacgccaa gagtatccga ggtgtttatg agcgtctgct
    16921 ggagttgggt gtgagtgaag aagaattggc agttgttctg caaggagtct gctaccagag
    16981 attaatcggg ggaggaggaa tcgttgactt tgcaagcaga gattatcaag aacaccaagc
    17041 agccaagtgg aatgagcaaa ttgaccagct tcttaaagat ggacatatca caagtcttca
    17101 ggctgagacg gaaaaaatta gctacagcta agcaaaaaaa tatcatcacc ctatttaaca
    17161 atctcttttc tagcggtttt catctggtgg agactatctc ctttttagat aggagtgctt
    17221 tgttggacaa gcagtgtgtg acccagatgc gtgtgggctt gtctcagggg aaatcattct
    17281 cagaaatgat ggaaagtttg ggatgttcaa gtgctattgt cactcagtta tccctagctg
    17341 aagttcatgg caatctccac ctgagtttgg gaaagataga agaatatctg gacaatctgg
    17401 ctaaggtcaa gaaaaaattg attgaagtag cgacctatcc cttgattttg ctgggttttc
    17461 ttctcttaat tatgctgggg ctacggaatt acctgctccc acaactggat agtagcaata
    17521 ttgccaccca aattatcggt aatctgcccc aaatttttct aggcatggta gggcttgttt
    17581 ccgtgcttgc ccttttagca ctcacttttt ataaaagaag ttctaagatg agtgtctttt
    17641 ctatcttagc acgccttccc tttattggaa tctttgtgca gacctacttg acagcctatt
    17701 atgcacgtga atgggggaat atgatttcac agggaatgga gttgacgcag atttttcaaa
    17761 tgatgcagga acaaggttcc cagctcttta aagaagtcgg tcaagatctg gctcaaaccc
    17821 tgaaaaatgg ccgtgaattt tctcagacga taggaaccta tcctttcttt aggaaggaat
    17881 tgagtctcat catagagtat ggggaagtta agtccaagct gggtagtgag ttggaaatct
    17941 atgctgaaaa aacttgggaa gcctttttta cccgagtcaa ccgcaccatg aatttggtgc
    18001 agccactggt ttttatcttt gtggcactga ttatcgtttt actttatgcg gcaatgctca
    18061 tgcccatgta tcaaaatatg gaggtaaatt tttaaaatga aaaaaatgat gacattcttg
    18121 aaaaaagcta aggttaaagc ttttacattg gtggagatgt tggtggtctt gctgattatc
    18181 agcgtgcttt tcttgctctt tgtacctaat ctgaccaagc aaaaagaagc agtcaatgac
    18241 aaaggaaaag cagctgttgt taaggtggtg gaaagccagg cagaacttta tagcttagaa
    18301 aagaatgaag atgctagcct aagaaagtta caagcagatg gacgcatcac ggaagaacag
    18361 gctaaagctt ataaagaata caatgataaa aatggaggag caaatcgtaa agtcaatgat
    18421 taaggccttt accatgctgg aaagtctctt ggttttggga cttgtgagta tccttgcctt
    18481 gggcttgtcc ggctctgtcc agtccacttt ttcagcggta gaggaacaga ttttctttat
    18541 ggagtttgaa gaactctatc gggaaaccca aaaacgcagt gtagccagtc agcaaaagac
    18601 tagtctgaac ttagatgggc agacgcttag caatggcagt caaaagttgc cagtccctaa
    18661 aggaattcag gccccatcag gccaaagtat tacatttgac cgagctgggg gcaattcgtc
    18721 cctggctaag gttgaatttc agaccagtaa aggagcgatt cgctatcaat tatatctagg
    18781 aaatggaaaa attaaacgca ttaaggaaac aaaaaattag ggcagtgatt ttactggaag
    18841 cagtagtcgc tctagctatc tttgccagca ttgcgaccct ccttttggga caaattcaaa
    18901 aaaataggca agaggaagca aaaatcttgc aaaaggaaga agtcttgagg gtagctaaga
    18961 tggccctgca gacggggcaa aatcaggtaa gcatcaacgg agttgagatt caggtatttt
    19021 ctagtgaaaa aggattggag gtctaccatg gttcagaaca gttgttggca atcaaagagc
    19081 cataaggtca aggcttttac cttgttagaa tccctgcttg ccctcattgt catcagtggg
    19141 ggattactcc tttttcaagc tatgagtcag ctcctcattt cagaagttcg ctaccagcaa
    19201 caaagcgagc aaaaggagtg gctcttgttt gtggaccaac ttgaggtaga attagaccgt
    19261 tcgcagttcg aaaaagtaga aggcaatcgc ctatacatga agcaagatgg caaggacatc
    19321 gccatcggta agtcaaagtc agatgatttc cgtaaaacga atgctcgtgg tcgaggttat
    19381 cagcctatgg tttatggact caaatctgta cggattacag aggacaatca actggttcgc
    19441 tttcatttcc agttccaaaa aggcttagaa agggagttca tctatcgtgt ggaaaaagaa
    19501 aaaagttaag gcaggtgttc tcctctacgc agtcaccata gcagccatct ttagtctttt
    19561 gttgcaattt tatttgaacc gacaagtcgc ccactatcaa gactatgctt tgaataaaga
    19621 aaaattggtt gcttttgcta tggctaaacg aaccaaagat aaggttgagc aagaaagtgg
    19681 ggaacagttt tttaatctag gtcaggtaag ctatcaaaac aagaaaactg gcttagtgac
    19741 gagggttcgt acggataaga gccaatatga gtttctgttt ccttcagtca aaatcaaaga
    19801 agagaaaaga gataaaaagg aagaggtagc gaccgattca agcgaaaaag tggagaagaa
    19861 aaaatcagaa gagaagcctg aaaagaaaga gaattcatag tcaattcaac tataatgcgt
    19921 tgaatccaga atagtccact gtagtttcta gaaaattgct ggaaatggat gttaagctcc
    19981 aattcatttg tttatatctt atttcagttt actatacttt gtgctaaatt aaagatatga
    20041 aacatgattt taaccacaaa gcagaaactt tcgattcccc taaaaatatc ttcctcgcaa
    20101 acttggtatg tcaagcagcc gagaaacaga ttgatcttct atcagacaaa gaaattttag
    20161 atttcggtgg tggcacgggt ctattagcct tgcccctaac ccctagccaa gcaggctaag
    20221 tcagtcactc ttgtagacat ttctgagaaa atgttggagc aagctcgttt gaaagtggag
    20281 cagcaagcaa tcaagaatat ccagtttttg gagcaagatt taccgaaaaa tcccttggag
    20341 aaagagtttg attgccttgc tgttagtcgg gttcttcatc atatgcctga tttggatgcg
    20401 gctctctcac tgtttcatca acatttgaag gaagatggga aactcatcat tgctgatttt
    20461 accaagacag aagctaatca tcatggattt gatttagctg aactggaaaa caagctaatt
    20521 gagcatggtt tttcatctgt gcatagtcag attctctata gtgctgaaga cctgtttcaa
    20581 ggaaatcact cagaattctt tttaatagta gcccaaaaat cactcgccta gtcagggagt
    20641 gatttttcta taaggatgga aaaaagaagg gaaatttggt aagataggaa tatggatttt
    20701 gaaaaaattg aacaagctta tacctattta ctagagaatg tccaagtcat ccaaagtgat
    20761 ttggcgacca acttttatga cgccttggtg gagcaaaata gcatctatct ggatggtgaa
    20821 actgagctaa accaggtcaa ggagaacaat caaaccctta agcgtttagc actacgcaaa
    20881 gaagaatggc tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac agaacccttg
    20941 caggccaatc accagtttac accggatgct attgctttgc ttttggtgtt tattgtggaa
    21001 gagttgttta aagaggagga aattactatc ctcgaaatgg gttctgggat gggaattcta
    21061 ggcgctattt tcttgacctc gcttactaaa aaggtggatt acttgggaat ggaagtggat
    21121 gatttgctga ttgatctggc agctagcatg gcagatgtaa ttggtttgca ggctggcttt
    21181 gtccaaggag atgccgttcg cccacaaatg ctcaaagaaa gcgatgtggt catcagtgac
    21241 ttgcctgtcg gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt tgcttctagc
    21301 caagaacata cttacgccca tcacttgctc atggaacaag ggcttaagta cctcaagtca
    21361 gacggatacg ctatttttct agctccgagt gatttgttga ccagtcctca aagtgatttg
    21421 ttaaaagaat ggctgaaaga agaggcgagt ctggttgcta tgattagtct gcctgaaaat
    21481 ctctttgcta atgccaaaca atctaagact atttttatct tacagaagaa aaatgaaata
    21541 gcagtagagc cttttgttta tccacttgct agcttgcaag atgcaagtgt tttaatgaaa
    21601 tttaaagaaa attttcaaaa atggactcaa ggtactgaaa tataaaatag attttgttat
    21661 aatagttgaa aacgcttaaa aaggggtatc atgttatgac aaaaacaatt gcaatcaatg
    21721 caggaagttc aagtttgaaa tggcaattat acttaatgcc agaagaaaaa gtattggcga
    21781 aaggtttgat tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa tttgacggcc
    21841 gttctgaaca acaaattttg gatattgaaa atcatataca agccgttaaa attttattgg
    21901 atgacttgat tcgtttcgat attatcaagg cttatgacga gattacaggt gttggacatc
    21961 gtgttgttgc tggtggagaa tatttcaaag aatcaacagt tgttgaggga gatgttttag
    22021 aaaaagttga agagttgagt ttgttggctc ctctacacaa cccggccaat gcagcaggtg
    22081 ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt agttgttttt gatacttcct
    22141 tccacacaag tatgccagag aaagcttatc gctaccctct accaacaaaa tattacacag
    22201 aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta gcaggagaag
    22261 ctgcaaaact cttgggacgt ccattagaag acttgaagtt aattacctgt catattggta
    22321 acggaggctc aattacagct gtgaaagccg gcaaatctgt agacacttct atggggttca
    22381 ctcctcttgg tggtattatg atgggaacgc gtacagggga tattgatcca gctatcattc
    22441 cttatttaat gcaatataca gaggatttta acacaccaga agatatcagt cgtgttctta
    22501 accgtgaatc aggtcttttg ggagtttctg ctaattctag cgatatgcgc gatatagaag
    22561 cagctgtagc agaagggaat cacgaggcta gcttggctta tgaaatgtat gttgaccgta
    22621 tccaaaaaca tatcggtcag taccttgcag tgctaaatgg agcagatgcc attgttttca
    22681 cagcaggtgt cggtgaaaat gcagagagtt tccgtcgtga tgtaatctca gggatttcgt
    22741 ggtttggttg tgatgttgat gatgaaaaga atgtctttgg cgttacagga gacatctcaa
    22801 cagaggcagc taaaatccgt gtcttggtta ttccaacaga tgaagaatta gtcattgccc
    22861 gtgacgttga acgcttgaaa aaataagtga aactaaaaaa atattcaata caaggagttg
    22921 ggaaagttat ttttccagct tctttttctg atgaaattgt ccaaaacctt gctatgattg
    22981 gcttttttga aaaatatggt ataatagtag taatttaata gatggagttg agttttgaag
    23041 aaaaactttc gtgtaaaaag agagaaagat tttaaggcga ttttcaagga ggggacaagt
    23101 tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc agaaaaaccg ttttcgagta
    23161 ggtctatcag ttagcaaaaa actggggaat gccgtcacta gaaatcaaat taagcgacgg
    23221 attcggcata ttatccagaa tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc
    23281 attgctcgaa aaggagtcga aaccttggga tacgcagaga tggagaaaaa tctactccat
    23341 gtattaaaat tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt
    23401 gactagtttg ctaggactgt ctctgttaat catgacagcc tgtgcgacta atggggtaac
    23461 tagcgatatt acagccgaat cggctgattt ttggagtaaa ttggtttact tctttgcgga
    23521 aatcattcgc tttttatcgt ttgatattag tatcggagtg gggattattc tctttacggt
    23581 cttgattcgt acagtcctct tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat
    23641 gcaggaagct cagccacgca ttaaggcgct tcgagaacaa tatccaggtc gagatatgga
    23701 aagcagaacc aaactagagc aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca
    23761 gtcagactct ctttggccga ttttgattca gatgccggtt attttggccc tgttccaagc
    23821 cctatcaaga gttgactttt taaagacagg tcatttctta tggattaacc ttggtagtgt
    23881 ggatacaacc cttgttcttc cgattttagc agcagtattc acctttttaa gtacttggtt
    23941 gtccaacaaa gctttgtctg agcgaaatgg cgctacgact gcgatgatgt atgggattcc
    24001 agtcttgatt tttatctttg cagtttatgc gccaggtgga gtcgccctat actggacagt
    24061 gtctaatgct tatcaagtct tgcaaaccta tttcttgaat aatccattca agattatcgc
    24121 agagcgcgag gccgtagtac aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa
    24181 gaaaaaggct cagaaaacga aataaataag gaggaatctg gtagtggtag tatttacagg
    24241 ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa ttagatattc caagaatgaa
    24301 ggctcatatc aaagtcattt ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa
    24361 accagcccaa gtggatattg aagcgattag tgaaacgact gttgtcaaag caaatcaaca
    24421 ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga agacggttag
    24481 tgaagaaacc gttgaccttg gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg
    24541 tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa catgaaagac atgccagcac
    24601 tatcttagaa gaaactggtc acattgagat tttaaatgaa cttcaaatcg aggaagcgat
    24661 gagggaagaa gcaggcgctg atgaccttga aactgagcaa gaccaagctg aaagtcaaga
    24721 actagaagac ttgggcttga aagttgaaac gaactttgat attgaacaag tagctacgga
    24781 agtaatggct tatgttcaaa cgattattga tgacatggat gttgaggcta cactttcaaa
    24841 tgattataac cgtcgtagca tcaatctaca aattgacacc aacgaaccag gtcgtattat
    24901 cggctaccat ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt atctttacaa
    24961 ccgctattcc agaaccttct acgttacaat caatgtcaat gattatgtcg aacaccgtgc
    25021 agaagtcttg cagacctatg cgcaaaaatt ggcgactcgt gttttggaag aagggcgcag
    25081 tcataaaaca gatccaatgt caaatagcga acgcaagatt atccatcgta ttatttcacg
    25141 tatggatggc gtgactagtt actctgaagg tgatgagcca aatcgctatg ttgttgtaga
    25201 tacagaataa gtaaaatcag gtttatcctg attttttgct agttagagga ggttaaactg
    25261 atgttgaata agataagaga ctatttagac tttgctggtt tgcagtaccg taatcctgat
    25321 aaagcgggag cagagcgaga gaagatgctg gcattccgcc acaaaggaca agaggcccga
    25381 aaggttttta cagaactggc caaagccttt caagcaagcc atccagaatg gcaactccaa
    25441 cagactagcc agtggatgaa tcaggcccag cgtttgagac cacatttttg ggtttatcta
    25501 cagagagacg gacaagtgac agaacctatg atggccttac gtttgtatgg gacatctact
    25561 gactttggaa tttctttgga agtcagtttc atcgaacgta agaaggatga gcaaacactg
    25621 ggcaagcagg ccaaagtttt agacattcca accgttaaag ggatttatta tctaacctac
    25681 tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag
    25741 aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa
    25801 aattcgtctg aagaagaaat cgtagaaggc ttattgaagt cttattctaa aattcttccc
    25861 tattatctag ctacgagaaa ataagataat ttgtaaaaca tcataaatca tacagtccaa
    25921 gagtgaacag tccgctgtgt aattcttggt ctttttgttt gcgctttcgc attatataat
    25981 aaacttacaa aaacaattca aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa
    26041 ttggttttct agcaatattt tgcagaatcc cgcatttttc gtaggtttat tggtgttgat
    26101 aggatatgca cttttgaaaa aacctgccca tgacgttttt tcagggtttg ttaaagcaac
    26161 agtagggtat atgttgctta acgtgggtgc tggtggtttg gttacaacct ttcgtccaat
    26221 cttagcagct cttaactaca aattccaaat tggtgcagcg gttatcgacc cttactttgg
    26281 acttgctgca gcaaacaaca aaattgtagc agagtttcca gattttgttg gaactgcaac
    26341 tacagctcta ttgattggtt ttggaataaa tatcttgctc gtagctcttc gaaagattac
    26401 gaaggtaaga accctcttta ttactggtca catcatggta caacaagctg caacagtatc
    26461 tcttatggtt ctattcttag taccacaatt gcgcaatgct tacggtacag cagcgattgg
    26521 tatcatctgt ggactttact gggcagttag ttcaaatatg actgttgagg caactcaacg
    26581 cttgactggt ggtggcggat ttgcgattgg tcaccaacag caatttgcaa tctggtttgt
    26641 agataaagta gcaggacgct ttggtaagaa agaagaaagt ttagacaatc ttaaattacc
    26701 taagttcctc tcaatcttcc acgatacagt tgttgcatct gctaccttga tgctcgtatt
    26761 cttcggagcc attcttttaa tcttgggtcc agacattatg tctaataaag aagtcatcac
    26821 ttcaggaact ctattcaatc ctgctaaaca agatttcttt atgtacatta tccaaacagc
    26881 ctttaccttc tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga
    26941 gttgacaaac gccttccaag gtatttcaaa caaattgttg ccaggttcat tcccagcggt
    27001 tgacgttgca gcttcttatg gatttggttc tccaaatgct gtcttgtcag gatttacctt
    27061 tggtttgatt ggtcaattga ttacaattgt tttgctcatc gtctttaaaa atccgattct
    27121 tattattaca ggatttgtac cagtgttctt tgacaatgca gccattgcgg tctacgctga
    27181 taaacgcggc ggatggaaag cggctgttat cctttccttt atatcaggtg tccttcaagt
    27241 tgctctagga gctctttgtg tggcccttct cgatttggca tcttatggtg gctaccatgg
    27301 aaatatcgac tttgaattcc catggcttgg atttggatat atcttcaaat accttggtat
    27361 tgttggttat gtacttgtgt gtctcttctt gcttgttatt cctcaacttc aatttgccaa
    27421 agcaaaagat aaagagaaat attacaacgg tgaagttcaa gaagaagctt agtatctaga
    27481 aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa
    27541 tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct taatcaaaca gattttacag
    27601 tcaattcatg cagtgtcggt gaagctaaag gtttagcagt aggatatgac atcgtaatcg
    27661 cttctcttca tttgattcaa gaattggaag ggcgaactaa tgggaagtta attgggcttg
    27721 ataacttgat ggatgataaa gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg
    27781 ttggaggggg ctggacagaa actgagagtt atcgtttctg tccttctccc tctttaaata
    27841 aaggaggcag atatgaattt aaaacaagct ttaattgaca atgactcgat ccgactaggt
    27901 ttagaggcta acaattggaa agaagcagtc aaggtagcag tagatccctt aattgaaagt
    27961 ggggcaattt tgccagagta ttacgatgct atcattgaat cgactgaaga gtatgggcct
    28021 tactatatct tgatgccagg tatggctatg ccccacgcta gacctgaagc aggtgtgcaa
    28081 agtgatgcct tttcattgat taccttacaa aatcctgttg tattttcaga tgggaaagag
    28141 gtatctgttt tgttggcact agcagcaaca agttcaaaaa ttcacacaag tgtagccatt
    28201 ccacaaatta ttgccctatt tgaattagaa gattctattg cacgtttaca ggcttgccag
    28261 actaaagaag atgtcttggc tatgattgaa gaatctaagg atagccctta tctcgaagga
    28321 ttggatttgg aaagttagaa agaggaataa agaaatgaca aaaagaatac ctaatttaca
    28381 agttgcatta gaccattcag acttgcaagg agcgattaaa gcagctgttt ctgttggtca
    28441 ggaagtagat attatcgaag ctggaactgt ttgcttgctt caagttggaa gtgaactggc
    28501 tgaagtcttg cgtagccttt tcccagataa gattattgtg gcagacacaa aatgtgctga
    28561 tgctggtgga acagttgcta aaaataatgc ggttcgtgga gcagactgga tgacttgtat
    28621 ctgttgtgca accatcccta ctatggaagc agctctaaag gctatcaaga ctgaacgagg
    28681 agaacgaggc gaaatccaga tcgagcttta tggcgattgg acttttgaac aagctcagct
    28741 ttggctagat gcaggtatct cacaagctat ttatcaccaa tctcgtgatg ctcttcttgc
    28801 tggtgaaact tggggtgaaa aagaccttaa taaggttaaa aaactcattg acatgggctt
    28861 ccgtgtatct gtaacaggtg gtctagatgt agatactctc aaactctttg aaggtattga
    28921 tgtctttacc tttatcgcag gtcgtggaat tacagaggct gtggatccag caggagcagc
    28981 gcgtgccttc aaggatgaaa tcaaacgaat ttgggggtaa atcatggtac gtccaattgg
    29041 aatttatgaa aaggcaaccc caacacactg tacttggcta gaacgtttaa attttgccaa
    29101 ggagttaggc tttgattttg tcgagatgtc tattgacgaa cgtgacgagc gtttagcaag
    29161 acttgactgg agtaaggaag aacgcttgga agttgtcaaa gcaatctatg aaactggtgt
    29221 tcgtattcct tctatctgtt tttcaggcca tcgtcgctac ccattgggtt caaaagatcc
    29281 agttctagag gaaaaatctc tagaactcat gaaaaaatgt atcgaattag ctcaagactt
    29341 gggagttcgt acgattcaat tagctggtta cgatgtttac tatgaggaaa agtcacccca
    29401 gacacgccaa cgttttatca aaaatttgag aaaagcctgt gactgggctg aagaagctca
    29461 ggtggtactt gctattgaaa ttatggatga tcctttcatc agtagcatcg aaaaatattt
    29521 ggctatagaa aaagagattg actctccctt cctctttgta tatccagata ttggtaatgt
    29581 gtctgcatgg cataatgata tctatagtga gttttatctt ggtcatcatg ccatcgcagc
    29641 tctccatctc aaggatactt atgcagtgac agaaagttca aagggccagt tccgagatgt
    29701 acctttcggg caaggttgtg tcaaatggga agaagctttc gatattttaa aggaaaccaa
    29761 ttataatgga cctttcctaa tcgaaatgtg gtctgaaaat tgtgaaacag tagaagaaac
    29821 acgcgcagcc attcaagagg cgcaagcttt tctctatcca ctcattaaga aagcaggttt
    29881 gatgtaagat gaatcaagta atcaatgcta tgcgtaaacg agtctgtgat gccaatcaat
    29941 cattgccaaa acatggactt gtcaaattta cctgggggaa tgtatctgaa gttaatcgcg
    30001 aactcggtgt cattgttatc aaaccatcag gcgtggatta tgacgaattg acacctgaaa
    30061 acatggtagt gactgatcta gatggtaaga tcctagaagg ggatttaaga ccatcttccg
    30121 acctcccaac tcatgtgcaa ttatataaga cttggtcaga aattggtagt gtggttcaca
    30181 cccattcgac agaagctgtt ggttgggctc aggcaggtcg tgatattcct ttctacggaa
    30241 caacccatgc agattatttc tacggttcaa tcccttgcgc ccgtagtttg accaaggacg
    30301 aagtagaagt ggcctatgaa aaagatactg gcctggttat cgtagaagag tttgaacatc
    30361 gcggacttaa cccggttgaa gtaccaggaa ttgttgtacg caatcacggt ccattcacct
    30421 ggggcaaaaa tccagagaat gctgtttatc actctgtcgt actagaggaa gtatcaaaga
    30481 tgaatcgctt tacagaacaa atcaatccaa gagttggacc tgctccccag tacatactag
    30541 aaaaacacta ccaacgtaaa catggaccaa atgcttatta tggtcaaaag taagaacgat
    30601 gaaggaggag aaaaagataa atttagctcc tctttttaca tttgattttt attgagagta
    30661 aagttggagt tgaagtaatt ttaaaagatt ttttagaaat agcgcttgat atatatatgg
    30721 taaaataaaa agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg
    30781 gataaggcaa gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt
    30841 atggcgattt cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa
    30901 atcaatgctg ctctgggtga cgaggcgctt cacatcatta gtattccacg aattggtatt
    30961 cacttaacgg aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac
    31021 gattatatca tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct
    31081 aaagaacgta ttacgattga aaaattgata gagttaacag aggtatctag gaatactgtt
    31141 ctcaatgatt tgaatagtat tcgttatcaa ctaactttgg aacaatatca ggtgatcttg
    31201 caagtgagca agtcacaggg atacaacctt catgcccacc ctcttaataa aattcagtat
    31261 cttcaatcgc ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta
    31321 gaagataaga tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa
    31381 ttttttaagg aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat
    31441 gaaataactt ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa
    31501 cagtatcaag aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata
    31561 gagtatcagg tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg
    31621 tcaggacttg aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat
    31681 attcatgcag aaagtgatga ttttcggcaa ttaaaacttg ctttagaaga atttatctgg
    31741 tattttgaat cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg
    31801 atgatccact gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct
    31861 ctaacaaaac aaattcgatc caagtatgga gaattatttt tagtcactag aaaatctgcg
    31921 gaaattttag aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg
    31981 attcatattg gaggattttt aaaatataca ccatcatctc aaaaaaatat gaaaaaagtt
    32041 tatctcgttt gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt
    32101 tattttccaa atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa
    32161 gatattgcac aagttgatgt agtgattact actaatgatg atttggatag cagatttccg
    32221 attttaaggg ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt
    32281 aaacacaata tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt
    32341 tcgtcttata ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt
    32401 ataaatcaag aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga
    32461 tgaacacaaa cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat
    32521 ctcaaagata acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca
    32581 agagagtcat ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc
    32641 gaagaagaag tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact
    32701 tggattaaga caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa
    32761 tcaaccaaaa aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt
    32821 cgtaagctgc aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac
    32881 gaactagact attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct
    32941 gcagcaattc tcaataatcc taagttagag catgttaagt ttatcggtcc ttaccactgt
    33001 ggacgaatct gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt
    33061 gacactatcg aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc
    33121 ttggtaactc tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct
    33181 gttacagatg aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga
    33241 accatctatc atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac
    33301 tttaaaattg atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa
    33361 atgacatcta tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca
    33421 gttcactatg atatctggtc taacttcatg gcttctacta atgagattct agaactttgg
    33481 aaaatacgaa aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt
    33541 aagtacactt atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat
    33601 gattgttttg aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt
    33661 ctttggaggt tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga
    33721 atgtgtgatt caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta
    33781 atgcaattcg ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg
    33841 tggttatggg agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc
    33901 cagctcaacc aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa
    33961 tgctccttta tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta
    34021 agagtttccg tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag
    34081 ggattgatgc tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc
    34141 aagcagaacg tttcttggca gccaaatata accgtgaagg ttacaatatc tttgaccact
    34201 atacttacgt tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt
    34261 catacgcagg cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca
    34321 acttggatgg tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct
    34381 acggttggca tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta
    34441 tcgaaacagc aaaagcttca ggcaagccat ctttgattga agtgaagacg gttattggat
    34501 acggttctcc aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg
    34561 aaactgcatc aactcgtcaa gccctcggtt gggactacga accatttgaa attccagaac
    34621 aagtatatgc tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt
    34681 ggactaaatt agttgcagat tataaagaag ctcatccaga actggctgca gaagtagaag
    34741 ccatcatcga cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa
    34801 atggtttttc tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta
    34861 aattaccaac ctttctaggt ggatcagctg accttgctca ctcaaacatg acttatatca
    34921 agacggatgg acttcaagac gatgccaatc gcttgaatcg taacattcag tttggtgttc
    34981 gtgaatttgc aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat
    35041 acggtggaac tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct
    35101 tacaaggact tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg
    35161 gtccgactca tgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg
    35221 ttttccgtcc agcagatgcg cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg
    35281 agaaaacacc aactgccctt gtcttgacac gtcaaaattt gactgttgaa gatggaacag
    35341 acttcgacaa ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata
    35401 ccatcttgat tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg
    35461 ctagtcaagg cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac
    35521 aagatgcagc ttacaaggaa gaaattcttc caaatgcagt ccgccgtcgt gttgcagtcg
    35581 aaatgggtgc aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta
    35641 ttgatacctt cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg
    35701 tagaaaatct tgtaaaagtt gttcgaaact tgaaataatc ctaaaaatca gggcgtaagc
    35761 tctggttttt cttaccagaa aagtaaggta caatcttgta aaagtagctg aaatttgata
    35821 tagtagtcct atgtaaaaga caaaggagaa aacaatgaat ccaaatatta cttttttaat
    35881 catgcttgta ggtatgatgg ccttgatgtt ctttatgcaa cgttctcaaa agaaacaagc
    35941 tcaaaagcgt atggaaagct taaataaact acaaaaaggc tatgaagtga ttacaatcgg
    36001 tggactttat ggaacagtcg atgaagtaga tacggagaag ggaacaatag ttcttgacgt
    36061 agatggagtt tacttgactt ttgaactagc tgctatcaag acagtattac cgctgaaaga
    36121 aacagcttca ctagaaggcg caattgaaaa ataagacggg atcacgaact cccgttttct
    36181 ataaaagaaa ggaaatggga tgaaaaaatt agtctttgtc tgtctgggaa atatttgccg
    36241 tagccctatg gccgagtttg ttatgaaatc aatgacagat aactacgaaa tccaaagtcg
    36301 agcaacttcc tcttgggaac atggcaatcc gattcataag gggactcagg gaatttttca
    36361 agagtatgag attccttatg acaagaacaa gacatcgctt cagattagta aggaagattt
    36421 tgaagccttt gattatatta tcggaatgga cgcttcaaat atttctgact tacgtcagat
    36481 gtgtccagta gactgtcaag ataagattta ctcattttca tctgaaagtg ttccagaccc
    36541 ttggtataca ggagattttg aagaaaccta tcgacgtgtt caagagggct gtcaagcttg
    36601 gttagaacgt ttagaaaagg agagttaagg tggaaaatct tacgaatttt tacgaaaagt
    36661 atcgtgtcta tctgactcgt ccacgtttag agcttttggc agtagttacc attgttttct
    36721 gtgctgtact cgtctttttt ctaaatattc caggaaaagg tgtcttaaaa ctcgataatg
    36781 gaacgattgt ttatgatggc agtcttgtcc gcggtaaaat gaatggccaa ggtaccatta
    36841 ccttccaaaa tggagaccaa tatacaggtg gcttcaacaa tggagccttc aacggaaaag
    36901 gtacctttca atctaaagaa ggctggacct acgaaggtga ttttgtaaat ggtcaggctg
    36961 aaggaaaagg gaaactaaca acagaacaag aagtcgttta tgaaggaact tttaaacaag
    37021 gcgtttttca acaaaaataa agcctcctta tcaaaggagg tattattaga atcgcaaggt
    37081 aagcgcttac ctgcaaatcc ctttctttcc aaatccctct tccaagcaag tttgtgaaat
    37141 aaaaaatatt tgaaataaat ttcacaaact tcaaagataa aacctgataa gaaaagaaaa
    37201 tgagaaaagt ttcacaagag tttaaaaatt ctttgtgaaa tgtttatgaa actgtttaca
    37261 aagttgaaat aatgcgttat aataaacttg tgaaaaaatt aacaaaggat atcgttcctt
    37321 gaaagctatg gaggaaaata tggctgataa aaaaactgtg acaccagagg aaaagaaact
    37381 cgttgctgaa aaacacgtag atgagttggt tcaaaaagct ctagttgccc ttgaagaaat
    37441 gcgtaaattg gatcaagaac aagttgacta catcgttgcc aaagcatcag tagcagcttt
    37501 ggatgcccac ggagaattgg ctttacatgc ctttgaagaa acaggacgtg gtgtatttga
    37561 agacaaagca actaagaact tgtttgcctg tgaacacgta gtaaacaaca tgcgccacac
    37621 taagacagtt ggcgttatcg aagaagacga tgtaacagga ttgactctta ttgctgaacc
    37681 agttggtgtt gtttgtggta ttactccaac aacaaaccca acatcaacag caatcttcaa
    37741 atcattgatt tcattgaaga cacgtaaccc aatcgtcttt gccttccatc catcagcaca
    37801 agaatcatct gctcatgcag ctcgtatcgt ccgcgatgca gctatcgcag ctggtgctcc
    37861 tgaaaactgt gtgcaatgga ttactcaacc atctatggaa gcaacaagtg cccttatgaa
    37921 ccacgaaggt gttgcgacaa tccttgcaac aggtggtaat gccatggtta aggcggctta
    37981 ttcatgtggt aaaccagctc ttggggtagg tgccggaaac gttccagctt atgttgaaaa
    38041 atcagcaaac attcgtcaag cagcacacga tatcgtcatg tctaaatcat ttgataacgg
    38101 tatggtctgt gcatctgaac aagcagttat cattgataaa gaaatttacg atgaatttgt
    38161 agcagagttc aaatcttacc acacttactt tgtaaacaaa aaagaaaaag ctcttcttga
    38221 agagttctgc ttcggcgtca aagcaaacag caaaaactgt gctggtgcaa aattgaacgc
    38281 tgacatcgtt ggtaaaccag caacttggat tgcagaacaa gcaggattta cagttccaga
    38341 aggaacaaac attcttgctg cagaatgtaa agaagttggc gaaaatgagc cattgactcg
    38401 tgaaaaattg tcaccagtta ttgcagtttt gaaatctgaa agccgtgaag atggtattac
    38461 taaggctcgt caaatggttg aatttaacgg tcttggacac tcagcagcta tccacacagc
    38521 tgacgaagaa ttgactaaag aatttggtaa agctgttaaa gctattcgtg ttatctgtaa
    38581 ctcaccttct acttttggtg gtatcgggga cgtttacaat gccttcttgc catcattgac
    38641 acttggatgt ggttcttacg gacgcaactc agttggggat aacgttagtg ccattaacct
    38701 cttgaatatc aaaaaagtcg gaagacggag aaataacatg caatggatga aacttccttc
    38761 aaaaacatac tttgaacgtg attcaattca ataccttcaa aaatgtcgtg acgttgaacg
    38821 tgtcatgatc gttactgacc atgccatggt agagcttggt ttccttgatc gtatcatcga
    38881 acaactggac cttcgtcgca ataaggttgt ttaccaaatc tttgcggatg tagaaccgga
    38941 tccagatatc acaactgtaa accgtggtac tgagattatg cgtgccttca aaccagatac
    39001 catcatcgca ctcggtggtg ggtctccaat ggatgctgcc aaagtaatgt ggctcttcta
    39061 cgagcaacca gaagtggact tccgtgacct tgtccaaaaa ttcatggata tccgtaaacg
    39121 tgccttcaag ttcccattgc ttggtaagaa gactaaattc atcgcgattc caactacatc
    39181 tggtacagga tctgaagtaa caccatttgc cgttatctct gataaagcaa acaaccgtaa
    39241 atacccaatc gctgactact cattgacacc aactgtggca atcgtagatc ctgctttggt
    39301 attgacagtt ccaggatttg ttgctgctga tactggtatg gacgtattga ctcacgcgac
    39361 agaagcatac gtatcacaaa tggctagtga ctacactgat ggtttagcac ttcaagccat
    39421 taaattggtc tttgaaaatc tcgaaagctc agttaagaat gcagacttcc actcacgtga
    39481 gaaaatgcat aacgcttcaa caatcgctgg tatggccttt gccaatgcct tcctaggtat
    39541 ttctcactca atggcccata agattggtgc gcaattccac acaatccacg gtcgtacaaa
    39601 tgctatcttg cttccatacg ttatccgtta caacggtaca cgtccagcta agacagcaac
    39661 atggcctaag tacaactact accgtgcaga tgaaaaatac caagatatcg cacgcatgct
    39721 tggacttcca gcttctactc cagaagaagg ggttgaatct tacgcaaaag ctgtctacga
    39781 actcggtgaa cgtattggga tccaaatgaa ttttagagac caaggaattg acgaaaaaga
    39841 atggaaagaa cattctcgta aattagcctt cctggcttat gaagaccaat gttcaccagc
    39901 taacccacgt cttccaatgg tagaccatat gcaagaaatc atcgaagatg catactatgg
    39961 ctacaaagaa agaccaggac gccgtaaata attgtttatc agtctagaag caagacaaaa
    40021 actcaatttg agggaaagat ccagtaattt ttctatgata aaaggcatcc tatcaaggtt
    40081 tttgaacacc tgataggatg cctttttatg atattgaggc ctttttgccc tttttgaaaa
    40141 actagaatag aaacaaaata tataatagat tgaaactaga atagtacata tctgcttcta
    40201 aaacattgtt agaattcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
    40261 tgatatataa aaaatatagt atagtagact gaatctaaaa tagtacgaaa caattgctaa
    40321 aacatttata gaaattaatt ttacttttct gatagagttg ttcacatctt atttcaattc
    40381 actatagttt aatttaagag tagtatttac taaggcccaa ttaaaatcaa agagcaaact
    40441 agaaaacgag tgccattcag ctcaaaacac tgatttgaga ttgcagataa gactagcccc
    40501 ctcattaaca gatttacgat aaaacgatga caaggtgtgt tgctttttga tttctaaaga
    40561 gtataatgat agatctctat aaaataagtg cgaaggaaat gagcttttat agtcctttcg
    40621 ttttaaaata ctatctcaga tattcttata tcgacaagaa gtttttgagt cattccctca
    40681 tcatacatat taaataaata gtggctcatt caatttttca ctagaataat aagctagtat
    40741 agtaaactga aataagatat aaacaaataa attggagctt aacatccatt tccagcaatt
    40801 ttttagaaac tacagtggac tattctagat tcaacatatt ataaaaacta gagtaaaaga
    40861 aaaggattgg atcttgtgta atgcaggatc caatcctttc aatcattttg tccaactttt
    40921 ggaggttcct acaatgtagt cgtcattaat aaagacagat gggaatgaca gtgttcctat
    40981 ttattttgat agagatcgat gaattcttta gatagcaact gaataatctc tgttgaagcc
    41041 atttggtctt ctgcatgcat aaatagcaag gagaatccta ttttttctcc agtagcttct
    41101 ttttgtatga gattagagtg aatcttgtgc gcttctacta aggagtcttc cgcttcttca
    41161 actttaattt tcgcttcttt taaatttcct gccttagcta gttggatggc ttcaataaag
    41221 gatgatttgg ctgctccact attggcaatg agctgaaaac agatatattc catttcttct
    41281 gtcatcttat ttctcctatc catgcaagtg cttgttccag aacttttgct ccattcatca
    41341 ttccgtaatc ccgcatatca atggtatcta cagggatatt tcctgcaatt tctttcacag
    41401 caagtaactc ataacgaatt tgtggcccaa ttagaatgac atctgcttca tggatattct
    41461 ttttagcttc tgtcattgat tttgcttgga tagagatttc aatcccacgt tcagtcgcac
    41521 tttgttgcat ttttttaaca agcatacttg tcgacattcc cgcattacat actaataaaa
    41581 tttgtttcat aatcttaacc ttccatttct tgttcaacaa ctttgtcatt aactttgata
    41641 aatggaatgt atagaagaac tccaagtgca aagatgatga attgaactag aactgctctc
    41701 acgtcccctg ctgttgctaa ccatgcattt aagaatactg gtgtagtcca aggaacttgt
    41761 ataaatgcag gactcatgaa ttctgtaact gttgctaagt agctgattaa aataccaagg
    41821 actggaactg tgataaatgg aatagctaat gaaatgttat aaacgattgg gtaaccgaat
    41881 aatactggtt cattgatatt gaagatacca ggtccaaaag ataatttagc cacgttttta
    41941 gagacagcat tgcgactcac taagaatgtt gctattaata aacataatgt agatccacta
    42001 ccacccatta aagcgaatgt ttgtatttgt gataggttga tgatgtgtgg aatggcttgt
    42061 ccattatttg ctgcagtgat gttttcagta atgttaatta atagtaatgg ttctaggatg
    42121 gcactgtaaa taactgcttg gtgaatacca aatagccata acatatttcc taaagagtaa
    42181 ataataatga ccccgattaa gcttgtacca atatgacgaa ttggttcttg aataaagatt
    42241 gtaatgattg agattaagtt cattccagtt atattgaata ataatgctga aacaacccca
    42301 aataaggaga tgacggtcat gactggaagt aatacgctaa atgatctact aacagctggt
    42361 ggaatatttt caccaaggtt catttgtaaa gctttaacgt ttgataattc aatgaataat
    42421 tctgttgcaa taatcgctac gataaccccg gcgaacattg cgcctgtacc tgtgttgttg
    42481 aatgaaagaa cacctgaaat gtttaccgca tcttttgctc cgtcaggaac tacagaaact
    42541 gtatttggca tcatcacaat taaagaaact aatgatagca ttgatgctgc taacgggttt
    42601 tcgaaatctc tgtttttagc taagaaataa ccaaccatta cagcaataat catacctgaa
    42661 atacttaaag taccgtttgc aattgttatt ccccaatatt ggaatcttgt taatgtatcc
    42721 ccttggaaaa tccacttaaa taccgtgttg ttcaaaagaa cgattaaacc tgccaaaata
    42781 tataatggca ttactgttac gaatgcatct cttagggttt ttaaatgaat ttggttccct
    42841 agtttaccag caaaggtggc aaattggtct ataaattttg agtttttcat atgaatatct
    42901 cctctcttta ctcctcaatt tctacttcaa aaccgttatt gtcacttact ttgcgatacc
    42961 atcttcctga gctcttgatg gttctctttt gcgtttctaa atcaacggag ataaatccgt
    43021 aacgattctt atatgcatta ttccaagacc agcaatcaaa tgcggtccaa gcgtggtatc
    43081 caaaacagtg acttccctct tcaatggctt tatgtagcca tcttaaatgt tcttcataaa
    43141 attcaatacg atacacgtca tcgataactc cattttcatc gataaaccgt gcttcgtttt
    43201 caacacccat tccgttttca ctgataaacc atgggatatt accatattct tccttcacaa
    43261 tcatagcaat atcataaatg gctttcggaa aaatttccca accacgatat ggattcatgc
    43321 gacgctccag ccattcatac tctttgaagt attggtctgg catccagggc gtctgatatt
    43381 cctcaggatt tgcttgtgct tgaacacgtt ttggatggta gtagtttact ccaagaaaat
    43441 caaccgtatt tgatttcatc agttgaagct ctttttcggt atgactccat aacacgccat
    43501 ctctctctag ctgttttacc aatctttctg ggaaagttcc tttaacagct ggattcaaga
    43561 agactttgtt aaagaagtca tctgtaaatc gacttgcttc taagtcttct ggagaattac
    43621 ttcttggata agcaggtgtc aagtttaaaa taatcccaat ctttccatca agttctaatg
    43681 agcgatatag ttgaatcact tttgcactag caaggtttag attatagatg acttgtacgg
    43741 cctcttttcc ctttcctttt agatttggat aatggaaagc atataagtat cctgcttctg
    43801 gaatgaccat tggctcattg aaagttgtcc agtaatgaac cttatctcca aagcatgtga
    43861 aagcagtctt ggcaaacttc acgaataact ccactacatg tttgctttcc caaccaccgt
    43921 atttttgaag aagttccact ggtaaatcaa aatgatgtaa attcatcaca agatccatct
    43981 ggttcttttt agcttcttca atgatggcat tgtagaaagc aatacctttt ggatcaggct
    44041 cacctgtctc taaattcttg atgagtcgac tccattggat ggaagtgcgg aaagaattaa
    44101 ctccaatttc cttcatcaag tggaaatctt cggtgtaagt atgaaaaaag ttactggcta
    44161 caagaggtcc gacattatcg aaaaaatctt ctggatgcgt tttgaaccag tagtccatca
    44221 cattttcatg tacttttcca tattgacctt ctgtctgagg tccgctagaa gcagctcccc
    44281 accaaaacag attaggaaat actaactttt ttttcatgct caacctcctt tctattattc
    44341 tatacataaa gtatagcata attttaaaat ttgtctagac atttttaaaa attgttgata
    44401 catatcttgt gtttgcctca tttcttgaac tttgttatac tactattaga atattataga
    44461 gagaaccaga ttaaatgagt acaaaatatt tatttattta caatgagatt cgtgaaaaga
    44521 ttctttgtaa taaatatacc atgaacgaac aattgcctga tgaaatgaca ttagctaaac
    44581 agtttgcctg tagtcgaatg acgatcaaaa aagctttaga cttgttagtt tctgagggct
    44641 taatttttag aaaacgtggg cagggaacct ttgttctctc tcgtggcagc tcaaaaagaa
    44701 aattaatcgt tccagaaaga gatatccggg gactgacaaa aatatctgaa gatgctcatt
    44761 ctacaattga ctcgaggatt attcacttca aattagaatt tgcaaatgaa tttttagcag
    44821 aaaaactaca ggtcgctttg cagagtccag tttataatat ttaccgcctg cgtattattg
    44881 acggtaaacc ttatgttctg gaacaaactt atatgagtac cgatgttatt ccaggtatta
    44941 ctgaagatat tttacaaaaa tcgatttaca attacattga aggaaagtta ggattgcata
    45001 ttgccagtgc tacaaaaatc ttacgagctt cttctagttc agaaaatgag caacattact
    45061 tgcagctcct tccaacggaa ccggtatttg aagtagaaca agtggcttat ttggataacg
    45121 gaactccgtt tgagtactcg attagtcgtc atcgctatga tttatttgaa tttaattctt
    45181 ttgcattacg acattcctcc taggagaaaa tgtgaaaatg aagccaatct tttacagact
    45241 ctagtttaag aaaaatttaa aacagggcaa gaaggtccca tctatgctta aatggtttct
    45301 cttttctaaa taagatggct ttaaaagagt gatcgttgta tccatcatgt tgaaaaatat
    45361 cttcgtatag cttatagagt aggtactgaa attgttcacc tgatctactt cttatagtta
    45421 tttagtttta aatagtgttt caaacattct tacactgacg agaagttttt gagtcttttc
    45481 ttgtaacaca tatagtatac tgtggttaga atagtagact gtgacttcta acaaattgct
    45541 agaaatgaat ttcaatctcc caatttattt gttcatatct tcttttaata tattaaataa
    45601 attctaaatc ataatcattt aaaaaaattt tattttttat ttttcattac gaataatata
    45661 gatgaagggg aaagagtatg aaaacagaac tgtttctttt gctattagtt caaaaggaga
    45721 aaaaatgaaa gtagaaaata tttcgtatag ggtggatcat cgtaaattgt ttgataatat
    45781 ttcttttgat acttcgagtt cagacgtgac attaattact ggtaaaaatg gtacaggaaa
    45841 gtcaacttta ctatagtaga ttgaaactag aatagtacac atctacttct aaaatattgt
    45901 tagaaatcga tttgactatc ctgatctatt tgtcctgttc ttatttcatt tcactatatc
    45961 tcaaattgag tatgacgaag tgcgctccca tgtcctggga acgcactttc ttcatatttt
    46021 tcatattctt gaatccatcg ataaagacta ttgggatgaa tttttaaagt tgaactaatc
    46081 atttttacag gatgagattt acagcagaga gtttgaaggc tttatcaaag gtttttcttg
    46141 gcataatgac ttttcctcgt ttccacttaa ttttgtgtct actttattat accaagtcca
    46201 cccttaagtt agataataaa tctaacttaa ggaagctaga aggatgagaa tccaggtggt
    46261 caagagtccc aaacttaagc tgatggggac acccagaata atttgctttt tgaaggcaag
    46321 gccacgttcc tctatattgg gaagtgagag ttgaatgaga gaaccagctg atgaaaaggg
    46381 tgagatatta gtagatagag cgccaataac ggtggctgtt gtgagtaagt gaatatcaat
    46441 ctgaggattt tgagcactga tgatagcaat gatgggaaag agggctggag ctacaacgga
    46501 tagggtggaa ctaaagagtg acatcactcc ggctatcaca caaaagaaca gaggtaacca
    46561 gaaatgagga atggttgttg tcatgaggtg ccctatcagt gtgactaaac ctgacttgac
    46621 cgctagagac attagtaagc tcatgccgca gagcatgata attgtagccc agggaacctt
    46681 agctaaaatg gcttcttgct tccctaattt gagccttaag gcgaggcaga ccatgagtat
    46741 tgagacaaag ccaatatcaa atgttttttg ataagtagct atccaggcga tgtttgggaa
    46801 aatgagatgc aacaagggaa aaagccaaac caaaaccatg ctgctgatca tgagcaaggt
    46861 ggtttgtctt tgaaccttgc tgaggagtgg tggttggtca atagtcaagg atgagtttgt
    46921 tcttccctta ctatagtgac tgtaacagga taataaaagc aagacgatga gtgggtagat
    46981 aatgctgacg ataaagatat gattgccaag tgaaaaagct tgctcttccc atcccatttg
    47041 cttaaacagg ccttgaaaga caatgcctga gctactggtt atcaaattag cccctcctga
    47101 agctccccaa ttgacggctt gagctccaat caaagggtgt ttgtccgctt tttgacagag
    47161 ggtaatcgct agaggacagc aaacggccat agtagtgaaa aatccagcac ctaaagcaga
    47221 caaaagggtt gccatcaggt ataaaatcat gtagagggcg ttagggtggg tgcgtgtgcg
    47281 gtagagaatg tgttgagcca aaacatcaag agtaccgtta gttgttgcaa cgttataaaa
    47341 gagagagacg ctaaaaatgg taaaaaagag tgaggttggc caaaaatgaa gaagttcttt
    47401 ggggcttaat cccatgagag tggttgcgat gaggtaagaa aaagcaatag ccagcaggcc
    47461 aatattgatt ttggtgcggt aaccaattcc aatggctaga gcaatggcgc taatcattat
    47521 taaatgaatc attgaattgt cctttcagtt agaatatgga aataggttgg caattttggc
    47581 gctcaaaaga ccacctgttt cctattggta cttgactatt atacacaaaa aaaatacaat
    47641 tgtcttgaca attgtcttga cacttgttag aattaaaaat gaggtattca ggcttgtgat
    47701 ttcctatgga agttaatagt gattgcctct aatgcttaca agtgatatta aaaatagagg
    47761 acctagtgat gtcaatcatt tcaactgatt taaccccttt tcaaatagat gatacattga
    47821 aagcagcctt gcgagaagat gttcattccg aagattacag taccaatgcc atttttgatc
    47881 atcatggcca agccaaggtg tcgctttttg ccaaggaagc tggtgtttta gcggggctaa
    47941 ccgtttttca aagggttttt accctatttg atgccgaggt gaccttccag aatcctcatc
    48001 aatttaagga tggggatcgt ttgactagtg gcgatttggt tttagaaatc ataggctcgg
    48061 tgagaagtct cttaacatgt gaacgcgttg ccttgaattt tttacaacat ttatcaggga
    48121 ttgcttcgat gacagctgct tatgtagaag ccttaggcga tgattgcatt aaggtatttg
    48181 atactcgaaa aactactcct aatttacgtc tttttgagaa atatgccgtg agagttggcg
    48241 gtggctataa tcatcgcttt aatttatcag atgctatcct gctaaaagac aatcacattg
    48301 cggcagtagg tagtgttcaa aaggcaattg ctcaagcgcg tgcctatgcc ccttttgtga
    48361 aaatggtcga ggtggaagtg gaaagccttg ctgctgccga agaagctgcg gcggcgggtg
    48421 ctgatattat catgttggat aatatgtcat tggaacagat tgaacaggcc attaccctaa
    48481 ttgcaggacg ttctcggatt gaatgttctg gaaatattga tatgaccact attagccgtt
    48541 ttcgtggttt agcgattgat tacgtctcca gtggtagttt aacccatagt gctaagagtc
    48601 ttgatttttc catgaagggg ttaacctacc ttgatgtcta agttgtaaaa taaactaact
    48661 ttttaaagga tgtctttcct ctagaacgag ttttatgtca gatagtttaa acgcctcttc
    48721 aaatatagta aaatgaacca aaaatagtac acaatgtggt ataatctttt tatggcatat
    48781 tcaatagatt ttcgtaaaaa agttctctct tattgtgagc gaacaggtag tataacagaa
    48841 gcatcacacg ttttccaaat ctcacgtaat accatttatg gctggttaaa gctaaaagag
    48901 aaaacaggag agctaaacca ccaagtaaaa ggaataaaac caagaaa
//
LOCUS       DBDESG010000008        46963 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.008, whole genome shotgun sequence.
ACCESSION   DBDESG010000008 DBDESG010000000
VERSION     DBDESG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 46963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.008"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(44..370)
                     /locus_tag="ACKB38_000441"
     CDS             complement(44..370)
                     /locus_tag="ACKB38_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979268.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFRSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDG
                     FDYEEW"
     gene            complement(407..616)
                     /locus_tag="ACKB38_000442"
     CDS             complement(407..616)
                     /locus_tag="ACKB38_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979269.1"
                     /translation="MKQKNYLVSNATVRQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            complement(768..2648)
                     /locus_tag="ACKB38_000443"
     CDS             complement(768..2648)
                     /locus_tag="ACKB38_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HGL7979270.1"
                     /translation="MVRFTGLSLKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDVTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2642..3511)
                     /locus_tag="ACKB38_000444"
     CDS             complement(2642..3511)
                     /locus_tag="ACKB38_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGL7979271.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPGVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3594..6239)
                     /locus_tag="ACKB38_000445"
     CDS             complement(3594..6239)
                     /locus_tag="ACKB38_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGL7979272.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYNLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMCSENVRVPESQHLFLDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6330..7610)
                     /locus_tag="ACKB38_000446"
     CDS             complement(6330..7610)
                     /locus_tag="ACKB38_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGL7979273.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7782..9866
                     /locus_tag="ACKB38_000447"
     CDS             7782..9866
                     /locus_tag="ACKB38_000447"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGL7979274.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAINQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKSSAQVDWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMTPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9906..11585)
                     /locus_tag="ACKB38_000448"
     CDS             complement(9906..11585)
                     /locus_tag="ACKB38_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGL7979275.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQTGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12340..13569
                     /gene="arcA"
                     /locus_tag="ACKB38_000449"
     CDS             12340..13569
                     /gene="arcA"
                     /locus_tag="ACKB38_000449"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGL7979276.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13627..14643
                     /gene="argF"
                     /locus_tag="ACKB38_000450"
     CDS             13627..14643
                     /gene="argF"
                     /locus_tag="ACKB38_000450"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGL7979277.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14808..15755
                     /gene="arcC"
                     /locus_tag="ACKB38_000451"
     CDS             14808..15755
                     /gene="arcC"
                     /locus_tag="ACKB38_000451"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGL7979278.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15964..17475
                     /locus_tag="ACKB38_000452"
     CDS             15964..17475
                     /locus_tag="ACKB38_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGL7979279.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17497..18828
                     /locus_tag="ACKB38_000453"
     CDS             17497..18828
                     /locus_tag="ACKB38_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGL7979280.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18923..19030
                     /locus_tag="ACKB38_000454"
                     /pseudo
     CDS             18923..19030
                     /locus_tag="ACKB38_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(19234..19524)
                     /locus_tag="ACKB38_000455"
                     /pseudo
     CDS             complement(19234..19524)
                     /locus_tag="ACKB38_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
     gene            complement(19512..19763)
                     /locus_tag="ACKB38_000456"
     CDS             complement(19512..19763)
                     /locus_tag="ACKB38_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGL7979281.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            19967..20125
                     /locus_tag="ACKB38_000457"
     CDS             19967..20125
                     /locus_tag="ACKB38_000457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979282.1"
                     /translation="MKIKEQIRKLATGCSKHRFEVADRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            complement(20162..21046)
                     /locus_tag="ACKB38_000458"
     CDS             complement(20162..21046)
                     /locus_tag="ACKB38_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGL7979283.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21191..22342)
                     /locus_tag="ACKB38_000459"
     CDS             complement(21191..22342)
                     /locus_tag="ACKB38_000459"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGL7979284.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22488..24254)
                     /locus_tag="ACKB38_000460"
     CDS             complement(22488..24254)
                     /locus_tag="ACKB38_000460"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGL7979285.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24337..27453)
                     /locus_tag="ACKB38_000461"
     CDS             complement(24337..27453)
                     /locus_tag="ACKB38_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGL7979286.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(27463..29757)
                     /locus_tag="ACKB38_000462"
     CDS             complement(27463..29757)
                     /locus_tag="ACKB38_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGL7979287.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTVFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(29816..30616)
                     /locus_tag="ACKB38_000463"
     CDS             complement(29816..30616)
                     /locus_tag="ACKB38_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGL7979288.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(30613..31386)
                     /locus_tag="ACKB38_000464"
     CDS             complement(30613..31386)
                     /locus_tag="ACKB38_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGL7979289.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(31411..31881)
                     /locus_tag="ACKB38_000465"
     CDS             complement(31411..31881)
                     /locus_tag="ACKB38_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979290.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(31872..32303)
                     /locus_tag="ACKB38_000466"
     CDS             complement(31872..32303)
                     /locus_tag="ACKB38_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7979291.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(32293..32736)
                     /locus_tag="ACKB38_000467"
     CDS             complement(32293..32736)
                     /locus_tag="ACKB38_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGL7979292.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENVQ"
     gene            complement(32748..33386)
                     /locus_tag="ACKB38_000468"
     CDS             complement(32748..33386)
                     /locus_tag="ACKB38_000468"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGL7979293.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(33501..34904)
                     /locus_tag="ACKB38_000469"
     CDS             complement(33501..34904)
                     /locus_tag="ACKB38_000469"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGL7979294.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            35080..35853
                     /locus_tag="ACKB38_000470"
     CDS             35080..35853
                     /locus_tag="ACKB38_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGL7979295.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(37013..38518)
                     /gene="adcA"
                     /locus_tag="ACKB38_000471"
     CDS             complement(37013..38518)
                     /gene="adcA"
                     /locus_tag="ACKB38_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGL7979296.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYHTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(38528..39334)
                     /locus_tag="ACKB38_000472"
     CDS             complement(38528..39334)
                     /locus_tag="ACKB38_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGL7979297.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(39327..40031)
                     /locus_tag="ACKB38_000473"
     CDS             complement(39327..40031)
                     /locus_tag="ACKB38_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGL7979298.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(40031..40471)
                     /gene="adcR"
                     /locus_tag="ACKB38_000474"
     CDS             complement(40031..40471)
                     /gene="adcR"
                     /locus_tag="ACKB38_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGL7979299.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(40626..41894)
                     /gene="dltD"
                     /locus_tag="ACKB38_000475"
     CDS             complement(40626..41894)
                     /gene="dltD"
                     /locus_tag="ACKB38_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGL7979300.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMNYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(41887..42126)
                     /gene="dltC"
                     /locus_tag="ACKB38_000476"
     CDS             complement(41887..42126)
                     /gene="dltC"
                     /locus_tag="ACKB38_000476"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGL7979301.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(42140..43384)
                     /gene="dltB"
                     /locus_tag="ACKB38_000477"
     CDS             complement(42140..43384)
                     /gene="dltB"
                     /locus_tag="ACKB38_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGL7979302.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(43381..44931)
                     /gene="dltA"
                     /locus_tag="ACKB38_000478"
     CDS             complement(43381..44931)
                     /gene="dltA"
                     /locus_tag="ACKB38_000478"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGL7979303.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(44952..45083)
                     /locus_tag="ACKB38_000479"
     CDS             complement(44952..45083)
                     /locus_tag="ACKB38_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGL7979304.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(45397..46575)
                     /locus_tag="ACKB38_000480"
     CDS             complement(45397..46575)
                     /locus_tag="ACKB38_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGL7979305.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMEIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
BASE COUNT        14003 a        10241 c         8475 g        14244 t
ORIGIN      
        1 gaagtctatt gaaatactaa gcctacacag actgatttca attttaccat tcttcatagt
       61 caaaaccatc ataatctgga tattgttctg gcgacgttcc aaccgaacgt acgagataag
      121 cttcttcgtc ctctgtctcg atttctctca gcacttggca ttcaaaacgc cattcatcac
      181 cgaagtcgaa aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt
      241 ctgtgtaacg ttcttcaaca tcgtcactaa caaagctacg aaagtaagaa tctgcatgac
      301 tccactcaac attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcaaaactcc
      361 ataaaatcac atctgctagt tccgctaacg tttttgtttt tggaatctat atatgtcggt
      421 agcaaccttt tttaaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag
      481 cttctatttc ttgttttact aattttaagt gtagcataat acctccaatt gctcttagga
      541 aacattcact ttctgctatt ttatcataag tttgtctaac tgtagcattt gaaaccagat
      601 aattcttctg tttcacgcga aacaccctgc gccaagcaag gtgttcttgt ttttatactc
      661 aatgaaaatc aaagagcaaa ctaggaagct agctgcaggt tgctcaaaac actgttttga
      721 ggttgcagat ggaagctgac gtggtttgaa gagattttcg aagaatatta agtcgtataa
      781 atcgtcgacg ctgtcgcaat ggtatgccac tggttggctg tagttgctgc gaaatccttg
      841 tctgcgtaga agtcagtaaa tggtaggatt tcaacttcca gctcgtcgat gcggtcaagc
      901 tgaccagcca gataaacctc gatacggctt tctgctcgtt tgatgcgttg caagagtccg
      961 cccatacgga tgtcaactgt atccaaacca aagaccttgt tttctttcag ccattggtgg
     1021 ctaaagaggg catggaagtc ttcaatttgg cttctaagtt ctggtaattc ttgtctggcg
     1081 atttgttgta aactttcttt atcatccgct tggtaggcct gacgaatgcg tcgtcccaca
     1141 tctactttgc tacttaaaat agcattcaac tgggcctgag tttcaaagag ataggcatag
     1201 tttccagctt tttctttaat gttagcaagc gtctcagcag cctgagcgaa gtgcggtttg
     1261 tcctgttcag gtgtcatgtg ttgatcaaga atcggacaaa gaatatcctg ataaaaaaca
     1321 tagcggttgg gattgatacc gctgagattg cctggtaggt ctggtaagag gttggcaagg
     1381 tcaatctgca taaaatcctc aaccgttaga ccagtattgg tcttgaagtg cgcagacaaa
     1441 ccatctaggt cattgcgata gctgagttct gcccagattt gcaagcttgg taggatagag
     1501 aactgggcag tttcaccacc attgtctccc caacccgtta cgatgacttc tttaatctga
     1561 ttggcacggc aggctttatt agcctcgata gccactagac ggctaaaatg gttgtgaggt
     1621 gtaaagccaa tccacttcca agctccccct gcaaatgcaa ggtcatggct aatcttgtga
     1681 tgattgcgga aattacggtt gtatttttcc tcgctatcct gataataatc ccagtaaacc
     1741 agagtcacac ggtctttgag acggtctagg tagacacgag tttcctctgg aatttccaca
     1801 tcacggtcgt actggccatc cgctgacatg agtttgaaga acatatcact ccacatctgg
     1861 cagtggaaac catatttgtc agcaatatcc agcacgcgct ccaagtgttg gcacatgagg
     1921 agactacgat ccacaacacc gttcagaatc aggtagcgtc ccaaaccaac caagtgggct
     1981 tcgtccatcc cgatattgac cttgcgagtc ttcagtttag acaacgtggc aaacatgcca
     2041 tcaatcaagt cataaacctt ttcttcgcca atgagaagaa tgtcctctac atcacggagc
     2101 tcctgcactt ccttgacacc ccatttgaca aaggccgaca agtgggccaa ggtctggatg
     2161 catggtacaa aggtcacgtc aaactgttgg gcataggctt cgatttcctg caactcctct
     2221 gctgaataag ctccacggaa atagccaaag taaggctgcc cttcaatctg gtaagtgtct
     2281 tccatgtaaa gctcaaaggt tgagtagccc atgagagcca atatctcaat catctgcttg
     2341 gcagaagcca cattcagcac cgcatttcga gaacagtcaa ccatgtaagc caaatcttcg
     2401 taagctgctt gttcctcaat ctctacttta tcagcttctg ctagaactgt taccaacaag
     2461 gacaaggcac gataaagttg gtgaggtttg cggtaggtca attgatagtg accttcctca
     2521 ccctcgatag agatagatgc ttggtcagac tgagtgacag ccacttccac atctggtaga
     2581 gaaatgtgac cttttaaaac ctcaatagct tgcgtttgtt tgagactaag tcctgtaaat
     2641 cttaccattg cttttcctcc tgtagccagt tgacaagagc accgtagaga ttggcatctg
     2701 cgtgataggt gcaggcctgg ataactggtg cgaccgtgta ttcttcgtag gcatcgacaa
     2761 agtcttcaac agccttcttg acaccttgga taaaatctgg attttgactg atagagccac
     2821 ccagactgat gacacctgga tcgatcagat actggatatt gagcaagcct tgcgccagat
     2881 tgcggttcat gcgctcaatg gcttcttgac aaaggatatt accagctgcg gcctcttggt
     2941 aaatcttgcg accgtcccaa tcagtatgac cagatttttc aatcacgtat cgtaccatat
     3001 tcccagttga tgctagttgc gaccagttat taagtttttc agcaggggca agggttgtca
     3061 tgtagccaaa ttctccaccc agaccgtggc gacctcgatg aagtctacca ttgataatca
     3121 tggctccgcc aatccctgtc ccaatcacga cacaggctgc attttcaagc tctggatgag
     3181 ctagtagttc actgagtcca acgcagttgg catcattttc taaatggaca ggtagctgat
     3241 aagagctaag cgcctcatac caagaaaagc catggatgta gggcaccgca ctgaagccat
     3301 caatcacacc tgtctcttga ttgactgcac ctggaacgct catagcaatc ccactgtaat
     3361 cctgttctga caagcgttga tctagccacg ctagtaaatc ctccaagttt tcaggcgttg
     3421 aaatacttgt cttatccagt attttcccat caggagtcag actggcaaac ttaatcccag
     3481 tccctccgat atcaatcgtt gcaatggtca ttgtttttac cataaaaggg gcgaatcagc
     3541 agttagttct tactttttcg cccctccttt cttttgatgt ttactttttg aaattaaatt
     3601 tcttcttttt tgatgaattc tgtacgaatc tcttgtggag ccaatagtcc tgaatgaact
     3661 gggtatggtc gttcaagtag gtcaaggaag agatgttgac tttctggcac acgtacattt
     3721 tcactacaca tattgtagta acgaagcaca tagccttctt cattttcagc taccttaaag
     3781 gctgttggac aaacttgcgg tatgctgaga acagaatggc tcaagaggct accagtcgca
     3841 accacgcttc cttcctgtct agcaagctga aggctggtaa acggtgtctg caaggctttg
     3901 gcacgacgat aggctgagaa gcgttcttgg gcttggtggc attcaagtgc aaactcgact
     3961 tcaaactccc gcaagcattg tgcttctggc gttgggaagt agccccagtc acctagctca
     4021 cctgatgcac gcaaaatggt cacggcaatg gtgttatccc caaggatttc gtattcattc
     4081 aatcccttgt tggatacagt cacccctttt tcatcgtcat acagactgac aaaagcttgt
     4141 tggtgttgag gattttcagg gttttcccat gaagcagctg gtttgtttgg tcgtgtcacc
     4201 acctcataga tactttcaga atcattgctt ggacgcgtgt tatgagtctt gaccaagaga
     4261 cggatacggt gatccttggc agtgttagta aagcgagtct tgaagcggat ttgtggattg
     4321 tcaacgaaga cagtcaactc agtttccaga ggaatgtttg tcaattcttc tgaccgtcca
     4381 gcctcacgct tcataaactc gatgatacct tgttgctctt cttctagctt ttcatccgca
     4441 ctgacaggca cggtcaattc atgtttgagc aagattttag catagcaagc tgtgttttcc
     4501 aagacctcgt ggcccttaag ctctgcaaag attggctctg ttccttttgg ttggaaatag
     4561 atatactcgt ttccgatgtc cccacggtct tcaaagcgga taaagtcttc ataggcttcg
     4621 tgagttgtct tgtcatagac tgtgatgttg tcatccacac tcaccgttac gaatggtgta
     4681 tcaatcactc cgttttggta aataccctca cggtgttctt gttttccttc cagcaattgg
     4741 aaggttgtcc aagaaagcgg cgctaggtga actggaatgg tcacgcgcac ttgacgagca
     4801 atacgagctt ggcggaactt gtcttttggt aaattatact caaaattagc tccgaggtct
     4861 tcgattgtag cctctacagg acgaccatcc aagtcctcca cacggtaact tggcaagata
     4921 agagcagcca tctttttgta gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc
     4981 acatcaatca ctgtgctgac agtatcgacc ttatcatgca agcctgtgtt aatgacagta
     5041 aagagatagt cactttgagc cttatccgta gcaattttac ccttccactc gttgagcaag
     5101 ttacttttaa caaagtttcc tacttggttg accttggcaa aacgcgtttc catctcgcgg
     5161 tgaacttcgt ccacgctaca gccacagata ctatcatgtg gcgcattctg caaaagtgtt
     5221 ttccaagcat aggtcaactg gtccttgtgg ttgtgtccac cagtgataat agtcaagggt
     5281 tctaccactt gctctaggag gttgctattt tcttggaagg cttgttttag gtaaatgcgg
     5341 gatgaagaag tgttggcaag tgtgtaccag ccatctgttt cctgactggt caactcgcct
     5401 gtaacagttg ataagtgttc aggaagcgca ccttctacag cttgaacata ttcatcaaaa
     5461 gaactatgaa caaagattac atccgggaag agttcatttg ccacacgaat ggcttcgctc
     5521 agatttttct gtacaggctg gtggtcacag ccgttcatca tcaaccattg gttggtcgaa
     5581 gcgtaggcac gcacatctga caatttttgt ttccagaagg tcaaggcctc atctttgtca
     5641 actggaattt cattcccgtt actgtaccag ttggcaaaga gaatacctaa aacacgacta
     5701 ccatccacac cctgccagta catttctgaa aactgagacg taaactgctc atcttcaagg
     5761 acttggttgt caaatccaat cggcttcaca ccacgaccaa aggccgccac gtgaatgcct
     5821 gatttttgaa gaatttgagg cgcttgtccc atatttccaa aggtatctgg aaagtagcca
     5881 atctgggttg atttacccca tttggcagct tcttgttgac caatcaaggt attgcggaca
     5941 ttggcttcac tggagatcaa gtagtcatcc tgcaagatgt aaaagggacc aattttaagt
     6001 ttgccctcgt caatgtagcg ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag
     6061 tcatcaagga caatagtttg tccatccaag tggaaactct tgaactcagg gtcattttca
     6121 aagagatcaa agagattgtc aaacaattcc accaactgca tacgatggct ttcaaaaggc
     6181 aagtaccact cacgatccca gtgactatgt gagataatat gtacaacaac attttccatg
     6241 aagtaaaacc tcattctaaa tttaaatttt aacgttttaa atgcaaactt gtgattctga
     6301 taagaatcgg ttgagctaaa gcgagcccct tagcgaatat ccaagtaatc caagaccaac
     6361 tcacagaaca tcatgttagc ccaggagaac cattcacgag agtagagggt cggatcatct
     6421 acatgaaagc tttcgtgcat gacacctgta ccaccatcgc aggcaaccag ctgatccagc
     6481 aagaattttt tctctgcctt atctcttgtt gtcaagcctt ggatagaaag ggcgattggc
     6541 cagatatagc gatagaaggt atgagaactg ccgagaccgc ttgcgtattc tccttggtag
     6601 aagtatggat tttcagagct caaaatggta cgacgagtag cttgatacac ttcatcatcg
     6661 accgaacagt agcccagata gggcgcagct agtagacttg gtacatttgg atcatccatg
     6721 atgctggcat ttcctaggcc atccacttca aaagcgtaaa tcttttcgcc cttgctgttg
     6781 gtggtgtaag cgtagttttt gattccttct tggatttcat cctgaagacg cttggcatca
     6841 gcaataacac tctggctatc agctaggttt aatgctgcga agatttcttg cacataaccc
     6901 aagactacta cagcaaacat atttgacgga atcaagtaac tatactggca acagtcatca
     6961 ctcggacgaa aagctgacca agtcatacct gtcactgcaa agtcaggtcc aaagccatca
     7021 tttaccaagg tgtcttcctt acggtccgta tctcggacaa aacgataagg agagttcttg
     7081 tggtcttgtt ccaccgtcca cagatggaga atttccttag tcgctgcgac aaaaatctca
     7141 tcaaactgac tagtctcgcc agtctctttc cagaggagat aagccaactg caaaggatag
     7201 caaagcgaat ccacctcata cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca
     7261 gtctcgtggt gccctttcca gttctcctca atgttgaagg agttagcata gggatccttg
     7321 agtaccaagg tcatctgacg tttgaccaaa cctgcaatgg tctgacgcag gagggcatct
     7381 cttttagcta catgaaggta gggtctgagt tgggctgtcg aatctcgaag ccacatggca
     7441 ggaatatccc cagtcaagac aaaagttgaa ccatcttcta agatttcaac cgtattgtcc
     7501 aaggtatcgg tgtagcaacg ctcgaaaaca tccacccact ctggatagtc cttagcccgc
     7561 tctgctactt catctagcca ctctctgaca atttctttcg aataaaccat tttaaatttt
     7621 acctctttca aacaagtttc atgttcagta tatagctttt gagacagaaa atacatacac
     7681 aaatgatagt catttttcta caaaatagtt atttttgaaa ctccttttct aaaggccctc
     7741 tttttctgat ataatgtaga aaaacagcta aaggaaagac tatgaaacca ctacttgaaa
     7801 ccatcgatac ccgctttgga actaccaaca agcatgcctt ttctcgggga aataccctgc
     7861 catacacagg cgtgcctttt gggatgaatt actttgtgcc ccagaccagt gaccaggatg
     7921 gatcttggtt ttttgatcca catctgccta tctttcaggg gattcgatta actcaccagc
     7981 ccagtccttg gattggggac tactcttggc tccttctgac acctgtcacc agtcaactgg
     8041 ggggagacag cctctttcac cgccagtctt cttatgatat agataaagct tgcttccagc
     8101 ctcactattt gaagctcttt tctctgcgtt atcagattga aacccagctc acaccgactt
     8161 gctatggtgc ttctattcgt ttgaatcaaa agcaaggcaa agccctctcc ctctatcttc
     8221 acgcagcaga tgaactgaca gtagagcaag tagataagcg gactcttgcc ctgcgacaag
     8281 aaggtaaaac tgaaactaac aaaaattcgc taacgatgtt cactgccctg caaatgaaca
     8341 cggatattct tgctatcaac caagaagctg gagactggcg aattgactta gcaagtagtc
     8401 aaaccgagat gcaactagcc acttctttca tctctccttc tcaagctcta atcaatctac
     8461 ctcaagaaga ttttgatagc tgtaaatcaa gtgcccaagt ggattgggaa aatctcctcc
     8521 atcgttttga cattatagag acaggagagg ctgaccgaac cttctttgac cactgcctct
     8581 acagactctt cctcttccca cagacttttt atgagattaa tgaatcaggg caagccatcc
     8641 acatggatct ggctactggt actgtcaagc ctggtgtcct ctttagcaac aatggtttct
     8701 gggatacctt ccgcaccacc ttccccctct ttgcccttat cataccggag cactatcaac
     8761 gctttttaga aggtttcctc aatagctacc gcgatactgg tttccttcca aaatggctgg
     8821 ctccagatga acggggtatg atgccaggta cacttttaga cggtattatc gcagatagcg
     8881 cctgcaagga catgaccccc gacctagaag gagaactctt ccaagccatg ctcgaaacag
     8941 ccagcaaagc cgaccctctc ggcatcaatg gccgccacgg actagcccaa taccaagaac
     9001 taggttacct ctctaccgac caccacgaaa gtgttagcca tactctagac tatgcctata
     9061 gtgacttttg tatcgccagc tgtgccaaaa aactagagaa catagaaatc gctgaaacct
     9121 acaaggctgc ttcacaaaat taccgccagc tatttgacgc tgagacaggt tacatgcgag
     9181 cacgagacaa tcaaggaaac tttcaccctg acttctctcc ttatagttgg gggcgagact
     9241 acgctgaatg ctctgccatt caagctactt taggcgttct ccacgacatc cctggcttaa
     9301 tccaactgat gggtggaaaa gaaaccttta gcaactatct tttgaaagcc tgtcaagatg
     9361 ctcccctctt tgaaacaaca ggctatggtt acgaaattca cgaaatgagc gagatggcta
     9421 ctgctccttt tggacaaata gccatttcca accagcctag tttccacatt ccttatctct
     9481 tccgttacag cgattaccct gactacactg cccttcttat caagacgctc cgtcagaaag
     9541 cttttcaccc aagttgggaa gcctatcctg gagatgaaga caatggtagt ctctctgctt
     9601 ggtacatctg gtcagctctc ggattttatc caacctgtcc aggaaaacca agctatgacc
     9661 tcggaatccc tctctttgac catctacgag tctacctcgc taaagaagat aaatggctgg
     9721 atatccatac taaacaaaac cacaaccatt ttaactttgt caaagaatgc cgactggaca
     9781 aaacactcgt atcaactatt caacaccaag acctcttaaa agctgaacaa ctaactttca
     9841 ccctcagctg gttaccaagt cactaataac caaaagaacc tttgcatcgc gcaaaggttc
     9901 ttcttttatg aaacttggac ttgaagttga tccacaagct gaccatccac cgttaggttc
     9961 agatagaaat ccaaggtttt atctcctgcg aaatacaagg ttggtagcgt cagacttttt
    10021 tcagtctcac cagtttgaaa cgcaaggact tgaatctcat cctgataggc gacaccatgg
    10081 acacctgtcc ccggttgaat cgaaatctta gcttctaaag gaccactaca ttctctgcgc
    10141 ttaactgtaa aataggcatt ttctcccttt gccacagcta ggcttttttc tgcaaatgct
    10201 agttcctgaa caacttcttt ttttgataat ccaggagttt tataaaggga aatcttggtc
    10261 aacaaaggca aagcctgtga ttctgtaatg actacacgta tcttctgtgc ctcaacaact
    10321 gctcctcgta agagacgttt gtaaccaaca gtatgacccg aaccaaactc ctgccagaca
    10381 ccatccacct ctacttgcac atgaaaagca gcgattcgtt gccctagctt caaatcttct
    10441 cttaactcaa ttacatcaaa agttttagga gaacctaagt cgagttctaa ctggatgggc
    10501 aagtctgcat cgcttgccca agagctggtc tcaaggccgt ctgtcaaatg gcgacaagca
    10561 aagtctgcgg aaagagctgg accagatacc tcagctccca gagccaaatc ttctttatag
    10621 agctcattgc gataggtcgc aaattcataa agtcgttcaa tatcctttgc atcaaagagc
    10681 ccagcttgat tcggcggaat attaagcaag agtggagttc ctcgccctac tgagtgaaag
    10741 tagatttcga ccaactcctc gagagactta ggatcctgat cctcatggta gaaccagcct
    10801 ggacggatgg aaacatctgc ctctccgatt gaaaaaatcg tgcccgaggg atccccgtgc
    10861 tgaagatagt tcagctctgc ttctgttcct agtttatcag gattcacctt ttgccacagt
    10921 ggatcacctg cataccctcg ttcattgcca atccagcgga tactggtgcc ttctgttgaa
    10981 aaaatcaagc aatcgccctg caggtcacga atggtttcaa accatttttc aaattcataa
    11041 ttaacctttt gcgcgccctc tcctctggca ccatccatcc aaacctcagc gaacttacca
    11101 gcattcccat agttaggatt tgataagatt tccttcaact gagccagata ataggcattg
    11161 tagtccgctt ctcggtccac atgatagagg ggactatggg catcccacgg tgacaggtag
    11221 acccccatat ccatatcaaa ctctgtggca gcttgggata cttcaaggag caagtcgccc
    11281 tttcctctcc tccaaggact gaccttaacc gaataatctg tgtgagctgt cggataaagg
    11341 acaaagccat cgtggtgctt gaccaccaaa atcaactttt tgaagcccgt ttccttgagc
    11401 acacgaaccc actcacgcgc atccaactga ctcgggttaa agcgctcagg atcctcctgt
    11461 ccagtccccc attcttggtc ataaaaggta ttaggaccga agtggataaa agctgctagt
    11521 tcatctccca gataagctag ctgagtctga cttggtaacg gtccatgcgg tttgattttc
    11581 ttcatcgtta tatatctcct tctttagcaa cttctcttat tcacaaagtt atccacaggt
    11641 tgtggataat aaaccatgat ctaacagtca gctctttcca actccaactg gtaggctcag
    11701 gatttttctc ctcaatattc cctcttcaaa cagctctgtc atatggttct gctaacacct
    11761 ctcagaatgc tactctccat aattattctc aggcttcttt caggtcattg tgtgaagacc
    11821 attcactatc cagacttttc cacagattgt gaataagtga ttattctaaa ctatcacctc
    11881 gtcgactata acaaaaaata aaaagagggt caataagata attatctctt cctaaatact
    11941 cagaaaagat tctataattt gtacaattga acaagaaatg cttctccaaa atctatcttc
    12001 aaaagaatgt tgcatctttt tatcctttca cacaaacatt tctctttata cagatccaag
    12061 tgataaccag atgataattg gacacttttt gatcatttgt atattttttg aaaaattttt
    12121 taaaaaaatt ttatatcctt tttatagaag tttatgcatt tgtatctatg tattctatcc
    12181 tatgttatgt ataataacat aaaattgtgc taaacatcat aatttatatt attttccgag
    12241 aaattatagc cattgacatt acctaggaat tgcgttataa catacatgta agcggtaccc
    12301 aaaatggtgc caagtcaaaa tttttaagga ggaaaataca tgtcttcaca tccaattcag
    12361 gtcttctcag aaattgggaa actgaaaaaa gttatgttgc accgtccagg caaggagtta
    12421 gaaaacttgt tgccggacta tcttgaaagg cttctttttg atgatattcc tttcttggaa
    12481 gatgctcaaa aagaacatga tgcatttgcc caagctcttc gcgatgaagg aattgaggtt
    12541 ctctacctag aacaactcgc tgctgaatca ttgacctctc cagaaatccg cgatcaattt
    12601 atcgaggaat acttagacga agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt
    12661 gaattgcttc acggcatcaa ggacaaccaa gaattggttg aaaaaacaat ggctgggatt
    12721 caaaaagttg aattgccaga aattcctgac gaagctaaag atctaactga cttagttgaa
    12781 tcagagtatc catttgcaat tgacccgatg ccaaacctct atttcactcg cgacccattt
    12841 gcaacaattg gaaacgccgt atcgcttaac cacatgtttg cagacactcg taaccgtgaa
    12901 acactctacg gtaagtatat cttcaaatac cacccaatct atggcggaaa agtggatttg
    12961 gtctacaacc gtgaagaaga tacgcgtatc gaaggtggag acgagttagt tctttctaaa
    13021 gacgtccttg cagtaggtat ctctcaacgt acagacgcag cttctatcga aaaacttttg
    13081 gtcaacatct tcaagaaaaa tgttggcttc aagaaagttt tggcctttga atttgctaac
    13141 aaccgtaaat tcatgcactt ggatactgtc ttcactatgg tagactatga caagttcact
    13201 attcacccag aaatcgaagg cgaccttcac gtttactcag ttacttacga aaacgaaaaa
    13261 cttaaaatcg ttgaagagaa aggtgactta gctgaacttc ttgctcaaaa ccttggtgta
    13321 gaaaaagttc atttgattcg ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa
    13381 tggaacgacg gttctaacac tttgaccatc gcacctggtg tggtagttgt ttatgaccgc
    13441 aataccgtga ccaataagat tttggaagaa tacgggcttc gcttgattaa gattcgcgga
    13501 agtgaattgg ttcggggccg tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa
    13561 gaagtgtaat cgctgttcga tattcgtcaa tagaaaatgt aaaaaataga aagaggaaat
    13621 aataaaatga caaattcagt attccaagga cgcagcttct tagcagaaaa agactttacc
    13681 cgtgcagagt tagaatacct tattggtctt tcagctcact tgaaagattt gaaaaaacgc
    13741 aatattcaac accactacct tgctggcaag aatatcgctc tcctatttga aaaaacatct
    13801 actcgtactc gtgcagcctt tacaactgcg gctatcgacc ttggtgctca cccagaatac
    13861 ctcggagcaa atgatattca gttgggtaaa aaagaatcta ctgaagatac tgctaaagta
    13921 ttgggacgta tgtttgacgg gattgaattc cgcggattca gccaacgtat ggttgaagaa
    13981 ttggcagaat tctcaggcgt tccagtatgg aacggtctaa ctgacgaatg gcacccaact
    14041 caaatgctcg ctgactactt gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca
    14101 ttggtatact gtggtgatgg acgtaacaac gttgccaaca gcttgctcgt aacaggtgct
    14161 atccttggtg tcaatgttca catcttctca ccaaaagaac tcttcccaga aaaagaaatc
    14221 gttgaattgg cagaaggatt tgctaaagaa agtggcgcac atgttctcat cactgaagat
    14281 gctgatgaag cagttaaaga tgcagacgtt ctttacacag acgtttgggt atcaatgggt
    14341 gaagaagaca aattcgcaga acgtgtagct cttcttaaac cttaccaagt caatatggac
    14401 ttagttaaaa aagcaggcaa tgaaaacttg atcttcctac actgcttgcc agcattccac
    14461 gatactcaca ctgtttatgg taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa
    14521 gtaacagacg aagtcttccg cagcaagtac gctcgccact tcgatcaagc agaaaaccgt
    14581 atgcacacta tcaaagctgt tatggctgct acacttggta acctttatat tcctaaagta
    14641 taattttaga taataaaccg tctaccaaca gctatgaggg ctgcgactaa tagctttagt
    14701 ccggtcctct tttatgtaat ggtaatctat tatttcttat aaaatatgtg aaaaatcatt
    14761 aaattgaaat ctaaacgcat tctattgagt gtgataaagg agaatttatg gcaaatcgta
    14821 aaattgtagt agctttggga ggaaatgcga ttctttcttc tgacccatca gcaaaggctc
    14881 aacaagaagc tttagttgaa acagctaagc atcttgtaaa attgattaaa aatggagatg
    14941 atctgattat cactcacggt aatggacctc aagttgggaa tctcttgctc caacatttgg
    15001 catcagactc tgaaaagaac cctgccttcc cactcgactc acttgtcgct atgacagaag
    15061 gtagcatcgg tttctggttg aaaaatgctt tgcaaaatgc tctcttggat gaaggcatcg
    15121 aaaaaaatgt tgcctctgtt gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg
    15181 ttaacttgag taaaccaatc ggtcctttct attcagaaga agaagcaaaa gcagaagccg
    15241 aaaaaagcgg agcgactttc aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct
    15301 caccaaaacc tgttgacatc aaagaaattg aaaccatccg tactctttta aataatggtc
    15361 aagtcgtcgt agctgcaggt ggtggcggta ttcccgtcgt caaagaaaac aatggacatt
    15421 tgactggtgt cgaagcggtt attgataaag acttcgcttc ccaacgtttg gcagaattgg
    15481 ttgatgcaga cctcttcatc gttttgacag gtgtagatta tgtatttgtt aactacaaca
    15541 agccaaacca ggaaaaattg gaacatgtga atgttgccca gctggaagaa tatatcaaac
    15601 aagatcagtt tgcaccaggt agcatgcttc caaaagtaga agcagctatc gcttttgtca
    15661 atggtcgtcc agaaggaaaa gcagttatta cttcccttga aaatctaggc gccttgattg
    15721 aatctgaaag cggaacaatt attgaaaaag gataagttgt tttactaata agatgtattc
    15781 tatttctagt atctttatat caaattagaa attattcttg aaaacatgta caatatttca
    15841 aaagatacta gttttagact ttaatatggt aaaacaaata taaatagaaa gcgttttctt
    15901 gaatgtttat ttaagaaagt agttggtttt ttacactttg ttagacatca ggaggaaaaa
    15961 caaatgagtg aaaaagctaa aaaagggttt aagatgcctt catcttacac cgtattattg
    16021 ataatcattg ctattatggc agtgctaact tggtttatcc ctgcgggggc ctttatagaa
    16081 ggtatttacg agactcagcc tcaaaatcca caagggattt gggatgtcct catggcaccg
    16141 attcgggcta tgctaggtac tcatccagag gaaggttcgc tcattaaaga aacgagcgca
    16201 gcgattgatg tagccttctt catccttatg gttggtggtt tccttggcat tgtcaacaaa
    16261 actggtgctc ttgacgtagg gattgcctct atcgtgaaga agtataaggg ccgcgaaaaa
    16321 atgttaattt tggtactgat gcctttgttt gccctcggtg gtacaactta tggtatgggt
    16381 gaagaaacaa tggccttcta tccactcctt gtgccagtta tgatggccgt tggttttgat
    16441 agcctgactg gtgttgcaat tattttgctc ggttctcaaa tcggctgttt ggcatctact
    16501 ctgaatccat ttgcgacagg tattgcttca gcgactgcgg gagttggtac aggggacggt
    16561 atcgtacttc gtctgatctt ctgggttacc ttgactgctc ttagtacttg gtttgtttac
    16621 cgttatgcgg ataagattca aaaagatccg actaagtcac tggtttatag tactcgcaaa
    16681 gaagatttga aacactttaa cgtagaagaa tcttcatctg tagaatctac acttagcagc
    16741 aaacaaaaat cagttctctt cttatttgtg ttgacattca tcttgatggt attgagcttc
    16801 attccatgga cagaccttgg cgttaccatt tttgatgact ttaatacttg gttgactggt
    16861 cttccagtta ttggtaatat tgtcggttca tctacttctg cactaggtac ttggtacttc
    16921 ccagaaggcg caatgctctt tgcctttatg ggtatcctga ttggtgttat ttatggtctt
    16981 aaagaagata agattatctc ttccttcatg aatggtgctg ctgacttgct cagtgttgcc
    17041 ttgatcgtag cgattgctcg tggtattcaa gttatcatga acgacggtat gattaccgat
    17101 acaatcctca actggggtaa agaaggcttg agcggtctat cttcacaagt ctttatcgtt
    17161 gtaacttata tcttctatct acctatgtca ttcttgatcc catcttcatc tggtcttgcc
    17221 agcgcaacta tgggtatcat ggctccactt ggagaatttg taaatgtccg tcctagcttg
    17281 attatcactg cttaccaatc tgcttcaggt gtcttgaact tgattgcacc aacatctggt
    17341 attgtgatgg gagctcttgc acttggacgt atcaacattg gtacttggtg gaaattcatg
    17401 ggcaaactcg tagtcgctat tattgtagtg accatcgccc ttcttctcct tggaaccttc
    17461 cttccattcc tataaaatag tgagtgaggt gattccatga aaatagatat aacaaatcaa
    17521 gttaaagatg aatttcttat atcattaaaa accttgattt cctatccttc agtactcaat
    17581 gaaggagaaa atggaacacc ttttggacaa gcaatccaag atgtcctaga aaaaacttta
    17641 gagatttgtc gagacatagg tttcactacc tatcttgacc ctaaaggtta ttacggatat
    17701 gcagaaatcg gtcagggagc agagcttctg gccattctct gtcatttgga tgttgttcca
    17761 tcaggtgatg aagcagattg gcagacaccg ccatttgaag caactatcaa agacggctgg
    17821 gtattcggac gtggtgtcca agatgataaa ggcccttcgc tcgcagctct ctatgcagta
    17881 aaaagcttgc tggaccaagg tattcagttc aaaaagcgcg tacgctttat ctttggtacc
    17941 gatgaggaaa ccctctggcg ctgcatggca cgctacaata ccatcgaaga acaggccagt
    18001 atgggctttg cacctgactc atcttttcct ctgacctatg ctgaaaaagg gcttctacag
    18061 gtcaaacttc atggccctgg atcggatcaa ctagagcttg aagtaggagg cgcctttaac
    18121 gttgtaccag acaaggccaa ctaccaaggt ctcctctatg aacaggtttg taacggtctc
    18181 aaagaagctg gttatgatta ccaaaccact gaacaaaccg taacggttct cggagtgcca
    18241 aagcatgcta aggatgctag tcaaggtatc aatgctgtca tccgactagc taccattctt
    18301 gctcctctcc aagaacaccc tgctctcagt tttcttgcaa cacaagcagg tcaagacggc
    18361 acaggaagac aaatctttgg tgatatagca gatgaacctt ctggtcacct atcctttaat
    18421 gtcgcaggtc tcatgatcaa tcatgaacgt tctgaaatcc gtattgacat tcggactcct
    18481 gtcttagctg acaaggaaga actagtagag ttgcttacaa gatgtgcaca aaactaccaa
    18541 ctccgctacg aagagtttga ctatctagcg cctctatacg tcgcagaaga cagtaaactc
    18601 gttagcacac tgatgcaaat ctaccaagaa aagactggcg ataacagtcc tgctatttca
    18661 tccggtggtg ccacttttgc tcgcaccatg ccaaattgtg tagccttcgg cgccttattc
    18721 ccaggagcga agcagacaga acatcaggca aatgaatgtg ccgttctaga agatttgtac
    18781 cgtgctatgg atatttatgc cgaagccgtc tatcgacttg caacttaatc aggcaactgt
    18841 ttctaccaaa aaaaatcgac cgattaatga actgcacccc aaaagttaga cagaataaat
    18901 ctaacttttg gggtgtttta ttatgaaatt gagttatgaa gataaagttc agatctatga
    18961 actaagaaag caaggacaaa gcttcaaaca gctttcaaaa agatttggtg tggatgtttc
    19021 tggtctaaag tcatctgaat ctttgagatg agctttataa atcgcttttt tcagtttttg
    19081 cactggtgtt tcgataaact caaacttttt agccgtggta ttgcctgatt ttatagtata
    19141 ttgaaactag aatagtacac ctctccttct aaaacatttt tagaaatcga tttgactgtc
    19201 ctgatcgatt tgtcctgttc ttatttcatt ttactatatt tgagccactt cgtctttaac
    19261 ggctttattc ataagctctt gtaatttttc tttactatca attacttctg attttccgtt
    19321 gtaatttatt gtaataggtt ttaacttacc taatttctcg acacgctcat taatttgatc
    19381 ttttttgaag gctgcttatg tttttcctaa gattttttca aaaatatatt tatcagatag
    19441 cggtttgtct tcttcttcag cttggttttt gtattaattt gaaacataag gaacaaatcc
    19501 ttcatagtaa cctaatgctc ccataagttc aaaagcttgt tttctaattc aaaccattgc
    19561 aactcagatt tcagcttttc agataaatcc tgctcatcca aataatgact tgaaattagt
    19621 gctgaactcg tttctgtatc ctgtacaggc tgagcaccca taccagcaaa aaataaactc
    19681 gttcctagca agaccgaaca agctcctatt gcatatggcc tcaaagaaaa acgctgcttt
    19741 ctctcaaatt gaaattcttt catcccatct cccatcattc attattactg tatattttgt
    19801 atatcagaaa tagtttgtat tcacaaatct ttctagttat tcccttatca ttcctaatta
    19861 agggagataa catacaataa tttttagtta aatgtatatc gatgtttttt gtttttctta
    19921 ataaacgcaa tacaaaaaga gcctgttacc aagctctttg tactcaatga aaatcaaaga
    19981 gcaaattagg aaactagcca caggttgctc aaaacaccgt tttgaggttg cagatagaac
    20041 tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa
    20101 catctatacg gcaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtattagtct
    20161 attatttctt ctcagcgcga agggctgaca agatttgtgt tcggatatca tccacaccat
    20221 ttggagtatt tggtaaaaag atagtttgat ttcctttaga ggcaaaggta ttcaaggtat
    20281 ccaaatactg gttggtcaag aggatagaca tgatttgttc ttctgtcatg ccaacattgg
    20341 cttccttgag ttcggtgata gactctgcca atccatccac aatcgcctta cgttgttggg
    20401 caatccccac accatgaagg cggtcttttt ctgcttctgc ttcagctgca gtgacaattt
    20461 taatcttgtc agcttccgcc aattcttgtg ctgcgacccg cttacgttgc gccgcattga
    20521 tttcattcat ggattgctta acttctgcat ctggttcgac cttggtaatc aaggttttca
    20581 cgataatgta gccgtaagtg gtcatttctt ctgctacttg gtgttgaact tcaagggcaa
    20641 tctcatcttt tttctcaaac aattcatcca aggttaattt tggaacagaa gagcgaagag
    20701 catcttcgat ataagattta atctgagatt ctggacgtat gagtttatag taagcatctg
    20761 tcacgctctg ctcgttgaca cggtactgag tcgctacatt catcataacg aacacattgt
    20821 ccttggtctt agtctcaacc acaatatcac tttgcaacaa gcgcaactga atccgtgctg
    20881 caatcgagtc aatcccaaaa ggcaagcgaa tatgaatacc gctattagca accttttggt
    20941 atttcccaaa gcgttcaata atcgccaccg actgctgacg aaccacataa actgtactca
    21001 gtgtgactat caccaatagg agcacacaaa caatcagaaa aatcatgaaa aatattgcca
    21061 taatggaacc tccacaagta tttttctagt attatagcac atttaaagaa ggctgtgccg
    21121 tttttactgc gatttttcct gaaatgtcaa taattagagg tgaattgtcc tattgtcgtc
    21181 caatctcttg ctaaaataac tctttataaa aggcaatcgt ttcttctaag gttggcataa
    21241 atggatttcc tggtgcgcag gcatcaatca aggcattctt agaaaggtat tcaaagtcga
    21301 aatctttttc ttcaatacca agttcagtca gtttcttagg aatacctact gtctcagaaa
    21361 gcttctcaat ctcagcaatc gcataatcgg cacattcttg atctgattta ccttctacat
    21421 gaagtcccaa ggctttggca acattgcgga aagcttctgg tacacgttta gcattttcac
    21481 gttctataac tggtagcaac atggcacagc acacgccatg tggcaaatta tatactgcac
    21541 cgagttggtg agccattgag tgaacatagc caagaccagc attattaaag ctcataccac
    21601 caaggaagat ggcattgacc ataccttcac gcgcttcaat atcatgtcca ttttctacag
    21661 cacgaggaag atattccttg ataagctcaa tagcaccaat agacagtttc ttggttacat
    21721 cataagcacc aggtgttacc aaagcttcaa cagcatgagt cagagcatcc ataccagtag
    21781 cagcagtcag gcctttaggt ttggaaagca tgagttcagg gtcattaaca gagataagag
    21841 caaggctatt cttgtcaacc attaccatct taaccttgcg ttcttcatca gtaatcacat
    21901 agttaatggt aatttctgct gatgtacctg ccgttgtatt gatagccaca actggcaagc
    21961 cttttttagc agacttatga agcccttcat aatcctgtgg ttttccacca tttgtagcca
    22021 tgatagaaat acaactagct gcatcctgtg gagaaccccc gccaagactg atgataaagt
    22081 cacagccatg ttcttgcaaa gcagctaccc catctgtgac atttttacaa gtagggtttg
    22141 gctctacatc gctaaagatg acatattcga ttccttctgt atccagtggt tttaaagtct
    22201 taggtaaaat atcactgcct tcgatgtact tatctgtcac caaaagtgcc tttttatagc
    22261 caagttcctt gatataagga cccacttcat ttacaacacc ttttccaata aggttgactg
    22321 ccggaacata aaatatagcc atataatttt cctcctgcgc ctccgcgcta tctatttaac
    22381 atcattatat tactttttgt attcgttttc aattatttgt gaattgttta acaaatcttt
    22441 ttaaaaggat aggagggacg taagttcacc tcctatataa acaagtttta tttatgtagt
    22501 ggccccaaca actgacatgc acgatagtct gctgattcta gatcttctgt tccaaataag
    22561 gaccaatttt taggtctaaa gatatcttcc tcaggtacat tatgcatatt gacaggaatt
    22621 ctcaacatag aagccaaggt aatcaagtct gctccaatgt gtccatatgt tatggctccg
    22681 tgattagctc cccaattatt catgacgtca tagacagact tgaaagcacc ttttcctgtc
    22741 aaacgtggag caaaccaagt agttggccat cctggatctg tacgattatc taaagtatgg
    22801 tgaacatctt caggaagttc aagtgtgtaa ccttctgcaa tttgtagcac tggaccaacc
    22861 ccttttagaa gattgagacg taccattgtt actggcatat cccccttcgt caagaaacga
    22921 gttgagaatc ctcctccacg gaagtattcg cggtttgctg gtgggaagtc tgtattttca
    22981 agcatagcct gcacttcact ttcttccaac tcccagaatg gtttcataat aggtttgcca
    23041 tctcgagtag cttgacctgt accatccaat gtacaagaac cagagttgat tagatgtaag
    23101 aagccagctg cagcacgacc ctctaaagta tgtcccgtta cacgtttaac agcctctggg
    23161 ctccaataag tacgcacatc agcaaagatt tgtggagtat ttgttaatag ataattaaag
    23221 agcatagaca caccatttag tgaatcattc tctgtcgcaa atacaaatgg ttttcgaata
    23281 ccattccagt caaactgagt attgaggaaa gtttccataa agtccccatt tggaaaatgg
    23341 tctgtccact gacgttgacc ttggaaacca gctactaaag catggtgacc aaccgcttct
    23401 tcctcaaaac caagttcagc aagtcttggg ttaccaacca ttaagtcacg tccaatcatg
    23461 aacatcttaa taacaaattc ccattgtcta tctttttctt cacggcttaa aacaaggtct
    23521 tcacggttat ggtcgaatcc ttcttttacg ttttctttca cccatttgag cgcacgttcg
    23581 aactcttcag ggtcgtaaat accacggtcc atacggcgcg tgaactccgt catatctacc
    23641 gattcatttc gcattcctaa gtattcttgg aagaaatccg gatttacaat agaaccacca
    23701 atccccatcg aaacactacc cattgatagg taagcagtgt ctctcatcaa gccagttgca
    23761 agagctgcac gcgcatagcg taaaagtttt tctttgacat cttctggaat atctgtgtca
    23821 ctagcttcct gaacatctct tccataaatc ccaaaggctg gaatcccttt ttgagcatgt
    23881 gaagctagta cagctgcaag atagacagct cctgggcgtt ctgtcccatt aaatccccaa
    23941 atagcatgag gaatatctgg agacatatcc atagtttcac taccataaca ccagcatggt
    24001 gtaactgtaa ttgttgcgca aacatttgat tttttaaaca actcatggga agctgcagcc
    24061 tctggtacac ggccaatagt agatggagaa atcacgcatt ccacaggttc cccatctgga
    24121 tatttcaatg tgcttgaaat caaatctgcc acacttttag ccatgttcat tgtttgcact
    24181 tcaagtgatt cgcgtacacc ttgacgacga ccatcaatag tcggacgaat cccaatacgt
    24241 ggatgttgaa tcatactatt ttcctcttat ttttctgttt ctttcatact accatctcca
    24301 ttatatttac ggtagcctgg gtgataattt ttaaaattac ttataagtat aaacctctac
    24361 ctctgctaaa cttaaaggaa ttcctgcgct tctcaaagaa attctaacct tttttccctt
    24421 cagtcctttt aagtctatgg atagattatt gctttctaaa ctgtcaatat gttgtgtaaa
    24481 aacttcataa tcatttgaat catatataat aatatcaaaa tttgataatc tctgaatttc
    24541 agcatctgtt ctattataaa taattaactt ttctaattct tcggtttgtg ctaaatctat
    24601 ctcccaccat gaaggagaat cttctttggt atgagtcact gaatgatgac cataatctcc
    24661 gtttttattt ccatcaactg ctagacgaga aaaaccattg taatccgttg aactctgtcg
    24721 agtttctttt gttaatgcaa tattagaaag tttcttcgga gttctctttg aaccatagac
    24781 ttcaacttcc gctaaactta acggaatagc tgctgatttt aattctattt tgactagctt
    24841 agcccctaca gaaggtactt ttaaagataa caaaccatca actactttat caaaatgtct
    24901 tctaaaaact tcttctcctg atgaagatag aaaaataaca tcaaaattag ataaacgctg
    24961 aggttcggca tctgttctat tataaatatc aactttagaa accgtaaact cttctcccag
    25021 atcgacctgc caccaagcgt tagaatctgc cttagtatga gtcaccgaat gatgtccgta
    25081 atctccatct ttattaccat caactgctaa agcagctaca ccctcataat tggttgaact
    25141 ttgagtagct accttatttg tagagactac tttatcttct gttattttat ctatatcctc
    25201 ttcagattgc ttaccatctg attctctaaa aacctctact tctgctaaac tcaaaggcac
    25261 tccactcgtt agtaatttaa ctttaatata ccttgctcct ttttctttga aatctagact
    25321 aacagattca cccgacaaat tattaacatg tttcttagca acttcgtttc tattattgtc
    25381 atatagaatc acatcaaaat tagatagacg ttgagtctca gcatctgtgc gattataaat
    25441 tttaacaagc ccaactttat ccattttttt caaatcgact tcccaccaag agggattatc
    25501 tgccctagtg tgtgttaccg aaccagagtt aaaattacca tttctgttac catccacagc
    25561 tctattaggg tctccattgt aatctacaga actttgtgtt gttggttttg catatgctat
    25621 attcaaatta ccatcattaa atttatctgg tgttgggaaa gagactggac ctgtcccctc
    25681 agtatttata gacatctcta ccattccatt atgattaacc gtaatggtat aaacatgggt
    25741 attctcatcc caattttctg tataagtata atgattttta tcgccactta tatttatctg
    25801 aggtttatga ccagtatgcc cttttaatgt aagtgttgtt gtcctaaagt cattatctac
    25861 aggattgatg gaataatttt tgcttatcca gttcgctaac tctaattctt ctttcttcca
    25921 actttttgat gcatcaaaat ttcctgataa tgcccacata gctgtcttat ctgtcctgta
    25981 attattcaat aaaatatgta aattatttgg attttcttta acaacagcgt aagtatgtgg
    26041 cgtcaaatct aacgataacg actttgtatt attagtatac ataggcaaca ttacttgctg
    26101 atttttattg atattagcat tactattata aatataccag gaattaccta cacgctgagc
    26161 atacccatct ccttcataaa gttttggata aagcgagttt aaatagttga ctttactaga
    26221 caattcctca ctatttttag tcaaaatttt tgcattaggg aatatagatg aaatcttttc
    26281 cttatcaatt ttctcatgta ttacaggaag aatatgatat ctcccattat tatataaagg
    26341 cattgtttca tcattcgaat aaagtccttg ataaaatccg tttaatgaac taatcctacc
    26401 ttctccattc caaaatacag cttttgttct atttacaact tcttccttac ttggagctgg
    26461 attttgtata gcatgtctaa agaaaggaat aataccttta gtaaatgctg gagttggtac
    26521 atcatttgtc ataaatgtat acgcggcaca ttcgaaatta taaactgtgc ctcccccagt
    26581 atatacattc atcatttcca tagcaatcat tgattctggt tccgatgcat aggatctcat
    26641 atccctagct cttccagttt caaatgtgtt tgtataatgt ttttcccacc atttccatgt
    26701 atctgttgat gagccccagt tatcacataa gccactcaac caaaatccac taacgataga
    26761 atctgtaccc gcatcatctc ttattggcgt atttttagtt gccaacacca aatttttatg
    26821 atatttttga ctcgcttcaa agaatgtcgg atcattcata atagtttccc agaaccattt
    26881 ttcatgatca tgccagataa aatgcgctcc atatttggca caaacttcca aatatttagc
    26941 actatgcgga gctaactggt tattgtaaat ccaataattc tcaatattta aaacaccttt
    27001 taacacacta tacttttgga attgttcatc taaccactct ggaggaactg tattacgctc
    27061 tccagccgac ataataacca agaaaactgg aatgtttaga ctttgagctt cctccaacat
    27121 cttgacatac cattctctca attctttcgt atctcgtgga atacaacttg ttggtttaca
    27181 gacttttgca ggatgaagtt caatcgctgc atatggcttt acatcttcag gaatagcttc
    27241 ccatgctccc ttcaatgtgt tcccccacca taatccgtta ccattatcat tgccatacaa
    27301 cggagaaagc aacaatggcg attcattatt aatcgtcgtt ctcatttgaa cacgattatc
    27361 agccttaact cctgctaaca atccaaaaca taacatccca atacatacaa acaatattgt
    27421 gattaatttt ctttttattt tttctttatt catgacttat acctaaaaat ttaactcaat
    27481 aattttttct gaattaaata ccaattcaat attttgtaca tcagtatttt tgccatatat
    27541 tcttactctt actttttgat ctttgtttcc tccttccaat tttaggaatg ttatatcccc
    27601 atttttccaa gcaaacgata ccttatatcc tcctcttact ctgaaacctt tcacttctcc
    27661 ttctgaccaa gcagaaggta aagctggaat tagtgataac caattatgat ggctctgtac
    27721 taataattca caaattccac tcaccaaacc taaattacca tcaatttgaa atggtggatg
    27781 gtcaagaaat aaattgccaa gagtcgcatt atttaacaaa ccattaatct ggttataagc
    27841 aggttcacct tgatatagtc tcgcaaaaaa atgaatcagc catgcagcac tccaacctgt
    27901 ttgtgtacta gcatgcaaac cacttactaa ccaattatta atcgcttgct ccctctcctg
    27961 tgaagataaa aaattagcgt ttgataatct cctattgata gtgattttag ctgcttctgc
    28021 taattccgga gttttatgaa tatcaatctc attataagga taaagcccaa atagaggtga
    28081 aatgtgtcta tgcccaggct ctacttcttc ataatcttct aaccattctt ggatttgccc
    28141 attactacct atttttgttt taggtagttt cttttttaac tccttcacac gactaataaa
    28201 atccgaattg tctcctaatt gttttgcaat gccaatgcat gaatcacaaa aatatcttag
    28261 aatttgatta tcaattgtag atgatagaca agcatttcct tcaataccat tttttaagcg
    28321 atatttattt tccggtgaga cacttggacc tgtcatcaag tagccatcca cctcaaataa
    28381 ataatcttca aagaaaagaa atgcttcttt tatcatttca aaatgttccg taagaatacg
    28441 ctcatcttgg aaatataaat agtgttccca aatatgagta cataaccatg gaatagttaa
    28501 tacccaaatt gcagccccca tggcatgaga ttggggagcc gtatcgccaa aaccatccgt
    28561 attatgatgt gctgtaaaac ctctagctcc atacattttc ttagcggtta gtcttcccgg
    28621 ttctctcatt ctttcgagca tatcaaataa tggatattct acttctggta aatcacatgg
    28681 acctaccatc caataattca tttgagtatt aatattaatc gtatatttag aaccccaaat
    28741 tggatttaat tcatcacacc atattccttg aagattggca ggtaaaccat tcggttgact
    28801 agacgatatt aacagatatc ttccataatg aaataacaag ttagtcaagt agttactata
    28861 ctttttagtg ttttcaagaa gtagattcgt tggaatgcta agacaacctt tactatagtc
    28921 tagtttaaaa tcaactctat taaattgctc ctgatatttt tttacatgtt catctttttc
    28981 tgtaaagtaa tcaatactac taaattctcc ctgaagagaa gaaatatcta tatttcccca
    29041 ataatccgtc attgatttga gataaagaaa tacctctgta gcattccgaa taactattgt
    29101 ctctcccaat acacttactt caccatccgt aaccttagaa tgacatacta ctttaaactg
    29161 aacacctttt ctacctccag cagaggccga cattaaaatt gtacttgaat ccagtttaga
    29221 tacttcgtca ttaaaccgtt tatttctacc caaattaatg tttaaattta atgtgttttg
    29281 aactgatgac actatacggc aacataaaat attcttatta aaactcgtaa aatattctct
    29341 ttttatttgt aaattacaac tattaggctc aaacacaaca ttagaaatag ctgtatctaa
    29401 atctagctct ctttcataca atgaaagagc acaagactga atatctatat gctcaatgta
    29461 aagttcccca agtaattcat agtggctttg atctcttggg gtagcaaaca ctgttaactt
    29521 tatcaattct tcggctttct gaatttctcc atctaaaaga tattcccgaa tttttttaag
    29581 atgcaatagt gagtctggat tatttctatc tgactttcct ctataccaaa tagtctcatc
    29641 gtttagttga atacattctt ttgtagctga accataaatc ataccaccta aatgaccgtt
    29701 cccaatcggc aaggcttcat tccaatttgc ggcagctttc ttataccata atttcactct
    29761 ttacctctct tccaaaatgg aggtgatatc ccaaatacca cctccacact ttctactact
    29821 tcaaaattcc taaaacaact agaataactc caatagccat aacactaaag attagtacaa
    29881 ccggattctt tccttttcta attaatttac acattaataa agtcaaagcc atagggataa
    29941 atcctggaat taattttgta atcatttctt gaacagaaat aacaagttcc ccctgcttaa
    30001 attctaatcc aaaattaata ccaaccattg atggaatcaa accacccact agtactagcc
    30061 ctaatgctgt cgccatactc gtaacgcgat tcaatgttcc ttttgtatta ttttcttgga
    30121 taagactaga acccttagta tacccatatt tcaaaccgaa atatttaaca ggaatattaa
    30181 taatattaaa caatattaag gcgataaaga tacctaaagc accgccgtct ttagaataag
    30241 acgcaccaat actaaaacag ataggaacta gtgtcagcca gaacaaacta tctcctagac
    30301 cagccagtgg ccccatcaag ccaactttaa taccagtaat tgaagctgca ccttcatttc
    30361 cttcttgttc ttccatagcg gaagtaactc caagaataaa tggtgccgct gtttgatgag
    30421 tattgaaaaa ctcaaggtga cgtttcattg caggagaagc tgaatctttg tctgggtata
    30481 gttttttcaa tactggaaga attgtatata gaaaacctag gttctgcatt ctctcatagt
    30541 tccatgaaga accatacata aaacttcgtt tgaaaacttt tttaagttca actttagtta
    30601 ctttattatt catcatagat caagatcctc ctcaacatca ctacttgaag ttgtttcttg
    30661 tggtttgctt ccaatcatat cgtagaagta tgctaccgca ataccaacta gtgagatccc
    30721 aatggttgac attcctccat acacagaaca aatgaatcca atcaacaaga atacccacat
    30781 atttttcttg agcatcatat ttaaaagcat accaaaacca attgaaggta gtaaagcacc
    30841 tgcagagtta agtccctgca taacgattgg tggaacctta gcaaacaagt ctgccacata
    30901 ttcccctcca agcataatag ctaggaaaat tggtacaatt ttaaataacg tgattaaaac
    30961 caaacttgaa taatgataaa aaccgatctt tgaataatct ccgtttttag catcattatc
    31021 agcttttttc acaaaataaa catctaaagt ttttgcaaga acatccaact gttgggtagc
    31081 aactgcaatt ggaactgcta tagcgatacc agcagtttct ccttgaccag ataaaatacc
    31141 aaatgcagta ccaataatcg cacctgaaat agtatctggt ggagtataac ctccaatacc
    31201 tgttacaccg agccaagtta attcaagagc tgaaccaata agaacacctt gggtgaaatc
    31261 tcctaatact aagccaacca ttgcacttgt aactaacgga cggtgaatca taaataacgg
    31321 tccattatag tcaatagtgg caataatccc aactaatgtc accagtaatg cttgtgtgaa
    31381 tagcatatac tgtacctcct taaattattt ttataaaaat ttttccaaca ttgtaacttt
    31441 atcattagga accatttgaa tttcaactct cgttccctta tcaactattt tcttaaaata
    31501 atatttgtct tcttcaccta gagctacacc tcccgttacc ttttcatttt cctccgtttt
    31561 actcatctgt cctacattga ggtactccaa ttttaaattt ccttcaatag aatcatacac
    31621 atcttttgga tttgtatata tcagcattgt tctctttttt attggcacag agtttaaaac
    31681 ttccacaaac cgttttacac taaagaaaac aatttttaaa cctaccggtg cagaaatctt
    31741 taaaatagat tgtcgtgttt tatcttctga gatgcgatca ttaacaatga taacttgctc
    31801 aatatcatac ttttttagcc acgtagtgac aacttgacca tgtaccagac ggtcatcaat
    31861 acgaacaaac tctatagaca taaatcttct tcctcctcta cgttaagttg ttgtttaaca
    31921 ttaaacaaac tattttgagc attgtgaaca atttcttcta aattgatttt tgaatcataa
    31981 cttgatatta attccactag gagagggata ttaaaccctg ttacaatatc aactgaatcc
    32041 aaatttaaaa accgtgacaa agccacatta ttaggacttc ctccaatcaa gtcagtcaaa
    32101 acgataacct cttgatttga gtctaacagt tcatccactt gatttttaaa ataatgctca
    32161 aactctacaa tattctctcc aggcatcaaa cctaatgtcc taactctatc atttgttgtc
    32221 tcaccaacaa acatttctgc ggtcatgaga gctccgctag caaaattacc atgactggca
    32281 actaaaattc ctctattgaa cattttctct ttcaacaact actcctttct taaggataat
    32341 attagcataa agtgaagttt ctcctgtagc aacaatagca taagctttct tactacgttc
    32401 ataaaagtct tctcttctaa gataagtaat cgttttaaga tctgtaccat gaccttcaat
    32461 catctgtcta taggtacccc atatcttagg aatatcatca cccgaaacaa cgttcataaa
    32521 ctgaattgaa ctatcgacgt aactatctaa tggcattaaa tacagaatgg aatctaataa
    32581 ttctggaata tttacaccat cacaacgaat tagcttattt gcacatgagg cagaaggata
    32641 attcgcgtca gctaatacta tttcatctcc atgtcccatt tccattaaag tcttcaataa
    32701 atctggagaa atatttttcg gtatatgttt taacattaat atcctcccta ttttctctga
    32761 ccgtatgttt taaatttttc tgccatcaat tccatctcct catcaggaag aactactggc
    32821 tctccaaaat tcttagctaa acaataaatt tttgcacaat attcaacttc ttcaacaata
    32881 ttaaatgcat tcaataaatt ttgtgcacct gctaaaattc catgattcgc tagtaaaact
    32941 gctctacgac cttccattgc tttagctgca ttcacagcca attctttcgt gccatatgtt
    33001 gcatactcag ctacccgaac atctttccct gccactgcaa tcatataatg actcgctgga
    33061 agtggttctc tgagacaagc taatactgtt gcataagttg tatgagcatg gataattgca
    33121 tcgatatcat cacgagtttg atattgaatc aaatgcatat accattcgct agatggcaag
    33181 cgttctccct ctacaacatt tccattaata tccatcacta caatatcgga ttctttgatt
    33241 tcaaagaaat caatacccga cggggtaatt gccatcaatt gtttttcacg atcgaaaacg
    33301 ctgagattcc cacctgttcc tttcgtcaaa tctgtttcta ctagcttctt accatattta
    33361 attaattctt gttttacatc tgacatcctt gtcctcctca tatcaaaaca ctaaatattt
    33421 ttcaattcac caattatccc cccttcctgc acaaaagcta agaaggggga gggaggcaaa
    33481 tttacaaact ctcaatcttt ttaattcttt tgggagtaat aactctctaa ttgtagaaac
    33541 tcctcaataa catggtgagc ctcttccatc ccttttaatt gtcccatagc tatgagctgc
    33601 acaacaatat tccccacagc tgtagcctca gtcaaacctg taagaacctc tttaccagtt
    33661 ctatcagcaa tcatttggtt aaagtaactg gctctagcac ctcctccaat cacatatatc
    33721 ctcttataaa ccttatgagt tagttcttct agtaactcta tcgctttcct atacgtttca
    33781 gctaggcttt cataaacaat cttaaatagt tgtccatctg tccactctct agtttcatga
    33841 tgataagcta gatattctgt caaagtcttg tgcatatcag attctgttgc aaattcagtt
    33901 gattcagtat caatcagagg aagattttct ttttctttct ccaccattgt cctaatatca
    33961 tcaaaagaat aggctttccc tcttcgttca aatgaacgtc ttagttcctc tatgatccac
    34021 aaccctgtac aattcttcag aaatgtaatc actccatctt taccgacttc atttgtaaat
    34081 ccataactga aggattcggt agtaagaatc ggtgaagtaa gttccactcc aaccaaagac
    34141 caagtacctg atgaaataaa taaactacct tctgtcttag gtactgagac aatcgcgctt
    34201 gctgtatcat gactacaaac attcacaaca ggaatatcgc ctaaaccata ctcctctttt
    34261 atccttccaa gaacatttcc ctctgaaaca atttcaggaa gtaaagatga atccaattca
    34321 aatagtttta agatattctg attccaattt tgactcctag gatcaaataa ttgagttgtt
    34381 gaagcaatgc ttttttctgt agcaaactta cctgtcaaga gataattaaa caaatctggc
    34441 attaaaagaa tcttattggt cttatagaaa gagtcaggag attcttgacg tgccttaaag
    34501 agttgaaaca aggtatttat ctccataatc tgatttcctg tctctgaata cagtttttct
    34561 aattcagtca tttcagatat ttcctttaac actccctttg ttctttcatc acgataatga
    34621 acaggttgta ataacagctt accttcatta tcaatcagtc caaaatcaac tccccatgtg
    34681 tcaataccga tagataaaat cttgtaacta gtattagcca agcggatact ttcaagaatt
    34741 ttagctagta gaaagtcaat atcccaagat aaatgccctt ttactctaat aggtagatta
    34801 gaaaagcgat ttatttcttc cattactagt ttattttcag aaaggtaacc aacgattgct
    34861 cttccagaag tcgcacctaa atctattgct aaaaccgctt ccatttttct tctctcttcg
    34921 tccttgatta actttattat aatccccacg ttttattttt tcaatatgtg aacgcttact
    34981 tgaataatgt tcaaatgtta acccaatatt cataaaaata attcaaattc ctccaatttg
    35041 tgctataata tagttgaaat gataaattag gaggagctta tgataaaaga tgaacgtgta
    35101 cttgaattga ttgaaattat caaaaagaaa aaaagaattg ccgtaaaaga gctggcagaa
    35161 atcactttct ccagcacaag taccttacgt cgtgatttaa ttttcttaga aaatcaaggt
    35221 cttatcaaaa gaaagcacgg atacgtgacc ctgtcctcta tgaacacaat tgaactttct
    35281 catcaaatac gtgaaggaga aagtactagg caaaaaagac taatcgctag tctcgctaaa
    35341 gactttattc ggtctggtat gtgtatctat ctagattcta gtactactgt ctacgaactc
    35401 tgtccctatc tttctgaact tgataatttg attattttta caaatggttt acatactgca
    35461 caaaccctat ctgaaactgt taaagatagc tccaaaatct ttatcacatc tggcgaggtc
    35521 aaacatcaat cctgttccgt ggtcaactat gataaggaaa attctttatt agatcatttt
    35581 aatatcgatt tagcattttg ttcagcaaga ggtattgatg accaatatgt ttatgaagct
    35641 tctctcagcc aagctatttc aaaaaagaat attattgaca aagcccatga aaccatctta
    35701 ctgattgata gttctaaatt ttacaagact ggatttttta aaattaatcc cctctccaaa
    35761 tacacaacct ttatttcaga caccgtgcca gatcaaaaat tattagacgc agtcgaatta
    35821 tttgatggag aatgggtttc tgatattcaa tgaaaatcaa ataggaagct agtctcagct
    35881 tgctcaaagt acagatttga aaattgatca agtcagctcg aaacactgtt ttgaggttgt
    35941 agatagaact gatgaagtca gtcacatata tacggaaaga cgaagctgac atgatgtgaa
    36001 gagattttcg aagagtatcg ttctgtctgc tgactgattt acaaatattc accacatgac
    36061 attattttct tagcaaccct aaaaacagag gctggacaaa aactcaattt caggagaaaa
    36121 tggagtaaac cttcccacaa taaaacgcat agtatcaagt ttttgcacac ctgatattat
    36181 gcgtttttct gattttaaag atttttctct tagatctgat atttcaagaa ttaagtgacc
    36241 ttgatgatct aaatgtattg tggtatacta tttttgtcat cggttaccga ttacgttcct
    36301 atgggttcgt gagaggaggt gaaactaatg agccttatca tagagctcgc tcttactatt
    36361 atagcggatg ttatagctgg aattatcttg tatttcgtct gcagatggct agatagtaag
    36421 gagtagaccg actgactagc ctataaacac ccgttaaatc gctaagatac gtcaaaaaag
    36481 cccttaacta cggccatagt taggggcttt ggtgttctaa tgaaccttat caattaacta
    36541 cattctagca tataatctac aatagttcaa gcattaagta aggttgatga cctgaatgta
    36601 gtgtgatata ctatttttgt catcggttac cgattacgtt ccttacggtt cgtgagagga
    36661 ggtgaaacta atgagcctta taccagagct tgctcttact attatagcag atgttatagc
    36721 tggaattatc ttgtatttcg tctgcaaatg gctagatggt aagaagtaga ccgaatgact
    36781 agcctataaa cacccgttaa atcgctaaga tacgtcaaaa aagcccttaa ctatggcact
    36841 agttaggggc tttggtgttc taatgaacct tatacactaa ctacattcta gcatataagc
    36901 ccagatattt caagagtttt atttattgtt taaagttctg aaaggtctat aatgaagtta
    36961 gccatctagt atcaaaaaac cgactagctc ttatgaacta gtcgatttct catcaatgcg
    37021 ccaacatttc ttgggcgatt tcttggccag ataggttatc tgggtagtag gttggccagt
    37081 tgtccatttc ttcaaagagg gcttcttggc ttgtgcctcc aaagaagata tggaaatgtt
    37141 ctgccttaac tggggcaaca ttgtggtcac taaactgaac atacttgaat tgtccagcgt
    37201 cagcatctgt ggcttcaaag aggaaacgca cgccacgatt gcctttcttg taagtcaaaa
    37261 ttttcttacc gacatacttg taagtgtatt tcttgctttg tccaccttga acaaattcca
    37321 tagtattatc agtaatgtta atcttagtca catctgtatg atagcctttt gtatagtaag
    37381 ccttgtactc agcctgggtc atcttaccag tcaacttagc cttgtagtca aagacttggt
    37441 caaacgtgcc gtcttcaagg aaaggataaa ctgattgcca gttacctgca tagtcactca
    37501 aggtgcggtc cttgacagct gcatcctcga agtaaccatt ttggactgtc ttggtatcct
    37561 ctgccttttc aggttcaatt gctgggcctt cttggtctgt tgtttgtttc aaagccttga
    37621 ggtttttctc catcacggaa atgtagtttt ctccagcctt ggtgtcctct tctgtcagac
    37681 tttctaaagg attgaggaca tcagttttga cacctgcttc ttttgaaagt gtgttagcaa
    37741 gggcttgtga ggcattttct tcaaaataga tataggcgat tttatttttc ttgacatact
    37801 ctgtcaattc tgccaagcga gcagctgatg gctctgcatc tggagaaagt cctgagattg
    37861 cgacttgttt gagtccatag tccaaggcaa gatagttaaa ggctgcgtgt tgagtcacaa
    37921 agctcttttg ttttgcttga gacaaacctt ctgcgtaagc cttatccaag gcttgcaatt
    37981 tttcgatata ggcagctgca ttcttctcaa aggtctcttt tttatcagga taatctgctg
    38041 acaagctgtc gcggatgtgc tctactagtt taatggcacg aactggtgat aaccaaacat
    38101 gggggtcaaa ctcatggtga tgaccttctt ctccatggtc atggtctccc tcttcttcct
    38161 cgccacctgg caagagcaac atatcgcctg tcgccttgat ggttttcact tttttcttat
    38221 ccaaggtatc tagcaattta ggtacccatg tttccatgtt ttcattttca taaacgaagg
    38281 tatctgcatc ttggattttg gcaactgcct tggcagatgg ttcgtattca tgaggttctg
    38341 tcccagcacc gattaggagt tctacattag ccgtatctcc tgcgacttgc ttggtaaatt
    38401 catagacagg gtaaaaggtt gtcacgatat tgagtttacc atctgcctgt ttttgattgg
    38461 aacaagccac taaaaacaag gcacatagac tggctagtaa taagctaatt tttttcacgt
    38521 tcgtctccta tttgataaaa cgtcttacta aactgattag tataaagaca gttacaaaaa
    38581 taatggtaat acttgcactt gcaggtgttt ctgcatagta ggaaatgtaa agtcctgcta
    38641 ccattcccaa aaagccaatc gcactggcaa gcagcataac cgatttaaag tttttcccca
    38701 gacgcagggc aatactagct ggcaagacca taatggtcga taccagaaga gctcctgctg
    38761 caggaatcat aagggcaata gccacccctg tcaccatgtt aaaaagaatg gacatggtac
    38821 gaactggcaa gccatccaca aaggccgtat cttcgtcaaa agttaagata tacataggac
    38881 gaagaaagag aaaggtcaaa atcaaaacaa ccgccgcaat gacaaagagg gaaatgacct
    38941 gttcttcact gatagtcacg atcgaaccaa agagatattg gtccaaactc attgaactcg
    39001 agcttttacc cttgctcatg acaatcagag aaacagccag acctgttgac atgaggatag
    39061 ctgtcccgat ttccataaag ctcttgtaaa ccgtacggag atactccaga aagaccgccg
    39121 caatcaagac aatggcaata gtagaaacag ttggagaaat ccccaaaacc agaccaaagg
    39181 ctacacctga aagtgagacg tggctaaggg tatcactcat caaactctga cgacgcaaga
    39241 tgaggaaggt tcccaatacc ggtgagaaaa gactcatagc aataaccgcc aaaaaggcgc
    39301 gttgtataaa gtcgtaagat aataaactaa gcatggccca cctcctggcc attctcatga
    39361 acattgaaac aacgccatgg cgagtcttgg ttacggacta gatgaatatt gcgatccgca
    39421 taatccttaa cttcttcagg gtcatgggta atcatcaaaa cagccttgcc atgatgatgg
    39481 gcgctgtggt gcatgagttc gtaaaattca tttttacttc ctgcatccat ccccgttgtc
    39541 ggctcgtcta ggataaacac atcagggtca gaagcaaaca tacgcgcaat taccgctcgc
    39601 tgcttttgtc ccccagatag agaccccaag cgtttgtctc gatgttccca catgccaact
    39661 gagtccagac tagccttgat atgctcctca tcatgagcat tcaaacgacg gaaccagcct
    39721 tttctcggat agcgacccga cttgacaaat tcatagaccg tacttggaaa accagcatta
    39781 aaactggcaa tttgttgagg aagataggct attctcaatt tcttaccttg cgtatttgtc
    39841 tttgaaatag ccacctttcc aatgcgtggt tgcagaattc caagactagc cttgatgagc
    39901 gtcgtcttag ccgctccatt ttccccagtc aaggtaacaa attccccact atcaacacaa
    39961 taattgatat gttcaagaac aggctcctta tcataataga aggacaaatc ctctaccgta
    40021 atatatctca ttatttgatt tctcctacta aagcagtcaa aaaccgctga atcacttttt
    40081 gttcatttgg agtaaactga gtcgccactt gttcataggt taaaagtgta tgctcatggt
    40141 gatggtggtg ctcctcagcg attggacgag ccaagtcagt caactgataa aaaatcacac
    40201 gcgcatcttt agaatcttta gatgtttcca acatcccttc cttgaccaaa gacttaatgg
    40261 ccttggtaac tgccgcctga ctgacattga gacgacgggc caattctgaa tttgttaaag
    40321 attcctctga caagagcata aggatatgct cctgagtatt ggtcagggcc acctcgctag
    40381 tgcaatgacc tattaggatt tcatgctgat tttccgcctg caaaatcacc tcattcaaaa
    40441 aagcattgat atcctttgct agctgtctca tatctgactc ctttcctttt agacttctct
    40501 tttttaagag aaaaatacta ttctttgaca ttttgtttac cagttaatta tatcacaagc
    40561 aaaaaaagag tcaagaaaaa acgtgaaaac tagtttcatt cttgaactct tctatattat
    40621 attatctatt gaaattcttt gacatctcca tcataagtcg cccaatcttt gctgaaaaag
    40681 cgctcattca gatggtaagt cggagctggt gtgggattgg ataggaaagg atcaactgcc
    40741 ttgtcaaaag ccaaccaacc caaccaacca aggtgaatgg tgtccttcat aaagaaaggc
    40801 tccccgccgt ccttagaaaa atctgctata ttggtaaaac cttgactttc taactggtag
    40861 cgaatcttct gcaccgtttg ttggtacata tcctctcgta gaccagcata gttcatccat
    40921 tttttattaa caggtggaat gataaaaatc gggtttacct tagatttaga aaactgtgtt
    40981 aaaaccaact gcaagtcatt atactctggc gacttgagat aggtaaagct tttctgagaa
    41041 tcctttaatt tcttcaaatc cttcttgatc tgctcattat agaaataatt ttccattccc
    41101 atctcattat tggaagtatt tttttcagca tctgctttga caacatcttc tattgcctga
    41161 taagaaaact ggtctggcaa gatttttaaa tacttagcta catgcttatc gtagttaaca
    41221 tagcctctaa ccgaaaactg accaaaaaag gaagcttggc gttcattaaa acgagccaat
    41281 aattcaatca tttcattgtc tgctgtcgac aattcttctt tacttgccaa cttctgaacc
    41341 aggtccttca tagctacgtt tgggaactgt tgcagtaagc gagtcgctgc atattgacta
    41401 gcctgatccc cagattgatg tttcagaaaa ctagtcaact ggtctccatt aaaatactgc
    41461 tggaaggctg ctggatcata gccattttta ctgaaccact gaggtgagat aacatacaca
    41521 acttgtttat tctccagctg tggtaacatc tgttgcattc caaaatattg gttaagcgat
    41581 gcagctcccc cctgtcctaa aagataagga cggtaggaac gattgtattt ctcagctaat
    41641 accgcaggat gagcaccgtc aaaacgaagc cattcactag agccaaagaa gggaacaaaa
    41701 cgcacatttg gatcagatag tgctctgact ttttgacttc gctccttaaa actatcgata
    41761 gtagtagcca ctgctgaacg cttttcagct cctagattat gatgcatctc agtaggataa
    41821 aagaaaatga gcagaaaaac caacaaacca gcgatcaaga ccggtccgaa gatcatccat
    41881 aagcgtttaa gcattttgta gctccacaat accagctatg attttattag ctgtattcca
    41941 gtcgtcacga ccaaactctg ttacagggac acgaatgtca aaacggttct caatctccac
    42001 aatcaactca accgttccca tactatccaa gacacctgca tcaaaaagat cttcatccat
    42061 catgtcagaa acatcttcca taaacaactc atcaataatt tcaataactt ctgatttgat
    42121 atccatattt tatttccttt tattttttaa accatagatt attcaagaat ccagaaaaga
    42181 ttaagaatga caacatgaca acatggaaag tgacaaccat gccaagcaac tgaatccagc
    42241 gattctcagg tagggcagcc ttccctgctt ttttccgttc cttattgagc gtttttttct
    42301 tgcgaaccca ggcatcattg atgaccaagc ctagtccatg aaagagtcca taggcgatat
    42361 agtaccaggt cacaccatgc caaaatccca taatcagcat atttacaatg taggccatgc
    42421 ttgaggttac attacgattt ttaaagactt tctttctggt taacaccatc accattcgca
    42481 taaagacaaa gtcacggaac cagaaggaca gactcatatg ccagcgattc caaaactcct
    42541 ttaaatccct tgataaaaag ggcttgttaa agttgatagg gctacggatt cccatcaagt
    42601 ttgagatggc caaagcaaac atagaataac ctgcaaagtc aaagaagagt tccagaccaa
    42661 aagtatacat aactgccaag gcatagagat taaagaagcc acctgactgc aaggctaaat
    42721 tcttcagagg aggtagtaag gtctctccta aaacatgagc taggataaac ttatacaaaa
    42781 agccccacat gatatagcgg acagattcat ccagcatatc catcaactca tctcgctcag
    42841 gaatagcctg ataattttca ttaaatcgct taaagcgatc gattggacca ctcgagaaag
    42901 ttggcatgaa gagaaggaaa cggaggaatt cccagagggt aaaatcctta atcactccat
    42961 ctctcagctc gatgacaatt ccaaccgaac gaaaggtcag gtaagaaatt cccaagaacc
    43021 caagcaaaga ctgcgttcca ttgatagctg gttgcacctt gacaaagata atcggaagta
    43081 gggacagaaa actaactaag tagaagaccc acttgccatc cttgcttttt cgataatgct
    43141 tgtagaaaag caggagcaat atttcccagc aaaggtaaat acccaaggca gctagttgat
    43201 tggtctttcc acccaccaac atggtgacaa taaagaagag acttaccaac acttcatacc
    43261 aggcaaagcg tttcttgaaa aagagaccta taaagatggg caaggttgca gcaatcacat
    43321 aaacaaaata ctgaggattg ccatatggct ctaaatgagg aagctgttga aaaaactcca
    43381 tcatctctta ttcacctcgt taatcaatcc tttgatgtca atctttccat ttggagttag
    43441 tggcaaactg tctcggtaaa ggaatttaga tggcatcata taggacatca tgatgtctgt
    43501 caggtcttcc ttgatggcct tggtaatatc gatatctcgc tcaaactgct cacgaacacc
    43561 gtcttttaag atgacataag ccaatagatt ttgtaccttg tggtccttgt tatagcgcgg
    43621 tactgcgaca gcagattcga taaagcgaga cttgttgagg ttttgagaga catcttctaa
    43681 ctcaatgcgg taaccgttaa acttaatctg gaagtccatg cgtccgccgt agagaagcaa
    43741 gccctcatct gtcatggttc ccacatcgcc tgtgtgatag gctggcagat cttcaaactc
    43801 aaagaaggct tctgctgttt tttcaggatt gttcatataa ccttttgaaa cagctggccc
    43861 agaaacaatg atttctccct gctcaccatt tggcagttta tttccttcct cgtcaatgat
    43921 aaaggttgga gaatcagcct tggtatagcc gattggtagg cgtttgagag tcgctaacat
    43981 ctcgtctgtc acggcaactg ctgacagagc tactgtcgct tctgttgggc cgtaagcatt
    44041 gatgatacgg gcatttggga aacgctcgcg cagtttttga gctgttttga ccgtcaattc
    44101 ttcaccatca aagtagaaat gcgtgattcc aggcattttc tcactgttga agtattcaga
    44161 caacatggcc atatctgcaa aggatggtgt tgatgtccag atagcgattg gcaatgaaaa
    44221 gatagccgca aagagttgct taaagtcctg agtgatgact gaaggaagag tgaaaagcgt
    44281 accaccaagt gccaaggtcg gtgcccaata catgacagac aagtcaaaag aataaggtgg
    44341 ctgtgccagc atttgcggac gactcggtgt cgcaaattcc ttatccgtaa tcatccagtt
    44401 tgtaaagctg aggagattat catgtgaaat ctgcactccc ttaggcttac cagtcgtacc
    44461 agaagtaaag ataatgtagt aattatcatc tcccttgact ggatgcgtga tttcatagtt
    44521 attcccttgg gcaaaggctt cttgaacctg agctagattt atcattggtg tagaaacctg
    44581 ctccaaggga aaggctgaaa tggcaataat caagcttggc tctgctactt ctaaaatagc
    44641 tgaaactcgc tccaaggccg aatggctatc aattggaatg taggcatgac ctgacttagt
    44701 cagcgctaca aaggttgcca acatttcata ttcttggcca ccaaaaacaa ccacaggaga
    44761 cttctcaggc aagcctagtt ggtcaatgac tgcagccaaa ctatccgaat cagcctttaa
    44821 atcgccataa gtgtgttcct gccccaaaac attatagaca ggatagctag gctgtgtctg
    44881 agcaaaatgc tcaatggttt caatcatatc tgctattggt ttatttgaca caatagggat
    44941 tctccttcaa gttaaaattc attatagata aagcttcctt gaccctgacc aagatagcta
    45001 aagaagtaaa gcagccctag aaagataaga aaatacaagg ctgtccgacc aagaaagagg
    45061 tacaattctt ttctctgttt catcaagaaa aaccattcat ttctgtaatt tttcgctaaa
    45121 ataagagtga ttcttactag cttatttttc taccattgta ccactttata tagtatcttt
    45181 tcaattgttt accgtatgtt tccaatagat ttcagcttat tttaaggatt atacagtttt
    45241 tctatgtata ttttcaaata gagtgatcct gcttcaaaac tccatttcag gagacaatga
    45301 agtaaatctt cccataataa aacacacaat atcaagtttt ttcaacacct gatactatgc
    45361 gcttttctga tttttaaaga ctttttaacc actctctcat ttaaaataat ctcgtctgat
    45421 ataaattaaa atagcttcta tcatcagaca aatggctgat agccaaaaac tgatgctaat
    45481 accaaaactc tcagtaatat agctcattag caaaacaaat actgaaaatg ctaatgtaga
    45541 aatcacttca agaacggaat agacattaac taaatgattt tcctctactg tttcctgaag
    45601 aaatacactt tcaggaactt cttttagttg cgataacata ccaactaaag ctgaaaataa
    45661 taaaaacatc tgtgcgtttg gaaaatatag aatagtcagt gtcactattt ccatagctac
    45721 aagaggaaaa agaatacttt cccatttagc agcaaggaac ttttcagata gcctaaaagc
    45781 aattaagcca ctaattataa taccaataga gtatgctgta ttagaatatc cccagtaact
    45841 ttccgtttta tttaataact ccgttacaaa aacaagtata atggaagaaa cccaaatcgt
    45901 atttgaaaaa atttccaata aatttgctga tacaaaaagt cttaatctag gatttctagc
    45961 aactaacttc caacctttga gcaaaatttc aagattagtt tctgactcta acacctccac
    46021 ttcagcgtta ggaagaaata acatcagaaa gctagaaatg atatacaaga ctaaattgat
    46081 acacgtggta ggtaacagac caattgttgc aaacaagagt ccacctaatc cccaacctat
    46141 caattgaaca gcttcaccag tcattgataa ggctgaatta gccttaccca aatcggtcgc
    46201 atagcgtggc acaatagcat aggaaacggg tgctgcaaaa ccatctagta tggaaattgc
    46261 aacaacaaat agataggtca ccaaaggcgc tacggattgc attacggtaa acattcctac
    46321 cagtatcgcc aataatatag tctttccaaa ttgagataaa gataaaaccc tatttagcgc
    46381 taacctttta gtaaccaacg gaactaaaag actcgcaaca aaagaggata ttcctattaa
    46441 gataggaact agtgatgtgg caattactga ttttgaaata atgtatatgt tagcaatgat
    46501 tgttactcta aagaaaatat ctgctaaatt tgcaaataat tgagagacta acaacttgac
    46561 aaatgaatta gacatgtagc gctccccctt tagttacaaa ctttggaatt aaatttgatt
    46621 tttaatattt tagcataaaa tagcagaaaa caaaagtgag gttttttaca tgaaaaaaca
    46681 aaaactattg cctttattat tcctaatttt aggaggaatt atgattatcg ttgttggcta
    46741 gtactaagaa gttagttagt tagtagaata attacatctt caatcgaaaa agagactgga
    46801 aaaagtctta aaaatcagaa aaacgctcaa atcctttggc gctctaagct tttctaatga
    46861 tagatttaga tagtcaaaaa aatatctgaa aagttcccag gatcgtcgtc tcagcatcaa
    46921 aaatacgtga aatttgcata attaaaataa atttcagggg agt
//
LOCUS       DBDESG010000009        45365 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.009, whole genome shotgun sequence.
ACCESSION   DBDESG010000009 DBDESG010000000
VERSION     DBDESG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.009"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            26..346
                     /locus_tag="ACKB38_000481"
                     /pseudo
     CDS             26..346
                     /locus_tag="ACKB38_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            467..1411
                     /gene="manA"
                     /locus_tag="ACKB38_000482"
     CDS             467..1411
                     /gene="manA"
                     /locus_tag="ACKB38_000482"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGL7979306.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1451..2798)
                     /locus_tag="ACKB38_000483"
                     /pseudo
     CDS             complement(1451..2798)
                     /locus_tag="ACKB38_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3026..3766
                     /locus_tag="ACKB38_000484"
     CDS             3026..3766
                     /locus_tag="ACKB38_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGL7979307.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            complement(3795..4250)
                     /locus_tag="ACKB38_000485"
     CDS             complement(3795..4250)
                     /locus_tag="ACKB38_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGL7979308.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVNPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4270..5445)
                     /locus_tag="ACKB38_000486"
     CDS             complement(4270..5445)
                     /locus_tag="ACKB38_000486"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGL7979309.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5504..6349)
                     /locus_tag="ACKB38_000487"
     CDS             complement(5504..6349)
                     /locus_tag="ACKB38_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGL7979310.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6474..7031
                     /locus_tag="ACKB38_000488"
     CDS             6474..7031
                     /locus_tag="ACKB38_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGL7979311.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7050..7517
                     /locus_tag="ACKB38_000489"
     CDS             7050..7517
                     /locus_tag="ACKB38_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGL7979312.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7606..8235
                     /gene="upp"
                     /locus_tag="ACKB38_000490"
     CDS             7606..8235
                     /gene="upp"
                     /locus_tag="ACKB38_000490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGL7979313.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8409..8999
                     /gene="clpP"
                     /locus_tag="ACKB38_000491"
     CDS             8409..8999
                     /gene="clpP"
                     /locus_tag="ACKB38_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGL7979314.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9078..9326
                     /locus_tag="ACKB38_000492"
     CDS             9078..9326
                     /locus_tag="ACKB38_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGL7979315.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9427..10587
                     /locus_tag="ACKB38_000493"
     CDS             9427..10587
                     /locus_tag="ACKB38_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7979316.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10855..11724
                     /locus_tag="ACKB38_000494"
     CDS             10855..11724
                     /locus_tag="ACKB38_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGL7979317.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11728..12684
                     /locus_tag="ACKB38_000495"
     CDS             11728..12684
                     /locus_tag="ACKB38_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGL7979318.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12684..13448
                     /locus_tag="ACKB38_000496"
     CDS             12684..13448
                     /locus_tag="ACKB38_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979319.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13448..14158
                     /locus_tag="ACKB38_000497"
     CDS             13448..14158
                     /locus_tag="ACKB38_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979320.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGRIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14466..15122
                     /locus_tag="ACKB38_000498"
     CDS             14466..15122
                     /locus_tag="ACKB38_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGL7979321.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15230..16325
                     /gene="prfB"
                     /locus_tag="ACKB38_000499"
     CDS             join(15230..15301,15303..16325)
                     /gene="prfB"
                     /locus_tag="ACKB38_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGL7979322.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16343..17035
                     /gene="ftsE"
                     /locus_tag="ACKB38_000500"
     CDS             16343..17035
                     /gene="ftsE"
                     /locus_tag="ACKB38_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGL7979323.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17028..17954
                     /gene="ftsX"
                     /locus_tag="ACKB38_000501"
     CDS             17028..17954
                     /gene="ftsX"
                     /locus_tag="ACKB38_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGL7979324.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18240..20420
                     /locus_tag="ACKB38_000502"
     CDS             18240..20420
                     /locus_tag="ACKB38_000502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGL7979325.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20472..21287
                     /locus_tag="ACKB38_000503"
     CDS             20472..21287
                     /locus_tag="ACKB38_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGL7979326.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21427..22770
                     /locus_tag="ACKB38_000504"
     CDS             21427..22770
                     /locus_tag="ACKB38_000504"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGL7979327.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            22985..24175
                     /gene="metK"
                     /locus_tag="ACKB38_000505"
     CDS             22985..24175
                     /gene="metK"
                     /locus_tag="ACKB38_000505"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGL7979328.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24728..25663
                     /locus_tag="ACKB38_000506"
     CDS             24728..25663
                     /locus_tag="ACKB38_000506"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGL7979329.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25697..26734
                     /gene="holA"
                     /locus_tag="ACKB38_000507"
     CDS             25697..26734
                     /gene="holA"
                     /locus_tag="ACKB38_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGL7979330.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFSEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            26907..27512
                     /gene="sodA"
                     /locus_tag="ACKB38_000508"
     CDS             26907..27512
                     /gene="sodA"
                     /locus_tag="ACKB38_000508"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGL7979331.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27668..28180
                     /locus_tag="ACKB38_000509"
     CDS             27668..28180
                     /locus_tag="ACKB38_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGL7979332.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDDK
                     GQRHFVIRQK"
     gene            28201..29286
                     /gene="rlmN"
                     /locus_tag="ACKB38_000510"
     CDS             28201..29286
                     /gene="rlmN"
                     /locus_tag="ACKB38_000510"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGL7979333.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKECVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            29286..29768
                     /locus_tag="ACKB38_000511"
     CDS             29286..29768
                     /locus_tag="ACKB38_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGL7979334.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            29770..31311
                     /locus_tag="ACKB38_000512"
     CDS             29770..31311
                     /locus_tag="ACKB38_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGL7979335.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31367..32170)
                     /locus_tag="ACKB38_000513"
     CDS             complement(31367..32170)
                     /locus_tag="ACKB38_000513"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGL7979336.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVIFHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(32254..32467)
                     /locus_tag="ACKB38_000514"
                     /pseudo
     CDS             complement(32254..32467)
                     /locus_tag="ACKB38_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32946..33155)
                     /locus_tag="ACKB38_000515"
     CDS             complement(32946..33155)
                     /locus_tag="ACKB38_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979337.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33325..33597
                     /gene="rpsP"
                     /locus_tag="ACKB38_000516"
     CDS             33325..33597
                     /gene="rpsP"
                     /locus_tag="ACKB38_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGL7979338.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33617..33856
                     /gene="kphA"
                     /locus_tag="ACKB38_000517"
     CDS             33617..33856
                     /gene="kphA"
                     /locus_tag="ACKB38_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGL7979339.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            34044..34329
                     /locus_tag="ACKB38_000518"
                     /pseudo
     CDS             34044..34329
                     /locus_tag="ACKB38_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            34433..35221
                     /locus_tag="ACKB38_000519"
     CDS             34433..35221
                     /locus_tag="ACKB38_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGL7979340.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            35252..35770
                     /gene="rimM"
                     /locus_tag="ACKB38_000520"
     CDS             35252..35770
                     /gene="rimM"
                     /locus_tag="ACKB38_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGL7979341.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELVLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPSKRV
                     DVEILEGLDDED"
     gene            35760..36479
                     /gene="trmD"
                     /locus_tag="ACKB38_000521"
     CDS             35760..36479
                     /gene="trmD"
                     /locus_tag="ACKB38_000521"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGL7979342.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            36491..36829
                     /locus_tag="ACKB38_000522"
     CDS             36491..36829
                     /locus_tag="ACKB38_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGL7979343.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(36860..37180)
                     /locus_tag="ACKB38_000523"
     CDS             complement(36860..37180)
                     /locus_tag="ACKB38_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979344.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            37278..37493
                     /locus_tag="ACKB38_000524"
     CDS             37278..37493
                     /locus_tag="ACKB38_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGL7979345.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(37490..37636)
                     /locus_tag="ACKB38_000525"
     CDS             complement(37490..37636)
                     /locus_tag="ACKB38_000525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979346.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLTRFEEI
                     FEEY"
     gene            complement(37699..38235)
                     /locus_tag="ACKB38_000526"
     CDS             complement(37699..38235)
                     /locus_tag="ACKB38_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGL7979347.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(38432..39778)
                     /gene="gor"
                     /locus_tag="ACKB38_000527"
     CDS             complement(38432..39778)
                     /gene="gor"
                     /locus_tag="ACKB38_000527"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGL7979348.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            40043..41242
                     /locus_tag="ACKB38_000528"
     CDS             40043..41242
                     /locus_tag="ACKB38_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGL7979349.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            41226..41927
                     /locus_tag="ACKB38_000529"
     CDS             41226..41927
                     /locus_tag="ACKB38_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979350.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            41929..43188
                     /locus_tag="ACKB38_000530"
     CDS             41929..43188
                     /locus_tag="ACKB38_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979351.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            43312..45309
                     /gene="metG"
                     /locus_tag="ACKB38_000531"
     CDS             43312..45309
                     /gene="metG"
                     /locus_tag="ACKB38_000531"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGL7979352.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT        13883 a         8425 c         9889 g        13168 t
ORIGIN      
        1 tgctttttga ttttagttta gtattatcgt agaaaatgga ctcggtgcta tggatacacc
       61 ggatgagaat ggttatgtag cagatgacta tcggattact tacttagagg cccacatcaa
      121 ggccatgcga gatgccattt accaagacgg ggttgacttg cttggttata cgacttgggg
      181 ctgtatcgat ccggtttcag ctggcacagg ggaaatgaat aagcgctatg gctttatcta
      241 tgtagatcga gataatgtag gaaacggagc tctcaaacgt agcaagaaga agtccttcta
      301 ttggtacaag gatgtcattt atagcaatgg tgcaagcatc gggtagagca catttgttta
      361 ctatttttat aaaatcttct ccctgcttta taagatgtaa aaaagagttc aattcgaact
      421 ctttttttgc tataatgagg ggagaaaaat cagacaggag atcgacatgt cagaaccatt
      481 atttttacaa tcagttatgc aagaaaaaat ctggggtgga gccaagctac gtgatgagtt
      541 tggctacgac atcccaagtg aaaaaatcgg agaatattgg gccatctcag cccatccaaa
      601 tggagtctct aaagttgcca atggtcgtta cgagggaaca gatcttgcta ctttgtatgc
      661 ggaacaccgt gaattatttg gcaatcgtcc agaacctgta tttccacttt tgaccaagat
      721 cctcgatgcc aacgactggc tcagtgtcca agttcaccca gacgatgctt atggactcga
      781 gcatgaaggc gaactcggaa aaacagaatg ctggtacatt atcgcagcgg atgaaggttc
      841 agagattatc tacggtcaca atgccaagtc aaaagaagaa ctccgccagc aaatcgagga
      901 caagaactgg gatgacttgt tgaccaaagt ccctgttaag gctggagatt tcttctatgt
      961 accaagcggg actatgcacg ctatcggggc gggtatctta atccttgaaa cccagcagtc
     1021 tagcgatacc acctatcgcg tctatgactt tgaccgcaag gatgacaaag gcaacttgcg
     1081 tgaacttcat cttgaaaaat ccatcgatgt cttgaacatt ggtgagccag caaatagccg
     1141 tcctgtaact gttaaagcag atgatttgcg ttccactctc cttgtatcta atgatttctt
     1201 cgcagtttac aagtgggaaa ttactggaaa agttgacttt gaaaagacag ctgactacag
     1261 cttattgagt gtcttggctg gtcaaggtca gctaactgtt gacgggaaaa attatccaat
     1321 tcaaaaaggc agtcacttta tcctaccaag tgatgttgaa gcttggactc tggaagggca
     1381 aggtttggaa ttgattgtta gccatccata aaaagaaagg gcctgagttc actactcaag
     1441 tcctttttga ttacataaat tgggcaatga agaccacaat gatgatgatt ggaatgacga
     1501 aacgaagaaa cgaagaagga agagccagac ttggaacagc ccttgtttcc atgctctttc
     1561 atcgagatgg agttcctcca ttgcaagagc ctttttaaag atatagcctg taaaaagtga
     1621 aaggtagaga gctccaaatg gcatgaggag attggaaacc aagaagtcca tagcgtcaaa
     1681 gaaggtctta ccaaaaatgt gaacatccgc catgacaccg taagataggg ctgaaggaat
     1741 gccaaagaca aaggtcaaaa ttcctaaaat aacactccat ttggcacgtt tgctgttatc
     1801 ctggttggtg atattgtcta cattgatttc cagcatcacg acagaaaaag tgactgtcgc
     1861 aaaaaggaag agcaagagga agaggacgta gaaaatggtt ccaaaaggca tcttgtcaaa
     1921 gagttgaggc aagacgataa agagcaggct gggtccccct tcagactgga tattgaaggg
     1981 ggatcgagct tggaaaatgg ctagacctgc catgatggat atcgagatat tcatggctac
     2041 gatggagatt cctgactgga ctagattggt tttcttgtct aagtaagaag cataggtcaa
     2101 catgactgta acccctagtg agagggcaaa gaaagattgt cccagagcat agaggagacc
     2161 agtgctagtc agttttgaaa agtctggttt gaggaagtaa agaacccctt ccatggcatt
     2221 tggcaaactg agagagcgac cgatgataaa aacaaagacg ataaagagca ggggcatcat
     2281 gactttcgaa gctctttcaa tccctttttg aaccccacgt gatacaatga agatattcaa
     2341 taggataaag gccgcttgag ctcctagggc aatggctgga tttgaaatga ttgaagtaaa
     2401 taactgagca taatcacccg ttccaccaag ttggaacaat ttcccaaact caatacctag
     2461 atagactaga atccatcctc cgataacact gtaaaaagat aagaggataa agagggcaaa
     2521 ggcgccaatc caaccgataa agttgtactt gttattcttg cccagttttc caaaggtttt
     2581 gatagcggaa acgccagcac tacggccaag ggcaaactca gccagcagga gagggaaacc
     2641 gattaaaata gtggaaatga gaaagattag taaaaagcct ccaccgccat tagcagcagt
     2701 catgtaggga aacttccaaa cggaaccaag cccgatggcc agccagcaga tgctagaata
     2761 aaaccaagtt tcgaccccca ttgtgatttt tctgacatag ttaaactctg atctcctttt
     2821 tcaaaataag aaaaagctac ttgagttgtc aaatagcctt ctgtttgatt gatagacttg
     2881 actatcctat catgttttct aaggtctgtc aagaaaatca ttttcaagtt ttcacacctt
     2941 tctcaaaaaa gttaaaaaat tttctcaaaa acgcttgact ctgacctaag gcgaggggtt
     3001 atactatcat tgtaaggagg aaatcatgta ccatataaaa gaagctgcgc agctttcagg
     3061 tgtctctgtc aagaccctgc atcactatga caagatagga ctcttggtcc ccttaaagtc
     3121 ggaaaacggc tatcgaacct acagtcaaga ggatttggaa cgccttcagg tcattcttta
     3181 ctacaaatat ctaggctttt ctttagagaa aatagcagag ctgttaaagg aagaaaggac
     3241 agatttattg ccccatttga ctaggcagtt ggactatcta actcgcgaaa ggcaacatct
     3301 ggataccttg atttccacct tgcaaaaaac tattcaagaa caaaaaggag aaagaaaaat
     3361 gaccattgag gaaaaattca cgggatttag ctatcaagac aatcaaaaat accaccaaga
     3421 agcggtagag aaatatggtc aagaagtcat gggacaagcg ctcgaacgcc aaaaaggtca
     3481 cgaagacgag gctacggccg cctttaacca agtctttcaa actttggcac aaaatcttca
     3541 agttggttta cctgcaacag caaccgaaaa ccaggagcaa gcagccaagc tcttgcaagc
     3601 cattcgcact tatggatttg actgctctat tgaggtattc ggtcatatcg gtaaaggtta
     3661 cgtctacaac ccagagttta aggaaaacat tgacaagttt ggttctgaaa cagcccagta
     3721 cacgtcagat gccattgcgg tttacgttca gacaaatgca gaataaatag gctaggaatt
     3781 tcctagccta ttttttactt caaatcataa agccagtcgt caccgttttt gtagtaaaag
     3841 aattcactga gatcttcttc tagaaacaca cgaagcatat cagacatatc atcggttgca
     3901 agttttagat gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc gtctgaagac
     3961 tggagttcac cagtaaagtg ttctgtcttg taaaaaagga cgacataacg ataatccttg
     4021 tcgtcatacc agtttttgat accacagagt tggggtttgg aaatgatcag accagtttct
     4081 tctttcactt cacgaatgac agcatcgaca aaggattcgc cacgttcaac atgaccacca
     4141 ggaaaagtaa tgccagacca gtcgggatta actcggtctt ggaccaggac cttatctccg
     4201 tttttaatca tacacatgtt aacaaattcg actgcctctc ttctgttcat tcttcacaac
     4261 ctttaatctt taatcataat gcagacttcc cgccacccag ccggtacaga gggcagaagt
     4321 gatgttaaag ccacccgtgt gggcattgat atccataact tcgcctgcaa agtggaggcc
     4381 aggtaccagc ttactttcaa gggttttagg attgatttcc ttgagactga ctccaccctt
     4441 ggtaacaaag gactttgcaa gggacatttt tccagttaca ggaattttaa gttctttaat
     4501 ggactggaca agttgttctc gttccttttc agtcagttgt ttgacttttt caggatatcc
     4561 ttgtacaaaa aattcggcca agcgttctgg taacaaggtt tttaaagcgt ttttcaagga
     4621 tttttcccga ttttcttcta gaaatgtaac caagtccttc tcagaaagtt gaggcaaaac
     4681 atcgagtgag agaacctccc cacctttgac aaagctagac atgcgtaggg cagcaggacc
     4741 tgacaaacca aagtgggtaa agagtaaatc atgagtgatg acatgcttac cataacttag
     4801 ggtcacatcg tccagagaaa taccttgtaa ggctttatgt ggaaaatctg ttaataaagg
     4861 actttcagca gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa tctcgtgacc
     4921 aaaaccagtc gaaccagtcg aaggataaga cttaccacct gttgtgacaa tgagtttctc
     4981 acaagtgaag gtttgatccg ctgacttaag gacaaactgg tcatctactt ttttaacaga
     5041 aacgatttct atttgagtag caacttgacc acctagttcg gtgattttct tttccaaagc
     5101 ttcgataata gtccgagact tgtcactggc tggaaagacg cgtccgtggt cttcgacctt
     5161 aagtttaaca ccattttctg taaaaaagtt gatgatgtca tgattatcga actgggagaa
     5221 aacactgtaa agaaagcgtc cgtttccagg aattccagct agcaggttgt ctaagctacc
     5281 attgttggtc acattgcaac gtcccccacc agtcccagct aatttttttc caagtttccg
     5341 atttttttcg atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg tagccatcat
     5401 accagcaggt cccccaccga tgacaatagt atcaaaatgt ttcatagctc tattgtacca
     5461 caaaaaaaca agagatgatg gtcacctctt gtcaagaatg caattaatca atttcatagc
     5521 ccatcagcaa accgccctct tctgcataga aactgcagag accagaggtt ggtagaattt
     5581 taatatccgc ttgtgggaag gtttcacgga ttcgctctga gagctgttga caacattttt
     5641 cgttattgcg ttgggccatg acaatacggc caccagcata tccagctttt actaactcat
     5701 cataggcagc ttgaactgat ttctttgatc cccttgcttt ttgtagcaat tcgagagtcc
     5761 cagtttcact agcttttccg accatacgaa tgttgagaag gccaacgacc gtaccgataa
     5821 gcttgctcaa acggccgttc ttcaccaagt tatcgacttt ggctaggaca aagagcaact
     5881 tagttttttc ttgataggcg gtgatagctt caaccacttc ttcaaaagac aagccctggt
     5941 caatcaagtc attcaatttt tctacgagta ggtcaacttc accaccagca gataaactat
     6001 caatcacatg aatcttagtg tcaggatggt cttccagata aatattcttt gctagttgag
     6061 cactattgtg actgccagaa agggtacctg tgatggttac taggaaaatg tttttggcac
     6121 cttcaaatgc tcgcaaatag tcatctgggc ttggacaagc cgattttgaa gcttctgcag
     6181 ttgcatacat ggtttccatc atttggtcaa tatcgagact ggcgtcatca acaaagacct
     6241 gatcagctac ttgaatggtt aaggggacac ttacaaaggt tgtgttaata gctggtgttg
     6301 gcagttgacg ataatcacaa ccagagtcag caataatctt ccaagtcata gaaattctcc
     6361 atctttgtca gttatacatt gacaaaggtt ctgtcttttt ttacaattat atcatgaaaa
     6421 cccttgaaac aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat gttatgtctg
     6481 aacgtagaat ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa gaatccaatt
     6541 tattaaccag agaagccatt gaaacagccc tcttgcaact cttggaaaaa aaggaactga
     6601 ccaagattag tatttctgaa ttggtcaaac gtgcaggtgt ttcgcgtgcg gccttttatc
     6661 gcaattatga ctccaaagag gaaattttag agagcgtctt taaacgaact gtccacaata
     6721 ttatggaaca gctgcatcat tacgatttaa agacagacct ttatttggtt tgggttcacc
     6781 ttttccggga ggccagaaag gaagctagag taattcaatt ggccttggat taccatctgg
     6841 aaaaaatctt tgtccaagcc atgcaggaat ttctagaaaa ataccatggg aaatcaaaag
     6901 gtgtcagctc ttatcttcat tccttctgga gctcagccat cgtctctgtc cttctaaaat
     6961 ggatcaagga tggcatgaag gtaccagctg aaaagattgc agatttaggt ttaccatttt
     7021 ttaaaaaata gagaaaaagg agaaaagaga tgactgaaaa aagactggcc tgggatgagt
     7081 attttgcagc ccaagctcta ctaattgcga atcgttccac ttgtaaacgt gctaaagtgg
     7141 gcgcgattct ggtaaaagat aataaggtta tttccactgg ttacaatggt tcggtgtcag
     7201 ggactgagca ttgtattgat cacgaatgtt tggtcattga aggccactgc gttcgcaccc
     7261 ttcacgctga ggtcaatgct atccttcaag gtgctgagcg tggtgttccc aaaggcttta
     7321 cagcctatgt aacccatttt ccttgtctca actgtacaaa acaattgctt caggtcggct
     7381 gcaagcgcgt ggtttatatc aaccagtacc gaatggacga ctacgcccaa tacctttatc
     7441 aagaaaaagg gacagaattg acccatttac cacttgagac agtacagaca gctcttaaag
     7501 aggctgatct aatgtaaaaa ttatcaaaaa taaatggttt agaaagattt ttaaaccgtt
     7561 ttttggtata ataagaagaa taaattgaaa gaaggaattc caaaaatggg aaaaattgaa
     7621 gttattaatc acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac agatacttct
     7681 acaaaagctt ttcgtgagct agtagatgag attgcaatgt tgatggggta tgaagtactt
     7741 cgtgatcttc cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac agttcaaaaa
     7801 caattggcag gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat cgggatggtt
     7861 gatggtctct tgaacttggt tccagctgct aaagttggcc acatcggtat gtaccgtgat
     7921 gaagaaacac ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat tgaccaacgt
     7981 caaatttttg tagtagaccc aatgttggca acaggtggct cagcaatctt ggctgttgat
     8041 tctcttaaaa aacgtggcgc atcaaatatc aaatttgtct gccttgtatc tgctccagag
     8101 ggtgtaaaag cccttcaaga agctcatcca gatgtagaaa tctttacagc agccttggat
     8161 gaacgtttga acgaacacgg ttatatcgtt ccaggtcttg gagatgctgg agaccgcttg
     8221 ttcggtacaa aataagatcg aaaagaagaa tgacttggaa aagtatttcc agtcacgaaa
     8281 ggaggttggg tttttgtttc tgtctaatga aagcagagca aaaatttgac cttttttgac
     8341 caagatatta taatagtctt atcttcagtc atttgaccaa caaaattaaa actcaaaagg
     8401 agaaatgaat gattcctgta gttattgaac aaacaagccg tggagaacgt tcttacgata
     8461 tttactcacg tcttctcaaa gaccgcatca ttatgctgac aggtccggtt gaagacaata
     8521 tggctaactc tgttattgcc caattgcttt tcttggatgc ccaagatagt acaaaagata
     8581 tttaccttta tgtcaataca ccaggtggtt ctgtttcagc tggtttggca atcgtagata
     8641 ccatgaactt tatcaaggca gatgtccaaa ccattgttat gggaatggct gcatctatgg
     8701 ggactgtcat tgcatcaagt ggagcaaaag gcaaacgttt catgcttcca aatgctgaat
     8761 acatgattca ccaaccaatg ggtggtacag gtggtggtac ccaacaaact gatatggcta
     8821 tcgctgcaga acacttgctc aaaactcgta ataccttgga aaaaatcttg gctgaaaatt
     8881 caggtcagtc aatggaaaaa gtccatgcag atgcagaacg tgataactgg atgagcgccc
     8941 aggaaacact tgaatatggc tttattgatg aaattatggc caacaattca ttgaactaat
     9001 gatgatagaa ggcaaactcg actgggtttg ctttttttgg tataataggg agagatttct
     9061 tagaaagagg atttatcatg tttgaaaaag tcaatcgctc tggcttgatt atctatcttt
     9121 actataatcg tgatgccaaa aaactgcagg attatggaga tattacctat cattccaaga
     9181 aacatcgtta cttacaactc tatgttccaa ctcaagaagt ggagcaattg gtcggacgct
     9241 tgagcaagga aaaatttatt aaaaaagtta gggtttgtca tatccaagag ttggaaacac
     9301 cctttgtggg caatctttat cgataggaaa acgttatcat cgaaaaagtt caaggaaaac
     9361 attgacaatt ttctgataat tcggtatatt cttaacagac tatttaagaa ataaggagac
     9421 aaaaagatga agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt
     9481 gcagcctgtg gagaagtgaa gtctggagca gtcaacactg ctggtaactc agtagaggaa
     9541 aagacaatta aaatcgggtt taactttgaa gaatcaggtt ctttagctgc atacggaaca
     9601 gctgaacaaa aaggtgccca attggctgtt gatgaaatca atgccgcagg tggtatcgat
     9661 ggaaaacaaa tcgaagtagt cgataaagat aataagtctg aaacagctga ggctgcttca
     9721 gttacaacta accttgtaac ccaatctaaa gtatcagcag tcgtaggacc tgcgacatct
     9781 ggtgcgactg cagctgcggt agcgaacgct acaaaagcag gtgttccatt gatctcacca
     9841 agtgcgactc aagatggatt gactaaaggt caagattacc tctttattgg aactttccaa
     9901 gatagcttcc aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa
     9961 gttgttcttt acactgacaa tgccagtgac tatgctaaag ggattgcaaa atctttccgc
    10021 gagtcataca agggtgaaat cgttgcagat gaaactttcg tagcaggtga cacagacttc
    10081 caagcagccc ttacaaaaat gaaagggaaa gactttgatg ctatcgttgt tcctggttac
    10141 tataatgagg ctggtaaaat tgtaaaccaa gcgcgtggca tgggaattga caaaccaatc
    10201 gttggtggtg atggattcaa cggtgaggag tttgtacaac aagcaactgc tgaaaaagca
    10261 tcaaacatct actttatctc aggcttctca actactgtag aagtttcagc taaagctaaa
    10321 gccttccttg acgcttaccg tgctaagtac aatgaagagc cttcaacatt tgcagccttg
    10381 gcttatgatt cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa
    10441 atcaagaata accttgctaa aacaaaagat tttgaaggtg taactggtca aacaagcttc
    10501 gatgcagacc acaacacagt caaaactgct tacatgatga ccatgaacaa tggtaaagtt
    10561 gaagcagcag aagttgtaaa accataatag aaaaatgttg aaatagggaa tgagcctttg
    10621 actcactccc tgtttcgata tttaatactc ttcgaaaatc tcttcaaact gcgtcaacgt
    10681 cgccttggat tatatatgtg actgacttcg tcagtcttat ctacaacctc aaagcagtgc
    10741 tttgagcaac ctgcggctag tttcctagtt tgctctttga ttttcattga gtataagaac
    10801 ctatcaaaaa gtgagggaaa accctcggaa ttataaatag aaagagtgaa tcttatgctc
    10861 caacaactcg taaatggttt gattctaggt agtgtttacg cgctgttagc cctaggatat
    10921 accatggttt acggaattat caagctcatc aacttcgccc atggtgatat ttatatgatg
    10981 ggagccttta tcggttattt cttgatcaat tctttccaaa tgaatttctt tgtagcgctt
    11041 attgtagcta tgctagcgac agctattctt ggtgtcgtga ttgagtttct tgcttaccga
    11101 cctttgcgcc actctactcg tattgctgtt ttgattacgg ctattggggt ttctttccta
    11161 ttggagtatg gaatggtcta tctggttggt gccaataccc gtgccttccc tcaagcgatt
    11221 caaacagttc gatatgattt gggaccaatt agcttaacaa atgtgcagtt aatgattttg
    11281 gccatttcct tgattttgat gattttgtta caagtcattg tccaaaagac taagatgggg
    11341 aaagccatgc gtgcagtatc agtagatagc gacgcggcgc aattgatggg gatcaatgta
    11401 aaccgtacga ttagctttac cttcgctttg ggttctgctc ttgcgggtgc ggctggtgtt
    11461 ctgattgctc tttattataa ctctcttgag cctttgatgg gggttactcc aggtcttaaa
    11521 tctttcgttg ccgcagtact tggtggtatc ggaattattc ctggtgcggc tcttggtggc
    11581 tttgtgattg gtctattgga aacctttgcg actgcctttg ggatgtcaga tttccgtgat
    11641 gccattgttt atggaatctt gttgttgatc ttgattgtcc gcccagctgg tatccttggt
    11701 aagaatgtga aagagaaggt gtaaacgatg aaggaaaatt taaaagttaa tattctatgg
    11761 ttactccttt tgttagctgg ctatagcttg attagtgtac tggtttcagt cggagtactt
    11821 aatctattct atgtacagat tttacaacaa attggaatta atattatttt ggctgttggt
    11881 ctcaacttaa tcgttggttt ttcaggacaa ttttcacttg gtcatgctgg tttcatggcg
    11941 attggtgcct atgcagcagc tattattggt tctaaatcac caacctacgg tgccttcttt
    12001 ggagctatgc ttgtaggggc tttgctttca ggagcagttg ccttacttgt cggcattcca
    12061 accttgcgct tgaaggggga ctatcttgcg gtagcaactc tgggtgtttc tgaaattatc
    12121 cgtatcttta tcatcaatgg tggaagcctt acaaatggtg cggcaggtat cttagggatt
    12181 cctaacttta caacttggca aatggtttac ttctttgtcg tgattacaac cattgcaacc
    12241 ttgaacttct tgcgtagccc aattggtcgt tcaaccctct ctgttcgtga agatgaaatc
    12301 gctgctgagt cagttggggt taatacgact aaaattaaaa tcatcgcttt tgtctttggt
    12361 gccattactg caagtattgc tgggtcactt caggcaggat ttatcgggtc tgttgtaccg
    12421 aaagattaca ccttcatcaa ctcaatcaac gttttgatta ttgttgtatt tggtggactc
    12481 ggttccatta caggtgcgat tgtttcggct attgttctgg gaattttgaa tatgcttctc
    12541 caagatgttg ctagtgtgcg tatgattatt tacgctttgg ccttggtatt ggtaatgatt
    12601 ttcagaccag gtggactcct tggaacatgg gaactgagcc tatcacgttt ctttaaaaaa
    12661 tctaagaagg aggaacaaaa ctaatggcat tacttgaagt aaaacagtta accaaacatt
    12721 ttggtggtct aacagctgtt ggagatgtga ctcttgaatt gaacgaaggg gaactggttg
    12781 gattaatcgg tccaaacgga gctgggaaaa ccaccctttt caaccttttg accggtgttt
    12841 atgaaccaag cgagggaaca gtaaccctag atggtcacct tttgaatggg aaatcacctt
    12901 ataagattgc ctctttggga cttggacgta ctttccaaaa tatccgtctc tttaaagatt
    12961 taacagtttt agataatgtt ttgattgctt ttggaaacca tcacaaacag catgttttta
    13021 ctagtttctt acgcttacca gctttttaca agagtgaaaa agaattaaag gctaaagctt
    13081 tggaattgtt gaaaatcttt gatttagatg gtgatgcaga gactcttgct aaaaatcttt
    13141 cctacggaca acaacgtcgt ttggaaattg ttcgtgccct tgctacggaa cctaaaattc
    13201 tcttcttaga tgaaccagca gcaggtatga acccacagga aacagccgaa ttgactgagt
    13261 taattcgtcg tatcaaagat gagtttaaga ttacaatcat gttgattgaa cacgatatga
    13321 atctggtcat ggaagtaaca gaacgtatct acgtacttga atatggccgt ttaatcgctc
    13381 aaggaactcc agacgaaatt aagaccaata aacgcgttat cgaagcttat ctaggaggtg
    13441 aagcctaatg tctatgttaa aagttgaaaa tctttctgtg cattacggta tgatccaagc
    13501 agttcgtgat gtaagctttg aagttaatga aggagaagtt gtttccctta tcggtgccaa
    13561 cggtgcaggt aagacaacta ttcttcgcac cttgtcaggt ttggttcgac caagttcagg
    13621 aaagattgaa tttttaggtc aagaaatcca aaaaatgcca gctcagaaaa tcgtggcaag
    13681 tggtctttca caagttccag aaggacgcca cgtctttcct ggcttgactg ttatggaaaa
    13741 tcttgaaatg ggagctttct taaagaaaaa tcgtgaagaa aatcaagcta acttgaagaa
    13801 ggttttctca cgctttcctc gtcttgaaga acggaagaac caagatgcag ccactctttc
    13861 agggggggaa caacaaatgc ttgccatggg acgcgccctc atgtcaacac caaaacttct
    13921 tcttttagat gaaccatcaa tgggacttgc cccaatcttt atccaagaaa tttttgatat
    13981 cattcaagat attcagaagc aaggaacaac ggtcctcttg attgaacaaa atgccaataa
    14041 agcacttgca atctctgacc gaggatatgt actggaaaca gggagaatcg tcctatcagg
    14101 aacaggaaaa gaactcgctt catcagaaga agtcagaaaa gcatatctag gtggctaaaa
    14161 caatccagtg gattgtttta gtcggcagat ggagattacg aagtaatcat caatatagtc
    14221 cgggggacct ttttagtcgg tagattgaga ttgcaaacaa atctgcatct acattgaaag
    14281 cttaatttct aataattgaa aaaatcgaat gaaaaatttc ttaccttcat tcacagagct
    14341 cgatttcaga gctctttttg ctagcttatt catacttttc tgaatttcga aaaagaaatg
    14401 taagcgtttg atagatttac aaaaagattg tataataggg ataagaatag aaaaggagaa
    14461 gtctcatggc agttaaagat tttatgaccc gcaaggtagt ttatattagt ccagatacaa
    14521 cagtatctca tgcagcagat ttgatgagag agcaaggttt gcaccgtctg cctgttatcg
    14581 aaaatgatca attagttggt ttggtgactg agggaaccat tgcacaagca agtccatcta
    14641 aagcaacaag tctttctatc tatgagatga attatcttct gaataagaca aaagtaaaag
    14701 atgtcatgat tcgcgatgtt gtcactgtct caggctatgc tagtctagaa gatgcaactt
    14761 atctgatgtt gaaaaataag attagtattc tccctgtcgt agataaccat caagtatacg
    14821 gagttattac tgaccgtgac gttttccaag cctttcttga aattgcaggt tatggcgaag
    14881 aagggattcg tgtacgcttt gttacagaag atgaagttgg tgttcttgga aaaattgttt
    14941 ctttgattgt agaagaaaat ttgaatatct cccatacagt caatattccg cgtaaggatg
    15001 gtaaggtgat tatcgaagtg caaatcgatg gatcaattga tttaccagcc ttgaaagaaa
    15061 aatttgaagc aaatggtatt caagtggaag aaatcgctcg cacttcagca aaagtcttgt
    15121 aagaagggaa gcccaaaggc ttcttttttc atgaaaaggg gattagagca aaagatggaa
    15181 agaaatgata aaatatgcta taatgaaata atgtaaaaaa ggagtattta tggacatttc
    15241 agtaattcgt cagaaaattg acgcaaatcg tgaaaaatta gcttctttca gggggtctct
    15301 ttgacctcga agggctagag gaagagattg ccatcttgga aaacaagatg acagaacctg
    15361 atttttggaa cgataatatt gcggcccaaa aaacgtcgca agaattaaat gaattaaaaa
    15421 acacttacaa taccttccat aagatggaag agttgcagga tgaagtcgaa attttattgg
    15481 attttttggc tgaagacgag tcagtgcatg atgaactggt agcgcagtta gccgaacttg
    15541 ataagataat gaccagctac gagatgactc tactcttgtc agaaccttat gaccacaaca
    15601 atgccatctt ggaaatccat ccaggttctg gtggtactga ggcgcaggac tggggtgata
    15661 tgttgcttcg tatgtatact cgttatggta atgctaaagg ctttaaagtg gaagtgttgg
    15721 attaccaagc aggtgatgag gctggtatta agtcggtaac tttatcattt gaagggccta
    15781 atgcctatgg tctcctcaag tcagaaatgg gtgttcaccg cttagtgcga atctcaccat
    15841 ttgactctgc caaacgtcgc catacctctt tcacatctgt agaagtgatg ccagaattgg
    15901 atgatactat tgaagtggaa atccgtgaag atgatatcaa gatggatacc ttccgttcag
    15961 gtggtgccgg tggacaaaac gtcaataagg tttcaacagg tgtacgttta acccacattc
    16021 caactggaat tgttgtccaa tcaacagtag atcgtaccca gtatggaaat agagatcgtg
    16081 ccatgaagat gttgcaggct aagctctatc aaatggagca agataagaag gctgcggagg
    16141 tagattctct caaaggtgag aaaaaggaga tcacttgggg aagccaaatc cgttcttatg
    16201 tcttcacgcc ttatactatg gtaaaagatc accgaactag ctttgaggtt gctcaggtag
    16261 ataaggttat ggatggggac ctagatggtt ttatcgatgc ttatctcaag tggcgaatta
    16321 gctaagatag aaaggaactc acatgtcaat tattgaaatg agagatgtcg ttaaaaaata
    16381 cgacaacgga acaactgctc tacgcggtgt ttcggttagc gttcaaccgg gggaatttgc
    16441 ttacatcgta ggaccttcag gagcagggaa gtcaactttt attcgttctc tgtatcgtga
    16501 agtaaaaatc gataaaggaa gcctatcagt tgctggtttt aatctggtta agatcaaaaa
    16561 gaaagatgtc ccgcttctac gtcgtagtgt tggggttgtc ttccaggatt ataaattgtt
    16621 accaaagaaa actgtctatg aaaatattgc ttacgctatg gaagtaatcg gggaaaatcg
    16681 ccgtaatatc aaaagacgag tgatggaagt tttggacttg gttggattga agcataaggt
    16741 tcgttctttc ccaaatgaac tctcaggtgg ggagcaacag cggattgcga ttgcgcgtgc
    16801 aattgtaaat aatcccaaag tattgatagc tgatgagcca acaggaaatc tggatccgga
    16861 taattcatgg gaaattatga atctcttgga acggattaac ctacaaggaa caactatttt
    16921 gatggcgact cataatagcc agattgtaaa taccttgcgc caccgtgtca ttgccattga
    16981 aaatggccgt gtcgttcgtg acgaatcaaa aggagagtat ggatacgatg attagtagat
    17041 tttttcgcca tttatttgaa gccttaaaaa gtttgaaacg aaatggttgg atgacagtag
    17101 ctgctgtcag ttcagtcatg attactttga ccttggtggc aatatttgca tctgttattt
    17161 tcaatacagc gaaactagct acagatattg aaaataatgt ccgtgtagta gtttatatcc
    17221 gaaaggatgt ggaagataat agtcagacaa ttgaaaaaga aggtcaaact gttacaaata
    17281 atgactacca caaggtatat gattctttga agaacatgtc tacggttaaa agtgttacct
    17341 tttcaagtaa agaagaacaa tatgaaaaat taaccgagat aatgggagat aactggaaaa
    17401 tctttgaagg agatgccaat cctctctatg atgcctatat tgtagaggca aacactccaa
    17461 atgatgtaaa aactatagcc gaagatgcta aaaaaattga aggtgtctct gaggttcaag
    17521 atggcggtgc caatacagaa agactcttca agttagcttc atttatccgt gtttggggac
    17581 tagggattgc tgctttgtta atttttatcg cagttttctt gatttcaaat accattcgta
    17641 ttaccattat ttcccgcagt cgcgaaattc aaatcatgcg cttggtcgga gctaaaaaca
    17701 gttatatccg tggaccgttc ttgttagaag gagcctttat cggtttattg ggagctatcg
    17761 caccatctgt tttggtcttt attgtttatc aaattgttta ccaatctgtc aacaaatcgt
    17821 tggtagggca aaatctatcc atgattagtc cagatttatt tagtccgttg atgattgccc
    17881 tactatttgt gattggggtt ttcattggtt cattgggatc aggaatatcc atgcgccgat
    17941 tcttgaagat ttaggtaaaa tagctgcttt tatgaggaga ttgtaaaatc tccttttttg
    18001 ctacaagagt ttttgaaaag agatgcgcag aagaaaagag cttccaaaga agtcccccag
    18061 agaagacttc ttgatggcta atttgaactt gaaatttggc gcactagaaa ttttttagaa
    18121 aaaagtgtaa tttttttata aaagccttta caaaaaaaat taaagtggta taattaaatc
    18181 atagaaagtg caaacgtttt cgtaaaacgt ttacctaaat aaaaataaag gagatttgaa
    18241 tgatgaaaga tacattcaaa aatgtcttgt ctttcgaatt ttggcaaaaa ttcggtaagg
    18301 ctttgatggt agttatcgcg gttatgccgg ctgctggttt gatgatttca atcggtaagt
    18361 ctatcgtgat gattaaccca acctttgcac cacttgtcat cacaggtgga attcttgagc
    18421 aaatcggttg gggggttatc ggtaaccttc acattttgtt tgccctagcc attggaggaa
    18481 gctgggctaa agaacgtgct ggtggtgctt tcgccgctgg tcttgccttc atcttgatta
    18541 accgtatcac tggtacaatc tttggtgtat caggcgatat gttgaaaaat ccagatgcta
    18601 tggtaactac tttctttggt ggttcaatca aagttgctga ttactttatc agtgttcttg
    18661 aagctccagc cttgaacatg ggggtattcg tagggattat ctcaggtttt gtaggggcaa
    18721 ctgcttacaa caaatactac aacttccgta aacttcctga tgcactttca ttcttcaacg
    18781 ggaaacgttt cgtaccattt gtagttattc ttcgttcagc aatcgctgca attctacttg
    18841 ctgctttctg gccagtagtt caaacaggta tcaataactt cggtatctgg attgccaact
    18901 cacaagaaac tgctccaatt cttgcaccat tcttgtatgg tactttggaa cgtttgctct
    18961 tgccatttgg tcttcaccac atgttgacta tcccaatgaa ctacacagct cttggtggta
    19021 cttatgacat tttaactggt gcagctaaag gtactcaagt attcggtcaa gacccactat
    19081 ggcttgcatg ggtaacagac cttgtaaacc ttaaaggtac tgatgctagt caatatcaac
    19141 acttgttaga tacagtacat ccagctcgtt tcaaagttgg acaaatgatc ggttcattcg
    19201 gtatcttgat gggtgtgatt gttgctatct accgtaatgt tgatgctgac aagaaacata
    19261 aatacaaagg tatgatgatt gcaacagctc ttgcaacatt cttgacaggg gttactgaac
    19321 caatcgaata catgttcatg ttcatcgcaa cacctatgta tcttgtttac tcacttgttc
    19381 aaggtgctgc cttcgctatg gctgacgtcg taaacctacg tatgcactca ttcggttcaa
    19441 tcgagttctt gactcgtaca cctattgcaa tcagtgctgg tattggtatg gatatcgtta
    19501 acttcgtttg ggtaactgtt ctctttgctg taatcatgta ctttatcgca aacttcatga
    19561 ttcaaaaatt caactacgca actccagggc gcaacggaaa ctacgaaact gctgaaggtt
    19621 cagaagaaac cagcagcgaa gtgaaagttg cagcaggctc tcaagctgta aacattatca
    19681 accttcttgg tggacgtgta aacatcgttg atgttgatgc atgtatgact cgtcttcgtg
    19741 taactgttaa agatgcagat aaagtaggaa atgcagagca atggaaagca gaaggagcta
    19801 tgggtcttgt catgaaagga caaggggttc aagctatcta cggtccaaaa gctgacattt
    19861 tgaaatctga tatccaagat atccttgatt caggtgaaat cattcctgaa actcttccaa
    19921 gccaaatgac tgaagcacaa caaaacactg ttcacttcaa agatcttact gaggaagttt
    19981 actcagtagc agacggtcaa gttgttgctt tggaacaagt aaaggatcca gtatttgctc
    20041 aaaaaatgat gggtgatgga tttgcagtag aacctgcaaa tggaaacatt gtatctccag
    20101 tttcaggtac tgtgtcaagc atcttcccaa caaaacatgc ttttggtatt gtgacggaag
    20161 caggtcttga agtattggtt cacattggtt tggacacagt aagtcttgaa ggtaaaccat
    20221 ttacagttca tgttgctgaa ggacaaaaag ttgcagcagg agatctcctt gtcacagctg
    20281 acttggatgc tatccgtgca gcaggacgtg aaacttcaac agtagttgtc ttcacaaatg
    20341 gtgatgcaat taaatcagtt aagttagaaa aaacaggttc tcttgcagct aaaacagcag
    20401 ttgctaaagt agaattgtaa tatacttgag gttggaagct gtattccaac ctcttatttt
    20461 gggagaaaag aatgaaattt ttaacactca atactcacag ttggatggag aaagaagcag
    20521 aggaaaaatt ccagattttg cttgaagata ttcttgaaaa ggactatgat ttgatttgtt
    20581 ttcaagaaat caatcaggag atgacctcgt cagaggtgga ggttaatgac ctttatcaag
    20641 ctttgccagc agctgagcct attcaccaag accattatgt tagactcttg gttgaaaagt
    20701 tgtctgagca agggaaaaat tactactgga cctgggccta taaccatatc ggctataacc
    20761 gctaccacga aggtgtggct atcttgtcta aaacacctat tgaagccaga gaaattttgg
    20821 tttcagatgt ggatgatcca acagactatc atactcgccg tgttgcccta gctgaaactg
    20881 tagtcgatgg caaggagcta gcagttgcca gtgttcatct ctcttggtgg gataaaggtt
    20941 tccaagaaga atgggcacga tttgaggctg tcttgaaaaa attgaacaag ccacttttac
    21001 tagctggaga tttcaacaat ccggctggac aggaaggtta ccaagctatt ttagctagtc
    21061 cattaggctt acaagacgca tttgaagttg ctcaagagaa aagtggtagc tatactgttc
    21121 cgcctgaaat tgatggctgg aaagggaaca ctgaacccct tcgaatcgat tatgtcttta
    21181 ctaccaaaga gttagcggtg gaaaatttac atgtcgtatt tgatggtaac aagagtccac
    21241 aagtgagtga tcactatggc ttgaatgcta tattaaactg gaaataataa ctgaaaagag
    21301 gttggaacta taaaattcca gccttttctt actagagaag ctactggaaa tagcctaaat
    21361 aagtgagact actgtaatgg aataaaatat ggtataattg ataaggtaga tagaatcgag
    21421 gatgttatgt catttacgaa atttcaattt aaaaactata ttagagaagc cttgaaggag
    21481 ttaaaattta caactccaac agaggtgcaa gacaagttga ttcctattgt tttggcaggt
    21541 cgtgacctag taggagaatc aaaaacaggt tcaggtaaga ctcatacttt cttgttaccg
    21601 attttccagc aattagatga agctagcgat agtgtacaag cagtgattac tgcaccgagt
    21661 cgtgagttgg ctactcaaat ttaccaagta gcgcgtcaga tttcagctca ctcagatgtc
    21721 gaagttcgtg tggttaatta tgtgggtggt acggataagg ctcgccagat tgagaaattg
    21781 gcaagcaatc agcctcatat tgttattgga acaccaggcc gtatctacga cttggttaaa
    21841 tctggtgatt tagctattca taaagccaag acatttgttg ttgatgaagc agatatgacc
    21901 ttggatatgg gattcttgga aactgttgat aagattgctg gcagtcttcc aaaagacttg
    21961 caattcatgg tcttctcagc gactatccca caaaaactgc aaccattctt gaaaaaatac
    22021 ttatcaaatc ctgttatgga gaaaattaag accaaaacgg ttatttctga caccattgat
    22081 aattggttga tttcgaccaa gggacatgat aagaatgctc aaatttacca gttgactcag
    22141 ttgatgcagc cgtatttggc aatgattttt gttaacacta aaacgcgtgc tgatgaattg
    22201 cattcatatc tgactgctca aggcttgaag gttgcaaaaa tccatggcga tattgcccct
    22261 cgtgaacgca agcgaatcat gaatcaggtg caaaatctgg attttgagta tattgtcgca
    22321 acagatttgg cagcgcgtgg gattgacatt gaaggtgtca gccatgtcat caatgatgcc
    22381 attccgcaag acttatcttt ttttgttcat cgtgttggtc gtactggacg aaatggccta
    22441 ccaggtacag ctattaccct ttatcagcca agtgatgact cggatatccg tgagttggag
    22501 aaattgggaa tcaagtttag tcctaagatg gtcaaagacg gggaatttca agatacctat
    22561 gaccgtgatc gtcgtgccaa ccgtgagaaa aaacaagata aacttgatat cgaaatgatt
    22621 ggtttggtta aaaagaaaaa gaaaaaagtc aaaccgggtt ataagaagaa aattcaatgg
    22681 gcggttgatg aaaagcgccg taaaaccaag cgtgctgaaa atcgcgctcg cggtcgtgca
    22741 gagcgtaaag ctaaacgcca aacattttaa tagaaattgt tggagtattg agctccaact
    22801 tttttattta tgagaacgaa ctatctaaac cgaaacacta cattaaagac tgcaaattgc
    22861 gattaaaaat ggtataatga taaagttata tagtcccgat aagatggtag gtatttatta
    22921 cgaagagttt tcctatcagt actttgtaac tctataacaa tattttttaa ggggggacat
    22981 ttttatgtca gagcgtaaat tattcacgtc tgaatctgta tctgaggggc atccggataa
    23041 gattgcagac caaatttcag atgcgatttt ggatgctatt ttagcaaagg atccagaggc
    23101 gcacgttgct gctgaaacag ctgtatatac tggttctgtc cacgtttttg gtgaaatttc
    23161 tacaaatgcc tatgtggata ttaaccgtgt ggttcgtgat accattgcag agattggtta
    23221 taccaataca gaatatggat tttctgctga gacggtggga gtacacccat ctttggtgga
    23281 acaatctcct gacatcgctc aaggtgttaa cgaagccttg gaggttcgtg gaaatgctga
    23341 tcaagatcca ctggacttga ttggagcagg tgaccaaggg ctcatgtttg gatttgcagt
    23401 agatgaaaca gaagagctta tgccattgcc aattgcactc agtcataaat tggttcgtcg
    23461 tctggcagaa cttcgtaagt ctggagaaat tagctatctc cgtccagatg caaaatcaca
    23521 agttacagtt gagtacgatg aaaatgaccg tccggtacgt gtagatacag tcgttatttc
    23581 tactcagcat gatccagagg ccactaatga acaaatccat caagatgtga ttgacaaggt
    23641 catcaaagaa gttattccat cttcttatct tgatgataag acaaaattct ttatcaatcc
    23701 gacaggtcgt tttgtaatcg gtggtcctca aggggactca ggtttgactg gtcgtaagat
    23761 tattgtagat acttatggtg gctactctcg tcatggtggt ggtgccttct ctggtaaaga
    23821 tgcgactaag gtggatcgtt cagcctctta tgcggctcgc tatattgcca agaatatcgt
    23881 tgcagcagac cttgctaaga aggcagaagt gcagttggcc tatgctatcg gtgttgcgca
    23941 acctgtttct gttcgtatcg atactttcgg tacaggaaca gtagctgaaa gtcaacttga
    24001 aaaagcggct cgtcaaatct ttgaccttcg ccctgcaggg attatccaaa tgctggacct
    24061 caagcgtcca atttaccgtc aaacatcggc ttacggtcac atgggacgta cagatattga
    24121 tcttccatgg gaacgtttgg ataaggtaga tgctttgaaa gaagcagtaa aataagattt
    24181 taagagggga acgtcctctc ttttttatag tttttaacta tactgggata ctgttctgaa
    24241 aatccatttt gcgaaagtag agatttacat gtatagtaga ttgaaactag aatagtacac
    24301 ctcaacttct aaaacattgt tagcaatcaa tttgactgtc ctgatcgatt tctcctgttc
    24361 ttgtttcatt ttactatatt tctttaaaaa tgataaaggt taagatttct cctcgtaata
    24421 gataatcttg gggatatttc aatccaaagt tttattcgtt atcacttgac tattgcaagg
    24481 ttttctagag caacagagtc atggaatgga ctcatggttg agatttctcc ttgttgcttg
    24541 gacttcattc aaaagtctgt tacccaagcc ttgttcaaac ttctaataca ctagctgttt
    24601 ccatagcatg acttctgtac tagactttct tttccgaata aatagataga accacagaat
    24661 ctagtaaacc tagaattaaa attatggtat aatattagca ataaaagaaa tctggaggat
    24721 tagaatcatg gtatcaacga aaacacaaat tgctggtttt gagtttgaca attgcttgat
    24781 gaatgcagca ggtgtggctt gtatgacgat agaggagtta gaagaggtca aaaactcagc
    24841 ggcaggaacc tttgttacta agacagcgac cttggacttc cgtcagggga atcctgagcc
    24901 acgctaccaa gatgttccac ttggttccat caactctatg ggcttgccaa ataatggctt
    24961 agactattat ttggattatc ttttagattt gcaggaaaaa gagtcgaacc gaactttctt
    25021 cttatctctg gtcggcatgt ctccagagga aacccatact attttgaaaa aagtccaaga
    25081 gagtgatttt cgtggtctga ctgagctaaa tctttcctgt ccaaatgttc caggtaaacc
    25141 tcagattgcc tatgattttg agacaacaga ccggattttg gcagaagtgt ttgcttactt
    25201 caccaaacct cttggaatta aattgccacc ttattttgat attgttcact ttgaccaagc
    25261 ggcagctatt ttcaacaaat atccgctcaa gtttgtcaac tgcgttaact ctatcggaaa
    25321 cggcctctat atagaagacg aatctgtcgt tattcggcct aagaatggtt ttggtggaat
    25381 tggtggagaa tacatcaaac cgactgcttt agccaatgtt cacgcctttt atcaacgttt
    25441 aaatcctcaa atccaaatta tcggaacagg tggcgttctg actggtcgag atgcctttga
    25501 acacatcctc tgtggagcaa gtatggtgca ggtgggaacg acccttcaca aagaaggcgt
    25561 cagtgctttt gaccgcatta ccaatgaact gaaagcaatc atggtggaaa aaggctacga
    25621 gagcttagaa gatttccgtg ggaaattgcg ctatattgac taaattaaat cgaaaaatct
    25681 gaagaaagga gagacgatgc tagccattga agaaagtcag aagttgactt tatcaaattt
    25741 accgagcctg agcctattta cagggacaga tcagggtcag tttgaagtga tgaagagtca
    25801 aatgttgaaa cagattgggt atgattctgc tgacctcaac tttgcctact ttgatatgaa
    25861 agaagtagtt tacaaggatg tggaactgga gttggtcagc cttcctttct ttgcggatga
    25921 gaaaatcgtg atattagatt attttatgga tatcacgact gctaagaaac gctttttgac
    25981 agatgatgag cttaagtcat ttgaggaata ccttgacaat ccttctccaa caaccaagtt
    26041 gataatcttt tcagaaggaa agctggatag caaaagacgg ttagtcaaat tacttaagcg
    26101 tgatgccaag gccttcgatg cagtagaagt aaaagaacaa gaattgcgcc agtacttcca
    26161 aaagtggagt cagaaacaag gtctgcagtt taccaatcat tcttttgaaa atctcctcat
    26221 caagtcgggg tttcaattta gcgaaatcca gaaaaatctt ctctttttac agtcctataa
    26281 ggcgaattct gttattgagg aagaggatat tgttaacgca attcccaaga ccttgcagga
    26341 caatattttt gatttaactc agtttattct gactaaaaag atggatcagg cgcgcgattt
    26401 ggtgagagac ttgaccttgc aaggggaaga tgaaatcaaa ctgattgcag tcatgctggg
    26461 acaatttcgg acttttactc aggtgaagat tttggcggag tctggccaaa cagaatcgca
    26521 gattgcaagt agtttaggta gttatctggg acgtaaccca aatccttatc aaatcaagtt
    26581 tgcattaaga gattcgagag gactttcttt gagctttttg aagcaagcta tttcctattt
    26641 gattgagaca gactatcaga ttaagacagg tctttatgaa aaaggtttcc tttttgaaaa
    26701 ggcactctta cagattgcta gtcaggtcaa ttgacatttg ttgaaactac aacccgcgtc
    26761 aggactatct tgtcgtgggt ttcttgctga tttgcttatt ttattagaaa gtatgtcagt
    26821 gatttcctta tctataagca aaaaacttga aaaaagtgaa tgtttgcgtt atcattttca
    26881 tatagaaaga aaaagaggta ttacagatgg ctattatctt accagaactt ccatatgcat
    26941 acgacgcttt ggaaccatac atcgatgcgg aaacaatgca tttgcaccat gacaaacacc
    27001 atcaaactta tgtcaacaat gccaatgcag ctttagaaaa acaccctgaa atcggtgaag
    27061 accttgaagc cttgcttgct gatgtagaat ctatcccagc tgatatccgt caagcactta
    27121 tcaacaatgg tggcggacac ttgaaccacg ctcttttctg ggaattgatg actcccgaga
    27181 aaacagctcc ttcagcagaa ctggcagcag caatcgatgc aacatttggt tcatttgaag
    27241 aattccaagc agccttcact gcagcagcaa caactcgttt tggttcaggt tgggcatggt
    27301 tggttgtcaa caaagaaggg aaacttgaag tgacttcaac agcaaaccaa gacacaccaa
    27361 tctcagaagg taaaaaacca atcttgggct tggacgtttg ggaacatgct tactacgtga
    27421 aataccgcaa cgtgcgtcct gactacatca aagctttctt ttcagtaatc aactggaata
    27481 aagtagatga attgtacgca gctgctaaat aatgatagtt ggagggaaga attgttcttc
    27541 tctttttagg ttataagatt ctggtctgac aaaatcgtca gacttttttc atttttatga
    27601 gaaacgtgct aactgctaga aattatggta gaataaagta ttaagtaatc gtgaaaaaag
    27661 gatatctatg cgaaaagaaa ttgcacctga attatacaac tataacaagt ttcctggtcc
    27721 ggagttccat ttacacgggg acaaggtcga aacggaaggg atagcttttt ccttggttga
    27781 aaatatcaag gatgcctttg atgtgacgac ttttaatcag cgtttttcag aagtattaac
    27841 caagtttgat tatatcgtgg gggactggag caacgaacag cttcgcctac gaggttttta
    27901 caaggatgac cgaacagaag aaaaacttga aaaaatcagt cgtttacaag actacctttt
    27961 agagtattgt agttatggtt gtgcctattt tgtcttagaa aatgaagccc ctaagcgagc
    28021 atcatttgac aagaaaatgc gtaagacgga agaagaaaca ccttctagaa aaggaaagaa
    28081 accgactcaa actaaacgaa agtcgaatgc agataagaaa aatagacgtc gtcagaaaga
    28141 cgacaaggga caacgtcatt ttgtcattcg tcagaagtga gtagagaata aggagaagaa
    28201 atgaaaccct caattcatag tttggtacat caaaccatgc aggaatgggt attggagcag
    28261 ggagaaaaga aattccgtgc agatcaaatc tgggaatggc tctaccgtaa acgtgtgcag
    28321 tcatttgaag aaatgaccaa cctttccaag gatttgattg ctaagctcaa tgaccagttt
    28381 gtggtcaatc ccttgaaaca acgtattgtg caagagtctg ctgatggtac agtcaaatat
    28441 ctctttgaat tgcccgacgg tatgttaatt gagactgtac tcatgcgtca acactatggt
    28501 ttatctgtct gtgtgaccac tcaggtcggc tgtaatatcg gttgtacctt ctgtgcctct
    28561 ggtttgatta agaagcaacg tgacctcaat aacggtgaaa tcgtagcgca aatcatgctg
    28621 gttcagaaat actttgatga gcgtggtcag gatgaacgca tcagccatat cgttgtcatg
    28681 ggaatcggtg agccctttga taattacaac aatgtcttga atttctttcg tactatcaat
    28741 gatgataaag gaatggctat cggtgctcgt cacatcacgg tttcaacctc aggtttggct
    28801 cataaaattc gtgactttgc tgatgaaggg gttcaggtca atcttgccgt gtctcttcac
    28861 gcacctaaca atgaacttcg ttcaagtatc atgaagatta accgtgcctt tccgattgaa
    28921 aaactctttg ctgctattga atactacatt gagacaacaa accgtcgtgt aacctttgaa
    28981 tatatcatgc ttaatgaagt caacgacggt gtagaacaag ccttggaatt gactgaattg
    29041 ctcaagaaca tcaagaaatt gtcttatgta aacttgattc cttataaccc agttagtgag
    29101 catgaccaat atagccgtag tcccaaagag tgcgtgttgg ctttctatga tacgcttaag
    29161 aaaaaagggg tcaactgtgt ggttcgtcaa gagcatggta cagatattga cgcagcttgt
    29221 ggacaattgc gctctaacac aatgaaacgt gaccgccaga aggcagtcgc agcggtaaat
    29281 ccttaatgat gaagaagact tataatcata ttttggtctg gggagtcatt ttctatagca
    29341 tttgcattgt ctgtttttgc tttactcctc aagaacaatc taccgtggga gtgggaactc
    29401 caggtattca gcatcttgga cgcctggttt ttcttttgac tcctttcaat tctctctgga
    29461 aactgggcga agtgagtgac attggacaat tatgttggat ttttttacaa aatatcctca
    29521 atgtcttctt gttttttcct ctgattttcc aactccttta tctatttcca aatttgcgga
    29581 aaacaaaaaa ggtccttctt tttagttttc ttgtgagtct tggaatcgag tgtacgcaat
    29641 taatcttgga ctttttcttt gatttcaatc gcgtctttga gattgatgat ttgtggacca
    29701 acactttggg tggctatctg gcttggctcc tttataaacg attacataaa aacaaggtaa
    29761 ggaattaaaa tgagtatttt agaagttaaa aatctgagtc acggttttgg tgaccgtgca
    29821 atttttgaag atgtgtcctt ccgtctcctc aagggagaac atatcggcct ggtcggtgcc
    29881 aatggtgaag gaaaatcaac ctttatgagt atcgtgactg gtaaaatgct gccagatgaa
    29941 ggaaaggttg agtggtccaa atatgtgacg gctggttact tggatcagca ctctgtcctt
    30001 gctgaaagac agtcggtgcg tgatgttctc cgtacggctt ttgatgagct tttcaaagct
    30061 gaagctcgta tcaatgacct ctatatgaaa atggctgaag acggcgcgga tgttgatgct
    30121 ctcatggaag aagtaggaga acttcaagac cgtctggaga gtcgtgattt ctataccttg
    30181 gatgctaaga ttgacgaagt agcgcgtgct cttggtgtta tggactttgg catggatacg
    30241 gatgtaactt ctttgtcagg tgggcaaaga accaaggtgc ttttggcaaa acttctcctt
    30301 gaaaagcctg atatcttgct gttggacgag ccgaccaact acttggatgc tgagcatatt
    30361 gattggctca agcgctatct ccaaaactat gagaatgcct ttgttctcat ttcgcacgat
    30421 attccattcc tcaatgacgt tattaatatt gtctatcatg tggaaaatca acagctgacg
    30481 cgttactctg gtgactacta ccagttccaa gaagtttatg ctatgaagaa atctcagcta
    30541 gaggcagcct acgaacgcca gcagaaagag attgcagacc tcaaggactt tgtggctcgt
    30601 aataaagccc gtgttgcaac tcgtaatatg gctatgtctc gtcaaaagaa attggataag
    30661 atggatatta tcgaactcca aagtgagaaa ccaaaaccat cctttgattt caaaccagct
    30721 cgtacaccag ggcgctttat cttccaagcc aagaacttgc aaattggtta cgaccgtcct
    30781 cttactaagc ctttaaatct taccttcgaa cgcaatcaaa aggttgcgat tattggtgct
    30841 aatggtattg gaaaaacaac tctcttgaag agtctcttgg gcattatctc gccaatcgct
    30901 ggggaagtgg agcgtggaga ttatttagaa cttggttatt ttgagcagga agtagaaggc
    30961 ggtaatcgcc aaactcctct tgaagctgtc tggaatgcct ttcctgccct taatcaagca
    31021 gaagtccgtg cagcccttgc ccgttgtggt ttgacaacca aacatattga aagccagatt
    31081 caagtattat cagggggaga gcaagccaag gttcgtttct gtctcttgat gaatcgtgaa
    31141 aacaacgttt tagtgctgga cgagccgacc aaccatttgg atgtggatgc aaaggatgag
    31201 ctcaaacgcg ctctcaaaga atatagggga tctatcctta tggtctgcca cgagccagac
    31261 ttttatgaag gctggataga ccaaatatgg gattttaata atttaactta agaatgagag
    31321 taaaagaaat acagtaccga atgtggatac tgtatttttt tggtttttaa gatttaaaat
    31381 cgtagtcttt caccacttcg atgctgtcga ttgtgatagc agtagttggc ttgtcttttt
    31441 catctttttc ggccttagca atcttatcca caacatccat accgtcaatc acttgaccaa
    31501 agactgggtg tttgccatct agactagggt ttccaccttc tttgtaggct tcaataattt
    31561 tctgtggata cttgcttgta gggagtttag aagaggtatc tgtagagttt tggttgatga
    31621 agaactggct gccattggtg tttggttgac cagtattagc catagcaaga gcaccacgga
    31681 tgttatacaa ataaggagta atctcgttct tgaaaccagt tcctttgtct ttagtcttat
    31741 ccttgtcatg ccagatggac tgaccacctg taccgtcccc ttttggatct ccagtttgga
    31801 ccataaagcc atcgatgaca cggtggaagg taataccgtt atagtagcct tctttggcgt
    31861 gagtgaggaa attttcaacc gctagaggag cgagtttagg gaagagttta atgcgaatat
    31921 caccttggct tgtgtggaaa atcacttcgg cttcatcttc agcaacttcc ttagatagtt
    31981 gtgggaaatt ggcgttttcg tttgttaaag catcgtttaa ctccttggca gattgggcag
    32041 ctactttgga actttcttca gcagcaagac tggaatcaac ataatcatca ccacgcagac
    32101 tgcgttggac gctgctacac ccagctaggg ctacagtaga cagtaaaaga agggttgcta
    32161 gttttttcat tctgatcctc tcaaaaattc atttataacc attgtaccct aaaaggaagt
    32221 ggatttcaaa tattagtagt ttacagaact ggcttagata atagttggga tttcatcatc
    32281 atcttgaaaa tcttcaactt cttccccagt aaatggtttc tgaaaagaac ttagtataaa
    32341 acgggcaatt ggttcaacaa ttaggaaatt tagaggaaga gctgcaataa agtttacgaa
    32401 ccaggcgctt agataggtat tgggtgtgat agcaatgcca ttgtaaaaaa agtccataga
    32461 aggacatgaa agtgaccagc taattcttct tctgaaaaat gctcatgaag ggcttgatcc
    32521 aggtcgcaag atttcatttt agaaagtcca gtaactccct tcttcttcag gatattcttt
    32581 ttcatggtat tatttagatg gtcaagtgag tcaaaagaag tttcgacaag agcataccct
    32641 ttttctacca gagtttttag atgtttaggg gcatcaatac cataagtgta ctcaaagtat
    32701 tttggaaacc agatcttaag aaagctcgta aagctagcca gttctctaaa cgctaagaac
    32761 tagctacaac acagcattta caacacttca tggttcacac catggggtgt tttttaatga
    32821 ttttaagcaa aattcacact taattgaaag ttatttaaat tagatgaaaa tttatttttt
    32881 gaaaaagtgg gaaaacattg attctaagcg aattttgaat catattcgaa tgggagtttt
    32941 taaaatcatg tttcagtggt aaaagtacca aactggattc tccatagtaa aatgatatct
    33001 cctgctagtg aatcatctac taataagtcc atatttcgct tgggaacggg cttgggactt
    33061 agaagccaag tatccaactc tcgttcaggc gatatgaagg gtttgacttg gtgttcttta
    33121 taaaattttt ctaaaatagt attcacggat agcatctttc aaacatttat ttcctattct
    33181 aacaaatttc taaatacttt aagaaattta tagtctgtca agtttttttc ttgacacatg
    33241 ccagaaatct gctataatag aacatgtgct aaatagctca gctatttcac cgaaataaaa
    33301 aattaaagaa atgagacttt aaaaatggca gttaaaatcc gtttgactcg tatgggttct
    33361 aagaaaaaac ctttctaccg tatcaacgta gcagattcac gttcaccacg tgacggacgt
    33421 ttcatcgaaa cagttggaac ttacaaccca cttgttgctg aaaaccaagt aactttgaaa
    33481 gaagaccgcg ttcttgcatg gttggctaat ggagctcaac cttcagacac agtacgtaac
    33541 atcctttcaa aagaaggcgt attgaaaaaa ttccacgatt ctaaattttc aaaataagtt
    33601 taaagtaggt tgacagatgg atacgattga aaatctcatt attgcgattg tgaaaccctt
    33661 aatttcacaa ccagatgcct taactatcaa gattgaggat acaccagaat ttttggaata
    33721 tcatttgaat cttgatcaaa gcgatgtggg tcgtgtaatc ggtcgtaagg gtcgcactat
    33781 ttctgcgata agaacgattg tctactctgt cccaactgaa tacaaaaaag taagaatcgt
    33841 tattgacgaa aaataagaag ggcgggacgg atgtctcgct tttttgtgtg gaatagaaga
    33901 tatagtaaaa tgaaacaaga acaggacaaa tcgatcagga cagtcaaatc gatttctaac
    33961 aatgttttag aagtaaaggt gtactattct agtttcaata tactataaaa gattgaacgt
    34021 ttaggcaatt ccaagactac ttaatcgaac attaacagca gtctcggact gatgaaggtc
    34081 tctttatgaa gttagtggag gaagtcggag aagtagtcga ggttttgaat aggcgctctg
    34141 gtcgaaaaga agggatagga ttcgaacgag gaacttgcca gagaactggc tgatatcatt
    34201 tactacaccg tcgcaatcgt agctattaat catattgacc ttaccaagac tgtctttgag
    34261 aaagacaaaa ccgcagccat taattaccaa cataaacata atttggaagg attattgaag
    34321 ggaaagtaga gatattgtcc ccttcctcca acttgagaaa atccttggaa cgtgataaaa
    34381 taaaggataa ggatttttaa taattcatta tatagaatca gtggatttct ttatgaagag
    34441 gtttgaagta tctacagaaa ttggtagcct ctctgttact tatcaaaagc aaaagaaagt
    34501 gctagtttgt ttaaacggag caggtttgct accaagttat gaaaattttt cacttatact
    34561 tgaaaaacct cctcctacaa ttggttattt gacaattgat tttccgaaca caggtaggag
    34621 tccgattcat gaccaagctg gaaaaaatct agataatctt gcagatgcgg tttatgaagt
    34681 acttgaagaa ttaggaattt ctgaatatat actttgtgca catagttgga gtggaatttt
    34741 agcttgcaaa ttactcgaga aaccaattaa gcgtcagact ttagtagcaa ttgaaccgac
    34801 aactaaaaaa gtcatgtttg ctgatttttc agaaaatcct tatccagaaa tggaagagca
    34861 gatgaggctg attgacgagt gtggtcctga actttatttt aagaacttaa ctcaagcaac
    34921 atttagtcct gaaacgaata aaaaaatctg ggaattaatg caagaaaaag gcttagagtt
    34981 ggaaaatcaa gatccagaat ttcagatatc tggagagatt actgaggaag attttgagga
    35041 tgtatctata gaagctcata ttcctgtatt tgttttttgt cagccttata gagaaaaaga
    35101 gtacagagaa tcagaatatt ggacttccaa tactaaactc attttaggag ggaatcacca
    35161 ttatttacaa tggtcagaat cggaaaaaat tgcggctatt attcgagaat tgttagaata
    35221 agatggaaaa aaggagatta caggagacaa gatgaactac tttaatgttg ggaaaatcgt
    35281 taatacgcag ggattacagg gggagatgcg agtcttgtct gtgacggatt ttgcagaaga
    35341 acggtttaaa aaaggagctg agctggtttt gtttgatgaa aaagatcagt ttgtccaaac
    35401 agtgaccatc gctagccacc gtaaacagaa gaactttgac attattaaat tcaaagatat
    35461 gtaccatatc aatactatcg aaaagtacaa gggatacagt ctcaaggtcg ctgaggaaga
    35521 tttgaatgac ctagacgatg gtgaatttta ctatcacgag attatcggtt tggaagtcta
    35581 tgagggtgat agcttggttg gaaccatcaa ggaaatcctg caaccaggtg ctaatgatgt
    35641 ctgggtggtc aaacgaaaag gcaaacgtga tttgctttta ccttatatcc caccagtggt
    35701 tctcaatgtt gatattccaa gtaaacgggt cgatgtggaa atcttagaag ggttagacga
    35761 tgaagattga tattttaacc ctctttccag agatgttttc tccactggag cactcaatcg
    35821 ttggaaaggc tcgagaaaaa gggctcttgg atatccagta tcataatttt cgagaaaatg
    35881 ctgaaaaggc ccgtcatgta gatgatgagc cctacggagg cggtcagggc atgttgctca
    35941 gagcacaacc tattttcgat tcctttgatg ctattgaaaa gaaaaatccg cgcgttattc
    36001 tccttgatcc tgctggaaag cagtttgatc aggcttatgc tgaagatttg gctcaagagg
    36061 aagagctaat ctttatctgt gggcactatg agggttatga tgagcgcatt aagaccttgg
    36121 taacagatga gatttcccta ggtgactatg tcctcactgg tggagaattg gcagctatga
    36181 ccatgattga tgctacagtt cgcctgattc cagaagtgat tggcaaggag tctagccacc
    36241 aagatgatag tttttcttca ggtcttttag aatatcctca gtacacacgt ccctatgatt
    36301 atcgaggcat ggtcgtgcca gatgtattga tgagtggcca ccatgaaaag attcgtcagt
    36361 ggcgattgta cgagagttta aagaaaacct acgagcgcag accggattta cttgaacatt
    36421 atcaactgac agtagaagaa gaaaaaatgc tggcagaaat caaagaaaac aaagaataaa
    36481 ggagaaacct atgcaagtaa tcaaacgtaa tggcgaaatt gctgaattta atccagataa
    36541 gatttaccaa gccatcttga aggcagccca aactgtttat gttttgacag atgatttgcg
    36601 tcaaaatctt gctcaagtca ctaagaaggt agttttggat ttacaagaag ccaaggtgga
    36661 acgtgcgact atcagtatga ttcaatctat ggttgaacat cgtttattgg gcgcaggtta
    36721 cattaccatt gcagaacact acatttccta tcgtttacaa cgtgacttgg aaagaagtgg
    36781 ttatggagat catatcgcag ttcatttaca ttttgaacaa attcgctaag aaaaaagagt
    36841 gggatgaagc aactacatct cactctttct taaatctatt tttttgggct gtttggacag
    36901 ttgaggggac aaatcgcagg cgttgaatat cgcttatacg gataatccgg ctcacatttt
    36961 tggaaaaatt tttcacgata atttgctggc cttgctgatc atatttgatg atgtcacctg
    37021 taaagcttgt ctcagacaag ataaggtgaa cagcggtttg tttctggata gcctcttcaa
    37081 tagtggctgt aagggagttg gtttcagtct ggtcaggttg gtcatatcca tttaaaaaat
    37141 catgtatttt atttagaact tgctggaatt tatgtctcat attggcctcc cttcttttat
    37201 atcaatatta tataaaattt tcctaaaatc tacaagattt tacgaataaa ctaatgaaac
    37261 taaaaaagga gaagaaaatg gcagaattta catttgaaat cgaagagcac ttgttgactc
    37321 tttctgaaaa cgaaaaaggt tggaccaagg aaattaaccg tgtgagcttt aatggtgccc
    37381 ctgcaaagtt tgatattcgt gcttggagtc cagaccatac taaaatgggc aaagggatta
    37441 ctctctcaaa tgaagaattt caaacgatgg tggatgcctt taaaggcaac taatactctt
    37501 cgaaaatctc ttcaaaccgc gtcagctttg ccttgccgta tgtatggtta ctgacttcgt
    37561 cagtttcatc cacaacctca aaacagtgtt ttgagcagcc tgcggctagc ttcctagttt
    37621 gctctttgat tttcattgag tataagtttt caaaataaaa gaaaaggata ttgagtcatg
    37681 ataactcgat atcctttttt agttagtaaa gtaagcctga tttttaaggc gttgaagtag
    37741 gggacggcta ataaaactaa tagctagaag tttgccaagg tctggaatga taaagggaag
    37801 aacccccaca gcaagagctt tttcaaatgc cattccagct aggaaatgca agctgagaat
    37861 cccgccgaca aagacaaggg catcacccaa gaggtttgca agaaaaatct taacaacacc
    37921 actcttgctg ttggttagag aggaagtaag tccagagtaa acgagataaa accaaagata
    37981 gcctgcagta gggccaacta aagcctgaaa accagctcca cctcctgcaa agacaggaag
    38041 accgatagca cctagaagaa gatagagtcc agcagaaagt acagcctctc tcggtctaaa
    38101 gacagtagca atcaagccga ttgcaaagtt ttgcagagtg aagggaacag gtccaattgg
    38161 aagactgatt tgtgccaaag cagcaatgag agcagcccca atagcaggga tagcataaac
    38221 gtgagctttt ttcaaaatag ttaacctctt ttctaatgta tagtaactat tatactcata
    38281 taggagtcga tgtcaaccat tttttaaaaa caaggtaaca aaaactgtaa cagccgattt
    38341 tcgtgactag tcaagttgga taattttttt gttgattcga aaatcattaa aaaagaagca
    38401 gacatattta tatcagcttc ttttgtttcg attaacgcat ggttacaaat tcttcagatg
    38461 cagttgggtg aattgccaca gttgcatcaa agtcagcctt ggttgctccc attttgatag
    38521 caacggcaaa tccctgaatc atttcatcaa cgccgtagcc aattccatga agtccgacaa
    38581 ctttttcttc tgaaccagct gttatgagtt tgaaacggga ttcttgacgg ttgcgagtgc
    38641 aagcagagta catagatgca aagcttgatt tataaacctt gatttggtct tgaccgtatt
    38701 ctttaatagc ttgctcttct gtcaaaccaa cagttccgat agcagggtgt gaaaagacaa
    38761 cagttggaat agttgagtaa tccatttttg cagtagtttt tccgttaaag agacgttcag
    38821 atagggtacg tccggccttg attgcaactg gagtcagttc tttctcgccc gttacatcac
    38881 ctagagcata gattccctca acaacagtat tttggtattc atccacttgg ataaagccac
    38941 gttcgttcag agtcactcca gctttttcaa gttgcaagcc cttaacgttt ggacggcgac
    39001 ctgtagccca gataacttgg ctagctgtgt gactagtacc atcttcgaaa tgaatggtaa
    39061 tgccgtcagt agttttttct aacttgacag ggactttgtg agtgtgaagt ggtaagtttg
    39121 ttctttccat ttccttgacc aaaccttcaa cgatgtagga atcaaaacca cgtaaaggac
    39181 gatcgcggcg aacaaagaga tctgtcttga caccaaaagt gtggagtacg ccagctaatt
    39241 caacggcgat ataaccagcg cctagaatgg caattgactc tggaagttct tcccaggcaa
    39301 atacatcatc agaagagcca cctagctcag caccaggaat attaggaata cttggatggg
    39361 cacctgtagc aatcacgata tgtttagcac gaatcagttc accatttacg cttacagtat
    39421 gagaatctac aaattcagca tgaccttcaa tcaagtctac accgttgcgt ttaaaactac
    39481 catcataaga agaacgagcg cgatcaatgt aggcttcacg attgcgacgt agggttgcaa
    39541 agtcaaagtt aagatcagta gtcttaaagc cgtagtcttc tccaaattga tggaaagtct
    39601 cagcgatttg cgccccgtac cacatgattt ttttaggaac acaaccgacg ttgacacagg
    39661 ttccacctaa tttcttttcc tcaataacgg ctgctttggc tccatgttca ccagcacggt
    39721 tcatggtagc gattcctccg ctacctccac cgatagcaat gatatcatat tctctcattg
    39781 aaaactcctt tgtaatcttt taaatagtag agcgtctttt ttgatagtta tattttattc
    39841 tttttttgat ttgattgcaa aaatctgcta cgcacaaaaa attgcaaaaa agcttgccaa
    39901 ataagtcaag atagtgtatt atatatctag tacaatagaa aagcaaaatt ccgaacgatt
    39961 gtttaatcct gaactgtatt gtttgctgga gggaaatttg gtataatagc aaagtattgt
    40021 aaaaaagaaa aggagtttca ttatgaagaa aaagaatggt aaagctaaaa agtggcaact
    40081 gtatgcagca atcggtgctg cgagtgtagt tgtattgggt gctgggggga ttttactctt
    40141 tagacaacct tctcagactg ctctaaaaga tgagcctact catcttgttg ttgccaagga
    40201 aggaagcgtg gcctcctctg ttttattgtc agggacagta acagcaaaaa atgaacaata
    40261 tgtttatttt gatgctagta agggtgattt agatgaaatc cttgtttctg tgggcgataa
    40321 ggtcagcgaa gggcaggctt tagtcaagta cagtagttca gaagcgcagg cggcctatga
    40381 ttcagctagt cgagcagtag ctagggcaga tcgtcatatc aatgaactca atcaagcacg
    40441 aaatgaagcc gcttcagctc cggctccaca gttaccagcg ccagtaggag gagaagatgc
    40501 aacggtgcaa agcccaactc cagtggctgg aaattctgtt gcttctattg acgctcaatt
    40561 gggtgatgcc cgtgatgcgc gtgcagatgc tgcggcgcaa ttaagcaagg ctcaaagtca
    40621 attggatgca acaactgttc tcagtaccct agagggaact gtggtcgaag tcaatagcaa
    40681 tgtttctaaa tctccaacag gggcgagtca agttatggtt catattgtca gcaatgaaaa
    40741 tttacaagtc aagggagaat tgtctgagta caatctagcc aacctttctg taggtcaaga
    40801 agtaagcttt acttctaaag tgtatcctga taaaaaatgg actgggaaat taagctatat
    40861 ttctgactat cctaaaaaca atggtgaagc agctagtcca gcagccggga ataatacagg
    40921 ttctaaatac ccttatacta ttgatgtgac aggcgaggtt ggtgatttga aacaaggttt
    40981 ttctgtcaac attgaggtta aaagcaaaac taaggctatt cttgttcctg ttagcagtct
    41041 agtaatggat gatagtaaaa attatgtctg gattgtggat gaacaacaaa aggctaaaaa
    41101 agttgaggtt tcattgggaa atgctgacgc agaaaatcaa gaaatcactt ctggtttaac
    41161 gaacggtgct aaggtcatca gtaatccaac atcttccttg gaagaaggaa aagaggtgaa
    41221 ggctgatgaa gcaactaatt agtctaaaaa atatcttcag aagttaccgt aatggtgacc
    41281 aagaactgca ggttctcaaa aatatcaatc tagaagtgaa tgagggtgaa tttgtagcca
    41341 tcatgggacc atctgggtct ggtaagtcca ctctgatgaa tacgattggc atgttggata
    41401 caccaaccag tggagaatat tatcttgaag gtcaagaagt ggctgggctt ggtgaaaaac
    41461 aactagctaa ggtccgtaac caacaaatcg gttttgtctt tcagcagttc tttcttctat
    41521 cgaagctcaa tgctctgcaa aatgtagaat tgcccttgat ttacgcagga gtttcgtctt
    41581 caaaacgtcg caagttggct gaggaatatt tagacaaggt tgaattgaca gaacgtagtc
    41641 accatttacc ttcagaatta tctggtggtc aaaagcaacg tgtagccatt gcgcgtgcct
    41701 tggtaaacaa tccttctatt atcctagcgg atgaaccgac aggagccttg gataccaaaa
    41761 caggtaacca aattatgcaa ttattggttg atttgaataa agaaggaaaa accattatca
    41821 tggtaacgca tgagcctgag attgctgcct atgccaaacg tcagattgtc attcgggatg
    41881 gggtcatttc gtctgacagt gctcagttag gaaaggagga aaactaagat gcagaatctg
    41941 aaatttgcct tttcatctat catggctcac aagatgcgtt ctttgcttac tatgattggg
    42001 attattatcg gtgtttcatc agttgttgtg attatggctt tgggtgattc cctatctcgt
    42061 caagtcaata aagatatgac taaatctcag aaaaatatta gcgtcttttt ctctcctaaa
    42121 aaaagtaaag acgggtcttt tactcagaaa caatcagctt ttacggtttc tggaaaggaa
    42181 gaggaagttc ctgttgaacc gccaaaaccg caagaatcct gggtccaaga ggcagctaaa
    42241 ctgaagggag tggatagtta ctatgtaacc aattcaacga atgccatctt gacctatcaa
    42301 gataaaaagg ttgagaatgc taatttgaca ggtggaaaca gaacttacat ggacgctgtt
    42361 aagaatgaaa ttattgcagg tcgtagtctg agagagcaag atttcaaaga gtttgcaagt
    42421 gtcattttgc tagatgagga attgtccatt agtttatttg aatctcctca agaggctatt
    42481 aacaaggttg tagaagtcaa tggatttagt taccgggtca ttggggttta tactagtccg
    42541 gaggctaaaa gatcaaaaat atatgggttt ggtggcttgc ctattactac caatatctcc
    42601 cttgctgcga attttaatgt agatgaaata gctaatattg tctttcgagt gaatgatacc
    42661 agtttaaccc caactctggg tccagaactg gcacgaaaaa tgacagagct tgcaggctta
    42721 caacagggag aataccaggt ggcagatgag tccgttgtat ttgcagaaat tcaacaatcg
    42781 tttagtttta tgacgacgat tattagttcc atcgcaggga tttctctctt tgttggagga
    42841 actggtgtca tgaacatcat gctggtttcg gtgacagagc gcactcgtga gattggtctt
    42901 cgtaaggctt tgggtgcaac acgtgccaat attttaattc agtttttgat tgaatccatg
    42961 attttgacct tgttaggtgg cttaattggc ttgacaattg caagtggttt aactgcctta
    43021 gcaggtttgt tactgcaagg tttaatagaa ggtatagaag ttggagtatc aatcccagtc
    43081 gccctattta gtcttgcagt ttcggctagt gttggtatga tttttggagt cttgccagcc
    43141 aacaaggcat cgaaacttga tccaattgaa gcccttcgtt atgaatgaga tcaacaagat
    43201 ggacactcgt ctatcttgtt tttgtatgga tttccctatt gaaattcatt aaaaatgtga
    43261 tataatgaag tgttagaaaa acaggtttca tttgcctgga aaggaaaaat tatgtctgaa
    43321 aaaaattttt atattacaac gccgatttac tatccatctg ggaaacttca tatcggttct
    43381 gcctacacaa ctatcgcatg tgatgtccta gcacgttaca aacgcctgat gggctacgat
    43441 gtattttatc tgacaggtct tgatgaacat ggtcagaaaa tccagcagaa agcggaagaa
    43501 gctggtatta cacctcaagc ctatgttgat ggaatggcgg ttggagttaa agaactctgg
    43561 caattactag atatctcata cgataaattt atccgtacaa ctgatgacta ccatgaaaaa
    43621 gttgtcgcac aagtctttga acgcttactt gctcaagatg atatctactt gggtgaatac
    43681 tctggttggt attcagtatc agacgaagaa ttctttacag aaagccagct ggcagaagtt
    43741 ttccgtgatg aagctggaaa tgtgactggt ggtattgctc catcaggtca tgaggttgaa
    43801 tgggtttctg aagaatcata cttccttcgc cttagcaaat accaagaccg tttggtcgaa
    43861 tttttcaaag ctcatcctga atttatcacg ccagatggtc gccttaatga aatgctacgc
    43921 aacttcatcg agccaggctt ggaagatttg gcggtatctc gtacaacctt tacatgggga
    43981 gtgcctgtcc catcaaatcc aaaacacgtt gtctacgttt ggattgatgc ccttcttaac
    44041 tatgcgacag ctcttggcta cgctcaagac gaacatggta actttgacaa gttctggaat
    44101 ggaacagtct tccatatggt aggaaaagat atccttcgct tccactctat ctactggcca
    44161 atccttctta tgatgttgga tgttaaatta cctgatcgtt tgattgccca tggttggttt
    44221 gtcatgaaag acggaaaaat gtctaagtca aaagggaatg tcgtttaccc tgaaatgttg
    44281 gtagagcgtt atggactaga tccacttcgt tactacctca tgcgtaacct tccagttggt
    44341 tcagacggaa cctttactcc tgaagactat gtcggccgta tcaactatga attggctaat
    44401 gaccttggga acctccttaa ccgtacggtt tccatgatta ataagtactt tgatggacaa
    44461 atccctgcct atgtagaagg tgtgactgaa tttgatcatg ttcttgctga ggttgcagaa
    44521 caatcaatcg cagacttcca tacacacatg gaagcagttg actatccacg tgcgcttgaa
    44581 gcagtctgga ctctgatctc tcgtaccaat aaatacatcg atgagaccgc accatgggtc
    44641 ttggccaagg atgaagctct tcgtgaccaa ttggcaagtg tcatgagcca cttggcagcc
    44701 agcattcgtg tagttgctca cttgattgaa ccatttatga tggaaactag tcgtgcagtt
    44761 ttgactcaac ttggtttgga agaagtttct agtcttgaaa acttgagttt ggctgacttc
    44821 ccagcagatg tgactgtagt tgccaaagga acacctatct ttccacgtct aaatatggaa
    44881 gaagaaatcg cctatatcaa ggaacaaatg gaaggcaata aaccagcagt cgaaaaagaa
    44941 tggaatccgg acgaagttga gctcaaacta aacaaggatg aaatcaagtt tgaagacttt
    45001 gacaaggttg aaatccgtgt cgcagaagtc aaagaagtgt ctaaagtaga aggttcagat
    45061 aagttgcttc aattccgctt ggatgctggt gatggagaag atcgtcagat tctttcagga
    45121 attgcaaaat actatccaaa tgaacaagaa ttggtcggca agaaagttca aatcgttgct
    45181 aacctcaaac cacgtaaaat gatgaaaaaa tatgtcagcc agggtatgat tctctcagct
    45241 gaacatgatg gcaaattaac ccttctcaca gttgatccag ctgtaccaaa tggaagtgtg
    45301 attgggtaaa agtagacagg actagttcag gctagttctg tttttttatt tatactcaat
    45361 gaaaa
//
LOCUS       DBDESG010000010        43697 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.010, whole genome shotgun sequence.
ACCESSION   DBDESG010000010 DBDESG010000000
VERSION     DBDESG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 43697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.010"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..309)
                     /locus_tag="ACKB38_000532"
     CDS             complement(<1..309)
                     /locus_tag="ACKB38_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HGL7979353.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGGKVLAVRDLY
                     G"
     gene            complement(467..1189)
                     /locus_tag="ACKB38_000533"
     CDS             complement(467..1189)
                     /locus_tag="ACKB38_000533"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979354.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            complement(1376..2142)
                     /locus_tag="ACKB38_000534"
                     /pseudo
     CDS             complement(1376..2142)
                     /locus_tag="ACKB38_000534"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            complement(2314..4272)
                     /locus_tag="ACKB38_000535"
     CDS             complement(2314..4272)
                     /locus_tag="ACKB38_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7979355.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(4475..6097)
                     /locus_tag="ACKB38_000536"
     CDS             complement(4475..6097)
                     /locus_tag="ACKB38_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGL7979356.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRIFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            6203..7648
                     /locus_tag="ACKB38_000537"
     CDS             6203..7648
                     /locus_tag="ACKB38_000537"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGL7979357.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMEKSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(7691..7888)
                     /locus_tag="ACKB38_000538"
     CDS             complement(7691..7888)
                     /locus_tag="ACKB38_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGL7979358.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(7989..8638)
                     /locus_tag="ACKB38_000539"
                     /pseudo
     CDS             complement(7989..8638)
                     /locus_tag="ACKB38_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(8622..9134)
                     /locus_tag="ACKB38_000540"
     CDS             complement(8622..9134)
                     /locus_tag="ACKB38_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGL7979359.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(9220..10155)
                     /locus_tag="ACKB38_000541"
     CDS             complement(9220..10155)
                     /locus_tag="ACKB38_000541"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGL7979360.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(10226..10525)
                     /locus_tag="ACKB38_000542"
     CDS             complement(10226..10525)
                     /locus_tag="ACKB38_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGL7979361.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(10515..11264)
                     /locus_tag="ACKB38_000543"
     CDS             complement(10515..11264)
                     /locus_tag="ACKB38_000543"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGL7979362.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(11318..11677)
                     /locus_tag="ACKB38_000544"
     CDS             complement(11318..11677)
                     /locus_tag="ACKB38_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGL7979363.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(11679..13079)
                     /locus_tag="ACKB38_000545"
     CDS             complement(11679..13079)
                     /locus_tag="ACKB38_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGL7979364.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(13374..13613)
                     /gene="rpsR"
                     /locus_tag="ACKB38_000546"
     CDS             complement(13374..13613)
                     /gene="rpsR"
                     /locus_tag="ACKB38_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGL7979365.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(13645..14115)
                     /gene="ssbA"
                     /locus_tag="ACKB38_000547"
     CDS             complement(13645..14115)
                     /gene="ssbA"
                     /locus_tag="ACKB38_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGL7979366.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(14127..14417)
                     /gene="rpsF"
                     /locus_tag="ACKB38_000548"
     CDS             complement(14127..14417)
                     /gene="rpsF"
                     /locus_tag="ACKB38_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGL7979367.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(14570..15916)
                     /gene="asnS"
                     /locus_tag="ACKB38_000549"
     CDS             complement(14570..15916)
                     /gene="asnS"
                     /locus_tag="ACKB38_000549"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGL7979368.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(15932..16297)
                     /locus_tag="ACKB38_000550"
                     /pseudo
     CDS             complement(15932..16297)
                     /locus_tag="ACKB38_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(16290..17477)
                     /locus_tag="ACKB38_000551"
     CDS             complement(16290..17477)
                     /locus_tag="ACKB38_000551"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGL7979369.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(17474..17962)
                     /locus_tag="ACKB38_000552"
     CDS             complement(17474..17962)
                     /locus_tag="ACKB38_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGL7979370.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            18283..18831
                     /locus_tag="ACKB38_000553"
     CDS             18283..18831
                     /locus_tag="ACKB38_000553"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGL7979371.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(19108..19836)
                     /gene="yaaA"
                     /locus_tag="ACKB38_000554"
     CDS             complement(19108..19836)
                     /gene="yaaA"
                     /locus_tag="ACKB38_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGL7979372.1"
                     /translation="MKILIPTAKEMNTDFPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(19915..20079)
                     /locus_tag="ACKB38_000555"
     CDS             complement(19915..20079)
                     /locus_tag="ACKB38_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGL7979373.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(20146..21741)
                     /locus_tag="ACKB38_000556"
     CDS             complement(20146..21741)
                     /locus_tag="ACKB38_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979374.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            complement(21753..22163)
                     /locus_tag="ACKB38_000557"
     CDS             complement(21753..22163)
                     /locus_tag="ACKB38_000557"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGL7979375.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(22279..23070)
                     /gene="yghU"
                     /locus_tag="ACKB38_000558"
     CDS             complement(22279..23070)
                     /gene="yghU"
                     /locus_tag="ACKB38_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGL7979376.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(23083..25779)
                     /locus_tag="ACKB38_000559"
     CDS             complement(23083..25779)
                     /locus_tag="ACKB38_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGL7979377.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIRTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            26083..27267
                     /gene="mntE"
                     /locus_tag="ACKB38_000560"
     CDS             26083..27267
                     /gene="mntE"
                     /locus_tag="ACKB38_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGL7979378.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSNALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(27410..29281)
                     /locus_tag="ACKB38_000561"
     CDS             complement(27410..29281)
                     /locus_tag="ACKB38_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGL7979379.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(29278..30477)
                     /locus_tag="ACKB38_000562"
     CDS             complement(29278..30477)
                     /locus_tag="ACKB38_000562"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGL7979380.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAI
                     TEKYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            complement(30474..31241)
                     /gene="dapB"
                     /locus_tag="ACKB38_000563"
     CDS             complement(30474..31241)
                     /gene="dapB"
                     /locus_tag="ACKB38_000563"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGL7979381.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(31518..32366)
                     /locus_tag="ACKB38_000564"
     CDS             complement(31518..32366)
                     /locus_tag="ACKB38_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGL7979382.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(32368..32742)
                     /locus_tag="ACKB38_000565"
     CDS             complement(32368..32742)
                     /locus_tag="ACKB38_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGL7979383.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(32816..34168)
                     /gene="glmM"
                     /locus_tag="ACKB38_000566"
     CDS             complement(32816..34168)
                     /gene="glmM"
                     /locus_tag="ACKB38_000566"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGL7979384.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVNYYVDTITDVVRAEIGID
                     "
     gene            complement(34192..34971)
                     /locus_tag="ACKB38_000567"
     CDS             complement(34192..34971)
                     /locus_tag="ACKB38_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGL7979385.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(34958..35815)
                     /gene="cdaA"
                     /locus_tag="ACKB38_000568"
     CDS             complement(34958..35815)
                     /gene="cdaA"
                     /locus_tag="ACKB38_000568"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGL7979386.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            35951..36916
                     /locus_tag="ACKB38_000569"
     CDS             35951..36916
                     /locus_tag="ACKB38_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGL7979387.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(36950..37861)
                     /gene="whiA"
                     /locus_tag="ACKB38_000570"
     CDS             complement(36950..37861)
                     /gene="whiA"
                     /locus_tag="ACKB38_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGL7979388.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(37858..38835)
                     /locus_tag="ACKB38_000571"
     CDS             complement(37858..38835)
                     /locus_tag="ACKB38_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGL7979389.1"
                     /translation="MRKPKITVIGGGTGSPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(38832..39722)
                     /gene="rapZ"
                     /locus_tag="ACKB38_000572"
     CDS             complement(38832..39722)
                     /gene="rapZ"
                     /locus_tag="ACKB38_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGL7979390.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(39774..40154)
                     /locus_tag="ACKB38_000573"
     CDS             complement(39774..40154)
                     /locus_tag="ACKB38_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGL7979391.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(40165..40752)
                     /gene="yihA"
                     /locus_tag="ACKB38_000574"
     CDS             complement(40165..40752)
                     /gene="yihA"
                     /locus_tag="ACKB38_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGL7979392.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(40761..41993)
                     /gene="clpX"
                     /locus_tag="ACKB38_000575"
     CDS             complement(40761..41993)
                     /gene="clpX"
                     /locus_tag="ACKB38_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGL7979393.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(42025..42195)
                     /locus_tag="ACKB38_000576"
     CDS             complement(42025..42195)
                     /locus_tag="ACKB38_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979394.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(42195..42701)
                     /locus_tag="ACKB38_000577"
     CDS             complement(42195..42701)
                     /locus_tag="ACKB38_000577"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGL7979395.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(42831..43349)
                     /locus_tag="ACKB38_000578"
     CDS             complement(42831..43349)
                     /locus_tag="ACKB38_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGL7979396.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
BASE COUNT        12708 a        10005 c         8235 g        12749 t
ORIGIN      
        1 accgtaaaga tcacgcactg ccaagacctt acctcctact ggaaagacgc taaaggccag
       61 tacaatagct gacatccctg agctagtcgc tagggcatag tctgctgact caatagccgc
      121 caagacttcc tcagccttac tacgagttgg atttttagtg cgcgtatagt caaacccagt
      181 agatcgacca aactctggat gctgataggt cgttgaaaaa tgaagtggtg tcaccaaagc
      241 acctgttgcc tcatcagact tgatccctgc ttgtgctaaa attgtgttaa tgtgtaattc
      301 cttgctcata caattcctcc aaatctatag taactattgt accacttatt ttgtatcctt
      361 cgttttcttg ttttcaagag ctagttatag tttcaaacta tataaaaagg gagtttttcc
      421 tgctcccttt aatagactat aaaatggtga atctcaaaag acaccttcac tctatcattt
      481 gctcctgcac aaaacgagca taacgctcat gattttccag tagttcctta tgagttcctg
      541 agccagtgat tttcccctcc tctaagaaga aaatacaatc cacatctttt accgttgaca
      601 aacgatgcgc tataatcaca accgtcttct cctttagtac agaatagagg ctactgataa
      661 tcgcatactc agaatccgca tcaagattag cagtggcttc atcaaatata agaatttcag
      721 catcttttaa gtaggctcta gctatttgaa gtctttggct tcgcccccct gacaagagtc
      781 gtccgcgttc accaacttca gtatctagtc cctctttcat ggagcgaatc tcatcaccta
      841 gtgatactaa gtctagcact ttcatcaatt catcatcagt tactaagcga ttcaaaccga
      901 gacaaagatt gtcacgaata ctgccagata agactgcatt attttgtgaa acccaagcga
      961 ttttacttct ccattctttt aagttaaaat catatatact tgattgctcc attagaatat
     1021 ctcctgaaag cggtttataa aaccgctcta acaaacgcac aatcgttgat tttcctgatc
     1081 cagatggtcc aacaaaagca attttttgcc ccttgaaaat tgaacaagta atatccttta
     1141 agacaggtcg attttcatca taaccaaaat agacatggtt aaaattcaac cctcgtcctg
     1201 ataccgattt tcctccctca aatttttctt taggaactgc aagcaagttc tccagtgcaa
     1261 ctgaagatcc cttgctccta gaataaacag ttacaaaatt agctatatta ctaataggat
     1321 taagtaattg aaagaggtaa atcaaaaacg aaaccaaggt tcccacagat atatatcctg
     1381 cgctgacccg ataaccccca taggttagca tcacagctat agtcgcaaag ataaataaga
     1441 gagcaaacgg ggtctcaaaa gaagtaaccc tatctgattt cagtgaattg ttttgtaccc
     1501 tttcaataca attatccaaa acatcctgta cacttttctc tgcttggtta gtcttaatta
     1561 attcatgttc ttgaatcttt tcagtcaatt gccctgttaa atttcctcct gtaaacgacg
     1621 actatacttt tcactgatat tggaaagggg caagataata aacatcatac aaggaagagt
     1681 gatgaataaa agtagagaaa gattccaatc aagactaaat aagactacaa tggaaccaag
     1741 taccataact aaactcagaa taatatttgg gaaagtcgta attaaaaact cacgaatgac
     1801 actcgtgtca ttgacaatgg cagaagtcaa ctccccactt tggctcttat caaagaagga
     1861 tttctctaca taaatcaacc cctctatcac ttttttcctg atttttgcta tctttttttc
     1921 acccgattga ctaaacagat agtaaccaat agaagaaaac aaggcttgac caataaaaat
     1981 caaaaacgat tgaaatactt tggagcctat attttcaata gaactcccat ctattaaatc
     2041 ctttaagata aggggaagca acaaagcaag tagactagac agaacaagta agaaactccc
     2101 cataatcacc ttagtatcta ctcttaataa ttttaatttc ataaatactc cttataatat
     2161 ttcaacggat aaagtcggga ataactcaat ttgaggataa aatctaataa atcttcctat
     2221 aacaaaacgc ataacatcta ggattttata tacctgatat tatgcgtttt taagcacaaa
     2281 gacttcttac acaaacttat ctacaattag attttatttg acatgttttg ccaattcttc
     2341 ttgggctttt ttattggatt cttctttttc tttcaaccat ttttctctgg cttttgcata
     2401 ttcgtctgtt gtgacaatct tatcttgtac tttgaggtat ttatatgatt caaccccttt
     2461 tgtaccggtt aaaccatagg cagcagcaaa tggtacggtt cttctcaatg atggtgttcc
     2521 cccacgcgaa acacttggaa gaactaaaga actatcaatc aaccaagctt gaatatcagc
     2581 atatttctca taacgtttgg ccggatcttg ctctttatta gcttcttcca acatttgagt
     2641 atagacatcc agtccaactg ccttagcctt gtcattggcc tcaccaggct ctagtccaag
     2701 attttgcaga aatcctccac tattagtatt aaaaatatcg agataggttg acgggtcttg
     2761 ataatcaggt ccccaaccgc catgatataa atcataatct ttctgagcag ctgtttgagc
     2821 aaagtagcct gaactgtcaa actcatctga tgttaattgc tgaatgtcaa tcactacatt
     2881 atcagaacct aaaacagatt caattgattg tttgatagaa ctaactcctt gtatgcctac
     2941 tttatctgtt acttccacag tcttatccaa gtggattggg aattgaacac cctttgcttc
     3001 gagttctttc ttagcttccg caaacttagc cttggctttc tcaggattgt agtaagggtc
     3061 ttgaccatcc gcaaagttga taccttgcca ttccttacca tagttgacca tcttagaggc
     3121 tacaacttca ccaaagtctt ttcccttgat actgacaaag tttggaggaa ccactaggtt
     3181 acgcaaaatc tttgttgcac cttctttccc ttcagactga gccccataag atgttctgtc
     3241 aaaagcaaaa ttgatagcct gacggaagtt tttattgaga actgcttcct gagtcgattt
     3301 cttttcaatg tcacttgttt tagaagtata attgtaagac ttcctatcta ggttaaaatt
     3361 aaagaaatat gaagttgaat tttgcatact atagatgata ttgtttttgt atttttcttt
     3421 aatcccttca tagctggagc tgttaggaaa aagacgagcc gtagtataag caccagctgt
     3481 aaaattacgt tccagtgatt cttggtcgct accatcatag taggtcaatt tcacatcgtc
     3541 tacaaagaca ttcttagcat cccagtaatt agggtttttc ttatattcaa tagcagattt
     3601 tgagacaagt gctttcatca agaaaggtcc attgtacaaa atactagatg gatccgcctt
     3661 cccaaaatca tccccttttg atttcaggaa atctgcatta acaggaaaaa gtatcgttgc
     3721 aagtgttttt gaattccagt aaagttctgg tttaaccaaa gtatattgaa ccgtttggtc
     3781 atcaagtgcc ttgacaccga cagttgaaaa gtcgcttgtt ttaccagtga tatagtcatc
     3841 caaaccagca acagagtcct gcactagata caaggcttct gattttttat cagctgcata
     3901 ttgcaaacct gtcacaaaat cctgggcagt tacaggcgca tattcttctc cctcagaagt
     3961 aaaccacttg gcatccttac gaagtttgta ggtataggtc aaaccgtcct gagaaacagt
     4021 ccaatcctct gctaatgatg gaataatatt cccatattgg tcattttcta ataacccgtc
     4081 taccaaattt gcaacaatat cggatgttgc tgcgcggttt tctgctagat agttcaagct
     4141 agatggatca cttgaataaa catagttgta ggtttttgac gccgtgctag aatttccaca
     4201 cgcgctcaat aaaactcctg tacccaggac aagacctgcc aaggttagat atttgctctt
     4261 agactttttc atttccggaa cctcctgctt taaatatttg ttttattata caactcttgg
     4321 tttgtttagt caattgtttt ttccaaaaaa tcaaaaaaac ctcattttct atttttgaaa
     4381 gataatttta gctattttta cgctaaaaaa ggaataagtt tcaagttctc ctaaaagatt
     4441 gttcaactta ttccttttat ttataaatgc taccttacga aagcttaaat tttgctcgca
     4501 ggcgatctgc ttgggctttt cctattacct tatctaataa ataggtacgc agactcataa
     4561 ccatataaag tccacccccc atggcaccga caagagctac ataaaagaag ctccacaaac
     4621 gtccacttgg ttggaagaaa aatcctaaca gccactggat ggttcctatt aacagaaaca
     4681 tgactagggt cagcaaactg attaaaatgg ttcgcttcaa aatcaccttg cgcttgacac
     4741 cagttacttt acaaatatcc cgatacatca agacgttagg aatgatgaga gcaatggttg
     4801 ttgaaatcaa aggaccataa ctgtggaaga gggcgatggt aggtagttgc aagactagct
     4861 tggcaataga accatagata aaatagagaa cggccttgcg gttgcggaac atggcctgaa
     4921 gcattggaga caagaccatg tacaagccta aaataataga ctgcaaaact gcaaagacaa
     4981 ataagcccag agccaaacta tctggcttac catagaagac cgtataaaga ggttctccta
     5041 ccataaccac tccaaccgtt gctggtagca agaacataaa gagtagggtg agactgtcct
     5101 gaacgagacg agaagctgct ttcaagtccc ccttgacata gttttccgtc aaaagtggca
     5161 aaccaacact cccaatcgaa acccctacag aaatcaaaat catcgtgatt ttattaggat
     5221 tggctgagaa ataagaaaac atgacaacca agtcctcatt gctgtagttg gtaaaccagc
     5281 tcatactatt gataaaggtc agctgatcca aaatctggaa gagctggatg gcagaccctg
     5341 tcaggataaa aggaatggct tccttaatgg tatcaaccaa gagacgctta ctgttaatct
     5401 tatctcctgt ttcaaagatt cttttgagtg aaccttcttg ggcaaggaaa taaatcaaga
     5461 ctgcaaaact ggctaccata ccgacaaagg cagcaaaggt tgattgggta acggctgcta
     5521 gataatctcc tgaaccgagc ttcataatga taaaggttgc taggagcatc cagataacac
     5581 gaatgacctg ctcagcaatt tggctcatgg catagggttt gaggttattc atcccttgga
     5641 aaaatcctcg gataacactc atagacggga aaatcaagac tccccaagcc aagctttgca
     5701 tgattgggat caagtctttg cccacgccag acaagtctgc tagccaagga gcaaagacat
     5761 acaagactaa agcaaaaacc aggcctagtc ctgtcataaa gcctaagaag ctccgaatca
     5821 gggcaaagct atgctcttct tctcgcatgg tattatactt ggcaacttgc ttggccaccg
     5881 caactggaat ccccgctgtt gaaaccaaca agaaccaagc atagatattg taacccatgg
     5941 taaagagacc atttgcctta gctgcataag cccccatcca gatgtaccaa gggataatgt
     6001 aaacagcccc gagtaggcga ctgataaagt tactagccgt tagccaagca gtcccccgta
     6061 acatctgggc ctgctggtga ttgttttcgt gcgacataga ttcctcattc aattttgata
     6121 actaggaaaa actccttatc ttccattata aacttttcct tgttacttgt aaatttacga
     6181 ctttcggttt acaatagaaa gtatgattaa gattgaaacc gtattagata ttttaaagaa
     6241 agatggcctt tttcgcgaaa ttattgacca aggtcattac cactacaact acagcaaagt
     6301 tatttttgat agcatcagct acgacagccg aaaagtaaca gaagacactc tttttttcgc
     6361 aaaaggcgct gcctttaaaa aagaatacct tctttctgct ataacacaag gattagcttg
     6421 gtatgtagct gaaaaggact acgaagtcgg tatccctgtc atcattgtga acgatataaa
     6481 gaaagccatg agtttgattg ccatggagtt ctatggtaat ccacaggaaa aactcaaact
     6541 ccttgccttt actggtacta agggtaagac aacagcagcc tatttcgcct ataacatctt
     6601 atctcaaggg catagacctg ctatgttgtc gaccatgaac acaactcttg atggcgagac
     6661 tttctttaag tcagcgttga caacccctga gagtattgac ctctttgaca tgatgaatca
     6721 ggctgtgcaa aatgaccgta cccacctcat catggaagtc tccagtcaag cctatctggt
     6781 caaacgtgtc tatggtctaa cctttgatgt gggagttttc ctaaatatca gcccagacca
     6841 tatcggcccg attgaacacc ctagctttga agactatttc taccacaagc gtctcttgat
     6901 ggaaaagagc cgagcagtca tcattaacag tgacatggac cacttctcag tcttgaaaga
     6961 acaggttgaa gatcaagacc atgatttcta tggtagccaa tttgataacc aaatcgagaa
     7021 ttccaaagcc tttagctttt cagctacggg taaactcgct ggagattatg atattcaact
     7081 cattggcaac ttcaaccaag aaaatgcagt tgctgctgga cttgcttgtc tccgtctcgg
     7141 agcaagtctt gaggacatca aaaaaggcat cgctgcaacc cgcgttcctg gtcgtatgga
     7201 agtcctcact cagaaaaatg gagccaaggt cttcatcgac tatgcccaca atggggatag
     7261 tctgaaaaaa ctcatcaatg tggttgaaac tcatcaaacc ggaaagattg ctctggttct
     7321 gggatcaaca ggaaacaagg gagaaagtcg tcgtaaggac tttggcctcc tcctcaatca
     7381 acaccctgag attcaagtct ttctgactgc tgatgaccct aactatgaag acccaatggc
     7441 cattgcagat gaaattagta gctacatcaa tcatcctgtt gaaaagattg cggatcgcca
     7501 agaagccatc aaggcggcaa tggctatcac aaatcacgaa ttagatgcag ttattattgc
     7561 gggtaaggga gccgattgtt accaaatcat ccagggcaag aaagaatcct acccaggaga
     7621 tacagccgtc gcagaaaatt atttataaga atagaaaaaa tcaagggaaa atgaaaccct
     7681 tgattttttc ctattcttta gtaaaaacgt ttttcaaacc ttcaacggca ccttctacag
     7741 caccttttgc gtcttctaca acgtcttttg ccttagcaac tgttttttca acagctcctt
     7801 caagttctgt cttgctgtct ccagtaactt taccaaaacc ttctttgaca gcgcctgttg
     7861 cttgttccaa tttgttttca agtgacatat gattgtctcc tttagtttat tccgatatgt
     7921 gttaccggtt acatatagtt tataccgaat actaaggaaa gtcaaacaat gtactcagaa
     7981 acaagaaatc acgctaagta gaaagttaac ctctccttat caaaatcgat agccaactca
     8041 tactttccat cttcattttg cacaatatag cctaagacaa cctaactgtc cacaaagata
     8101 tcacggcatt tctgcataag ctggtctttt ttgagaaatt tcaacaaaaa agccgtcata
     8161 tacttgaggg catactcagg attaacatct cctaaaatgt catagagctc ctgctgtttt
     8221 tctgtcaaag gatactgctg tttgaccttg taaaaataat tggacagggt cattttaatg
     8281 cgcgcaaagt ccgtcttttc aactaaaata gctgcattga tttgattgcg caattccgtc
     8341 tcaaaactct gctccaacaa ggcttgatag accggactag cttctctgac aaaaatctct
     8401 tgatccagtt caagactatc aagtgattca agcgtaggaa aattgaggta atagcgctta
     8461 ttttcacgta gaatcaagcc tgcctttata tactcttcca tgagtttatc aactgcaaca
     8521 tctggaaatt gagccttaat ttcccgtaga atcacatcct catactgatc cagatagcgg
     8581 atcaattctc caaaaatggc tgtctcgtca aacgagatgg attaaaaatc tgaatcatga
     8641 agattccttt ctttacattg taacagaggc gatgctgcca actgactagg attggtacca
     8701 gcttggttca gaggaacagg cagacctagt tctatgtaat actccctcca aaaatcacta
     8761 atctcctgct ccccatctcc aaatttcata ggaatagttt caaaaatagg taggatttct
     8821 gctatatact gggagcagta aaaaccagct gcatctggat agaaagaagc attgtagggt
     8881 gctccaagat gtttgcaagc tctttccttc accgactgga tatccatttc tgggtaaaca
     8941 tagaggtcgt ataaatgatt ggactcaaag aagtctgccg gttcttgaca gacaacacca
     9001 gcctgtccac tagcatgata aatcatccca tccaaataaa tggcaacatg gctatagtta
     9061 cctgtggaag tctggatggc ctgtcccatg tctgacccat ctctcacaaa aatcaaatcg
     9121 ccattttcta acatacttca ctcctagaaa aaaggagccg aaactccttg cgatataagg
     9181 caaactagac tttccgattt tgagctgaca cccaaaatct tacgcattaa agctttcagt
     9241 caattgaggt accacttgtt tcttacgtga aacggcacca gcaaggaagg catgattgtt
     9301 ttcaagtttg aagttgaaag ccgcttcgac cttgtccata ttggcaccaa gagccaagat
     9361 ttctgagttt gagttgacga tatctgtaat catcaagaca aagtcagagt agccgtttga
     9421 ttcgttggca gcttgcattg cagcttcaat ttctgcttgg cgttccaaaa cttcagcgat
     9481 gtcaactgtg ttcacttggg caacacggac attatttccg ttgagttcaa aagtcttagc
     9541 atcgatatca atcaattctt cagcagattt gctagccaag ttggtaccag ctttcaacat
     9601 tgccaaacca tattcttcca agttcacacc agccaattca gccaattcag gagcaatgat
     9661 tttatctgtt gggtgtgttg ttggtgattt caaaagaagg gtatctgaaa tcaaacctga
     9721 aagcatcaaa cctgcaatct ctttaggcac agctacacca tgttctttga acatacggta
     9781 aacgattgaa gacgctgatc caactggctc caaacgcatg taaagtgggc ttgcagtttc
     9841 aaagttagcc acacggtggt ggtctacaac accgtaaact tctacttcag cgatatctga
     9901 tacagattgt tggaattcat tgtggtcagt caagataact tgctctgccc cctctgcttt
     9961 ggcagaagtg ataacacgtg gtgcttccac accaaaatag ttcaagacaa aggctgtttc
    10021 ttcatttgga gttccaaggg caacagcttc cgtatccaaa ccgtaagctt cttttgcaag
    10081 gtaggcaaaa gctacagatg atccgatggc atctgagtct ggattttggt gaccaaatac
    10141 tagaatcttg gacatgataa tacctcattt tgtttatttt ctttattgta gcattttttt
    10201 cgctttttga caaaggtaag aggagtcaaa ggactcccca cgattcttca aaataactga
    10261 gtaaatttct atcttgattt tcagataaaa attcttcctt ctgtggcctc ttcttacgct
    10321 tgagaagagc ttcagccgac atggcttctt ccttactggc aaaaccttga gcatagataa
    10381 gtttgactgg caagcgtgct cgtgtatatt tggctccctt cccactattg tggatagcga
    10441 ggcgtcttct catatcagtc gtatagccta tatagtagga tccatcacga cactccagaa
    10501 cgtacatata agccttatga tccataataa atctcttcga tttcgggcgt ataagagcca
    10561 tcatcattgt ggacaatcaa aggaggtaag accttaaagc cacttgttga gccatccttg
    10621 atcgcctcaa tcaaaagcat attggcttcc ttttctcttt ttggataaac aaactgcagg
    10681 cgcttagggg ctagattatg tcgttttaac gtatccaaaa tatccagaag tcgatcagga
    10741 cgatgaacca tggccaaacg cccattagac ttgagaatac tctgggcact acgacagatt
    10801 tcttccaaat tagtcgtgat ttcgtgtcga gccaagagat aatgttcact ctcgttcaga
    10861 ttagaataag gattcacctt gaaatagggt ggattacaca aaatcatatc caccttactc
    10921 ccctgaatgt gagcaggcat atttttcaaa tcatcgcaga tgacctgcat ttgctcctct
    10981 aatccattca aacggacaga gcgttcagcc atatccgcca aacgctcctg aatctcaaca
    11041 gacaatatct gtgcttgagt acgagtgcta gcaaaaagcc ccactgctcc attcccagca
    11101 cagaaatcca caatcaaccc cttcttagga aaacgtggaa atcgtgataa gagaacacta
    11161 tccaccgaat agctaaaaac ctctctattt tgaatgattt tgatatctgt cgaaaagagc
    11221 tggttaatgc gctctcctga ttttaataat tgttcttctt ccatggtcct attatagcaa
    11281 attcatatta acattacaaa aaatataaaa ctctaaacta cttcttcttt tttaaatggt
    11341 gcagggcttc tccagtccag attggtagca ttcgtcgaaa gggagcaaag ccgtagttaa
    11401 agcggtcgct tgaaaagcgt ctccgtctag gaaactggta cttttcttcc tccaaagtgc
    11461 ggatagaaag actggctttc cctgtaaatt catctaaatc cactacctga acttgaacct
    11521 cttcatcgac tttcaaggtt tcatgaatat tttcaataaa tcctgtccga atctctgaaa
    11581 tgtgaatcag ccccgtatca cccgtctcta actcaacaaa ggcaccgtag ggctgaatcc
    11641 ctgtaatacg cccctttagc ttatcaccga ttttcatctt agtcctcgat ttcaatagtt
    11701 tcaattacaa catcttcaac tggcttgtcc atagctcctg tctcaacagc agcaatggca
    11761 tccaagacag cgtaagatgc ttcatcagct aactgaccaa aaaccgtgtg acggcggtct
    11821 aggtgaggtg tcccaccttg attggcatag atttctgcaa tcggttctgg ccaaccacca
    11881 cgagtaattt ctttcttaga ataaggtagg tgttggtttt gcacgataaa gaactggctg
    11941 ccgttggtat ttggaccagc atttgccatg gaaagagcac cacggatatt gtaaagctct
    12001 tctgagaatt catcctcaaa agattcgccg tagattgact cgccacccat accagttcca
    12061 gttgggtctc caccttggat cataaagtcc ttgataatac ggtggaaaat gacaccatca
    12121 tagtagccat cttttgaaag agatacaaag ttagccactg ttttaggagc atgttcaggg
    12181 aaaagcttga tacgtaagtc tccgtgattg gtcttaatag tcgcaagagg accttctact
    12241 gtttcaatgt ctacttgtgg aaaatgcaat tctttttcta ccataccaaa tacttctaag
    12301 gcagcaaaaa tgccatcttc ttctaatgtt tttgtaatat aatctgcttt ttctttgatt
    12361 ttatcatgag aaattcccat ggcaacgctg attccagcat aatcaaagag ttccaagtcg
    12421 ttgagaccat ctccaaaaac catgaccttc tctggtttca agccaaggtg ttccacaacc
    12481 ttttccaccc ccgtcgcttt ggagcctgaa atcggcacaa tatcagacga atgttgatgc
    12541 caacgaacca tgcgaagttt gtctgagaga ctgtcaggca agtgcaagtc atctccctta
    12601 tcttcaaaag tccacatctg atagatatct tctttttcat ggaaatcggg atctacatct
    12661 aagtcgggat aaattggatt gatagcttca ctcatcatat cggtgcgagt cgacaacttg
    12721 gcatcatgac tcccaaccaa gccatactca attccttctt gcttagccca agagatatac
    12781 tcctcaacat ctgacttttc aatctgatgc tgataaatga cctgaccttt tttatcttcg
    12841 atataagccc cattcaaagt tacaaaaaag tcaggcttga gatcacgaat ctctggaaca
    12901 acaccaaaaa tgccacgtcc agaggcgatt cctgttaaaa ttcctttttc acgcaactgt
    12961 ttaaaaacag tgggaattgt agttggaata aaccctgtct ttgaattccg caatgtatca
    13021 tcaatatcaa aaaagacaat cttgatcttc tttgccttgt atcttaattt cgcgtccatc
    13081 tcactacctc tttcaatcta actctttcca ttatatcata aagtaggcaa atcccctatt
    13141 ttcaaaaagt ttatcatttt tattttaatt tcttggatga gaaaagagac atatttatga
    13201 aaaagctcca tcgtgctttt aatgtgttct cttgttttca aactcgtaaa aagggagcca
    13261 ctgatcctaa ctcgctctct catttcaaag cttgtgaaaa aagacccgtt ggggtcttaa
    13321 ttcgctttct tgttttcaag ctcatgaaaa agagacccaa ctgggtcttt tctttaatct
    13381 tcgtttacga aaggcatcaa agccattacg cgagcgcgtt tgatagctgt tgttacttta
    13441 cgttggtttt tagctgaagt tcctgttaca cgacgaggaa ggattttccc acgttctgaa
    13501 acgaaacggc taagaagctc agtatctttg taatcaacat attcaatttt gtttgctgcg
    13561 atgtaatcaa cttttttacg gcgtttgaat ccgccacgac gttgttgagc catgtttttt
    13621 ctcctttata agtttagttg tccattagaa tggtaaatca tcatctgaaa tatccaatgg
    13681 gtttgttgct ccaaatggat tttcattacg tgaaaagtct ggtactgaat ttgtaggtgc
    13741 tgaatagttt gcagttggtg cagagtaagc tccacctgtg tgaccctcac gcacactacg
    13801 gctttccaac atttggaaat tctcagccac gacctctgtc acgtagacac gttgtccttg
    13861 ctggttatcg taactacgag tctggatacg acctgtcacc ccgataagtg agcctttttt
    13921 agcccagtta gcaagatttt cagcctgttg gcgccacata acgacattga taaaatcagc
    13981 ctcacgttca ccattttgac tcttaaatgt acggtttact gcaagagtaa aagtcgcaac
    14041 tgctacattt gatggggtat aacgcaactc agcgtcacgt gtcatacgcc ctacaagtac
    14101 aacattgtta atcatagttt accttcttac gcgtcaattt tgacgatcat gtgacgaaga
    14161 atgtcagcgt tgatttttga aagacggtca aactctttaa gagctgcatc gtcatttgct
    14221 tcaacgttaa cgatgtggta aagtccttca cggaaatctt ggatttcgta tgcaagacga
    14281 cgtttttccc aagtttttga ttcaacaaca gttgcaccgt tgtcagtcaa aatagagtca
    14341 aaacgtgcta ccaaagcgtt tttagcttct tcttcaatgt ttggacgaat gatataaaga
    14401 atttcgtatt tagccattga tatgttcctc cttttggtct aatgacccca agactttgca
    14461 aggggtaagt gaggttcgct cacaataaac tattatacta gaaaaaattt ttttacgcaa
    14521 gtaaaaacac tagaattcga aaaaacgcca catgggcgtt ttcctgttct tatggtttga
    14581 tacggtgcaa catacgtggg aatggaatag cttcacggat atgttttgtt cctgctgcga
    14641 aggttaccat acgttcgata ccgataccaa atcctccgtg tggaactgta ccgtatttac
    14701 gaaggtcaag gtagaattca tattctgtac gatccatgcc aagttcatcc atcttagcga
    14761 caagggcatc gtaatcttcc tcacgcatag acccaccgat aatttctcca tagccttctg
    14821 gagcaagcaa gtctgcacaa agcacgcgct ctggatttcc aggaactggt ttcatgtaga
    14881 aggccttgat ggctgctgga tagttcatga caaatgttgg cacaccaaag tggtttgaaa
    14941 tccaagtttc gtgtggtgac ccaaagtcat caccatgctc aagatgctcg tagtcagcat
    15001 cttcatcatt ttcatgctct tgcaagaggt caatggcttg atcgtaagtg atacgtttga
    15061 atggctctgc aatgtagcgt ttcaagagtt ctgtatcacg ttccaaggtt tccaaggctt
    15121 gaggcgcgcg gtcaagaaca ccttgtagaa gagctttcac ataagcttct tgcaagtcaa
    15181 gcgactcatc atgtgtcaag tatgagtact cagcatccat catccagaac tcagtcaagt
    15241 gacggcgtgt ttttgatttt tcagcacgga aaactggacc aaagtcaaag acacgaccaa
    15301 gagccatagc ccctgcttct aggtaaagct gacctgattg gctcaagtag gctggcgttc
    15361 cgaagtagtc agtttcaaag agttctgtag aatcttctgc cgcatttcct gaaagaattg
    15421 ggctgtcaaa cttcataaaa ccgttcttgt caaagaactc ataagttgca tagataatag
    15481 cgttacggat ttgcaacaca gctacttgct tacgagagcg tagccacaag tgacggttat
    15541 ccatcaaaaa gtctgttccg tgttcttttg gtgtgattgg gtagtcttga gattcaccga
    15601 tcacttcgat gtctgtgatg tccaactcat agccaaattt agaacgttcg tcctctttga
    15661 caatacctgt cacataaaca gacgtttctt ggctcaagcg tttgataaca tcaaacttct
    15721 caagtcccac ttcttcacca aatttttcga caaagtttgg tttaaaagcc acaccttgaa
    15781 agaaggctgt tccatcacgc aattgtaaga aagcgatttt tccttttcct gatttgttgg
    15841 caacccaagc gccaatcgtc acttcctgac caacatagtc ttttacgtca ataatcgtta
    15901 cacgttttgt cattattttt ccttttcttt tttattcttt atggcaaacc acctctatat
    15961 tgttcccatc caggtcaatc ataaaagcag catagtaaat cggatgctca cttcgataac
    16021 caggagcccc attgtctcgc ccacctgcct ctaagccagc ctcataacaa gcctgaactt
    16081 cttccttatt ttctgctaaa aaagcaaaat gaacaggatc ttgtgttccc tgagtcagcc
    16141 aaaaatcacc accaggatga gggctgttcg gggatagaaa actaattaga gaactagtct
    16201 taaaagccaa tttatagtcc aaaggagcga gaaaactcct ataaaatcct tatgaaattt
    16261 gtaaatcctt taccttaatc tcaaaatgat caatcattct cactacccat aaatgctttc
    16321 aagcgttcga ctgcttcttt aagcgtgtct aggtctgtcg catagctgag gcggacattt
    16381 tctggtgctc caaatccagc tcctgttacc aaggccactt cggcttcttc taagataaca
    16441 gttgtaaagt ctgtcacatc cgtgtagcct ttcatctcca tggccttttt gacatttggg
    16501 aagagataga aggccccttg cggtttgacc acttcaaatc ctggtacctc tgcaaggagg
    16561 ggatagatgg tattaagacg ttcctcaaag gcctgacgca tgctttctac agtatcttgc
    16621 tcacctgata gagcctcaac tgctgcatat tgggctactg ctgacggatt cgaagttgtt
    16681 tgacctgcaa tcttggacat ggcagcgata atgtctgctt ctccaacggc ataaccaatc
    16741 cgccaaccag tcatggcata agttttagac acaccattga tgaccactgt ttgcttgcga
    16801 atcgcttccg ataggctaga aatcggtgtg aactcatgac cattataaac caagcggcca
    16861 tagatatcgt ctgctaggat gagaatatca ttttctacag cccagtttcc aattgccaag
    16921 agttcctcac gggtgtaaat catacctgtg ggattagatg gcgaattcag caccaaaacc
    16981 ttggtcttgt cagtgcgagc tgcttctaac tgctctacgg tcaccttaaa gtgattgtct
    17041 tccttagcag aaacaaagac gggaacgcct tctgccatct tgacctgatc tccatagcta
    17101 acccagtatg gggttgggat gatgacttca tcacctggat tgaccacagc cataaagaag
    17161 gtatagagag aatatttggc tcccgcagcg actgtcactt gatttgacgc tacagaatag
    17221 ccgtaaaagc gctcaaagta gctattgacc gccgccttaa gctctggcag acctgaggtt
    17281 actgtataaa aagaagcacg cccatctcga atcgatgcaa tggcggcatc ttggatattt
    17341 ttgggagtag tgaaatctgg ctcacccaag gttagagaca aaatatctct accctcagcc
    17401 ttcagtgctt tggcacgggc tccagcagcc aaagtcacac tttcttccat ttctaaaaca
    17461 cggttggata gtttcatagg ccctccttgt tgaccaatgc tcctgtttca aaatctacta
    17521 gataaaaatc agatcctgac ttaacttccc agattggctt atcttgataa cggccaaagg
    17581 ttatcttgtc aatctcgcca gctccctttt ccttagaaac cgtttctgct ttttcttgtg
    17641 aaacaccctg atttagctga taaacgtaaa tcttatggtc atctttacca atcaggacag
    17701 caagcgcttc ttgctgtttg ttacgaccaa gaacgctgta ataagattcc aagccattgt
    17761 ataaatcaac ctgatcagcc tgctctaatc ctgcatactg ctgagctaat ttttctcctt
    17821 cacttttagc tgtttgatag ggtttcatgc taagagaaac catatacaga aaggaaccac
    17881 tgataaccac aaacaaaatc gtcatcccta gaccatactg ccacagtaga ttattttttg
    17941 ctttgttttg tctttttttc actcgtctat tttaccatct attaagcttt attacaagtg
    18001 aatataagaa tactcttcga aaatctcttc aaaccacgtc agctttatct gcaacctcaa
    18061 agctgtgctt tgagcaacca attctatttc tcccttcaaa caaaaccgat tttgaaagtg
    18121 aaacagttct tactttttca gtcacaaatg attagagttt ccgaaatttt acaattttca
    18181 aataaactct gagcaaattt cttgcaagtt cttttgtttt gttgtaatat attttataac
    18241 aacgagagag ttctcgaaat tttaagaaaa aggagacaca tcatgtctaa aaaagtatta
    18301 tttatcgtcg gatcactacg tcaaggttct ttcaaccacc aaatggcgct cgaagctgag
    18361 aaagcacttg ctggtaaagc ggaagttagc taccttgatt attcagccct tcctctcttc
    18421 agccaagatt tggaagttcc aacacatcca gctgtagctg ctgctcgtga agcagttctc
    18481 gttgcggatg ctatctggat tttctctcca gtctacaact tctctatccc tggtacagtg
    18541 aaaaacttgc ttgactggct atctcgtgcc cttgacttgt ctgatacacg tggcgtttct
    18601 gcccttcaag acaagtttgt cacagtatca tctgtagcca atgcagggca cgatcaactt
    18661 ttcgctatct acaaagacct cttgccattt atccgtacac aaggcgttgg tgatttcact
    18721 gctgcacgtg ttaatgactc tgcctgggca gacggaaaat tggttcttga agaaacagtc
    18781 ctaaactcac ttgaaaaaca agctcaagac ttggtcgaag ctatcaagta actaacactc
    18841 aataaaaatc aaaaagcaaa ctaggaagct agccgcaagc tactcaaagc actgctttga
    18901 ggttgtagat agaactgacg aagtcagtaa catatatacg gtaaggcgac actgacgtgg
    18961 cttgaagaga ttttcaaaga gtataacata ataaaagcga acaagactac ttttctgaca
    19021 ctcagaagac tagcttgttc gcttttttct catttataaa ctaaaatagc tgatgataca
    19081 tattgccaaa tacaaaatat ctgatttcta tacttccttt acaaaaacca atccctgtgg
    19141 ttgtgacaaa tcttctcggt aaacaaatcc agcaaagttc aagcgacgtg cttcccccac
    19201 agtttgtact tgattttcta ttaaagctgt tagaaaggcc ccgcgcgctt tcttggagat
    19261 agttgagtga atcttcagct gaccgcctct atcctccatg aatttgaagg tcaccatctt
    19321 tgctctgatt tccttagaaa atacagtctc aaactctgat gacaagagag agaaaatcac
    19381 ttcttccttc ttcagagttt catcataggc tgccttccaa tggctcttca aagtcttacc
    19441 agcgactttt aatttcatca aaaaatccaa acggtgagga gccatgggtg acaagactgg
    19501 aacaacaccg tacaaagccg aggtaatgaa aacatgattt tcaagataat cttgttccgc
    19561 ctcggtcagc ttatctctct taatgttgcg gtacataagc ccatcaaaaa gtttcaaggc
    19621 tggatagtgt tgagcagttt gccttttcaa agcttggata ttttgaaatt cttccgccgc
    19681 tttctcagct gataccttgt agaaactctc caattgactg gcagaataga gagccaaggc
    19741 atcaagcacg gcctgacttt ctggttttaa aggaattgcc tcgatacttg ggaagtctgt
    19801 gttcatttct tttgctgttg ggattaaaat tttcatattg atagtgtagc atatttttta
    19861 gccacaacca agaaattcat ttgaaaaacc tcggtttgac cgaggttttt ctatttattt
    19921 gggccatcct aaccagacag ccacgagaac aagggctgtg ccagctaaac ttgttaagat
    19981 agataagaat ttatcttttt tctccttgaa ctgagaaaaa cctatcttca aagcgagcag
    20041 tccgagtagc attgaagcaa ccatcaaata aaaacccatt tctttgtcct ccattaggct
    20101 taatttacct tattataaca tatactttct gaaaaaaacc tatttttacc gtagcttaaa
    20161 ggtcttgaga taattgtctt ttcctacttg accttcgaag gttttaccat tttcaaggta
    20221 aggaaggtca tcggatactg aagccttgac cttgtatatc ttgccatcaa ctttaaagaa
    20281 gtagacagtg tctcccttga taacagctga tttgaggtct gctactactc cattgatgct
    20341 ttctgtcgtt gcattgtcaa tttcaaggtc gtttttattg gcatacttgc tgagcatctc
    20401 ttccactgta gtagcaacga taacattttg gtactcgact gcgtctacca gggcgtactc
    20461 tttgaccaag ccagcattgt ccttcaagcc catgatgtag agaggcttgt cattgaggtt
    20521 gatgaggatt gggaaggttg ctttgtagga tttctcctga acagcacctt ctgctgattc
    20581 acgggctgat tcttctgtcg cagaagccaa gctatactta gtgatttctc ctgttcgcat
    20641 attttcaagg atgaaaccaa gattactctc atccgcatta gccgacgtca cacctgtgta
    20701 gagatagatg tcattaccga tagacaagta attatagcca ttggtagtct gggtcacgtt
    20761 tttcttggaa atcatggcat tcaagaaacc gtccttgtac ttgccgttgt agttgatttg
    20821 ctcaatggtt tcctctgctg gatagatcct gtccacccat tctggaacat ctgataagct
    20881 gtattccttg gtttctccat ttgtagcatc caagataatg actgaagcag gacgaggaac
    20941 agcaagtcca aattgctttt ggtaaaccgt tgctacatag aaaggattgc cctcatcgtc
    21001 cacctcaaaa gatggagttt taaagatttt ggtcgggtac ttcaagcgca ggtgacgttt
    21061 gacatcacgg ttaaaatact ccgagtctga atacttgatt ggtgtcttca agtccaccaa
    21121 atccgcattt ccagttacca tgtccacctt aatatactca ccgattccct tggcttgatt
    21181 gttaaaccat ttgatagggt ctgcatattc tagtggtgtg acccgataag gtttcccatc
    21241 aattgtcaat tgggtatagg tatctgccgc tacgtattgc gacaccttat cggttaggga
    21301 acccaagtag cggtctccaa ttttttcagc agtacttcta tctaggatag gaaccttact
    21361 ggtgtcactc ttaggaaatt cagtaaagtc tttttccgta accgtgacta cattggcata
    21421 atttttagcc tgaaacatgc tggaagttac caaggaaacc aaagctgcca agagaagaat
    21481 tcctccaatc gaagctaaca gaattttccc taaccgattg attttgaaac cctcaagatt
    21541 taaggcagct tccgccttac cgtggcgcac atgaaccgtt ttgacaaggt ttatcccctt
    21601 gccaaagcca aataatattg ccacaactaa caaatgccca cagaggaaga agagaaattc
    21661 ccagctggtc aggttaaggg gcggtaaaaa gatataccag gtcgttgcga taaaaataag
    21721 ttcaaagact attcgtttca tttgctttcc tcctaaatga ttcgtccttc caagtgatcc
    21781 agctcatgct ggcaaatctg agctgggaag cctgtcaagg taatggtctg ttcctgccac
    21841 ttgctgtcac gataggcaag ccttatggtt tcataacgct tagttgatct cacacctacc
    21901 aaggacaaac agccttcttc tgcctcatag gatccttcaa aggacagaag cactgggtta
    21961 aacatgacca caggaaccaa gccaagatta aagataatca cgcgcttctg caccccaatc
    22021 atattggcag ctagaccaac acaggtatca cgatttgcta agagtgtatc ctgcaaatct
    22081 ctggcaagat aaaggtcctc ctgacttgcc ggctgagaca cttgagataa aaataagata
    22141 tccttcacaa ttttcttttc cacttcctca cactcctctt gctttttact atattatagc
    22201 aattatagca caaaagccga taactgcaaa ccctttccct caactgtagc aaaaagccat
    22261 ccgaagatga cttttttgct atttaatttc tgtataagtt acttccaagc cacgcttaac
    22321 agctggacga ttggcaattt tttctgccca ttttactaga ttttgataac ttgaggcatc
    22381 caagaatttt gcagaacctt ggtaaagatt tccttgaact aactgtccat accaagacca
    22441 gatagcaata tctgcaatcg tatagtcatt gcctgcaata taaggtttct gagccaattc
    22501 cttatccaat aaatccaact ggcgtttcac ttccatcgta aaacggttaa taggatattc
    22561 caatttttca ggagcataat tgaagaaatg tccaaatccc ccacctagaa aaggtgctgc
    22621 acctgcttgc cagaatagcc aattcaaaac ttctaccttt tccacaggat tacttggtaa
    22681 aaaggctcca aatttctcag caaggtaaag aagaatatga gcagactcaa agactcttac
    22741 gttttcagta cctgactggt ccaataaggc tggaatcttg gaatttggat tgagcttcac
    22801 aaagtctgat ccgaattgat ccccatccat gatagcaatc ttatacaagt cgtaagccgc
    22861 ttccttaaaa ccagcttcta gtaattcttc caataagata gtaaccttca caccatttgg
    22921 tgttcccagt gaataaagct gaaaagcttg ttctcctttt ggcaagtttt gttcgaaacg
    22981 ggcacctgct gttggtctgt ttagccccgt aaaagctcct tgattactag cttcatcctg
    23041 ccatacggtc ggtaattgat atgctgacat ccgaaacctc ccttaaatcg cattcttgtc
    23101 aaaaccgagt ttgcgttgaa taaacttaac gatttcgacg atgataatca ttgagaagct
    23161 tccagccata acaattcccc attgtgacaa gtctagtttg gttacgtgga agattccttc
    23221 aagcggttct acaacgattg ttgccatgag aaggataaag gataccaaga tggaccagtt
    23281 aaaggtctta gacttgaatg ggccaactgt caagatggat tggtagacag acttgacatt
    23341 gtaggcatgg aagagctgaa tcaaaccaag ggttgcaaag gccatcgtta gggcatctgc
    23401 atgaatagca tgattgtcac ccacatgaac tgggtaagca atcgcaaggc cataaacact
    23461 cataacaaga gctgcttgga gtacaccttg ataaatgata gaactcaaaa caccacctga
    23521 gaagaagctt gccttgcgtc cacgtggttt atgattcatg acaccaggtt ccgcaggttc
    23581 aacaccaaga gcgatagctg ggaaggtatc cgttaccaag ttgatccaca aaagatgaac
    23641 cggctgtaag acatcccaac caaacaaggt tgataggaag atggttaata cttcagcagt
    23701 attagcagaa agtaggtact gaatagtctt ttgaatgttt gagaagacct tacgtccttc
    23761 ttccactgcg acgataatag tcgcaaagtt atcatctgca agaatcatat cagaagcccc
    23821 cttagaaacc tctgtaccag tgattcccat accgataccg atatcggctg ttttcagagc
    23881 tggcgcgtca ttgacaccgt cacctgtcat ggcaacgact ttaccttgtt tttgccaagc
    23941 cttgacgata cgaaccttgt gttctggaga cacacgggca taaacagagt attgaccaac
    24001 gactttttca aattcttcat ctgacagttc attgagttca gcaccagtta aaacgtgacc
    24061 ttctgtatcg tttgcgtcaa tgattcccaa acgtttggca atggcttccg ctgtgtcttg
    24121 gtggtcacct gtaatcataa ttggacggat tcccgcttcc ttagccacac gaacagcctc
    24181 agcggcttca ggacgttcag ggtcaatcat cccaatcaaa ccagtaaaaa ttaaatcatt
    24241 ttcaagctct tcagaagtga gattttctgg aatactatcg ataatcttat aagcacctgc
    24301 aaggacacgc aaggcttgat gagccatttc agaattgttt gtacgaatga gatttgtaac
    24361 cttctcatca atcggagcaa tatccccagc cttatcacga agaagacaac gttttaagag
    24421 ttggtctggc gcacccttga ctgctacaag gaaacgacca tctggcaatg ggtgaactgt
    24481 tgacatgagc ttacggtcag agtcaaatgg caattcagct acacgaggat atttctctaa
    24541 gaaacctttg acatcatagc ccttgtccaa ggcatattgg ataaaggctg tttcggttgg
    24601 gtcaccaatc aagttacctt ccacatcgat tttcgtatca ttggccaaga caactgaacg
    24661 aagtagtggc atttcaagac ctagttcaat atcatcagct gagtcatgta gaaccgcatc
    24721 gtagaagact ttttcgactg tcatcttgtt catagtcagc gtaccagtct tatcagaagc
    24781 gatgatttca gttgaaccaa gtgtttcaac tgctggcaac ttacgaacga tggaatgtcg
    24841 tttggccaaa acttgagtac caagagaaag aacgatggta acgatagcag gaagtccttc
    24901 tggaatggct gcaacggcaa gggcaacaga agtcaacaac tcaccaagtg gatttttccc
    24961 ttgaatgaag acacccacta caaaagtaac aagggcaatg accaagatag cataggtcaa
    25021 gaccttagaa aggttgttca aattttgttt gagtggtgta tcagtctcat ccgcatcttg
    25081 aagcatacca gcaatatgac caacttcagt gtacatacct gtattgacaa caacacccat
    25141 cccacgacca taggttacgt ttgagttttg gaaggccatg ttgacacggt caccaatacc
    25201 agcatctgtc gcaagctcga ctgacaagtc tttttcgact ggtacagatt cacctgtcaa
    25261 ggctgcttct tcaattttaa gagagttggc ttctatcaaa cgtaggtccg ctggtaccac
    25321 gtcacctgct tcaagggcaa cgatatcgcc tggtaccaat tctttagagt caatctctgc
    25381 catgtgtcca tcacgaagaa cgcgggcaac tggactagac atggatttga gggcttcaat
    25441 agcttcttca gcttttcctt cttggtaaac accaaaggca gcgttgatga taaccacagc
    25501 taggatgata atggcatctg cgatatcttc cccaccagaa gtcacgactg acaagattgc
    25561 tgccgcaact aggatgataa tcatcaaatc cttaaattgc tcgatgaatt tgaccaagat
    25621 tgatcgtttc tcgccttctt cgagttcatt gtgcccaaat tcggcaaggc gcttttccgc
    25681 ctcacttgat gacaaacctt gctcggtcgc atccacagcc tgcaagacct cttcagggct
    25741 ctgagtataa aacgcttggc gtttttgttc ttttgacatg tgtctcctcc ttgacattgt
    25801 gtgcaaaaca gactctcttt ctgtcatagc ttttcacgac aaacaaaaag aaacctgtta
    25861 atcataacaa gtctcgctgt ttaagatagg gccggaaagc atacttttca gcataaaatt
    25921 cggaatgacg acactatcac aggtttctgc cagctactcc cttgagtagt accattatac
    25981 caaattttgg ggagttttca aagagtaaaa actgccttat ttgaattttt ccttgaaaac
    26041 cagtataatg gtagaatgct atgtgactag aaaggaagtt gaatgaagca atctatctca
    26101 aatctcaagt tagctgagcg tggagccatt atcagtattt cgacctattt gatcttgtct
    26161 gcagccaaat tagcagctgg tcatctcctt cattcatcca gtttggtggc cgatggtttt
    26221 aataacgtat cggacatcat tggaaatgtg gccctcttaa tcgggattcg gatggcgcgc
    26281 cagcctgcag accgtgacca ccgttttggt cattggaaga ttgaagattt ggcaagcttg
    26341 atcacttcta tcatcatgtt ctatgtcggt ttcgatgttc taagagatac cattcaaaag
    26401 attctcagtc gggaagaaac ggtcattgat cctcttggtg caactctagg aatcatttct
    26461 gcagcgatta tgtttgtggt ctatctctac aatactcgcc tcagtaagaa atccaactcc
    26521 aatgcgctga aggcagctgc taaggacaat ctttctgacg ctgttacctc acttggaacc
    26581 gccattgcca tcctagctag tagtttcaat tatccgattg tggataaact ggttgctatc
    26641 atcatcactt tctttatctt gaagactgcc tatgatatct tcatcgagtc ttcctttagt
    26701 ctttcagatg gctttgacga ccgcctgctc gaggactacc aaaaggctat catggaaatt
    26761 cccaaaatca gcaaggtcaa atcgcaaaga ggtcgcacct acggtagcaa catctacctg
    26821 gatattacac tagagatgaa tcctgacttg tctgtttttg aaagccatga aatcgcggat
    26881 caggtcgagt ctatgctgga ggagcgtttt ggcgtctttg ataccgatgt ccatatcgaa
    26941 ccagcaccta tccctgagga tgaaatttta gacaatgtct ataaaaaatt gcttatgcgt
    27001 gaacaattga ttgaccaagg aaaccaacta gaagaactct tgactgatga ttttgtctat
    27061 attcgccaag atggagagca gatggataaa gaggcttata agaccaaaaa agagttaaat
    27121 tctgctatca aggacattca aattacttcc atcagtcaaa aaaccaaact catctgctat
    27181 gagttagatg gtatcatcca taccagtatc tggcgtcgcc acgaaacctg gcaaaatatc
    27241 tttcatcaag aaaccaaaaa agaatagaga aatcctttca tgagacggga tttttctatt
    27301 cttttatact caataaaaat caaagtgcaa attaggaagc cggtcacagg ctgtacttga
    27361 gtcggcaatg tgaagccgac atagtttgca ctttgatttt cgaatagtct taactatcaa
    27421 attcactgag atactcatag cgttcgtatt tttcaaggag tgcttcattt ttctcatcca
    27481 attctttttg gagagtagcc agcttaccaa agtcagagcc gttagcctgc atttcctctt
    27541 caatagcagc gatacgtttt tccaaggttt caatatcacc ttcaatactt gcccactcct
    27601 gcttttcttg gtaggtcatg cgtttcttgt cttctcgaac cttgaccact ttttcctttt
    27661 cggccttttg cacttgattg gccatatctg tttcaaaagc tttttcatca agatagtcgg
    27721 tgtaatgacc aaagaaagga cgaatcttgc catcctcaaa agcgagaatc ttggtcgcta
    27781 ccttatccaa gaaatagcgg tcgtgactga ctgttaaaac gggacctgca aaaccttgca
    27841 agaaattctc taagactgtc aaagttgcaa tatctaggtc attggttggc tcgtctaaaa
    27901 gaagaacatt tggtttttcc aaaagcagtt tgaggagata aagacgtttt ttctcacccc
    27961 ctgacaattt ctcaatcaaa gtcccatgcg tcgaacgtgg gaagaggaat tgctccagca
    28021 actcagcgat ggaagtcgta gaaccaccac tggtcttgac ctcctctgcc acttcctgca
    28081 ggtaattgat cacacgcttg ctttcatcca aaccctcaat ttgttgagag aaataggcga
    28141 tgcgaacagt ttccccaatc acaacttgtc ctgctgtcgg ctcaagactt cctgcaatca
    28201 ggttaagtag ggttgatttt ccaacaccat tgtccccaac aattccaata cggtctttag
    28261 cctgaactaa gagattaaaa ttttgcaaaa tgggcttatt ttcataggca aaggaaacat
    28321 cctgaaactc gatgactttc ttcccaatcc gactggtttc aaagttcata gtcaagtctg
    28381 tctcagcact actgcctgaa acttcctttt tcagatcatg gaaacgattg atacgagctt
    28441 gttgcttggt cgcacgcgcc tgcggttgtc tgcgcatcca ggccaattct tgtttgtaga
    28501 gttgttcttt tttgtgaaga agagccgcgt cgcgctcatc ctgttccgcc tttaggcgaa
    28561 catagtcctg gtaatttccc tggtactcgg tcaagcctgc acgatccaac tcgaaaatcc
    28621 gtgttgacaa agcgtctaag aaataacgat cgtgagtgat aaaaaggacg gtcttcttag
    28681 aatttttcaa aaagagggtc agccactcaa taatcgcaat atccagatgg ttggtcggct
    28741 catccaaaag caagaggtcg tggttgccaa gtaagacttg tgccaactgt acccgtcttc
    28801 tcagaccacc tgacaattcc ccaacaggag tagataagtc ttgaatgccc aatttgctaa
    28861 gaacggtctt gacctgactt tcgatttccc aagcttggag agagtccatc tctgccatga
    28921 cacgttccaa acgcgcctgc ttgtcctcac tatagtcgag cataatcaat tcatactcac
    28981 gaatgagctg gatttccttg agttcactag atagaaccgt atccaaaact gtctttctat
    29041 catcaaaatc aggatcctga gtcaagtaac caatctggta atcattttta gctgaaaaag
    29101 gactgacatc cccatcaaat ccagaaacac cagaaaggac gtccaaaagg gtggtcttgc
    29161 cagtcccatt gacaccgatt aaaccaattc tgtctaagtc atggataata aaggaaatat
    29221 ccctaaaaac ggtcttgtca ccaacggatt tacttagttt ttcaacgata aaatcactca
    29281 ttttttctcc ctcaggtaag catggatggc ttcacgatta ttctccaatt ctccatcgac
    29341 aatggcaaac tcaatctctg ttaaaatctc tcccaagtct gggcctggct gatagccata
    29401 ttccttgatc aaaataccgc cattaatctg aatctctttc ttgtcatgga tagtcaagct
    29461 ttggtatttt tctgtgatgg cttgtgggtt gacttctttt ccttgagctt gacgaagatt
    29521 ttcagcctgt aaaagcaaat ctatgtcaaa gcgataacaa tctcgcttgc tcaattctcc
    29581 attttcacgc agagccaaaa taatcagcaa atcctgaact tgcttggcaa actggcgtga
    29641 ggtcttccaa gatttcaaaa atgactgcgc attttcaatc tccaaagccc atagtaaagc
    29701 cgcccaggct tgttcagagg attcaaaagt aaaatcagtc tccaaatcaa acagtctgtt
    29761 gagcttgtcc tggctagatg ccatatcagg gagatagtca taagcttgac tctcaatcat
    29821 ggaagccaag ccccttctcc aaaatggagc cagcaagagt ttatcaaact cgacgaaggt
    29881 acgctctaca gaaattttct ccaaaagcgg cgtcaaggtc ttcatagctt taaatgtttc
    29941 tggctcaagt gcaaaaccaa gactagcctg aaaacggaaa ccacgcataa tccgtaaagc
    30001 atcttcgttg aaacgctcac tagccactcc aactgctcgc aagacttgct tttccaaatc
    30061 ttctaaacca tggaacaagt caacgatttc tcctgtctca tccaaggcaa aggcgttgac
    30121 tgtgaaatca cggcgtttga ggtcttcttc tagcgatcgt acaaaggaaa ccgcactggg
    30181 tctgcgatag tccacataga catcctctgt ccgaaaggtt gttacctcat actcctcatc
    30241 cccatctaag accaagacgg ttccatgctc gattccgata tcggctgttc gcggaaaaat
    30301 ctgcttggtc tcttctggat aagaagacgt cgcaatatcc acatcgtgga tagggctatg
    30361 gagaagggca tctcgaacag agcccccaac aaaataagcc tcaaagcctg cttctttaat
    30421 tttttctaat actggtaaag ccttctgaaa ttcagaaggc atttgcgtta atctcataat
    30481 aagtgttcta atccatagac aagctcatga cgcttgacaa cttctttaat tcccaaattg
    30541 actcctgtca tgaaggagat gcgatcatag gagtcatgac ggagggtcaa cccttctccc
    30601 tgattgccaa agatgacttc ctgatgagct accaagcctg gcaaacgaac tgagtggatg
    30661 cgcataccat caaagtcagc accacgagca ccagcaatca gctcttcctc atctgctgca
    30721 ccttgctgaa ttgactctcg aacctctgcc atcaactcag ctgttttaat ggctgttcca
    30781 ctcggagcat cctttttctt gtcatgatgg agctcaataa tctccacatt tgggaaatat
    30841 ttggcagcct gcgtcgcaaa ttgcatgagt aagacagcac ccaaggcaaa gttaggggca
    30901 atcaggccac ccaagtcttg ggcacgagaa aattctttta gctctgcaat ttcttcactc
    30961 gtgaaaccag tcgttccaac tactggagca aagccatttt caagagcaaa acgtgtattt
    31021 tcgtaggcaa cagctggagt agtaaaatct acccagacat ccgcttcaaa accagctaaa
    31081 tcagccttat ccttgaaaac aggaataccc tgccattctg actcagactc aaaaggatcc
    31141 aaaactgcca ccaagtccaa gtctggatca gtcaatacca tctgacaagc agcctggccc
    31201 atctttccct taaaaccggc aataattact cgaatactca tctctactcc tgtctaagat
    31261 acaaagtccg taagaacaca aagtgaaaat aggaattcca atcaagaagt gtctacttct
    31321 tggaagaact atctttttca cacagggttc caggcgtgtt caattatcaa gatacaaagg
    31381 accttagctg cctctgaaaa atagggaatg gcactgactt tccacgaaag gcaagacagg
    31441 catctttttt caagaggcag gtagtccgtg ttcaatttct aagatacaag gcatcttaac
    31501 tagcctagaa gcgccaacta aatcactgga atataaccca gagcaatact tcctgctcct
    31561 aggtgcgttc caatgacact accaaatgta gcaagtgaaa catccgaacc caagccaaaa
    31621 tcaagcaagt gctgacgcaa ttcttcagcc ttttcaggag cattcccatg aatgacaatg
    31681 acccggtatt gacctgaagc cgttgtttcc ttgataattt caattaagcg cttggtggcc
    31741 ttcttttcag tacgaacttt ttcgtaaact tcaatcacac cttgatcgtt aaaataaagg
    31801 attggcttaa tgctaagcaa attgcccaaa atggcagccc catttgaaag gcgtccacct
    31861 tttaccaaat gatccaagtc atctaccatg ataaaggctg acgtacggct gatttgaatg
    31921 gctagcttat cctgaatgct ggcaaaatca tcgccctgat cacgccaatt aaagacgctt
    31981 tcaaccatga tgcctagggg agcacttgta atcaaagtgt ctgggaaagc aatggttaag
    32041 ccctcatagt catcgaccat atactggata ttttggtaaa aacctgaaat tccagaagat
    32101 aggaaaagcc ccaaggcatg tgtatagcct tgttctttga gcgaagttaa gatctcatct
    32161 aacttggcaa tacttggttg actggtctta ggcaattcag aagcctgagc cattttttgg
    32221 taaaattcct cagcagacag attgatgcct tcgacatatt cctcaccatc aatattgaca
    32281 ggaatatcca agacaaacaa gtcttctctt tgcaagatct ctgcactgag ataagcagag
    32341 gaatctgtga aaacagctaa tttcatatta gaactccaaa ttaattcctg gtaagtctaa
    32401 tgcaatttca gtcacttcgt aagtcaaacg attgagcatg ttcaaacatg gacgagccaa
    32461 ggtttccacc tcttcttggt tcaattcact tggttcattg acaatacggc catcgatatg
    32521 gtttacttgt gagattgttc cactaatgac aaacttatca aatacaatca taaagctcaa
    32581 gatgacaatc aaggaagtca cttgattttc ttggtcatgt tggagcaatt ggaaattcac
    32641 atccaccttg gtttcaggag ctccattttc attttcccat tcaaaattac gcgcatcaaa
    32701 atgatactga ctaacaaatt cttgttcacg tttaagattc atgtctttct ccatcggcta
    32761 caatattata agctattgta ccataatttt ttattttcat ctagttttct aggatttagt
    32821 caatcccaat ttcagcacga actacatctg tgatggtatc aacatagtag tttacttctt
    32881 ctgttgtagg cgcttctgcc ataacacgca agaggggttc tgttccactt ggacgaacaa
    32941 ggatacggcc gttccccgcc atttcttctt ccatcttctc gatgatggcc ttgatagctg
    33001 gcacttccat ggccttttcc ttcatgacgt tttccactcg gatattaact aatttttgtg
    33061 gataaatcgt tacttctgcc gccaactctg ataagctctt accagtttcc ttcatgattt
    33121 tagtcaattg aactgctgat aattgaccat cacctgtggt attgtaatcc atcaagataa
    33181 cgtgaccaga ctgttcacca ccaaggttgt agcctgattt tctcatttct tcaacaacgt
    33241 agcggtcacc aactgcagta actgccttgt taataccttc gcgattcaag gccttgtgga
    33301 aaccaaggtt agacataaca gttgtcacaa ttgtattttg agccaattgt cctttttcag
    33361 aaaggtattt tccgatgatg tacataatct tgtcaccatc aacgatgtca ccattctcat
    33421 caacagcaat caagcggtca ctgtctccat caaaggccaa accaatagct gacccacttt
    33481 ctttgaccac ttcttgaagg gcttctggat gtgttgaacc aacattaagg ttgatgttaa
    33541 gaccgtctgg tgtttccccg ataaccgtca attgggcacc aaggtctgca aagatttgac
    33601 gggcactggt agaagctgct ccattagctg tatccaaggc aaccttcatt ccatcaagag
    33661 gagttccagt tgaaacaagg tatccttcat acttacgcaa gccttctgga taatctacca
    33721 aaattcctaa gccttctgca cttggacgag gaagagtgtc ttcctcagca tctagcaagg
    33781 cttcaatttc tgcttctttt tcatcatcta gtttgaagcc atcaccgcca aagaacttga
    33841 ttccgttatc aagggctggg ttgtggctag cagaaatcat gacaccggca cttgctcctt
    33901 cagtttcaac caagtaagct actgctggtg ttgcaaggac accaagtttg tatacgtgaa
    33961 tccctactga aaggagacct gccaccaagg ccgattccaa catttcccct gaaatacgtg
    34021 tgtcacgtcc tacaaagact ttcggcgctt ccgtttcatg ttgactaaga acatagcctc
    34081 caaaacgtcc tagtttaaag gctaattctg gtgttagttc taggttagct tctccacgga
    34141 ctccatcagt cccaaaatat ttacccattg ttataaaatc cttttctatt tttaattcgt
    34201 ttttgaacta gttgctttcg ttgacgaaga tgtctccgat gaactgcttg tacttgaatt
    34261 tgatgtgctt gaacttggtg ctactggttt tgtagtcacc ttcattattg tatcaaacgg
    34321 agtgataact gccggtaaga caacaccatt gcggtcgatt gcctgcaaag gtactgaacc
    34381 actgtaatta cctgttatac gttcgctagt tggcaaaaca gcgataatct tatcaattct
    34441 atccaatgtc tcttggtcac tcgtaataga cacttcttta tctgacacca tgacattttc
    34501 aatttgtacc cgactatcaa tttgactagg gtcaatctct ggtacaatct ttaccttatc
    34561 cttctgagcc ttcttaccaa tcttgactgt aattttttgc ggagtcgcca cagcggtcag
    34621 cccattgggt aaatcttcaa tgctcaaagg aacttcaatc gttccaacac cggcatctgt
    34681 taggtcagca gtaaccttga atttacgtgt actttcttgc atttcactag ctagcgatag
    34741 gcgatttgca ccagtcaaga ccactgatac ttctgaagca aaaccgctaa taaaatactt
    34801 atcactatta tagcgtatgt caatagggac atttgttact gtattagtat aggtttccgt
    34861 ttttacctgc ctagcactgg tactgttttg aaaattcgtc gccgtagcat agacaaataa
    34921 gacacaagca aaaaagagtg aggatatgat atataaacta ttttttttca tgtttccatc
    34981 ctcctagcaa tcgttcttta aaactaagac ccacttcctc ttttggaagt aagatttcac
    35041 gtaattctgt ttcaaattca tcaagtgtta ggttgtgctt aaaccttcca ttataggtta
    35101 tcgaaattcc tcccgtttcc tctgatacga caaaagtcaa ggcatctgag acttctgata
    35161 aaccgatagc cgcccggtgt ctggtcccaa attccttgga aatccctgtg ttttttgtca
    35221 agggcagata ggcagacgtc acagcgatac gttcttcttt gataatcacc gcaccatcat
    35281 gtaggggagt gttgggaata aaaatgttaa tgagaagttc tgcagaaatc ttagcatcca
    35341 agggaattcc tgtcgaaata tactcctgca aggtacgtac acgctgaata gcaaccaagg
    35401 ccccgatttt acgaggactc atgtattcaa cagacttaac aaaggcacga atcatctgtt
    35461 cctcagcact aataggggca ttggaaaaga aatctgtcgc tcttcccaaa cgttccaaac
    35521 cagtccgaat ctctggagag aagataacaa ccgccgcaat aaccccataa gtaataattt
    35581 gattgattaa ccaagaaatc gtagtcaaac caatcatatt tgcaaggatt tgagctaaaa
    35641 taaacaccaa aactccacgt accaaaatca taatcttggt tcctgcaata gcttttgtaa
    35701 aatggtataa aatataagca acaatcaaaa tatcaatcag attgatagct atcgtccatg
    35761 gacttgcaaa caaactggtc caatattgca gattggataa ttgttgaaaa ttcatccctg
    35821 atatcctccc tatcaaaaca ctttcgtcct attataccat tttctggcat ttttttccct
    35881 atcctagtcc attttacatt gaacaaaaat atgataaaat aaactgacta aaaaaaacaa
    35941 aggagaaact atgtctcaac tctatgatat taccattgtg ggtggtggtc ctgtcgggct
    36001 ttttgcagcc ttttatgccc acctacgcca agccaaggtt caaatcatcg actctcttcc
    36061 ccagctaggt ggacaacctg ctattctcta ccctgaaaag gaaatcctag acgtaccagg
    36121 cttcccaaac ctgactggag aagagttgac taaccgcttg attgaacagc taaatggatt
    36181 tgatacccct attcatctca atgaaacggt tcttgagatt gacaaacaag aagaatttgc
    36241 catcacaact tctaaaggaa gtcacctgac taaaacagtt atcatcgcta tgggtggcgg
    36301 tgccttcaaa ccacgtccgc tggaacttga aggggttgag ggctatgaaa atatccacta
    36361 ccacgtttct aacattcagc aatacgctgg taagaaagtg acgattcttg gtgggggaga
    36421 ctcggctgtg gattgggctt tggcttttga aaaaatcgca ccaactaccc ttgttcaccg
    36481 cagagataat ttccgtgcct tggaacacag tgttcaagcc ttgcaagaat catctgtaac
    36541 catcaagaca ccattcgccc ctagccaact ccttggaaat ggaaaaacac ttgataaact
    36601 tgaaatcaca aaagtcaaat ctgatgaaac tgaaaccatt gacctagacc acctctttgt
    36661 caactatggt ttcaaatctt ctgtcggtaa ccttaaaaac tgggggctcg acctcaaccg
    36721 tcacaagatt atcgtcaaca gcaaacagga atccagccaa gcaggtatct atgctatcgg
    36781 tgactgctgc tactatgacg gaaaaattga tctgattgcg acaggcctcg gagaagctcc
    36841 aactgctgtc aacaacgcta tcaactacat tgaccctgaa caaaaagtac aaccaaaaca
    36901 ctctactagt ttataaaaaa gaaccacgag tcacatagga ttcgtggttt tataattcat
    36961 ccgctatctt attgattttt ctgagtctgt gattgacacc acttttggtc agaggggtgc
    37021 tgagactatc tgctaactgc tggatagagt agtctgggtg ctgaatcctc agttgcgcca
    37081 cttcctgcaa atctactggc aaattttcta agcccatgat atctttgatt ttactgatat
    37141 tgttaatggt cttcatgctg gcagaaactg tccgagcgat attagctgtc tcggcattat
    37201 tagcccgatt gaggtcgtta cgggtttctc gcaaaatctt aacccgctca aaatcatcac
    37261 gtgcctgcat ggctcctatt actatcaaga agtccataat gtcttctgct cgctggagat
    37321 aggtcacagc ccccttcttg cgctcaagca ccttggcatc cagtaaaaac tgttggagaa
    37381 gggaggcaat tccttgcgcg tggtccagat aaacagaact gatttccaac tggtacttgc
    37441 ctgactcagg gtcacgaatg ctcccatttg ccaagaaagc gccacagaga taggcacgac
    37501 ctgcttcctc atccgataaa atcgcctcat caatacctgt ttccaggcca aagaaagagt
    37561 ctgccaagtg caaatcactt aacaaatcct gcaccttttc atctgtaaaa acggtataga
    37621 cgcgattctt gcgaagattg ctccgttggt ggtgacgaat ttcagatttg atttcataga
    37681 gatggagaaa ggactcatag aggtgacggg ccagtttggc attttctgtc acaactgaca
    37741 aagtcaagcc cgaagtcgag agaccgatgc taccagacat tttgataatg gcagataatt
    37801 catgccagct cagatggtgt tggcccagga tttcttcttt tactgctact gtgaaactca
    37861 ttttttcacc tgtataatgc gcatcaactc gtccacaatc aaatctccat cgtggaaggc
    37921 accgccattt tccagacgaa ggaagttaga tgaaatcacg cgcgaaactt gcttacaaag
    37981 acctacaaaa tcgtgttcca cttgcactaa gtattcatca aaacggttgg aattcatgta
    38041 ttcctgaggc actttttcaa tattcaccaa gacagtgtcg ataaaagggc gaccaaggtg
    38101 acgatgcaag acttccacgt ggtcgctatc tgtaaagtgt tccgtctccc cacgttgggt
    38161 catgatattg cagacatagg caatttctgc cttggtttcc aaaagagccc gcccaatttc
    38221 cttaatcacg atattgggca aaatagaggt aaagagggaa cctggcccta ggacaatcat
    38281 gtcactttca aggatggtct gcactactcg acggctggcc agaggcgtat catcgtttag
    38341 ggcattggtc acatagacat tgtcaattat gcctcgatgg tctacaatat gactctctcc
    38401 agccacttct gtcccatcct gaaagactgc atgaagggtc aaaggatggt cactggaagg
    38461 ataaattttc cctgttgtat ggaaaaattt gctcaataac tgcatggcat tataggttga
    38521 accctgcatt tctgacaagc cagcaatgat gagatttccc aatggatggc cagcaaaggc
    38581 tccggcatcc tcagagaacc gatactgaaa gaccttctca taaaacttag gcatatccga
    38641 catggccaca aggacattac gaagatcacc tggcggtgtc aactgttgca tattttttcg
    38701 gagttcacct gaagaaccac catcatctgc caccgtcacg atagctgcga tttccacatc
    38761 tttttcccgc agacttttta gaatgacggg acttccagtc cctccaccaa tcaccgttat
    38821 ctttggtttt ctcatgaacg gtttaccgtt tcctttctgc ggtctttgtc gcgatgccct
    38881 tcattaacag accaattctt ggataagtcc tgcgccaagc gtttagcaaa tgccacacta
    38941 cggtgttgtc cacccgtaca tcccatggca atggtcaaaa cggacttacc ttccttttgg
    39001 taacttggca gaatcggctc aatcaaggcc aataaatgtt gataaaagtc ttctgactca
    39061 ggatggttca tgacataatc ataaacaggt tcatccacac ccgtttggtt tctcagttct
    39121 ggtaaataat agggatttgg caagaaacgg acatcaaaga ccaagtccgc atcaatcggg
    39181 attccatact taaatccgaa agacatgact tcgatacgga aagactgggc ttgttcttgg
    39241 tctgaaaact gctctgcaag ggttttgcgc agctcacgtg gagtgagttc agtcgtatcc
    39301 accacatttt ggctcatatt tttcaaaggt gccaagagtt cacgttccaa cttgattcca
    39361 tctaaaatac gaccgtctgc tgctagtggg tgactccgtc tggtttcctt gtaacgagcg
    39421 accaattcct tatcagccgc atccaaaaag aggattttga aatccaaacc atcttgattt
    39481 tccaactcat ccaaaacagc ttgaatctct gaaaagaaag aacggctacg catatccact
    39541 accaaggcca acttaggatt gtcttcctta atttcaacca gctgcaaaaa cttaggcaag
    39601 agagctggcg gcatattatc aatggtgaaa taacctagat cctcgaagga ctgaatggct
    39661 acagttttcc ctgcgccact catccctgtc acaatcacca agtgaagttg tttctttgtc
    39721 atctttttct ccttatatca aaagaagttt ggcaacacca aacttcaact agcttatcca
    39781 atctctgcga tgacttcaat ttcgactttt acatcacgag gaagacgagc tacctccaca
    39841 gctgaacgag ctgggaattc ctctttgaag gccgtttggt aaacctcatt aaaaggaaca
    39901 aagtcgttca tatcgctcaa gaagcaagtt gttttgacaa catggtcaaa gtctgttcct
    39961 gcttctgcca aaatagcacc gatgtttttc aagacttgct ctgtctgttc ttggatattc
    40021 tctcctacaa tttccccagt ttcaggggat aggggaactt gaccgctagc aaacaaaagg
    40081 ttgccaacga tttttccttg aacatagggt ccgatagcct ttggggcctt atctgtatga
    40141 attgtttttg ccattttctt ttcctcacaa tttttctaag attgcatccc aagcctcatc
    40201 catccctgcc ttactgacag atgaaaagag gatgaaatcg tcactcgggt caaagtttaa
    40261 tttctttttg attgctgatt catgcttgtt ccatttacca cgaggaatct tgtccgcctt
    40321 ggtcgccaca atgatgactg gaatctcata atacttgaga aattcgtaca tctgcacatc
    40381 atctgctgac gggtcatgac gaaggtcaac tagactgaca accgcacgga gattttcccg
    40441 agtcgttaag tactcctcaa tcatgcaccc ccacttttca cgttcctttt tagaaacacg
    40501 agcatagcca taaccaggca catccacaaa gcgcatcttg tcatcaatgt taaaaaagtt
    40561 caggagctgg gttttaccag gttttcctga tgtacgggcg agattcttac ggttcaacat
    40621 agtgttgata aagctggatt taccaacatt tgaacgccct gctagggcaa tctctggcag
    40681 ttcatcctgc ggatagtggg acttattagc tgcactgagc aagatttcag cattgtgtgt
    40741 attaagttcc atagtcacct ctaggctgtt tctaggatcg gtttatccgt tccatctaca
    40801 gtttctttag tgatgcgaac caatttcaca ttttcctgac tcggcacctc aaacatgaca
    40861 tctagcatgg tttcttcgat gatggagcga agtccacgcg cccctgtctt ccgttcgatt
    40921 gctttattag caatctcttg aagggcttcg tcgtcaaatt ccaactcaac atcatcataa
    40981 gaaagcaagg tttggtattg tttcaccaag gcatttcttg gctctttcaa gatgcgaacc
    41041 aagtcatcaa cggtcaattg ctcaagagcc gcaaaaacag gcaagcgtcc aatcaactca
    41101 gggataatac caaatttttg aatgtcttca gcgatgattt cttgcatgta tgagctgttt
    41161 tcgtcaatcg ccttattatt ttgaccaaat ccgatgactt tttcacccag acgttgtttg
    41221 acaatttctt caataccatc aaaagcacca cccacgatga agaggatatt ttttgtatcc
    41281 acttgaatca tctcttgttg tggatgtttg cgtccacctt gaggcggtac gctagcaaca
    41341 gttccctcaa taatcttgag aagggcttgt tgcacccctt caccagaaac atcacgtgtg
    41401 atagacacat tctcactctt cttggcaatc ttgtcaattt catccacata gataatgcca
    41461 cgctctgcac gttcgatgtt aaagtcagca acctgcaaga gtttgaggag gatattttcc
    41521 acatcctcac ccacataacc agcctccgtc agagctgtcg catccgcaat agcaaaaggt
    41581 acattcaagc tcttagccaa ggtctgggca aggaaagttt tccctgaacc agttgggcca
    41641 atcatcaaaa tgtttgactt ctgcaaatcc acatcttctg actcttcgcg tgtatcgtgg
    41701 aaattgatgc gtttgtagtg gttataaacc gccactgcca aggcacgctt ggcacgatct
    41761 tgaccaatta catagtggtt caagatatgg aggagttcaa ttggttttgg cacctcagac
    41821 aagtctgcca agacttcctc aaccaattct tctcgaatga tttcctgagc taactccacg
    41881 cattcattac aaataaaagc attgttgcca gcaattattt tttgtacttc ttcttggttt
    41941 ttgccacaaa atgagcaata aaccatcata tcattttttc tatttgtaga catgatttcc
    42001 ttccattcta tactgtcatt ctatctaaaa taaggtcatg taaaaagcat gaatactatt
    42061 gaccagattg gtaaaggcat ttaaccaaag gaggatagaa agcccgtaac gctttttacg
    42121 aaaagcttgt gctcctgcca gaaagcagat gaaacacaga aaagccgtga atagaccaaa
    42181 taaactccgt tccattagac ttcctttctc ttgcggtatt ggatggtaaa atcataagga
    42241 ttcttctcat ctttggcgta aaatttgctt gaaactgtct caaaaagaga caagtcaagt
    42301 tcttcaggga aataggtatc tccttccacc cgagcatgaa tgtgagtgac aatcacttca
    42361 tcaaggtaag gttcaaaagc ctgaaaaatt tgcttcccac cgataatgta gagattcttt
    42421 tcttgagcct gataccagtc aagaacagac tggacgtcct gaaaagtagc aaccccatct
    42481 atcttttctt ccggattacg cgtcaaaatc aaggtttccc gttttggaag caagcgacgc
    42541 cccatcccat caaaggtcac acgccccatc aagatagcat gattcagagt tgtttcttta
    42601 aagtgctgca attctgctgg caaatgccaa ggcagacgat tttccttacc aatcacaccc
    42661 tcttcatcct gggcccaaat agctacgatt ttcttagtca tgcttccatc cttttcactg
    42721 atagtactat tttatcaaaa aactcaaaaa aagactggtt tggaatagct tacaaaatag
    42781 aaaaaatctg taagaaattt cctacagatt tatctatgtt tccttatttc ttacaaacca
    42841 ggtgcttgtc caagttcggc tgcaagcatc caaattgttt tatctgtttc agttttagcg
    42901 cctgcaaaga taccgtttgt cacatcgtca ccttcttcat cagtgacatc caaacctttt
    42961 tggaaaagtt ctgacaagta acggtagata acaagaacac gttccaagct ttcttcaaca
    43021 ttacggtatt caccagcttc ttcttcgatt tcactatttt gaaggaactc tgtcaatgta
    43081 gagaatgggc ttccaccgag tgtaatcaag cgttcactga tttcatccaa ttgaccgtca
    43141 agagcttcca tgtactcatc catttttgga tgccatacaa ggaaaccacg accatgcata
    43201 taccagtgca cttggtgcaa agcaacgtga gctacataca aatcagcaac agcttggttc
    43261 aagacttcct ttgtttttgc caatgctact ggcgctgctt ttgtcaatga tgttacgtct
    43321 tttactgctt cttttttcaa ttctaccatt ttctttacct cattcattat tttttgtaac
    43381 cacttcttaa tgattactac cttagtatac tactaaggaa aaccaatagc aagacaaaga
    43441 acttatatga gaaaagttgc aatagactga taatttaaga attctttaga agacaaaaag
    43501 agggttggat gcttatagtc aatggaaatc catcagcaaa ctaggaaact aaacccagct
    43561 tgaccaaagc acaagttttg aggtcgcagg tagaactgac caagctgatg tggtttgaag
    43621 aaattcccca atactataat agattgaaat aagatgaaac caagtcgatt aggaaaggta
    43681 aatgaatttc taaaatg
//
LOCUS       DBDESG010000011        42829 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.011, whole genome shotgun sequence.
ACCESSION   DBDESG010000011 DBDESG010000000
VERSION     DBDESG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.011"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(20..1978)
                     /gene="ftsH"
                     /locus_tag="ACKB38_000579"
     CDS             complement(20..1978)
                     /gene="ftsH"
                     /locus_tag="ACKB38_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGL7979397.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(1994..2536)
                     /gene="hpt"
                     /locus_tag="ACKB38_000580"
     CDS             complement(1994..2536)
                     /gene="hpt"
                     /locus_tag="ACKB38_000580"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGL7979398.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2540..3817)
                     /gene="tilS"
                     /locus_tag="ACKB38_000581"
     CDS             complement(2540..3817)
                     /gene="tilS"
                     /locus_tag="ACKB38_000581"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGL7979399.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3814..5082)
                     /locus_tag="ACKB38_000582"
     CDS             complement(3814..5082)
                     /locus_tag="ACKB38_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGL7979400.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5075..5197)
                     /locus_tag="ACKB38_000583"
     CDS             complement(5075..5197)
                     /locus_tag="ACKB38_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGL7979401.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            complement(5202..5570)
                     /locus_tag="ACKB38_000584"
     CDS             complement(5202..5570)
                     /locus_tag="ACKB38_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGL7979402.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5563..5829)
                     /locus_tag="ACKB38_000585"
     CDS             complement(5563..5829)
                     /locus_tag="ACKB38_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGL7979403.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5887..9396)
                     /gene="mfd"
                     /locus_tag="ACKB38_000586"
     CDS             complement(5887..9396)
                     /gene="mfd"
                     /locus_tag="ACKB38_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGL7979404.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(9397..9966)
                     /gene="pth"
                     /locus_tag="ACKB38_000587"
     CDS             complement(9397..9966)
                     /gene="pth"
                     /locus_tag="ACKB38_000587"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGL7979405.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10037..11152)
                     /gene="ychF"
                     /locus_tag="ACKB38_000588"
     CDS             complement(10037..11152)
                     /gene="ychF"
                     /locus_tag="ACKB38_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGL7979406.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11236..11430)
                     /locus_tag="ACKB38_000589"
     CDS             complement(11236..11430)
                     /locus_tag="ACKB38_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGL7979407.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11495..12631)
                     /gene="dnaN"
                     /locus_tag="ACKB38_000590"
     CDS             complement(11495..12631)
                     /gene="dnaN"
                     /locus_tag="ACKB38_000590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGL7979408.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12790..14151)
                     /gene="dnaA"
                     /locus_tag="ACKB38_000591"
     CDS             complement(12790..14151)
                     /gene="dnaA"
                     /locus_tag="ACKB38_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGL7979409.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14364..15122)
                     /locus_tag="ACKB38_000592"
     CDS             complement(14364..15122)
                     /locus_tag="ACKB38_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGL7979410.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15180..16361)
                     /locus_tag="ACKB38_000593"
     CDS             complement(15180..16361)
                     /locus_tag="ACKB38_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HGL7979411.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16544..17023
                     /gene="rlmH"
                     /locus_tag="ACKB38_000594"
     CDS             16544..17023
                     /gene="rlmH"
                     /locus_tag="ACKB38_000594"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGL7979412.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17067..17140
                     /locus_tag="ACKB38_000595"
     tRNA            17067..17140
                     /locus_tag="ACKB38_000595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17101..17103,aa:Arg,seq:tct)
     gene            17305..17430
                     /gene="comC"
                     /locus_tag="ACKB38_000596"
     CDS             17305..17430
                     /gene="comC"
                     /locus_tag="ACKB38_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGL7979413.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17451..18776
                     /gene="comD"
                     /locus_tag="ACKB38_000597"
     CDS             17451..18776
                     /gene="comD"
                     /locus_tag="ACKB38_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGL7979414.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVIFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18773..19525
                     /gene="comE"
                     /locus_tag="ACKB38_000598"
     CDS             18773..19525
                     /gene="comE"
                     /locus_tag="ACKB38_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGL7979415.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19577..19648
                     /locus_tag="ACKB38_000599"
     tRNA            19577..19648
                     /locus_tag="ACKB38_000599"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19610..19612,aa:Glu,seq:ttc)
     gene            19654..19727
                     /locus_tag="ACKB38_000600"
     tRNA            19654..19727
                     /locus_tag="ACKB38_000600"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19688..19690,aa:Asn,seq:gtt)
     gene            complement(19768..20310)
                     /locus_tag="ACKB38_000601"
     CDS             complement(19768..20310)
                     /locus_tag="ACKB38_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGL7979416.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20489..22091
                     /locus_tag="ACKB38_000602"
                     /pseudo
     CDS             20489..22091
                     /locus_tag="ACKB38_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(22121..24673)
                     /locus_tag="ACKB38_000603"
     CDS             complement(22121..24673)
                     /locus_tag="ACKB38_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGL7979417.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQSIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPMVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24735..26357)
                     /locus_tag="ACKB38_000604"
     CDS             complement(24735..26357)
                     /locus_tag="ACKB38_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7979418.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26563..27588
                     /gene="trpS"
                     /locus_tag="ACKB38_000605"
     CDS             26563..27588
                     /gene="trpS"
                     /locus_tag="ACKB38_000605"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGL7979419.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27740..29218
                     /gene="guaB"
                     /locus_tag="ACKB38_000606"
     CDS             27740..29218
                     /gene="guaB"
                     /locus_tag="ACKB38_000606"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGL7979420.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29269..30366)
                     /gene="recF"
                     /locus_tag="ACKB38_000607"
     CDS             complement(29269..30366)
                     /gene="recF"
                     /locus_tag="ACKB38_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGL7979421.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(30369..30737)
                     /gene="yaaA"
                     /locus_tag="ACKB38_000608"
     CDS             complement(30369..30737)
                     /gene="yaaA"
                     /locus_tag="ACKB38_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGL7979422.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30896..32146
                     /locus_tag="ACKB38_000609"
     CDS             30896..32146
                     /locus_tag="ACKB38_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGL7979423.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32143..33426
                     /locus_tag="ACKB38_000610"
     CDS             32143..33426
                     /locus_tag="ACKB38_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGL7979424.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGEIIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33468..34289
                     /gene="rodZ"
                     /locus_tag="ACKB38_000611"
     CDS             33468..34289
                     /gene="rodZ"
                     /locus_tag="ACKB38_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGL7979425.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34300..34845
                     /gene="pgsA"
                     /locus_tag="ACKB38_000612"
     CDS             34300..34845
                     /gene="pgsA"
                     /locus_tag="ACKB38_000612"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGL7979426.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34842..35669
                     /locus_tag="ACKB38_000613"
     CDS             34842..35669
                     /locus_tag="ACKB38_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGL7979427.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35654..36493
                     /locus_tag="ACKB38_000614"
     CDS             35654..36493
                     /locus_tag="ACKB38_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGL7979428.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36486..37280
                     /locus_tag="ACKB38_000615"
     CDS             36486..37280
                     /locus_tag="ACKB38_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGL7979429.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37339..38157
                     /gene="mreC"
                     /locus_tag="ACKB38_000616"
     CDS             37339..38157
                     /gene="mreC"
                     /locus_tag="ACKB38_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGL7979430.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAITNGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38157..38651
                     /gene="mreD"
                     /locus_tag="ACKB38_000617"
     CDS             38157..38651
                     /gene="mreD"
                     /locus_tag="ACKB38_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGL7979431.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38745..39923
                     /gene="pcsB"
                     /locus_tag="ACKB38_000618"
     CDS             38745..39923
                     /gene="pcsB"
                     /locus_tag="ACKB38_000618"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGL7979432.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40147..40926
                     /gene="rpsB"
                     /locus_tag="ACKB38_000619"
     CDS             40147..40926
                     /gene="rpsB"
                     /locus_tag="ACKB38_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGL7979433.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            41005..42045
                     /gene="tsf"
                     /locus_tag="ACKB38_000620"
     CDS             41005..42045
                     /gene="tsf"
                     /locus_tag="ACKB38_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGL7979434.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42291..42614
                     /gene="tnpB"
                     /locus_tag="ACKB38_000621"
     CDS             42291..42614
                     /gene="tnpB"
                     /locus_tag="ACKB38_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGL7979435.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT        13416 a         7389 c         7887 g        14137 t
ORIGIN      
        1 gctccagcct ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg
       61 atagtgcatg agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt
      121 taatttgtgt actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag
      181 tttcacgatt tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac
      241 gaacctcttc atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg
      301 caccaagcat agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt
      361 actctgtaac cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg
      421 tggtttggac attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt
      481 gctctttcat atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt
      541 atccgcctgc acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca
      601 agactagacc aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt
      661 gtgaaactgt cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa
      721 tatctgaagc atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca
      781 agacattctc taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat
      841 caacatcttc tgctaaaggc ttattcttag cgtgaacttt caagattgct tcacgacctt
      901 taacatcagg acgaccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag
      961 ggtcaagtac atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa
     1021 aaccatccat ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc
     1081 cgagaccgac tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg
     1141 gtgctgcttt tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa
     1201 acatttctac aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga
     1261 ctgccttagc aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg
     1321 gaatacgggc tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa
     1381 ctagttcttg tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt
     1441 ctttatttgc tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc
     1501 ctcccatatt tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg
     1561 atacgagtag attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt
     1621 tatggtcagt agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc
     1681 tggtaaattt ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt
     1741 tacttgtttt aggattttta tagacaccag aaacttcgat aacactacca tttggttggt
     1801 aagttaattc ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt
     1861 gctgacttcc tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa
     1921 agataaataa taaccataga aaaggatttt taattaaacc attattttgt tttttcatta
     1981 aagattattc tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat
     2041 tacgataatt ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa
     2101 agcaagtata gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg
     2161 caattttaac agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt
     2221 gacctgtatc aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca
     2281 catcttgttt aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca
     2341 tcatgaagtc catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa
     2401 tagatccttt taaaatccca actaagattg gattttttcc tgcatagtct ttagttaatt
     2461 gagcacctag ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa
     2521 tatcgttttc taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta
     2581 tatcattttt cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt
     2641 caccaaattg ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa
     2701 taaataaacg tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc
     2761 gatgacgaat gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt
     2821 ctaatggaag tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta
     2881 acacaagttc atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt
     2941 ggtactcttt tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt
     3001 ttaaaatctg ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat
     3061 tcagataagt ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta
     3121 aatcttccac attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt
     3181 cattgccaat gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta
     3241 agtaagaatt tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa
     3301 agtgaaaaat tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta
     3361 tctctccgac tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa
     3421 tcaagcgcat aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg
     3481 tcgcacctgt ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg
     3541 cttctgaaaa ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca
     3601 acttccttaa ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag
     3661 ctagaatcaa ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca
     3721 tggaatctaa tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct
     3781 tcttgagaaa atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt
     3841 ggctatctta gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg
     3901 agaatctgca tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg
     3961 agctacttta acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa
     4021 agactctagc acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt
     4081 agaagaaatc aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga
     4141 tttgaatgtg gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt
     4201 atcagattct tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc
     4261 ttttttagga agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc
     4321 agatacgtat tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt
     4381 ataatagaga taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat
     4441 accagcttct tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata
     4501 tttttcagat aacatttctt gaactttact catccgatta tcttcttcag aagttgattc
     4561 tttagctacc catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt
     4621 cttgtcgatt gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata
     4681 aggactattg tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac
     4741 ctctgattga tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt
     4801 aaatactgga attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg
     4861 agtcaatttc ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata
     4921 aaccatggga gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta
     4981 ataaatttct ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt
     5041 caaaaaactt ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt
     5101 cttgtaaacg caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac
     5161 tcaaaaattg ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca
     5221 ggaatcgtat aaactttttc cctcgactta gaataatagt acttcgctcg tgtatattta
     5281 gcagcataat cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca
     5341 ctcaaagttt gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc
     5401 tgataactct gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat
     5461 acccctccca taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg
     5521 tattcatttt gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac
     5581 ccgtgtttca ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt
     5641 catctctagt acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt
     5701 aactttcaag tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc
     5761 tgctacttcc tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc
     5821 taatctcatc cttattacct caatttatta ttgtaccatt tttatctatt ttatagaaga
     5881 aaaatatcaa atggaatttt cttccttaga ctcttttatc tctaataaac tttctccaaa
     5941 aatcagcaaa ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc
     6001 cattaatccc ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa
     6061 ataatcttga gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt
     6121 atcttttctt tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat
     6181 ctctaacaga taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg
     6241 taactcttca taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt
     6301 atgtcgttga tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc
     6361 agcattccct ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc
     6421 ctctaataac tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt
     6481 tcctaaaaga tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga
     6541 gcccaattct gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga
     6601 tttttctgga cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc
     6661 tcttaactga tataaggttg acaagcccat atggtctgca ttttcaataa ataaagtatt
     6721 agcatttgga atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg
     6781 tccctcaata aagtctaata gagtattttc caactggact tcactcattc gaccatgaac
     6841 atatccaatc gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt
     6901 gtcaactttg ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc
     6961 atcacgaatg acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt
     7021 cggcggagtt tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg
     7081 agggattggc gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt
     7141 ttccttatgc ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc
     7201 aaacacaaca tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt
     7261 tttcaatttt tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc
     7321 aatattaact gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa
     7381 aaccgtcgtc ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc
     7441 acgcatagca acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg
     7501 ctgagaagcc tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc
     7561 aacataaggg aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa
     7621 acccttcaac tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac
     7681 ctggttctta accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt
     7741 tggagcttta ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat
     7801 agaaatttga tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat
     7861 ttcaatggtt tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc
     7921 ccctttttca agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg
     7981 aaaacgacgc tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc
     8041 atctacaaaa tgaaaaccat gtctgagatt accctctatt aagtttacag attctttaca
     8101 gatatttgtc ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa
     8161 tgttttactt cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata
     8221 tcgttcaatt tcttctttta gaaaagaaaa ctgattgaaa aattcctgca taggatattg
     8281 attgaattga taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa
     8341 aaaggtcact ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc
     8401 agaaaatgct ttactattct gtaattcttc tgtaaagtac tgcgctaagt ctctttcaaa
     8461 gacttcatac tgattcatca atttttgata atcatcaaag aatattggag tatctttttc
     8521 aatataatca aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagagtc
     8581 tgcatgactt tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat
     8641 aggtgataaa gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt
     8701 ttctctcaaa agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga
     8761 taattgtgtt tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat
     8821 tcgacaaggt tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa
     8881 ttcgccctga gtttgtactt gagtaacttt tcgatagcca ttttccttta actgatggat
     8941 aaacgcgtgt tgatcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc
     9001 attgggagac ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt
     9061 agatgaatca gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga
     9121 catcaaaaac tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc
     9181 caagatagaa ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa
     9241 cacaatccta tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga
     9301 taaaccaagt attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg
     9361 atcattttct gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt
     9421 tttctcaaaa tttttctctt gtaaatagta gtttacagaa tcgtcaactt tgtcaacaga
     9481 ctgtaaaata ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga
     9541 cataccattt ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat
     9601 atgttgaata atagacttga taccattatg accacctgct gagccttttg ctcttaaacg
     9661 aattttccca acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa
     9721 accatagtaa gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg
     9781 tttaaccaga taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat
     9841 cttatcgtgt gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc
     9901 aacattgtgt tttgtttcaa aatatttatc ccctggattt cccaagccta caagtaattt
     9961 ggtcatttat ttttcctttc aaaagccaaa agggttggaa tttttttcca acctaattga
    10021 caccatttat taatttttag acattaaagc ggaattccat gatatcgcca tcttgaacga
    10081 tatattcttt tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt
    10141 atttcactag atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg
    10201 agtggataat accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa
    10261 cttctttttc accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg
    10321 tcaacttatc tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt
    10381 catcattcaa ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat
    10441 tttctgtcgc tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa
    10501 ccacatcctc gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac
    10561 ctttgacaac cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt
    10621 ctaggactgg tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt
    10681 gcgtacgtgc catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa
    10741 gaatcaattc cagattaatg gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac
    10801 gtccttgctc gcgcattaca ttttcatcat caaaagcacg aactacgtga acaatcgcat
    10861 ctacttcacg aatattggcc aagaatttat tccctagccc ctctcctttt gaagctcctt
    10921 ttacaatccc tgcaatatct gtaaattcaa atgttgtggg aactgtcttt ttaggagtta
    10981 tcatttcagt tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat
    11041 caatcgtcgc aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata
    11101 gtgttgattt accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca
    11161 ttctccgttt tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc
    11221 tctaactaag ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc
    11281 catcatgaca acatgctcac aattactaca ttttattttg atatctgctc ctacacgtgt
    11341 aatttcccaa cgattagcct ttttaccagt cgacttgatt gtacaagcgt gtgatttttt
    11401 catctcaaca aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc
    11461 ataaaagagg cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga
    11521 gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga
    11581 taaagctaat agtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg
    11641 ggttgaaact aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta
    11701 ctttaccaac ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt
    11761 tcacagtacc attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta
    11821 agtttaccac attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat
    11881 caggatagtt tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga
    11941 tttggttatt ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa
    12001 attcgcgtag agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag
    12061 tcaatttttt ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt
    12121 gactcaatac gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa
    12181 aggctgtttc attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg
    12241 ctgaaatttc ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg
    12301 atttgccact ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagttacat
    12361 caggtaaact agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag
    12421 aagtaattaa caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt
    12481 gaccatttga accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata
    12541 aaataggaat ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa
    12601 ataaattttt attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta
    12661 gaaagataat agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc
    12721 atatcaagtt ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt
    12781 atccacaaat tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc
    12841 ttgatcaatc aaagatttta ttttggcatg ggcatgaatg actgtggtat gatcttttcc
    12901 cccaaattcc ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat
    12961 ggctacttga cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat
    13021 actaacacca taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat
    13081 ttggctaaca tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc
    13141 cttgattttt tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg
    13201 aacatttgaa tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc
    13261 taaatgttcc gtcttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggggtgat
    13321 agtttgtgtc aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg
    13381 ttttggacta cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa
    13441 ggtattgaaa aattcttcct gagttgcgac tttttttccg ctgagtgact ggatatcatc
    13501 gattaacaaa agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct
    13561 taggtgatca agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt
    13621 aggaatattt tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc
    13681 tggtcctcca tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa
    13741 agcggctgat acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt
    13801 ttcttttaat cccgtatctg aataaggaat agatactaac tttggactat agttataaag
    13861 agttaaattt gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat
    13921 atagtgggga gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt
    13981 tagttgtttt tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt
    14041 ttcctctacc ttgatgagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag
    14101 tctttcttgt gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc
    14161 ctcctttact atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt
    14221 ccacaagctg tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa
    14281 aagaagaaat tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga
    14341 gataaaaaaa taaaagaaca gccttatttc aggctgttga taattctact atattcttct
    14401 tgatttgaaa aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca
    14461 tctaatccga gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt
    14521 tgctgtttct tttgtttttt ctctgtcaga agagcttcta atttccttac agaaatatct
    14581 tcttctataa tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa
    14641 cgcgcatggg cttgtgatag tttgccattt tctacttctg aaagaatctg ttctggcaag
    14701 gaaagtaaac gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt
    14761 tcagcatggg tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg
    14821 tttaaatttt ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa
    14881 atctgtttaa caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt
    14941 ctctctcctg caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga
    15001 atgaccccat tttctttgat agactgtgct agttcatcta gtttttctct atcaaattct
    15061 tttcggggtt gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc
    15121 atgattctac actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat
    15181 taagattcta aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg
    15241 ttacgatagt aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt
    15301 aagtctgttg atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca
    15361 aggtgaccat tggcaggcat attactttgt accgaacgaa caattacacc agatgtaaca
    15421 ttacttggaa tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc
    15481 tggattccca aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg
    15541 atagcatcat ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta
    15601 gcaattttac ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca
    15661 gagttacctg ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca
    15721 tcttccgatt ttaaggatac atttctattg agactggata cgataccttg agtgacagta
    15781 tttgcatatt cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac
    15841 ttactagaat caccaaactc agctactgtt gtcacttttt ctgaagagat tttgacgaca
    15901 gcaatatcag agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac
    15961 aatcgaatat ctactttgct ggcgccatta ataacgtgat tgttggtgac gatgtaagct
    16021 tctttatcat tctttttata aataactcca gatccttcac tagagattcg ctgagaatct
    16081 gtgtcagtat catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca
    16141 gaaacaacag catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag
    16201 gcagtttgtg taatagtact attgttgtta gagttgttta cactactttt ttgagttagt
    16261 tgagttattg aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat
    16321 aattgaaacc attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg
    16381 aattactgaa agtataaact gactagctta attataactt aaacacaaaa gttttacaca
    16441 aactgtggat aactcttttg aaattgtgat tttcttaatt gaaatctatt ttttattttg
    16501 tgaataagat gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt
    16561 aacagttggg aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg
    16621 aatttctaga tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa
    16681 ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa
    16741 aattgctgac cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga
    16801 agaatttagt aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat
    16861 tattggggga agtttaggat tatcatcatc tgtaaaaaat agagccaatc tttctgtcag
    16921 ttttggtcgc ctaaccttac cccatcagtt aatgagacta gttcttgttg aacaaatcta
    16981 tcgcgctttt acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt
    17041 gaatttttgg tagaataatt gtattaggtc tcatagctca gctggataga gcattcgcct
    17101 tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga
    17161 attttcccgg cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag
    17221 aattttatct aaaacgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga
    17281 aaaaacattt taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct
    17341 ttgaaggaaa aagacttaca aaagattaaa ggtggggaga tgaggatttc aagaatcatc
    17401 cttgattttc ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac
    17461 ttggatttgg gacagttatt gttcattttt taattattag tcacagttac cgtttaattt
    17521 gtaaaggtcg aataaataga aaagaattat acgtttttgg tgcttataca ttactaactg
    17581 aaatagtact tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat
    17641 ttttatttcc tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata
    17701 gaggactatt cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt
    17761 ccgtaatttt ctcctctata acaggagata attttgtttt acaatatcat ttcccattct
    17821 ttttcgttgt gacggtgtta acctattttg ttacattaaa aatcatttac tatttccatt
    17881 tggaactagc ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg
    17941 ctttactatt gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt
    18001 tgaatagttt tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct
    18061 ttgcaatgaa ttctcataaa gttcaaatgg agaaagagat tgctttgaag cagaagaaat
    18121 ttgaacagaa acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc
    18181 gtggttttcg acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg
    18241 gtaatttaca ggaaattgac agaatttaca atgaagtttt agtcaaagca aatcataaat
    18301 tgcgttcaga taagtacact tactttgatt tgaacaacat agaagactca gctttacgaa
    18361 gtttggttgc tcagtcaatt gtctatgctc gaaataatgg tgtagagttt acactggaag
    18421 taaaagatac gattaccaag cttccaattg aactattgga tttggttcgt atcatgagcg
    18481 ttttattgaa taatgctgtc gaaggatcgg ctgatagcta taaaaagcag atggaagtag
    18541 cagttattaa gatggaaact gaaacagtta ttgtgattca gaattcatgt aaaatgacga
    18601 tgactccttc aggagatcta tttgccttag gattctccac taagggaaga aatcgcggag
    18661 tcggattaaa taatgtgaaa gaactactag ataagtacaa caatattatt ttagaaacag
    18721 agatggaagg cagtacattt agacaaatca ttagatttaa gagggaattt gaatgaaagt
    18781 tttaatttta gaagatgtta ttgaacatca agtgagacta gagagaatat tggatgaaat
    18841 ttcgaaagaa tcgaatattc caatatcata caagacaacg ggaaaagtcc gtgaatttga
    18901 agaatacatt gaaaatgatg aagtaaatca gctttatttc ctagatatcg atattcatgg
    18961 aattgagaaa aagggatttg aagtggctca gctcattcgt cattacaatc cttacgctat
    19021 tatcgtcttt atcactagtc gatcagagtt tgcgactcta acctataaat accaggtatc
    19081 agccctagat tttgttgata aggatatcaa tgatgagatg tttaagaaga gaattgagca
    19141 aaatatcttc tacacgaaga gtatgttact tgaaaatgaa gatgttgtag attatttcga
    19201 ctacaattac aagggaaatg atttaaaaat tccttaccat gatattttgt atattgaaac
    19261 aacaggggta tctcataaat tgcgcattat tggtaagaat tttgcaaaag agttttatgg
    19321 taccatgaca gatattcagg aaaaggacaa acatactcag cgattttatt ctcctcacaa
    19381 gtcatttttg gtaaatatag gtaatatcag agaaattgat cgaaaaaact tagaaattgt
    19441 tttctatgaa gaccatcgtt gtcctatttc aagattaaaa attagaaaat taaaagatat
    19501 tttagagaaa aaatctcaaa agtgattgac aattagcaag aaattgatat aatggttata
    19561 tctgctacag atagaaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt
    19621 aacacgggtt cgaatcccgt acggactatt ttatccgcca tagctcagtt ggtagtagcg
    19681 catgactgtt aatcatgatg tcgtaggttc gagtcctact ggcggagtat agataaaagg
    19741 gaacacagct gtgttcctct ttttgtatca atttgtatca ccaagcattt tcataaggaa
    19801 gtctgttatt tcttgagaac tttctttttt tccatgtgca atccaagttt ggcagacacc
    19861 aaaaagtgca tgagttagat agatgctact atattctaat tcagtggtat ttagattcag
    19921 ttgcataaat cgcttttgta aatctgtact aagcatgata tgaagtttat ttcgtaagaa
    19981 attttggatt tctttagtcc cattttcaga aagaagggca gccagaagtg gttctgactc
    20041 tagatattca aaaacttcta aaatagcgtc tcttttgtga tgagcatgtt tttgaaaaat
    20101 atattcaaat gtatggaata gcttgctttg atagtgctca atcatatcat acttatcctt
    20161 atagtgagta tagaagctgg aacgactaat tccggctttt tctactaatt tgacagtaga
    20221 aattttatca aatggctgtt ccatcagtaa ttgtaccata gcattttcaa tagttcgctt
    20281 tgtttttaag cgtttgttac tttcttgcat atttcctcct tgtaaacaaa ttagactata
    20341 tgtctaaaaa tagatttttt atcttgtaat ttagattttt taatgtataa tctattatat
    20401 caaaatttta gacaatatgt ttaaaaaagg agaaactaag tttaaagaat ggaaagcaat
    20461 ttaaaaaaaa ccaaccttta ttattgtcat gatcgggatt tctcttattc cagatctgta
    20521 caatatcata tttttgtcat caatgtggga tccatatggg caattgtctg acttacctgt
    20581 ggcagttgta aataatgata aagaggcttc ctataatggt aatactatgg caataggaaa
    20641 agacatggtg tccaatttaa aagaaaataa aaccttggat tttcattttg tagatgaaga
    20701 ggaaggaaag aagggattgg aagatggcga ttactatatg gtagtgactt taccaagtga
    20761 tttatctgaa aaaacaacta cattatccaa tattcaatcg acagcagctt atcaatcatt
    20821 gacaagtgag caacaaactg agataagtga ttctgtatct caaaattcaa ctgatagtat
    20881 tcaatcggct cagtcaattg tagctttagt acaagattta cagggaagtt tagaaaactt
    20941 acaaaatcaa tcttctaatc tttcgacttt aaaaaatcaa tctaatcaag tatcacctat
    21001 tacttctact tctttgatag gattgtcaag tggattaaca gagatacaag gagatgttac
    21061 tagcaaatta gttcctgcca gtcagtcgat tgcatcaggt gtaaacgcat atactacagg
    21121 tgttgataaa gtttctcagg gcgcaagtca actaagtgaa aaaaatgcca ccttgacagg
    21181 tagtttggat aaactagttt caggctcaaa caccttgaca caaaaatctt ctagattgac
    21241 agcaggagtt ggttaattac aatcaggatc tgggcaatta gcagacaaat ccagtcagtt
    21301 actttcaggt gcttctccat tagagaatag agctaataaa ttggcagatg gatctgggaa
    21361 actagcagaa ggtggaacaa agttaacttc tggattggaa gatttacaga caggacttgc
    21421 ttctttagga caaggactag gtaatgctag tgatcaactc aaatcagtat caacagaatc
    21481 taaaaatgca gagattttgt caaatccact caatctttca aaaacagaca atgatcaagt
    21541 tcctgtaaat ggaatcgcaa tagctcctta tatgatatca gttgctcttt ttttgcagca
    21601 atatcaacaa atatgatatt tgcgaaattg ccttcaggac gtcatccaga gagccgttgg
    21661 gcttggttga aatcttgagc tgaaataaat ggtattatag ctgttttggc aggaattttg
    21721 gtatatggag gagttcagct tattggttta actgctaatc atgagatgag aatatttatt
    21781 ctcatcatcc taacaagttt agtattcatg tctatggtga ccactttagc aacgtggaat
    21841 agccgtatag gagctttttt ctcacttatt ttgcttttac tacagttagc atcaagtgca
    21901 ggtacttatc cacttgcttt gacaaatgat ttctttagat ctattaatcc ctggttacca
    21961 atgagctatt cagtttcggg attacgacaa acaatctcta tcaacaagtc attttcctag
    22021 ctgtcatact agttctattt actagtttag gtatgctagc ctatcaacat aagaaaatgg
    22081 aagaagatta aaaaaatcga ccgattaact ggtcgatttt ttatgcctta gatgactttc
    22141 gtctgtgatt atagattcca aatagtaaga gagaagtaaa ggaacagatt gctccagtaa
    22201 taaaaccatt gggaatgaag gaaagtgtaa tagttccttt ccccttggga atgtcaactt
    22261 tcataaatcc agtttgagct tgtttaattt ctattttctt accatcttgg taggcagacc
    22321 aacctttgtc ataaggaatg gtgaagaaaa tagatgtatc ttgttggaca tcatatgtag
    22381 caaaaacctt gtttttagaa gttgatactg tgacaggttg ttctttaatt ttttgaattg
    22441 cctcggtgaa agttttggta tctaaacgat agaaggtagg agattcaaat gatacttgtg
    22501 aatttccagg gaaactaaca ttgatattga aagttttttt ctctttagta tatcctagat
    22561 taaagaagga gaagacatta tcagttgtaa aagtcttttt ttcaccattt acaaggatgt
    22621 caaccttctt ttgtttatcg ttagaaaagt gaaggtttat gaaagagaga taaacttggc
    22681 tgttttctgg aacttcaatt tgatactgga ttgctgcatc ttcatttgaa gaacttgtga
    22741 cactaatcaa atcattagta ttttctattt tttctgtttt ttcataaggt attggagaaa
    22801 aataatcaaa attgacgtta gcaagttgat ttaaaaatga ggcctgatta tccaaggtat
    22861 gttcattgaa cttgacatca ttgtaaacag attgactcgc aactgcaatc ggaagagagt
    22921 attgattttc atatagggta agattatctt tttgatagat atctttaaag ccatacttat
    22981 caataggact gtctgagata ttgtactgga taccaaataa actatcagcc aaaatactat
    23041 tatttgcata tcggagattg agattagtcc cagaggattt aaaaccaagt ttatctaaag
    23101 tagagcttga tgaacgattt cgaacagatg aaaattgaga gattccattg tagttgaatt
    23161 tcatactgtc atttcctgtc tgagtttgta gtttttcagt acgagtaaat tgatttccaa
    23221 tatatgttga gaaagattcc atagctggga tatctcgact ataagcactt cgagaagcaa
    23281 atccccattc cttagcaatt ccgtccattt gagatgaagc atttaaactc atttcaacca
    23341 gtataaataa agagattaga atggcaaata gattcacaga tataaacttt ttgataactg
    23401 caaggagtaa aagagaatag acaaccaaaa attcaagagt aagcagaata ttcaaatctg
    23461 ttaaaaaaga ataatgcgat tttagataga tggtagctaa aaatcctgct actacaagaa
    23521 aaagcgaaac taaaaaattc cagactttaa gttctttcag acgctttaag acttctgctg
    23581 ctgtgtaaat taacaaggta gagaaaatcc aagcatagcg atgtaaaaac atgtttggag
    23641 tatgcatgcc ttgccaaaat aagtcaagag cttctatgta aaagcttgca attagaaatg
    23701 caaagaatat tacatatatg agtttcacgt gaaacttaat agatttcagc gtaaaaaata
    23761 aaatggtcaa aataaaggga aatagtccaa caaaaatcat tgggatggcc ccatactttg
    23821 ttgtgtcaaa ggaaccaatg aattgcttag caaagagatc aagataccag ctactttcag
    23881 tttgaaactt tgtaacttca gtcaattttt ccccatgtgt ctgtaaatca aatagagtgg
    23941 gaagagtcat aatcaaacta gccataccag ctaaaaagga gataactatg aaatcaagaa
    24001 cagatgattt tcgagtctta aagtcccacg aaatttgaca gagataccag aaaataagaa
    24061 acaatactgt catatatcca aaataataat tttgaataaa taagattgac agacttgtaa
    24121 agtacaatag gagtttcttt tcagttatca gtagatgtaa accagttata attaaaggaa
    24181 tcaagataaa aacatctagc caggttttta tctctaattg actgacagtg aaactcatca
    24241 gagcatagga agtagataag gctagtttta aaatctgagg gatagattga aacaatttat
    24301 tcaaactaaa aaaggttgac agaccaatca atccaaattt taagagagtt gtcagataga
    24361 tagcatctgg catattcgtt agatcaaaaa agtaaaccat aggcgcgaga aaactaccca
    24421 agtaataact agatagggca tagaagttta gccctagacc acttgtaaag gtgtaaaaca
    24481 gattactatt tccatgtagg atatttcgta aggctacatc aaaaataacg tattgatgaa
    24541 agccatctcc taatagagga gagttgtcgc tattccagta gatactttga gatagatata
    24601 ctccagacat aatcactaca ggaatgatga aagaaataaa ataggttcga tatgttttta
    24661 aaaatgattt catgttacct cgtagaatga tagaaaactc agttggttaa cccaactgag
    24721 ttttgaagtt ttatttagtc tttccaaagt tctttaactt ttgcttgtac ttctgcattt
    24781 tctaggaatt catcgtaggt ttcatcgata cggtcaatga cgccattttt agataagaca
    24841 atgatatggt tagccaaagt ttgaataaat tcgtggtcat ggctggcaaa gatgattgat
    24901 tctttaaagt ttttcaatcc atcattcaag cttgagatag attccaagtc caagtgattt
    24961 gttggatcat caagtacaag gacatttgat tttaagagca tgagttttga aagcatgaca
    25021 cgaacttttt ctccccctga caagacattt acaggtttgt taacttcatc tccagagaag
    25081 agcatacggc cgaggaagcc acgtaggaaa gtattgtcat cttcttcttt acttgcgaat
    25141 tgacgcaacc agtcaagaat tgattctcct cctgcaaaat cagctgagtt atcttttggt
    25201 aggtaagatt gactagttgt aactccccac ttgacagttc cttcatagtc aatatctccc
    25261 atgattgcac gaattaatgc agtcgtttga atatcatttt gtccaataag tgctgtctta
    25321 tcatctggac gcaagatgaa actaatatta tccaagatag tttcaccatc aatctttaca
    25381 gttaaatttt ctactgtcaa gagatcatta ccaatctcac gttccgcttt aaagttgata
    25441 aatggatatt tacgactaga tggcacaatc tcttctagct caatcttatc aagcattttc
    25501 ttacgtgatg ttgcctgcct tgacttagaa gcattggcag agaaacgagc aacaaattct
    25561 tgcaattgtt taattttttc ttctgcttta gcattacggt ctgctagcaa tttagcagca
    25621 agctcagaag attccttcca gaagtcgtag tttccgacat agagtttgat ttttccaaag
    25681 tcaaggtcgg ccatgtgagt acaaactttg tttaagaagt gacggtcgtg ggatactacg
    25741 ataactgtgt tatcaaagtc aatcaagaag tcttctaacc aagtaatcga ttggatatcc
    25801 aaaccgttag taggctcgtc caagagaaga acatctggtt taccaaaaag tgctttggcg
    25861 aggagaacct ttactttttc accgttggcc aattcgctca tgttttggta gtgtaattct
    25921 tctggaatgt ttaggttttg aagtagttga gaggcttcac tctctgcttc ccaacctcca
    25981 agttcggcaa actctccttc gagttcggca gcacgaaccc cgtcctcgtc tgagaaatct
    26041 tccttcatgt agatagcatc tttctctttc atgatgctat aaagtttttc atttcccatg
    26101 ataacgacat caatggcacg ttcatcttcg tagtcaaagt gattttgacg aagaacagag
    26161 agacgttcat ctggaccaag agagatgtga ccagtagtag gttcgatatc tccagctaaa
    26221 atttttaaaa aggttgattt tccggcacca ttagcaccga ttaatccgta agtatttcct
    26281 tctgtaaatt tgatattgac atcatcaaaa agtttgcgat cactaaaacg tagtgaaaca
    26341 tcagatactg taagcaatgt ttttctccta tatgtgtaat atatttattc tactagaaaa
    26401 tacagaaata ttcaaatttt tatttgtcaa ttttgtgtaa attatattta cagtatcctt
    26461 tacacaaatc tgtaaaaagc aaggctgatt tattttgata aattacggtt atttcattaa
    26521 aaaaatgcta taattgaaag gactatatcg aaggagaaca aaatgactaa acccattatt
    26581 ttaacaggag accgtccaac aggaaaattg catattggac attatgttgg aagtctcaaa
    26641 aatcgagtat tattacagga agaggataag tatgatatgt ttgtgttctt ggctgaccaa
    26701 caagccttga cagatcatgc caaagatcct caaaccattg tagagtctat cggaaatgtg
    26761 gctttggatt atcttgcagt tggattggat ccaaataagt caactatttt tattcaaagc
    26821 cagattccag agttggctga gttgtctatg tattatatga atctagtttc gttagcacgt
    26881 ttggagcgaa atccaacagt caagacagag atttctcaga aaggatttgg agaaagcatt
    26941 ccgacaggat tcttggtcta tccaatcgct caagcagctg atatcacagc tttcaaggct
    27001 aattatgttc ctgttgggac agatcagaaa ccaatgattg agcaaactcg tgaaattgtt
    27061 cgttctttta acaatgcata taactgtgat gtcttggtag agccggaagg tatttatcca
    27121 gaaaatgaga gagcagggcg tttgcctggt ttagatggaa atgctaaaat gtctaaatca
    27181 ctaaataatg gtatttattt agctgatgat gcggatactt tgcgtaaaaa agtaatgagt
    27241 atgtatacag atccagatca tatccgcgtt gaggatccag gtaagattga gggaaatatg
    27301 gttttccatt atctagatgt ttttggtcgt ccagaagatg ctcaagaaat tgctgatatg
    27361 aaagaacgtt atcaacgagg tggtcttggt gatgtgaaga ccaagcgtta tctacttgaa
    27421 atattagaac gtgaactggg tcctattcgt gagcgccgta ttgaatttgc taaggatatg
    27481 ggagaagttt ataatatgat tcaaaaaggt agtgaaagag cgcgtgaagt tgcgggtcaa
    27541 accctatctg aggtaaaagg ggcaatggga cttcattact ttaactaagt ttattttaca
    27601 agaaactatc ttttctatct cgaagaaaag atagtttttt atttacccct gtaacatttg
    27661 acaaattttt atagaatggt atgatagata caatataaaa agagtcaagc tcaaaaagaa
    27721 agaaaagagg aaacttcgaa tgtctaattg ggacactaaa tttttgaaaa aaggttttac
    27781 ctttgatgat gtattgctta ttccagctga aagtcatgtg ttgcctaacg atgcagattt
    27841 aacaactaaa ttggcagata atttgacttt aaatatccca attattaccg ctgccatgga
    27901 cacagttaca gagagtcaaa tggccattgc tattgctcgt gcaggcggtc tcggagttat
    27961 ccataaaaac atgtcaattg ctcaacaagc agacgaggtt cgtaaggtaa aacgttctga
    28021 aaatggagtt attattgatc cgttcttctt gacgcctgaa catacaattg ctgaagcaga
    28081 tgagcttatg ggtcgttacc gcatcagtgg tgttccagtt gttgaaacac ttgaaaatcg
    28141 taaattggtt ggtattttga caaaccgaga tcttcgtttt atttcagatt ataatcaacc
    28201 aatttcaaac catatgacta gtgaaaatct tgttactgct cctgtgggta cggatcttgc
    28261 aacggctgag agtattcttc aagagcatcg tattgaaaaa cttccgttgg tcgatgaaga
    28321 aggcagtctt tctggtttga tcactatcaa agatattgaa aaagttattg agtttccaaa
    28381 tgcggctaaa gatgagtttg gtcgtcttct agttgcaggt gcagtaggtg ttacttcaga
    28441 tacatttgaa cgtgcagagg ctctttttga ggcaggagcg gatgcgattg ttattgatac
    28501 tgcacatggt cattctgcag gtgtcttgcg taaaattgcc gagattcgtg ctcatttccc
    28561 agatcggact ttgattgctg gaaatattgc tactgctgaa ggtgcacgtg ccctttatga
    28621 agcgggtgta gacgttgtta aggttggtat tggaccaggt tctatctgta ctactcgtgt
    28681 gattgctggt gttggtgttc cgcaagtaac agctatctac gatgctgcag ctgttgcgcg
    28741 cgaatatggt aaaacgatta ttgctgacgg tgggatcaag tattctggag atattgtaaa
    28801 agcacttgct gcaggtggaa atgctgttat gcttggatct atgtttgctg gaactgatga
    28861 agctccaggc gaaactgaaa tcttccaagg acgtaaattc aagacttacc gtggtatggg
    28921 atcaattgct gctatgaaga aaggttcaag cgaccgttat ttccaaggtt ctgtcaatga
    28981 agcaaacaag cttgttccag aaggaattga aggtcgtgtt gcttataaag gagcggcagc
    29041 tgatattgtt ttccaaatga ttggtggtat tcgctctggt atgggttact gtggtgcagc
    29101 taaccttaaa gaactacacg ataatgctca atttattgaa atgtctggtg ctggtttgaa
    29161 agaaagccat cctcatgatg tgcaaattac taatgaggca ccaaattatt ctatgtaaaa
    29221 aacaatgaaa agaactccag tgaaaacagg agttctttta caatgttgtc aatttccatt
    29281 tacagcagct ttaccatcct gaatagtgaa gatacttaga ttttctggca gattttgaag
    29341 atggtctaag cttgttgttg tgataaaggt ttggattgat tgagaaatcg tttctaataa
    29401 ttttaactgt ctagtgttgt caagttcact catcacatcg tcaagcagta atataggaga
    29461 ttctgtggta atgctttcca ttaattcgat ttctgctaat tttatcgaga ggacgagact
    29521 acgatgttga ccttggcttc cgaaactagc atccatccca tttatataaa aagaaatgtc
    29581 atctcgatga ggaccgacac cagtattctt tttaaataaa tctctggatc tacttttttc
    29641 taaagcaatt ttgaaagatt cggataagtt ttgtttgtca gttatattga cagaagattg
    29701 ataggatatt gacaactctt cgatctgatt agagagttca aaatgtttct tacgcccaaa
    29761 tgattctagt ttttttatga aatctaagcg gtgattcatt acacgacatc cataatcaac
    29821 tagctgatca tctaacacag aaaggaatgt ttcatctatt ttttgagctg attttaggta
    29881 agtgtttctt tgctttagga tgtggttata attggttaag tcagataaat agattggctt
    29941 aatttgccca agttccatat caatgaattt tcgtcgaatc gaaggtgctc ctttaattag
    30001 ttgtaaatct tcaggagcaa ataagacaac attcatgtgt cctacataat ctgaaaggcg
    30061 tgcctgtttt aagtgattaa cttttgttac acgacctttt tgtgttagtt cgatttctag
    30121 gggaatggat ccagtttttt tctgaacgag acctgaaaga tgaagttgtt cctcatcaaa
    30181 atgaatgaga tttttatctg ttcgagttcg atgactacgc gttaaggcta aaaaatagat
    30241 agcctctaac atgtttgttt ttccttgtgc attacgtccc aaaaagacat tcaatttagg
    30301 attaaagtct attttcgttt ctttgtaatt acgaaaagtc ttgagagata gatgttgtag
    30361 ccacatgatt atctaccagg gaatcgtgga gcttgttggc ttttaggtga tgaagcaggt
    30421 ttcgatttgt cttttttaac tcccttattc atctttttga caagtttagc gatccgttct
    30481 ttttcaactt tatcagcttg gtattcatct tgttcttcag aagtaggttg tgtcaacaag
    30541 atgtcaatgt tcatgtcagg gatgtcaact ttatcgccaa tacgaagttt ttttccacga
    30601 cggctctcta attccctatt aaagtaaaca gaatgttcag agagaaatga tttaatagct
    30661 cctccgctat gtgtaattcc aagttctttg aatagtgctt ggagggtaat aaattcttca
    30721 aataatttgt attccataat tcacctcaat ctttggtatt ataccatatt ttcctaaaaa
    30781 tagtgagaga gacagcgaga aatgtaagcg atttttattt caaaagtgtt acagggaaca
    30841 agaaaatttc aggttttcgt gatataatag aagtctgtat ataaggaggt aaatcatgga
    30901 gttagtgcat ggaatttcaa cacattttat ccaatcaaaa aagtttaaaa caaacaaaat
    30961 taccgtgcgt tttaccgctc cattatccct tgatacgatt gcaggtcaca tgttgagtgc
    31021 aagtatgcta gagactgcta atcagatgta ccccacttct caagatttga ggagacactt
    31081 ggccagtcta tacggtacag atatgtcaac caattgtttc agaagagggc aaagccacat
    31141 tatagaattg acatttacct atgttcgtga tgagttttta agtaggaaaa acgtgctaac
    31201 ctctcagatt ttggaacttg taaaagaaac tcttttttca cccgtagtag ttgataatgg
    31261 gtttgatccg gccttatttg aaattgagaa aaaacaattg ctagcaagtt tagcagctga
    31321 tatggatgat tctttttatt ttgcacataa agaattggat aaattgtttt ttcatgatga
    31381 acgtcttcaa ttggaatata gtgatttacg aaatcgtatt ttagctgaaa ctccacaaag
    31441 ttcttattct tgtttccaag aatttttagc caatgatcga atagatttct ttttcctagg
    31501 tgattttaat gaggttgaaa ttcaaaatgt attagaatca tttggcttta aaggtcgaaa
    31561 aggagatgtg aaggttcagt attgtcaacc ttattctaat atccttcagg aaggtatggt
    31621 tcggaaaaat gtgggacaat ccattttgga attaggttat cattactgtt ctaaatatgg
    31681 tgatgagcaa catttaccca tgattgtaat gaatggttta cttggtggat ttgctcactc
    31741 taagctcttt acaaatgtcc gtgaaaatgc tggattagct tataccattt caagtgagct
    31801 tgatttattt agtggattct tgaggatgta tgctggtatc aatcgagaaa atcgtaacca
    31861 ggctcgtaaa atgatgaata atcaactgct tgatttaaaa aaaggttatt ttacagagtt
    31921 tgagttaaat cagaccaagg aaatgattcg ttggtcgttg ttactttctc aagataatca
    31981 atcttcattg attgaacgtg cttatcaaaa tgccttattt ggaaaatctt cagcagactt
    32041 taaaagttgg attgcaaagc ttgaacaaat tgacaaagat gctatttgta gagtagctaa
    32101 taatgtgaaa ctacaagcga tttactttat ggaaggaata gaatgacaaa ggttgttttt
    32161 gaagaaaaat actatccagc tgtaaaagaa atggtttatc gaactcgttt ggccaacgga
    32221 ttgacagttg ctcttttgcc taaaaaggaa tttaaagagg tttacgggag tgtcactgta
    32281 cagtttggtt cggtagatac gtttgtcaca gaagttgacg gagatgtaaa acaatatcct
    32341 ggaggaattg ctcattttct tgaacataaa ttatttgaga gagaagattc tagtgatttg
    32401 atgtcggctt ttacgagtct aggtgcagat agtaatgcct ttacaagctt tacaaaaaca
    32461 aactatcttt tttcagcaac ggattatttt ttagaaaatt tagatttact tgatgaattg
    32521 gtaacatcag cacactttac tgaagcttcc attctgacag agcaggatat tattcagcaa
    32581 gaacgagaaa tgtaccaaga tgatccagat tcgtgtttat tcttttcaac tttagcgaat
    32641 ttgtatcctg gtacaccttt agcaactgat atagttggaa gtgaggagtc catttcccaa
    32701 atcaatctaa ctaatttgca agaaaatttt acaaagtttt acaaacctgt aaacatgtct
    32761 ctgtttttag ttggtaattt tgatgtggag cgagtacagg actattttga aagcaaagaa
    32821 ctgaaagatt cagattttca ggaagtagca agagaaaagt tgtttttaca gcctgtaaag
    32881 ccaacagata gtatgagaat ggaagtatct tctcccaaac tagcgattgg agttagaggt
    32941 aagcgagaag tttctgaagc ggattgctat cgacatcata ttttattaaa attattgttt
    33001 gcaatgatgt ttggttggac ttcggatcgt tttcaaaaat gttatgaatc aggtaaaatt
    33061 gatgcgtcct tatctctgga agttgaaata acaagtcgct ttcattttgt catgttgaca
    33121 atggatacga aagagccagt tgctttgtct catcaattta ggaaggctat tcgtaatttt
    33181 acaaaggatt tagatattac agaggaacat ttagatatta tcaaaagaga gatgtttggc
    33241 gaatttttca gtagcatgaa ctctcttgaa tttattgcaa cgcaatatga tgcttttgaa
    33301 aatggtgaga taatttttga tttgccgaaa attttacagg aaattacttt agaggatgtc
    33361 cttgatgctg gacatcattt aatagatgat ggtgacatag ttgattttac aatattccca
    33421 tcgtagtaac ctattataat agacactaga aagaagggat gacaagtatg agaaaaaaaa
    33481 caattggaga ggttttacga ttagctagaa tcaatcaggg attgagttta gatgaattgc
    33541 agaaaaagac agaaatccag ttagatatgt tggaagcaat ggaagcagac gatttcgatc
    33601 aacttccaag tcctttttac acgcgttctt tcttgaaaaa atatgcatgg gctgttgagt
    33661 tagatgacca aattgttttg gatgcttatg attctgggag tatgattact tatgaggaag
    33721 tagatgttga tgaagatgag ttgacaggtc gtagacgttc aagtaagaaa aagaagaaaa
    33781 aaacatcatt tttaccttta ttttatttta tcctttttgc tttatcgatt ttaatttttg
    33841 tgacttatta tgtttggaac tatattcaaa ctcaaccaga ggagccttct ctttctaatt
    33901 acagtgtggt tcaatcaaca agttcaacta gctctgttcc ccactcctca agtagtagtt
    33961 cttctagtat agaatcagct ataagtgtat caggcgaagg aaatcatgta gaaatcgctt
    34021 ataagacaag taaggaaaca gttaaattgc aattggcagt ttcagatgtt acaagttggg
    34081 tcagtgtttc agaaagcgaa cttgagggcg gtgtaacctt atcgccaaag aagaaaagtg
    34141 cagaagcaac agttgcaact aaaagtcctg taacaattac gttaggtgtt gtaaaaggtg
    34201 ttgatttgac agtagataat cagactgttg atttatcgaa attaacagct cagactggac
    34261 aaatcactgt aacctttact aaaaattaag gaaaaacgaa tgaaaaaaga acaaattccc
    34321 aatctcttaa caataggtcg aattctcttt atacctattt ttatctttat tttaacgata
    34381 ggaaattcga tagagagtca tatagttgca gctattatct ttgctgttgc cagtattacc
    34441 gactatttag atggatattt agctcgtaaa tggaatgtgg tcagtaattt tggtaaattt
    34501 gcagatccta tggcggataa gttactagtt atgtcggctt ttattatgtt gattgagtta
    34561 ggtatggctc cggcttggat tgttgcagtg attatctgtc gtgagttagc tgtgacaggt
    34621 ttaaggcttt tattggttga aactggtgga acaattttag cagcagcaat gcctggaaaa
    34681 attaaaactt ttagtcagat gtttgccatt attttcttgc tattacattg gactttgctt
    34741 ggtcaagttc tactttatgt agccttattt ttcactatct actctggcta tgactatttc
    34801 aagggtagtg cctatgtatt taaagggaca tttggttcga aatgaaatca ataattgatg
    34861 taaaaaatct ttcttttcgc tataaagaaa atcagaacta ctacgatgtg aaggatatta
    34921 cgtttcacgt gaaacgtgga gaatggcttt cgattgtagg gcataatggt agtggtaaat
    34981 caacgacggt tcgattaatt gatggcttac tggaagcaga atccggagag attgtaattg
    35041 atggccaacg tttgactgag gaaaatgttt ggaatatacg tcgtcaaatc ggtatggttt
    35101 ttcaaaatcc agacaatcaa tttgttggag cgactgttga agatgatgtt gcttttggtt
    35161 tggaaaatca gggactttct cgtcaagaaa tgaaaaagag agtggaagaa gctctggctt
    35221 tagttggcat gttggacttt aaaaagagag agccagcgcg tctatcaggt ggccaaaagc
    35281 aacgtgtggc cattgcaggt gttgtagccc taagaccagc tattttaatc ttagatgaag
    35341 caacgagtat gttggatcct gaggggcgta gagaacttat tgggacagta aaaggaattc
    35401 gaaaagacta tgatatgacg gtcatttcta ttacccatga tttggaagaa gtcgccatga
    35461 gtgatcgcgt attggtcatg aaaaaagggg aaattgaatc aactagtagt ccaagggagc
    35521 ttttctctcg aaatgattta gatcaaattg gattagacga tccttttgct aatcaattaa
    35581 aaaaatcttt gagccagaat ggctatgatt tacctgaaaa ttatttgaca gaaagtgagc
    35641 tagaggataa gctatgggaa ttgctctaga aaatgtgaat tttacatatc aagaaggtac
    35701 tcccttagct tcagcagctt tgtcggatgt ttctttgacg attgaagatg gctcttatac
    35761 agctttaatt gggcacacag gtagtggtaa atcaactatt ttacaactct taaatggttt
    35821 attggtgcca agtcaaggga gtgtgagggt ttttgatacc ttaatcacct cgacttctaa
    35881 aaataaagat attcgtcaaa ttagaaaaca ggttggcttg gtatttcagt ttgctgaaaa
    35941 tcagattttt gaagaaacgg ttttgaagga cgttgctttt ggaccgcaaa attttggagt
    36001 ttctgaagaa gatgctgtga agactgcgcg tgagaaactg gctctggttg gaattgatga
    36061 atcacttttt gatcgtagtc cgtttgagct gtcaggggga caaatgagac gtgttgccat
    36121 tgcaggcata cttgccatgg agccagctat attagtctta gatgagccaa cagctggtct
    36181 agatcctcta gggagaaaag agttgatgac cctgttcaaa aaactccacc agtcagggat
    36241 gaccatcgtc ttggtaacgc atttgatgga tgatgttgct gaatatgcga atcaagtcta
    36301 tgtaatggaa aagggacgtt tagtaaaggg gggcaaacca agtgatgtct ttcaagacgt
    36361 tgtttttatg gaagaagttc agttgggagt acctaaaatt acggcctttt gtaaacgatt
    36421 ggctgataga ggcgtgtcat ttaaacgatt accgattaag atagaggagt tcaaggagtc
    36481 gctaaatgga tagtatgatt ttagggcgtt atatcccagg ggattcgatt gttcaccgat
    36541 tggatccacg tagcaaattg cttgctatga tgctactgat tttgattgtg ttttgggcta
    36601 ataacccctt gacgaatctg attcttttta tagcgacagg gatatttatt gccttgtcag
    36661 gagtatctct ttcatttttt attcagggct tgaagtctat gtttttcttg attgccttca
    36721 caactatttt tcaactattt ttcatttcta atgggaatgt tttatttgag ttttcgtttg
    36781 tgagaatcac ggattatgct ttgcaacaag ctgggattat tttttgtcgc tttgtattga
    36841 ttattttctt ttcaactttg ttaaccttaa caaccatgcc tttaagtttg gcatcagctg
    36901 tcgaagcttt attagcacct ttaaagcgtg tgaaagttcc agttcatgaa attggattga
    36961 tgctgtctat gagtttgcgt tttgtcccaa ccttgatgga tgatacgact cggattatga
    37021 atgcacagaa agctcgtgga gtggattttg gagaaggaag catcgttcaa aaagtaaagg
    37081 cgatgattcc cattttgatt cctctttttg cgacaagttt aaaacgtgca gattccttgg
    37141 ctatcgctat ggaagcgcgt ggctatcagg gtggaaaagg cagaagtcaa taccgacaat
    37201 tgaaatggac tctaaaggat acgctgacca ttcttgttat tctcgtactt ggttgttgtt
    37261 tatttttctt aaaatcttag tagctttcag gaattgataa aaagttactg taacagtttt
    37321 tgatataaag gtagggatat gaaccgtttt aaaaaatcaa aatatgtcat tattgttttt
    37381 gtcactgttc tgcttgtgtc agctctctta gcgacgactt attcaagtac aattgtgaca
    37441 aaattaggag atggaatctc attggttgat agagttgtac aaaaaccttt tcagtggttt
    37501 gattctgtca aatcagattt ggctcatttg acacgaacat ataatgaaaa tgaaagtttg
    37561 aagaaacagc tttaccaatt agaagttaaa tcaaatgagg tggaaagttt aaagacagaa
    37621 aatgaacaac tgcgccaatt gcttgatatg aagtctaaat tgcaagccac aaagacttta
    37681 gcagcagatg ttattatgcg ttctccggta tcttggaagc aggagttgac cttagatgca
    37741 ggtagatcaa aaggtgcttc tgagaacatg ttagctatta caaatggtgg cttgattggg
    37801 agtgtttcaa aagtagagga gaactctact atagtcaacc ttctgacaaa tacggaaaat
    37861 gctgataaga tttctgttaa aattcaacat ggctctacta caatttatgg aattattatt
    37921 ggctatgaca aggaaaatga cgttcttaaa attagccaat taaatagtaa tagcgatatt
    37981 agtgcgggag ataaggtgac tactggtgga ttaggaaact ttaacgttgc tgatattcct
    38041 gttggtgaag tggttgccac aacgcatagt acagactatt tgacacgaga agtaactgtt
    38101 aaattgagtg cagatactca taatgtagat gtgatagaat tagtggggaa ttcataatga
    38161 gacagttgaa gcgagttgga gtatttttat tgcttccttt ctttgttcta attgacgccc
    38221 atattagcca gcttctgggc tcatttttcc cccatgtaca tttggctagt cattttcttt
    38281 ttctatttct cttatttgag acgatagaag tatcagagta tctctaccta gtctattgtt
    38341 ttgttatagg cttggtttat gatgtttact ttttccatct aatagggatt acaactctct
    38401 tatttatctt attgggagcc ttccttcata aattgaatag tgttattttg ttgaatcgtt
    38461 ggacaagaat gctagctatg attgtgctga cattcctgtt tgaaatgggt agttatcttt
    38521 tggcttttat ggtagggttg acagtagata gcatgtcgat ttttatagtc tatagcttgg
    38581 taccgacgat gattttaaat tttttatgga ttactgtttt tcaatttatt tttgaaaaat
    38641 attatctata agaacgacat ataaatgtaa caaaggcgta atatttatta ggcctttttt
    38701 tggtatacta gtattgtctt taaaagaagg agtatctacg taatatgaag aaaaaaatct
    38761 tagcgtcact tttattaagt acagtaatgg tttctcaagt agctgtttta acaactgcgc
    38821 atgcagaaac gactgatgac aaaattgctg ctcaagataa taaaattagt aacttaacag
    38881 cacaacaaca agaagcccaa aaacaagttg accaaattca ggagcaagta tcagctattc
    38941 aagctgagca gtctaacttg caagctgaaa atgatagatt acaagcagaa tctaagaaac
    39001 tcgagggtga gattacagaa ctttctaaaa acattgtttc tcgtaaccaa tcgttggaaa
    39061 aacaagctcg tagtgctcaa acaaatggag ccgtaactag ctatatcaat accattgtaa
    39121 actcaaaatc aattacagaa gctatttcac gtgttgctgc aatgagtgaa atcgtatctg
    39181 caaacaacaa aatgttagaa caacaaaagg cagataaaaa agctatttct gaaaaacaag
    39241 tagcaaataa tgatgctatc aatactgtaa ttgctaatca acaaaaattg gctgatgatg
    39301 ctcaagcatt gactacgaaa caggcagaac taaaagctgc tgaattaagt cttgctgctg
    39361 agaaagcgac agctgaaggg gaaaaagcaa gtctattaga gcaaaaagca gcagctgagg
    39421 cagaggctcg tgcagctgcg gtagcagaag cagcttataa agaaaaacga gctagccaac
    39481 aacaatcagt acttgcttca gcaaacacta acttaacagc tcaagtgcaa gcagtatctg
    39541 aatctgcagc agcacctgtc cgtgcaaaag ttcgtccaac atacagtaca aacgcttcaa
    39601 gttatccaat tggagaatgt acatggggag taaaaacatt ggcaccttgg gctggagact
    39661 actggggtaa tggagcacag tgggctacaa gtgcagcagc agcaggtttc cgtacaggtt
    39721 caacacctca agttggagca attgcatgtt ggaatgatgg tggatatggt cacgtagcgg
    39781 ttgttacagc tgttgaatca acaacacgta tccaagtatc agaatcaaat tatgcaggta
    39841 atcgtacaat tggaaatcac cgtggatggt tcaatccaac aacaacttct gaaggttttg
    39901 ttacatatat ttatgcagat taatttacag agggactcga atagagccct cttttcaggt
    39961 tttaccgtga caatccctat taaaaattat atcaaaatag cttgaaaata ttggaaaagt
    40021 atggtagaat gaaaattgtc gtgtgaacga taatactcat tcttgatgaa ttgtgaagca
    40081 gttgcccttg ggtcgttttg cgagttgaag tcaagaagag gaaaaaaaca aaaaggagaa
    40141 atactcatgg cagtaatttc aatgaaacaa cttcttgagg ctggtgtaca ctttggtcac
    40201 caaactcgtc gctggaatcc taagatggct aagtacatct ttactgaacg taacggaatc
    40261 cacgttatcg acttgcaaca aactgtaaaa tacgctgacc aagcatacga cttcatgcgt
    40321 gatgcagcag ctaacgatgc agttgtattg ttcgttggta ctaagaaaca agcagctgat
    40381 gcagttgctg aagaagcagt acgttcaggt caatacttca tcaaccaccg ttggttgggt
    40441 ggaactctta caaactgggg aacaatccaa aaacgtatcg ctcgtttgaa agaaattaaa
    40501 cgtatggaag aagatggaac tttcgaagtt cttcctaaga aagaagttgc acttcttaac
    40561 aaacaacgtg cgcgtcttga aaaattcttg ggcggtatcg aagatatgcc tcgtatccca
    40621 gatgtgatgt acgtagttga cccacataaa gagcaaatcg ctgttaaaga agctaaaaaa
    40681 ttgggaatcc cagttgtagc gatggttgac accaatactg atccagatga tatcgatgta
    40741 atcatcccag ctaacgatga cgctatccgt gctgttaaat tgatcacagc taaattggct
    40801 gacgctatta tcgaaggacg tcaaggtgag gatgcagtag cagttgaagc agaatttgca
    40861 gctttagaaa ctcaagcaga ttcaattgaa gaaatcgttg aagttgtaga aggcgacaac
    40921 gcttaattta tacaaatagt aattacctag gagggcgggg cttagcccgg ctctcctatt
    40981 ttcaaaaaat ataggagaat taaaatggca gaaattacag ctaaacttgt aaaagagttg
    41041 cgtgaaaaat ctggtgccgg tgttatggac gctaaaaaag cgcttgtaga aacagacggt
    41101 gacatcgaaa aagcgattga attgcttcgt gaaaaaggta tggctaaggc agctaagaaa
    41161 gctgaccgtg ttgctgcaga aggtttgact ggtgtttatg ttaacggtaa tgttgcagca
    41221 gttattgaag taaacgctga aactgacttc gttgcaaaaa acgctcaatt cgttgaattg
    41281 gtaaatacta cagctaaagt cattgctgaa ggaaaacctg ctaacaacga agaagctctt
    41341 gctttgataa tgccttcagg tgaaactctt gaagctgcat acgtatctgc aacagcaact
    41401 atcggagaga aaatctcatt ccgtcgcttt gcattgattg aaaaaacaga cgcacaacac
    41461 tttggagcat accaacataa cggtggacgt atcggtgtta tttcagttgt tgaaggtgga
    41521 gacgaagcac ttgctaaaca attgtcaatg cacatcgcag cgatgaaacc aacagttctt
    41581 tcttacaaag aattggatga gcaattcgtt aaagatgagt tggcacaatt gaatcacgtt
    41641 atcgaccaag acaacgaaag ccgtgcaatg gttaataaac cagctcttcc acacttgaag
    41701 tatggatcaa aagctcaatt aactgatgat gttattgctc aagctgaagc tgacatcaaa
    41761 gctgaattgg ctgcagaagg caaaccagaa aaaatctggg acaaaattat tccaggtaaa
    41821 atggatcgct tcatgcttga taacactaaa gttgaccaag cttacacact tcttgcacaa
    41881 gtttacatca tggatgacag caagacagtt gaagcatacc ttgaatcagt taacgcttcg
    41941 gtagttgagt ttgctcgctt tgaagttggt gaaggtatcg agaaagctgc aaacgacttc
    42001 gaagctgaag ttgcagctac aatggcagca gccttgaata actaataata gaaaggaagc
    42061 aaattgcttc cttttttgta tagggcttag atttttcctg aaattgtttt ttccccagtc
    42121 tcactcgatt agaataatta aaacaaaaag ccctataata agggctaagt gcatttagga
    42181 gactatactt caaattcata gtaagcgcgt cataacaagg tatctatcct aattccccaa
    42241 cttccagtat accagataaa aaataattat cggaggaaag gtattgtact atgataattc
    42301 aactaagtga tttaggtcaa gttcaccttg tttgtggcaa gacagatatg aggcagggaa
    42361 tagactcatt agcctatgta gttaaaaccc actttgaatt ggatcctttc tccgatcaag
    42421 tctttctctt ttgtggtgga cgtaaagacc gctttaaagt tctttactgg gatggtcaag
    42481 gattttggat attatataaa cgctttgaga acggaaaact gacttggtcc agtacagaaa
    42541 aggatgtcaa agctctcgca cctgaacaag tagattggct gatgaagggc ttttctatca
    42601 ctccccaaat atagtggatt taaactagaa tagtacacct ctgcttctaa aacattgtta
    42661 gaaatcgatt tgactatcct gttcttattt cattttacta taaattcatc agaaagtcgt
    42721 gatttctatt gaaatgagga ctttcttttt atactcatct gttttcaaaa agcattctag
    42781 tccgtctccg attaacgatg gactttatca cctccttctc cagtccttg
//
LOCUS       DBDESG010000012        39664 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.012, whole genome shotgun sequence.
ACCESSION   DBDESG010000012 DBDESG010000000
VERSION     DBDESG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.012"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(55..468)
                     /gene="ndk"
                     /locus_tag="ACKB38_000622"
     CDS             complement(55..468)
                     /gene="ndk"
                     /locus_tag="ACKB38_000622"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGL7979436.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(579..4256)
                     /gene="rpoC"
                     /locus_tag="ACKB38_000623"
     CDS             complement(579..4256)
                     /gene="rpoC"
                     /locus_tag="ACKB38_000623"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGL7979437.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4289..7900)
                     /gene="rpoB"
                     /locus_tag="ACKB38_000624"
     CDS             complement(4289..7900)
                     /gene="rpoB"
                     /locus_tag="ACKB38_000624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGL7979438.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8316..8386
                     /locus_tag="ACKB38_000625"
     tRNA            8316..8386
                     /locus_tag="ACKB38_000625"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8348..8350,aa:Cys,seq:gca)
     gene            complement(8584..9915)
                     /locus_tag="ACKB38_000626"
     CDS             complement(8584..9915)
                     /locus_tag="ACKB38_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGL7979439.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10144..10968)
                     /gene="endA"
                     /locus_tag="ACKB38_000627"
     CDS             complement(10144..10968)
                     /gene="endA"
                     /locus_tag="ACKB38_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGL7979440.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11007..11195)
                     /locus_tag="ACKB38_000628"
     CDS             complement(11007..11195)
                     /locus_tag="ACKB38_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGL7979441.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11188..12471)
                     /gene="murA"
                     /locus_tag="ACKB38_000629"
     CDS             complement(11188..12471)
                     /gene="murA"
                     /locus_tag="ACKB38_000629"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGL7979442.1"
                     /translation="MDKIVIQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12495..12725)
                     /locus_tag="ACKB38_000630"
     CDS             complement(12495..12725)
                     /locus_tag="ACKB38_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGL7979443.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(12783..13820)
                     /locus_tag="ACKB38_000631"
     CDS             complement(12783..13820)
                     /locus_tag="ACKB38_000631"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGL7979444.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13804..14292)
                     /gene="coaD"
                     /locus_tag="ACKB38_000632"
     CDS             complement(13804..14292)
                     /gene="coaD"
                     /locus_tag="ACKB38_000632"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGL7979445.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14282..14821)
                     /gene="rsmD"
                     /locus_tag="ACKB38_000633"
     CDS             complement(14282..14821)
                     /gene="rsmD"
                     /locus_tag="ACKB38_000633"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGL7979446.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(14886..15878)
                     /gene="asnA"
                     /locus_tag="ACKB38_000634"
     CDS             complement(14886..15878)
                     /gene="asnA"
                     /locus_tag="ACKB38_000634"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGL7979447.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(15990..16673)
                     /locus_tag="ACKB38_000635"
     CDS             complement(15990..16673)
                     /locus_tag="ACKB38_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGL7979448.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16729..17469)
                     /locus_tag="ACKB38_000636"
     CDS             complement(16729..17469)
                     /locus_tag="ACKB38_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGL7979449.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            17503..17781
                     /locus_tag="ACKB38_000637"
     CDS             17503..17781
                     /locus_tag="ACKB38_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGL7979450.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            17859..18785
                     /gene="yidC"
                     /locus_tag="ACKB38_000638"
     CDS             17859..18785
                     /gene="yidC"
                     /locus_tag="ACKB38_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGL7979451.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18860..19654)
                     /gene="pflA"
                     /locus_tag="ACKB38_000639"
     CDS             complement(18860..19654)
                     /gene="pflA"
                     /locus_tag="ACKB38_000639"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGL7979452.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(19780..21030)
                     /locus_tag="ACKB38_000640"
     CDS             complement(19780..21030)
                     /locus_tag="ACKB38_000640"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGL7979453.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYHPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEVVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21097..21924)
                     /gene="purR"
                     /locus_tag="ACKB38_000641"
     CDS             complement(21097..21924)
                     /gene="purR"
                     /locus_tag="ACKB38_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGL7979454.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22191..23132)
                     /locus_tag="ACKB38_000642"
     CDS             complement(22191..23132)
                     /locus_tag="ACKB38_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGL7979455.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(23122..24378)
                     /gene="rmuC"
                     /locus_tag="ACKB38_000643"
     CDS             complement(23122..24378)
                     /gene="rmuC"
                     /locus_tag="ACKB38_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGL7979456.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24380..25042)
                     /locus_tag="ACKB38_000644"
     CDS             complement(24380..25042)
                     /locus_tag="ACKB38_000644"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGL7979457.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25005..25661)
                     /gene="rpe"
                     /locus_tag="ACKB38_000645"
     CDS             complement(25005..25661)
                     /gene="rpe"
                     /locus_tag="ACKB38_000645"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGL7979458.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(25672..26550)
                     /gene="rsgA"
                     /locus_tag="ACKB38_000646"
     CDS             complement(25672..26550)
                     /gene="rsgA"
                     /locus_tag="ACKB38_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGL7979459.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(26552..27424)
                     /gene="rsmA"
                     /locus_tag="ACKB38_000647"
     CDS             complement(26552..27424)
                     /gene="rsmA"
                     /locus_tag="ACKB38_000647"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGL7979460.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(27555..27863)
                     /locus_tag="ACKB38_000648"
     CDS             complement(27555..27863)
                     /locus_tag="ACKB38_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979461.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(27856..28497)
                     /locus_tag="ACKB38_000649"
     CDS             complement(27856..28497)
                     /locus_tag="ACKB38_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979462.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(28499..30529)
                     /locus_tag="ACKB38_000650"
     CDS             complement(28499..30529)
                     /locus_tag="ACKB38_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGL7979463.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(30798..31697)
                     /locus_tag="ACKB38_000651"
     CDS             complement(30798..31697)
                     /locus_tag="ACKB38_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979464.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(31769..32329)
                     /gene="rnmV"
                     /locus_tag="ACKB38_000652"
     CDS             complement(31769..32329)
                     /gene="rnmV"
                     /locus_tag="ACKB38_000652"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGL7979465.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(32329..33102)
                     /locus_tag="ACKB38_000653"
     CDS             complement(32329..33102)
                     /locus_tag="ACKB38_000653"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGL7979466.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(33407..34300)
                     /locus_tag="ACKB38_000654"
     CDS             complement(33407..34300)
                     /locus_tag="ACKB38_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HGL7979467.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(34584..34718)
                     /gene="rpmH"
                     /locus_tag="ACKB38_000655"
     CDS             complement(34584..34718)
                     /gene="rpmH"
                     /locus_tag="ACKB38_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGL7979468.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(34863..36077)
                     /locus_tag="ACKB38_000656"
     CDS             complement(34863..36077)
                     /locus_tag="ACKB38_000656"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGL7979469.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(36233..36527)
                     /locus_tag="ACKB38_000657"
                     /pseudo
     CDS             complement(36233..36527)
                     /locus_tag="ACKB38_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36689..37141
                     /locus_tag="ACKB38_000658"
     CDS             36689..37141
                     /locus_tag="ACKB38_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGL7979470.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(37176..38564)
                     /locus_tag="ACKB38_000659"
     CDS             complement(37176..38564)
                     /locus_tag="ACKB38_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGL7979471.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            38630..39592
                     /locus_tag="ACKB38_000660"
     CDS             38630..39592
                     /locus_tag="ACKB38_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGL7979472.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT        11146 a         9306 c         7658 g        11554 t
ORIGIN      
        1 actatacttt ctattcaaat gagcttttaa ccaattgatt gagccaatcc actcttaaaa
       61 ccaaagagca atttctcgct tagctgactc ttctgaatct gaaccatgta caacattttg
      121 gataatctca ttttctccag cagcttttgc aaaatcacct cgaatagtgc ctggtaaagc
      181 ttcttctgga cgagttgcac ccatcatggt ccgccaagtt tcgattactt tgggaccaga
      241 aatgacaccc acaagaactg gacctgaagt catgaattca cgaatcggtg ggtaaaaact
      301 ctgaccaacc aagtcctgat agtgctggtc aatcaactct tctgaaacct gtgaacgaaa
      361 ctccaatttt tcgattgtaa atccacgttg ttcgatgcgc tttaacactt cacccactag
      421 ccctcttttt acaccatctg gtttgatgat aaagaatgtt tgttccatac ccgtctcctt
      481 tgtcagcttc tttcttttat tttaccacat ttcgtggaaa aatggagaaa gttttcagaa
      541 gagagaatga gagaaccctc gggttctctc attctctctt attctactgt ttcttccaca
      601 gtttcaacgg cagtatccac aactacttct gttgtttctt catttccttc ttcctctact
      661 ggaggattaa ggtattcttc ttcgttgaca gcatgtggtt caaggttacg gtaacgggcc
      721 ataccagtac cagctgggat gatcttaccg atgataacat tttctttaag tccaaggaga
      781 tggtctttct taccacggat agctgcgtca gtaaggacac gagttgtttc ctggaaggaa
      841 gccgctgaca agaaactgtt tgtttcaagt gaggctttgg taattcccat aaggactggg
      901 cgacctgtcg ctggaactcc acctgcgata aggacatctt tgttggcatc tgtaaagtca
      961 ttgatatcca tgagggtacc catgagaaga tctgtatcac ctggatccat gacacggact
     1021 ttacggatca tttgacgaac cattacctcg atgtgtttgt caccgatttc taccccttgg
     1081 ctacggtaaa ctttttgtac ttcaccgaga aggtacgttt caactgacaa gacatcacga
     1141 actgcaagga gacgttttgg ttggatagaa ccttctgtca gagcagcacc acgcgctact
     1201 tggcccccaa cttcgacacg catacgagct gtaaatggaa cgacatattc accttcgcca
     1261 gtttcaccct taacaaagac tttcttggta cgagttgatg catcttcttc gatagcagta
     1321 acttgtcctt taacctctgt aataaccgct tcccctttag gattgcgggc ttcaaagatt
     1381 tcttggacac gaggaagacc ctgagtgata tcggtatttg aggcaacccc acctgtgtgg
     1441 aaggtacgca ttgtaagctg tgtaccaggt tccccgatag attgggcagc gattgtacca
     1501 actgcttcac caacttcaac cgcatcacca gtcgccaagt tgataccgta acagtgacgg
     1561 cagacaccgt gacgagtgtt acatgtaaat acagaacgga tagtcacttc ttccacacca
     1621 gcattgacaa tttcacgcgc cttgtcttct gtaatcaatt catttggacc aataatcact
     1681 gcaccagttt ctggatgttt aacagttttc ttagtgtaac gaccgttgag acgctcttcg
     1741 agagactcga tcatctcttt tccttctgcg atagaacgga tcaagagacc acggtcagtt
     1801 ccacagtcgt cctcacggat gataacgtct tgggcaacgt cgaccaaacg acgagtcaag
     1861 taacctgagt cggctgtctt aagggccgta tcggtcatac ctttacgagc accgtgagtt
     1921 gagaagaaca tttccaatac cgacaaacct tcgcggaagt ttgaaaggat tggcaattcc
     1981 atgatacgtc cattcggagc agccatcaga ccacgcatac cggcaagctg tgagaagttt
     2041 gagatgttac cacgggctcc agagtccatc atcataacga ttgggttctt aggatcttgg
     2101 ttagcaatca agcgtttctc aagtttttca cgggcagcac gccattcagc tgtaacagca
     2161 ttgtaacgct cgtcgtctgt gatcatacca cgacggaatt gtttggtgat ttgttcgaca
     2221 cgtttgtgtg attcttcaat gatttcagcc ttgtcatcaa cgactgggat atcggcaata
     2281 cccactgtca atcctgcaag agttgagtgg tggtaaccga ggttcttcat gcggtcaagt
     2341 agggcagaag tttctgtcgt acggaaacgt ttgaagattt cagcgatgat atttccaagg
     2401 tttttcttct tgaatggagg gttgagctca agattgctga tagcttcctt gatatctcca
     2461 ccaagtggca agaagtattt agctggaaca ccttctgtca agttggcatt gtttggttct
     2521 tgcaagtatg gtagcccctc tggcatgata tcgttgaaga gaattttacc aactgttgta
     2581 agcaagacct tatgtctttg ctcttctgtc caaggcttgt tgaggctgtc tgttgcgata
     2641 ccaacacgtg agtggaggtg aacataacca ttgcggtaag ccataaccgc ttcgtcacgg
     2701 tctttgaaga ccattccttc accttcgcga ccagcttctt ccatggtcaa gtagtagtta
     2761 cccaaaacca tgtcctgaga tggagtaact accggtttcc catctttcgg gttcaagatg
     2821 tgctcagcag ctagcatgag gatacgagct tctgcttgtg cttcttctga aagtggtacg
     2881 tggatggcca tttggtcccc gtcaaagtca gcattgtagg cttcacagac aagtgggtgc
     2941 aagcgaagag ccttaccatc aatcaagact ggctcgaagg cttggatacc caaacggtga
     3001 agggtcggtg cgcggttcaa aagcactggg tgttctttaa tcacttcttc aaggatatcc
     3061 cagatacgct catctccgcg ttccaccaag cgtttagctg ctttgacgtt ttgcacgata
     3121 tcacgggcaa cgatttcacg catgacaaat ggtttaaaga gttcaatcgc catttcacgc
     3181 ggcacaccac attggtacat cttaagagtt ggaccaacgg cgataactga acgtcctgag
     3241 aagtcaacac gtttaccgag caagttttga cggaagcgtc cttgtttacc tttaagcatg
     3301 tggctcaatg atttcaatgg acggctacct ggtcctgtga ttggacgacc acgacgacca
     3361 ttgtcaatca aagcgtcaac tgcttcttga agcatacgct tctcattttg aacgatgata
     3421 cctggtgcat ttaactcaag caaacgagcc aaacggttgt tacggttgat aacacggcgg
     3481 taaaggtcat tcaagtcaga tgaggcaaaa cggccaccat ccaactgcaa cattggacga
     3541 agatctggtg ggataaccgg aaggatgtta agaatcatcc attcaggttt gtttccagac
     3601 ttgtaaaagg catccaaaac atccaaacga cggatggctt tgacacgctt ttgtccagta
     3661 gctgttttca attcttcttt gagttcagca atttcttttt caagatctac ttgcttcaaa
     3721 aggtcttgga tggcttccgc acccatcttg gcaacaaatg aaccataacc atattcacgc
     3781 aagcgctctc ggtattcgcg ctctgtcatg atagacttgt gctcaagtgg tgtatcctta
     3841 ggatcaatca ccacataagc cgcaaagtag ataacttcct cgagggcacg agggctcata
     3901 tcaagggtca agcccatacg gcttggaatc cccttgaagt accagatgtg agatacagga
     3961 gctttcaatt cgatatgtcc catacgctca cgacgaactt tcgtacgcgt tacttcaacc
     4021 ccacagcggt cacaaacaat tcctctgtaa cgaatgcgtt tgtacttacc acaagcacat
     4081 tcccagtctt ttgtaggacc aaagatcact tcatcaaaga gtccttcacg ttctggtttc
     4141 aaggtacgat aattgattgt ttcaggtttt ttgacttctc cataagacca tgaacggact
     4201 ttacttggag aagctagggt gatttgcata cttttaaaac gatttacatc aaccactatt
     4261 tcttcccttt ctattctaag tgaactgctt attcttgttc agcagcttct tctgttgctt
     4321 ccgcttttgt tgctttctca gcttcttcag cttcaaaggc tgctttagcc tcttgggctg
     4381 ctttttcgcg ggctttttca aggtcatcta cgtggatgac atcttcgtcc attccttcat
     4441 ccaagtcgcg aagttccact tcttggtcat cttcgtctag gacacgcatg tcaagaccaa
     4501 gagattgcaa ttctttgaca agaactcgga aggattctgg aacacctggt tttggaattg
     4561 gtttgccttt tgtaatagct tcataggctt tcaaacgtcc gttgatatcg tccgacttgt
     4621 aagtcaagat ttcttgaagg acatttgacg caccgtaggc ttcaagagcc caaacctcca
     4681 tctcaccgaa acgttgtcca ccaaactgag ccttacctcc gagtggttgt tgggtaacag
     4741 ttgagtatgg tccgactgaa cgcgcgtgca atttatcatc aaccatgtgg tggagtttga
     4801 tcatgtacat gactccgaca gaaacacggt tatcaaacgg ttcaccagta cgtccatcgt
     4861 aaaggatcgt tttggcatcg ctatccatac ctgcttcttt aacagttgac caaagatctt
     4921 cagaacttgc tccatcaaag actggtgtag cgatgtgaat accaagagta cgagctgcca
     4981 taccaaggtg aagctccata acctgaccga tattcatacg tgatggtacc ccaagtgggt
     5041 tcaacatgat gtcgactgga gttccgtctg gaaggtaagg catgtcttct acaggaacga
     5101 tacgagagac aacccctttg tttccgtgac gtccggccat tttatctccg accttaatct
     5161 tacgtttttg agcgatgtaa acacgaacca acatgttaac acctgattgc aactcatctc
     5221 catttacacg tgtaaagatc ttaacatcac gaacgacacc atcggcaccg tgtggtacac
     5281 gaagagaagt atcacgcact tcacgagact tgtctccaaa gatagcgtgc aagagacgtt
     5341 cttcagctga aagatctttc tcacccttag gtgttacttt acctacaaga atatcacctt
     5401 ctttaacctc agcaccaata cggataatcc ccatttcgtc aaggtctttg agggcatctt
     5461 caccaacgtt tggaatttcg cgagtgattt cttcaggccc aagctttgta tcgcgcgttt
     5521 ctgattcgta ttcttcaagg tgaacagatg tgtagacatc gtccttcacc aagcgttcgc
     5581 tcatgataac ggcatcctcg aagttgtaac cttcccaagt catgtaggca acgattgggt
     5641 tttgtccaag cgccatttct ccattttcca tagaaggtcc gtcagcgatg aaatcgcctt
     5701 tttcaacgac atcaccaact tttacgagag tgcgttggtt gtaagcagta cctgagtttg
     5761 aacgacggaa tttttggatg tggtaaacat ccaatgaacc atcttcacga cgaacttcta
     5821 ccttgtcagc atctgcgtaa gtaactttac catcatactg agcaatcaca gccgcaccag
     5881 aatcgtgggc tgcttggtat tccataccag taccaacgta aggtgcctga ggattaatca
     5941 atggcacagc ctgacgttgc atattggctc ccatgagggc acggttggag tcatcgtttt
     6001 ccaagaaagg aatacatgct gtcgcaacgg caactacctg ttttggtgaa acgtccatgt
     6061 agtcaacaat attagctgga tactcttggt tgaccccttg gtgacgtccc atgacaatct
     6121 tctcagcaaa ggttccatct tcattcagac gagagttagc ctgagctaca gtatattcat
     6181 cttcttcatc agctgtcaac caaacaattt cgttcgtgac aacacctgtt tcacggtcaa
     6241 ccttacggta tggtgtttga acaaaaccat atttgttcaa gtgtccataa gatgacaagt
     6301 tattgatcaa accgatgtta ggtccttcag gtgtctcgat tggacacata cgaccatagt
     6361 gagtgtagtg cacgtcacgt acttcatatc cagcacggtc acgagtcaaa ccaccaggtc
     6421 ctaaggctga caaacggcgt ttgtgagaca actcagaaag cgggttgtgt tggtccatga
     6481 actgtgacaa ctgtgatgaa ccaaagaatt ctttaactgc agctgttaca ggacggatat
     6541 tgataatttg ttgtggtgtc aagacttcat tgtcctgaac agacatacgt tcacggacat
     6601 tacgttccat acgagaaagt cccaaacgta cttggttggc aagcaattca ccaaccgcac
     6661 ggatacgacg atttccaagg tggtcgatat catctacacg gccaagtcct tcagccaagt
     6721 tgaggaagta gctcatctca gcaaggatat ctgcaggagt caccgtacga accttgtcat
     6781 ctgggttagc attaccaatg atcgttacga cgcgatctgg atcagttgga gcaataacct
     6841 tgaatttttg aagaacaaca ggctcagtca caacggctgc atcgtttggg atgtagacaa
     6901 tcttgttcaa gtcgccatcc aaatggcttt caatgctttc aatcacgcta cgagtcataa
     6961 tcgtaccagc ttctaccaag atttctccag tttcagggtc taccaatggc tctgcaatgg
     7021 tttggttgag caaacgtgtt ttaacattga gttttttatt gattttgtaa cgaccaactg
     7081 ctgccaagtc ataacgacgt gggtcaaaga agcgagctac aagcaagcta cgtgagcttt
     7141 cagccgtctt aggctcacct ggacgaaggc gttcgtaaat ttctttcaag gcttcgtctg
     7201 tacgagagtc cattggattc ttgtggatat ctttttcaac agtgttgcga accaattcgc
     7261 tgtcaccaaa gatatcaaag atttcatcat cacctgagaa accaagagca cgaaccaagg
     7321 ttgtaaatgg aatcttacga gtacggtcga tacgagtgta ggtgatatct tttgagtcgc
     7381 tttcaagttc caaccaagct ccacggttag ggataacagt tgaaccatag cccaccttac
     7441 catttttgtc tactttgtcg ttaaagtaaa cacctggtga gcggaccaac tgagaaacga
     7501 taatacgttc accaccattg atgatgaaag tacccatttc tgtcatgatt gggaaatcac
     7561 caaagaaaac ttcttgggtc ttgatttcgc ttgtttcttt attgatcaaa cggaaggtta
     7621 caaaaattgg tgctgagtag ctagcatcgt ggatacgagc ttcttctagc gtatattttg
     7681 gttccttgat ttcatatcca acaaattcca actccattgt gtctgtgaag tttgaaattg
     7741 gcaatacatc ttcaaacact tccttaagac cgtggtctag gaaagctttg aatgagtcag
     7801 tttgaatttc aatcaaattt ggtaagtcaa gaacttcttt gattcttgaa aaactacgac
     7861 gggtacgatg tttcccgtat tgaacgtcat gtcctgccaa gatgattctc ctttgtaaat
     7921 aagttccaag ccttgtcaat caggcttttc taatcgtcat atggttgtaa accccttatc
     7981 accgtgtcct cttgacgaat tttcagaatc tttaagcctc tgttacaaat gctcaaaatc
     8041 ttgaaaaaaa gcacaaaaag agcagctaaa tctgactttt tcagaagatt taactgctgt
     8101 gagccttgtc tggacaatat ttcagacaaa acctacgaca aatgattacc catattatac
     8161 cctatttagc tagatttttc aaggggtttc agtaggtttt tggtaaattt tttcccatag
     8221 aaaacttggc atcacattcg aatcacgcta tggtacaaaa aactgaaaaa actattgact
     8281 gaaaatcatt ttcaaggtat aataataaac gttaaggcgg tatagccaag tggtaaggca
     8341 cggctctgca aaagcttgat cgtcggttca aatccgtcta ccgccttcta taacttgatt
     8401 tatcaggttt caaatgaaca gaaagcccaa tttgaagggc tttttttatt ttccctcgaa
     8461 taaatacgta taactttaaa aacttttgga gcgagtttgt ggcagagttc tttccatggc
     8521 ataattccct tttgaaatca attgaaaaaa gcttcgtcac gcctggtgac aaaagcctct
     8581 agtttattct gtttcttctt ctatttcgac ttctgtgatt tggactttta ccttggtaac
     8641 acgtccattc ttcactttat cattggttaa gataaactgt ttgttttggc tgaccaattc
     8701 atagctgagt ttctcagtcg ttggaatagt tcccactcct gtcaaataat aaccagcgat
     8761 agtatcaacg tcatcacttt ccagttcaac atcaaagtaa ttattgaaat cattgagggt
     8821 catagttcct tgaacaatgt aggtatcctc gccaatctga tgaacatcta ttgctgcttt
     8881 gtccgtttca tcatcaattt caccaacaat ttcctctaag aggtcttcca aagtcaccaa
     8941 accagccata ccaccgtatt catccagcaa gattgccatt tgtctttggg tatttcgcag
     9001 ttcttttagc aagtcatcca caaaaatagt ttcaggtaca aaaagtggat cttgtaaaat
     9061 tctcttccaa acaatattgt caaaaccgtc cacaaagcct gccttaagga gactcttggt
     9121 gtgaatgatt ccaattacat tgtccttatc cccatcataa accgggatac gagaataatt
     9181 ttgttttaaa atactttgga taatggcttg actatcatcc tgaatatcca ccataaaggc
     9241 atccgttcga ggaaccataa cctctcgtgc catcagttca tcgagcgaaa agacaccttg
     9301 tagcatctca atctcatcag catccaatgt ttcttcacta tttgtcagca tataggcaat
     9361 ttcatcacgg gtcatctttt catccgcatc atcgaatgac ataggagtca aatggctcaa
     9421 gaaattggtc gaagcagcta aaagccaaac aaaaggactg actagttttc cgatcccaat
     9481 gataatcggc gctgtacgaa ttgccaaggc atcctttaga ttaagagcga ttctcttagg
     9541 atataattcc ccaaaaacga tggaaatata ggtcaaaaat gccaaggata gaaaagttgc
     9601 cacggcttgt gctgtttcgc cattcccaag ccaagaggca atcacacgtc ctagagtatc
     9661 agttaaactc gcccctgata agattgtaat cagggtgatt cctacctgga tggttgataa
     9721 aaagtggtta ggattttcta gtaccttcag caggcggatg tagcgtctgt ctccttcttc
     9781 cgccttttgt tcaactcggg cacgattaag agaaaccatg gccatttctg tggctgagaa
     9841 aaaggcattt aataccgtca agataaacaa taaaacaaat tgtagcaaca aattctgact
     9901 acttgggtct tccatagttc ttctcctaaa atagtatctt atcctttatt atatcacaaa
     9961 atttaatcca atacatatta ttttttctta ccgtaacttc tcaaagtaac ttccatctct
    10021 ctcccaaaac tggaagttag ttttagaagt tgctattttt tcttagtacc tgttctagtc
    10081 ctgcttaaac tagtaaaaag aactcctaca tccataagtg acataggagt ttaggcttat
    10141 cttttactga gttacagtta cttctccagt tcggtaatcc agttgaagtc ccttttgaac
    10201 attgggaact agaacattga attccaattc tccatccgaa gacttggctt caatctgtga
    10261 agctgaggga actaaatcct cgtttgaagc gtagtaaagg gttacacggt aacggacacg
    10321 cttgttttgg tccaaggctt tacgcacctt gctttcatag tagttttgac cagtcgaata
    10381 ctcggcttgt gcctgatttg cccaggctgt ctgaacagca atgtttttag gattgcttgt
    10441 tgaggcatca aaaccatcca aaccaccgat taaggcatag cctaacaaat gacctctatc
    10501 gactgcatgg gtataagagc cctttagatt cttgacctga tgccaacctg gaggagtcca
    10561 agaagttgaa ccattcccag tttctttacg attcttgtac tgacgagtgg ccttagacaa
    10621 gagggcatta gctacggttg gaacagtttc cttgcccact gtctttgttt tattgtcagc
    10681 gtagggctta cttgaaacct tggcatctag atttgtttta ttaccattga cgataaaagc
    10741 acctgagcca ttccactcca gactcccctt tatttgactc ttgactgcgt ctgttaagac
    10801 actctctgcc aatgcttgac taggagcttc agacgcttgt tttttctgac taagattggt
    10861 tttgggacta ttaggtgccg acggcatctg cttgatataa tagctccctg tagacaaaag
    10921 caataacact agcagtccga ttagtgtctg tcttgttttt ttgttcatat ttctccttat
    10981 ctttagaaaa aggctggttc tccagcctaa tttcctgtaa atttatgaat caattcctgc
    11041 cattttgctg gagacaggat agcccatgga tcttgaccct tgcccaagat tccataacct
    11101 accattaaac cgattcctag agccagagta cctaaaatca gtactatgat gactaaaagt
    11161 aaacgcttga ctacgtagct tgatttctta ttcatcttca tcacttgcct caatccgctg
    11221 aatcttagca cctagctgcg ccaacttctc atggaaaccg tagtaacctc tatccaagtg
    11281 aaccaattta ccgaccacag tttctccctg tgctaccaaa cctgtcaaaa tcaaggccgc
    11341 actggcacga aggtcagttg aaagaacttc tgctccctgc aaaggctgtc caccaacaat
    11401 acgagctgta tcacggataa tctcagaatg caagcccatg cggcgcatct cttctaggtg
    11461 ttggaaacga ttttcgaaaa ctgtctccac catggttgat tcgccttttg caactgtcat
    11521 caaggctgta aattgagcct gcatatctgt tggaaatcct gggtggggca aggttttcac
    11581 atgaacagct tttagatttt ctagttgaga acgaacacga attccttcgt cttcttcaat
    11641 tacttcaaca cccatttcaa gtaacttggc aatcaaggga cggttgtgct cccagacagc
    11701 gtctcgaatc aagacatcac caccagtcat ggcagcagct accataaagg ttcctgcttc
    11761 gatacggtct tggactacat tgtgagtcgt accatgaagt ttctcaacac cagtaatggt
    11821 tatagtctct gtaccagcac ctttgacctt ggctcccatt tcattaagga gaatggctaa
    11881 gtcaacaatc tcaggctcac gcgcagcatt ctcaatcact gtcaccccat cagccagagt
    11941 cgctgccatc atcaagttct gcgttgcacc aacacttgga aagtccatat agatatgagc
    12001 accatgcaag cgttctgcct tggcttcgat gtaaccagct gtctgactaa tcttaacccc
    12061 catagcttcc agacctttca aatgaagatc aataggacgg ctaccaatcg tacaaccacc
    12121 tggcatggat accttggcat gacccacacg ggcaaggatt ggccctaata caacgatgga
    12181 ggcgcgcatc ttgctgacat acttgtaagg ggcttcctca gtgatgtcgc cagtagcatc
    12241 caccttgaca agatgagctt cctcatcaaa gtcaaccttg gcattcaaac caccaactac
    12301 ctgattcata ataaagacat ccgacaaaat cggaacattc tgcaagacgg tctttccttc
    12361 acttgctaga atagtcgctg ccaacaaggg taagactgca ttttttgctc cctcgatcgt
    12421 cacgcttcct accagacgat tatcgccacc ttgaatcaca attttatcca tactcgtttc
    12481 ctttactctt gatttcataa taatcctcta agcgcttctt gccacaaatg atacaggcta
    12541 ataaagaaag aactcaggat gtagcctatg acaatgctaa aaaaggctac taataaacga
    12601 acttttcttg tattatcatc tgtcactttt aaaaccttct cccatctaac aagatccttt
    12661 aaaaggtaaa aactcacata aataaagaac atatgactac ttagagtgaa taataattga
    12721 accatttgac tattatacca tcttctctgt ttgtgcgcta gtttggaaac ttcactcaaa
    12781 aattagggat tgtttttcaa gtaatcaatc gcatcttgta gggttttaac gggcacgatt
    12841 ttcatatccg tcttgattgt tttagccgct tctagggctg tttggtagtt gtttttcgcg
    12901 tccggatgcg ccttttgttc ttcttcgcta acagggttat caggagcaaa gaaaatagca
    12961 gcacctgccc tagccgaagc tacaaccttc ttatcaatac ctccaatgtc tcccacatta
    13021 ccatcgcggt caatggtacc tgtaccggca acaatacgac cattacgaag atctgggtga
    13081 gctatttgag tatagatagc tagactaaac atgagaccag cacttggacc gccaatacca
    13141 gctgttgaaa agctaattgg gacattgctg attacctctg tacggtcaat caagccgatt
    13201 ccaattccat ttttgccatt ttccaaggtg atgatttttc cttctgcaga cttggtttgc
    13261 ccatcctctt cataggtgac cttgacggaa tcccctaatt tttgagaact gacgtaatca
    13321 atcaagtctt tggaactatc aaaggtctga tcattgactg ctgtgactgt atcagagata
    13381 ttgagaatcc ctttaaaggt tgaattatcc gtcacattca aaacataaac tccaaagtac
    13441 ttgagttcga tatccttacc agctgttttt agtccttgat acttggccat attttgcgat
    13501 gtttgcatgt agaattgatt gattcgcata aattcaacat cggaagaacc acctgtagtc
    13561 tcctgagcac tacgaatatc tgtaaaaggt gtcaaccaag cataaatcat atgagctaaa
    13621 gtggcatgtt gaacaccaac cgtaacgaat tgataggcac cagcttcctt atcttctgtg
    13681 tcatttactt taaggacttg gcgaatatct tccgaaccac ctggaacctc tatataataa
    13741 ggcaagggca ctacaaatgc caagaaagtc acaatcaagg ccgcaatgac gtataagggc
    13801 catctaatct tttttttcat ttcttatttc ctccaaaata ctctcgggaa catagcaggc
    13861 aatatcctga ccaaacttca aaagctctct aacgcctgat gaactgatat agagatgttc
    13921 aggtcgacta tgtaaataaa tagtctctat atcagaagac agctgatgat tgtagtaatc
    13981 aaaactggct tcatattgca aatccgacgc atttctcaaa cctcgcacta ggcaagtagc
    14041 ccccagtctt tttgcgacat cgaccaccaa tttatcatga gaagacacga ctttaacatt
    14101 tcccaaatgt ttcacagcct tttctaaccc ccgtttacga ttttcaagag ggagaaatcc
    14161 ttgtttgtgg ggattaaaaa aaatacccac ataaagctta tcaaaaagtc tgctcgcccg
    14221 ttcaatgata tccagatgcc catttgtcat cggatcaaat gagcctgtga ataagccaat
    14281 cttatctgac atagactgtc accttactaa ttccataaat cttttccttc cagataccca
    14341 gacaggcaat ttcttctgga agttcaacgg ctttatccgt ctcgcacaca accataacat
    14401 cttcagaaaa aagctctctc tcagccattt tttcaatatc tgctacgatt tgttccttgg
    14461 catagggagg gtctaagaaa acgaggtcaa attccccaga tacctgttcc aatgcccttt
    14521 ctgcatccat cttgaggagt tgaaattttc caacttcctt ggtcatctgg atattttcag
    14581 ccacgatggt ctgagcctta cggtctcgct ccaccaaaac agcactggac atgccacgcg
    14641 atactgcttc gatagataaa ccaccactac ctgcataaag gtccaagact cgtcccactt
    14701 caaagtaggg accaatcatg ttaaaaatgg ctcccctaac cttatccgaa gtaggtcttg
    14761 tcgtcttgcc ttctagtgtc ttgaggggac gtcccccata gattcctgat acgattttca
    14821 tactgtttat tataccaaat tatagacaaa aagagaaaga aaaccgaacc ttgcggttcg
    14881 attctctaca aaatattttc gtaagtatcg cggacttctt gaggccaaac acttgtttgc
    14941 acttctccga tgtgtctctt gcgaagtagg aacatggcca tacgagattg tccaattcct
    15001 ccaccgattg tcaatgggaa tagaccattc aacaaagact tgtgccattc caattccaag
    15061 cggtcttcat cacctgtaat ttccacctga cgtctaagag tttcttcatc tacacgaatt
    15121 cccatagaag acaactcaaa ggctccacct aaagactcat tccagacaag aatatcacca
    15181 tttagaccct tgtagccatt ctcagactcg cttgtccagt catcatagtc tggtgcacgt
    15241 ccatcgtgcg gtttaccatc tggcaactcg ccaccgatac caatcaaaaa gacggctcca
    15301 aattctttac aaatcgcatt ttcacgttct ttcggtgtca agtctgggta gcgttctacc
    15361 aattcttctg tatggataaa ggtaatttgt tttggcaaga tagactcgat gtcatagcgg
    15421 gcttcaacag ctagctcagt caggcgaata gccttataaa tcttctcaac tgtttctttt
    15481 agataaacga tgttacgctt accatttggg ataaccttct cccagtccca ctggtcaaca
    15541 taaacagagt gggttgcatc caaggaatcc tcatctggac gaagggcttt catgtggaca
    15601 aagagaccct ctccttcacc aaagccaaaa cgagccaagg tgtggcgttt ccatttagca
    15661 agtgagtgca ccacttcata agtagcatca gggatttgga gaaccttgac cgatactgga
    15721 ttttccacac cagacaggtt gtcctgcatt ccgtcaccga ccttactcaa gataggacct
    15781 tgaacttcga caacttctag cttatctttc aaatactggg taaaagtgtt tttgacaaag
    15841 gaaatttctt cttgttgatg gataaaactt ttcttcatag acaactcctc aaaaatttaa
    15901 taaacgtatt atacacctat ttccaagaaa agaaaagaga aaatttaaaa aaaatcagtg
    15961 tcactcaaaa cactgatttc atagaccttt tagtcattgc gaccaaagat acgtagaata
    16021 cttaggaaaa gattgataaa atcaagatag atgctgagag ccattgacac cacccagcct
    16081 gtcgctacac gaccttgtga ttgttcatag gcgaggcgaa tcttttggtt atcccaagca
    16141 atcaaccctg aaaaaaccaa aaccatggct acgctaatca tataatcaaa gaagccacta
    16201 gccaagaaaa tattgactac catagcaatc agtaaaccaa tgagagctgc catcattgct
    16261 cgaccaatcc cactcaagtc tttcttggta aagataccaa ctgccgccat gacaaagaag
    16321 agaagggcgc tcgatacaaa ggcagataaa actgtacccg gagtatagaa agccaccaca
    16381 aaactaaggg taaagccgtt taaaacggag taaagtaaaa atactggaag agcagctgga
    16441 ctatttcttg aagccatgct actggcaacg aaaaccaaag ccaactccgc aaaagtagca
    16501 atggtcaacc agagacgccc ctgcatcaac aagtaaacca actgagactg aaagaccgtc
    16561 aacataaggc ctgataccaa agcagatagt ccgattccca gaccaacaaa ggcataaacc
    16621 ttagcgtaaa attgattgag acctgcacgg tcatgtataa tagtgtgatt catccatttc
    16681 tccttttctg tgaacatctt tccttgatta taacaaaaaa agttaaaatt agcttaaatg
    16741 gaagattaaa ataccggctg caaccgcaac attcaaactc tcggcctgcc ccttcatgct
    16801 aatatggacc aactggtctg cactttcagc cataagggga ctaattcctt gaccctcatt
    16861 tcccataact agtacaaaat tttctataga aggcagttct ctgtaatcaa cagaatcttt
    16921 agatagggtt gttgctaaca ctgggatagc taccttttta gtttcgtcaa gaagcgcttg
    16981 actagtcatc cggtaaatgg gcagatggaa atgactacct tgcatggaac gtagggtttt
    17041 gagactgtag atgtctgccg acttatctga aacaatcact cctgtaaaac ctgctgcatc
    17101 cgcagttcga atgatagttc ctacattacc aggatcttgc acatcttcca aaaacaagaa
    17161 cttgccctga ctcaaatcag cttgtcctac ttcttctttt tgaaccacgg ccacaattcc
    17221 ctgtggagtc tgagaatccg ccaaatctag caaaatatcc tctgacaccc agacagtttg
    17281 cgaaaaagtt gctaactgat ctcgataatt ttctagggta aagattttct caatcgtcac
    17341 tccagcttga acagcttctt caaacaagtg ccagccttca atcaaatagg ctgacttgcg
    17401 gtattttttt tggtgtaatt tcttggtatt ttttaccaca gaattggctt ttgaggttat
    17461 aatagtcata gaaatattat aacacaatca aaggggtttg gtatgcaaaa ggttagaatg
    17521 attgcccaag gtagggtgca gggagtcggc tttcgttggg gtgtttatag cctggccctt
    17581 gaaatcggtg gcatcacagg tcgggtatgg aataacgacg atggcacagt ggaaatctta
    17641 gcccaagcag actcatcagc tattatggca aaatttatcc aagaaatccg aaaaggaccg
    17701 acaccttttt caaaagtcag ttacttagat gtcaaactga gcaactttcc tccctaccct
    17761 gactttaaaa tcgcaaatta ggtctccaga actattgtat attttgtaaa aaaacagtag
    17821 aatagaaagg tataattttt aaagaaggaa taaaaacagt gaaatctatt aaacgttttg
    17881 cactctcggc tatgggagtg gctatgttgc ttgtcttgac tggctgtgtc aatgtcgata
    17941 aaaccacagg tcagccaaca ggatttattt ggaatacgat cggagcgcct atggctgaag
    18001 ccatcaagta cttcgctact gataaaggtc taggctttgg tgtcgctatc attatcgtaa
    18061 ccattatcgt acgcttgatt atcttaccac ttggtatcta ccaatcatgg aaggcaacgc
    18121 ttcactctga aaagatgaac gccctcaagc acgtccttga gccacaccaa acgcgtctca
    18181 aagaagcgac tactcaagaa gaaaaactcg aagcccaaca agctctcttt gctgctcaaa
    18241 aagagcacgg tatcagcatg tttggcggtg taggatgttt ccctatcctc cttcaaatgc
    18301 ctttcttctc tgctatctac tttgctgccc aacatactga aggggttgct caagcaagct
    18361 acctaggcat tcctctaggt tctccaagta tgattttggt tgcctgtgct ggtgtccttt
    18421 actatcttca atcgctcctt tcacttcacg gagtagaaga tgaaatgcaa agagaacaaa
    18481 tcaagaaaat gatttacatg agcccactca tgatcgtcgt cttctccctc ttctcaccag
    18541 ctagtgtcac actttactgg gttgtcggtg gtttcatgat gattctccaa cagtttatcg
    18601 tcaactatat cgttcgtcca aaacttcgca aaaaagtccg tgaagaacta gccaagaacc
    18661 caccaaaagc aagtgctttc tctaaaccaa gtggacgaaa agacgttacc cctgaacaac
    18721 caactgctat cacaagcaag aaaaaacaca aaaatcgcaa cgctggaaaa caacgttcga
    18781 gataagaaga aaaaggctta gctgatttgc taagtctttt tgcaagtcaa aagcccgatg
    18841 tttccatcag gcttcttttc tatccatgta cacgtttcat ataatcttga taactttcgg
    18901 tatccatgag ttgtttagcg ttcttgacgc gatctgctgt tggtggtttg actccttcga
    18961 gggaatatgg aattccaagt tcacgccact tgaactcacc catggtgtga taaggtagaa
    19021 tttcaaactt atcaacattt ttgagggtct tgacgaactt accaagttca atcaagtcat
    19081 catctctgtc tgtcaatcct ggaactagca cgtggcgaat ccagacaggt tttccaatat
    19141 ctgatagata ctgggcacaa gccaagatat ttttattggt ttggctagtg acaatcttgt
    19201 gctgttcttc gttgatttcc ttgatatcca aaagaaccaa gtcagtgaca gccatgagtt
    19261 tgtcaaactt ctcaaggtaa cgtggtttat tacggaaagg aagagcacag gtgtccaagg
    19321 tacagtggat tccttgttcc ttagccttgg tgaagagagc aatcaggaaa tcaatctgca
    19381 agagagcttc tcctccactg actgtaatcc cacccttatt tccccagaaa ccacggtagc
    19441 gcaaggcctc tgtcaagaca tcatctaccg tccgttcacg tgacttattg gactccatag
    19501 cccaagtgtc tgggttgtgg caatactggc aacgcatgtg acagccctgc aaaaagacaa
    19561 taaagcgaat accaggccca tctactgacc caaagctttc tgtcgaatgc accattcctg
    19621 tcacttgtcc ataatcaatt gtttcttcag acatatcgtt accttccttg aaaacgtttt
    19681 atagttttat tatatcacga cttgaaggaa aaacaagatt tttgcctatt ttttagaaag
    19741 caatctgttt ttcaatttca ttttcaatct attatcagat tattcttcaa aatcgaagcc
    19801 atataaggtt gcaaaatagt cctcagggcg ctctgcacgg cggatttgac gggctttacc
    19861 ttcttcggta tagaggattt ccgcagaacg taatttggca ttatactggt agcccattga
    19921 aaatccgtgg gcacctgtat catgaatgac cagcaaatca ccgatttctg tatgaggcag
    19981 ttcgcgatta actgcaaatt tatcattgtt ttcacagagt gaaccgacca catctaccac
    20041 ttcagctggt ccatctggat gggtcacgtt gctaatatga tggtaagctc cgtacatagc
    20101 tggacgcatg aggttgactg ctgaggcatc cacacctaga taggtacggt aggtttcctt
    20161 cttatgagtg actcttgtga ctagagcacc gtgaggtgcc agcataaaac gacccaattc
    20221 ggtgaaaatc ttgacctgac caagacctgc tgacgtaaga acctcttcat acaccttacg
    20281 aactccctca ccaatcaagg cgatatcgtt cggctcctgg tctggatgat aattaacacc
    20341 aataccgcca gaaagattga taaagtctag cgaaatgccc aacttttcct tgatttcaac
    20401 agccagttca aagagctgac gagccaactc tggataatag agatgggtca cggtattgga
    20461 cgctaggaag gagtgaatcc caaaagtctt ggctcctttt tccttcaaga tagcaaaggc
    20521 ttcaaagagc tggtccttgg tcatgccaaa cttagcctcc ccaggattgt ccataatgtc
    20581 tgtccccagt tcaaaaacgc ctccaggatt ataacgacaa gagatgattt ctggaatgcc
    20641 tgctactctc tccagatgtt caatatcttc aaaggcatcc aagttaatgg tcgcacccaa
    20701 ttcacgtgca taggcgtatt ccttgtctgg cgtgttgttg gaagagaaca taatctcaga
    20761 acccagaaag tccagtttat ggctcatcaa aagctctaca taactagagc agtccacacc
    20821 acaaccttct tcttggagaa ttttcaaaat agctggagtt ggagtagcct taactgcaaa
    20881 atattcctta aagcccttgt tccacgaaaa agcttggttg acggctcttg ccttctcacg
    20941 aatccccttc tcatcataca agtgaaaggg agtcgggaac tcggcaacaa tcgcttctaa
    21001 ctcttctcta ttgataaatg gtgttttcat aagcttcctt ctattctgtt tgcaaagaaa
    21061 ggctgggaca atcgttccaa cctttagttc tatctcttat ttatcttcgt caaagatatt
    21121 gccaacctca acatcgatgg cttggttctt gacatcaata ttggttacct tcaagagtga
    21181 cttgtagtca aactgctttt cacgttcttc ttgggcattg tccgcaaaga ccgctacacc
    21241 tgccagttct gagtcgaact cgcgcaagag actaatcata ccattgaccg ttccgccacc
    21301 tttcaagaag tcatccacaa tcaagacacg gctgcctgcc ttaagactac gttttgaaag
    21361 gaacattttc tcgatacggt caccacttga acctgaaaca tagttgacgc taacagttga
    21421 accttcggta attttcaggt cacggcgcac aatgacaaaa gagacattga ggacattggc
    21481 aactgcattt gcaagtggca cacccttagt tgctacggtc ataaccgcgt caattttttg
    21541 gtccataaag cttttggcaa taatacgacc aatatttttc aagatggctg gtgtgctaag
    21601 caaatcagac agatagatat aaccacctgg caagatacgg tcactttctg acaacttggt
    21661 acgcaagtct tcaaccattt ccttagcatc ctgactcgaa atagacggtg tgaagatgac
    21721 acctccgcca gccccagtca ctgtctggat atgaccgatt tcaatttcct caaaggcgcg
    21781 tttgataatg acgatatctt ctgagatgga tgatttagca gactcatact tttcagcaaa
    21841 agtattgaga ctagttagtt tataaggatt attaatcaaa tagttggaaa tgacaaccat
    21901 ccgatcactt cttcttaatt tcatttctat ttcctattcc atttttattc aaaaaatcaa
    21961 aaagcaaact agaaagctgg tcgctggtgg ttcaaaacac tgttttgaga ttgtcaatag
    22021 aactgacaaa ccctgtaata tacctgcata tatacatacg acaaggcgat actaccctag
    22081 tttgaagaga ttttcgaaga gtattcattt ttgtctttta cttattatac catattcaca
    22141 taaaaaaacg aacattctta tcctaaaaaa tgctcatttt tcttaaatta ttaatctaaa
    22201 tctggtttat agaaggaacg attatccata gcgaagattt tattggtcat ctctccttta
    22261 tccaccaaag caagagctgt tgacatcatc atcatgcttg catccagatt gtcaatcata
    22321 tggataatct ctgcttccat aatgcgtgga cggactgggc ttccatactc aagcaagccg
    22381 tggtgactga ggatgacatg acgaagcaaa acgacttctt ccttggtatc atcgatgccg
    22441 agttccataa ctgtcttggt aatttcgcta tcaatgagag cgatatgtcc aagaagatta
    22501 cctcgcactg tgtactctgt ctggtctggc cccgtcaact cgatgacctt agctaagtca
    22561 tgcaacataa tccccgcata gagcaggctc ttattgagct gaggataaac ttcgctaata
    22621 gcgtctgcca aacgcaccat ggtcgccgta tgataggcca agcccgtttc aaaggcatgg
    22681 tggttggtct tggcagctgg ataggagtag aattccttat catacttggt gtagagattt
    22741 cggacaatcc gttgccagac aggattttca attttgaaaa tcatttgcga catgtagtca
    22801 cgaatttcct tgacatcaac tggtgacttg accttgaaat cagctgggtc attgggttca
    22861 ccagcttgag gcaggcggag agtaatttga ttgacttgag gggtattgtt ataaacttct
    22921 cggcgtcctt tcatgtggac aaccttacct gcggtaaagg cctcaatgtt atgaggttgg
    22981 gcatcccaga gcttcccatc aatctcgcca ctatcatctt ggaaggtaaa ggctaggtag
    23041 tttttcccag ctcgagtttg cctcaggtca gctgatttga ttaggtaaaa gccttcaaat
    23101 aactcatctt ttttcatgtg actaatcttc atattcttcc tcattttctt gaaaatggag
    23161 tagatcaagc gcaggctcac cttctgacaa ctcaatgtga cggagcgtcc gctcgatagc
    23221 tatggtacga cggtttaata attcatcaat attgccagag gcatgttgga gatgtttttg
    23281 tgccttgacc agaataccac caaacttgcc aaactcggtc ttgacactgg caagagtctt
    23341 gctgatatgg tcggcactct tttggatatt aagggtcttg aaaccaactg atagggagtt
    23401 aagaagggct gatagggtac ttggtcctgc aacaataatc tgttcttccc gtctcaaatc
    23461 atcaaagaag accggattgc ggacgatttc tgagtagaga ccttctgtcg gaacaaacaa
    23521 aactccaaaa ttggtcgtcc gaggtggtgc tatgtacttg ttcctaatat ccctagcaaa
    23581 gcgcttgacg cttgctagga gtgacttacg acagcgttca atctcatcct tgtcacctgt
    23641 ctcataggct tcttccaagc ggtaataatc tgccagtgga aacttagagt caattggcag
    23701 atagacgtat tcttggtcgc cttgtccggg taacttgatg gcatactcca ctcgttcact
    23761 agagttttca accgttgcgt attctcgttc gtactgggca ggtgtcatga tgtcttcaat
    23821 aatttgcccc agttgcaatt ctcccagaat ccctcgcgtc ttggttccag agagaacctt
    23881 gttaagagct ccgacatcac gggcaactgt ctgcatttct ccaaggccac gattgacaga
    23941 ctccagttgt ttagaaactg tctcaaagga agcctgtaag cgtgtctgca aggtcttttc
    24001 tagtttttcc tcgaccgtct ggcgcatttg ttccaaacgt tgctcatttg attcctgcaa
    24061 ggcttggaga cgttggtctg tcttgtctct agtttggagg agattatctg tcatttcttg
    24121 acggacttgg gtcagacctt ggtgcaattc aatccgcact tcttgcaaac ggtcgctgac
    24181 aaccacttcc aaatcttttt ggtctaactg gctggcttgt ctggcttggt caaagcggta
    24241 atccaactgg tctgacaaat gatctgcctg atcctccaag ctcttactta agtgtttctc
    24301 ctgcctatcc tgcctttgcc aaatcagaaa gagaccagct agattggcaa ttaataatag
    24361 aataagtaaa ctttccatcc tacctcctgt ccttgctatg cagtacgacc acataaccat
    24421 ctgggcaagt taccgacact tccctatcta tatattcgtt agaagcgtac acttttttaa
    24481 agaaaaaatt ttcctctgtc aactcatact tggctccaag aatagtcagc tggctatccc
    24541 gaactggcat aaaggctaga tagtcgtagt ctgaacgagg ttctagctga ctgattcctt
    24601 ctggacaata agtaatcaag ttttgcccat cctcaatttc tatttgatgc atatagggtg
    24661 ccaacttagg attgctaggc agaaagacat tggccaacat atggtcaata cggccaccca
    24721 aggcaccgaa aatagtgacc tgagcctgag gattttgttc aaagatggtt aagagagcca
    24781 attccagatc tgtatcatcc ttttctggtc gtgcttggac aaaatactgg gcaccttttt
    24841 gaatcacctg tcgctcttct tccgtcacag aatcaaaatc tccgactgct agagcaagag
    24901 gtaagtcttc ttccaagacc cagagcgagc ctcgatccac cccaacaaaa gcatcaaaat
    24961 ctgtccgata atgaccgcgg tttccgcctg caaaaactgc aaccctagtc cagttgtttt
    25021 ctgagagttt gtactcgctc attgacttct cccttaaaga cataggaacc tgctacaaaa
    25081 acagtcgcac cggcttcttt ggcttgagca atagtttggt catcaatccc accatccact
    25141 tcgatttcaa agttcaaacc tttttcctca cgaagagcaa ccaactcacg gaccttatcc
    25201 atggtttctg gcagaaaggc ttgcccacca aaacctggat taaccgtcat gactaaaact
    25261 tggtcaacta gatgaaggac gtgcttgatg gcttcaactg atgtgccagg attgataacg
    25321 actgaaggct taactccgag tgaacgaatt ttttggaggg cgccatgaat atgaggcgtt
    25381 gcttctacat ggatactgat gatgtctgca cctgcacgcg caaaatcttc cagatgatgc
    25441 tcagggtttg acaccatcaa gtggcaatcg aaaaccatct tactatgagg acgaaggctc
    25501 tcgaccacac ctgcaccaaa actgatttgc ggtacaaaat gactgtccat gatatcgata
    25561 tgggcatatt ctgccccagt tgcttctaga cgtttgattt cacgttcaaa gttggcataa
    25621 tctgctgcca gaattgacgg agcaatcttg tattgagaca taggtttctc cttattttgg
    25681 aatttttttg ctgacttttt tataggtttc tctacgattt tcaatttcac taaggaattg
    25741 caggtaattg tcaaaacgga aggttgcaat aacaccctct tcaacagctg gtttgacggc
    25801 acaagacggc tcatgggtat gggtacaagt acggaactta caatctcggc taacagtagc
    25861 aatctctggg aaagcctgat tgaggtcttc agcccttgat acttcatagt ccaaggatga
    25921 aaatcctggt gtatctgcga ttttaccccc gttgagattg taaaaactaa cagctcgagt
    25981 ggtatggcga ccgcgaccta gactgtctga aatttctccc gtttcaagat tgaggtctgg
    26041 tgcgatttta ttgagaagag ttgacttccc aacacctgtc tgccccataa agaccgtaac
    26101 cttgcctgtt aacaaagaca ggagttcctc tttactggtc acaaagtcat agccgatgtc
    26161 accataggtc tgctggtaaa aatccagttc tcccctatct tccaacaaat ccattttgga
    26221 aatatagaca atgggatgga tgcccttgtg ctccaaaaga accaagaaac gatccagcaa
    26281 attgctgtta aaatcaggtt ccttgacgga catgattact acagcttgat cgatattgac
    26341 aataggcgga cgaaccagac tgtttttccg ttcgtgaatt ttgaggatat agccttctga
    26401 attttcctcg gcagagaaat ctacccagtc cccaacataa ggggtatggc cttttttacg
    26461 gaaattccca cgcgcgcgtg tttgataaac ctggccatca ctctccacat agtagaaacc
    26521 tgccaaggct ttaatgattt gtccctgcat cttagagtcc ttgcccttta agtgcgtctg
    26581 ctagaccggc aaattctgcc aagctgagag cttccccacg cacacttggt gacaagcctg
    26641 cctggtccaa agccttggtc agcttgtcct tgacctcttc agtcttacca aagtaacctg
    26701 tcaagttatt ccacaaggtc ttgcggcgat gggtaaaact agccttggaa accttaaaga
    26761 aaaagttctc atcttctact gctacggctg gctctggacg acgcaccatt ttcaagatgg
    26821 ctgaatccac atttggcgct ggcacaaaga ccgtacgagg cacgataaag gcaaccttgg
    26881 ctgtcatgta atactgcacg gcgatagaca agctaccgta agccttggtg ttaggctggg
    26941 ctgaaatgcg gtccgctact tctttctgca tcatgaccac aaactcacaa aaaggaatgc
    27001 cactctcaat caagtgcatg agaataggcg tcgtgatgta gtaaggcaaa ttagccacta
    27061 ccttgattgg caggtcagga tttttaaaat tctggatatg ttgcgccaaa tcaaccttga
    27121 gaatatcttc gttaactacg gtcacattat caaaatcacg cagggtatct gccaaaattg
    27181 gcaccaaacg gtggtcaatc tcaaaagcca tgacttgggc tgcacgctca gccaaaaatt
    27241 ctgtcaaggc accaatacct ggcccgattt cgatgacatt gacctgatca tcaatttcag
    27301 ccgtatccac aattttttga aggatattgg tatccgtcaa aaaattttgc ccaaaggact
    27361 ttttaaaggt aaaaccgtga cgctccagca ctgccttggt cacgctataa tctgcaattc
    27421 tcatctattc tcttttctat tatctgttac tactattgta acacattccc cttgtatttt
    27481 gggaatgaaa atcataccaa aaaactatct ttcctcatga agaaagatag tcttggaaat
    27541 ctttaaaagt ggccttagca gttctctcga aacatgcgtt caaaaaaatc agcttcttga
    27601 taacttcgat tttcaggtag ggtcaaatcc acatcctcct ccacatcatc cactgtcagt
    27661 tcaaccgtct gaggtaaatt tttcaaacga taatgctttt gcctttgccc ttcaatacta
    27721 gcaaaatcaa gcaaatagcc atgcactcgg atttgaacca aaaggagatg attggtcata
    27781 ttttccatga tgaggatatc ttctaaacca cctctggcac taggataatc agataccctg
    27841 cgaatacgga ttttttcaca catgagcaag tttcctcata tcaatgattt catcagcctt
    27901 attccagact aggggattat gggtcgcaat gatgataatt cgattttcat ctttcaaatc
    27961 caccaagaga ttcataacct cctctgaatt ttcaggatca agagctgctg ttggttcatc
    28021 tgccaaaatc aagggtggat ttttcaaaat agtcttagca agggcaactc gttgggcctc
    28081 tcccccagat aaagtataga ttttttgttc taaatccaaa taccctagat taactttttc
    28141 taaagccccc acttgccttt ccaaacgttc tacttttgag attttctgac caacaaaacc
    28201 caaatccaaa ttttctttga ttgattggtt ttctaagagg ccgaaatttt gaaagagata
    28261 gcccatctgg tctcgaaaat actcacgagt gggaatggtt tttaaatctt tcccctgata
    28321 gagaaccctt ccaccatcta tcttttctag ctttcccaag atattcagca gcgtcgtctt
    28381 tccgcttcca ctctttccga ttaaggcata aaccttgccc ttctccagct tgagattcaa
    28441 accagagaaa atcgcgcggt cattaaattt cttttccaat ccttgaatat caatcatctt
    28501 attttccttt caatactgcc atgctaccag cttcttcttt tttgtcctgc cttactaaaa
    28561 tcaagagggc gttaagcgta aacaaagcta caactagagc gctaatcaaa ccatctcttg
    28621 tcaaaatact agataggact aggccaagca agagaactcc tccttgcgcc agtaaatact
    28681 tgccatgaag ctcatagatt gtcataccag caagacgttt aatcataagt tcccgtctga
    28741 actgctcaaa atagagaaga ttcatggaat caaataacaa gatagccgta gacagggtca
    28801 aaatcgtccc aataaggaga gagtaaaact ccacccgttt gtcatttagt accttggcaa
    28861 aaaatagctg gctttttacc aagtaagaaa ccttgtgata cagacctttc tcttttagag
    28921 ctgtgatgct gtctccatat cgatctagtt tcaaggcact attagccgta gttccccaca
    28981 acatccctgc aacaggtctt gttccagtcg cttgcggcgt tagaactaca ataatcggat
    29041 cttttaggta ctggcgtgtt gtcttgtacc catcattata gaggaaacgt tcctgtcctg
    29101 tttctgtaaa agctagcctt acctgtggga ggtagtgttt ctgactcgtc acttctacac
    29161 tttcagatga aaagttttgc aggtaatctg taaacaatcc ttggtagtag acagactgct
    29221 ctcgcaagct ctcaggcaag atcagcccaa actctccctc agacaagttt tgcatcttgt
    29281 ccataaactc tgaagaaacg gtaatctttt cttctatcag atagcgcggt gagacataga
    29341 taacattccc tgacggtgtg tagtcactga gacgattgcc atctaggtcc acttctgctc
    29401 catctgagaa attgtcaaca ttgctcataa tataaaaaga gtctgtattg gctaaccgtt
    29461 cttcagtaaa tgtctgccac tcacgattat ccttacgcgt tccttcttca tcggcaaatg
    29521 cactagacca accaaaggat agacggtaac ggtctgagga ctggctccat ttattgctag
    29581 ctctctccat ttcctgcatt tcacggtagt ggggtaggag agctgtcgca ctcgatccga
    29641 ccaccaatac agctaagagt tgccccacca tcatcaatgt catcatacgt ttgagaggga
    29701 gtttcccttt caatagatcc accagactat tttcctgtaa accaagtaga tagacgacac
    29761 ttagtaaggt agaaatccct gccaaggtca atccataaag tagaagagca atgatgacca
    29821 gttgcaccgt tgccataaac aaggcacctt gataccagag aatccccaat cccaaaagac
    29881 tggataccag cactgagcag ataagctgtc tcacatcttc taacactggt ctgagagcaa
    29941 ctccaaacaa gctctcacca gctattaagc gaatccctgc ctgacgaagg gatttgatcc
    30001 gataaatcag ggtcagactc ataaaggtca gcagaaaaat agctaaactc agtagcacca
    30061 taggggtggc cgtcaatagt aagactatcg aaaatggatc ttctccattc gaaacaaagc
    30121 cttgataacc aagctctttc aaggtggtct gtaagctcac tccatccaaa cttcctgata
    30181 caatcaagta agaccctaat aaatcactcg tttctgcact ctccttggag gaaatggtca
    30241 gaccttctgg aagctttccc tcaccataag tggcataggt aaagcgtgtt tctccagctt
    30301 catttggctc aacgattcga cgagcaatga gactattgtg ctctaccgcc aagtgactca
    30361 aagcctctgt cacctcttcg cgactagcta ctggttgatt tatcatcatt cctgtcacat
    30421 caacagaagg atagttacca aataaaatct cttctcgatg gtcaacggaa gttatcacca
    30481 tataaagcga tactaatacc attgaaatca aaataaataa acgtttcatc atttcctcca
    30541 aaaagttact tggtcacttc gtgagaaggt gaccacagtc actgcgatgt taagaagctt
    30601 attaagttct ttctgctagc aacatcttca aatacattat atccgttttt tcaagaacta
    30661 tcaaggacat caatgtcctt aaaatctaac catttcgtgt catttttagc ttatatctaa
    30721 aaaaacatcc ctaaagacga actttagaac tgttttgata cgaggacaag gacatctttg
    30781 tcactgattc actatgttta taaatatttt ttgacatcct cttgccattc ctctgtcaat
    30841 ttctttacta gaaattcatc tccaattagc tgcgcaaatg ttttagaaga ctgataaaat
    30901 gattcagcta aactaaaatc tctttcttct cttaatgctt gcttccacaa aaacataaat
    30961 acaagaggtt tcttttgata atcttgagtt ttatccataa tttcttgtaa acacgataga
    31021 taatcattaa aacaatcagt tactcctacc ttttcaagac aacacattcc tgaaataaga
    31081 gtatctctaa ttataaagta attctctatt tgagttacct tcttctgttc caaaagaaag
    31141 agcattagct tttcgatttg atttataaat ttggcaagat cttttctaat gagcatttga
    31201 taaaaaaaca aacgaatcaa caacatatca tttattgaat aagcttcttt ttctataatc
    31261 tgaggcatat gttcctcaag tattaactct gcatattcag gccttctagt tctagaagta
    31321 tccaaacacg cctgcaatac ttcacatgct aattgttctt ctttaggaag attatcaaag
    31381 tatgttttat aaatctcttc caaacacgct tccttcttat cgtactcctc ttctttacca
    31441 tagattggtt cacgtaaaat ctggtatttc aattctaaat aagcagaagg aagagctgaa
    31501 aaatccggca taaggctata aactggaact cctagccggc gagcaatata gtctaacttg
    31561 ggcaaactcg gttgcgaagc tccactttca attcgcgaca gttgacgaac tgtcagttct
    31621 tgctcatctc cacaaaaatc tggacgacta attccttttt ctatccgaat ctctttaatt
    31681 ttttgaccaa ttaacatcag acgcctcctc ttctacctgc ttcgaaaaat actgtgaaac
    31741 ttaaaaaatt gtaacggcta catctttcct actcataaga tttcatagct tcttccactt
    31801 ctgccaaagt aaccccaaac aactctaggc gtttgaggag ttgcttgccg ttggaatagc
    31861 cgattcggag agtctctccg agatattctc tacgcttacg gctgtctgcc cctgctagaa
    31921 aaccaaggcg aatcaaatcg ctacgactaa tgtcaaactg actctcatgt tcaaattgtt
    31981 ctgtcacttg agctagagcc gttttcaggt cttcatagct ggcatgctca attcccagag
    32041 aacgcccctt ggtcttggac ttgggaacag cttcatctcg cttgagaaag gcatgctgaa
    32101 ctgttggaat gaccatcatg atcatgcgcc gaatccgttc cccattaaaa tctgggtctg
    32161 taaagacaat gactccatga cgttggtgca ggcgctgaat ccgctctata tcctgagcat
    32221 tgatggcaga acctcgagtc tcataggtct ccacatcgaa ataacgtttg agattgaccg
    32281 tatcatcacg cccttcaacc acgataactt gagaaatttt ctctttcatt acttgctgtc
    32341 cagtccaaaa attcgttctg catttgcagt cgttgctacc gccagctctt ctgtcgtcat
    32401 accacgcaag tcagcgataa agtcgaccac atagcgagta taggctgttt tattttcacg
    32461 accacgcttg ggtacaggtg ctaagtaagg cgcatctgtt tccaccaaca tcttgtccaa
    32521 aggtaactct ttagctgctt cttggaggtc agttgccttc ttaaaagtca ccactcctga
    32581 gaaggaaatg gtcataccaa gatccacaaa cttctctgcc cactcaagcg tccctgaaaa
    32641 tgaatgcatg ataccaccac gaggaccaac gccctcactc ttgataatct cataggtatc
    32701 ttccagcgca tcacgggtat ggacaacaaa aggcaaatcc aagtccttag atagctgaat
    32761 ctgacggcga aaaacctgct cctgcacctc tttgggcgct gtcatccaat ggtaatctaa
    32821 gccaatttca cctaaagcca caaccttgga atgttttaac ttatccaaca agtaagcctc
    32881 aacttcctct gtataagtac cagcttctgt aggatgccaa ccaatagtcg catagagctg
    32941 ctcatactca tctaccaact ccaaggcatg ctcaatcgtc ggtttatcaa aaccaacaat
    33001 attcatctgt gtcacaccca tctcagcagc caaggcaatt tcttctgcct cacgacctgc
    33061 aaattcttct acattcaagt gtgtatgtgt atcaaaaatc atctcttcta acctcgtttt
    33121 ctatcttcta ttataccaaa aaagaggagg ggcacctaat ttttcggttt cccctcctct
    33181 cttcaataga gagctattct gctatctttt ctatccgata ttgcccatct cctattccac
    33241 agttagagac agaagagatt ggctacattg atagaatatt cctattcagc taaagtatgt
    33301 gattatcttg ggttctcact tttctcaccc atcactagga ttgatgactt ctcaaaatcc
    33361 cccttataat ctaactttaa gtagagaata gaattttcta gctgttctat tttctacgtc
    33421 tagtctttct tcctagcaaa gcaaaaccac ttgttgccac cactagacca agactagcaa
    33481 gtgctgcact tgatgctgaa cctgtgttag gtaattgttt gccagcaggt gactggtagg
    33541 taacatcttg cattgttcct gcagttgctt gcggtgcact tgttgtctta ggagcagcat
    33601 taggagccac tggtctctta ggaactgtaa ccatggcaat ggcttgcact ccaatcgttt
    33661 ctgcttcctt cacatcttcc atagttttcg cattttcaat ctctttgaga gctgcttgtt
    33721 tttctgcttc cactcttgct aaaagttctg ctttttcttt atcagaaagc ggtgcgcctt
    33781 tgatttcatc ctgcttgtcc ttggctgctt tttcaatggc atttttagct gattcttttt
    33841 cagtagctaa ttgcttagaa gcttgtgatt gagctgagtt ttgtgaacct gtgtgagctg
    33901 atccacccgc ctggctacca ccgccttcat tcgcattagc atctgagctt ccagtattgc
    33961 ctcccttatc tccacctggt gtattttcta gcttatactc tggtgtagct tcttgagtta
    34021 cagattcacc tttcgcagat tgttgaactg tatcttttcc tagaatcgtc actactgaac
    34081 catctgggaa tgtgattgtt gctgtaccat ctccagctac attgattgtc gctccgtcta
    34141 atgctgaacc atttgcttgt aaaatagcaa ccttaacttt ttcttcatct gttaaatgag
    34201 ttatatcttt tacatctact ctttgagcag gtgctttaac agttgatttt tcagcagtca
    34261 gtttagtgct atcttgtgct tttggatctt gtgctaccaa ttgatttcct ggaattgtaa
    34321 tagctgaacc atctttaaag acgattgttg ctgtaccatc tccacctact tcaactttat
    34381 agtaaaataa aataataaca ggacaaatcg atcaggacag tcaaatcgat ttctaacaat
    34441 gttttagaaa cagaggtgta ctattctagt ttcaatctac tgtaacatcg cttccgaatc
    34501 catttgcatc tactgaactt ttttacaaaa ataaaaatca gtagactcga gttcctactg
    34561 atttttgata gaattcgttt ggattatgca gccaaaactt tgcgtccttt acgacgacga
    34621 gctgccaata cgcgacgacc atttttagtt gacatacggt tacggaatcc gtgtttgcgc
    34681 gcacgacgaa gtttacttgg ttgataagta cgtttcacga tgaatacctc ctcatagatt
    34741 ttgtattcgt ttagccggct ataaagtgat cagttactaa acatacttta ctattctatc
    34801 tgattctttc acttttgtca atagctctag gcaaatgttt ccagcttttt cgaatgcaag
    34861 ccctatctac gatactgttt caagaaacga gtcatctttt cttggatttg ggctaactca
    34921 tcaacacgag gaaggtaaac gatacggaag tggtctggtt cctgccagtt aaagcctcga
    34981 ccatgaacca agagaacctt ttcctgcttc aagaaatcaa ggacaaactg ctcatcatca
    35041 tcgatacggt acatattgcg gtcgattttt gggaagatat agagccccgc cttgggttta
    35101 acggcagaca aacctggaat atcttgaatg gcattataga tgaaatttct ttgctcgtag
    35161 attcgtccac caggaagaag caattcatcg actgattggt gaccccccaa ggaagtttgt
    35221 acgacttgtt gggccaaaac gttagagcaa aggcgcatat tggacagcat attgagccct
    35281 tcgatatagc ccttaacatg agtcttaggg ccagacaaga ccatccaacc cacacggaaa
    35341 cctgctatgc ggtgggattt tgacagacca ttcatgctga cacagaagac atctggtgcc
    35401 aagctcgcca caggcgtatg cacatgtccg tccattacca tgcggtcata gatttcatcc
    35461 gcaaagataa tcaaatcgtt ttgacgggca atctcaataa tctccagcaa gagttcctta
    35521 ggataaaggg ctccagttgg gttatttgga ttgataagga caattgcctt ggtattggaa
    35581 gtgatttttg acttgatatc gtcaatatct gggtaccatt ccacagcttc atcacagata
    35641 tagtgaacgg catttcctcc agctaggctg acagccgccg tccatagagg atagtctggc
    35701 attggcacta agacctcatc cccattgtcc aaaagcccct gcatggacat gacaatcaac
    35761 tcactgacac catttccaag gtaaatatca tcaatatcta cgtttgggaa tttcttcagc
    35821 tggcaatact gcatgatggc cttacgggct gagaaaatcc ctttggagtc agaatagcct
    35881 tcactatcac gcgcattcat aatcaagtca tgaatgacct cgtctggcgc tgtaaagcca
    35941 aattctgctg gatttcctgt attcagacgt aaaatctttt ctccattcgc tcgcatccgc
    36001 atggcttctt ccaatacagg accacggata tcataagcaa catgctctaa cttactagac
    36061 ttgttatatt ctttcatctt gtttcctcaa ttcatcagga tagtaacatt ataccactag
    36121 aaagagaatg cgacaagttt actgaaatgt gttactagtt tcgtatagaa gaaaaacctt
    36181 gctaaaggcg aggttaagaa cgtagagata gcaggtattt tgcaaagaca catcacatac
    36241 caaaagataa acctagaaaa aagcaagcct agaaaggagt cataaagaag attaaacatg
    36301 aggaaaagcc cccatatcat caagtaatcc caacgcccac ttcgttttgc aactagaaat
    36361 aagattagat ggtagagtac atgaaaaact aaaaatccaa taaaacttgg ataaagaagc
    36421 ggtaccagac tctctaattg acagatcatg atcacttcta ccaagactga aactaggata
    36481 attccataat aaaggaaatt tgatttttga aaagagtttt tcatcattct cccttccttc
    36541 acttctttcc tgctatcctt tcttttattt tatcatatat ttatatagag tttaagccct
    36601 aatattcttt tgaaaaatac tttttttgct ttacttctat agtgaaaagg tttacaatta
    36661 tagtaagaaa atttcaggag gtttcattat ggcacaacgt taccaaaata tcatggtcgc
    36721 aatcgatggt tctaaggaag cggacttggc ttttgtcaag ggagttcatt ctgctctacg
    36781 aaacgacgct aaactcacca tcgcacatgt cattgacaca cgcgctctcc aaagcgtatc
    36841 cacctttgat gctgaagttt acgaagaact ccaagtcgac gctgaaagtc tgatgaaaga
    36901 gtacgaaaaa cgtgctaaag atgctggggt agcagatgtt catatcgtca ttgaaatggg
    36961 aaatccaaag accctgctag cacgtactat tccagatgcc gaggaagtgg acctcatcct
    37021 cgttggcgca actggtctca acgcctttga acgcctcttg gtcggctctt catctgaata
    37081 catactccgc catgctaagg tcgatttgct ggttgtgaga gaacaagaaa aaaccttata
    37141 atcacaaaga aaaggagccc ctagctcctt tttgtttacg atttatttct ctctttatgg
    37201 cgttcgtaag ccttgagctg gcgctgcagt tcctttttaa tagcaggttc tggagcatat
    37261 ttttcttccc aattatctgg ttttaagatt ttatgggtca ctggatcaaa atgagccttg
    37321 ccatctggaa aaattttccc catattggcc tgatggacaa tatcaaaaat acgttctggg
    37381 tccaccccca tcaagacaaa actgccgtag gtgaagtaaa gcgtgtcaat caaggcatcc
    37441 acttgcccta tcaaatcttg ctgagcaggt gtcttcttgg ctactttatc tgctgcctta
    37501 tcaagggcct gatgaagttg cgatacagct tgaccaaaat cttcttcaga aggactggct
    37561 gctcgaacaa actccaccaa ttcttctatt ttaaaaccag ccctatgggt tgcaccctct
    37621 aaatcccaag ctcgaggttc ttcttgggtt cgttcatcca tcatgtggtg gaaggtcttg
    37681 accttattga aatgatagtc acggctgaca aagacttttt ctgaagacaa aacaccaaaa
    37741 tgttccaagg ccttgtggat tccatcttgt tgattgctag tggtaatgtg cttggcagct
    37801 tccttgacac tgctactacc atttcccata gcgaccgaca taccaacgcc agccagcatt
    37861 tcaagatcat tatctgagtc accaaaagcc atgacttggt tgaggtcaaa gccatattct
    37921 ttcccaactc ggcgaatgcc ttctaattta gaatttccct gattgatgac atccgatgca
    37981 aaaggattgc tacgtgtcaa tttcaagtct tcaaaatcag ctgccgcctt ctcagattct
    38041 tctggtgtca tcagcatcaa aacttggtag ataggctgat tcatcaggtg aagcaggtcc
    38101 tcttcctttt ggggaacaac cttgctgacc atgcgattaa aagactgact caccgtccga
    38161 gttaaaacag agggaacgaa gcgactaatt cgttgggaaa aagaacccag accaaaggac
    38221 atgattttag aacccaacat ggcatccttg gtccctagag caatctccgt gccctctttt
    38281 ttagcatagc taattagatg gcgcaaatgt aacttggaaa tagggctcgt gaacaagact
    38341 ctgtctttac taaagatata ctggccatta taggttaccg caaaatccag atccaaatcg
    38401 tccatcaatt ccttaacaaa aaaaggtcct cgccctgtcg ctacgccaac tagtacccct
    38461 tgttctttga caatcttaat cgcatcctta gtggatttca aaacactctt gcgattgttg
    38521 accaaggttc catcgatatc aaaaaaaaca gctttgactt ccatcctatc ccaatctccc
    38581 cttttgtgat acaatgatta taccacattt cagaaagagt gagtaaatca tgcctaagaa
    38641 aatccttgtt ttacatacgg gtggaactat ttccatgcag gccgatgctt ctggcgctgt
    38701 tgtgacgagt tcagataatc ccatgaacca tgtgtccaac ccacttgaag gaatccaagt
    38761 ccacgccttg gactttttta accttccaag tccccatatc aaacccaaac atatgctggt
    38821 cctctaccag aaaattaaag aggaagcaga taactacgat ggagtggtga tcacacacgg
    38881 aaccgatact ttagaggaaa cagcctattt ccttgatacc atggaagttc cccatatgcc
    38941 tatcgttcta acaggagcca tgcgtagctc caatgagctc ggtagtgatg gtgtttataa
    39001 ttacctaagt gctttacgag tggccagcga tgacagggct gctgacaaag gagttttggt
    39061 cgttatgaac gatgaaatcc acgctgccaa gtatgtcacc aaaacacata cgactaatgt
    39121 cagcaccttc cagactccaa cacatggccc ccttggtctc atcatgaaac aggaaatcct
    39181 ctacttcaaa acagctgaac ctcgtgttcg ctttgacctt gatcacatac aaggtttagt
    39241 ccctatcatc tcggcttatg ctggtatgac agatgagctg attgatatgc tggatttaga
    39301 acacttggac ggtttgatta tccaagcctt cggagctggt aatattccca aagaaacggc
    39361 tcaaaaatta gaaagccttc tgcaaaaagg aattccagtc gctctggtat cacgatgctt
    39421 taacggtatt gccgagcctg tttatgcata ccagggtggg ggcgtacagt tgcaaaaagc
    39481 aggcgttttc tttgttaaag aactcaacgc ccaaaaagct cgcttgaaac tcctcatcgc
    39541 cctcaatgcc ggactaacag gacaggcttt gaaagactat atggaaggct aatactcttc
    39601 gaaaatctct gcaaaccacg tcacgtcgcc ttaccgtatg tatggttact gacttcgtca
    39661 gttt
//
LOCUS       DBDESG010000013        39042 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.013, whole genome shotgun sequence.
ACCESSION   DBDESG010000013 DBDESG010000000
VERSION     DBDESG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.013"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            148..6849
                     /gene="bgaA"
                     /locus_tag="ACKB38_000661"
     CDS             148..6849
                     /gene="bgaA"
                     /locus_tag="ACKB38_000661"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGL7979473.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNIVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVNFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLQKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLID
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKDQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     VPKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEITVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVTQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPSTGSAASQAAVAAGLTLLGLSAGLVVTKGKKED"
     gene            complement(7097..7720)
                     /locus_tag="ACKB38_000662"
     CDS             complement(7097..7720)
                     /locus_tag="ACKB38_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7979474.1"
                     /translation="MEELVTLDCLFIDRTKIEANANKYSFVWKKTTEKFSAKLQEQIQ
                     VYFQEEITPLLIKYAMFDKKQKRGYKESAKNLANWHYNDKEDSYTHPDGWYYRFHHTK
                     YQKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQGRQIFAQRK
                     IDVEPVFGQIKASLGYKRCNLRGKRQVRIDMGLVLMANNLLKYSKMK"
     gene            complement(7708..7953)
                     /locus_tag="ACKB38_000663"
     CDS             complement(7708..7953)
                     /locus_tag="ACKB38_000663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7979475.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEERHFYTS
                     YHAFDRPSYHPKMLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            complement(8163..8522)
                     /locus_tag="ACKB38_000664"
     CDS             complement(8163..8522)
                     /locus_tag="ACKB38_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGL7979476.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8614..9570
                     /locus_tag="ACKB38_000665"
                     /pseudo
     CDS             8614..9570
                     /locus_tag="ACKB38_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9554..10111
                     /locus_tag="ACKB38_000666"
     CDS             9554..10111
                     /locus_tag="ACKB38_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGL7979477.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPAADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10104..10358
                     /locus_tag="ACKB38_000667"
     CDS             10104..10358
                     /locus_tag="ACKB38_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979478.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAMGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10370..12424
                     /locus_tag="ACKB38_000668"
     CDS             10370..12424
                     /locus_tag="ACKB38_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGL7979479.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12603..13463
                     /locus_tag="ACKB38_000669"
     CDS             12603..13463
                     /locus_tag="ACKB38_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGL7979480.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13674..14384
                     /gene="ccdA2"
                     /locus_tag="ACKB38_000670"
     CDS             13674..14384
                     /gene="ccdA2"
                     /locus_tag="ACKB38_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGL7979481.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            14404..14970
                     /locus_tag="ACKB38_000671"
     CDS             14404..14970
                     /locus_tag="ACKB38_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGL7979482.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14981..16093
                     /gene="msrB"
                     /locus_tag="ACKB38_000672"
     CDS             14981..16093
                     /gene="msrB"
                     /locus_tag="ACKB38_000672"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGL7979483.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16361..17098
                     /locus_tag="ACKB38_000673"
     CDS             16361..17098
                     /locus_tag="ACKB38_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGL7979484.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17095..18786
                     /locus_tag="ACKB38_000674"
     CDS             17095..18786
                     /locus_tag="ACKB38_000674"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGL7979485.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            18902..19465
                     /locus_tag="ACKB38_000675"
     CDS             18902..19465
                     /locus_tag="ACKB38_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGL7979486.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKF"
     gene            19487..25207
                     /locus_tag="ACKB38_000676"
     CDS             19487..25207
                     /locus_tag="ACKB38_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGL7979487.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPEVPE
                     EEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLET
                     AKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRSWQN
                     LQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKILERE
                     AVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYKEYT
                     LSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETNESLITTIP
                     DDKSNYYLKITSNNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEY
                     VHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTG
                     KLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSV
                     ITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRG
                     IVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEV
                     GAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNR
                     SFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQDYNAEYNQ
                     AYKNLEKLIPFYNKDYIVYQGNKLNKEHHLNTKEVLSVTAMNNNEFITNLDEANKIIV
                     HYADGTKDYFNLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVE
                     LQSQTMYQHLNRLGDYRVNAIKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAA
                     RQMIRDKVEKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIE
                     IGSKENNIKGSRTFDAFGQVLAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDH
                     VYIAERASEVEEIKNSKHRAFDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSA
                     ERLGKKSLEDIKDIVNKAADGYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGG
                     WVEKYGRYNTDKVYTPLREFFGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMV
                     GEYGISVYTHETTHVNDRAIYLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGI
                     NMVFKRKNDGNQWYITDPKTLKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLN
                     SAWFKKIDREYRDNNKLNQWDKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIY
                     KPEAISYNDQSPYVGVRMMTGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYA
                     SNKYKQQSKTDGESVLSDEYIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVN
                     GSSVSSYDDLLTLFKEAVKKDAETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTNSF
                     KTSIFK"
     gene            25268..26989
                     /gene="pabB"
                     /locus_tag="ACKB38_000677"
     CDS             25268..26989
                     /gene="pabB"
                     /locus_tag="ACKB38_000677"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGL7979488.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDIVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMTVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            26990..27607
                     /locus_tag="ACKB38_000678"
     CDS             26990..27607
                     /locus_tag="ACKB38_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGL7979489.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTYPYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            27657..28655
                     /locus_tag="ACKB38_000679"
     CDS             27657..28655
                     /locus_tag="ACKB38_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HGL7979490.1"
                     /translation="MNKRLFSKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEVQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            28782..29741
                     /locus_tag="ACKB38_000680"
     CDS             28782..29741
                     /locus_tag="ACKB38_000680"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGL7979491.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            29838..30677
                     /locus_tag="ACKB38_000681"
     CDS             29838..30677
                     /locus_tag="ACKB38_000681"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGL7979492.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(30725..30895)
                     /locus_tag="ACKB38_000682"
     CDS             complement(30725..30895)
                     /locus_tag="ACKB38_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGL7979493.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            31016..31900
                     /gene="miaA"
                     /locus_tag="ACKB38_000683"
     CDS             31016..31900
                     /gene="miaA"
                     /locus_tag="ACKB38_000683"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGL7979494.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            31893..33131
                     /gene="hflX"
                     /locus_tag="ACKB38_000684"
     CDS             31893..33131
                     /gene="hflX"
                     /locus_tag="ACKB38_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGL7979495.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            33124..33747
                     /locus_tag="ACKB38_000685"
     CDS             33124..33747
                     /locus_tag="ACKB38_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGL7979496.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            33762..34691
                     /gene="rnz"
                     /locus_tag="ACKB38_000686"
     CDS             33762..34691
                     /gene="rnz"
                     /locus_tag="ACKB38_000686"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGL7979497.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            34712..35467
                     /locus_tag="ACKB38_000687"
     CDS             34712..35467
                     /locus_tag="ACKB38_000687"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGL7979498.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(35521..36429)
                     /locus_tag="ACKB38_000688"
     CDS             complement(35521..36429)
                     /locus_tag="ACKB38_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGL7979499.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            36493..36762
                     /locus_tag="ACKB38_000689"
     CDS             36493..36762
                     /locus_tag="ACKB38_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGL7979500.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            36762..37142
                     /locus_tag="ACKB38_000690"
     CDS             36762..37142
                     /locus_tag="ACKB38_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGL7979501.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            37231..37458
                     /locus_tag="ACKB38_000691"
     CDS             37231..37458
                     /locus_tag="ACKB38_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979502.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(37498..38223)
                     /locus_tag="ACKB38_000692"
     CDS             complement(37498..38223)
                     /locus_tag="ACKB38_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HGL7979503.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGAVTANKDKELPTVMDLLPSNIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDDTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            38385..>39042
                     /locus_tag="ACKB38_000693"
     CDS             38385..>39042
                     /locus_tag="ACKB38_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGL7979504.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     Y"
BASE COUNT        12106 a         7200 c         8634 g        11102 t
ORIGIN      
        1 aatcatatct acggcaaggc gacactgacg aagtttgaat tggattttcg cagagtgtaa
       61 attgttgaaa ctaggttgtc ataccatgta taccacttgg gctttggtag tagctaactg
      121 cactaaatat aatataagga gggttttatg gggaaaggcc attggaatcg gaaaagagtt
      181 tatagcattc gtaagtttgc tgtgggagct tgctcagtaa tgattgggac ttgtgcagtt
      241 ttattaggag gaaatatagc tggagaatct gtagtttatg cggatgaaac acttattact
      301 catactgctg agaaacctaa agaggaaaaa atgatagtag aagaaaaggc tgataaagct
      361 ttggaaacta aaaatatagt tgaaaggaca gaacaaagtg aacctagttc aactgaggct
      421 attgcatctg agaagaaaga agatgaagcc gtaactccaa aagaggaaaa agtgtctgct
      481 aaaccggaag aaaaagctcc aaggatagaa tcacaagctt caaatcaaga aaaaccgctc
      541 aaggaagatg ctaaagctgt aacaaatgaa gaagtgaatc aaatgattga agacaggaaa
      601 gtgaatttta atcaaaattg gtactttaaa ctcaatgcaa attctaagga agccattaaa
      661 cctgatgcag acgtatctac gtggaaaaaa ttagatttac cgtatgactg gagtatcttt
      721 aacgatttcg atcatgaatc tcctgcacaa aatgaaggtg gacagctcaa cggtggggaa
      781 gcttggtatc gcaagacttt caaactagat gaaaaagacc tcaagaaaaa tgttcgcctt
      841 acttttgatg gcgtctacat ggattctcaa gtttatgtca atggtcagtt agtggggcat
      901 tatccaaatg gttataacca gttctcatat gatatcacca aataccttca aaaagatggt
      961 cgtgagaatg tgattgctgt ccatgcagtc aacaaacagc caagtagccg ttggtattca
     1021 ggaagtggta tctatcgtga tgtgacttta caagtgacag ataaggtgca tgttgagaaa
     1081 aatgggacaa ctattttaac accaaaactt gaagaacaac aacatggcaa ggttgaaact
     1141 catgtgacca gcaaaatcgt caatacggac gacaaagacc atgaacttgt agccgaatat
     1201 caaatcgttg aacgaggtgg tcatgctgta acaggcttag ttcgtacagc gagtcgtacc
     1261 ttaaaagcac atgaatcaac aagcctagat gcgattttag aagttgaaag accaaaactc
     1321 tggactgttt taaatgacaa acctgccttg tacgaattga ttacgcgtgt ttaccgtgac
     1381 ggtcaattgg ttgatgctaa gaaggatttg tttggttacc gttactatca ctggactcca
     1441 aatgaaggtt tctctttgaa tggtgaacgt attaaattcc atggagtatc cttgcaccac
     1501 gaccatgggg cgcttggagc agaagaaaac tataaagcag aatatcgccg tctcaaacaa
     1561 atgaaggaga tgggagttaa ctccatccgt acaacccaca accctgctag tgagcaaacc
     1621 ttgcaaatcg cagcagaact aggtttactc gttcaggaag aggcctttga tacgtggtat
     1681 ggtggcaaga aaccttatga ctatggacgt ttctttgaaa aagatgccac tcacccagaa
     1741 gctcgaaaag gtgaaaaatg gtctgatttt gacctacgta ccatggtcga aagaggcaaa
     1801 aacaaccctg ctatcttcat gtggtcaatt ggtaatgaaa taggtgaagc taatggtgat
     1861 gcccactctt tagcaactgt taaacgtttg gttaaggtta tcaaggatgt tgataagact
     1921 cgctatgtta ccatgggagc agataaattc cgtttcggta atggtagcgg agggcatgag
     1981 aaaattgctg atgaactcga tgctgttgga tttaactatt ctgaagataa ttacaaagcc
     2041 cttagagcta agcatccaaa atggttgatt tatggatcag aaacatcttc agctacccgt
     2101 acacgtggaa gttactatcg ccctgaacgt gaattgaaac atagcaatgg acctgagcgt
     2161 aattatgaac agtcagatta tggaaatgat cgtgtgggtt gggggaaaac agcaaccgct
     2221 tcatggactt ttgaccgtga caacgctggc tatgctggac agtttatctg gacaggtacg
     2281 gactatattg gtgaacctac accatggcac aaccaaaatc aaactcctgt taagagctct
     2341 tactttggta tcgtagatac agccggcatt ccaaaacatg acttctatct ctaccaaagc
     2401 caatgggttt ctgttaagaa gaaaccgatg gtacaccttc ttcctcactg gaactgggaa
     2461 aacaaagaat tagcatccaa agtagctgac tcagaaggta agattccagt tcgtgcttat
     2521 tcgaatgctt ctagtgtaga attgttcttg aatggaaaat ctcttggtct taagactttc
     2581 aataaaaaac aaaccagcga tgggcggact taccaagaag gtgcaaatgc taatgaactt
     2641 tatcttgaat ggaaagttgc ctatcaacca ggtaccttgg aagcaattgc tcgtgatgaa
     2701 tctggcaagg aaattgctcg agataagatt acgactgctg gtaagccagc ggcagttcgt
     2761 cttattaagg aagaccatgc gattgcagca gatggaaaag acttgactta catctactat
     2821 gaaattgttg acagccaggg gaatgtggtt ccaactgcta ataatctggt tcgcttccaa
     2881 ttgcatggcc aaggtcaact ggtcggtgta gataacggag aacaagccag ccgtgaacgc
     2941 tataaggcgc aagcagatgg ttcttggatt cgtaaagcat ttaatggtaa aggtgttgcc
     3001 attgtcaaat caactgaaca agcagggaaa ttcaccctga ctgcccactc tgatctcttg
     3061 aaatcgaacc aagtcactgt ctttactggt aagaaagaag gacaagagaa gactgttttg
     3121 gggacagaag tgccaaaagt acagaccatt attggagagg cacctgaaat gcctaccact
     3181 gttccgtttg tatacagtga tggtagccgt gcagaacgtc ctgtaacctg gtcttcagta
     3241 gatgtgagca agcctggtat tgtaacggtg aaaggtatgg ctgacggacg agaagtagaa
     3301 gctcgtgtag aagtgattgc tcttaaatca gagctaccag ttgtgaaacg tattgctcca
     3361 aatactgact tgaattctgt agacaaatct gtttcctatg ttttgattga tggaagtgtt
     3421 gaagagtatg aagtggacaa gtgggagatt gccgaagaag ataaagctaa gttagcaatt
     3481 ccaggttctc gtattcaagc gaccggttat ttagaaggtc aaccaattca tgcaaccctt
     3541 gtggtagaag aaggcaatcc tgcggcacct gcagtaccaa ctgtaacggt tggtggtgag
     3601 gcagtaacag gtcttactag tcaaaaacca atgcaatacc gcactcttgc ttatggagct
     3661 aagttgccag aagtcacagc aagtgctaaa aatgcagctg ttacagttct tcaagcaagc
     3721 gcagcaaacg gcatgcgtgc gagcatcttt attcagccta aagatggtgg ccctcttcaa
     3781 acctatgcaa ttcaattcct tgaagaagcg ccaaaaattg ctcacttgag cttgcaagtg
     3841 gaaaaagctg acagtctcaa agaagaccaa actgtcaaat tgtcggttcg agctcactat
     3901 caagatggaa cgcaagctgt attaccagct gataaagtaa ccttctctac aagtggtgaa
     3961 ggggaagtcg caattcgtaa aggaatgctt gagttgcata agccaggagc agtcactctg
     4021 aacgctgaat atgagggagc taaagaccaa gttgaactca ctatccaagc caatactgag
     4081 aagaagattg cgcaatccat ccgtcctgta aatgtagtga cagatttgca tcaggaacca
     4141 agtcttccag caacagtaac agttgagtat gacaaaggtt tccctaaaac tcataaagtc
     4201 acttggcaag ctattccgaa agaaaaacta gactcctatc aaacatttga agtactaggt
     4261 aaagttgaag gaattgacct tgaagcgcgt gcaaaagtct ctgtagaagg tatcgtttca
     4321 gttgaagaag tcagtgtgac aactccaatc gcagaagcac cacaattacc agaaagtgtt
     4381 cggacatatg attcaaatgg tcacgtttca tcagctaagg ttgcatggga tgcgattcgt
     4441 ccagagcaat acgctaagga aggtgtcttt acagttaatg gtcgcttaga aggtacgcaa
     4501 ttaacaacta aacttcatgt tcgcgtatct gctcaaactg agcaaggtgc aaacatttct
     4561 gaccaatgga ccggttcaga attgccactt gcctttgctt cagactcaaa tccaagcgac
     4621 ccagtttcaa atgttaatga caagctcatt tcctacaata accaaccagc caatcgttgg
     4681 acaaactgga atcgtactaa tccagaagct tcagtcggtg ttctgtttgg agattcaggt
     4741 atcttgagca aacgctccgt tgataatcta agtgtcggat tccatgaaga ccatggagtt
     4801 ggtgtaccga agtcttatgt gattgagtat tatgttggta agactgtccc aacagctcct
     4861 aaaaacccta gttttgttgg taatgaggac catgtcttta atgattctgc caactggaaa
     4921 ccagttacta atctaaaagc ccctgctcaa ctcaaggctg gagaaatgaa ccactttagc
     4981 tttgataaag ttgaaaccta tgctgttcgt attcgcatgg ttaaagcaga taacaagcgt
     5041 ggaacgtcta tcacagaggt acaaatcttt gcgaaacaag ttgcggcagc caagcaagga
     5101 caaacaagaa tccaagttga cggcaaagac ttagcaaact tcaaccctga tttgacagac
     5161 tactaccttg agtctgtaga tggaaaagtt ccggcagtca cagcaagtgt tagcaacaat
     5221 ggtctcgcta ccgtcgttcc aagcgttcgt gaaggtgagc cagttcgtgt catcgcgaaa
     5281 gctgaaaatg gcgacatctt aggagaatac cgtctgcact tcactaagga taagagctta
     5341 ctttctcata aaccagttgc tgcggttaaa caagctcgct tgctacaagt aggtcaagca
     5401 cttgaattgc cgactaaggt tccagtttac ttcacaggta aagacggcta cgaaacaaaa
     5461 gacctgacag ttgaatggga agaagttcca gcggaaaatc tgacaaaagc aggtcaattt
     5521 actgttcgag gccgtgtcct tggtagtaac cttgttgctg agatcactgt acgagtgaca
     5581 gacaaacttg gtgagactct ttcagataac cctaactatg atgaaaacag taaccaggcc
     5641 tttgcttcag caaccaatga tattgacaaa aactctcatg accgcgttga ctatctcaat
     5701 gacggagatc attcagaaaa tcgtcgttgg acaaactggt caccaacacc atcttctaat
     5761 ccagaagtat cagcgggtgt gattttccgt gaaaatggta agattgtaga acggactgtt
     5821 acacaaggaa aagttcagtt ctttgcagat agtggtacgg atgcaccatc taaactcgtt
     5881 ttagaacgct atgtcggtcc agagtttgaa gtgccaacct actattcaaa ctaccaagcc
     5941 tacgacgcag accatccatt caacaatcca gaaaattggg aagctgttcc ttatcgtgcg
     6001 gataaagaca ttgcagctgg tgatgaaatc aacgtaacat ttaaagctat caaagccaaa
     6061 gctatgagat ggcgtatgga gcgtaaagca gataagagcg gtgttgcgat gattgagatg
     6121 accttccttg caccaagtga attgcctcaa gaaagcactc aatcaaagat tcttgtagat
     6181 ggaaaagaac ttgctgattt cgctgaaaat cgtcaagact atcaaattac ctataaaggt
     6241 caacggccaa aagtctcagt tgaagaaaac aatcaagtag cttcaactgt ggtagatagt
     6301 ggagaagata gctttccagt acttgttcgc ctcgtttcag aaagtggaaa acaagtcaag
     6361 gaataccgta tccacttgac taaggaaaaa ccagtttctg agaagacagt tgctgctgta
     6421 caagaagatc ttccaaaaat cgaatttgtt gaaaaagatt tggcatacaa gacagttgag
     6481 aaaaaagatt caacactgta tctaggtgaa actcgtgtag aacaagaagg aaaagttgga
     6541 aaagaacgta tctttacagc gattaatcct gatggaagta aggaagaaaa actccgtgaa
     6601 gtggtagaag ttccgacaga ccgcatcgtc ttggttggaa ccaaaccagt agctcaagaa
     6661 gctaaaaaac cacaagtgtc agaaaaagca gatacaaaac caattgattc aagtgaagct
     6721 agtcaaacta ataaagccca gttaccaagt acaggtagtg cggcaagcca agcagcagta
     6781 gcagcaggtt taactcttct aggtttgagt gcaggattag tagttactaa aggtaaaaaa
     6841 gaagactaga aattttgata ccttctttat cattaaagac caaagaaaaa ctcttgtcct
     6901 aaatagaaga taaagagcct gagacaaaat agttctcaac cacaaaaaag ctagatattt
     6961 ccaattgtgg aactctagct ttttaatttt gagtcattct cttattgtat agtagattga
     7021 aactagaata gtacactttt acttctaaaa cattgttaga aatcgatttg attgtcctta
     7081 tcgatttgtc ctgttcttat ttcattttac tatattttag gaggttattg gccataagta
     7141 ccaatcccat gtcaattctc acttgacgct tccctctcag attacatctc ttgtaaccca
     7201 aagaagcctt tatctgccca aagacaggtt ccacatcaat cttgcgttga gcgaaaatct
     7261 gtctaccttg gggagataaa agcgcctgac attctttagc tttcaagttt tgatagcgtt
     7321 cgttcatata cagtcccttt tgaggggctg attcaggttc gtcggcgtag taaaccttga
     7381 tttcttgttg aaagtctgtc tgtgttttct gatatttggt atggtgaaaa cgataatacc
     7441 agccatcagg atgtgtgtag ctatcctcct tgtcattata gtgccaattc gctaagtttt
     7501 tagctgactc tttataccct ctcttttgtt tcttatcaaa catggcatat ttaatcagaa
     7561 ggggagtgat ttcttcttga aaatagacct gtatctgttc ttgaagtttg gcggagaatt
     7621 tctctgtcgt tttcttccac acaaaactat acttgttggc attggcttca atcttagttc
     7681 tgtcaataaa caaacaatct aaggtcacta actcttccat ttttcaattt ttcgaccaga
     7741 gaaaatccct tgtgaatagg caaatagaag agtagataca agcattttag ggtgataaga
     7801 cgggcgatca aaggcatgat aggaggtgta gaagtgacgt tcctccaagg tattcaccac
     7861 tttttcaata gtaaaaacga gatgatcttg tggtaagaag gaactgattt ctagtggtaa
     7921 agttgtttga tttgtgttat agtgaatatg catgggatag cctttctaaa tggtttattg
     7981 ggcaattcta ttttaccaag actatcgtaa ccatccaaaa aagcaacggc aaatccgttg
     8041 cctttttgga tataagagac tattgtccca gactcttttc tttgtataaa attgtatagt
     8101 gttattgtct atactatttt gtagagaaag caaaaatccc tagaaaatgc ttagggattc
     8161 gactatttca ttaagaacaa ttcaacaatc atttctatat acatgataag ggttaataag
     8221 aatcctgcac gccagaagaa tttgataaat ttagggtagt aaaagctgcg ttttttcaaa
     8281 aggaagaaaa agacgagaat aatggctagg atcgagaggg ctagccccag tctagggagg
     8341 aaattatggg taaaggtttt agctgtaatc aagtaatact caaataccaa gactggaaaa
     8401 gccaaatccg caaagtttcg tcctagtttt tttaatctaa aaagtttggt taggataatg
     8461 cagacgacta aggtcagtat caataataaa atagatgcta atttcattaa aatcataccc
     8521 atattgtatc ataaaaaaag gctaatggaa acgagaaaca gaggaattta taggaaagtc
     8581 aggcttttga gattgtgggt gatttttgtt ataatgaaag tcatgaaatc ttataatacc
     8641 ttgaatgatt attatcgaaa actctttgga gaaaagactt ttaaagtccc tattgatgcg
     8701 ggatttgatt gtcccaatcg tgatgggact gtggctcatg ggggctgtac tttttgtacg
     8761 gtttctggtt ctggagatgc cattgtggca ccggatgcgc ctatccgtga gcaattttat
     8821 aaggaaattg actttatgca ccgcaagtgg ccggatgttc ggaagtatct ggtttatttt
     8881 caaaatttta ccaacaccca tgaaaaggtg gaagttattc gagagcgtta tgaacaggct
     8941 atcaatgagc caggtgtggt aggaatcaat attggaacgc gcccagactg tttaccagac
     9001 gaaaccatcg aatatttggc tgagttatcg gagtgcatgc atgtgacggt tgaattgggc
     9061 ttgcaaacta cttatgaagc aacctctgac ctgattaacc gtgcgcattc ttatgaattg
     9121 taggtggaaa cggtcaagcg tttgagaaag tatccaaaaa ttgagattgt ttcccatttg
     9181 atcaatggtt tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc
     9241 acggataacg atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt
     9301 atgcaacgag attaccatga ggggcgttta caattgatga gtcaggacga atatgtcagg
     9361 gtcatctgtg accaactgga aattattccc aaacatatcg tcatccatcg aattacagga
     9421 gatgcgccta gggatatgct gcttggtcct atgtggagcc tcaataaatg ggaagtcctc
     9481 aacagcattg agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa
     9541 caggagtttg aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt
     9601 gacaaaagag gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt
     9661 agccaagcta gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac
     9721 ccaagagcgt ttgcatcagg ctgacttgac aaatgcccag ttaatcttgc aaggccatga
     9781 gacactggac cagtttgtga taaaagctaa ggcagggatt tttaatctgg gctatttgcc
     9841 ggcagctgat aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct
     9901 atgtggctta cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg
     9961 aggcgatctc gagagagatg ctgtcttgga ttttgtgatc cagttgaacc aacaagagta
    10021 cacagctgcc atttaccgaa ctttaaacca agtcaacaac ccgccgtttt tagtgatgat
    10081 tgaaaaatta gagagataca gacatggata aacaatactt acacgaaaag ctggatgcca
    10141 tgcgccagaa ttttgttgaa tcaacccacc acgaacgagc gatgggcgtg ctagaccaag
    10201 cgcatatgag caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac
    10261 ggtgccagag aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc
    10321 agaaggagat atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact
    10381 gatttacctc atcctatttt tactggtttt gattgtctca agtacaacca ataagctcct
    10441 gccctttttg cctctccctt tggtgcaaat tcttttggga attgtgattg gtctcttttt
    10501 acccaatact gactttcacc ttaatacgga gttgtttttg gcactggtta tcggaccctt
    10561 gcttttccga gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat
    10621 tgtttatctc atatttccag tgatttttat ctcgaccctg agtttgggtg gcttggccca
    10681 tcttctttgg ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc
    10741 tacggacttg gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc
    10801 caatattctt aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt
    10861 agctttgaca gcttggacaa ctggagcttt ttctctgggg caagctagca gttcgctcat
    10921 cttttcaatc ctaggcggtt ttttaattgg atttttaaca gccatgacca accgcttcct
    10981 ccataccttc ttgctaagtg tgcgcgcaac ggatattgcc agtgaacttt tattagaatt
    11041 gagtttgcct ctagtgacct tctttctggc agaagaagtt catgtttcag gtattattgc
    11101 cgtcgtagtt gctggaattt taaaggcaag tcgcttcaag aaaatcacgc tcctcgaagc
    11161 ccaagtggat acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc
    11221 tgtctttgtg attttaggga tggagttgga aatgatagca gaacctatct tgaccaatcc
    11281 aatctataat cctctacttt tattgctatc tcttatcgcc cttacctttg tcctttttgt
    11341 cattcgtttt attatgatct atggctatta tgcctataga acccgacgcc tcaagaaaaa
    11401 gctaaataag tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt
    11461 gtcgattgct acgattctct tgataccaag taatctagaa caggagtatc ctcttttgct
    11521 tttccttgtt gcaggtgtga cgcttgttag ctttttaaca ggtctcttgg tcttgcctca
    11581 tctttctgat gaagaggaag aaagcaagga ttatcttatg catatcgcca ttttgaatga
    11641 agtaacgcta gagttggaaa aagagttgga agacaccaga aataaacttc ccctctatgc
    11701 ggctattgac aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga
    11761 tgatcaagaa gactgggctg ctttgaaact cttgattctt agtattgaaa gtgatggttt
    11821 ggaacaggcc tatgaagagg ggaacattag caatcgtgct taccgagttt accaacgtta
    11881 tctgaaaaat atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt
    11941 tcttgtttcc ttgaggattt tacgttttct ccttcatgaa gtttttactc tcggaaagac
    12001 cttccgtagc tggaagaaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat
    12061 tgcagagctc tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg
    12121 agtctacaga cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat
    12181 tatcagcagt ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga
    12241 tgaaatgctg agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg
    12301 attgattacg actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta
    12361 ttccttgaag gaagctgcca ataccctacc gtatgatatg gtggaattgg taagaagaaa
    12421 ttagttaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtat
    12481 gtgactgact tcgtcagtct tatctacaac ctcaaaacag tgttttgagc agcttgcggc
    12541 tagctttcta gtttgctctt tgattttcat tgagtataag attataagtg aaggagtatg
    12601 acatgaaaaa gtggtggaaa gagctgatag acaaaccttt attaaaagct tttttgcatt
    12661 attatcaagc atcagatagt gagttgacca gtgtcgcagt agcctactat tggttgattt
    12721 cgattttccc cctgcttttg gtggtggtca atatcctccc ctattttcag attcctgtgg
    12781 gagaattttt gggctttatg aaagacgtcc tgcccccaag cctctatgaa ggtgtggaaa
    12841 aaatagccag agaagtcttg acgcaacctt caacaggttt attgagtttt tctgttttat
    12901 cggcgttatg gagtttttca aaatctatga atttcttgca aaaagctttt aacaaggctt
    12961 atggcgtcga aaagagtcgt ggacttattt cccatcagat gctaagtctc ctagtcagtt
    13021 ttggcttgca gctcctcttt gcgtttgcct tgtttttgat cttatttggt cagatgattt
    13081 tggctttact tgctcattat tggacaaaag atggcattat ctatcaggct ttacaagggt
    13141 tggcaggtcc tctgatttac gccctcctct ttgctatctt ggtcatgctt tattattttc
    13201 ttcccaatct atctaacagg aaaattagct ataccctacc aggcagtgcc tttgttctct
    13261 tagtgatttt aggtttacta actctgttct ctagttatct caattactat gtccatcatt
    13321 tggtggatgt ccgaatttta ggttccgttc ttcttgttgt tatgatgttt tggtttatct
    13381 tgattgctaa aattgttatc ctaggcgcgg tgatcaatgc cagtatgcag agtttgaaag
    13441 atccggtctt taaaaaagac taactattta tccattacaa aagcgcatag tatcaaggct
    13501 ttatagaatc ttgatactat gcgcttttgt gctttgacag ttttttgtat agtttttgtc
    13561 caagatttct aaatcatctg attaaattcc tattttcccc tatcattgtc cctgtttttt
    13621 cgaaaatgga aggagtataa taaatctatc aataaaatga taggaggaac tttatgggtc
    13681 atattttctt ttttctaagt gtctttttgg cagggattct atccttcttt tctccttgta
    13741 tcttaccttt gttaccggtc tatacaggag tgttactaga tgataaggat ggtgctcagg
    13801 cttctagcgg caaattttca atctcagtta ctagtttatt acgaactctg gcctttatag
    13861 caggaatttc ctttatattt attttgttgg gctatggagc tggtttttta ggcgatttgc
    13921 tttatgcttc ttggttccaa tatcttactg gggcaattat tatccttctt ggtttgcacc
    13981 aaatggagat tctacacttt aaggggcttt ataaggaaaa gaggctacaa ctgcaaggac
    14041 aggggcaaaa tggtaagggc tatagtcagg catttttatt gggcttgacc tttagttttg
    14101 cttggacgcc ttgcgtgggg ccggttctgg ggtctgtttt ggccttggcg gcttcaggtg
    14161 gttcaggagc ttggcaggga gctggtctca tgttggtgta tacgctgggc ttggcgctac
    14221 cattcttgct tctagctctg acctctagtt atgttttgaa acatttccga aaacttcatc
    14281 cctatctcgg aatcctcaaa aaagtgggtg gttttctcat tattgtgatg ggcttcttgg
    14341 ttctgtttgg aaatgcttca attttaagtc aattatttga ataaaatgga aaggaatatc
    14401 aatatgaaaa aatggcaaac atgtgttctt ggagcaggtt cgctcctttg tttgacggct
    14461 tgttcaggca agtccgtgac tagtgaacac caaacgaaag atgaaatgaa gacggagcag
    14521 acagctagta aaacaagcgc agctaaaggg aaagaggtgg ctgattttga attgatggga
    14581 gtagatggca agacctaccg tttatctgat tacaagggca agaaagtcta tctcaaattc
    14641 tgggcttctt ggtgttccat ctgtctggct agtcttccag atacggatga gattgctaaa
    14701 gaagctggtg atgactatgt ggtcttgaca gtagtgtcac caggacataa gggagagcaa
    14761 tctgaagcgg actttaagaa ttggtataag ggattggatt ataaaaatct cccagtccta
    14821 gttgacccat caggcaaact tttggaaact tatggtgtcc gttcttaccc aacccaagcc
    14881 tttatagaca aagaaggcaa gctggtcaaa acacatccag gattcatgga aaaagatgca
    14941 attttgcaaa ctttgaagga attagcctag gaggcgtctt atgaatgata agttaaaaat
    15001 cttcttgttg ctaggagtat tttttctagc cataaccggt ttctatgttc tattgatacg
    15061 aaatgcaggg cagacagatg cctcgcaaat tgaaaaggcg gcagttagcc aaggaggaaa
    15121 agcagtgaaa aaaacagaaa ttagtaaaga cgcagacttg cacgaaattt atctagctgg
    15181 aggttgtttc tggggagtgg aggaatattt ctcacgtgtt cccggggtga cggatgccgt
    15241 ttcaggctat gcaaatggta gaggagaaac aaccaagtac gaattgatta accaaacagg
    15301 tcatgcagaa accgtccatg tcacctatga tgccaagcaa atttctctca aggaaatcct
    15361 gcttcactat ttccgcatta tcaatccaac cagcaaaaat aaacaaggaa atgatgtggg
    15421 gacccagtac cgtactggtg tttattacac agatgacaag gatttggaag tgattaacca
    15481 agtctttgat gaggtggcta agaaatacga tcaacctcta gcagttgaaa aggaaaactt
    15541 gaagaatttt gtggtggctg aggattacca tcaagactat ctcaagaaaa atccaaatgg
    15601 ctactgccat atcaatgtta atcaggcggc ctatcctgtc attgatgcca gcaaatatcc
    15661 aaaaccaagt gatgaggaat tgaaaaagac cctgtcacct gaggagtatg cagttaccca
    15721 ggaaaatcaa acagaacgag ctttctcaaa ccgttactgg gataaatttg aatccggtat
    15781 ctatgtggat atagcaactg gggaacctct cttttcatca aaagacaaat ttgagtctgg
    15841 ttgtggctgg cctagtttta cccaacccat cagtccagat gttgtcacct acaaggaaga
    15901 taagtcctac aatatgacgc gtatggaagt gcggagccga gtaggagatt ctcaccttgg
    15961 gcatgtcttt acggatggtc cacaggacaa gggcggctta cgttactgta tcaatagcct
    16021 ctctatccgc tttattccca aagaccaaat ggaagaaaaa ggctacgctt atttactaga
    16081 ttatgttgat taatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta
    16141 tgtagggtta ctgacttcgt cagttttatc tacaacctca aagctatact ttgagctgac
    16201 ttcgtcagtt ttatttacaa cctcaaagcg gtactttgag aaacctgtgg ctatcttcct
    16261 agtttacttt ttgattttta ttgagtataa gaggtctttc ctaagcagtt agaggagaat
    16321 tttgctatac tgatactagt aagtggtaaa ggagcagagc atgacctaca caatcttaat
    16381 cgtagaagat gaatatctgg taagacaagg tttgactaaa ctggtcaatg tagcagccta
    16441 cgatatggaa atcatcggtc aggctgaaaa tggaaggcag gcttgggaat tgatccaaaa
    16501 gcaggtgcca gatatcattt taaccgatat caacatgcct catctaaatg gcatccagtt
    16561 ggccagtctg gtacgagaaa cctatcctca ggttcatttg gtctttttaa caggttacga
    16621 tgattttgat tatgccttgt ctgctgtcaa actaggtgtg gacgactacc tgctcaaacc
    16681 cttttctcgt caggatattg aggaaatgtt ggggaaaatc aaacaaaaac tagacaagga
    16741 agagaaagaa gagcagttac aagatttatt gactaatagg tttgaaggaa acatggccca
    16801 gaaaatccag tctcatctgg ctgatagcca atttagttta aagtctttag ccagtgactt
    16861 aggttttagt ccgacctatc tgagttcctt gattaagaaa gagttgggct tgccttttca
    16921 ggattatctg gtgagagaac gtgttaaaca agccaagctc ttgcttttaa ctacagatct
    16981 gaagatttat gagatcgcag agaaggttgg ttttgaagat atgaactatt ttacccaacg
    17041 ttttaagcag attgcaggtg tgacacctcg tcagtttaag aagggagaag accgatgaag
    17101 cgttcttctc ttttagttag aatggttatt tccatctttc tggtctttct cattctccta
    17161 gctctggttg gaactttcta ctatcaatca agttcttcag ccattgaggc caccattgag
    17221 ggcaacagcc aaacgaccat cagccagact agccacttta ttcagtctta tatcaaaaaa
    17281 ctagaaacca cctcgactgg tttgacccag cagacggatg ttctggccta tgctgagaat
    17341 cccagtcaag acaaggtcga gggaatccga gatttgtttt tgaccatctt gaagtcagat
    17401 aaggacttga aaactgttgt gctggtgacc aaatctggtc aggtcatttc tacagatgac
    17461 agtgtgcaga tgaaaacttc ctctgatatg atggctgagg attggtacca aaaggccatt
    17521 catcagggag ctatgcctgt tttgactcca gctcgtaaat cagatagtca gtgggtcatt
    17581 tctgtcactc aagaacttgt tgatgcaaag ggagccaatc ttggtgtgct tcgtttggat
    17641 atttcttatg aaactctgga agcctatctc aatcaactcc agttggggca gcagggcttt
    17701 gccttcatta tcaatgaaaa ccatgaattt gtctaccatc ctcaacacac agtttatagt
    17761 tcgtctagca aaatggaggc tatgaaaccc tacatcgata caggtcaggg ttatactcct
    17821 ggtcacaaat cctacgtcag tcaagagaag attgcaggaa ctgattggac ggtgcttggc
    17881 gtgtcatcat tggaaaagtt agaccaggtt cggagtcagc tcttgtggac cttgcttggg
    17941 gccagtgtca catctcttct tgtctgtctc tgcttagtgt ggttcagtct taaacgctgg
    18001 attgctcctt tgaaggattt gagagaaacc atgttggaaa ttgcttctgg tgctcaaaat
    18061 cttcgtgcca aggaagttgg tgcctatgaa ctgagagaag taactcgcca atttaatgct
    18121 atgttggatc agattgatca gttgatggta gctattcgta gccaggaaga aacgacccgt
    18181 cagtaccaac ttcaagccct ttcgagccag attaatccac atttcctcta taacactttg
    18241 gacaccatca tctggatggc tgaatttcat gatagtcagc gagtggtgca ggtgaccaag
    18301 tccttggcaa cctatttccg cttggcgctc aatcaaggca aggacttgat ttgtctctct
    18361 gacgaaatca atcatgtccg ccagtatctc tttatccaga aacaacgcta tggagataag
    18421 ctggaatacg aaattaatga aaatgttgcc tttgataatt tagtcttacc caagctggtc
    18481 ctacaacccc ttgtagaaaa tgctctttac catggcatta aggaaaagga aggtcagggc
    18541 catattaaac tttctgtcca gaaacaggat tcgggattgg tcatccgtat tgaggatgat
    18601 ggcgttggct tccaagatgc tggtgatagt agtcaaagtc aactcaaacg tgggggagtt
    18661 ggtcttcaaa atgtcgatca acggctcaaa cttcattttg gagccaatta ccatatgaag
    18721 attgattcta gaccccaaaa agggacgaaa gttgaaatat atataaatag aatagaaact
    18781 agctaactcc cagtcaattc tgggagtttt atgcgtaatt gggcaaactt tctaggttgt
    18841 ctatcttgct aaaaacaaga aaaaacggta tgatagaaga gtgacaacag aggtaacaga
    18901 gatgaacgag acacagattc aaagagaaac acgtcaggta gtggaagatg ttttagaaaa
    18961 gaccaatttg aagcaggggg ccctttttgt tttaggtcta tcttctagtg aggtgctagg
    19021 tggtcatatt gggaaagaat ccagtcaaga gattggtgag ctgatagtgg agactatctt
    19081 ggatattcta ggtagtagag gtatccattt agcagttcaa ggttgtgagc atgtcaatcg
    19141 ggctctcgtt gttgaacgtc aggtggcaga gcagtttggt ctggaaattg tcagtgtcca
    19201 tcctactctt catgcaggag gttcggggca gttggcagcc tttaagttta tgcaggatcc
    19261 agttgaggtt gaatttatca aggctcatgc tggattggat atcggagaca ctgcaattgg
    19321 catgcatgtc aagcatgttc aggttccgat tcgccctatt ttgagagaga ttggtcatgc
    19381 ccatgtaacg gcactggcta gtcgtccaaa attaatcgga ggtgcgcgtg cgcactatcc
    19441 gcaagatgct attaggaagt tttgagaatg aataaaggag ataaaagtgt ttaaaaaaga
    19501 ccgtttttca attcgtaaga ttaagggagt tgtaggctct gtatttcttg gaagcctttt
    19561 gatggctcct tctgtagtgg acgcagccac ctatcactat gtaaataaag agattatttc
    19621 acaagaagct aaagatttaa ttcagacagg aaagcctgac aggaatgaag ttgtatatgg
    19681 tttggtgtat caaaaagatc agttgcctca aacagggaca gaagcatctg ttttgacagc
    19741 ttttggtttg ctgactgttg ggagcttgct tttaatctac aagagaaaga aaattgctag
    19801 cgtctttcta gttggagcta tgggattggt agttcttcct agtgcagggg ctgtagaccc
    19861 agttgcgacc ctagcgctgg ctagtcgaga gggtgttgtt gaaatggagg gctatcgcta
    19921 tgttggttat ctatcaggtg acatcctcaa aacgcttggc ttggacactg ttttagaaga
    19981 aacctcagca aaacctggag aggtgactgt ggtcgaagtt gagactcctc aatcaataac
    20041 aaatcaggag caagctagga cagaaaacca agtagtagag acagaggaag ctccaaaaga
    20101 agaagcacct aaaacagaag aaagtccaaa ggaagaacca aaatcggagg taaaacctac
    20161 tgacgacacc cttcctaaag tagaagaggg gaaagaagat tcagcagaac cagctccagt
    20221 tgaagaagta ggtggagaag ttgagtcaaa accagaggaa aaagtagcag ttaagccaga
    20281 aagtcaacca tcagacaaac cagctgagga atcaaaagtt gaacaagcag gtgaaccagt
    20341 cgcgccaaga gaagacgaaa aggcaccagt cgagccagaa aagcaaccag aagctcctga
    20401 agaagagaag gctgtagagg aaacaccgaa acaagaagag tcaactccag ataccaaggc
    20461 tgaagaaact gtagaaccaa aagaggagac tgttaatcaa tctattgaac aaccaaaagt
    20521 tgaaacgcct gctgtagaaa aacaaacaga accaacagag gaaccaaaag ttgaacaagc
    20581 aggtgaacca gtcgcgccaa gagaagacga acaggcacca acggcaccag ttgagccaga
    20641 aaagcaacca gaagttcctg aagaagagaa ggctgtagag gaaacaccga aaccagaaga
    20701 taaaataaag ggtattggta ctaaagaacc agttgataaa agtgagttaa ataatcaaat
    20761 tgataaagct agttcagttt ctcctactga ttattctaca gcaagttaca atgctcttgg
    20821 acctgtttta gaaactgcaa aaggtgtcta tgcttcagag cctgtaaaac agcctgaggt
    20881 aaatagcgag acaaataaac ttaaaacggc tattgacgct ctaaacgttg ataaaactga
    20941 attaaacaat acgattgcag atgcaaaaac aaaggtaaaa gaacattaca gtgatagaag
    21001 ttggcaaaac ctccaaactg aagttacaaa ggctgaaaaa gttgcagcta atacagatgc
    21061 taaacaaagt gaagttaacg aagctgttga aaaattaact gcaactattg aaaaattggt
    21121 tgaattatct gaaaagccaa tattaacatt gactagtacc gataagaaaa tattggaacg
    21181 tgaagctgtt gctaagtata ctctagaaaa tcaaaacaaa acaaaaatca aatcaatcac
    21241 agctgaattg aaaaaaggag aagaagttat taatactgta gtccttacag atgacaaggt
    21301 aacaacagaa actataagcg ctgcatttaa gaacctagag tactacaaag aatacaccct
    21361 atctacaact atgatttacg acagaggtaa cggtgaagaa actgaaactc tagaaaatca
    21421 aaatattcaa ttagatctta aaaaagttga gcttaaaaat attaaacgta cagatttaat
    21481 caaatacgaa aatggaaaag aaactaatga atcactgata acaactattc ctgatgataa
    21541 gagcaattat tatttaaaaa taacttcaaa taatcagaaa actacattac tagctgttaa
    21601 aaatatagaa gaaactacgg ttaacggaac acctgtatat aaagttacag caatcgcaga
    21661 caatttagtc tctagaactg ctgataataa atttgaagaa gaatacgttc actatattga
    21721 aaaacctaaa gtccacgaag ataatgtata ttataatttc aaagaattag tggaagctat
    21781 tcaaaacgat ccttcaaaag aatatcgtct gggacaatca atgagcgcta gaaatgttgt
    21841 tcctaatgga aaatcatata tcactaaaga attcacagga aaacttttaa gttctgaagg
    21901 aaaacaattt gctattactg aattggaaca tccattattt aatgtgataa caaacgcaac
    21961 gataaataat gtgaattttg aaaatgtaga gatagaacgt tctggtcaag ataatattgc
    22021 atcattagcc aatactatga aaggttcttc agttattaca aatgtcaaaa ttacaggcac
    22081 actttcaggt cgtaataatg ttgctggatt tgtaaataat atgaatgatg gaactcgtat
    22141 tgaaaatgtt gctttctttg gcaaactaca ctctacaagt ggaaatggct ctcatacagg
    22201 gggaattgca ggtacaaact atagaggaat tgttagaaaa gcatatgttg atgctactat
    22261 tacaggaaac aaaacacgcg ccagcttgtt agttcctaaa gtagattatg gattaactct
    22321 agaccatctt attggtacaa aagctctcct aactgagtcg gttgtaaaag gtaaaataga
    22381 tgtttcaaat ccagtagaag ttggagcaat agcaagtaag acttggcctg taggtacggt
    22441 aagtaattct gtcagctatg ctaagattat ccgtggagag gagttattcg gctctaacga
    22501 cgttgatgat tctgattatg ctagtgctca tataaaagat ttatatgcgg tagagggata
    22561 ttcgtcaggt aatagatcat ttaggaaatc taaaacattt actaaattaa ctaaagaaca
    22621 agctgatgct aaagttacta ctttcaatat tactgctgat aaattagaaa gtgatctatc
    22681 tcctcttgca aaacttaatg aagaaaaagc ctattctagt attcaagatt ataacgctga
    22741 atataaccaa gcctataaaa atcttgaaaa attaatacca ttctacaata aagattatat
    22801 tgtatatcaa ggtaataaat taaataaaga acaccatcta aatactaaag aagttctttc
    22861 tgttaccgcg atgaacaaca atgagtttat cacaaaccta gatgaagcta ataaaattat
    22921 tgttcactat gcggacggta caaaagatta ctttaacttg tcttctagca gtgaaggttt
    22981 aagtaatgta aaagaatata ctataactga cttaggaatt aaatatacac ctaatatcgt
    23041 tcaaaaagat aacactactc ttgttaatga tataaaatct attttagaat cagtagagct
    23101 tcagtctcaa acgatgtatc agcatctaaa tcgattaggt gactatagag ttaatgcaat
    23161 caaagattta tatttagaag aaagcttcac agatgttaaa gaaaacttaa caaacctaat
    23221 cacaaaatta gttcaaaacg aagaacatca actaaatgat tctccagctg ctcgtcaaat
    23281 gattcgtgat aaagtcgaga aaaacaaagc agctttatta ctaggtttaa cttacctaaa
    23341 tcgttactat ggagttaaat ttggtgatgt taatattaaa gaattaatgc tattcaaacc
    23401 agatttctat ggtgaaaaag ttagcgtatt agacagatta attgaaatcg gttctaaaga
    23461 gaacaacatt aaaggttcac gtacattcga cgcattcggt caagtattgg ctaaatatac
    23521 taaatcaggt aatttagatg catttttaaa ttataataga caattgttca caaatataga
    23581 caatatgaac gattggttta ttgatgctac agaagaccat gtctacatcg cagaacgcgc
    23641 ttctgaggtc gaagaaatta aaaattctaa acatcgtgca ttcgataatt taaaacgaag
    23701 tcaccttaga aatactatac tcccactact gaatattgat aaagcacatc tttatttaat
    23761 ttcaaattat aatgcaattg cctttggtag tgcagagcga ttaggtaaaa aatcattaga
    23821 agatattaaa gatatcgtta acaaagctgc agatggttat agaaactatt atgatttctg
    23881 gtatcgtcta gcgtctgata acgttaaaca acgactacta agagatgctg ttattcctat
    23941 ttgggaaggt tataacgctc ctggtggatg ggttgaaaaa tatggccgct ataataccga
    24001 caaagtatat actcctctta gagaattctt tggtcctatg gataagtatt ataattataa
    24061 tggaacagga gcttatgctg ctatatatcc taactctgat gatattagaa ctgatgtaaa
    24121 atatgttcat ttagaaatgg ttggtgaata cggtatttca gtttacacac atgaaacaac
    24181 acacgtcaac gaccgtgcga tttacttagg tggctttgga caccgtgaag gtactgatgc
    24241 tgaagcatat gctcagggta tgctacaaac tcctgttact ggtagtggat ttgatgagtt
    24301 tggttcttta ggtattaata tggtatttaa acgcaaaaat gatgggaatc agtggtatat
    24361 tacagatcca aaaactctaa aaacacgaga agatattaat agatatatga agggttataa
    24421 tgacacttta actcttcttg atgaaattga ggctgaatct gtgatttctc aacaaaataa
    24481 agatttaaat agtgcatggt tcaaaaaaat agatagagaa taccgtgata acaataaatt
    24541 aaatcaatgg gataaaattc gaaatctaag tcaagaagag aaaaatgaat taaatattca
    24601 atctgttaat gatttagttg atcaacaatt aatgactaat cgcaatccag gtaatggtat
    24661 ctataaaccc gaagcaatta gctataacga tcaatcacct tatgtaggtg ttagaatgat
    24721 gaccggtatc tacggaggta atactagtaa aggtgctcct ggagctgttt cattcaaaca
    24781 taatgctttt agattatggg gttactacgg atacgaaaat gggttcttag gttatgcttc
    24841 aaataaatat aaacaacaat ctaaaacaga tggtgagtct gttctaagtg atgaatatat
    24901 tatcaagaaa atatctaaca atacatttaa tactattgaa gaatttaaaa aagcttactt
    24961 caaagaagtt aaagataaag caacgaaagg attaacaaca ttcgaagtaa atggttcttc
    25021 cgtttcatca tacgatgatt tactgacatt gtttaaagaa gctgttaaaa aagatgccga
    25081 aactcttaaa caagaagcaa acggtaataa aacagtatct atgaataata cagttaaatt
    25141 aaaagaagct gtttataaga aacttcttca acaaacaaat agctttaaaa cttcaatctt
    25201 taaataattg aaatctctca tctgctttgc ggatgagggt tttcttattt tatgctatac
    25261 ttaaggtatg catagaaaaa cagtgattga ttttaggact ttgggggaga gatatacctt
    25321 tacccagcct attaaagagt tgaaaacgag aaatgtagaa gaagtggcag atttgctggc
    25381 acaagtggaa agctaccaag agcaaggtta ttatgtggtg gggtatgtca gctacgaggc
    25441 tgcacctgct tttgaggaaa aattagcagt tcacaaggat cccctactgg gagagtacct
    25501 gctttacttt actgttcatg atatcgtaga aacttcccct attcctctga cttatgagga
    25561 tattgatttg ccctcaaatt ggcaggaagt aacgtctgca gcagactatg aaaaggccat
    25621 tgcccaaatt caccatcatt tacggcaggg agatacctat caggtcaact acaccgtcca
    25681 acttaagcaa aagttaaatg ccaatccttt tgccatctac aatcgtatgg tggtagagca
    25741 ggaggcgggc tacaatgcct atgtggaaca tgacgagatg acagtgattt ccatgagccc
    25801 agagctcttt tttgagcaaa atgatcgcga gttgacaaca cgaccaatga aggggacgac
    25861 tcagcgtggg gtaactgacc aagaagatct tgaacaggcc agttggttgg aacaggatcc
    25921 caaaaatcgc tctgaaaata tgatgattgt ggacctcttg cgcaatgata tgaaccgtat
    25981 ttctgaagtt gggagcgagc acgtggagcg tctgtgtcag gtagagcagt attcaactgt
    26041 ttggcagatg acttcgacca tcaagagtca gttgcgagag gatgtggacc ttgttgaaat
    26101 cttccgctca ctctttcctt gtggttccat aacgggtgca ccgaaaattg cgacaatgga
    26161 gattatcaag gacttggagc ctcaaccgcg tggagtctac tgtggaacga ttggtctctt
    26221 gcttccaaat ggacgacgga tttttaatgt ggccattcgt accattcaac ttcacaaagg
    26281 tcaagccatt tatggagttg gcggagggat tacttgggat agcacatggg aatctgaata
    26341 ccgagaggtt catcaaaagg cagcagttct ctatcgtaaa caagctcgct tccaattaat
    26401 tacaactgga aaaatcagcc aaaaacaact gatgtttgaa gaacaacatc tggaaaggct
    26461 gagaaaagct agtcgttatt ttgcttttcc ttttgatgca gaagacttgg gacacaagat
    26521 tgaggaagag tgtcaggatt gtgaagctaa tcgagattac cgcttgcgaa tcagccttag
    26581 taaatctgga gagatagaag tcaatcgtca agtattaacc cctctcagta caagcttttg
    26641 tcaggcccaa gtctgtcttc aggaagctgc tttgaatcaa tcctttacct actttaaaac
    26701 cactcaccga ccgcatttga gcctaggaga acaagagaag atttaccaca ataagtcagg
    26761 agaactgctt gaaacctcta taggaaattt ggttctgaaa atcgctggaa aactctacac
    26821 accgcctatc cgacttggaa tcttgccagg aatttaccgt cagcatttgc tagaaacagg
    26881 acaggtagaa gagaaagtct tgaccttggc agatttaacc caagcagaag ctatttacgg
    26941 ctgtaatgca gtgagaggct tgtatgaatt aagccttgag gagaactaga tgaaattaat
    27001 atttttgcat gggttagggc agtcagcaga gtcttggaaa gaagttcgga atctactgac
    27061 agattatcct tctgaggcta tcgagttatt tccttctgga gttagtaact atcaacaagc
    27121 taaagagcga gtttatcagc acctagctca agagacagaa ccttttgttt tgattggatt
    27181 gtccttaggt gctgccctga cactagaact ttccagttac gatttaccaa atcttcgagc
    27241 tttgattctg tcaggatgcc cgcttaaatt agcagggaat atcctatttt atcttcaatt
    27301 gctgattttt aaactacttc ccaaaagagt atttgaaaaa cagggagcag acaagactct
    27361 tatggtcgga gtttctgagg agttgaagac acttgattta acggatatag caggaactta
    27421 cccttatcca accttactaa tttgtggcag caaagataaa ccgaatctta gttctatgaa
    27481 agctctccat agactgttaa cagattcgca gtttcagatc attcctgatg gccctcatgt
    27541 cttgaataag gcaaaaccaa aggagtttgt agaaaagacc agaagtttcc ttgaattgct
    27601 gaaataagtt tgataaaata atattgaaat cgatatcata attatgggag aaagaaatga
    27661 ataaacgtct attttcaaaa atgagtctgg tgacgttgcc aattttagcc ttgttttcac
    27721 aatcagtttt ggcggaagaa aacatccatt tttcgagctg taaggaagct tgggcgaatg
    27781 gctattcgga tattcacgag ggagaacctg gttattctgc caagttagac cgtgatcatg
    27841 atggtgtggc ttgcgaattg aaaaatgctc ctaagggtgc ttttaaagca aaacagtcaa
    27901 cggctattca aatcaacaca agttcagcaa caacaagtgg ttgggttaag caggacggcg
    27961 cttggtacta ctttgatgga aatggaaatc tagtgaaaaa tgcatggcag ggaagctatt
    28021 acctgaaagc tgatggtaaa atggcacaga gtgaatggat ttatgactct tcttatcaag
    28081 cttggtatta tttgaaatca gatggttctt atgcaaaaaa tgcatggcaa ggagcttatt
    28141 accttaaatc aaacggtaaa atggcacaag gtgagtgggt ttatgattct tcttaccaag
    28201 catggtatta cttgaaatca gatggttcat atgctcgcaa tgcatggcaa ggaaactact
    28261 atttgaaatc agatggtaaa atggctaaag gtgaatgggt ttatgatgcc acctatcaag
    28321 cttggtatta tttgacatca gatggttctt atgcttacag tacatggcaa ggaaattact
    28381 atctaaaatc ggatggtaaa atggctgtca atgaatgggt tgatggtgga cgttattatg
    28441 ttggcgctga cggagtttgg aaggaagttc aagcaagtac agcttcttct agtaatgata
    28501 gcaatagtga atattctgct gctttaggaa aggcaaaaag ttataattcg ttattccaca
    28561 tgtcaaaaaa acgtatgtat agacaattaa cttctgattt tgataaattt tcaaatgatg
    28621 cagctcaata tgccattgat catttagatg attaacagat actggtaggc gaaaaaattc
    28681 cttataattc attcacgcaa acctttgcat aagtgaatgg attttgatat actagtactg
    28741 taagcatttt caattactac acaatgaaaa aggagaatat gatgagtcaa aagattattg
    28801 ggattgacct tggtggaact tctatcaaat ttgcaatctt aacaacagca ggagaaatcc
    28861 aaggaaaatg gtcaatcaag accaacattt tggatgaggg aagtcatatc gttgatgata
    28921 tgattgagtc tattcaacat cgtttggact tgcttggatt ggcagcagcg gacttccaag
    28981 gcattggaat gggatcacca ggtgtggttg accgtgacaa agggactgtt atcggtgcct
    29041 acaacttgaa ctggaaaacc cttcaaccaa tcaaacaaaa gattgaaaaa gctttgggca
    29101 ttccattttt catcgataat gatgccaacg tagcagctct tggtgagcgc tggatgggtg
    29161 ctggagataa ccaaccagac gttgtcttta tgacactcgg tactggtgtt ggtggtggta
    29221 ttgtcgcaga aggcaaattg cttcatggtg ttgctggtgc agcaggtgaa cttggtcata
    29281 tcactgttga ctttgaccaa ccaatctcat gtacttgtgg taaaaaaggc tgccttgaga
    29341 cagttgcttc agcaacaggg attgtcaact tgactcgtcg ctatgccgat gaatacgaag
    29401 gcgatgcagc cttgaaacgc ttgattgata acggagaaga agtaactgct aagactgtct
    29461 ttgatctcgc aaaagaagga gacgaccttg ctttgattgt ttaccgtaac ttctcacgtt
    29521 acttgggaat cgcttgtgct aacattggtt caatcctaaa cccatcaaca atcgtcatcg
    29581 gtggtggtgt gtcagctgcg ggagaattcc ttctacaagg tgttcaaaaa gtttacgatg
    29641 aaaatagttt cccacaagta cgcacatcta ctaaattagc tcttgcaact ctaggaaatg
    29701 acgctggagt tatcggagca gcatcacttg tattgcaata agataatgag agaggaaaat
    29761 gctaacctag agttagtaat gttcctcttt tattttttat gctatactag ttaggacaat
    29821 gaatgaggtg aaacgaaatg acaaaagcag atacgatttt taaagagaat attgaacgca
    29881 ttctaaagga aggtgttttc tcagagcagg ctcgtcctaa gtacaaggat gggactgttg
    29941 ctaattccaa gtatgtaact ggtgcctttt ccgagtacga cttatctaaa ggagaatttc
    30001 ctatcacaac cttgcgtccc attgcaatca aatccgccat caaagaagtt ctctggattt
    30061 accaagacca gtcaaatagc ttagaagtgc ttaatgacaa gtacaatgtt cactactgga
    30121 atgactggga agttggagac acgggaacca ttggtgagcg ctatggtgcc gttgttaaga
    30181 aacacgacat tatcaataag cttctcaaac agttggaaac caatccttgg aaccgccgca
    30241 atattatttc gctctgggat taccaagctt tcgaagaaac agatgggctg ctcccgtgcg
    30301 cctttcagac catgtttgat gttcggcgtg ttgatgggga aatctatctg gatgcgacct
    30361 tgacccagcg ctccaatgat atgctggtgg cccaccacat caacgctatg cagtatgtgg
    30421 ctttgcagat gatgattgcc aaacattttg gctggaaggt tgggaagttc ttctacttca
    30481 tcaacaacct ccatatctat gataatcaat ttgaacaagc tcaggaattg ctccgtcggg
    30541 agccgtcaaa ctgccaacca cgcttggttt taaatgttcc tgatgggact aatttctttg
    30601 atatcaaagc agaagatttt gagttggtgg attatgaccc tgttaagcca cagttgaagt
    30661 ttgacctagc tatttaaaag aatagaaaaa agaagttgag aataatccca acttcttttg
    30721 tttcttaacg tgatacgcgg cgacgagctg cttttttacg gttttcttcg atgaaagctg
    30781 ctttttgctc ttctggttcg attactttct ttttaaatgc gtatactgca cctgcaacgg
    30841 cagcgacagt tcctgcgaca cctgttacaa gacctttagc gaatccttta gccatgagtc
    30901 ttcctccttt atattctcaa tcagccagcc tcctcaagag gtcacatttt tctgactgac
    30961 ctttttgtgt tataataata gtaacgaaaa aatgggaatt tttcaaggaa aaaagatgaa
    31021 aacaaaaata attgtgattg ttggaccgac tgctgttgga aagacggctc tagctattga
    31081 agttgcaaag cgttttaatg gcgaagtggt tagtggagat agccagcaag tctatcgagg
    31141 acttgatatt gggacggcca aggctagtcc agaagagcag gcagctgttc ctcatcattt
    31201 aatcgatgtt agagagataa ccgagtctta ctcggctttt gattttgttt cagaagctaa
    31261 gatgactatt gagggtattc acaaccgtgg caagctagcc attatcgccg gtgggactgg
    31321 actttatatc cagagcttgc tagaaggtta ccacctaggt ggggagactc ctcatgagga
    31381 gattttagct tatcgagcta gtttggagcc atattcagat gaggaattag cccatttggt
    31441 ggatcaagca ggccttgaga ttccccagtt taatcgtcgt cgtgctatgc gtgccttaga
    31501 aattgcccat tttggtcagg atttggaaaa tcaagagacc ttgtatgaac cgctgattat
    31561 ctgcttggat gatgaacgta gtcaacttta tgagcgtatc aaccaccgag tggatttgat
    31621 gtttgaggct gggcttttgg atgaggccaa gtggctgttt gaccattccc ctaatgtaca
    31681 ggctgctaaa ggaattggct ataaggaact ctttccttat ttccgtggag agcagacctt
    31741 ggaggaagct agtgagagtc ttaaacaggc gactcgtcgt tttgccaagc gtcagctgac
    31801 ctggttccgt aatcgcatgc aggtcacctt ttatcagatt ggagaatctg gtgtacaaga
    31861 ccgcatttta agccagatag aggagttttt agatgattga aacggagaaa aaagaggagc
    31921 gagtcctgct gattggtgtg gaattgcagg gtatggacag ttttgacctc tccatggaag
    31981 aattggctag tttagcgaaa acggcagggg cagtcgttgt agatagctac agacaaaaac
    32041 gtgaaaaata tgattccaag accttcgtcg gctctggtaa gttggaagag attgcgctta
    32101 tggtggatgc agaagaaatc actactgtca tcgtcaacaa tcgtctgacc ccaaggcaga
    32161 atgtcaatct agaggaagtt ctcggtgtta aggtcattga ccgtatgcag ttgattttgg
    32221 atatctttgc catgcgggct cgaagccatg aagggaagct ccaagtccac ctagcccaac
    32281 tcaaatacct tttgcctcgc ttggttggtc aggggattat gctcagccgt caggcagggg
    32341 gaattggttc ccgtggtcct ggtgaaagcc agctggagct gaaccgtcgt agcgttcgca
    32401 atcaaatcac ggatatcgag cgccagctca aggtggttga gaaaaatcgt gcgactgtca
    32461 gagaaaaacg tttggagtct agcactttta agattggttt gattggttat actaatgctg
    32521 ggaaatcaac tatcatgaac atcttgacca gtaagaccca gtatgaagca gatgagctct
    32581 ttgcgactct ggatgcgaca accaagagta ttcatctggg aggcaatctc caagtaactt
    32641 tgacagatac cgttggcttt atccaagatt tgccgacaga gttggtgtcc agtttcaagt
    32701 caaccttgga agaaagcaag catgtggacc ttctggttca tgttatcgat gctagcaatc
    32761 cttaccacga ggagcatgaa aaaacggttc tctccatcat gaaagacctg gacatggaag
    32821 atattcctca cttgacgctt tataataaag cggatttggt ggaggatttc acgcctaccc
    32881 aaacgccata taccctcatt tctgccaagt ctgaggacag tcgtgaaaac ttgcaagcat
    32941 tattgctaga taagattaag gaaatttttg aagcatttac cctgcgagtg cctttttcaa
    33001 agtcctacaa gattcatgat ttagagagtg ttgcaattct ggaagaacgt gattatcagg
    33061 aagacggcga agtgattaca ggctacattt ccgagaaaaa taaatggagg ttagaagaat
    33121 tttatgactg atattaaaac tttggctcta aagtatggag gttatacaag tctggacaag
    33181 gtctatctgg atcagcttct agctggtaaa acagatcagg agcagttaac acttattaca
    33241 cctccgccaa gtgttgttaa tgcctacttt gcagaactct atcagaaaaa gagtcctcag
    33301 gctgcgacgg attattttgc agaactcagt caggaactga atctttacaa tactgagcca
    33361 agtttcaccc tagaaaataa gccttttatt cggcttaatc tgtctggtaa atcctttggt
    33421 ttttgttatg agagtgacgg ccttggtcgg attttctttg aaaatgagga gaaaatctcg
    33481 gatgacttgc tctttgaaat tgcgcaaatt ttcccccatc aattagtctt tgaagagtct
    33541 ggtaagattt acatgaagcc tgtcgaggac gaggaagtta tcagcctaga aagtctcaca
    33601 gctttgactg atttggaaag attggctgat ggtcgaaaac gtctcaaagg ctacagccaa
    33661 gaggatttat tgcaagaagc tgctgctttt tctggcaaac gctatttccg atcggaaaac
    33721 cgcacagcca tgttatatat tgattaatta gaaagtatcg aatggatatt caatttttag
    33781 gaacgggggc tggtcagccc tctaaagccc gcaacgtttc aagtctcgcc ctgaaacttt
    33841 tggatgagat taacgaagtt tggctctttg actgtggaga aggtacgcaa aatcgcattc
    33901 tggaaaccac aattcgacca cgtaaggtca gcaaaatctt tattacccat ctgcatggag
    33961 accacatttt tggtttgcca ggtttccttt ctagccgtgc ctttcaggcc aatgaagagc
    34021 agacagattt ggaaatctac ggacctcaag gaatcaagtc atttgtctta accagccttc
    34081 gtgtgtcagg ttctcgtctg ccctaccgca ttcatttcca tgagtttgac caagattctc
    34141 tgggtaaaat tcttgaaacc gataaattca ctgtgtatgc agaggagctg gaccacacta
    34201 ttttctgtgt tggctatcgt gtcatgcaaa aggatctaga agggacgctg gatgctgaaa
    34261 aactcaaggc tgctggtgtt ccgttcggcc cgctttttgg taaaatcaaa aacggccagg
    34321 atcttgtttt ggaagacgga actgaaatca aggcagcaga ctatatctca gcgccacgtc
    34381 caggtaagat tatcactatt ttaggagaca ctcgaaaaac gggtgccagt gtgcgtctgg
    34441 ctgtcaatgc agatgtccta gttcatgagt ccacttatgg caagggtgat gaaaaaattg
    34501 ctcgtaacca tggtcactca actaatatgc aagctgcaca agtagcggta gaagcaggtg
    34561 ccaaacgcct cctactcaac catatcagtg cccgtttcct ctcaaaagat attagcaaac
    34621 tcaagaaaga cgctgccaca atttttgaaa atgtccatgt ggtcaaagac ttggaagaag
    34681 tggaaatcta gcagtcacag aaaggataag tatgcctact attctcatta ccggagctag
    34741 cggtggtcta gctcaagaaa tggtcaaact cctgcccaat gaccaactca tcttgcttgg
    34801 tagaaataag gaaaaattag cccaactcta cggaaattat tcccatgcag aattgattga
    34861 aattgatatt accgacgatt cagccctaga agctctggta actgatcttt atctccgcta
    34921 tggcaagatt gatgtcttga ttaacaacgc tggttacggg atttttgagg gatttgacca
    34981 gattgctgat aaagatattc accagatgtt tgaggtcaat acctttgccc tgatgaatct
    35041 gtctcgtcac cttgcggctc gtatgaagga aagcagcaaa gggcatatca tcaacatcgt
    35101 cagcatggca ggtctaatag ctactggcaa gtctagtctt tactcagcga ccaagtttgc
    35161 ggctattggt ttttcaaatg ctctgcgact cgaacttatg ccctatggag tctatgtgac
    35221 aacagtcaat ccaggaccaa tccgtacagg atttttcgac caagccgacc cagatggaac
    35281 ttatcttaaa tcggttgatc gcttcctgct agaaccagat gcagtggcta aaaagattgt
    35341 caagattata ggaaaaaaca aacgagaact caatctcccg attttgttga acctagccca
    35401 taagttttat actctctttc ccaagctagc tgataagttg gcaggggaaa cttttaatta
    35461 taagtaaaaa gaaccaatgt gcaggttgtt gctagcctac atattggttc ttatcatttc
    35521 tcaactatca aactgaactt cttctaggag atagtctata aagcgttcgc ccatcttaga
    35581 taggctggtt ttctcatgct ggatatagac cagctcgatc gggtcatcaa tatccagtgg
    35641 gatagaaacg atattgtctc cgtttaggtt gctgttcaaa atccctgtcg caatggtata
    35701 accatccaaa ccaatcaaga gattaaagag ggtggcacgg tcactgacca caatggattt
    35761 cttgtggtgt tcttgagaaa gaatctcttc tgaaaagtag aaggagttgt gcgtcccttg
    35821 gtcatagctg aggtaaggga aattctccaa atcagacagt ttcaccttgt ctttctttgc
    35881 cagagggttg gtcttgctga caaagatatg cggttgcgct gtgaagagat ggtgggctag
    35941 caggtgattg tcatccagca tcttggttaa aacatcacgg ttgtaactgt ttaagaagag
    36001 gaccccgacc tcactgcgga agttcttgac gtcgtcgata atctcccaag tccgagtttc
    36061 acgaaggaag agttcgtatt tctccatatc gcttttcttg agcaaagaga caaaggcatt
    36121 gaccacaaag gcatagtgtt gagacgaaac gctaaagagt tcgcggtggg cgacaggatt
    36181 tttatagcgt tcctccagaa gctgggtctg ctcgacaacc tgacgggcat aagagagaaa
    36241 ctccatgcca tcacgggtca aggtgattcc cttgggattg cggataaaga tctcaatgcc
    36301 catttcattt tccaaatctc gcactgcatt ggagagactt ggctgagtga taaagagttg
    36361 cttggctgcc tcattcatgg agccagtttc gacgattttg ataatataat gtaattgttg
    36421 aattctcatg tttttattgt accatacttg ccttagaatc gctaaagaag ataggaaaat
    36481 aaggagcatt gtgtgagtct cttcttgaaa aaaacacaaa attttgctag aatgagtctt
    36541 atgaaaacat tctatgatgt gcagcaattc ctcaaacgat ttggtattat tgtttacatg
    36601 ggaaaacgct tatatgatat tgaactgatg aagttggaac tatctcggat ttacgatgca
    36661 gggttgatgg ataaattaga ctatctagag gcagaagcag ttcttcgcag agagcacaag
    36721 gtagaattgg attatattga gaaaaatgga gaaaagaact aatggttact tggattttgt
    36781 gggcacttat actagcaatg ttggcgtgga tgggctttaa ctatcttcgt attcgccgtg
    36841 cggctaaaat tgtggacaat gaggagtttg aagccttgat tcgtacgggt caattgattg
    36901 atttgcgcga cccagcagaa ttccacagaa aacatatcct tggtgcacgc aatattcctt
    36961 caagtcagtt gaaaactagt cttgcagccc ttcgtaaaga taaacctgtc cttctctacg
    37021 aaaaccaacg tgcgcaacga gttacaaatg cagctcttta cttgaaaaaa caaggttttt
    37081 ctgagattta tatcctttct tatggcttgg attcttggaa agggaaagtg aagactagct
    37141 aaccttattt ccaaattagt cgaatgatag gatgaagcta ttgttttata gtataattaa
    37201 tttgtataaa atttaaggag ttcttagaac ataaaagaaa agaagaaaaa tccacttttt
    37261 gtgggtatct tgtcgattat tcttggtttg ttatttccaa ttgtaggcct gattttgggt
    37321 atcataggat tggtcttggc tatttcatac caaaaagagt cacaattaga ctataaaata
    37381 gaaaagattc ttaatatctt aggaattgtg atttctgtag ttaactggat tgtagcaatc
    37441 gccttaattt ttcggtaaat aaaagaaaaa agctttagtt ttaaagcttt ttttgtttta
    37501 tcgactcatc tctaaatagt ttttaatgat ttgtaactct ttttggttca aagggcggta
    37561 gttaccttct gctaaatctg ggttcaatgt gaagtcccca aattggattc ttttgaggct
    37621 agtcacctta acaccaaccg agaggaacat tttcttgatt tgatgaaatt ttccttctga
    37681 aatggtgata gaggcttgac tgagggaggg acttgcagat agaatctcta gttttgcggg
    37741 tttacagaca gtgtcatcta aaaagacaat tcctttttga aaggtttgga tatggtcagg
    37801 tgttagaagt ccattaacct caacttggta agtcttatcg acatgatatt ggggatggag
    37861 gagctgaaag cccaagggac cgttatcggt caagaggagg agtcccgttg tatctcggtc
    37921 cagtcggcca acggcataga gcttgtcaga ctggatgtta gatggaagca ggtccatgac
    37981 ggtcggaagt tccttgtctt tgttggctgt aacggcacca gcaggtttat gaagcataag
    38041 ataggtgtgt tcatagcctt gaatgattcg atcctgaaaa aggagttctt gtagtcctgt
    38101 atcgatattt tgagctaggg agcgggctgg gcaaccatcg actagaattt cccctctgag
    38161 gagtgcttgc ttcatggcct ttcggctgat tttttcttga gctaataaat tatctaaacg
    38221 catactgtgc ctatcttatc ataagtcgct cgctttgaaa aggcttgacg tgaaaagaaa
    38281 agcagagtga aaacatttac acttgcttga gttatgttat ttatttgaaa ttatggtata
    38341 atcgttcagt tagaaaataa attttgaata ttatagagga aatcatgaca aaattaagag
    38401 aagatatccg taacattgcg attatcgccc acgttgacca cggtaaaaca accctggttg
    38461 acgaattatt gaaacaatca gaaacgcttg atgcacggac tgaattggca gagcgtgcta
    38521 tggactcaaa cgatatcgaa aaagagcgtg gaattaccat ccttgctaaa aatactgccg
    38581 ttgcttacaa cggaactcgt atcaacatta tggacacacc aggacacgcg gactttggtg
    38641 gagaagttga gcgtatcatg aaaatggttg acggtgttgt cttggtcgta gatgcctatg
    38701 aaggaaccat gccacaaact cgtttcgtat tgaaaaaagc cttggaacaa gaccttgtcc
    38761 caatcgtggt tgttaacaaa atcgataagc catcagctcg tccagcagaa gtagtggatg
    38821 aagtcttgga acttttcatc gagcttggtg cagatgacga ccagcttgat ttcccagtgg
    38881 tttatgcttc agcgatcaac ggaacttctt cattgtcaga tgatccagct gaccaagaag
    38941 cgactatggc accaatcttt gacacgatta tcgaccatat cccagctcca gtagataact
    39001 cagatgagcc tttgcagttc caagtgtcac ttttggacta ca
//
LOCUS       DBDESG010000014        37330 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.014, whole genome shotgun sequence.
ACCESSION   DBDESG010000014 DBDESG010000000
VERSION     DBDESG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 37330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.014"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(28..825)
                     /locus_tag="ACKB38_000694"
                     /pseudo
     CDS             complement(28..825)
                     /locus_tag="ACKB38_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1091..1327
                     /gene="comW"
                     /locus_tag="ACKB38_000695"
     CDS             1091..1327
                     /gene="comW"
                     /locus_tag="ACKB38_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGL7979505.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1558..2844
                     /locus_tag="ACKB38_000696"
     CDS             1558..2844
                     /locus_tag="ACKB38_000696"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGL7979506.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            3045..3512
                     /gene="tadA"
                     /locus_tag="ACKB38_000697"
     CDS             3045..3512
                     /gene="tadA"
                     /locus_tag="ACKB38_000697"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGL7979507.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            3545..3642
                     /gene="ffs"
                     /locus_tag="ACKB38_000698"
     ncRNA           3545..3642
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACKB38_000698"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3699..4142
                     /locus_tag="ACKB38_000699"
     CDS             3699..4142
                     /locus_tag="ACKB38_000699"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGL7979508.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            4144..4659
                     /locus_tag="ACKB38_000700"
     CDS             4144..4659
                     /locus_tag="ACKB38_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGL7979509.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            4673..6034
                     /gene="radA"
                     /locus_tag="ACKB38_000701"
     CDS             4673..6034
                     /gene="radA"
                     /locus_tag="ACKB38_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGL7979510.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            6107..6604
                     /locus_tag="ACKB38_000702"
     CDS             6107..6604
                     /locus_tag="ACKB38_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGL7979511.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            6629..7443
                     /locus_tag="ACKB38_000703"
                     /pseudo
     CDS             6629..7443
                     /locus_tag="ACKB38_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            7588..8556
                     /locus_tag="ACKB38_000704"
     CDS             7588..8556
                     /locus_tag="ACKB38_000704"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGL7979512.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(8690..8971)
                     /locus_tag="ACKB38_000705"
                     /pseudo
     CDS             complement(8690..8971)
                     /locus_tag="ACKB38_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(9098..10005)
                     /locus_tag="ACKB38_000706"
                     /pseudo
     CDS             complement(9098..10005)
                     /locus_tag="ACKB38_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            10261..12894
                     /gene="polA"
                     /locus_tag="ACKB38_000707"
     CDS             10261..12894
                     /gene="polA"
                     /locus_tag="ACKB38_000707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGL7979513.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNVAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYDLAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFVWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            12979..13416
                     /locus_tag="ACKB38_000708"
     CDS             12979..13416
                     /locus_tag="ACKB38_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGL7979514.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            13457..13693
                     /locus_tag="ACKB38_000709"
     CDS             13457..13693
                     /locus_tag="ACKB38_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979515.1"
                     /translation="MKIKEQTRKLATGCSKHCFEVADRTDEVSSKHCFEVVDTTDEVS
                     SNTVLRLQIELTKSVTYTYGKATLTWFEEIFEEY"
     gene            complement(13690..14700)
                     /locus_tag="ACKB38_000710"
     CDS             complement(13690..14700)
                     /locus_tag="ACKB38_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGL7979516.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVVGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            14849..16018
                     /locus_tag="ACKB38_000711"
     CDS             14849..16018
                     /locus_tag="ACKB38_000711"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGL7979517.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAGKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            16015..16785
                     /gene="recO"
                     /locus_tag="ACKB38_000712"
     CDS             16015..16785
                     /gene="recO"
                     /locus_tag="ACKB38_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGL7979518.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            16782..17774
                     /gene="plsX"
                     /locus_tag="ACKB38_000713"
     CDS             16782..17774
                     /gene="plsX"
                     /locus_tag="ACKB38_000713"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGL7979519.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            17780..18013
                     /locus_tag="ACKB38_000714"
     CDS             17780..18013
                     /locus_tag="ACKB38_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGL7979520.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            18059..18349
                     /locus_tag="ACKB38_000715"
                     /pseudo
     CDS             18059..18349
                     /locus_tag="ACKB38_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            18552..18782
                     /gene="blpU"
                     /locus_tag="ACKB38_000716"
     CDS             18552..18782
                     /gene="blpU"
                     /locus_tag="ACKB38_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGL7979521.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            18785..18910
                     /locus_tag="ACKB38_000717"
     CDS             18785..18910
                     /locus_tag="ACKB38_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGL7979522.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            19497..21650
                     /gene="comA"
                     /locus_tag="ACKB38_000718"
     CDS             19497..21650
                     /gene="comA"
                     /locus_tag="ACKB38_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGL7979523.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIF
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLSQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            21663..23012
                     /gene="comB"
                     /locus_tag="ACKB38_000719"
     CDS             21663..23012
                     /gene="comB"
                     /locus_tag="ACKB38_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGL7979524.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            23182..23889
                     /gene="purC"
                     /locus_tag="ACKB38_000720"
     CDS             23182..23889
                     /gene="purC"
                     /locus_tag="ACKB38_000720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGL7979525.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNVAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            23891..24034
                     /locus_tag="ACKB38_000721"
     CDS             23891..24034
                     /locus_tag="ACKB38_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGL7979526.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            24091..27816
                     /locus_tag="ACKB38_000722"
     CDS             24091..27816
                     /locus_tag="ACKB38_000722"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGL7979527.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYAAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGATPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWFKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLA
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELETALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLVGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            27909..29351
                     /gene="purF"
                     /locus_tag="ACKB38_000723"
     CDS             27909..29351
                     /gene="purF"
                     /locus_tag="ACKB38_000723"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGL7979528.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            29570..30592
                     /gene="purM"
                     /locus_tag="ACKB38_000724"
     CDS             29570..30592
                     /gene="purM"
                     /locus_tag="ACKB38_000724"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGL7979529.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            30589..31134
                     /gene="purN"
                     /locus_tag="ACKB38_000725"
     CDS             30589..31134
                     /gene="purN"
                     /locus_tag="ACKB38_000725"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGL7979530.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            31218..31727
                     /locus_tag="ACKB38_000726"
     CDS             31218..31727
                     /locus_tag="ACKB38_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGL7979531.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLLSV"
     gene            31752..33299
                     /gene="purH"
                     /locus_tag="ACKB38_000727"
     CDS             31752..33299
                     /gene="purH"
                     /locus_tag="ACKB38_000727"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGL7979532.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            33421..34683
                     /gene="purD"
                     /locus_tag="ACKB38_000728"
     CDS             33421..34683
                     /gene="purD"
                     /locus_tag="ACKB38_000728"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGL7979533.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHYKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            34940..35056
                     /locus_tag="ACKB38_000729"
     CDS             34940..35056
                     /locus_tag="ACKB38_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGL7979534.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            35086..35574
                     /gene="purE"
                     /locus_tag="ACKB38_000730"
     CDS             35086..35574
                     /gene="purE"
                     /locus_tag="ACKB38_000730"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGL7979535.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            35561..36652
                     /gene="purK"
                     /locus_tag="ACKB38_000731"
     CDS             35561..36652
                     /gene="purK"
                     /locus_tag="ACKB38_000731"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGL7979536.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            36662..36889
                     /locus_tag="ACKB38_000732"
     CDS             36662..36889
                     /locus_tag="ACKB38_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGL7979537.1"
                     /translation="MIQIIVNTFIEKYKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYNVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            36952..>37330
                     /locus_tag="ACKB38_000733"
     CDS             36952..>37330
                     /locus_tag="ACKB38_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HGL7979538.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIAD"
BASE COUNT        10537 a         6900 c         9084 g        10809 t
ORIGIN      
        1 agaggcgaga catttttcgg ttctttgtca actgtagtgg gttgaagaaa gcgaagatct
       61 agaaaggaca aatttcgtcc tttctttttt gaagttttca aagttcctaa aaccaaaggc
      121 attgtgcttg ataagtttga tgagattatt ggtggcttcc agtttggcgt tggaataagg
      181 taattgaagg gcgttgacga ttttctcttt atctttgagg aaggttttaa acaaagtctg
      241 aaacagaggt ggaaaagcaa gagctgatag agattatagt ggtgtttaaa gtcttcggaa
      301 tagctcaaaa gtttatctag aatttcttta ttagtcaagt gcatacgaaa agtagggcga
      361 taaaatcgtt tatcactcag tttctgacta tcttgttgaa tgagcttcca gtagcgcttg
      421 atagccttgt attcatggga tttcggatga tggcttgtgt tctgctctca agaacagtta
      481 tgatattgag tttatcaaag tcctgagcaa taaagctcat ctccatctcc cgattgaaac
      541 agtcactccc cggactgttt caacgtccta ggacataatc tcaggaagac gcgaaaaatc
      601 atgctcaaag tgaaaatcat tgttcttgcg aatgacagtt gaagttgaaa tagacaactg
      661 atgatcaatg tcggtcatag aagtcttttt aattagcttc tgagcaatct tttggttgat
      721 gatacaagga atttgatgat tcttcttgac gatagaagtc tcagcgagct ccatttttga
      781 gcaatgatag cacttaaaac ggccttttct aagaagaatt ctagtttgaa tttttttata
      841 ctagaaaatc agaaccataa tacctatata aaaatattat agttctaata ggatttaccc
      901 aaaagtttta aggcggtctt tttagaactt taattgtttg aaatttaggt agcaaatttg
      961 tttctatttt gtcaactttt cctattttta tcttgttgag gctggtattt taacaattca
     1021 ggaattgata gtgaatgtgt aaaatttttt gttagaataa gtttataaaa aagaaaagga
     1081 gtatttgatt atgttacaaa aaatttatga gcagatggct aatttctatg atagtattga
     1141 agaagagtat ggtcctacat ttggtgataa ttttgactgg gaacatgttc attttaaatt
     1201 tttaatttat tatttagtga gatatggcat tggttgtcgt aaggatttta ttgtttacca
     1261 ttatcgtgtt gcttatcgtt tgtatcttga aaaattggta atgaatcggg gttttatttc
     1321 ttgttgaggt aattttagta aatttccgaa ctaatttact cttttatgga aagatgatag
     1381 taaatagcta gtaatttttc taaatcattt tttaatagtt ggaaatagca aatctttcta
     1441 ttgtttcttc ttgataaaaa ggcgattttt tattataata aattgtaaga tataattgca
     1501 ggtgagagtc ctgccatgta tgtgagaaag gaagagcctg atggctcaga caagattatg
     1561 acttcagttg ttgttgtagg tacccaatgg ggtgatgaag gtaaagggaa gattacagac
     1621 ttcctttcag cgaatgcaga agtgattgca cgttaccaag gtggtgataa tgctggtcac
     1681 acgattgtga ttgacggtaa gaaatttaag ttgcacttga ttccatctgg gattttcttc
     1741 cctgaaaaaa tatctgtcat tgggaatggt atggttgtaa atcctaaatc tcttgtaaaa
     1801 gagttgagct atcttcatga ggaaggtgta acaactgata acttgcgtat ttctgatcgt
     1861 gcgcatgtta ttttgcctta tcatatcgag ttggatcgct tgcaagaaga agctaagggc
     1921 gacaataaga ttggtacgac aattaaggga attggtccag cttatatgga caaggctgct
     1981 cgtgttggaa ttcgtattgc agatctttta gataaagata ttttccgtga gcgtttagaa
     2041 cgtaaccttg ctgaaaagaa tcgtcttttt gaaaaattgt atgacagtaa agcgattgtt
     2101 ttcgatgata tttttgaaga atattacgaa tatggtcaac aaatcaagaa atacgtgata
     2161 gatacatctg ttatcttgaa tgatgcgctt gataatggca aacgtgtgct ttttgaaggt
     2221 gcacaaggtg ttatgctaga tatcgaccaa ggtacttatc catttgttac gtcatcaaac
     2281 cctgtagctg gtggtgtgac aattggttct ggtgtcggtc caagcaagat tgacaaggtt
     2341 gtaggtgtat gtaaagctta tacgagtcgt gtaggagatg gtcctttccc aactgagttg
     2401 tttgatgaag tgggagaacg tatccgtgaa gtgggtcatg aatatggtac aacaactggt
     2461 cgtccacgtc gtgtaggttg gtttgactca gttgtgatgc gtcatagccg tcgtgtttct
     2521 ggtattacta acctttcttt gaactctatt gatgttttga gcggtttgga tactgtgaaa
     2581 atctgtgtgg cctatgatct tgacggtcaa cgtattgact actatccagc tagtcttgaa
     2641 caattgaaac gttgcaagcc tatctatgaa gagttgccag gttggtcaga agatattacc
     2701 ggagttcgca atttggaaga tcttcctgag aatgcgcgta actatgttcg tcgtgtgagt
     2761 gaattggttg gcgttcgtat ttctactttc tcagtaggtc ctggtcgtga acaaacaaat
     2821 attttagaaa gtgtttggtc ctaagagatt tttaagattt gtttaagata ggtcgggtat
     2881 actatagacg gttacaagaa gacctcctaa cttgttgtaa caaatatcct aaacttttct
     2941 ttttcataat aatctcccta tagagtcacc gcattcggtg gctttttttg tgttgggatt
     3001 catgatataa taataaaatc gataagtagg aaaagagaaa agagatgtat tatacgcttg
     3061 aagaaaaaga agtctttatg agggaggctt tgagagaggc tgagattgct cttgaacacg
     3121 atgaaattcc aattggttgt gtgattgtca aagatgggga aatcattggt cgtgggcata
     3181 atgcgcgtga ggaattacag cgagcggtta tgcatgcgga aattatggct atagaggatg
     3241 cgaacttgag tgaggagagc tggcgcttgc tggattgcac actttttgtg accattgaac
     3301 cttgtgtcat gtgtagtgga gcgattgggc ttgcccgcat tccaaatgtg gtctatgggg
     3361 ctaaaaacca gaaatttggc gctgctggga gtttgtacaa tatcttgaca gatgagcgtc
     3421 tcaatcatcg tgtggaggtt gaaataggaa ttttggaaga tgaatgcgca gctatcatgc
     3481 aggatttttt tagaaataga cggaaaaaat aattttgctt ttaaaatgaa taggaatgtg
     3541 atataataaa tagtggagca acagttctgc gtgaagcggg tcaggggagg aatccagcag
     3601 ccctaagcga tttgaattgt gtgctctttt tttcgtgctt tttccgaata aataagatag
     3661 aataatctag aataaatgat aatagaaaag agaaaattat gaaaattcgt ggttttgaat
     3721 tggtttcgag ttttacagat gaaaatttat tgcccaagcg tgagacagcg catgcggctg
     3781 gttacgactt aaaggttgct gtgcgtacag ttgttgcgcc aggagagatt gtcttggttc
     3841 cgacaggggt taaggcttat atgcagccga ctgaggttct ctacctctat gatcgttctt
     3901 caaatcctcg taagaagggc ttggttttaa ttaactcagt tggagtcatt gatggggatt
     3961 attatggaaa tcctggaaat gaagggcata tttttgcgca gatgaagaat atcacagacc
     4021 aagaggttgt tcttgaagtt ggggagcgta ttgtccaggc tgtttttgct actttcttaa
     4081 ttgcagatgg agatgcagct gatggcgttc gaactggtgg atttggatcg acagggcact
     4141 agaatgaaga ttatctttgt acgtcatggg gagccagatt accgtgagtt agaggagcgt
     4201 tcttatatag gatttgggat agatttggca cccttgtctg agatgggacg gcagcaagtc
     4261 cagaaattga gcaaaaatcc tttactctcg tcagctgaaa taatcgtatc ttctgcagtc
     4321 acaagagctt tagaaacggc ttcgtatgtg gtctgtgcta cgggtcttcc tttaagagta
     4381 gagcctttat tacatgaatg gcaggtctat aaaacaggaa tagaaaactt tgaaacagct
     4441 agaagactgt ttttagaaaa caagggggag ttgcttccta atagtcctat tcaatatgag
     4501 acagctacgg aaatgaagtc tcggtttcta gaatgtatgt ctaagtatcg agaacatcag
     4561 actgtggtag ttgttgctca tcgaatgctc atgcgccagt ttgtgccaaa tgagaagatt
     4621 gatttttgcc aagtgattga gtgtgagtta gagatataga aagaggtttg tcatcgcaaa
     4681 gaaaaaagcg acatttgtat gtcaaaattg tgggtataat tcccctaaat atctgggacg
     4741 ttgccccaac tgtgggtctt ggtcttcttt tgtggaagag gttgaggttg ccgaagttaa
     4801 gaatgcgcgt gtgtccttga caggtgagaa aaccaagccc atgaaactag ctgaggtgac
     4861 ttccatcaat gtcaatcgaa ccaagacgga gatggaggaa ttcaaccgtg tgcttggagg
     4921 cggagtggta ccaggaagtc tcgtccttat cggtggggat cctgggattg ggaaatcaac
     4981 tcttctccta caagtctcaa cccagttgtc ccaagtgggg acagttctct atgtcagtgg
     5041 ggaggagtct gcccagcaga ttaaactacg tgcagagcgc ttaggtgata ttgatagtga
     5101 gttttatctc tatgcagaga ccaatatgca gagtgttcgt gcagaagtgg agcgtatcca
     5161 gccagacttt ctcattattg attccatcca gaccatcatg tctcctgaga tttcaggggt
     5221 gcaggggtct gtttctcagg tgcgtgaagt gaccgctgaa ctcatgcagt tggccaagac
     5281 caataacatt gccatcttta tcgtaggtca tgtgaccaaa gaaggaacct tggctgggcc
     5341 tcgtatgttg gagcatatgg tggatacggt gctttacttt gaaggggagc gtcaccacac
     5401 ctttcgtatt ttgagagcgg tcaaaaatcg ttttggttcc actaatgaga ttgggatttt
     5461 tgagatgcag tcgggcggct tggttgaggt actcaatccg agtcaagttt tcctagaaga
     5521 gcgtttggat ggggcgactg gttcctccat cgttgtaacc atggaaggga cgcgtccgat
     5581 tttggcggag gttcaggctt tggtaacacc gaccatgttt ggaaatgcca agcgtactac
     5641 gacaggactt gattttaacc gtgctagctt gattatggct gttttggaaa aacgggcagg
     5701 gcttctcttg caaaatcagg atgcctatct caaatctgct ggtggtgtta aattggatga
     5761 acctgcgatt gacttggctg ttgcagttgc tattgcttcg agctacaaag acaagccaac
     5821 taatcctcag gaatgttttg tcggagaact gggcttgaca ggagagattc ggcgcgtgaa
     5881 tcgtattgag caacgcatca acgaagctgc taaactgggc tttactaaga tttatgtacc
     5941 taagaattcc ttgacaggaa tcactctgcc taaggaaatt caggtcattg gcgtgacaac
     6001 gattcaggaa gtcttgaaaa aggtctttgc ataatccgtg acaaattctc ttaaaaatga
     6061 taagatagga gaaatatttg actatcaaat tttcaaggag ggaatcgtgt cgtattttga
     6121 acagtttatg caagccaatc aggcttatgt tgccctacat gggcagttaa atctgccact
     6181 taaacctaaa accagagtag ccattgtgac ctgtatggac tcacgtttgc acgttgcgca
     6241 agctctaggt ttggcacttg gggatgctca tattttgcgg aatgcaggtg gtcgagtgac
     6301 tgaggatatg attcgttctc tagttatttc ccagcaacaa atggggacaa gagaaattgt
     6361 ggtattgcat catacagact gtggtgctca gacctttgaa aatgaacctt ttcaggagta
     6421 tttgaaggaa gaattgggtg tcgatgtgtc agaccaggac ttcttgccct tccaagatat
     6481 agaagagagt gtacgcgagg atatgcaact gcttatcgag tctcccctaa taccagacga
     6541 tgtcattatc tctggtgcca tttacaatgt tgatacagga agtatgacag tcgtagaatt
     6601 ataaatactt catttagaaa gaaagtgtat gaagaaaagc aatattttat ttatttttat
     6661 tttattgcta tgtattggtt tacagtatga aaccatctac tatacggacg gttcgaggtc
     6721 aggtgcggaa tatggactaa tgggagtttc tatctttcta gctctctttt acatgattcc
     6781 ggctctttat tttctcttcc gtattgggaa aaatgggaat tgccaaagaa ggttttgatt
     6841 ctgtctttat tgggagggat gttcctttca ggctggttgt ctagttttgc taatacttat
     6901 atccatgatt tactgggggt tcttttccca gatagtccat ttttaaatgc ctttgaaagt
     6961 gctattgcgg ctcctttggt agaagaaccc ttgaaattat tgtcacttgt ttttgttttg
     7021 gctttgattc ctgtgcgaaa attaaaatct ttgtttttac ttggaattgc ttccggtttg
     7081 ggattccaaa tgattaagga tattggttat attcgtacgg atttgccaga gggctttgac
     7141 tttactattt cgcgaatttt agagcgtatc atctcaggaa ttgcctctca ctggactttt
     7201 tcaggtctag ctgtagtagg tgtttacttg ctttacagag cctataaagg acagaaggtt
     7261 ggcaagaaac agggccttat ttttctaggt ttagccttgg gaactcactt cttgtttaac
     7321 tctccttttg tggagttgga aacagagttg cctttagcga ttccagtggt tacggctatt
     7381 gctctctatg gtttttatca tgcttattgc tttgttgaga aacacaatga gttgatgacc
     7441 tagaatattt ttcaaaagaa tgatgcaagg gtacaaatat ggtgcccttc ttttattttt
     7501 gattgaaaaa tagtgcaaaa agcgctacaa tggtagatgg aaaatcttgt gaaaagcaca
     7561 agttatacat atataccgga ggaaatcatg tctttttctg atttaaagct gtttgccctt
     7621 tcttctaata aagaattggc agaacgtgtg gcgcaggaga ttgggataga gttggggaaa
     7681 tcaagtgttc gccaattttc agatggagag attcaggtca acattgaaga atcaatccgt
     7741 gggaaacacg tctttatctt acaatcaact agttcgcctg taaatgacaa tctgcttgaa
     7801 attttgatta tggtagatgc tttgaagcgt gcgagtgcag aatctgtcaa tgttgtcatg
     7861 ccttactatg ggtatgcacg tcaggataga aaggcgagag cgcgtgagcc aatcacttca
     7921 aaacttgtcg caaatatgct tgaagtagct ggagtggatc gtttattgac catcgacttg
     7981 catgctgcgc aaattcaagg attctttgat attcctgtgg atcatttgat gggtgctcct
     8041 ctgattgcag attattttga gcgtcgtggt atggttggtt ctgactatgt ggttgtcagc
     8101 ccggaccatg gaggggtgac tcgtgctcgt aagttggcag aatttttgaa aacatctatc
     8161 gctattattg ataaacgtcg tagcgttgat aagatgaata ctagtgaagt tatgaatatc
     8221 atcggtaagg ttgaaggcaa gacttgtatc ttgattgatg atatgattga taccgctgga
     8281 acgatttgtc atgcggcaga tgctcttgcg gaagctggtg ctgttgaagt ctatgcaagc
     8341 tgtacgcacc cagttctttc tggtcctgct acggacaata tccaaaaatc agctattaag
     8401 aaattggttg ttttggatac catctatctg ccagaagagc gtttgattga taagattgag
     8461 cagatttcaa tcgctcatct cctaggggat gctatcgtac gtattcatga aaaacgacca
     8521 ctttctccac ttttcgatat tgagaaaaaa atttaatgac caagcctgag atgattctca
     8581 ggttttttcg tcttttttcc gaataaatag atagtagcgg tgaatctagt aaagctagat
     8641 ttaaaactgt gatataataa aaagagaaga aatatgactg tacgtcatca gaagtttcag
     8701 cgaccatcat ttttgaacag tgatagcact tgaatcgacg ctttctaagg agaattcttg
     8761 taggcatacc agtcgtttca agataaggaa ttttagaagg tttttgaaag tcatatttct
     8821 tcaattggtt tccgcactca gggcaagatg gggcgtcgta gtccagtttg gcgatgattt
     8881 ccttgtgtgt atccttattg atgatgttta aaatctggac attagggtct ttaatgtcta
     8941 gtaattttgt gataaaatgt aattgttcca tatgattctt tctaatgagt tgttttgtcg
     9001 cttttcatta taggtcatat gggacttttt ttctacacaa aataggctcc ataatatcca
     9061 taggggattt acccactatg gatattatgg agccatttta caacagtgcc tcaaattcag
     9121 atactgacat acctaattgc tggctggcaa cctcagaagt cagaagacct tggcgtacta
     9181 tgtctaggaa ggcaaaaagt ttcctttctg cccttccttc aactttccca cgctctagtc
     9241 cttgttcaag gccttcagcc ctagcagttt ccaaggcata gtcctgtgct aacaagactt
     9301 gttcttctcg catatgtagt tgactaaaca ttttcctgtc ctcctcggac cagctcttgt
     9361 agtccagcag ttgatttgct tgggtaatgg ctcgttgcgg ttgctgggta aagggcttgt
     9421 tgccgaaaaa ctccaaccat ggcttgcgaa ccttgtcttt gctggtttct ctgtattttt
     9481 taattccaag aatgccatct taaccagatg gttttcctgt ccattgttgg taatcgccaa
     9541 tacctcacct gttgtgtctt cgcgcatact aaagctatga aaagccaggt catctgagaa
     9601 ataattacta tccacgatag caatggcgta aacaggagcg atgtgtttgt agctctagtg
     9661 agtatcacct tctcgctgac gaattttttc aagattttga ttaacctgac tgcacaggta
     9721 agcccacaag tgattgatga aaaaattctg atgatggact tgaatctcaa taattacttg
     9781 agtaccgtta tccaactccg ccaagacgtc tatactggta taaaaatcct gcaccgagta
     9841 aggcatggag agtaatacgt gaatatcgct tccctccaaa atggtcacat tttttgctgg
     9901 caagtccagc atatcgcgaa taaattgaca agtgatttct ggattgctga atattttctt
     9961 agcaatcaaa tcattagttg ggctgatgcc cggatgtctg agaatcctcc tattcctcct
    10021 tctatagtga gttgaaataa gatgtgaata actctatcag gaaagtcaaa ttaatttata
    10081 gaaatatttt agcagtcaag gtgtactatt atagattcaa tatactatat tatagcacat
    10141 ttttaaatct aggtttacta aattctctgg ctctatctat ttattcggaa aaagtttgaa
    10201 aaatacttgc tctagctctt cccaatggta ttttttgatt ctttccttta taatgggtgt
    10261 atggataaga aaaaattatt attgattgat gggtcttctg tagcttttcg ggcgtttttt
    10321 gcgctgtatc agcagttgga ccgttttaag aatgtggctg gtttgcatac caatgcgatt
    10381 tatggttttc agttgatgtt gagtcattta ttggagcggg ttgagccgag tcatattttg
    10441 gtggcttttg atgcgggaaa gacgaccttc cggacagaga tgtatgcgga ctataagggt
    10501 ggtcgggcca agactcctga tgagtttcgt gagcaatttc ctttcattcg tgagttgctg
    10561 gatcatatgg ggattcgtca ctatgatctg gctcagtatg aggcggatga catcattggg
    10621 acgctggata agctagcaga gcaggatggt tttgatatta ctattgtcag tggggacaag
    10681 gatttgattc agctgacgga tgagcatacg gtggttgaaa tttccaagaa aggtgtggct
    10741 gagtttgagg cctttacgcc agattacctc atggaagaaa tgggcctcac accagctcag
    10801 tttatcgatc tcaaggcgct catgggtgat aagtcggata atatccctgg ggtgaccaaa
    10861 gtcggtgaaa agacgggtat taagctcttg ctggagcatg gttcgcttga ggggatttat
    10921 gaaaatattg atggaatgaa gacttctaag atgaaggaaa atctcatcaa tgacaaggaa
    10981 caggcctttt tgtctaaaac actagcgacc attgatacca aggcaccgat tgcgattggt
    11041 ttagaggact tggtctatag tggtccagat gttgaaaatc ttgggaaatt ctacgatgag
    11101 atgggcttca aacagctaaa gcaggcttta aatgtgtcgt cagctgatgt gtctgagagt
    11161 ttggatttta ctattgttga ccaaatcagt caagatatgc tgagtgaaga gtctatcttc
    11221 cactttgagc tttttggtga gaattaccat acggataatt tggttggatt tgtctggtct
    11281 tgtggggata agctctatgc cacagacaag cttgagctgt tgcaagaccc gattttcaag
    11341 gatttcttag aaaaaacatc tctgagagtt tatgacttta agaaggttaa agttcttttg
    11401 caacgttttg gtgtggattt gcaggcgcct gcttttgaca tccgtttggc taaatacctc
    11461 ctttcgactg tggaggacaa tgaaattgcg accatcgcta gtctttatgg tcagacttac
    11521 ttggttgatg atgaaacttt ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag
    11581 aaattcttgg aacacttagc ttgtaaactt gctgttttgg tagaaacaga gcctatttta
    11641 cttgaaaaac tcagcgaaaa tgggcaatta gagcttcttt atgatatgga gcaacctctg
    11701 gcttttgtcc ttgccaagat ggaaattgct gggattatgg tcaagaaaga gaccttgctt
    11761 gagatgcagg ctgaaaatga gcttgtcatt gaaaaactga ctcaagagat ttacgagctg
    11821 gctggtgagg agtttaatgt caactcgcct aagcagttgg gcgtgcttct ctttgagaaa
    11881 ttgggacttc ctctagaata cactaagaaa accaagacag gttattcgac agcagtggat
    11941 gttttagagc gtctcgctcc tattgctccg attgttaaga aaatcctgga ttaccgtcaa
    12001 attgctaaga ttcaatctac ttatgtaatt ggcttgcagg actggatttt ggctgatgga
    12061 aagattcata ctcgctatgt gcaggatttg acccagaccg ggcgtttgtc tagtgtggat
    12121 ccaaacttgc aaaatattcc tgcccgattg gaacaggggc gcttgattcg gaaggctttt
    12181 gtgccagagt gggaggatag tgtgctactc agctctgact attcacagat tgaattgcgc
    12241 gttttggcgc atatttctaa ggatgagcac ttgattaagg ccttccaaga gggggcagat
    12301 atccatactt cgacagccat gcgggtcttt ggcattgagc gtcctgatga tgtgactgca
    12361 aacgaccgtc gcaatgccaa ggcagttaac tttggagtgg tttatgggat ttcagacttt
    12421 ggcttgtcta ataatttggg aattagtcgt aaggaagcca aagcctacat tgatacctac
    12481 tttgaacgtt ttccaggtat taaaaactac atggatgaag tggtgcggga ggcgcgtgat
    12541 aagggctatg tagagaccct ctttaagcgt cgccgtgagt tgccagatat caattcgcgc
    12601 aacttcaata ttcgtggttt tgcggagcga actgctatca actcacctat ccagggttcg
    12661 gcagcagata ttctcaagat tgccatgatt cagctggata aagccttggt tgcaggtggt
    12721 tatcagacta agatgctgtt acaagtgcac gatgaaatcg tccttgaagt gcctaaatct
    12781 gaattggtag agatgaaaaa attggtgaaa caaaccatgg aagaagccat tcaactcagt
    12841 gttcctctta tcgcagatga gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg
    12901 ggctagtcct ccttttttgt agtataattc tgcaaacctt ttcaaaatgt gctatactga
    12961 tgaaaaagga ggatttctat gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt
    13021 cagtatctcg caacgagtaa aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca
    13081 accagcaatc gagtaacaaa ggaaatgcag gctcggggct ataaaatcat cccagtcaat
    13141 cccaaggcgg caggtggcga aatcttgggt gaaaaggctt atgctagcct cgctgaaatt
    13201 ccttttcctg tagatattgt caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg
    13261 cgtgattttc tcaaagctga tgccaagatt ttttgggcac agctaggact tgaaagttta
    13321 gaagcgaaag agattttgcg tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc
    13381 aagagagaac atactcgctt gattgaggaa gcataagaaa aaggtagctg atgggctacc
    13441 ttttgtgtta tactcaatga aaatcaaaga gcaaactagg aaactagcca cgggctgctc
    13501 aaaacactgt tttgaggttg cagatagaac tgacgaagtc agctcaaaac actgttttga
    13561 ggttgtagat acgactgacg aagtcagctc aaacaccgtt ttgaggttgc agatagaact
    13621 gacgaagtca gtaacatata cctacggcaa ggcgacgttg acgtggtttg aagagatttt
    13681 cgaagagtat tagaaaatgc cgataagggt ctgcatacca agagtggtga ggatgatggc
    13741 aatccagcag atggctccaa gaagaatgga ttttccactg gatttgacca tagcgaccag
    13801 attggttttg agaccgatag cactcatggc catgacaata aggaatttag agagttcttt
    13861 gagaggagta aagaaactac tggacacacc tagagaggtg agtagtgtag tgaggagaga
    13921 ggcaaggata aagtaaagga taaaaagtgg gaagactttt ttcagttgca ggctttgctt
    13981 gttttcttgt tggcgacttt gccagtagga tagaaagagc gtgataggga taatggccaa
    14041 agtacgtgtg agtttaacaa tggttgcaga ctcgagggta ttgctttggt aaagactgtc
    14101 ccaagcgctg gcggcagccg ttacagagga agtgtcgttg accgcagtcc ctgcaaagag
    14161 ggcgaagcct tcattggata gatgaagcca ggtgccgagg gttggaaaga tgagcgcagc
    14221 caagacattg aagaaaaaga taacggaaat ggcttgggct acttcctttt ccttagcatc
    14281 aataacgggc gctgtcgctg caatggcaga acccccacag atagaagaac ctactccaac
    14341 caaggtagcc agttttgtat ccagggcaaa gaaacgctgg aagaggtagg caataatcag
    14401 agctattgag atagtggaca ggatgacagg gagtgaagat tggccaactg cgaagacctg
    14461 cgagatattg agaccaaaac caagcaagac aacggcatat tggagcaact tcttggaact
    14521 aaaggtcaaa ccagcatcca gttgtttata ggacgagaga aagggatgta ggagcatgcc
    14581 tatgaaaatg gcaaaaacgg gcgcgccaac cacagggaag aatcctccta agtaccaaga
    14641 taggatggaa atgagaaggc aggccaagat acctgctcca ttttttgata gaaatgacat
    14701 aaaaacctcc aaataaaatc tattaccatt atagacctat aaacaggaaa agtaaaatag
    14761 aaagtggaaa gctattctct aatgtatttt tgcggtcggg gggcttttgt agtataatag
    14821 agatacgttt tgaaagtagg aggtatctat ggacttaact aagcgcttta ataaacagtt
    14881 agataaaatt caagtttcgt tgattcgtca gtttgaccag gctatttcgg agattcctgg
    14941 ggtcttgcgt ttgaccttgg gggaacctga ttttacaacg ccagaccatg tcaaggaggc
    15001 gggcaagcga gcgattgatc agaaccaatc ctactataca gggatgagtg gtctgctgac
    15061 tctacgtcag gcagccagtg actttgttaa ggaaaagtac caactggact atgctcctga
    15121 aaatgaaatc ttggttacaa ttggggcgac agaggcttta tctgcgactt tgacggctat
    15181 tttggaagag ggagacaagg tacttttgcc agctcctgct tatccaggct atgaaccgat
    15241 tgttaactta gttggggcag aaattgttga gattgatacg actgaaaatg gttttgtctt
    15301 gactcctgag atgttggaga aggccatttt ggagcagggt gataagctca aggcggttat
    15361 tctcaactat ccagccaatc cgacaggaat tacctacagt cgagagcagt tagaggcctt
    15421 ggcagctgtt ttacgcaagt acgaaatttt tgttgtctgt gatgaggttt actcagaatt
    15481 gacctacaca ggcgaagccc atgtgtctct aggaacgatg ttgagagacc aggctattat
    15541 tatcaatggt ttgtctaaat cgcatgccat gacaggttgg cgtttggggc tgattttcgc
    15601 tcctgcgacc ttcacagccc agttaatcaa gagtcaccag tacttggtca ctgccgcaaa
    15661 taccatggcg caacatgctg cggtagaagc cttgacggct ggtaaaaacg atgcggagcc
    15721 catgaagaag gaatatatcc aacgtcggga ctatatcatc gaaaaaatga ctgctcttgg
    15781 ttttgagatt atcaaaccag acggtgcctt ctatattttt gctaaaattc cagcgggcta
    15841 caatcaagac tcctttgctt ttctgaagga ttttgctcag aagaaggccg ttgcctttat
    15901 ccctggtgca gcctttggac gttacgggga aggctacgtc cgcctatctt atgcagccag
    15961 catggagact atcaaagaag ccatgaaacg acttgaggag tacatgagag aagcatgatt
    16021 cagtctatca cgagtcaagg cttggtgctt tacaatcgca attttcgtga ggatgacaag
    16081 ctcgtcaaaa tttttacaga gcaggttggc aaacgcatgt tttttgtcaa acacgctggt
    16141 cagtctaagc tggcgcctgt tattcagccc ttggtgctgg cacgatttct cttgcgaatc
    16201 aatgatgacg gactcagtta catcgaagac tatcatgagg tcatgacttt tcccaagatt
    16261 aatagtgacc tctttgtcat ggcctatgcg acctatgtgg cagctcttgc agatgctagt
    16321 ttgcaggaca atcagcagga tgctcccttg tttgcttttt tgcaaaagac tttggagttg
    16381 atggaagcag gcttggatta tcaggttttg accaatattt ttgaaattca aattttgact
    16441 cgatttggaa tcagcctcaa ttttaatgag tgtgtcttct gccatcgggt tggtcaggct
    16501 tttgactttt ctttcaaata tggagcctgc ctctgtccag agcattatca tgaggataag
    16561 agacgttgtc atctcaatcc caatatcccc tatctgctca atcaatttca agctattgat
    16621 tttgagactt tggagaccat ttcgctcaag cctggaatca agcaagagct acgccaattt
    16681 atggatcaat tatatgaaga gtacgttggg attcacctaa aatcaaagaa atttattgat
    16741 tccctagcag actggggaca attactaaaa gaggaaaaga aatgaaaaaa atcgcagtag
    16801 atgccatggg gggcgattac gcacctcagg ccattgttga gggtgtcaat caagccctat
    16861 ctgacttttc agatatcgag gttcaacttt acggagatga agctaaaatc aagcaatatc
    16921 tgacagcgac agagcgcgtc agcattatcc atacggatga gaagattgat tcggatgatg
    16981 aacctacgag agctattcgg aataagaaaa atgccagtat ggtattggca gccaaggctg
    17041 tcaaagatgg tgaagcagac gctgtccttt cggctgggaa tacaggtgcc ttgttggcag
    17101 caggattctt catcgtgggt cgtatcaaga atatcgaccg tcctggactc atgtctacct
    17161 tgcctaccgt tgatggaaaa ggttttgaca tgctagacct tggtgccaat gcagaaaata
    17221 cagcccagca cctccatcaa tatgcggttc taggttcctt ctatgctaaa aatgtccgtg
    17281 gcattgcgca accacgcgtt ggtttgctca acaacggaac agagagtagc aagggcgacc
    17341 cgcttcgtaa ggaaacttat gaattactgg cggctgatga aagtttgaac tttatcggaa
    17401 acgtggaagc gcgtgatttg atgaatggcg ttgcagatgt tgttgtggca gatggtttca
    17461 cgggaaacgc tgtgctcaaa tccatcgaag ggacagctat gggaatcatg ggcttgctca
    17521 agacagctat tacaggtggt ggtcttcgag cgaaactagg tgccctcctt ctcaaggaca
    17581 gcctcagtgg tttgaaaaaa cagctcaatt attcagatgt tggtggagcg gtcttgtttg
    17641 gtgttaaggc acctgttgtc aagactcatg gctcaagcga tgccaaggct gtttatagta
    17701 cgattcgtca gatccgtacc atgctagaaa cagacgtggt tgcccagact gcgcgtgaat
    17761 tttcaggaga ataaaagaga tgacagaaaa agaaattttt gaccgtattg tgaccattat
    17821 ccaagagcga cagggagagg actttgtcgt gacagaatcc ttgagtctga aagacgattt
    17881 ggatgcggat tctgttgact tgatggagtt tatcttgact ctggaagatg aatttagtat
    17941 cgaaatcagc gatgaagaaa ttgaccaact ccaaaacgta ggagatgtgg ttaaaatcat
    18001 tcaaggaaaa tagcaatcgg agttccaagt caacggaagt agatggtttt tagaaatgag
    18061 aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
    18121 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccgctaa cagttgaaga
    18181 taaagcctgt aatcatgcgc tatctaagga gataagcaag gttgagaata tctttgccaa
    18241 agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac gcttcggatt
    18301 acgaatgaat ttgattgctg gtattatcaa tcatgaacta ggattctagt tttgcaggaa
    18361 gtctaatagt aaaaaagtga ttagaaaaca tcttttttaa aaatagagat gattttgaaa
    18421 caaaaaagct aattcaagac gtttcgatgc caattcaaga tttggatgaa aaaaattaat
    18481 agatactgtt atactaaact tgtcaagttt gtaacaagac aaatattaaa aataaaaaag
    18541 aggtattcgt tatgaataca aaaacgatgt cacaatttga aattatggat actgagatgc
    18601 ttgcttgcgt tgaaggtggc ggatgcaatt ggggagattt tgccaaagca ggtgttggag
    18661 gaggagcagc acgaggtctt cagctaggaa ttaaaacaag aacatggcaa ggtgcagcaa
    18721 ctggtgctgt gggaggagct atacttggag gtgtggccta tgcagcgaca tgttggtggt
    18781 aattatggat tttaaaagtt ttattattgg tttagtagtt ggtatatttg gtccttatat
    18841 ggatgattta attagaaaaa aatttttaaa gtcttcggag aagaaaacag aaaaatctgt
    18901 taaaaaataa tcaaaactat aaatgatgaa tctgaatcaa aattattttg cgcatgtaaa
    18961 gaggagtctt atagtaacga gtcaaaaaag gagtaactat gaatcgtaat ttagaacggt
    19021 gttatctatt ctgactagga atagatcata ccagaggtag cttagaaata gcagagacat
    19081 tagaaattga agtaataaat aggatgtcgt aagtgttact atcaatgatt tatttgtttc
    19141 aagcttgcct agggtgacag taaaaaatca atttcctttc aatagcatat ttttagtggg
    19201 caggactctt gttctgccta tttttttatc caaaaagtgc agttgggagg gagataggct
    19261 catttgggaa ggaagtccag tttttgttta gtgattgggg taagatagtt gttatcagat
    19321 gagttaatac tcttcgaaaa tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat
    19381 gtgactgact tcgtcagtcc tatctacaac ctcaaaacag tgttttgagc agcctacggc
    19441 tagtttccta gtttgctctt tgattttcat tgagtattag ggaaaaggag atgaatatga
    19501 aatttgggaa acgtcattat cgtccgcagg tggatcagat ggactgcggt gtagcttcat
    19561 tagccatggt ttttggctac tatggtagtt attatttttt ggctcacttg cgagaattgg
    19621 ctaagacgac catggatggg acgacggctt tgggcttggt caaggtggca gaggagattg
    19681 gttttgagac gcgagccatt aaggcagata tgacgctttt tgacttgccg gatttaactt
    19741 ttccttttgt tgcccatgtg cttaaggaag ggaaattgct ccactactat gtggtgactg
    19801 ggcaggataa ggatagcatt catattgccg atccagatcc cggggtgaag ttgactaaac
    19861 tgccacgtga gcgttttgag gaagaatgga caggagtgac tctttttatg gcacctagtc
    19921 cagactataa gcctcataag gaacaaaaaa atggtctgct ctcttttatc cctatattag
    19981 tgaagcagcg tggcttgatt gccaatatcg ttttggcaac actcttggta accgtgatta
    20041 acattgtggg ttcttattat ctgcagtcta tcattgatac ctatgtgcca gatcagatgc
    20101 gttcgacact agggattatt tctattgggc tagtcatcgt ctacatcttc cagcaaatct
    20161 tgtcttacgc tcaggagtat ctcttgcttg ttttggggca acgcttgtcg attgacgtga
    20221 ttttgtccta tatcaagcat gtttttcacc tccctatgtc cttctttgcg acacgcagga
    20281 caggggagat cgtgtctcgt tttacagatg ctaacagtat catcgatgcg ctggcttcga
    20341 ccatcctttc gattttccta gatgtgtcaa cggttgtcat tatttccctt gttctatttt
    20401 cacaaaatac caatctcttt ttcatgactt tattggcgct tcctatctac acagtgatta
    20461 tctttgcctt tatgaagccg tttgaaaaga tgaatcggga taccatggaa gccaatgcgg
    20521 ttctgtcttc ttctatcatt gaggacatca acggtattga gactatcaag tccttgacca
    20581 gtgaaagtca gcgttaccaa aaaattgaca aggaatttgt ggattatctg aagaaatcct
    20641 ttacctatag tcgagcagag agtcagcaaa aggctctgaa aaaggttgcc catctcttgc
    20701 ttaatgtcgg cattctctgg atgggggctg ttctggtcat ggatggcaag atgagtttgg
    20761 ggcagttgat tacctataat accttgctgg tttactttac taatcctttg gaaaatatca
    20821 tcaatctgca aaccaagctt cagacagcgc aggttgccaa taaccgtcta aatgaagtgt
    20881 atctagtagc ttctgagttt gaggagaaga aaacagttga ggatttgagc ttgatgaagg
    20941 gagatatgac cttcaagcag gttcattaca agtatggcta tggtcgagat gtcttatcgg
    21001 atatcaattt aaccgttccc caagggtcta aggtggcttt tgtggggatt tcagggtcag
    21061 gtaagacgac tttggccaag atgatggtta atttttacga cccaagtcaa ggggagatta
    21121 gtctgggtag tgtcaatctc aatcagattg ataaaaaagc cctgcgccag tacatcaact
    21181 atctgtctca acagccctat gtctttaacg gaacgatttt ggagaatctt cttttgggag
    21241 ccaaggaggg gacgacacag gaagatatct tacgggcggt cgaattggca gagattcgag
    21301 aggatatcga gcgcatgcca ctgaattacc agacagaatt gacttcggat ggggcaggga
    21361 tttcaggtgg tcaacgtcag agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg
    21421 tcttgatttt ggatgaggcg actagcagtt tggatatttt gacagagaag cggattgtcg
    21481 ataatctcat tgctttggac aagaccttga ttttcattgc tcaccgcttg actattgctg
    21541 agcggacaga gaaggtagtt gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg
    21601 ctgatttgct tgcacagggt ggcttttacg cccatttggt caatagctag aaagaggaga
    21661 ggatgaaacc agaattttta gaaagtgcgg agttttataa tcgtcgttac cataattttt
    21721 ccagtagtgt gattgtaccc atggcccttc tgcttgtgtt tttacttggc tttgcaactg
    21781 ttgcagagaa ggagatgagt ttgtccacta gagctactgt cgaacctagt cgtatccttg
    21841 caaatatcca gtcaactagc aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc
    21901 tggttaagaa gggggatctt ttggttcaat accaagaagg ggcagagggt gtccaagcgg
    21961 agtcctatgc cagtcagttg gacatgctaa aggatcaaaa aaagcaattg gagtatctgc
    22021 aaaagagcct gcaagaaggg gagaaccact ttccagagga ggataagttt ggctaccaag
    22081 ccacctttcg cgactacatc agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa
    22141 atgagaccat cgcgtcccag aatgcagcag ctagccaaac ccaagccgaa atcggcaacc
    22201 tcatcagtca aacagaggct aaaattcgcg attaccagac agctaagtca gctattgaaa
    22261 caggtgcttc cttggccggt cagaatctag cctactctct ttaccagtcc tacaagtctc
    22321 agggcgagga aaatccccaa actaaggttc aggcagttgc acaggttgaa gcacagattt
    22381 ctcagttaga atctagtctt gctacttacc gtgtccagta tgcaggttca ggtacccagc
    22441 aagcctatgc gtcagggtta agcagtcaat tggaatccct taaatcccaa cacttggcaa
    22501 aggttggtca ggaattgacc cttctagccc agaaaatttt ggaggcagag tcaggtaaga
    22561 aggtacaggg aaatctttta gacaagggga aagttacggc gagtgaggat ggggtgcttc
    22621 atcttaatcc tgagaccagt gattctagca tggttgcaga aggtgcccta ctagcccaac
    22681 tttatccatc tttggaaaga gaagggaaag ccaaactcac agcttatcta agttcaaaat
    22741 atgtagcaag aatcaaggtc ggtgattctg ttcgctatac tacgactcat gatgccggga
    22801 atcaactttt cctagattct actattacaa gtattgatgc gacagctact aagactgaga
    22861 aagggaattt ctttaaaatc gaggcggaga ctaatctaac ttcggagcag gctgaaaaac
    22921 ttaggtacgg ggtggaaggc cgcttgcaga tgattacggg caagaaaagt tacctacgtt
    22981 attatttgga tcaatttttg aacaaagagt aatgttcgtg tttttagagt taaataattt
    23041 ttaaactgtg agaaagattc ttcttgcagt tttttcttta caatttttga aaaatatcta
    23101 ctatttattc ggttaaattc ttgtgttttt tggttttttg tggtaaaatg tgctcaagta
    23161 atacgaaagg cgaactttaa aatgtcaaaa caattgatct attcgggaaa agctaaagat
    23221 atctatacaa ctgaggatga aaatcttatt atttcaactt acaaggacca ggcgactgct
    23281 ttcaacggtg tcaagaagga gcagattgca ggtaagggag tcttgaataa tcagatctca
    23341 tcttttattt ttgagaaatt aaatgtggct ggtgtggcga ctcactttgt ggagaaactt
    23401 tcagacacgg aacaactcaa taaaaaggtt aagattattc ctttggaagt cgtgctccgc
    23461 aactatactg ctggttcctt ttcaaaacgt tttggtgtgg atgagggaat cgccttggag
    23521 actccgattg tcgaatttta ctacaaaaat gatgatttgg atgatccatt tatcaatgat
    23581 gagcatgtga aattcctaca gattgcgggt gaccagcaga ttgcctactt gaaggaagaa
    23641 acgcgtcgta tcaatgaact attgaaagtc tggtttgctg agattgggct taaattgatt
    23701 gactttaagc tagagttcgg ttttgacaag gatggcaaga ttatcttggc agacgaattt
    23761 tcaccagata actgccgctt gtgggacgct gatggcaacc acatggataa ggatgttttc
    23821 cgtagaggat tgggagaact aaccgacgtt tatgagattg tttgggaaaa gttgcaggaa
    23881 ttgaaataat ctgtttgcaa cggaaaacct tcgtctctca actaaaagga ctcaggctga
    23941 aaaggtcccc cagacctttt cactctgtag agaactaggt gaactaacag atgtttacga
    24001 aattgtctgg gaaaagttgc agggtttaaa ataacaacct caaggctgtt tgggaatatt
    24061 gcaagagctg aaataaagga ataagaattg atggataaac gtatttttgt tgaaaaaaag
    24121 gctgattttc aggtcaagtc agagagtttg gttagagagc tccagcacaa cttgggactg
    24181 tcaagcttga aaagtattcg tattgtgcaa gtatatgatg tatttgactt ggctgaggac
    24241 ttgtttgcac ctgcagagaa gcacattttc tctgagcagg taaccgacca tgttttagat
    24301 gaagtatctg tgcaggcgga tcttgctaac tatgctttct ttgccattga aagtctgcca
    24361 gggcagtttg accagcgtgc agcttcgtca caggaagcct tgcttttgtt gggaagttcg
    24421 agtgacgtga cagtcaacac agcccaactt tacttggtga ataaagatat tgatgcgact
    24481 gagttggaag ctgtcaaaaa ctacctgctc aatccagttg attctcgttt caaggatatc
    24541 acgacaggga ttgccaagca ggagttttca gagtcagaca agaccattcc caaattgact
    24601 ttctttgaaa gctatgcagc agaagacttt gctcgctaca aggccgaaca agggatggcc
    24661 atggaagtgg atgatttgct ctttatccaa gactacttta agtcaatcgg gcgcgtgcca
    24721 actgagactg aactcaaggt tttggacact tactggtctg accactgccg tcatacgact
    24781 tttgagacag agttgaaaca catcgacttt tcagcttcta aatttcaaaa gcaattgcag
    24841 tcaacctatg acaagtatat tgccatgcgc gaggaattag gtcggtctga aaaaccacaa
    24901 accttgatgg atatggcgac tattttcggt cgttatgagc gtgctaatgg acgattggat
    24961 gatatggaag tctctgacga aatcaatgcc tgctcagttg aaattgaagt ggacgttgat
    25021 ggtgtcaagg aaccttggct cctcatgttt aaaaacgaaa cccacaacca tccaacagaa
    25081 attgagccat ttggtggagc ggctacctgt attggtggag ctattcgtga tccgttgtca
    25141 ggccgttcct atgtttacca agccatgcgt atttcaggtg ctggtgatat tacagcaccg
    25201 atttcggaaa ctcgcgctgg gaaattgcca caacaagtca tttctaaaac agcagctcat
    25261 ggttattctt catatggtaa ccagattggg cttgcaacaa cctacgttcg tgaatacttc
    25321 cacccaggct ttgtagctaa acgtatggaa cttggtgccg ttgttggtgc gactcccaag
    25381 ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga tcatccttct cggaggcaaa
    25441 acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta aggttcaaac agttgagtct
    25501 gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca tcgaagaacg caagattcag
    25561 cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga agtccaatga ctttggggca
    25621 ggcggtgtct gtgtggctat cggtgaattg gcagacggtc ttgaaatcga cctcaacaag
    25681 gtgcctctta aataccaggg cttgaatggt acagaaattg ccatctctga atcacaagaa
    25741 cggatggcgg tcgtggttcg tcctgaagat gtggatgcct tcgttgccga atgtaacaaa
    25801 gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa aaccaaatct tgtcatgcac
    25861 tggaatggtg agacaatcgt tgacttggag cgtcgtttcc ttgacaccaa tggtgtgcgc
    25921 gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac tcccagaaga gcgtcaaaca
    25981 tctgctgaaa cactggaatc agataccctt acggttctat ctgacctcaa ccatgcaagt
    26041 caaaaaggat tacagactat ctttgactgc tctgttggac gctcaacggt taatcaccca
    26101 cttggtggtc gttaccaact cacaccaact gaggcatctg tgcagaaatt gccagttcaa
    26161 cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt tcaacccata tgtagctgaa
    26221 tggtctccat accacggtgc tgcttatgcg gttatcgaag caactgctcg tttggtggct
    26281 gctggtgcca actggttcaa ggctcgtttc tcttaccaag agtatttcga gcgtatggat
    26341 aaacaagcag agcgtttcgg tcagccagta gctgctcttc taggttctat tgaagcacaa
    26401 attcagcttg gcttgccatc tatcggtggt aaggactcca tgtctggtac ctttgaagaa
    26461 ttgaccgttc cgccaacctt ggctgccttt ggggtgacga cggcagatag ccgtaaggtg
    26521 ctctctccag aatttaaagc tgttggggaa aatatctact acatcccagg tcaagccctc
    26581 tctgcagaga ttgattttga cttgattaag aaaaattttg ctcagtttga agccatccaa
    26641 gctgaccata aagtgacatc tgcatcagct gtcaaatacg gtggtgtagt tgaaagtttg
    26701 gctcttgcta cctttggaaa ctatattggt gcagaggtga ccttgcctga acttgaaaca
    26761 gctttgacag ctcaattagg cggctttgtc ttcacatctc ctgaagaaat tgctggagta
    26821 gagaaggttg gacaaacgaa agcagacttt acactgactg tcaacggtgt gaagctagat
    26881 ggacacaagc ttgacagtgc atttcaaggg acattggaag aagtttaccc aacagaattt
    26941 acccaagcga aagaactaga agaagtacca gctgtggcat cagatgttgt gattaaagcc
    27001 aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct ttccaggaac caactcagaa
    27061 tatgattcag ctaaggcctt cgaaaaagaa ggtgcagagg tcaatttggt gccattcgtg
    27121 accttgaatg aagaagctat tgtcaagtca gttgaaacta tggttgacaa tatcgacaag
    27181 actaatattc tcttctttgc tggtggattc tcggctgcgg atgaaccaga tggttcagct
    27241 aagtttatcg tcaatatcct gcttaatgaa aaagtgcgtg tggctattga tagctttatc
    27301 gcccgtggtg gtttgattat cggtatttgt aatggattcc aagccttagt caaatcgggt
    27361 ctcctaccct acggaaactt tgaagctgct aacagtacta gcccaaccct cttctacaat
    27421 gatgccaacc aacacgtggc caagatggtg gaaactcgca ttgccaatac caactcacca
    27481 tggttggttg gtgtgcaagt gggcgatatc cacgctattc ctgtttcgca cggtgaaggg
    27541 aagtttgtcg tgacggctga ggaatttgca gagctccgtg acaatggaca aattttcagc
    27601 caatacgttg actttaacgg taaaccaagt atggattcta agtacaatcc gaatggttct
    27661 gtccatgcca tcgaaggaat taccagcaag aatggtcaaa tcatcggtaa gatgggccac
    27721 tcagaacgtt atgaggatgg tcttttccaa aatatcccag gcaataaaga ccaacacctg
    27781 ttcgcatcag cggttaaaca tttcactgga aaataagact tacagatttt ctaatagata
    27841 gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat gattacaaat gaaaattagg
    27901 tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga atgtggtgtt ttcggtattt
    27961 ggggacatcc agatgctgct aagttgacct attttggact ccacagtctt caacaccgtg
    28021 gtcaggaggg ggcaggaatc ctctccaatg atcaaggaca actgaagcgc catcgtgaca
    28081 tggggctttt atcagaagtt ttcagaaatc cagctaattt ggataaattg acaggagctg
    28141 gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc ttctgtagat aacatccagc
    28201 ccttcctctt ccgttttcac gatatgcagt ttggtttggc tcataatgga aatctgacca
    28261 atgcagcctc tctcaagaaa gaactggaac aaagaggagc aattttcagc gcgacttcgg
    28321 actctgaaat cttggctcac ctcattcgtc gcagtcataa tcctagcctg atgggcaaaa
    28381 tcaaggaagc gctcagcctt gtcaaaggtg gttttgccta tatcttgctg tttgaggaca
    28441 agttgattgc ggctcttgac ccaaatggat tccgaccgct ttcgattggt aaaatggcta
    28501 atggagcagt tgttgtatct tctgaaacct gtgcttttga ggtcattggt gccgagtgga
    28561 ttcgtgattt gaagccaggt gagattgtga tcattgatga cgagggcatt cagtatgaca
    28621 gctatacaga tgatacccag ttggcggttt gttctatgga gtatatctac tttgctcgcc
    28681 ctgattctaa tatccacggt gtcaatgtcc atacggcacg taagagaatg ggagcgcaat
    28741 tggcgcgaga atttaagcat gaggcagata ttgtagttgg tgtgcccaat tcttccctaa
    28801 gcgcggctat gggatttgcg gaagaatcag gcttaccaaa tgaaatgggt ctgatcaaaa
    28861 accaatacac ccagcgaact tttatccaac cgactcaaga attgcgggag caaggagtgc
    28921 ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa acgtgtggtc atggtggatg
    28981 attccattgt acgtggaaca acctctcgtc gtatcgttca gctcttgaaa gaagcgggtg
    29041 cgactgaggt tcacgttgcc attggaagtc ctgcactagc gtatccatgt ttctacggga
    29101 ttgatatcca gacccgtcag gagctgattg cagccaatca tacggtcgaa gaaactcgcc
    29161 aaatcattgg tgcggacagt ctgacttatc tttcaattga tggcttgatt gagtcgattg
    29221 gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc ttactttgac ggtgactacc
    29281 caacgcctct ttatgactac gaagaagact atcgtagaag tttggaagaa aagaccagtt
    29341 tttacaagta ggcgacagat tctccattaa agaaaaggaa aaaataaatg acaaataaaa
    29401 atgcatatgc ctcacgtctc actactgact aaaggcttaa gcatttagtc agtagacgct
    29461 ttgtcctata ggatcaaagc tagagccctg actagtattt ttagataaaa agatggttta
    29521 tctaaaaata cgtcgcagtc tttctcaaaa aaagaaaagg aaaaataaaa tggcaaataa
    29581 aaatgcgtac gctcaatctg gtgtggatgt tgaagcgggt tatgaagttg ttgaacggat
    29641 taaaaagcac gtggcccgta cggagcgtgc aggtgtcatg ggagctcttg gtggctttgg
    29701 tggtatgttt gacctttcca agactggggt taaagaaccc gtcttgattt cagggactga
    29761 cggtgtcgga accaagctca tgttggctat caagtacgac aagcacgata ccatcgggca
    29821 ggactgtgtg gccatgtgtg tcaacgacat cattgctgca ggtgcggaac ccctctattt
    29881 tctcgactac gtagcgacag ggaagaatga accagctaag ctagaacaag tggttgctgg
    29941 tgtggcagaa ggttgtgtgc aggctggtgc tgccctcatc ggtggggaaa cggctgaaat
    30001 gccgggcatg tacggcgaag acgactatga cttggctggt tttgcggtcg gtgtggctga
    30061 aaaatctcaa atcattgacg gttcaaaggt ggtagaggga gatgttcttc tcggacttgc
    30121 ttcaagtggg attcactcaa atggttactc tttggttcgt cgtgtctttg cggattacac
    30181 aggtgaggaa gtcctaccag aattggaagg caagaaactt aaggaagttc tacttgagcc
    30241 gactcgtatc tatgtcaagg ctgtcttgcc gctcatcaaa gaagagttgg tcaacggcat
    30301 tgcccacatc acaggtggtg gctttatcga aaatgtccct cgtatgtttg cagatgacct
    30361 agctgctgaa attgatgaaa gtaaagttcc agtgcttcca attttcaaaa cccttgaaaa
    30421 atacggtcag attaaacacg aagaaatgtt tgaaatcttc aatatgggtg tgggacttat
    30481 gttggcggtc agccctgaaa atgtagagcg tgtaaaagaa ttgttggatg aagcagtcta
    30541 tgaaattggt cgcatcgtca agaaagaaaa cgaaagtgtc attatcaaat gaaaaaaata
    30601 gcggtttttg cctctggtaa tggctcaaat tttcaggtga ttgccgaaga atttccggtg
    30661 gagtttgtct tttcagacca tcgtgatgcc tatgtgcttg agcgtgcaaa gcagctcggc
    30721 gttctgtcct atgcttttga actcaaggag tttgagagca aggcagacta cgaagcagcc
    30781 cttgtcgaac tcttggaaga acaccagatt gacttggttt gcctagcagg ctacatgaaa
    30841 atcgttggac caaccttatt gtcggcttat gaaggtcgga ttgtcaacat tcatccagcc
    30901 tacttgccag aatttccagg agctcatggg attgaggatg cttggaatgc tggcgtgggt
    30961 cagtctggtg tgaccattca ctgggtggat tcgggtgtgg atacaggcca ggtcatcaaa
    31021 caggttcgtg tgccacgact agctgatgat accattgaca gatttgaagc tcgcatccat
    31081 gaagcagagt acaggctgta tccggaagta gtgaaggctc tatttacaga ttgacttttt
    31141 gatgattcat atgatatctt tgattttaaa ttggagtcag tgtttgttga agacggcttc
    31201 aaacggaggt atttgtaatg ttagaatcta aaaaaacaac tcgatatgta ttttatgtct
    31261 atctgatgtt attaacttgg ggaatcttat ttaagtttga aacaaatcct gaatttatag
    31321 catttttctt agctccaagg tatatcaatt ggattccatt ttcagaacca ctaatagtcg
    31381 atggaaaaat tgtttttgct gaaatgttat ttaatctgat tttctttatt ccattaggtg
    31441 tttgtttccc tttgataaaa actaatttat ctagtttaag aatagtcggg acaggtttct
    31501 tgattagttt attgtttgag tgcttacagt atattttagc aataggtata acagatataa
    31561 cggatttgac tttaaatacg ctaggtgtct gtgtaggctt actgatttat caaattttta
    31621 taagagtgtt caaatcacag actagaaaat ggatcaatat cttaggtatg cttagccttg
    31681 gttttgctta tcttgtttta ctgttactgc atttacttag tgtttaacta atgattaaaa
    31741 aggagaatat aatgactaaa cgcgtcttaa tcagcgtctc agacaaagcg ggcattgttg
    31801 aatttgccca agaactcaaa aaacttggtt gggagattat ctcaacaggt ggaactaagg
    31861 ttgcccttga taatgctggg gtggatacca ttgctatcga tgatgtgact ggtttcccag
    31921 aaatgatgga cggtcgtgtg aagaccctcc acccaaatat ccacggaggg cttctcgctc
    31981 gtcgtgactt ggatagccac ttggaagcgg ctaaggacaa caagattgag ctcattgacc
    32041 ttgtggtggt caacctttac ccatttaagg aaactatcct taaaccagat gtgacttatg
    32101 ctgatgcagt tgaaaatatc gatattggtg ggccatctat gcttcgttca gcagcgaaaa
    32161 atcatgccag tgttacagtt gtggtagatc ctgctgacta cgctgtggtt ttggatgaat
    32221 tggcagcaaa cggcgaaacc tcttatgaaa ctcgccaacg tttagcagcc aaagtatttc
    32281 gtcacacagc ggcttatgac gccttgattg cagaatactt cacagctcaa gtgggtgaaa
    32341 gcaagcctga aaaactcact ttgacttatg acctcaagca accaatgcgt tacggtgaga
    32401 atcctcaaca agacgcggac ttttaccaga aagctttgcc tacagactac tccattgctt
    32461 cagccaaaca gctcaacggg aaagaattgt catttaataa tatccgtgat gcagatgctg
    32521 ctatccgtat catccgtgac ttcaaagata gtccaaccgt tgtggctctc aaacacatga
    32581 atccatgtgg aattggtcaa gctgatgaca tcgagactgc ttgggactac gcttatgagt
    32641 ctgacccagt gtctatcttt ggtgggattg tcgtcctcaa ccgtgaggtg gatgctgcga
    32701 cagctgagaa gatgcacggc gttttcctcg aaatcatcat tgcaccaagc tatacggatg
    32761 aagcgctagc cattttgatc aataaaaaga aaaacttgcg tatccttgcc ttgccattta
    32821 atgctcaaga ggctagcgaa gtggaagcag aatacacagg tgtagtcggt ggacttctcg
    32881 tgcaaaatca agacgtggtc aaggaaagcc cagctgactg gcaagtggtg actaaacgtc
    32941 agccaactga gacagaagcg actgctcttg agttcgcttg gaaggctatc aagtacgtca
    33001 aatcaaatgg tattatcgtg accaacgacc acatgacact tggtgttggt ccaggtcaaa
    33061 ccaaccgtgt ggcttctgtt cgccttgcca ttgaccaagc caaagatcgt ctggacgggg
    33121 cggtccttgc ttcagatgcc ttcttcccat ttgcggataa cgtggaagaa atcgccaaag
    33181 caggaattaa ggccatcatc cagcccggtg gctctgtccg tgaccaagaa tccatcgaag
    33241 cagcggataa atacggcttg actatggtct ttacaggtgt gagacatttt agacattaag
    33301 aagataaaag ggaagaaaac agtttctttc cttttttggc ttaaaatact aactgaaaca
    33361 agattaaaac gaactttttt gatataatgt tggtaaataa ttcgcaaaag aggttgagga
    33421 atgaaactgc ttgttgtcgg ttctggtggt cgtgagcatg cgattgctaa aaagttactt
    33481 gaatcaaaag acgtggaaaa agtctttgta gctcctggga atgatgggat gactctggat
    33541 ggtttggaat tggtaaatat ctctatttcc gaacattata aattgattga cttcgcaaag
    33601 accaatgatg ttgcttggac ctttatcggt ccagatgatg cccttgctgc tggtatcgtg
    33661 gatgatttta accaagctgg acttaaggcc tttggtccga ctagggctgc agcggagctg
    33721 gagtggtcca aggatttcgc caaggaaatc atggtcaaat acggcgttcc gacagcaaca
    33781 tatggcacat tttcagattt cgaggaagcc aaagcctata tcgaaaagca tggtgcgcct
    33841 atcgtagtca aggcggatgg cttggcactt gggaagggtg tcgtcgttgc tgagacggtt
    33901 gagcaagcgg tcgaagccgc tcatgagatg cttttggaca ataaatttgg tgactcaggt
    33961 gcgcgcgtgg ttattgagga attccttgaa ggagaggaat tttcactctt tgcctttgtc
    34021 aatggtgata agttctacat catgccaacg gctcaggacc acaaacgtgc ctatgatggc
    34081 gacaaagggc ctaacacggg tggtatgggt gcctatgcgc cagtcccaca cttaccacag
    34141 agtgtagttg atacagcggt tgacaccatt gtcaagccag ttctagaagg ggtgattaaa
    34201 gaaggtcgcc cttatctggg agttctttac gcagggctta tcctgacagc tgatggaccg
    34261 aaagtcattg agttcaacgc tcggttcgga gatccagaaa ctcagattat cttgcctcgc
    34321 ttgacctctg actttgctca aaatatcaca gatatcctgg atagcaagga gccaaatatc
    34381 atgtggacgg acaagggtgt gactctgggt gtggttgtcg catccaaggg ctacccgcta
    34441 gactatgaaa ggggcgttga gttgccagcc aagacagaag gcgatgtcat cacctactat
    34501 gcaggggcta agtttgcgga aaatagcaga gcactgctct caaacggcgg acgagtttat
    34561 atgctcgtta ccacagcaga taccgtcaaa gaagcccaag ccagcatata ccaagaacta
    34621 taccaacaaa aaatagaagg actcttctac cgaacagata tcggaagcaa ggcaattaag
    34681 taaagatata agaataacgc gccgtagtcg ccaaacacga taatggtcgt cgtggtgaaa
    34741 agaccagaac agtgaatgtt ctggtcaggg ggaaacttgg agaccttagg ctcaaagttt
    34801 aggaatgaaa ccgaaggttt gcttccgcct ccatcaccta agaccattat caaaaagaaa
    34861 aataaaaatt cacaaaatac gttaatgatc gtatggtttg cgagcgttag cgagctaata
    34921 tagaacaatc accgccgttg tgaaagaacg attggatgat aatccaatcg ttcagggaaa
    34981 ttggaagacc ttgggtttcc aatttaggca tgagacacct ttggtggctg ctgccgtccc
    35041 tcacaagcta aggtgattgt tgaaaaagag gaaaaaggag aagaaatgaa accagtaatt
    35101 tccatcatca tgggctcaaa atccgactgg gcaaccatgc aaaaaacagc agaagtccta
    35161 gaccgcttcg gtgtagccta cgaaaagaaa gttgtttccg cacaccgtac accagacctc
    35221 atgttcaaac atgcagaaga agcccgtagt cgtggcatca agatcatcat cgcaggtgct
    35281 ggtggcgcag cgcatttgcc aggcatggta gctgccaaaa caacccttcc agtcattggt
    35341 gtgccagtca agtctcgtgc tcttagtgga gtggattcac tctattctat cgttcagatg
    35401 ccgggtgggg tgcctgttgc gaccatggct atcggtgaag ctggagcgac taacgcagct
    35461 ctctttgccc tccgtctcct ctctgtagaa gataagtcca ttgcggatgc acttgccaac
    35521 tttgctgaag aacaaggaaa aatcgcagag gagtcgtcaa atgagctcat ctaaaacaat
    35581 cggaattatc ggtggcggtc aactgggtca gatgatggcc atttctgcta tctacatggg
    35641 ccacaaggtt atcgcgctgg atcctgcggc ggattgcccg gtctctcgtg tggcggaaat
    35701 cattgtggca ccttataacg atgtagacgc cctccgtcag ttggcagacc gttgcgatgt
    35761 cctcacttat gagtttgaaa atgtcgacgc tgacggtttg gatgccgtta tcaaggatgg
    35821 acaactccct caaggaacag atctgctccg catttcgcaa aatcgtattt ttgaaaagga
    35881 ctttttgtca aacaaggctc aagtcactgt ggcaccctac aaggtcgtga cttctagcct
    35941 agacttggca gatatcgact tgtcgaaaaa ctatgtcctc aagactgcga ctggtggcta
    36001 cgatggtcat ggacaaaagg ttattcgttc agaagcagac ttggaagcag cctatgcgct
    36061 agcagactca gcagactgcg tcttggaaga atttgtcaac tttgaccttg agatttctgt
    36121 catcgtgtca ggaaatggca aggaggtgac gtttttccca gttcaggaaa atatccaccg
    36181 caacaatatc ctgtctaaga ccatcgtacc agcccgcatt tctgaaagtc tagtagacaa
    36241 ggctaaagct atggcagtgc gaatcgcaga acaactcaac ttgtctggaa ctctctgtgt
    36301 ggaaatgttt gcgacagctg atgacatcat tgtcaatgaa atcgccccac gaccacataa
    36361 ctctgggcac tattctattg aagcctgtga tttctctcag tttgacaccc atattctggg
    36421 tgttctcgga gcaccattac cagtcatcaa actccatgcg ccagccgtta tgcttaatgt
    36481 cctcggtcag catgtcgagg ctgctgaaaa atatgtcaca gaaaatccaa gcgcccacct
    36541 ccacatgtat ggtaaaatag aagcaaagca taatcgtaag atgggacatg tgactttgtt
    36601 tagtgatgtg ccggatagtg tggaagagtt tggggaaggg attgattttt aggacaagtc
    36661 tatgatacaa attatcgtta atacatttat tgaaaagtat aagactggag cagttgttga
    36721 agtgttgtat gccagtgctg accaagataa ggtacaagct aaatatgaag aactagctgc
    36781 acaatacccc gaaaattatt tagctatcta taatgtaccg ctggatacgg atttgaatac
    36841 actagatcat tacccgtctg tgtttattgg aaaagaggag tttgagtaga aatcttggtt
    36901 tacctagata gcttattccc aacagcttaa gaagaaagga aaaattaaca catgatcaac
    36961 cgttactctc gccctgagat ggcgaatatt tggagtgaag aaaataaata ccgtgcttgg
    37021 cttgaggtgg aaatcctctc tgacgaggca tgggctgagt tgggggaaat ccctaaggaa
    37081 gatgtggctt tgattcgcaa gaaggcggac tttgacatcg accgtatttt ggaaattgag
    37141 caggagacgc gccacgatgt ggtggctttc acgcgtgcgg tttctgagac tcttggtgaa
    37201 gagcgcaagt gggttcacta tgggttaact tctactgacg tggtggatac tgcttatggt
    37261 tacctctaca agcaggccaa cgacatcatc cgtcgtgacc ttgaaaactt cactaatatc
    37321 atcgctgaca
//
LOCUS       DBDESG010000015        35258 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.015, whole genome shotgun sequence.
ACCESSION   DBDESG010000015 DBDESG010000000
VERSION     DBDESG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.015"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(113..2218)
                     /locus_tag="ACKB38_000734"
     CDS             complement(113..2218)
                     /locus_tag="ACKB38_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGL7979539.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQGGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMEASDIERLKDMAHRLQDKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGNFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(2827..3807)
                     /gene="mraY"
                     /locus_tag="ACKB38_000735"
     CDS             complement(2827..3807)
                     /gene="mraY"
                     /locus_tag="ACKB38_000735"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGL7979540.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(3809..6061)
                     /gene="pbp2X"
                     /locus_tag="ACKB38_000736"
                     /gene_synonym="pbpX"
     CDS             complement(3809..6061)
                     /gene="pbp2X"
                     /locus_tag="ACKB38_000736"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGL7979541.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRTMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6065..6382)
                     /gene="ftsL"
                     /locus_tag="ACKB38_000737"
     CDS             complement(6065..6382)
                     /gene="ftsL"
                     /locus_tag="ACKB38_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGL7979542.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6394..7344)
                     /gene="rsmH"
                     /locus_tag="ACKB38_000738"
     CDS             complement(6394..7344)
                     /gene="rsmH"
                     /locus_tag="ACKB38_000738"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGL7979543.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQACLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            7510..7704
                     /locus_tag="ACKB38_000739"
     CDS             7510..7704
                     /locus_tag="ACKB38_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979544.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            7720..8262
                     /locus_tag="ACKB38_000740"
     CDS             7720..8262
                     /locus_tag="ACKB38_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HGL7979545.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            8273..8515
                     /locus_tag="ACKB38_000741"
     CDS             8273..8515
                     /locus_tag="ACKB38_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979546.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            8526..8861
                     /locus_tag="ACKB38_000742"
     CDS             8526..8861
                     /locus_tag="ACKB38_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979547.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(8971..9972)
                     /locus_tag="ACKB38_000743"
     CDS             complement(8971..9972)
                     /locus_tag="ACKB38_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7979548.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(10032..11933)
                     /locus_tag="ACKB38_000744"
     CDS             complement(10032..11933)
                     /locus_tag="ACKB38_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGL7979549.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNQEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEVLTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(11955..12248)
                     /gene="yajC"
                     /locus_tag="ACKB38_000745"
     CDS             complement(11955..12248)
                     /gene="yajC"
                     /locus_tag="ACKB38_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGL7979550.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTFGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            complement(12248..13066)
                     /locus_tag="ACKB38_000746"
     CDS             complement(12248..13066)
                     /locus_tag="ACKB38_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGL7979551.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(13053..13832)
                     /locus_tag="ACKB38_000747"
     CDS             complement(13053..13832)
                     /locus_tag="ACKB38_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGL7979552.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(13847..14338)
                     /locus_tag="ACKB38_000748"
     CDS             complement(13847..14338)
                     /locus_tag="ACKB38_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979553.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(14349..15539)
                     /locus_tag="ACKB38_000749"
     CDS             complement(14349..15539)
                     /locus_tag="ACKB38_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGL7979554.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(15551..15985)
                     /locus_tag="ACKB38_000750"
     CDS             complement(15551..15985)
                     /locus_tag="ACKB38_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7979555.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            16259..17074
                     /locus_tag="ACKB38_000751"
     CDS             16259..17074
                     /locus_tag="ACKB38_000751"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGL7979556.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNTEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            17093..17734
                     /locus_tag="ACKB38_000752"
     CDS             17093..17734
                     /locus_tag="ACKB38_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGL7979557.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            17765..18766
                     /locus_tag="ACKB38_000753"
     CDS             17765..18766
                     /locus_tag="ACKB38_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGL7979558.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSSTSIENLLANAQDIIR"
     gene            18776..19405
                     /locus_tag="ACKB38_000754"
     CDS             18776..19405
                     /locus_tag="ACKB38_000754"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HGL7979559.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(19549..19611)
                     /locus_tag="ACKB38_000755"
                     /pseudo
     CDS             complement(19549..19611)
                     /locus_tag="ACKB38_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(19793..20169)
                     /gene="tnpA"
                     /locus_tag="ACKB38_000756"
                     /pseudo
     CDS             complement(19793..20169)
                     /gene="tnpA"
                     /locus_tag="ACKB38_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(20553..23753)
                     /locus_tag="ACKB38_000757"
     CDS             complement(20553..23753)
                     /locus_tag="ACKB38_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGL7979560.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQCLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESGNPYKVYVNDKEASLTEQEKDYPETQSVFLESFDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNRDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            23976..24452
                     /locus_tag="ACKB38_000758"
     CDS             23976..24452
                     /locus_tag="ACKB38_000758"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGL7979561.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(24674..26887)
                     /locus_tag="ACKB38_000759"
     CDS             complement(24674..26887)
                     /locus_tag="ACKB38_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGL7979562.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(27288..27464)
                     /locus_tag="ACKB38_000760"
     CDS             complement(27288..27464)
                     /locus_tag="ACKB38_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979563.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            complement(27843..29189)
                     /gene="celB"
                     /locus_tag="ACKB38_000761"
     CDS             complement(27843..29189)
                     /gene="celB"
                     /locus_tag="ACKB38_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGL7979564.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NVVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            complement(29269..29775)
                     /locus_tag="ACKB38_000762"
     CDS             complement(29269..29775)
                     /locus_tag="ACKB38_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979565.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            complement(29806..30120)
                     /locus_tag="ACKB38_000763"
     CDS             complement(29806..30120)
                     /locus_tag="ACKB38_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HGL7979566.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(30130..32109)
                     /locus_tag="ACKB38_000764"
                     /pseudo
     CDS             complement(30130..32109)
                     /locus_tag="ACKB38_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
     gene            complement(32227..32541)
                     /locus_tag="ACKB38_000765"
     CDS             complement(32227..32541)
                     /locus_tag="ACKB38_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HGL7979567.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQKKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            complement(32671..32910)
                     /locus_tag="ACKB38_000766"
     CDS             complement(32671..32910)
                     /locus_tag="ACKB38_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGL7979568.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            complement(32962..34398)
                     /locus_tag="ACKB38_000767"
     CDS             complement(32962..34398)
                     /locus_tag="ACKB38_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGL7979569.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(34588..34926)
                     /locus_tag="ACKB38_000768"
     CDS             complement(34588..34926)
                     /locus_tag="ACKB38_000768"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HGL7979570.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            complement(35082..>35258)
                     /locus_tag="ACKB38_000769"
     CDS             complement(35082..>35258)
                     /locus_tag="ACKB38_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7979571.1"
                     /translation="KLELLCEEFGYKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSC
                     NTFYEALLSCSCFN"
BASE COUNT        10684 a         7617 c         5838 g        11119 t
ORIGIN      
        1 agtatattga aactagaata atacacttct actatacgac atgcctgtcg tacctaaaaa
       61 ggaaccagtt tttttcagct ggctccttct tttttatcct caaaattctg tcttagactt
      121 tctcacgaat aaccaaaacg ccatcttcca tatctgcttc cagatgttta gcatctaaat
      181 gatccaagtg gaagtctgtc accttatcac gaatttcttg ttcaaccacg cgacggagag
      241 gacgaacccc catgacttcg tcgtaacctt cttctgtgat ataatcttta gccgcttgac
      301 tgactaccaa gtcaatgtct ttcttagcca aggtttggtt aacttcagcc aacatcaaat
      361 ctacaatctt agaaaggtct tccttagtca agtgtgagaa ctcgatgact gcattaaagc
      421 ggttgaggaa ttctggacgg aagaagggtt tcaaacggtc catcaattct ggtttatccg
      481 catcttctgt caagttggct tcatagccaa atccagcatt tgaggtcgca ataatgacag
      541 tgttcttgaa gtttactgta tttccttgac catctgtcaa acgaccatca tctagaactt
      601 ggaggagaag ggtaataact tgagggtcag ccttttcaat ttcatccaag agaatgatag
      661 agtatggatt gcgacgaaca cgttctgtta aggtattgct attgtcatca taacccacat
      721 agcctgctgt tgtaccaatt agcttagaaa cagctgtgcg gtcactgtat tcagacatat
      781 ctaaacggat aatcgcatcc tgggttccaa acatatcgag tgccaattgc ttagcaagct
      841 ccgtcttacc aaccccagta gaccctacaa agaggaagtt gccgattggg cgatttcctt
      901 catcaaaacc agcacggtta cgacggatag cacgagctac aacttctacg gccttatctt
      961 gaccaatcac cttgtcttgc aagcgatgag ccatatcttt caaacgttcg atatctgaag
     1021 cttccatttg cgatactggg atacctgtca ttcgttccac agattcagcc acatcgttga
     1081 cacttgcagt cactttcata tcttctgtgt ggttttcgat tttcctttcc aattctgcaa
     1141 tgcgtgtttt atagtttaga gctgcttcaa aatcttctgc ttcaactgct ttttcttgct
     1201 tgtctttttc cgtttcgatt tctcgttcaa cagcatgcac atctgttact ggatgttgag
     1261 ccgccaagtg agcagccgtt acatcgacaa ggtcaatagc cttatctggc aagctacgtt
     1321 gaggaatgta ttgaacagaa taatccaccg ctgctttcaa gacttcgtct ggcaagatga
     1381 cattgtggtg ttgttgatag aggtcacgaa ttccttgaag aattttaaaa gtattctctg
     1441 ccgaaggagc attgaccttc acttcgttga aacgacgagc aagagcagca ttcttcaaga
     1501 tggtgttacg gtattcgtct tgagttgttg ccccaatcac tgtcaattct ccacgagaga
     1561 gagctggctt gagaatatcc gcaagtcctt tagaaccact gtctccacca gtgctaccag
     1621 caccaagaat ttggtgaatt tcatcaaaga agaggataat attccctgct tctttcactt
     1681 cattgactaa gttttggaca ttttcttcaa agctaccacg gtattgagta ccagcctcaa
     1741 gacctgagat atcaatagaa ataatttcct tgttcttgat agcagcagga acatctccgt
     1801 tcacaatggc ttgcgctaga ccttcgacaa ctgctgtctt accaacacct gcatctccga
     1861 ccaaaacagg attgttcttg gtgcggcgtg agaggatttc agatgtttct tgaatttcct
     1921 tgtttcgtcc gataacagga tccaacttgc cctcacgcgc ttctgctgtt aagtttcgac
     1981 ctagttttgc aaggacaccg ccttgtttca tacctgatgc ctgttgtggc atttgcacat
     2041 cagtttctgc atttcctggt aattgaccag tcgcacgata gtgagcaaat tcctcaggtg
     2101 tgacttcgcg tccattaatt aagtaacggc gattttcaga actgtatcct cgcataccac
     2161 ccatcaattg gttaaataaa tcatccatgt tattaaaatt attaaagttg ttattcatat
     2221 tctttacctc tttttgttta cttagttatg attactgata ttgactatct ttgaccattg
     2281 ttttaaaaaa tttagactag ctagatcgct accctacgta ctatttctgg tttttctttt
     2341 tcaagcagga gtagactatc tttacttctg aagatttcta gatgaaacat agacccacct
     2401 ctttctattt tactttaaat aagtgttcta gtaaggcttt catttacctt acgttcataa
     2461 tatactacat tggtcagaaa aggtcaagag ttttgacttt aactgaccaa tattttttta
     2521 cactcttcga aaacctcttc aaaccacatc agcttctcct tatctacaac ctcaaaaccg
     2581 tgttttgagc tgacttcgtc agttctatca accatctcaa aactgtatat taaaacgaaa
     2641 taagaacagg acaaatcaat caggacagtc aaatcgattt ctaacaatat tttagaagta
     2701 gatgtgtact attctagttt caatctacta tattttgagc agcctaaagt tagctttcta
     2761 gtttgttctt tgattttcat tgtgtattta aacacaaaaa caccctgaaa catcagggtg
     2821 ccattcttac atcaaatata aaattgctag ggtcaggaga cttgctagaa gtcccactcc
     2881 ccaaaagaag aagtcaacct tccactcgct ccaaggattt cctttaccag acaatccccc
     2941 aagctcaaaa tggtgatgta caggcgtcat acggaaaata cgtttaccac ctgtcagttt
     3001 gaaataactg acttgcatca taacagaagt tgtttcaaaa acatacacaa ttccgataat
     3061 caagagagtc cattcttggt ggagagccat agagatagct gccagcatcc cacctagggc
     3121 caaacttccc acatcaccca taaagacctt ggcaggctta tggttaaaga tgaagaaacc
     3181 gagcaaacca ccaatcatgg caagaatcac tagaagaata tccatctgac cttgcacata
     3241 ggcaataact ccataggcag acaaactaat cacaacggaa atactagcta aaccgtcaac
     3301 accgtctgtc aagtttactg cgtttgaaaa accgactagc cagaaaagag cgaagaaaat
     3361 atagaaaaat cccaaatgaa ctggataacc aaagacagac aggatatcgc caccgcgctc
     3421 atagaaaaga tagaagataa ctccacctag aagctgaaga gctaattttt gcttaggatt
     3481 aagcccctca ttgattttac gaaagacctt gagaaagtca tctaaaaatc cgaccaagcc
     3541 atacaagacc aagatgaaca aaatcattcc cacattattg ctgaattggc tactaaatag
     3601 ggcgaaaaag aaagcaacca aaacagaagt aatcaagaaa accaaacctc ccattgtagg
     3661 agtcccagct tttgcctgat gctgtttgac atcctcatgc atctgctggc ctgtaatttg
     3721 cgcctttcta taaaattgga taaaggccgg aatttctact aaagttagta aaaatgtcac
     3781 aattccagca ctgatggaaa taaacatatt agtctcctaa agttaatgta atttttttaa
     3841 tgtccttgat agctgtgtta gcacgaacat cttgcttctg cacagtagag cccgaacctt
     3901 gaaattcaag ttctatattg agccacttag caagggtctc agcagtctcc tttgtccaac
     3961 catacatatc tggaacctcc tctgctttat cagataagat aaggacttgc tggttcgggg
     4021 caagattctt cccttcttca gcagaactgt ttttaatctt cgttcctgtt cccacaacga
     4081 tgggttgtac aagattgcga cgcaattctt ctgctaaatc accaggtgaa atatccttga
     4141 cactaggcat aggataagga ctttgttgac ttacttgctc taaagcctta gctgttgttt
     4201 gaagattgag agagtctttc atagctgaag cccgctccaa gataggattg gcaaattctc
     4261 ccaactgaat acctgaataa tgttcaggtt gttggaccgt cacatacaag ataaaatcag
     4321 gattttcagc cggactcatc gatacagccg agaaaatata gtcggttaac ccgactagat
     4381 aaccaccatt tttctcgtca gcaatctgag ccgtaccaga cttgagggct acattttgcc
     4441 caggaacagt tacagttggc ttgcctgtgc tgtggttata catggttcca taaaccggat
     4501 ccgtccctac caaaaccatg ttagtccgag ttagactagc tgcatcttta gaaacaggat
     4561 ttcccacaat ttctttttga gatttccgag cagtttgatc atttggatca taaatggcac
     4621 taataaattt aggctccagc atgacaccgt cattagcaat agctgtaaag gcacgaatca
     4681 tttgcgtctg ggtcactgaa atcccttgtc caaatgagct ttgcgcaatg ttgacaatat
     4741 tatccgcagg aagctgacca gcatactcat ccgtcaaacc gaaacgggtc ggaactccaa
     4801 atttaaaacg attaagataa tcaagccagg tagcatctcc catcttttgc tcaaggaggg
     4861 tcatcccaac gttacttgag tgtgcaaaac cttgagaaaa agtcatcgtt ctgccaccag
     4921 tcaatccttc attaacgtcc caatctcgaa tcgtggcatc tgcaattttt aactcactac
     4981 tattaaagac ttctcctcct ggaaaggtat tattatcaat agcagcagcc aacatcatca
     5041 ctttcatagt ggaacctggc tcatagttac tttggtaaag gatatcacgc caaacaaagt
     5101 cctctgtaat gccttctttt gtatctgcat caaaggtcgg tcgttgcgtt gttgccagaa
     5161 tttcccctgt tttagcactg accaaagtcg ctgtcatgta ctttcctttt accttctctt
     5221 gaaaagcatc catctgggtt tccataaagg actggagggg gctggaaatg gttgtataaa
     5281 catccttacc gtccatcgtt cgttgggaaa cttgttctgt tccgggtaca atattaccca
     5341 gacgatcctt ttcataggta ataatgccgt ctgtccctgc aagaatactg ttcaaggaac
     5401 tctccattcc agaggttccc agcaagctct tgcttccatc ttcattttca tggagctgag
     5461 ctagaccgat aaaactagaa gcaaattgtc cgtttgggta actacgattg ggactggttg
     5521 taaaatcaat ccccttgacc tctgcagctt ccaattcttt tttgatagac atcatattgg
     5581 cataggtaat cccatttccc tttgctccaa aggaaacttg cttgagatta ggttgcgaga
     5641 gttgctctct tacataggat tcttccatgt ccagatactt atgaaagacc tctgcaacct
     5701 tgttaaattg tgttttttct acgtaaagaa tcttacccgt tgctgactta tagttctcat
     5761 caatgaccgc atagacatta taggaggttg catcctcagc aatcgggact ccatttcggt
     5821 cataaatagt cccacgtttg gcaggaactg tacgggtggt ttgatgaacc ttcttagctt
     5881 ccttcgctaa atctgttcca aagcgagtgc ctgtcccaat aatgaccgca aaattgacta
     5941 aaaaaatggc aaaaacaaag acagataata aactcagact ttttccaact ctgcgtctgt
     6001 tttcagccgg cgatttccga tttttggtcg cataacggat tactcttttt gtccacttca
     6061 tatcttactc cgctattcta atattttcat tgtttaattg caaatcgtgt gaattggcaa
     6121 tttctttcaa acgttctgca cgtaatagtt cattgacctc ttgcttggca tcgtccaatt
     6181 cggtcttctt ttcctctatc tgcgcattga tttttgtcaa atcattctgc acttgcaaga
     6241 gcttggtctg cataaaaata atactaatgg ctacaataag agtggttaca gcaatggaaa
     6301 agtaaaaagc tttttccaca cgcgaaaacc gtttaagttg catctgtagt atttgacctg
     6361 ttttttccat tttttctgcc atctttttcc ctcttacttg tgaatttttc tgaccacgcg
     6421 caacttggct gagtgcgagc ggttattggc ttctaactct tccgcacttg gcaagattgg
     6481 cttacgggac accaattcca tcttgggctt gagatcatct gggatgaaag gcaagccttt
     6541 tggaacttca actgttgaag cttccttgaa caattgcttg gtcaagcggt cttctaagga
     6601 atgaaaggta atcactgaaa ttctaccatc cagagccaac atatccatag cctgctggat
     6661 ggactcatct gccgctccca gttcatcatt gacttcaatt cgaatagcct ggaaaatctg
     6721 cttagcagga tgccccttct tcttgagttc cttggcaggt ttgaccaact tgataatctc
     6781 tgctaactca gtcgttgtct caatcggctt cacttcacgc gcttgctcaa tcttacgcgc
     6841 aatctgttta gagaatttgt cctctccata cttgaagaaa atacgaacca agtcatgata
     6901 gtcataattg ttcaccactt cataggctgt caggctagca tcctgattca tccgcatgtc
     6961 cagtggcgca tcctttttat aagaaaaacc acgctcacgc tggtctaatt gaggactaga
     7021 cactcccaag tcataacaaa ttccatcaat ttcctgaaca ccagcttcgc gcaaacatgc
     7081 ctgtaaatga cggaagttgt ccttgataaa ggtcaccatt cccttctcaa tgtaaggtgc
     7141 caagcgtttt tgcgcattgt caatggcatt ctgatcctgg tcaaaggcat agagatggcc
     7201 tttttcactt aatttactta ataaatactc gctatgtcct gctccgccca aagtcgcatc
     7261 aacgtagata ccatcaggct ttacgtcaag catatcaatc gtttcgtgga gtaagaccgt
     7321 tacatgatga aattcttttg tcatatctta tctattttac cacaaatccg accagcttgc
     7381 acttgtcaga caaataggac aaggagacta ctatttcttc aaaaaatatg tcaaaaatac
     7441 ttgacacttt atgtttaaaa gactataata tagtcaaata tatacgacat aaatcagaaa
     7501 ggagaacaga tgaatcgtgt gaaagaattt cgcaaggaat tgggtatttc ccagctcgaa
     7561 ctcgccaagg atatcggtgt ctcgagacag actatcaata tgattgaaaa cgacaagtac
     7621 aatccaacct tggaactctg tctcaatctc gcccgcagcc tccaaactga cctcaacagt
     7681 ctcttttggg aggaagattt ttaaaaaagg agcaaactca tgaaaaaaga aaccttcact
     7741 gaaaaactga tcaaacgcac atacggtatt tctggtcccc ttgacgaata caaacggcgt
     7801 gaggccgata gtattgggaa ccaagtcttt atcatcctct tttatctgat gattttcgga
     7861 aatcttattc cactccttct ggcctataaa taccctcaag aagtggctct aatctatcct
     7921 cctctgattt tagtgattgc cctcatcgct tctggctatg tcacctacca aatgaaaaaa
     7981 acaggcatca cagtcattga accagatatg ctgaatgaga aagaaagcaa gcaactacac
     8041 tacccaggtc tgaaagcagg tttgttcttt ggtctatgga tgttttttat aactcctctt
     8101 ctcaggatac tcatagatga aggtcaggac tattttcatt ctcttctcac tataagaaat
     8161 ggtgtatcaa gtattctcgg ttctattttc ttcggagcga gcatacagtt cctcatctcc
     8221 cgtcgcattg caaaaactaa gaaaaatcaa gatgaggatt aggaggaaac tcatgaaaaa
     8281 agaaaagaaa cagttacgtt atcctggtct gaaagcaggt tcgatttatg gaacagtgat
     8341 attttttata attccactca ttgataccct aacaagtgaa aatccaaatt ttatcagttc
     8401 tcttctaaat acaaaacata tttttaaaac tatactggga gctttctttt tcggagtgat
     8461 gatacatatt gtcgactcac ttcgtattgc aagagctaaa aaagaccagg attaggaggt
     8521 gccttatgaa atcactagct agactactga tcattcatgt ttttatcagt attttccttt
     8581 tcttcgccct tacttcagga gctatttctc atacagtttt actactccta ctcctctttc
     8641 ttcctgcgct caataaagga cttgagaaaa tacaatcaaa acggatacct gtcctcaacg
     8701 cagccctctt ctttctcctc atatcctttc cacaactttt aaccaaccct gtccaatgga
     8761 aattttcaat attcctagtc gtaaccatca tttcaagttt ggcctacttc tataactttt
     8821 atcaagtagt taaagaagta gatcaaaaac agttgattta ggagcttgct cccataattt
     8881 ctcatttcat aggcaaataa tcccacctag cttgttctag gtgggattat tttatttctt
     8941 aacagagatt gcaagtcatt ttccttcatt ttagaaagtc gactctttcc aattcacact
     9001 acaatccaag acttgttgcc tttcttcttg attgcgacct tcaatctggt caatcaaaat
     9061 ctttgtagcc tgttgtcctt cctcaaagga gggctgaacc agcgtcgaaa cacttggaga
     9121 agaaaagcaa gtccactccg tattgtcaaa accaatcaac ccaacttgtg gcaagttata
     9181 attcaactct ttgataacgg taaagactag aggtagggcc caacagttag ggataaatac
     9241 cagagttttt tcatcgggat cgatttcttt ttgtaaaaat tccttaattt gttccaaatt
     9301 cgtatgctta tcttcaatgg ttagactggc gtgacgcata ttagcatctg ttaaagcatc
     9361 cacaaaacca cttgcccgct caatccgagt actcaaacga ctcgtatccg ctgtaatcaa
     9421 gagaaaatgt tcataacctt tttcgataca ggactgggtc atgtcataaa cggcatcata
     9481 gttattggtt ttaacccagc tagtccggtg ttcatagagc tgactatcaa aaaagaccat
     9541 tttcttcttt ttctcatcga tgatacgaga atattttcgg aaattagagg tcggctgaat
     9601 aataaagccg tctactccca agagaagcat gctttcaata taccggtcct cactctcttg
     9661 gctgtaatta ctatttccta tcattacctg gtagccattc tggctggcga tatcctcaat
     9721 tcccttaaca atttggtttg agaaactgtt ggtaatatca ccaatcaaaa caccgattaa
     9781 ttttgttcgt ttggagttta agctacgcgc aacaatgctc ggtttgtaat ttgtttcatg
     9841 aataactttt tcaatctttt cacgtgtctc ttgggacatt ttttcatatt tcccgtttag
     9901 gtaaaatgac acggttgttt tcgaggtctg agccatttcc gcaatgtctt ttatggtcag
     9961 tttcttctcc aatctaaaac acctcctact aatacttatt ataacatatt cactattcaa
    10021 aatgcctatt tctagcactg caagcgaatc attctctcat ttattttatc atatactaaa
    10081 cattttccac gcatttttgt tccgtcaacg aggcacaatt tttcacctac accaatatcc
    10141 tcgcttaata acagaatcag cttgtctgtt tcatcatttt tcacttcaaa agccagagca
    10201 ttttccactt cacgatcatc tgtctggtag acagaatgac ggatgatttc aaaactctca
    10261 tgcgcaatga tggtataatc cagcatctga ttctcaaaga attggcgctt ggtgagtttg
    10321 ctacttcttt caagctcatt gtatttagga gaaatgatgg tatcttccaa atcaaaatca
    10381 acttcactcc atagtctcaa ctgattgatt ttcccatctt gataggtcac atccttgtca
    10441 aggataaact gagtcaacac ctcatgttga ccttgacacc tgatgtcatc taccaagagc
    10501 cagacatcct ctaccaacat gaggattttt ctcctgtgaa gataaggcaa atcaggttct
    10561 gctgaccaat aagccccctc aatataatgc actccctccc tttctttatg gtgacaaaac
    10621 agggagtgag gatagtattc atattcccag gatcccgtga ttctttccgg agctttccca
    10681 tctacaatgc aggtcgaatg actccaagca ctctttaaga gataacgttc atatatctcc
    10741 cgataagaat aacgcccagc atctatgaaa ataggttggc cttgatactg taagcaaaaa
    10801 ctattctcgt cactatgact atgggcactt cctagcggac catttttgaa aaatagataa
    10861 cgatgttcat ccttaatgca gacatgtcca gagtcttcaa agatcatgga cttaggctgc
    10921 caagctctct tttcaaattc ctgcagtcgc ttgacctttt ctcgccccag gaacaagagg
    10981 ctaagcaaat caactttaac atccagaccg ttaagaaggt cttcctggtt caaaaccaca
    11041 gcagacaggc tcaaaatttc tgtcgtttct gtagaatcgc tatcaccaaa agccaaagtc
    11101 cgtccatcta agcctgtcat catttgaata taggtcgcca tcttttccag caactcttgg
    11161 taactatctt gcaagtctgg aagcaagaga cacaaatcca gcaaggcttt ataaacctct
    11221 acatgataga gaatcgactg ttcaaactgg cttccatctc ctaaaatctg tgtctcaatt
    11281 tgctgtttca actcctctga agcaaaatgg taagcttctt ctagatccat cttatctgaa
    11341 aagaaatgat agatagcaag catcggaatt gtttgtaaaa tcccccagtt actaagggtg
    11401 tacttggcgc gatagtagct tttcataaag tcaatctgct tttctagact gaccaaaatt
    11461 ttctctagtt ctttctcctc tagcaagtca aatttcaaga ggagcaagag tagtttcaac
    11521 caagtaaagg aacgaatacc cgtatccaag gttctagtca tcaaggattg aggagaaaat
    11581 tctctcacct gctcaatcca atcaaataga aagaacttgc acttttgaat atagtcctta
    11641 tctccttcta ccagataccc tatcataaac tgcaagagat attcttgtcg attgagcata
    11701 taagaccatt ctggatcatc ttcaaatact tgatcccata ccatcggctg gatttgatgg
    11761 atttttgaac aaggctccat atcccaagga ctatcaaaca taaaacgatt gtccatcaag
    11821 cgttcaaggg aactcttgac tttctcatag tcttttgaac agtgcgacaa gatataatca
    11881 cgacattgat ttccatcgac tctttcaaaa aattgtcttc tttcttcttt cattatctat
    11941 taccagaaaa agaactactt aaaaagcagt tcttttgtct ttcccattac actttccttt
    12001 tctacatgga tgaccacacc ttttgcaatc tgcaaggaga ccaagtcatc ttggatagaa
    12061 atgatttttc catgaattcc agacaataac aacacttcat caccaaatgt taaagaagct
    12121 aaatactctt gtcgttgctc catctgtttg cgaagcaact tttgctgacg aatagaatga
    12181 aagcttgaca gtaaaagggg actcactgcc aagacaatca ctattccata aaacaatgtt
    12241 gtatccatta agctataatc ttaagccagc ttccgataat tccgatgata actgttaaaa
    12301 taacgagttt atatgttgtc catttctttt ctttgatcaa gtagtaaact aaaagtgtaa
    12361 atagggctgg tagaagagct ggagcaacct tatcaagcat tccctgaata cttacgatac
    12421 tttgtttagc gtctgcttta acttcccctg cagcaaaggt aatcggcacc ataatcttaa
    12481 cagatgtcgc tgccaaacca gcaattacgg ttacaccgat aatattggca atacgagaaa
    12541 tcgttgccat ctgttcgctt agtttatcaa tcacagttgt tcctagtttg tatccataca
    12601 gaccagttga caatttaatc gctgttaaaa tcgtattcat cgcaaggaag aacaagattg
    12661 gaccgacaac caagccttct tgagcaaacg aagctgcgat ggttgagaac aatggagcta
    12721 aacagaattg agaaagagaa tccccaatac ctgccaatgg tcccatcaag gccatcttga
    12781 tgctacgtgt ttcttttgcc ggacggccat tttccaacat tacaagatgc aagctggtaa
    12841 taaaaggcag gaagtgtggg ttggtattat agaattcaca gttttcttcc aaggcttggt
    12901 agaaaccttc ctgatcctct ccatagtgtt ttttcaaagc aggatacatc acattggcat
    12961 atcccaaccc ttgatagtta ctatagttaa atccattttg acaaaagaat gcccgcaaag
    13021 acgttttaag ataatcacgt tttgttaatt tgttagatcc agtcatcgtg tgcttcctcc
    13081 tctaccacat gatccgctgt ttttggcttg ttataaaatt caatcaaagc aaagatagta
    13141 cctacaattg caataccaat tgttgggatg tttagataag ctgcacaaac atatcccaac
    13201 aagacaaagg gaatcaactc tttcttagcc atcactgaca agatcatcgc aaaaccgata
    13261 gctgggagca ttttaccagc aactgtcaaa cctgtaagta ataccggtgg aatgtagtct
    13321 acgagtttca acaaggtatc cattgaaagg gcacccaagc aacccaaggt aaatccaata
    13381 aaggcaaaca accaaattgt tgcatttaga gtgaacttaa atttcttcaa attatggttt
    13441 ttcaagtgct tcatagctgt ttcaggcgca ccagcacgta cagtgtaggc gaaagtttgt
    13501 aagaattgaa tcgccacagc aatcggagta gagagggcaa gagccgcttc tggactgact
    13561 ttaccagcac tagtgatagc catcaaggta ccaaagatac caggtccgat tgggtttggt
    13621 ggaacagtac ctccagcacc aacaccgaat cccatataag ccaattcacc aatagctccc
    13681 attgcaagag cagtaggcag atcacctaga ataattccga caccaaatga cagaacaata
    13741 catctattgg tgtaaattcc taacagcatt ccactaaaac agaaagctgt ccataatcca
    13801 attaaaatcg cttgaaatac attaatcgac ataacgatct cctcttttaa atatagtcca
    13861 taatgttgac ttgaacagct ccatcatttc ctgttggagt tgttttcgta ttaaatgtta
    13921 cttgatgagt ttggctcaat tccttgaggg ccgccttgtc ctcttcaccc aggaagatgg
    13981 agcgtgtcac ttgctcttta ccaggggcat tgtgaatgtt cccaatattg atttctttga
    14041 tagtgacacc accttctacc aaggttaaag cgtccttcac atcctttaca acgataaaga
    14101 tcgtttgagc aggattagcc ttgtgaatga tatcaatcac cttttgcaaa gggaagaaac
    14161 gcatggcaac tgagtctggc acaactgttt tcatcagagt ttgttgcatc ttgtccgtgc
    14221 ttacttcgtc attggcaaca atgaccgtat tacaacctag gtattttacc caaagttgtc
    14281 cttgtccatg aatcaaccgt tcatcgatac gggtcataat aatatttggc attgtcatat
    14341 ttctcctcct accaatatag gttccagtct ttgtagaaac ggataagggc ttctaggtaa
    14401 tagtagtcac cccagatatt gccttcatcc actcctttac ctgaatgcca tgagtacaca
    14461 ccgtggagga gacttgtccc accaggggta aattgatcat ttgcataatg ttcgatcaag
    14521 gaacgaagca tggcatgcat agcatgttta taaatatctt tgtcagcatc cacctctggg
    14581 agatgtttta gcatttcatg aatcccacag acggcgatag ctgttgctga agaatctcgt
    14641 gattgatcac taccatcatt aaaaatcaaa tcccaatagg acacatgatc ttttggcaga
    14701 cgattcaaga aataattggt cacaccctta aacaagtcaa agcaggactc gtcttttaag
    14761 tgacgataag tcaaaggaat accatagact ccccatgatt gaccacgtgc ccagcatgaa
    14821 tcatcactat acccttgtct cgttacacct ttaaagggtt gacctgtctc aggatcaaaa
    14881 tagaaggtgt ggaaggacga agcgtcatca cggattacat tattagctga agcatagaaa
    14941 tggctttctg caatatcgta gtatttttga tcgcctgttt cttgataagc aaagaataag
    15001 agttggatat tgagcaagca gtcgataatc aaacggtaat gctctttctt gcccaagtct
    15061 ccccaagctt gaataaaacc acctttttct tgatagcgtt caatcaactt atctgcagct
    15121 ttcaaggttg cttctctagc ctctccatct ccatttatct tatattcagc catacaagac
    15181 ggtgtgtaca agaagccgag atcatggtga tccaattcta ctctcttatt gacacgatcc
    15241 aggaaagaaa gaacattttt atgagcgatg tttttaaatg catcctgttg actgtattca
    15301 taagccaacc acagttctcc tgtccagaaa ccattggtcc attccgtgtt atccatgatt
    15361 ggatagacat tatcaaaggt agctggcgtc gggaaatctt ccttgaaata gtcaaggttg
    15421 agttctaact gccgaataac cttatcgatt gcctggccga cttcttcttt cgtcagaagt
    15481 ggtacttcta agaagcgctc aggcgatttt attttttcaa tcgtaacctt ttttatcatt
    15541 cttttaccct ttaaataccc gattcgaaat cttcttcctc ttcctctgca tccgttgaaa
    15601 acaattcttt accatttacg actccgccct gggccgccac tactgattta ttaacaacct
    15661 catcaaagct atgagccatt ctagcaaaga ctgcttccat taacatggct aagttcaaac
    15721 ccgagagaac attcattgtc ttggctggat tttctcccat tatggtagaa gaaaccttga
    15781 atggcgatcc tcccaagaga tcacttagga ttaaaacttc ttcttcattt gaaattgcaa
    15841 gtaagatttt ttgcttgagt tcatctgctg acattccttc cacaaagtca atcgcctcca
    15901 cattttcttg attacctgca atcaattgta aagaactata aatccctgta gcaaaatgtc
    15961 catgccctac aagtacaatt ttcatctttt ctcctttctt tttaggttta ccggtttact
    16021 ttatattccc cattgtaatc ctttattttt atcttgtcaa tagttttttt aaaaaaaaaa
    16081 tattagcgct ttcattatct ttttaaccag tttaatagac agaaatttca aaatcttccg
    16141 tttttttagg aaaacaaacc tttttataaa taaaaatatt tatttattga caaaaccggt
    16201 ttactagaat ataatttggt tatagagaag tcttaacaaa tttgaaagga agtatcaaat
    16261 gacaaataca tcattctcaa ttgagcagtt ttctttaaaa ggaaaaattg ctctcatcac
    16321 cggcgcttct tatggaattg gatttgctat tgccaaatcc tacgctgaag ccggcgctac
    16381 tattgtcttt aacgatatca atcaagatct ggtcaataaa gggattgaag cttatcgtga
    16441 agttggcatc caagcccatg gatatgtctg tgacgtgaca gacgaggacg gtatccaagc
    16501 catggtcaag caaatcgaac aagaggttgg tgtcattgac atcctcgtta ataacgctgg
    16561 tattatccgc cgagttccaa tgtgcgaaat gagcgccgct gatttccgta aggtcatcga
    16621 tattgactta aacgcaccat ttatcgtttc aaaggcagtt attccttcta tgataaagaa
    16681 agggcatgga aagattatca atatttgttc gatgatgagc gaactgggac gtgaaacagt
    16741 tagcgcttat gctgctgcta aagggggctt gaaaatgttg acccgcaaca ttgcgtctga
    16801 atacggtgga gccaatatcc aatgtaacgg aattggaccg ggttatattg ccactcctca
    16861 aacagcacct cttcgtgaat tgcaagaaga tggttctcgc cacccatttg accagttcat
    16921 cattgcaaaa acacctgctg cacgttgggg aaatactgaa gatttgatgg gccctgctgt
    16981 ctttctcgct agtgatgcca gcaattttgt caatggccac atcctatatg tagatggcgg
    17041 tatcttagcc tacatcggaa aacaacctga gtaaaaatag aaagaagatc ttatgaaaat
    17101 cgcattaatc aatgaaaata gtcaagctag caagaatcac attatttacg atagtctaaa
    17161 agaagcgaca gataaaaaag gctaccaatt atttaactat ggtatgcgtg gagaagaagg
    17221 agaaagtcaa ttaacttatg tgcagaacgg actaatggct gccatccttt taaatacaaa
    17281 ggcagttgac tttgttgtta ccggctgtgg tacgggtgta ggggctatgc ttgctttaaa
    17341 cagcttccct ggtgttgtct gtggtctagc agtggaccca actgacgctt acctttattc
    17401 tcaaatcaat ggtggtaacg ccttgtctat cccttatgcc aaaggatttg gctggggggc
    17461 agaactgacc ctcaaattga tgtttgaacg cttatttgct gaagaaatgg gcggtggcta
    17521 cccaagagaa cgtgtaatcc ctgaacaacg caacgctcgt atcttaaacg aggtgaaaca
    17581 aatcacccac aatgatttga tgaccatcct taaaataatc gaccaagact tcctcaaaga
    17641 caccatctct ggcaaatact tccaagaata cttctttgaa aactgccaag atgatgaagt
    17701 tgctgcttat ttgaaagaag tattagccaa gtaaagctat tctaaaccag aaaggaacta
    17761 atggatgacg aaaatattac tgtttggcga accattaatt cgaatttcac cattagatgc
    17821 caccagtatc ggcgatcatg ttgccagttc gacttatttt ggcggatcag aaattaacat
    17881 cgcttgtaat ttgcaagccc tgggtatctc aacgaaagtt tttaccgcac tccctgccaa
    17941 cgagattgga gatcgttttc tcacattctt gaaacagcac caaatcgata ccagttcaat
    18001 ctgtcggctt ggcgatcgaa tcggcctcta ctatttggag aacggctttg gttgtcgtca
    18061 aagtgaagtt ttctacgatc gtaagcatac gagtatcagc cagattcggc caaacatgct
    18121 agatatggat tctctctttc aggggattag ccattttcat tttagtggaa tcaccgtagc
    18181 tatcggtcaa gaggtccgtg cgatccttct cctactcttg gaagaagcca agcgccgagg
    18241 aattgtcgtt tcaatggatc tcaatctgag aacaaagatg atttcagtcc tagaagccaa
    18301 gtatgaattt tctaagtttg cacgttttac tgactattgc ttcggtattg atcctctcat
    18361 gattgatgac caaaatctag agatgtttcc aagagacagt gctagcctag aagaggtgga
    18421 aaatcgcatg cgacttttaa aagaagccta tggtttcaag gccattttcc ataccctccg
    18481 ctctagtgat gagcaagaca aaaatgtcta tcaagcctat gctctagaag aactatttga
    18541 agagtctgtc caactaaaaa ctgcagtcta tcaacgaatt ggtagcgggg atgcctttat
    18601 atctggtgcc ctttaccaac tactccatca ttcctcccta aaaactacca ttgactttgc
    18661 agttgcgagc gcaactctca aatgcactct tccaggagac catctctcca cttcctcaac
    18721 tagtattgaa aatttactgg caaatgcaca agatatcatt cgttaggaga attacatgac
    18781 caaatcagat acgattattg aactaaaaaa acaaaaaatt gtcgctgtta ttcgaggaaa
    18841 tacaaaggaa gaaggactac aagcctcgat tgcttgtatc aagggcggta tcaaagctat
    18901 tgaaatcgcc tataccaatc agtatgcagg acaaatcatc aaggaacttg tagacttgta
    18961 tcaggacgat cagagtgttt gtatcggtgc aggtactgtg cttgatgccg taactgctag
    19021 agatgccatt ctagctggag caaattacgt tgtttctcca tctttccatg ctgaaactgc
    19081 gaaaatgtgc aatctctaca gcacaccgta cattccaggc tgtattaccc tcacagagat
    19141 cacgactgca cttgaagccg gtagtgaaat catcaaactc ttcccaggta gtactctcag
    19201 tccagcatat atctctgcag tcaaggcacc gatcccacaa gtttccgtaa tggtaaccgg
    19261 aggagtcggc ctaaacaaca tccctcaatg gttcgctgct ggtgcagatg ccgttggaat
    19321 tggtggcgaa ctcaataaac tcgcttccca aggcaacttt gaccgcatca gcgagattgc
    19381 ccaacagtat attacactca gataaaatca taactacccg tctaacgggt ggtttatctc
    19441 agagctataa gcccaaatca tcagccagcg cctaaagacg ctggctttca cgttgttcaa
    19501 gccttattgc tcttgactcg tcacttgcct ctttaagaga ctttggtatt acttaccact
    19561 atccctaaag ggatcctcat attcttttac actcaattta tctagtgcta tagtagattg
    19621 aaactggaat agtacacctc tgcttctaaa acattgttaa aaatcgattt gactgtcctg
    19681 atcgattttg tcctgttctt atttcatttt actatatatc atactttact cgttctcaaa
    19741 ttttcatact catgaagaaa tcatccactc gataatttct ttaatcttga ctatatttct
    19801 taattgtggc ttcattaagc cctactggac ttacataata accttcctcc cagaaatgcc
    19861 gattcccaaa cttgtacttg agattggcgt gtttgtcaaa catcatgagt gcacttttgc
    19921 cttttaaata ccccataaaa cttgaaacac ttagcctcga cggaatactg actaacatgt
    19981 gtacatggtc tggcattaag tgaccctcga tcatttcaac acctttataa ctacacaagc
    20041 gatgaaatat ttcgtctaaa ctacttctat attgattata gatgactttt cgtctatact
    20101 taggggtgaa cacaatatga tagaacacct ccactttgtg tatgataaac tatgagtctt
    20161 ttgtgccata ttttttctcc tttcgcttta caattggatt gaacaccttt attgtatcgc
    20221 gtttggagtt tttttggtat aaccttcgac gcgcacccgt atagcgggtg gttgttttgt
    20281 ctcgcacctc acggagcgag acggactaat atagtggagt gaaataggat acgaacaaat
    20341 tgattaggaa aatcaaatga atttatagaa atcttttagc agttataacg ttctattcta
    20401 gtttcaaaac gctatagtca cataataatg aagtaaaaaa ggataagtat caacttatcc
    20461 ttttttaaaa gaaaaatccg aagatatttg gccttcttcg gattttttct attttccaca
    20521 gtttcatgta attcatctag atgatgaaca aattagttgt tctttcctct acggaataga
    20581 taaaatgccc caagtagcaa gaaccctaga cttgccaaga ttgactgacc ttctcctgtc
    20641 tgagggagat tcttttgatc cgaatggttc ttttcctctt cagatttttc cttttctttt
    20701 gaattctgta cttgtggctg agctgcttgc tctagctttt taaagacttc ctgatctgga
    20761 gctgattcct gggtttcagg attatagtag gcaatcttat attcatcccc ttcttttcga
    20821 atggtataga ctccacgttt caaaacttgg aattggttgg aaatagtaga gacagaatca
    20881 tcatatttca caatgcccca aactccttgt ttagcatcat aaacagactg aagggtttcg
    20941 ttattttcga tgaggctact ttctaactct tttatcattt gattgaaggt ggcacgatcc
    21001 acgttaggaa tgagcatata gccataagaa tctctatttt gcttatgagc ctgactaatc
    21061 gtaagaaatt cattttcaac ttccttgtct gactgtcctt cattgatatc cttccaggct
    21121 cccttttgca aagccttact catactgatt gaactcttct taaagaaaaa gtaaccaata
    21181 ttctttttcg aatcgaacga ttctaaaaag acactttggg tttcaggata atccttttct
    21241 tgttctgtaa gggaggcttc tttatcattg acatagactt tatatggatt acctgattcc
    21301 agttttctct ggtcaattgt agttgcagca gtatctgttg aagtgttttg gatattgctt
    21361 cctaaaaagg cgatcttatc ctttagcata aaccagctct tatgagcagt caatgtttga
    21421 ttccagttgg tgaaatccat ggttgctgtc gcattggcat catctagttt gctcgttcca
    21481 acgaaagcag acggtaaaac tttacctgta tcgctatccg ctctcttagc atccgtctct
    21541 gttgtaccag gcatcttata tggattaact gttggccagt agccatcgct atagtgactc
    21601 aaatcgccat tgtaaagata gaacatccca tcactcgtat accaaccacg tttattttcc
    21661 ttgttcatgt gttcgtaatt caaggtacga ctggaaaaga gtgacaagcc aaatccaaac
    21721 cctttctctg cattgtacat ggctgtttta tccatcttgt taaaggcaga taggtaactt
    21781 ggtcttggaa cacttgcgac tcctgcatca cttaacaagg attgcatcaa actgatatcc
    21841 ttataagtct tcaaattctt aaagacatca taataactat ccgattgaac aatggtcttc
    21901 acaagactct gcaaacattg tttggtttct ccttcagaca tatccgctat tcggtgaatc
    21961 cctcttagta cttctactgc ggccacgtgc ccctcgctat ttgcacgact gatcgagcgt
    22021 ccacgactca tatccatcaa ctctccattc accagcaaag gagcaaacga tttatcaatc
    22081 cagtggtaca tggtttgcat tttatcttta tcgattggat tcttggtctt ttgaatgact
    22141 ggcaacagtt gagacaggcc atcaatcaaa acattcccat aagcacccgt ataggcaaca
    22201 ttggtgtggt cgatatagga tccatcttga taaaaacctt caccttggtc taccaacttg
    22261 aacacttgct caatcgagcg aatggtagaa gaaatttctt gatcatcctt acgcagtaaa
    22321 ccagctatta cttttaccct tcccatatca actaagtttc cacctagagc cttgaatggg
    22381 ttatcagtcg tctttcggaa atgttcggga tctggtacaa atttttcaat cacatctgta
    22441 tattttttaa tttcctcatc agagaagtat tctttcatca gagacaaggt attgttgatg
    22501 gcacgaggtg taccgatttc ataatcccac cagttcccaa caatgctctt ttcactattg
    22561 tagacatgtt tatgcatcca ttccatggaa tccctgactg ttcgaacgac agtttcatct
    22621 tgataataac gagaagaagg attggtcact tgcttggcca tctcctccaa tttccgataa
    22681 gtggcagtca gatttgcaga cgttttataa tttgaaaatt tttcccacaa ataggtgcgg
    22741 tccgcctgac ttgaaatact ggataggcta tcagctacct ttccttccaa ttcctggttt
    22801 aatttggcca tctgttcatt tttagaatca tagtattgat tcccagcgat gatgccattc
    22861 cagtcatcca aacggtctgt gtatgcatcc ttaacagagg ccagaatctt caaaggaatc
    22921 tttttcactt ccttgccatc tttactgaca atgacattgg ttgtcccttc cttaagaggt
    22981 tctaaaattc catttttgac tgaagcaacg tcaggatttt ctaccttata agtatagtcc
    23041 gcaagagaaa aaacatgttt ttttccaatt ggtaaatcaa tcttttcctc aagctgttta
    23101 tctgtttgag aatcctcaga aagctggtct gctacctcta ccagctcaat atccttaaag
    23161 gaaacagtcc cagttcctgt ttcatagaat aactccagct tgattttatc aacatctaaa
    23221 gtcgggctat agtctgcttc aatggtctgc cagtcctttg ttcctgacgt cgttgcagaa
    23281 ttccacaatc gcttgtcctt accactttcc tcaatgatac gaactttggc aatcccgatt
    23341 ttattatctg ttttaatctt gaaacgcagt ttatactttt tcttagcttc aataggaacc
    23401 atacggtgaa gcgctgccct taatttctca tggcttgaga tagtgatagc cccatcctta
    23461 gcctcaatga ctcgagttga ggcatctgca ctattcttct ggtctaccca agctgaccac
    23521 ccctgagctt tgcttcctgt ccacggactg cttccgtcct ccgtctgacc aaaatcacca
    23581 ttttcaacta aattttttgt atccctttgc tgatactgaa cttccttctg gctttgctga
    23641 atggtattcg tagtcgtttc ttccgctcca attgtcatat aatggtttaa taaaaatcct
    23701 gataaaacaa gtgaagacaa actcacaatg agcttctttg tttttgtttg catgtataca
    23761 ctccaataaa catattgtaa aatcaatagg aactaaatct caaattagag atttatagca
    23821 actaagtaaa ccggtttaat taaatcatac catttttaaa gttatttgta aacggtttct
    23881 atcatattga aggcttattc ttcaacaata tcttccatcc gactcgacga aaaaagtaaa
    23941 aactgttaca atgtagaaaa agaaaagagg ccctcatgac ttcactttat gatttttccg
    24001 ttttgaacca aaacaaccaa gcaactccct tggatagcta tcgtggcaaa gttctcttga
    24061 ttgtcaacac tgctactgga tgtggtttaa cgccccagta ccagggactt caagaactct
    24121 atgaacgcta tcaagatcag ggcttcgaaa tattggattt cccttgcaat cagtttatgg
    24181 gacaagcacc cggcagcgca gaggaaatca acgccttctg tagcctacat tttcaaacca
    24241 ccttcccacg ttttgccaag attaaggtca acggtaagga agcagaccct ctctatgtct
    24301 ggttacaaga ccagaaatcc ggcccactag gaaaacgagt cgaatggaat ttcgctaagt
    24361 ttctcatcgg tcgagatggg caagtctttg aacgcttttc ttcaaaaaca gacccaaaac
    24421 aaattgaaga ggcgatacaa actctactat aattcacaat ctcactatga ttaggtttcc
    24481 tttaacctga tgaatagtga gattttttga tgggctttga cttaaataga aaaacacccc
    24541 atgatatgaa acatgaagtg ttgtaaagtc tatgttgtag gtgcttattt cacaatttca
    24601 atgtgaccag tgataacgaa taccatacag aatcttcata tacactaaac aaatgacttt
    24661 ctaattattt caattagttt tggctagtaa atatcatttc caacaaacgc cctctcaatt
    24721 ccttatcctg atgatgcaag atattcatta agtcatgaga gtttttcgca ttgatgaatt
    24781 gatttaacaa tctatctttt aattcatatg gaagagaagc tgtctttagt agtctaaaaa
    24841 cttcgtcatt taaagatgtc cttttattat ctttccattc aaatttagct gtatcattct
    24901 tatttggcaa ttcaattata gacacattcg ttcctttaaa atgaattcta tgttttctat
    24961 tgcttggaac gatactagaa tctccttgta atgctaactc taccattccc atttcccaat
    25021 cgattgataa tcttgtttta tatctttgac cattttgatc ttcaagcatt tcaaaagaat
    25081 gttgttttcc tgggaataca taccaatcta caacttcagg taaatcaaca cccataccta
    25141 tctcagaacc aaccaaggga atgattgcac cactttttgc aaacacaggc gtagtcgaga
    25201 tgtccctata aacacttaac ttcacaccac ctgtgtattt tttctctgaa aagaagtcat
    25261 accattcacc ttcagggaac catacatcta cttttgcaga ttggaatgtc aaatccatct
    25321 tttctacaat gggagccacc atcagttctg ttccaaaaaa gtattggttt ggaacattat
    25381 agctctcatc attctctgga tagaaataat agattggact gattaatggg gcaccttcct
    25441 catgtgtctg tacattcatg gtatatagat agggaatcat ctgatgtctc aaacgaaggt
    25501 atttcttcat aatcttagat gttgtttctg aaaaaaacca aggttcttta ctattaaaag
    25561 gacttctaga actatgtaat cgagtaatcg gactaaaaac accaaactgt agccatctag
    25621 tttgtagctc ttcgtcataa tcccccaaca tatgtccacc gatatcatga ctccaccaac
    25681 tataaccgat attagatgct gtcgctgtaa aatagggttg aaatcttaag gaattccaac
    25741 taataatagt atcccctgaa aaaccaacag ggtagcggtg actaccagga cctgcatatc
    25801 ttgataaaat caaaccacct tctgcatttt tacaactatc ctgatagtga taatggttta
    25861 aaagccaaag tggatctagc ataccttgtg tcccttgttg ccagtcaatc caccaaaaat
    25921 ctactccctg cttttctagt tcataatgaa catctttaaa gtaggcttcc ctaaaagagg
    25981 gattaaaaaa atcaaaaata gcaggttctt ctagttctac atttaacccc aaccgttttg
    26041 cgatttgagg ataagcttct tcataagccc gtatcccatc agcaggatgg acatttaagg
    26101 agagttttag ctttctatca tgaagttgtt gcaataactg ttctggattt ggtattaagt
    26161 ttctattcca actatatcct gtccagccac ttccaaagcg agctggaatg tcagttatat
    26221 gccaatccat atctaacaca ccgatagata atggaatttt ctctgtttca aatctgtcta
    26281 ttaaatccaa gtattcatcc gacgtataag gccaatatct actccaccaa ttgcctaaag
    26341 catatcttgg caacaagggt gttgaaccag tcaaatggta aaaatctctg attgctcctc
    26401 tataatcatg cccataggca aagaaataca ggtcaatttg attttctctc tcaatataac
    26461 cagattgttc atcccaaata aatccttgag aatcatccaa taaggctata ccatttcggc
    26521 taataattcc atcttctaac gagattgctc catctgcctt atccagagtc cgagctgttc
    26581 cttttaacgt ttcaatagat tcaccaaaat accagcgact accatatacg gcaaaatttc
    26641 cttttaattc tataaataaa ttttcggcgt taaattctcc tttattaaag tgcagatgaa
    26701 aatagtccgt cataatatct agtacgtttg atgtctcgat ataatctaac gaaatttggc
    26761 caaaatctct attatagata agttgtgtcg ttctatcctc aaaacttcca gtttgagagt
    26821 attctaacct tactagcttg tctgttaata cagagattcg ataaaactct cccttaaaaa
    26881 ttttcaattt gttttcctcc ttttatggta gcataaaaac agaacgcacc atttttgatg
    26941 cgtttttcat tattctgaat gcaatgttct atctgttata tctatgacaa ataatagtca
    27001 attgaaaaaa tgcagtggac aaaatatctt ttaacaaacc aagagtttat taaagagtta
    27061 tcacttttca acttttctaa gcttatgcag ttgtgaaaca aactactttt aaactattaa
    27121 ctaagatagg attgataaat aatttcaaac tcttactagc aatcatacga tattcaagct
    27181 cacgtgcttt tttccttcct gcttatttct tagaactgaa gaacccggat cggtatataa
    27241 attatccgga tcaacatagt cataagattc ataacagttg cgcttcatta agtcatcccc
    27301 agagcaagag cttcatctcg taatttttca acatcactaa ccgtaggtcg ccatccttca
    27361 atcatatttg tacttaaagc ataccaaaca ctcttaaaaa cggatcggtt ttcaaaagct
    27421 attcccatga ttgtcatctt ttctttatct atatctaagg acatatgcta cctcctttag
    27481 atacattata ccatgtttct ctgtagcttt taaaaatttt attttgtttg tcatatctaa
    27541 gttttcagca cgcttatcct attttataag cctcaaaccc aaatataaaa cgcattcttt
    27601 ttgctttttt actattgtat cgtattctac gataacatac tttactttat tgttttttta
    27661 aataacagca gttccctgtt tatcaactat tcgaactact ttctattttg cttcataccc
    27721 tacatagcga aaaaatatga aaaagcagag aagaatatct taaaaagacc tcttcactgc
    27781 taatattaac actcattatt taaactatat ggattctatc atcgagtata cttttttact
    27841 tattagatac cttgctcttc tttcaccaat ttttgatcat atacacggat gaatggaaga
    27901 tagactagga atgctgcaaa tgcacatact agagcaacta atacagctcg aagatctgct
    27961 gtccctaaga aagctccaat ccctactgga gttggccatg gaacctgtgc gataattggc
    28021 ttaataaagt ttagagaatt cgctacgtaa taaatagtag cagtaaccat tggtgctaaa
    28081 ataaatggta tagccaaggc tggattatag ataataggta atccaaaaat taatggttca
    28141 ttaatattaa ataaggctgg aactacagat gctcgtccta ttgctttaag ctgttcagat
    28201 ttagaggcaa aagcaatata taaacatagt cctaaagttg caccagaacc acctgcaatt
    28261 acaaacatat tagaaaattc acctgcaaca gcgaagtgcc cgccagcagc attttcagcc
    28321 atgttagcaa gagcaattgg actaacaaat gcaaaaacaa tgttcgcacc gtggatacct
    28381 acaatccaaa gtagttgagt caatagataa ataatcatta aaccaatcca cgaattagtc
    28441 agattggata caaaaccaaa tggaattgca atgactttaa aaatatctgt tcccattgct
    28501 acaagaagac cgttgataaa gataacaaca aatgcaacaa caaatcccgg aaccaaagcg
    28561 gtaaatccac gagaaactcc ttctggaaca gcttcaggca ttttaataac ccaattatgt
    28621 ttaacacaca tacgataaat aagaacagtc acaattgcca taatgattgc ggtaaaaatc
    28681 cctgttgtcc caaaacgtgc gactacattt cccattgccc atccatctgc aattactgca
    28741 ccttctttta gacttgtcac agtcttcatc attccaccat caaaaatgat ttgcggtact
    28801 gtcatgacaa aagccatcaa ggcaagcaag gcaccattaa gaggattcat attgagttct
    28861 tcttcctctg cataaatttt tgtcaattca tatgcaagtg atagaacgaa ataaagagat
    28921 agagaaccca tagtcgcata gtttgcaacc atgtaaagtg atgtgaattt atcaaatgaa
    28981 gcagagaaaa tatctgccac aattggccaa aatgagaaag cttgtggcaa aatactgaat
    29041 accaaaaaca ttgatcctac aatagtaaat ggtacagcag ccatacctgc agccgtgata
    29101 gcacgtacta ctttaaactg agcaagtttg cccattggtc ccataacatg gttttcaaga
    29161 aaaccaaaca acccgttttg ttgatccata aatagacctc cttaataaaa cataataatt
    29221 tttactttct aaagactagt ttcaaataca aattatacta gatcaggatt ataaactaag
    29281 tgagttcttt tccaattgga caaattgttg ataagcctta tctgttcgtt tataaatttt
    29341 tttaattctt ctaatgtcta acaaactcag aactaaacct aatagaagaa ctacaaaaac
    29401 aaataaacgt gctacttggt tattttcaaa aatcggaaaa agattcttaa accaacttgt
    29461 ccaagttaaa acaagtaatc ctattgaaat aagcatttgt attctaacaa acattagtgt
    29521 tattcccaac ttttctttcc tatttccata aagtttaaat tgttcaacag ttgctaaaat
    29581 agaaaatact atgagcataa tggggaaaat aataataggc gagggactaa taaactgact
    29641 caaaagccaa taaatattcc caaaaaagaa gagtgctatt gaataacgta gaagaagata
    29701 tcgattgaaa aaagtattag ttagagccat ctctcgacgt tgttgttcaa tcttttgtcg
    29761 ttctttttta tccatatcat ttcctcctta tataacaaca catatttagt taactttctt
    29821 ataaagagct aacatttcct ttgctacttc taataatgtc atagtggtca ttaaatgatc
    29881 ttgagcatgt accatgataa tttcaatttt aatttccact ccacttgcgt attcttgcaa
    29941 gagtttggtt tgtgcatgat gcgcttcaag aattatctca tttgattgat ttaatttact
    30001 ttctgcatca tcaaaactac cttctctcat ttttgcaaat gcttcatgta tttctgacct
    30061 tgcatttccc gaatgcagga taatttcaaa tgctgcaacc tgcagttcct cttgattcat
    30121 ataaacctcc tattttatct tctcaaatat gttaataaaa tcttcaaagt tattgcaaga
    30181 tattagctga ttttgcaatt catcattctc tgtcagagag actatctttt tagtcacagt
    30241 tgccaaacct tcgttcccat atattgatgg agatagaaga aatactagct ggacatgtga
    30301 actttgatta tcccagagta acgaatcttt acaaattgca accgaaacct ttccctctgt
    30361 accaaagggc tgaataggat gcggaactgc aattttttca gaaaaaacaa ctgaacttaa
    30421 ttcttcgcgc tgtttaattc cataaagtaa agattgttca aactcatttg attcaccaac
    30481 agataaactc tcaaccatct tttcaagtaa atttaccttg tctgattcag tacatattaa
    30541 aaagttttct ttactaaaat actgtctaaa gccgttgttt tcaaatttgt taatctttga
    30601 tgattgtaca taactagaaa cttgcatcta atccatagct tttctaatca tttccatctc
    30661 atcactctta agaaacacac taactttaaa aactgggatt tgaaaatata gatttgataa
    30721 atcaatagct gacactataa aatctattcc tttaagtttt tcttgattca attcatagta
    30781 gcctattaca tcaacaactt ctactcgctt cccaaactcc gtttccaaac gatttcttaa
    30841 catttgggct gcaccaaatc ctgttgcaca aatagcaaga atattaaact tagtactctc
    30901 tttgctacgt tccatagcag ctaaaaagtg aagacttaca tatgctactt catcatctga
    30961 tattgtccac tccaagaact tgtccatatt tgcaagaatt tctctagtca taaagaatat
    31021 atcactataa ttctgtttaa tttcatctac caaagggtta tttaaggtaa tccggctttc
    31081 taaacgtact tgtagtgtca ttagatgagt tatcaatcct tcaattagtt ggaaatctga
    31141 agaaaagtta tacatatcat ctaatcctaa attctgaaat gttttaaata aagatttttt
    31201 taaaacttct tcagaaatat tcttctgatt tttttgacat tgttgactct tagctaacaa
    31261 atgcaaagta atgtagtcta tttcctgaac tggaaattcc tgatttgtta cttctcttac
    31321 tttagaaaga attctttggg caacctttct ctctattgca tcatcagtca tctgacagtc
    31381 tatatttttt atttcaaatc cggattttaa acgaatcaca gacaatgcta tgtgaactac
    31441 taaattctgt agtacaaaat cagatagttt taggttggcc tcttggcatt catccaaaac
    31501 aattctagca aattcttcta atggaacagt ttgatcaaaa aagttaaatt ttacatagca
    31561 atgtattgtt ttaaaaaatt gattctctag gaaataattt atgataaaac gtcgtttatc
    31621 acgttcctcg cctgagacat aaactccttt attcgcccta ctctcaatgg acaaattata
    31681 ctctgataac atcactcgta tctttctgaa atcatgagat aatgttgaac gactaacgta
    31741 aagttcatca gctaaatcat caaaaagaac tggaacttgc tcaaataata atttatttaa
    31801 gataaatact aaacgatcat caccttttga aaccgcagtt ttcgtatagt cttcttccag
    31861 ttcataagtt tgtctaaact cctggtaagc gccttgattc tcaaaaaata tttgataccc
    31921 ttgaccttgt tttgaaatca accggactcc ttgaataatc attgtcttct caattaattt
    31981 cagtacatta cggacagttc tatctgaaca ggataaatat tctgccagtt ctttgcttgt
    32041 aacaaaacgt tccttatttt ttattaaaaa ttgaaggata tctttctctt taatgtttaa
    32101 cacattcatt ccctcctaaa acgtatgttt tcatatattg aagcatatta tacacttaaa
    32161 tcagtttata tcaaactcaa aacaatttat cttaacctaa atatttattg acatttcatg
    32221 tgttcatcaa atattctcaa gaatcaaatt agccattttt tcaattccca ttggaatagg
    32281 aatataggct tgaggaggta tttgtacaac tggttttcct gctttagaac cagcctcttc
    32341 aaattgctta aagtacattt ttttttgagg actgacaaga tacaaatcaa aagctgctgc
    32401 tgcgatagct ttccctcctt cagtagcact aatagcatca actacaatat ctttcccttt
    32461 tccttttaga aactctgttg ttttctgtgc cataagtgat gaagacattc ctgctgcaca
    32521 aataattaaa gcttttgcca taatattttc tccttttctt aaatccaatc aaagctgtgc
    32581 taagttggct tatttgttat ctatttttat tataaaataa agcgtttcca atgacaattc
    32641 cctcattttc ctaaatgata tggaaaaaaa ttatttatac ttcaatttat aaaataaaat
    32701 tattcctgag agtagaaatg aaacactatt tgctaaaatc aaaggcaagt ctcctatacg
    32761 aataccatga gcaagccaca atgcaatacc aataacttgc ataacataca tacctagagc
    32821 aatagatcct gtgtcctttg tcttaactac acgaaaaact tgtggtaaaa atgcaaatgt
    32881 tgttaaaatt gctgcaatac ttccaatcat atgtcacctc aatatgctaa acaaactgag
    32941 aataatctca gtttgtttat actattctac tgattcaccg ttagatgaaa taacttcctt
    33001 ataccagcca aaagattttt tcggggaacg attataactt cccttcccat tatcatcttt
    33061 atctacataa ataaagccat aacgtttccg catttcaccg gtaccagctg aaaccaaatc
    33121 aatacatccc catggagtat aacccattaa atcaacacca tcttcaacta cagccttttt
    33181 catttcacga atatgggcac ctagatattc aattctataa tcatcatgta ccataccatc
    33241 tgctgcaact tgatctatag ctccaaaacc attttcaaca ataaagagtg gtaagtgata
    33301 gtggtctgta aaccaattta acgcataacg caaaccttct ggatcaattt gccactccca
    33361 ttcagaagcc ttaacataat tatttttcac taaatcttct gtttcaagat aatcaaaata
    33421 aggattattt tcacgatgag agtcgatagc aaaggacata tagtaactga aaccaatgta
    33481 atctacagtc ccaccaagta aatcttcttt atcctgggca gtaaaatcaa ctgaaatacc
    33541 ttttcgttcc caatacttga aaatatgctc aggatattta cctaaaacat gcacatcagc
    33601 aaaataataa cgcttctgca tagctttcat tgccattaag atatccttag gattgcaagt
    33661 aactggataa attggacaca tcgcaatcat acaacctatt tgaaaatctg gattaatctc
    33721 atgaccaatt tttacagctc gtgcagaagc aactaattcg taatgtgctg cttgatacat
    33781 aattgcttct ctattatcac cttcctcata tacaatacct gagttagtaa atggtgcaaa
    33841 atcttcctga taattcgctt gattattgat ttcattgaaa gtcatccaat atttaacctt
    33901 atctttgtaa cgtttaaata cgacttctgc aaaacgagca aagaaatcaa tcaatttcct
    33961 atttttccaa ccaccatatt cggtcactaa gtgataaggc atttcaaaat gagatagagt
    34021 gatgacaggt tcaataccat tctttaagca ttcatcaaaa agattatcat aaaactgtaa
    34081 tccttcttca ttcggctcta actcatcacc ttttggaaag atacgtgtcc atgcaataga
    34141 ggtacggaag cacttgaatc ccatttcagc aaaaagtgct atatcttctt tataacggtg
    34201 ataaaaatct atcgcctcat gatttggata atatttaccc tctaaaactc ccaaagtaat
    34261 ttcacgagct actccatgac gaccagcagt cataacatca gcaacactaa ttcccttgcc
    34321 accttcttgc catccacctt caagttgatg agcagcaaca gcaccacccc ataaaaatcc
    34381 atctttaaaa gtagtcatct tttttcctcc tgactttgat actcttatta taaaccttaa
    34441 accaaaagat gaaaacgcat tctttttcct tattgttaag gaaagaagta atttttaatg
    34501 gaaatagaac aatatcttct tgtattctcg taatgatatc tttacgattt tcaatacttt
    34561 caaactacaa aaactctcac aataattcta attccctgtg tctataaacg acttatcgct
    34621 ttctggcatc ccagaatcat cttctatata acgttcaact tgcatctgca agtgatattt
    34681 ttttcttaaa tctaagattt tctgcattgt ctttgattga taatgtttat ctaaagtttc
    34741 ttgatttatc cactgatcaa taaggagaat agttccctct ttttcaattg gtaaaaaata
    34801 ttcgtatttc aagttacctt tttgatttct aatttcttta acaaggccac tatcaagcat
    34861 ttctcttgca aactttattg cactatctcc atcaccttta taatatacat gaatagtcaa
    34921 tgtcatctta tatcctccaa aatcatcctt caattttaaa aaaacaagtt tagatgagga
    34981 tctaaacttg ttttttatga actaattatc taacgtttcg ccattacttt caatcacttc
    35041 tttataccaa taaaatgatt ttttcttata gcgatttata gtcaattgaa acaagagcag
    35101 gacaaaagag cctcataaaa ggtattgcaa cttggtaata cctttttgag gtgctttttg
    35161 atatgagccc atgttttctc aataggattg tactcaggtg agtagggagg aagaggtaaa
    35221 agtttatacc caaactcttc acacaagagt tctagctt
//
LOCUS       DBDESG010000016        34104 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.016, whole genome shotgun sequence.
ACCESSION   DBDESG010000016 DBDESG010000000
VERSION     DBDESG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34104
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.016"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(115..351)
                     /locus_tag="ACKB38_000770"
     CDS             complement(115..351)
                     /locus_tag="ACKB38_000770"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGL7979572.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(364..837)
                     /locus_tag="ACKB38_000771"
     CDS             complement(364..837)
                     /locus_tag="ACKB38_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979573.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(855..1040)
                     /gene="briC"
                     /locus_tag="ACKB38_000772"
     CDS             complement(855..1040)
                     /gene="briC"
                     /locus_tag="ACKB38_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGL7979574.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1199..1966)
                     /locus_tag="ACKB38_000773"
     CDS             complement(1199..1966)
                     /locus_tag="ACKB38_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGL7979575.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1963..2829)
                     /gene="accD"
                     /locus_tag="ACKB38_000774"
     CDS             complement(1963..2829)
                     /gene="accD"
                     /locus_tag="ACKB38_000774"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGL7979576.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2866..4233)
                     /gene="accC"
                     /locus_tag="ACKB38_000775"
     CDS             complement(2866..4233)
                     /gene="accC"
                     /locus_tag="ACKB38_000775"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGL7979577.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4245..4667)
                     /gene="fabZ"
                     /locus_tag="ACKB38_000776"
     CDS             complement(4245..4667)
                     /gene="fabZ"
                     /locus_tag="ACKB38_000776"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGL7979578.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVVIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(4664..5149)
                     /gene="accB"
                     /locus_tag="ACKB38_000777"
     CDS             complement(4664..5149)
                     /gene="accB"
                     /locus_tag="ACKB38_000777"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGL7979579.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5152..6387)
                     /gene="fabF"
                     /locus_tag="ACKB38_000778"
     CDS             complement(5152..6387)
                     /gene="fabF"
                     /locus_tag="ACKB38_000778"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGL7979580.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6409..7140)
                     /gene="fabG"
                     /locus_tag="ACKB38_000779"
     CDS             complement(6409..7140)
                     /gene="fabG"
                     /locus_tag="ACKB38_000779"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGL7979581.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(7174..8094)
                     /gene="fabD"
                     /locus_tag="ACKB38_000780"
     CDS             complement(7174..8094)
                     /gene="fabD"
                     /locus_tag="ACKB38_000780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGL7979582.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8087..9061)
                     /gene="fabK"
                     /locus_tag="ACKB38_000781"
     CDS             complement(8087..9061)
                     /gene="fabK"
                     /locus_tag="ACKB38_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGL7979583.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9180..9404)
                     /locus_tag="ACKB38_000782"
     CDS             complement(9180..9404)
                     /locus_tag="ACKB38_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGL7979584.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9464..10438)
                     /locus_tag="ACKB38_000783"
     CDS             complement(9464..10438)
                     /locus_tag="ACKB38_000783"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGL7979585.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10438..10872)
                     /locus_tag="ACKB38_000784"
     CDS             complement(10438..10872)
                     /locus_tag="ACKB38_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGL7979586.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10966..11751)
                     /locus_tag="ACKB38_000785"
     CDS             complement(10966..11751)
                     /locus_tag="ACKB38_000785"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGL7979587.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12076..13440
                     /locus_tag="ACKB38_000786"
     CDS             12076..13440
                     /locus_tag="ACKB38_000786"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGL7979588.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKNQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13443..13814
                     /locus_tag="ACKB38_000787"
     CDS             13443..13814
                     /locus_tag="ACKB38_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGL7979589.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14027..15301
                     /gene="serS"
                     /locus_tag="ACKB38_000788"
     CDS             14027..15301
                     /gene="serS"
                     /locus_tag="ACKB38_000788"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGL7979590.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(15326..16225)
                     /locus_tag="ACKB38_000789"
     CDS             complement(15326..16225)
                     /locus_tag="ACKB38_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGL7979591.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLIGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16335..16883)
                     /locus_tag="ACKB38_000790"
     CDS             complement(16335..16883)
                     /locus_tag="ACKB38_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGL7979592.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17101..18423)
                     /locus_tag="ACKB38_000791"
     CDS             complement(17101..18423)
                     /locus_tag="ACKB38_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGL7979593.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTNFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18514..18602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18766..21102)
                     /locus_tag="ACKB38_000792"
     CDS             complement(18766..21102)
                     /locus_tag="ACKB38_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGL7979594.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQLLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21206..21754)
                     /locus_tag="ACKB38_000793"
     CDS             complement(21206..21754)
                     /locus_tag="ACKB38_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGL7979595.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(21751..22053)
                     /gene="zapA"
                     /locus_tag="ACKB38_000794"
     CDS             complement(21751..22053)
                     /gene="zapA"
                     /locus_tag="ACKB38_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGL7979596.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22140..23021
                     /gene="rnhC"
                     /locus_tag="ACKB38_000795"
     CDS             22140..23021
                     /gene="rnhC"
                     /locus_tag="ACKB38_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGL7979597.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            23025..23639
                     /gene="lepB"
                     /locus_tag="ACKB38_000796"
     CDS             23025..23639
                     /gene="lepB"
                     /locus_tag="ACKB38_000796"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGL7979598.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            23719..23841
                     /locus_tag="ACKB38_000797"
     CDS             23719..23841
                     /locus_tag="ACKB38_000797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979599.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            23918..26284
                     /locus_tag="ACKB38_000798"
     CDS             23918..26284
                     /locus_tag="ACKB38_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGL7979600.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDRVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(26332..27615)
                     /gene="tig"
                     /locus_tag="ACKB38_000799"
     CDS             complement(26332..27615)
                     /gene="tig"
                     /locus_tag="ACKB38_000799"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGL7979601.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYSKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(28401..29537)
                     /locus_tag="ACKB38_000800"
     CDS             complement(28401..29537)
                     /locus_tag="ACKB38_000800"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGL7979602.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERLENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYVFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(29599..30036)
                     /locus_tag="ACKB38_000801"
     CDS             complement(29599..30036)
                     /locus_tag="ACKB38_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7979603.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(30038..31993)
                     /locus_tag="ACKB38_000802"
     CDS             complement(30038..31993)
                     /locus_tag="ACKB38_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGL7979604.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTGLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESI
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRAYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(32017..33786)
                     /locus_tag="ACKB38_000803"
     CDS             complement(32017..33786)
                     /locus_tag="ACKB38_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGL7979605.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIIATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASSRYDEIVASLTGASPIAEIEGDIPTSAPVDSQESDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            33995..>34104
                     /locus_tag="ACKB38_000804"
     CDS             33995..>34104
                     /locus_tag="ACKB38_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7979606.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGISNSNIR"
BASE COUNT        10156 a         7630 c         6437 g         9881 t
ORIGIN      
        1 taaaataggc tccataatat ctatagggga tttacccact acaaatatta tagagccaac
       61 aataaaaaga aaaagtgttt gatagatatc aaacactttt ttctttgcct cccactatct
      121 aaaaaaatga taatagatat aattgtaaac aaaaatccag ataggttttg catgattgag
      181 aaagttaaaa aaactatggc agagaatcgt taatctcaga ttgtcggtag aacgataaac
      241 aagggcaaaa aagaaaccaa tcagactata atataataaa ctaattggat ctctgtgaga
      301 tagtatcaaa tggctaatcc caaagatgat agcagatagg ataacatcca aatagtactt
      361 ggactaggga aagaaggtat tcataaaata ccctctatca agagtctcct caaaaacagg
      421 accgatgatt acaggcagga caaaagataa gatagtcgat aaaaaggttg gttgtccatt
      481 tgaaaaaagc acggtaaaat actcatcatg aatattccta tgattaatca aatgagcata
      541 gcgtgcccaa aaattaccga gaatctgata aaccacataa gttgcaaata agtagaagac
      601 aaatgaccag ttccagctct ttttctcaaa gataaagagc atctttttct tttttaacct
      661 ccaaattaat agaaggaaac ttcccactaa tcccattgtt aaaataagag aatagacatc
      721 agctcctaac cctaaaatga tcgtcacata caatccaatt gtttgtggta aataggtaga
      781 tagtaaaata ataagcaaaa atattccaaa ttgtcttagt ttttttgtgt ttctcatcgt
      841 acttttttga aagattaccc tgctcggaag ccgtacttcc aagcatctat ataagaatta
      901 agtgcccctt gcctcatata gggagcaaat tctctataat ataaccatct actatatcca
      961 tcttcccaaa cagcaagacc acctgaagtt tgctccaagt cctcagttga aagaactgta
     1021 aatgtatttg tacctgtcat tgcaagtacc ttcttaaaat agattgttgt aggctcacat
     1081 ttatagtata tttctttttt tgtctatttt atagcccatc tcctcaactg gcaatttttc
     1141 gacctgaatt acatttttcc ataaaaaatg agacctttct agtctcattt agtcattctt
     1201 agtattttct aaatcgttga tagcgttctt ccagcaactc ttctagcggt ttttgtgaaa
     1261 gtctagccag ctccgtttgg agttcttttt tgacactctt aatcagttct ttactagaaa
     1321 gtcctatttc agaaatcacc ttatccacca cgtccatttc taacagttca tgcgaagtga
     1381 ttttcatcag ttctgctgct tccatagcgc gagtaccgtc cttccataaa atggaagcaa
     1441 agccttctgg actgagaatg gcatagatag aattttccag catccagaca cggtccgcga
     1501 cagctagagc cagagccccg cctgaaccac cttcaccgat aataatggcg ataataggaa
     1561 ctttcaggtc actcatttcc atgagattgc gagcgatagc ttccccttga ccacgttctt
     1621 ccgctccgac accaggataa gcacctgctg tattgataaa ggtcacaact ggacggccaa
     1681 atttctcagc ctgtttcatc aaccgcagtg cctttcggta gccttctgga tgtggttggc
     1741 caaaattccg tttgaggttg tcttgcaaac tcttgccttt ttggatacca accactgtta
     1801 cagcttggtc tccaagccaa ccaataccac caacaactgc accatcatca cgaaaagaac
     1861 ggtcaccatg taattggata aattcatcaa aaatgcctgt cgcaaagtcc aaggttgtca
     1921 agcgactctg ctcacgcgct tctctgacta tttttgcaat attcatctag gactccctcc
     1981 atgcaatctg actaggctag caatcgtatc tggtaagtct cttcttttga caatagcatc
     2041 cacaaagcca tgttctaata ggaattctgc cttttggaaa tcctcaggca agctttcacg
     2101 aaccgtattt tcaatcacac gacgcccagc aaaaccaacc aagctctgtg gttcagccag
     2161 aatgatatcg ccttccatag cgaaagaagc tgtcacacca ccagtcgttg gatctgtcaa
     2221 aatggtcagg taaaagagac cagcatttga atggcgttta accgccgcag agatcttagc
     2281 catctgcatg agactcatga ttccttcctg catacgggct ccaccagagg ctgtgaatag
     2341 gacaactggc aatttttcga cagtcgcata ctcaaacaaa cgagtgattt tttcacctac
     2401 aaccgtaccc atagaagcca tgataaagtt agaatccata atcccaagag ccacagtctg
     2461 acctttaata agagcagttc ctgtcacaac ggcttcatgc agacctgttt tttcacgcat
     2521 agatgccagt ttcttttggt aaccagggaa atgcaaggga tccttgcttt caatccctgt
     2581 aaacaattct ttgaaggttc ccatatcaat cgtcaaagcc aagcgttctt gggcagaaat
     2641 acgaaaggta tagctacagt gcggacagat acgttcactt cccagatcct tctgatagat
     2701 ggtatgctta cagcctggac actgggaaaa taattcatct ggaacctctg gcttagcttg
     2761 aggtttttcc ctaaccgaac gattgggatt gattcgaata tacttatctt ttttactaaa
     2821 tagagccatt gattcccctt ttcggtttaa actcttaaag tcattttatt ctttttcttg
     2881 atatttaggt aagaaggttt ccatcaagaa ggaagtatca taatccccag caatgacatt
     2941 gcgatctgaa atgaggtcaa gctggaaatc tgcattggtc tgcactcctt caatttctaa
     3001 ttcatagagg gcacgttgca ttttcatcaa ggcgtcaaaa cgattttcgc cgtgtactat
     3061 gattttggca atcatactat cataataagg cggaatggta taacctggat aaactgctga
     3121 atccacgcgc aagccaactc caccacttgg cagatagaga ttagtaatct tacctggact
     3181 tggagcaaag ttaaaggctg ggttttctgc attgatacga cactcgatgg catgaccgcg
     3241 taggacaata tcttcttgct taacagacaa aggctgacct gccgcaatgc aaatctgttc
     3301 cttaacgata tcaacacctg aaacaaactc tgttactgga tgttctacct gaacacgagt
     3361 attcatctcc atgaaataga aattgctact tgcttcatca agaagaaatt caatggttcc
     3421 tgcattctca tagccaacaa actctgccgc tcgaacagca gcagcaccta tttcatgacg
     3481 cagcgttttt ccgattgcaa tcgagggact ttcttccaaa accttttggt tattcctttg
     3541 aagagaacaa tcccgttcac ccaagtgaat cacatgtcca tgctcatcac ctaggatttg
     3601 aacctcaatg tgccgagctg gatagataac ccgttctatg tacatggcac cattgccata
     3661 attggccttg gcctcactag aggcagtttc aaaggcagaa acgaggtcat ctggtttttc
     3721 aaccttacga atccctttac cacctccacc tgctgaagcc ttgagcataa caggatagcc
     3781 aattttttca gcaacaatca aagcttcttc agagttatgc acttctccat ctgaacctgg
     3841 tataacaggc acacctgctt taatcatctg agcacgcgca ttgatcttat cccccatcat
     3901 atccataaca tgaccagatg gaccgataaa cttgatacct acttcttcac acatggtcgc
     3961 aaatttggaa ttttcactga gaaatccaaa accagggtga atagcttctg cctcagtcaa
     4021 gactgcagct gatagaactg cattaatatt gagataagac tctgttgcct tgccaggacc
     4081 aatacaaact gcttcatctg ccaaaagcgt atgaagagct tccttatcag cagttgaata
     4141 aaccgctacc gtcgcaattc ccaattcacg tgccgcacgg ataatacgaa ccgcaatttc
     4201 accacgattg gcaattaaaa tttttcgaaa catggagaac ctccttagtt cccaattgca
     4261 aaagtaaggg taccactggc tgcaagcttg ccatccactt cagcctttgc ttcaaccaca
     4321 gctatggtgc cacgacgttt tacaaaagtc gctgtcataa ccaattggtc gcctggtaca
     4381 acttgcttct tgaacttaac cttgtccata ccagcgtaaa agaccagttt tcctttattt
     4441 tcaggttttg ataactccaa cacaccggca gtttgcgcca aggcttccat aatcacaaca
     4501 cctggcataa ctgggtattg aggaaagtgg ccgttaaaga aaggctcgtt gatggtcaca
     4561 tttttgatag caacaatggt atcctcgctc acttccaaga cacggtccac tagaagcata
     4621 ggataacggt ggggaagagc ttctttgatt ccttgaatat cgatcatttg atacgtacca
     4681 atcctttacc aaactcaacc atttcttcgt tagagacgag aatttccgtt accacaccat
     4741 ccttaggagc tgggatttca ttcatgactt tcatggcttc gataattacc aatgtttgac
     4801 cttttttgac actatcacca actgtaacga aggcaggttt atctggtcca gcagccaagt
     4861 aaaccactcc aacaagtgga ctctctacaa gatttccctc agtagccaca cttgcttcag
     4921 ctggagctgg aacttcttct gctacagtct ctgctggagc agatgtagga gctactggac
     4981 tcggtgttgc tagaacgggt gctggagcga cttgagttgc aacttcaggc acaggtcttg
     5041 cttcattctt gctaaactgc aactcatccg tcccattttt ataagaaaat tctctcaaac
     5101 ttgactggtc aaattgagtc atcaagtctt taatatcgtt taaattcata cttatctatt
     5161 ctcccaacgt ttgaaagcaa gaactgcatt gtggcctcca aaaccaaaag tatttgaaat
     5221 agcgtatgga atttctttct ccaagccttg tccataaacg acattagctt cgatataatc
     5281 tgatacttca cttgtcccag ctgtcattgg tacaaagtta tgacgcatag cttcgatggt
     5341 gacgatagct tctactgcac ccgcagcccc cagcaaatgt cctgtaaaag acttggttga
     5401 tgatacaggt acttccttac caagaacagc tacgatagca ccactttctc ctttttcatt
     5461 ggcaggagtt gacgttccgt gagcattgac ataggctact tgctctggag aaatctcagc
     5521 ttcttccaag gctagtttga tggccttgat agctccctga ccttctggat gtggagaagt
     5581 catgtggtag gcatcacaag tatttccgta accaaccact tcagccagga tagtagctcc
     5641 acgtttttca gcgtgttcaa gactttctag aaccaacatc cctgaacctt cacccataac
     5701 aaacccattg cgatccttat caaatgggat cgaagcacga gttggatcct ctgtagtaga
     5761 gagagctgtt aaggcttgga aaccagcgat ggcaaaaggt gtgatagaag cttctgttcc
     5821 tcccaccaac atcacatctt ggaaaccaaa cttaatggag cggaaggcat ccccaatcgc
     5881 atcatttgat gaagagcagg cagtattgat agatttacaa acaccgtttg caccaaaacg
     5941 catggctaca ttcccagaag ccatatttgg taaagctttt ggaagagtca ttggtttgac
     6001 acgtttgggt cctttttcat gaaggcgaag tacctgatct tcaatttcct tgattccacc
     6061 aataccagat gcaacgataa caccaaaacg atccctatta agagcctcta catcaagatt
     6121 ggcatgattt acagcctctt gggctgcata caaggcatat aaagaatagt tatcaaaacg
     6181 gttggtatct ttttttacaa agtatttatc gaacggaaaa tcttggattt ctgccgcatt
     6241 atgcacatca aagtcactat gatcaaattt tgtaatgcca ccaatgccga ttttcccagt
     6301 tgctaaacta ttccaaaatt cttctggtgt atttccgatt ggagatgtta ctccataacc
     6361 tgttaccact actcgattta gtttcattct tttcacctct agctttcgct acatacttaa
     6421 gccaccatca atggcaacca cttgtccagt tagataatct tggcctgcta aaaatactgt
     6481 caaatctgca acctgctctg cctgcccaaa ttctttcatc ggaatctgag ctagtgtagc
     6541 ttccttaatc ttatctgaca ggatagcggt catatcagac tcaatcattc ctggagcaat
     6601 cacattgact cgtatattcc gactagcgac ctcgcgtgcc acagacttgg taaagccaat
     6661 caagccagcc ttagaagcag cataattagc ttgaccaata ttccccatca aaccaacaac
     6721 actagacata ttaatgatag caccttctct ggctttcatc atcggtttca agactgattg
     6781 tgtcatatta aaggcaccag tcagattgac cttgagcact ttttcaaaat ctgcttctgt
     6841 catcttgagc ataagagtat cttgggtaat ccctgcattg ttgaccaaaa catctactga
     6901 acccagttct gcaatagctt gatcaatcat acgcttagcg tctgcaaaat ctgatacatc
     6961 tcctgaaatg ggaaccacct tgataccata gtttgaaaac tcagcgagca attcttctga
     7021 gattgcccca cgactgttta agacaatgtt ggctcctgct tgagcaaact tgtgggcgat
     7081 ggcaagacca attccacgac tcgaacctgt aataaagata tttttatgtt ctagtttcat
     7141 ttttttcctt tcaaaacttc tacttatttt agtctatttt tctaaaagtg ctactaaact
     7201 cgcttgatct tccacatgag ctaagtgagc agtttgatca atttttttaa caaaacctga
     7261 caagactttc cccggtccaa tctcgataaa gttgcttatg cctgcttctt gcatgacccc
     7321 aatactttca tagaaacgaa cgggttcctt gacctgacgc gtcaagagct gagcaatgtc
     7381 ctctttttgc atcacagcag cttctgtatt gccgactagg ggacaagtaa aatctgaaaa
     7441 acttacctga gctagagttt cagctagttt ctggctagca ggttcaagga gagcggtgtg
     7501 aaagggacct gacaccttaa gaggaatcaa gcgtttggca cctgcttctt gcaaaagttc
     7561 aaccgctcga tcaactgcaa ccacttctcc agcaatgacg atttgtgcag gtgtgttata
     7621 gttggctgga gtaaccactc caagttcaga agctttttga caggcttctt caatgacctc
     7681 tactggcgta ttgagaactg ctaccatctt gccagagtca gcaggagccg cttcttccat
     7741 ataggctcca cgcttagcta ccaaggcaac cgcatcttca aaatccaagg cgccacttgc
     7801 caccaaggca gagtattctc caagagacaa accagcaacc atatcaggct gatagccctt
     7861 ttcttgcaat aaacggtaga tagcaaccga agtcgctaga atggctggtt gcgtatagcg
     7921 ggtctgattg agtttgtctt cttccgtatc gatgagataa cgcaaatcat aaccgagcac
     7981 ctggctcgct cgatcaatcg tttctttaac aatcggatac tgatcataga aatcccgtcc
     8041 catccctaga tactgggcac cttgaccagc aaataaaaag gctgttttag tcatttctta
     8101 caactcctgt ccagcgagag gcttcttctt gaattttctt agcggctccg taatacaaat
     8161 cttttaggat ttcttcagct gtttcttctt tagaaacaag ccctgcgatt tgacctgcca
     8221 taacagagcc accatccaca tcaccgtgaa caactgcttt ggctagagca cctgctccca
     8281 tttgttcaaa gatttctaaa tcaggatctt cttgcttaaa ggcatctttt tcagccagtt
     8341 caaaatctct agtcaactga tttttaatag cacgaacagc atgaccaaag tgctgagctg
     8401 aaatcgtagt atcaatatcc cttgctttta aaattttctc cttgtagttt ggatgggcat
     8461 tcgactcttt tgcaactaca aaccgtgtcc ccacctgtac agcctctgca cctagcataa
     8521 agccagccgc agcaccttca ccatccgcaa ttcctcctgc agcaataaca ggaatagata
     8581 tagctgtggc tacctgtcgc accaaggtca tggttgttaa tttaccgata tgccccccag
     8641 cttccattcc ttctgcaata acagcgtctg caccgatttt ttccatgcgt ttagctaaag
     8701 cgacactagg aacaacagga ataacgatta tcccagcttc atggaaacgt tccatatact
     8761 tgcttggatt tcctgctcct gttgtgacaa ctttaacacc ttcttcaata acgagatcca
     8821 cgatgtcttc cacaaaggga gataagagca tgatgttgac cccaaagggt ttatcagtca
     8881 atgatttgat tttatcaata ttggccttga caacttcttt cggggcattt cccccaccga
     8941 taattcctaa tcctccagcc ttggaaacag cccctgccaa atcaccatca gcaacccagg
     9001 ccatccctcc ttggaaaata ggataatcaa tcttcaataa ttctgtaata cgcgttttca
     9061 tagtgcctcc aaccttcctt gcttacgtaa tagttcgatt tcaccataat ttgacagtca
     9121 aactattacc taaacaagag ggagtgggtt tctccctact ccttctacta atattctgct
     9181 tattttgctt gctcttcaac gtaagcaacc aagtcaccaa ctgttttcaa gtcattttct
     9241 gcttcgattt ggatatcaaa agcatcttcg atttctgaga ttacttggaa caagtccaat
     9301 gaatctgcgt ccaaatcatc aaaagttgat tcaagtgtta cttctgatgc gtcttttcca
     9361 agttcttcaa cgataatttc ttgtactttt tcaaatactg ccatgatagg actcctttaa
     9421 aataaatagt ttttttataa caatgtgttc accacatgat tacctaaatt gtaagaatga
     9481 gcgtgcccca ggtcaagcct ccaccgaagc ctgatagaag aacagtctgg ctaccatcta
     9541 aagggatgag accttgttct acacactctg aaagtaaaat cgggatactg gctgcactgg
     9601 tattgccata ttccatcata ttggctggaa gtttggctcg gtcaacacca atttttctag
     9661 ccatcttatc caaaatacgg tcattggctt gatgaagtag cagataatcc aagtctgtca
     9721 cctctatagg agattcatca atagtctgct tgatagactt ggctacatct cgaatggcaa
     9781 aatcaaagac tgtgcgtcca tccatcttca aaaacgaatc tgcactttct tgatctgaaa
     9841 atggagaatg taaacctgaa tgcccataag ttaaacactc gctgcgactt ccatcgctat
     9901 tgagactctc agctaagaaa tgctcttgct cgctagcttc taacaagaca ccaccagcac
     9961 catctccaaa caacacagct gttgatcgat ccgaccaatc gactgcctta gagagggttt
    10021 cactaccaat caccaagcct ttttgaaagc gaccagaagc gataaacttt tcagcagttg
    10081 aaagagcaaa tacaaatcca ctgcaagccg cggttaagtc aaaagcaaag gctttattag
    10141 caccaatatt agcttgaaca cgagcagctg tagagggcat catcgaatct ggagtaatgg
    10201 tagctaggat gataaaatcc agttcttctc ctgttattcc agcttttgcc atcagtttct
    10261 tagcaacctc tgtagccaaa tcactggtag attctgttct tgaaatatgc ctttgtcgta
    10321 ttcccgttcg acttgaaatc cactcatcat tggtatccat aatctgagcc aagtcgtgat
    10381 ttgtaaccac ttgctctggc acataatgag caacctgact tatttttgca aaagccatta
    10441 tttcaaatcc tccaaaaatt ggtaaagatt agtcaaacct ttacccatga cagcaatttc
    10501 ttcctcgctc atgccatcaa taattttttc taccatggcc ttgtggaagc gtttatgcag
    10561 tctatgaatc aagcgaccct tctttgtcaa atgcagatgc accacacgac gatcctgttc
    10621 tgaccgaact cgctcaatgt agcccttacg ctctaaattg ttcaaacttg tcgtaacagt
    10681 tccaagagtt accatcaact cttttgacac ttgacttgga gtcacgtctg gagccttacc
    10741 gatgacatca atcgtatgca tttctttgat ggagatatcc ttaaaacgac tacctctcaa
    10801 gttcacttcc tcaattacaa ggacattgtt aaatatagat gttaaatatt cattgattcg
    10861 ttggtagtcc atttttcata tccctccttc ttcaaaaaac ttttacaatc aaaacatttg
    10921 acagaaagaa ttatatcaaa attcaaagaa tgatgcaagt attttttatt ttcctgtaaa
    10981 tttaggtctt cttctctccg aatgagctcg cactccttct ttgaaatcct ctgtttgagc
    11041 taaggatttc tgtaggttca gttctaaagt agcgtaaccc tgccagtctt taaattggct
    11101 ctcccaaacc aacttcttaa tggcagcata ggaattactt gaagcacgtc ttaatttttt
    11161 aagaagctgt tctctcgtct tttcaagttt ttcagcttca gaaacgcggt aaaccaaccc
    11221 ccattctagg gctttttctg ccgttaaagc ctctcctgtc atagctaatt gagcagcacg
    11281 cgtcacaccg atactgcggc tcaagagatg aatcccccct gcatctggag ccaaaccaac
    11341 accaacaaag gcttggataa acttagcctt atccgtcgcc aaacagaaat ctgccgcaac
    11401 agccatattc gctgcggcac ctgcaacagc accgtcaact tccattaaaa caggtttggc
    11461 tatttgcttg attttataag aaatcgtatt gaccaactct gcgatttttg tcaatgatgg
    11521 aatatcatcc tcatccactg cccgcttcat ctctaccaaa tctcccccaa ctgagaagac
    11581 ctttccattg gcattgatta agataaaatg cacagctgga ttttcttctg ccaaagtcag
    11641 agcttctaaa atctcctcac acatgggaat atgaaaacca tttgcgacct caggacggtt
    11701 caaggtaagg attgccaagt cctcttcaag ttgataaata atgtgttcca tcagggctcc
    11761 tttttatttt ataagcagtt tatactcttc gaaaatctct ttaaaccgcg tcagcttcca
    11821 tctgcaacct caaaacagta ttttgagctg acttcgtcta gcttcttagt ttgctctttg
    11881 attttcattg agtgttaaat tatttcactg tcaaagtata tcttttttag gagcagaaag
    11941 caagaaaaaa tcgactgaaa gtgtctcagc cgataagaaa ggctcgcttt aggctagaaa
    12001 gcatcttatt ttcttcattg aaaaaagcct ttctttctgc tataatcgta taaaatactt
    12061 actttaggag ttcttatgaa agttgttaaa tttggaggta gttctcttgc ctctgctagt
    12121 caattagaaa aagttttaaa catcgtcaaa agcgattcag agcgtcgttt tgtagtcgtt
    12181 tctgcgcctg gtaaacgcaa tgctgaagat actaaggtta cggatgccct gattaaatac
    12241 taccgcgact atgttgcggg taacgatatt agcaagaacc aaagctggat tatcgaccgc
    12301 tatgctgcta tggttagtga attgggacta aaaccagctg tgctagaaaa aatttctaaa
    12361 agcattcacg ccttggccac tcttcctatt gaagaaaatg aatttctcta cgatactttc
    12421 ctagcagccg gtgaaaataa caatgccaaa ttgattgctg cctactttaa ccaaaatggt
    12481 atcgatgcac gctatatgca ccctagagaa gctgggattg tggtcacaag tgaacctggt
    12541 cacgctcgca tcattccatc aagttatgac aagattgaag aattgacaaa caccaatgaa
    12601 gtccttgtca ttcctggttt ctttggtgtc actaaggaaa atcaaatctg tactttctca
    12661 cgtggaggtt ctgatattac aggttctatc attgctgctg gtgtcaaagc tgacctctat
    12721 gaaaacttta cggacgttga tggtatcttt gcagcccacc ctggtattat ccaccaacca
    12781 cactcgattc ctgagttgac ctaccgtgaa atgcgcgagt tggcctatgc aggcttctca
    12841 gtccttcatg acgaggctct tcttcctgcc taccgtggaa aaattcctct ggttatcaag
    12901 aataccaaca accctgacca tccaggtact cgtatcgttc taaaacacag taatgatgaa
    12961 tttccagttg tgggaattgc tggtgactca ggctttgtca gcattaacat gtcgaaatac
    13021 ctcatgaacc gtgaggttgg atttggccgc aaggttctgc aaatcctgga agaacttaac
    13081 atcggttggg aacatatgcc aacaggtatc gacgatcttt ctatcattct ccgttctcgc
    13141 caactaactc ccatcaagga agaagaaatc ctgcgtcagt tggttcaaaa ggctaaagta
    13201 gatcatgcag aaatcgaaca cgacctttct atcattatga ttgttggtga aaaaatgaag
    13261 agccatatcg gagtgactgc tactgcgaca cgcgctctat ctgaaaacaa aatcaacatc
    13321 cagatgatgt ctcaaggttc tagtgaagtt tctattatgt ttgttgtcaa taaagaccaa
    13381 gagaaagcag caattaaagc cctctacaat gccttttttg gagaaagtaa ggaagactaa
    13441 gcatggccca atcacttaac aaaacagtac tcctcagtac gacaggcact tcctacctct
    13501 ctatcgctgg gaaagttggg aaattccttg tcggagatca ggctttggaa ttttacccag
    13561 atgtcaatgt cgaacaattt atccagattc cttggagcca tatcaatcag attggagcca
    13621 atgtcactgg acgcaaaatc agccgccact tcgaagtctt tacagacaaa ggaaaattcc
    13681 tctttgcttc aaaagactca ggtgccattc tcaaaattgc acgcgaaaaa ctaggcaatg
    13741 acaaggtcgt caaacttccg accctgattc agacaattag ccaaaaattt aaaaatctat
    13801 ttgcaaaaaa gtagaaactt tagtataatc atagcaacgg ataagtaggt tatcttcttc
    13861 tttcagaaag tctgcgggta ctgcgagcag ataggaggga taggtgaaat tctaccgtta
    13921 gtgacaatta catacacaat caagtgcaca cctgtgaagt tggatggaac cgtggccctg
    13981 ccactccaac gttttgtcag ggtgcttttt tcataaagga gttcttatgt tagatatcaa
    14041 acgtattcgt acagattttg aagctgtcgc agaaaaatta gctacacgtg gtgtagatgc
    14101 tgctgtcttg aatgaaatga aagaaatcga tgctaaacgt cgtaacatct tggtcaaggt
    14161 tgaaactctc aaagcagaac gtaacacagt ttctgctgag attgcccaag ctaagcgcaa
    14221 caaggaaaat acagatgaca agattgctgc catgcaaaat ctatctgctg aggttaaagc
    14281 cttggatgct gaattggcag aaatcgatgc taaattgaca gaatttacaa cgactcttcc
    14341 aaatatccca gctgacagcg ttcctgttgg ggctgacgaa gacgacaatg tggaagttcg
    14401 ccgttggggt actccacgcg agtttgactt cgaacctaaa gctcactggg atctcggtga
    14461 agaccttggt atccttgact gggaacgcgg tggtaaggta acaggcgctc gcttcctctt
    14521 ctataaaggc ctcggtgctc gtttggaacg tgctatctac aactttatgt tggatgaaca
    14581 tggaaaagaa ggctatactg aagtcatcac accttacata gtcaaccatg attctatgtt
    14641 tggtactggt cagtatccaa aatttaagga agatactttt gaactcagcg ataccaactt
    14701 tgtcttgatt ccaactgctg aagttcctct gacaaactac taccgtgatg aaatcttaga
    14761 cggcaaagat cttccaatct acttcactgc catgagtccg tcattccgtt ctgaggctgg
    14821 ttctgccggt cgtgatacgc gtggcttgat ccgtttgcac caattccaca aggttgaaat
    14881 ggtcaaattt gccaaaccag aagaatctta cgaagaattg gaaaaaatga cagccaacgc
    14941 tgaaaacatt cttcaaaaac tcaaccttcc ataccgtgtc gttgctctct ctactggaga
    15001 tatgggcttc tcagctgcga agacttacga cttggaagtg tggattccag cacaaaacaa
    15061 ttaccgtgaa atctcaagct gttcaaacac agaagatttc caagcccgtc gtgcccaaat
    15121 ccgttaccgt gatgaagcag atggcaaggt gaaactcctt cataccttga acggttctgg
    15181 acttgcagtt ggacgtacag tggctgcaat tcttgaaaat taccaaaatg aagatggttc
    15241 tgtgaccatc ccagaagcac ttcgtccata catgggtgga gctgaagtca tcaaaccata
    15301 aaaaataagg tttagctatt tctagctaga ccttttttcg taaccaaatc agataagcac
    15361 ctagtacaaa gaataaaata gttaggcata taatggtttc agccaatacc aggtaatcca
    15421 gaaatggaag tttcaaaatt ccctgagcca tcttgagcga ggtcgctgtg ataatggttg
    15481 ggaaggtgag ggctgagaag gctggttgaa aaccttgttt taaaatgttg ggcagacgag
    15541 ttaaaacaaa gaaaaagaag gattgagaag ccaaaatcat gacaatcaag acccaagtcg
    15601 gcaggctggt tcctcctact cgaactagag aagccaagag tagagagaaa ggagcacagt
    15661 agattccttc ttgtccaagc aaggctagtg ggagtggatg tttctttaaa tcgctataaa
    15721 taaggggata gagatagaag gtcaagagaa aaccaaaact caaggtcgca taggcaattt
    15781 cgataatacc taccagagga taggtcaagg cagccactgc tatccccaca tagagaaccg
    15841 tccagcttgg agtggcatga accctccgcc ctggacaagc aaacttgatg gtaaaaccag
    15901 caatcaaggt caaatccaag agaaatgaaa accaccaaat cccttgtgct accaaaggaa
    15961 gataagagaa tacgcgaaag acataggtcg ataaaatcat cccagccata ggaaaggttg
    16021 ccattcctga caaaagaggg ggcttggtca attcttgctt ggtttctttc caattaaaga
    16081 gatgcagaat tagaaagtaa atccataaaa ccaaaccaat cagactaaaa agatgggata
    16141 gaaccggcaa cgtatctaaa ataagatttc cagctcctgc caaacctagc aaacaacctg
    16201 aaaatactaa ggggagtttt ttcatcctaa cctccaataa tcatgttagt ttcagtataa
    16261 cataaaagcg cttaaatgag gatttaaaaa aacgaagtcc gcttatttca gacttcattt
    16321 tactcagata tgaattaggc ataaggttgc aattctggat taattggtgt attagctaag
    16381 ttgttggcat agttacagag gattgctagg ctgacaccaa aaaccacatc caaggcattt
    16441 tgttgagtgt agccagcttc taaaaactca gacaaggctt catctcctac acgacccttg
    16501 gtattgataa ctgccaaggt aaacttagct agggtatcca atttaggatc tgtttcaatt
    16561 ggagtacgat tgcgaagagc ttgaatcaag tcatcattca tctggatttg tttgatggaa
    16621 aaggctgtgt gacctgcgac acagaaggca caaccattgg tcacggctgc cgtgatttgc
    16681 accacttcac gctcaacggg tgtcaggctg ttgcgacggt ggatagatga gacaatttgg
    16741 taggcttcta aaacagtcgg ggcattggcc aagagaccga ttaggttggg aatatagcca
    16801 ttgttgtctt tttctactgt ttcaagaatt tctttcactt ctgctggtgc tgactctact
    16861 gtatggatag taaatgttgt cataagatac ctcttttctt attattgaca ctaatattat
    16921 tggaaaatct tataaaatcc tgattcctaa gtttatctaa gataaagctt tattctctca
    16981 taagattttc gttgttatat tagtttatca cacttccaat cacttgtata atatatatta
    17041 tatatcaggc tgataaaaat tatttatagg caaaaaaatc acacgagctg tgtgattcca
    17101 ttatttgtca aaatactttt tagtttcagc aataacgact ggcgacaaga ccaagagggc
    17161 aatcaagttt ggcagagcca tcaaggcgtt aacgatatct gcgataatcc agaccatatc
    17221 caactcgata aatcctccta acaagaccat gagcacaaaa accacacggt agagccagat
    17281 aaagcgaacc ccaaagagga actcaaaaca gcgttctccg taatagttcc aacctagaat
    17341 cgttgtaaag gcaaaaagta caaggaagat ggtcaagaga gcaggcccaa agtgtgaaaa
    17401 gtttgttgag aaagctgact gagtcaaggc aaccccattc aagtcaccgc tccaaactcc
    17461 agttaccaag atggtcaaac cagttagagt acaaatgatg agggtatcaa taaaggttcc
    17521 tgtcatggaa atcaaacctt gctctactgg ttcatttgtc ttggcagctg cagctgcaat
    17581 aggagcagaa cccagaccag attcgtttga gaacacacca cgcgccacac cattttgaat
    17641 agccatccga acgctagcac cagcaaatcc acctaccgca gcaaggggac taaaagctga
    17701 ggtaaagact aaagcgattg tgccagggat ttttccgata ttaaagaaaa taactgtaag
    17761 agttcctaag atataaatga tggccataaa aggaacaaca gtagttgaaa ccttagaaat
    17821 agacttgagt ccaccaaaga ctgcaatcgc tacaaagaca gacaagacga gagctgtgat
    17881 ggctggcgaa atcgtcgttg tattttggat agattctgca atcgagttga cttgggtgaa
    17941 ggttccgatt cccaagagag caaccaatac tcctgctact gcaaacaaaa cagcaagtgg
    18001 tcgccacttt tctcccatcc ctagaaggat ataatgcatg ggacctcccg ctactgcacc
    18061 atggtcgtcc ttggtgcggt atttgatggc caagagtcct tccgcatact tggtagccat
    18121 tccaaagaaa gccgccatcc acatccaaaa tagagctcct ggtccaccaa ccttgatagc
    18181 cgtcgcaact cctatgatat ttcctgttcc aacagttgat gccaaggctg tacacagagc
    18241 tgcaaaactg gatacatcac catgtccctt atcctggata aaaataagct gaaaggcctt
    18301 gggtagacgc aaaacctgca agagtcctag ccgaatagtt aggtaaatcc ctgttccgac
    18361 caataaaatc aagaggggcg gtccccaagc aaaagcatcg attgatttaa gcaattctaa
    18421 catttccttc tcctatcgtt tcaaccccaa aagaaagagc acatgcaaga tacatgtact
    18481 ctggaatgct tagataaatg ctaaaaagcg gtctatccta gctctgtcct tttacctgag
    18541 agtttgagca gttgcctgcc ttgccccttc ggtgccttta cggtctctcc agagttccgt
    18601 ccatttacag tcatggaaaa tcaaacgatt ccccacttct attaaacttc attcggtgtt
    18661 ggtatttaat tgattctaat tttacaaaaa atgttggctt ttgtcaatgt gtttattagt
    18721 aaaaattagt tcaacagttt ttactttata aagtccagaa tactgctatc ctttaaaagt
    18781 gacaatagtc gcaccactgc ctccagcatt ttgtggggca tagccgaaac tcttgacatg
    18841 tttgtttctt tgcaagtatt tggtaactcc ttcacggatg actcctgttc cgataccatg
    18901 gatgatatca acttgagcca tattgttaag caaggcttgg tcgatgaagg tatctagctc
    18961 attcatggct tcttcatagc gcttgcctcg aagatccagt ctagcttgag gtcctcgccc
    19021 agaagttcgt ttcacaacat tgacctgttt cttcttgact ggtttttctt gctgggcttg
    19081 aacaagatca aactctttct cttccaaggt catcttaatc aagccaactt gggcttccca
    19141 gcgaccgtcc ttgagttgac tggtcaaggt accacgctga ccataactga gaaccacgat
    19201 atcatctccc acctttggag ctcgtttttt cttggccttt tgaaggacct tatttttaga
    19261 caagtccact ttttcaggag ccaatttttt caacttggcc ttggcttcaa tgatttcgtg
    19321 gggcttgagt tgggatttac tgtggagatt tttgagaatc tggtcacttt cacttagggc
    19381 catatccaca atctcagcag cctgttcacg cgccttgtta agctcggttt ccttttcacg
    19441 attaagctcg ttgtagagtt tttttagcgc acggttcatc ttgagatttt cttgctccac
    19501 ctcacggata ttgtccaaac gtttgcggct ttccagcgtc tgctcttcta attgctcaat
    19561 gatacgattg acgtcattgt cctgatcgat ctgctgactg gcatctccta cgataacttc
    19621 agataggcct agacgtttgg caatttcaaa ggcattactt cggccaggaa caccctgcat
    19681 aaagcgatag gtcgggcgaa gagttgcagt atcaaactcc atactggcat tttgcacaaa
    19741 ggctgtctca ataccgtagg ccttgagttc tggatagtgg gtcgtcgcca tggtcttgat
    19801 ttgacgcagg cgaaggtcct ccagaatagc catggcaagg gctgctccct cttggggatc
    19861 agtaccagcc cccaactcat ccaaaagtaa gagtgaatgt tggttgacct tgccaagaat
    19921 atccacgata ttggtcatat gactagagaa ggtagacaag ctctgctcaa tagactgctc
    19981 atctccaata tcagcaaaga tttcttcaaa aataccaaca cgacttccct tgtctgctaa
    20041 aatcggcaat cctgactggg ccatgacctg tgtcaagccc agagttttga gcatgatggt
    20101 cttcccacct gtattgggac ctgtaatgac aatagctgtt aaatcttgac caaaatagac
    20161 atcatttgcg acggcatttt tgaccaaagg atggcagaca tggagcagtt gaatctcttg
    20221 attttctgac agctgaggca cgactgcttg tctttcttgg ataaatcgaa ccttggcacg
    20281 aatcaagtcc agatgaccga taatccaagc gtcattagca atctcagccg catgagggcg
    20341 gacacgctca gaaatttctt ggagaatgcg aagcatttca tagcgctcat ctgctcgcag
    20401 actagcaatt tcttcgctca gtttgactac ctcacggggt tcgatataga cggtgtttcc
    20461 actagcagaa atatcatgaa cgacacctgc aatcttattg cggtaggtgt ttttgactgg
    20521 taaaacctga cggccatttc tgctagcaac aattccttcc gtcaacagct gcgctttttg
    20581 cttgagcaag tcttgtaaaa catcgcgtac ctgactctcg ctatcatgta tttttcgacg
    20641 gattcgcgcc aattcttcac tggcaaaatt ttcaatgaaa cccgcatcat taaaggcctg
    20701 aagatttcct tgtaattgcg gaaaatcatg taatttctca aaccaaaggg ctaattcttc
    20761 caagctgaca ttttccagat tggtgtaaaa attttgaagt tctcggctgg caagaagcac
    20821 gcgtttcaag agtaggaact cctcgatatt gagatccgct cccatctcca acctcttgca
    20881 gactcctgca atttccttag ttgagagaat agtaaaatgc ggttgctcga cgaaaagagc
    20941 ctgcatttcc ttcatctcag caaaagcctg tttgatttta tctgctttgg cagtcggagc
    21001 cagttgtctc aattgctcca agccctgctc ggtcaacaaa tgaggctcaa acaaggcctt
    21061 gaccttatcg aactctaatg tttctaatat tttcttattc atctttattt ccttgtcttt
    21121 gaatgtctag gtgtggtagc tttttacatt ctgatagatt cgagtcaaat ctgtaaacaa
    21181 agggctagga aaactcctgc cctttttatc cgattaaatt tgtcacccag atttgtttga
    21241 gccaactggt tgttaccggt atgctctgga tgatgtgttt tgcgacgata ctcttttcaa
    21301 gaggattttg tataactgcc atggggatgg tcgccaagat tgtcaaggcc atttgcaaga
    21361 caaataaggt caccaacatg gacaagatac ctgctgaaac ttggaacaac ttaccaccca
    21421 gttttttgct aggaatcaag tgtaagagaa gaccaagtaa acgaccaatg ctatagacaa
    21481 tcccaaatac aagcaagtag ccgatacctg cataaaagac cttatccagc tgaaagagtt
    21541 gatccgatgg gaaaaagaaa gtcccctgac cttcctgcga atttgcataa gggagcaata
    21601 aatggaattg ctctccaagc cccttataaa actggccagc cataaaagcc gatgccatgg
    21661 ctgaaatcag gtaataaacc tgtaagagca ggcctctccg atagccgata taaaatcccc
    21721 aaaccaagac caatagaaga aggaatgaaa tcataaggaa tcctcaatct tgctctgttc
    21781 ttgcttacaa gtcacaagct tgtgacggag ttcttctagc tcttgctcct tatcgtcaaa
    21841 ttcaatctca cggctgagct gagttgataa acagttgact gccaacaaaa gagcgattgt
    21901 ttcatcatct gcgctaggca tttgttcttt aattgcttgg tatttttctg tcgcaacctt
    21961 agcgatttcc tccataaaaa ggttgtcatg ttcgcttgtc aaggttaacg attttttccc
    22021 gaatgtaaat ttgaatcgat ttagatttgc cataaaattc acctcacgat attataccaa
    22081 aattcgctaa ttttgtcagt ttttacaaat tttactgctt ttgtggtaca atagaaacta
    22141 tggcaagtat cacactcaca ccaagcgaaa aggatattca ggcttttctt gaacactatc
    22201 aaaccagtct ggctcctagc aagaatccct atatccgcta ctttttgaaa ctacctcaag
    22261 caacggtttc tatctatact tctggaaaaa tcttgcttca gggtgaaggg gctgaaaaat
    22321 acgccagttt ctttggctat caagctgtag agcaaaccag cggacaaaat cttcctttaa
    22381 ttgggacaga tgaggtggga aatggttcct actttggtgg gcttgcagtt gtggctgcct
    22441 ttgtcacacc tgaccagcac gactttttac gaaaactcgg tgtgggggat tctaagactc
    22501 tgaccgacca aaagatccgt cagattactc ctattctcaa ggaaaaaatt cagcaccagg
    22561 cactccttct ctcacccagc aagtacaacg aggtcatcgg agaccgctac aacgctgttt
    22621 cggttaaggt tgccctccat aatcaggcta tctatctcct ccttcaaaaa ggtgttcagc
    22681 ctgagaaaat tgtgattgat gcctttacca gtgctaaaaa ttatgacaag tacttggcac
    22741 aagaggccaa tcgtttcagc aatcctatca gcttagaaga aaaggctgag ggcaaatact
    22801 tgtctgtcgc agtttcttct gtcattgcgc gtgatctctt tctggaaaat cttgaaaatt
    22861 tgggacgaga actgggttat cagctcccaa gtggagctgg aacggcttct gacaaggtgg
    22921 ctagccagat tttgcaagcc tatggtatgc agggactcag cttctgcgcc aaattgcact
    22981 ttaaaaacac tgaaaaagcg aaaaaacgct tagaaaggta agttatgaat tcatttaaaa
    23041 atttcttaaa agagtgggga ttgttcctcc taattctgtc attactagct ttaagtcgta
    23101 tctttttttg gagcaatgtt cgcgtagaag gacattccat ggatccgacc ctagcggatg
    23161 gcgaaattct cttcgttgta aaacaccttc ctattgaccg ttttgatatc gtggtggccc
    23221 atgaggaaga tggcaataag gacatcgtca agcgcgtgat tggaatgcct ggcgacacca
    23281 ttcgttacga aaatgataaa ctctacatca atgacaaaga aacggacgag ccttatctag
    23341 cagactatat caaacgcttc aaggatgaca aactccaaag cacttactca ggcaagggct
    23401 ttgaaggaaa taaaggaact ttctttagaa gtatcgctca aaaagctcaa gccttcacag
    23461 ttgatgtcaa ctacaacacc aactttagct ttactgttcc agaaggagaa taccttctcc
    23521 tcggagatga ccgcttggtt tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag
    23581 atatcacagg ggaagctaaa ttccgcttat ggccaatcac ccgtatcgga acattttaag
    23641 aaacctaaga ggcagagaat caccaatctc agcctcttct tctatcgtga gaagtgattg
    23701 tttatttaca aatactcaat gaaaatcaaa gaacaaacta ggaagctagc cgtaggctgc
    23761 ttaaaacagt gttttgaggt tgtggataga actgacgaag tcagctcaaa atactgtttt
    23821 gaggttgcag atggaagctg acgtggtttg aagatatttt cgaagagtat aaaataaaaa
    23881 caatcccaac tctcacctat tcatgcaaag gaattttatg gaagtttatt tttcaggaac
    23941 tattgaacgg attatttttg aaaatcccag caatttttat cgcatcctcc tcctagaaat
    24001 cgacgatacg gacgcagagg attttgatga ttttgaaatc attgtcacag gaaccatggc
    24061 tgatgtaatt gagggcgaag actatacttt ttgggggcaa attgtccagc actccaagta
    24121 tggagaacaa ctgcaaatca gtcgttatga tcgcgcaaaa ccaactagta agggcttggt
    24181 caagtacttt tcaagtagcc atttcaaggg gattggtctc aagacagctc agaaaatcgt
    24241 ggatacctat ggcgaaaata ccattgacga aattttgcaa cacccagaaa agttagaagg
    24301 catcgcagga ctctctgcca aaaatcgcga ggctttcgtc tccactctcc gtctcaacta
    24361 cggaacggag atgattttgg ccaaactagc caactacggc attcccaaca aactagcctt
    24421 tcagattcaa gacttttaca aggaagaaac ccttgatgtg gttgaaaatt atccctacca
    24481 gctggttgag gatatcaagg gtttgggctt taccattgct gaccaactag ctgaggaact
    24541 aggcatcgaa agtcaggctc ctgaacgctt ccgcgccggt ctagttcaca gtctttttca
    24601 ggcctgtatg gaaacagggg acacctatgt tgaagcacgg gatttgctgg aacaaaccct
    24661 tactctcctt gagtcttccc gtcccgtgga actagacccc agccaagttg cccaagagct
    24721 ctcctacctg atcgaagaag acaaggttca gcagattgat accaagatct ttgacaacag
    24781 cctctttttc gctgaggaag gcatccgcag tcacttggtt cgtatccttg aaaaaggaaa
    24841 acagaagagt catgatttag aaaccattca aaaacatatc actactgtcg agcaagaact
    24901 gggaattgag tatgataaca ttcaaaaaca ggctatctgt gatgctatcc agaataaggt
    24961 ctttatcctg acaggtgggc ctggtactgg taagacaact gttatcaatg gaatcattgc
    25021 tgtttatgcc cttttagaag gacttgactt caggaagaaa agcaatctgc ccattcttct
    25081 tgctgctcca actggtcgag ctgctcgtcg catgaatgaa ttgacaggtt tgcctagcgc
    25141 gaccatacat cgccacttgg gaatgacagg tgacgatgat accagtcatc tggaagatta
    25201 cctagatgct gactttatca tcgtggatga attctctatg gtagatactt ggctggccaa
    25261 tcaactcttc tccaatatct cttctaacag taagatcctc atcgtaggtg acagtgatca
    25321 gctaccttct gtcagtcctg gacaggttct agcggatcta cttcatattc ctttgattcc
    25381 tcagactcgc ttggaaaaaa tttatcgaca aagcaaagaa tcaaccatcg tcaccctagc
    25441 tagtcagatt cgacagggca tcttgccagc tgatttcacc caaaaaaaag ctgaccgttc
    25501 ctactttgaa attgctagtg gccatattcc tgccaccatt gaaaaaatct taggtgccgc
    25561 cctcagaaat ggtattcccg cccgtgatat ccaagttctg gctcctatgt accgagggac
    25621 ggcagggatt gatgctatca atcagctcat gcaagacctg ctcaatcccc cacaaaaaga
    25681 tcaactcagt tttgaagctc ctcagtgtca ctatcgtaag agagacaggg tcattcattt
    25741 ggttaacgat gctgaaatca atgtctttaa tggggattta ggagctatca cagacctgat
    25801 tcctggtaaa tacaccgaat cgaaacaaga cgagattgtc attgattttg atggtaatga
    25861 agtctcttac ccccgtaacg aatggtacaa gattcgcttg gcctatgcca tgagtattca
    25921 taagtctcag ggaagtgagt tcccagttgt catcctaccg attactagtg ctagccgacg
    25981 tatgctggag cgaaatctca tctacacagc cattacacgt gccaaaagca aactcatctt
    26041 actaggcgaa ttacaagcct tcgactatgc tacccaacac atcggaactg cccgaaaaac
    26101 ctatctgatt gaacgcttca gtgatctact ggagaatgtt gaagaaaagc aacaagctgt
    26161 ctctgaaaca gtcacatcaa gtgcctctga acaatcctac atcctaaccg aagaaaactg
    26221 ggaccgcatc ccagccatga ttgggattac agacacagac ctcaaagaga tttttggaaa
    26281 atagtgcatc agaaaaccca ccaattcagg tgggttttct gtttattaag attattttac
    26341 tgttgctgtg cttgtgatca attcaacagc ttttttgata gtgatatcat gtttcaacat
    26401 gtcagctgaa agcaagtttt gaacttgtgc aacttccatg ttgtagtctg ctgccaattg
    26461 ctcaacttct ttttggattt cttcttctga agcatcaaat ccttcagctt tggcaactgc
    26521 ttcgataaca aggttagtct tagtacgtga ctcagcttct gcttggtatt ggttgtgaag
    26581 gtcttcttga gtagttccag tgatttggaa gtacatgtca gggttgatcc cttgacgttg
    26641 caaattccca aggaattcat ttactgaacg gtgaacttct tcatggatca tttcttctgg
    26701 aagttctacg atttcagcat tttctacagc tgtatcaatt gctgcacctt caactgcatc
    26761 tttgtaagct tcttctttag cagcagccaa ttctttgctg tatttttctt tcaagtcagc
    26821 aagtgtttca acttcttcat caatgtcttt tgcaagttca tcgtcaagag ccggaacttc
    26881 tttagctttt acttcgtgga tagttgtcac gaatttagct tctttacctg caaggtcttc
    26941 tgcttggtag tcttctggga atgttacgat aacatcaacg gtttcgccag ctgagtgacc
    27001 taccaattgg tcttcgaaac cagggatgaa ttgacctgaa ccaagtccaa gtgagaagtt
    27061 ttcacctttt ccaccgtcaa attcaacacc gtcgatagaa ccaacgaagt cgatcacaac
    27121 agtgtcgccg ttttcagcag cagcttcctt gataaccaat tcagccaggt tgttgcgttc
    27181 gcgttcgata cgctcttcga catcagcgtc agttacttct ttttctacat caactgatac
    27241 ttcaaggttt ttgtagtcac ccaattttac ttcaggtttt gtaacgactt cagcagtgat
    27301 aacccagtct tgaccttttt ccattgaagt tacgtcaatt tttggttggg caaccacttc
    27361 aagaccagct tcttttacag ctgcttcata agcgtttggc aaaagtgcgt tcattgcatc
    27421 ttgataaaga gcttcttcac caaatttttg gtcgaagata gggcgtggaa ggtgaccttt
    27481 acggaaacct ggaacattaa gagatttctt cactgacttg aagacacggt ccaattctgg
    27541 tttgatttgg tcttgagaga tagtgaaagt caagacacca cggtttgttt ctttgttttc
    27601 aaatgataca gacattctgt catttctcct taaaattttt taaatacagt ctattataac
    27661 attttctata aataaaatct agactaatgc agacaattat agacaacaag cctttttcct
    27721 aactaaaaca ctcataaagt atagacaatt ttagactact ttggatatct tttggttagt
    27781 cattttccaa aactaattta tctaattttt cagttatata gtagattgaa actagaatag
    27841 tacacctcta cttctaaaac atttttagaa atcgatttga ctgtcctgat cgatttgtcc
    27901 tgttcttgtt tcattttact atatagatcg cattcgaaag aattcgttcc aaaattgctt
    27961 ggttcgttct tctcttggac gactaaagaa ttgatgggca ttattttttt caacaatttt
    28021 cccactgtcc ataaaaataa catgatgggc tacatctcta gcaagaccta tctcataaag
    28081 ctgatcaaaa actcaatctg aggagaaaat ccaataaggc tttccataat aaaggacatc
    28141 ctatcaagat ttttgaacac ccgataggct gccttttccc gattttgaag actttttgtc
    28201 cagcttcttt taccttataa ataacaacaa aaatcaaaga caagctagaa agattaccgc
    28261 agataactca aacagtgttt tgagattgca gataagactg acggagtcag ctcaaagcac
    28321 tgctttgagg ttgtagatag aacaacatat gcctaagaca aagcgatgct aacctgattt
    28381 gaagagattt tcgaaaaaga ttaaatatac tctacaattt gctcaatcaa ttcttggtcg
    28441 tctaaacctg taacttgtat aacaacatct ttgactgatt gtttagccaa caattcacct
    28501 aatcgaatac tttcttcatc atttacatcg cgatagtcaa agacatagcc aactgtttta
    28561 agtaggtttt tatatgacaa actgagttct ttcaattcac gtatcggccg gatgaatcgt
    28621 tcattatagc ctaattttcg gattggagta cgagctacgc gactaacctc gtccactatg
    28681 aaagggtttt caagtcgttc tataatgact ttgtgataat tctccaattc ttttttatca
    28741 aagttccatt tggcaatcaa gagactccga atttcagcta atacagattc aatccgagat
    28801 ttaatattag gattttgaag agcttccaaa attgtcttgg caccataatg cgcaccaatg
    28861 taagctgaag ttgcatgtcc agaattgact gaaaaaagtt ttcgctcaat aaagggttct
    28921 aaatcttctt cataatgcac atcttttaga cgtaaatctg gatttttaag acgcttggtt
    28981 tccacgaccc attcattaaa gggctcgacc acaacaaaaa gggaatcttc gtgactttgt
    29041 gctggaacaa tcctgtctac tgcagcattt ggaaaaccta tgtagttatc agcaaatgtc
    29101 aaaccttccg gacttaaata tttcttgact tcttgataaa gaaattgaga cccgccaatc
    29161 atattttcac aggccataac atccaatgcc tgtgtatttc ctgcaactcg gcgagcttcg
    29221 attcctttgg ctagaagttc ggcgataaaa gggagtatat taggtccgat tgcagtagta
    29281 ataatatccg tcttttgaat cgcttcaatg acttgctcag gatgttcttt gctattaatg
    29341 ccagccacat tagttacttc tatacgagac tgtcctttct gtgcaatttc aatttcatac
    29401 ttgccctttt cattcagagc atgaattatc tgattattga catccacaaa atcaatatgg
    29461 aaaccatttt taaatagaat ttcacctata aaaccacgac cgatattacc ggcaccaaaa
    29521 tgaacagaat gtttcattca atttctcctt ttcctatttg agggacatat atactagatg
    29581 acaaatgact aaattcaatt attcaacagc atcaaataag cgcaatactt cctcttttga
    29641 ctgagcatct gctagtttaa gaacattatc tacatctgca cagaagatag aaattttctg
    29701 aataatttct aagtgttcat taccaatacc agcaatacca aaaagaaccg ttgccacttg
    29761 agggttagat acattcccaa aatcaacccc tctagggact tgaacgactg taataccaga
    29821 cttgaggaca tcattttttg ctgcatctgt tccatgcggt atggcgataa agttacccat
    29881 gtaaacagat agctctttat ctcgctcaat catggcttca atatagtctt cattaacata
    29941 tccaccctca taaagaactt gcccacaata acaaatagct tcctccttgt tagaaaattg
    30001 tttattaagc ttaatcaaat gtttttcaag tttcatatta gttctccaat ttcttaattt
    30061 tttcgttaaa aatagtattt agcatctgat aaataatgga ttgatttccc gtcttgtaaa
    30121 tctctgtata aagatgattt tcaataatcg actgactaat agctgtcatt aaatctctag
    30181 cttcctcgct agcttcttta gacattagca ttagcagaca ccttttgacc tcttcttcct
    30241 ctcgtttcat tgacaatgcg gagataggta gtttgagttc aaacatagta aaactatttg
    30301 ttgttacttt gctagactga ctatgtaaaa gcaccagacc agtattagga atagccatag
    30361 gactattctg gaagtgagat aacaattttc ttgttaggta agctctgtca ctcacattct
    30421 tctgatattg gatgatttgt tctaccgtgt gctcaaatga ttgattattc tccaattgaa
    30481 ccaactcgaa ttgactcaaa agctgactac tagatattaa ataatcttgc aagtcataac
    30541 ttttctctgg agcgatagca tcacgcgcga caattgtacg atgtgcctgt acatactgaa
    30601 gtcgttcctg taattcaagc aattctttga cggttggaaa actagaaatt acatcgattt
    30661 cctgattagt cagcggcttg gtagataaaa tataatcata ctgactgaga tcaatactgt
    30721 ggtaatctac tagagattga atatctatca actctacaaa aggagcaaca ctcttaattt
    30781 tactgactaa taaatctcta gtgacccgac gttcatccgt taaaagcaaa acacggactg
    30841 gataaatctc tggactacgg cctaagctag agataaaatg tagggcaatc atgccatact
    30901 catactctgt atgaagatat ttcggaaaaa tatcattcac taaaagactg atgactgtat
    30961 aaagaaattt attcctttca actaaaatct ggatagattc tggcaaattt tcaccctgaa
    31021 aaaggatagg gacgcctaaa ctgagccgaa tatgatggaa aagaaaattg aataaaacct
    31081 tgtccttaaa aaagtcaatc ttggtataca tggaaaccgt atcaatcaga tttgaaatat
    31141 tgtagaaaaa ttcaccatca aatttctccg taaagagcgg attgtcctga cgtttaatga
    31201 ttaattcatc caagatgctc gcaaaataga taatttcctg aatactataa aattgtgcag
    31261 tctgcttaga gtaagcttga aaaatttttt gagaaatttc caaagcctgc ttactagtat
    31321 taggtgaatt ttcaatgttt tgctcctgac ctataagaga taacaagatc gcaaaaaaca
    31381 tcttcatcct agcatccata tctggaaaac ctgacagttg cttattaaca atctgactgg
    31441 ccagcccagt tctatctgct tctaaaatat caaagctccc aaaattaccg gttgaaaaat
    31501 ctgctactga gatacagttt gtcagtaaaa tagccaaaaa tcttctctta ccaactgaat
    31561 caccagaaat ccgataacct ttttgtcgtt caattttcag atcaaagtct aaaagacgct
    31621 tatcaatatc tgaaatatcc tgaataatag ttacattact gactttcgtg cactcttgca
    31681 atgcttcatt ggtgacaaaa ccactctcag tcagaaggcg ataggtaatc aactcttgtc
    31741 tttcttgggg actatactca actaacactt caagttctgt cggcaaatca tccaactctc
    31801 cagtcaaaat atagtatttc ccatgcttcg ttatttcgat tccatattgc tccatgctat
    31861 ctgtcaaatc tgataaagtt cgataaattg ttctagatga aacctgtaag atttcagtca
    31921 tatcttgcat tgaaagcttc cctacatgta ggaaagcctt caataattgt tcttctcgtt
    31981 tggttaatag cattcgtaat ttagaaacct ctagttctat ttgtacattc tagcagccat
    32041 tttgtcatat tctggtgttg ttactaaact atccacaata agaaattgag aattcggtgc
    32101 tgcttgctgc acttctgcct gtaaagaaat agttgttaca atcattatgt ttttagaatt
    32161 aaattcacct aattctgaaa ttttggcagt agaaactgga atacgaatat tcttgtttct
    32221 aaaaatagcc cgaatcgttt cacagcccat agttgcagtt ccctgtgctt taccataagc
    32281 aactactaca gcatcaatat ggttaaggtc actttcctga ctatctactg gtgctgaagt
    32341 tggtatatct ccttcaattt ctgctattgg agaagctcct gttaatgaag ctacaatttc
    32401 atcataacga gaggacgcta agaaattatc aacagaaaca tgaatagcac ttggactctt
    32461 gtctttagct cttggtgtca gttcttcctg agtaacaatt aatgtttttg gtgtatcaag
    32521 caaatttgag attgcctgat tagatactgg aatctctaga cctgcttttt taaccttatc
    32581 tcgaagaata ctagctccca tagcagagct tcccatacca gcatcgcagg cgaaaatgat
    32641 tttttccact gagtctgtcg aaacaactgc atcaacagaa gttgatacta actgaccttt
    32701 agactgagcc ttagctgctt gggtagccgc ctgagcagct tcgagcgaat cctcagttga
    32761 cttgtctgca tgaagaataa gggctgctac aaggaaagaa acaactgctg ccactaaaac
    32821 acctaaaaga acatttagat ggggccaaac accttttggc gccgtagcta taatcgcaat
    32881 aatagaacct ggtgaagctg gagatttaag accagcgtct aagagttgaa aagtaaaagt
    32941 tccagagata cctcctgcca tagcagctaa aaatagagta ggcttcatca taacataagg
    33001 aaagtaaatt tcatgaatcc ctccgaagaa atgaataacc attgcccccc aagaagaaga
    33061 tttagcagaa cctttaccga atacagcata agctaataga attcccagac ctggtccagg
    33121 attagcttcc aataggaaga gaattgactt accagcttga gctacctgtt ctactcccag
    33181 aggagtaaaa atgccatgat tgagggcatt attgaggaaa aggactttag ccggttcgat
    33241 gataatatta gccataggaa ggaggcgagc attgacaata gcctcaacac cattcccaac
    33301 agctccagta agagtcgata cgactggacc gattgcgtag aaagccaaaa gcaataatgc
    33361 aaaaccaacg agaccagctg agaagttatt aactaacatt tcaaatccgg gacgaatttt
    33421 ttcctggaac ttctcatcaa atttcttgat agtccatcct cccagtgggc ccattaccat
    33481 agctccgata aacataggaa cactagaacc tgtgattgca ccaacagtag caatagctcc
    33541 tacaacggca ccacgttggc catggatcat atatccacct gtgtaaccaa tcaggattgg
    33601 caataaatac gttaacatag gaccaacaac agtagctaac tgttcatttg gcagatagcc
    33661 atcagcgata aagagggcag tcaatactcc ccaagcaata aatgctccaa tattgggcat
    33721 aaccatattt gaaagcgatg tccctagttt ttgaaccctg actttcaatg atactttttc
    33781 ttccattaga tcatcctcct tttcttaaaa ctagtatata caaagttaaa aacctttaaa
    33841 actaaagttt tgtcacagat tttgtgtcaa aacattactt ttgacgaggc tgggcaaaaa
    33901 ggcttaaaaa ttataaaacg catcatatca ggtgtacatg aactacaccc caaaagttag
    33961 acagaaaaaa tctaactttt ggggtgtttt tattatgaaa ctaacttatg atgataaagt
    34021 tcagatctat gaacttagaa aacaaggata tagcttagag aagctttcaa ataaatttgg
    34081 gataagcaat tctaatatta ggta
//
LOCUS       DBDESG010000017        33887 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.017, whole genome shotgun sequence.
ACCESSION   DBDESG010000017 DBDESG010000000
VERSION     DBDESG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.017"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(186..1748)
                     /gene="guaA"
                     /locus_tag="ACKB38_000805"
     CDS             complement(186..1748)
                     /gene="guaA"
                     /locus_tag="ACKB38_000805"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGL7979607.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1890..2588
                     /locus_tag="ACKB38_000806"
     CDS             1890..2588
                     /locus_tag="ACKB38_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGL7979608.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2631..3527)
                     /locus_tag="ACKB38_000807"
     CDS             complement(2631..3527)
                     /locus_tag="ACKB38_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGL7979609.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVAFPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3536..4471)
                     /locus_tag="ACKB38_000808"
     CDS             complement(3536..4471)
                     /locus_tag="ACKB38_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGL7979610.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4468..5193)
                     /locus_tag="ACKB38_000809"
     CDS             complement(4468..5193)
                     /locus_tag="ACKB38_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGL7979611.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5277..5912)
                     /locus_tag="ACKB38_000810"
     CDS             complement(5277..5912)
                     /locus_tag="ACKB38_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGL7979612.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5914..6741)
                     /locus_tag="ACKB38_000811"
     CDS             complement(5914..6741)
                     /locus_tag="ACKB38_000811"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGL7979613.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6763..7098)
                     /locus_tag="ACKB38_000812"
     CDS             complement(6763..7098)
                     /locus_tag="ACKB38_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGL7979614.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7099..7689)
                     /locus_tag="ACKB38_000813"
     CDS             complement(7099..7689)
                     /locus_tag="ACKB38_000813"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGL7979615.1"
                     /translation="MGDFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNH
                     ILDSQIEGVISTADIIEKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYGFNG
                     IEQYISQEDYNRYLSDLNEDKMKVEIERAEKEADITIIMLQMGVEYRLEPTEEQKALY
                     HKMIDLGADIIFGGHPHVVEPSETVEKDGDKKLIIY"
     gene            complement(7705..7827)
                     /locus_tag="ACKB38_000814"
     CDS             complement(7705..7827)
                     /locus_tag="ACKB38_000814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979616.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQGD"
     gene            8350..8961
                     /gene="def"
                     /locus_tag="ACKB38_000815"
     CDS             8350..8961
                     /gene="def"
                     /locus_tag="ACKB38_000815"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGL7979617.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9006..9734
                     /gene="rlmB"
                     /locus_tag="ACKB38_000816"
     CDS             9006..9734
                     /gene="rlmB"
                     /locus_tag="ACKB38_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGL7979618.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="ACKB38_000817"
     CDS             complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="ACKB38_000817"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGL7979619.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10750..10974)
                     /locus_tag="ACKB38_000818"
     CDS             complement(10750..10974)
                     /locus_tag="ACKB38_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGL7979620.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11052..11795)
                     /locus_tag="ACKB38_000819"
     CDS             complement(11052..11795)
                     /locus_tag="ACKB38_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7979621.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11795..12595)
                     /locus_tag="ACKB38_000820"
     CDS             complement(11795..12595)
                     /locus_tag="ACKB38_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7979622.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12753..13109)
                     /locus_tag="ACKB38_000821"
     CDS             complement(12753..13109)
                     /locus_tag="ACKB38_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGL7979623.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13103..13633)
                     /locus_tag="ACKB38_000822"
     CDS             complement(13103..13633)
                     /locus_tag="ACKB38_000822"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGL7979624.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(13633..14127)
                     /locus_tag="ACKB38_000823"
     CDS             complement(13633..14127)
                     /locus_tag="ACKB38_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGL7979625.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKMPVDGERLAYQ
                     KLKK"
     gene            14262..14708
                     /locus_tag="ACKB38_000824"
     CDS             14262..14708
                     /locus_tag="ACKB38_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGL7979626.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14705..15352
                     /locus_tag="ACKB38_000825"
     CDS             14705..15352
                     /locus_tag="ACKB38_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGL7979627.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="ACKB38_000826"
     CDS             complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="ACKB38_000826"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGL7979628.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="ACKB38_000827"
     CDS             complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="ACKB38_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGL7979629.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16982..18361)
                     /gene="nox"
                     /locus_tag="ACKB38_000828"
     CDS             complement(16982..18361)
                     /gene="nox"
                     /locus_tag="ACKB38_000828"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGL7979630.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18655..19578
                     /locus_tag="ACKB38_000829"
     CDS             18655..19578
                     /locus_tag="ACKB38_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGL7979631.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19638..20243
                     /locus_tag="ACKB38_000830"
     CDS             19638..20243
                     /locus_tag="ACKB38_000830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGL7979632.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20262..21506
                     /locus_tag="ACKB38_000831"
     CDS             20262..21506
                     /locus_tag="ACKB38_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGL7979633.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21729..21986)
                     /locus_tag="ACKB38_000832"
     CDS             complement(21729..21986)
                     /locus_tag="ACKB38_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGL7979634.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22028..24064)
                     /gene="glyS"
                     /locus_tag="ACKB38_000833"
     CDS             complement(22028..24064)
                     /gene="glyS"
                     /locus_tag="ACKB38_000833"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGL7979635.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="ACKB38_000834"
     CDS             complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="ACKB38_000834"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGL7979636.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25564..26324)
                     /locus_tag="ACKB38_000835"
                     /pseudo
     CDS             complement(25564..26324)
                     /locus_tag="ACKB38_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(26597..27438)
                     /locus_tag="ACKB38_000836"
                     /pseudo
     CDS             complement(26597..27438)
                     /locus_tag="ACKB38_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(27552..28943)
                     /gene="pgdA"
                     /locus_tag="ACKB38_000837"
     CDS             complement(27552..28943)
                     /gene="pgdA"
                     /locus_tag="ACKB38_000837"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGL7979637.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQYQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29181..29549)
                     /locus_tag="ACKB38_000838"
                     /pseudo
     CDS             complement(29181..29549)
                     /locus_tag="ACKB38_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29770..30747
                     /locus_tag="ACKB38_000839"
     CDS             29770..30747
                     /locus_tag="ACKB38_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGL7979638.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30744..31826
                     /locus_tag="ACKB38_000840"
     CDS             30744..31826
                     /locus_tag="ACKB38_000840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGL7979639.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31890..32443)
                     /locus_tag="ACKB38_000841"
                     /pseudo
     CDS             complement(31890..32443)
                     /locus_tag="ACKB38_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(32477..33748)
                     /locus_tag="ACKB38_000842"
                     /pseudo
     CDS             complement(32477..33748)
                     /locus_tag="ACKB38_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10000 a         7550 c         6073 g        10264 t
ORIGIN      
        1 attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat tttttgtcca
       61 aatgtaaacc acttggaact attattcaat ttattctttt tagcaaatgt tcccgtttta
      121 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
      181 tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
      241 ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga
      301 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
      361 tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg
      421 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
      481 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
      541 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
      601 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
      661 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
      721 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
      781 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt
      841 tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
      901 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
      961 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     1021 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     1081 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     1141 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     1201 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     1261 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     1321 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     1381 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     1441 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     1501 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     1561 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     1621 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     1681 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     1741 ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa
     1801 gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat
     1861 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatataaaa atccatgatc
     1921 agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt cccagtgaaa
     1981 gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat
     2041 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     2101 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     2161 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     2221 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     2281 accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta
     2341 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg
     2401 gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     2461 aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta
     2521 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa
     2581 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     2641 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     2701 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt
     2761 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     2821 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     2881 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaaaggc gaccaaacct
     2941 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     3001 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     3061 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     3121 gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     3181 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     3241 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     3301 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     3361 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc
     3421 agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca
     3481 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     3541 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     3601 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     3661 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     3721 aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat
     3781 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac
     3841 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     3901 ctctaactgt tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag
     3961 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     4021 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     4081 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     4141 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac
     4201 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     4261 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     4321 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     4381 gattgtcctt aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt
     4441 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     4501 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     4561 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     4621 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     4681 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     4741 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     4801 tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca aatcatcttc
     4861 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     4921 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     4981 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     5041 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     5101 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     5161 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     5221 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     5281 taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     5341 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     5401 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc
     5461 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct ctcattgaca
     5521 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     5581 atagcttcga gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc
     5641 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     5701 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca
     5761 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     5821 tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc ttgttccttt
     5881 aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     5941 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     6001 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     6061 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     6121 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     6181 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     6241 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     6301 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     6361 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     6421 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct tgttgacaat
     6481 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     6541 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     6601 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     6661 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     6721 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     6781 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     6841 ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata
     6901 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     6961 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     7021 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     7081 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt
     7141 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc ccaaatcgat
     7201 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     7261 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat
     7321 cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc
     7381 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     7441 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     7501 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     7561 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     7621 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     7681 atcaccaata gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc
     7741 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
     7801 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
     7861 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
     7921 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
     7981 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
     8041 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcgttc
     8101 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
     8161 caattgaatt gaacttccta tctattttct ataaatccta aatgccataa tgctttcaat
     8221 tcttgtcatt ttgtgatatc atgtagaggt aatgaaagaa aatgaactaa tccacagtgg
     8281 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa
     8341 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
     8401 gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt cactttcccc
     8461 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
     8521 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
     8581 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
     8641 actccacagg aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac
     8701 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
     8761 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
     8821 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
     8881 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
     8941 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
     9001 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gccctccttg
     9061 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
     9121 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct
     9181 ctgagattac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
     9241 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga
     9301 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
     9361 ccaatgtttc aggtgtcatc attcccaagc accgtactgt cggagtaact cctgtcgttg
     9421 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgacc aacctcagtc
     9481 aaaccttaga taaacttaag gatgaaggtt tctggacctt tggaacggat atgaacggta
     9541 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
     9601 aaggtatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
     9661 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
     9721 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
     9781 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca accgctgttg
     9841 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
     9901 ttttcatgtg gttatctgtc acaatccatc ctgcctgatc ttggatattc aattctttaa
     9961 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg
    10021 tcacttgacc tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga
    10081 tttcccttac tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt
    10141 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
    10201 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
    10261 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
    10321 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
    10381 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
    10441 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
    10501 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
    10561 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
    10621 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
    10681 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
    10741 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
    10801 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccctggctgt
    10861 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
    10921 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
    10981 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
    11041 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
    11101 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
    11161 aagaagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
    11221 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
    11281 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
    11341 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
    11401 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
    11461 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
    11521 aacaaattaa actgttggaa aaccatagac aacttacgac gtagggcaag gatttcttct
    11581 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
    11641 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
    11701 aaggctacaa cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct
    11761 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
    11821 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt
    11881 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
    11941 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
    12001 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
    12061 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
    12121 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
    12181 tagcataagc tgcctcatta aaggcaaagg cgacaatcgc aaaagctgca gctggaatcg
    12241 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
    12301 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
    12361 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
    12421 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
    12481 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa
    12541 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
    12601 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
    12661 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
    12721 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ctcataagat
    12781 tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac
    12841 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
    12901 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
    12961 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
    13021 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
    13081 catttggggt attcgataaa ttctaacatg tgcttctcct tctattgctt ctatctttaa
    13141 aatctactcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcataacctg
    13201 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    13261 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    13321 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    13381 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    13441 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    13501 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    13561 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagg atttttacat
    13621 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcat
    13681 cttaccgact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    13741 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    13801 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    13861 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    13921 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    13981 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    14041 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    14101 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    14161 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    14221 ttataatcta gttatcaaaa ctatataaaa aggagttaaa aatgaaaact accttttcct
    14281 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    14341 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    14401 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    14461 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    14521 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    14581 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    14641 aaaccgttaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    14701 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    14761 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    14821 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    14881 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    14941 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    15001 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    15061 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    15121 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    15181 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    15241 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    15301 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    15361 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    15421 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    15481 aatttgattg ttcactgttg ctaaaatttc tacaccctca ccaacactac tgataatcgg
    15541 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    15601 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    15661 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    15721 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    15781 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    15841 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    15901 cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt tcatctattt
    15961 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    16021 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    16081 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    16141 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    16201 agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca attctacagg
    16261 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    16321 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    16381 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    16441 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    16501 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    16561 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    16621 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    16681 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    16741 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    16801 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    16861 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    16921 tggggacaaa ttagctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    16981 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggtttg ttgaagtgtg
    17041 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    17101 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    17161 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    17221 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    17281 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    17341 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg taggcaccaa
    17401 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    17461 tgtcataaac agtcgcacag tcaccaacag cgtaaacgtc tgggatagat gtttcttgtt
    17521 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccacct gcaagggctg
    17581 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    17641 taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga cctagagcca
    17701 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    17761 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    17821 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgaggt
    17881 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    17941 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    18001 cgattggtgg caagattggt gtagagcctg tagcgaaaat caatttttcg tatgattctt
    18061 tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg attgaaagaa
    18121 caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta tcagaataga
    18181 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    18241 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    18301 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    18361 taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta ccgttagcgt
    18421 ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc catttatcct
    18481 gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa ttttgtttgt
    18541 gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg gcttcaattt
    18601 agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg tagcttgact
    18661 cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt agtggatgag
    18721 caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga ataccgcttc
    18781 aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg aatagccccc
    18841 gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca tagcctagcg
    18901 ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg tatcggtttt
    18961 tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct aatcaatcct
    19021 catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa aggaatgaag
    19081 atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca atttcttagg
    19141 aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac cattggaaaa
    19201 aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc caatcctagg
    19261 ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc aggcatttat
    19321 gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag tcaaacagga
    19381 tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc agtcgatggc
    19441 gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg gcaagtcgaa
    19501 agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag ctgttcttca
    19561 ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt attatgtaaa
    19621 aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt
    19681 ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa gctgaaattg
    19741 aacttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag caagtacctg
    19801 ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt atcggtactc
    19861 ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg
    19921 gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac ggtacgcttg
    19981 gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa
    20041 atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac ccaagtggcg
    20101 acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac ttccgtatct
    20161 ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa gatgctgaag
    20221 actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt gttggacttg
    20281 ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc cgtcgctact
    20341 tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg tttaaccaag
    20401 atgaaaaatg ggatgaaagt ttccaattac gttatttata taacaaaatt actcgtgcag
    20461 atggtgttat cattgctact cctgagcata accacactat ctcagcttca ctcaaatctg
    20521 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    20581 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    20641 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    20701 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    20761 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    20821 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    20881 accctgacga tccagaatgg gtagaaaaag ttgcagcatt cactggtgct gtttctggtg
    20941 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    21001 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    21061 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    21121 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    21181 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    21241 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    21301 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    21361 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    21421 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    21481 acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt
    21541 tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt tcatctatct
    21601 ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    21661 cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt gattttcgaa
    21721 gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt
    21781 acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct
    21841 tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct
    21901 tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg
    21961 atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta
    22021 tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta
    22081 gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag
    22141 tattttcaaa gaaagcatca atgactgggc taagcgcaaa aagttgtttc aattgctgac
    22201 ttgcaggtcc tgataaaatg agtgtttcta ctgcttctgc caaagctttt tcttggtcat
    22261 tctcaaatag tgctgaatca accgtagcaa ccccttctgc cttctcggcc aggttaaagg
    22321 cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag
    22381 cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttcct
    22441 tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa
    22501 cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca
    22561 gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg
    22621 cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac
    22681 tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa
    22741 gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag
    22801 tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac
    22861 caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg
    22921 tttcatccac tgacaaacta gctttttctg ccaaaagtac agtgatttga cccgtacgaa
    22981 tcatgtgttc acgaagagaa ccaatcttct catggaaggt gacattgttt aatttttcaa
    23041 caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc
    23101 gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt
    23161 tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac
    23221 gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat
    23281 caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat
    23341 ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa
    23401 tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat
    23461 agctcaatgc tgactgaatc ttggtttctt gtcccaaaaa acgatggcca cgactcacac
    23521 gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta
    23581 aagtgtgaac agggcggatg tattcaaagc tatttcccgc ccagtgcatg ctgacaggga
    23641 aagtcagtga cttcaagaca tccacaatgc ctggaacaat ggcttcaact gcttgaccaa
    23701 tttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat tcgatatctt
    23761 caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac
    23821 tatctaaggc aattttcttt gctggaccct tgaaatcttc tgttaaatca gactgtttgt
    23881 ctgcaagacc agttacacga acagccaaac gacgtggtgt tgagaaagtt tgaatggctt
    23941 caaaagacag gcgttttccc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac
    24001 ttggcgtaac aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg
    24061 tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg tgattggcac
    24121 gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct ccaattttgc
    24181 ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc tcagggctca
    24241 ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc tgctaggagt
    24301 ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc tgcgacaaag
    24361 gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt
    24421 acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag
    24481 tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta
    24541 tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca
    24601 tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag
    24661 acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca
    24721 gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc
    24781 attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag
    24841 ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagac
    24901 tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg
    24961 tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc
    25021 tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc
    25081 attgtccccg cacctttttc attatcataa gcctgcataa gcatacaatc ttggtcattc
    25141 caaaattgtt gcaaagtcaa aataatttct tgaaatgtta atttcttaga cattgaatac
    25201 tccttaataa aaaataattt cttgcgacac gagtctgcct cgtcgattat acgaggcaag
    25261 acgagttagt actgcgtcta gctcaggcac tctagtgctg agcgtagggc agtccgactg
    25321 taaggaggtt tctgccactg acgcagtgaa atgttaattt cttagacatt gaatactcct
    25381 taataaaaaa taattacttg cgacacgagt ctgcctcgtc gatcatacga ggcaagacga
    25441 gttagtactg cgtctagctc aggcactcta gtgctgagcg tggagcagcc cgactgtaag
    25501 gaggtctctg ccactgatgc agtgaaatgt taatttctta gacattgttt actcctttag
    25561 tttttatatt tctttttcaa ttaatttctg ctgaacaaac caatctaaca agggaaaagt
    25621 tttttctgta tttgcccata aatgataaga atctaagacc gcttgtacac tacctaaatt
    25681 ttctaccaac ttatctactg tcaaaaagct gcgccagtat tcataaaaaa tacttgcata
    25741 gttcttatca taagtcgaag aaccaaaatc attcaatgaa tgccaactat acttcttctg
    25801 aaaaagttct acgagagatt gattacaaat tttttccgct tgaaattcct cttctgtcaa
    25861 gaaatacttg cgactaatat attcaaccat cccctcttcg aaccagatat aagagtcata
    25921 accatcaaaa tcatctaaaa aatgtccgac caatgagcta actcatgtcc tacaatctgt
    25981 aagaggaaat tttcagataa agaatgatag tgactttcta ttgcttgagt ttgctcagaa
    26041 cactcgtagt ccatcaactg atacaaatac aattctttcc aaacagctaa atcaggcgtc
    26101 ataaccactc gtctattatt tgtataggtt ggaacagcac tttccctaat aatctgtgta
    26161 gcagcattaa aatctgacca aataacagct tgcggtaaat cacagactgc aagttcattc
    26221 tttaaaaaag attgataatc tttcaacttt tcagtatttc ttactacaaa atcacgaaat
    26281 gtagctagtt gactatcgtc ttttacaaga taaaggttgt ccacggctct cctttctttc
    26341 acacaataga ctgaataagc aaaagaacca catctgacaa tcctaggttg atttaaccta
    26401 ggggcgtcag aaacgcggtt ccaccctaat ttattacttc attgtttttg aaaatactac
    26461 agaaagcgcc agccctttat tttgccctac ttggctccca ctatcccaag ctcgcttgaa
    26521 gaacgccata agactttctt tcctgctgac tattatatca aaaattagaa taatgtacaa
    26581 ttctttttat gattttctag aaatccacat catcaactcg tggagcaccc gattgaacag
    26641 caatcgtttt taaggtttct ctctcctcat gactcagttc aattccaaag caatcaagat
    26701 tagcttgaat acgagaggtt gtgacagatt ttggaagtgg taaaaatcct tctgccaagc
    26761 tccaggccaa ggctatctga gcaaccgatt ttccgtgatt tgctgctatt tcttggactt
    26821 gcttgctatc aaacagttct ccttgtccaa aaggccccca agcttccaat aaaattccct
    26881 tttcacgaca gtaagctacg acttgatctt gatacacacc tggcgccaag cgaacttgat
    26941 tgaccgcagg aacgatagtt gcagtttcaa gcaaggcatc caaatgatgg ggaagaaaat
    27001 tgctaacgcc gatagcacgg attttccctt cttgatagag gtcttccatc gctctccaaa
    27061 cttccgcatt gcgagttttc catgcgtcat tttctctgag cggttttggg ttcggccaat
    27121 gaatcaaata caaatccaaa taatccaagc ccagtttttc tatagatttt tccaaagctt
    27181 gacgagtttg ctcataggtt tgctgactat tccaaagctt ggtagttacg aacatttctt
    27241 cacgtggaac tccgctatct ttgattgctt gaccaacact ttcttcattc tgataaatcg
    27301 ccgccgtatc aatatgacga taaccagcct tcaaggcttc taacactgca cgataggctt
    27361 cttctccatc cttagcctta aaagtccaaa tcccaatact ggaatttcta ctccattatt
    27421 taattgatag gtattcatat gcttctcctt ctattttatt catcaattat agcataaaac
    27481 attttttaag tagtactata ttctgtaaaa aataagtctg tcaaatatct aacagaccta
    27541 ttttttcttg cttattcatc acgactatag tacagctcat gagcttttag gcgagtattg
    27601 agcatctctg gtatggtcac aaaggtataa ccttgatttt tcaaatactc aatgaccctt
    27661 ggcaaggcat tgactgtcgg actgtgaata tcatgcatca aaacgataga gccattagct
    27721 acttgatact gaatttctgt caaaatagat gcttcatttt tactcttcca gtccagacta
    27781 tccacatccc acatgataaa gctcaaatcc aagctattgc gaatatcatc tgtaatagca
    27841 ccataaggtg gacgcatgag tttagaacta gaacccagca ctttagttag cacatcctca
    27901 gtatcagtaa tctgcttttt agcttcatca agagagagtt gcgagagaat cggatggctc
    27961 cagctatggt ttccaacaac atgaccttca gattttatcc tcttcaccaa gtcctcattc
    28021 ccagaaacat ttttcccaag cacaaagaaa gtcgctttaa tatcatattt agctagggtc
    28081 tctaatacct gcggggtcgt tgctggattt ggaccatcat caaaggttag agcgacaact
    28141 ttttgatgtt tcttatcaaa gtaagattgg tacaaggccg catctttttc gagtaagtac
    28201 gaagattgga taacatcaaa gaaagcagat actggcaagg ctatctcttc taaattttca
    28261 accacaggac ttggataaag gataatctga ctatccttgt aatcaaaatt ccatgcagac
    28321 aagtcttggt cagagaagtt ttttacaatc tgctcactct ggtcttgctc tattttttta
    28381 tcctctatga aggaggtcaa ctcttttatc agctgttcct tagccttact agcatctgaa
    28441 aacagttggt caagtgtaaa aggttgccca tcttctgtta agtgaacctt tcctagactg
    28501 gtcttttcag tctcttcaat cttaaaagca actaaatcat agatttgttt ggtcacatta
    28561 cgattaacga ctccctttaa acctgactct tcttgctctg tatagtagaa aacaagattg
    28621 tccttacttt ctagcttgtc cttaacatct tgatttatca gctccctaac agaggaaatc
    28681 actttctccc cttggagagg ataataggca atcacttcgg cttgcccctg acgaaaatgc
    28741 tccttctgat ttccctcact caattgatca tctttctctt ttttgagcga ttcaatcttt
    28801 tgctcaaaac ttttttgctg gtagatctta aaagcaataa atccgcctaa taggcaaata
    28861 gaaattgcta aaataccaat caaagccaat aatacatgtc tcgttttccc gtgtctgcca
    28921 cgtcctagtc tacttttatt catatttata tcataacaaa tttgagcaat aatttcaatg
    28981 atacaatttg gcaatccaca attcctctag caagccaaag catcttacat caactccaac
    29041 atccgtatct gtagagatta tgtagttttc tatctatgga atttctagta gattgaagct
    29101 agaatagtag acctatactt ctaaaacatt gttagaaatc gatttgactg tcctgatcga
    29161 tttgtcctgt tctcatttca ttttactata aaactatgaa aagctaggtc atctgagaag
    29221 taattactat cgacaattgc gatagcgtat actagtgcga tttgtttgta gctctggtgg
    29281 gtatcacctt cacgttggcg aattttttct aggttttgat taacctgact gcacagataa
    29341 ggccataggc gattgatgaa aaaattctga tgatgaacct gaatttcgat gataacttgg
    29401 attccattat ctaactccgc caagacgtct atactagtat agaaatctta cgctgagtag
    29461 ggcatggaag gcaagacgtg aatgttactt ccctccaaaa tcgtcacatt ttttgctggc
    29521 aagtccagca tatcgcggat aaattgacaa atcattagtt gggctgatac ctagatgtct
    29581 gagaatcctc cttttcctcc ttctattata gcacattttt aaatctaggt ttgctagatt
    29641 ctatgcttct atctatttat tcggaaaaga aagctaatag cttatttgaa attttctcct
    29701 ctctgttgga tagtcccgtc tatctatgtt ataatgaaag aaggatttaa aatcggaaaa
    29761 ggagtattta tgctaaaatt aggtgtcatc ggaactggcg ctatcagcca tcatttcata
    29821 gaagcagccc ataccagtgg agaataccaa ctggtcgcaa tctattctag aaaactagaa
    29881 actgcagcaa cctttgcttc tcgctatcag aatatccaac tattcgatca attagaagtc
    29941 ttcttcaaat cttcttttga tttagtctat atcgccagtc caaactcctt acattttgct
    30001 caggcaaaag ctgccttgtc tgctggtaaa catgttattc tcgaaaagcc agctgtctct
    30061 caaccacaag aatggtttga tttgattcaa acagctgaaa aaaataactg ttttatcttt
    30121 gaagcagctc gtaattacca cgagaaagcc tttactacta tcaaaaactt tttagcagat
    30181 aagcaagtgt taggagcaga tttcaactat gccaaatact cttccaagat gcctgacttg
    30241 ttggctggac aaacaccaaa tgtcttttca gaccgttttg ctggtggagc cctcatggat
    30301 ttggggattt atcccctcta tgctgctgtt cgtctttttg gaaaagccaa tgacgcgact
    30361 taccatgctc aacagcttga caatagcatt gacctaaatg gagatggtat cctcttctac
    30421 ccagactatc aagttcacat caaggctgga aaaaacatca cttccaatct tccttgcgag
    30481 atttatacaa cagatggaac cttgactctc aacacgattg agcatattcg ctcagctatt
    30541 tttaccgacc accaaggaaa tcaagtccag cttcctatcc aacaggctcc tcatacgatg
    30601 actgaggaag tcgctgcatt tgcacacatg atccagcaac cagatctgaa tctctaccaa
    30661 acttggctgt atgatgcagg ctctgttcat gagctactat ataccatgcg ccagactgct
    30721 ggcattagat ttgaggcaga aaaatgaaaa ccaaactacc gactgaatgg caagaactga
    30781 gtgaccaact cggtttccaa gaattcaccc ccattcaaac tcaactattt gagccccttc
    30841 ttgctggaga aaacctccta ggagtgagcc aaaccggaac tggtaagacc ctagcgtatc
    30901 tcctaccaag tcttctcaga ctacaaaaga aaaaagccca acaactcttg attctagcac
    30961 caaatacgga acttgctgga cagatttttg atgtatgtaa aacgtgggca gaagctatcg
    31021 gattgactgc tcaactcttc ctttcaggtt caagtcagaa acgacaaatt gaacgtctta
    31081 aaaaggggcc agaaattctg attggaactc ctggccgtat ctttgaactc attaaactta
    31141 aaaaaatcaa gatgatgaat gtggaaacca tcatcctgga tgaatttgac caattactag
    31201 atgattctca gattcacttt gtagaaaaaa tcactcacta cgcacctcgt gaccaccaac
    31261 tggtctacat gagtgccacg accaagtttg accaagaaaa gattgtgccg aatacacgca
    31321 ctattgatct ctctgatcaa aaattggaca atatccagca tttctacatg caggtagacc
    31381 aacgtcatcg agtggatatg ctacgaaaac tggctcatgt agaggatttc cgtggtctag
    31441 tcttcttcaa tagcctgtca gaccttggga atgccgagga aaaactacag tatcgtgata
    31501 tattggctgt ttccctcgct agtgatgtta atgttaaatt tagaaaaatc atcttagaaa
    31561 agtttaaaga taaccaacta accctgcttc ttgcaactga ccttttggct cgtggaattg
    31621 atatcgatag cctagaatgt gtcgtaaact ttgatattcc tagagatagc gaaacctaca
    31681 cacaccgtgc tggccgtact ggtcgtatgg gcaaggaagg ttatgttatc actctcgtca
    31741 ctcatccaga agaaatcaaa aaacttaaaa agtttgcaag tatccgagaa attgtcctaa
    31801 aaaaccaaga actctatatc aaataaagcc ccattgctct atacacatcc agtgtatgga
    31861 gcttttttat aagcaattca attttatact catctgtttt caaaaagcat tctagtccat
    31921 ctccgattaa cgatggactt tatcacctcc ttctccaatc cttgtatgac atcttggagt
    31981 tgattcatga catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc
    32041 tctttccaca cttgttcaat gggttcatct ctggtgtgta tggaggaata aaggtaaaac
    32101 caatattagt cggaatcttt aaggtacttg atttatgcca tatagcattg tccataacga
    32161 gtaaaagata atcatctgga taagcttgtg aaagctcttc taaaaaggcg ttcatccact
    32221 cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatgggca tcaacagctc
    32281 cataacaata gcgaaattct cgtatatagt gactatggac atgtggacct actcctattg
    32341 gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca
    32401 agcgaacctt atgaaaaggt ctactggttt taaagcgctt tcttgtgtac tgcccccaaa
    32461 cagttagaca aataatttat ccaaaggatt tagttctgta ctgcacagga ctaagtcctt
    32521 ttagttttat cttaattttc ttattgttgt aataatcaat atagtctata atggcttgtt
    32581 ccaattggtt aagtgattta aatgttttct catagccata aaacatttcg gattttaaaa
    32641 tgccaaagaa agattccatc ctaccgttgt cttggctgtt gcccttacgt gacatggatg
    32701 cttgaattcc cttactctct aggaaccgat gataagaatc gtgttgatat tgccagcctt
    32761 ggtcactatg gagaatcgta ttctcgtagt gcttctcttt gaatgcctgt tccaacattg
    32821 tttgtacttg ttctaaatta ggcgaacaag aaagattaaa agcaataatt tcactgttaa
    32881 agccatctaa aactggtgat aagtaaagct tttgagtact tgctggaatg gcaaattctg
    32941 tcacatctgt gtagcacttt tccattgttt tagagccttc aaattgtcct tgaatgagat
    33001 tctctgcctt cttgccaacg tctcctttat gagaagaata ttttcgtttc tgtcgcattt
    33061 tagcttgtaa attgagtact tttatcaagc cttgaactct tttatgattt accagataac
    33121 cacgatttct tagttctaaa taaatccgac gataaccata atttcccttg tgttcgataa
    33181 aaatggattg aatttcagct ttaagctctt ggtccttatc tggtttatct agctgtttca
    33241 agtgatagta gtaggtcaaa cgagctagtt taatggcttt tagaagaata tctaacgaaa
    33301 actcagtcat taattcttga ataatttctg tctttcttct ttctcttttt cctccttcaa
    33361 tcggagttct cttaactttt ttaggatggc attctccgct ctcaggtact ctccctcttg
    33421 ttttctcaac aatagtatac ccgtttttcc tgtattgtgc tagccagtta agaagtatcg
    33481 tacgacttgg gagacagtat tcaagagaaa ctctatcttt agtccagcct tcatgtcaga
    33541 ctttatgaat catttcttgt tttaaatcag gagaatagta acgatttttt ccttttttga
    33601 cgaactctat tccgtaacga tcaatcaatt taatcatgta cctaagatta gaattgttta
    33661 tcccaaattt atttgaaagc ttctctaagc tatatccttg ttttctaagt tcatagatct
    33721 taactttatc atcataagtt aatttcataa taaaaacacc ccaaaagtta gattttttct
    33781 gtctaacttt tggggtgcag ttcacttgtc ttcttgaatt gagactttat ttttagacgc
    33841 gacaatggtt tgagcatctg ctttcttagg atgttctgga cgtggcg
//
LOCUS       DBDESG010000018        33808 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.018, whole genome shotgun sequence.
ACCESSION   DBDESG010000018 DBDESG010000000
VERSION     DBDESG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.018"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            129..1622
                     /locus_tag="ACKB38_000843"
     CDS             129..1622
                     /locus_tag="ACKB38_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGL7979640.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2162
                     /locus_tag="ACKB38_000844"
                     /pseudo
     CDS             1635..2162
                     /locus_tag="ACKB38_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            2174..2689
                     /locus_tag="ACKB38_000845"
                     /pseudo
     CDS             2174..2689
                     /locus_tag="ACKB38_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            2821..3057
                     /locus_tag="ACKB38_000846"
     CDS             2821..3057
                     /locus_tag="ACKB38_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGL7979641.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            3054..3467
                     /locus_tag="ACKB38_000847"
     CDS             3054..3467
                     /locus_tag="ACKB38_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGL7979642.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            3585..4550
                     /gene="xerA"
                     /locus_tag="ACKB38_000848"
     CDS             3585..4550
                     /gene="xerA"
                     /locus_tag="ACKB38_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGL7979643.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            4573..5685
                     /locus_tag="ACKB38_000849"
     CDS             4573..5685
                     /locus_tag="ACKB38_000849"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7979644.1"
                     /translation="MFEEYPDSVFLDTYIKELRAGKSLAGEENNKNKVLKTGAVSYDY
                     FNSSEVKNLPIDYIPLDEHKVEIGDVIISRMNTSELVGAAGYVWAINSDNIYLPDRLW
                     KVILNDRVNPVFLWKLITNEKTKLKIKRISSGTSGSMKNISKSQLLQIRVPFPPLALQ
                     NEFADFVALVDKSQFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFL
                     NTKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKH
                     LRINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPL
                     ALQNEFADFVVQVDKSQFACEIAIKVWRNSLKFSII"
     gene            5715..9065
                     /locus_tag="ACKB38_000850"
     CDS             5715..9065
                     /locus_tag="ACKB38_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGL7979645.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(9571..10041)
                     /locus_tag="ACKB38_000851"
     CDS             complement(9571..10041)
                     /locus_tag="ACKB38_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGL7979646.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(10058..12331)
                     /locus_tag="ACKB38_000852"
     CDS             complement(10058..12331)
                     /locus_tag="ACKB38_000852"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGL7979647.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            12678..15779
                     /locus_tag="ACKB38_000853"
     CDS             12678..15779
                     /locus_tag="ACKB38_000853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGL7979648.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            15861..16868
                     /gene="pfkA"
                     /locus_tag="ACKB38_000854"
     CDS             15861..16868
                     /gene="pfkA"
                     /locus_tag="ACKB38_000854"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGL7979649.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            16927..18432
                     /gene="pyk"
                     /locus_tag="ACKB38_000855"
     CDS             16927..18432
                     /gene="pyk"
                     /locus_tag="ACKB38_000855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGL7979650.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(18817..18996)
                     /locus_tag="ACKB38_000856"
     CDS             complement(18817..18996)
                     /locus_tag="ACKB38_000856"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGL7979651.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRME"
     gene            complement(19329..19436)
                     /locus_tag="ACKB38_000857"
                     /pseudo
     CDS             complement(19329..19436)
                     /locus_tag="ACKB38_000857"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
     gene            20236..21108
                     /locus_tag="ACKB38_000858"
     CDS             20236..21108
                     /locus_tag="ACKB38_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGL7979652.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTESTTDVKVHAEKMEAFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(21254..21598)
                     /locus_tag="ACKB38_000859"
                     /pseudo
     CDS             complement(21254..21598)
                     /locus_tag="ACKB38_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            21741..22052
                     /locus_tag="ACKB38_000860"
     CDS             21741..22052
                     /locus_tag="ACKB38_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979653.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            22056..22772
                     /locus_tag="ACKB38_000861"
     CDS             22056..22772
                     /locus_tag="ACKB38_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGL7979654.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(22901..23716)
                     /locus_tag="ACKB38_000862"
     CDS             complement(22901..23716)
                     /locus_tag="ACKB38_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGL7979655.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKITSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(23713..24618)
                     /locus_tag="ACKB38_000863"
     CDS             complement(23713..24618)
                     /locus_tag="ACKB38_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGL7979656.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(24619..24750)
                     /locus_tag="ACKB38_000864"
     CDS             complement(24619..24750)
                     /locus_tag="ACKB38_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGL7979657.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKVKAEKKN"
     gene            24960..26327
                     /locus_tag="ACKB38_000865"
     CDS             24960..26327
                     /locus_tag="ACKB38_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HGL7979658.1"
                     /translation="MLHQGDKNSLLESLSIVERQTLKDIEVIIVNQSTEEIASISQQM
                     NLSFNIKIIDENSFAELSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGH
                     FADLVDGVFYFYPFWGDNWLTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLR
                     KINQFDINNLIWRLYIHSKTATYINFPTYIYKPVIDAKPLKDSYKIILENYENRIKDC
                     AVLENYDIERAKKQYIQELANFSAWLKNDGEYLDSDIVYHKLIAAEKGIFPFLELDRL
                     DFTIISNNCVGGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFEEELI
                     SPSWTHEVYPLGHLGDIDIHFLHYSSIEEARTKWEKRKKRINWNNLYFKFDNKDSASE
                     EILNKMDNLPYSNKLILVNRPYKNLKSQCVIPGQEHLPELAIMSDPLNTFDIMAWLKK
                     GGNAL"
     gene            26429..28558
                     /locus_tag="ACKB38_000866"
     CDS             26429..28558
                     /locus_tag="ACKB38_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGL7979659.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            28545..28994
                     /locus_tag="ACKB38_000867"
     CDS             28545..28994
                     /locus_tag="ACKB38_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGL7979660.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            29052..29324
                     /locus_tag="ACKB38_000868"
     CDS             29052..29324
                     /locus_tag="ACKB38_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGL7979661.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(29372..29536)
                     /locus_tag="ACKB38_000869"
     CDS             complement(29372..29536)
                     /locus_tag="ACKB38_000869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979662.1"
                     /translation="MDEAGEILLILFKNQSANYINFTLPTKLQPAAGFLVCTLIFIEY
                     KSKKALNQAV"
     gene            complement(29678..29869)
                     /locus_tag="ACKB38_000870"
     CDS             complement(29678..29869)
                     /locus_tag="ACKB38_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979663.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            30297..31055
                     /locus_tag="ACKB38_000871"
     CDS             30297..31055
                     /locus_tag="ACKB38_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979664.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            31057..33045
                     /locus_tag="ACKB38_000872"
     CDS             31057..33045
                     /locus_tag="ACKB38_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979665.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNVSEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(33087..33761)
                     /locus_tag="ACKB38_000873"
     CDS             complement(33087..33761)
                     /locus_tag="ACKB38_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGL7979666.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGVVIGVASATTNIWIIF
                     LSGFTAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GEFKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSDYRIPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLL
                     GQLFSI"
BASE COUNT        10785 a         5736 c         7023 g        10264 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaagtcata ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attggaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagatcatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaatttcag ttagatgaac taaacttgct cgtcaaatcc cgatattccg
     2161 aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg gctactttta tcaatggcta
     2221 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa tacagaatct
     2281 gactaaaact tcaaaaggga taaattatta ttcgggtacg attgataaaa aatacattgt
     2341 tgaagctggt gatattctta tttcgtggtc gggtacatta ggggtttttc agtggtgtgg
     2401 aagatccgca gtgttaaatc agcacatttt taaagtggtt tttgataaga tagatataga
     2461 taaatcgtat tttaaatatg ttgttgaaaa aggtttgcaa gatgctgtaa aacatactca
     2521 tggttccaca atgaaacatt taactaaaaa atattttgat aatattatgg tttcatatac
     2581 aaatttgaga gagcaacaaa gaattgcaag tgagctggat ttgttaagca agctgatttt
     2641 aaggagacaa gaacaacttg aagagcttaa tctacttgta aaatcccgat tggcaatcca
     2701 aaaatctctg gaagaacttg aaactttgaa gaaatctctg atgcaggagt attttggctg
     2761 atattctgcc attgtaatta cggtaatgat ttgttataat acttcaaagg aggaaatcag
     2821 atggtagtaa aaacaagaaa acaaggaaat tcaatcacca ttacgattcc aagtgaattt
     2881 aatattccaa gtggtgttaa atacgaagcg aaattgttac caagtggtga gattatcttt
     2941 actcctgaag aattggggca gcaggtttct tatgtatctg atgatgcctt tgacttaaat
     3001 ttagataaaa tatttgacga atacgacgat gttttcaaag ctttggtgga aaaatgacaa
     3061 tctatttgac agaaaagcaa attgaaaaaa taaatgcttt agcaattcaa cggtattctc
     3121 caaatgagaa aattcaaaca gttagtcctt ctgccttaaa tatgattgtg aacttaccag
     3181 aacaatttgt ctttgggaag cctctttatc caacaatttt tgataaagca acgatactat
     3241 ttgtccaatt gataaagaag catgtttttg ctaatgctaa taaaagaact gctttcttcg
     3301 ttttggtcaa atttttacaa ttaaacggct atcgtttttc tgtaacggta gaagaagcag
     3361 taaaaatgtg tgtaaccatc gcagtagaag ctttaactga tgaaaaaatg acaagctact
     3421 ccaaatggat ttctgaacat tctgttagag aaaaggtcaa aaagtaacct agtatgctgg
     3481 atttgaatga gcacaagaaa ataaatgaac agacaatatt agaattctgt aatgcagaaa
     3541 ctgatattgt ctctttttat tgatgaataa gaaagtgaga aattatggaa tcaaaagtta
     3601 caattatcat gcaagaaatg ttacctcttt taaataatga acaattacta gcgttgagag
     3661 agagtttaga acatcatcta gtagacggaa aaaagcagca gaagtattcg aataataacc
     3721 tgttgcaact atttattacc gccaagcagg tagagggctg tagctcaaaa acaattcgtt
     3781 attatcagag gacgattgaa aacttgttta atgctattaa agagtctgtg acacaactca
     3841 caacagatga tttaaggagt tatttagcaa attaccagtc tgaaaaggat tgtagtaagg
     3901 caaatttaga caatattagg cgtatattgt cttctttttt tgcttggctt gagcaagagg
     3961 aatatatcat taaaaatccc attcgacgga taaagaaaat taagactgag caaaatgtga
     4021 aggaaactta tactgatgaa catttggaaa ttatgcgtga taactgtgaa aatttgagag
     4081 atttggcaat aatagaccta ctagcatcga caggtatgcg tgtaggggag cttgtacagt
     4141 tgaatcgttc agatattgat tttgaaaaca gagagtgtgt tgtctttggt aaaggaaaga
     4201 aggagagacc agtatatttt gacgctcgta cgaaaattca tttaagaaat tatcttaacg
     4261 acagaaaaga tagtcaccct gctctttttg taacgctagt tggaaaagtc cagaggcttg
     4321 gaattgctgg tgtagagatt cgcttaagaa agttaggaga caaactcggc atacaaaagg
     4381 ttcacccaca taagttcaga agaactttag cgactaaggc aattgataaa ggtatgccta
     4441 tcgaacaagt ccaaaaactg ctaggtcata gcaagattga cacaaccttg gcctatgcca
     4501 tggtcaatca aaataatgtc aagcattcac accaaaaatt catctcttaa aagcaaatcc
     4561 cgatttaacg agatgttcga agagtatcct gactcagtat tcttagatac atatataaaa
     4621 gaactaagag caggtaaaag tttagctggt gaggaaaata ataagaataa agtattaaaa
     4681 acaggagctg tatcgtacga ttattttaat tcatctgaag taaaaaattt accaattgac
     4741 tatattccct tagatgaaca taaagttgaa ataggggatg taattattag tcgtatgaac
     4801 acttctgaac tagttggggc tgctgggtat gtttgggcta tcaatagtga taatatatat
     4861 ttgccagata gattatggaa ggttattctg aatgatcgag taaatccagt tttcttatgg
     4921 aagcttatta caaatgagaa aacaaaatta aaaattaaga gaatttcctc tggaactagt
     4981 gggagtatga aaaatatttc gaaatcacaa ttgttacaaa ttagagttcc cttcccccct
     5041 ctagccctcc aaaatgagtt cgcagacttt gtagccctgg tcgacaaatc acaatttaac
     5101 gagatgtttg gggaaaataa aatatttgaa agcattgata acttatttga tattatagat
     5161 ggtgataggg gcaaaaatta tcctaaatca gatgagttgt ttagtgagga gtactgttta
     5221 tttttaaata caaagaatgt tactaaaaac ggattttcat tcgatacaaa gcaatttatc
     5281 actaaaacaa aggataaatt acttcgaaaa ggcaaacttg agcgttatga tatagtcttg
     5341 acaacaagag gtactgttgg aaatgtagcg tactacgatg aattaataaa atataaacat
     5401 ttacgtataa attcaggtat ggtaatatta cgtcccaaga caccaaatct aaatcagaaa
     5461 tttattatcc atgttttaag gaataataat tatagtcgag tgatatcagg aagtgctcag
     5521 cctcagttac caattacaaa attaaaaaaa atacttctcc ccctcccccc actagccctc
     5581 caaaatgagt tcgcagactt tgtagtccag gtcgacaaat cacaatttgc ttgtgagata
     5641 gctataaaag tgtggagaaa tagcttgaaa tttagtataa tatagctaaa ctatttgttt
     5701 aaagtgagaa aaaaatggga aattttagct ttcttttaaa aaatgacgaa tatgaatctt
     5761 tttcaaaacc ttgcattgaa gctgagaata tgattgctac atcaactgtg gctactgcct
     5821 ttatggcgcg tcgtgcttta gagcaggctg tccattggat atatagtcac gattcatatt
     5881 tagaagctcc ctatcgtgct actctatctt ctttagtatg ggatgatgat tttagggata
     5941 tcgtagattc tgaactccac aagcagatag ttctgttgat tcggtgggga aaccatgctg
     6001 ctcatggtgg tgaaattaag gaacgagaag cgattttagc tttgcatcat ttgtatcagt
     6061 ttgttaattt tatcgattat tgttacagca atgagtttgt ggagcgttat tttgatgaga
     6121 agtgcttacc actttcagca aacatcaaat accgagaaac tccacaatct atgataaagt
     6181 tacaagacag tttaccagaa ctgcctgatt ttcatgaaca gatggctgct cagtccgtag
     6241 aagttcaaga gacttatact gaaaaacgtg agactgcagc gcaacggcaa gatgtgcctt
     6301 tccatattga tcaattatct gaggcagaga caagaaagct ctttattgat atcgatctcc
     6361 gtttagcagg atggatattt gaagaaaact gtcgtgttga gatagccgtt gatggtctca
     6421 agcacggttc aggaattggt tactgtgact atgtacttta tggtaaaaat gggaaaattt
     6481 tagcgattgt ggaggctaaa aaagcctctg tcaatccaga agtaggggaa gtacaggtca
     6541 aagaatatgc tgaagctttg gagaaacata tcggctatca gccaatttgc tttattacaa
     6601 atgggttgaa gcactatata cttgatggtc cgaaccgccg ccagattgca ggcttttact
     6661 ctcaagaaga attgcaatta gtgatggata gacgtcatct tcaaaaaccg cttgaggata
     6721 tttctagtaa aattagggac gatatttccg ggcgtcacta ccaaaaacat gccattgcaa
     6781 gcgtttgtga agctttctct gatcatcgta gacaggcact tttggttatg gcaactgggg
     6841 cggggaaaac tcgtacagca gtttctctag ttgatatctt atcacgtcat aactgggtaa
     6901 aaaacgttct cttcttagcc gatagaactt ccttggttaa gcaagcatat gattcgttta
     6961 gaaaattact cccagatctt tccgtttgta acttcttaga agataaagaa ggagctcaat
     7021 caagtcgcat ggtcttttca acttatccga ccatgattgg agcgattagt ggtcaagaag
     7081 aagtaaatca acgccctttc actgttgggc attttgacct tatcataatt gacgaatctc
     7141 accgttctat ttatcagaaa tacaagtcca tttttgatta ttttgatgca agaattgtag
     7201 gcttaacagc tactccgcgt caagatttag ataaaaacac ctatggattc tttaatttgg
     7261 agaatggggt tccaacatat gcatatgatt tggaagaggc tgttaaagac ggatatttag
     7321 tagcctatca ttctatcgaa accaaactga aactacctac ggatggtcta cattatgatg
     7381 atttgtccga agaagaaaag gaacattttg atagcaaatt tgaagacaat agctgtgaaa
     7441 aagatattga tgggagtgta tttaattcct ttattttcaa taaaagtaca gtagaaattg
     7501 ttttaaatga actcatgaca agaggaattc agacagcctc gggtgatgaa attggtaaaa
     7561 ctattatttt tgctaaaaat catgatcatg cggaatatat cagaggtatt tttaacaacc
     7621 gctatcctga aaaagggagc gactatgctc aggtgattga ttatagtatt aagcattatc
     7681 agaccttgat tgatgatttt aaaattaagg agaagtatcc tcaaattgcg atttctgtcg
     7741 atatgttaga tacaggtatt gatgtaccag aggttgttaa tttagtcttc ttcaagaaag
     7801 tacgctctaa aactaagttt tggcagatga ttggtcgagg aacccgtcta tgtaaagatt
     7861 tatttggacc tgagcaggat aaggaaaact tcttggtatt tgattatggg gacaattttg
     7921 attattttcg tgcagatcca agagatggag agggtcgtca cattgtttcg ctgactcagc
     7981 gtttatttaa tatcaaagtg gacttgattc gagaacttca gggactccaa taccaagaag
     8041 atcagtttgc gagagcatac cgtcagcagc ttgtctcgga acttcaaggt cgtatagaga
     8101 gcttaaatga gttggacttc agggttcgta tggttttaga tacagtttat agctatagga
     8161 aattggaaag ttggcagaat ctaactgctg ttacaagtga aaccattcaa aaaaatctct
     8221 ctccgctttt atttgatgaa gataaagaag atgagatggc gaggagattt gatttgtggt
     8281 tgcttcatat tcagttgggg caactgacag ctaaatcttc cactgttcat atttcccaag
     8341 tgatgaagac ggctagagct ctttctgcta ttggcaatat cccgcaggtt tttgagcagg
     8401 ctgaaattat caggaaagta caggagcctg aattttggaa agaagttaac ttgtctgatt
     8461 tggaaaaaat tcgtcttgct attcgagatt tattacagtt tttggataaa acagaccgta
     8521 aaccctacta tgttaacttt gaagatcgta tactctccac tgttcacgag accacagcat
     8581 ttttgcaggt caacgatctt cggtcttaca atgaaaaagt tgagcattat ttgaaaactc
     8641 atctggatga ggagtccatt tctaagctat accataataa aaagttgaca tctgatgata
     8701 tgcttgcact tgaaaaattg ctttgggaaa aattaggtag taaagcagac taccaaagtc
     8761 attatgaaaa taaggcaatt ccgagattgg ttcgtgagat tattggctta gatagagagt
     8821 ctgccaatcg tattttttct aaatttttgt cggatgagaa tcttaatgcc aggcagattt
     8881 catttgtaaa attgattgta gactacattg tagaaaatgg ttttttagag acgaaagtgt
     8941 taacgcaaga gccgtttaaa tcttatggtt ctgttcaact actcttccaa caccaactac
     9001 cagtacttcg taatattgtt caaatcattg aacttatcaa taatcgagct ggagaagcgg
     9061 cttaaattct aaagtgattg ccatgctgag actcatttaa aattaaaaag agtagaaatt
     9121 tatgctatat atgagaagtt ttattaggaa gaatgtcatc gttttcctag aatacagtat
     9181 cagttgttaa gtggttgata aatttcaaag tagatacttg taccacgatg tttgttgatc
     9241 gagttattaa caaaagagct actttgattt taaagaaata gaaaacaaaa agccgagcaa
     9301 gaattcaatt gcaggagaaa atgaaataat actcaatgaa aatcaaagag caaactagga
     9361 aactagctgc aggctgctca aaacactgtt ttgaggttgc agatggaagc tgacgcggat
     9421 tgaagagatt ttcgaagagt ataaatcttc ctaggataaa gcaaaacgca tagtatcaag
     9481 ggttttcaac acttgatact atgcgttttc tgatgttaaa gactttctac caggtttttt
     9541 aaaagcataa ttgttagttg tagtcattta ttattcttca aagaaaaatg gtggggcgaa
     9601 ttttttcagt tcttcaaagc acttttgagc agtatctgca tcttcacaga tgataagaca
     9661 gacatcatta ccacaaaggg tagcgatagc gtcagggaag ctcaaagtat caatgataga
     9721 accaaaggat tgagccagtc caggaagggt ttttagtagg acttggtgtt gaactgggcg
     9781 catccagaca agggcgtctt ccatgtagag ttcgagacgt ttttcccatt ttgagatgga
     9841 accattgtta agaacataat aagcgctatc ttcttcgcgg acttttgata ggttcatatt
     9901 tttgatgtcg cgtgagaggg ttgcctgggt tacttgaatg tcgttctcag caagaagggc
     9961 ttgcaactca gcctgtgtat gaatcttgtt ttttgtgata agagcgcgta taagttggtg
    10021 gcggtgttct gatttattca taataatgta actcctttta gcaaggtaag gtaagcatgg
    10081 actgagcgag gtcgacagtc aagtggtagt ctgtattgtc acggatggtg atttcaaagt
    10141 cagtagtata gaggactaaa cggagagtgt ctccttcttt tagcttgtaa atagttggct
    10201 gcagttcaaa ttgaacgtcc atccattcat ctgcagtaat atcctctact aacagtaaat
    10261 catttctatt ttgtaaatta aggtaacctt ttgtcacgac tcgttgtgcc tctggtctaa
    10321 atggcaattc acagagattt tccaacatgt gatagcgacc gttgtcaatg gttctagcac
    10381 ttaaaatagc tggataaggt tgtaggtatt tcttttgccc aaattctagc agttgggcag
    10441 ataagagccc cttgtttgta ctggatttga tacgaagatt gagctgagcg cgaccgttta
    10501 ggtggagatc tttagtcaca ggaaggttaa tagtaatctg attggctttc ccttgataga
    10561 gctctgtatt gaaggtttgg tatgtcttac catagcgctc aaaatcctta tctgggtact
    10621 ggttttgaat agcttgctct tcttgaccaa gtgagaaggt ttcacagttt tcttgcccac
    10681 cgaagttatc aagtgataac caagtctgtg gagctgtatt gtcctgccag ataacagtag
    10741 gaagttgaaa gtctgtttcc tgtcctagta atttcttggt caataaggca tttatggact
    10801 cacggaagtc aattgattgc caattgttca tgtaaacatg ggcaccatta tggaaaaaga
    10861 gatgcttgtg tatatgagta ggaagagcat ggaacatctg gtaaacatga agtggtttga
    10921 cattccaatc ctgagaacca tgagtaaaga caacctctgc ctttacttta tgggcattga
    10981 gcagataatt gcggtcatgc caaaactgat tgtagtcccc agtttttcgg tctagctgag
    11041 ctttcacttt ttctaagtca gcttggtgag cttcattgcc acggatatag tcgccagcta
    11101 agagattacg agaataggtt aactcagcaa gggagtcaaa gtcctcacct ggataaccac
    11161 ctgggctagt caccagaccg ttttcacggt agtagttgta ccatgatgaa attcctgcct
    11221 cggcaatgat aacttctaaa ccatcgactc ctgtagtcgc aagaccattg gacatggtac
    11281 ctagatagga aagtcctgtt gtagcaactt ttccgtttga ccaatcagcc ttgacttgac
    11341 gctggcgcgt gtgatcagta aaggcacggc aacgaccgtt aagccaatcg atgacatttt
    11401 tataagcctc gatttgctgg tagtctccat tagtcatgaa acctgtcgag tctttggtac
    11461 caacacctga gacatagaga ttggcaaagc ctctcggaag gaagtagtcg tttagtgtat
    11521 agctagagtt gatgtgagtt agcttttcct cagcctctgc tataagctca gctttacctt
    11581 ggggttggac gagatttagt tgaggtttct ctagctcaat cttgtgagga agcttaacct
    11641 caagctcgcc ctccatcttg tagagagcct tgtcactagc cttgtcattg gttccctgat
    11701 gataagggct ggctgtcatg atggcaggga tttttccatc aaaacgaggg cgaataatgc
    11761 taacctttac taggtctgat agcccttttt ggtcagtatc gacacgagac tcaacgtaaa
    11821 cgacttcacg aatgacatcc tggttagaaa aagtagccaa actcttgccg ttaaagtagt
    11881 ggtagtcatt atcctccgga ataagaccat cactaacaag ttggtcgata agagtatttc
    11941 cttttttggt gcgagtattg agtaactgat agagattttc aatcaagtca ccatatataa
    12001 tgggaaatcc agtttcttta cgaaaaacgt cactatcttc gaagtcaacc aaataagaaa
    12061 agcctaaaag ttgaaaagca acagtataaa aaatatctgc tgtcagttca tcttctgatt
    12121 gaaaaaatgt cagcaggtct gtttttttat cagctgctag gatagaaagt gggtagttgg
    12181 tgtcttgata agtgaaaaag aaacgacgta aaaaggtttc aagtgagtct ttgtgattgg
    12241 ctgtattttg taaatcaaag ccacattttt ttagttcaga taagacattt tcttttggaa
    12301 aattgatata actatattga ttaaaacgca tagaacctcc atatagaatg acagttaagg
    12361 ttattatatc aaaaaaaaag cagaaaggga attgttaact tcaaaaggaa ataatccaat
    12421 aaaaatgaat aaagtactaa attcaatata gagaacagag taacaataag aataaataga
    12481 tagggtataa aagttctagg agatttatat tatatgcttt ctatttttat atacaatata
    12541 gtataaatat aaaaatgatg acaaaaatac aaatgaatag aaaataaatt agtaagctga
    12601 tgaaattttt ctcaagagaa gccatttata ggtgaaaatg gtataatata gtgagaagga
    12661 tagaggagaa gtgtaaattg atcgcacaac tagatacaaa aacagtctat agttttatgg
    12721 aaagcgtcat ttcgatcgaa aagtatgtga gagcagctaa agaatacggc tacactcatt
    12781 tggctatgat ggatattgac aatctttatg gcgctttcga ctttctagag attacaaaaa
    12841 aatacggcat tcatcctttg ctagggcttg aaatgacagt gtttgtagat gatcagggag
    12901 tgaatttgcg ctttttagct ctatctagtg tgggctatca gcagttgatg aagctttcga
    12961 cagccaagat gcagggggag aaaacttggt cagtcctgtc ccagtacctg gaggatatcg
    13021 cggtcattgt gccttatttt gatagagttg agtcgttaga actaggctgt gattactata
    13081 taggggttta tccagaaaca ctagcaagcg aatttcatca tcctatctta cctctttatc
    13141 gggtcaacgc ttttgaaagc agggatagag aagttcttca agttttaaca gcgattaaag
    13201 aaaatctacc gctcagagaa gttcccttgc gttcgagaca agatgtcttt atatcagcaa
    13261 gttctttaga gaaactattc caagagcgtt ttccgcaagc tttggacaat ttagaaaagc
    13321 ttatttcagg catttcttac gacttggata ctagtctgaa actgcctcgt tttaatccag
    13381 ctagaccagc agtagaggag ttgagagagc gtgctgaact ggggcttgtt cagaaggggt
    13441 tgactagtaa agaatatcaa gatagactag accaagaatt gtctgttatt catgatatgg
    13501 gctttgatga ttatttcttg gttgtttggg atttgttgcg ttttggacaa tcgaatggct
    13561 attatatggg aatgggaagg ggttctgcag taggcagttt ggtttcttat gccttagaca
    13621 tcacggggat tgacccagta gagaaaaatc tgatttttga acgctttctt aatcgtgaac
    13681 gctataccat gcctgatatt gatattgata tcccagatat ttatcgtcca gattttatca
    13741 gatatgttgg taataaatat ggtagtaaac atgcggcaca aatcgttact ttttcaacct
    13801 ttggagccaa gcaagctctt cgagatgtct tgaaacgctt tggtgtgcca gagtatgaat
    13861 tatctgcaat tactaagaaa atcagttttc gtgacaatct taagtcggcc tatgagggaa
    13921 atctccagtt tcgtcagcaa atcaatagta agttagaata ccaaaaagct tttgagattg
    13981 cttgcaagat agagggctat ccaaggcaaa cctctgtcca tgcggctggt gttgtaatta
    14041 gtgaccaaga tttaaccaac tacattcctc taaagtatgg tgatgaaatt ccactgactc
    14101 agtatgatgc tcatggagtt gaggctagcg gacttttgaa gatggacttt ctgggactac
    14161 gaaatttgac ctttgtccag aagatgcaag agttgcttgc tgaaacagaa ggtattcatc
    14221 tgaaaattga agaaatcgat ttagaagaca aagaaacgtt agctttattt gcctctggta
    14281 atacaaaagg tatctttcaa tttgagcaac caggtgccat tcgtctgctt aagcgtgtgc
    14341 aaccagtctg ttttgaagat gtcgtcgcga ctacttctct aaatcgaccg ggtgctagtg
    14401 actatatcaa taattttgtg gcaagaaagc atgggcagga agaagtgact gttctggatc
    14461 cagtactgga ggatattttg gctccaacct acggcataat gctctatcag gagcaggtta
    14521 tgcaggttgc ccagcgactt gccggattta gtcttgggaa agccgatatt ttgcgtcggg
    14581 ctatggggaa aaaggatgcc tctgccatgc atgagatgag ggcttccttt attcaaggtt
    14641 cattagaagc tggtcatact gtggaaaaag cagagcaggt ctttgatgtt atggagaagt
    14701 ttgcaggtta tggttttaac aggtcacacg cctatgccta ctcagccttg gccttccagt
    14761 tggcttattt caaaacgcat tatccagcca ttttttatca ggtcatgtta aattcttcca
    14821 acagtgatta cttaatagat gcacttgaag caggttttga agtagcctct ctatccatca
    14881 acaccattcc ctatcacgat aaaattgcca acaaggccat ctatctaggt ttgaaatcca
    14941 ttaaaggagt cagtaatgat ttagctctct ggattattga aaatagacct tattctaaca
    15001 ttgaagattt tatagctaaa ttacctgaga attatctgaa acttcctctg ctagaacctt
    15061 tggtaaaagt tggtcttttc gattcatttg aaaaaaatcg tcaaaaagta tttaataact
    15121 tagctaatct atttgaattt gtgaaagagt tgggaagttt gtttggagat gctatttata
    15181 gttggcagga atcggaagat tggacggaac aagaaaaatt ttatatggaa caagagcttt
    15241 tagggatagg tgtcagcaaa catccactac aagctattgc aagtaaggct atttacccga
    15301 ttaccccaat cggaaatttg tcagaaaata gctatgctat tatcttggtt gaagttcaga
    15361 aaataaaagt gattcgtacc aaaaagggtg aaaatatggc cttcttacag gcagatgata
    15421 gtaagaaaaa attggatgtc actctctttt cagacttata tcgtcaggtt ggacaggaaa
    15481 taaaagaggg agccttctac tatgtaaaag gaaaaataca atcacgtgat ggccgtctgc
    15541 aaatgattgc acaagaaata agagaagcag ttgctgaacg cttttggata caggtgaaaa
    15601 atcatgaatc ggatcaagaa atttcacgca ttttagaaca atttaaaggc ccaatcccag
    15661 tcatcatccg gtatgaagag gaacagaaaa ccatcgtttc tccccatcat tttgtagcta
    15721 aatccaatga attagaggag aaattgaatg aaatcgttat gaaaacgatt tatcgctaaa
    15781 aatacggaaa atagaagaat tttcaacgta aatgtggtat aatcagtaag aatgttaaaa
    15841 gaaaaaggag cataaccaat atgaaacgta ttgctgtttt gactagtggt ggagacgccc
    15901 ctggtatgaa cgctgccatc cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag
    15961 tttttggtat ctatgacgga tatgctggta tggttgccgg tgaaattcat cccctagatg
    16021 cagcttcagt aggggacatc atttctcgtg gtggtacttt ccttcactca gctcgttacc
    16081 cagagttcgc tcaacttgaa gggcaactta aagggattga gcaattgaaa aaacacggaa
    16141 ttgaaggtgt agttgttatc ggtggtgacg gatcttacca cggcgctatg cgtttgactg
    16201 aacatggctt cccagctatt ggtcttccag gtacaatcga taacgatatc gttggtactg
    16261 actttacaat cggttttgac acagcggtta ctactgccat ggacgctatc gataagattc
    16321 gtgatacatc atcaagtcac cgtcgtactt ttgtaatcga agttatggga cgtaacgctg
    16381 gtgatatcgc tctttgggct ggtattgcaa ctggtgctga tgaaatcatc atccctgaag
    16441 caggcttcaa gatggaagat atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa
    16501 aacacaatat tatcgtctta gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac
    16561 ttaaagaagc tggagataca agcgaccttc gtgtaacaga acttggacat attcaacgtg
    16621 gtggttctcc aactgcgcgt gaccgtgttt tggcgtcacg tatgggtgca catgctgtta
    16681 aacttcttaa agaaggtatc ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg
    16741 aaaatccaat tcttggtact gcagaagaag gggcattgtt tagccttact gcagaaggta
    16801 agattgtggt taacaaccca cacaaagccg atattgagct atctagcttg aataagagct
    16861 tgtcataatt tacaatttag ttaataagac caaaaaggtc atatatataa aggagtcaca
    16921 aaaatcatga acaaacgtgt aaaaatcgtt gcaactttgg gtcctgcggt agaaatccgt
    16981 ggtggtaaaa aattcggtga ggacggatac tggggtgaaa aacttgatgt tgaagcttca
    17041 gctaaaaaca ttgctaaatt gattgaagct ggtgctaaca cattccgatt caacttctca
    17101 cacggcgacc accaagaaca aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt
    17161 gcaggtaaaa aagttggttt ccttcttgat acaaaaggac ctgaaatccg tacagaattg
    17221 ttcgaaggtg aagctaaaga atattcatac aaaactggtg aaaaaattcg tgttgcaact
    17281 aaacaaggaa tcaaatcaac tcgtgaagtg attgcgttga acgttgctgg tgctcttgat
    17341 atctatgatg atgttgaagt tggtcgtcaa gttttggttg acgatggtaa acttggtctt
    17401 cgtgtggttg ctaaagatga tgcaactcgt gaatttgaag ttgaagttga aaacgatggt
    17461 atcatcgcta aacaaaaagg tgtgaacatc cctaacacta aaattccttt cccagctctt
    17521 gctgaacgcg ataacgacga tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca
    17581 atttcattcg tacgtactgc aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact
    17641 ggaaacggac atgttcaatt gttcgctaaa atcgaaaacc aacaaggtat cgataactta
    17701 gatgaaatca tcgaagcagc tgatggtatt atgattgctc gtggtgatat gggtatcgaa
    17761 gtaccgttcg aaatggttcc agtttatcaa aaaatgatta tcaagaaagt caatgctgca
    17821 ggtaaagttg ttatcactgc aacaaacatg cttgaaacaa tgactgaaaa accacgtgca
    17881 actcgttcag aagtatcaga tgtattcaac gctgttatcg acggaactga cgctacaatg
    17941 ttgtcaggcg agtctgcaaa cggtaaatac ccactcgagt cagtaactac aatggctaca
    18001 atcgacaaga acgctcaagc tcttcttaat gaatacggac gtcttgattc agattcattt
    18061 gagcgtaact ctaagacaga agtaatggct tctgctgtta aagatgctac tagctcaatg
    18121 gatatcaaat tggttgtaac tcttactaag acaggtcata ctgcacgttt gatttctaaa
    18181 taccgtccaa atgctgacat cttagcattg acatttgacg aattgacaga acgtggcttg
    18241 atgttgaact ggggtgttat cccaatgttg acagatgctc catcttcaac tgacgatatg
    18301 ttcgaaatcg ctgaacgtaa agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc
    18361 gttatcgttg ctggtgtgcc agtaggagaa gctgttcgca caaacacaat gcgtatccgc
    18421 acagtacgtt aagaaaaata taaaaaccta tcatatccag ctttagagct tgtgtgatag
    18481 gctttttgta tagagggtaa gaaataggca aaactttcat aatggattga tactcttcga
    18541 aaatctcttc aaaccacgtc agcgtcgcct taccgtatat atgttactga cttcgtcagt
    18601 tctatctaca acctcaaagc agtgctttga gcaacctgcg gctagcttcc tagtttgctc
    18661 tttgattttc attgagtatg aaataagata tgcacaaatt gattagaaag tcaaatgaat
    18721 ttctacaaat gttttagcaa tcgtaatgta cttgtctaga ttcgatctga tatattttcg
    18781 atttaatgat atggtattta aaacctccaa agtagcttac tccattcttt tacttacgtg
    18841 agtgtagatg ttatttactg ttttagcgtt tttgtgttcc actctaacca ttatagcatt
    18901 cttctcagct agtgtactaa ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt
    18961 tatcggtttc tctagtttag tatttgcctt ttgcaaagtg atcttaaatg cctttctcta
    19021 aatttacata tcactattgt ttaacaaaat ctaatctatt ttaggtcact tattcttttt
    19081 ttgaaatgta gaatgaactt tttcaaagtt tttcgaatct tttaaaatct gtttgcttta
    19141 tatcgccatt ctcccccctt ttttaattct ccctatatag cctgacagct ttcccgatgg
    19201 tacgaatatg gttgctttcg tctaggtgga tgtcggggta ttcgggattg agtttttttg
    19261 aggcagcctt ggcggagttt cttgacatag ttagtgccgt ctacttggaa gatgccgatg
    19321 gtattatagt caatctgtgg ggtattcttg ataaataggt agtcgctgtt tcttatcttt
    19381 ggctccatgg acttgctgac gacataagcg attgggtcgt agtcgtctgg gataatggaa
    19441 actccatatc taaatcgttg tcctgcatcg agcggctacc tgcagagata aactacctaa
    19501 cacgagagta agtagtctgt ctgtagtcgt ccagtctgat gatttttacg atacttcgtt
    19561 tttctgatca tacagttgcc tctcggcata ggtcagaact ttaccttgtc tgggtggttc
    19621 ccgttggtcg tagatagatt ggatatcgct aggagaatcc ttttgaactg gaggaaagag
    19681 ggcatcgatc aagctactga atactttaac taagtcaaat atagtatttt tcttagtaga
    19741 cctaaccctt ttttcataat ttctaatggt gtttttactt atacctatct tagtacccaa
    19801 ttcttattga gtccaaccat tactagtcta tattgtttta tagttgattg agtttggaat
    19861 agtacgctgt agctgctaaa acatttctag aaattaattt gactttccta atagagttgt
    19921 tcatatctta tttcaatcta ttatgttttt cacctctaac aatcgcaatc tcttctttat
    19981 ccatgaatga aatcgctttc tatttttgta agtaaagcat aacacgaaat ccacgaaaat
    20041 gaaaaccttt gttgtgtttt cgtaaaaaat ttgttgacag agcacgaaac gcctactata
    20101 attaaatcaa gcttaaggaa ataacaaaaa aacaaatcgg agggaaagac gatgaacaca
    20161 ttaaatgaga aagtaatcaa tatctgtaaa gcaatagtta aagaaacttt aatccaagac
    20221 atttaggaga aagaaatgaa gaaatcaaga aaattagcca cattaggaat ctgttcagcc
    20281 ttgtttttag gcttggctgc ttgtcaacaa caacatgcta cttctgaggg gacgaatcaa
    20341 aggcaaagca gttcagcgaa agttccatgg aaagcttcat acaccaacct aaacaaccag
    20401 gtaagtacag aagaggtcaa atctctctta tcagctcact tggatccaaa tagtgttgat
    20461 gcatttttta atctcgttaa tgactataat accattgtcg gctcaactgg cttatcagga
    20521 gatttcactt cctttactca caccgaatac gatgttgaga aaatcagtca tctctggaat
    20581 caaaagaagg gcgattttgt tgggaccaac tgccgtatca atagttattg tcttttgaaa
    20641 aattcagtca ccattccaaa gcttgaaaag aatgaccagt tgcttttcct agataatgat
    20701 gcgattgata aaggaaaggt ctttgattca caagataagg aagagtttga tattctattt
    20761 tcgagagttc caactgagtc aactacagat gtcaaggttc acgctgaaaa gatggaagca
    20821 ttcttctcac aatttcaatt caatgaaaaa gctcgaatgc tgtctgtagt cttgcacgac
    20881 aatttggatg gcgagtatct gtttgtaggc cacgttgggg tcttagtacc tgctgatgac
    20941 ggtttcttat ttgtagagaa attgactttc gaagagccct accaagcgat taaatttgct
    21001 agtaaggaag attgctacaa gtatttgggc accaagtatg cggattatac aggcgaggga
    21061 ctggctaagc cttttatcat ggataatgat aagtgggtta aactttaata gtgttatgat
    21121 atgacagtaa tcttaggatt tttatttcta tttttgataa gttggtgggt tatttcaatc
    21181 gttaatagcc agaaaccacg caaaaatgaa acatttattg gctatgtcca aagatacgat
    21241 attgatggga atggctatca atcattaccg tgtcctcaga actgagagga gttcttgaaa
    21301 tcgtaacacc actttgaaca agagttactt aaacccattg gctccgacgg attaagttgc
    21361 tttcgtgaat accaaaatca gccgcaattt cttcataagt gcggtattct cgcacatatt
    21421 gaagagtggc cataagaagg tcttctaggc ttaatttagg ttttcgtcca ccttttgcgt
    21481 gtttaagttg ataagctgtt tttaatacag ctaatatctc ttcaaaagtc gtgcgctgaa
    21541 caccaacaag acgtttaaat cgtgcatcag ttagttgttt acttgcttca taattcatag
    21601 aactactata ccatattttg tttcgcagga agtctaatgc taaaatctta tcagtgagaa
    21661 gatttcttaa agtttattct ttgtagtttc atctgtatta gtacataatt taacttagag
    21721 aaaataggag gactaatcga atggaattaa aagattttac agaaaaagaa caggaaatga
    21781 ttaagaaaag gcttacaatg tctaatatta gtgacaaaga aactactgag aagattctgg
    21841 cgctagtccc acaagacttg attaagcgaa tcccgttttt tgtcagaaaa catgctacaa
    21901 cacgtacgat taaacgcatt tcaattgaat accctgaact ctacgctgta gctcaaacaa
    21961 gtggtgagat tccagaaaaa aaacgcgaag aattgcgtca gattatcaca actatctttg
    22021 aacaaaagat gaataagcat agtattaagt agagaatgaa aaaatatttt attggcggtt
    22081 tgggaagcaa tgcctatcat agcaaggatt ttcttcaaga actagattcg caggtctatt
    22141 ttctaaatcc atatgaaaag catcttcgag atgaaacaga attgaaatca tggtttaaaa
    22201 atgaaattgt agaggaagaa tctatctgtc tgataggcca ttctcttgga ggagatttag
    22261 ctcgttattt cgcatcggaa tttgaagaag taaagaaact gattcttttg gatggtggct
    22321 atctagattt agataagatt ttacctttgg atacagagtt agaggaaact aaaaattata
    22381 tcaaatctca aattgtttcg gacttagatg ttcttacttc taaagaaaaa tctgaagcaa
    22441 agcattggtc agaaaatatg gagaaagctg taagacagtc ttatcactgg aatgttgagt
    22501 ataatagata tgagttggct ataaattatg aaaatataga agcgatactc cgcctacgga
    22561 gaaaaataca agcttttaag agagaagtgg gagatacctt gtttattagt cctcgctatc
    22621 ctaatgaagc tacatggaga gaggaagccc taaaagaatt gccagactat tttgatacta
    22681 tttttttaga aaactttggc catgagcttt atactcaagc acctaaagaa atcgctagtc
    22741 taatgaatga gtggctcgct tattttctat gagctacttg tattgctggg aatggtataa
    22801 gatgaaaatc tacaagtcac taattttcat ataacaaaaa acgaacaggc tctttttctg
    22861 ttattcagaa gagaacttgt tcgtttttat cttgattttc ttaaaaagct ctatatacat
    22921 agggattttc tggtttatcg actttggtaa agctgtcgat ttccaaggtt ggaaggtttt
    22981 gtttgagttc tttatggtag tgattgacca gttttccttt ggctgttatc caagtgttgt
    23041 tttcatactg ccgggtattg cccttggtca gcaatccata gacaccagaa tctgcgatac
    23101 agtggataat gccgaatcgg aacagaaatt gactattggc atgactgggg tcgttataga
    23161 caaagcctgt aaactggatt gtcttgccct caaactcatc tggatagtcg tagatagcct
    23221 ccatgacttc catatagttt tcattagtga tctgaatact atcttgggat aagtatttat
    23281 ccgccgccgt tcgcatttcc ttttcatagg ctgattttga aaaataagaa ctggtatctg
    23341 gtttcaaata ttggcttgtc gtcccttcgc ttgtctgaat ggctagatcc gttccttccg
    23401 atagggggaa atgataacct ttagcagaaa cagtctgaga atccaagcta acagttggga
    23461 aagttaagcc gatgacaagt ggaatagcca gaagagaaat actcgttatc ttggctaatc
    23521 ggctgttcag atgactgtgg gttttgactt gcttcatcca gatatacaat tgaacgatag
    23581 ccaagataaa agaaagcacc atggagatat aggccagata ggaatagtgc atgttgatgt
    23641 actggtttag tttgcccgac agatggaggt aaatagtcag ttcaaaatag ccagctaaaa
    23701 ctaaaaatcg aatcatagga taactccaat caagagagaa tagactaaga cgacaagagt
    23761 tacaattgtt atgaagtgac tgataaatcg tgctttcaag taatttttca tcatgagaat
    23821 atttttgata tccagcattg gaccaatgac gagaaaggcc agaactggtg ccaaaccgaa
    23881 actcgagaga agagaagcac ctataaaggc atccgcctca ctacagagcg aaagaagaaa
    23941 ggctaaaatc atcaagagca ggatggcaaa aagaggggtc gcactgatag aggtcagaat
    24001 ccgagtcgga acgtagacct gtattataga agcaaagagg cagccaaata ccaaataacg
    24061 ccccgtatca aaaaattcat caatggcctg cacaaagact tgaaaaactt tttttgcaga
    24121 actcaagtaa gaaaaatcat gctcatgaca agccagacga ttttctttct gaatcggttc
    24181 ttgccagaaa aatcctagaa aaattcctag tattacagcc acaagaatgg aacccagagc
    24241 tcgtaatagg gcgacatgga aggagttgcc aaaggcagaa taggtcgcaa aaagaacaat
    24301 gggattgata acaggtgctg tcacaagaaa aggaacggcc gtgtaacttg gaaccttttt
    24361 ttccagaaaa cgattgatga tggggacgat tccacattca caagaaggga aaagtatacc
    24421 gacaaaggtc ccaaaaaaga ttctccccca acgatttcga gggagaaaat gataaacctt
    24481 gtcaggtgtg atataaactt caatcagccc tgagacaatg cttcctatca gaacgaaggg
    24541 aagggcttca atgataatgg agagaaaaat ggctccagtt tgaagtacac tagatgggag
    24601 ttgttgaaaa gcagtcatct agtttttctt ttctgctttg acttttttct ctttgtcatc
    24661 agggaaagta acttgtttca aatcagggag tttagcaaac tcttcaaaca tcttgtccaa
    24721 gtcgtctcgt tttgtaaatt tgacagccat aggtgtacct cgattctaga ttctacctct
    24781 attatactat tattttgcgg aaaagggaaa agttaaaatt tcaaattgag tagctaagag
    24841 ataatatagc ttgatattac ctgatatata tcataattgt gctataatta aataaaaaga
    24901 aaatcattat cagaaaatta tgggaggtag tcaaactgac ttactcaatt agtgttgttt
    24961 tgttacatca aggagataaa aatagtttat tagagtcttt gtcaatagtg gagagacaaa
    25021 cattaaaaga tatagaagtt attattgtaa atcaatctac agaggagata gcttccatta
    25081 gccaacagat gaatctatca tttaatatta agattataga tgagaattct tttgctgagt
    25141 tgtctgatat gattacagga gattatatta cgtttctatc ttcaaatgat tcattatttg
    25201 attggacatt tgaaaagatg tatcatgcga taaaattatc cgattctgat gttgttcttg
    25261 gtcattttgc tgatttggtt gatggagtat tttactttta tcctttttgg ggggataatt
    25321 ggttgacaga aaatttaacg aatgttcaag ttttacaaaa gatggatgat acagatcatc
    25381 gaattagaaa acaatatcgc tcattatttg gaaaattatt caatagtaag cttttacgaa
    25441 aaataaatca atttgatata aacaacctta tttggagact ttatattcac tctaaaactg
    25501 caacctatat taatttcccg acatatatct ataaacctgt tatagatgct aaacctctaa
    25561 aagactccta caaaatcata ttagaaaatt atgaaaatag aatcaaagat tgcgccgtct
    25621 tagaaaacta tgatatagaa agagcgaaaa aacaatatat tcaagaattg gcaaatttta
    25681 gtgcttggtt aaaaaatgac ggtgaatatt tagattctga tattgtttat cataagttaa
    25741 tagctgcaga gaaaggaatt ttcccttttc ttgagcttga tagattagat tttacgatta
    25801 taagtaataa ttgtgttgga ggattaattt ataaacagct tggttttcaa tatagaacac
    25861 cttttgttgg tttatttatt cttcctgatg attattataa acttacaaag cattttcgtt
    25921 attatatgga acaagaactt gttttcgaag aggaacttat ttcaccgagc tggactcatg
    25981 aagtttatcc tttggggcac ttaggagata tagatattca ttttctacat tattctagta
    26041 ttgaagaagc acgaactaag tgggaaaaaa gaaaaaaacg cataaattgg aataatcttt
    26101 attttaaatt tgataataaa gattcagcta gtgaagaaat tttaaataag atggataatc
    26161 ttccttattc taataaatta attttagtca atcgtccata taaaaatttg aaaagtcaat
    26221 gtgttattcc aggtcaggaa catcttccag aactggctat tatgtctgat ccattaaata
    26281 cttttgatat catggcttgg ttaaaaaaag gtggcaatgc tctttaagtg caagttattg
    26341 cgctgtagca tataaatcta tttcctacat attttttaaa tgttctacga gttaatttga
    26401 aacgtttagc ttgtggtata atagatttat ggataaaaaa tatgaaaaaa tctctcagga
    26461 tttgggagtg acgttaaagc aaattgatac cgttctaagt ttgacagctg aaggggcgac
    26521 tattcccttt atcgcgcgtt atcgcaagga catgactggt agtctggatg aggtggcgat
    26581 taaggctatt attgatttgg ataaaagtct gacaaatctc aatgaccgta aggaagctgt
    26641 cttagctaag attcaagaac aaggtaagtt gaccaaggaa ttggaagaag ctatcttagt
    26701 tgccgaaaaa ttagcagacg ttgaagaact ctatcttcct tataaggaaa agcgtcgtac
    26761 caaggcaacc attgcccgtg aagctggact ctttcctctt gctcgcttga ttttgcagaa
    26821 tatagttgac ttagagaaag aagctgaaaa gttcgtctgt gaaggatttg cgactggcaa
    26881 ggaagccttg accggtgcag ttgatatttt ggtcgaagcc ttatcggaag atgtgacctt
    26941 gcgttctatg acttatcagg aagtgctgag acactctaaa cttacttctc aagccaagga
    27001 tgaaagtctt gatgaaaagc aggtttttca gatttattat gatttttcag agacagttgg
    27061 aactatgcaa ggctatcgta ccttggctct caatcgtggg gagaaacttg gtgtcttgaa
    27121 gatcggtttt gaacatgcga cggaccgtat tcttgccttc tttgctactc gtttcaaggt
    27181 gaaaaatgct tatattgatg aagttgttca gcaatccgtt aagaaaaagg tcttgcctgc
    27241 tatcgagcgt cgtattcgga cagaattaac tgagaaagct gaagaaggag ctatccaact
    27301 tttttctgac aatctgcgca atctcctctt ggttgctcca ctgaaagggc gcgtggttct
    27361 tggatttgac ccagcctttc gtacaggtgc caagctagct gtcgtggatg caacaggaaa
    27421 aatgctgaca actcaggtta tttatcctgt taaaccagca tcagctcgtc aaatcgaaga
    27481 agccaagaaa gatttagcag atttaattgg tcaatacggt gtagagatta ttgccattgg
    27541 aaatggaacg gccagtcgtg aaagtgaagc ttttgtagcg gaagttctga aagatttccc
    27601 tgaagtcagc tatgttatcg ttaatgaaag tggtgcttct gtctattctg ccagcgaact
    27661 tgctcgtcag gagtttccag acttgaccgt tgaaaaacgc tctgccattt ctatcgcccg
    27721 tcgtttgcaa gatcctcttg cggaattggt caaaatcgat cctaagtcaa ttggtgtcgg
    27781 tcaataccaa cacgatgtca gtcagaagaa actatctgag agtctggact ttgttgtcga
    27841 tacagtggtt aaccaagttg gtgtcaatgt caatacagct agcccagctc ttctttcaca
    27901 cgtagctgga ctcaacaaaa ctatctctga aaatattgtc aaataccgcg aggaagaagg
    27961 aaaaatcact tcacgcgccc aaatcaagaa agttcctcgt ctgggagcca aggcctttga
    28021 gcaggctgct ggtttccttc gtatccctga aagtagcaat atccttgata atacaggagt
    28081 tcacccagag aattacactg cagttaagga actcttcaaa cgtttagata ttaaagacct
    28141 aaacgaagaa gcccaaagca aactcaagtc gctttcagtc aaggaaatgg cgcaagagtt
    28201 agaccttggt ccagaaactc ttaaagatat cattgcagat cttctcaaac caggtcgaga
    28261 tttccgtgat tcctttgacg cacctgtgct tcgccaagat gtcctagata tcaaggactt
    28321 agtggttgga caaaagctag aaggagtggt gcgtaacgtt gttgatttcg gtgcttttgt
    28381 tgatatcgga attcatgagg acggtctcat ccatatttcc catatgagtc gcaagtttat
    28441 caaacaccct agtcaagtgg tggcagttgg agatttggtg agcgtttggg ttaagaaaat
    28501 tgatactgaa cgtgaaaaag ttaatctgtc tctcctagct ccaaatgaaa ctgactgatt
    28561 acgtcaagca ggtttcacta gaagacttcg gcagaccttt tatccatcat gtccagtgga
    28621 ataggcgtct acgttcgaca ggtgggcgat ttttccccaa agatgggcat ttggatttta
    28681 atcccaaggt ttatcaggaa ctggggttgg acgtttttag gaaaattgtc cgacatgaac
    28741 tctgtcatta tcacctctat tttcaaggga aaggctatca acacaaggat cgggatttta
    28801 aggaactgtt gaaagcagtg gatggattac ggtttgtacc atccttgccc aatagtaact
    28861 ccaagccaat caagctctat cgttgccaat cctgccagca aaggtatcag cgtaagcgta
    28921 ggattgatac ccaacgctat cgctgtggac tttgtcgagg aaagttgcta ttagtaaatc
    28981 agcctgagga ctgatgaaag ccgagcctat ccgtgatata ctagtctaac ctagcagaaa
    29041 gaggaaatat catgaacagt aagttttata aaatgagacg aaatcgcatg atttcaggag
    29101 ttttagcagg tctgtcagac aagtggaact ttgatgttac ccttgtacgt tttctgtttg
    29161 ccatttttac agtcgcaaac tttggacttg gggtgattat ctatatcatc ctagcttcta
    29221 tcatgccaac caaggaagaa atcgaagcag aaatgtacgg aacaggccca cgtaaacgca
    29281 aagaagctca agccattgac gataatgaag gctggttttg gtgaggtttt acaaaagatt
    29341 tggaatacgc aaaaagcttt aaatcaatag tctaaactgc ttgatttaaa gcttttttac
    29401 ttttatactc aataaaaatc aaagtgcaaa ctaggaagcc agccgcaggc tgtaatttag
    29461 tcggcaatgt gaagttgata tagtttgcac tttgattttt gaatagtatt aataatattt
    29521 cgccagcttc atccacaaac tatagtggat tggaactaga ataggacacc tatacttcta
    29581 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
    29641 tactataaaa gcagtaattt aaacgcagtt ttttaatcta tttttgctgt tgagacaata
    29701 catttttgga aaaatggtgg atgctgataa caactgataa ccagcaaata acataagcag
    29761 tgccggtaaa ttctgtactg ataaagtgtg tgtgttcaac taagagctga ccgatgaatg
    29821 gagagagcag atagagaagt ccgatgagta tatgttcttt gtgtttcatt aaagcgcacc
    29881 ttctttcctt ataggttgaa cgacttgcaa gtgttaaatt gaatggtatg ttatctaggt
    29941 aagcttacta aaatgttagt tcccgcttcc tccttttcaa agtaagcctt ttcacactta
    30001 tattttacaa ctttaaaaat aggaaatcaa atcattttct aagtaatttt gcttatattt
    30061 cacccaatct tacaaaaatg taagattaaa gtctcttttg taaggttagg ttatggcaag
    30121 cggtcttttt ttgatgtaaa ctagactcat aaagaacaaa ggagtaacat catgaaacaa
    30181 atcttacata agaaaactag aaaaccaagt cacaaagaaa ttgagcgcgt tcagctggga
    30241 tgcactatga tgcaagctac atttcatttg atgggatatt aagaaaggag attttcatga
    30301 cacttttaga tgtaaaacac gttcaaaaaa tttataaaac acgttttcag ggcaaccaag
    30361 tagaagccct caaggatatt cactttaccg tagaaaaggg tgactacgtt gccatcatgg
    30421 gtgagtctgg ttctggtaaa tcaactcttc tcaatattct agctatgttg gataaaccaa
    30481 gtcgtggtca ggtttacttg aatggaactg acaccgcaac tattaaaaat tcacaggctt
    30541 ctagtttccg gcgtgaaaag ctaggatttg tcttccaaga ctttaacttg ctagatactc
    30601 tgtctgttaa ggacaatatc ttgcttccgc ttgtcttgtc aagaagacct ataacggaga
    30661 tgatgaagaa attggtggtg acagctgaga atctgggtat taaccaattg caagagaagt
    30721 acccttacga gatttctggt ggtcagaaac agcgtgtagc agtagcccgc gccatcatca
    30781 cagaacctga aattctcctt gcggacgagc caacaggagc ccttgattcc aagtcatctg
    30841 cagccttact tgatgtcttt aatgaaatca atgagcgtgg gcaaaccatc ctcatggtaa
    30901 cccactcaac agcagctgct agcagggcca agcgtgttct ctttatcaaa gacggcattc
    30961 tttacaacca aatctaccgt ggagagaaga cagagcgtca gatgttccaa gaaatctctg
    31021 ataccttgac tgtcatggca agcgaggtga attagtatgt ttcgattaac caataagtta
    31081 gcggtatcga acttgattaa aaaccgcaaa ctctactatc cctttgcact ggctgttctc
    31141 ttggcagtca ccatcaccta tctcttttac tccctaacct tcaatccaaa gattgcggaa
    31201 atccgtggag gaaccaccat tcaagcaaca cttggatttg gtatgtttgt cgttaccctt
    31261 gcgtcagcca ttatcgtcct ctatgccaat agttttgtca tgaaaaaccg ttccaaggaa
    31321 ctgggtatat atggcatgtt aggcttggag aagcgccatc taatcagtat gacctttaag
    31381 gagttagtgg tatttgggat tctaactgtt ggagcgggta tcggtattgg agccttgttt
    31441 gacaagttaa ttttcgcttt cctgctcaaa ctaatgaaac tgaaggttga gctggttgct
    31501 accttccaaa tgaatgttgt cattgcagta cttgttgtct ttggattgat tttcctaggc
    31561 ctcatgttcc tgaatgctct tcgaatcgcc cgtatgaatg ccctccagct ctcgcgtgag
    31621 aaagcaagcg gagagaaaag aggtcgcttc ctacctctcc aaacgattct tggttccata
    31681 agtttaggga ttggctatta tcttgccctt acggtaaccg atcctcttac agccctaaca
    31741 actttcttcc tagctgtttt gctggttatc tttggtactt atctattgtt taatgcaggg
    31801 attacagtct tcctacaaat cttaaagaaa aacaagaaat actattacca acctaataac
    31861 ctcatatctg tttccaactt gattttccgt atgaagaaaa atgcggttgg actagcaacc
    31921 atcgctattt tgtcaacaat ggttttggta accatgtcag cagcgacaag cattttcaat
    31981 tccgcagaaa gctttaaaaa agttctaaat cctcatgatt ttggggtttc agggcaaaat
    32041 gttgaaaaag aagatttgga caaactcttg agccagtttg caagtgacaa aggttatagt
    32101 gtcaaagaga aagaagtact tcgttacagt aactttggta ttgcaaatca agaaggaacc
    32161 aagttaacta tttttgaaaa aggacaaaac cgtgtccaac ccacaacagt tttcatggta
    32221 tttgaccaaa aagattatga aaatatgact ggtcaaaaac tgtctctatc aggaaatgag
    32281 gtcggtctct ttgccaaaaa tgacggactg aaaggacaga aagctctaac tctaaatgat
    32341 catcaatttt ctgtcaaaga agaatttaat aaagatttca ttgtgaacca tgttccaaat
    32401 aagtttaata tcttgactac tgattacaat taccttgttg ttcctgattt acaagccttt
    32461 ttggatcaat tcccagattc ggctatctat aatcagtttt acggtggtat gaatgtaaat
    32521 gtcagtgaag aagaacaact caaggtcgct gaggagtatg aaaactacct caatcaattt
    32581 aatgctcaat tagacacaga aggtagctat gtttatggta gcaatctagc agatgctagt
    32641 tctcagatga gtgccctctt tggtggtgtc ttctttatcg gtattttcct atccattatc
    32701 tttatggtcg gaactgttct ggtcatctac tacaaacaaa tttctgaagg ctacgaagac
    32761 cgtgaacgct ttattatctt gcagaaagtc ggtttggacc aaaagcaaat caagcaaacc
    32821 atcaacaaac aggttttaac tgttttcttc cttcctttgc tctttgcctt catacatctc
    32881 gcctttgcct accatatgct tagcctgatt ttaaaagtga ttggtgtact ggatacgact
    32941 atgatgttga ttgtgacctt gtctatctgc gctatcttcc tcatcgccta tgtgctgatt
    33001 ttcatgatta cttcaagaag ttatcgcaag attgtgcaaa tgtaaaaaag atacctcgac
    33061 ttcaaaatcg aggtatttct tgtattctaa atgctgaaaa gttgtccgag caggaaggta
    33121 actcccatgg tcaagagacc aatagcaagg ttccgaatca tagctgtttt ggttggggct
    33181 tttccaagtc tagcacttgt gtaaccagtg agaagaaggg ccacaccgac aataaggacg
    33241 gtagcaggga tgcggtaatc acttggaaaa atggtcactg acagcattgg aggcaaactt
    33301 ctaaggaaaa aggcaacgaa gctagaaatg gcagcgtgcc aaggattggt aaattcttca
    33361 tactcaatcc catatttttc ctctaccaga gccttgagtg gatttttaag aaagatctta
    33421 ttggtcaaga gttgggcaga agttttgaat tctccatttt ggatataagc agcatagagg
    33481 gattttttgg ctagttccct atcttggtct agcaagagtt tttctcgcga aacggcagct
    33541 tcctcggtat cttttggagt tgaaacggat acatattctc caccagccat tgaaaaggca
    33601 ccagctaaga tagccgtaaa acctgataaa aagataatcc agatattggt cgtggcactg
    33661 gcaactccga taaccacacc agcaatggaa ataattccat cgttagcatc aagaacaccc
    33721 gcacgcagga tatttaaacg acctgcaaaa tttgaatcaa tttcgtgatt tgtttctgac
    33781 gctaaatttc aagttcaagt tagccatc
//
LOCUS       DBDESG010000019        32880 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.019, whole genome shotgun sequence.
ACCESSION   DBDESG010000019 DBDESG010000000
VERSION     DBDESG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.019"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..254
                     /locus_tag="ACKB38_000874"
     CDS             <1..254
                     /locus_tag="ACKB38_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGL7979667.1"
                     /translation="RFLESKGIQASMSRKGNSPDNSMMESFFGILKSEMFYGYEKSFQ
                     SLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(382..1404)
                     /gene="cbpF"
                     /locus_tag="ACKB38_000875"
     CDS             complement(382..1404)
                     /gene="cbpF"
                     /locus_tag="ACKB38_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGL7979668.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1423..2244)
                     /gene="cbpG"
                     /locus_tag="ACKB38_000876"
     CDS             complement(1423..2244)
                     /gene="cbpG"
                     /locus_tag="ACKB38_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGL7979669.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2325..2564)
                     /locus_tag="ACKB38_000877"
     CDS             complement(2325..2564)
                     /locus_tag="ACKB38_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979670.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2714..3482)
                     /locus_tag="ACKB38_000878"
                     /pseudo
     CDS             complement(2714..3482)
                     /locus_tag="ACKB38_000878"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3483..4115)
                     /locus_tag="ACKB38_000879"
     CDS             complement(3483..4115)
                     /locus_tag="ACKB38_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGL7979671.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4129..5124)
                     /locus_tag="ACKB38_000880"
     CDS             complement(4129..5124)
                     /locus_tag="ACKB38_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGL7979672.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5121..5819)
                     /gene="liaF"
                     /locus_tag="ACKB38_000881"
     CDS             complement(5121..5819)
                     /gene="liaF"
                     /locus_tag="ACKB38_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGL7979673.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5896..6906)
                     /gene="fni"
                     /locus_tag="ACKB38_000882"
     CDS             complement(5896..6906)
                     /gene="fni"
                     /locus_tag="ACKB38_000882"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGL7979674.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6890..7897)
                     /locus_tag="ACKB38_000883"
     CDS             complement(6890..7897)
                     /locus_tag="ACKB38_000883"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGL7979675.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQTVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7884..8837)
                     /gene="mvaD"
                     /locus_tag="ACKB38_000884"
     CDS             complement(7884..8837)
                     /gene="mvaD"
                     /locus_tag="ACKB38_000884"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGL7979676.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(8819..9697)
                     /gene="mvk"
                     /locus_tag="ACKB38_000885"
     CDS             complement(8819..9697)
                     /gene="mvk"
                     /locus_tag="ACKB38_000885"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGL7979677.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9819..10150)
                     /locus_tag="ACKB38_000886"
                     /pseudo
     CDS             complement(9819..10150)
                     /locus_tag="ACKB38_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10762..11637
                     /locus_tag="ACKB38_000887"
     CDS             10762..11637
                     /locus_tag="ACKB38_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGL7979678.1"
                     /translation="MYPPELDSKILSIIKKAVTIRTTHTYEYQYSLLFGDAGYLWLLL
                     HLFSISKNQYYLQLANVTAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLK
                     IFIHNSIGEIYHYFLQRDTAKESILDYSFAHGYCGIAYALFAYSKVLEPSMFYNDLHT
                     FHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDIISKYQEFVFN
                     HHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKAD
                     LFDFGIGSMGYIGVY"
     gene            11680..12912
                     /locus_tag="ACKB38_000888"
     CDS             11680..12912
                     /locus_tag="ACKB38_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGL7979679.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(13030..14028)
                     /gene="cbpJ"
                     /locus_tag="ACKB38_000889"
     CDS             complement(13030..14028)
                     /gene="cbpJ"
                     /locus_tag="ACKB38_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGL7979680.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYLDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(14046..15068)
                     /gene="cbpC"
                     /locus_tag="ACKB38_000890"
     CDS             complement(14046..15068)
                     /gene="cbpC"
                     /locus_tag="ACKB38_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGL7979681.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            complement(15167..15856)
                     /locus_tag="ACKB38_000891"
     CDS             complement(15167..15856)
                     /locus_tag="ACKB38_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7979682.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(15868..17292)
                     /gene="gndA"
                     /locus_tag="ACKB38_000892"
     CDS             complement(15868..17292)
                     /gene="gndA"
                     /locus_tag="ACKB38_000892"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGL7979683.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(17368..18762)
                     /gene="mapZ"
                     /locus_tag="ACKB38_000893"
     CDS             complement(17368..18762)
                     /gene="mapZ"
                     /locus_tag="ACKB38_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGL7979684.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVESGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(18775..19932)
                     /locus_tag="ACKB38_000894"
     CDS             complement(18775..19932)
                     /locus_tag="ACKB38_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGL7979685.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(20017..20397)
                     /gene="rnpB"
                     /locus_tag="ACKB38_000895"
     ncRNA           complement(20017..20397)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACKB38_000895"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(20418..20747)
                     /gene="gpsB"
                     /locus_tag="ACKB38_000896"
     CDS             complement(20418..20747)
                     /gene="gpsB"
                     /locus_tag="ACKB38_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGL7979686.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(20817..21344)
                     /locus_tag="ACKB38_000897"
     CDS             complement(20817..21344)
                     /locus_tag="ACKB38_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGL7979687.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            21410..22006
                     /gene="recU"
                     /locus_tag="ACKB38_000898"
     CDS             21410..22006
                     /gene="recU"
                     /locus_tag="ACKB38_000898"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGL7979688.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            22003..24162
                     /gene="pbp1a"
                     /locus_tag="ACKB38_000899"
     CDS             22003..24162
                     /gene="pbp1a"
                     /locus_tag="ACKB38_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGL7979689.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(24329..29632)
                     /locus_tag="ACKB38_000900"
     CDS             complement(24329..29632)
                     /locus_tag="ACKB38_000900"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGL7979690.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVANKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTKNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDESKLLSSISF
                     LGNALVSVSSNQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGADVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HNPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALILASVSLALSALFVVKTKKD"
     gene            complement(29934..31916)
                     /locus_tag="ACKB38_000901"
     CDS             complement(29934..31916)
                     /locus_tag="ACKB38_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7979691.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            32121..32306
                     /locus_tag="ACKB38_000902"
     CDS             32121..32306
                     /locus_tag="ACKB38_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979692.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            32287..32610
                     /gene="tnpB"
                     /locus_tag="ACKB38_000903"
     CDS             32287..32610
                     /gene="tnpB"
                     /locus_tag="ACKB38_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGL7979693.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQI
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(32765..>32880)
                     /locus_tag="ACKB38_000904"
     CDS             complement(32765..>32880)
                     /locus_tag="ACKB38_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGL7979694.1"
                     /translation="TLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRVLFESR"
BASE COUNT         9430 a         7445 c         5845 g        10160 t
ORIGIN      
        1 atcggttcct agagagtaag ggaattcaag catccatgtc acgtaaggga aacagcccag
       61 acaatagtat gatggagtcc ttctttggga ttctgaaatc ggaaatgttt tacggttatg
      121 agaagtcgtt tcagtcgctt aagcaattgg aacaagccat tatagactat attgattact
      181 acaacaataa acgaattaag gtaaaactaa aaggacttag ccctgtgcaa tacagaacta
      241 aatccttcgg ataaattaat tgtctaactg tttgagggca gtacagaaaa accttgatac
      301 tatgcgtttt ttgattgtga agacttttat ccacctatta tacaaagtta agtatccatc
      361 acagtttaca attagccttc attacttaac ccattcacca ttatagttta agtagtaacc
      421 acctactgtg gtattaacag ctaaagcacc tgaatcatag gcatagtacc agttaccatt
      481 gacttggaac cagcctgttt tcatatctcc attacttgca tttagatagt accaagtcga
      541 actttcctga taccaaccag ttgccatagc tcctgatgaa cggagatagt accatttgtt
      601 cccaaggttt tgccagccag ttctcatatc accattttca gcatctagat agtaccaagt
      661 tgagccttcc ttataccagc cagttgccat agctcctgac gaatggaggt agtaccatct
      721 attacctaga tattgccaac ctgtttgcat aatgccagtt gctggattta gatagtacca
      781 tggagctgct tttagcttct cttcatcata cgtaagaggg taatccttaa cccaaccacg
      841 tgctagctct ccaaccgtca atctgttgat gcgcgaatca aagccaccat ccttctgtaa
      901 ataataccaa tgaccctctt cataaatcca accagttttt aaagtatgat aactacgctg
      961 atcttcaaaa taatagactc gtttctctgc ttggctatca tattcttccc catgatgttt
     1021 gttggtattc gtagcagttt ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac
     1081 accatcttga ccaaaataaa accaatctgg tctaagagca atctctattc ttggagaagg
     1141 accaatcgta acccccttgt gtggagcagg tatatattgc cagccgacaa ccatctctcc
     1201 ggataaagga tcaaaataat agtacttccc atctatcact ctccagtagg tttcttttag
     1261 atcccccttt ttgtagtagg ttctaccatt ttcttggaca aactgccatc cttcagaatc
     1321 atctgcaaat actgtatttg tcgctaacaa accgaagaaa aatgtggcta gtgcgatttg
     1381 catcattttt ttcaaaagct tcattttata tatcctccaa cattaaatcc actcaccaga
     1441 tgaagcgaag ttataaactt taccatcgat agtttggcta cctgtaacca ttgctccatt
     1501 tgaattgaga taataccatt ttccatttac tttttgccaa tctgtaagca tcttaccgga
     1561 actgtctaaa taataccaag tatcatttat ctcctgccaa cccgtttgtt tatcatgttg
     1621 tctataatgg taccaactac catttatttt cttccatcct tcaagagagt aacctttaag
     1681 aaccgaataa ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt
     1741 agctccatct cctaaagtat gcactcctac tacacggtga ctagcatcat aaactgtaga
     1801 tccactagac ccctctaaag tatcaacttg gtaatccaag aacatgccat catcacttaa
     1861 aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt
     1921 gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa
     1981 ttttgcacca atgggctctt ctagaattaa taaagccaag tcatattccc ttgcatcctt
     2041 agaatttaaa tttctaaatt ccttcaaata acgaacttcc tttactttga tctttccaaa
     2101 tggttcttga cttggactaa cagccggaag aacataaata tcatccgctt ctttcccata
     2161 gtcatgtctg tagtagttgt gagccgctgt aattaacaca ttatctttaa taaaattcgc
     2221 acttcccctc ccttctacat tcattccatc ggctactcca taatacttag ataaaaccat
     2281 agctgaagtc gaatacggat actgtaaagt attatcaatt ttaatcaaat catcattacc
     2341 gataatactt ctgattgctt ttggtagtat gaaccatacg ttggtgaaat ctcagataat
     2401 gaagaatcat tagactctgg acctttttct agtgtctcac ttacctcata ttcttcacct
     2461 gtactagaaa taacactcaa agcagatact gtcgataact ggctagccaa taaagtactc
     2521 gcaataattg aaatacccaa ttttttataa acagttttct tcattattgt atcctcctaa
     2581 tgtaattata gcgtactatt ctaaatttct taatctacta tagaatcaag aaatctacca
     2641 ccttctttaa ataccctcca ttatcacata aacaggtaaa cttttcaatt aatgactgcg
     2701 cttttcaatc acgctagagg tacttgcttg cttctttgat actaagttca gccattcttt
     2761 ccttgttttt ctcaataaag catgttaccc aagtgggatt cgttttggag tagtctcgca
     2821 gagtccagcc aatggcttta ttgataaaaa attctgtttg gttcaagtta tgaaggagaa
     2881 tcttttccat taattgagta ttggtcttct cttttcttaa caactggtgg tcaatagcga
     2941 cacgtctcag ccagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     3001 caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgc agatagagct
     3061 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     3121 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac aaggattttg
     3181 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     3241 tcaaatagtt agcagccaca tattggtatt ttctaggatc cttttcccag caagtgtctg
     3301 caaaatccca atcgataatc tttgtttttt tcgcttctgg aaaatatttt atagagttta
     3361 tttctttcag gcaccgcaat acctagaaaa gaaaattgat ggcgcatata ggcttccatg
     3421 gaccttgctt ttttagagtc ttttgctgct tctagctcct caagtaaatc tgctaaactc
     3481 atctaaaact cctcttgccc caccaaatgg tgctgaaagg catagacagc cgcctgggta
     3541 cgatcgctga cttcaagttt ggcaagaata ttggacacgt gggtcttgac cgtcttgaga
     3601 gagataaaga ggtcatctgc gatgcgctga ttttcgtagc ccttggcgat gagttggaga
     3661 acatctcgct cacgcgcagt caattcttca tgaagttcca tatgattgcg gtggtattca
     3721 accttcttgc taacctcttg ctcaatggcc agctcgccag cagctacctt actgacggca
     3781 tgaagcaatt catctgcact agaagtcttg agcatatagc ctttggcacc agcatctaag
     3841 actggcatga ttttttcatt gtccaaataa gaggtcacaa tcaaaatctt ggcttcaggc
     3901 cattctttaa ggattgctaa ggtcgcgtca atcccattca tctcaggcat gacaatatcc
     3961 atgacaatga catctggacg cagttccaag gccaagtcaa tcccttgaga cccgttggac
     4021 gcctcaccca caacttctac atcgtcttgg aggtcaaagt agcttttcaa gcccaatcgg
     4081 accatttcat ggtcatctac tagtaaaatt ttcatcttta ctcctttatc attccttatc
     4141 taacagggga atacggatat caaccgccag cccttgcttg ggagctgtca agagttgaac
     4201 tgttccagcc atatcttcaa cccgctcctt gatatttcgc agtccataac tcaagtcgtc
     4261 taagctccct aactggaaac caatcccatt gtccaccacc ttcagttgca attcaacatc
     4321 tgtctgatag aggtagacat ctaggcaaga tgcctgggca tggcggaggg tattgctaat
     4381 caactcttgc aggatacgga agatatgctc ctcgattttc ttaggcaatt tcgtcatatt
     4441 ctgcttgaga ctaaccctaa gatcactctt gtcctcaagc tcttttaaaa gaatttgaat
     4501 cccttctatc aagctcttct gctccagttc aactggtcgc aaatgcaaga gcaaaacccg
     4561 caaatccttc tgggctgttt ctaaaatagc tgtgacactc tgcaactggg tctgcatctt
     4621 ttctctatcc aatttcaaag cctgctgact gatacccgat aaaatcatgt gggccgcaaa
     4681 caactcctga ctgactgtat cgtgcaaatc ccgagcaatt cgcttccgtt ccttctcgat
     4741 gatttcctct tcctgagcaa ggctctgatt ttcagctttt tgaagagcct ctgtcaaaag
     4801 gttaagttta cctgataagg acttgaaact ggcatccaaa tctggatctg caacctgaac
     4861 cacttcttgc cctgctaata aacgcttgag attagcctgc atttttctta gagaaagctc
     4921 ttcgatccct cgccaaaaca gggctaagag acaggtcatg gacatgctga aaaccaacaa
     4981 taaaaagaca aatttttctg ttttttcgac atcgtgcaaa aagatagacc agtcaaaatc
     5041 aagtatttcc agcaagctgt gggagaaaaa aaagacaaat aggaaggagg tgagagcaat
     5101 aatgacatag gcttgttttt tcatcctcta accacctcca catcaccaat catagtggtc
     5161 aagaaaatct tgacactctt gttactcttg agatagtctt ttgtttcttg atgatagtgt
     5221 tcattgcgga gggctcgctt gggctggttg aaaaaaatca aatccccata gagacagtta
     5281 acgctgagac tgacttccac atctacaggt acgatgattt tggtcgttcc taccatcttt
     5341 ctgaggataa tgacattgtc atgattggtt aagatgaccc tctccagatg aatagtgtcc
     5401 ttgcccatga agcgaaagag attgatatca tcgaattggc aagtctggta gcttgaaaaa
     5461 tgatgaagat ttccaaacca acgatttttc tccttcttaa ccgtcacgac ctcttcaaaa
     5521 accaaattgg tctgctcttt ttcctggttc atcatcgggt aaagaagaaa gaggctatag
     5581 ataaccgcaa caaaaatagc tagaatcaca aaaggattga gcataacgat gaaaaagaag
     5641 agaatggttg ccgctactaa aagaagatta tttccctctt taccagtgta gtagcgaatc
     5701 aaaagcaaaa agaggaatag tatcagcaga aaacgcgaaa aatgctctga taccatcaaa
     5761 atcagagctc ctgtcagaag acaggcttcg ataaataaaa agattttaaa ttttctcata
     5821 ggttcatcct ctcccttcta ttttatcaca attcaaaaaa gtcacctcag tctgaggatg
     5881 gaaaaaaggc gctggttacg cctttttcat ctgatccttt gcttctttta attttccata
     5941 aagaagatag tctacttttt gtagatctgc tatggtggca cagttaaggg aacacataat
     6001 caagcgtaga tctgctttcc agccttggac aatgccaatc acttcttcaa ctgtgtaggt
     6061 ttcaaccaat tccagaacgg ttcgtgacaa tcccacagcc ttagcaccaa aaaccaagca
     6121 cttaatcata tccagcggat tccgaacccc tccactaacc aagagttcga ccttatcttt
     6181 ccattcttgg gcattgagaa gggcctgcat ggtagactga ccccattgat tgaggtaatc
     6241 acgctggcca ctacgacggt tttcgatata ggcaaagctg gtgccaccac gacccgatag
     6301 gtccactgta cgaacaccga attcataggc tctttcgatt gtcttggcat ccattccaaa
     6361 gcccacttcc ttgaggacaa taggaacggg aatttgcttg ctataatctg ctagatgcga
     6421 ttgccagctt ctaaacttcc tttctccctc gggcatgagt aattcctgca tgacattgac
     6481 atgcacttgc aatagaacag gattcatctc ttctacagtc tgaagtccta actcgacagg
     6541 cttgtccaat ccaatattgg ttccaaggag gagattggga tgactagact tgacagaaaa
     6601 agaatcatcc gttggatttt tgagggctgc gctataagaa cccgttacaa ataaaatacc
     6661 acaggattcc gccacctgag ccagcttttg attgatttct cttcccttat tacttccacc
     6721 agtcatggca ttgatataaa aaggaaagtc ccactttcga ccagcaaact ctgtcgaaag
     6781 atcgatttca tccagattgt aaagaggcaa ggaagaatga atcagctcca cctcatcaaa
     6841 gctattatag gaacttttct gctcaagggc atagaggata tgctcgtcct tacgatttgt
     6901 cgtcatgtcc tatcctttct tgatataaga gctcaatccc cagatcggcc caacgatttt
     6961 ttaaggtttt ggttgattgc gcatcaaaac tcagggcgat gccacagtca ccaccaccag
     7021 caccactact cttggcaacg gtctgcaaat cttgactggc ttctttcaac tgtctaagca
     7081 aaggcgtgta aatatctgta ctcaagcctt ctaaaagctt gctggctact tctacttgat
     7141 cgataatctt ttctgatttc ccctgttcca aggcttctac cagagaagtc accgtttctt
     7201 ttgaggaagt taaaaaattt tgattgatat tttgcttgat ttgctggacc atgtgactcg
     7261 atacagccac ttccttggtc catcccacta agaaatcaca ttctaaagtt ggtttcactt
     7321 gtgaaattga aaagccccaa tcacgctcca gaactgtcgc caagttttct tcttctaacc
     7381 aagcagccac cttctggcga tcaaatgact ggtagagaac caaatcctct gccacaatac
     7441 aggcaaggtc gcccatggaa ccattgtctc ctcgcttaag caagacagcg ctagtcagct
     7501 tgaacaagag ctcctgatca acagaaacat catacagagc cagtaaagcc ttgacaacca
     7561 agacaacgac gctgccacta gaacctagac caaacttttt cccttctcgt tccattttgc
     7621 cacagatttc tagagaaaaa ggtcttaaat tctgaccacg aacagcgagg aagtctccca
     7681 tcaaagcaat cgtttcttga atcaagctat agtcaggatt aggccttaag tccactgcga
     7741 aatcaaacat atctgaatag atacggtagc tgtcagaaaa agcaatctca gccctcatat
     7801 agatgggaat atcctttatc aaagctaact gccctggctc taaaatagca tattcacctg
     7861 cccaatagag ttttccgcaa gttttaacag caatcatctt gactcaaatc ctttgttttt
     7921 gacacaatca agcgataacg atgaccgaaa atttctgata aatgctccaa gtctttctcc
     7981 tgacagaaga ccttaacatt gggaccagca tccatggtaa agtagcaggc ctctcctttc
     8041 tcacgaagct ggcgaacaaa ggccatagcc tcataagagg catccgtcag ataagaaaag
     8101 gctggactag cagtctttgt cgtagcatgc atagccaggg catttttctc cgttaattct
     8161 ccaatcttgg caaaatcatt ttccttgaga taaatcagca tatcctgata gtccttctca
     8221 gactgacgaa cccagtcgtc gaaagtcgtc gaggtttcca cacaaagttt catcccgtca
     8281 cggctagaga ttggtttttt cttgtcctct agcaccaaca taatcatagc tagtttcaag
     8341 tctgtctcta cagggtaaat ttctccacta tccttatccc aggctcctag tggtccataa
     8401 aaactccgag aagaagaacc tgaggcaaat ttggcttcct gtgccaactg acttctatcc
     8461 aatccaagct tgaaataagc attacaagcc ttgaccaggg cggacaaacc actagaactt
     8521 gaggacagac ccgctgccgt aggcatattg ttttgagtat cgatacggac aaagccctca
     8581 ccagctggac gataacggtc aataatctta ctcatcttgg catgctcgac ctcattttgt
     8641 agctgaccat tgatgtaaaa ttcgtcagct gttacattgg ctggtaaagg cgacaaggtc
     8701 gtctctgtat acatattttc caaagttaga gaaatactgc tagtagcagg caccatctct
     8761 ttttcttttt tctttcccca atatttgata atagcaatat ttgcgtagga acgtactgtt
     8821 acaggctctc tatccatgtc tgaacagctc ctttctcttc taatctttct gctagttctt
     8881 gtgcgtgtgt caaattggtt accaaggcta tgatacaacc tcctagccca ccaccgctca
     8941 tcttggcacc cagagcacca tggctaagag tcgtttcaac caaaaagtct gcctcagggc
     9001 tactgactcc aatttctttt aaatgtaaat gcgcttgact gaggatttgt cccagtcctt
     9061 cagcatcttt ttgtgaaatc gcaacttctg cttgctgggt taattctccc aaggcatgca
     9121 aaaacggtag ggcatccttg cccttatttt gaaccacttg gatggcttca cgagtatgac
     9181 cataaacacc cgtatcggca atcaccaaat aggcggataa atccatctca agttctgtaa
     9241 atcctacgtt cttgataaag cgaataggtt ggtcactaag acaggtctta gcatccaaac
     9301 cactaggatt catatgggca atcatttcag ctcgattgac caagatttct agtacatcat
     9361 gaggcagatc agcctgatag tagtcaaata ctgcacgaat ggccgctatg ctgatagccg
     9421 ctgacgaacc catcccccgt ttctcaggga tagccgagtc aatctcacaa cgaatgcagg
     9481 cttctgtgat attcaaatac tccagtgagg cataaaccgc catggacaag gtatcctcct
     9541 cataaaggcg ccaaggactc tctgcaggaa ctaccttaca ggtcacctcc acctccaaaa
     9601 gaggcaggga aatggcagga taaccgtaaa cgaccgcatg ttcccctatt aaaattatct
     9661 tactatgtgc ctgaccgaca ccaacttttt ttgtcatttt ttccttttac tagacgaaaa
     9721 aacgtcttat ttttcataca agtattaatt ctttcctatc tattttatta tattttcaca
     9781 aaaaaagcga ttgtttccat tcacaatcgc ttctttcatt attgaaccca ttcgccatta
     9841 tagttgacag aatagccatc tacggtcgta ttcactgcca aggcacctga gcgctataag
     9901 cgtagtacca tctgccattg acctggaacc aacctgtcgt catagaacga cgaaagaaac
     9961 tccataccat taagtaaaga ggaaagtcgt gagggagcat gcgccattga caacctgttt
    10021 tagtgacgta caaagtctca ttaacaagta ctcgtttcgg ccatttatag gtgcggtgtt
    10081 tggagaaata gggttcaatc ttcgcccatt cttgatcgtt taaatcagta tcatatgctt
    10141 tgcgtatcat aactctagct taacattttt ttgtgaatac aggttctaaa taatcgacca
    10201 cgaaaatttc ttaagtggaa aacgccttat gaagtatgct acgggaaagt tatgcactta
    10261 atttgacaat tcaagatgta aaaatatata ctatagtaga ttgaaactag aatagtacac
    10321 ctctacttct aaaatattgt tagaaatcga tttgactgtc ctgatcgatt tatcctgtta
    10381 ttatctcatt ttactataat atttgataag ttatcctaaa agtattatta tgttgttgtg
    10441 ttatagattg attgaatcta actaaaggat cctattcaat tactagaact atcacatact
    10501 caaggtcagc tcacagatga gcaactattt tggttacaat gtctactaaa tttaagtcaa
    10561 acaaataatt tagtcaaaat taaaaaaata gaggaacata aatatgatta caaaacagaa
    10621 tgtaatagtg ttctacaatt tttactagat aaaactgtaa attctgaagg aaggatcact
    10681 tcttcaacag aatttggaaa tttcgtaagt aatttatcat tccaacacgg aatagctgga
    10741 ctactgtttc ctctaaataa attgtacccc ccagaactgg attctaaaat actctctatc
    10801 atcaagaagg cagtgacaat tagaacgaca cacacatatg aatatcaata ctcactgcta
    10861 tttggtgatg caggctatct atggttactc ctacatttat tttctatcag taaaaatcaa
    10921 tactatctac aattagcaaa cgtcaccgct aaaaaattaa tagagaatta tgatactcta
    10981 gaggaaatag actttgcatt gggaaaatct ggtgtcctat tatcattaat aaaatactat
    11041 caatttacca atgacaatac tcttaaaatt ttcatccaca atagtatagg ggaaatttat
    11101 cattatttcc tacaaagaga tacagccaaa gaaagcattt tagactatag ctttgctcat
    11161 ggatattgtg gaattgcata tgctttattt gcctattcta aagtcttaga accttctatg
    11221 ttttataatg atctccatac attccatact gaattaaaaa aattattaga aaaagttact
    11281 tctaatactg aaaatttagg aaatttacaa ctttcttggt gcaaaggaat ttccggaata
    11341 atcttatatc tttgtatgta cgattgtgac ggaaacaaag atattattag taaatatcaa
    11401 gaatttgttt ttaaccatca tctaaaaatg atgacaggat attgccacgg aataactagc
    11461 ttactacaaa ccactgtcta caatcaaaac aaattactga tgaaaaaaat ccaacaggta
    11521 attttagcat gttctgaacg agatgatcac ggtttactga tgtttcaagg agatagtggt
    11581 aaagcagatt tgtttgactt cggaatagga agcatggggt atattggtgt ctattaaata
    11641 ataaattccc attcgatgtg cagacataag gagaaaagta tgaaattatt ttggacaaac
    11701 aacatatata gacagttgct gctaaacagc tgtttttcat cattcggcga cagtattttc
    11761 tacctcgcca ttatcaatta tgtggctcag tacaatttcg ctccgctagc gattttactg
    11821 atttccattt cagagatggt tcccctacta tcgcaactct ttctcgggat tctaggagat
    11881 tttcaagaaa atagagtcaa acacgcactc tggattgcca aaatcaaaat cctgctctac
    11941 gctattttga cagtatttct cgtcttgtcg cccttttcat tagtttcagt cattatgatt
    12001 gtcatcatca acctcatctc tgacaccttg agctacctgt ctgcctacat gatgaacgcc
    12061 ctctacatca gtgtaattaa ggacgacctg catgatgcca tggggttcag gcagtctctg
    12121 atgagggttg tccgtattgt cgccaatctg gctggcgcat tccttatcaa tgttataagt
    12181 attcaaacta tttcccttat caacactctg acttttgtca ttgccttttt gggcctgtat
    12241 gttattcgac ataccttgta tgaggttgaa aaaagaattg aaatgtcaca tacagcactg
    12301 agttttaaga aatattttca acatcttaaa cagtcgctgg ctgtgctcct gaggttaaaa
    12361 gataccgtca tactactgtt tctgacgacc agtatgattg ccatcttgga tgtgtcccct
    12421 cggctgattg ccctccgctt catccaacag acactagcac aactgagcat tgggcaactc
    12481 ctcgccctgc tctccatcat catgtcttgt ggagctatcc ttggcaatat gaccagcagt
    12541 aatctattta aaaatatccg tttcacgcac ctcttggttt tctgtgagat ttccctattg
    12601 actctaataa ctagtatcct ttgtcaagcc tatatcgtaa ttttcatgac cagtttcatc
    12661 agttctacga ttatcggcat tctcagccct cgcctacaag cagctgtctt tgcccatatc
    12721 cccagtgaca agatggggac ggtgggctct gctctgagca cagtggacat tctcgccccg
    12781 tccctgctct ccctattagc cctatccata gcatcgggcg tttcggtgca gttagcattg
    12841 atatttttgt atcttatttt aattgctctt atcttttgtc aatggttagt caagttcaac
    12901 actcataact aacgaaaaag catgtgtaga tttcacatgc ttttaatctc cccaatcgtc
    12961 aggtcaagta caacaaagtc acttctttga ttaagcgagt gttctaatat aattataagc
    13021 gccctgtcat taccgaaccc attcgccatt atagttgaca gaatagccat ctacggtcgt
    13081 attcactgcc aaagcacctg agctataagc atagtaccag ttgccattga cctggaacca
    13141 acctgtcttc atgtctccat tacctgcatt taggtagtac caagttgaac catcttgata
    13201 ccaaccagtt gccatagctc ctgatgaacg gagatagtac catttgttcc caaggttttg
    13261 ccaacctgtt ttcatatcgc catttgggtg gtctaaataa taccaagtgg taccttcctg
    13321 ataccagcca gtggccattg ctcctgagga acggaggtag taccacttat tacctagata
    13381 ttgccaacct gtttgcataa taccagttgt tggatctagg tagtaccaag tcgaatcatc
    13441 gtttatccac cccgcacgtc tttcaccacc aaggtagttt tctccattaa tttccgtctt
    13501 agctagataa taccagttag actgatcata aagccaacct gtctctaaag aatgattttg
    13561 attaaagtaa tagttcgtat aataacgctt ctcttcttta tcttctgaat cttcacgttt
    13621 ttccccgtac tttcttccaa cactgtcttt agttttaatc tctaatgttt tccaaccaac
    13681 aaactcttgt agcactccat ttttatctaa gtagtaccac tctgactttg gaaaaccttc
    13741 taatctgata ccatttgggt aaggaccaat tgtactacct ttagatggaa acgggatata
    13801 ttgccagccg acaaccatct ctccagatag agaatcaaaa taatagtact taccatcaat
    13861 cactcgccag taggtttctt tgaggtcccc ctttttgtag taggttcttc cgttttcttg
    13921 gacaaactgc catccttcag aatcatctgc aaatactgta ctggtcccta gcaaaccaaa
    13981 gaaaaatact gtcagtccaa cttgcatagt ttttttcaaa attttcatct atataccctc
    14041 caatattaaa tccactcacc agatgaggcg aaattataaa ctttaccatc gatagtttgg
    14101 ctacctgtaa ccattgctcc atttgaattg agataatacc attttccatt tacttttgtc
    14161 caacctattt tcatatctcc attagaagga tctaaatagt accaagttga accatcttga
    14221 taccagccag ttaccatagc tcctgatgaa cggaggtagt accacttgtt accaaggtat
    14281 tgccatcctg ttttcatatc accattttta ttatctaagt aataccattt gcctcgtaca
    14341 tatttccaac ctgttgccat agaaccagag gagccaaaat aataccattt tccgtttact
    14401 ttttgccaat ctgtaagcat cttacctgaa gcatctaggt agtaccatgg agcaggtttg
    14461 cttctatcaa tgtcaatagt aacatgaaaa tcttgagtcc aacccttagc aacttcacca
    14521 attggtagtg gattgtaaga atcatcgcca aaatttccta gcttatttaa ataataccaa
    14581 tgaccctctt cataaatcca gcctgtcttt acagcataat tattatcaaa ataataagtt
    14641 ctcttttcag cagatagagg atattgttca ccgtatactt ttcctgtatt ttcagacgtt
    14701 tttgcctcta gtacttgttt atctgtttgt tctagcaaag caccatcttg tccaaaatag
    14761 taccacttct cctgaaggcc aatttcatta actggttggt tatcgaataa attatcacga
    14821 taacctgttc caggaatttc taagtattgc caaccgacaa ccatctctcc aatatcacca
    14881 aaatagtaag ttttaccgtc tattttatgc caatagattg ctttatgatc atcttttaca
    14941 taatattttc tattatcctt atcaacaaat cggccacctg tggtattcgc aaataccgta
    15001 tttgtagcta gcaaaccaaa gaaaaagact gctaatgcga cttgcatcat ttttttcaaa
    15061 agcttcattt tatatatcct cctaaaacga ttgtagtaaa aactagattg tatgtcaata
    15121 tataaaaact cctcaaaaaa gcagttacac aactgcaact gcttttctat tcttgcatgg
    15181 tatatccaac accacgcaca gttttaatgt agcttttttg gcctttaaca tcaagcttgc
    15241 tccgtagata gcggatatag acatctacga tatttgtctc ggtcgcactt tcatacttcc
    15301 aaacactttc caacaattgc tcacgagtca atactttctt gcttcccata agtgtcgcca
    15361 aaaggtcata ctcacggcgt gtcagagcaa tcatctcttc accacgataa accgtgtgat
    15421 gttcaacatc tatcctaaga ttgcggtagg tccttggaac tttcatcaga ctgcagtgtt
    15481 gatcaatgaa gtcccgacct cggaagatcg ccgaaatacg cgctaccaga ttttcgataa
    15541 ggactggctt atagatgtat gaaactgcaa aacgctgaac aacttccagc tcttcttgca
    15601 agtcttccca atgatctaaa atcatgatga ctgaggcagg tttagttcgg ctcaattttt
    15661 ctgcaaaatc ctgagccatc atatctccca gattaacgtt caataacatc aaatcatagt
    15721 ctgtctgaag agccatggag agggcttttt gcccctcctc taccagatca acccgatact
    15781 gctctttctg gagttccaaa cttaaaaaat gagctagatt tcgttctttc tcaagtaata
    15841 aaatccgttt ccccatggct gacctactta tttttcgtca taccaagagt agtggaaggt
    15901 tccttctttg tctttacgtt ggtaagtgtg agcaccaaag tagtcacgtt gtgcttggat
    15961 caagttagct ggaaggtcag ctgaacggta gctatcaaag taagtaatag ctgctgagaa
    16021 agttggcact ggcacacctg cttgaaccgc aagagctacg atatcacgta ctgcttgttg
    16081 gtacttagca gtaacatcca agaagtactc gtccaaaaga aggttggcaa gatctgcatc
    16141 gcggttgtaa gcatctgtaa tcttttgcaa gaaacgagaa cggatgatac agccatcacg
    16201 ccagatagat gcgatatctg caaatggcaa gttccagttg ttttctttag aggctacacg
    16261 caattgagca aatccttgtg cgtatgaaat gatttttgag aagtaaaggg cttgacggat
    16321 cttttcaatc aattcagcct tgtctccttc aaagttgaag gcagctggtt ttggaagcac
    16381 cttgctagca tgtacacgtt cttctttgta agttgaaatg tagcgtgcaa acactgactc
    16441 agtaatcagt gacaatggta caccaaggtc aagagatgat tggctagtcc atttaccagt
    16501 tcccttgtta cctgcagcat caaggatgta gtctacgatt ggtccatctt ggccttcatc
    16561 gtctttacgg ctcaagatat cagctgtgat ttcaatcaag tagctgtcta attcaccctt
    16621 gttccactca gtaaagattt cagccatatc ttctgcagaa aggcctagca agtgttgcat
    16681 caagtcatag ctttctgcga tcaattgcat atcaccgtac tcaataccat tgtgaaccat
    16741 tttcacatag tgaccagctc catcaggacc gatgtaagtc acacatggtt tgccatcttc
    16801 tggtgcttta gctgagattt cttcaagaac atccgcaacc aattcgtagg cttctttttg
    16861 tccaccaggc atgatagaag gaccttcaag ggcacctttt tcaccaccag aaaccccagt
    16921 accgataaag ttgataccag agtttgccaa ttcttcatta cgacggatgg tatctttgta
    16981 gaaagtattt cctccgtcaa tcaagatatc acccttgtca aggtgtggaa gaagggcttg
    17041 gatagtagca tctgtaccag gtccagcttg aaccatcagc atgatacgac gaggtttttc
    17101 gattgagttt acaaaacttt caacgtcata gcttggtaca aagttctttt caggatggca
    17161 agcaatcaca tcttccgttt tttctttact acggttgtag atagcaactg tgtaaccacg
    17221 agattcaata ttaagggcaa ggttacgacc cattacggcc atacctacga caccaaagtt
    17281 agcttttgtc atttgacact cctcttgttt catttgtttt attttatcat ttttactttt
    17341 gaaaagaaag aattttgtaa cgactgctta gtaatctaag tcatccgcat gaccagcgcc
    17401 atttccgaca aagtagcctg tcttacagtt aagggtaaag agataggttc catctggctt
    17461 gtagaggttg taataaccat tgccgttaac gatattgaca cgttcaagga tatattggtc
    17521 tccagtgata tagccacgtg aacgtgaagt cgctagaatt tgttccaaga caccatccgc
    17581 aaaatcccag gcagagttat tactatcatc aatagcagac tgattatagg caacacgact
    17641 ggcacttctt tgaacagcaa cccctgcact cgagagaccc atattgactt cactgcgact
    17701 acttctagtc tcatttgaac tacttggttt tggctcacta gtcgtatttg aacttgcttg
    17761 acttgaactt gagctgctag tttgacttga acttgagcta gaagtacttg tttgctggct
    17821 cttaccaaga ctgatagcct tatctagcac tttatcaagc tccgtatttc cagttttaat
    17881 atccgtaaat ttagcatccg atttggcttt ggcattggta tccaacacac catccacaat
    17941 agctggtttc tcaaattgag cattgacatc ttgaatagcc ttgatttgcg ttgctagact
    18001 atcatattta gatttggcaa gcgtatgttc acgactacct tccagcttat caagtaaagt
    18061 cttgagttga ctcagtttat caaactggct atttttcaaa gccgttttat tgctatctgt
    18121 gtaaaaggcg tcataaagtg tattaaaatc atccacatcc gattgattgg ctgtagttga
    18181 ttgagaagtt tcaatttcct tagtcgaacg agccacttga cggtagacat aataagcact
    18241 gacacaaata attactgata caagcgccaa aacggtcaaa ataaagcctt ttttactttt
    18301 cttttctgag tcctcttgat ccagacgaga aaggttttgc tcctcttcca cttcttcagt
    18361 catttctgtt ggattttcat catctagcaa tagaggatcc aagccttgct cctcatcgtc
    18421 cagaggcaag ggtagtaaga tgtcttcaga agatggaact tcctccgaag aagcttctga
    18481 cttctctaca gcctcacgca tctcttgaat taaatcatcc agactctgag tttcaacgaa
    18541 ctcctctttt ttgtattgac gagtcgcaaa cttatccgcc tcaatttcat ctctgtgttg
    18601 cttaacatac ttgtccaaaa tggaatcctc aggcaagact cctgattcca cttcttcatt
    18661 tttacgaata gcttgaccaa ctgttagctc ttttgcttca tcaaaatcaa attgcggttc
    18721 ttgaccttct tttttatgac gatttcgtct ttttttactc atgagtatcc ctttctattt
    18781 tacctcttgc cgtttgacac gctgaccaaa atattgatat agatccactt tcaaggttcc
    18841 gttgtataac ttacgcttct tatctgcttg gctaccatac ttgctttcaa aagcttcatc
    18901 actagtcagg ataaatttgc tccaagtttt cagcggtgca aatacttgcc ccatctcagc
    18961 atagagcttg gtcacccctg catcatctga caaacgttca ccataaggcg gattggaaat
    19021 gattactcca ttgattttat cggaacgtaa atcctgcacg cgcatctgct taaaagtaat
    19081 gtctcctgca acaccagcta cctgagcatt ggccttagca atttccacca tgcgagcatc
    19141 aatatcacag cccatgatat ccagctcaag ctcacggtct acttttttag ccgcttctgt
    19201 gcgcacttct tgaatcaagc gatcgctgat ccagttccat tcctcaaatg caaaagagcg
    19261 acgaagacct ggcgccatct ttctagcaat cataactgcc tcaatacaga aagtccccga
    19321 accacaggtc ggatcaatca aaggcttgtc tggataccag ttagaaagtt gtaaaatggc
    19381 tgctgccata ttttccttga taggagcgcc acctttttcg gtacgataac cacgtttaaa
    19441 gaggctagac ccggtcgtat caatcatgac agttgccaca tctttgagaa tagagacctc
    19501 aatcttaaac tctgggccat tctccatcag aggaacccct tctgggcgag catagtgttt
    19561 ctgcaatttc ttgacaacag ctttcttaga aatagcctga acactgggct cattgtgaag
    19621 tttggactta acacatttag cttttgaaat cgggaaccga gctccaagtg gtaaataatt
    19681 ttcccaatcc aaagcgaaaa ctccctgaaa tagctcttca aaagtcttag ctgggaacgt
    19741 tcctacgata attttgatac gatctgctgc ccgaagccaa aggttggttt cgataatagc
    19801 tctcacgtct ccttgaaaac gaacacgtcc attttcaacc tgacaatcgt agcccaactc
    19861 tcgcacttca cgaccaacga cagcctcaag ccctgctgcc acagttgcaa ttaaattaaa
    19921 ttcttttttc atgttacagt cttgcaagac ctttctatat gtccacttta aaaaatgagg
    19981 ttgagaaaat ttctcagccc caacctatat gcaattttct ataagccatg ttttgttcca
    20041 gaagtgacta ggaccacttc cttcgataat catctgtcta ccactaacag ctctagctga
    20101 tagcagtcag aatactacgt tcgtttccgc gtactccatg ccccgaccaa aatttgggtt
    20161 gctagcttga ggggtttacc gcgttccact ctctctgttt ccagaaagac tccgtcactg
    20221 tggcactttc aagcctactc tggcctatcc aaggacttag ccatttcaac tgccgtaacg
    20281 atttctcgtc cctaggctta tggtttcgcc tagcacaaac actacaggca tcacagcctg
    20341 tgctagcatg gactttcctc atgagaaaca attctcctca cgcgattatc caaaaattgc
    20401 acatctcaaa taactactta aaaatctgag ttatctaaaa tttgtttacc aaaaacttct
    20461 ttttccaatc tattcaggcg tttcaaaata tcaaaattcg tcatagaact tgtaattgcc
    20521 gcttcaaggg gttctgcttg aactggtgaa ggtttcggtt tacgagttaa ttcttccttc
    20581 aaatccgcaa tttcctgacg aagtgacttg accaaggcag cataggtttc atagtccttg
    20641 atgacatcgt ctaaaaactc gtcaacttct actttattat agccacggac ctcacgtcca
    20701 aactcttgtt caaaaatatc tttcgctgaa aaaataatac ttgccatgtc tctctccatt
    20761 ctcgcttgct agtattatta tataaaaaaa ggcaaacaaa aatcaaggtc aaagcttcaa
    20821 ttttcggaaa aattttcagc aagttcattt aattcatcaa atgttaatct ctttataaaa
    20881 tagtcctctt gatttttcat cttttggtaa aaataagcta gttttgtttc attttcttca
    20941 tcataaaaaa gataggaact agtcgtgttt tccagcaaaa actgctggta atctcttaac
    21001 tgccccttgt gttcatagcg aggataggca tatttgacaa agtctacctg cttaaagcga
    21061 ctcagtttca tctgattgcc ttcattccaa ttttccccat gggtttcaaa agcaaaaatg
    21121 gtggccaact ggaaaccgta ttcagttttc atctcctgag caacctctaa aacccaatat
    21181 tcaaaaccca aactccctgt aaacacaagc caagacaccc catctgctgc catagcctct
    21241 aaatctttac gtatcgcttt tttaatcaat ttaagacgag gatccttatc agaaaataaa
    21301 cccaaatcaa aagcagaata gcctaaaacc aaagctgtag ccatttttaa ccctttctgt
    21361 gcaaattatg gtataataaa gtgatgttat tgtaccaata aggagaatta tggtcaacta
    21421 tccacataaa gtttcatcac aaaaaagaca aacatctctt tctcaaccca aaaatttcgc
    21481 aaatcgagga atgtcttttg aaaagatgat caatgctacc aacgactact atttgtctca
    21541 gggcttggct gttatacata agaaaccaac tcctattcaa atcgtacaag tggactatcc
    21601 acaacgaagt cgtgccaaga ttgttgaagc ctattttcga caagcttcaa cgacggacta
    21661 ttctggcgtt tataatggat attacatcga ctttgaagtc aaggaaacaa aacaaaaacg
    21721 tgcgattccg atgaaaaatt ttcatccaca tcagattcag catatggaac aagtccttgc
    21781 ccaacaagga atctgctttg tccttcttca cttttcttct cagcaagaaa cctacttatt
    21841 gccggcattc gatttgattc gcttctatca tcaagataag ggacaaaaat caatgccact
    21901 tgaatatatt cgagaatatg gatatgaaat caaggctggt gccttccctc aaattcctta
    21961 tctcaatgtt atcaaagaac atttattagg tggtaaaaca agatgaacaa accaacgatt
    22021 ctgcgcctaa tcaagtatct gagcattagc ttcttaagct tggttatcgc agccattgtc
    22081 ttaggcggag gagttttttt ctactacgtt agcaaggctc ctagcctatc cgagagtaaa
    22141 ctagttgcaa caacttctag taaaatctac gacaataaaa atcaactcat tgctgacttg
    22201 ggttctgaac gccgcgtcaa tgcccaagct aatgatattc ccacagattt ggttaaggca
    22261 atcgtttcta tcgaagacca tcgcttcttc gaccacaggg ggattgatac catccgtatc
    22321 ctgggagctt tcttgcgcaa tctgcaaagc aattccctcc aaggtggatc aactctcacc
    22381 caacagttga ttaagttgac ttacttttca acttcgactt ccgaccagac tatttctcgt
    22441 aaggctcagg aagcttggtt agcgattcag ttagaacaaa aagcaaccaa gcaagaaatc
    22501 ttgacctact atataaataa ggtctacatg tctaatggga actatggaat gcagacagca
    22561 gctcaaaact actatggtaa agacctcaat aatttaagtt tacctcagtt agccttgctg
    22621 gctggaatgc ctcaggcacc aaaccaatat gacccctatt cacatccaga agcagcccaa
    22681 gaccgccgaa acttggtctt atctgaaatg aaaaatcaag gctacatctc tgctgaacag
    22741 tatgagaaag cagtcaatac accaattact gatggactac aaagtctcaa atcagcaagt
    22801 aattaccctg cttacatgga taattacctc aaggaagtca tcaatcaagt tgaagaagaa
    22861 acaggctata acctactcac aactgggatg gatgtctaca caaatgtaga ccaagaagct
    22921 caaaaacatc tgtgggatat ttacaataca gacgaatacg ttgcctatcc agacgatgaa
    22981 ttgcaagtcg cttctaccat tgttgatgtt tctaacggta aagtcattgc ccagctagga
    23041 gcacgccatc agtcaagtaa tgtttccttc ggaattaacc aagcagtaga aacaaaccgc
    23101 gactggggat caactatgaa accgatcaca gactatgctc ctgccttgga gtacggtgtc
    23161 tacgattcaa ctgctactat cgttcacgat gagccctata actaccctgg gacaaatact
    23221 cctgtttata actgggatag gggctacttt ggcaacatca ccttgcaata cgccctgcaa
    23281 caatcgcgaa acgtcccagc cgtggaaact ctaaacaagg tcggactcaa ccgcgccaag
    23341 actttcctaa atggtctagg aatcgactac ccaagtattc actactcaaa tgccatttca
    23401 agtaacacaa ccgaatcaga caaaaaatat ggagcaagta gtgaaaagat ggctgctgct
    23461 tacgctgcct ttgcaaatgg tggaacttac tataaaccaa tgtatatcca taaagtcgtc
    23521 tttagtgatg ggagtgaaaa agagttctct aatgtcggaa ctcgtgccat gaaggaaacg
    23581 acagcctata tgatgaccga catgatgaaa acagtcttga cttatggaac tggacgaaat
    23641 gcctatcttg cttggctccc tcaggctggt aaaacaggaa cctctaacta tacagacgag
    23701 gaaattgaaa accacatcaa gacctctcaa tttgtagcac ctgatgaact atttgctggc
    23761 tatacgcgta aatattcaat ggctgtatgg acaggctatt ctaaccgtct gacaccactt
    23821 gtaggcaatg gccttacggt cgctgccaaa gtttaccgct ctatgatgac ctacctgtct
    23881 gaaggaagca atccagaaga ttggaatata ccagaggggc tctacagaaa tggagaattc
    23941 gtatttaaaa atggtgctcg ttctacgtgg aactcacctg ctccacaaca acccccatca
    24001 actgaaagtt caagctcatc atcagatagt tcaacttcac agtctagctc aaccactcca
    24061 agcacaaata atagtacgac taccaatcct aacaataata cgcaacaatc aaatacaacc
    24121 cctgatcaac aaaatcagaa tcctcaacca gcacaaccat aacatttatc acccagattt
    24181 ttctgggtga ttttgtcata gcactattgt atttatgtgc ttaacaaaag cctgagaaga
    24241 catcttctca ggctttttct gtactataaa cctcattgga aaagatagag actgcttccg
    24301 taatcttgtt aagaggtttt actaaatact agtctttctt cgtttttact acaaagagag
    24361 cagatagggc tagactaaca cttgctagga tgagggctgt gtcagattga ctctcacctg
    24421 ttgctggcaa gatttgttca ggtgtttctg gtctagctat ttcttgctta taatctggcg
    24481 tcttaccttg ctcggaagct aaagatctaa gtttaaactc aggtttctct acagttggag
    24541 catctggttt cgtagcagaa tcttttaatt ggttgagata atctgcaaag tattccacca
    24601 ttctttccgt caagagatgg ttatccgtcg catatttcat gcttgcctga acgctagcta
    24661 cttcctcttg attgtctttg tctgttaatt tctgagcctt aaccaaagct gcttcatagc
    24721 ctgacaagtc aagaggtgtt tctgctacct gtggacgagt aaagataagt tccgctgcag
    24781 attggtattt atctccacca tctccgtatg tcttggtacc agtaaggaca attttcttag
    24841 ccttgattgt cttaccaaag tcaatatctt ttggtttgtt gttatttggc caatcagttg
    24901 cagtaaaggt atgctccttg ccagactcat ctgtcacaac aagtttcaca tctcgcaagt
    24961 taccatttga acctgatcca cgcggaacat agcgaagtcc tgtgatttca gttggttctt
    25021 tcaagaccat agttgcaggc ttgcctacat ctccaccatt ccaagatgta tgccatagac
    25081 tagacacatt gccatcaaag gcatttgcaa gaccttcttg agcctgagca ggagctgtaa
    25141 gacttgcaaa gtcatctgct accaaagccg tcttcttctg aaccaaagca ttcttcaaag
    25201 cttcaatctt ggcaatctct gcacgcgctt cttccacact gatatcatca tcggcctgac
    25261 tgaggttaaa gaccgcctct ttcaaagcat ccatagactc tttggtgtag ttagtcatgg
    25321 caaccgttgg caagtagttc ttcagagcat tttctgtcaa catcttacct gttagggtaa
    25381 tttcttcgat ttgaagatta tccatcatga agtcgttata accacggaag ttggcatttc
    25441 caccagaatc accacgagta ttacttgcat ttccagttga gtagatacct acccaagtat
    25501 cgcctgtttc tgcacctgtc acaaggaagg ttgccttctt ggctttctta gaatctgtcc
    25561 aagtatttgg caattcatgc atttccaagt tgcttgcttg agtaccacga cgacctgact
    25621 ggaattctcc cttaccgact acaaaagcat aggtattgtc tgatcctgct tcgtattcaa
    25681 aggttacacg gtaggtctta cctgcttcaa aacggaagtt ttgtgggatg gtttggtaaa
    25741 ccaagttacg acggctcact agtccatttg tcttgagtga ccaatttcct tcgataacat
    25801 catcgacttt cttaccattc caaccacgtt gtgtatatgg attgtgtttt tcagacaagt
    25861 gagtgcggtt atcttcaaca ccttcgacac cacccactac aaatgggaag ataccctgag
    25921 caacattttc aaagtcttgc ttgaaggtgc ctttacctgt atcatgcttg tctccgtaca
    25981 tgcttgaatt gttttcaaag gtacgaattt catcaaagta agttgcttga tcaccagctt
    26041 cacgactcaa tgtcagagta acatttgaga cgtccgctcc agttgtaaag aaggcgtaca
    26101 tgttttggaa gtaacttgta tcgtcaactg tagcattgtc acgacgtgta ttgtgggcgt
    26161 aggccttaac atagttgagc gcgagagact tattggtata agtagtcact tctttttcac
    26221 cagtattcac agtgatactt gccttggcat tactacggtt atctacacca acataaacgg
    26281 catacttggt atttggtttc aagccagtta atttctgagt gagactaact ttttctttgt
    26341 ttccttgaat acgaagcata tcgtttgccc cttgagactt gacaatttct gccttagaag
    26401 catcgcctga aatggtccaa tgtttcaagg taccgctatt aaatccttgg tcatagatgt
    26461 gcatgccttc actccatgac atttcaggat tagtttgttt cgaacgatag agaacgtatg
    26521 gttgatttgc tagaagatct agggtaattt taccatcttt tacagttagt tcttgctctt
    26581 ctgtcttacc ttggtcagtt agcttgtaaa ggtaaacctt gctctttgcc caatcgcttg
    26641 gaagggtcca agttgttgca ccggcctgcg tattgaagta gtacatcttt tccttatcag
    26701 tagaaagttt cttaccattt gcatcccagt tccaaggagt caagtaagct gaaccatctt
    26761 ggatgacacg tccgttgagc gttactgtac gttcgcgata ttgtggacta ttgacatcat
    26821 ttgacttacg agttacaact actttattat tgtcagcatc taccaattcc actcgcattt
    26881 ctggagtcca tttataggtg ctaccgttat cggtcatagt caccggtgta ccattttccc
    26941 atttacttac agtgaagtgt tggaagtact tagtcatgac gtcatgggca aataagttgg
    27001 ttacatagcc attgtagtca cttcttccct gccagccttc aaagtctttc atgctgtagc
    27061 cacctagcag tggatagttg gctgcaccac cataacttct gtagtcccct acccaagcat
    27121 ctttttggtg gttacggata aagcgggtga tggcactgtt gataccttta ttggtgtagc
    27181 caccgtaggt caagtcagct gcccagtgat ggaaggtaga gtcgtactca ccaccatggc
    27241 cccactcgat cgcaaagcgc cagccttgtt tgttaatttc tttagcaaga acgtgggtag
    27301 cccaggcacc gttatcacct gattgaccat taccccaaac gtccacatag ataaagtcga
    27361 gaccgtcacc aagttttttc ttcaaatctt cccaacgtgc caaacgacca tgagctaggt
    27421 cataggcagc atcaatgttg ataccttgat ctagccagtt ccaaccatag ctatagcttc
    27481 catctggatt cttacggaga attttttcat tgaagtattt agactcagga taagtttctg
    27541 aagcgttaac gtggatacct agatgagctc catatttctt agccttctca attagggtct
    27601 tgaagtcttc gacaccaccg atacgcttac caatatcagc atagttcaag tgaccagagt
    27661 catggccttc gctaccatat cctttaagga gaacaccttg cccaagacca tctgtatgga
    27721 gattgatttt cttgatacca tccaaggtca taaggaatgg gttttgtgct tgagaaccaa
    27781 agttcatcgc gatacggtaa gctgtgatat ccttaacttt ttcccaacct tgaggattgt
    27841 tcataatgct acgataagca atggcaccat cttgccaatc aacgttcttg tctgcattgg
    27901 catcttcagt gataacaacc ttagcacttg gaagttcctt cgtgtattct gggaaaacaa
    27961 tgcccttata agctttttcc cattgccatt cagagctgtg gattcctaca tagttggcat
    28021 ttccgactgt ttctttataa gctgtcaaac gagtccagtc attcgaacca ccaccatagc
    28081 tgttttgaga gttactccaa acaccagcag caagcttatc tgtagaaaca aatccataca
    28141 tgtaaccctt agccaaatcc ttcattggat tggttacatc gatatgatca tctccgctga
    28201 catgcgtatt gtttgacatg gttgccccat caaacttagc accagtttga ttactagaaa
    28261 cagagactaa agcattgccg aggaaactaa tagaagaaag tagtttgctt tcgtcatcaa
    28321 tcttttgacc tggagtgact tgattgtggt tgacaatctt agtcacatca aagtgcaatt
    28381 gattgtctac aacttgcaag cgtactgtca tttccgcatt gattaagtga gcatcatcgc
    28441 gaagcttcat caagtactct gctgttgtct cattgatttt cttataagtg acttcagggg
    28501 tgattcggtg gttattgata aagacttggt tgaactgttg cacctgtcct ggcaaagtat
    28561 gcccgttcaa gctgtattcc ttgacacgag ggaaggcttg gtcaatcact gctttgagga
    28621 ccttagactg aatcgtgtca taagtcacct tgctatcatc aacttcagga cctgtttctt
    28681 tttcagcagg ggtatcctct gtttttaccc cctcttggtt atccgtttta acgctaacaa
    28741 ctgttcgctc atcgtcataa gagcccgcct tgagaagaat cttcttctca tttttaagat
    28801 ggtcattgac cgcagctggt agagtcactg tgtcaaagag attgacatca ttattgctgg
    28861 catttagctg accgtctgac ttgagagtga tagagagacg gtttgttgat cctgtttcag
    28921 gagcagcaac acgactacct ctataccaag tgctagttgt tggagattta tactcccaga
    28981 accagccatc cttgtcataa ccgacaaaaa cattattctt ggtatcttta aatttcaaaa
    29041 agacaccaaa gcgtgatttg cccttttcag aatcatcttt gaaggttaaa tcaacagttg
    29101 catttccatt ggcatcaacg gtcaagccct tcttttcaaa cagggctggt ttgcctgcgt
    29161 tatcattttg agcagttgag gatagttggt tgtagcggac acctttttct tctcggatag
    29221 tgactgttcc ctgttgctct ttattcgcta ccgtttgcca ttcaggggtt accgtcttag
    29281 gtgtttcagg tttagcagct gcaggtttat cctcttttgg tttttcttca gccggttttt
    29341 ctttttcaag tgctaatagt tcttcttctg tcttaggtcc atctgtaact tctggagaac
    29401 cttggtcttc tcccacctta ggcgcttcaa aatcacgccc atcattctct tttgctgttg
    29461 caagagcagt agctaaatca gctggtaagt tcgccgtgga accagactgc acgctatctg
    29521 caagaaccgg acttgtccca aagaatgcag ctccaatcat aacagaagca acacctaatg
    29581 aaaatttccg aatactatat ttacaacgtt tttcaaataa tcctttattc ataaacttct
    29641 aaatcctttc atttttgagc aaacgtttac attttctttt taaaaaatac tccttcctct
    29701 gaaagtgcaa tgcttaactc tgataaataa aaaataatta tacttttaat ataacaaata
    29761 atagctgaga aagcaatagg aaacaagtaa aattaacaaa aattttctct attctgacaa
    29821 aaagaggctc caacttttgg gggtccgtac attttagaat ctttctttac aaatgtaaaa
    29881 aaggattcag cattcaaggg aaatactaaa tcctttctta tattttgcaa cagttatttc
    29941 acatgttttg cgagatcttc ttgagccttt ttattagact cttctttttc tttcatccat
    30001 ttttcctgag ctttttggta ttcatctaca gtgactgcct tgtcttgaag ttccaagtat
    30061 ttatacaaga ctggttcact tgtaccttta tttcctgaca atgcaaatgg tattgtaaat
    30121 ggtaccatct tagacaagat tggacgccct gtacgagatg tagttggaat aatcaaagca
    30181 ctatctgtca accaagcttg ggctgcagcg tatttatcat agcgtttagc aacatctgta
    30241 gtctcatcac cagcctcagt aaccaatttt tcgtagtcat atagacctac ttttttagca
    30301 gctacattat cttcccctga gtcaaaccct aaatatgttt tagtactttc tcctacagaa
    30361 ggtttgataa tatcaaggta ggttgatgga tcggcaaagt ctggacccca accgacatta
    30421 tctgataaat cccagtcttc gccagcagca ttttcagcaa aatatgtaat attgtttact
    30481 tcgtcttttt gtagttgttg aatatcaata atgacattat cagctcctaa agttgcttcc
    30541 aaggattgtt tcatagattg gacgcgctga acttttgtag ttgctgtttg gtcaactggc
    30601 atatccaaat gaattgggaa ttggactcct tctgcttgta aggctgattt agctttagca
    30661 aattcagcct tggctttttc tggattgtaa agaccatcct gagaatctgc aagattaaca
    30721 tccttccatt catccccata agtgaccaat ttctctttga ccatatcgcc aaagttttta
    30781 ccatctgctt gaacaaatgt tggtggcaca aagagattac gcaagatttt acttgctcca
    30841 gtttgtccat tcaactgaga ggcataggct gtacggtcaa atccaaaggc aatagcctga
    30901 cggaaatcct tgtttaagag agccttttta gtcgatgcct tttgttcgtc gctggtctta
    30961 gatgtgtatt tataggactg acggtcaata tttgtaccaa ctagatacgt aatagagtct
    31021 tgttgagtat agacaatatt gtccttcata ctcttctcaa gttctgcgaa acttgcactt
    31081 gttggataga gacgagctgc tgtaaggcta ccatctttaa agttttctgc aggtttgctg
    31141 gtatcttgac catcccagaa tgacaattta actttgtcaa catgcacatt gtccttatcc
    31201 cagtagttcg gatttttcgc aaattcaaca gaggatttgg tcacaatgga tttcaacaaa
    31261 taaggaccgt tatacaagag actacttgga tccgtagctt tggcaaaatc atctcctttt
    31321 gaattcaaaa actcttcatt aactggcgca agcacaccca tggttgtctt agaattccag
    31381 aagctttctg gtttgttcaa agtgtactga actgtctgtt catccagagc cttaattcct
    31441 acttgtgaga aatctttgat ttcccctttt acataggcat ccaacccttt gattgattct
    31501 tgaacaaggt aaagagcatc tgatttttta tcagcagcat attttaatcc tgttacaaag
    31561 tcttgagctt tgactgccgc gtattcttca ccttcagaag tataccattt tgcatcctta
    31621 cggatagtat aagtgtaagt caatccatcc ttggatacag accaatcctc agccatagac
    31681 ggcacaaagt tcccgtagcg atcattttct agcaaaccat caaccacgtt actggtaata
    31741 tttgctgtcg cagccttagc agttgtcaaa tagttgaggt tatcagggtc tgtctcataa
    31801 atgtatgaga atgtcttctc acctttagtg cttgaacctg atccagagca tgcagctaaa
    31861 gtagtcgccg ccaataatgt cacgcccgca agggcaaata gttttgaact tttcatcatt
    31921 ttctccttta aaactttctc tccattatag actcttttca gaaagttgtc aacagaattt
    31981 tcagaatttt tgaaaattat ttttcaaaca acatctttgc aaaaaatatg aatatcgtaa
    32041 gcgcgtcata acaaggtatc tatcattcat ggagctcctc ctgtatacta ttagtaaagt
    32101 aaatattgga ggatatttta atgccacaac ctattgttcc tgtagagatt ccacaatctc
    32161 gtcgttttga ttctaaaaag agaaatgata ttctacttaa aattcgtatt ggcaagcttg
    32221 aagtaagttt ttttcaatct ctcaatctcg aaatgataga acagcttttg gataaggtgt
    32281 tgctctatga caattcatct atctagccta gggcaggtct atctcgtgtg tgggaaaact
    32341 gatatgagac aaggaatcga ttcactggct tatctcgtta aaacccactt tgaattggat
    32401 cctttctccg gtcaaatctt tctcttttgt ggtggacgta aagaccgctt taaagtcctt
    32461 tactgggatg gtcaaggatt ttggctacta tataaacgct ttgagaacgg cagactgact
    32521 tggcccagta cagaaaagga tgtcaaagct ctcgcacctg aacaagtaga ttggctgatg
    32581 aaaggctttt ctatcactcc aaaaatatag tagattgaaa ctagaatagt acacctctgc
    32641 ttctaaaaca ttgttagaaa tcgattttac tgtcctgatc gatttgtcct gttattattt
    32701 cattttacta taaatccatc agaaagtcgt gatttctatt gaaatgagga ctttcttttt
    32761 atactcatct gctttcaaaa agcactctag tccatctccg attaacgatg gactttatca
    32821 cctccttctc cagtccttgt ataacatctt gaagttgatt catgacatct tccaaagttc
//
LOCUS       DBDESG010000020        32246 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.020, whole genome shotgun sequence.
ACCESSION   DBDESG010000020 DBDESG010000000
VERSION     DBDESG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.020"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..969
                     /gene="phtB"
                     /locus_tag="ACKB38_000905"
     CDS             <1..969
                     /gene="phtB"
                     /locus_tag="ACKB38_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HGL7979695.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKG
                     AEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAP
                     KGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPSEEKPQ
                     TEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQVETEKVEEKLREAEDLLGK
                     IQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
     gene            1317..1391
                     /locus_tag="ACKB38_000906"
                     /pseudo
     CDS             1317..1391
                     /locus_tag="ACKB38_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
     gene            1391..1765
                     /gene="dhaM"
                     /locus_tag="ACKB38_000907"
     CDS             1391..1765
                     /gene="dhaM"
                     /locus_tag="ACKB38_000907"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGL7979696.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            2225..2938
                     /locus_tag="ACKB38_000908"
     CDS             2225..2938
                     /locus_tag="ACKB38_000908"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGL7979697.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2996..3598
                     /locus_tag="ACKB38_000909"
     CDS             2996..3598
                     /locus_tag="ACKB38_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979698.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3736..4389
                     /locus_tag="ACKB38_000910"
     CDS             3736..4389
                     /locus_tag="ACKB38_000910"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGL7979699.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            4399..4863
                     /locus_tag="ACKB38_000911"
     CDS             4399..4863
                     /locus_tag="ACKB38_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGL7979700.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4876..6144
                     /locus_tag="ACKB38_000912"
     CDS             4876..6144
                     /locus_tag="ACKB38_000912"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGL7979701.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            6158..6418
                     /locus_tag="ACKB38_000913"
     CDS             6158..6418
                     /locus_tag="ACKB38_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979702.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            6429..7790
                     /gene="pdrM"
                     /locus_tag="ACKB38_000914"
     CDS             6429..7790
                     /gene="pdrM"
                     /locus_tag="ACKB38_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGL7979703.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            7944..8912
                     /locus_tag="ACKB38_000915"
     CDS             7944..8912
                     /locus_tag="ACKB38_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGL7979704.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8928..9920
                     /locus_tag="ACKB38_000916"
     CDS             8928..9920
                     /locus_tag="ACKB38_000916"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGL7979705.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            10138..11181
                     /locus_tag="ACKB38_000917"
     CDS             10138..11181
                     /locus_tag="ACKB38_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGL7979706.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            11227..12930
                     /gene="lpdA"
                     /locus_tag="ACKB38_000918"
     CDS             11227..12930
                     /gene="lpdA"
                     /locus_tag="ACKB38_000918"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGL7979707.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVNKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            12994..13983
                     /locus_tag="ACKB38_000919"
     CDS             12994..13983
                     /locus_tag="ACKB38_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGL7979708.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(14048..15118)
                     /gene="xerS"
                     /locus_tag="ACKB38_000920"
     CDS             complement(14048..15118)
                     /gene="xerS"
                     /locus_tag="ACKB38_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGL7979709.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(15702..17252)
                     /locus_tag="ACKB38_000921"
     CDS             complement(15702..17252)
                     /locus_tag="ACKB38_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGL7979710.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(17268..18047)
                     /locus_tag="ACKB38_000922"
     CDS             complement(17268..18047)
                     /locus_tag="ACKB38_000922"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGL7979711.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(18034..18885)
                     /gene="ylqF"
                     /locus_tag="ACKB38_000923"
     CDS             complement(18034..18885)
                     /gene="ylqF"
                     /locus_tag="ACKB38_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGL7979712.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(19104..19280)
                     /locus_tag="ACKB38_000924"
     CDS             complement(19104..19280)
                     /locus_tag="ACKB38_000924"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGL7979713.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(19332..25346)
                     /gene="iga"
                     /locus_tag="ACKB38_000925"
     CDS             complement(19332..25346)
                     /gene="iga"
                     /locus_tag="ACKB38_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HGL7979714.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAAMGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETMETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNKPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(25555..26733)
                     /locus_tag="ACKB38_000926"
     CDS             complement(25555..26733)
                     /locus_tag="ACKB38_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979715.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNC
                     VCGNAIKYAYELFNGVTSQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSNFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(26948..30598)
                     /gene="addA"
                     /locus_tag="ACKB38_000927"
     CDS             complement(26948..30598)
                     /gene="addA"
                     /locus_tag="ACKB38_000927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGL7979716.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSAQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLILE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGRLIILKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAAFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(30595..>32246)
                     /gene="rexB"
                     /locus_tag="ACKB38_000928"
     CDS             complement(30595..>32246)
                     /gene="rexB"
                     /locus_tag="ACKB38_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGL7979717.1"
                     /translation="ATVDTVLVQSYDLIAPLTADFVYAIGLTQDNLPKISQNTSLLTD
                     EERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSARKQLFLSAPSLFNESESKES
                     AYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSLVAYHQQGEMSDTEQDLTFVK
                     VLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALYPAKQEFYLSTSGLTEFYRNE
                     YSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQLPNEDSFDQRLEQAIQETSQE
                     REFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIETIKEEANFGGKDQAFIQLDN
                     GRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPHFFNGLNSQLPTYLAALKREG
                     EQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKYQGLFLEKESSYLGEFYNKNK
                     ANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYTENGRSIAPYVQQHQAITGFE
                     ANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREELNR"
BASE COUNT         9197 a         6276 c         6638 g        10135 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag gtttgacccc tccttcgaca gaccatcagg attcaggaaa tactgaggca
      301 aaaggagcag aagctatcta caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt
      361 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
      421 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      481 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      541 gaacgtccgc attcagataa tggttttggt aacgctagcg accatgttca aagaaacaaa
      601 aatggtcaag ctgataccaa tcaaacggaa aaaccaagcg aggagaaacc tcagacagaa
      661 aaacctgagg aagaaacccc tcgagaagag aaaccgcaaa gcgagaaacc agagtctcca
      721 aaaccaacag aggaaccaga agaatcacca gaggaatcag aagaacctca ggtcgagact
      781 gaaaaggttg aagaaaaact gagagaggct gaagatttac ttggaaaaat ccaggatcca
      841 attatcaagt ccaatgccaa agagactctc acaggattaa aaaataattt actatttggc
      901 acccaggaca acaatactat tatggcagaa gctgaaaaac tattggcttt attaaaggag
      961 agtaagtaaa ggtagcagca ttttctaact cctaaaaaca ggataggaga acgggaaaac
     1021 gaaaaatgag agcagaatgt gagttctagt tctcattttt ttcatgaaaa tgtgcaaaat
     1081 atagtagatt gaaactagaa tagtatacct ctacttctaa aacattgtta gaaatcgatt
     1141 tgactgtcct gttcttattt cattttacta tatcttaaca gatagtgtaa ataaagataa
     1201 actatttact ggctaattaa tcagttaaac actagttaag gagtaatgat gaaaaaaaga
     1261 acaatactat tattgatggc cagtctgtta gctcttgtct taggagcatg tggtttcttg
     1321 gacatattga tcctggatca ttctcatcag gattactctt tactgctatt ttagaaactg
     1381 gggtggtttg atggaaagta ttggtcttgt tatcgtttca cattccaaac acattgcaga
     1441 aggtgttgtt gaactgatta gtaaagtagc taaagatgtt ccgattactt atgtaagagg
     1501 aaccgagggc ggaggaattg gaacgagttt tgaacaagta gatagggttg tttccgaaaa
     1561 tccagcagat actttacttg ccttttttga cctaggttct gctaaaatga acttaaaaat
     1621 ggtgactgat ttcagtgata aaagtatcat catcaacagg gttccaattg tagaaggtgc
     1681 ctataatgca gctgctcttc ttcaggctgg tgcagaactg tcagttattc aaacacagtt
     1741 agcggagctt gaaatcaata aataaggaat tttactataa ctctttttat agataagcta
     1801 ttgattatct caactataaa atgttaagta aatgatttca aaatcagaaa gggattgctt
     1861 tatgcagttc ctttttattt taacaggagt gaaactatag tgtttctaaa ttgtgaatca
     1921 atcaaaactg attgtgatgg ggctattcta gctttagaaa ccttcaaaaa ttaaaattta
     1981 aggcaatcaa ttacttggaa gagtatgaaa gcatttagtt tataggaatt ctaggtctag
     2041 aattacatat atatatttat gaagacgggg tgttcgatag ttagtattgt tctattctga
     2101 aagatttgag ctgtcagttg tatagaaagt gttcgaattt ttttaagtga ttaaattagt
     2161 taattgtatg aggtgcttta tgatataatg ttcttaatga attttcagaa aggaaaacct
     2221 caaattgttc tacaaatttc tactcttcga cctcgaccac actcttcttg attttgatgc
     2281 tgctgaggat gtggctttga cccaacttct aaaagaagaa ggagttgcgg atattcaggc
     2341 ttataaagat tattacgttc ctatgaacaa ggctctctgg aaagacttgg agctgaagaa
     2401 aatcagtaaa caagagctgg ttaacacgcg cttttctcgt ttatttgctc attttggaca
     2461 ggaaaaagac ggtagttttc ttgcccagcg ttaccaattt tacctcgccc agcagggaca
     2521 aacactatcg ggcgctcatg atctcttgga cagcctcatt gagcgtgatt ataacttgta
     2581 tgctgcgaca aatggcatta ctgccattca gacaggacgt ttggctcaat ctggtctagc
     2641 accttatttc aatcaagtct ttatctcaga acagttgcaa actcaaaagc cggatgctct
     2701 tttttatgaa aagattggcc agcaaattgc tggatttagt aaagaaaaga cgctgatgat
     2761 tggagattct ctaaccgccg acattcaagg tggcaataat gcggggattg acactatctg
     2821 gtataatcct catcacctcg aaaatcacac acaagcccag ccgacttacg aagtctattc
     2881 ttaccaagac ttgctggatt gtttagataa aaatattctt gaaaagatca cattttaaag
     2941 gagacgagct aatgactaca aaaaagctaa tattactatt gaagagtaca ttgaaatgtc
     3001 tgaagttgat tttaatgaag ctgttaatta tgaatttaca tctgacactt gtcaattagc
     3061 aaatagtatt tatcaatctc tttttaagtt ttttgataag aaaaatttct ctggcgattt
     3121 aatttttact tggaaatctc catcattagt caaagaaggg gattatattg ggagaaggga
     3181 ttcacaagta gataatctta gagtaatagg aaatatattt ccgaattatc ttactaatcg
     3241 aaaatatagc ctcaatatga atcgtaatgg ctgtatggga gattttcctc atgacttttt
     3301 tgatatatac ctagatcatg tagcaaaata tgcctacgaa caaaaagtta ataatattaa
     3361 agagtattat cctttaaaaa gagcgatttt acaccaagag aatgcattgt attttcgatt
     3421 tttttctaat tttgacgact ttttagaaaa aaattattta aagactatat ggcaagtttc
     3481 taaagaaact cctttttctg agatggattt taatatgttt aaaaatattt cagaaaagat
     3541 aatcttcgaa agaggtagta aaatgcttaa tgatttaaaa agtaattata aaaaatagcc
     3601 agtctttata aactgtattc acgtattcaa atcgtatata tagtcgattt attaaatctt
     3661 tagataaact gtaaaaagtg gtttccatag ccactttttg ctataataga ggcagtaaaa
     3721 atgaaggagt cggctatgga acactcgtct tggcatgctt tgattaaggc gcaattacct
     3781 gagggttatt tcgggaaaat caatcagttt atggagcagg tctattctca ggggattatt
     3841 tatccaccca aggaaaaggt ttttcaggct ctcttgacaa cactgcttga agaagttaag
     3901 gtggtaattc tagggcaaga cccctatcac ggaccaggtc aagcgcaggg cttgagtttt
     3961 tctgtacctg actctatccc agctccacca tccttgcaaa atatcttgaa agaattgtca
     4021 gatgatatcg gagttaagaa atctcatgat ttgacagctt gggctgagca aggagtcttg
     4081 cttcttaatg cttgtttgac tgttcctgct ggacaggcca atggtcatgc tggtcaaatc
     4141 tgggagcctt ttactgatgc tgtgattcag gtggtcaatc atctagatag accagtcgtt
     4201 tttgtactct ggggagctta tgcacgtaag aagaaggcat tagttaccaa tcctcatcac
     4261 ttgattatcg aatcagccca tccaagtcct ttgtcggttt atagaggatt ttggggttcc
     4321 aagccctttt ccaaggccaa tgcattctta aaagagacag gacaagagcc aatcgattgg
     4381 cttagataag gagaaaatat gcctcagtta gcgacgattt gctacattga taatgggaaa
     4441 gaactgctca tgctccatcg taataagaaa cccaatgatg tccatgaagg gaaatggatt
     4501 ggtgtgggtg gtaagctaga gagaggagag acgccccagg aatgcgcggc gcgtgaaatc
     4561 cttgaagaaa cagggctcaa agccaagcca gttctaaaag gtgtcatcac ttttcctgaa
     4621 tttacaccag atttagactg gtacacctat gtttttaagg tgacggagtt tgagggcgac
     4681 ttgattgact gcaatgaggg gacgctagaa tgggttccct atgatgaggt tttgagcaag
     4741 ccgacttggg aaggtgacca cacctttgtt gagtggcttt tagaggataa acccttcttt
     4801 tcagccaagt ttgtttatga tggggataaa ttgttggata cccaagttga tttctatgaa
     4861 taaaggagaa agcagatgct actaatcaaa aatggtcgtg taatggatcc caagtctggt
     4921 ttggatcaag tttgtgatgt cttagttcaa gatgggaaaa ttatcaaaat tgcgtctgag
     4981 atcacggaag aaggagcaga aacgattgat gctactggtc ttgtggttgc tcctggcttg
     5041 gtcgatattc atgttcattt ccgtgaacct ggtcaaacac ataaagaaga cattcatact
     5101 ggtgccctag cagccgctgc aggtggtttt actactgtcg tcatgatggc taataccagt
     5161 ccaaccattt cagacgtgga gactttgcaa gcagttctcc agtcagctgc caaagagaag
     5221 attaatgtca agacagttgc gaccattact aaaaacttta atggtaaaaa cttgactgac
     5281 tttaaggcac tcttagaagc tggtgcggtt ggtttctctg atgacggtat tccgcttgag
     5341 agcagtaaga ttgtcaagga agccatggag gaagccaaaa aactcaatac ctttatcagc
     5401 cttcatgagg aagatccagg tttgaacggt gttcttggct ttaatgaaaa tattgctaga
     5461 gaacatttcc atatctgcgg tgctactggg gtggctgagt acgctatgat ggcgcgtgat
     5521 gtcatgattg cctatgcaac taaagcccat gttcacatcc agcatttgtc taaggaagaa
     5581 agtgttaaag tagtggagtt tgctcagggg ttaggtgcag aagtcacagc agaagtagcg
     5641 ccacagcatt tctctaagac agaagcactt cttttaacac aaggtagcaa tgctaagatg
     5701 aatccaccgc ttcgtttgga atcagaccgt cgtgccgtta tcgaaggtct caaatcaggt
     5761 gtcatcacag ttattgcgac tgaccacgcg cctcatcatg tagatgaaaa aaatgttgag
     5821 gatattacca aagcgccatc tggtatgact ggcttagaaa catccctgtc tctcagcttg
     5881 acctatttag tagaagctgg tgagttgagc ttgatggaat tacttgaaaa aatgacatac
     5941 aacccagcca agctttacaa ctttgaagca ggttacttgg ctgagaatgg tccagcagat
     6001 atcactattt ttgatgccaa ggctgaccgc tttgtggact cccattttgc ttccaaagca
     6061 gctaattcac cattcatcgg tgaaacctta aaagggcagg ttaaatatac catctgtaag
     6121 ggacaaatcg tctatcaagc ttgataggag ttagaatatg aattattttc gaaaacgtag
     6181 ggagagacaa gcgaaatcta atagtggaat ttactgtccc gcagctaatc gttccaaaat
     6241 tcaatatgaa actaaagaaa aagctgacca tgcggttcaa tatgataacg gaggtttagt
     6301 tagaagctat tattgtcgta catgtgcgtg ttggcatact acttcaaaat ctaataaacc
     6361 tccgctaagt ttgaaaaact atggtctgaa actctttggt ttagataaat cagaatagaa
     6421 agagaagtat gtataagaca aagtgtttac gagagaagtt agtattattt ttaaaaattt
     6481 tcttcccaat cctgatctac caatttgcca attattctgc ctcttttgtt gatactgcaa
     6541 tgacaggtca atacaacact atggacttgg ctggtgtatc tatggcaacc agtatctgga
     6601 atcctttctt tacatttcta acagggattg tgtcagcctt ggtgcctatc attggtcacc
     6661 atcttggtcg aggcaaaaag gaagaagttg cgtctgattt ttaccaattt atttatttgg
     6721 ccttgggcct atctgtggtc ttgctgggga tggtactttt cttggcacca ataatcttga
     6781 atcatattgg gttagaagca gcagtagcgg cagtagcggt tcgctatctt tggtttttat
     6841 ctatcgggat tatccccttg ttgctcttta gcgtcattcg ttccttgctg gattcgctgg
     6901 gcttgaccaa actgtccatg tacctcatgc ttttgttact ccctctcaat agcggattta
     6961 actatctctt gatttacggt gcctttggtg ttccagaact gggaggggct ggtgctggtt
     7021 taggaacatc cttggcctac tgggtcttgc ttgggatttc tgttctggtt ttatttaaac
     7081 aggagaagct caaagcctta caccttgaga aacgaattcc acttaatatg gataaaatta
     7141 aggaaggagt tcgtttaggt ctgcctattg ggggaactgt cttcgcggaa gtggctatct
     7201 tttcagtggt tggcttgatt atggctaagt tttcgccctt gattatagct agtcaccagt
     7261 cagctatgaa cttttcaagt cttatgtacg cctttcctat gagtatctca tcggctatgg
     7321 ctattgtcgt ttcctatgaa gtgggagcca agcgatttga tgatgcgaaa acctatattg
     7381 gtctaggaag atggactgcc ctcatttttg cggccttcac cttaaccttc ctttacattt
     7441 ttaggggaaa tgtggccagt ctttatggta acgacccaaa atttatcgat ttgacagtgc
     7501 gttttttaac ttatagtctt ttcttccagt tagcagatac ctttgcggcg ccgcttcagg
     7561 gaattttgcg ggggtataag gatacagtta ttccttttta ccttggtttg cttggttatt
     7621 ggggcgtagc aatccctgtg gctacgctat ttgattccct aacagatttt ggagcttatt
     7681 cttactggat cggcttgatt attagtttga ttgtgagcgg ggcgctctac cgttggcgtt
     7741 taactgtgat tatgaagaga tttgaatctt tagcaaaatc caaatgctaa aaagtttgtg
     7801 aaaaaatatt cttgacaaga aaatcccttg ttttcacggg gttttctttt ttattttgtg
     7861 aaatttcttt tagaaaaact ttgacagtat ttgccaaaag cggtaaaata gtaaggtaag
     7921 aagaaagtta gaaaggcaag aagatgtcaa ctttagataa aaatcttttg ctagaaatgt
     7981 tccgtaagat ggaagaaatc cgtcgcatgg acttaaaaat tgcacaatta gtaaagaaag
     8041 ggaaagtgcc aggaatgacg cacttttctg ttggtgaaga ggcagctaac gtgggggcta
     8101 tgttagctct caatccagat gatctgatta cctcaaacca ccgtggacac gggcaagcta
     8161 ttgctaaagg gattgacctc aacggaatga tggctgaaat ccttggtaaa tacactggaa
     8221 cctgtaaagg gaaaggtgga tctatgcata tcgctgacct tgatgctggg aaccttggtg
     8281 ccaatggtat cgtaggtggt ggtatgggga tcgctgtcgg tgcagccctc agtcagcaaa
     8341 tgcaaaatac cggtaaaatc gttgtctgct tctttggaga tggtgcgacc aatgaaggtg
     8401 ttttccacga agcagtgaac atggcttcta tctggaacct gccagtcatt ttctattgca
     8461 ttaacaacgg ttacggtatc tctgcggata tcaagaaaat gaccaatata gaacatatcc
     8521 atcaacgtag cgccgcttat ggaattcctg gaatgttcat cgaagacggt aacaatgtca
     8581 tcgatgtcta tgaaggattt cagaaagctg tggaccatgt tcgcagtggc aatggtccag
     8641 tcttgattga aagtgtaact tatcgctggc tcggtcactc atcatctgac cctggtaaat
     8701 atcgtacgcg tgaagaagtg gaattgtgga aacaaaaaga tccaatcgaa aacctccgca
     8761 attaccttat tgaaaataac attgcaagtg ccgaagaatt ggaagaaatc caagcgcaag
     8821 taaaggaagc agtagaagct tctgttaaat ttgcagagga aagcccattc ccaccgcttg
     8881 aatcagcctt tgaagatatt tacgcagact aaggagaaag agataaaatg gaaacaaaaa
     8941 caatgtcctt ccgtgacacc attatccttg ctatgtctga ggaaatgcgt cgcgatgaaa
     9001 atgtgttctt gatgggagaa gacgtcggtg tcttcggagg agacttcggt acttctgttg
     9061 gaatgcttga agaatttggt ccagaacgtg tccgtgactg tccgatttct gaagctgcca
     9121 tctctggagc agcagcagga gcagccatga caggacttcg tccaatcgtc gatatgacct
     9181 tcatggactt ctctgttatt gccatggaca atatcgtcaa ccaagctgct aaaacacgtt
     9241 acatgtttgg tggtaaaggt caggttccaa tgactgttcg ttgtgcagct ggtaacggag
     9301 ttggctctgc cgcccagcac tcacaatctc tagagtcttg gtttactcac attccaggac
     9361 ttaaggttgt ggcacctgga acacctgcgg acatgaaagg cctgcttaag tcttctatcc
     9421 gtgataacaa cccagttatt atacttgagt acaagtcaga atttaaccaa aaaggggaag
     9481 tgccagttga tccagactac acaattccac ttggggttgg tgaaatcaaa cgccaaggaa
     9541 cggatgtaac agttgttact tatggaaaaa tgcttcgccg tgtggttcaa gctgctgaag
     9601 aattagcaga agaaggaatt tcagttgaaa ttgttgaccc acgtaccctt gttccgcttg
     9661 ataaggatat catcataaac tcagtgaaga agactggtaa ggttgttctg gtcaacgatg
     9721 cccacaaaac aagtggctat atcggtgaaa tttcagctat tatttcagaa tcagaagcat
     9781 ttgactatct agatgcacca attcgccgtt gtgcaggaga agatgtgccg atgccttatg
     9841 cacaaaacct agaaaatgca atgattccaa cagttgaaag tatcaaagat gcaatccgaa
     9901 aaacttataa caaagaatag ggttacataa gataagttat gtaaataaaa taaagctgac
     9961 gagaaacttt gtatctttta ggtgcagagc tcttcgtcat taatatctta gattagagca
    10021 cgggctaaaa gatcggaaaa aagataaatc tccttggctt catcgaagcc atcgtcgatt
    10081 tcctattttt cagtcgcttt tgacgccctt tgtatcataa atagaattgg agaggtcatg
    10141 gctgatgaca agctaagagc gactcctgca gctagaaagt tagcggatga tttagggata
    10201 aacctctacg acgtttctgg ctcaggtgca aacggtcgtg tccacaaaga agacgtggaa
    10261 acttataaag acacaaacgt ggttcgcatt tcgccacttg caaaacgaat tgccctcgaa
    10321 cataacattg cttggcagga aatccaagga accggtcatc gtggtaaaat catgaagaag
    10381 gatgttttgg ccctgcttcc tgaaaatatc gaaaacgata gcatcaaatc tcctgctcag
    10441 attgaaaaag tggaagaagt ccctgataac gtaacaccat atggtaaaat cgagcgtatt
    10501 ccaatgacac caatgcgtaa ggttattgcc caacgcatgg ttgaatctta cttgactgcg
    10561 ccaaccttca ccctcaacta tgaagttgat atgactgaaa tgttggctct tcgtaagaag
    10621 gttcttgagc caatcatgga agcaactggg aagaagacta ctgtaacaga ccttctttcg
    10681 cttgcagttg ttaagactct tatgaaacac ccatacatca acgcttcatt gacagaagat
    10741 ggcaagacta ttatcactca caactatgtc aatcttgcca tggcagttgg gatggataat
    10801 ggattgatga cacctgttgt ttacaatgct gagaagatga gtctttcaga actggttgta
    10861 gcctttaagg atgttattgg ccgtaccttg gatggtaaat tggctccaag tgagttacaa
    10921 aattcaacat tcacaatcag taatttggga atgtttggtg ttcagtcctt tggtccgatt
    10981 attaaccaac ccaactcagc tatccttggt gtcagttcga caatcgagaa gccagttgtc
    11041 gtcaatggtg aaattgtgat tcgcccaatc atgagtttag gattaaccat tgaccaccgt
    11101 gtcgtagatg gtatggctgg tgctaagttt atgaaggact tgaaagagtt gattgaaacg
    11161 ccaatctcaa tgttaattta agagcaagag tattcatttt taagatatag ataaaggaag
    11221 gaagacatgg ccttagaagt aattatgcca aaagccggcg tggatatgac agaaggacaa
    11281 atcgtccaat ggaataaaaa agtcggagaa tttgtaaaag aaggagaaat ccttttggaa
    11341 atcatgactg ataaagtcag catggaattg gaagccgaag aagatgggta cttgattgcc
    11401 attctcaaag gagatggtga aactgtccct gtaacggaag ttatcggtta ccttggtgaa
    11461 gaaagggaaa acatcccaac agctggagca gcatcgccag aagctagccc tgtacctgta
    11521 gcaagtacct caaacgatga tggtaagagc gatgatgctt ttgatatcgt tgtgattggt
    11581 ggaggtcctg ctggttatgt tgcagccatt aaagctgccc aattcggcgg taaggttgcc
    11641 cttgttgaga aatctgaact tggtggaacc tgcttaaacc gtggatgtat tccaaccaag
    11701 acctaccttc ataacgctga aattattgaa aatatcggtc atgctgcaaa tcgtggtatc
    11761 gtgattgaaa atcctaactt cactgttgat atggaaaaac ttttagaaac taaatctaaa
    11821 gttgttaata ctcttgttgg tggtgttgca ggacttcttc gtagctacgg agttactgtt
    11881 cataaaggaa ttggtacaat cactaaagac aagaacgtct tggtaaatgg ttctgaattg
    11941 cttgaaacca agaaaatcat tcttgctggt ggttcaaaag tcaacaagat caacgtccct
    12001 ggtatggaat ctccacttgt catgactagt gatgacattc ttgaaatgaa cgaagtgcca
    12061 gaaagccttg ttatcatcgg tggtggagtt gtcggtatcg aactcggtca ggccttcatg
    12121 acatttggtt caaaagtgac tgttatcgaa atgatggacc gtatagttcc agctatggat
    12181 gtggaagttt ctaagaacct tcgcttgatt cttgagcgta aaggaatgac catcttgact
    12241 ggtactaaac tgcaagaaat cattgaggaa aatggtcaac ttcgtatcaa ggttgaagga
    12301 aaagacgata tcatcgcaag caaagctctt ctttcaattg gtcgtatgcc agaccttgaa
    12361 ggtattggag aggttgagtt tgaattggat cgtggttgta tcaaggtcaa cgaatacatg
    12421 gaaacttcag ttccaggcat ttatgcacca ggtgacatca acggtactaa gatgttggct
    12481 cacgcagctt tccgcatggg tgaagtttcc gctgaaaatg cccttaaagg aaatcatgca
    12541 gttgccaaat tgaatttgac tcctgcagcc atctacactc tccctgaagt agcagcagta
    12601 ggtttgacag aagaacaagc ccgtgagaaa tacgatgttg ccatcggtaa gtttaacttt
    12661 gctgctaacg gtcgtgctat tgcatctgac gcagctcaag gtttcgtaaa agttatcgct
    12721 gataagaaat acggagaaat ccttggtgta cacattattg gtcctgcagc cgcagaatta
    12781 ataaacgaag catcaagcat catcgaaatg gaaatcactg ttgaggaaat gctgaagacc
    12841 atccacgggc acccaactta ctctgaagtg atgtacgaag cgtttgcaga tgttctagga
    12901 atggccatcc attcacctaa gaaaaaataa aacgaagata gattagactg gaaggttatt
    12961 tccagtttct atcatataaa gtaaagggat atcatgaaat acattatcaa tcattcaaat
    13021 gacactgctt ttaatattgc cttggaagaa tacgccttta aacacctttt ggatgaggat
    13081 caaatcttcc tactttggat taacaagcca tctatcattg ttggtcgtca ccagaacact
    13141 atcgaagaaa tcaaccgtga ttatgttcgc gaaaatggta ttgaggtagt tcgccgtatt
    13201 agtggtggtg gagctgttta ccacgattta aataacctca actacacgat catctccaaa
    13261 gaagatgaaa ataaggcatt tgacttcaag agcttctcaa ctccggttat caataccctg
    13321 gctcaacttg gggttaaagc tgagttcaca ggtcgtaatg accttgagat tgatggtaag
    13381 aagttctgtg gcaatgccca agcctatatc aacggccgta tcatgcacca cggttgcttg
    13441 ctctttgacg ttgatttgtc agtcctcgca aatgccctta aggtttcaaa agataaattt
    13501 gaatcaaaag gtgtgaaatc cgtccgtgcc cgtgtaacca atattatcaa tgaattacca
    13561 aaaaaaatca cagtcgaaaa attccgtgat ttactcttgg aatacatgaa aaaagagtac
    13621 ccagagatga ctgaatacgt cttttcagaa gaagaattgg ccgaaatcaa tcgcatcaag
    13681 gatactaagt ttggaacttg ggactggaac tatggtaaat cacctgaatt taacgtccgt
    13741 cgtggaatca aattcactag tggtaaggtt gaagtctttg ctaacgttac tgaatcaaaa
    13801 atccaagaca tcaagattta tggtgacttc tttggtattg aagacgttgc tgcagtagaa
    13861 gatgtccttc gtggggtgaa atacgaacgc gaagatgttc ttaaggcgct aaaaaccatt
    13921 gatatcacac gctacttcgc tggtattagc cgtgaagaaa tcgctgaagc ggtagttgga
    13981 taaatcaaaa agaagttggc caacttcttt ttttgatatt tatttacata atttaaaata
    14041 cgtaaaatca taaactatcc agagcattct tttgttcatc actaacaata tgggtataga
    14101 ggtcagtgac ttgtgtgcta gcatgtccta gttggtgact gactaaaact tgtgatttag
    14161 tcgcatcata gagcctagtt gctagtgtat ggcgcagttt atggggtgtt acacgcactt
    14221 taaaatcctc tgagtattta gcaaccattt tctcaacgct agaagcatcg atacgattag
    14281 gaacacctct gtagagagtt aaaaaaaggg ctgtatctgt tttttccgtt ttatagcgtt
    14341 gattccgaat ggccagataa ttctctaaat aaggtttagc aaaagcagcg acattgactg
    14401 agtcacgttt gcaacctttt cgagtaacat caataaccat catttttaga ttgagatctc
    14461 ttagatctag attaacagct tcagataagc gaacaccaga tgccaagaga agggcaataa
    14521 tggctaaatc tcgttcttta tttttgttga atgatgagag agctcgattt gaaagctgtt
    14581 gtgggtgctc ttgatcgata taagttagaa aaccttctgt ttcatcacct agaaagagtt
    14641 tttgcttgat attttcagct ctggcagcaa gggtttcttt ctttttcttg gtggaaactt
    14701 ttttcattac attacgatag aaataaggtt ccccctgatc gttttcaacc tcctcggtta
    14761 gatacttgta aagactagaa agtgctgata aggttcgatt gatagttgtc tgtgaaacac
    14821 cttgttttgt tgtattagca ttcagcaagg gacgttcacg tagataaagg ataaaggatt
    14881 ccatgtcttt cttagacata ttttccaaaa ctgataaagg aatatcggat attttatcag
    14941 cgtttgaaat accagactcc aaaacccagc tgaaaaatcg gtcatattcc ttaaggtatt
    15001 cgtacagggt tgtaaaactg tagggcacag ccagcttaga ttggtagtat tccagaacat
    15061 accagggcat gagttgtttt agtttgtcga ttcgttccag taaaatctca cgtttcatgt
    15121 attttctcca taagtaagta tactagtagt atagcataga cttgtatatt tttcaagaaa
    15181 attatagttt ttcggaaaga tgttatagga gtttttcaaa gtgataaaaa gacttagacc
    15241 tacaaacaaa acagaataag gaaaataata aaaatctcac ccactaaact tctgttaata
    15301 ctacataact gaagagctat ataaggagct atgagataca aaacacattg tataatagat
    15361 tgaaactaga atagtacatc tggacttcta aaacattgct agaaattgat ttgattgtcc
    15421 tgattgtttt atcctatttc tatttcattt ctctatataa ttgctattgt tttctttttc
    15481 atgattttaa tcttactgta cagatttgat acagtaaact cctttctagc tttatacttt
    15541 atcatttcta acttaattat agtcttattt ttaataaagt tcaattattt atatgtaaaa
    15601 ttcctcatca gtaaaaaata accgatctca ttcctgagaa tcggttttct aaataaatca
    15661 aactgatcat ttacataaca taaattatgt aaatactcat actacaacaa caaatctttg
    15721 acttttccaa tttcactttt tggaataacc aggtgaatca tatcacccag atacattctg
    15781 gttgagccgt taactgtttg gctcttgcca ttatggactt gagttgtgat gaggacgttg
    15841 tgtggtaagt tgagttcatg aacttgtttc ccagcaattt tatcagaaac tggtatttcg
    15901 ataagtgtaa cttctccttc gctagatact tcttctggaa gcattttttc cagcatggct
    15961 tcatagactg gcgtaccttt gagcaaatcc atgataatat aagaaacaag agtgacaaga
    16021 ccaagtggca taaggttgcg aatatctcct accatctcag ttacgaggat catagcggtt
    16081 aagggagctt ttgatatggc tccaaaatag ccactcattc ctagaatgac aaatataggg
    16141 aattgctctt gactgacaag tccaagattg acacagataa caccaactaa ggcaccaagc
    16201 aaagaaccaa gagctaaaat ggggaggaaa attcctcctg gcagtccact tccatagcta
    16261 atcatactcc aaataaagcg gattaaaaag taagctaata aaacttggaa actaaaattt
    16321 tgttcagtta aagaaaggac aagctgattt ccgccaccaa tgatttgagg taagaagatt
    16381 ccgactggta tgataaggat aaaagccaag atgggataat aagccctatc caaatggatt
    16441 ttttgaccaa tcaagtcata aactcttcca acgtttaata cagctttctc atagagaaaa
    16501 cctgaaaatc caaggaaaat tcccatgacg agatatatcc aatactgatc tagggtcatg
    16561 ggaggaatgt tatctggcat atccaatact ggtgtcaaac cgaacatgag tagagacaca
    16621 aagtttgcta cgatgctggc tgctagagtt gagacccaga aaaagcgcga aaaatggtga
    16681 tagacttctt ctacaacaaa gagaagtgct gcaataggag cattaaaggc tgcggctaaa
    16741 cctgctgcag ctccactggc aatcaaggaa cgttcctcta ctggactgga tttgagccac
    16801 ttggcaattc ctttaccacc aactgctcca agttgaatgc tgggtccctc tcgacccagc
    16861 atgagtccac tggcaatagc aagaatacct agcacatatt ttttccaaag aatgccccac
    16921 cagttgaggg acatgaggcc tttcagttcg gcttcgactt gaggaattcc tgagccttta
    16981 atatcttttt ctgaccgtgt tagtttggca ctgagccaac agatgagtat ataaaacaaa
    17041 accagtacaa aaagattgcg cactaagtac ccttgatctt gataaactcc ttgtatcagg
    17101 tggaagccct tttcaattaa gaaacgaaag gatccgacga taattccaac gatgaggccg
    17161 acaatgattc ctcgaccaac ttgggatagt atagtgctgg atgcaaaggc gaattctttc
    17221 ttagaacgga ctatttccga ctgttcctcc atgttatttc ctttcaatta actttctttt
    17281 ttacctaaaa ccagtgattt aacgggttca aagctggttc ggtgaattgg ggtaactcct
    17341 agttttgtga ggccttccag atgtttagct gtgccatatc ctgcattagt agcgaaatca
    17401 tagccaggga actgctgatc gtattctttc agcaattcat cacgtgttac cttggctact
    17461 atagatgctg ctgcgataga gagggagttg gcatctcctt tgataatgga ggtttgtgaa
    17521 atgggcaagt ccagtttcat ggcatcaatc aaaaggtgct ctggttgagg gctgagctgg
    17581 gagattgctt cttgcatggc tagtttggtt gcttcataga tgttgacttg gtcgatgacc
    17641 tgattatcta tgataccaat tccaatcgac aaggcttggt cttgaacggc ttggaaaatc
    17701 tccagatgtt tctttttagg aattttcttg ctgtcgttga gacctttaat cttacaattt
    17761 ttagataaaa taacggccgc agcgactaca ggaccagcaa gaggaccacg accaacctca
    17821 tcaatacctg caattaaggt caatccttgc ttataaagtt ctttttcata agaaagcatg
    17881 gattccaagc gcaaattttc atctaattca gcttgaatgg ctcttttacg cttgctgatt
    17941 tccttttgaa ctcctgagcg attatccttt tcaagctcta aaaaaatagg gctttctaac
    18001 tccttgactg tcacaaggaa ttctttgatt tctttaatcg ttgccatcga ggtcttccaa
    18061 tgtatctaag gtatagttac cgagtttgcc atcacggact tccttcacga agagactgta
    18121 aaaacggtca tagtcatcac ggaaaccgag ggcgcgggtc atatccataa taatcacagg
    18181 cgcttcttct tcaattttca tttgtttgaa gcgttcagcc agcttttctg gataatgttc
    18241 tttgaaataa ttgataccaa aaatggtaac ctcatccata ggaagcaact ggtctttgat
    18301 agctccagtc aatgccaact taagtgcaac agtttcatcc tcaaacttag gccagagaat
    18361 ccccggtgta tccaagattt ccaggtcttt attggtttta agccattgtt gaccttttgt
    18421 gacccctggc ttgtttccaa caacagcaat ctttttacca gccaaacggt tcatcagagt
    18481 tgatttacca gcgtttggaa tcccgataat catagtacgc aaggtttcaa tctgaatccc
    18541 acgttctttc tggcgagcaa tcttatcagc catgagcttc ttggccgcat ctgttacaac
    18601 ttttacagtc acttgctctt tggagttgat agctagcgtc tggattcctt gtgattcaaa
    18661 atactgacgc cattccttgg tcattgctgg atcagccaag tcggccttgt ttaaaatcaa
    18721 gagttttggt ttatcaccaa caatcttggt caacatagga ttttgactag atagaggcaa
    18781 gcgtgcatct actaaaatcg tcacaaaatc aacaaatttt aaattctcct gcacctgtcg
    18841 acgagcttta gacatgtgac caggaaacca ttgaatagta gccattgagt tttttccttt
    18901 catagcaagg atttagatcc cctattttat tttactattg tttgaacata gagagttttc
    18961 aaatgaatga tgtccaaaca gttcttgttt tatgacatag aaatctactt tatattatat
    19021 catgccttct ttattttgtc cgatgatagc cttttaccct atcttttacc ctatatagaa
    19081 aaaagccatc aaatgatgac ttttcagagg gagcaaaaaa ctgttcccgt catattatga
    19141 accaaaagaa ctaaactacc aacagctaat aggcctaaag ctgtaagctc agaagttgat
    19201 gaacctgttg caggaagagt ttcctgctga taaataagat cataacttac ttcattatca
    19261 gtaggaattc ctgcttgaat cttggtttgt tcttcatcac ctttttaaac tttatttcct
    19321 aatagtagac actatttttt attctcaaaa attgaacttc taaaatcatt tgtttggtca
    19381 agataggctt taaagattgc tctcttgagc ttgtggactt cactatctat gtctggattg
    19441 tagttattcc aatggtagta attgtgttct gcatccttac gaacagcaac gtccattaat
    19501 ttctgtaatt catctacact gcttgtagtt ttcttggcaa atgtttgcca aggctgtgtt
    19561 gtatcattaa aagtaacttt gttcaatcta tcaaactgat cttgtcgttc ttgatacata
    19621 gctttcttaa actcactcca agtatgatat tggttattaa ataccttttc caaaaccaag
    19681 tcatctgtaa ccaaacctct tgttttaccg tagagattga ttgtcttgcc cttttgtttg
    19741 gcttcttctt cgtattggtt tgagatatat ggtaccatac catctttaaa gcctttagca
    19801 gctagtagtt cataggctat acgacgtccc atcaggtcac ctggtgttcc tatatcatta
    19861 cttaatgcag cataaatcgg tgcaaataac ttaatagtga agtagccatt tctttcgtat
    19921 ttacctgagg catattccct agacgaaaga atattattat caatcaaatc attgaatgaa
    19981 cgtagtcttt caacttcttc tactgttaga tttctgacaa catttgttgc atagacctta
    20041 tttccatcag gatcaggatg gtactcattt gtaacttttc taagaagttg ttgtttttga
    20101 ttcgtatcca atttaagaat tgaatttcct tcgagatatt ccaacatata aacaacgtca
    20161 aacatgttgt ggacatattg cttcaaatca tctgcattat taaatcttgt agttggatca
    20221 agtacttgta atcgtcgact ttctgtacta tcagattttg aatgtttcaa gatggagttg
    20281 atggtaatgg tcgcatcatc tggatggtct ggtgcttgta ataatccttt agcaaagaac
    20341 tctggtccca agccacttct tcgaccatat cctccaagat aaatgtcctg atctgagtca
    20401 tgtgtcatct catgcgtata agtaatagct ccatccttat ccaacattcg ataacccata
    20461 taataaactg catcacctgt agcataagca ccgtgttgat tatgcccaac tttatttcca
    20521 acaggtccaa agaaatgttg cattgcagga tttggattat caaaatctgc cacttctgta
    20581 gctttcccta cggtattatc atcgccaaat ttataagcat cgtaaagcaa aatatttcta
    20641 taaagttttt cacgtgcatt gtcgtctaaa atacgatacc aataatcgta gtgatctcgc
    20701 tgacgtttgg ctgtttcacg cgcattttct tcaacaaaat cattgagagc cttgcccgct
    20761 ttatggtcac tactgcggta gcgatcataa gctccaaatc ctagactaga catggtcgag
    20821 atgacaaata cggatctctc tggcaaggtc aggagaggca agaccatatt gcggtatttc
    20881 catgtggcac tcgtgatacg atcataaaca ccgatagaat acttggtgcc agctaaccct
    20941 tgcttcgttt tcacctcttc gatagtggat ttttcttcga caatgtaagc cttagtctct
    21001 gatttaaacc agtcattatt gcttgtattt ggtaaaaaga cttttcggta atgttccagc
    21061 gtgctaaaca aatctgtcgt tccatgttga ctggcaagac tgataccata agtatcgaca
    21121 ttattcttag ctagaagatt gttaaagcca gatttaccca actcaatcag agtatctaat
    21181 ggtgaagcat tccccttacc aaagaagtcc aaatggtaca gaactaggtc tttgacattc
    21241 acctgaccat agctaaagtt ataccaccgt tccagatagg tcaagccaag tagcaaggct
    21301 tccttgttgc gtttgatttt atctacaaga taaccttcag tgacggggtt agcactagcc
    21361 agtccagcat ccgctgacaa gagttttttc aaactgtctt ccagttgttg ttttgttttg
    21421 gcgaactggt cttctagata gagctcagtt tgcttgacgt ttggagaaat acccagcgtc
    21481 tttctgatgg cttctgaatg atagtcaacc ttttgtaagt caggtaagac ttgcttgatg
    21541 atagaggttt ggtcatacag gaattggttt ggcgtataga gaagtccagt attgcccaga
    21601 ctatattctg ctaatttggc gaaatcattc tggtatttga gatccagctt ctcagataaa
    21661 tcatccttgt agtgaagcaa gagtttgttt gcagtctgtt tgttagaaac aatgtctgtg
    21721 atgacttggt tgtccttcat catgactgct gacaagagtt ctttttgata taaaagactg
    21781 ttctcattga ccaggtttcc gtatttgacg atggttgcct tgttgtagaa aggtagcaat
    21841 ttttcaatgt ttttataagt caagttgcgc ttagcttgat aataggccac cttagaaaaa
    21901 tcactgtctt ttttgccact tgttgaaagt ggctccactg ttggtagaat gagaggattg
    21961 atttctgctt ttttgcttgc aatttgagaa gcatctagca ttgttcctct ttcttcaaag
    22021 gattccttgc tgacgacctc atccttgacc aaggtgacat tgtagactct gttggccttg
    22081 ctgctgaatg tgtcctttac cttcatttcg ttatagtggt aaccagtgat ggcatttccg
    22141 ttggttacat taacatcgct gagaacattg gtcaaacttc cagcatgcct aacatcacca
    22201 gaagttcgat cccacaaatt gcctgccact ccagcgactc taccaaagtg cttgacattg
    22261 ttgatatcac cttcagcata gctatcttgg atctgtgcat ctcggtctac taggcctgca
    22321 agtccaccca cagtctgatc tgaagtattt gtgttagatg aaatggctac tgtcgctttt
    22381 gacttagtaa gtaaagcctt gtcacctgtc aaatgaccga ccataccacc gatattgtag
    22441 gcagcagtcg tttcataagt gttgataatt cttcccttga aactgctctc tgtgatgctt
    22501 gattgctcag ccttagccag caaaccaccg ataccacgtt caccagccag aacaccatcg
    22561 acgtgaactt gcttaatttt tgtgttattc tgagcttcat ttgccagtga accgatatca
    22621 tctttccctg aaatagcaac attttttaga ctcagttttt ctactgtagc accactcaag
    22681 ttttcaaaca gaggtttttt caaattatag atagcataat tcttgccatc tttttcaccg
    22741 attaaacgac cagtaaaggt gtccttgata taggatcttt catcaggacc aagctccact
    22801 tcgttagcat tcaggctggc cgctaaatga taggttccag agggattttg gtttatagct
    22861 ttgaccagat tactaaagga agtaaagttt gttgtttctt ctgttccctt cttagctaga
    22921 tagaaggtaa aattatcttt atatctgctt tctatctcct gctgaagctt ctctactttt
    22981 gctgtgattt tataaaggat tttatcattt tttctttcct ctgatattga tgctactggt
    23041 aggtatacat ctttgaatga agaagatttc actttaacaa agtagctatt tggattgctt
    23101 ggaacttgct ctaacgaaat gtgttgttta taagtaccat ttgacaaact gtataactct
    23161 aggtcggaaa catttcttaa ttcaagtgtt ttctctggtt cttctacctt tttatcaggg
    23221 tctagttcat tttcttgttt aatttcttcg tttccatttg aattggatgt gtttgattcg
    23281 gttgaaacat cctcagttga atttccgttt gatggttctg gttctgtttg tccattctct
    23341 gatgttgtat tacctgaatt ttctggtttt gttgcagttc cgtttttttc tggtttattt
    23401 gattcttcaa ctggtggttt tgaatcacta ggtttattgg atacttctcc agtattttcg
    23461 ttagctattt tcccagagtt tgtttgtgtt tcttctgcag gttgaactgg tttttctgtt
    23521 tcttgatttg aggtaccttc tactgtgcct tcatttggat ttactggaac ttcttctaca
    23581 gttttttctg aattttcatt tttagagtca ttatgttctg gtttatttga ttctccaact
    23641 gaggttgtcg aatcactagg attactggac acttccccag tatttttgct agatgtatct
    23701 ggtgatactt tctctgaatt cgttgttgat tcttctgcag gttgaactgg attttctgct
    23761 tcttgaattg aggttccttc tgtagtacct tcatttggat ttactggtgt ttcttctgtt
    23821 ggttttactg gaacttcttc agttttttct ggaccttgtt ctttggtctt ctcaaccgga
    23881 gtttcaggtt ttacttgctc aatattaccc ttatattctg gaagcggtgc tacctgctct
    23941 ggttcacctt tatcacttac cacagtatct ggcgactctg gttgaacctc agtctcacct
    24001 ttgtcggtca caactgcttc gggtaatgta ggttgaactt ctggttcgcc tttgtcactt
    24061 actacagctt cgggcaactc aggctgaatt gcgggttcaa caatagctcc agactgtacg
    24121 tccttatgtt ctacaccagt ctcaggttgt tcctttataa cttgagtttt tttagtacct
    24181 ttttcgacta ttcttggact aggcgcagtc gttgaagttg aaacaatttc tcgcgaaact
    24241 tcttccttgt ttacagagaa tattctgacg atttcaactt tcttacctaa tttaccttct
    24301 tgttttactc ttacagttcc ttcagctaaa tcaggatttt cttgaatttc ttcttgaaaa
    24361 tctatttttg tctccatagt ttcctcacga tataagagtt caggtttgtt caattgacct
    24421 gataaaactt catcctgtgg atttaatgta tttaccccag tcttttcttt tggagaaatc
    24481 ttctcctctt tcttcgtttc tagattctta tgttcggcta attgttcttg agaatctgaa
    24541 gattgtttct cttcttttct tggattgatt aattcagtag agaaaggttt ttcaactact
    24601 tgaacttctg tcggcttagt tgaagaaaca ggtgtttgtt cctgaatagc ttgtactgtt
    24661 gatggatggt ctacaaaatt cggtgtaaca ttataatcca ccttttgttg ttttgtagga
    24721 gtggcaactg aactcttttg attacttact tcagactcag aagtcgtttt tccctctttg
    24781 atatatccaa tataagtgta acctgaaatc tctttaggaa gaggtaattt ttctccagag
    24841 gtcaattcat agtccgtatt gtaatttagc aaaagatgat tttctaaagc atggactgaa
    24901 actaagacac catttcctat ccctgcaacc aatactaaat gtaataccgt tttattctta
    24961 acctttttct tggaaacagc aaaaattaaa attcccatag cagctaagct agcaccagca
    25021 actagggctt gcctctcatt cttgcttcca gtatttggca attccgccag ttgattttga
    25081 gaatttaact tataaacaag ataataagtt tcatcatcat tctccacgta tgtcggaata
    25141 tcatagacaa gctgcttctt ttcttctgat gatagctctg aatctgccac atatttatag
    25201 tgaactcccg cagtttcttg agcatccaca gatgaactag ctaatacaga cataaaaaat
    25261 aaacttgaaa tcgttgcaga tacaagtcct actgataatt ttctaaatga aaaacgctct
    25321 tgtttttcac caaaatactt ttccattatt cctccttgaa ataaaattta tatatgttac
    25381 aaagaccttt attatattag tgtattatct attatctata gaaaaggcag tataccttaa
    25441 ttatactctt aatttacaaa aaagtcttaa aattgagatg cgctttcata ctttgtttta
    25501 tattatttgg aggtacaata acacctacca tgaaatttac acggtaggtg ttactcatat
    25561 cactaatcgt tctaaaaatg gtttgaggca gttgaggaga attccttcta tccagcttcc
    25621 ttgtgctgat gagcgatggt cttcctgcag gctttttttt agaaaatctc ggacttgttc
    25681 tggtgcgatt tcaaattcaa aggctttcat tttatagaaa aagtcgatga gatgatctga
    25741 caggtattca gttgaaaagg gtacttcacc actttttcta tattctaata agagtctaga
    25801 aaatcgagct ttttcttcag gaagctcacg aaaataggaa ttgaggatcc aagtctgctt
    25861 ctgttttctt tcaattggat cctgactggc aattcgttgg tctttttcca gctctttttg
    25921 gtattgtttg gccttgatag ctcgttctgc tctattttta ccaaaaagaa ttttttccca
    25981 cttgcgttct tcttgagtca gggtctctgt aaagccaaag taatcttgat aagcacgctc
    26041 tgcgggtccc atggctagaa ccagattgtc tgcatattgc ttggcgattt tatccctctt
    26101 cttgcgttct ttctctgcct ggatacggag ttcttgttcg tagtcaattt tctccttgcc
    26161 tagcttgaca aggtagagtt ggtcatccga tttcccaagt aaaaagggtt tgatacactt
    26221 ttcaaggact tcttccatcc gagccttttt ctttggttcc gccttggtcc aacttcctcc
    26281 ctgaaagact tctaggaaaa gctggtagtc tctctcaggc gcaaattgat tgccacgatt
    26341 gggtttgaaa acaccttttt cccagagcca ttttagaagt cgctcgtcaa agttactttt
    26401 attgaccttg attttttcct ttttctgagc ttttctggtt agattttcaa cctttctgag
    26461 cagtttttct tcctcttcca attgctggtc aagggacaat cgatgaaaat gacgaacaca
    26521 gtcgctacca attggaaaga ggcgttggct tgtgacaccg ttaaagagtt cataagcgta
    26581 tttgatggca tttccacaga cacaattgct acggccgata ccgttaaaaa taaaggaaac
    26641 ttcattccat tccttggtag cttgttccca agtatccgct ttcgaagcct gtaaaactgc
    26701 atcgtgcagg gattttctaa ctggaagtgt catgaggtct cctttctaat actcaataaa
    26761 aatcaaagag caaactagaa agctagccgc aatcagctca aaacactgtt ttgaggttgt
    26821 agatagaact gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga
    26881 agtcagtaac catatataca gcaaggcgaa gctgacgtgg tttgaagaga ttttcaaaga
    26941 gtataagtta tacttttaca acttgaacct cgtctttacc gagtaaaatc aagtattttt
    27001 caatattttc aatcgaatag gctcgtgata aagcctcttc gtatagagct aactgaccac
    27061 gatagcggtc tacgagttga cttggttcat catagcggtc tgtcttgtag tcgaacagaa
    27121 caattttgtt ttcgtaaagc agatagccat caaggatacc acggacaaca aagtcttcct
    27181 gactcttttg gtctcgtttg agcatggaga aaggttgctc gcgataaaga tggtcggtat
    27241 tagcaagaat ttcctgaccg agtactgtgt caaagaaagc aagaatttta tcaagattga
    27301 tcttgtctct gacagcttgg ctagtttgaa cttgtttgag tgtttctgtt aggctagcaa
    27361 gggttagttg ctggctgagg tcaattctct gcatgagttc gtgagtagca ctaccaatct
    27421 cagctccagt taccttttct ttggttgaaa aatctggcaa atcgaagctg attttcttgc
    27481 ctactgactg accttgacca gcaatctcga caccttccat atccataact ggttcgtaga
    27541 atttcttgat ttgacttggg gtttgaacac taggaagttc aatagctgcg cggtgaagag
    27601 tattataaac ttccacctcc ttcagcattt ccagagcttc tttgatggta tctgactgac
    27661 gattgtctgc ttgggagcta tcttggagag gactcttggt ttccaactct ccgatagctt
    27721 ctctggtcaa ctgatcttcg ccaataaaac gataactaaa gttgagcttg tccttagtaa
    27781 acactttact gatagcccaa agccaatctt ggaaattccg tgcttgcagt ctagtattgc
    27841 tatttagttt cccatttttg gctgctgggt attccttgga ttccagcttt tcacgagaac
    27901 ccttgccgac aagatagagc tttttctcag cccgcgtcat agcaacatac agcaaacgca
    27961 tctgctcaga atagcttgct agctgtaatt cctcttcgtt ctgcctatag gtcagactag
    28021 gaatggagag tttgatggtt ttaggatagt ggtcttctac tgcccctgtc tccatcttgg
    28081 caatatattt gacaccaaga ccattctgac gactgagaat gacttctgac atagagtctt
    28141 gcttgttgaa atcttgatcc atattgagga taaagacgta aggaaactcc agccctttac
    28201 tcttgtggat ggtcatgagc tctactgcat cttttggcgg tgcgacggcc acgcttgcca
    28261 aatcgtgctg ggcttctaag acttggtcaa tcatacgaat aaaacgcgac aaacctttga
    28321 aattgctctt ttcaaattga tcagcacgca gtgctagggc atagagattg gcctgcctag
    28381 caggaccatt cggcaaagcc ccaacatagt cataataaaa acggtcgttg taaatcttcc
    28441 aaatcaagtc atagagagag tgggttttgg catacaagcg ccaagaagct aggatatcca
    28501 tgaattgctt tagtttttca gctagagctg tgtgaatcaa gcctttttga ctacttgcca
    28561 ttttttgtgc attgaccagt ttctcataga gattttcgtg gactttatcc tctgctttct
    28621 gaagggacaa acgtgctagc tcatcctcat caaaaccaaa cattggagac ttcataaggg
    28681 caaccaaggc gtagtcttgc aggggattgt gaatgacacg aagagtgtct agcatgactt
    28741 gcacttctag ggattggaga taattgtttt gctctccgtc agttttgaca ggaattccgt
    28801 actcagacag ggcgaggaga atctggtcat tacgactgcg gctggaggtc agaagggcaa
    28861 tttccttaaa ggcaacacct ttttcttgat gaagtttcag aatctccttg ataactaagc
    28921 gcatttcgcc tgttagtttc gtttctgttt gactctcttc ttcctcacct gtatcgtcct
    28981 tgtcgtagag gagaaatgct gccttgttgt ctggattggg agtcagtttg gtattggcaa
    29041 aaacaagctg gtgcttgtta tcatagttga tttcgccgac ctcttggtcc atgagacgtt
    29101 caaagacatc attggttgct gacagcactt ctgaactact acggaaattt tccttgagga
    29161 taatgagcct gccttcttgg ggattttgcg catagcgttg gaatttctca ttgaaaatct
    29221 gcgggtctgc ctgacggaaa cgatagatgg attgcttgat atctcccacc ataaagcgat
    29281 tgtggccatt agacaacaat tccagcatcc gttcttgaat atggttggta tcctgatact
    29341 catcgaccat gacttcatgg aagcgctcct gataagactc acgaacttgt gggaaattct
    29401 ctaaaatctc aatggtgtaa tggctgatat cagcgaattc gaaggcattt tcctgtcgtt
    29461 ttctctgacg ataagcctct acaaaatcgc tcatgaaaga ttggaaggtt ttagctagtt
    29521 tccaagtgtc tccatgataa cgttcttgat agtcgagaat cgctatctgg tctgataatt
    29581 gtcctagttt agcaaactgg gtctttctct cttcgttgta ggcatcagcc aggggcttca
    29641 aatcagcctt acggctggca ttagtcagag ctcgaccgtt tttctcctta gagatggcga
    29701 caacacgcgc aagcactgcc tgataagcct gactatcgga ctcctgattt agggagccaa
    29761 tttcatccag aattaactga acattttcta aataggcagc ctttgcaaac tccttggcat
    29821 cgttatccag atggtaacgg aaaaagcttt ccaaatccca aagggcttgt ttgatttgct
    29881 cggtcagttt ttctttttca ctggtaaaat cagctttctc aaatcctttg aggaaagatt
    29941 cactcagcca cttttgagga ttactggtgg attggaggaa gtcatagatt ttatagacct
    30001 gctggcgcag accccgttcg tccttgccac gcccagcaaa gtttttcagc aaatgactaa
    30061 aggtctcttt ctgtttacct tggtaatgcg cttcaaagac ctcatgaaag acttcgtttt
    30121 cgagaataag ttgctcgctt tggttttgta aaatacggaa attaggtgca atatcaagca
    30181 gataaccatg tttgccaagg aatttttgtg tgaaagaatc catggttcca atggcagcgt
    30241 tgggtaggtc tgccaactgg cgacccaagt gttgtttgag gtcgacatca tctgtttctt
    30301 ggattttctt gctgattttt ttctctaaac gttctttaag ttcagttgca gccttgacgg
    30361 taaaggttga gataaagagt tgagaaattt cgacaccacg cgccaattgg tccagaatgc
    30421 gctctgccat gacaaaggtc tttccagaac cagccgatgc tgagaccagg atattctggg
    30481 cagaagtgta gatagcttcg atttgctcgg cagttttctt ctgttccttg ctcgaatttg
    30541 cttctgcttc ttgcagtttt tgaatctcct cctcacttaa aaagggaata agcttcatcg
    30601 attcaactcc tctcttattt tttcaagcca agcttgcttg agtttttctc cgaccagacg
    30661 cttgccatca gctaggtcca acttttctag gaaacgggct tggcccagat ggtaattggc
    30721 ttcaaagcct gtaatagcct gatgttgctg gacgtatggg gcaatgcttc tgccattttc
    30781 agtataagga ttgatggcga accggcctgc taaaatcttc tcagcagctt tcttgtaaag
    30841 ataggcattg tagtccagta ggagctgaaa ttcctcatct gtcagttgat tagccttgtt
    30901 tttgttataa aattcgccta aataactgct ttctttttcc aagaagagcc cttggtattt
    30961 catagatttg ctggcttcta ccactgctcc tgccagactt tttaccgcca tcagagattg
    31021 gacaggttca gccatttcca agtacatggc gccgaaaaag ttctgctccc cttctctttt
    31081 tagggcagca agataggttg gtaactgaga attgagccca ttaaagaaat gaggaaactg
    31141 gaactgagtc agactggatt tgtagtctac tactcctatc gctccattag ctttcaaacg
    31201 gtcaatccgg tccaccttgc ctcgtacaaa gacactgcgt ccattgtcta attgaataaa
    31261 ggcttggtct tttccaccaa aatttgcttc ttctttgatg gtttcgatgg ctggattgtg
    31321 tcggagaata tgtccagttg tccgtgcaac atcaagcaaa acttccttgg taaactgggc
    31381 ttccaaactt tcttgataaa tagcttcaaa ttcgcgttct tgactggttt cttgaatagc
    31441 ttgttctaga cgttggtcaa aggaatcttc attaggcaac tgtaaggcgc gttcaaagat
    31501 acgatgcaag aaattcccgt gactacgggc atcaggatgc aaacgtaatt cctcctgcaa
    31561 gcctaaaacg tagcgtagga aataactgta ttcattgcga taaaactctg tcaaacccga
    31621 cgtagacagg taaaactcct gtttggcagg atagagagct tgcaaggtgt ccttggctaa
    31681 ggtcttgctg cttggactgg ttgggatagc tggattttcc agaccttgct gatctagttt
    31741 tttacctatg acacgcgaca gaaccttgac aaaagtcaaa tcttgctcag tatcgctcat
    31801 ctcaccctgc tggtgatagg caaccagact agacaaaaga ctgtgatagg accccatatc
    31861 ctccttagac agtcctttgt gattcatcct cttctctctc cgcctaaatc caaaatggat
    31921 caactcttga agataggcag attccttact ttcactttcg ttaaaaaggc ttggagccga
    31981 caagaacaac tgcttacgag cagaattgac caaggaaagc atagtgtagc gatttttctt
    32041 gagattttca ctgctggcaa tcagtaattg aacgccttct tcggtcgctt ggtttaggtt
    32101 ttgcctttct tcatctgtca gaagactggt gttttgagaa atttttggta aattgtcctg
    32161 agttagtcca atagcataga caaagtcagc agtcaatggt gcaatcaaat cgtaactctg
    32221 caccagaaca gtgtccactg ttgctg
//
LOCUS       DBDESG010000021        31470 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.021, whole genome shotgun sequence.
ACCESSION   DBDESG010000021 DBDESG010000000
VERSION     DBDESG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 31470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.021"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..663
                     /locus_tag="ACKB38_000929"
     CDS             <1..663
                     /locus_tag="ACKB38_000929"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGL7979718.1"
                     /translation="NGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINE
                     FVSDTEAEIAALKELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLY
                     DNDLSVQEKIEKIVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDN
                     PNALGAPENFEITIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLG
                     LF"
     gene            991..2166
                     /gene="mutY"
                     /locus_tag="ACKB38_000930"
     CDS             991..2166
                     /gene="mutY"
                     /locus_tag="ACKB38_000930"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGL7979719.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQVYAQANLDSSQD"
     gene            2222..2926
                     /gene="yycF"
                     /locus_tag="ACKB38_000931"
     CDS             2222..2926
                     /gene="yycF"
                     /locus_tag="ACKB38_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGL7979720.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2919..4268
                     /gene="vicK"
                     /locus_tag="ACKB38_000932"
     CDS             2919..4268
                     /gene="vicK"
                     /locus_tag="ACKB38_000932"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGL7979721.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQV
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            4270..5079
                     /locus_tag="ACKB38_000933"
     CDS             4270..5079
                     /locus_tag="ACKB38_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGL7979722.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            5170..5412
                     /gene="relB"
                     /locus_tag="ACKB38_000934"
     CDS             5170..5412
                     /gene="relB"
                     /locus_tag="ACKB38_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGL7979723.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            5414..5668
                     /locus_tag="ACKB38_000935"
     CDS             5414..5668
                     /locus_tag="ACKB38_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGL7979724.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            5743..7599
                     /locus_tag="ACKB38_000936"
     CDS             5743..7599
                     /locus_tag="ACKB38_000936"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HGL7979725.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKDGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVTQHNSR
                     KTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            7764..8612
                     /locus_tag="ACKB38_000937"
                     /pseudo
     CDS             7764..8612
                     /locus_tag="ACKB38_000937"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(10824..10937)
                     /locus_tag="ACKB38_000938"
     CDS             complement(10824..10937)
                     /locus_tag="ACKB38_000938"
                     /inference="COORDINATES: protein motif:HMM:TIGR03807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979726.1"
                     /translation="MLPTDIFRKIAKYKISGNVIDNLRRSKSINLLFQNMF"
     gene            complement(11328..12314)
                     /locus_tag="ACKB38_000939"
     CDS             complement(11328..12314)
                     /locus_tag="ACKB38_000939"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGL7979727.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            12508..14976
                     /gene="gyrA"
                     /locus_tag="ACKB38_000940"
     CDS             12508..14976
                     /gene="gyrA"
                     /locus_tag="ACKB38_000940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGL7979728.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            14976..15719
                     /gene="srtA"
                     /locus_tag="ACKB38_000941"
     CDS             14976..15719
                     /gene="srtA"
                     /locus_tag="ACKB38_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGL7979729.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
     gene            15963..16760
                     /locus_tag="ACKB38_000942"
     CDS             15963..16760
                     /locus_tag="ACKB38_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGL7979730.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            16908..18167
                     /locus_tag="ACKB38_000943"
                     /pseudo
     CDS             16908..18167
                     /locus_tag="ACKB38_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            18263..19537
                     /locus_tag="ACKB38_000944"
     CDS             18263..19537
                     /locus_tag="ACKB38_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGL7979731.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            19547..20425
                     /gene="truB"
                     /locus_tag="ACKB38_000945"
     CDS             19547..20425
                     /gene="truB"
                     /locus_tag="ACKB38_000945"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGL7979732.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            20666..20968
                     /locus_tag="ACKB38_000946"
     CDS             20666..20968
                     /locus_tag="ACKB38_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGL7979733.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            21076..21714
                     /gene="udk"
                     /locus_tag="ACKB38_000947"
     CDS             21076..21714
                     /gene="udk"
                     /locus_tag="ACKB38_000947"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGL7979734.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            21842..23182
                     /gene="xseA"
                     /locus_tag="ACKB38_000948"
     CDS             21842..23182
                     /gene="xseA"
                     /locus_tag="ACKB38_000948"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGL7979735.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            23160..23372
                     /locus_tag="ACKB38_000949"
     CDS             23160..23372
                     /locus_tag="ACKB38_000949"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGL7979736.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            23369..24244
                     /locus_tag="ACKB38_000950"
     CDS             23369..24244
                     /locus_tag="ACKB38_000950"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGL7979737.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            24237..25052
                     /locus_tag="ACKB38_000951"
     CDS             24237..25052
                     /locus_tag="ACKB38_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGL7979738.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            25045..25476
                     /locus_tag="ACKB38_000952"
     CDS             25045..25476
                     /locus_tag="ACKB38_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGL7979739.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            25483..27150
                     /gene="recN"
                     /locus_tag="ACKB38_000953"
     CDS             25483..27150
                     /gene="recN"
                     /locus_tag="ACKB38_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGL7979740.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSYLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            27152..27880
                     /locus_tag="ACKB38_000954"
     CDS             27152..27880
                     /locus_tag="ACKB38_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGL7979741.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            27959..29782
                     /gene="lepA"
                     /locus_tag="ACKB38_000955"
     CDS             27959..29782
                     /gene="lepA"
                     /locus_tag="ACKB38_000955"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGL7979742.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(29863..30060)
                     /locus_tag="ACKB38_000956"
     CDS             complement(29863..30060)
                     /locus_tag="ACKB38_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979743.1"
                     /translation="MKIKEQTRKLAAGCSKHSFEVVDETDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTWFEEIFEEYKN"
     gene            30240..30715
                     /locus_tag="ACKB38_000957"
                     /pseudo
     CDS             30240..30715
                     /locus_tag="ACKB38_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            30753..31055
                     /locus_tag="ACKB38_000958"
     CDS             30753..31055
                     /locus_tag="ACKB38_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979744.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            31063..31470
                     /locus_tag="ACKB38_000959"
                     /pseudo
     CDS             31063..31470
                     /locus_tag="ACKB38_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         9767 a         5535 c         7097 g         9071 t
ORIGIN      
        1 aatggtggtg tggctaaaga cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt
       61 tttgctaact tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc agcagttgta
      121 gctattaacg aatttgtatc tgatacagaa gctgaaattg cagccttaaa agaactctgt
      181 gcctcaatcg atgtaccagt tgaattggct agtgtctggg ctgatggagc agaaggtgga
      241 gtagcacttg ccgaaacagt tgttaagaca attgctgaaa atccagctaa ctataaacgt
      301 ttgtatgata atgacctttc tgtccaagaa aagattgaaa aaattgtcac tgaaatctat
      361 cgtggtagca aagtgaactt tgagaagaaa gctcaaacac aaattgctca aatcgttcaa
      421 aacggttggg acaaattgcc aatctgtatg gctaaaactc aatacagttt ctcagacaat
      481 ccaaatgcgc ttggagcacc agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa
      541 ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc aggtcttcca
      601 aaacgtccag cagctctcaa catggatgtt gaaagcgatg gaactgtact aggcttgttc
      661 tagtggattg aaattgactt taagtgagtt tggaaataat atccaagctc attttttctc
      721 agactgcaag cagttcatca ataagattta tactcttcga aaatctcttc aaactacgtc
      781 agcttcacct tgccgtaggt atatgatact gactctgtca gttctatcca caacctcaaa
      841 acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
      901 taagtagata gaaaagttat atactatcta ttgaaaatat tataaatcag atacaaagac
      961 aggtgaaacc ttctgtgtta tactagagat atgttagatt tgaaagaata cggtatcgtc
     1021 atgtggccgg aggagaaagt catttctttc cgtgagaaac ttctcgcttg gtatgatgaa
     1081 aacaaaagag atttgccttg gaggagaagt aaaaatcctt atcacatctg ggtatctgaa
     1141 atcatgcttc agcagaccag ggtggataca gttatccctt actacgaaag atttttggac
     1201 tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg agagtttact gaaagcttgg
     1261 gagggcttgg gctattattc tcgagttcgc aatatgcagg ctgcagccca gcagattatg
     1321 actgactttg gtggccaatt tccaaatacc tatgaaggaa tttccagctt gaaagggatt
     1381 ggaccttaca cagcaggagc catttccagt attgctttta acttgcctga gccagctgta
     1441 gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag tcaaccacga tattgggatt
     1501 ccaagtaatc gaaaaatttt tcaggcaatg atggagatct tgattaatcc ggatcgtccg
     1561 ggtgatttta atcaagcctt gatggactta gggtctgata ttgagtctcc tgtaaatccc
     1621 agacctgaag aaagcccagt caaggacttt agtgcggcat atcagaatgg aaccatggac
     1681 cgttatccaa ttaaatctcc taagaaaaag ccggttccta tttatcttaa agccttagtt
     1741 gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg aaagcgaaaa gctcttggca
     1801 ggtttttggc atttcccctt tatagaagtt gataactttt cgcaagaaga gcagtttgac
     1861 ctctttcatc aggttgcgga agaaagtgtg aactttggtc ccagtccaga agagagtttc
     1921 cagcaggact atgacctaga tgttgattgg cttgatgttt gttttgatac agtccagcat
     1981 gtttttagtc atcgaaaatg gcatgtacag attgtagcag gtcaggtgag tgacttccat
     2041 gatttttcag atagggaagt tcgctggctt tcaccagaag aattcaagaa ttacccactt
     2101 gccaaacccc aacaaaaaat ctggcaggtt tatgcacaag ccaacttaga cagtagccaa
     2161 gactagctat tgtgtcttct gactattttt ttggtataat agtagagaaa aaggtgaaca
     2221 tatgaaaaaa atactaattg tagatgatga gaaaccaatc tcggatatta tcaagtttaa
     2281 tatgaccaag gaaggttacg aagttgtaac tgcttttaat ggtcgtgaag cgctagagca
     2341 atttgaagca gagcaaccag atattattat tctggatttg atgcttccag aaattgatgg
     2401 tttagaagtt gctaagacca ttcgtaagac aagcagtgtg cccattctta tgctttcagc
     2461 caaagatagt gaatttgata aggttatcgg tttggaactt ggggcagatg actatgtaac
     2521 aaaacccttc tccaatcgtg agttgcaggc gcgtgttaaa gctcttctgc gtcgttctca
     2581 acctatgcca gtagatggtc aggaagcaga tagtaaacct caacctatcc aaattgggga
     2641 tttagaaatt gttccagacg cctacgtggc taaaaaatat ggcgaagaac tagacttaac
     2701 ccatcgtgaa tttgagcttt tgtatcattt agcatcgcat acaggtcaag tcatcacgcg
     2761 cgaacacttg cttgagactg tctggggtta tgactatttt ggtgatgtcc gtacagttga
     2821 tgtgactgta cgacgtctgc gtgagaagat tgaagatacg cccagccgac cagagtatat
     2881 cttgacgcgc cgtggtgtag ggtattacat gagaaataat gcttgattta ctgaaacaaa
     2941 ccatttttac cagagatttt atctttatcc tgattttgtt aggtttcatc cttgttgtga
     3001 ccctcttatt actggaaaat agacgtgata atattcagtt gaagcaagtc aatcaaaagg
     3061 ttaaagattt gattgcagga gattattcca aggttcttga tatgcaaggt gggtctgaaa
     3121 tcaccaatat taccaataat ttgaatgact tgtcggaggt tattcgtctc actcaggaaa
     3181 atctagaaca agagagtaag aggctaaata gtattctgtt ttatatgaca gatggggttc
     3241 ttgcgactaa ccgtcggggt cagattatca tgattaacga tacagccaag aagcaactgg
     3301 ggttggttaa ggaagatgtt ctgaatagaa gcattttgga attgctcaag atagaagaaa
     3361 actatgaatt gcgtgatttg attacccaaa gtccagaatt gttgctagat tcccaagata
     3421 tcaatggcga atatttgaac cttcgagttc gctttgcctt gatacgtcga gagtctggct
     3481 ttatttcagg tttggtggct gttttgcatg atacgacgga gcaggagaag gaagaacgcg
     3541 aacgaagact ctttgtttcc aatgttagcc atgagttacg gactcctctg actagcgtaa
     3601 aatcctatct tgaagccttg gatgaggggg ctttgtgtga aactgtagca ccagacttta
     3661 tcaaggtttc tcttgatgag accaaccgta tgatgcgcat ggtgacggat ctcctccatc
     3721 tttcacgtat tgataatgct accagtcacc tagatgtgga actgattaac ttcactgctt
     3781 ttattacctt tatcctcaat cgttttgaca agatgaaagg acaggaaaag gagaaaaaat
     3841 atgagttggt gagagattat cccatcaatt ctatctggat ggaaattgat acagataaga
     3901 tgacgcaggt tgtcgacaat attttaaata atgctattaa gtattcgcca gatgggggta
     3961 aaatcactgt cagaatgaag acaactgaag accagatgat tttatccatt tctgaccacg
     4021 gtttggggat tcctaagcag gatttaccac gtatctttga ccgtttctat cgtgtggatc
     4081 gtgctagaag tcgtgcacaa ggtggtacag gtctaggact gtctatcgct aaagaaatta
     4141 tcaaacaaca taagggcttt atttgggcca agagtgaata cggcaagggt tcaaccttta
     4201 ccattgtact cccttatgat aaggatgcag tgaaagaaga agtatgggag gatgaagtag
     4261 aagactagaa tgagtgaaat aggctttaaa tacagtattt tagcgtcggg ttccagtgga
     4321 aattcttttt atctggaaac ctcaaaaaag aagcttttag tagatgcagg cttgtctggc
     4381 aagaaaatta ccagtctgct agctgaaatt aaccgtaagc cagaagacct ggatgccatc
     4441 ttgattaccc atgagcattc agatcatatc catggagtag gcgttttggc tcgcaagtat
     4501 ggtatggatc tttatgccaa tgaaaagacc tggcaagcta tggaaaatag taaatatctt
     4561 ggcaaggtgg attcttcgca aaagcatatt tttgaaatgg gtaaaaccaa aacctttgga
     4621 gatatcgaca tcgagagttt tggtgtaagc catgatgcag tcgcaccgca gttctatcgc
     4681 tttatgaagg atgataagag ttttgtcctc ttgacagata caggttatgt cagtgaccgt
     4741 atggcgggca ttgtcgaaaa tgcggatggt tatcttatcg aggccaacca tgatgtagag
     4801 attttgcgat caggttctta cgcttggcga ctcaaacaac gaatcctatc tgaccttggt
     4861 cacctttcta acgaggacgg tgctgaagct atgattcgga cgctaggaaa tcgtactaag
     4921 aagatttacc ttgggcattt atctaaggaa aacaatatca aggaactggc tcatatgacc
     4981 atggtcaatc agctggctca agctgatctg ggagtcggag tagactttaa ggtttatgat
     5041 acctcaccag ataccgcaac accattgaca gagatataga aagaacgctg agaaggtgtt
     5101 ctttttatat tgactgaaca cctaaaaagt aatacaatgg tgttaccatt aaaaaaggga
     5161 gcacaaaaga tgactactat tacattaaaa gtttctgaag ctgataaaac atttatgaaa
     5221 gcaatggcta agtttgaagg agtttccctt tcggaactta ttcgcaccaa aactcttgaa
     5281 gccctagaag atgaatacga tgctcgtgtg gcagatttag cctatcaaga gtatttagaa
     5341 gacttggaaa aaggagttga acccattact tgggaagaaa tgatgcatga tttaggcttg
     5401 aaggatgaat aatttgtata aattagttcc aacaagacgt tttatcaagc aattgaaaaa
     5461 attggaccgt tatacgcaga agctaattac aaactattta caaaccaatg ttttggaaga
     5521 cccaagacga cacggaaagg ctttggttgg taatcgcgtt ggtcaatggc gctatagaat
     5581 tggtaattat cgagttatcg tacaaattgt agatgatgaa ttagtcgttg ctactctaga
     5641 agttggtcat cggagagata tttattgaat tacttttttc aagtaaattt tcattcttta
     5701 aaatatgata taatagggct tacaatataa gagaggttgc tgatgacaat agatattagt
     5761 gaagaaagtt tggctaaaga atcggctgac ttattaaaaa ttcttttaaa agatcgaacg
     5821 acaaagaaat caattgtttg ggctacgcat tcttatgaat tgttaggaaa gggatttgct
     5881 ccaagtgatc gcatcaatcc aagtaaggtt actggaaact ttgccaactt gattcagccc
     5941 agatctgaaa aatccaaata tgaacaaaaa gatcggacaa aaataagggc agaagtgttt
     6001 acaccaacgt ggttggtcgc aaagcaaaat ggatatgtag aatcgaaatt aggctcgtta
     6061 agtcttgagg agtatgtgga cttacgatgg ttagaggtaa cttgtggtga agcaccttat
     6121 atggtaacga gatatgatac cgtaactggt gaggaaattc cattatctga gcgagttggt
     6181 tttgttgata gaaaactgca acggattagt cgtgaggttt cagatgaagt cacattttat
     6241 aaacttgtaa aagatgctta tcatgcttcg tatggttatg agtatcaagg agattctctc
     6301 cttttggcgc gtgagaatct tttggcaact tttgaggact attatctagc taagactgga
     6361 aatcaaccaa cattagagca aaagaaagaa attgcgacta ttatctctta caatgtcttt
     6421 cagatggatg gattgaaaaa aagtagtcct tattcagcta aacaaaagca atctcaacaa
     6481 ttgagtttgt ttgcggatga gctagaagtc caagaagtag aagaatctaa aacccatatc
     6541 aaagactgga aaaagaatag aatgataggt tttgagcgcc tatctagcga ggaaagtgaa
     6601 atgaaatttg atgtcgtgat aggaaatcct ccgtatcagg aaacaggaga agccagagat
     6661 gagccgatat atcactattt tattgatgaa gcttataaaa tcgcagataa atctatacta
     6721 atcaccccag caaggttttt atttaaagct ggtcaaactc ctaaaaattg gatggaaaag
     6781 atgcttgaag acaagcattt aaaagttcaa ttctatgaac ttaaaagcgg taaagtattt
     6841 acaggaacgg atattaagga tggagtagca ataacttatc gtgatgttga taaagatttg
     6901 ggaccaatcg gagtatttac tatttttgaa actttgaatg gtattgtaaa aaaaatcgaa
     6961 aaacatccag aatttgaaac tttttctgat ttagtttatc ctcaaggtat ttatagattt
     7021 tcaaataaac ttttttctga ttatccaaat gctgaaaagc tgcaaggaaa agggacaaaa
     7081 aataaaattg tttcgaaatc actaactcag ttagattttg ttttcaaaaa caacgaagaa
     7141 aaagaccatg ttgctctact tggaagaatt ggctccgaac gtgtttatcg atatattaat
     7201 aaaaaatatt tagatttacc ggaaacattc gaaaattata atgtttttgt accagaagct
     7261 aatggaagtg gtgccttagg tgaagtctta tcaacacccc taatcgggca tacagatact
     7321 tttttatcta ttggtaattt taaaacaaaa tttgaagccg atgcttgtat taaatttatt
     7381 aaaactaaat tcgctagagt attattaggt gttttgaaag ttactcagca taattcacgc
     7441 aaaacttggt attacgtccc cctccaagac tttacggtca attcggacat tgattggaca
     7501 caatcagtga ctgatattga ccgccagctt gatcaaaaat atgacctttc ccctgaagaa
     7561 attgccttta ttgagaatca tgtaagggag atggattaga aaagtatttt tatttgacaa
     7621 atagtgctca atgatctaaa atgactatat aggattaggt caggaagcat acgatgccct
     7681 gacccttttt gtacttatga gatgagaaag tcatttgtta gataaattga cttcgttagc
     7741 aaacgttcaa aaaaggaaaa cttatgccag tagaaattaa aaccactaaa gaaattcatc
     7801 ctaaaatcta tgcctacacc acaccgacag taaccagtaa tgaaggctgg attaagattg
     7861 ggtatacaga acgtgatgtc acacaacgta tcaaggagca aacgcataca gctcatatag
     7921 ctacagatgt cttatggact ggtgatgcag cttatacaga agagcctgat aaggggaaaa
     7981 ctttcaagga ccatgatttc caccatttcc tttctttcca tgatgtagaa cgtcgtccca
     8041 agacggaatg gttctatttt aatggaactc ctgaaaaatc aaaaaatctt tttgataagt
     8101 ttgttcagca tgatttgtct ggttatcagc ctggaaaagg acaggactat actctgcgac
     8161 aagagcaaga agaagcagtt gctaagacat tagcttattt ccaagaacat gctggaggca
     8221 agtttctctg gaatgccaag ccacgctttg gtaaaacctt gtctacctat gacctagctc
     8281 gacggatgga agctgtcaat gtcctaattg taacaaaccg ccctgccatt gctaactcat
     8341 ggtatgatga ttttgaaaca ttcatagcag gtcaaacgac ttacaagttt gtttctgaat
     8401 cagatagcct taagagtcgt ccaatcttgt cacgacaaga atttcttggt attttagctg
     8461 acgatgtaag acaacttgct tttatcagtc tccaagactt gaaaggatct gtttatttag
     8521 gtggagagca cgataaactc aaatgggtaa ctgatctgca ttgggacttg ttggttattg
     8581 acgaggctca tgaaggagtt gataccttca agactgacca agcctttaat aagattcgac
     8641 gaaattttac tctgcatttg tcaggtacat catttaaagc attggctaaa ggagatttta
     8701 cagaggaaca aatctacaac tggtcttatg ctgatgagca ggctgctaag tattcgtggt
     8761 ctcttgagca agaagaggaa aatccttatg aaagcttgcc tcagttgaat ctctttacct
     8821 atcaaatgtc tcagatgatt ggcgaaaagt tagaaaaagg cgctcagatc gatggtgaaa
     8881 atattgacta tgtttttgac ttaagtgaat ttttcgctac agatgataaa gggaaattta
     8941 ttcatgagca tgatgtcaga aattggttag atactctatc aagcaatgaa aaatatccat
     9001 tttcaaccaa agaactccgt aatgaactca agcatacttt ttggctttta gaacgtgtcg
     9061 cttcggccaa agcattaaaa gccctactag aagaacaccc aatctatgaa aactatgaga
     9121 tcgttctagc tgctggtgac ggacgtatgt ccgaagaaga cgataaagtc aaactcaaat
     9181 ccttggactt ggttagaaaa gcgatagcag agaatgacaa aaccattacc ctatccgttg
     9241 gtcagctgac gacaggtgtc actatccctg aatggacagg tgtattgatg ttatcaaatt
     9301 tgaaatcacc agctctttat atgcaggccg ccttccgtgc tcaaaatcct tactcatgga
     9361 gcgataacaa aggaaatcac tttcgcaaag aaagagccta tgtatttgac tttgcgccgg
     9421 aaagaacctt gattctcttt gatgagtttg ccaacaactt attgcttgta actgcagctg
     9481 gtagaggaac ttcagctaca cgcgaagaaa atattagaga attattaaac ttctttccaa
     9541 ttattgccga agaccgtgct ggtaagatgg ttgaaattga tgcaaaggca gttctaacca
     9601 ctcctcgcca gataaaagct agagaagttc ttaaacgagg ttttatgtcc aatctcttat
     9661 ttgataatat tagtggtatt ttccaagcaa gtcaaacagt tttagatatt ttaaatgagc
     9721 tgccagttga aaaggaaggg aaggtacaag atagttctga tttattagat ttttcagatg
     9781 ttacagtcga tgatgaggga aatgcagtag tagaccatga aattgtagtt aatcagcaaa
     9841 tgcgactttt tggtgaaaaa gtttatggac ttggtgaatc tgttgctgag ttagtcacaa
     9901 aagatgagga acgaactcaa aaacagctgg tcaatgactt gagtaagacc gtttcttcag
     9961 tgattgtaga ggaattgaaa gcagattatt ctctaaaaac aagggaaact gagcaaatta
    10021 agaaacaaat tacagcaaca cttgagaatg aaattcgaaa aaatgatatc gaaagaaaaa
    10081 tttctgaagc tcatatcaag caagagttgc aacagcagct caaagaagca aatgataaag
    10141 cgcaaaaaga taagattcaa gaagatttgg aaaaacgttt agaagaaaat aaactcattc
    10201 ataaagaaaa actagaacaa acactcaaaa aagaagtgga aaaaatgcct gagaaattta
    10261 tcgaacaggt tgagataaaa cgtgtggaac agttgaaaca atcagctcaa gatgaaattc
    10321 gtgaccattt acgagggttt gcaagaacaa ttccaagttt tattatggct tacggtgatc
    10381 aaactctaac acttgataat tttgatgcct ttgttcctga acatgttttt tatgaagtaa
    10441 cagggattac gattgatcag tttagatatt tgcgagatgg tgggcaggat tttgcagggc
    10501 atctctttga taaagcaaca tttgacgaag ctattcaaga atttcttcgc aagaaaaagg
    10561 agttggcgga ttattttaaa gatcaaaaag aagacatttt tgactatatt ccaccgcaga
    10621 agaccaacca aattttcact cctaaacgag tggtgaaaag gatggtagat gatttggaaa
    10681 aggaaaatcc agggattttt gatgatccat ctaagacttt tattgattta tatatgaagt
    10741 caggcctcta tattgcagaa cttgtgaagc ggttatataa tagcaatggc ttgaaagagg
    10801 cctttccaaa tcctgaagaa cgcttaaaac atattttgga aaagcaagtt tatggatttg
    10861 ctccgtctga gattatctat aacatttcca ctaattttat atttggcaat ctttctaaag
    10921 atatcagtag gaagaatttt gttttagcag ataccattcc agcggctaaa gaagggagca
    10981 ttcaaaagtt ggttgattcc tattttgaaa ataattaaaa agaaggccga gtcaaaattc
    11041 tttgaaatca gaaaaaacgc ataatattga gtgcttttgt actgcccccc aaaagttaga
    11101 cagaaaaaat ctaacttttg gggggcagtt cagacaatcc ttggtattat gcgttttatt
    11161 gtgggaagat gtataatgga ttgaaataag atatgaacaa atcaattagg aatttaaagc
    11221 attttataac aacgttttag agtaatgggg ggctatttca acttcaacct actataatac
    11281 agaaaaaaac aactccctga taattcaagg agttgtctat agttaaatta gtttttagaa
    11341 gcttcttgga attctgggtt tttccatgct tcgtcaatga tagcttgtaa ttctttagca
    11401 gatgcttgca ttttttgagt ttctgcgtcg ttcaatggga tatttactgg acgaacgata
    11461 ccatgtgcac caacaacagc tggttgaccg ataaagacat tctcaactcc gtattgacct
    11521 tcttggaata ctgaaagtgg aagtactgcg ttttcatcgt caaggattgc tttagtgata
    11581 cgagcaaggg ctactgcgat accgtagtat gttgcacctt ttttgttgat gattgtgtag
    11641 gctgcatcac gaacaccttc gaacaattca atcaattcag cttcttgaac attttgagtg
    11701 tctttaagga attcttcaag gtttacacca gcgatgttag cgtgtgacca aacagcgaac
    11761 tcagagtcac cgtgttcacc catgatgtag gcgtgcactg aacgagcatc cacatccaat
    11821 ttttcagcaa gtgcttgacg gaaacgagct gagtcaagtg aagtacctga accgataacg
    11881 cgttctttag ggaaaccaga gaatttccaa gttgagtaag tcaaaacgtc aactgggtta
    11941 gcagcaacaa ggaagatacc tttgaaacca gattcaacaa cttgagttac gattgatttg
    12001 ttgatagcaa ggtttttacc tacaaggtca agacgagttt cacctggttt ttgaggtgca
    12061 cctgcagtga tcacaacaag gtcagcgtct gcacagtcag agtattgagc tgcatagatt
    12121 tttttaggtg aagtgaaggc aagggcgtga ctaaggtcaa gcgcatcacc aacagctttt
    12181 tcatgcaatt gtggaatttc gataattcca agctcttgtg caattccttg gttaacaagt
    12241 gcaaaagcgt aagatgaacc tacagcacca tcaccgacaa ggataacttt tttgtgttgt
    12301 ttagttgaag tcattgtttt aaacatctcc ttaattttat taggggattt tccctagaca
    12361 acttcattct atcactttta aaaaactttg tcacgaatat gccttatagt tctcgatgta
    12421 aacgttttag tggtttagag gctgaaatag atgggaattt atggtataat gttgttactt
    12481 actaattgtg aaatgaggca tttattaatg caggataaaa atttagtgaa tgtcaatctg
    12541 acaaaggaga tgaaggcaag ttttatcgac tacgccatga gtgttatcgt agcgcgagct
    12601 cttcctgatg ttcgagatgg cttaaaacct gttcaccgtc gcattctcta cggaatgaat
    12661 gaattgggtg tgaccccaga caaaccccat aaaaaatctg ctcgtattac aggggatgtc
    12721 atgggtaaat atcacccaca cggggattcc tctatttatg aagccatggt ccgtatggct
    12781 caatggtgga gctaccgtta catgcttgta gatggtcatg ggaattttgg ttccatggat
    12841 ggagatagtg ctgccgctca acgttatacc gaggcacgta tgagcaagat tgctctggaa
    12901 atgcttcgtg atatcaacaa aaatacagtt gatttcgttg ataactatga tgccaatgaa
    12961 cgggaaccct tggtcttgcc agcgcgtttt ccaaaccttt tggttaatgg agcaactggt
    13021 atcgcggttg ggatggcaac caatattcca cctcataatc tgggtgaaac cattgatgca
    13081 gtgaagttgg tcatggataa tcctgaagtg actaccaagg acttgatgga agtcttgcct
    13141 ggaccagatt ttccaactgg tgctcttgtc atggggaaat caggtatcca taaggcttat
    13201 gaaacaggta aaggttcgat tgtcctacgt tctcgtacag agattgaaac gactaagact
    13261 ggtcgtgagc gtatcgttgt aacagaattt ccttacatgg tcaataaaac caaggtgcat
    13321 gagcatattg ttcgcttggt tcaggaaaaa cgcattgagg gtatcacagc agtacgtgat
    13381 gagtcaaacc gtgaaggtgt tcgatttgtt attgaagtca agcgcgacgc ctcagccaat
    13441 gttattctca ataacctctt caaaatgacc caaatgcaaa ccaattttgg tttcaatatg
    13501 ctcgctatcc aaaatggtat accgaaaatt ttgtctcttc gtcagatttt ggatgcttat
    13561 atcgagcacc aaaaagaagt ggttgttcgt cgtacacgtt ttgataagga aaaagcggaa
    13621 gcgcgcgctc atatcttaga aggtctcttg attgcgctag accatatcga cgaagtgatt
    13681 cgtatcatcc gtgctagtga aacggatgcg gaagctcaag ctgagttgat gagcaagttt
    13741 aagctttctg aacgtcaaag tcaagctatc cttgatatgc gtcttcgtcg tttgacaggt
    13801 ttggaacgcg ataagattca atctgagtat gatgacctct tggctctgat tgcggattta
    13861 gcagatattc ttgctaagcc tgaacgtgtt tctcaaatta tcaaagacga attggatgaa
    13921 gttaaacgta aattttctga taagcgccgt acagagttga tgattggaca ggtcttgagt
    13981 ctcgaggatg aggacttgat tgaagaatcg gatgtcttga ttaccctttc taacagaggc
    14041 tacattaagc gtttggatca ggacgagttc actgctcaaa aacgtggggg tcgtggtgtc
    14101 caaggaacgg gagtgaaaga tgatgacttt gttcgtgagt tagtgtcaac tagcacccat
    14161 gatcatctgc tcttcttcac aaacaaggga cgtgtctatc gtcttaaagg ttatgaaatt
    14221 cctgagtatg gtcggactgc caaagggcta ccagtagtca atctcttgaa attggatgaa
    14281 gacgaaagta ttcagacggt tatcaatgtt gagtctgatc gcagtgatga tgcttatctc
    14341 ttctttacaa cccgtcacgg tattgtgaag agaaccagtg ttaaggagtt tgccaatatt
    14401 cgtcaaaatg gtctcaaagc gctgaattta aaggatgaag atgagttaat caatgtcttg
    14461 ttggcagaag gagatatgga tattatcatt ggtaccaagt ttggttatgc agttcgcttt
    14521 aatcaatcag ccgttcgtgg tatgagccgt atcgccactg gtgtgaaagg tgttaacctt
    14581 cgtgaaggag acacagttgt tggtgccagc ttgattactg atcaagatga ggttcttatt
    14641 atcacagaaa aaggatatgg taagcgtaca gtcgctactg aatacccaac aaaaggtcgt
    14701 ggtggtaagg gaatgcagac agctaaaatt accgaaaaaa atggcttgct ggccggtctt
    14761 atgactgttc aaggggatga ggatttgatg attatcactg atacaggtgt catgattcga
    14821 accaatcttg ccaatatttc acaaacagga cgtgcaacta tgggagttaa agtaatgcgc
    14881 ctggatcaag atgctcagat agtgactttc acaacggttg cggtggcaga aaaagaagaa
    14941 gttgggacag aaaacgaaac agaaggtgaa gcataatgtc tcaaaaaaat aataaaaaga
    15001 aaaacaagcg aaaaaatctg ctgacaaata tcctagcagg atttctgata ttactgtcac
    15061 tggctttgat ttttaatact caaattcgaa atattttcat agtctggaat accaataagt
    15121 atcaagttag ccaggtatca aaagaaaaat tagaagaaaa tcaggataca gaaggcaatt
    15181 ttgactttga ttctgtcaaa gctatctctt cggaagctgt tctaacttct caatgggatg
    15241 ctcaaaaatt accagttatt gggggaattg caattcctga attggaaatg aatttgccga
    15301 tttttaaagg acttgataat gttaatctct tctacggagc tggtacaatg aaacgcgagc
    15361 aagtaatggg agaaggaaat tatagtctag ctagtcacca tatctttggt gttgataatg
    15421 ctaataaaat gttattttct cctttagata atgctaaaaa tggcatgaag atttatctaa
    15481 ccgataaaaa taaagtttat acttatgaaa tacgtgaagt caaacgtgtg acaccggatc
    15541 gtgttgatga agttgatgat agagatgggg tcaatgaaat cacattagta acctgtgaag
    15601 accttgctgc tacagaacgt attattgtca aaggtgattt gaaagaaaca aaagattatt
    15661 cacaaacatc taatgaaatc ctaacagctt tcaatcaacc atataaacaa ttttattaat
    15721 acaaatcagt gaaatccatg atttcactga ttttttaaga ttttcataaa aatatagtaa
    15781 aatgaaataa gaataggaca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
    15841 agaagtagag gtgtactatt ctagtttcaa tctactataa acttttatga aatacagaaa
    15901 acgcttccta aaacctaaag tttgtgatat aattatcaag aaaaaagatt taggagttta
    15961 ttatggtttc ttcggaattt atctcaaaga ttgaatttgc ttgcaataag aaagaaagtc
    16021 tttatagtca aagcaaattt aagtatgcga ttcgttcgat gttcgcaggt gcatttttaa
    16081 ccttcagtac tgctgcaggt gcagttgggg ctgacttgat taataaaatt gcaccaggta
    16141 gtggacgctt cctctttcca ttcgtttttg cttggggctt ggcctacatt gtttttttga
    16201 atgccgagtt ggtcacttca aacatgatgt tcttgactgc tggtagtttc ttaaaaaaaa
    16261 tctcttggag aaaaacagct gagattttac tatactgtac cttgttcaac cttatcggag
    16321 ccttgatagc agggtggggc tttgctcatt cggcagccta tgcgaatctg acacacgata
    16381 gtttcatctc aggtgttgtt gagatgaagt taggccgctc aaatgaattg gtcttgcttg
    16441 aggcgatttt ggcaaatatt tttgtaaata ttgcgattct gtcatttatt ttggtcaaag
    16501 atggtggtgc caaactttgg cttgtgttgt cagctattta catgtttgta ttcttaacaa
    16561 acgagcacat tgcggcgaac tttgcttctt tcgcgattgt gaaattcagt gttgctgcgg
    16621 attcaattgc caacttcggt gttggaaata tgcttcgcca ctggggtgtg actttcatcg
    16681 gaaactttat cggaggaggc ctcttgatgg gtcttccata tgccttcctc aataaaaacg
    16741 aagatactta tgtagattaa gaaaatgagc acgattgagt cgtgcttttt tcattttcaa
    16801 aataaggtaa tagctatttc ttatatcaaa atatagaaaa ctgatatttg taaactataa
    16861 ctcaaggtgc tacaatatcc ttaataaaat aatatggagg tcaccttatg acttgtgatt
    16921 ttaaatttga aactctacaa ctacatgctg gtcaagttgt ggctccagct actaagtctc
    16981 gtgcagtgcc gatttatcaa acaacatttt ttgtttttga tgacacgtag gaaggtgccg
    17041 atctgtttgc cttgaggaaa ccagggaaca tttatactcg tatcaccaat cctacaacag
    17101 ctgcccttga aggtggtgtt gaagcgctag caacagcatc aggtatgact gcagtgactt
    17161 atacgatttt ggcgattgcc catgctggtg accatgtagt ggctgcttcg actatttacg
    17221 gtggaacctt caatcttttg aaagaacccc ttcctcgtta tggtatcaca acaacctttt
    17281 tcgatattga taatttggag gaagtagaag cagctatcaa agacaatacc aagcttgtct
    17341 tgattgaaac cttgggtaac cccttgatta atattccaga cctggaaaaa ctggcagaga
    17401 ttgctcataa acatcaaatc ccacttgtgt cagacaatac ttttgcaaca ccttatttga
    17461 ttaacgtctt ctctcatggc gttgacattg ccattcactc tgtgactaag tttatcggtg
    17521 ggcatggtac aactattgga ggaataattg tcgatagtgg tcgttttgac tggacggctt
    17581 cagggaaatt ccctcaattt gttgacgagg gtccaagctg ccacaatttg agctatactc
    17641 gtgatgtggg tgcagcagcc tttattatag ctgttcgagt tcaattgctt cgtgatacag
    17701 gtgcagcctt gtcaccattc aatgctttcc tcttgctaca aagacttgaa acctcttcac
    17761 ttcgtgtgga acgccatgta caaaatgctg agacaattgt tgattttctt gtcaaccatc
    17821 ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag tccttatcat gccttggctg
    17881 agaaatactt gccaaaaggt gtcggttcaa tctttacctt ccacgtcaaa ggtggcgagg
    17941 aagaagcacg caaggtcatt gataatttag aaatcttttc tgaccttgca aacgcggcag
    18001 atgctaaatc gcttgttgtc catccagcaa caaccactca cggtcaattg tcagaaaaag
    18061 acctagaagc agcaggtgtc acaccaaact aaattcgttt gtcaatcggt cttgaaaatg
    18121 tagaagattt gattgaagac ttgcgcttgg ccttggaaaa aatttaaagt aaaagaagat
    18181 aaacagtggg cttcgactca ctgtttttga ttttccctca ggcatgatat aatggttaca
    18241 gaagtctaga aagaggaacg atatgaacga aatcaaatgt cccaactgtg gggaagtctt
    18301 tacagtaaat gagagtcagt atgccgaact cttgtcccaa gtgagaacgg cagagtttga
    18361 taaggaacta cacgatagga tgaagcagga actggccttg gctgagcaaa aggccatgaa
    18421 tgagcaacag actaaactgg ctcagaagga tcaagaaatt gcgcaattac agagtcagat
    18481 ccaaaacttt gatacagaaa aagaattggc caagaaagag gttgaacaga caagccatga
    18541 ggctctcttg gctaaggaca aggaagtaca gctcttagaa aatcagttgg ctaccttgcg
    18601 tttggagcat gaaaatcaac tacaaaagac cctttctgac ctagaaaaag aacgggatca
    18661 ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa ttatctttgg cttctgttaa
    18721 gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa gtcgagtttt ataagaattt
    18781 taaggctcaa caatctacaa aagcgattgg ggaaagccta gaacagtatg cagagagtga
    18841 gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac tttgagaagg ataacaaggt
    18901 ctcttcgcgt gggtctaaag gggactttat cttccgtgag tgtgatgaaa atggagttga
    18961 aatcatttct atcatgtttg agatgaaaaa cgaagcggac ggaacagaga agaagcacaa
    19021 gaatgcagat ttttacaagg aattggacaa ggaccgtcgg gagaagaact gtgagtatgc
    19081 cgttttggtg accatgcttg aggctgataa tgactacttt aacacaggga ttgttgacgt
    19141 cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa ttctttatcc aattgattgg
    19201 tctcttacgt aatgcggcgc taaattccct aaaatacaag caggagttgg ccttggttcg
    19261 cgagcaaaat attgacatta cgcattttga ggaagatttg gatgccttta agctagcttt
    19321 tgctaagaac tataattcag cttcgactaa ctttggaaaa gctattgatg aaatcgacaa
    19381 ggccatcaaa cgcatggaag aggttaagaa attcctgacc acatctgaaa accaactccg
    19441 tttagctaac aacaaattgg aagatgtctc tgttaaaaaa ttgacccgga aaaatccaac
    19501 aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa gcaaaaatga acggtattat
    19561 taacttaaaa aaggaagcag gaatgacctc gcatgatgcg gtttttaaac tgcgtaagat
    19621 tttgggaacc aagaaaattg gtcatggtgg aaccttggat ccggatgtgg tgggtgtttt
    19681 gccgattgcg gttggcaagg cgacacgcat ggtcgagttt atgcaggacg agggtaagat
    19741 ctatgagggg gaaatcactc tgggctattc cacgaagact gaggatgcta gtggggaagt
    19801 ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag cttgttgatg aagcgattgc
    19861 tagcttgact gggcctatta ctcagattcc ccctatgtat tcggcagtta aggttaatgg
    19921 tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg gagcgtccag aacgtcaggt
    19981 gaccatttat caatttgagc gaacaagtcc gatttcttat gatggccaac ttgcccgatt
    20041 cacttttcgt gtaaaatgca gtaaagggac gtacatccgt actttgtcag ttgatttggg
    20101 tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact cgtactagtg ctgctggctt
    20161 acaattagaa gacgctcttg ccttggagga aattgctgaa aaagtagagg ctgggcaatt
    20221 agattttctc catcctttag agattgggac aggtgacctt gtcaaagttt tcctaagtcc
    20281 agaagaggct acagaagttc gctttggtcg ttttattgag ctagaccaaa cggacaaaga
    20341 actggctgcc tttgaagatg ataaattgtt agccattcta gaaaaacggg gcaatctcta
    20401 taagccaagg aaggttttta gctagatcgt ttaggaataa aaatcgggtg atagataaca
    20461 attgcttgat aaaaccccat actaatagta gaatggtttt gggaattata atattccaat
    20521 tgttgcgagt tgtaggtact caaataatct atatagaaat ttagaggtgt gaaatgaagc
    20581 aatttaaaat tctttcagat aaatatttag agtccattac aggttctgat gggaacttag
    20641 gcccaggatt tggtgtgata attccatgat gcgaaatgag tttcgagaaa gggtggagca
    20701 acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg agtcacctgt ttcgtcttgc
    20761 tatacaaaat ttagacagaa atgaaaaata ccaatcggtc atggccaatt tgagtcaagg
    20821 gttgtcactt tacctcatga cgcatcatta ccaggcacct aagtctgtca ttgattttgg
    20881 tttatggatt gccaaagctc ctagccagga aagagggaga ctggctttct tgcagatgct
    20941 tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca tcagagggaa gactctggtg
    21001 ttttttgctg atgagactat aaattttgta aaaaatgtga tagaatagac gacggataaa
    21061 ataacggagg atagcatgca aaatagacca atcattattg gagtgacagg tggttctggt
    21121 ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt ttcctgatga aaagatttcc
    21181 atgattgagc atgattcata ctacaaggat cagtctcatt tgacctttga agagcgtgtc
    21241 aaaaccaact atgaccatcc ttttgccttt gatacagact tgatgatcga gcagattaag
    21301 gaattgttgg cagggcgtcc ggtggacatc ccgacctacg actatacaga gcatacacgg
    21361 agtagcaaga cctatcgtca ggaacctcaa gatgtcttta tcgttgaggg tattttggtc
    21421 ttggaggaca agcgtctgcg cgatttgatg gatatcaaga tttttgtgga tacggatgac
    21481 gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg agcgtggccg tagccttgat
    21541 agcgttatta accagtactt aggtgtggtc aaaccaatgt accaccagtt tatcgagtca
    21601 actaagcgtt atgctgatat cgtcattcct gaaggggtta gcaataccgt ggctatcgac
    21661 ctgttgacga ccaagattgc aaagattttg gaagaagctc gaaacagcaa ataatcacat
    21721 gtggaggcct tgcctccttt ttctattttt ccctcataat ggtacataaa tggagatttt
    21781 taggcatatt tttggtataa taatacccat gaaagagcaa gtaaatgaat agtaggtgga
    21841 gatggaaaag tatttatcgg taacaacttt gaccaagtat ctgaaaatga aattcgataa
    21901 agacccatac ttggaacggg tctatttaac tggtcaagtt tccaactttc gtaaacgacc
    21961 tactcaccaa tatttctccc taaaggatga ccatgcagtt attcaagcga ccatctggtc
    22021 tgggatttat cagaaattag ggtttgacct ggaagaagga atgaagatca atgtgattgg
    22081 gcgtgtacag gtctatgaac caagtggtag ctactccatc atcattgaaa aagctgagcc
    22141 tgatggggtt ggggcgcttg cgattcagtt tgaacaactt aagaaaaaat tgacagaaga
    22201 aggcctgttt caagaacgct tcaagcaagc tctgccccaa ttttctaaga gaattggtgt
    22261 agtaaccagc cgtagtggag ccgttattcg agatattatc acgaccgtca gcaggcgatt
    22321 cccaggtgtt gacatccttc tttatccgac caaggttcaa ggtgaagggg ctgcggagga
    22381 aattgctaga aatattgcgc gtgctaatca acgggacgat ttggatttgc tcattattgg
    22441 tcgtggaggt ggttctattg aggatctctg ggcctttaac gaagaaattg tggtacgagc
    22501 tatttttgaa tctcgtttgc cagttatctc tagtgtgggg catgagacgg atgtgacctt
    22561 ggcagatttt gtggcagatc gacgcgctgc aacgccaaca gcggcggctg aactggcaac
    22621 acctgtgacc aagttggatg tattagctca tttgcaaaat caggaaaaac ggatggtaac
    22681 ggcagtccga aatgttctat ctaagaaaca agaggctttg aaaaaatgca gtcagtctgt
    22741 tatctttaga caacctgagc gcttgtatga cggttatttg caacgcttgg accaactgca
    22801 actgcgtttg aaacaaagtt tgcgaactcg gatttctgat aacaaacaat tagttcaagc
    22861 aagaactcat caattagtac aattatcacc tgttaccaaa atccaacgct atcaagaccg
    22921 tttaggacag ttggacaagc tcttaggtag ccaaatggcg ttagtttatg acgccaaggt
    22981 tgctgaggcc aagcgacttt cggaagcttt gctcatgttg gatactagcc gaatcgtggc
    23041 gcgtggttat gctattgtca aaaaagaaga gtccgttgta gattcggttg agagtttgaa
    23101 gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa gtagaattag aggttaaaga
    23161 tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact ggaaaccatt gtccaaagtt
    23221 tggaaaatgg tgaaattgct ctggaagatg cgattactgc ctttcaaaag ggcatggtct
    23281 tgtcaaaaga gctccaagct acgctggaca aggctgaaaa gaccttggtc aaggtcatgc
    23341 aagaagacgg aacagaaagt gattttgaat gaaaaagcaa gaaaaattag ctcttgtcga
    23401 gtcggctttg gaagattttt atggagacca gcagtttgcc tctagtttac gggagtctgt
    23461 tctctattct attcatgctg gtggcaagcg tattcggcct tttctcttgt tagaagttct
    23521 ggaagccttg caggttacca tcaaacctgc tcacgcgcag gtagctactg ccttagagat
    23581 gattcataca gggagcttga ttcacgatga ccttcctgct atggatgatg acgattatcg
    23641 aagagggcgg ttaaccaatc acaagaaatt cggtgaagct atggccattt tggctggaga
    23701 tgccttattc ttagactcat atgccttgat tgcgcaggca gatttgccaa gtcagattaa
    23761 ggtggacttg attgccaact tatcccttgc ttcaggtagt ctgggtatgg tagcagggca
    23821 agttttggat atggagggcg aacaccagca cttgtctttg gaagaacttc agactattca
    23881 tgccaataag actgggaagt tactagccta tcccttccaa gcggcagcta ttatagctga
    23941 attgtcacct gaaatgcagg tgaagctgaa aactgtgggt gaattgattg gacttgcttt
    24001 tcaagtcaga gatgatgtac tggatgtgac agctagtttt gaggaaatcg gcaagacacc
    24061 tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc ctgttgggct tggaagagtc
    24121 cattgccttt tgtaaccaaa ccctggatca agctaatgaa aaattagaag aaattgccca
    24181 gcagcttccc tttgaaacag aatcgattgt aagtgtagta gaaagtttga gaatcaatgg
    24241 ctaaggaaag agtggatgta ctagcttata aacaggggtt gtttgaaacg agagagcagg
    24301 ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct taatggagaa cggtttgaca
    24361 agccaggaga gaaaattcca gatgacaccg aattaaaact caagggggag aaactcaagt
    24421 atgtcagccg tggtggtttg aaactggaaa aggccttgca ggtctttgat ttgtcggtgg
    24481 atggcgcgac tacgattgat atcggggcct ctactggagg ttttaccgat gtcatgctac
    24541 agaatagtgc caagttggtc tttgcagtcg atgttggtac caatcagttg gcttggaaat
    24601 tacgccaaga cccacgagtt gtcagcatgg agcagttcaa tttccgctat gctgaaaaga
    24661 ctgatttcga gcaggagccg agctttgcca gtattgatgt gagtttcatt tcccttagtc
    24721 tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg tcaggtggta gcacttgtca
    24781 aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa tggaattatt cgagatgcta
    24841 aggttcatca gaatgtcctt gaatctgtaa cagctatggc agtagaggta ggtttttcag
    24901 tccttggctt ggacttttct cccatccaag gtggacatgg aaatattgaa tttttagcgt
    24961 atttgaaaaa agaaaagtca gcaagcaatc agattcttgc tgagattaaa gaagcagtag
    25021 agagggcgca tagtcaattt aaaaatgaat aaaaaagaga gacttgaaaa aattagaaga
    25081 ttggtgacag attatcaaat cggcacgcaa gaagaaattg tagaacattt gaaagaagca
    25141 ggtatcactg ccactcaggc gacggtatcc cgagatatca aagagttagg tattgtcaaa
    25201 attcctttga gagacaacac ctatgtctac gaattgccaa aatcaatcgt aaaaagtctg
    25261 caactggctg aagacaatat cgaatcggct gaattgatgg ataagatgat caatctccaa
    25321 gttattccag gaaatacggc ttttgtaaaa gctcagttaa tcgagacttt tgcagacaag
    25381 atttttagct gtttgactga tgatagctcg attttagtca ttgccagaag tggaagtcta
    25441 gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct atatgttact tgaaatttcg
    25501 ataaaaaact ttgccattat tgaggctatt tccctcaatt ttgaaaaggg gatgactgtc
    25561 ctgactggtg aaacgggtgc agggaagtcg attatcattg atgccatgaa tatgatgttg
    25621 ggagctcgtg cgacgacaga tgttattcgt catggtgcac caaaggcaga gattgagggg
    25681 cttttctcag ttgagaatag tcgtctttta caggaaattt ttgatgagca aggtttagaa
    25741 ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa atggtcgtag tatcagccgt
    25801 gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta ttgggcaaca tcttgtagat
    25861 attcatggtc agcatgacca tgaggagtta atgcgtcccc aactgcatat ccagatgttg
    25921 gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa cctatcaaac gagttttgat
    25981 gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga aaaaccaaca ggaacacaag
    26041 gcacgtatcg aaatgttgga atttcaaatg gcagagattg aggcagcaaa cttgcaggct
    26101 ggagaagact tggctctcaa tcaagagcga gataaactcc tcaaccacaa aaatattgcg
    26161 gatacactga ccaatgccta cagtatgttg gacaatgaag atttttcaag tctggccaat
    26221 gttcgttcag ctatgaatga catggaaagt gtcgaagagt atgatcctga ataccgtgaa
    26281 atttcaagct atctgtctga gacctactat gttttagaag acattagcaa acgtttggaa
    26341 gctatcattg aggaccttga ttttgatggc aatcgcctga tgcaggttga gaatcgtttg
    26401 gacctccttc atactattac tcgtaagtat ggtgggactg ttgatgatgt tttgctttat
    26461 tttgccaaga ttacggaaga atacaatctc ttgacaggca ataatctttc gtctgaggac
    26521 atggaagcag agcttaagaa gttggaagtc aatcttgtca atttggcagg tcaacttgct
    26581 tctgctcgtc ataatttggc tcagcaactc gaagctgaga ttaaacaaga actgcaagat
    26641 ctttatatgg aaaaagccca gtttcaggtt cgttttagta agggaaaatt cagtcgtgag
    26701 ggaaataaaa tggttgagtt ttatatttca accaaccctg gagaagactt taaacccttg
    26761 gttaaggttg cttctggagg ggaattatct cgtctcatgt tagccattaa gtctgccttt
    26821 tcacgtaaag aaggcaagac tagcattgtc tttgatgagg tggatacggg agtttcaggt
    26881 cgtgttgctc aagctattgc tcagaaaatt cataaaattg gtcagcatgg tcaggttttg
    26941 gctatctccc atttgccaca agtaattgcg attgcagatt atcaattctt tattgagaag
    27001 attagtaatg accattcaac ggtttcgact gttcgtctct tgacggtcga agagcgagtg
    27061 gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag aagcagccct gacgcaagca
    27121 agagaattgt tgagaaacag ggagaaataa gatgacagat tattatgtaa ttggagatgt
    27181 tcacggaaaa gctgggatgc tggaagacct tctcaaaacc tgggatggtc agccccagtt
    27241 gctctttcta ggtgatttga ttgacagagg tgaggatagt caccgtgttc ttgaaatggt
    27301 caaggacttg gtggacaatc aaggggctat ctgtttgtcc ggaaaccacg agtatatgtt
    27361 tttgacttgg ctagatgacc cagaagaaag ttatgaccat tatagtcgca atggtggaga
    27421 tacaaccatt aactctatcc taggtcgtcc cttggatgca ccagttgatg gagtagaaga
    27481 tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa ttcattcgtc aaatgccatt
    27541 tgtagtagag acagacaagt atatctttgt tcacgcaggt attgatttga ccttggacga
    27601 ctggcatgaa acaacagatt ataaaaaagt atggcttaga aaaccattcc acggagctga
    27661 aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg gtctatggtt tgttacagca
    27721 agaacgcggt acatctgagc tttggactac agaagatggc aagattggaa tggatggagg
    27781 agctgtttat ggtggtgtcc ttcacgggat tgtctttaca gaccaaggaa tgacagaaca
    27841 ccactttatc gaaaatgacg gctttgtcgc tgaagattag tactcctagc agggtatggt
    27901 cttgtcaaaa tatcaaaaac aatttataat aaatagatac cctgaaagga agagaatcat
    27961 gaacttagaa gaattgaaaa aacgacagga gaagatccgt aacttctcta ttatcgccca
    28021 tattgaccac ggaaaatcaa ctctagcaga ccgcattttg gaaaaaacag agacggtttc
    28081 aagtcgtgaa atgcaggccc agcttttgga tagcatggag ctagagcggg aacgtggaat
    28141 tactattaag ttgaatgcca tcgagttgaa ttacactgca aaagatgggg aaacttatat
    28201 tttccacttg attgacacac cagggcacgt tgactttacc tatgaagttt cacgttcgct
    28261 agctgcctgt gagggtgcta ttttggtggt cgatgcggct caaggaattg aggctcaaac
    28321 tcttgccaac gtttatctgg ctttggataa tgatttggaa atcatgccta tcattaataa
    28381 aattgacctg ccggctgcag atccggagcg cgtgcgtaca gagattgaag atgtgattgg
    28441 tttggatgcc agtgaagcag ttttggcttc tgccaaggct ggtattggga ttgaagaaat
    28501 cctcgagcaa attgtagaaa aagtaccagc accaacgggt gatgtgacgg cgccacttaa
    28561 ggccttgatt ttcgactctg tttacgatgc ttaccgtggg gttatcctcc aagtgcgtgt
    28621 catggacgga gtggtcaaac ctggtgataa gattcagctc atgagcaata gtaagacctt
    28681 tgatgtggcc gaagtcggta tttttacacc aaaagcggtt ggtcgtgatt tccttgcgac
    28741 tggtgatgtt ggttacattg cggcgtctat caagacggtt caggatactc gtgtgggtga
    28801 taccgttacc ttggcaacca atcctgcggc agaaccatta catggttata agcagatgaa
    28861 tcctatggtc tttgcgggtc tctacccaat cgaatcaaac aagtacaatg acctgcgtga
    28921 agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag tttgaaccag aaacatctca
    28981 ggcacttgga tttggtttcc gttgtggatt tcttggactt ctccatatgg atgttatcca
    29041 ggaacgttta gagcgtgagt tcaatattga cctcatcatg acagctccgt ctgttattta
    29101 caaagttaat ttgaccgacg gtgagtctat ggatgtgtct aacccatctg agtttccaga
    29161 cccaactaag attgcgacca ttgaagagcc ttatgtcaag gcgcaaatca tggtaccaca
    29221 ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag cgtggggact ttgtgactat
    29281 ggactatatt gatgataacc gtgtcaatgt tatctatcaa attcctcttg ctgaaattgt
    29341 ctttgacttc tttgataaac ttaagtcttc gacacgtggt tatgcaagct ttgactacga
    29401 attgtcagaa tatcgcccat ctaagctggt gaaaatggat attcttctca atggagataa
    29461 ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc tacgaacgtg ggaaactcat
    29521 cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt gaagttccaa tccaagcggc
    29581 tattggacac aagattgtcg ctcgtactga tatcaaggcc cttcgtaaga acgtacttgc
    29641 taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc cttgaaaaac aaaaagctgg
    29701 taagaaacgc atgaaatcca tcggatcagt tgaagttccg caagaagcct tcctcagcgt
    29761 cttgagtatg gatgaagaat aagttatcag aaatgccttt ccttgttcac aggaaattta
    29821 tataagaatc aatagtaaag caggcttaga tggtctgctt ttttagtttt tatactcttc
    29881 gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat gtatggttac tgacttcgtc
    29941 agtttcatct acaacctcaa aaccatgttt tgagctgact tcgtcagttt catctacaac
    30001 ctcaaaactg tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat
    30061 tgagtattag aacagaaaaa tgcttggagt atttgtttgt gtgtttattt ttatataaca
    30121 aactataaac aaaacaaaaa tataaaaaaa gagacaaaaa agaatagaaa gtaattaaca
    30181 acgatttcat atagtgttat acttatagca taaagttaat ggaaagaagg gaaaacctta
    30241 tggtattaga ttatttcttt gacaaaaacc ttgtgttttg cttagaagcg gataatcaag
    30301 aataactttt tgatcaggta gcaagcttgt tggaagaacg agaaattgtc actccaactt
    30361 atcgtgaagc cttgatcacg cgtgaaaagt catttccaac tggtttatat atggaatttc
    30421 taggaaagga cttgcaaatg taatgattcc tcacacagat attgttcata atctagctga
    30481 gaaagtggtg gttgttcgat tagagaaacc ggtgactttc cataatatga ttgcgccagg
    30541 taaggaagta gaagtatcct tgctcttctt tatcatcaat aattcaagtt caagtcaaac
    30601 gaacattctt gctcagttaa tggacttctt cacaggaaat ggtcatcttg aagacctatc
    30661 aaaaatttct gaaccagaag ccctttatgc ttacattgct gaagcaaccg cttaatcttg
    30721 tctattaaaa aataaaatcg gaggaaatcc aaatgattaa aattcttgct gcctgcggtg
    30781 caggtgttaa ctcaagtcac caaattaaaa gtgctctaga agaagaactt tcaaaccgtg
    30841 gttacgatgt tcactgtgat gcagttatgg tgaaagatgt aaacgaagac cttatgaaag
    30901 gttacgatat ctttacacca atcgctgcaa cagaccttgg ttttgaacca ggtatcccag
    30961 ttatcgaagc tgggccaatc ttattccgta tcccagcaat gagcgttcca gtatttgaca
    31021 atattagact tcctgcgaaa caaaacatgg tatagtagtt ctatgaatga tgaagcaagt
    31081 aaacaactaa ctgatacacg atttaaatgt cttgttggtg ttcagcgcac gacttttgaa
    31141 gagatgttag ctgtgttaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa
    31201 cctaaattaa gcctagaaga ccttcttatg gccactcttc actatgtgcg agaataccgc
    31261 acttatgaag aaattgcggc tgattttggt atccacgaaa gcaacttact ccgtcggagc
    31321 caatgggttg aagtaactct tgttcaaagg tggtgttacg atttcaagaa ctcctctcag
    31381 ttctgaagac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgac ctaaaaaaag
    31441 aattagcgaa ttattctggt aaaaagaaat
//
LOCUS       DBDESG010000022        31043 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.022, whole genome shotgun sequence.
ACCESSION   DBDESG010000022 DBDESG010000000
VERSION     DBDESG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 31043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31043
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.022"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            17..289
                     /locus_tag="ACKB38_000960"
     CDS             17..289
                     /locus_tag="ACKB38_000960"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGL7979745.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKYHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            483..1817
                     /gene="trmFO"
                     /locus_tag="ACKB38_000961"
     CDS             483..1817
                     /gene="trmFO"
                     /locus_tag="ACKB38_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGL7979746.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGAGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLSSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1901..2638
                     /gene="pyrH"
                     /locus_tag="ACKB38_000962"
     CDS             1901..2638
                     /gene="pyrH"
                     /locus_tag="ACKB38_000962"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGL7979747.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2647..3204
                     /gene="frr"
                     /locus_tag="ACKB38_000963"
     CDS             2647..3204
                     /gene="frr"
                     /locus_tag="ACKB38_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGL7979748.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDIGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKREKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3264..4118
                     /gene="cvfB"
                     /locus_tag="ACKB38_000964"
     CDS             3264..4118
                     /gene="cvfB"
                     /locus_tag="ACKB38_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGL7979749.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4127..4342
                     /locus_tag="ACKB38_000965"
     CDS             4127..4342
                     /locus_tag="ACKB38_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGL7979750.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4428..5396
                     /locus_tag="ACKB38_000966"
     CDS             4428..5396
                     /locus_tag="ACKB38_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGL7979751.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5465..6279
                     /locus_tag="ACKB38_000967"
                     /pseudo
     CDS             5465..6279
                     /locus_tag="ACKB38_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
     gene            6462..6962
                     /locus_tag="ACKB38_000968"
     CDS             6462..6962
                     /locus_tag="ACKB38_000968"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7979752.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6965..7291
                     /locus_tag="ACKB38_000969"
                     /pseudo
     CDS             6965..7291
                     /locus_tag="ACKB38_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7592..8691)
                     /gene="ald"
                     /locus_tag="ACKB38_000970"
                     /pseudo
     CDS             complement(7592..8691)
                     /gene="ald"
                     /locus_tag="ACKB38_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8868..9437
                     /locus_tag="ACKB38_000971"
     CDS             8868..9437
                     /locus_tag="ACKB38_000971"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7979753.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9505..10155
                     /locus_tag="ACKB38_000972"
     CDS             9505..10155
                     /locus_tag="ACKB38_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGL7979754.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10139..12379
                     /locus_tag="ACKB38_000973"
     CDS             10139..12379
                     /locus_tag="ACKB38_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGL7979755.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLL
                     GYLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12558..12746
                     /locus_tag="ACKB38_000974"
     CDS             12558..12746
                     /locus_tag="ACKB38_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979756.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            12779..13366
                     /locus_tag="ACKB38_000975"
     CDS             12779..13366
                     /locus_tag="ACKB38_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7979757.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            13370..14554
                     /locus_tag="ACKB38_000976"
     CDS             13370..14554
                     /locus_tag="ACKB38_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979758.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    14718..14849
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14865..15395
                     /gene="infC"
                     /locus_tag="ACKB38_000977"
     CDS             14865..15395
                     /gene="infC"
                     /locus_tag="ACKB38_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGL7979759.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15428..15628
                     /gene="rpmI"
                     /locus_tag="ACKB38_000978"
     CDS             15428..15628
                     /gene="rpmI"
                     /locus_tag="ACKB38_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGL7979760.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15680..16039
                     /gene="rplT"
                     /locus_tag="ACKB38_000979"
     CDS             15680..16039
                     /gene="rplT"
                     /locus_tag="ACKB38_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGL7979761.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16097..16477
                     /locus_tag="ACKB38_000980"
     CDS             16097..16477
                     /locus_tag="ACKB38_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGL7979762.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16727..17527
                     /locus_tag="ACKB38_000981"
     CDS             16727..17527
                     /locus_tag="ACKB38_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGL7979763.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIDGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            17538..18476
                     /locus_tag="ACKB38_000982"
     CDS             17538..18476
                     /locus_tag="ACKB38_000982"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGL7979764.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18518..20551
                     /locus_tag="ACKB38_000983"
     CDS             18518..20551
                     /locus_tag="ACKB38_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGL7979765.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20612..22267)
                     /gene="pavA"
                     /locus_tag="ACKB38_000984"
     CDS             complement(20612..22267)
                     /gene="pavA"
                     /locus_tag="ACKB38_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGL7979766.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22372..22869
                     /gene="ybeY"
                     /locus_tag="ACKB38_000985"
     CDS             22372..22869
                     /gene="ybeY"
                     /locus_tag="ACKB38_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGL7979767.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRNTDRPTDVISLEYKPELEIAFDEEDLLENSELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22850..23245
                     /locus_tag="ACKB38_000986"
     CDS             22850..23245
                     /locus_tag="ACKB38_000986"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGL7979768.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23262..24161
                     /gene="era"
                     /locus_tag="ACKB38_000987"
     CDS             23262..24161
                     /gene="era"
                     /locus_tag="ACKB38_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGL7979769.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24210..25034
                     /gene="mutM"
                     /locus_tag="ACKB38_000988"
     CDS             24210..25034
                     /gene="mutM"
                     /locus_tag="ACKB38_000988"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGL7979770.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDVYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            25034..25639
                     /gene="coaE"
                     /locus_tag="ACKB38_000989"
     CDS             25034..25639
                     /gene="coaE"
                     /locus_tag="ACKB38_000989"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGL7979771.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25626..26825
                     /locus_tag="ACKB38_000990"
     CDS             25626..26825
                     /locus_tag="ACKB38_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HGL7979772.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26825..26974
                     /gene="rpmG"
                     /locus_tag="ACKB38_000991"
     CDS             26825..26974
                     /gene="rpmG"
                     /locus_tag="ACKB38_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGL7979773.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            27014..27247
                     /gene="secG"
                     /locus_tag="ACKB38_000992"
     CDS             27014..27247
                     /gene="secG"
                     /locus_tag="ACKB38_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGL7979774.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            27349..29703
                     /gene="rnr"
                     /locus_tag="ACKB38_000993"
     CDS             27349..29703
                     /gene="rnr"
                     /locus_tag="ACKB38_000993"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGL7979775.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLEFGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRDSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29666..30133
                     /gene="smpB"
                     /locus_tag="ACKB38_000994"
     CDS             29666..30133
                     /gene="smpB"
                     /locus_tag="ACKB38_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGL7979776.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQEIKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            30149..31009
                     /gene="tehB"
                     /locus_tag="ACKB38_000995"
     CDS             30149..31009
                     /gene="tehB"
                     /locus_tag="ACKB38_000995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGL7979777.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
BASE COUNT         9227 a         5771 c         7071 g         8974 t
ORIGIN      
        1 atcgtgcatc agttagttgt ttacttgctt catcattcat agaactacta taccatattt
       61 tgtttcgcag gaagtctatt ggaaagtaag aaatattgaa gctgaggcta ttagaagaaa
      121 ttgtgagcgt ggtgctattt tttcaggtaa aataaaatat cacgaagatt cacagtttaa
      181 aggagatcac tatgttgaat gttatgctgt tttagataat acggttatag caagagatag
      241 aataacagtc cctatcgatc cgttatgtgg aaaagatttt atagagtagc atataattga
      301 ttcttaactg gaatactcac tatctcttta catcaagaaa atgactaaac agggaagttt
      361 gccttcttcc ctttttttgt tatactagta gaagaaaaaa ttagaaagat ttgtgggtgt
      421 caaacagccc agtggggtgt tttaatatgg acttaggtcc cacccaaaga ggtattagtg
      481 tcgtgtctca atcttatatc aatgttatcg gtgctggttt ggcaggttct gaagcagctt
      541 accaaatcgc agagcgtggt attccagtta aactatatga aatgcgtggt gtcaagtcta
      601 caccccagca taaaacagac aattttgctg agttggtttg ttccaattct ttgcgtgggg
      661 atgctttgac aaatgcagtt ggtcttctca aggaagaaat gcgtcgcttg ggttctgtta
      721 tcttggaatc tgctgaggct acacgtgttc ctgcaggtgg tgcccttgca gtggaccgtg
      781 atggtttctc tcaaatggtg accgaaaaag ttgccaacca ccccttgatt gaagtggttc
      841 gtgatgaaat tacagaattg ccgacagatg ttattacggt tatcgctact ggtcctttga
      901 caagtgatgc cttggctgaa aagattcatg ctcttaatga cggtgctggt ttttatttct
      961 acgatgcggc agcgcctatt atcgatgtca acactatcga tatgagcaag gtctacctca
     1021 aatcacgtta tgataaggga gaagcggcct acctcaatgc ccctatgacc aagcaagaat
     1081 ttatggattt ccatgaagct ttggtcaatg cagaagaagc accgcttagt tcttttgaaa
     1141 aagaaaagta ctttgaagga tgtatgccta tcgaagtcat ggccaaacgt ggcattaaaa
     1201 ctatgcttta tggccctatg aagccagtcg gtcttgagta cccagacgac tatacaggac
     1261 ctcgtgatgg agaatttaaa acaccttatg cggttgtgca acttcgtcag gataatgcag
     1321 ctggtagcct ctacaatatt gttggtttcc agacccacct caaatgggga gaacaaaagc
     1381 gtgtcttcca aatgattccg ggtcttgaaa atgcggagtt tgtccgttat ggtgtgatgc
     1441 atcgcaattc ttacatggat tcaccaaatc ttcttgagca gacttaccgt tctaagaaac
     1501 aaccaaatct cttctttgct ggtcaaatga cgggtgtgga aggctatgtt gagtcggcgg
     1561 cttcaggctt agttgcggga attaacgcag ctcgtctctt caaggaagaa agcgaggcta
     1621 ttttccccga gacgacagcg attggaagct tagctcatta cattacccat gccgacagca
     1681 aacatttcca accaatgaat gtcaattttg ggatcatcaa ggagttggaa ggcgagcgta
     1741 tccgtgataa gaaggctcgt tatgaaaaaa ttgcagagcg tgcccttgcc gacttagagg
     1801 aatttttgac tgtctaattt ttttgaaaga attgctcatg atactataaa aatcttagaa
     1861 attgtgataa aataggtagg atgaaagaag gagagtgaaa atggcgaatc ccaagtataa
     1921 acgtatttta atcaagttat caggtgaagc ccttgccggt gaacgtggcg tagggattga
     1981 tatccaaaca gttcaaacaa tcgcaaaaga gattcaagaa gttcatagct taggtatcga
     2041 aattgccctt gttatcggtg gaggaaatct ctggcgtgga gaacctgcag cagaagcagg
     2101 tatggaccgt gttcaggcag attacacagg aatgcttggg actgttatga atgctcttgt
     2161 gatggcagat tcattgcaac aagttggggt tgatacgcgt gtacaaacag ctattgccat
     2221 gcaacaagtg gcagagcctt atgtccgtgg acgtgccctt cgtcaccttg aaaaaggccg
     2281 tatcgttatc tttggtgctg gaattggttc accttacttc tcgacagata caacagcggc
     2341 ccttcgtgca gctgaaatcg aagcagatgc catcctcatg gctaaaaatg gtgtcgatgg
     2401 tgtttacaat gccgatccta agaaagataa gacagctgtt aagtttgaag aattgaccca
     2461 ccgtgacgtt atcaataaag gtcttcgtat catggactca acagcttcaa ccctctcaat
     2521 ggacaacgac attgacttgg ttgtattcaa catgaaccaa ccaggcaaca tcaaacgtgt
     2581 cgtatttggt gaaaatatcg gaacaacagt ttcaaataat atcgaagaaa aggaataaga
     2641 aagaatatgg ctaacgcaat tattgaaaaa gctaaagaga gaatgaccca gtctcaccaa
     2701 tcacttgctc gtgaatttgg tggtatccgt gctggtcgtg ccaatgcaag cttgcttgac
     2761 cgtgtacatg tagaatacta tggagtcgaa actcctctta accaaatcgc ttcaattacg
     2821 attccagaag cgcgtgtttt gttggtaaca ccatttgaca agtcttcatt gaaagacatc
     2881 gaacgtgcct tgaacgcttc tgatattggt atcacaccgg ctaatgacgg ttctgtgatt
     2941 cgcttggtta tcccagctct tacagaagaa actcgtcgtg accttgctaa agaagtgaag
     3001 aaggtcggcg aaaatgctaa agtggctgtc cgcaatatcc gtcgcgatgc tatggacgaa
     3061 gctaagaaac gagaaaaagc aaaagaaatc actgaagacg aattgaagac tcttgaaaaa
     3121 gacattcaaa aagtaacaga cgatgctgtt aaacacatcg acgacatgac tgctaacaaa
     3181 gagaaagaac ttttggaagt ctaaaaataa acagaaaaac tcagttggca ttgctggctg
     3241 agttttattc gaaagaagga aatatgaata caaatcttgc aagttttatc gttggactga
     3301 tcatcgatga aaacgaccgt ttttactttg tgcaaaagga tggtcaaacc tatgctcttg
     3361 ctaaggaaga aggccaacat acagtagggg atacggtcaa aggttttgca tacacggata
     3421 tgaagcaaaa actccgcctg acaaccttag aagtgactgc cactcaggac caatttggtt
     3481 ggggacgtgt cacagaggtt cgtaaggact tgggtgtctt tgtggataca ggccttcctg
     3541 acaaggaaat cgttgtgtca ctcgatattc tccctgagct caaggaactc tggcctaaga
     3601 agggcgacca actctacatc cgtcttgaag tggataagaa ggaccgtatc tggggcctct
     3661 tggcttatca agaagacttc caacgtcttg ctcgtcctgc ctacaacaac atgcagaacc
     3721 aaaactggcc agccattgtt taccgtctca agctgtcagg aacttttgtt tacctaccag
     3781 aaaataatat gcttggtttt attcatccta gcgagcgtta cgcagagcca cgtttggggc
     3841 aagtattaga tgcgcgcgtt attggtttcc gtgaagtgga ccgcactctg aacctctccc
     3901 tcaaaccacg ctcctttgaa atgttggaaa acgatgctca gatgattttg acttatttgg
     3961 aaagcaatgg cggtttcatg accttaaatg acaagtcatc tccagacgac atcaaggcaa
     4021 cctttggcat ttctaaaggt cagttcaaga aagctttagg tggtcttatg aaggctggta
     4081 aaatcaagca ggaccagttt gggacagagt tgatttaggg aggcttatga gaaaatcatt
     4141 ttacacttgg ctcatgaccg agcgcaatcc taaaagtaac agtcccaaag caattttggc
     4201 agacctcgct tttgaagagt cagcctttcc aaaacacaca gatgattttg atgaggtcag
     4261 tcgctttttg gaggagcatg ccagtttctc ttttaaccta ggagattttg acagcatttg
     4321 gcaggaatat ctagaacact agcatttatt cattgggttt gggctagtaa tttctccatc
     4381 cctctgctat aataaaaaga aataaaagga ttagagaggt tctttatttg aaggaacatt
     4441 caatagacat tcaactgagt catccagatg acctgtttca tctttttggt tccaatgaac
     4501 gccatcttcg tttgatggaa gaagagcttg atgttgtgat tcatgctcgt acggagattg
     4561 tccaggtttt gggagaagag tctgcctgtg aggaagcccg tcaagttatt caggctttga
     4621 tggtcttggt aaatcgtggg atgaccgttg gtacgccaga tgtagtcact gcgattagca
     4681 tggtcaaaaa tgatgaaatt gacaagtttg tcgcccttta cgaagaagaa attatcaagg
     4741 ataatactgg gaaacctatc cgtgtcaaaa ccctagggca aaagctttat gtggacagtg
     4801 tcaaacagca tgatgtgacc tttggaattg ggccagcagg tacagggaag accttccttg
     4861 cagtgacctt ggcagtgact gcccttaaac gtgggcaagt caagcgaatt atcctaactc
     4921 gtccagcggt ggaagcggga gagagtcttg gatttcttcc gggtgatctt aaggagaagg
     4981 tggatcctta ccttcgtcct gtttacgatg ccttgtatca aattcttggg aaagaccaaa
     5041 cgactcgtct catggagcgt gaaattatcg aaattgcgcc ccttgcctat atgcgtggcc
     5101 ggaccttgga tgatgccttt gtcattctcg atgaggcgca aaacacgacc atcatgcaga
     5161 tgaagatgtt cttgacgcgt ttaggttttc attctaagat gattgtcaat ggagatatta
     5221 gtcagattga cctgccacgt aatgtcaagt ccggtttgat tgatgctcaa gagaaactca
     5281 agaacatcca tcagattgac tttgttcatt tttcagccaa ggatgtggtt cgccatcctg
     5341 ttgtcgctca gattatccga gcctatgaat attctactga agttgcacac gactgatttt
     5401 gaggaagttc gcctgcaaaa gaatagactt gttcggtaac tgtaaaaagt gttatactat
     5461 ttttatggaa acagtatacg acaaagcaca aaaacttaac tcaaaaaact tcaaactatt
     5521 gattggtgtc aaaaaggaaa cctttcaact catgctagaa cacctgaatt cagcctatca
     5581 gattcagcac cgaaaaggtg gacgtccacg tagtctgccc atggaagacc agctcattat
     5641 gaccctccgt tacttgcgat attatcccac tcagcgtctg ctggcctttg attttggcgt
     5701 cggtgtagct acggtaaatg ccatcatcac ttgggtggag gatacacttc gtgcgtcagg
     5761 tagctttgat ttggaccatt tagaagcccc gagtgctgct gtggctattg acgtgaccga
     5821 aagtccgatt cagcgtccaa acaaaaccaa agcaaaaatt attctggtaa aaagaaacga
     5881 cacaccttaa aaactcaaat tatgctggat ttgacgacac ataaagtctg tcaaatggcc
     5941 ttttctgacg gacatacgca tgattttact ctcttcaaag aaagtattgg acaaagtttg
     6001 cctgaaacga cgcttgcctt tgttgaccta ggttatttag gcatcttgaa atttcatgag
     6061 aatactttca ttcctgctaa aaattccaaa aatcgccgcc tgagtgagga tgataagcag
     6121 ttaaataaag agatgtcagc gatacgaatt gaaattgaac attttaacgc taaattcaag
     6181 accttccaaa tcatgtcagt cccttatcgt aaccgcagaa aacgtttcga gttacgggcg
     6241 gaattaattt gtgccatcat caattatgaa gtgaactaga ttccgaacaa gtctaatata
     6301 cttttgagag aggaaaatcc agttgtatag gctaaaggtt ttatccaaag gtctgagaca
     6361 acgattaggc acgatggaaa gaacttttat gtggctgatg acgatcagtg catcttcctg
     6421 tgtcataatc acagggcaca agaaagtagg aatttgaaaa gatgattgac caactatcta
     6481 agtattacag ttgtaggata ctaactgaaa aggatattcc aagtatttta tctttatatg
     6541 aaagtaatcc tctgtatttt cagcattgtc caccagagcc aaattttgca actgtaaaag
     6601 aggacatgct ttgtctacct gaaggtaaag ctaaggctga taagtttttt gttggatttt
     6661 ggaatggatc tgaccttgtg gctgttatgg attttgtcta tgcatatcct gatgaggaga
     6721 ctgtttttat tggtttgttt atggttgatc aagcctatca gagaaaaggg attggtagtc
     6781 atattgtgac agaagcacta gcttattttg ctaagaactt tcgaaaggca cgtttggctt
     6841 atgttaaggg aaatccgcaa tctcagcatt tttgggaaaa gcagggcttt aaatcaattg
     6901 gatgcgaggt taagcaagaa ctctatacgg ttgttatcgc tgaacagagc ctagaagatt
     6961 agaaatggca tcaagtaaga actatttgga atttgttttg gaacaattat caggattaga
     7021 tgatgtgact taccgttcca tgatggggga gtatattctt tacttccgcg gcaagattat
     7081 tggcggcatt tatgacgatc gctttttagt taaacccgtg caagcagtct tagataagat
     7141 tgaccaatct tcttttgagt ttccatacaa aggtgccaaa gaaatgattt gagtggaaga
     7201 acttgataat aagatgtttc tataagacct aattttagct atgtataacc aactgccaac
     7261 gcccaaacct aaaaagaaaa agcaagggtg aacgaagtaa aaaagaagtc tgctaaggcc
     7321 ctgtctttgc acgggtaaaa ttttatatat aaaaagaagc tgggactaaa gagctcagct
     7381 tcctttggtt tatataattg tcattacaag acgaagtggt tgggcgaaac tctgttgact
     7441 ttattcaatt tagagtttct tatgcacaat tgagtctgga acgaaagtct ccagttgcaa
     7501 agtatacagt acaataaacc aacgatgtaa tagctgatga cacaaagcac agtgggtagg
     7561 acttgcgaag tcaccctttt cttttcaaaa tttatactaa atcattgata tcagtgtagt
     7621 cacgattaag tccttgagca actggtaggt tagtcaagta accttgataa gtagtcacac
     7681 cttgacgcaa gccttcatct tcagagattg cttgtgcgaa tcctttgcca gccaaagctt
     7741 cgatataagg aagagtgaca ttggttaggg cgatggttga agtgcgagca accgcaccag
     7801 ggatattggc aacggcatag tggagaacac cgtgtttttc atagacgggt tcatcgtgcg
     7861 ttgtcacacg gtcagctgtt tcgataacgc caccttggtc aacagcaacg tcaacgatac
     7921 agagcctgga cgcatttgtt tgaccatctc atctgtcacc aattccggtg cttttgcacc
     7981 agggatgaga atggctccaa tcaccacatc agcatctctc acacttgctt caatgttgaa
     8041 tgaattagac ataagagttt gaatttgact tccaaagact tcttctagaa ctgagagacg
     8101 cttggaacta atatctaaaa tagtcacttg agcaccaaga ccaagggcga tgcgggcagc
     8161 atgtgtaccg acgacaccac caccgatgat agttactttt ccttttggaa cacctggtac
     8221 accaccaagt agaacaccag agccaccagc ttgcttagta aggaagtgag ctccgatttg
     8281 aacagccata cgacctgcaa cctcactcat aggaacgagg agcggtagtt gtccttgatt
     8341 gtcacgaaca gtttcagttg tttttgctgt taacatagca tctgctaatt ctggagcagc
     8401 ggccatgtgc aagtaggtga agagaagaag atcgtcgcgc aagtaaccgt attcagaact
     8461 taaagattct tttactttca caaccaactc tgctgcccaa gcttcaccag cagtagcgac
     8521 aatctcagct ccttgctttt gatagtcagc atcagtaaag ccagaaccga gaccagcatt
     8581 tgtttcgata aggacacgat gaccacgact aactaagcta tgaacacctg caggtgtgag
     8641 ggcgacacgg ttttcgttat ttttaatttc ttttgggatt ccgattaaca ttgagataac
     8701 ctacctttca attgacggtc ttgttttggt tgtcacattc cagttcataa atcaaaaatg
     8761 tgacggtttc attgtatatg aaaccgcttc aaaaatcaag aaaaacttgt catccaaatt
     8821 tttttatgct agactagtga aaatcaagct ctaatggagg gaaaagtatg gaatcaatat
     8881 ttgtgaaatt tgcccagtat ccgtctatag aaacggagcg tttattgctc agacctgtaa
     8941 ctttggatga tgcggaagca atgtttgact atgcctcgga caagggtaat acacgttaca
     9001 cttttccaac caatcaaagc ttggaagaaa ccaagaataa cattgctcag ttctacttgg
     9061 ctaatccctt gggacgttgg ggaatagaac taaaaagcaa tggtcagttt attggaacca
     9121 ttgacttgca caagattgat tctgttctta agaaggcagc tattggctac attatcaata
     9181 aaaagtattg gaatcaagga ttaacgacag aagccaatcg tgctgtgatt gagctagctt
     9241 ttgagaagat agggatgaat aagttgactg cccttcacga taaggctaat cccgcgtcag
     9301 gaaaggtcat ggagaaatca ggcatgcgtt tttcccatgc agaaccatat gcttgtatgg
     9361 accagcatga aaaaggccga atcgtgacaa gagttcatta tgtcttgacc aaggaagact
     9421 attttgcaaa taaataagca gttgaaaaga aatttttcga ctgttttttc ttcctcttac
     9481 gaataatcta agagaggaga aaatatggaa gcaattatcg agaaaatcaa agagtataaa
     9541 atcatcgtca tctgtactgg tctgggcttg cttgtaggag gatttttcct gctaaaacca
     9601 gctccacaaa cacctgtcaa agagacgaat ttgcaggctg aagttgcagc tgtttccaag
     9661 gactcatcga ccgaaaagga agtgaagaag gaagaaaagg aagaacccct tgaacaagat
     9721 ctaatcacag tagatgtcaa aggtgctgtc aaatcgccag ggatttatga cttgcctgta
     9781 ggtagtcgag tcaatgatgc tgttcagaag gctggtggct tgacagagca agcagacagc
     9841 aagtcgctca atctagctca gaaagttagt gatgaggctc tggtttacgt tcctactaag
     9901 ggagaagaag cagttagtca acagactggt tcggggacag cttcttcaac aagcaaggaa
     9961 aagaaggtca atctcaacaa ggccagtctg gaagaactca agcaggtcaa gggactggga
    10021 ggaaaacgag ctcaggacat tattgaccat cgtgaggcaa atggcaagtt caagtcagta
    10081 gacgagctca agaaggtctc tggcattggt ggcaaaacaa tagaaaagct taaagactat
    10141 gttacagtgg attaagaatt tctctattcc cctaatttac ctgagttttc tattactttg
    10201 gctttattac gctattttct cagcatctta tcttgctttg ttgggctttg tttttctgct
    10261 agtctgtctc tttatccaat ttccgtggaa atctgctggt aaagttctaa taatttgcgg
    10321 aatctttgga ttttggtttg tttttcaaaa ttggcaacag agtcaagcga gtcaaaatct
    10381 ggcggattct gttgaaaggg tacggatttt gcctgatact attaaggtta atggtgatag
    10441 tctatccttt cgtggcaagt ctaacggtcg tgctttccaa gtctattata aactccagtc
    10501 cgaggaggag aaagaagcct ttcaagcttt aactgacctg catgagatag gactagaagg
    10561 gaagctttcg gagccagaag ggcagagaaa ttttggtggc tttaattacc aagcctatct
    10621 gaagactcag ggaatttacc agactctcaa tatcaaaaca atccagtcac ttcaaaagat
    10681 tggcagttgg gatataggag aaaacttgtc cagtttacgt cgaaaggctg tggtttggat
    10741 taagacgcac tttccagacc ctatgggcaa ttacatgaca ggactcttgc tgggatatct
    10801 ggacaccgac tttgaggaga tgaatgagct ttattccagt ctaggaatta tccacctctt
    10861 tgccctatct ggcatgcagg taggtttttt catgaatgga tttaagaaac ttctcttgcg
    10921 attgggcttg acccaagaaa agttgaaatg gctgacttat cccttttccc ttatctatgc
    10981 gggactaact ggattttcag catcggttat tcgcagtctc ttgcaaaagc tactggctca
    11041 acatggggtt aagggcttgg ataattttgc cttgacggtg cttgtcctct ttattgtcat
    11101 gccaaacttt ttcttgacag caggaggagt cttgtcctgc gcttatgctt ttatcctgac
    11161 catgaccagc aaagaagggg aggggctcaa ggctgttact agtgaaagtc tagtcatctc
    11221 cttgggcata ttgcccattc tatccttcta ttttgcggaa tttcaacctt ggtctatcct
    11281 tttgaccttt gtcttttcct ttctttttga cttggtcttc ttaccgctct tgtctatctt
    11341 atttgtcctt tcctttctct atccagtcat tcagctgaac tttatctttg aatggttaga
    11401 gggcattatt cgcttggtct cgcaggtggc aaggagacca cttgtctttg gtcaacccaa
    11461 cgcatggctt ttaatcttat tgttaatttc cttggctttg gtctatgatt tgaggaaaaa
    11521 cattaaagga ttaacagtat tgagtttatt gattacaggt ctctttttcc ttaccaagta
    11581 tccactggaa aatgaaatca ccatgctgga tgtggggcaa ggagaaagta ttttcctacg
    11641 ggatgtaact gggaaaacca ttctcataga tgtaggtggt aaggcagaat cttataagaa
    11701 aatcaaaaaa tggcaagaaa agatgacgac cagcaatgcc cagcgaacct tgattcccta
    11761 tctcaaaagt cgaggagtag ctaagattga ccagctaatt ttgactaaca cggacaagga
    11821 gcatgttgga gatttgtcag agatgaccaa ggctttccat gtaggggaga ttctagtatc
    11881 aaaagacagt ctgaaacaga aggaatttgt ggcagaacta caggcgactc aaacaaaggt
    11941 gcgtagtatg atagtagggg agaacttgcc catttttgga agtcagttag aagttctatc
    12001 tccaaggaaa atgggagatg gaggacacga tgatacccta gttctgtatg ggaaattctt
    12061 ggataagcaa tttctcttca cgggaaattt ggaggagaaa ggagagaagg acttgctgaa
    12121 gcactatcca gacttgaaag taaatgtttt gaaagctagc caacatggca ataaaaaatc
    12181 atcaagtcca gcctttctag aaaaactcaa accagagctt actcttatct cagttggaaa
    12241 gagcaatcga atgaaactcc cccatcagga aacattgaca cgactggaag gtatcaatag
    12301 caaagtttat cgaactgacc agcaaggagc tatacgtttt aaggggttgg atagttggaa
    12361 aatcgaaagt gttcgatagg aaggataaat gttgtagatt agtgaaataa actaaaaatt
    12421 tgttgcataa taatgataaa aatggtataa tgaaaacgta ttcaatattg aggatataaa
    12481 atcattaaaa atcagcaaaa gttgttttat tagttagttt ataatctatt ggtcttcttc
    12541 agtccagtgt atctgctgtg acagtcacta aaagttacaa gtatgattgg aatacggttt
    12601 gggaatatag taccaactat cacgaccatc agtatgcttg gattccgtca tggtctcgtt
    12661 atgacagcta ttctgagtat aaagttggcg gaggctggaa ctacgctcgt tatgaggtca
    12721 taaactatta cagcggaggc tattaattct taaagagtga gaaaaaggag ggctagatat
    12781 gttgcagctt actcatgtga ccttaaaaac gcgacaagtc atcttgcaag atgtggattt
    12841 cacctttaaa aagggtaggg tttatggtct tcttgctatc aatggctctg gaaagacgac
    12901 cctgttccgt gccattagca atttaattcc cataagtagt ggaaatatcg cagcccctcc
    12961 ttctttattt tattatgaga gtattgaatg gctggatgga aacttaagtg ggatggacta
    13021 ccttcgtctt atcaaaaaca tctggaagtc aggtctgaac ttgagggatg aaatcgccta
    13081 ttgggaaatg tctgactata tcagtcttcc cattcgcaag tattccttag gcatgaagca
    13141 acgcttggtg attgccatgt atttcctcag tcaggccaaa tgctggctca tggatgagat
    13201 tacaaatggc ttagatgagt attatcgaca gaagtttttt gataggctag cacaaatcga
    13261 tagacaagaa cagctggttc ttttaagttc ccactataag gaagagttgg ttgatgtctg
    13321 cgatagagta gtaaccattc atcaggggca gatagaagag gtttagttta tgaaagatgt
    13381 tagtctattt ttattgaaaa aagttttcaa aagccgctta aactggattg tcttagcttt
    13441 atttgtatct gtactcggtg ttacctttta tttaaatagt cagactgcaa actcacacag
    13501 cttggagagc aggttggaaa gtcgcattgc agccaacgag agggctatca atgaaaatga
    13561 agagaaactc tcccaaatgt ctgataccag ctcggaggaa taccagtttg ctaaaaataa
    13621 tttagacgtg caaaaaaatc ttttgacgcg aaagacagaa attctgactt tattaaaaga
    13681 agggcgctgg aaagaagcct actatttgca gtggcaagat gaagagaaga attatgaatt
    13741 tgtatcaaat gacccgactg ctagccctgg cttaaaaatg ggggttgacc gcgaacggaa
    13801 gatttaccaa gccctgtatc ccttgaacat aaaagcacat actttggagt ttccgaccca
    13861 cgggattgat cagattgtct ggattttaga ggttatcatc ccaagtttgt ttgtggttgc
    13921 tattattttt atgctaacac aactatttgc agaaagatat caaaatcatc tggacacagc
    13981 tcacttatat cctgtttcaa aagtgacatt tgcaatatcc tctcttggag ttggagtggg
    14041 atatgtaact gtgctgttta tcggaatctg tggcttttct tttctagtgg gaagtctgat
    14101 aagtggtttt ggacagttag attatcccta cccaatttat agcttagtga atcaagaagt
    14161 aactattggg aaaatacaag atgtattatt tcctggcttg ctcttagctt tcttagcctt
    14221 tatcgtcatt gtggaagttg tgtacttgat tgcttacttt ttcaagcaaa aaatgcctgt
    14281 cctctttctt tcactcattg ggattgttgg cttattgttt ggtatccaaa ccattcagcc
    14341 tcttcaaagg attgcacatc tgattccctt tacttacttg cgttcagtgg agattttatc
    14401 tggaagatta cctaagcaga ttgataatgt cgatctaaat tggagcatgg gaatggtctt
    14461 acttccttgc ctgattatct ttttgctatt gggaattcta tttattgaaa gatggggaag
    14521 ttcacagaaa aaagaatttt ttaatagatt ctagctttcc tataggtagg gaaaataagt
    14581 aaaaactaac atagagaggg aatcaacttg attctctctt tttgattcga aaaccaaacc
    14641 aaaatacaaa cacaaacttt tcaaaaaata actttttatc ttgacaagag ctagaaaact
    14701 tggtatcata taaaagttga gaaaagcaga agtgagagct tctcgccttg tgacattaag
    14761 ttgcctggcc ctacggatga aaagtttcga agaaacgcta tcataacgtg cgggcttgta
    14821 tatttacaag tccgctattg tttttctcta ataaaacaaa agaggtgaaa accatagcaa
    14881 agcaagactt attcatcaat gatgagattc gtgtacgtga agttcgcttg attggtcttg
    14941 aaggagaaca gctaggtatc aagccactca gtgaagcgca agctttggct gataacgcta
    15001 atgttgacct agtattgatt caaccccaag ccaaaccgcc tgttgcaaaa attatggact
    15061 acggtaagtt caaatttgag taccagaaga agcaaaaaga acaacgtaaa aaacaaagcg
    15121 ttgttactgt gaaagaagtt cgtctaagtc caacaattga caagggtgac tttgatacaa
    15181 aacttcgtaa tgcacgtaag ttccttgaaa aaggaaataa agttaaggta tctattcgct
    15241 ttaagggtcg tatgattacc cataaagaga ttggtgcaaa agttttagcc gagtttgctg
    15301 aagcaactca agatattgcc atcatcgaac aacgtgctaa aatggatgga cgtcaaatgt
    15361 tcatgcaatt ggcgccagcg actgacaaaa aataattgtc agaaagttaa ataaaggaga
    15421 aaaaatcatg ccaaaacaaa aaacacaccg cgcatcagct aaacgtttca aacgtacagg
    15481 ttctggtgga cttaaacgtt tccgtgctta cacttctcac cgtttccacg gaaaaactaa
    15541 gaaacaacgt cgtcatcttc gtaaagcatc tatggtgcat tcaggagatt acaaacgtat
    15601 caaagcaatg cttactcgct tgaaataata gcttgactgt aagtaaacaa ttctaggaaa
    15661 tatttggagg aaataaaaca tggcacgtgt taaaggtggc gttgtatcac gcaaacgtcg
    15721 taaacgtatt cttaaattag caaaaggtta ctatggagct aaacacatct tgttccgtac
    15781 tgcaaaagaa caagtaatga actcttacta ctatgcatac cgtgaccgtc gtcagaaaaa
    15841 acgtgacttc cgcaaattgt ggattactcg tatcaatgcg gcagctcgta tgaacggact
    15901 ttcatactca caattgatgc atggtttgaa attggctgag atcgaagtta accgtaaaat
    15961 gcttgctgac ttggctgtta acgatgcagt agctttcaca gctcttgcag atgcagctaa
    16021 agcaaaactt ggtaaataac aactgatgag actggaacag gattgtccag tcttttttaa
    16081 aataaaggag aaagatatgg cttcaaaaat gctacacact tgcttacgag tagaaaatct
    16141 tgaaaaatca atcgcatttt atcaagatgc ttttggtttt aaagaattgc gtcgcagaga
    16201 ttttccagac catgccttca cgattgtcta tctaggtctt gagggcgatg actatgagtt
    16261 ggagttgact tataactacg atcacggtcc gtatgtggtt ggtgatgggt ttgcccatat
    16321 cgccctcagt acacctgatc ttgaggcact tcatcaagaa cacagtgcaa aaggctatga
    16381 agtgactgag ccaaatggtc taccaggaac tacaccaaac tattactttg tcaaagaccc
    16441 tgatggatat aaggtcgaag tcattcgtga aaaataattt cgtgcaagtt aagtagaata
    16501 ttcgttttct acttgacttt ttttagctga aagagtatac taataaaaat tcaaccttta
    16561 aggggagtcc agagagactc acaaggtgtc agataaaaga atggtgcaat tttctagagg
    16621 agactttttg agtgtgctct cttgtgttgt acgattttaa ctgaggcctt gcactagcaa
    16681 ggtcttttct ttatctggtc cccttaaaat ttaaggagga aaagttatga atcccacatg
    16741 taagaagcgt ttgggtgtca ttcggttgga aaccatgaag gtggttgcac aagaggaaat
    16801 cgcgccagca atctttgaat tagtcctaga aggagaaatg gttgaagcca tgcgagcagg
    16861 ccaatttctt catctgcgtg taccggacga tgcccatctc ttacgtcgtc ctatttcaat
    16921 ttcgtctatt gacaaggcaa acaagcagtg tcacctcatt tatcggattg acggagctgg
    16981 gactgcaatt ttttcaacct taagtcaggg agacactctt gatgtgatgg ggcctcaggg
    17041 aaatggtttt gacttgtctg accttgatga gcagaatcag gttctccttg ttggtggtgg
    17101 gattggtgtt ccacccttgc ttgaggtggc caaggaattg catgaacgtg gagtgaaagt
    17161 agtgacagtc ctcggttttg ctaataagga tgctgttatt ttgaaaacgg aattggctca
    17221 gtatggtcag gtctttgtaa cgacagatga tggttcttat ggcatcaagg gaaatgtttc
    17281 cgttgttatc aatgatttag acagtcagtt tgatgctgtt tactcgtgtg gggctccagg
    17341 aatgatgaag tatatcaatc aaacctttga tgatcaccca agagcctatt tatctctgga
    17401 atctcgtatg gcttgtggga tgggagcttg ctatgcctgt gttctaaaag taccagaaaa
    17461 cgagacggtc agccaacgcg tctgtgaaga tggtcctgtt ttccgcacag gaacagttgt
    17521 attataagga gaaaattatg actacaaatc gattacaagt ttctctacct ggtttggatt
    17581 tgaaaaatcc gattattcca gcatcaggct gttttggctt tggacaagag tatgccaagt
    17641 actatgattt agacctttta ggttctatta tgatcaaggc gacaaccctt gaaccacgtt
    17701 ttgggaatcc aactccaaga gtggcagaga cgcctgctgg tatgctcaat gcaattggct
    17761 tgcaaaatcc tggtttagag gttgttttgg ctgaaaagct accttggctg gaaagagaat
    17821 atccaaatct tcctattatt gccaatgtag ctggtttttc aaaacaagag tatgcagctg
    17881 tttctcatgg gatttccaag gcaactaatg taaaagctat cgagctcaat atttcttgtc
    17941 ccaatgttga ccactgtaat catggacttt tgattggtca agatccagat ttggcttatg
    18001 atgtggtgaa agcagctgtg gaagcctcag aagtgccagt ttatgtcaaa ttaaccccga
    18061 gtgtgaccga tatcgttact gtcgcaaaag ctgcagaaga tgcgggagca agtggcttga
    18121 ccatgatcaa tactctggtt ggaatgcgct ttgacctcaa aactagaaaa ccaatcttgg
    18181 ccaatggaac aggtggaatg tctggtccag cagtctttcc agtagccctc aaactcatcc
    18241 gccaagttgc ccaaacaaca gacctgccta tcattggaat gggaggagtg gattcggctg
    18301 aagctgccct agaaatgtat ctggctgggg catctgctat cggagttgga acagctaact
    18361 ttaccaatcc ttatgcctgc cctgacatca tcgaaaattt accaaaagtc atggataaat
    18421 acggtattag cagtctggaa gaactccgtc aggaagtaaa agagtctctg aggtaaactg
    18481 caatcaatct gttcttgatt ttttattagt ttgtaatatg aatttaggag aattttggta
    18541 caataaaata aataagaaca gaggaagaag gttaatgaag aaagtaagat ttattttttt
    18601 agctctgcta tttttcttag ctagtccaga gggtgcaatg gctagtgatg gtacttggca
    18661 aggaaaacag tatctgaaag aagatggcag tcaagcagca aatgagtggg tttttgatac
    18721 tcattatcaa tcttggttct atataaaagc agatgctaac tatgctgaaa atgaatggct
    18781 aaagcaaggt gacgactatt tttacctcaa atctggtggc tatatggcca aatcagaatg
    18841 ggtagaagac aagggagcct tttattatct tgaccaagat ggaaagatga aaagaaatgc
    18901 ttgggtagga acttcctatg ttggtgcaac aggtgccaaa gtaatagaag actgggtcta
    18961 tgattctcaa tacgatgctt ggttttatat caaagcagat ggacagcacg cagagaaaga
    19021 atggctccaa attaaaggga aggactatta tttcaaatcc ggtggttatc tactgacaag
    19081 tcagtggatt aatcaagctt atgtgaatgc tagtggtgcc aaagtacagc aaggttggct
    19141 ttttgacaaa caataccaat cttggtttta catcaaagaa aatggaaact atgctgataa
    19201 agaatggatt ttcgagaatg gtcactatta ttatctaaaa tccggtggct acatggcagc
    19261 caatgaatgg atttgggata aggaatcttg gttttatctc aaatttgatg ggaaaatggc
    19321 tgaaaaagaa tgggtctacg attctcatag tcaagcttgg tactacttca aatccggtgg
    19381 ttacatgaca gccaatgaat ggatttggga taaggaatct tggttttatc tcaaatctga
    19441 tgggaaaata gctgaaaaag aatgggtcta cgattctcat agtcaagctt ggtactactt
    19501 caaatccggt ggttacatga cagccaatga atggatttgg gataaggaat cttggtttta
    19561 cctcaaatct gatgggaaaa tagctgaaaa agaatgggtc tacgattctc atagtcaagc
    19621 ttggtactac ttcaaatctg gtggctacat ggcgaaaaat gagacagtag atggttatca
    19681 gcttggaagc gatggtaaat ggcttggagg aaaaactaca aatgaaaatg ctgcttacta
    19741 tcaagtagtg cctgttacag ccaatgttta tgattcagat ggtgaaaagc tttcctatat
    19801 atcgcaaggt agtgtcgtat ggctagataa ggatagaaaa agtgatgaca agcgcttggc
    19861 tattactatt tctggtttgt caggctatat gaaaacagaa gatttacaag cgctagatgc
    19921 tagtaaggac tttatccctt attatgagag tgatggccac cgtttttatc actatgtggc
    19981 tcagaatgct agtatcccag tagcttctca tctttctgat atggaagtag gcaagaaata
    20041 ttattcggca gatggcctgc attttgatgg ttttaagctt gagaatccct tccttttcaa
    20101 agatttaaca gaggctacaa actacagtgc tgaagaattg gataaggtat ttagtttgct
    20161 aaacattaac aatagccttt tggagaacaa gggcgctact tttaaggaag ccgaagaaca
    20221 ttaccatatc aatgctcttt atctccttgc ccatagtgcc ctagaaagta actggggaag
    20281 aagtaaaatt gccaaagata agaataattt ctttggcatt acagcctatg atacgacccc
    20341 ttacctttct gctaagacat ttgatgatgt ggataaggga attttaggtg caaccaagtg
    20401 gattaaggaa aattatatcg ataggggaag aactttcctt ggaaacaagg cttctggtat
    20461 gaatgtggaa tatgcttcag acccttattg gggcgaaaaa attgctagtg tgatgatgaa
    20521 aatcaatgag aagctaggtg gcaaagatta gtactataag tgaatatgat ttgagtgaat
    20581 agtaagttaa aaatcctgat ttcaagtaaa atcaggattt tttcatggat gcaatttttt
    20641 tggagtctgg tgtgacgcgg agggtctttt gtcctgtgta agtgacaaag ccgggttttc
    20701 caccagttgg tttattgagt tttttgactt caatcatatc tacctgcacc agattcgaca
    20761 ggcgcccttg agagaagtag gcagctaact ctgctgcgtc tgtcttgact gcatcagatg
    20821 ggtcaagatt tcctgagatg acaacatggc ttccaggaat gtccttagca tggaaccaaa
    20881 gttcctcctt gcgggccatt ttaaaggtca attcctcatt ttgaagattg tttcgtccga
    20941 catagatgat ggttttgcca tcgcttgcta gatattgttc tagttttttg cgtttctgga
    21001 ttttctcccg ttgtcttctg cggataaaac ctgtttgaat caattcttca cggatttcag
    21061 cgatttcttc cagtccagct tggttgagga cggtttctac actttccaga tagagaatag
    21121 tggctttggt ttcttcaatc aaatcagtca agtatttgac agcttctttg agtttctgat
    21181 accgtttaaa atagcgttgg gcattctggt tgggagtcag agccttatca agcgcaatca
    21241 tgataggttg gttggtatag tagttgtcta ggataacctg gtcttggtcg ttaggcactt
    21301 ggtggaggaa ggttgtcagc aattctcctt tttgacgaaa ttcttcagcg ttgtctgtcg
    21361 ccagtaactc tttttcctgt tttttgagtt tgtgtcggtt tttctgaagt tcattttcaa
    21421 cacgacgaat cagttcactg gcctgctgtt tgacgcggtc gcgctcagcc ttatccttat
    21481 agtaggtgtc caacaaatca gaaagatttg caaaaggctc tcccacctga tttgcaaaag
    21541 gaactggact gaaggaagtc tcagtcaagc atggcttggt ttcttgattg aaaaaatttc
    21601 ggaaagcgga aagtttttca ctaaccagta tcctttccaa ttcatttgcc gtatcgcgtc
    21661 ccagaccttg aaagaggctt tgaagatttt ttgctgttag ttcttgggtt tgcaggattt
    21721 caaagagctt ttcatccttg atagtaaaag gattgagaga ttttgtactt ggcggagcga
    21781 tataggtcga tcctggaagt aaggtgcggt agctattttg tgaaaagccg acgtgtttga
    21841 taacttcgag gattttatga ctgcttttat cgaccagtag aatattactg tgtttcccca
    21901 taatttcgat aatcaaggta gcctggatat ggtctccaat ctcgttttta ttggaaactg
    21961 taatttccac aatacggtca ttttccactt gctcaatcga ctcaatcagg gccccctgca
    22021 aatactttct caaaaccatg ataaaggtag aaggttgagc tggattttca aaagtcgttt
    22081 gggtcagctg aatgcgtcca aaaactggat gggcagaaag gagcaggcga tggctttggc
    22141 gattgctgcg gatttgcaag accaactctt gttcaaaagg ctgattgatt ttctggatgc
    22201 gaccattcac taattcgctt cgcaattcct caactatgtg gtgtaaaaaa aatccgtcaa
    22261 atgacatcgt tctctccttg tgattgtatt ccatagtatt atatcaaaaa ggtagaataa
    22321 aatcatggaa atgtggtata ataaagccaa gtaaagagaa acgagaagca catgtatatt
    22381 gaaatggtag atgaaactgg tcaagtttca aaagaaatgt tgcaacaaac ccaagaaatt
    22441 ttggaatttg cagcccaaaa attaggaaaa gaagacaagg agatggcagt cacttttgtg
    22501 accaatgagc gtagtcatga acttaatctg gagtaccgta acaccgaccg tccgacagat
    22561 gtcatcagcc ttgagtataa accagaattg gaaattgcct ttgacgaaga ggatttgctt
    22621 gaaaattcag aattggcaga gatgatgtct gagtttgatg cctatattgg ggaattgttc
    22681 atctctatcg ataaggctca tgagcaggcc gaagaatatg gtcacagctt tgagcgtgag
    22741 atgggcttct tggcagtaca cggcttttta catattaacg gctatgatca ctacactccg
    22801 gaagaagaag cggagatgtt cggtttacaa gaagaaattt tgacagccta tggactcaca
    22861 agacaataaa cgaaaatgga aaaatcgtga cttgatatcc agtttagaat ttgctttgac
    22921 aggtattttt actgctatca aggaagaacg caatatgcga aaacacgcag tgacggctct
    22981 agtggtcatc cttgcaggtt ttgtttttca ggtgtcacga atcgaatggc tctttctcct
    23041 attgagtatt ttcttggtag tagcctttga gattatcaac tctgctattg aaaatgtggt
    23101 ggatttggcc agtcactatc acttttccat gctggctaaa aatgccaagg atatggcggc
    23161 cggcgcggta ttagtggttt ctcttttcgc agccttaaca ggcgcattga tttttctccc
    23221 acgaatctgg gatttattat tttaaacagt aagaggaaat tatgactttt aaatcaggct
    23281 ttgtagccat tttaggacgt cccaatgttg ggaagtcaac ctttttaaat cacgttatgg
    23341 ggcaaaagat tgccatcatg agtgacaagg cgcagacaac gcgcaataaa atcatgggaa
    23401 tttacacgac tgataaggag caaattgtct ttatcgacac accagggatt cacaagccta
    23461 aaacagctct cggagatttc atggttgagt ctgcctacag tacccttcgc gaagtggaca
    23521 ctgttctttt catggtgcct gctgatgaag cgcgtggtaa gggggacgat atgattatcg
    23581 agcgtctcaa ggctgccaag gttcctgtga ttttggtggt gaataaaatc gataaggtcc
    23641 atccagacca gctcttgtct cagattgatg acttccgtaa tcaaatggac tttaaggaaa
    23701 ttgttccaat ctcagccctt cagggaaata acgtgtctcg tctagtggat attttgagtg
    23761 aaaatctgga tgaaggtttc caatatttcc cgtctgatca aatcacagac catccagaac
    23821 gtttcttggt ttcagaaatg gttcgcgaga aagtcttgca cctaactcgt gaagagattc
    23881 cgcattctgt agcagtagtt gttgactcta tgaaacgaga cgaagagaca gacaaggttc
    23941 acatccgtgc aaccatcatg gtcgagcgcg atagccaaaa agggattatc atcggtaaag
    24001 gtggcgctat gcttaagaaa atcggtagca tggcccgtcg tgatatcgaa ctcatgctag
    24061 gagacaaggt cttcctagaa acctgggtca aggtcaagaa aaactggcgc gataaaaagc
    24121 tagatttggc tgactttggc tataatgaaa gagaatacta agtagaggta ggctcatgcc
    24181 tgcttcttgt ttttacagaa ggaggactta tgcctgaatt acctgaggtt gaaaccgttt
    24241 gtcgtggctt agaaaaattg attataggaa agaagatttc gagtatagaa attcgctacc
    24301 ccaagatgat taagacggat ttggaagagt ttcaaaggga attgcctagt cagattatcg
    24361 agtcaatggg acgtcgtgga aaatatttgc ttttttatct gacagacaag gtcttgattt
    24421 cccatttgcg gatggagggc aagtattttt actatccaga ccaaggacct gaacgcaagc
    24481 atgcccatgt tttctttcat tttgaagatg gtggcacgct tgtttatgag gatgttcgca
    24541 agtttggaac catggaactc ttggtgcctg accttttaga cgtctacttt atttctaaaa
    24601 aattaggtcc tgaaccaagc gaacaagact ttgatttaca ggtctttcaa tctgcccttg
    24661 ccaagtccaa aaagcctatc aaatcccatc tcctagacca gaccttggta gctggacttg
    24721 gcaatatcta tgtggatgag gttctctggc gagctcaggt tcatccagct agaccttccc
    24781 agactttgac agcagaagaa gcgactgcca ttcatgacca gaccattgct gttttgggcc
    24841 aggctgttga aaaaggtggc tccaccattc ggacttatac caatgccttt ggggaagatg
    24901 gaagcatgca ggactttcat caggtctatg ataagactgg tcaagaatgt gtacgctgtg
    24961 gtaccatcat tgagaaaatt caactaggcg gacgtggaac ccacttttgt ccaaactgtc
    25021 aaaggaggga ctgatgggaa aaatcatcgg aatcactggg ggaattgcct ctggtaagtc
    25081 aactgtgaca aattttctaa gacagcaagg ctttcaagta gtggatgccg acgcagtcgt
    25141 ccaccaacta cagaaacctg gtggtcgtct gtttgaggct ctagtacagc actttgggca
    25201 agaaatcatt cttgaaaacg gagaactcaa tcgccctctc ctagctagtc tcatcttttc
    25261 aaatcctgat gaacgagaat ggtctaagca aattcaaggg gagattatcc gtgaggaact
    25321 ggctactttg agagaacagt tggctcagac agaagagatt ttcttcatgg atattcccct
    25381 actttttgag caggactaca gcgattggtt tgctgagact tggttggtct atgtggaccg
    25441 agatgcccaa gtggaacgct taatgaaaag ggaccagttg tccaaagatg aagctgagtc
    25501 tcgtctggca gcccagtggc ctttagaaaa aaagaaagat ttggccagcc aggttcttga
    25561 taataatggc aatcagaacc agcttcttaa tcaagtgcat atccttcttg agggaggtag
    25621 gcaagatgac agagattaac tggaaggata atctgcgcat tgcctggttt ggtaattttc
    25681 tgacaggagc cagtatttct ttggttgtac cttttatgcc catcttcgtg gaaaatctag
    25741 gtgtagggag tcagcaagtc gctttttatg caggcttagc aatttctgtc tctgctattt
    25801 ccgcggcgct cttttctcct atttggggta ttcttgctga caaatacggc cgaaaaccca
    25861 tgatgattcg ggcaggtctt gctatgacta tcactatggg aggcttggcc tttgtcccaa
    25921 atatctattg gttaatcttt cttcgtttac taaacggtgt atttgcaggt tttgttccta
    25981 atgcaacggc actgatagcc agtcaggttc caaaggagaa atcaggctct gccttaggta
    26041 ctttgtctac aggcgtagtt gcaggtactc taactggtcc ctttattggt ggctttatcg
    26101 cagaattatt tggcattcgt acagttttct tactggttgg tagttttcta tttttagctg
    26161 ctattttgac tatttgcttt atcaaggaag attttcaacc agtagccaag gaaaaggcta
    26221 ttccaacaaa ggaattattt acctcggtta aatatcccta tcttttgctc aatctctttt
    26281 taaccagttt tgtcatccaa ttttcagctc aatcgattgg ccctattttg gctctttatg
    26341 tacgcgactt agggcagaca gagaatcttc tttttgtctc tggtttgatt gtgtccagta
    26401 tgggcttttc cagcatgatg agtgcaggag tcatgggcaa gctaggtgac aaggtgggca
    26461 atcatcgtct cttggttgtc gcccagtttt attcagtcat catctatctc ctctgtgcca
    26521 atgcctctag cccccttcaa ctaggactct atcgtttcct ctttggattg ggaaccggtg
    26581 ccttgattcc cggggttaat gccctactca gcaaaatgac tcccaaagcc ggcatttcga
    26641 gggtctttgc cttcaatcag gtattctttt atctgggagg tgttgttggt cccatggcag
    26701 gttctgcagt agcaggtcaa tttggctacc atgctgtctt ttatgcgaca agcctttgtg
    26761 ttgcctttag ttgtctcttt aacctgattc aatttcgaac attattaaaa gtaaaggaaa
    26821 tctagtgcga gtaaaaatca atctcaaatg ctcctcttgt ggcagtatca attacctaac
    26881 cagtaaaaat tcaaaaaccc atccagacaa gattgaggtt ttaaagtatt gtcccaagga
    26941 aagaaaagta accctacatc ttgaatctaa gtagatttta tggtaaaata atagggattt
    27001 taaggagttt gatatgtata acctattatt aaccatttta ttagtattat ctgttgtgat
    27061 tgtgattgca attttcatgc aaccaaccaa aaaccaatcc agcaatgtat ttgatgccag
    27121 ttcaggtgat ttgtttgaac gcagtaaagc tcgcggtttt gaagctgtaa tgcagcgttt
    27181 gacagggatt ttagtctttt tctggctagc cattgcctta gcattgacgg tattatcaag
    27241 tagataagaa aataatgggc aggactaggt ctttgcctct ttttattttt aaaggatgtt
    27301 tgagaaggtt ttacagtaaa agaaaattaa aaaatctaga aagaaaatat gaaagataga
    27361 ataaaagaat atttacaaga caagggaaag gtgactgtta atgatttggc tcaggctttg
    27421 ggaaaagaca gttccaagga ttttcgtgag ttgattaaaa ccttgtcctt aatggaaaga
    27481 aagcaccaaa ttcgttttga agaagatggt agtctgacat tagaaattaa gaaaaaacat
    27541 gagattaccc tcaaggggat ttttcatgcc cataaaaatg gctttggctt tgttagtctg
    27601 gaaggcgagg aggacgacct ttttgtaggg aaaaatgatg tcaactatgc tattgatggt
    27661 gataccgtcg aggtagtgat taagaaagtc gctgaccgca ataagggaac agcagcagaa
    27721 gccaaaatta ttgatatcct agaacacagt ttgacaacag ttgtcgggca aatcgttctg
    27781 gatcaggaaa aacctaagta tgctggctat attcgttcaa aaaatcagaa aatcagtcaa
    27841 ccgatttatg ttaagaaacc agccctaaaa ttagaaggaa cagaagttct caaagtcttt
    27901 atcgataaat acccaagcaa gaaacatgat ttctttgtcg cgagtgttct cgatgtagtg
    27961 ggacactcaa cggatgtcgg aattgatgtt cttgaggtct tggaatcaat ggacattgta
    28021 tccgagtttc cagaagctgt tgttaaggaa gcagaaagtg tgcctgatgc tccgtctcaa
    28081 aaggatatgg aaggtcgtct ggatctaaga gatgaaatta cctttaccat tgacggtgcg
    28141 gatgccaagg acttggacga tgcagtgcat atcaaggctc tgaaaaatgg caatctggag
    28201 tttggggttc acatcgcaga tgtttcttat tatgtgaccg aggggtctgc ccttgacaag
    28261 gaagccctta accgtgcgac ttctgtttac gtgacagacc gagtggtgcc aatgcttcca
    28321 gaacgactat caaatggcat ctgctctctc aatccccaag ttgaccgcct gacccagtct
    28381 gctattatgg agattgataa acatggtcgt gtggtcaact ataccattac acaaacagtt
    28441 atcaagacca gttttcgtat gacctatagc gatgtcaatg atatcctagc tggcgatgaa
    28501 gaaaagagaa aagaatatca taaaattgta tcaagtatcg aactcatggc caagcttcat
    28561 gaaactttag aaaacatgcg tgtgaaacgt ggagctctca attttgatac caatgaagcg
    28621 aagattttag tggataaaca aggtaagcct gttgatatcg ttcttcggca gcgtggtatt
    28681 gccgagcgga tgattgagtc ttttatgttg atggctaatg aaacagttgc cgaacatttc
    28741 agcaagttgg atttgccttt tatctatcga attcacgagg agcctaaggc tgaaaaggtt
    28801 cagaagttta ttgattatgc ttcgagtttt ggcttgcgca tttatggaac tgccagtgag
    28861 attagtcagg aggcacttca agacatcatg cgtgctgttg agggagaacc ttatgcagat
    28921 gtattgtcca tgatgcttct tcgttctatg cagcaggctc gttattcgga gcacaatcac
    28981 ggccactatg gactagctgc tgactattat actcacttta ccagtccaat tcgtcgttat
    29041 ccagaccttc ttgttcaccg tatgattcgg gattacggcc gttctaagga aatagcagag
    29101 cattttgaac aagtgattcc agagattgcg acccagtctt ccaaccgtga acgtcgtgcc
    29161 atagaagctg agcgtgaagt cgaagccatg aaaaaggctg agtatatgga agaatacgtg
    29221 ggtgaagagt atgatgcagt tgtatcaagt attgtcaaat tcggtctctt tgtcgaattg
    29281 ccaaacacag ttgaaggctt gattcacatc actaatctgc ctgaatttta tcatttcaat
    29341 gagcgtgatt tgactcttcg tggagaaaaa tcaggtatca ctttccgagt gggtcagcag
    29401 atccgtatcc gtgttgaaag agcggataaa atgactggag agattgattt ttcattcgta
    29461 cctagtgagt ttgatgtgat tgaaaaaggc ttgaaacagt ctagtcgtag tggcagaggg
    29521 cgtgattcaa atcgtcgttc ggataagaag gaagacaaga gaaaatcagg acgctcaaat
    29581 gataagcgta agcattcaca aaaagacaag aagaaaaaag gaaagaaacc tttttacaag
    29641 gaagtagcta agaaaggagc caagcatggc aaagggcgag ggaaaggtcg tcgcacaaaa
    29701 taaaaaggca cgccacgact atacaatcgt agatacgcta gaggcaggga tggtcctgac
    29761 tggaactgaa atcaagagtg tacgagctgc tcgaattaat ctcaaggatg gctttgctca
    29821 agtgaaaaat ggagaagttt ggctgagcaa tgttcatatc gcgccttacg aagagggcaa
    29881 tatctggaac caggaaccag aacgtcgtcg taaactcctg ctccataaaa agcaaattca
    29941 aaaattggaa caagagatca aagggacagg aatgacctta gttcccctta aggtctatat
    30001 aaaagatggc tacgctaagc ttcttttagg acttgccaaa gggaagcatg actatgacaa
    30061 acgggagtct atcaaacgtc gtgagcaaaa tcgagatatc gcgcgtgtga tgaaagctgt
    30121 taatcagcga taaaaagagg aattgaaaat ggaaaaatta gttgcctata aacgcatgcc
    30181 tttgtggaat aaacaaacaa tgcctgaagc tgttcagcaa aagcacaata caaaagttgg
    30241 gacttggggg aaaattactg tcttgaaggg agctctcaag tttattgaat tgacagaaga
    30301 aggggaagtt ctagctgaac acctctttga agcaggggca gacaatccaa tggcccaacc
    30361 tcaagcctgg caccgagtgg aagctgccac agatgatgtg gaatggtact tggaatttta
    30421 ttgtaaacct gaggattatt ttgctaaaaa atacaatacc aatcctgttc attcagaggt
    30481 cctagaggcc atgcagacag tgaaacaagg gaaagctttg gatttgggtt gtggtcaggg
    30541 gcgtaattct ctttttctag cccagcaaga ttttgatgtg acggctgtag atcaaaatgg
    30601 actagctctt gaaatcttgc aaagcattgt ggagcaggaa gatttggaca tgcctgttgg
    30661 cctttacgat atcaattcag ctagcattga acaagaatat gattttatcg tttcaacagt
    30721 tgttctcatg tttctacaag cggaccgcat tccagctatt attcaaaata tgcaggagaa
    30781 aaccagtgtt ggtggttaca accttatcgt ttgtgccatg gacacggagg attatccttg
    30841 ctcggttaac ttcccattca cctttaaaga aggagaactg gcagactatt acaaggattg
    30901 ggaattggtt aagtacaatg aaaatccagg ccatttgcac cgtcgcgatg agaatggcaa
    30961 tcgtattcaa ctacgctttg cgaccttact agctaagaaa atcaagtaaa cacacatgaa
    31021 gattaggaat tttcctgatc ttt
//
LOCUS       DBDESG010000023        30556 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.023, whole genome shotgun sequence.
ACCESSION   DBDESG010000023 DBDESG010000000
VERSION     DBDESG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.023"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..599)
                     /locus_tag="ACKB38_000996"
     CDS             complement(<1..599)
                     /locus_tag="ACKB38_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGL7979778.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAE"
     gene            complement(618..1721)
                     /gene="alr"
                     /locus_tag="ACKB38_000997"
     CDS             complement(618..1721)
                     /gene="alr"
                     /locus_tag="ACKB38_000997"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGL7979779.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(1711..2073)
                     /gene="acpS"
                     /locus_tag="ACKB38_000998"
     CDS             complement(1711..2073)
                     /gene="acpS"
                     /locus_tag="ACKB38_000998"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGL7979780.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            complement(2118..3149)
                     /locus_tag="ACKB38_000999"
     CDS             complement(2118..3149)
                     /locus_tag="ACKB38_000999"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGL7979781.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(3151..4182)
                     /locus_tag="ACKB38_001000"
     CDS             complement(3151..4182)
                     /locus_tag="ACKB38_001000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGL7979782.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(4263..6776)
                     /gene="secA"
                     /locus_tag="ACKB38_001001"
     CDS             complement(4263..6776)
                     /gene="secA"
                     /locus_tag="ACKB38_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGL7979783.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(6922..7209)
                     /locus_tag="ACKB38_001002"
     CDS             complement(6922..7209)
                     /locus_tag="ACKB38_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979784.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(7237..7869)
                     /locus_tag="ACKB38_001003"
     CDS             complement(7237..7869)
                     /locus_tag="ACKB38_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7979785.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(7873..8514)
                     /locus_tag="ACKB38_001004"
     CDS             complement(7873..8514)
                     /locus_tag="ACKB38_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979786.1"
                     /translation="MFKNFNNILLNRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(8507..9298)
                     /locus_tag="ACKB38_001005"
     CDS             complement(8507..9298)
                     /locus_tag="ACKB38_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979787.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(9483..9818)
                     /locus_tag="ACKB38_001006"
     CDS             complement(9483..9818)
                     /locus_tag="ACKB38_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979788.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(9958..10293)
                     /locus_tag="ACKB38_001007"
     CDS             complement(9958..10293)
                     /locus_tag="ACKB38_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979789.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(10382..11692)
                     /gene="der"
                     /locus_tag="ACKB38_001008"
     CDS             complement(10382..11692)
                     /gene="der"
                     /locus_tag="ACKB38_001008"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGL7979790.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(11706..12419)
                     /locus_tag="ACKB38_001009"
     CDS             complement(11706..12419)
                     /locus_tag="ACKB38_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGL7979791.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(12416..13312)
                     /gene="dnaI"
                     /locus_tag="ACKB38_001010"
     CDS             complement(12416..13312)
                     /gene="dnaI"
                     /locus_tag="ACKB38_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGL7979792.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(13313..14482)
                     /locus_tag="ACKB38_001011"
     CDS             complement(13313..14482)
                     /locus_tag="ACKB38_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGL7979793.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(14483..14956)
                     /gene="nrdR"
                     /locus_tag="ACKB38_001012"
     CDS             complement(14483..14956)
                     /gene="nrdR"
                     /locus_tag="ACKB38_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGL7979794.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15096..15461
                     /locus_tag="ACKB38_001013"
     CDS             15096..15461
                     /locus_tag="ACKB38_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGL7979795.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            15466..16161
                     /locus_tag="ACKB38_001014"
     CDS             15466..16161
                     /locus_tag="ACKB38_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979796.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            16178..16945
                     /locus_tag="ACKB38_001015"
     CDS             16178..16945
                     /locus_tag="ACKB38_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979797.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKSSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(17357..18556)
                     /locus_tag="ACKB38_001016"
     CDS             complement(17357..18556)
                     /locus_tag="ACKB38_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979798.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(18553..19446)
                     /locus_tag="ACKB38_001017"
     CDS             complement(18553..19446)
                     /locus_tag="ACKB38_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979799.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFSILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            19569..19760
                     /locus_tag="ACKB38_001018"
     CDS             19569..19760
                     /locus_tag="ACKB38_001018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979800.1"
                     /translation="MKIKEQTRKLAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            20153..20347
                     /locus_tag="ACKB38_001019"
                     /pseudo
     CDS             20153..20347
                     /locus_tag="ACKB38_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558115.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            20762..21379
                     /locus_tag="ACKB38_001020"
     CDS             20762..21379
                     /locus_tag="ACKB38_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGL7979801.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(21699..22586)
                     /gene="scrK"
                     /locus_tag="ACKB38_001021"
     CDS             complement(21699..22586)
                     /gene="scrK"
                     /locus_tag="ACKB38_001021"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGL7979802.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(22735..24618)
                     /locus_tag="ACKB38_001022"
     CDS             complement(22735..24618)
                     /locus_tag="ACKB38_001022"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGL7979803.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKRAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IVVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            24994..26448
                     /locus_tag="ACKB38_001023"
     CDS             24994..26448
                     /locus_tag="ACKB38_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGL7979804.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            26429..27394
                     /locus_tag="ACKB38_001024"
     CDS             26429..27394
                     /locus_tag="ACKB38_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7979805.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(27429..28703)
                     /locus_tag="ACKB38_001025"
     CDS             complement(27429..28703)
                     /locus_tag="ACKB38_001025"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGL7979806.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(28703..29875)
                     /locus_tag="ACKB38_001026"
     CDS             complement(28703..29875)
                     /locus_tag="ACKB38_001026"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGL7979807.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(30006..30254)
                     /locus_tag="ACKB38_001027"
     CDS             complement(30006..30254)
                     /locus_tag="ACKB38_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979808.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(30322..>30556)
                     /locus_tag="ACKB38_001028"
     CDS             complement(30322..>30556)
                     /locus_tag="ACKB38_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGL7979809.1"
                     /translation="KIYPQAQTPRKSSKLKPLTAEDKACNHALSKERSKVENIFAKVK
                     TFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT         8994 a         6780 c         5767 g         9015 t
ORIGIN      
        1 tattctgcca aatactttgg aaaatgcata gcacggactg cctgacaacg ggacatgagt
       61 ttgtatttgt ctagtaaaga ctggggcaga ttttcttcta tcaagaggtc cagtccctga
      121 tcaaaagccg tcttgatgac cttgaccaga ctggcctgac tgattccctg agccagacga
      181 tagacaggct ggaggtcatc ttctacctga gccagaacct tcatcccagt cagactagcc
      241 ttagcgcggt cccattttcc aaagacagca agggttgctc ccaactctat tttatcagcc
      301 agatagggct ggttaaagaa attcaccgca aaaacgacct ctccctgctt gagactaaaa
      361 cgcaggcgat tgcgcttgaa accataatac tggacactag caggagtcac tacctgacca
      421 gaaagaactg ccttctcacc gtcttccagc tccagcacct gcttggtttt gaagtcttca
      481 taacggaaag gaaagtagag caagagatct tgcaagtttt caattcctag tttggcgtat
      541 ttttctgctg actttggtcc cacaccaggc aagacatgca agggttgatg tagattcatg
      601 ctccactcct ttcttttcta ataatattct ctcggaatac ggtcgctgag gaggcaaacc
      661 acctcatagt taatggttac gcggtaggtc gctacctgag ttgcagtgat ttccttatcc
      721 ccattggagc caatcaaggt taccttggtt cctagcggat aaagcttagg caatcgaata
      781 gtgatttggt ccatcgaaac cctgccgaca attgggcaag cttggccatc taccaagaca
      841 gagaaatttt gcatgtctct tgtccatcca tctgcatacc cgattggcac ggtcgcgatg
      901 acttgctcgc tatccgcttg ataagttgct ccatagccca tgcaagctcc agctggaact
      961 gtcttgacat gaaccagagc agactccaag gtcaaggccg gtatcaaatc ataaggcaaa
     1021 tccaagaccg ctccacttgg attgaggcca tacatggcat ctcccatacg aaccgcattg
     1081 aaaatagtct ctacatgcca aagagtcgtt gcagaattgc tagcatgaac cagctctgga
     1141 acttccttca tactagctaa aatagtttta aaccgttcta actgggcatt aaaatagtca
     1201 tctgattcct catcagcagt agcaaagtgg gtaaagattc cttcaacaca aacaccgtgt
     1261 tgttggagca aatcttgagc ctgctcaacc tcacttgcct ctctaaaacc aatccgtccc
     1321 atccctgaat caatcttgag gtggactgtc aatccagtta ggtccacttc cttatctaag
     1381 agtgcttgaa tccactccag tccagccact gtcaaggtga agtcatattc tttagctaga
     1441 gcaacagctt cgatttcaga aactcctaaa atgaggattg gcttgctgag tccagcttgt
     1501 ctgagttcaa tggcttcatc gatattggaa acgcaaaagc catcaacatc atcttgaatt
     1561 gccttggcaa cggcaacagc tccatgacca taagcattgg ccttaaccac agccaacttg
     1621 agcgttcctt gagggatatg agcccccatt tgctgaatat tttgtcgaat agctcccaga
     1681 tgaatcagag ccttggttgg tctatgtgga ctagctttca tgattttcct ccaaaatgac
     1741 actggctgtc acaaactgat cggtgtggct gatagacagc caaatctttc ctgaaaatgg
     1801 tgcctgacta aaataaggcg ccccacgttc attgttcaag acttccaaat cctgaaaacc
     1861 gagcttgcta atgcccgttc ccatagcctt ggaaaaggcc tccttagccg accagcgacc
     1921 agctaaatat tctatttgcc tgcgtccttt gagactggtg aagcgctcca tttcctgagc
     1981 ggtcagtaca cgcttagcaa atccttcatg tcgtgtaact gcgctttcta tcgaagccaa
     2041 ttcttcgatg tcaattccgt gtccaactat cattctcatc aaaaggagtt gagattcccc
     2101 aactccatcc ttttcactta ttttgtcaag gtaacataaa tctcttctac caaggcctct
     2161 gtattttccc aacctaggca agggtcagta atagagcaac caaagatctc tggttggttt
     2221 tgacgaccat ctgctaggta agattcaatc ataaatcctc gaaccgtctt tttaattttc
     2281 tcattccaat cacgattctg caaggtctgg cgaacaattc gaatctgctc catatattgc
     2341 ttgcctgagt tatcatggtt ggtgtcaatg aggataaaag gattttcaag tcccatggtt
     2401 tcatagcgtt caatggcttg gagtagattt tcatagtagt aattcggcat ataattgcca
     2461 tactcgttga ctgctccacg gaggataaca tgggccaaag gattacctga tgtctcaact
     2521 tcctgcccat gataaaggaa ggtttgcttg ttttgagcag catagatggc gttaaacata
     2581 acacccaaat ttcctgaggt tggatttttc atccctactg gtgcatcaat cccagaagcc
     2641 acaaagcggt gctcttggtc ttccacagaa cgagctccaa cggcatggta gctgaccaag
     2701 tcatccacca agatcagatt tgacggataa agcatctcat ctgccgttgt caaaccagtc
     2761 tctgtaatca cgcggtagtg caactggcgc acagcctgca agccattaat caggcttgga
     2821 gccttagaag tatctggctg gtgaactaat cctttatagc cgtctccatt ggtacgaggc
     2881 ttagcagtat acacgcgcat gaccatgaaa atcttatccg ctaccttctt ttgcaaggca
     2941 gataaacggc gagcatattc caagaccgcc tcttcattat cagaagagca aggaccaatc
     3001 accaagagaa tacggtcatc ttcccctgaa ataatatctg ccaattccct gtcacggctt
     3061 tccttgagtc tcaaggcttc cgcagacaat tgggtttcag ccttgatgac ttccatatcg
     3121 atttcttgac ctttttcaat aaatgccatc ttattctcct aacgttttgt aaatttctct
     3181 gacaagagct tctgtgttat cccaacccag gcaagggtct gtgatagact taccaaatac
     3241 ttctggctca ttttgtcgac catcttccag ataagactca atcataaaac cacgaacgaa
     3301 ctgcttaatt ttttcattcc aagcacggtt aatcaaggtc tggcggacaa ttcggatctg
     3361 ttcaatatac tgcttaccag aattgtcatg attggtatca atgatgataa aaggattttc
     3421 caagcccatt ttctcatact gggcaatggt atcaattaaa ttgtcataat agtagttggg
     3481 aatatttttt ccatactcat taagagcacc acgaagaata gcgtgtgaaa gcgggttccc
     3541 agttgtttct acttcttttc ctaagaaaag gaaactttgt ttgttttgag cagcataaat
     3601 cccattaaac atgacattga gatttccaga ggttggattt ttaaaaccag tagaaaatcc
     3661 tgccccactt gccacaaagc ggtgttgctg gtcttcaact gaacgggcac caactgccat
     3721 gtaagaaatc aaatcatcta caagcggaag gttttcagga taaagcattt catcagcagt
     3781 tgtcatccct gtttctgtga tgacacgata gtgaagatgg cgaacggctt tgattccatt
     3841 gataagacta ggcgcttctg tcgcgttagg ctggtgaatc aagcccttat agccatctcc
     3901 gttggtacgg ggtttggcag tataaacacg cataaccata aagatacgat ctgccacttc
     3961 ttcttgtagg actgccaaac gcttagcgta ttcaaggaca gcttcttcgt tgtcagatga
     4021 gcatggcccg attaccaaga gaattcgctg gtcttctcca cgtataatgg cttctagctc
     4081 ttgatctcgc tgtgatttcc tctccaaagc ctgaccttct aattttgaca aggcacgaac
     4141 ttcttctata tttattttag agctttttgc tgtaaatacc ataactcatc tccttataaa
     4201 gcaaacggat acccagtaaa aatttacttt tcacaagata tccgcctcta aactatctca
     4261 ttttattgtc ttttaccgtg acagttttta aatttcttac cagaaccaca tgggcaaagt
     4321 tcattgcgtc caatctggct caaatccaaa tcttctggca tacttgcttg gtgagcagcg
     4381 atattgcgag tcgctgttgt actgatatga cgttctgcct gtggtctttc ttgttcatga
     4441 atttgtgctt tcatcatcaa gcgtgtcaca tcaaactcaa tcgaaccaat catatcatta
     4501 aacatacgga aaccttctgc ctgatactca acaacagggt tgttctgagc atagccacga
     4561 agtccaaccg cgttacgcaa ttgatcaagg gcatcgatat gatctgtcca cttgttatcc
     4621 accactcgta gaatcaaaac tttttggaat tctttaactg cttcttcatc gcgtagtttt
     4681 gaaacctgac tatcgtaaac cttcaaggaa cgttggaaaa gctcttcctt gatggcctta
     4741 tcagacaagc ctgacaagtc ttccatcgta atagaatctt caggaagcaa gttgtactta
     4801 gcaaagttca aaattgcctc tagtttttca tcttgtttgg cacgcgcatg accatcaacg
     4861 acacgttcaa tcgtgcgttt gatcatagac tgaatttcag gtgccaagtc acgatctgca
     4921 gtgatgacat cgtaacgttg agcatagata atctcacgtt gttcacgcat gacatcatcg
     4981 tattgaagga cttgtttacg ggtatcgtag ttatttcctt cgacacgttt ctgagctgct
     5041 tcaacctgac gcgtcaacat gcgagactca atggcctctt cagacatgtt caagcgttca
     5101 aagattccct tcaagcgttc agaaccaaaa cgtttcatca aatcatcttc aagagatagg
     5161 tagaattgtg actcacctgg atctccttga cgacctgaac gtccacgaag ctggttatcg
     5221 atacgacgac tttcatgacg ttctgtacca ataacacaaa gtcctccaag ttcacgaaca
     5281 ccttcaccaa gcttgatgtc ggtaccacga cccgccatgt tggttgcgat ggtaacggca
     5341 ccacgttgac cagcattcat gatgatttgg gcttctctat agtggttttt ggcattcaag
     5401 acttcgtgag gaacaccagc tgcaaccaat ttcttagaaa tgtagtcact agtttcaacc
     5461 gctactgtac caaccaagac aggttgaccc ttttggtaac gagccttaac gtcttcgaca
     5521 accgctttaa acttagattc gatacttgca taaagaaggt ctgagtggtc aatacgttga
     5581 acaggacggt ttgttgggat tggaataaca cgaatgttgt agatttcacg gaattcttct
     5641 tcctcagtct tacctgtacc cgtcatacca gacaatttct tgtacatacg gaagaggttt
     5701 tggtacgtga ttgaggcaga tgtcttggtt tcatcctgga ttggcacacc ttctttggct
     5761 tcaatagctt ggtgcaatcc atcagaataa cgacgacctt ccatggtacg acctgtaaat
     5821 tggtcgacaa tcaagatttc ttgctcttcg ctcaccacat agtcaatatc gagaagcatg
     5881 atgtagttgg cacgaagggc gttatcgata aagtgagtca aagccacgtt ttcgatgtca
     5941 tagaggtttt caagtttgaa gtagctttca gccctgtcaa tccctgaatc agacaaacca
     6001 atagtcttag actgcacatc gatgatgtag tcatctttgt tcaaagattt tacatagtgg
     6061 tctgccatgt gatacaactg actggtttca accgcattgg cacctgatac aatcaaaggt
     6121 gtacgagcct cgtcaatcaa gatagagtca acctcatcga ccaaggcata gttaagcgga
     6181 cgttgtacca tgttttcggc gcgaacgacc atgttgtcac gaaggtagtc aaatccgatt
     6241 tctgagttag ttgagtaagt aatatcacac tcataggctt cttttttctc cattggagat
     6301 ttggtagcca agttaatccc tactgacaaa ccaagccaag agtacaattc acccatctca
     6361 gtcgcgtcac gttctgacag gtattcatta accgtaacta cgtgaacccc tttacctgaa
     6421 agggcattga ggtataccgg catggtcgca gtcaaggttt tcccttcccc tgtacgcatc
     6481 tctggcacgt caccatggtg aagaacaatc ccccccatga cctgaacctt ataagggaag
     6541 agacctagga cacgtttggc accttcacgg acaaccgcaa atgcttcgta aagcaatgaa
     6601 tccagtgatt ctccattttg ataacgttcc ttaaattcaa ctgtttttgc ttttagttgg
     6661 tcgtcagtca aagcagccat ttggtcttcg tatttgaaaa ccttgtcagc catcttttcc
     6721 agacgacgga tttctccttt atcattttcg ataattgttt ttaaaatatt agccatgttt
     6781 ttccttactt taaattccga atattttaga atgttccttt aattttagca caattactga
     6841 ttattttcaa ggaagaatcc ccattttgaa aaggaaaaag aggttagttt tcaagctaac
     6901 ctctccgact tttatgactg tttaattgcc aaaaatcggc aaaatggcaa atgtgataaa
     6961 taagttaatc caaagagaaa gacagcaaat cagtccaaaa acaaagagac tgactttctt
     7021 ggacagcaag gccatgagaa tggacagaat tccaaaagct agcaagattt tctgtatgat
     7081 gaagatgtca atccttatcc ccagaagtgg tttatcccaa gggataaaga agaaagacag
     7141 ccagatcagc agaactaaac caatataaac cttggaatat cgtgtaataa atgattttag
     7201 atgtgccata agccatctcc taggattgta tatcctttac atttgtatat gtccattttc
     7261 gacaactaat acatctgtac aaaggtctga aatatcttcc gatatgtgcg acgtcaggat
     7321 aaccagacca ttttcttttt tcaggtaaga taaaatgacc tgtttggtta acctcacact
     7381 cttctcatcc aaagcattca taggttcatc gagaaagagt atagaaggag aggaaataaa
     7441 ggcttgaatc aaggccattt tttgctttgt tcctaaggat aaatgacggt attcaatgtc
     7501 ttcaaattcc tgtaaatcat agtattgaat ccaataggca attctttttt ccgtaacttt
     7561 agatgaaaaa taccttaaca gttccaaatt ttctctcagg gataaatgag ataaaaactc
     7621 gactttttca attaaaattc ctgcatcctg aatataatga ttttttaccc cgtaaacttt
     7681 accatcttga agaacttttc ctttgtcaag cttaatataa ccagcaagta tctttaaaag
     7741 aaccgtcttg ccagatccat tatcaccttt aagtccataa atttttcctg attgaaacac
     7801 caaattcaac tgatttaaaa taggtctttt cccataaatt ttctgtccat ttataatttc
     7861 aagtctcatc gtttatcctt tcttaaattt ctcttccaag cgactaacaa tcaacataac
     7921 taataaactt aagagtacta aatacactgt tacaatatga tccatcatcc attcttgtcc
     7981 cacaaatgct aaaaaataga gaaaaaagga aaaaccagcg cccgcactaa tcagtgctat
     8041 cagataagac agtagtaagt acaacatcat taactgagca agtaaaaaga aaagtgcgac
     8101 tgttttgaaa tgaattccta gaggaagtaa accaagaaac cagacaaaca aatcttgagt
     8161 aaaaaattta gaaatgacaa ccaatcgttt ccttgtataa tcgaaaaagg agcttgattg
     8221 atagcgaatc aagtgatggt aatggtctgc caaatcactt tcaagtaaaa tgacagagag
     8281 ccatcctacc atgaaaagcg aaaggtttaa caatagtttg gggatttcta aattcgcttc
     8341 agaatagtca ttataggaaa aaattgaaat caattctctc ttgaaaaaga taacagcatc
     8401 ctgcttttga accgctgttg acaatagatg gtttatcaaa atcatcatca gaactatacg
     8461 aagtagtaaa acaatctttc tatttagcaa aatattgtta aaattcttaa acattatctt
     8521 catatttttc aagcaccatg taagggacaa tataaaggat ataaggagta aagagtttca
     8581 tcaaacttac atccccatag ctagcttgca tcaaactcgt cataggaacc atatagtaag
     8641 gcaaggcact ataaagcagc atagaaccaa gccaaagaaa catcaaatag gtgattgccg
     8701 aacgagtcac attccccaca taatcaacta tttgtaaaaa atagattgca ttgatgaaaa
     8761 taccgattag taggacacaa gtaaagaaca aatagctttt tatctctcca tctaggagtc
     8821 tttgtaaacc acttccatca gaaaatagag aattccatcc aagaggagaa aaagtcccaa
     8881 agaaataggt tataattgca attatcagca cagtcactaa atatatcaaa caagggatac
     8941 ttgcaacttg caaagtcaac tttcttttta acccttgata actcacttct cttccaatac
     9001 tcaatcgtaa aactttattt ttcagctcaa tatattgaaa acctagcaaa acaatgatga
     9061 ttggaataat aaatgattga taaaaaccta gattgaactg gagaatacga aaatctaatg
     9121 ctgattttaa taagtcattt ttaattgctt gtccatcgaa tttagctaga tcgggcgcat
     9181 gaatatcaaa aaagaaataa gtcatcaaaa catcacgata aaccaaagca agaaaaagca
     9241 aagtaagcaa gacataaaca aatttatgcc ttgcaaaagt taagagagga atcttcataa
     9301 gccacctcct acaaatagaa agtaatataa tactcaatga aaatcaaaga gcaaactagg
     9361 aaactagccg caagttgctc aaagcactgc tttgaggttg tagataagac tgacgaagtc
     9421 agttacatat ataatccaag gcgacgttga cgtggtttga agagattttc gaagagtata
     9481 aatcaatgcc ttccgccttt agacttccct agttcccgtc tcaagtcaag catttttttg
     9541 aaaaagacat aggctaacca gtcactagca aaagcgataa gtagtaataa tagccaaata
     9601 ccccataact tgatatctgt caaatttctc aagacgatat tgaaaaacag aactgaaaaa
     9661 actgtccaag caaggctaaa aagagcatag agggtgatgt aaatccagta aaaatagtaa
     9721 aaaattggga aaaatttact ttctctatta tccttgtcaa tccagctgaa aaagtaaaaa
     9781 cagggtaata agattatcat tttaaacaag tgttccatca caaacctcct cttttaaatt
     9841 attttcttta gtttcgttct attaaatgtt attaccaata taaaccttgg aatagcgtgt
     9901 aataaatgat tttagaggta ccataagcca cctcctacaa atagaaaccg atataaatca
     9961 atgccttcca cccttagact tccctagttc ctgtctcaag cgaaacattt ctttgaaaca
    10021 ggaataagtt aaccaattca taccaatagc tagcagaata aaaagaaacc aaatgcccca
    10081 taacttgata tctgtcacat ttctcaagac ggtattgaaa aacagaactg aaacaactgt
    10141 ccaagcaagg ctaaaaagag aatagaaggg gatgtaaaac cagtaaaaat aataaaaaat
    10201 tggaaaaaac ttactatttc tgttggcctt ttcaatccag ttatcaaaat aaaagtacgg
    10261 tgctaaaagt aagaatttaa acaaatgttc catcaccgac atcccccctt cttttgatag
    10321 cgttttctat tattttatta tatcaaaaaa atccggaact gtcattccag attctacttt
    10381 tttatttgcg ttttcttgcg atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc
    10441 ggatttgatt ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct tcattgacaa
    10501 agatgacaaa ggttggtggt ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt
    10561 ttcctttgtc tgtcggtgtt gggttgatgg caatggcatc catgatgaca tcgttcaaga
    10621 cagctgatgg aatacgtgta ttttgacttt cgctgatttg cttaatcatc tcaggaagtt
    10681 tgtggagacg ttgcttggtt aaagctgata caaagataat cggtgcgtaa ggcaggtatt
    10741 ggaactgctc acggatatct tcttcccagt ttttcatagt gtggttatct ttttcaagcg
    10801 tatcccactt gttgaccacg ataatcatcc ctttaccagc ttcatgggca aatcctgcga
    10861 tacgcttgtc gtactcacga atgccttctt ccgcattgat gaccatcaag accacatctg
    10921 aacggtcaat agcacgcatg gcacgcataa cagagtattt ctcagtattt tcataaacct
    10981 taccagactt acgcatacca gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg
    11041 taaagtgggt atcaatggca tcacgagttg ttccagcaac aggactagca ataacacggt
    11101 cttctcccaa gatagcattg atcaagcttg attttccaac gttaggacga ccaatcaagc
    11161 taaacttaat gacatctgga ttttcttcct catattcatt tggaagattt tctacgatcg
    11221 catctagcac atcccctgta ccgattccat ggacagatga gataggcaat ggttcaccca
    11281 aaccgagagc atagaaatca tatatatcat ttctcatctc agggttgtcc accttgttga
    11341 ctgcgaggat aactggtttg tgggtcttat aaagcttacg agctacgtat tcgtctgcat
    11401 cagtaattcc ttccttacca gacacgacaa aaacgataac atctgcttct tccatggcaa
    11461 tttctgcctg gtgcttgatt tgttccatga aaggagcatc gacatcatca attcctcctg
    11521 tatcaatcat gctaaaagaa cgattgagcc actcacccgt tgcataaata cggtcacgtg
    11581 tcactccttc gacatcttct acaatggaga ttcgctcacc agcgatccga ttaaataggg
    11641 ttgatttccc aacattggga cgtcctacaa tggcaatagt tggtagggcc ataatttctc
    11701 actttctaca ataatttctt ctgttcaaga ttttttctag ttgagcttgg ttcagcttga
    11761 ccaaactgtt ctgctaggcg ctgactccag cttgtggtcg cacgcgcccc agcatagtca
    11821 gcctgaacac ggtcataagc ttggattgcc tcagttgact gttcttggta ttcttcctca
    11881 aagacaacat tctctagtgg cagtctcggt ttcatatcat gatgttgatt tggcacaccc
    11941 agtgccatcc caaagacaga ataggtgtag tcaggtaggt taaagagctc tgccacttct
    12001 tcagacttgt atcgaaccaa accgataatc acaccaccat agcccaagct ttcagctgcc
    12061 aacaaggcgt tttgtccagc aagagctgca tcgaccgaac taatcaagag accttccaca
    12121 ccttggggtt ggaaggtgtc ggtatgaagt cgggctccct tttctgctcg gttcaaatct
    12181 ccgacaaaga gaaggaaaac agcagactgg cgaatggctt cttgaggtac caattcatac
    12241 aaggcatctt tcttctcttg acttcgtacc acaatcacag agtaggattg gaaattcttc
    12301 caagatgatg ccatctgggc tgctgtcaaa atctcattta agtctacttg gggaatttct
    12361 tgctctttaa acctgcgcac tgaagtatga gccttcatca atttaatggt ttctgtcatc
    12421 gacggtttac tccttctaaa cgagtctcct cagccaaata acggatgcgt tccatgaccc
    12481 gtctggcttc ccaggtttcg tcatttccat gtttcacttt cgcaaaatgc ttctccaaat
    12541 cttcaaagtt gaagttggat gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga
    12601 gaatgacctg caggatttcg tcacgcaccc aaacggttga ttgctcggcg ccaatatcat
    12661 ctaaaatcag gacctcagac agcttaatct catccaccaa ggtcttaaca ttgccatcac
    12721 tgatagcatt tttgacatca atgacaaagc taggatagtg gaggagagtt gatgaaacac
    12781 cacgtttttc tgataaatca tgagctaagg ccgccaccat gaaactttta cccacaccaa
    12841 agtctccata taagtaaaga ccttttcgaa tagctggata ttgctccacg aaggctagta
    12901 gcttttcaaa aactggtaag cgccccaaat catccaagtc aacttgagcc aaactagctt
    12961 tcttgagact ggctggtaga ttgattaact tgagacggtt cttaatagcc gcttcttttt
    13021 cagccgcgat tagctcagga gtttcttcat atgaaacatc tgcataacca tgattcttaa
    13081 ccaaaatcgg cttgtagcct ttggcaatat aatccgtatc cccacggaga aacttgtcac
    13141 gctcggtgat gtactgatta aacttggaga tactgcgatt taattccttt ggagttaagg
    13201 attcttgctg gataaaggcc gcaacatcag ggtccttcat gattttctgg accaaatctt
    13261 gataataaaa acggctgggt tgacgtttga gtacgtctcc gacactttcc atctaatctc
    13321 ctcctttttc taatcgagct aatagttctt gcttcttacg ttctagttcc agacgagttt
    13381 cctcgctggt ttcattctta tattcaggat tactccattt aggaacattg gttttttctg
    13441 gggcagtctg attctgtttt tgtgtttttg ctttctgccc tcgatcacga attcgtaaaa
    13501 cggcctcttc tgccgaatga atcttttgat aggcatagtc attggctacc ttcatggcat
    13561 atttctcatt gatatttgcc gaatccacct tattaaaggt caataagaga ataatattga
    13621 tgacttcgtc cagtaagccc aagccagcca tctgttgcaa gagttctctt tctgtttggg
    13681 taatggttcc cttgcgtgtt tgcttgattt ctgctaagaa ctgcagggca gttttacttt
    13741 tagcttcttt gataatggtc gcttccttaa gactaaagtc agaggaaact ggtttttgag
    13801 caattttttc acgcatgcgt ttggttgaaa taacctggga aacagctgtt gacttggcca
    13861 attgataggt ttcaaaccaa gtccatttct tctcctcggc aatagcaaag aggtttaaga
    13921 catcggactg ctcatccgca aaacgaagtc catctcgagc catcagctgg cgaaaatgtt
    13981 ccaagtcaaa atcattggcc actttcttct tgagaccaag gtcttcttga ctgcctagtt
    14041 ctgccaattc tggaaagact tgattgagtg agacaggtat ttcttcacca tcagcacttt
    14101 caactttcaa atcctccaca gctacatcgc caatcttttt ctctaagagt ctgcgataaa
    14161 caggatgccc caagaagtct tgactagata gaggagcatg gagggctagc tgataaacat
    14221 cacccttttg atagagggtc aagagattaa aagcagataa gattttcaat gattttatca
    14281 gtctatccat cccaaagttg agatggttga gaatgcttga aaaaagatat tcctttctac
    14341 cattatccca aaaactgatt gtataaagat aaaggctcag tgcctcctga ccgataatcg
    14401 ggaggtagca ctgtaccaga gatgaggtat cttgcgacac ccgattattc tttagataag
    14461 aaaaacggtc aattggcttc atttatcttt cctttttctt tttagaggac tgggtgattt
    14521 gttggagcaa gctctctaac tcactgacat ccttaaaact acgatagaca ctagcaaaac
    14581 gtacataggt aatctcgtcc aattcagcca actcctccat gacgagtgaa ccaatgtcct
    14641 cactttgaat ttcattttca tttcgaccac ggagtttctg ttcgatacga ttgactacca
    14701 tgttgatttc atcacttgac acaggacgtt tctgggctga gcggataatc ccattaaaga
    14761 ttttatctct ggagaattgt tcccgtgtgc catctttttt aacaaccact aaggttcttt
    14821 cttctactcg ttcgtaggtt gtaaaacggt gttggcattc gtcgcactca cgtcttctac
    14881 gaatggtgtt cccttcttct gcttggcgac tatcgataac acttgacttg gtagccccac
    14941 attttggaca acgcatcctt tccctcctta tcgttttctt ttcattatac cattttttaa
    15001 gcgattccca aaacaattct tctttttgct tgacaagttt tttgttttgt tgtattattt
    15061 aattaagaca acaaggtaaa agaaaggaga ctaagatgtc ctggacattt gacaacaaaa
    15121 aacccatcta tttacagatt atggagaaaa tcaagcttca gattgtttcc catacactgg
    15181 aacccaatca acaacttcca accgtgaggg agctagctag cgaggctggt gtcaatccca
    15241 ataccatcca aagagcctta tcagaccttg aacgagaagg atttgtctac agcaagcgaa
    15301 caactggacg atttgtgact aaggataagg agctaatcgc ccagtcacgc aaacaattat
    15361 cagaagaaga attggaacac ttcgtttcct ccatgaccca ttttggctat gaaaaagaag
    15421 aactaccagg cgtagtcagt gattatatta aaggagttta agcctatgtc attactagta
    15481 tttgaaaatg tatccaaatc atatggagca acaccagccc ttgaaaatgt ttctcttgac
    15541 attccagctg gaaaaattgt cggccttctt gggccaaacg gctcaggaaa aacaaccctg
    15601 attaaactaa ttaatggcct cttacaacca gatcaaggac gtgtcctcat caacgacatg
    15661 gacccaagcc cagcaaccaa ggccgttgta gcttatttgc ctgatacgac ctatctcaat
    15721 gagcaaatga aggtcaaaga agccctaacc tacttcaaga ccttctataa agatttcaat
    15781 cttgaacgcg cccatcatct acttgcagac ctgggcattg atgaaaatag tcgtctcaag
    15841 aaactatcaa aaggaaacaa agaaaaggtt caactgattt tggttatgag ccgtgatgct
    15901 cgtctctatg ttttggacga acccattggt ggggtggatc cagcagcccg tgcttatatc
    15961 ctcaatacca ttatcaacaa ctactcacca acttctaccg ttttgatttc tacccacttg
    16021 atttctgata tcgagccaat cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc
    16081 cgtcaaggaa atgtagatga tattcgctac gagtcaggtg aatccattga ccaactcttc
    16141 cgtcaggaat ttaaggccta agcaaaggag attatttatg ttttggaatt tagttcgcta
    16201 cgaatttaaa aatgttaaca agtggtattt agccctctac gcagccgtgc tagtcctttc
    16261 tgccctcatc ggaatacaga cacaaggctt taaaaatcta ccttaccaag aaagtcaggc
    16321 tactatgcta ctttttctag ctacagtctt tggtggcttg atgcttacac ttgggatttc
    16381 aaccattttc ttgattatta aacgcttcaa aagtagtgtc tacgaccgac aaggctatct
    16441 gactttgacc ttgccagttt ctgaacacca tatcatcaca gccaaactaa tcggtgcctt
    16501 tatctggtca ttgattagca ccgctgtatt ggctctaagt gctgttatta ttctggcttt
    16561 aacagctcca gaatggattc ctctttctta tgtgattaca tttgtagaaa cacatctccc
    16621 tcagatcttt cttacaggta tatccttcct actaaatact atttcaggaa tcctctgcat
    16681 ctacctggct atttccattg gacagctttt caatgaatac cgtacagcac tcgctgttgc
    16741 agtctacatt ggtatccaaa tcgtcattgg atttattgaa cttttcttca atcttagttc
    16801 taatttctat gtcaattcac tggtaggact caatgaccat ttctatatgg gagcaggtat
    16861 agccattgtt gaagaactca tattcatagc tatcttttat ctcggaacct actacatctt
    16921 gagaaataag gttaatttgc tttaaataat ttttacctag atatgtaaca tactcataga
    16981 acaaaagaga ccaggcaaaa agtctttaaa attagaaaac gcatagtatc aggtgttgaa
    17041 tatgtactgc cccccaaaag ttagattttt tctgtctaac ttttgggggc agttcataag
    17101 aaccttggta atatgcgttt tttgtgagct gacttatttc ctttcactat atcgcaaaat
    17161 gaaataagaa cggaacgatg ggattttgga attcaaatca atttataaga atgttttaga
    17221 agtaatatta tcctattcca gattcagttc actatacaat tgagttttca agcaacctgt
    17281 ttacataatg tgtacataat taggttcgtg attccaccct tttcaccttt aaaaacctcg
    17341 ctttcgcaag gctcttctat ttataagata aggcacgttt aaaggttttc caaatcccta
    17401 aatcatccgt ttgaagaacg agactagcat acatgcgtcc gataaatcct gttgctacca
    17461 ccgcaaaaat cactgtaata gcaagtgaaa tccatgcttc tgctcccccc gcatagtcat
    17521 taatcgttcg aaacggcata aagaaggtcg aaataaaggg aatataagaa ccaatcttca
    17581 agaggagatt gtcaccagct gcacctagag ctgtcactcc aaaaaaacca cccataatca
    17641 aaatcatcaa aggcgacaag gctttccctg agtcctcagg acgagaaacc atagatccta
    17701 ggaaggctgc caagactacg tacatgaaaa gactgatcaa aataaagagc aaggtattca
    17761 gtgagatagc atctcccaag tgatccaaaa taccagactg agccaagaat ggcaaatctt
    17821 taaagagcaa aacggcagcc agaccaccta caacatagat cccaatatgc gttaaaatca
    17881 ctagaaacag agccatcatc cgcgcataga aatagtgact tgcccttatg ctagaaaaaa
    17941 cgacttccat aattttggtg cctttttcac tggcaacttc ctgagctgtt acacccgcat
    18001 aggtaatcag aatcatataa agaaagaatc ctaaggcacc tgctgcaatt gtttgaataa
    18061 actttttatt ttccttggct tcatcaatct tttctgtgaa ttgaattgtc tgcgctaagc
    18121 gtttttcctg ctcttgagac aaggaagcag ttgaacgatt aagctgattt tgcagttcat
    18181 tgagtgtacc tgtaacctca aatttaattc cattttcaag cgatgtttcg ccatgataaa
    18241 ctgcctttag aacactatct tcttgatcaa tggtcaaata accttttaat ttttcttctt
    18301 taattgcttc tttggcactt gcttcgtctt tatagtcgaa gttaacacca tttacattct
    18361 tcagtccttc tgctacagat ggcactgttg tcactactgc cactttatta tttttagcca
    18421 tagaagaacc ttggagatgc ccaattccta cagagattcc taaaaagagg aacggcgaaa
    18481 tcaccataaa gaagaaactc catgactcga catgtcgaag ataggtttcc ttgattacaa
    18541 cccacatatt tctcatactt ccactcctga ttctagttta aagatttcat cgatagttgg
    18601 cgcttgttgg tcaaatgttg cgatatattg accttgagtc aagattgaga agagttccct
    18661 tccagcgctc tcatcctcca aaatcaattt ccaactgcct tgtttggtca agctcacctg
    18721 tttgacatga ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa caaagagacg
    18781 cgttttcccg tattgattgc ggacatcctg aactggtccg tgcaagacca cacggccatc
    18841 tcggatcatc agaatatcgt cacaaagttc ctcaacattg gtcatgacat ggtcagaaaa
    18901 gataatggtt gctccgcgct ctttttcctg aaaaatgact tgtttgagca attctgtatt
    18961 aactgggtcc aatccactaa aaggctcatc caagataatc aggtctggtt catgaatcag
    19021 agtaataatg agctgaatct tctgctgatt tccttttgac agactcttga ttttatctgt
    19081 cagctttcct ttcacttcca acctcttcat ccattgaggg agtttttctt tgacttcttt
    19141 ggcatccatg ccttttagag tcgccaagta gcgaacttgt tcaagaactg tcaatttagg
    19201 catgagactg cgttcttcag gcagataacc aatccgagca taggtctcct gacgaatatc
    19261 ctgaccatcc agaccgattt ctccctgata ttctaggaat ttcaaaatac tatggaaaat
    19321 cgttgttttt ccagcaccat tttttccgac tagtcccaaa atacgacctg gtcgcgcttg
    19381 aaagtcaata ccaaacaaaa cttgcttgga tccaaaactt ttctctagac ttcttacttc
    19441 tagcatcttt cacctccgaa atttcttgca ctcattatac tcctttttga tagcctttac
    19501 aatgtttttt gtccattttt agaagactat tgctgtgtaa aatatggcct ggagcacttt
    19561 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgtagactgc tcaaagtaca
    19621 gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtgg
    19681 atagaactga cgaagtcagt aactatatct acggcaaggc gaagctgacg tggtttgaag
    19741 agattttcga agagtattag tgataaatcc attatacagc agcaaactta atttatacct
    19801 tccgctcctc aactgtctat ttttaatcct gaattgttat ttgagtaact cctttttcct
    19861 cgtaaagttt tcttcctcta aaacttctgg aaaaaggcta atagtttcag acaacatttt
    19921 tataagaaac aagttcatct gtcatttcaa gaaggagtaa tcctttatct actaatggac
    19981 ggaacagaat tcaaccgctt gtccgatatg ttttctaagg attatatagt aaaatgaaat
    20041 aagaacagga caaattgatc aggacagtca aattgatttc taacaatgtt ttagaagtag
    20101 atgtatacta ttctagtttc aatctgctat atctattatg cacaccccta taggatctaa
    20161 tgaaaatcac aacaggctca ttcatagatg gttacctaag cctaagggaa ctaagaaaac
    20221 gactaccaag gaagtcgcat tcatcgaaaa gtagattaac aactatccta aaaaatgctt
    20281 gaactacaag tcccccagag aagacttctg gatgactaac ttgaacttga aatttagcaa
    20341 taattaattc actatctaac tatatttagt aattatttca gaactgatta atattaaaat
    20401 taactaacaa ttcaaaggat tcatactagc cataaattac gtccatcaga gagagactct
    20461 tactactttt agattttagt ctttctagct tcagaataca tctaaacttt agggaaaatg
    20521 actattcgaa agcgcgaatg cctcaaaatt atctcagata agctattcga aacttagaat
    20581 gcttttaaat ttatggaatt gcgattattc gaaacctaga atgcatataa cctttagttg
    20641 acagacctat tctaagtctc gaagggctat ttactttcta ttccttatca aaaaagactc
    20701 attccccctt tctcctccaa aatatggtat agtagaaata tactatctat gaggagttta
    20761 catgtcacag gataaacaaa tgaaagctgt ttctcccctt ctgcagcgag ttatcaatat
    20821 ctcatcgatt gtcggtgggg ttgggagttt gattttctgt atttgggctt atcaggctgg
    20881 gattttacaa tccaaggaaa ccctctctgc ctttatccag caggcaggca tctggggtcc
    20941 acctctcttt atctttttac agattttaca gactgtcgtc cctatcattc caggggcctt
    21001 gacctcggtg gctggggtct ttatctacgg gcacatcatc gggactatct acaactatat
    21061 cggcatcgtg attggctgtg ccattatctt ttatctagtg cgcctatacg gagctgcctt
    21121 tgtccagtct gtcgtcagca agcgcaccta cgacaagtac atcggctggc tagataaggg
    21181 caatcgtttt gaccgcttct ttatttttat gatgatttgg cccattagcc cagctgactt
    21241 tctctgtatg ctggctgccc tgaccaagat gagcttcaag cgctacatga ccatcatcat
    21301 tctgaccaaa ccctttaccc tcgtggttta tacctacggt ctgacctata ttattgactt
    21361 tttctggcaa atgctttgac acgtaaaaaa tccgtttggt ttcccaagtg gatttttaaa
    21421 gcgtagatta actatagctt gatactaaat atactttggt atggaaatca tgcatatttt
    21481 tcgatagtga ggcgaggact tacctagcct ttccgccgtg atagaaacac ctgaaatcta
    21541 atggtttcag gtattcggaa actttgagcc tagtgtctca aagtttaggt atggaatttt
    21601 gaagaaagtc gctaccgtcc gtaatcactt aaggaaaggc tcaaaaatat tgttttcaac
    21661 cacaaaatcc gtttggtttc ccaagcggat tttgtgcttt attttgaaac ttcttttgca
    21721 agaacaaagt tcccaagtgt ggcagaacca tttcctgcga ctgctggcgt cacgatatag
    21781 tcacgcacat ctggtactgg taggtaacca ttaagaagag atgtaaattt ctcacggaca
    21841 cggtccagca tatgttgttg agccatgacc cctccaccaa agacaatcac gtctgggcgg
    21901 aaagtcactg tcgcattaac cgcagcttga gcgatatagt aggcttgaac atcccaaaca
    21961 gggttgttga gttcaatagt ttccccacgt acacctgtac gagcttccaa acttggacca
    22021 gctgcataac cttctagaca tcccttatgg aaaggacaaa cacccttaaa ctctttttca
    22081 atatccattg ggtgtctagc aacataataa tgacccattt cagggtgacc cacaccaccg
    22141 ataaactcac cacgttggat gacgcctgca ccgatacctg taccgattgt gtagtaaacc
    22201 aagttttcga tacgaccacc agcattgtta cgggcaacca tttcaccgta agcagagctg
    22261 tttacgtctg ttgtgaagta cattggcacg tttagggcgc gacgaagggc accaagcaag
    22321 tctacatttg cccagtttgg ttttggagtc gtcgtgataa agccataagt ttttgagttt
    22381 ttgtcaatat caatcggccc aaatgaacca actgcaagac cagcaaggtt atcgaatttt
    22441 gagaagaact caatggtttt atcgattgtt tcgattggag ttgttgttgg aaattgtgtt
    22501 ttttctacaa cgttaaagtt ttcatcaccg acagcacaga caaactttgt accgcccgct
    22561 tccaagcttc catataattt tgtcatgata aacctcttgt ttttattttc tttattatag
    22621 catacttcga aagtctaaat gtctctattt tttagatttt cctctgtaaa tcttactatc
    22681 taataaaaac gaacaaacat gtcatttgtt cgttttcaca ttagagagga ttgattagat
    22741 tttcacttcg atcacagcat cccccttagc aactgaacct gttgcgactg gagctactga
    22801 agcgtagtca cctgtatttg taacgataac cattgttgta tcatcaagtc cagctgcagc
    22861 gattttgttt gagtcaaatg ttccaagaac atcgccagct ttcaccttat taccttgagc
    22921 aacttttgtt tcaaaaccgt caccgttcat agatacagta tcaataccaa catgaatcaa
    22981 aacttcagca ccatttcttg ttttcaaacc aaaagcgtgc cctgttggaa aggcaattga
    23041 aacttcagca tcagctggtg catagaccac gccttggctt ggtttcacaa cgataccttg
    23101 tcccatagct ccacttgaga agactgggtc attgacatca gcaagagcga caacatcacc
    23161 gacgatagga gttacaagtg tttcattttg aagagctgct ggcgcaactt cttctttttc
    23221 ttcagccact tcagctcgtt ttgcagctgc agttgcgtct acttcatctt cgtaaccaaa
    23281 catgtaagta agagcaaaac caagggcaaa tgatacagct accataagaa ggtattgtgg
    23341 aagttgtccg ttaccaacat aaagcattgt accagggatg atggtgatac cattaccagt
    23401 accagcaagt ccaaggatag aagccaatcc accaccgatt gcaccagcaa tcaatgaaag
    23461 gaagaatggt ttacggaagc gcaagttcac cccgaagata gcaggctctg taatacctag
    23521 gaaggcagaa agagcagccg ggaaagcaag tgttttcagt tttggatttt ttgttttaac
    23581 accaaccgca acagtagcag caccttgagc tgtcatagca gctgtgatga tagcgttgaa
    23641 tgggttagca tggtcagcag caagtaattg cacttcaagc aagttgaaga tgtggtgcac
    23701 acctgacacg acgatcaatt ggtgaacccc accaatcaag aaaccaccaa gaccaaatgg
    23761 catgctaaga atcgcttttg tagcaataag gatgtagttt tcaacaacgt ggaaaactgg
    23821 tccaatgaca aagagtccaa ggatagacat gaccaaaagt gtcacgaatg gtgttaccaa
    23881 gaggtcaatg acatctggaa caaccttgcg gacagctttt tcaaatttag ctccgacaac
    23941 cccgatgatg aaggctggaa gaacggaacc ttgcaaacca acaacaggga tgaaaccaaa
    24001 gaagttcatc gctgttactt caccaccttg agcaactgcc caagcgtttg gaagtgagcc
    24061 agagacaagc atcataccaa gaacgatacc aacggcagga tttccaccaa atacacggaa
    24121 ggttgaccac acaaccaaac ctggcaagat gatgaaggct gtatctgtca agatttgtgt
    24181 gtaagttgca aagtcacctg gaagtggcat ttcaagagcg ttgaaaagac cacgcacacc
    24241 catgaagaga cctgtcgcta cgataactgg gatgattgga acgaaaacat caccaaaagt
    24301 acggatagca cgttggaacc agttcccttg tttagcaact tctgctttca tgtcatcctt
    24361 agatgatgtt ggtaatccaa gtacaacaac ttcatcgtac attttgttaa ctgtacctgt
    24421 accaaagata atttggtatt gccctgagtt aaagaaagca ccttgaactt tttccaagtt
    24481 ctcaatcact tctttattga ttttctcttc atctttgacc atgacacgta gacgagtcgc
    24541 acagtgggca acactattga cattttcacg tccgcccaag gcatcgatga ctttttttgc
    24601 aatttcctga ttgttcattt gcaaaaatct ccttatataa cattttgttc ttgtttgaaa
    24661 gcgattttat tcgcccttac gactattatt ttatcatgtt ttaaaaatat gtcaagcgtt
    24721 ttgcagaaaa aaattctatc cacttagtgg gcaagcttta gattgattgt ttttgactgt
    24781 ttcatcagga attccttgaa aaataagttt gaaaccttgg ttaaaaagta ttttatttca
    24841 ttatctttca cattttcgta aaaatttgat tgatttcctg tatttatttt atgataatcc
    24901 tttgacagtt tttcctcttt tttaacataa aaggcttgca ttttttatcg aaaacagtta
    24961 ctattaaaag tgataagatt tttggaggaa tgaatggaat ggacaactga gcgtcgttac
    25021 agactttatc aagattggac gcaagaagaa attcaacata taaaggaaaa tatggcacaa
    25081 tctccatggc atactcatta ccatgttgag ccaaaaacag gacttctcaa cgacccaaat
    25141 ggcttttctt actttgatgg caagtggatc ctcttttacc agaattttcc ttttggtgca
    25201 gcccacggtt taaaatcttg ggcacagcta gaaagtgatg atttgattca ctttaaagaa
    25261 actggaatca aagttttacc agatactcca ttagatagcc acggtgccta ctctggttct
    25321 gccatgcaat ttggcgataa cttattccta ttttatacag gaaatgttcg cgataaaaac
    25381 tggatccgtc acccatacca gatcggtgct ttgatggaca aggagggtaa gattacaaag
    25441 attgacaaga tcttgattga ccagccagca gactctactg accacttccg cgatccacaa
    25501 atttttaact ttcagggtca atattatgcc attgtcggcg gacaagactt ggagaaaaaa
    25561 ggtttcgttc gtctctacaa ggctgtcaat aacgactaca caaactggca agcagttggc
    25621 gaccttgact ttgctaacga ccgtactgcc tacatgatgg aatgtcctaa tttggtcttt
    25681 gtagaggaac aacctgtcct tctctactgt ccacaaggat tggataagaa agttctagac
    25741 tacgataata tctttccaaa tatgtataag atcggggctt cctttgaccc taaaaatgcc
    25801 aaaatggtag atgtgtctca acttcaaaac atggattacg gtttcgaagc ctatgcaact
    25861 caagccttca acgctcctga tgggcgtgct ctagcagtta gctggcttgg tttgccagat
    25921 gtttcttacc catctgaccg ttttgaccac caaggaacct tctctttggt caaggaactc
    25981 actatcaaag acgacaagct ctaccagtat ccagtcgctg ctattaagga ccttcgtgct
    26041 tctgaagaag ccttctcaaa ccgttcccaa accaagaaca cttacgaact tgaactcaac
    26101 ttggaagcta atagccagag cgagattgtc ttacttgctg ataaagaagg taagggactt
    26161 tcaatcaact ttgaccttgt aaacggtcaa gtaacagtgg atcgtagcca ggctggagaa
    26221 cagtatgccc aagaatttgg gacaactcgt tcttgcccta tcgagaatca ggctactact
    26281 gctacaatct tcatcgataa ctctgtcttt gaaattttca tcaataaagg agaaaaagta
    26341 ttttctggtc gtgtcttccc acatgcggac caaaatggta tcctgattaa atctggaaac
    26401 ccaactggaa cttactatga attagattat ggtcgcaaaa ctaactgatg tcgccaaact
    26461 tgcaggcgtc agtcctacta ccgtttctcg ggttatcaat aaaaaagggt atctatctga
    26521 gaaaaccatc caaaaagtca atgaagccat gcgagaattg ggctataaac ccaacaacct
    26581 ggctcgtagt ctgcaaggaa aatcagctaa gttaatcggc ttgattttcc ccaatatttc
    26641 caatgttttc tatgcagaat tgattgataa attggaacac caactcttca aaaatggtta
    26701 caagaccatc atctgcaaca gtgaacatga ttctgagaag gaacgcgaat acatcgaaat
    26761 gttggaagcc aatcaggtgg acggcatcat ttctggtagt cacaacctag gaatcgaaga
    26821 ctacaatcgt gtgacagcgc cgattatttc ctttgaccga aacctatcgc cagacatccc
    26881 tgtcgtctcc tctgacaact atgctggtgg ggttcttgct gcccaaacct tggtcaagac
    26941 aggtgcccag tctatcatca tgattacagg gaatgacaat tctaattcgc caaccggact
    27001 gcgccacgct ggttttgcat ccgtactccc aaaagctcct attatcaatg tttccagtga
    27061 cttttctccc gtcagaaaag aaatggaaat caagaatatc ttgacccggg aaaaaccaga
    27121 tgccattttt gcttcggatg atttgacagc tattctggtc attaaaatcg ctcaagaatt
    27181 gggcatttct gtcccaaaag agctcaaggt catcggctat gatgggacct actttatcga
    27241 aaattactac cctcaattgg ctactatcaa gcaacctttg gaagagattg cttgtctcac
    27301 tattgatctt ctcttgcaaa agattgaagg caaggaagtc gccacaactg gttacttctt
    27361 accagttacg ctattaccag gaaaaagtat ttaaacacaa gaaaactcag accgattcgt
    27421 ctgagttttt atgatcttaa attttcgaga tagcgctggg ctgtctctag gttaaaggtt
    27481 ttatctgaga tgaggcgctc tactagggga gcaacttcag attcactagc cccagctagg
    27541 agagctaggg atttggcctg tagtttcatg tggccttgct ggatgcccgt acttaccaag
    27601 gctttgaggg ctgcaaaatt ttgagcaaga ccgatggaca cgataatctg ggctaattct
    27661 ctggcagaag gatttcctag tagatcatga ctgagaacta cacgtgggtt gaggccgata
    27721 gagccaccct tagtcgctac aggcatgggc agggtcatct caccgaccaa ttcttctctt
    27781 tcaaggtcca gcgtccagca gctaagacct tgatagcgtc catctcgact ggcaaaggca
    27841 tgggccccag cttcgatggc acgccagtca ttaccagtgg caatcaaaat cgcatcaata
    27901 ccattaaaaa ttcctttatt atgagtagca gctcggtaag gatcagcctg cgcaaactga
    27961 ctagccaacg caattttctc cgcaatctct cgtccttgat ccttttggcg gctcaagtag
    28021 cgaaaggcga tgcgacagct tgcagtcacc agagaatcgg tcgcgtagtt ggacaggatt
    28081 cccatgagac tctgtccctg actgagttct tctaagactg gtttcaaggc ttccagcatg
    28141 gtgttgagca tattggcacc catggcttcc tgggtatcga catgaatata aacaacgaga
    28201 aagtctggtt cgccttttat ctgctcgaca tgcagatcac gcgccccacc tccacgttta
    28261 acgatagaag gataggcttg attggcaagc tccaagagct ccgctttctt gctggcaatt
    28321 ttctcttgcg ctagtttagg attagcaact tgataaaggg ctacctgccc aatcatctgt
    28381 cgctgatgga cttgtgcagt aaaaccacct gcacgcttga tgattttgct ggcatagctg
    28441 gccgccgcaa ccacagaggg ttcttctgtc acatagggaa cggtgtattc ctgaccgttg
    28501 acaagtacct ccggaaccag tgaataaggc agagaaaaag ttcccactac attctcactc
    28561 agctggtctg ccacagtcac gctcatctgt tcatccttct ccagactagc ttgtctctca
    28621 ggactaagga gcgcctgagc ttttaacagc tcgaggcgct cttggtatga ttttttagaa
    28681 aatccattcc aacttatctt cattattttt caaccttgct ataacggcgt tggtggtcga
    28741 gaatttcaac caaggcaaaa tcttgatttt catagccagc aaactgggca gagttagttt
    28801 catccaagtt tacttcctca aaaaagacct tttcatagtc tgcaacggat agggcagttc
    28861 gttggttgag cttgttcaaa cggtctttat ccaaataagc ttcatatcct tcaaccaatt
    28921 caccactgaa gaactcagcc acagctccac ttccgtaact ataaagggcg attttatccc
    28981 cagctttcaa gctatctgta ttttccaaga gagacaaaag tccaaggaaa agtgaacctg
    29041 tgtagatatt ccccaccttt tgactgtaga gaatagactg gtcaaaatgc ttttgtaaga
    29101 ggtctttttt ctcttgaggc aggctcttat ccatgatttt tttcaagcct tttagcgcta
    29161 atttaggata aggcaagtgg aaacaaacag ccgcaaaatc atccaaagta agctggtagc
    29221 gtttttgata ttcaagccaa gtcgttttca aactatccaa gtattgttgg gtagaataga
    29281 caccatttac ataaggagtt gtcgagtaat ttggtcgcca gaaatccatg atgtcacggg
    29341 tctgagctac attgtcatta ttaaaggcca tcatgcgtgg attttgtgta atcaacatag
    29401 ctacacttcc agcaccttga gttggttctc ctggagtttc aataccgtat ttggcaatat
    29461 cactggcaat gaccaagacc ttggactccg gagaattttc cacatgcaat ttggcataat
    29521 ggagggcagc agtcgctccg tagcaggctt ctttaatctc gaaactacga gcaaagggct
    29581 ggatgcccag caagccatgc acaaagacgg ccgcagcctt actctggtca attcctgact
    29641 cggtcgccac aatgaccatg tcaacttctt gtctttcttg ctcagttaaa atagagtcac
    29701 tagcactggc cgccaaggtc acgatatcct cagttagggg cgcaatactc aattccttga
    29761 gtaagagtcc tttacttaat ttttcagggt caattcccct cgcttctgct aagtcttgta
    29821 atttcaagac atattgactg gtcgcaaaac caatcttatc aataccgatt gtcatattta
    29881 cctctgtttt atcattcatg taaaaaatcg ttctatacta ttttatcaca aatggcagta
    29941 aaagagagaa aaaagacttg attcaccaaa tcaagcctct tattggtcat cattttaaag
    30001 aatgattagt tgctagagag ttcaccgata taagtagctt tataagctcc attcacagtt
    30061 atcagctcct ggaggatcaa atttcctgag taagtccttc ccatctcatc tacaaatttt
    30121 tgataaaact gactggtcgg aatttctctg acatccttat caaatgtctt atcaagtgtt
    30181 ttactaacct tctcagcaat caattgatgc tcttgccatc cactttgaaa ctctgagccc
    30241 gaactagaaa ccatgactgg gataaacaac aaggtcagta gatttacaga caataaggaa
    30301 agtagtagac ttcctgcaaa actagaatcc tagttcatga ttgataatac cagcaatcaa
    30361 attcattcgt aatccgaagc gtttacgatg atttcgatag gttgttgaaa acattttaaa
    30421 cgtttttact ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt
    30481 acaggcttta tcttcagctg ttagcggctt gagtttgctg gatttacgtg gagtttgtgc
    30541 ttgaggatat atcttc
//
LOCUS       DBDESG010000024        30280 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.024, whole genome shotgun sequence.
ACCESSION   DBDESG010000024 DBDESG010000000
VERSION     DBDESG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.024"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1704)
                     /gene="spuA"
                     /locus_tag="ACKB38_001029"
     CDS             complement(<1..1704)
                     /gene="spuA"
                     /locus_tag="ACKB38_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGL7979810.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AK"
     gene            2121..2534
                     /gene="rpsL"
                     /locus_tag="ACKB38_001030"
     CDS             2121..2534
                     /gene="rpsL"
                     /locus_tag="ACKB38_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGL7979811.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            2554..3024
                     /gene="rpsG"
                     /locus_tag="ACKB38_001031"
     CDS             2554..3024
                     /gene="rpsG"
                     /locus_tag="ACKB38_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGL7979812.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            3449..5530
                     /gene="fusA"
                     /locus_tag="ACKB38_001032"
     CDS             3449..5530
                     /gene="fusA"
                     /locus_tag="ACKB38_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGL7979813.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            5634..10025
                     /locus_tag="ACKB38_001033"
     CDS             5634..10025
                     /locus_tag="ACKB38_001033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGL7979814.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            10127..10390
                     /locus_tag="ACKB38_001034"
     CDS             10127..10390
                     /locus_tag="ACKB38_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGL7979815.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            10383..10661
                     /locus_tag="ACKB38_001035"
     CDS             10383..10661
                     /locus_tag="ACKB38_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGL7979816.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARN
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            10682..10834
                     /locus_tag="ACKB38_001036"
     CDS             10682..10834
                     /locus_tag="ACKB38_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979817.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            10856..12097
                     /locus_tag="ACKB38_001037"
     CDS             10856..12097
                     /locus_tag="ACKB38_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGL7979818.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            12107..12337
                     /locus_tag="ACKB38_001038"
     CDS             12107..12337
                     /locus_tag="ACKB38_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGL7979819.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            12358..12516
                     /locus_tag="ACKB38_001039"
     CDS             12358..12516
                     /locus_tag="ACKB38_001039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979820.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            12605..13327
                     /locus_tag="ACKB38_001040"
     CDS             12605..13327
                     /locus_tag="ACKB38_001040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGL7979821.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            13545..14879
                     /gene="pepC"
                     /locus_tag="ACKB38_001041"
     CDS             13545..14879
                     /gene="pepC"
                     /locus_tag="ACKB38_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGL7979822.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(14935..15846)
                     /locus_tag="ACKB38_001042"
     CDS             complement(14935..15846)
                     /locus_tag="ACKB38_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGL7979823.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(15870..16673)
                     /locus_tag="ACKB38_001043"
     CDS             complement(15870..16673)
                     /locus_tag="ACKB38_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGL7979824.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(16701..17690)
                     /locus_tag="ACKB38_001044"
     CDS             complement(16701..17690)
                     /locus_tag="ACKB38_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7979825.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(17923..18942)
                     /gene="adhP"
                     /locus_tag="ACKB38_001045"
     CDS             complement(17923..18942)
                     /gene="adhP"
                     /locus_tag="ACKB38_001045"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGL7979826.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(19282..20094)
                     /locus_tag="ACKB38_001046"
     CDS             complement(19282..20094)
                     /locus_tag="ACKB38_001046"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGL7979827.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYSLAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            20284..21702
                     /locus_tag="ACKB38_001047"
     CDS             20284..21702
                     /locus_tag="ACKB38_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGL7979828.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            21755..22462
                     /locus_tag="ACKB38_001048"
     CDS             21755..22462
                     /locus_tag="ACKB38_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGL7979829.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            22557..23531
                     /gene="folP"
                     /locus_tag="ACKB38_001049"
     CDS             22557..23531
                     /gene="folP"
                     /locus_tag="ACKB38_001049"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGL7979830.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAYVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            23533..24855
                     /locus_tag="ACKB38_001050"
     CDS             23533..24855
                     /locus_tag="ACKB38_001050"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGL7979831.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEVIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPVTELTLTHFADSRATDENVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            24836..25390
                     /gene="folE"
                     /locus_tag="ACKB38_001051"
     CDS             24836..25390
                     /gene="folE"
                     /locus_tag="ACKB38_001051"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGL7979832.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            25433..26245
                     /gene="folK"
                     /locus_tag="ACKB38_001052"
     CDS             25433..26245
                     /gene="folK"
                     /locus_tag="ACKB38_001052"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGL7979833.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLVIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(26395..26712)
                     /locus_tag="ACKB38_001053"
     CDS             complement(26395..26712)
                     /locus_tag="ACKB38_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGL7979834.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            27068..27514
                     /gene="rplM"
                     /locus_tag="ACKB38_001054"
     CDS             27068..27514
                     /gene="rplM"
                     /locus_tag="ACKB38_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGL7979835.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            27533..27925
                     /gene="rpsI"
                     /locus_tag="ACKB38_001055"
     CDS             27533..27925
                     /gene="rpsI"
                     /locus_tag="ACKB38_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGL7979836.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(28188..28592)
                     /locus_tag="ACKB38_001056"
     CDS             complement(28188..28592)
                     /locus_tag="ACKB38_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGL7979837.1"
                     /translation="MLFFSISNDLLQLLTSWEKQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISEALT
                     HSDTVTTKTYVNTSNIVPLSAGQVAYQHLKNK"
     gene            complement(28968..30176)
                     /locus_tag="ACKB38_001057"
     CDS             complement(28968..30176)
                     /locus_tag="ACKB38_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGL7979838.1"
                     /translation="MNYIKRPHYLDFLRKHRDRPIIKVVSGVRRAGKSVLFQLYKEEL
                     LATGVDEDQIIFINFEDLSYYDLRHFQTLFAYIKDQLVSKKTYYIFLDEIQYVEKFEL
                     VADSLFILANVDLYLTGSNAYFMSSQLATNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYDEKIDYLRGIYNSILLNDIVTRLGKPNPTIIERIV
                     RTLLSSTGSLISTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDQKEDIRHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLEAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KSIIDWLLEK"
BASE COUNT         8780 a         5829 c         6803 g         8868 t
ORIGIN      
        1 tttagctgga tctacaaagg cggctttagc cactttatgg gcatcgtcaa tcttggaatc
       61 gtcgctattc caagcagcaa gagatttagc gtaaggatcg agtgcaagaa cagttttacc
      121 ttgacgctcg atttggtatt gataatagta gccagtgaaa tctgtgattc cgagtttgtt
      181 tgtgctgtct agagtttgtt tccaagttcc tctttcccct ttttcaagag cgacagttcc
      241 aactactttg tcagggtcat tcttgtcgta gacaacaaca gaaaccttat cagcacttgg
      301 tgaccaaagg gtcaaatcaa cttgttttcc ttcttctttt aggtcagctc ccagtttgcc
      361 atcatagctg tatgtctcat ctttcaggcg ccagcttgtt ttggtagtga attggtcgga
      421 attgtagcta acagtataag gatgttttgt gtcagagaaa tctccgctgt aggtcacttt
      481 cttaccagct tcatcgattg caacatcggt aatagttacc ttgtttccta ggtgattagt
      541 gatgttggag tgtttgagga tatcttcttt tttagcaccg acaagtgttg aaaagctact
      601 ttcaatgcta gaagtgccta cgtgttgggc tcctgtcata cggatatcat ggacatagta
      661 tggatttgtg taaatcgatt catcatcgtc ttttaggaaa atttggctat gatttttcaa
      721 atctgtgaac ttataatttt ctttacggat tttcacgtcg tctccttgtt tgctctcatc
      781 tagtaataaa aatccaaatt ctcttgcggc ttcattaaga ggaatgtcga tatagcggcc
      841 atatttgcct gtagccgtaa agtctgttcc gtcaggccat tgagcgctac ttggattttt
      901 cacatctccc cagtaccaga gagatttctt gtcatagttg ccatctgtgc ggtagtagtt
      961 gacgcgaaca gttcctgcag gctgtggctc gtaagagaaa accttgtaat cttggtctaa
     1021 ccaagcttcg ttcatttttg gaactagttt ttctacagat ttatcgccgg ttagattttt
     1081 tccagctgta ttgttgatga ggaagctaat tttcttggct tgttctccct ttaatttgac
     1141 atctaggtaa tagccgtagt catctttctt ggcatccttg aaggacaaag ctccgtttgg
     1201 ccagttttca gatggttttt caacatcgtc ccaagtccat agtccttgag catccttgtt
     1261 ttcttcagga agttttttga catggatacg gaagtagtta tcttcgatgg gctcttctgc
     1321 cttagtttca gttgttactg gactagtaga agtagttggc tcacttgaac gatcttgtcc
     1381 agtttgtggt gctggatcgg ctgggcttgc agcaggtttt tcttctgttt tctctaggga
     1441 agcgtttgca tttccaggtg aagaaatatc actttcgttc ttaccagagc cagctgctgt
     1501 atcagtaaga ggttgggcaa gggtggttgt atttctgttg ttgggttcgt tagtggttgg
     1561 tgtactttcg tcggatgaga tagttggtgt agccatggca agcaagacaa ggcttgcgcc
     1621 gataaggaca gaaccagttc catttttgag ggaacggatg ctgtagacca tttttttctc
     1681 agtgtgagat ggtgtttttc tcataatgat tctccaatca ataaatttct atatcagtat
     1741 agcatgtagt aaaacaatat gcaagcgttt tctttgattt gtgaaagaaa ttcttacttt
     1801 tgagaagttt tccgtactat tttatatatc agtataaatg acggagataa aaatgtcacg
     1861 taagccaagc cgtgttatta ggaagatgat gtgttctcag ctatttttat tcagaaattg
     1921 atctctaaca ttaccaataa aaaagaaaaa tatcttgaca agcaagggaa aattttgtta
     1981 caataataga cggtactttt tacttttggt ctctcaagag tgtacaggga cgtgctgaca
     2041 aatgttgcaa aagtacacac agatgatatc tgtcaccaag tgtatcatca ccaaaaataa
     2101 aaaaacacag gagaatgtag atgcctacaa ttaaccaatt ggttcgcaaa ccgcgtaaat
     2161 caaaagtaga aaaatctaaa tcaccagctt tgaacgttgg ttacaatagt cataaaaaag
     2221 ttcaaacaaa cgtttcttca ccacaaaaac gtggtgttgc aactcgtgtt ggaacaatga
     2281 cacctaaaaa acctaactca gcccttcgta aattcgctcg tgtacgtttg agcaacctta
     2341 tcgaagttac tgcctacatc ccaggtatcg gacacaactt gcaagagcac agcgtggtgc
     2401 ttcttcgcgg tggacgtgta aaagaccttc caggggtacg ttaccatatc gtccgtggtg
     2461 cacttgatac tgcaggtgtt aacgatcgta aacaaggccg ttctaaatac ggtactaaac
     2521 gtccaaaagc ataaggaaag gggataaaga gaaatgagtc gtaaaaatag agctccaaaa
     2581 cgtgacgtat tgccagatcc gctatacaat tcacaactag ttactcgtct tatcaaccgc
     2641 gttatgcttg atggtaaacg tggtactgct gcttcaatcg tttacggtgc ttttgagcaa
     2701 atcaaagaag ctactggcaa cgatgcactt gaagtatttg aaacagctat ggaaaacatc
     2761 atgcctgtac ttgaagtacg tgcacgtcgt gttggtggtt ctaactacca agtcccagtt
     2821 gaagttcgtc cagaacgtcg tacaacactt ggacttcgtt ggttggtaac aatcgctcgt
     2881 cttcgtggtg aacacacaat gcaagaccgt cttgcaaaag aaatcttgga tgctgctaac
     2941 aacactggtg cagcagttaa gaaacgtgaa gatactcacc gtatggctga agctaaccgt
     3001 gcattcgcac acttccgttg gtaagatagg atgcgaaagc gttaagaaag tcccagagaa
     3061 aatagggaat cgaagcaggt tgcggttgca accaatgaga ttcatctttt tctccagact
     3121 tttagcttga gctcaactaa atcatgatgc taggaacggt aaggatgcaa ggtaaaaata
     3181 ggaaactgac gcagtattcg acgaatacaa ggagttttat ctttttcacg cagcatcccg
     3241 ttccagctca catcggctaa ctaactttag cccgggttca aattagctaa atcgattagt
     3301 attagctata actcagctta ccatctcgta agttgaaacc aacaatagca tgaaaacatt
     3361 gagaacgggt aggtcctgcc tatccgtttt tattaaaatc gtgttataat agaatagaaa
     3421 tcaaaaataa ataggagaaa caaacctcat ggcacgcgaa ttttcacttg aaaaaactcg
     3481 taatatcggt atcatggctc acgtcgatgc cggtaaaaca acaactactg agcgtattct
     3541 ttactacact ggtaaaatcc acaaaatcgg tgaaactcac gaaggtgcgt cacaaatgga
     3601 ctggatggag caagagcaag aacgtggtat cacgatcaca tctgctgcga cgacagctca
     3661 atggaacaac caccgcgtaa acatcatcga cacaccagga cacgtggact tcacaatcga
     3721 agtacaacgt tctcttcgtg tattggatgg tgcggttacc gttcttgact cacaatcagg
     3781 tgttgagcct caaactgaaa cagtttggcg tcaagcaact gagtacggag ttccacgtat
     3841 cgtatttgcc aacaaaatgg acaaaatcgg tgctgacttc ctttactctg taagcacact
     3901 tcacgatcgt cttcaagcaa atgcacaccc aatccaattg ccaatcggtt ctgaagatga
     3961 cttccgtggt atcattgact tgatcaagat gaaagctgaa atctatacta acgaccttgg
     4021 tacggatatc cttgaagaag acatcccagc tgaatacctt gaccaagctc aagaataccg
     4081 tgaaaaattg attgaagcag ttgctgaaac tgacgaagaa ttgatgatga aatacctcga
     4141 aggtgaagaa atcactaacg aagaattgaa agctggtatc cgtaaagcga ctatcaacgt
     4201 tgaattcttc ccagtattgt gtggttcagc cttcaaaaac aaaggtgttc aattgatgct
     4261 tgatgcggtt atcgactacc ttccaagccc acttgacatc ccagcaatca aaggtattaa
     4321 cccagataca gacgctgaag aaattcgtcc agcatctgac gaagagccat ttgcagctct
     4381 tgccttcaag atcatgactg acccattcgt aggtcgtttg acattcttcc gtgtttactc
     4441 aggtgttctt caatcaggtt catacgtatt gaatacttct aaaggtaaac gtgaacgtat
     4501 cggacgtatc cttcaaatgc acgctaacag ccgtcaagaa atcgacactg tttactcagg
     4561 tgatatcgct gctgccgttg gtttgaaaga tactacaact ggtgactcat tgacagatga
     4621 aaaagctaaa atcatccttg agtcaatcaa cgttccagaa ccagttatcc aattgatggt
     4681 tgagccaaaa tctaaagctg accaagacaa gatgggtatc gcccttcaaa aattggctga
     4741 agaagatcca acattccgcg ttgaaacaaa cgttgaaact ggtgaaacag ttatctcagg
     4801 tatgggtgaa cttcaccttg acgtccttgt tgatcgtatg cgtcgtgagt tcaaagttga
     4861 agcgaacgta ggtgctcctc aagtatctta ccgtgaaaca ttccgcgctt ctactcaagc
     4921 acgtggattc ttcaaacgtc agtctggtgg taaaggtcaa ttcggtgatg tatggattga
     4981 atttactcca aacgaagaag gtaaaggatt cgaattcgaa aacgcaatcg tcggtggtgt
     5041 ggttcctcgt gaatttatcc cagcggttga aaaaggtttg gtagaatcta tggctaacgg
     5101 tgttcttgca ggttacccaa tggttgacgt taaagctaag ctttatgatg gttcatatca
     5161 cgatgtcgac tcatctgaaa ctgccttcaa gattgcggct tcactttccc ttaaagaagc
     5221 tgctaaatca gcacaaccag ctatccttga accaatgatg cttgtaacaa tcactgttcc
     5281 agaagaaaac cttggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgtagatgg
     5341 tatggaagca cacggtaaca gccaaatcgt tcgtgcttac gttccacttg ctgaaatgtt
     5401 cggttacgca acagttcttc gttctgcatc tcaaggacgt ggtacattca tgatggtatt
     5461 tgaccactac gaagatgtac ctaagtcagt acaagaagaa attattaaga aaaataaagg
     5521 tgaagactaa tccgtcctca ctctagaagg aagtcactta gtggcttcct tttgtcttta
     5581 gaaaatacct ctaaatatgg taaaatagta gaagaataat gtgaggaaaa tgaatgtcaa
     5641 atagttttga aattttgatg aatcaattgg ggatgcctgc tgaaatgaga caggctcctg
     5701 ctttagcaca ggccaatatt gagcgagttg tggttcataa aattagtaag gtatgggagt
     5761 ttcatttcgt attttctaat attttaccga ttgaaatctt tttagaatta aagaaaggtt
     5821 tgagcgaaga attttctaag acaggcaata aagctgtttt tgaaattaag gctcggtctc
     5881 aagaattttc aaatcagctc ttgcagtcct actataggga ggctttctct gaaggtccat
     5941 gtgctagtca aggttttaag tccctttatc aaaatttgca agttcgtgct gagggtaatc
     6001 agctatttat tgaaggatct gaagcgattg ataaggaaca ttttaagaag aatcatcttc
     6061 ctaatttagc caaacaactt gaaaagtttg gttttccaac ttttaactgt caagtcgaga
     6121 agaatgatgt cctgacccaa gagcaggaag aggcctttca tgctgaaaat gagcagattg
     6181 ttcaagctgc caatgaggaa gcgctccgtg ctatggaaca actggagcag atggcacctc
     6241 ctccagcgga agagaaacca gcctttgatt ttcaagcgaa aaaagctgca gctaaaccca
     6301 agctggataa ggcggagatt actcctatga tcgaagtgac gacagaggaa aatcgtctgg
     6361 tatttgaagg ggttgttttt gatgtggagc aaaaagtgac tagaacaggt cgtgttttaa
     6421 tcaactttaa aatgacggac tatacttcaa gtttttctat gcaaaagtgg gttaaaaacg
     6481 aggaagaggc ccagaagttt gacctcatca agaagaattc ttggctccga gttcgaggga
     6541 atgtggagat gaataacttc acacgcgatt tgactatgaa cgtacaggat ctgcaggaag
     6601 ttgttcacta tgagcggaag gatttgatgc cagaaggtga gcgtcgggtt gagtttcatg
     6661 ctcatactaa catgtcgact atggatgctt tgccagaggt cgaagagatt gttgcaacag
     6721 ctgctaagtg gggacacaag gcggttgcta tcacggacca tgggaatgtc cagtcctttc
     6781 cacatggcta taaggcggct aagaaagcgg gaatccagct gatctatggg atggaagcca
     6841 atatcgtgga ggaccgtgtc cctatcgtct ataacgaagt ggagatggac ttgtcagaag
     6901 caacctacgt ggtctttgac gtggaaacga cgggactttc agctatctat aatgacttga
     6961 ttcaggttgc ggcttctaag atgtacaagg ggaatgttat tgctgaattt gatgaattta
     7021 tcaatcctgg gcatcccttg tcagccttta ctacagagtt aactggaatt acagatgatc
     7081 atgtcaaaaa tgccaaacca ctagaacaag ttttgcaaga attccaagaa ttttgcaagg
     7141 atacggtcct agttgcccac aatgctacct ttgacgttgg ctttatgaat gctaattatg
     7201 agcggcatga tcttccaaag attagtcagc cagttattga tacgctggag tttgctagaa
     7261 acctctatcc tgagtataaa cgccatggtt tggggccttt gaccaagcgt tttggtgtgg
     7321 ccttggaaca tcaccacatg gccaactacg atgcggaagc gactggtcgt ctgcttttca
     7381 tctttatcaa agaggtagca gaaaaacatg gtgtgaccga tttagctaga ctcaacattg
     7441 atctaatcag tccagattct tacaaaaaag ctcggatcaa gcatgcgacc atctatgtca
     7501 agaatcaggt aggtctaaaa aatatcttta agctggtttc cttgtctaat accaagtatt
     7561 ttgaaggagt gccacggatt ccgagaacgg ttctagatgc ccatcgagag ggcttgattt
     7621 taggttcagc ctgttcagag ggtgaagttt ttgacgtggt cgtttctcaa ggtgtggatg
     7681 cggcggttga ggtggccaag tattatgatt ttatcgaggt catgccaccg gctatctatg
     7741 cacccttgat tgccaaagag caggtcaagg atatggagga actccagacc attatcaaga
     7801 gtttgataga ggttggagac cgccttggca agcctgttct ggctacggga aatgttcact
     7861 atatcgaacc ggaagaagag atttatcgtg aaattatcgt ccgtagtttg ggacagggtg
     7921 cgatgattaa tcgaactatc ggtcatggtg aacatgccca accagcacca cttccaaagg
     7981 ctcattttcg aacgactaat gagatgttgg atgaatttgc ctttttggga gaggaactgg
     8041 ctcgtaaact ggttattgaa aacaccaatg ccttggcaga aatatttgaa tccgttgaag
     8101 tcgttaaggg tgacttgtat acgcctttca tcgacaaggc tgaagaaaca gttgctgagt
     8161 tgacctataa gaaagctttt gagatttatg gaaatccgct gccagatatt gttgatttgc
     8221 ggattgaaaa agaattaaca tccatactgg ggaatggatt tgctgtgatt tatctggcat
     8281 cgcagatgct ggtgcaacgt tctaatgaac ggggttattt ggttggttct cgtgggtctg
     8341 tcggatctag tttcgttgcg accatgattg ggattacgga ggtcaatcct ctctctcctc
     8401 actatgtctg tggtcagtgt cagtacagtg agtttatcac agatggttcg tacggttcag
     8461 gatttgatat gccccataag gactgtccaa actgtggtca caaactcagt aaaaacggac
     8521 aggatattcc gtttgagacc ttccttggtt ttgatgggga taaggttcct gatattgact
     8581 tgaacttctc gggagaagat cagcctagcg cccacttgga tgtgcgtgat atctttggtg
     8641 aagaatatgc cttccgtgcg ggaacggttg gtacggtagc tgccaagact gcctatggat
     8701 ttgtcaaagg ttacgagcga gattatggca agttttatcg tgatgcagaa gtagaacgcc
     8761 tcgctcaagg agcggcgggt gtcaagcgga caacaggcca acacccgggg ggaatcgttg
     8821 ttattccgaa ctacatggat gtctacgatt ttacgcctgt ccagtatcca gcagatgatg
     8881 tcacggctga atggcagacc actcacttta acttccacga tatcgatgag aacgtcctca
     8941 aactcgatgt actgggacat gatgatccga ctatgattcg aaaacttcag gatttgtctg
     9001 gtattgaccc taataaaatt cctatggatg acgaaggcgt gatggcactc ttttctggga
     9061 ctgatgtgct aggggtaaca cctgaacaaa ttggaacgcc tacgggtatg ttggggattc
     9121 cagagtttgg aacaaatttc gtacgtggaa tggtagacga aacccatccg acaacctttg
     9181 cggaattgct tcagctgtct ggtctgtccc acggtactga tgtttggttg gggaatgctc
     9241 aggatctgat taagcaagga atagcggacc tatcgactgt tatcggttgt cgggacgaca
     9301 tcatggttta cctcatgcat gcgggtctgg aacctaagat ggcctttacc attatggaac
     9361 gggtacgtaa gggtttgtgg ctaaagattt cagaagagga gagaaatggc tatatcgaag
     9421 caatgaaggc taataaggtg ccagagtggt atatcgaatc ctgtgggaaa attaagtaca
     9481 tgttccctaa ggcccatgcg gcagcctacg ttatgatggc cttgcgtgta gcttacttca
     9541 aggttcacca tcctatttat tactactgtg cttacttctc cattcgtgct aaggcttttg
     9601 atatcaagac catgggtgcg ggcttggagg tcatcaagcg cagaatggaa gaaatctctg
     9661 aaaaacggaa gaacaatgaa gcctctaatg tggaaatcga tctctataca actcttgaga
     9721 ttgtcaatga gatgtgggaa cgaggtttca agtttggtaa attagatctc tactgtagtc
     9781 aggcgacaga gttcctcatc gacggggata cccttatccc accatttgta gcaatggatg
     9841 gtctgggaga gaacgttgcc aagcaactgg tgcgggcgcg tgaagaggga gaattcctct
     9901 ctaaaacaga actacgcaag cgtggtggac tctcatcaac cttggttgaa aagatggatg
     9961 agatgggtat tcttggaaat atgccagagg ataaccagtt gagtttgttt gatgagttgt
    10021 tttaaaaaat tgcttaataa tctattaaaa gaggctaacg tatatccaat agatttacat
    10081 tagctttctt ttttgttaaa atagtctatg gaaagagggt gagagtatgt caaagatgag
    10141 tataagcatc cgtctggata gtgaggttaa ggagcaggcc caacaggtgt ttagtaatct
    10201 gggaatggat atgacaacag ctattaatat tttccttcgt caggcaattc aatatcaggg
    10261 attacctttt gatgttagac tagacgaaaa tcggaagttg ctccaagcgt taacggattt
    10321 agaccaaaat cgtaatatga gccagtcttt tgaatcagtc tcagatttga tggaggactt
    10381 acgtgcttaa gattcgttat cataaacagt ttaaaaaaga ttttaagttg gctatgaagc
    10441 gtggtttgaa ggcagaatta ttagaagaag ttttgaattt tctggttcaa gaaaaagaac
    10501 atcctgccag aaatcgtgat cattcattga cggcatccaa gcattttcaa ggagttcgtg
    10561 aatgccatac ccagccagat tggcttttgg tttataaagt agacaagtcg gaattgattt
    10621 taaatttgct gaggacaggc agtcacagtg atttatttta atctatttta agggggttct
    10681 catgaaacta agaatatttg cggaagataa gccggctaag aaggtatttg aatatcaatt
    10741 agaacttgct gatcgtacaa ttcttctatc gacagcactc ttgtcaggtg ctattgcttt
    10801 agcaggaatc ttttctgctt tgaaagaaaa ataaaaatag aaaagagaaa acagaatggt
    10861 tttaccaaat tttaaagaaa atctagaaaa atatgcgaaa ttgttggttg cgaacggaat
    10921 taacgtgcaa cctggtcaca ctttggctct ctctattgat gtggagcaac gtgaattggc
    10981 acatctaatc gtgaaagaag cttatgcctt gggtgcgcat gaggtcatcg ttcagtggac
    11041 agatgatgtg attaaccgtg agaaattcct ccatgccccg atggagcgtt tggacaatgt
    11101 gccagaatac aagattgctg agatgaacta tctcttggag aataaggcta gccgtcttgg
    11161 agttcgttca tctgatccag gtgccttgaa cggagtggac gctgacaagc tttcagcttc
    11221 tgctaaagct atgggacttg ccatgaagcc tatgcgtatc gcaactcaat ctaacaaggt
    11281 tagctggact gtagcagctg cagcaggact tgagtgggct aagaaagtct tcccaaatgc
    11341 tgcgagcgac gaagaagcag ttgatttcct ttgggaccaa attttcaaaa cttgccgtgt
    11401 ctacgaagca gatcctgtta aggcttggga ggaacatgca gccattctca agagcaaggc
    11461 cgatatgctt aataaggagc aattttcagc ccttcactac acagcgccag gaacagattt
    11521 aacacttggt ttgccaaaga accacgtttg ggaatcagct ggtgctgtca atgcacaggg
    11581 cgaagaattc ttgccaaata tgccaacaga agaggtcttc acagcgcctg acttccgtcg
    11641 tgcagatggt tatgtcactt ctacaaaacc gcttagctac aacggaaata tcattgaagg
    11701 cattaaggtg acctttaagg atggacaaat cgtagatatc actgctgaga agggtgatca
    11761 ggttatgaaa gaccttgtct ttgaaaatgc gggtgcgcgt gccttgggtg aatgtgcctt
    11821 ggtaccagat ccaagtccaa tttctcagtc aggcattacc ttctttaaca cccttttcga
    11881 tgaaaatgcg tcaaaccact tggctatcgg tgcagcctat gcgactagcg ttgttgatgg
    11941 agcggagatg agcgaagagg agcttgaagc tgcagggctt aaccgttcag atgttcacgt
    12001 agactttatg attggttcta accaaatgga tatcgatggt attcgtgagg atggaacgcg
    12061 ggtacctctt ttccgtaatg ggaattgggc aaattaagga gataatatgt taggaagtat
    12121 gttcgttggt ctcctagtgg gatttttagc aggtgctatg accaatcgtg gagagcgaat
    12181 gggatgtttt ggaaaaatgt ttctcggttg gatcggagcc tttctaggtc acttgctctt
    12241 tggaacttgg gggccagttt tatcaggaac agctattatc ccagcgattt taggagccat
    12301 gattgtttta gctatttttt ggagacgagg aagttaattt cctaaatctg atactcaatg
    12361 aaaatcaaag agcaaactag gaaactagcc gcaggatgct caaagcactg ctttgaggtt
    12421 gcagatagaa ctgacgaagt cagctcaaaa cactgttttg aggttgcaga tagaactgac
    12481 gaagtcagta acatatatac ggcaaggcga cgttgacgcg gtttgaagag atttttgaag
    12541 agtatgaaac gaaaaggagg ttggtcattg tactagcctc cttttgatat gatataatag
    12601 ttctatgaga ttagataaat ttttagttgc ctgtgcggtg ggaagtcgga ctgaggtcaa
    12661 aaacttgctc aaggctgggc gcgtgactgt aaatggtaaa aaagaaaaat cagctaaatt
    12721 gcagattgat gaaaaaatag atgagattcg ctttgatggg caagtgttgg agtatgaaga
    12781 gtttgtctac tacatgatga acaagcccaa aggagttatc tcagcgactg aggatcccaa
    12841 gcacagaacc gttctggact tgttggatga cttggcgcgg agtaaggaag ttttcccagt
    12901 aggacgcttg gatattgaca cgcatggtct tttgctcttg accaatgatg gtcagctggc
    12961 tcatgttctt ctttcgccca agcgtcatgt ggacaagact tatctggcac aagtcaaggg
    13021 aatcatgacc caagaagatg tggagacatt tgctgagggt attcctctca aagactttac
    13081 ctgtcaaccc gctatactgg agcttgtatc catagataca gaaaagaatc aaagccaaat
    13141 ccgtgtgacc attgcagaag ggaagtttca tcagatcaag cgtatggtgg gctactgtgg
    13201 caaggaagtg gtggacttgc aacgattgac tatgggaact ttagtattag atgagaacct
    13261 agaacgaggg gaatggcgtc gcttgaccaa ggaagaatta gaaattctcc gcgctaatat
    13321 tatttaattt agtttaatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
    13381 gctgtacttg agtacggcaa ggcgacgttg acgtggtttg aatttgattt tcgaagagta
    13441 taaattcgaa aaaggtgagg gacaatgtcc tcgcctttta tgttttttag ttgcttcctt
    13501 tgtgaaaaga gttataatag actgtagaat aaaaggagga atctatgaac gcgattcaag
    13561 aatcatttac tgataaacta tttgccaact atgaagcaaa tgtaaaatac caagcgattg
    13621 aaaatgctgc cagccacaac ggaatttttg cagctctgga acgtcgccaa agccatgtag
    13681 acaacacacc tgttttctca ttggatttaa ccaaggacaa ggtcactaac cagaaagcgt
    13741 ctggtcgttg ctggatgttt gcggctctca acaccttccg ccacaaactc atctcgcaat
    13801 acaaattgga gaactttgag ttgtcacaag cccacacttt cttctgggat aagtatgaga
    13861 aatcaaactg gttcttggag caagtcattg cgacttcaga ccaagaattg actagccgca
    13921 aggttagctt cttactccaa acacctcaac aagatggcgg tcaatgggat atggtcgttt
    13981 ccctctttga aaaatacggt gtcgtgccta agtcagttta tcctgagtct gtttcatcta
    14041 gcagcagtcg tgagctaaat gcgatcctta ataaattgct tcgtcaagat gctcaaatct
    14101 tgcgtgactt gcttgtttct ggtgcagatc aagcgactgt tcaagctaag aaagaagacc
    14161 tcttgcaaga aatctttaac tttcttgcta tgtcattagg acttccacca cgcaagtttg
    14221 actttgctta tcgcgataaa gataacaact acaaaagtga aaaaggaatc acaccacaag
    14281 agttttacaa gaaatatgtc aatcttcctt tagaagacta cgtttctgtt atcaatgctc
    14341 caactgctga taaaccttac ggaaaatctt acacagttga gatgttgggg aatgtggttg
    14401 gtagccgtgc agttcgctac atcaacgttc caatggagcg cttgaaagaa ttggcgattg
    14461 cccaaatgca agcaggtgag actgtttggt ttggttctga tgtcggccag ctcagcaacc
    14521 gtaaggctgg catccttgcg acagatgttt atgactttga atcaagcatg gacattaaac
    14581 ttactcaaga caaggctgga cgtttggact atagtgaaag cttgatgacc cacgccatgg
    14641 tcttgacagg tgttgacttg gacgaaaatg gtaaatcaac caagtggaag gttgaaaact
    14701 catggggaga caaggtcggt acagatggtt actttgttgc ctcagacgct tggatggacg
    14761 aatacacata ccaaatcgtt gttcgtaagg aattgctgac agcagaagaa caagctgcct
    14821 atggagcaga accaatcgta cttgcaccat gggatccaat gggagccttg gctgaataaa
    14881 agcatagaaa aaaggaatca gattttagaa cctggttcct ttttagttgc ttgattacat
    14941 gatgtgaaga acatgtgcca caatacccac tgcgaagagg gcaaggataa tagtgattgg
    15001 agatactttt ttcttaagta agtacatgca aagtaaagta aggagtagtc ctgataatcc
    15061 aggaatcaac atatccaagt tttgttggaa agtagtaact ttttcaggag tttgagacaa
    15121 tccttgtcct acttgtgcga atgcttcttg gataccttta gacccttctg gcaatttatc
    15181 ccaatggata taagcctttt catctagttg aactttagaa acatcgaaag caaatttaat
    15241 atttacccag cgttgaacaa ggacagcaag aatgaacatc ccaaggatag aagctccttt
    15301 agtgatatct tgaaggatac caccagacat atctttagtg atttctgatc cagccttgta
    15361 tccaatctct tgaacatacc acaagaatga catacgaatc aagttccatg caacaaagaa
    15421 gaggagtggc cccaagatat tgccagtaag ggcaagtgaa gcaccgagag atccaaggat
    15481 tgggcgtact gtaaaccaga atactgggtc accgatacca gcaagaggtc ccatcatacc
    15541 gattttaacc ccttggatag cagcgtcatc gatttccaca ccgttagcac gttcttcttc
    15601 aagcgcaaga gtaaccccca tgactggagc agctacgtat ggatgagtgt tgaagaactc
    15661 aaggtgacgc tcaagagcag cgatttgatc ttctttttta gtatagagtt ttttgatagc
    15721 tggaatgagt gtataagccc agcccaagtt ttgcatccgt tcaaagttcc aagacccttg
    15781 taagaaggtt gaacgccacc aaactttttt acgatctgat ttagttaatt gaagtttttc
    15841 agtcatgatg ttttcagtcc tttcttatct tagtagtctt ctaggatatc gccgattggg
    15901 tcgttagaag ttgcggctcc tccgccacca tttccaccag ttttagaaag gtgaaggtag
    15961 ataagagcga tagcaacgcc gatagcaccg aatccgatta gagtaatatc tgacacagca
    16021 gcgagaacga aaccaagagc gaagaatggc catacttcac gagttgccat catgttgata
    16081 accatggcgt aaccaacggc aacgaccata ccaccaccga tagccatacc atctttgagc
    16141 cagtctggca tggcactaag gatactttgt acagtttcag ttggtaccat aaggagaaga
    16201 gctgcaggaa gcgcgatacg aagtccttgg aaaagtagcg cgatgaaatg cgcacgctcc
    16261 acagcgccga agtcaccttt tttagcggca gcatctgcag tatgaaccaa accaactgaa
    16321 attgtacgaa caatcattgt caagaaaagt ccagctacag caagagggat agcaaccgct
    16381 tgggcaacac cgataccagt cttggtaaag tcaccaccaa gaaccatgat aatggcagca
    16441 gcgacagaag caagtgcagc atcaggagcg atagcagcac cgatatttga ccaaccaagg
    16501 gcaatcattt gaagcgatcc accgaggata atccctgctt ccaagtgacc tgttacaagc
    16561 ccaataaggg tacaggctac aagtggttgg tgaaattgga actggtcgag gatgccttca
    16621 agacctgcaa agaaggctac aacgactact aaaaccatag aaataataga catgtttaaa
    16681 atcctttcat aaataatggc ttatttgaca ttggctttgt taatcaagtc aaacaaatct
    16741 tttttagaat catttggtac tttacggaca tcaaattcaa cacccaagtc acgcattttt
    16801 tcaaatgtag caacgtcttc tttgtccata gacaaaacgg tattgaccaa tgttttacct
    16861 gttgagtgag ccatagaacc aacattaaga gtcttgattg gcacgccgcc ttcgatggca
    16921 cgaagggcat cttgaggtgt ttcaaacaag ataagggcat gtgtttctcc aaaacgtggg
    16981 tcttttgaaa tctcaatcag tttttgaatt ggaaccacgt tagccttgac attacctgga
    17041 gctgcttgtt taatcaattc tttacgaagg tcgtctttag ccacgttatc tgaagcaacg
    17101 atgatacgat ttgcttttga atctggagtc caagcagttg caacctgacc gtgaagtaga
    17161 cgtgtgtcaa gacgggcaag attgattttc aatttaccgt ctccgataac agttccttct
    17221 gggatagcag tttgggcaac tggagcagct gcagcgcttg caacttcttc gactggattt
    17281 agctcttctg gaagagcttt gatgccatct ttggcttctt taatgatatt agcagcgact
    17341 ttttctacac ctgcagcagc gtccatgagg cgctctgtgt aggcttgaat caacatcggt
    17401 aagttaagtc ctgtgatgat ggcaaactta cgctcaggat tttctcccat cacgcgacta
    17461 gcttggttaa atggagaacc actccaaagg tcagccaaaa ctagaacctc atcttctgcg
    17521 tcaaatgcag caacagcgtt attaaactta gcgtatagat catcaggacc ttcatttggc
    17581 ataaaggtca caacttgaac cttttcttgt tcaccaaaga tcatagatcc tgactgatga
    17641 atacccgcag caaattcgcc gtggctcgca ataatgattc cgatactcat tattgtcatt
    17701 cctccttttt tgttttagtt ttcatttaag aaaactttaa gactgtttaa gtataaaccg
    17761 ttttcataaa aaaagcaact atttatcata aaaaattaaa aaatttttat ttttgtatgt
    17821 tgatatgttg agtttttgag gagtaatctt gaattcttat ttaaaattta atataaaaaa
    17881 gttggaagaa gcttccaact tttaaaatag gtttctatta gattagtggg tgaagtcgag
    17941 taccatacgt ccttggattt ggcctttttc catttcgtcg aaaatggcaa cagcatcttc
    18001 tactggacgt ttttgaacaa ctgggactac cagaccttct gcaccaaatt ggaaggcttc
    18061 ttctaagtct ttacgagttc cgacaagaga accgatgact tggattccat cgaggactgt
    18121 tttaacgatg ctgagttcca tcatttcaga aggaagacca acagcgacga cgcgaccacc
    18181 agcacgaatg gagtcaacag cctggttgaa ggcaacttta gacacagcag ttacgacagc
    18241 tgaatgagct cctccatcag ttttttcttt aatgagtcca gctacatctt caacttcgag
    18301 gccgttaatc acaatgtcag cgcctacttc ttttgcaagg gcaagtttgt cattgttgat
    18361 atcgactgcg ataacatgag cattgaatac ttttttagcg tattgaacag cgaggttacc
    18421 aagtccacca gcaccgtaaa gaacaaccca ttggcctggt tcaacttttg cttctttgat
    18481 agctttatag gttgttactc cagcacatgt gatagaagaa gcttgggctg gatcaagtcc
    18541 gtcaggaact ttgacagcat agtcagcagt tacgatacat tgttcagcca taccaccgtc
    18601 tactgagtag ccagcatttt tcactgtacg gcaaagggtt tcgcgaccag ttgtacagta
    18661 ttcgcaagtg ccacatcctt caaagaacca agcaacgctg acgcggtcac cgactttaag
    18721 gcttttcaca tctggagcaa tctctttaac gataccgata ccttcgtgcc caagaacacg
    18781 tcctgggact tgaccaaagt caccatgagc aacgtggagg tcggtgtggc aaacgccaca
    18841 gtattcaact tctacaagtg cttccccagt ttcaagtgga cggagtactt tttcttcaat
    18901 agcaacacca gtgctttctg gatttacaac aacagctttc atagctatcc tccttgatat
    18961 tgaccgagaa cagggacaac gggactggat tgattttttg tcaacagagt cgagtgtgac
    19021 gagctctctt gtatttatat gtgaagtata tcacaaaaga aagccttttc caaggttttg
    19081 gagacaaaaa gtagaacaat cgattaactg gttgatgaga ctgtgaaaag agcacaaaga
    19141 ccagcttgca agctggtcat actcttcgaa aatctcttca aaccacgtca gcttcatcta
    19201 caacctcaaa gcagtgcttt gagcaaccta cggctagctt cctaagtttg ctctttgatt
    19261 ctcattgagt atcagtatag gttataagaa taagtcttgt agggttttgg caaccccatc
    19321 ttggtcgttg gtaagggaaa tttgctcatc tgcataaggg agtagctctg gattggcatt
    19381 tttcatggca taacccttcc cagcaaaagc gagcatttcg gtatcattgt gttcatctcc
    19441 aaaggcaatc aaatcttttt tgtcacgatt cattatcttg agcaagtagt ccaaagcaaa
    19501 ggccttgttg acaccttttg gggtacattc aaggatattg agcggacctc cccaggtatt
    19561 gatagaaagt tgatgctggt agaaggcgtt catttctttt gccaaggaat atttgtcact
    19621 ggctctagtc tgcaagagga tacagttagg gtccttggtc accaattcag gctggaattg
    19681 atcttcaggc tggaaagctt ctacaccaaa tagtttggga ttggcaattt cttcattggg
    19741 atttgtaatg tagaattttt tacgatattc tccagcgata aaatcggctt gaatgtcctc
    19801 tgaacgttga accatatcta gcagatattt tttgtctaca gtcaaacact tttcaaaatc
    19861 ccaaacttgg tctggtaaat gagtaaggga tccgttgaag ttaatcatag gagtgtctaa
    19921 gcccagttca cggtaaaaat cttttgacat acggtaaggg cgacctgtcg taataataac
    19981 ctgatggcct ttttcagcaa cttttttgat ggttcttttg gtaaagtcag aaatttgact
    20041 gtctgagttg agcagggttc catccaggtc aactgcaata atttttttcg tcatatggac
    20101 ctttctagtg tgttttcaga taagatgtct tctattataa cagaaagcag gcagaaaagc
    20161 agattccaaa caaaaaatga aattggatta atttcttaaa caaatcaaac aaagacattg
    20221 aaaaagtgaa tgataaatga tataattcta ttattgttcg taaaaattaa aaggagattg
    20281 atgatggaca aattatttaa actaaaagag aacggtacag acgttcgtac agaggttctc
    20341 gctggtttaa caactttctt tgcaatgagc tatattctct ttgtaaaccc acaaatactt
    20401 tcacaaacag gaatgcctgc tcagggcgtc ttcctagcga cgattattgg tgcagtagcg
    20461 ggtaccttga tgatggcttt ttatgctaac ttaccttatg cccaagcgcc aggtatggga
    20521 ctcaatgcct tctttacctt tacagttgta ttcgggcttg gttattcttg gcaagaagcc
    20581 ctagctatgg tcttcatctg tgggattatt tcattgatta ttaccttgac aaatgttcgt
    20641 aaaatgatca ttgaatcgat tcccaatgct cttcgctcag ctatttcagc tggtatcggt
    20701 gtcttccttg cctatgtagg gattaagaat gctggacttt tgaaattcac gattgatcca
    20761 ggcaactata ctgttgtagg agaaggggct gacaaagctc aagcaacgat tgcagcaaac
    20821 tcttcagcag ttccaggatt ggtcagcttt aataatccag ctgttttagt ggctcttgca
    20881 ggacttgcca ttactatctt ctttgtcatc aaagggatta aagggggaat tattctctct
    20941 atcttgacaa caactgttct tgctattgca gttggtttgg ttgatttgtc tagtatcgat
    21001 tttgctaata accatgttgg tgcagctttt gaagatttga agacaatctt tggtgcagct
    21061 cttggttcag aaggattggg agctttggtt tcagatacag ctcgcttgcc tgaaactctg
    21121 atggccattc ttgccttctc attgacagat atttttgaca caattggtac cttgatcggt
    21181 acaggtgaaa aagttggtat cgtagcgaca aatggtgaaa atcaccaatc agccaaattg
    21241 gataaggctc tttactctga tttgattgga acgacagtcg gtgccattgc aggtacttca
    21301 aacgtaacga cttatgttga gtctgctgct ggtatcggtg caggtggacg tactggtttg
    21361 acagccttgg ttgtagctat ctgttttgcg atttcaagct tctttagccc acttctagcg
    21421 atcgtaccaa cagcggctac agctccaatc ttgattatcg ttgggattat gatgcttggt
    21481 agcttgaaaa atatccattg ggatgatatg tctgaagcag ttcctgcctt cttcacatct
    21541 atctttatgg gattcagcta ctctatcact caagggattg cagttggttt cttgacttac
    21601 actttgacta agcttgttaa aggtcaagtt aaagatgttc atgtcatgat ttggattttg
    21661 gatgccttgt ttatccttaa ctacatcagc atggccttat aatagaatga cccaggggga
    21721 tttcccccct tttttaatac aaaggagata ggtgatgaaa gagaaaaata tgtggaaaga
    21781 attgttgaat cgtgcaggct ggattttggt ctttttactt gccgtccttt tatatcaggt
    21841 tcccctagtg gttacctcta ttttgacttt aaaagaagta gccctgctac agtcagggct
    21901 gatagttgct ggcctttcaa ttgtggttct ggctctattt attatgggag ctcgtaaaac
    21961 caagttagct agttttaatt tttctttttt tagagctaaa gatttggcac gtttgggctt
    22021 gagttatcta gttattgtcg ggtcaaatat acttggttcc attttattgc aactgtcaaa
    22081 tgagacgaca acagctaacc agtctcagat taatgatatg gttcaaaata gttcgttgat
    22141 ttccagtttc ttcttgctag ccttgcttgc tccgatttgt gaggaaatct tgtgtcgtgg
    22201 gattgttcct aaaaagattt tccgaggcaa ggagaacttg ggatttgtag tcggtacgat
    22261 tgtgtttgct ttattgcatc aaccaagtaa tttaccttct ttattgattt atggaggtat
    22321 gtcgacagtt ctatcttgga cagcctacaa gacccaacgt ttggaaatgt cgatcttgct
    22381 tcacatgatt gttaatggga ttgctttctg tttgttggct cttgtggtga ttatgagtcg
    22441 gacattagga atttctgttt aaaagttttt atgtaggaac cgacctcttt ctaccaggga
    22501 aagatgaatg caatcgtgtc catctttttc tttttatggt aaaatagaaa aataatatga
    22561 tgaaaatcct tgagggagtg accgatatgt caagtaaagc caatcatgca aagacagtta
    22621 tttgcggaat tatcaatgta accccagact ccttttcgga cggtggtcaa ttttttgctc
    22681 ttgagcaggc gctccagcag gctcgtaaat tgatagcaga aggagccagt atgctagata
    22741 tcggcggaga atcgactcgg ccgggaagta gctatgttga gatagaagag gaaatccagc
    22801 gtgttgttcc agtgatcaaa gcgattcgca aggaaagtga tgtcctcatc tctattgata
    22861 cttggaagag tcaagtagca gaggctgctt tggctgctgg tgccgatcta gtcaatgata
    22921 tcactggtct tatgggtgat gagaaaatgg cttatgtggt agctgaagcg agagcgaaag
    22981 tggtcatcat gtttaaccca gttatggctc gacctcagca tcctagttcg cttatcttcc
    23041 ctcattttgg ttttggtcaa acctttacag aaaaagagtt agctgacttt gaaacattgc
    23101 caatcgaaga cttgatggtg gctttctttg aacgagcact agcgagagcg gcagaagctg
    23161 gtattgcacc agaaaatatc ctgttggatc caggaattgg ctttggtctg accaagaaag
    23221 aaaatctgct tcttttacgg gacctggata aactacatca gaagggctat ccaatctttc
    23281 tcggagtgtc gcgcaagcga tttgtcatca atatcctaga ggagaatggt tttgaagtca
    23341 atcctgagac agagcttggt ttccgaaatc gggacacggc ttcggctcat gtaactagta
    23401 tcgctgcgag acagggtgta gaagtggtgc gcgtgcatga cgtagctagt cacaggatgg
    23461 cagttgaaat tgcctctgcc attcgtctgg ctgatgaagc ggaaaattta gatttaaaac
    23521 aatataaata agatgaaaga aattgaaaac aatcagtgga ttgctaacta ccggacggat
    23581 caaccgcatt ttggcttgga acgaatggtg gaactgttag ctttgcgtgg caatccccat
    23641 ctcaaactca aggtcctcca tatcggaggg actaacggca agggctcgac tattgctttt
    23701 ttgaaaaaga tgctagaaaa gctagggttg agagttggcg tgtttagctc gccctatctc
    23761 attcattaca cagaccagat tagcatcaat ggggaatcga tctcagaagc gaggctagaa
    23821 gctctcatgg cagactatca gtctttgctg gagggagaag cggtcgccaa tttacagggc
    23881 acaaccgagt ttgagattat cacagccctg gcctatgact actttgcctc agagcaagta
    23941 gatgtggcca tcatggaagt tggcatgggt ggacttttgg atagtaccaa tgtctgtcag
    24001 cccattttga caggaattac aactattggc ttggatcatg tggctctact tggtgacacc
    24061 ttggaggtca tagcagagca gaaggcaggt attatcaaac aagggatgcc cttggtaaca
    24121 gggcgtattg ctccagaagc cttggctgtg attgaccgca ttgcggaagg gaaagatgcg
    24181 ccgagacttg cctacgggac agattatcag gttcgtcatc aagaaagtgt ggtgacaggg
    24241 gaagtctttg actatacaag tgctgtcaga caaggtcgct tccagactag cctgcttggt
    24301 ttgtaccaaa tagagaatgc tgggatggcc atagctttac ttgatacttt ttgtcaagaa
    24361 gatggtcgag agctagcaag caatgatttt cttggtcaag ccttggaaga aacaagttgg
    24421 ccagggcgtt tggaaatcgt gtcaagagat cccttgatga ttttggatgg agcccacaat
    24481 ccccatgcta tcaaggcctt gttggtaacc ttgcaagaac gttttgcgga ttatcataag
    24541 gaaatcctct tcacttgtat caaaaccaag gccttggagg atatgttgga cttgctggga
    24601 gccatgccag ttaccgagct tactctaaca cattttgcgg atagtcgggc gacggatgaa
    24661 aacgtgctga aagaggcagc taagtctaga aatctcagct accaagattg gcatgatttt
    24721 ctagagcaga atttgacaga taaaaaagaa gagaaacaaa cagttaggat tgtcacaggt
    24781 tccttgtatt tcttgagcca agtgagggcc tatctgatgg agaggaagaa cgagaatgga
    24841 tacacaaaag attgaagcgg ctgtaaaaat gattatcgag gctgtaggag aggacgctaa
    24901 tcgcgagggc ttgcaggaaa cacctgctcg tgtagcccgt atgtatcaag agattttttc
    24961 aggtcttggt caaacagcag aggaacattt gtcaaaatcc tttgaaatta ttgacgataa
    25021 tatggtggta gaaaaggata tctttttcca taccatgtgt gaacaccact tcttgccatt
    25081 ttatggtaga gcgcacattg cctacattcc agatggtcgt gtggcaggct tgtctaagct
    25141 agcccgtacg gttgaagttt attcgaaaaa accacaaatt caagaacgtt tgaatatcga
    25201 agtggccgat gccttgatgg actatctagg tgctaaagga gcctttgttg tcattgaggc
    25261 ggaacatatg tgtatgagta tgcgtggtgt tagaaaacca ggcactgcaa ccttgacgac
    25321 agtagctcgt ggtctatttg aaacagataa ggatctccgt gaccaagctt atcgtttaat
    25381 ggggctataa aaagaatccg cttcaagcgg atttttctag aaaggaatca ttatggatca
    25441 actgcagatt aaggatttgg aaatgtttgc ctatcatggt ctttttccta gtgagaaaga
    25501 attggggcag aaatttgtcg tttcagccat cctatcctat gatatgacca aggcagctac
    25561 agacttggat ttaacagcct ctgtccatta cggagaattg tgtcagcagt ggacgacttg
    25621 gtttcaggaa acgagtgaag atttgattga aacggtagcc tataaactgg tggaacgtac
    25681 ctttgagttt tatcctcttg tccaagaaat gaagttggaa ctgaaaaaac cttgggcacc
    25741 ggtgcatttg tcactagata cttgctcggt aaccattcat cgccgcaagc aacgagcctt
    25801 tatcgcccta ggaagcaata tgggagataa acaagcaaac ttgaagcaag ccattgacaa
    25861 actgcgagct cgtggcatcc atattctcaa agagtccagt gtcttagcga cggagccttg
    25921 gggtggagtg gagcaggata gctttgccaa tcaagtggtt gaggtggaaa cctggctacc
    25981 agcacaagac ttgttagaaa ccttgttagt cattgagtca gagctgggac gggtgagaga
    26041 agtgcattgg ggacctcgtt tgattgattt ggacttgctc tttgtggagg accagatcct
    26101 ttatacagac gacctcatat tgcctcatcc ttacatagcg gaacgccttt ttgtccttga
    26161 gtccttacag gaaattgcgc ctcattttat ccatccgata ttaaaacaac cgatccgcaa
    26221 cttgtatgat gctttgaaaa aatagaaaaa ctctagtttt cagttacttg caactgaagg
    26281 ctagagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtac
    26341 tcaagtacag cttgcggcta gcttcctagt ttgctctttg attttcattg agtattaaaa
    26401 taggtcattt tcttctggga ggaggatagt ttctctaccg tccatgtcta aaaccagtac
    26461 tcttggggga taacgagggt cgaaaggatg gttaaagtca aaatcaatgg ctgtagggag
    26521 gtgttgactt gaaaagtgga aggtaatctt tccttggtta ttaagcaatt gaaactcgag
    26581 ttcttcttcc aattcaaaga cattttttaa gaaatggtcg atgatatacc aaaaagagtc
    26641 aatgatgtca tcaggcaagc tggtaacaat accaaaacta gcagatcgca tgtgggtatt
    26701 ggtaaaagcc atatctctgt cccctttctt ttcccttatc atacagcaaa taggattaaa
    26761 aatcaagaaa aggtgatttt ttgaaaagga ttttagttac agggagaaat agggaaaaaa
    26821 ttcctaaaaa tctaccgaag ttaataggta aattcccaaa ttaacttgat tatataactt
    26881 tcagttactt tgagaagtta ccgaaaaata tttttcatat ctattgactt ttaggggtaa
    26941 aatttggtat gatagtaggc ggtattgttt accccatttg aaaggccccg gaaccttcca
    27001 aatacttttc gatgggaagg aacacccatc accgtaaaca aaaatcgaac tatatatagg
    27061 agaaatcatg aacaaaacaa catttatggc taaaccaggc caagttgaac gtaaatggta
    27121 cgtagttgac gcaactgatg taccacttgg acgtctttct gcagtagttg ctagcgtact
    27181 tcgcggaaaa aacaaaccaa catttacacc acacactgat acaggtgact tcgtgattgt
    27241 tatcaatgct gaaaaagtta aattgactgg taaaaaagca actgataaaa tctactacac
    27301 tcactcaaac cacccaggtg gattgaaaca aatctctgca ggtgaacttc gttctaaaaa
    27361 tgcagtacgt ttgattgaga aatcagttaa aggtatgctt ccacacaata cacttggacg
    27421 cgctcaaggt atgaagttga aagtatttgt tggagctgag cacactcacg ctgcacaaca
    27481 accagaagtt cttgacattt caggacttat ctaaggaaag gaacaataaa gtatgtcaca
    27541 agcacaatat gcaggtactg gacgtcgtaa aaacgctgtt gcacgcgttc gccttgttcc
    27601 aggaactggt aaaatcactg ttaacaaaaa agatgttgaa gagtacatcc cacacgctga
    27661 ccttcgtctt gtcatcaacc aaccattcgc agttacttca actgtaggtt catacgacgt
    27721 tttcgttaac gttataggtg gtggatacgc tggtcaatca ggagctatcc gtcacggtat
    27781 cgctcgtgcc cttcttcaag tagacccaga cttccgcgat tcattgaaac gcgcaggact
    27841 tcttacacgt gactcacgta aagttgaacg taagaaacca ggtcttaaga aagctcgtaa
    27901 agcatcacaa tttagtaaac gttaattcga aagaattact atacttatac agagcacctt
    27961 tcggggtgtt ctttttttat actttcttac taaattggtg caattgacac agttgttgcg
    28021 actttagtcg cttacaaatg tggctgcaac ctgacatggt cagttgcctc aaaacgttaa
    28081 tcaatacgat tatatcaacg tttcaaagca ctcaagggtt taccctatgg gtgctttttt
    28141 ctatactttc taaaaaagtt taccctaaaa tttgccctaa aattacccta cttattttta
    28201 agatgttggt aggcaacttg tccagcagat aatggaacta tgtttgaagt attaacataa
    28261 gtcttagttg taacggtatc gctatgagtt aatgcttcag aaatggcttc taagctcatt
    28321 cctgcttttt tagcaagtgt cgctcctgta tgtcgtaatt tatgcggtgt agcgtgtgtc
    28381 agttctttat gtcgctttct aatgcttttc attttattgt tcagatagtc ggcatgtaaa
    28441 ggcttgttga tgtttccttt tgtgtcgata tatgtaaaaa caaattgttc tggattactg
    28501 aggatgccaa actttgctag ttcatatctt tgttgttttt cccaagaggt aagaagttga
    28561 agtaaatcat tagaaataga gaaaaatagg atagcaaaag ctctatttca ggagaaaatg
    28621 aagcaaatat ttccataata aaacgcatat tatcaaggtt gttcaatacc tgatactatg
    28681 cgcttttctg attttaaaga ctgattgctc agtctctttt tcattataat tcagttacaa
    28741 tttaatctta tttgagaaag tgggtgatgc tattctctaa taagactatg atattaattt
    28801 ttgatagtac ttccagaaat tatagatccc ctttcatccc caatttctat tttcaagtgt
    28861 ccgtattata tagcgtattc gaaattcact atatcttgtt aggatttggg tagaatcatt
    28921 gataacagac tgttgcaaat ctaattagct gtatgattta tatttatcta tttttctagt
    28981 aaccaatcta taatgctttt cttctcgatt ccattgtaat tggctgttgg ctgaatcgta
    29041 tccattgtta atagatattt agggaattga tctttaatgg cttctagtgg tctaagttct
    29101 ctttctagtg tttctggagc aagtgttgtt tcactgacct gataataagc gtagtggcca
    29161 agatcagtta caacaacaaa atcaacctca tacttatcta aattaccaac atatacttgt
    29221 gaatatctac gtctcaattc caaatatacc atattttcca agatatgcct aatgtcttct
    29281 ttctggtctg gtaataagag atgtcgtaaa cctaaatcaa cgggataata tttttctaaa
    29341 cgttgcaata atgctctacc ttttacatca aaacgtggaa cggaataaaa aagtaaacta
    29401 tctgtcaaag ttgtcaaata attttccaaa gtattatggg atattgaaac attttggctg
    29461 actagggtat tgcgaatctt atttgttgat attaagctac ctgtactact gagaagggtt
    29521 cggacaatgc gctcaataat agtaggattt ggttttccca atctagtgac aatatcattt
    29581 aacagtatgg agttatatat tcctctgaga tagtcaattt tttcatcgta agatgatgtt
    29641 tgcaataagt aagggaaagc actaaagaga taattgttaa aaatttctgt tgtattcaga
    29701 ttctctgtga gagattgacc tgatagatat tcttcaaatg acaaaggaag aacctctatc
    29761 tcaacatacc gaccagtcaa gtttgttgct aattggctac tcataaagta ggcgttagat
    29821 ccagtcaaat agaggtctac atttgctaag atgaatagac tatctgctac cagttcaaat
    29881 ttttcaacat attgaatttc atctaaaaag atatagtatg ttttcttgct aactaattga
    29941 tcttttatat aagcgaataa tgtttgaaaa tgtcgcagat catagtaact caaatcttcg
    30001 aaattgatga atataatctg atcctcgtct accccagttg ctagtaactc ctctttatag
    30061 agttgaaaaa gcacagattt accagctcgt ctaactccac tcacaacttt gatgattggt
    30121 cggtcacgat gttttcttaa aaaatctaaa taatgtggtc ttttgatata gttcataata
    30181 gttacctcaa agatagtata atacaaatag atgttatttt caaatattta gaaataatat
    30241 ttaatttgtt ttattatttc taagtgttta gaaatatagt
//
LOCUS       DBDESG010000025        26543 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.025, whole genome shotgun sequence.
ACCESSION   DBDESG010000025 DBDESG010000000
VERSION     DBDESG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.025"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..209
                     /locus_tag="ACKB38_001058"
     CDS             <1..209
                     /locus_tag="ACKB38_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7979839.1"
                     /translation="RKSSKLKPLTAEDKAYNHALSKERSKVENIFAKVETFKMFSTTY
                     RNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(947..1207)
                     /locus_tag="ACKB38_001059"
     CDS             complement(947..1207)
                     /locus_tag="ACKB38_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979840.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(1266..1817)
                     /locus_tag="ACKB38_001060"
     CDS             complement(1266..1817)
                     /locus_tag="ACKB38_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979841.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1807..2004)
                     /locus_tag="ACKB38_001061"
     CDS             complement(1807..2004)
                     /locus_tag="ACKB38_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979842.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(2008..2802)
                     /locus_tag="ACKB38_001062"
     CDS             complement(2008..2802)
                     /locus_tag="ACKB38_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979843.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(3210..3635)
                     /locus_tag="ACKB38_001063"
     CDS             complement(3210..3635)
                     /locus_tag="ACKB38_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979844.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            complement(3764..4123)
                     /locus_tag="ACKB38_001064"
     CDS             complement(3764..4123)
                     /locus_tag="ACKB38_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGL7979845.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(4169..4375)
                     /locus_tag="ACKB38_001065"
     CDS             complement(4169..4375)
                     /locus_tag="ACKB38_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979846.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(4362..4523)
                     /locus_tag="ACKB38_001066"
     CDS             complement(4362..4523)
                     /locus_tag="ACKB38_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979847.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4881..5837)
                     /gene="lytA"
                     /locus_tag="ACKB38_001067"
     CDS             complement(4881..5837)
                     /gene="lytA"
                     /locus_tag="ACKB38_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGL7979848.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6213..7583)
                     /locus_tag="ACKB38_001068"
     CDS             complement(6213..7583)
                     /locus_tag="ACKB38_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGL7979849.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(7889..9055)
                     /gene="recA"
                     /locus_tag="ACKB38_001069"
     CDS             complement(7889..9055)
                     /gene="recA"
                     /locus_tag="ACKB38_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGL7979850.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(9110..10366)
                     /locus_tag="ACKB38_001070"
     CDS             complement(9110..10366)
                     /locus_tag="ACKB38_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGL7979851.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(10451..11467)
                     /locus_tag="ACKB38_001071"
     CDS             complement(10451..11467)
                     /locus_tag="ACKB38_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGL7979852.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNLLRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(11475..11993)
                     /locus_tag="ACKB38_001072"
     CDS             complement(11475..11993)
                     /locus_tag="ACKB38_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGL7979853.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(11983..12426)
                     /gene="tsaE"
                     /locus_tag="ACKB38_001073"
     CDS             complement(11983..12426)
                     /gene="tsaE"
                     /locus_tag="ACKB38_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGL7979854.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(12512..13132)
                     /locus_tag="ACKB38_001074"
     CDS             complement(12512..13132)
                     /locus_tag="ACKB38_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979855.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(13515..14378)
                     /locus_tag="ACKB38_001075"
     CDS             complement(13515..14378)
                     /locus_tag="ACKB38_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7979856.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            14571..14741
                     /locus_tag="ACKB38_001076"
     CDS             14571..14741
                     /locus_tag="ACKB38_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="HGL7979857.1"
                     /translation="MRKKRGIKKLVTFALLGVFMFSNTIPYQQFIQKNKQLEIRVQSQ
                     KKSNGLDVGKAD"
     gene            14844..15068
                     /gene="pneA1"
                     /locus_tag="ACKB38_001077"
     CDS             14844..15068
                     /gene="pneA1"
                     /locus_tag="ACKB38_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HGL7979858.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            15075..15263
                     /gene="pneA2"
                     /locus_tag="ACKB38_001078"
     CDS             15075..15263
                     /gene="pneA2"
                     /locus_tag="ACKB38_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HGL7979859.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            15379..18369
                     /locus_tag="ACKB38_001079"
     CDS             15379..18369
                     /locus_tag="ACKB38_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="HGL7979860.1"
                     /translation="MKYKIKNVHKTLFIKERYQILSERNDNSLNNFSLNNWRSTMSII
                     SEKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAKNSEWYKFAEGSFEIFRKH
                     PQRINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKNGNIFSSLVFYLEDLIYPW
                     IVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENILNFYNKYPVLLRQISESC
                     LRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKI
                     VYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKNLP
                     EYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDT
                     LFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKI
                     DFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTR
                     VLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDI
                     PIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSF
                     DQRKKGYKLNLSNRKKLPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGS
                     KWCLEQTGNDMYRGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGL
                     TGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINT
                     LVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKV
                     TRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGS
                     MVIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIA
                     YTFLRYIALNQNDKKIVIPSLLEI"
     gene            18719..19423
                     /locus_tag="ACKB38_001080"
     CDS             18719..19423
                     /locus_tag="ACKB38_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGL7979861.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            19404..20333
                     /locus_tag="ACKB38_001081"
     CDS             19404..20333
                     /locus_tag="ACKB38_001081"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HGL7979862.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            20330..22438
                     /locus_tag="ACKB38_001082"
     CDS             20330..22438
                     /locus_tag="ACKB38_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGL7979863.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVAS
                     LKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDL
                     LMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITS
                     EVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLT
                     LIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNT
                     KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSG
                     KSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTF
                     GHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKP
                     SVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEI
                     GTHRELLELGGIYKQLYSNN"
     gene            22462..24201
                     /locus_tag="ACKB38_001083"
     CDS             22462..24201
                     /locus_tag="ACKB38_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGL7979864.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            24252..25019
                     /locus_tag="ACKB38_001084"
     CDS             24252..25019
                     /locus_tag="ACKB38_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGL7979865.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            25021..25653
                     /locus_tag="ACKB38_001085"
     CDS             25021..25653
                     /locus_tag="ACKB38_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979866.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
BASE COUNT         8836 a         4601 c         4474 g         8632 t
ORIGIN      
        1 cacgtaaatc cagcaaactc aagccgctaa cagctgaaga taaagcctat aaccatgcgc
       61 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtagaaacg tttaaaatgt
      121 tttcaacaac ctatcgaaat catcgtaaac gctttggatt acgaatgaat ttgattgctg
      181 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattata tatgcacaga
      241 catatgaaat ctttttgaga tagcagttat gatatattta tccattcaca acatcttctg
      301 taccaatact acaatattgg tacattttga ttacacaatc gtttcaaaaa atatgatgga
      361 agcctgagta ttttatagta actcccttcc tatcaactag atcaaccagt tgagtgttga
      421 atgttgaaac accctagcct cagcaaagga ctatgagcag catttcctag ctaataggac
      481 gctccaaatc acctattcta aatccatcac tagcttggtc agtaagatac agactgcaag
      541 gcaagactga cgtggttgat gagattttgg aagagtatta caatcataga ttcaccagta
      601 gacttgaccg cagatttaac tatagtagat tgaaactaga atagtacact tctgcttcta
      661 aaacattgtt agaaatcgat ttgactgtcc tgatcaattt gtcctgttct cgtttcattc
      721 tactatacta aaaaaccacc tgaaacaaat caagtgacta gaggataaaa acgaagactg
      781 ggcaaaaagt tttgaaaagc aaaaaacgca taatatcagg tcttgaaaaa ccttgatact
      841 atacgtttga ttgtgggaag attgacttca ttttctcctg aaattgaagt tttttctagt
      901 ttccagatat agacgaattc aatatagcca tctcgctaca attattttaa ccatcgagaa
      961 aatttttcag aatttcaaaa atttcatttt gatccaatga aatgtcatta ttttgaagaa
     1021 tagttgactt cacaggctct aattctatca caaagcggtg catgtctgct tttaccgcac
     1081 tacttgatgt tatagccgat aacttttcat ctcctagcaa ggaagctgtc aaacgggtaa
     1141 ggtcattcaa atgcttttta atggacttac ttaaaccttg agcacctgtg gcagagcgct
     1201 ccctcatatc cagccaagct ttagccttaa aaataattaa tactcaatga aaatcaaaga
     1261 gcaaactagg aagctagctg caggctgctc aaaacactgt tttgaggttg tagatggaag
     1321 ctgacgtggt ttgaagagat tttcgaagag tataaaccac aattactcaa taccgaatac
     1381 ccctgaatgg tttctttttc atgcaccaat atattataat aatcttcatc caataataag
     1441 gctgataaac tagcatcttg gtcaaaatgt aagggaattt ctcgaccgtc cttctttaat
     1501 ggatattctg gtaagatact aaatagttca atcatagaag gaaactcagg attagttgtt
     1561 gtaaatcgaa aaagctgcgc tttctcatct ttctgacttc cttgatactc tcccaattct
     1621 aaaaaatgat tcaaggtagt ataaaattcc ttatttttta cttcatcaat gatgaccata
     1681 tcataatctt ttgttgtgcg acttttaaat ccttgcgaat ccaatacgat agaggtagca
     1741 gttcccccaa tcagaacata atagttctga aaatccgcaa acatttcttg aaaaataact
     1801 ttcgtattag ctggcatcat cttctcccag atactgtaat atcatatttt ctagtgcttc
     1861 actctcttcc tctatacgtg ggtcatcatc atcttttaag gtcaaataaa gagaaatcgg
     1921 atctacaaat tgtttatcat gattattttt aaaatcattc caaaactcag atacaaaagg
     1981 acgatatttc catatctcta gcatctttca ttttaaaaca tgctgagaaa gtggcaagga
     2041 taactgattg aattttctct gccatatgac atagctaata ttttcatccg tttcagctaa
     2101 aaaagttgaa tgcgacaaag cataagcacc accatataga aggttagaaa cagattttat
     2161 ctgctttata tcgccatctg gcaataaaat ccgttttttg atgggattaa ataaacatga
     2221 cacggatttt aagaataatt ctttctttga caccgtatat gtgtaaagct tattttgctt
     2281 gtttaaccaa tataaagctt taaaagtcct caaacaccta taaattgttg agtttggaag
     2341 tccagtgact tgtgaaagca aatcaacatc tactactttt tgaccttttg tcaataaaaa
     2401 ggcaatccac gttaattgtt cgctaggtgt taattcctta gggacttcag tatcattcgc
     2461 attgagtact agtcccaatg gagggaagaa gaggtttccc ttaaagtcta caaacggaac
     2521 tctagcttga agtaattgct ttttttcact gtctgataac ttcgaaaaca ccaaaacaac
     2581 atccatattg gctttttcac ccatagtgcg agcctgagta acaaatgaac tcaaactccc
     2641 ccttctcttt tctttaatca ataaaaatga ttccttatga aaagtcattt gagtatactc
     2701 aaaccttttt acaaaactga ttggtaaagt caaagtcagt tcctcaatac tgacatcaac
     2761 ataaaaggat tgaaaaattg acattatctt tttgacattc atgttttttc cttattctat
     2821 catttttatt atattttatc attcggtata ataaaatata agttttttga taaaataacc
     2881 aaaaactact cgctatcccc tctcacatta ccctacatat cgtttgacat gcgactgata
     2941 gttcaggaaa acttcaagga gaacttttct ctcatccact atgcaggact tactatttca
     3001 cttctactcc tataggctca atttgacact tttctttttc gaattgctca tatgcctcct
     3061 gaatagtgag ttcgatttat ccaaatttat ttttgtctat tttgatgaat acttccacga
     3121 agatagtctg aaaatgaatt tgcaccaatt ttccttctct ttttaagcat attattccag
     3181 atatgattgc cctcttattc agtaataaat tacgctttaa acgcttcaat cagtatatct
     3241 cctgttgtca tatattctaa agaaaattct tctacatatg atttaacatc tcttaggtta
     3301 ttacttccta atatcccatt atgctttttc gctaaggaaa tagatgcccc ttctccctta
     3361 ccaataatct tgttactatc atgatttctt gttaaatctc tatataatgc gtattcttca
     3421 gttccaatgt ctatgctcac aatctcagct gaacccttag ctaccaactg atctatccta
     3481 gattttaaat ggggaattgt aggtatattg atttcatcat acacctcttg tggaataaca
     3541 attttaccca aatagagctt ttctaaaaga tgttcagtgc caacccataa aaatactgaa
     3601 atacaatctg catcaataaa tacacgacta gtcaaacgac aactcccccc tcttcatcta
     3661 gcccatatac aatatcatat ctgaaagcat ctagtaacag ttcctcatac ttcccttgcg
     3721 aaattctgtt attttctaaa agttgttcag ttgaattaat atattatcct aatgccattt
     3781 cttttttact ttctgacaaa ggacgatata aacttgtatc atagcctaat cttgaagctg
     3841 tctctataac actaatatcc atatttttaa tttcttctgc atcaaggtat ccatcattcc
     3901 tcaatctata taacatagct ttatgactga taccataaaa ctgacccaat tttataatat
     3961 cttctacttc aagatgagtt ctattggcat tttctctgat ttcctcaacc atcctataca
     4021 gtgaagatgg gaaaattaaa aaataagaag caaactgatc cgcttttctt tcagtttcat
     4081 ctccttcacc aatcaagata agactgactg aactcttctt cacctcatca taataaagat
     4141 gatacagtcc atgtgctaaa gaaaatcttt accttcctaa tggcatgtct gaattgactg
     4201 caatgagact gaactgagtt cctttataac agactccgct aatattcttt ccgagtccat
     4261 aaaataccag cgtcaaattt tctatctttt gtaccaattt aaaaatatct atcggcgatt
     4321 caccatcagc tcccaacttt tttctaagat ttgaagcttt attacttaaa tccattcgtg
     4381 atattccttt cacctttttc cattttggca atcatgcttt gattcaaaga caaatactca
     4441 gcaatcttat cttgagtaag atttgaagat attctaagtt cttttattct ttttccaaca
     4501 tcacatacat ttatcacata catttatcat attcatacac ctctcataaa aagaatagca
     4561 cactcttgtc ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg
     4621 atgctgctta aaaatctaaa attgatgagt tcctatcaaa aatcgtatct tcaacctcaa
     4681 aacagtactt aaagctatcc gactcggttt acattgtcaa atttagattt tatttgagca
     4741 taactttcta gtttgctttt tgatttttgt ttaatatagt agcaaaattg aaggaaatat
     4801 ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg cgctgttctg
     4861 atttgaaaga cattccatta ttattttact gtaatcaagc catctggctc tactgtgaat
     4921 tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt tccgtccgct
     4981 gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta gtaccaagtg
     5041 tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata gtaccactta
     5101 tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca gtaccagttg
     5161 ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc aaagtagtac
     5221 caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga atgtacgtac
     5281 cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt ctcaatatca
     5341 tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg gtcaacgtgg
     5401 tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat tccagctaaa
     5461 ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg taagagttcg
     5521 atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc aatcagttca
     5581 accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc attatcaaca
     5641 ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc taattctggg
     5701 tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt cccagttgag
     5761 tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt tcttaattta
     5821 ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt tttacaatcc
     5881 agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc acgattaggc
     5941 ttgtagatag aacttcaaaa cgcactattt tgacactgta aataggactg acaaggtctg
     6001 cattctatct acaataacac cccagactaa aaagattttc aaaagtatat ttttacagtc
     6061 tctatatgtc cttttcataa atactatact ttattatatc atataaaaga agtcaaaagt
     6121 ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt cgattttcac
     6181 tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt tgccaagatt
     6241 gccttgcctt cttttatcaa gggatgacgg aataatgaga agtacagttg aactgtcatg
     6301 gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca aggaataatc
     6361 aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag aggaaggatc
     6421 ttttgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa aatgggataa
     6481 aaggttgaac tgatttgcag atagagactt ccattttcta tcaagtaacc atctgtcgaa
     6541 ctagccaaga aggaaaccaa agctggactg gcaaaaaaga ggaaaataca aacaaaaact
     6601 gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatacg gtcaggtcgc
     6661 ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc ggtcatagga
     6721 agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat cactgcacca
     6781 aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga actcatcaag
     6841 cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca tttgaaatgt
     6901 ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca gagaaccgct
     6961 gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag atgcaattgc
     7021 gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag aaatcccagg
     7081 gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata agcaaagctg
     7141 acacctacac aggtcacaat catagaaata tattgataag attgaggaag aatttctgca
     7201 ggagtatcta agtattgcaa gagaggatac aagccaagaa agcccagcaa cataactaga
     7261 atgctcaaaa gagcacctaa aatccaggtg gctgctactg cttccttgat tttagtgaaa
     7321 ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac accaagtgta
     7381 aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa ggattcttga
     7441 ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg aaaaatattt
     7501 gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc atgtatcagg
     7561 tccactgttc gttttttatt cataaagcta ttatatcagc tttccagagg agcgacaaga
     7621 aacagcaaac tatatatagc ggaatgaaat aaggaaagtc aaatcgattt ttaacaatgt
     7681 tttagaagta gatatgtact attctagttt cgatctatta tagttggttg agtttggaat
     7741 agtacgctgt agctgctaaa acatttctag aaattaattt gactttccta atagagttgt
     7801 tcatatctta tttcaagcta ctatatataa tctaaaaact tgaatcaaaa atcgaaaaag
     7861 tagcggattt ctccactgct ttaacagctt attcttcaat ttcgatttca agttcatcgc
     7921 ctaagtcaag cggaacttct tcattcactg cttcttcttt ctttggctca tcttttttgt
     7981 tttcagtatc ttgttctgaa acttcttctc catcaatcaa gccaaattta gaacggactt
     8041 gcttatcaat ttcatcaaag atttctgggt gctctgccaa gtatttctta gcattctcag
     8101 aaccttgccc aattttttca tctttgtaag aataccaagc ccctgctttt ttgataatat
     8161 ccaaatcgct tgcaatcttc aaaagctcac cagtcttaga aattccttct ccgtacataa
     8221 tttcaactac ggcttcctta aacggtggag ctaccttatt ttttacaacc ttaatcttag
     8281 tttctttacc gacattggtt tctttttggt caccagttcc cttaatttgt gtattaccac
     8341 gaacatccaa gcggactgaa gcatagaatt tcaaagcacg tccgcccggt gttgtttctg
     8401 gatttccaaa catcactcca actttttcac gcaattggtt gataaaaatg gcaattgttt
     8461 tggttttatt gatagaggcg ccaagtttac gcatggcctg gctcatcata cgagcctgca
     8521 aaccaacatg gctatctccg atatctccat caatttccgc acgaggaaca agggcagcaa
     8581 ctgagtcgac tacgacaaga tcaactgcac ctgagtcaat caattttccc gcaatctcaa
     8641 gaccttgctc tcctgagtct ggttgagaca agagcaattc gtcaatattg acaccaaggg
     8701 ccgcagcata agctggatca agggcatgtt ccgcatcgat aaaggcagca atcccacctt
     8761 ctttttgcgc ttgtgcaact gcatgaaggg caaccgttgt cttaccagat gactctgggc
     8821 catagatttc gatgatacgt cccttaggat aaccacctga gccaagggca atgtcaagag
     8881 ctaaagaacc tgagctcatc acttgcacct tttgctccgc acgttcaccc aaacgcatga
     8941 ttgatccttt accaaagtct ttctcaatca atttaagagc gtcattcaag gccttttcac
     9001 gttctgcccc aaattttttt gaaatttctt ctaatttttt tggttttttc gccattctat
     9061 tctcctacat tctaatgatc ctcagaccta tctactatta tatcaaaagt tagtcactta
     9121 ataaagcctt gcgaactagg ttaaaggcat gcataaccgc aatgtgacgt acatctgctc
     9181 ggcttctgcc tccaatatta accttgataa cctcagttcc ttgatcttgc gccaagccta
     9241 tgaagactgt cccaactggg tgtccttcta ggctatctgg tcctgccact ccagtcaaac
     9301 taattccaaa atcagactgg gtcttgcttc gtgcctgctc agccatcttc tgagctgtaa
     9361 attcagacac cacaccatgt tcttccaaat tcttggcagg aatatccaac atccttgatt
     9421 tttcctccaa gctataggtc acaaaaccac ccttaaatat acttgaaact ccagaaaaat
     9481 tcgccacggt agcttggaaa agacctgccg tcaaactctc tgcagccgcg atggttttcc
     9541 cttgcctttt cagttcttct accacaatgc tggctaaact agtttcttcc ccataaccat
     9601 agcaaaagtc tcgtaaagaa attccttcga aagtctggca gtccaagatt tgattttcca
     9661 agatatccag cgcttgattc gcctcttctt gactgctagc ctttgttgac agacgtagag
     9721 tgacttctcc tgtcttggca taaggggcca aggtaggatc gatctgatta tcaattaaat
     9781 cagccaaaat cgtaaccaac tggctctcgc caatcccaaa gaaacgaaga actcgggaat
     9841 acagcttgct ccctgtcatc aacttgggta gaagttggtt taagaccatg ggtttcaatt
     9901 cacttggcgg acctggaagg acgacatagg tcactccgtc tacttctaat tttcctccaa
     9961 cagccagtcc tgtttcgttt ggcagtggaa tcgctccttc tacaatttga gcttgtcttt
    10021 cgttattcgg tgttcgggca tagtctggtc gcagggtaaa aaagatatcc aacttctcct
    10081 gagcctgagg atcaaagact aatgctttcc ctaaaaattt agctagggtt tgtttggtta
    10141 ggtcgtcctc agttggcccc aaaccgcctg tcaaaatcac cagactgcta cgttgactgg
    10201 caatctcaag caaagacaag agacgaactt cattgtctcc tacagccgtc tgaaaatata
    10261 catctacccc aatctcagct agtttttccg acaaaaactg ggcattggtg ttgacaatct
    10321 gccctgtcaa aatctctgtt ccaacagcaa tgatttctgc tttcatgttt cctcctacct
    10381 atctattcgt atttttttga aaaaatcgca ggaattttcc tacgattgat ttttttattt
    10441 gtatcaaaag ttaattatct tcatcaccaa caggtgctct gccaaataaa tcttcaaata
    10501 aaaccgcatt ggtttcaagc tgagtaactt cttcttgtcc caaagaacgt cggagtagat
    10561 tttgcatttc caacatatgt gctctcgaaa caatctggta agaaacacct tgaagtatct
    10621 ctccttcacc ctgcaactgc tgagtttcaa tggttttaaa tgaatcttta tagcctagca
    10681 agttagggat actttttgca gacaaatcaa tattggtctg catattgtca ctcaaagctt
    10741 ttagaatctc ttgataatga ccaatgctat ttaaactgag agctttttcc atgacttttt
    10801 gaataacttc acgttgacgt ttttgacgac cataatcccc ctcaggatct tggtaacgca
    10861 ttcgtgcata gactagggct tcttctcccc caatatgttg ctccccaaca ccgatagaaa
    10921 tagtattaaa ttcttcttgg tcactgatag aaattgggaa acctaggata ttattgactg
    10981 taatacctcc tactgcatcc actagttttt gcaatcctct catattgacc atcacatagc
    11041 gatcaatatg gatattcatc attttttgaa tggtttctat agcaagctct gctccaccat
    11101 ctgcatatgc tgagttcagt ttcgcttcat gagcctgacc attccctgat tcaatgcgcg
    11161 tcagaatatc ccgctctaaa ctcatcattg ttgttttttt cgttttagga ttcactgtca
    11221 tcaagatcat gctatcactt ctaccgaccc aagtttcagt tcgttcaaca tttccggtgt
    11281 ccactcccat taacagaatg gttagaggtt cagtcgcttc aataaccttg gtttcttcac
    11341 cgattttttt ataggtttta gctaaggttt ctgtcccttg ttgataaata gtataagcaa
    11401 aaacacctac tcctactaca gttacagaaa gtaaagctag caccattcca ataatttttt
    11461 taaccatatt tctactaacc tatcagttta cccatcaagt aaacatcgat aaatttccct
    11521 tcttctatat atgccccacg ctcttggcta ccttcaatga caaagccatg cttttgataa
    11581 agatggactg ctgcttgatt acgagtttgg acagtcagtt ggagacgacg cagaatgcca
    11641 cttgcttgtg cccactctat cgcttcttct agcaacaaac ttcccaagcc attattccaa
    11701 tatctttttc caatcacaat gaagagatct ccaatatgac ggactctctt acgctgatca
    11761 gctgtaatat ttacaatacc agcaattttg ccatttaaga atgcaagtaa ggttatctga
    11821 ttgtccgaac tagcttgctt gttgaggaat atttccatct cctcactagt caagagaata
    11881 ccatctccgt ctaggctggt aaagtctgtc tccaaactca cacgatttaa aaaggccact
    11941 aattcagctg catctttggg ctctgcttcc ctaatgagca attcatactc catattgaag
    12001 ctcctctaac aatttctcag cacgcaaacc ctttgcctga aaatttaaac ggcgtccatc
    12061 tgcttctttt agaatttcca attctaaata agcatctggc aaggcatctc ctaagagatt
    12121 tccccactca ataacagtca cgccgccacc aaagataaac tcatccaagt cgatagaatc
    12181 agcatctcct tcaatacgat aaacatctag gtgataaagt ggaagtcgac cttcatactc
    12241 tctcacgata gtataggtgg gacttttaat catttgagaa atctgtaatc cttttgcaag
    12301 tcctttagta aaggtcgttt tacctgcacc cagttctcca gttaagatta aaacatcatt
    12361 ctttgctaat agatggccca aacgctcccc taaggcttgc aactcttctt cattttttgt
    12421 gtacatactc ttattatacc aaaaactttt cttttgtgtc tattttccta ctaaacttat
    12481 catcataaca tccataaaaa acaggctttc tctaaaagaa aatgagcgta acaatgacca
    12541 atacaagatc tcggaaaata tgaccataaa aggaaacttc cttcttaacc gaatttggga
    12601 caagataggc tgcaaaaaac aagcccagtc caatataaat cagaagtgag acaatggtca
    12661 ttggatttct taagaaaaga agtgttgcta aaatagtcac caacactgtc ttttttctgt
    12721 ccagcatagc aagaaaatcg cgcacgtatt ttttcaaggg taaaaaaatc agcaaatcta
    12781 gcccaaatag gaaaaagaag gatggcaata aaaagtcaac taattcttgc tgcagcgtat
    12841 ttttgatgaa caagttatct gacaaaacaa gaacagctcc taacaaatta attaagagta
    12901 acatactgta aaaaagcttc accgacttct tactggctag gacactatgg acttcttgct
    12961 tacgggtata aagataattt actccagcac agattcctga aacgaaaacc atgcttccga
    13021 tgaaaaaagc tgtactttgt ttaaaggaca agatgcattc cttccatagg aaacagctac
    13081 tcaaactgat ttgaattaaa gctaacaaaa ataagattct cattgatttc atcttctctc
    13141 tcccttccta ccaatcatta tactaggaga aaagagagaa ctgtttctaa tcttctcaaa
    13201 tgtctcttta agacgctaaa caaacactag agactaatac tcaatgaaaa tcaaagatca
    13261 aactaggtag ctagccacag gttgctcaaa acagtgtttt gagattgcag atagagctga
    13321 cgtgatttga agagattttc gaagaatata aatttgaaat catgaaaatc cgtcaaacgg
    13381 gtggttgttt tgtctcgcac ctcacggagc gagacggact cagagtcaca taattataag
    13441 gctgatagta ttaatctaac tatcagcctt acaggttatt taacgtttca gaaaaactat
    13501 aatgtcaaga ttaactaaac agtatctagt tccttcaaat aattttctat cttcatcaac
    13561 attaaaggat tgttataaat cttacataac tctcttgctt ctatataata atttttgact
    13621 tgttctctgt ctagaaattt ggctccagca tttcctacaa gaataagtag aggagccaat
    13681 tggtagcttg tctgtctttg tttacagagt tcaatcgttt caagagcttc ttggatggct
    13741 tcattatatt tttcctttga tactaggtag tgagcgtagt tgtaacgaac tctgatgtag
    13801 ccaaataaaa actcttgatg gtccaaattt tttgtctgat acaactctat taaatgagag
    13861 tagtttgcct catattcttg ttcacgaccc actaaggaat agaaattaga tagagtattc
    13921 aacgccttta aataaatcag agtatttgaa gagactttta ataatatatt ttccaatgac
    13981 gaaattgcct cacacttact gtcatattga tagaagtcaa ttatagattt aatccattca
    14041 aggtaagttc ggtcttctaa tgttagaaaa gtgcttcgtt ctacttctat tttataaaga
    14101 tattctaaat cgtcataatt tctgtcatct aataggcgag cagatagatg tttgaaatta
    14161 gagaggttag acttaacttc gatttgttca ttgaaaaagt aatccaaagg gacttcaagt
    14221 cgttgagaga gtttgaataa caagtctgcg gagggaataa aatgacctct ttcaatttta
    14281 ctaatctggc tttgttcaca aattccttct gcaagagttt gttgggagag tctcaactct
    14341 tttcttttca atctgaattt ctctgcgagt gtattcatta aagataataa accttcctat
    14401 ttgcttaatt tcattataaa gtttttagtt caaaatagca agttaaagga ataaataatt
    14461 ctttatggta ataaacaaaa aaaaaaagtt aaaaattaca taaaaatact tgactaaata
    14521 aaatatatct gttagaataa aaacaaggaa aaagaaaggg gtttcattgc atgagaaaaa
    14581 aacgtggaat taaaaaatta gttacatttg cattgctagg tgtttttatg tttagtaata
    14641 caattcctta ccaacagttt attcagaaga ataaacaatt ggagattcga gtgcaatcgc
    14701 aaaagaagtc caatggtctt gatgttggga aggcggatta aaacccgata ttttgcacga
    14761 gaatgagaga attataattt atctgaaaaa acgaaaaata tagagcaact tatttaactt
    14821 tttataagga ggagttatta cttatgacca actttaattc aaacgaaaaa ttttgtggaa
    14881 aaagtttaaa atcattgtct gctgatgaaa tgagtttaat ttatggtgca tctgatggtg
    14941 cagagccacg ttggacgcct actcctatta ttttgaaatc agctgcagct agctcgaaag
    15001 tatgcattag tgcagctgtt tctggtattg gggggctagt tagctacaat aatgattgtt
    15061 tggggtaaat taaaatgaaa aatgattttg taatcggaaa gagtttgaaa gaattatctc
    15121 ttgaagaaat gcaattggtt tatggtggca cagatggtgc tgaccctaga tcaacaatta
    15181 tatgttctgc tacattaagt tttattgcat catatttggg gtcagctcag actcgttgtg
    15241 gcaaagacaa caagaaaaaa taaaatattc aattttaaaa ataaaagttg ctctttattt
    15301 tcctctctcg taactaataa ttttgagaga ggtttttttt taaaacaata gtaagtcgta
    15361 taggtttgag gtaagtaaat gaaatataaa attaaaaatg tgcataaaac attgtttatt
    15421 aaggaaagat atcaaatatt atctgaacga aatgataatt ccttaaataa cttttcatta
    15481 aataattggc gttctacaat gtcaattatt tctgaaaaaa cttttgaaga aatgttgaaa
    15541 ttaaataatt acaataaaaa aatattttct aacactgtga actatgatta taccgaggaa
    15601 cagataagac tatattcaga atatgccaaa aattcggaat ggtataaatt tgcggagggt
    15661 tcatttgaaa tatttagaaa acatccccaa agaattaatt ttaagaaaga tttttctaga
    15721 attttggaac cctttattat taatttgctt aaattaatca ataattatct agagaataaa
    15781 gaatacgagt ggattgacaa gaatggaaat attttttcct ctctagtatt ttatttagaa
    15841 gatttaatct atccttggat tgttaaacct ttggttttag agataaattc attgcgtgaa
    15901 aaaggtttac ttgaagggga atcggagcag caacggtaca aatattttat aacattgttt
    15961 gacaaggaag agaatatatt aaatttttat aacaaatatc ccgttttact gaggcaaata
    16021 tcggagtctt gtcttcggtt ctatacttat tttatagaaa ttttatcaaa tttagaaaat
    16081 gattttagtg tgctagaaga agaattaggg ctaaggggga aattaaatga tataaaattt
    16141 ggaaagggtg atacacacag ccaaggaaaa actgttttga tactcttctt tgatgacgcg
    16201 aaaattgttt acaagcctaa aaatttaata atcaataact cactaaatac tattgctgag
    16261 tatatccgaa aggttgatga aaaaattagg ataagaatac ctcgaactat tgcttattcg
    16321 gatcacagct atgaagaatt tattgattat ctacctctag agcaaaagaa aaatttacct
    16381 gaatattatt ataattttgg tgtgctttta gcatttatat atttatttaa tgggagtgat
    16441 atacattttg aaaatttaat ttcctatgga gatatgcctg taataataga ctttgaaaca
    16501 atgttacagc aacctctatt tgatgataaa actggtcaga gtttgctgga taccctgttt
    16561 cacagggtga ctcgaacttt gcttcttcca acagagggag taaaaaggga ggatggtctt
    16621 gatgttgaga tgagtgctct tacaggaaat tttaagaagg atgcttttaa tggtcaagtt
    16681 ttaattaatt taaatactga taaggtaaag tttgatattg ggaaaattga ttttgaaggt
    16741 gggaaaaacc tgcctgttcg ggatggtgat atagaatttg ataagtatat aaaagatttc
    16801 aaaaaaggtt ttagggattt ttatctgata tttgaagagt taaataaaac agaagaattt
    16861 aaaatgctat taaaagcaaa cttgtatggt ttaaagacaa gggtactttt tagagacaca
    16921 aatagctatg cttcagtact atctttttta tatcatcctg atttttatga agaaatgtta
    16981 gatagagaaa aagcattgga aaatctctgg agcaataagt tttctaatca aggtattgta
    17041 gcaagcgaat gtgaacaaat gaggttatta gatattccga ttttttatac agatacaaat
    17101 atcaatgaaa tctacgatga ttttggaaat cattttattt ccctacatca attttctgga
    17161 tatgactatt ttatgaaata tttacctaat aataatttgg aaaatttaga aaagcaactt
    17221 gatatcattg aaatttattt tccaagcttt gatcagcgaa aaaaaggata taaattaaac
    17281 ttatctaata gaaaaaaact cccaaatgaa agtttagtta ctattctgca taatgaagta
    17341 gaaaatattg cagactcttt ggttattaat aatatttatg acgaatatcc aatttggagt
    17401 atacctgtta attttaaagg ttctaaatgg tgtcttgaac aaacgggtaa tgatatgtat
    17461 aggggtagag ctggaatatt actgtttatg tactatgtaa aattattgaa agataaatca
    17521 tcatatactc aactgtacaa taaaatgtta tctgcaattc cgccatctgt ttctttatct
    17581 aagaccacat ttggtttaac tggggatata ggatatttca ttgttctatc cattatagag
    17641 gattacgata caaatactct cgcatgctta aattatataa agagtgtttt aactgagctt
    17701 gaaaagactg attttgcaag tgtttcaaac aagtctgact atattaatgg tcttctacct
    17761 ttaattaata cactggtaag atattatagg agaggaatag agaaggaacg tgttctaagg
    17821 ctagtattaa gtttaggtga tacgctatat aatgatgtaa gcgagagaga taatagtaat
    17881 tttggatata gttttggcca tggactatca ggtgtaattt ttacattaaa aaatatttat
    17941 aaagtgacaa gacttaaaaa atataaagaa ttaattttac aactagttcc tagggagaaa
    18001 atcaaaattc attctttgaa gtggtgtagt ggagaatttg ggacattagt taatcatccc
    18061 caaagtgttc cagagggaat tgtagctagt ttagataatg atacattttg ccatggaagt
    18121 atggtcataa taaattttta catcgactac atgaaagaaa tggatactag tatggaattg
    18181 gagtctttat tgtgtagtgt tgtaatgaat aaatcaaaag aaggagagta tcgtatcgtc
    18241 caaacaagca atttcccaga tttttctttg tatacaggtg tgactggtat tgcttatact
    18301 tttttaaggt atatcgcact gaatcagaat gacaaaaaga tagttatccc ctcattactt
    18361 gaaatctagc ttaatttata gttttgtttc taaaatagag gtgtccactc attaagcaat
    18421 ataaaaatac caagattgac tagtttaata tgtaattcta taaaatattt ttaatagtgt
    18481 agcttttcgt tgagataata atataataaa gaaaagttct attctaattg ctaaacggct
    18541 tgaggtatat gctactgtta tagaaatgat tttaaactaa aatcaaaatt actccaaaaa
    18601 tagtattgat tttggtttaa tacaaaacat atgtaataat gtgagtcaac aaaagtctgt
    18661 ttttgtacca tactatcttg aaaaatctac taaattcaaa ttgagaggat gaattaaaat
    18721 gaataaaata tttatatatg caggagtgcg aaatcataac tctaagactt tagagtatac
    18781 taagagactt tcaagtatca tttctagtag aaataatgtt gatatatctt tcaggacacc
    18841 tttcaattca gagctagaaa tatctaattc tgattcagaa gagttgttta agaaaggaat
    18901 tgataggcaa agtaatgctg atgatggagg tgttataaaa aaagagttat tagagtctga
    18961 tattataatt atctcttctc ctgtatattt acaaaacgta tctgtggaca caaaaaactt
    19021 tattgaaagg attggggggt ggagccatct ttttcgatta gctggaaagt ttgttgtaac
    19081 attagatgtt gctgagagta acggctctga taatgtgtca gaatatctta gagatatatt
    19141 ttcatatatg ggaggacaga tattacacca agtttcaatt accaattcac tcaaagatat
    19201 tgcagaggct cagttgatgg aggcaactta taaaattgaa gatgtacttg aagggaaaat
    19261 taagtacaaa acaacggatt atcaagaaag agcttaccag acattaaaat taattttaga
    19321 aaattatgat tccgaacatt ttgagaagat gtattgggag aaaaaacgcc tttttgaagc
    19381 taatagttta gaggaatggt attatgttga aaatattaaa taactctgtc atatatttat
    19441 gtttgattcc gttattgttc ctattattga taatttttcc aaatgattca ttaatctatt
    19501 attttcgatt gattttaatt tcgttaatat caattacatt acatgaacta ggtcattttt
    19561 tagtgggaag atgtttatct tataaactag agatgcttgc tactcccttt ttcttctatt
    19621 tcagaaaaaa aatatatttc aaatttccag tgttattagc attcggttat tgtcaaatga
    19681 gtaatagaaa cattactaat gaaaaaaata gtgataggaa tttggtgttc tacttctttg
    19741 ggggaggtgg agctaaccta attgtcgcaa tattagcact tttgggattt gtgccgtacg
    19801 catcagaatt ttttattttg aatataattc tttttctagt tacggtatgt ttaccaattg
    19861 atggaacaga tggcaatgct attagagaga tagttttgta ttcaaaagat tcaaaaacat
    19921 atcagagatt ctttgcgaac agtctctata ataatcctta tataacaatc gacgattttg
    19981 caaaattaac ctctaaagaa aaatcttttt tttcaaaatt tgaaaaatgt ttgataaacc
    20041 tttactttga agtgaaaggt gaagggtcag catttacaat tgctaatcat gaagtttttg
    20101 attcaaatat tcaggaaaat attatccatg agtactataa attactacat aaatcttctg
    20161 attggattat tccacagaac ttatctttag aagaagttgt ttttaccttg tcaaactata
    20221 tttactcgaa aaataataaa tatttagaaa agattaagta tctaaagaaa ttagtagatt
    20281 ttagacaaga agaaattatc gactttattt tgaacaagga ggaagtattg tgaaaattcc
    20341 aatgatatat caaatggaaa attcagaatg tggattagca tgctgtgcca tgatattgaa
    20401 ctattttaaa tatgagattt ctttaaatga actacgtgaa atctacccat catccagatc
    20461 tggatattct ctcctatcta taagtaaagt tttaggagat tttaatataa gttctcatgc
    20521 ttttaaagct tcggtaagag atttaaaacc gctcagtttc ccactcattt gcttctggga
    20581 gagttctcat tttattattc ttgaaaaaat tagtaaaaac aagttttata ttttagatcc
    20641 tgcaaaaggc aggcagagaa tgtcaataag tgaatttgaa aggcattatt caaatatcat
    20701 tttaacattt aaaaagttag atagctttat gtctcgtaaa gataataaga agtcgcctgt
    20761 tttaaagtat ttttttaagt ataggaataa gctagggatt ttattttttg taacagcatt
    20821 attgtatgta atacaatcat tagtacctat agctaataga tacataattg acacgaattt
    20881 caaggacgat tcgtattcgt ctagaatgtt atttactata ttatttatat ttactgtttc
    20941 attctcacta atgtatttat taagacagat atatgttgca tccttaaaat atataatgga
    21001 taaagagatt agctatgatt ttatgaaaca tttgatatat ttaccttaca gtttttatga
    21061 aaaacgtact ttaggggata tactttttag agctaactct attgtttata taagagaaat
    21121 actatcaaat aattttatag cagctatact tgatttgtta atgattgtgg tttatgctgt
    21181 ggttttattt agcttttcta agtacatggt aatcttttta atatcactaa gtctagctct
    21241 atctattgta atgtatccaa tcataaaaat ctcaaaaaat ttaattgata aaaatataaa
    21301 agaaaaggtt aatgttcaaa atattacttc cgaagtaatt tctaaaaata gtgatattaa
    21361 gctaactgga gaagaggaat tttggattaa caaatgggat aattttaata caaaacagct
    21421 catcataggt cgaaaacttg atatacattt atcaattgtt agtagtataa cgaatgtttt
    21481 acaaattatt ctccctgttt tgacccttat tgtaggtgta aatataaaaa cattcgaaca
    21541 attgacgtta ggacaaattg tagcaataag tacagtctca ccatacttta tttctcctat
    21601 aatttcttta agtgataact atatacaatt aatgttatta aagggatatt ttttaagaat
    21661 agaggatgtg tttaatacta aatccgaatt aattccagaa agagtcagtc aagatataaa
    21721 atttgataaa aaaatagaat taaaagatat ttggtataaa tatggattat ttgatgatta
    21781 tgttttgaaa ggaataaatg ttactattaa aaaaggagaa actgttgcta ttgttggaga
    21841 atcaggttca ggtaagagta cattagctaa aattttatta ggtttattag aacctaatat
    21901 tggttcaata gaagttgatg gagtagaaaa agaagaaatt ggtcaaacat tgtatagaaa
    21961 gatttttgga gcagtgttac aaaattcaac cctaagttat ggtaccttaa gagagaattt
    22021 gacatttgga cactttgttt cagatgaaga attaatgaca aatctaaatt caattggtct
    22081 tagcaatgta gttaaatctt tacctcttgg attagagaca atcatcgctg aagaaggtaa
    22141 taacttttct ggagggcagc agcaaatgat acttttagct cgttgtcttt tgtcgaaacc
    22201 ttcggtagtt gttttggacg aagcaacaag tagtttagat aatttatctc aacaaattac
    22261 aacttcttac ttaagtgaaa tcggtaccac taagatttta attgcccatc gactagatac
    22321 tatcaagtct gcagataaga tcttagtaat gcataatggt gaaattgtag agattgggac
    22381 ccatagagaa cttcttgaac taggaggcat ttataagcaa ttgtattcaa ataattagtt
    22441 tttgattaaa agggtaaatt tatgaagatt atgaaaaaaa aatattggac tttagcgata
    22501 ttattctttt gtttgttcaa taattctgtt actgctcaag aaatacctaa aaatcttgat
    22561 ggcaatataa ctcacactca gactagcgaa agtttttctg aatctgatga aaaacaggtt
    22621 gactattcta ataaaaatca agaagaagta gaccaaaata aatttcgtat tcaaatcgat
    22681 aagacagaat tatttgtaac aacagataaa catttagaaa aaaactgttg taaattggaa
    22741 cttgaaccac aaataaataa cgatattgtt aactctgaaa gtaataattt actaggcgaa
    22801 gataatttag ataataaaat taaggaaaat gtttctcatc tagataatag aggaggaaat
    22861 atagagcatg acaaagataa cttagaatcg tcgattgtaa gaaaatatga atgggatata
    22921 gataaagtta ctggtggagg cgaaagttat aaattatatt ctaaaagtaa ttctaaagtt
    22981 tcaattgcta ttttagattc aggagtcgat ttacaaaata ctggattact gaaaaatctt
    23041 tcaaatcact caaaaaacta tgtccccaat aaaggatatt taggaaaaga ggagggagag
    23101 gaaggaataa tatcagatat tcaagataga ttaggtcatg gtacggctgt tgtagctcaa
    23161 attgtagggg atgacaatat taatggagta aatcctcacg ttaatattaa cgtctataga
    23221 atatttggta agtcgtcagc tagtccagat tggattgtaa aagcaatttt tgatgctgta
    23281 gatgatggca atgatattat caatcttagt actggacaat atttaatgat tgatggagaa
    23341 tatgaggacg gaacaaatga ttttgaaaca tttttgaagt ataaaaaggc tattgattac
    23401 gcgaatcaaa aaggagtaat tatagtagct gcattaggga atgactccct aaatgtatca
    23461 aatcagtcag atttattgaa acttattagt tcacgcaaaa aagtaagaaa accaggatta
    23521 gtagttgatg ttccaagtta tttctcatct acaatttcgg tcggaggcat agatcgctta
    23581 ggtaatttat cagattttag caataaaggg gattctgatg caatatatgc gcctgcaggc
    23641 tcaacattat ctctttcaga attaggactt aataacttta ttaatgcaga aaaatataaa
    23701 gaagattgga ttttttcggc aacactagga ggatatacgt atctttatgg aaactcattt
    23761 gctgctccta aagtttctgg tgcgattgca atgattattg ataaatacaa attaaaagat
    23821 cagccctata attatatgtt tgtaaaaaaa attctggaag aaacattacc agtaaaaaat
    23881 ggtataaaag tgttaaatat accaaacgta ttgagatatg atttgaatat gttacaatta
    23941 gaatataaaa atgaacaaag ttgggatagt ttcatagata atgttaattt aattgagttg
    24001 gaagagagaa ttcaaactac tattggaatt aaacaaataa acacacacaa tattattact
    24061 attgcccgag aagggtactc tcaaaattat ttacctaaca cttcagaaaa tacatataat
    24121 tcattacaag tcagtttagt tggagtatta ctacttttta taagtatggt aaatatttta
    24181 tgggctaaaa aaagtaaatg aaaataaaat ttggagccct ctgaaaaagt aagtcctaca
    24241 gttcaactaa aatgagtcaa aagatgaatc accttgatgt aggggagttt gtcttattgc
    24301 tgcctgaaca cctccgttca gaggaagaac attataaatc tgtttttgaa gacgacttaa
    24361 ccagtcgcat atctagtcaa gatgaacgac agcaaatgac tgctacggta ggttatttag
    24421 aatcaggtca ggatcgtttt gtgtataata cgacccctat ttcttaccag cagtttttga
    24481 aagatccaat catcattgtt ataacacccc aatcaactgg tccacagtcc attttgtttt
    24541 ggatagacgc agtacagaac tacgttctct ttaatcaatt gtctgatgcc caggagctta
    24601 tccagagaca aggcattgaa aattgggtct cagaaatgca aacaggttac cacaactaca
    24661 tcacattatt ggataatatc cagagggaac gttgggtaat gctagcagga gctgtgcttg
    24721 ggattgcaac ttcaatcttg ttgtttaaca ctatgaatag gctctacttt gaagaattta
    24781 gacgtgccat ttttatcaaa cgcattgcag gtctcaggtt cttagaaatc catcgcactt
    24841 atctctttgc tcaactgggt gtgtttttac tgggatttgt tgcgagtgta tttcttcagg
    24901 tagagatagg agttgctttc ttagtcttgt tactctttac tggtctatct cttttacagt
    24961 tacatgtcca aatgcagaaa gaaaacaaga tgtccatgct tgttttgaag ggaggttaat
    25021 atgattgaac ttaaacaggt gagtaaatct tttggagaac gagagttatt ttcgaatctt
    25081 tcaatgacat ttgaggctgg aaaagtctat gccttaattg gttcaagtgg tagcggaaaa
    25141 acaaccttga tgaacatgat tgggaaatta gaaccttatg atgggacgat tttttaccga
    25201 ggtaaagact tggccaatta taaatcaagt gattttttcc gtcacgaatt gggctacctc
    25261 ttccagaact ttggcttaat tgaaaaccaa agtattgaag aaaaccttaa gctaggtctc
    25321 attggtcaaa agttgagtcg gtcggaacag cggttgaggc agaagcaggc tttagaacag
    25381 gtcggcctgg tttatcttga cctagataag cgcatctttg agttatcggg cggagaatcg
    25441 caacgggttg ccttggcaaa aattatctta aagaatccac cctttattct ggcagatgag
    25501 ccaacagctt caatagaccc agcaacctct cagttgatta tggagatttt gctatctctt
    25561 cgagatgata ataggctaat cattatcgca acacataatc cggcaatttg ggagatggct
    25621 gatgaagtgt tcacgatgga tcatctgaaa taaaaatcct tgtttttaat tgcacgatga
    25681 gttactgaaa tattatcatg aatcaagaat tggagttaat ttagaattgt acttaattta
    25741 gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaagaa ataatggaga
    25801 ggaagttaga atgaaaaaat tcgacaatta tattattgag aagccttgcg attctaattc
    25861 agataaactg caaaaaatct taataattga aagtttggta gatgatattt tgcaattttc
    25921 tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttt aaaagaaaac
    25981 tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg atgataaagt
    26041 tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt aatctgtgtt
    26101 gaaggctcaa aacctatggt aaaaaagtag ctttgaaaac gtattgcctc caaagattta
    26161 gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat gttgtttcag
    26221 tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa aaagtataga
    26281 aatatagtca attgaaacaa gaacaggata aaagaacctt ttgtgccata tttttctcct
    26341 ttcgctttac aattggattg aacaccttta ttgtatcgcg tttggagttt ttttggtata
    26401 accttcgacg cacacccgca tagcgggtgt tttttttgtc tcgcacctaa cggagcgaga
    26461 caaactaata gtcacttaat caaaaaacgc accatatcaa aaactaaaaa gtttgatatc
    26521 atgcgtcatg tcttaaacta att
//
LOCUS       DBDESG010000026        24556 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.026, whole genome shotgun sequence.
ACCESSION   DBDESG010000026 DBDESG010000000
VERSION     DBDESG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.026"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..722)
                     /locus_tag="ACKB38_001086"
     CDS             complement(<1..722)
                     /locus_tag="ACKB38_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979867.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYSNSSCICTSSGTSF"
     gene            complement(781..1731)
                     /gene="murB"
                     /locus_tag="ACKB38_001087"
     CDS             complement(781..1731)
                     /gene="murB"
                     /locus_tag="ACKB38_001087"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGL7979868.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(1843..2595)
                     /locus_tag="ACKB38_001088"
     CDS             complement(1843..2595)
                     /locus_tag="ACKB38_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HGL7979869.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(2605..3336)
                     /gene="budA"
                     /locus_tag="ACKB38_001089"
     CDS             complement(2605..3336)
                     /gene="budA"
                     /locus_tag="ACKB38_001089"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGL7979870.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHRAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            3478..4749
                     /locus_tag="ACKB38_001090"
     CDS             3478..4749
                     /locus_tag="ACKB38_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGL7979871.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYSLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(4769..5584)
                     /locus_tag="ACKB38_001091"
     CDS             complement(4769..5584)
                     /locus_tag="ACKB38_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7979872.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVVQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(5741..6394)
                     /gene="phoU"
                     /locus_tag="ACKB38_001092"
     CDS             complement(5741..6394)
                     /gene="phoU"
                     /locus_tag="ACKB38_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGL7979873.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(6406..7164)
                     /gene="pstB"
                     /locus_tag="ACKB38_001093"
     CDS             complement(6406..7164)
                     /gene="pstB"
                     /locus_tag="ACKB38_001093"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGL7979874.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(7177..7980)
                     /gene="pstB"
                     /locus_tag="ACKB38_001094"
     CDS             complement(7177..7980)
                     /gene="pstB"
                     /locus_tag="ACKB38_001094"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGL7979875.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(7991..8875)
                     /gene="pstA"
                     /locus_tag="ACKB38_001095"
     CDS             complement(7991..8875)
                     /gene="pstA"
                     /locus_tag="ACKB38_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGL7979876.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(8865..9782)
                     /gene="pstC"
                     /locus_tag="ACKB38_001096"
     CDS             complement(8865..9782)
                     /gene="pstC"
                     /locus_tag="ACKB38_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGL7979877.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVHNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(9782..10660)
                     /gene="pstS"
                     /locus_tag="ACKB38_001097"
     CDS             complement(9782..10660)
                     /gene="pstS"
                     /locus_tag="ACKB38_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGL7979878.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(11234..12538)
                     /locus_tag="ACKB38_001098"
     CDS             complement(11234..12538)
                     /locus_tag="ACKB38_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGL7979879.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLWKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(12539..13312)
                     /locus_tag="ACKB38_001099"
     CDS             complement(12539..13312)
                     /locus_tag="ACKB38_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGL7979880.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(13302..13580)
                     /locus_tag="ACKB38_001100"
     CDS             complement(13302..13580)
                     /locus_tag="ACKB38_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGL7979881.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(13580..13981)
                     /locus_tag="ACKB38_001101"
     CDS             complement(13580..13981)
                     /locus_tag="ACKB38_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGL7979882.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(14191..14808)
                     /locus_tag="ACKB38_001102"
     CDS             complement(14191..14808)
                     /locus_tag="ACKB38_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGL7979883.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(14798..15571)
                     /locus_tag="ACKB38_001103"
     CDS             complement(14798..15571)
                     /locus_tag="ACKB38_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGL7979884.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(15582..16319)
                     /locus_tag="ACKB38_001104"
     CDS             complement(15582..16319)
                     /locus_tag="ACKB38_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGL7979885.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(16324..17454)
                     /gene="hemW"
                     /locus_tag="ACKB38_001105"
     CDS             complement(16324..17454)
                     /gene="hemW"
                     /locus_tag="ACKB38_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGL7979886.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(17538..17927)
                     /locus_tag="ACKB38_001106"
     CDS             complement(17538..17927)
                     /locus_tag="ACKB38_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGL7979887.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQTKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(17900..18326)
                     /locus_tag="ACKB38_001107"
                     /pseudo
     CDS             complement(17900..18326)
                     /locus_tag="ACKB38_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            complement(18327..19115)
                     /gene="lgt"
                     /locus_tag="ACKB38_001108"
     CDS             complement(18327..19115)
                     /gene="lgt"
                     /locus_tag="ACKB38_001108"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGL7979888.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(19108..20043)
                     /gene="hprK"
                     /locus_tag="ACKB38_001109"
     CDS             complement(19108..20043)
                     /gene="hprK"
                     /locus_tag="ACKB38_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGL7979889.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(20173..20349)
                     /gene="rpsU"
                     /locus_tag="ACKB38_001110"
     CDS             complement(20173..20349)
                     /gene="rpsU"
                     /locus_tag="ACKB38_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGL7979890.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(20495..21196)
                     /locus_tag="ACKB38_001111"
     CDS             complement(20495..21196)
                     /locus_tag="ACKB38_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGL7979891.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLL"
     gene            21347..22375
                     /gene="queA"
                     /locus_tag="ACKB38_001112"
     CDS             21347..22375
                     /gene="queA"
                     /locus_tag="ACKB38_001112"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGL7979892.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(23036..24312)
                     /locus_tag="ACKB38_001113"
                     /pseudo
     CDS             complement(23036..24312)
                     /locus_tag="ACKB38_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354309.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT         7284 a         5689 c         4489 g         7094 t
ORIGIN      
        1 ttaaaactgg ttccagatga tgtgcaaatg caagaactgt ttgagtagat gtccacaggg
       61 gttccagact ggacaatctc gccatcattc ataacgaaaa tccagtcact catggcaaga
      121 gcttcttcct gatcgtgagt gacaaagaca aaggtaatgc ccaatcgttg ttgtaattca
      181 cgcaattcgt actgcatgtc tgttctcaat ttcaagtcca gcgctgataa aggctcgtcc
      241 aacaagacca cacggggttg gttgatgata gcacgggcga tggccacacg ctgacgttgt
      301 cctccagaaa gtttgcggat ggaacgtttt tcataacctt ccaactgaac catcttgaga
      361 acttccgcta cacgctgctc gatttctttc ttatcaattt tacgcaagcg aagtggaaag
      421 gcaacatttt caaacacatt catatgtggg aacaaggcat aggattggaa gacggtatgt
      481 acgtcgcgct tgttggttgg aatatcattg atacgaacac cgtctagcat gatatctcct
      541 gtcgtcgcat ccagtaaacc tgcaataatg tttaggatag ttgatttccc cgaaccagat
      601 gcacctagaa gggtgtagaa tttcccttct tccaactcaa agttgatgtc tttgagaacc
      661 ttggtgttgc tgtcttcaaa aactttagag acgtttttga attcgataat tggctttttc
      721 aattggcata aattccttct ttttcataga ttaaccgatc ggggctctgt caggtcccca
      781 ctacctcttg cagggagtaa aaccacctgc atacatcttc gctaccgata ggctttcacc
      841 caagatccgg acttctcttt caagcgtaat acctgagtgt tccttgactt tttcgataac
      901 cgattggatc aagtcctcgt agtctttggc cgttccatct gcgacattga tcataaatcc
      961 tgcatgcttt tctgacactt ctacgccacc gatacgatag cctttcaagc cagcttctga
     1021 aattaactga cctgcaaaat gcccgactgg acgcttaaag accgagccac aagatgggta
     1081 ttccaaaggt tgcttgagtt cacgtaggtg cgtcaagcgg tccatttcct gcttgataac
     1141 ctgatgggtt cctggagcta gggcaaattt aactgacaag acaactgcac cagactcctg
     1201 aatagctgaa tgacggtaac caaaagccaa gtctttagca gacagggttt cgatttctcc
     1261 atccttggtc aagaccttac aagactgcaa gatgtgagca atctcgccac cataggcacc
     1321 cgcattcata aagacagcac cgccaacgct tcctggaata ccacaagcaa actcaaagcc
     1381 agttaaacta tgacggaggg caatgcgagt tgtttcaatc aagttagccc cagcttctgc
     1441 ttcaatggta tagccatcaa cagaaacgtt attgagcttg tcacacaaga tgacaaatcc
     1501 acgaatccca ccatcacgaa cgatgatatt gcttgcattg ccaagaacca tccaagggat
     1561 attttcttgg ttggcaaatt tcacaacgcg agccaactca aaacgatttc gtggaaagac
     1621 caaataatca gcctctccac ctacttttgt ataactatag ctatgcaagg gttccttaaa
     1681 acggatatca attccttcta agatttcaag cattttttct cttacagaca tgtcactctt
     1741 ccttttacaa aattcattcc attataccat ttttagagac atttgacgac cataaaaata
     1801 ccttgtttgg attttgcata agaaaaagag gttcccccct ttttatgatt ttttacaaaa
     1861 gatttccttg gttccatagg cgaccagaac gagctccagt gctagaatca cttcaaccaa
     1921 gactggattt gtcaaccagc ctacttggaa aagagatggt gccagatcaa agaaggcatg
     1981 caagccatag gctgctagga gataaatcca tttcttctgg cgaacagctt ggtaaaccca
     2041 aactgtcaaa agtaattgga aaccaagcgc caagattcgc tcaaaaccaa gcaaataaat
     2101 ctgccagacc gaaagtgact gaatggtttt taacatattt tcagacagta attgcataac
     2161 ctgtggattc tgagtttgaa ctgccgaaag aacaatgtaa agattgagta aactagtaag
     2221 gcctagaaaa atcaactcca agccaccatg ccccagtcca taggccaagg cgtctgcctt
     2281 ttccaaactt ctctttttct ccaaccattt gaagaaaaca agacgagccg tttcctcaaa
     2341 aaatgctgcc atggctaggc catagataat atagataagc ggatgatctt gcaagagggc
     2401 aatactaccg tctttttgag ggtgcaaaat caagatatgc accagttttt ccaaaatctg
     2461 cgaagagaca aagaaagcga ctgcacccaa gcccaagaca gctagattaa tctggtattt
     2521 ctttttggca aaccaaatgc ttcctatcag aaaagctagc aacagcaaca tggtaataat
     2581 gatatgaatg gtcattttct tctcctattc tgccttttca atatcttttt tcatctcgtc
     2641 aacattgaac ttagcaaaca agtattgacg gtcttggact gggaaacgtt ggtccaactg
     2701 gtcaactgct cccacctcga taatgccttc cttgatgaca aaatccatga catgtccacc
     2761 gaaggtcaaa tcatctgata tgaagtgcag atggtagcct gccacactga ccccatggaa
     2821 aatctcaggc gtccagaaac caacaatagt ccccgcaaca ttgtcacgac tatattccgg
     2881 ttgatgggtt gcgacatcag caaacttggt atcgggtgtt gacttaggaa tcatacgcac
     2941 atgcatatgt aaaaattccc cccgaatctt gatagagcgg aaaagatttt ccccatcata
     3001 ataagactca attcgttctt tcaattcctt gtctgtcatc tcaaagcgct ggcggaaaat
     3061 gacctctgcc cgatgcggta ccactgcagc gtaaggaata agggcatctg gtgacacttc
     3121 cacaatttct ggctggtctc ctgacccttt ggcctgataa gccttgccat ccaagacaat
     3181 caattcccca tcaatggaat ccaaggttcc caaaccaagg tcaccatgtt ctagcaattc
     3241 tcctactgtc atagtaccac cataaaggcc agccatcaag gctccaagag tattgtattg
     3301 aaataatttc actggttcct gcacgttctt atccattcac tttcttgtct gttattctat
     3361 aaatcttact cctaagtata ccacatttgc ccctagatgt gaacgagaga aacgctctag
     3421 acattgccaa gaaggaaaaa aaagggtaca atgtaacaaa atcaagggag gtctggaatg
     3481 aagaaacaaa gcaagtacaa agaggtcgtt tcctatctga aaaatggtat cgagtctgga
     3541 cgatttccga cgggtagtcg cctgccttct atccgtcaac tgagccttga ctttcactgc
     3601 agcaaggaca ccattcaacg agccctgctg gaattacggc acgaacaata cctctatgcc
     3661 aagcctcaga gtggctacta tgtattagaa caagggcaac atcaagacct agaaatcgag
     3721 gttaccgacg aacatgccag tgcctatgac gatttccgac tctgtgtcaa tgaaaccttg
     3781 attggccgag aaaactacct cttcaactac tatgacaatc aagaaggatt agaagaccta
     3841 agacagtcca ttcacaaact cctctttgag caagctctct actgcaaggc taaccaacta
     3901 gtactgactt ctggaaccca acaagccttg tttatcctct ctcaaatatc ctttcctaga
     3961 caagccaagg aaatcttggt ggaacagcca acctaccatc ggatgaatcg cctcttgatt
     4021 gcacaggggc tggactatca aacgattgaa cgaggcattg atgggattga cttggaggag
     4081 ctggaaggcc acttcaaaac aggaaaaatt aagtttttct acaccattcc ccgatttcac
     4141 tatcccctgg gacattccta ttctgagcaa gacaaacgat ctattcttaa cttagctgcc
     4201 aagtatgatg tctatatcgt agaggacgat tatctgggtg atttggactc caagaagggc
     4261 caaaccttcc actatcttga tacagaggag cgtgtcattt atatcaagtc cttctcgacc
     4321 agcctttttc ctgcccttcg tattacagca ctcattcttc caaatgctat caaagaagca
     4381 tttgtggcct acaaaaatat cctagactac gacagcaacc tcattatgca aaaggccctg
     4441 tcactctata ttgacagtca attgtttgaa aaaaatcgtt tggctcgctt gaccaatcat
     4501 gaatcttacc aaaaacaaat cgaggaaagg ataactaaaa caccttgtcc ccttcctcat
     4561 tattccctac acgatggttt attgctagac ctgagacagt atcctaaaat cgccagtctc
     4621 aaacacagtc aactgggctt ggacttcttt gaagaggcct atttaagcac ctgtccttat
     4681 caatttgcca aggtgtccct agacaatctg gaaaatgttt taaactattt aaaagcagaa
     4741 ttggaatgac atccaactct gctttttctt attcttcaac ttcagttaga cttgctgctt
     4801 tttcaagata gctttggtaa gttccttcat ccttaagttt ttggacgacc ttgtctacca
     4861 cttctttcaa atcatcacta ttttttctaa gagcaacggc attggcgtcg ccgtccttca
     4921 tcttcaagct gacagttgcg acagctaagc cagcgttttt agcagcataa ctaagtgcaa
     4981 caggctcatc catatgaaca gcatctattt ttccagcctg caattcattg actgcttcac
     5041 ccatattagt tagggaagtt aattgaactt ttggcaattg ttccttgacc attgattctg
     5101 gaacagtccc tttttgggct gcaatattag cactttctag gctagttaaa tccttgtatt
     5161 tttccacatc agccttacga accaagaaac taatcttgtt ttcatagtat gggattgaaa
     5221 aatcaaagac ttcttttctc tcgtcagtag cactaattcc tgcaactgct aggtcagcct
     5281 taccagtttg aagactggtc aaaacattgt caaaactcat gcttgagatt tccaacttaa
     5341 ccccaagttc atcagcgata gcctgagcca tgtcgatgtc tgcaccgact acctggttct
     5401 ttccatcaac caatgattga aattcaaagg gtgcatagtc aggactggtt gccacaacta
     5461 atttcccttt ttgtttgata gcatccacag cagactgaga accattagaa cctgactgac
     5521 aagctactaa gaagaaactt gcaagaaaat tacataaaac aaatatccat ttttttgatt
     5581 tcataaataa acaacctctc tgttaatttt gtataattat aacgatatcc aagttacttg
     5641 tcaagtgttt tttaaatttt tatctcaaaa atattttttc gttcaaaaaa aggagccatc
     5701 agttgatttc aagctccctt ttatacagaa ttaaactatt ttatagttcg acaatcttac
     5761 ctgtttcaaa gtagacaacc cattcacaga tatttttagc atagtcaccg atacgctcca
     5821 agtaggaaat aacttggaaa taatcacgac ccgtaacaat ggcttctgga tttttcttaa
     5881 tctcttcagt cgcaaggtca cggatagttt caaaatagtg gttaatttgc tcatccatgg
     5941 aggccacccg gtatgcgtcg tcaacagaac cattaagata aagatcaagt gctgcttcca
     6001 caacgctttt aacttcacgt cccatttttt taatttcttc ctctacagct ggaatgcgct
     6061 cttccccctt catacggatg gttgcctggg caatggctac agcgtgatcc cccatacgct
     6121 ccacatctga tacagcctta aggacagtca agactgtacg caaatcttga gagactggtt
     6181 gttggagtgc gatcatttca aatgatttct tttccagttt cacttcgtat tcatttactt
     6241 ctgcatcatc ttcgatgacc tcttttgcca ggtcacggtc atgcgtgaca aaagcacgta
     6301 ccgtacgatt gatttgtgag agcacttctt gtcccatagc gtagaactgg ttatgtaatt
     6361 tctctaaatc ttcttcaaat tgagatcgta acatctttca tctccttatc caaattttcc
     6421 tgtaatatag tcttccgttt ccttgtgttg gggatcaagg aacatctgct tggtatcatt
     6481 aaattcaatc aaatctccat ctaggaaaaa tcctgtctta tcagagatac gtgaagcttg
     6541 ctgcatggaa cgggttacca gaagcatggt gtacttgtct tttagaccat acaaggtttc
     6601 ctcaatttta ccagctgaaa tcggatccaa agccgaagtt ggctcatcca agaggatgat
     6661 tttaggacta gttgccaaga cacgggccac gcagacacgc tgctgttgac cacctgacaa
     6721 tccaatagct gaatcatata gacgatcctt gacctcatcc cagatagagg caccttgcaa
     6781 ggctttttct acggcttcat ccagaacctg cttatcctta attccattga tacgaagccc
     6841 gtagacaaca ttctcataga tagtcatagg gaaaggatta ggttgttgga aaaccattcc
     6901 gatttcctta cgtaattcaa ccgtatctgt acgcggactg tagatgttgt gaccattgta
     6961 caccacggat ccagttgtgg tcacctctgg attgagatct cccatgcggt tgagagactt
     7021 gaggagggtt gacttccctg atccagatgg accaatcaag gctgtaattt ccttaggttg
     7081 gaaagatagg gaaacactat tcaaagcctt ctttttatta taataaacgg acaggtctga
     7141 tacctgtaaa atcgcatctg tcatacggtt tcctttctaa ccaaagtgac cagatacata
     7201 gtcattggtg gactgtagct tggcattttg gaaaatagtt gcagtcttgt catactcaat
     7261 caaatcaccc aagtaaaaga agcctgtata gtcacttgca cgagcagcct gctgcatatt
     7321 atgcgttaca atgatgatgg taaagttttt cttgagctca aacatggtct cttctagttg
     7381 catggtcgca atcggatcca aggctgaggc tggctcatcc attaagagga tatctggctt
     7441 aacagagatg gcacgagcga tacagagacg ttgttgctga ccacctgata aggtcaaggc
     7501 tgacttgtgg agatcgtctt taacctgatc ccagagggca gcctgacgaa gggaggtttc
     7561 tacgatttca tctaggactt gcttatcctt aactccagca cgttcatgcg caaaggtaat
     7621 attacggtaa attgacttag caaatggatt gggacgttga aaaaccattc caatgtgttt
     7681 acgcatttca taaacgttga tttctggacg gttgacatca attccacgat agagaatctg
     7741 cccagttact ttagcaatat caatagtatc attcatgcga ttgagactgc gtaagtaggt
     7801 agatttcccc gatcccgacg ggccaatcaa agctgtaatt ttatttcttt caaattgcat
     7861 atcaatcccc ttaatggatt catttttacc atagtaaaca tggacatcct tagtagaaag
     7921 ggctactttt tcttcaggaa aggtaaggat atgcttctca tcccagttat atgttgacat
     7981 ggcttctcct ttaggcagcg gttaatttct tgtgtagata gcttccgaac ttacgagctc
     8041 caaagttaaa aatcaggata aagatcagga gcacagcggc agaacctgct gatacaatgg
     8101 ttccatctgg aatagtgcct tcactattga ctttccagat atggacagcc aaggtttctg
     8161 cttgacggaa gatagagatg gggctagtca cactgaggat attccagtta gaccagtcaa
     8221 gagctggcgc cgattgccct gctgtataga tcagagctgc agcttcgcca aagatacgac
     8281 cagatgccaa gacgacaccc gttacaatac ctggaagcgc ttccggaata acaacatgaa
     8341 ccactgtctc ccagcgagaa atcccaagag ccagaccagc ctcacgttgg gtatggtgaa
     8401 cgtgtttcaa actatcctct acattacgcg tcatctgagg caagttaaag actgtcaagg
     8461 ccaaggcacc tgaaatgatt gaaaatccat actcaaactg gactacaaag atcaagtaac
     8521 caaagagacc caccaccact gatggtaaag aggacaaaat ttcaatacaa gtccgcacaa
     8581 agttggtaac aggacctttt ttagcatatt cagccaagta aatcccagct cccatagaaa
     8641 gaggtacaga aataatcaag gtaatgacca ataggaaaaa ggaattgtaa agctgaatgc
     8701 caatcccacc acctgcttga aaagcagaag accttccagt caagaaagac caagagatat
     8761 ggggcaagcc ccgaaccaag atatagagaa tcaaggaagc caagattgtc acaatgatgc
     8821 tagcaatcgt atagaggaca gctgttgcaa gtttatctaa tttcttagcg cgcataattt
     8881 ttctttcctc tttctttcgt aatcaattta atcacactgt taaaaactaa gctcatcaag
     8941 agcagtacca aggccagtga ccagagaaca ttattatgta cagttcccat gacagtgttc
     9001 ccaattccca tagttaatat agaagttaaa gttgcagctg gtgtggtcaa ggaagttggg
     9061 ataacagctg agtttccgac aaccatctgg atagctagag cctcaccaaa ggcacgcgcc
     9121 atcccaaaga ccactgcagt gaaaatacca gaacgggccg ccttcaagat cacacgccag
     9181 atagtctgcc agcgagtggc tcccatagcg aaactggctt cacgataata acgaggaacc
     9241 gcacgcaagc tatccgttgt cataaaggtt acggtcggca aaatcatgac aaagaggacg
     9301 gaaatccctg acaaaatccc aaaaccagtc ccaccaaaga cactgcgaac aaagggaacg
     9361 acgacttgca agccaataaa tccgtacact actgaaggaa tcccaaccag gagttcaata
     9421 gctggttgca aaatcttcgc cccttttggt gatacttcgg tcataaaaac tgctgcacca
     9481 atagcaaagg gtgttgcgat aagggctgag agaatggtaa cgataaagga acccaaaatc
     9541 ataggaaggg caccaaattc tttactagaa ggattccaag ttcctcccaa aagaaagtca
     9601 aagatattca caccattgac aaagaaggtc gacaagcctt tttgcgctac gaaaaccaaa
     9661 atcatggcca caaggatgac tatcaaagaa agacaggcaa aggtcaaacc ttttcctaat
     9721 ttctccagac gagaattctt tgatggaagc aacattttct tagctaattc ttcttgattc
     9781 attattgtct cccttccaac actgtcacag ttccggcagc atctttttca accttcattt
     9841 ccttaatcgg aatatacttc aatcctttga caatcccttc ttgggtctca tccgagagaa
     9901 caaaattgag aaattctgca gccaactcat tgggctgccc caatgtatac atatgctcat
     9961 aagaccacaa gggccaatta ttgctactta tattttctgg acttaagtca tagccattca
    10021 acttcatgct tttgaccgaa tcatctatat aggtaagaga taaataagag atagctcctg
    10081 gactttttga tacgattgat tttaccgctc catttgaatc ctgctcctga ctttgcatgg
    10141 cagactgacc ttccataatg acagtatcaa aggtagcacg agagccagag ccggctgccc
    10201 gattgataac agagatgggt aagtccttac caccaacctc tttccaattg gttacctcac
    10261 ctatgaagat ttgacgaagt tgctctgtcg ttaggttatc aacatcaacc tccttattga
    10321 caatcagagc caagccagct accgcgacct tgtggtcaac aagagcagaa gcatcaattc
    10381 cgtctttttc ctcagcaaat acatctgagt ttcctatatc aactgcccca gactgaacct
    10441 gggacaagcc tgtaccagaa cctccccctt ggacattgac cgtttttcca acatggatcg
    10501 tgccaaattc atctgccgct acttcaacca agggttgcaa ggcagttgag ccaacagccg
    10561 ttatggattc tccacgatca atccagctag cacagcctac taaacaagcc gtcagccaaa
    10621 aagcgataag agacagagca agcttttttc tttttttcac tgtttttctc ctcgaaaata
    10681 attatgaata ctgtgaattt tttaagtagt tctttatgag ttgacgcatg aattcttacc
    10741 aaatttctgc gcaattgatt atttatataa tataggctat attactcttt cctaacctcc
    10801 ttttttcata tgtggataaa atctcttgtc tatcccttcc cccattgtca cccattatag
    10861 tcatttcgtg tctctttttc ccctttttaa tgcaagggaa attactctcc ttagatgata
    10921 atccaaaagc tagaaaggta tctcaaacct ctctactctc ccagactagt ttacaactaa
    10981 aaggaaaaga ttctatttta tgagaaatct agtttacaag cggtaagaac gctaataact
    11041 aaacttcttg tactctttga aaatctcttc aaaccagtgt tttgagctat ctatggctag
    11101 cttcctagtt tgctctttga ttttcattga gtagtaaaac tacatgtaat ggcaatcaag
    11161 atatcaagaa tcatcctact aaaaaaatcc atactttcac tataacatag aataagatat
    11221 ttgactagca ttttcatttg aatctgaggc cttttggaaa ataatttttc aaaacatttc
    11281 cagtaacctt tgcaaagccc aagccattgc ctttaaccaa aacttggtac caaccatttg
    11341 gcagactttc tgccagctga acggtttctc cagccgcata cttgacaaac gcttcttggc
    11401 caatttcaac cgactgttcg acctgactcg gtttcaaggc taaaccaaga gcgaaactgg
    11461 gctcaaagcg tttcttctta aaagtaccca gatgcagtcc attgcgagca atcttgagct
    11521 tccataaatc tggcaaaagt tctggcaaga gataaagctg gtctccaaaa atctgcaaga
    11581 tacccggtag attgaccttc aaatggtttt gggcaaattc ctgccacaag gcaacttgtt
    11641 cacggctgag gttactctta cttgccttaa atttaggagc tggattgtta cccttaaact
    11701 gtagatgggc aacaaactga ccctctccct taaactgatg aggatacatc cgagccgttt
    11761 ctggcaggtc aataccagct accattccat tgatatgctc tactggcaac aagtcaaaat
    11821 catactcttc cagcaaccaa ttgacaatct cttcgttttc ctcgggtgcc caggtacagg
    11881 tcgaataaac cagatgacca ccttcagcta acatggtcac tgcatcctcc agaatttctc
    11941 tttgcaagct agcacattga ctcggataat ctaagctcca atagtccata gcatcaggtt
    12001 gcttacgaaa cattccttca ccagagcaag gggcatcaag aacgattaag tcaaaatagc
    12061 ctttaaagac cttgaccaag cggtcggcag attcattggt caccacgaca tttgtcgctc
    12121 caaaacgctc catgttttca accaaaatct tagcccgttt gcttgaaatt tcattggaaa
    12181 caagtagccc ctcccctgct agataggctg ccagttgagt tgatttgccc cccggtgcag
    12241 cagccaagtc caagaccttc ataccaggac tgggttgggc tacttgagcc accatttgag
    12301 cagcaggttc ttgcgaataa actaaacctg tagcatgctc aggcgatttc cctgaaacct
    12361 tcccatagtg gccccaaggg gtttgagtaa tggcatcaga aaaggaaagt tgctcttctt
    12421 ttaagggatt gacccgaaag gccgaaaccg cttcctcctc aaaagaggca agaaaatctc
    12481 ttgcctcatc tcctagtatc tctttatatt tttcaacaaa tccttctgga aattgcattt
    12541 aagttctttt cctttcgtaa atataggact gaatttcctc ctgcatctca agaggcacca
    12601 tcatgaccgg ctgtctggtt tgaaaatcag gagcttcacc aaaaagggtc acaacccgat
    12661 agcccagact ttcccctaaa atactagctg cggcataatc ccatggttgc agataagtga
    12721 gataggtcaa caaacgccct gacaaaatct tggcaaaact aatggccgca cttccataga
    12781 cacgaacacc aagaaccgct cggctcaaat cagccagccc ccattcattg gtttccagca
    12841 taccactatt ccctgcaatg agaaaatctc caagtggttt agttttaaaa ggagctaggg
    12901 acctatcatt tagacaaact ggaaattccc caccaccgtg gtaacaatcc cctttgacca
    12961 catcataaat cagaccaaac tgtccctgac cattttcaaa ataagccatc ataacagcaa
    13021 aatcttcctg ctgggctaca aaattattgg taccatcaat gggatcaatg acccaaacct
    13081 tgccctcttg aaccgaggct cgcagacaac cttcttcagc acaaatctta tcctcaggat
    13141 aacgggacaa aatctcacca accaagagtt cctgaacttc tttgtccagt ctggtcacca
    13201 aatctgttgg agaggacttg gtttcaacac gcaagtcttc ctgcatatgg tcaagaatgt
    13261 actgacctgc tttcttaaca agctctttag caaattcaaa tttactttcc aagagaaatc
    13321 tttccttccc ctttttcttt ggcagcctga actgctcggt aaagtgaata gccactaacc
    13381 gtttcaaatt ctcgtcccaa acgtttctct tcgctcttgc ttggcacgac cgctttgaat
    13441 cccttatagg agtccagtaa ctttttagcc tctactttgc cttcataggc agcttcaaca
    13501 tcattaaaaa aagaaagcac tgaagcaagt tcttcagtgc tccacgacaa atctagtggg
    13561 taactatact gtttgttcat taactaatac cagctctcat tcttgcttct tttagttctt
    13621 gcttacgata actacgaggg agaaaagcac gaatctcatc ttcattaaaa ccgatttgca
    13681 tacgcttggc atcaataata attggacgac gcaaaagact aggatactgc tcaatcaaat
    13741 gaagcaattc cgataccgaa atactctcta catcaatatt caatttttga aaaatttttg
    13801 aacgagttga aatgatgtca tcagtaccat tttcggtcaa ggaaaggatg tgttgcaatt
    13861 cttttcttgt taaaggactg gtcataatat tgtgttccac aaagggaacc ttatgttttt
    13921 ctaaccaggc cttagcctta cgacatgatg tacagctcgg tgatagaaat agtgtaatca
    13981 tgcttttctc ttcttatcta tactttgcta cttctattat acaaaaaaat aaagcgcttg
    14041 actagggatt tttagaaaaa aagcctattt tttcaagaaa aataggcttt ttgcgaacga
    14101 ttgacacaat tggatttggt taattcactc ttaacgatgg ttttaaacga tatatatttt
    14161 tatatatgta aattaaaaac atctttcctt tcacttccta cgacttttca gatacagata
    14221 gccaaagaag ttttcataga gggcaaaaaa gaggaggaag gcatgaagaa agaaggtctc
    14281 tggcaaaatc ataataacag gatccttggc tggatcaaaa agccaggtat catctcccac
    14341 aaagagaatt tgatggaaaa gagtaaagaa ttggtcaaaa ccaatcaaaa ctcccccaag
    14401 tccaatcatc acaggtaaga ctactagagc caggagactt tttcgataaa gagacaaaaa
    14461 gtcctttttc acaatcctat tgacaaagac atagaaactt ggcagtgtca ctagagctac
    14521 tagctgaacc aaatgaaaga gattcttgac cactgcgaaa tggtgcagac cagctgctga
    14581 cgaacgaaaa tcaggcatct gtaagacctg actaaaagga ttggtcagat aattcatcaa
    14641 gatatgaaaa ttgtattgaa tggtttctgg ttttagatag actcgattcg ttaagtttag
    14701 ccactgaatc tccataggat agaaaatcca agccagataa atggtcagaa ggatggagag
    14761 ggagaggaga aagagcatag agccccaaaa gatcaattta gttttcatca aaatcccact
    14821 ccgcaaggct agaaaccaca tgtgtcggtg cgattggcag gccagctact tcttctgcct
    14881 tagtaaaacc tgtcgtcacc aagagcgttg gaatgccatt gtcaatccca gcccgaatat
    14941 cagtcaaata attgtcccca accatgatta actcttcacg ttccaaacct aagtgctcaa
    15001 ccgccttgtc cataatgatg gcatttggtt ttccgatata aaccggcttc actcgtgtcg
    15061 ctacttcaag cagcgtaatc agtgagccag cacctggcaa aagaccgcgt tccgtcggga
    15121 tgttgaggtc aggattggtt ccgataaaat gggcaccctt ttgaatagca agagttgctg
    15181 tggcaaattt ttcatagtcg acttgccaat ccagaccaac taccacgtag gcaggttttt
    15241 ccttgtcttc cacataacca gccgccttga tggcttcctt gagtcctgct tctccgacga
    15301 catagacggt cttttcaagc cccaaatcat tcatatagtc gatggttgcc aaagtcgctg
    15361 tgtagacagt cgataggggc gtatcgatat taaaattctg agccaacatc tccttaacac
    15421 tctctggagt gcgggttgta ttgttggtta caaagagata gggaatgtcc cgcttttgca
    15481 attcatgaac aaaagtctct ccagcaggga ttcggtcttt ccccttataa atggttccgt
    15541 ctaaatcaat taaatagcct ttatatttca tctatttctc cctaagcctt ttttatttct
    15601 tgccaagtaa tgattgcttg ggcattgata accccatcac ttgtaatttc atgcttgctt
    15661 tccagtccag tccgttcaac agccgatgta atcaccccac ctggtcgaac ttccttgaca
    15721 tacttgaggt tgattttctt gggaatatag tgggtcaaaa aatccgctcc catgacctca
    15781 aaaatccagt ccaagtattt actgttattg acatgaccat tcatatccaa gtcgtaaaaa
    15841 cgaacatggt aatccttgct gatcggttct tccaaggact catacttcgg tccacggata
    15901 agttttttat caaaatcaga ctggtaagga gccacaatct caggttcaac aacatggact
    15961 tttcgactgt cgcggtccat gagaacaaag gtcgccatca tgtggatgag ctcctgctcc
    16021 gcttcattat aaatagtaaa gcgacggtag caaaaaagtc gattgtagct caaggcttcc
    16081 gtttcgatgg taatttcttc cgcaaaacga ggcaaacgaa ccacctcaat atcatattct
    16141 acgataatcc agaccagatt atattcttcc aaaatggcct tatcactaac tcccagttca
    16201 atcgactgca tccctgaaac ttgcagtgac agcaaaatca catctggaag tttgatatga
    16261 ccgttcatat cagccatatc aaaaggaatt ttcattttca tttgataagt taagcccatg
    16321 atcctactcc aaaataaatc gttctgctac agtatctccc aaaaagagac ctctctttgt
    16381 catgcgaacg tggtcaccct caatctgcat gaggccttgt tgaaccaaat ctctgacaat
    16441 ttctccataa agtccagcaa aagactgtcc aaatttttcc tcaaatcgcg ccatggaaac
    16501 cccggatttc ttgcggagtc ccaagaacat ttcttcttcc atttgctcct tttgactcag
    16561 gtgatcttct gtaatacaag cattgccttc ctcaaccgca ctgagataat gacgaatggg
    16621 accatgattt ttatagcgta ctccattgac ataaccagat gcccctgcac caataccata
    16681 gtattcagca ttgtcccagt acatgagatt atgacgactt tcaaaaccgg gtttggagaa
    16741 attagaaatc tcataatgct caaaacccgc tcgctccagc tctgcaatga tgtactcaaa
    16801 catctccgct tctagttcct ccttaggcag aggcaatttc ccacgtcgca tccggttcat
    16861 aaagaccgta tggttttcta aaatcaaact atacaaactc atgtggggaa tatccaatcc
    16921 aatggcttta gccacatttt cctttacttg ctccatggtc tgaccaggca gagcataaat
    16981 caaatcaatg gagatattgt caaaaccagc cagtttcagg cgatcgatat tttcataaat
    17041 atccttctcc aaatgactgc gcccaatctt tttcaacatc ttatcatcaa aggtctggac
    17101 acctagcgaa acacgattga cagccgaatt tttcaaaaca gctatcttat ccgcatccaa
    17161 atcgcctgga ttggcttcaa tggtcaactc ttccaagaca gacaaatcca agtttttagt
    17221 caagccattc agtaacacct ccagttgcgg agccgacagg gctgtcggtg ttccaccacc
    17281 gatataaagg gttgacaact tttcaatatc ataagaacga aactcttcca gcagatgctc
    17341 taaatagctg tcgactggct gatttttgat gaagaccttt gaaaaatcac aataataaca
    17401 aatctgggta caaaatggga tgtgcacata ggctgacgtt ggttttttct gcatagtaat
    17461 tattatacca caaagactag attccagata aaaatcacca tccccagata catagtccgt
    17521 ccggagatgg tgatggttta ttcttctgtt atatcaatca caatctcttc tgagtcatca
    17581 agagcttcgg ctttttcttg ccattgctcc ttgagattat ttaattgatt ttttgatgct
    17641 tctgtcgctt gaaaagcata ggatttagtt tgagcaagta tactgtccac agtgatttca
    17701 cctgactcaa cctgttcttt tgttttcaga acaaaatctg tagcctgctc cttaacttct
    17761 gtcagttttt cacagacttg ctccttggca tactccggat cttctctcaa atcatctaga
    17821 aaatcttgag cctgactgca aacttgtttg cccttatcac ttgttaaaaa caaggcaaga
    17881 gctgcacctg aaacggttcc taaaaggatt gaggataatt tacccataag gattctcctt
    17941 ttttattttt tgaaaaattt acttgcaaga cgaagagctg acagacttgc accagtcttg
    18001 agtgtttttg aaccagctga tgaagctttc ttgctcaaga cacgcgcatg gtcattgagg
    18061 tctgaaacag atagagataa atctgcaaca gcactgaaga gtggatcaat cgtagccacc
    18121 ttgacattga tatcatctgc caagacattg accttagcca acaactcatt ggtgtgatgc
    18181 aaggtcacat ccacatctga agtcaaggtt ttaatcgtct tttctgtttc atcgatgaca
    18241 cgaccaagct tttgtacagt aatgatcaga tagaccaaaa agacaatcaa agctagggca
    18301 acaagaatat atgcaacttc taacatttag ttttcctcct ctgtaatata gtaaggggcc
    18361 ttctttcgat tttgataaat aacgatcatt ataccgagac cgataaggac aactgacagc
    18421 cattgggaca ctcgaaagcc gaagaacatg agactatctg ttcgcatacc ttcgataacc
    18481 atacgaccga aaccatacca aatcaagtaa aaggccgtga tatgacctcg tctgagactc
    18541 ttccatttcc gtctaaaaat cagaatcaag gcaaagccaa gcagattcca tagagactca
    18601 taaaggaaag tcggttgacg gtagctcccc tcaatataca tctggtcacg gataaagcca
    18661 ggtagataat ccagattatc cactgttgca ccataagctt cttggttaaa gaaattaccc
    18721 caacgcccca aactttgagc aatcataacg ctaggcgccg caatatctag aaaatcccaa
    18781 gtattgatga gtttacggtc agcaaagata tagagcacaa gagccccagt tatcaaacca
    18841 ccgtaaatgg ccaaaccacc attccaaatg gcaaaaatct ctcctaaatt ctgactatag
    18901 taatcaaatc ggaaaataac atagtagaga cgagctccta aaatagccaa gggaaaggct
    18961 actaagataa aatctaaaat atcgtctggt atgatcttct ttctaggtgc ttctttcatg
    19021 gtcaaataaa ccgcaagaat caagcctgtc acaatacata aggcatacca acgaatggct
    19081 aggggtccta gttgaatagc aattggatca agcattttgc acctcatttc gagcgattag
    19141 acttgtcagt cgttcgtcga acaaacgggt cgcatcaaag cccatttcct tggcacgata
    19201 attcatggca gctgcctcaa tcacaacaga gatattacga cctgttttaa ctggaatacg
    19261 aatacgagga atggctacgc cagaaacttc aagttcctct gcattatttc caagacgatc
    19321 aaaggtctta tgcgtatcgt aattttccaa atagacagca agctgaacct gtgaagaatc
    19381 cttgacagca ctcgcaccgt agagactcat aacatcgata ataccaaccc cacgaatttc
    19441 aatcaagtgt ttcaaaattt cagctggttc accccagaga gtaatctcat ccttggcaaa
    19501 gatatcgaca cggtcatcgg ctaccaaacg gtgaccacgt ttgacaagct caagacctgt
    19561 ctcgctctta ccaattccac tatctccctg aatcaagacg cccatcccat aaatatccat
    19621 caagacaccg tgcacactgg tacgttctgc caaacgagaa tccagatagc tagataactc
    19681 tccagataaa cgactggtag ctgcacggct ggttaaaata gcaatcttac attctctagc
    19741 agcctttaac atctcctctg gaaccaccaa accacgggca acaatgactg ctggtgtctc
    19801 aggtagaaac atttttttca aaacttcata acggctgttg gaaggcatgc tgatcagata
    19861 agaccactcc ttcatcccca aaagttgaat ccgctctggt gtatagtagt caaaatagcc
    19921 tgtcatttca agaccaggtc gcgtaatatc cgctgtattg atttcctttt caagcaattc
    19981 tggttcacca tagacaatgt ctagtctaag cttttcaatc acttctttta ctaaaaccga
    20041 catcatttcc tccttcaaat gagttcttct ctctattata tcaaattgta ggaggaagtt
    20101 tcagattttt gcaggctttg gagtatttat ttaaaagaaa aaggactaga tttctctagc
    20161 ctttcatttc aattagaatt ttttacgttt acgagctact tctgatttac gtttacgttt
    20221 tacagaaggt ttttcataga attcacgttt gcgtgtttct tgaagagtac cagctttagt
    20281 aaccgcacgt ttgaaacggc gaagtgcgtc gtcaagagat tcattcttac gtactactgt
    20341 tttagacatt tttttcactc ccttcaagtt caagatttat tctattttac acgtaaaagg
    20401 aataattgtc aagcaaaaat gcggtttaat tttgattttt tcttccattt atacaagagt
    20461 tgaaagcgtt caatagaaca ttgctttttt atttttagag taagctaagc gcttcagcat
    20521 ctgcgatgat ggttacatca gggtgatttt ggaggctact tgcaggtagg ttctcagtca
    20581 ctgggccaga tactgttccg gcaatggctt ctgctttcga ctcaccgtaa gcaaaaagaa
    20641 taatagactt ggcatccaaa atgtttttaa tccccattga aatagcttgg gttgggacgt
    20701 cttcaatctt ggcaaagaag cgtgcattgg cttcgatagt agactggtca agttctacta
    20761 gatgcgtttg actgtcaaat ggagtgccag gctcattaaa gccgatatgc ccattgcgac
    20821 cgattcccaa gatttggagg tcaactggat ggtcagccaa aatttggttg tagcgttcaa
    20881 cttcagcttc agcattgtcc ttaacaccac gaggcaagaa actttcttta aatggttttt
    20941 ggttgaacaa gttttcttgc atgaagtaac gataagactg tggattgtcc ccatcaaggc
    21001 ccacatactc atcaaggttg acactggtta gatttgaaaa atcaaggtca ctctcaacaa
    21061 tttccttgta aaattcaagt gggctacttc ctgtcgcaag tcctagagtt tgagctccat
    21121 tagccaattt ttccttcaaa atctcaaaag caacttttcc accttgaact tggttttcaa
    21181 ctttaataac tttcatcttt tcatcctcca tttttgtcta tatttattat atggtataga
    21241 ccaatttttg tcaagtgaaa acgatttaat ttctaaaaaa agagcgtcca aacttgaaac
    21301 gtgttataat ggataggatt agaacttaga aagaatgaac aaatatatga atacagctga
    21361 ttttgatttc cacttgccag aggaattgat tgcccaaacg ccccttgaaa aacgtgatgc
    21421 ctccaaactc ctcatcgtca accgtgagac aggagaaatg caagataaac atttccactc
    21481 tattattgat atgctggaac ctggtgatgc ccttgtcatg aacgacaccc gagttctccc
    21541 tgcccgcctc tatggtcaaa aagtggagac aggaggtcat gtggaacttc tcctccttaa
    21601 gaacactagt ggagacgagt gggaagttct ggctaaacct gccaaacgcc tcaaggtcgg
    21661 tactcgtatc agctttggtg atggccgcct cagcgctgtc gttacagaag aattgaccca
    21721 cgggggacgc attgtccgct ttgaatacca aggaattttc ctagaagtct tggaaagtct
    21781 gggagaaatg cctctgccac cttatatcca cgaaaaatta gatgaccgtg aacgttatca
    21841 aaccgtctac gccaaggaaa gtggctctgc tgcagcaccg actgctggtc ttcacttcac
    21901 caaagaactg ctggcagaaa tccaagctaa gggtgttcat ctagtctatc tgactctcca
    21961 tgtcggactc ggaaccttta gacctgtttc tgtggataat ctggacgaac acgaaatgca
    22021 ctcagagttc tatcaacttt ctgaggaagc tgctgccacc cttcgctctg tcaaaaaaaa
    22081 tggtggtcgt gtcatcgctg tcggaaccac ttctatccgc accttggaaa ctattggttc
    22141 caagtttgat gggcaaatcc aagcagattc tggttggacc aatatcttta tcaaacctgg
    22201 gtatgagtgg aaggtcgtgg atgccttctc aaccaacttc cacctgccaa aatcaactct
    22261 ggtcatgttg gtttctgcct ttgcaggccg tgaattagtc ttagatgcct accaccattc
    22321 catccaagaa cactaccgct tcttcagttt tggtgacgcc atgtttattt attgagaaag
    22381 aatttctcta aatcttctaa taccaataaa tcgctaagat attatttcaa agaacatcta
    22441 caattgaaac tctagctagc tgtagaagag gcctagtaca ttgaaattaa aatgcttccc
    22501 cctagcttcg aaaatattgc catagattgc gttgactctc caaattgatt catctatatt
    22561 ttatttcagc ttcctatact ttcttcgctg tttgtaaatc aaaatgcaag acacatgagt
    22621 agcaccatat ttgttactct tatctgtcct ctcaagagac tattatgagt tatttcagaa
    22681 tcattcacta ctttgaccct gactctcctt agtctcaaaa tcaaagactt atactcttca
    22741 aaaatctctt caaaccgcgt caacgtcacc ttggattata tatgtgactg acttcgtcag
    22801 ttctatctac aacctcaaag cagtactttg agcaacctgc gactagtttt ctagtttgct
    22861 ctttgatttt cattgagtat taaacaaaaa gtgaacaaat ctgaattcta atgtacagaa
    22921 gactaggctt gttcactttt ttatagtcgc tataagatga ccttatctat agctttttat
    22981 atataattat atattcagac atactattat caattttgtc gcagggagga atctgttaac
    23041 gcacccattc accattatca ttgactctat agccatctat acttgtattg accgctaact
    23101 cacccgatgt atttacataa taccatttac caccaacttg gaaccattga ttgactttca
    23161 tagaaccgtt gctgttgagg tagtaccatg aactattaac ttgtacccaa cctgttgcca
    23221 tggaaccatc agtattataa aaataccaca taccattttc ttgtttccag tctgttgttg
    23281 gagcaactgc tttagctggt tctactgcta catctgttcc ttggttagat gtaacagata
    23341 caggatacga aggaatagat gattgctcag gaacaacaac tttttcaggt tctctcgtcc
    23401 ctctccttat acgtcttttt accatctctt tagtaatttg acgagaagta gtttcttcaa
    23461 ttgttccatc acgttcatct acagtataga ttgtagtaag agtaatttac caatttctcc
    23521 tacttcttct acttcttgac ttttatcaag agttgggcca tcgagatatt ctgtttcgat
    23581 tggaatttct tggacaagaa cttggggctt ggttcttttt ttaacaactc ttgtttgaga
    23641 gtcttttttt tgacttaaag tactctcagt tacttgtcca ctctttccat ctacattata
    23701 agttatcgtt gtaactgttt tcccattctt tcctagagta atctcttgct cctgtcctgc
    23761 agaaaggtca ttgtctgctt catatttagt agcaaatgga acaagaactt cttcaacctt
    23821 gcttttagct ggaactttga taactgtatc cgtggcttct tttctatcaa cagtaacctg
    23881 ttcggtaaca taaccagtct ctggattaac atcgtaggtc cttgtcgtag ttacatagcc
    23941 atcctctcca tcaattgtaa caggattttc actacggtct tttgtttcat ctttttcata
    24001 acgaattcgc gtacttgaaa ttttcttggt tactacctta ggtttagtcg ctacttttac
    24061 aataatatcc ccattgtcag cgtcatcata ctctattccc tcttctttat ctctagtatc
    24121 atctctgaca tattgaatcc catcagcagc atgaacaaaa cttgtattca gattcctcct
    24181 aaaaataaag ttagcccgat taccgcagaa ccaaaaatct ttccgagttt acgtattgca
    24241 tagcgcttat tagtattaga ttttgccatt acatcctact tctagtatag catcttttct
    24301 atcaaacgtt aaacaatata ctttatatat aaaatagact tagaatgata tattgattat
    24361 tgaactaaca ctttaactat atcgtaatca atctcatata taaaggattg cagacatctt
    24421 atctaaatac atgctaatat atttagatac aaacattcca acttgataat tttcactcat
    24481 ctttcatcat tccttataca actatgcagt ataaatagaa tagttttctc atcagaatga
    24541 gactatttta atatta
//
LOCUS       DBDESG010000027        24354 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.027, whole genome shotgun sequence.
ACCESSION   DBDESG010000027 DBDESG010000000
VERSION     DBDESG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.027"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..289)
                     /locus_tag="ACKB38_001114"
     CDS             complement(<1..289)
                     /locus_tag="ACKB38_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGL7979893.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIV"
     gene            483..980
                     /locus_tag="ACKB38_001115"
     CDS             483..980
                     /locus_tag="ACKB38_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGL7979894.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            980..2635
                     /gene="dnaX"
                     /locus_tag="ACKB38_001116"
     CDS             980..2635
                     /gene="dnaX"
                     /locus_tag="ACKB38_001116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGL7979895.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGANTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2663..2857
                     /locus_tag="ACKB38_001117"
     CDS             2663..2857
                     /locus_tag="ACKB38_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGL7979896.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            3007..3777
                     /gene="sufC"
                     /locus_tag="ACKB38_001118"
     CDS             3007..3777
                     /gene="sufC"
                     /locus_tag="ACKB38_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGL7979897.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3805..5067
                     /gene="sufD"
                     /locus_tag="ACKB38_001119"
     CDS             3805..5067
                     /gene="sufD"
                     /locus_tag="ACKB38_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGL7979898.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIECVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIVGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            5078..6304
                     /locus_tag="ACKB38_001120"
     CDS             5078..6304
                     /locus_tag="ACKB38_001120"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGL7979899.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            6291..6731
                     /gene="sufU"
                     /locus_tag="ACKB38_001121"
     CDS             6291..6731
                     /gene="sufU"
                     /locus_tag="ACKB38_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGL7979900.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6785..8197
                     /gene="sufB"
                     /locus_tag="ACKB38_001122"
     CDS             6785..8197
                     /gene="sufB"
                     /locus_tag="ACKB38_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGL7979901.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVYYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8428..9669)
                     /gene="pbp3"
                     /locus_tag="ACKB38_001123"
     CDS             complement(8428..9669)
                     /gene="pbp3"
                     /locus_tag="ACKB38_001123"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGL7979902.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9772..10881
                     /gene="cozE"
                     /locus_tag="ACKB38_001124"
     CDS             9772..10881
                     /gene="cozE"
                     /locus_tag="ACKB38_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGL7979903.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFFYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSHLYENHKIMKERERELAK"
     gene            11415..11762
                     /gene="ssrA"
                     /locus_tag="ACKB38_001125"
     tmRNA           11415..11762
                     /gene="ssrA"
                     /locus_tag="ACKB38_001125"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11945..12032)
                     /locus_tag="ACKB38_001126"
     tRNA            complement(11945..12032)
                     /locus_tag="ACKB38_001126"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11996..11998),aa:Ser,seq:gga)
     gene            12194..12934
                     /locus_tag="ACKB38_001127"
     CDS             12194..12934
                     /locus_tag="ACKB38_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGL7979904.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPYSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGINGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12931..13842
                     /gene="pfkB"
                     /locus_tag="ACKB38_001128"
     CDS             12931..13842
                     /gene="pfkB"
                     /locus_tag="ACKB38_001128"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGL7979905.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13839..15791
                     /locus_tag="ACKB38_001129"
     CDS             13839..15791
                     /locus_tag="ACKB38_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGL7979906.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15928..18180
                     /locus_tag="ACKB38_001130"
     CDS             15928..18180
                     /locus_tag="ACKB38_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGL7979907.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(18225..18653)
                     /locus_tag="ACKB38_001131"
     CDS             complement(18225..18653)
                     /locus_tag="ACKB38_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGL7979908.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18791..19933
                     /locus_tag="ACKB38_001132"
     CDS             18791..19933
                     /locus_tag="ACKB38_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGL7979909.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19942..21156
                     /gene="thiI"
                     /locus_tag="ACKB38_001133"
     CDS             19942..21156
                     /gene="thiI"
                     /locus_tag="ACKB38_001133"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGL7979910.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21291..22115
                     /locus_tag="ACKB38_001134"
     CDS             21291..22115
                     /locus_tag="ACKB38_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGL7979911.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            22137..22876
                     /locus_tag="ACKB38_001135"
                     /pseudo
     CDS             22137..22876
                     /locus_tag="ACKB38_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22889..24055
                     /locus_tag="ACKB38_001136"
     CDS             22889..24055
                     /locus_tag="ACKB38_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGL7979912.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT         7134 a         4551 c         5396 g         7273 t
ORIGIN      
        1 tgacgataga agtttcagcg accatcattt ttgaacagtg atagcacttg aaacgacgct
       61 ttctaagtag aattctagta ggcataccag ttgtctcaag gtaaggaatc ttagacggtt
      121 tttgaaagtc atatttcttc aattggtttc cgcactcagg gcaagatggg gcgtcgtagt
      181 ccagtttggc gatgatttcc ttgtgtgtat ctttattgat gatgtctaaa atctggatat
      241 tagggtcttt aatgtctagt aattttgtga taaaatgtaa ttgttccata tgaatctttc
      301 taatgagttg tttggtcgct tttcattata ggtcatatgg gacttttttt ctacaataaa
      361 ataggctcca taatatctat aagggattta cccactacaa atattataga gccaaaaatc
      421 ctttgtttac taaacaaggg atttttcttt tgtctctgct ccttttttga tataatagtt
      481 ctatgttaaa atcagaaaaa caatcacgtt atcaaatgtt aaatgaagaa ttgtccttcc
      541 tattggaagg cgaaaccaat gttttggcta atctttccaa cgccagtgct ctcataaaat
      601 cacgttttcc taataccgta tttgcaggct tttatttgtt cgatggaaag gaattggttt
      661 taggcccctt ccaaggaggt gtttcctgca tccgtattgc actaggcaag ggtgtttgtg
      721 gtgaggcagc tcactttcag gaaactgtta ttgttggaga tgtgacgacc tatctcaact
      781 atatttcttg tgatagtcta gctaaaagtg aaattgtggt gccgatgatg aagaatggtc
      841 agttacttgg agttctggat ctggattctt cagagattga ggattacgat gctatggatc
      901 gagattattt ggaacaattt gtcgctattt tgcttgaaaa gacagcatgg gactttacga
      961 tgtttgagga aaaatcttaa tgtatcaagc actttatcga aaatatagaa gtcaaaactt
     1021 ctcccagtta gttggtcaag aagttgtggc taagactctt aaacaagcgg tggagcaaga
     1081 gaaaataagt cacgcttatc ttttttctgg tcctcgtgga acgggaaaaa ccagtgttgc
     1141 taaaatcttt gccaaggcta tgaactgtcc caatcaagtg ggtggcgaac cttgcaataa
     1201 ctgctatatt tgtcaagcag tgacggacgg tagtttagaa gatgtcattg aaatggatgc
     1261 agcttctaat aatggggtag atgaaattcg cgaaattcgt gataaatcta cctatgcgcc
     1321 tagccttgct cgttataagg tttatatcat agatgaggtt cacatgctgt ctacaggggc
     1381 ttttaatgcc ctcctaaaga cgctggaaga accaacacag aatgtagtct ttattttggc
     1441 cactactgaa ttgcacaaga ttcctgctac tattctatcc cgtgtgcaac gttttgagtt
     1501 taaatcaatt aagacacagg atattaagga acatattcac tatatcttag aaaaagaaaa
     1561 tatcagttct gaaccagagg ctgtggaaat cattgccaga cgggcggaag gtggaatgcg
     1621 ggacgccttg tctattttgg atcaagccct gagtttgaca cagggaaatg agctgacgac
     1681 tgctatctct gaagaaatta ctggcaccat tagcctatca gccttggatg attatgtggc
     1741 ggccttgtct caacaggatg ttcccaaagc tttgtcttgc ttgaatcttc tttttgacaa
     1801 tggtaagagc atgactcgtt ttgtgaccga tcttttgcac tatttaagag acttgttaat
     1861 tgttcaaaca gggggagcaa atactcatca tagttcagtc tttgtagaaa atttggcact
     1921 tcctcaaaaa aatctgtttg aaatgattcg cttagcaaca gtgagtttag cagatattaa
     1981 gtctagtttg caacccaaga tttatgctga aatgatgacc gtccgtttgg cggaaatcaa
     2041 gtccgaacca gctctatcag gagcggttga aaatgaaatt gctacgctga gacaggaagt
     2101 tgcccgtctc aaacaagagc tttctaatgt aggtgcggtt cctaaacaag ttgcaccagc
     2161 tcctagtcga ccagctacgg gcaaaacagt ctatcgtgtc gatcgcaata aagtgcaatc
     2221 tatcttacaa gaggccgtcg aaaatcctga tttagcacgt caaaatttaa ttcgtttgca
     2281 gaatgcctgg ggagaggtaa ttgaaagtct aggtgggccg gacaaggctc tgctagttgg
     2341 ttctcaaccg gttgctgcca atgaacacca tgctattctt gcttttgagt ctaacttcaa
     2401 tgctggtcaa actatgaaac gagacaatct caataccatg tttggtaata tcctcagtca
     2461 ggcggcaggt ttttcacctg agattttagc tatttccatg gaggaatgga aagaagttcg
     2521 cgcagccttt tcagccaaag ccaaatcttc tcaaactgaa aaagaagtag aagaaagcct
     2581 gattccagaa ggatttgaat ttttggctga taaagtgaag gtagaggaag actaaagaaa
     2641 gatttcatga tacaataagt ttatgaataa acaacaattt attattatgg cgctgtttac
     2701 agctgctgag acctattttt tcaatgaagc ctggatgact ggccgctata ttatggcagc
     2761 cttttgggca attttactct ttagaaattt ccgagtcagt tatgtgatgg gcaaaatcgt
     2821 tgatgtcatc gatcagcatt ttaataggaa agactagccc tcagcttcca gacaaaatca
     2881 aagcctttta ggcttttttt tgttatacta gaaaagtata tttatagaat ttttgctcta
     2941 tttctgggga aatcagacgt ttttctagta agtactgtaa aagttttgaa aaagaaagga
     3001 actatcatgt cagtattaga gatcaaagat cttcacgttg agattgaagg aaaagaaatt
     3061 ttaaaagggg ttaacctgac cctgaaaaca ggagaaattg ccgctatcat gggaccaaat
     3121 ggtacaggta aatcgactct ttctgccgct atcatgggaa atccaaacta tgaagtaact
     3181 aaaggtgaag ttttgtttga tggcgtaaac atccttgagt tggaagtgga tgagcgtgcg
     3241 cgtatgggac ttttccttgc tatgcaatac ccatcagaaa tccctggaat taccaatgct
     3301 gagtttcttc gtgccgctat gaatgcgggt aaagaagatg atgagaagat ttcagttcgt
     3361 gagtttatta ctaagctaga tgaaaaaatg gaattgctca acatgaaaga agaaatggca
     3421 gagcgttacc tcaacgaagg cttctctggt ggtgagaaaa aacgcaatga aattcttcaa
     3481 cttttgatgt tggagccaac atttgctctt ttggacgaga ttgactcagg tcttgatatt
     3541 gacgctctta aagttgtgtc taaaggtgtc aatgccatgc gtggtgaagg ttttggtgct
     3601 atgatcatca ctcactacca acgtcttttg aactatatca cacctgatgt ggtacacgtg
     3661 atgatggaag gtcgtgttgt cctttctggt ggtccagaat tggctgcgcg tttggaacgt
     3721 gaaggatacg caaaattagc tgaagaactt ggctacgact acaaggaaga attgtaattc
     3781 cctcgtatct tttaggagaa gtaaatgact agagaaaata ttaaactttt ttcagaaatg
     3841 cacgctgaac caagctggtt ggctgatctc cgtcaaaaag cttttgacaa gattgagact
     3901 ttggaattac cagttattga gtgtgtcaaa ttccaccgtt ggaatctggg tgatggaacg
     3961 attacagaaa atgagccatc agcaaatgtt ccagatttca cagctttaga tcatcacttg
     4021 aagttggtgc aagtaggaac tcaaactgtt ttcgaacaaa ctccagttga gttagctgaa
     4081 cagggtgttg tcttcacaga ctttcactca gctttagaag aaattccaga gctgatcgaa
     4141 gaattcttca tgtcatctgt taagtatgat gatgacaagt tggcggctta ccacacagct
     4201 tactttaaca gtggtgctgt actctatatt ccagataacg tagaaatcac agagccaatt
     4261 gaaggaattt tctaccaaga tagcgatagc aatgtgccgt ttaacaagca tattatgatt
     4321 atcgttggta aaaattctaa gattagttat ctggagcgtt tagagtcacg cggtgaagga
     4381 agtgacaaag caactgccaa tatcacagtg gaagtgattg cacgttctgg tgcgcaagtc
     4441 aagtttgctg ctatcgaccg tctaggtgaa aacgtcactg cctacattag ccgtcgtggt
     4501 aaattaggca acgatgcaag tattgactgg gctatcggtg tcatgaacga aggaaatgtc
     4561 gttgctgatt ttgatagtga cttgattggt aatggtagcc atgctgacct caaggttgta
     4621 gctctttcaa gtggtcgtca ggtacaaggg attgatactc gtgtaactaa ctatggctgc
     4681 aactcaatcg gaaacattct acaacatggg gttatccttg aaaaagcaac tttgactttc
     4741 aatggtatcg gccacatcat caagggtgct aagggagcag atgcgcaaca agagagccgt
     4801 gttctcatgc tttcagacca agcgcgttca gatgctaacc caattctttt gattgatgaa
     4861 aatgacgtaa ctgcaggcca tgcagcctct attggtcagg tagatccaga agatatgtac
     4921 tacctcatga gtcgtggctt ggataaggca actgcagagc gtttggttgt tcgtggtttc
     4981 cttggatctg ttatcgtgga gattccagtc aaggaagttc gtgatgaaat gattgcaact
     5041 atcgaagaga aattgtcaaa acgctaaggg gcagcctatg ttagatgtag aagcgattcg
     5101 caaggatttt ccaattttag atcagattgt caatgatgaa cctctggtct atctggacaa
     5161 tgctgcgacg acacaaaaac cactagtagt tctgaaagct attaacagct actatgagca
     5221 ggacaatgcc aatgttcacc gtggtgtcca taccttagcg gaacgagcga cagcttctta
     5281 tgaagctgct cgtgaaacca ttcgtaagtt tattaatgca ggctctacaa aggaagttct
     5341 ctttaccaga ggaacgacaa ccagccttaa ctgggtggca cgctttgctg aggaaattct
     5401 cactgaggga gaccaggtct tgatttcagt aatggaacac cattctaata tcattccatg
     5461 gcaggaagct tgtcgaaaga ctggagcaga gcttgtctat gtctatctta aagacggtgc
     5521 cttggatatg gaggatttgc gagctaaatt gactgataag gttaaatttg tttccctagc
     5581 tcatgcctcc aatgttcttg gtgtggtcaa tccgatcaag gaaatcactc aattagccca
     5641 ccaagttggg gcaattatgg tagtggatgg tgctcaatct acacctcata tgaagattga
     5701 tgtccaggac ttggatctgg actttttcgc cttttcgggt cacaagatgg ctggtccgac
     5761 tggtatcggt gtcctttacg gcaaagaaaa gtatcttgag caaatgtctc cagtagaatt
     5821 tggcggcgag atgattgatt ttgtctacga gcaatttgct agttggaagg aattgccttg
     5881 gaaatttgag gctggaacgc caaatatggc aggagctatt ggacttgcga ctgcagttga
     5941 ttatctggaa aagattggta tggatgccgt tgaagctcat gaacaggaat tgattgcgta
     6001 cgtctatcca aaactgcagg caattgaggg attgaccatt tacggttctc aggatttggc
     6061 tcaacgttcg ggtgttattg cctttaacct aggtgatctc catcctcacg atcttgcgac
     6121 ggctctggat tatgaaggag tggctgttcg tgctggtcac cattgtgcgc aacccttgct
     6181 tcagtatttg gaagtcccag caacagctcg tgcaagtttt tatatctaca ataccaaggc
     6241 agattgcgac aaactagtcg atgccctaca aaagacaaag gagtttttca atggcacttt
     6301 ctaaactaga tagcctttat atggcagtgg tagcagacca ttcgaaaaat ccacatcacc
     6361 aagggaagtt agaagatgct gagcaaatca gtctcaacaa tccgacttgt ggggatgtca
     6421 tcaacctctc tgtcaagttt gatgcagagg accgtttgga agatattgct tttctaaatt
     6481 caggatgcac gatttcaact gcttctgcta gtatgatgac agatgccgtt ttaggaaaaa
     6541 ccaaacaaga aattttagaa ctggcgacta ttttttctga aatggttcaa gggcaaaaag
     6601 atgagcgtca agaccaactt ggagacgcgg cattcttgtc aggtgttgcc aaattccctc
     6661 aaagaatcaa gtgtgcaacc ctagcttgga atgcccttaa gaaaacaatt gaaaatcaag
     6721 aaaaacagta agacaagttt cttttgtctt atgaattatt agaaatgaag aaagaaagga
     6781 tactatggct gaagaaagag tagaaccaaa accaattgac cttggtgaat ataaatttgg
     6841 tttccatgac gatgtagagc ctgtcttatc gacaggaaaa ggactcaacg aaggtgttat
     6901 tcgtgaatta tctgctgcta agggtgagcc tgagtggatg ttggagttcc gtttgaagtc
     6961 ttatgaaacc ttcaaaaaaa tgcccatgca aacttgggga gcagacttgt cagagattga
     7021 ctttgatgac ttaatctact accaaaaacc atctgacaaa ccagcccgtt cttgggatga
     7081 tgtacctgaa aagattaaag aaacctttga acgtatcggg attccagaag ctgaacgtgc
     7141 ttatttagca ggggcttctg cccagtacga gtcagaagtg gtttaccaca acatgaagga
     7201 agagttccaa aaattaggta ttatctttac agatacagat tccgcactca aggaataccc
     7261 agacttattt aaacaatact ttgcgaagtt ggtaccgccg acagataaca agttggcagc
     7321 cctcaactca gcagtatggt cgggtggaac ttttatctac gtgccaaaag gtgtcaaggt
     7381 agatattcca cttcaaactt atttccgtat caataacgaa aatataggtc agttcgaacg
     7441 taccttgatt atcgttgatg agggagcaag cgtctactac gtagaaggat gtacagcacc
     7501 aacatattca agcaatagct tacacgctgc cattgtagaa atttttgctt tggacggagc
     7561 ttatatgcgt tatacaacta tccaaaactg gtctgataac gtctataact tggtaacaaa
     7621 gcgtgctaag gctcaaaagg atgccactgt tgagtggatt gatggaaact tgggtgccaa
     7681 aacgactatg aaatatccat ctgtttacct tgatggagaa ggagcgcgtg gtaccatgct
     7741 ctctatcgcc tttgctaatg cagggcaaca ccaagacacg ggtgctaaga tgattcacaa
     7801 tgctccacat accagctcgt ctattgtgtc taaatccatc gctaaaggtg gaggaaaggt
     7861 tgactaccgt ggacaagtca cctttaacaa gaactctaag aaatctgttt cccacattga
     7921 atgtgatacc attatcatgg atgacttgtc agcatcagat actattccat ttaatgaaat
     7981 tcacaactcg caagtggctt tggaacacga agccaaagta tctaagattt cagaagagca
     8041 attgtattat ctcatgagcc gtggattgtc agaatctgag gcaactgaaa tgattgtcat
     8101 gggatttgta gaacccttta caaaagaact tccaatggaa tacgcagttg agctgaaccg
     8161 cttgattagc tatgaaatgg agggatcagt tggataaaat ttgattttat actcttcgaa
     8221 aatctcttca aaccacgtca gcatcgcctt accgtatgta tggttactga cttcgtcagt
     8281 ttcatctaca acctcaaaac agtgttttga gcaacctgcg gctagcttcc tagtttgttc
     8341 tttgattttg agtattagat ttactcaaaa tcaaggattt tgaagatgaa cttgtatcaa
     8401 aaaatcgcgg tttaaaatcg cgatttttta taatttctcg ttaacaaagc ggacaaactg
     8461 attccaccaa acttttaaga agaaggcttt ttcaattttc ttgtctgcta ccatttcgaa
     8521 actagggcgc tctgtggtga tgtaaccttg accaatcaag tccttgtctt cataagtcaa
     8581 atggccaacc actgttccag cttcaagtgg tgctgggatt gctttggaat caggtgtgaa
     8641 ttgaacagat tgggaagatt gattcccaac acgttcgatt agatagatat cctctggagc
     8701 cactgcagtt actgtatctt cttttccatc ttgtacaggg gctttgctat cttgataggc
     8761 atcgccttgt tgaacgattt tgcgaagtgt aaatgtagaa gaaatataat ccattaggga
     8821 agatgtagct gtaaatcgag cgtaaggatt attgtcttga tgatctgcat ttaaaacaac
     8881 tgtgatgact ctcatgcctt tttcgacagt agtaccaaca aaagactctc cagccttatc
     8941 tgttgttcct gtttttagcc catcaaaacc accacggtaa gcaggcatac cttctaacat
     9001 gtagttggtt gaagtgattg tcatcccagc aaaagtagaa gaaggttttt tggtgatttc
     9061 taagacttgt gggtattttt tgatgaggtt gcgagcaacg atagcgacat cataagcact
     9121 aagcttattt tcctcatctt ttttagaacc tgggtaaatg ttatccccta gagtttcatt
     9181 gttaagacct gtcgtattga caacagtggc atcctgaatt ccccattcca agagttttgc
     9241 ccgcatcata tcgacgaaat ctttttctga gccagcaatt ttctcagcta gggcaatagc
     9301 ggcgctgttg gcactagata ccagagttgc ttcaagcaac tcttcgacag tataattacg
     9361 ggcctccata ggaatattac tggcttcaga atttgtcgtc aattgataag gataatcaga
     9421 aatatctaca ggagtggaga gggtaatact tccgttttcc aaagcttcat agaccagata
     9481 aacagtaatc aattttgtta tggaagcaat ttcgacaggt tgcgttgcat ccttctcata
     9541 gagaatttta ccagtatttg cctcaacagc aatcgcatgt ttagcggcaa tggtaaaatc
     9601 ttgagcaaca gcagtagaag caccccctaa aagagagaca gttaacaaag ttaaaaatat
     9661 ttttttcata gtagtcttat tctatcataa agaaaaaaaa tattcttgct ttaataattc
     9721 atctgttaag ctttttgaaa atatggtaaa ataaagtaag ggaggtaact catgtttcgt
     9781 agaaataaat tatttttttg gaccacagaa attttactct taaccatcat cttttaccta
     9841 tggagacaga tggggtcttt gattaaccct tttgttagcg tgcttaatac aattatgatt
     9901 ccatttttat tagggggctt tttttattat ttgacaaacc ctattgttac tttcttaaat
     9961 aaagtctgta aactcaatcg tttgcttggt attttaatta ccttgtgtac tttggtctgg
    10021 ggaatggtca taggtgttgt ctatctctta cctattttga ttaatcagtt atctagtttg
    10081 attatatcta gtcaaactat ttatagtcga gtacaagact taatcataga cttatctaat
    10141 tatcctgcgc tccagaattt ggatgtagaa gctacaattc agcagttaaa cttatcctat
    10201 gttgatattc ttcaaaatat cctaaatagc gtatcaaata gtgtggggag cgtcttgtca
    10261 gctcttatca gtactgtttt gattttgatt atgactccag tttttttggt ttatttctta
    10321 ttagatggac ataaattctt gcccatgctt gaaagaacga ttctaaagag ggatcgcttg
    10381 catattgcag gcttattaaa gaatttaaat gcgacgattg ctcgctatat tagtggagtt
    10441 tcgattgacg caatcattat aggttgtttg gcttatattg gctatagtat tattggttta
    10501 aaatatgctt tagtttttgc cattttttct ggtgtagcca atttaattcc ttatgtgggg
    10561 ccaagtattg gtttgattcc tatgatcatc gcaaatatat tcactgatcc ccatagactg
    10621 ctgattgcag tgatttatat gcttgttgtt cagcaggtag atggcaatat cttatatcct
    10681 cgaatcgtag gaagtgttat gaaggttcat ccaatcacga ttttagtttt acttttgttg
    10741 tcaagcaata tctatggtgt agttggaatg attgtcgcag tgccaaccta ttctatcttg
    10801 aaagaaattt ctaagttctt atcccatttg tatgaaaatc ataaaataat gaaagaacga
    10861 gaaagagaat tagctaagta aaagtcagga gaaccctgat ttttctttac tggaagtggc
    10921 ctttagatta gaagactgaa aataagttaa agtcttaaac taattttcac agctaagaat
    10981 agtagaagtt aatctgataa aaatcgaaaa aaccagtgga attctgtgtc agggtaagtt
    11041 ccactggttt tcatagtcta ttaaagttcg aatgaaacct atttatagta gattgaaact
    11101 agaatagtac acctctaatt ctaaaacatt gttagaaatc gatttgactg tcctgatcta
    11161 ttcgttctat tcttatttca ttttactata ttttggtgca ataagtgaaa agtagtccga
    11221 ataatataag gattgatttt atagttttta aactcaaatg aattgaaata aagagagtac
    11281 gaaaattctc atctgaaagt attttagaat aattctcttc gtgaatttct tcaaaacaga
    11341 tagcttcatc ttaggtatgt gatttctttt tgcatttttg agttagataa ggtataatga
    11401 ttttattgtc ttttggggtc gttacggatt cgacaggcat tatgaggcat attttgcgac
    11461 tcgtgtggcg acgtaaacgc tcagttaaat ataactgcaa aaaataacac ttcttacgct
    11521 ctagctgcct aaaaaccagc aggcgtgacc cgatttggat tgctcgtgtt caatgacagg
    11581 tcttattatt agcgagatac gattaagcct tgtctagcgg tttgataaga gattgataga
    11641 ctcgcagttt ctagacttga gttatgtgtc gaggggctgt taaaataata cataacctat
    11701 ggttgtagac aaatatgttg gcaggtgttt ggacgtgggt tcgactccca ccggctccat
    11761 tattcctttg cattcttttg cattccttgg taaaacgttg ttaaatcaac gttttttatt
    11821 tttatctttg gtattccttt gcattctttt gctaaaaagg gagtcacaaa cagaccctat
    11881 tttaaaaaaa ggatagaaaa aaggatacaa catttgtcgc atcctaaaaa taatcttttt
    11941 tcgacggaag acatgggatt cgaacccacg cacgctatta cacgcctacc gcgtttccaa
    12001 cacggcctct taagcctctt gagtaatctt ccaatactta ctcaaatagt ctaccataaa
    12061 ggctcttatc ttgcaataaa aattctagaa ataagaaaaa tgatagattt tgaaagaaaa
    12121 tgataaaaaa tgcttgactt cgaaagaaag tatgatagaa tgaatagtgt aaacgataac
    12181 aggaggtgat tcagtgttaa aaacagaacg taaacaacta attttagagg agttaaatca
    12241 acatcatgta gtttctctag aaaaattagt tagtttgcta gaaacgtcag aatcaacggt
    12301 tcgaagagac ttggatgagt tggaagcgga aaacaagctt cgtcgtgtgc atggtggagc
    12361 agaactcccc tactccttac aggaagaaga aaccattcaa gaaaaatctg tcaaaaacct
    12421 tcaagaaaag aaattgctgg ctcagaaagc agcctctctc attaaagaaa aagatgtcat
    12481 ctttatcgat gctggaacaa caactgcttt tttgattcat gaattggtca ataagaatgt
    12541 tacagttgtg accaactcca ttcaccatgc cgctcagttg gttgaaaagc agattccaac
    12601 tgtcatggtt ggaggaaacg tcaagacggc gacagatgct agtatcgggg gcgttgctct
    12661 taaccagatt aaccaattgc actttgaccg tgcctttatc ggaataaatg gtgttgacga
    12721 tggctattat acgactcctg atatggagga gggagctgtg aaaagagcta ttttggagaa
    12781 tgccaagcag acctacgtct tggtggattc gtcaaaaatt ggacaaactt gctttgccaa
    12841 ggtagcccca ctcaaacgcg ctatcgttat cactagtcaa gggcatgagc tcttgcaggt
    12901 tattaaggag aaaacggagg taatagaagt atgatttata cagtcacact caatccatcc
    12961 attgactata tcgttcgttt ggaccaagtc aaagttggta gtgtcaatcg tatggacagt
    13021 gatgataagt ttgctggtgg gaaaggaatc aatgtcagcc gtgtcttgaa acgtttgaat
    13081 ataccaaata cagcgacggg atttatcggt ggctttactg gtaaatttat cacagatact
    13141 ttagcagagg aagaaatcga gacacgtttt gtccaggtgg cagaagatac tcgtatcaat
    13201 gttaaaatca aagcagacca agaaacagaa atcaacggaa cgggtccaac tgttgaatcg
    13261 gttcagctag aagaattgaa agctatttta tctagtctga cagcagaaga tacagttgtc
    13321 tttgcaggtt caagtgctaa aaatctaggc aatgttatct ataaggattt gatttccttg
    13381 acgcgccaga ctggtgcgca agtggtctgt gactttgaag gacagacctt aattgatagt
    13441 ttggactacc agcctcttct tgtaaaacca aacaatcatg aacttggagc gatttttggg
    13501 gttaaactcg aaagtttaga tgaaattgag aaatacgctc gtgagttact ggctaagggt
    13561 gctcaaaatg ttattatctc tatggctggt gatggtgccc ttcttgtcac atctgaggga
    13621 gcttacttcg ctaaaccaat caaaggaaca gtcaaaaatt cagttggagc tggtgattct
    13681 atggttgctg gattcacagg tgaatttgtc aaatcaaaag acgtagtaga agccttcaaa
    13741 tggggagtgg cttgcggaac ggcaactacc ttctcagatg acttggcaac ggcggaattt
    13801 attaaagaaa catatggaaa agttgaggta gaaaaacgat gaaaattcaa gacctattga
    13861 gaaaagatgt catgttgcta gatttgcagg caactgaaaa aacagctgtc atcgacgaga
    13921 tgattaaaaa tttgacagac cacggttatg taacagattt tgaaacattt aaagaaggaa
    13981 ttttggcgcg tgaagctttg acttctactg gtttgggtga tggaatcgca atgcctcaca
    14041 gcaaaaacgc tgctgtcaaa gaagcgacag ttctatttgc taagtcaaat aagggtgttg
    14101 actacgagag cttggatgga caagcaactg acctcttctt catgattgca gctccagaag
    14161 gtgccaatga tactcacttg gcagccttgg cagaattgtc tcaatacttg atgaaagacg
    14221 gttttgcaga caaacttcgt caagcaacat ctgcagacca agttatcgaa ctttttgacc
    14281 aagcttcaga aaaaactgag gaacttgttc aagcacctgc taatgactct ggtgacttta
    14341 tcgtagctgt tacagcttgt acaacaggta ttgcccacac ttacatggcc caagaagccc
    14401 ttcaaaaagt agctgctgaa atgggggttg gtatcaaggt cgaaaccaac ggtgctagcg
    14461 gtgttggaaa tcaactaact gcagaagata tccgtaaggc taaagctatt atcattgcag
    14521 cagacaaggc cgttgaaatg gatcgatttg atggaaaacc attgatcaat cgtccagttg
    14581 ctgacggtat ccgtaagaca gaagagctaa ttaacttggc tctttcagga gatactgaag
    14641 tctaccgtgc cgctaatggt gccaaagctg caacagcctc taacgaaaaa caaagccttg
    14701 gtggtgcctt gtacaaacac ttgatgagtg gtgtatctca aatgttacca ttcgttatcg
    14761 gtggtggtat catgattgcc cttgccttct tgattgacgg tgctttgggt gttccaaatg
    14821 aaaaccttgg caatcttggt tcttaccatg agttagcttc tatgttcatg aaaattggtg
    14881 gagctgcctt tggtttgatg cttccagtct ttgcgggtta tgttgcctac tctattgctg
    14941 aaaaaccggg tttggtagca ggtttcgtgg ctggtgctat tgccaaagaa ggttttgcct
    15001 ttggtaaaat tccttatgcc gcaggtggtg aagcaacttc aactcttgca ggtgtctcat
    15061 ctggtttcct aggtgccctt gttggtggat ttatcgcagg tgccttggtt cttgccatca
    15121 agaaatacgt taaagttcct cgttcactcg aaggtgctaa atcaatcctt ctattgccac
    15181 ttcttggaac aatcttgaca ggatttgtta tgctagctgt gaatatccca atggctgcaa
    15241 tcaacactgc tatgaatgac ttcctaggcg gtcttggagg aggttcagct gtccttcttg
    15301 gtatcgtcct tggtggaatg atggctgttg acatgggtgg accagttaat aaagcagctt
    15361 atgtctttgg tacaggtacg cttgcagcaa ctgtttcttc aggtggttct gtagccatgg
    15421 cagcagttat ggctggagga atggtgccac cacttgcaat ctttgtcgca actcttcttt
    15481 tcaaagataa atttactaag gaagaacgta actctggttt gacaaacatc atcatgggct
    15541 tgtcatttat cactgaggga gcgattccat ttggtgccgc tgacccagct cgtgcgattc
    15601 caagcttcat ccttggttca gcagtagcag gtggactcgt tggtcttact ggtatcaaac
    15661 tcatggcgcc acacggagga atcttcgtta tcgcccttac ttcaaatgct ctcctttacc
    15721 tcgtttctgt cttggtagga gcaatcgtaa gtggtgtggt ttatggttac ctacgcaaac
    15781 cacaagcata aaaaatagaa aaatgaaaag attggaccgt ttggtgcagt ctttttctct
    15841 tcccgaaatg cctgtgaaat atggtataat agaagaatgg caaacaagaa tacaagtaca
    15901 acaagacgga gaccgtctaa agcagaactg gaaagaaaag aagcgattca acgaatgttg
    15961 atttcgttag gaattgcgat tttattgatt ttcgcagcct tcaaattagg ggctgcaggt
    16021 ataacccttt ataatttaat tcgcttgcta gtgggtagcc tagcttatct ggcgatattc
    16081 ggcctattaa tctatctctt ctttttcaag tggatacgaa aacaggaagg actcttatct
    16141 ggctttttca ccatatttgc tggcttactc ttgatttttg aggcctactt ggtttggaaa
    16201 tatggtttgg acaagtccgt tctaaaaggg accatggctc aggttgtgac agatctgact
    16261 ggttttcgaa cgactagctt tgctggaggg ggcttgatcg gggtcgctct ttatattcca
    16321 acagcctttc tcttttcaaa tatcggaact tactttattg gttctatctt gattttagtg
    16381 ggttctctcc tagtcagccc ttggtctgtt tacgatattg ctgaattttt cagtagaggc
    16441 tttgccaaat ggtgggaagg gcacgagcgt cgaaaagagg aacgctttgt caaacaagaa
    16501 gaaaaagctc gccaaaaggc tgagaaagag gctagattag aacaagaaga gactgaaaaa
    16561 gccttactcg atttgcctcc tgttgatatg gaaacgggtg aaattctgac agaggaagct
    16621 gttcaaaatc ttccacctat tccagaagaa aagtgggtgg aaccagaaat catcctgcct
    16681 caagctgaac ttaaattccc tgaacaggaa gatgactcag atgacgaaga tgttcaggtc
    16741 gatttttcag ccaaagaagc ccttgaatac aaacttccaa gcttacaact ctttgcacca
    16801 gataaaccaa aagatcagtc taaagagaag aaaattgtca gagaaaatat caaaatctta
    16861 gaagcaacct ttgctagctt tggtattaag gtaacagttg aacgggccga aattgggcca
    16921 tcagtgacca agtatgaagt caagccggct gttggtgtaa gggtcaaccg catttccaat
    16981 ctatcagatg acctcgctct agccttggct gccaaagatg tccggattga agcaccaatc
    17041 cctgggaaat ccctaatcgg aattgaagtg cccaactccg atattgccac tgtatctttc
    17101 cgagaactat gggaacaatc gcaaacgaaa gcagaaaatt tcttggaaat tcctttaggg
    17161 aaggctgtta atggaaccgc aagagctttt gacctttcta aaatgcccca cttgctagtt
    17221 gcaggttcaa cgggttcagg gaagtcagta gcagttaacg gcattattgc tagcattctc
    17281 atgaaggcga gaccagatca agttaaattt atgatggtcg atcccaagat ggttgagtta
    17341 tctgtttaca atgatattcc ccacctcttg attccagtcg tgaccaatcc acgcaaagcc
    17401 agcaaggctc tgcaaaaggt tgtggatgaa atggaaaacc gttatgaact ctttgccaag
    17461 gtgggagttc ggaatattgc aggttttaat gccaaggtag aagagttcaa ttcccagtct
    17521 gagtacaagc aaattccgct accattcatt gtcgtgattg tggatgagtt ggctgacctc
    17581 atgatggtgg ccagcaagga agtggaagat gctatcatcc gtcttgggca gaaggcgcgt
    17641 gctgcaggta tccacatgat tcttgcaact cagcgtccat ctgttgatgt catctctggt
    17701 ttgattaagg ccaatgttcc atctcgtgta gcatttgcgg tttcatcagg aacagactcc
    17761 cgtacgattt tggatgaaaa tggagcagaa aaacttcttg gtcgaggaga catgctcttt
    17821 aaaccgattg atgaaaatca tccagttcgt ctccaaggct cctttatctc ggatgacgat
    17881 gttgagcgca ttgtgaactt catcaagact caggcagatg cagactacga tgagagtttt
    17941 gatccaggtg aggtttctga aaatgaagga gaattttcgg atggagatgc tggtggtgat
    18001 ccgctttttg aagaagctaa gtctttggtt atcgaaacac agaaagccag tgcgtctatg
    18061 attcagcgtc gtttatcagt tggatttaac cgtgcgaccc gtctcatgga agaactggag
    18121 atagcaggtg tcatcggtcc agctgaaggt accaaacctc gaaaagtgtt acaacaataa
    18181 aaaaatagct tctttccaag tttggaggga agctatttta gtggctattg attgctttta
    18241 ttttctgaag ttggcgcatt ggactgtttt tcgttttcag tagcaggttt acttgaagca
    18301 ggagtagaag agtcctgagt tgctgttttc tgatcttctt ttttctcttc cttgacgcta
    18361 gattttggtg tttcctcttg ctgtgttttt tcttgactag tgttagtctc tttagttgga
    18421 ctggtgtttt ccttagggga ttccttttgg atttctttga caatggttgt cgtctggctt
    18481 gtcgtaggtt cttttttaat atttttgtta ttatccaagg cgttcataat cgtgatactt
    18541 gcggtaatca agccaaggat actagcgatt gtagcaacgg ttttttgaaa gactgtaaag
    18601 ccttttttga tgtgttctgt ttttggtttt gaagttctac gatagttaga catacattct
    18661 ctcctttgat tatgatttat tatactctgt ttctttaaaa aaatcttaaa aaaagagaaa
    18721 tttccctttc ttgtcgcaag ctccgtttca tgatacaata gaggaagtga ttttagaaag
    18781 cgtgagaaac atgatttact ttgataattc agcgacgacc aagccttatc ctgaagccct
    18841 tgaaacctat atgcaggtcg cttcaaaaat tttaggaaac ccatctagtc tccatcgttt
    18901 gggagaccaa gcaacacgaa tcttagatgc ttctcgccaa cagattgcag atttaatcgg
    18961 taagaaaagc gatgaaatct tctttacctc gggtggaaca gaaggggata actggcttat
    19021 caagggtgtg gcctttgaaa aagctcagtt tggcaagcac atcattgttt cagccattga
    19081 acatccagca gtcaaagagt cagccctctg gttgaaaagt caaggatttg aagtggattt
    19141 tgctccagtt gataagaaag gcttggtcga tgttgaggcg ttagcaggtt tgatacggca
    19201 tgatacaatc ctcgtttcca tcatggctgt gaacaatgaa atcggctcta tccaacctat
    19261 tgaggctatt tcagaattct tggcagacaa gccgactatt tccttccacg ttgatgcggt
    19321 tcaggcgctt gccaaaattc cgactgaaaa gtatctgaca gaacgggtgg attgcgcgac
    19381 tttctctagt cacaagttcc acggggttcg aggtgttggc tttgtctata tcaaatctgg
    19441 caagaagatt acacctcttc ttacaggtgg tggccaggag cgagattatc gttcgacaac
    19501 tgaaaatgtg gcagggattg cagcgacagc caaggccctc cgtttgtcta tggaaaagct
    19561 agatatcttt aggagcaaga ctgggcagat gaaggcagtg attcgccaag ctcttctgaa
    19621 ctatccggat atttttgtct tttcagatga ggaaaacttt gcacctcata ttctgacttt
    19681 tggaatcaaa ggtgttcgag gtgaagtcat cgttcacgcc tttgaagact atgatatttt
    19741 catctcaaca acctcagctt gttcatctaa ggcaggaaaa ccagccggta ccttgattgc
    19801 catgggagtg gacaaagata aggccaagtc agctgtgcgt cttagcctag acttggaaaa
    19861 tgatatgagt caggtcgagc agtttttgac caagttaaaa ttgatttaca atcaaactag
    19921 aaaagtaaga taggagcatt catgcagtat tcagaaatta tgattcgcta cggagagttg
    19981 tcaaccaagg gtaaaaaccg tatgcgtttc atcaataaac ttcgtaataa tatttcggac
    20041 gttttgtcta tctataccca agttaaggta acagcagatc gcgaccgtgc ccacgcttac
    20101 ctcaatggag ctgattacac agcagttgca gaatctctca aacaagtttt tggaattcaa
    20161 aacttttctc ctgtttataa ggttgaaaaa tctgtagaag ttttgaagtc ttctgtccaa
    20221 gagattatgc gggacatcta caaggaaggt atgaccttta agatttctag caagcgtagc
    20281 gaccacaact ttgaacttga tagtcgtgaa ctcaaccaaa cacttggagg ggctgtattc
    20341 gaagccattc caaatgtgca agttcaaatg aaaagtcctg acatcaatct tcaggtggag
    20401 attcgtgaag aagcagccta tctttcttat gaaaccattc gtggggctgg tggtttgcca
    20461 gttggaactt caggtaaagg gatgctcatg ttgtcaggag ggattgactc acctgtagca
    20521 ggttatcttg ctcttaagcg tggggtggat atcgaggcag ttcactttgc tagtccacca
    20581 tatactagtc ctggtgccct caagaaagcg caggacttga cccgtaaatt gaccaagttt
    20641 ggcggaaata tccagtttat agaggtgcct ttcacagaga ttcaagagga aatcaaagcc
    20701 aaagcgccag aagcttattt gatgactcta actcgtcgct ttatgatgcg gattactgac
    20761 cgtattcgtg aggtacgaaa tggtttggtt atcatcaatg gggaaagtct aggtcaagta
    20821 gccagccaaa cccttgaaag tatgaaggct atcaatgctg ttaccaacac tcccatcatt
    20881 cgtcctgtgg ttaccatgga caagttggaa atcattgaca tcgcccagga aatcgatacc
    20941 tttgacattt caatccaacc gtttgaagac tgttgtacca tttttgcacc agatcgtcca
    21001 aaaacaaatc ctaaaattaa gaatgcggag cagtacgaag cgcgtatgga tgttgaaggc
    21061 ttggttgagc gagcagtggc tggaatcatg attactgaaa tcacacctca agccgaaaaa
    21121 gatgaagttg atgacttgat tgacaatctg ctctaattca gaaaatccaa aagaatagcg
    21181 aaaatcagta aaaaaagtta gttttttctc taaaaacagg taaaaaacta acttttttta
    21241 tttttattat ataatgatat aaaattttga atatagagag ttttctgaca atgaatcaat
    21301 cctactttta tctaaaaatg aaagaacaca aactcaaggt tccttataca ggtaaggagc
    21361 gccgtgtacg tattcttctt cctaaagatt atgagaaaga tacagaccgt tcctatcctg
    21421 ttgtatactt tcatgacggg caaaatgttt ttaatagcaa agagtctttc attggacatt
    21481 catggaagat tatcccagct atcaaacgaa atccggatat cagtcgcatg attgtcgttg
    21541 ctattgacaa tgatggtatg gggcggatga atgagtatgc ggcttggaag ttccaagaat
    21601 ctcctatccc agggcagcag tttggtggta agggtgtgga gtatgctgag tttgtcatgg
    21661 aggtggtcaa gccttttatc gatgagacct atcgtacaaa agcagactgc cagcatacgg
    21721 ctatgattgg ttcctcacta ggaggcaata ttacccagtt tatcggtttg gaataccaag
    21781 accaaattgg ttgcttgggc gttttttcat ctgcaaactg gctccaccaa gaagccttta
    21841 accgctattt cgagtgccag aaactatcgc ctgaccagcg catcttcatc tatgtaggaa
    21901 cagaagaagc agatgataca gacaagacct tgatggatgg caatatcaaa caagcctata
    21961 tcgactcgtc gctttgctat taccatgatt tgatagcagg gggagtacat ctggataatc
    22021 ttgtgctaaa agttcagtct ggtgccatcc atagtgaaat cccttggtca gaaaatctac
    22081 cagattgtct gagatttttt gcagaaaaat ggtaagttaa gaaaggaaaa aacgaaatgc
    22141 atattgaaca tcttagccac tggagtggtc atcttaaccg tgaaatgtac cttaaccgtt
    22201 atggacatgg tgggattcca gttgtggtct ttgcttcatc aggtggtagt cacaacgaat
    22261 actatgattt tggcatgatt gatgcctgtg cttcctttat cgaggaaggc cttgtccagt
    22321 tctttaccct atctagtttg gatagtgaga gctggttggc tacttggaaa aatgctcatg
    22381 accaagcgga aatgcaccgt gcctacgaac gttatgtgat tgaggaggcc attcttttat
    22441 caagcacaag acaggttggt ttgatggcat gatgacgaca ggttgctcta tgggagccta
    22501 tcatgcactc aatttcttcc tccagcatcc agatgtcttt accaaagtga ttgctctcag
    22561 tggtgtttac gacgcacgtt tctttgtcgg tgattactac aacgatgatg ctatttacca
    22621 aaactcgcca gtagattata tttggaacca aaacgacggc tggtttattg accgttaccg
    22681 tcaggcagag attgtgctgt gtacggggct tggagcctgg gaacaagatg gtttgccatc
    22741 cttttacaag ctcaaagaag cctttgacaa gaaacaaatt ccagcctggt ttgctgaatg
    22801 gggacatgat gtcgcccatg actgggaatg gtggcgtaaa caaatgcctt atttcctcgg
    22861 taatctctat ttataaaagg agttacctat gaattacctt gttatttctc cctactatcc
    22921 acaaaacttt caacagttta ccatcgaact agctaataaa ggcatcacag tcttgggaat
    22981 tggtcaagag tcttacgagc aattggatga gcccttgcgc aatagcttga ccgagtattt
    23041 tcgtgttgat aatcttgaga acatagatga agtcaaacgt gcagttgctt ttctctttta
    23101 taaacatggt ccaattggcc gcatcgagtc tcacaatgaa tactggcttg agctagacgc
    23161 aacactcaga gaacaattca atgtttttgg tgccaaacca gaggatctca aaaagacgaa
    23221 atataagtct gaaatgaaga aacttttcaa aaaagcaggt gttcctgtgg tacctggagc
    23281 tgttatcaag acggaagcag atgttgatca agcagtgaaa gaaatcggtc ttccaatgat
    23341 tgccaaacct gataatggag tgggagcagc cgcaaccttt aaacttgaga cagaagacga
    23401 tatcaatcac ttcaagcaag aatgggacca ttcaaccctt tatttctttg aaaaatttgt
    23461 cacttccagc gaaatctgta cctttgacgg gctcgtggac aaggatggaa agattgtctt
    23521 ctcaacaacc tttgactacg cctatacacc gcttgacctc atgatttata agatggacaa
    23581 ttcttattat gtgctcaagg atatggatcc taaactgcgc aagtatgggg aagcaattgt
    23641 caaagaattt ggtatgaaag aacggttttt ccatattgag ttcttccgtg agggggacga
    23701 ttatattacc atcgagtaca ataaccgccc tgcaggtggt tttaccattg atgtttataa
    23761 ctttgctcat tccttggacc tttatcgtgg ctatgcagct attgtcgcag gagaggagtt
    23821 cccggcgtca gactttgaaa ctcagtattg tttggctact tctcgccgtg caaatgctca
    23881 ctatgtttat tcagaagagg atttgcttgc caaatatagc cagcagttca aggttaaaaa
    23941 agtcatgcca gctgccttcg cggaacttca aggagattac ctgtatatgc tgaccactcc
    24001 gagtcgacaa gaaatggagc agatgattgc agatttcgga caacgtcaag aataagaact
    24061 atcggattaa ggaaattaac tcccttaatc cttttgtttt gtctgataaa aaataagagc
    24121 atcccaacaa ggtagctatc ataaaacttg ttcgataact atttgaagca ggattaggtg
    24181 gtcagaaatt aaattttaat atttcaattg agtcatagta ttgtgtttgc gtatccttaa
    24241 atcagctaaa aggatccatg acgacaccta tacgatatag ttttcaagat accaaacaag
    24301 tctattaata ttcaatgaaa atcaaagagc aaactaggaa gctagccgca ggtt
//
LOCUS       DBDESG010000028        24134 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.028, whole genome shotgun sequence.
ACCESSION   DBDESG010000028 DBDESG010000000
VERSION     DBDESG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.028"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(417..1907)
                     /gene="lysS"
                     /locus_tag="ACKB38_001137"
     CDS             complement(417..1907)
                     /gene="lysS"
                     /locus_tag="ACKB38_001137"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGL7979913.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(2047..2181)
                     /locus_tag="ACKB38_001138"
                     /pseudo
     CDS             complement(2047..2181)
                     /locus_tag="ACKB38_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            2407..3075
                     /locus_tag="ACKB38_001139"
     CDS             2407..3075
                     /locus_tag="ACKB38_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7979914.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            3035..3712
                     /locus_tag="ACKB38_001140"
     CDS             3035..3712
                     /locus_tag="ACKB38_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7979915.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILILYFAVCYPISKLSTH
                     LEKHWRN"
     gene            3713..4471
                     /locus_tag="ACKB38_001141"
     CDS             3713..4471
                     /locus_tag="ACKB38_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7979916.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            4483..4725
                     /locus_tag="ACKB38_001142"
     CDS             4483..4725
                     /locus_tag="ACKB38_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HGL7979917.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYATILN"
     gene            4767..5354
                     /locus_tag="ACKB38_001143"
     CDS             4767..5354
                     /locus_tag="ACKB38_001143"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGL7979918.1"
                     /translation="MSVDAANRAEYLISNKVDITLANFTVTDERKKQVDFALPYMKVS
                     LGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLD
                     GRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKL
                     GKENFFHKAYEKTLHPTYGDAAKADDLVVEGGKVD"
     gene            complement(5441..6088)
                     /locus_tag="ACKB38_001144"
     CDS             complement(5441..6088)
                     /locus_tag="ACKB38_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979919.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(6085..6783)
                     /locus_tag="ACKB38_001145"
     CDS             complement(6085..6783)
                     /locus_tag="ACKB38_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979920.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(6793..7544)
                     /locus_tag="ACKB38_001146"
                     /pseudo
     CDS             complement(6793..7544)
                     /locus_tag="ACKB38_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7544..8308)
                     /locus_tag="ACKB38_001147"
     CDS             complement(7544..8308)
                     /locus_tag="ACKB38_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979921.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(8404..8526)
                     /locus_tag="ACKB38_001148"
     CDS             complement(8404..8526)
                     /locus_tag="ACKB38_001148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979922.1"
                     /translation="MYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWNPDSY"
     gene            complement(8987..9619)
                     /gene="pyrE"
                     /locus_tag="ACKB38_001149"
     CDS             complement(8987..9619)
                     /gene="pyrE"
                     /locus_tag="ACKB38_001149"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGL7979923.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(9653..10354)
                     /gene="pyrF"
                     /locus_tag="ACKB38_001150"
     CDS             complement(9653..10354)
                     /gene="pyrF"
                     /locus_tag="ACKB38_001150"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGL7979924.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            10673..10897
                     /locus_tag="ACKB38_001151"
                     /pseudo
     CDS             10673..10897
                     /locus_tag="ACKB38_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006269888.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11100..11813)
                     /locus_tag="ACKB38_001152"
     CDS             complement(11100..11813)
                     /locus_tag="ACKB38_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979925.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSFSLAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEEFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(11806..12645)
                     /locus_tag="ACKB38_001153"
                     /pseudo
     CDS             complement(11806..12645)
                     /locus_tag="ACKB38_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(12849..13229)
                     /locus_tag="ACKB38_001154"
     CDS             complement(12849..13229)
                     /locus_tag="ACKB38_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979926.1"
                     /translation="MKDNNIRAKTFIRSVCFCLLSGGVAFLSAIGQFTVIETQLIVLF
                     LGIIFALYYAYYNKNIQTSLENIVWLFSSFEILFLLVNFRTFIQLPVDIFIGMIIFLM
                     LWIFIMLGIVCLSYYITLLFSKEA"
     gene            complement(13226..14032)
                     /locus_tag="ACKB38_001155"
     CDS             complement(13226..14032)
                     /locus_tag="ACKB38_001155"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HGL7979927.1"
                     /translation="MNEQILISDEIDIDSRYSRTKGYYSLFYNEEYNKIQNKTVLVLG
                     AGVLGCYISLSLSMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSEKIHK
                     YNSDVQVVPISIKVSSVEELEKIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKISY
                     ISGGFNGCYLIIDNIYIPTIGSCFGCRNINKDINKYTLSDKTKWPTTPEMPAILGGIM
                     TNLIIKIFLGCYNEILIDNAYVYNMRNHALSQEKYVLENGECPICKKIIK"
     gene            complement(14204..14840)
                     /locus_tag="ACKB38_001156"
                     /pseudo
     CDS             complement(14204..14840)
                     /locus_tag="ACKB38_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(15200..15322)
                     /locus_tag="ACKB38_001157"
     CDS             complement(15200..15322)
                     /locus_tag="ACKB38_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979928.1"
                     /translation="MQDNFLFEEIEEISVPVNDFSAGLATGIGFGLAILALAGC"
     gene            complement(15343..15468)
                     /locus_tag="ACKB38_001158"
     CDS             complement(15343..15468)
                     /locus_tag="ACKB38_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979929.1"
                     /translation="MSDEVFLFEEIEEIVAPTDGEFLGEVLLGTGVVLLIGVACC"
     gene            complement(15599..16798)
                     /gene="divIB"
                     /locus_tag="ACKB38_001159"
     CDS             complement(15599..16798)
                     /gene="divIB"
                     /locus_tag="ACKB38_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGL7979930.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLFVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(16808..17866)
                     /locus_tag="ACKB38_001160"
     CDS             complement(16808..17866)
                     /locus_tag="ACKB38_001160"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGL7979931.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKCGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(17868..19220)
                     /gene="murD"
                     /locus_tag="ACKB38_001161"
     CDS             complement(17868..19220)
                     /gene="murD"
                     /locus_tag="ACKB38_001161"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGL7979932.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(19324..19965)
                     /locus_tag="ACKB38_001162"
     CDS             complement(19324..19965)
                     /locus_tag="ACKB38_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979933.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(19967..21988)
                     /locus_tag="ACKB38_001163"
     CDS             complement(19967..21988)
                     /locus_tag="ACKB38_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGL7979934.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(22059..22358)
                     /locus_tag="ACKB38_001164"
                     /pseudo
     CDS             complement(22059..22358)
                     /locus_tag="ACKB38_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            complement(22722..22976)
                     /locus_tag="ACKB38_001165"
     CDS             complement(22722..22976)
                     /locus_tag="ACKB38_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGL7979935.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(22996..>24134)
                     /locus_tag="ACKB38_001166"
     CDS             complement(22996..>24134)
                     /locus_tag="ACKB38_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGL7979936.1"
                     /translation="VKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAGDLI
                     AVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSRKVE
                     ERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEVIVK
                     EIDGIKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPARGL
                     IGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMSIEE
                     RGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPRILT
                     LEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
BASE COUNT         7691 a         4946 c         4032 g         7465 t
ORIGIN      
        1 tatttgctga ttctatctac taatactata aaaaacaagt gaataagaca ccagaacatc
       61 ctctaatagg acctatggac ttatatcaat cccatatata ctgtttaagc aagatactat
      121 cctagttgac gctaacttcc tatcccactt attaattttt tatttatagt gaatctgaac
      181 aaatcaattg gggaatccaa gtcaatttct agtacttttt gaaaagtttc agcgtactat
      241 cgtaaaatga aataaaacat gcacaaattg cttagggaat ttaaaccaat ttctaacaat
      301 gtcttagaaa tcaaagtgta ctatttcaat ttcaatatac tacacttcat tttctcctta
      361 aattcagtct ctttttgaat caaaaaatcc tctgataaga cccagaggat aagaatttat
      421 ttcattgttg ggaagagcaa tacatcacgg atagttgttg tatcagtgag gagcatgcag
      481 agacggtcga taccgattcc caaaccacct gttggtggca taccgtattc aagagcttca
      541 atgtagtcat agtcgattcc tgtcgcttca tcatcaccaa gttctttggc tttagcttgg
      601 gcttcaaaac ggctaagttg gtcgattggg tcgttcaact cagtaaaggc attaccgtac
      661 tccttagtca tgataaagag ctcgaaacgg tcagtaaagc gttggtcttc aggatttttc
      721 ttagcgagtg gagatacagc tactggatgt ccatagacaa aggttggttg gattaaagtt
      781 tcttcaacaa actcttcaaa gaaggcattg atgatgtgac caacctcagt gtagtgtttc
      841 tcaactggaa ctttcttctc agcagcgata gctttagctt cttccaaagt catgtcttgc
      901 cagaaatcga caccagtaat ttctctgata gcatccacca tatgaacacg cttaaatggt
      961 tcgttaatct tgatttcagt accttggtag ttgactgggc catcaccttt gactgattta
     1021 gcagcgtgtt ggataatgcc ttcagtcaag tccatgatgt cttggaagtc tgcataagct
     1081 tggtaaactt cgatagaagt gaactcaggg ttatgagtag cgtccattcc ttcgttacgg
     1141 aagatacggc caatttcata gacacgttcc ataccaccca cgataaggcg ttttaagtga
     1201 agctcagtcg cgatacgaag caccatgtca atgttttggg cattgtggtg ggtgataaat
     1261 ggacgggcag cagcaccacc ggcttcatta tgaagaacag gtgtttccac ttcaaggaat
     1321 cctttttggt caaggtaacg acggatttca gagatgattt ttgaacgagt gacaaagcgt
     1381 tcaaagcttt cacgattaga aatcaagtca aggtaacgtt tacggtaaat tgtttcaacg
     1441 tctgtcaaac catggaattt ctcaggaaga ggacgaagag ccttagacaa gtgtgtgatg
     1501 tgggttgcct tgatagagag ttctcccata tccgtacgca tcacttcacc ttcgacacca
     1561 aggaagtcac caaggtctgc ttttttgaag atttcgtagt tttcttcacc gacagcatcc
     1621 ttacgaacgt agatctgaat ctggccttcg cggtcttgaa ggtgggcaaa accaactttt
     1681 cctttaccac gtttggttat caagcgtcct gcgatagtag ctgtttcgtt tttatcgtgt
     1741 aattgttctt tatcgaggtt ggcatattta tcttttaatt cttgtgaatt tgcagtacgt
     1801 tcaaaacgtt ttccgaaagg atcgattcct tgttcgcgga gcgcagccat tttttcacgg
     1861 cgaacgatct gctggtcatt tagttcttcc atatgttctg tagacatggg atcctcctag
     1921 ttttactcaa aattgaatac gatgagtcat tttttgcgac actcccattt tatcataaat
     1981 aagcaagaaa ttcacggata atctttaaaa actaaaaaga acctgacgct aaaatcactg
     2041 gaaagaagtg aaagtatctc ccgccccact agcgatatag ccaaatgctg ctttaggaat
     2101 aacttgttgc gccattggct ccaaatcata ggtattgatg aaatctacat gaccttctgc
     2161 attgcttgtt ttgtatgaca taaaatgtcc tccttaataa gtaagcgttt actttgtgta
     2221 ttacaaaaat atcttaactc tttttcaaaa cttttaaaat attttgtttg gaaatttcag
     2281 aaattttatg tctatgataa aaatccttat aacggcaata aaaaatagat attatccaaa
     2341 gaagatttta agtgctacaa taactgtatt atttctagat gggaggttct atttttggat
     2401 tgatccattg ttgaacaata tctaccacta tatcaaaagg cattctttct gaccttgcat
     2461 attgcagttt ggggaatttt gggatccttt ctgctcggtt taatcgttag tatcatccga
     2521 cattatcgaa tccttgtttt ggcgcaagta gcgacagcct acattgaatt gtcacgtaat
     2581 acgccccttt tgattcaact cttctttctc tacttcggtc ttccccgaat cgggattgtc
     2641 ctatcttcag aagtctgtgc aacgcttggg cttgtctttt taggaggctc ctatatggca
     2701 gaatctttcc gaagtgggct ggaagccatc agtcaaaccc agcaggagat tggcctcgct
     2761 attggtctga cacctctaca ggtcttttac tatgtggttc ttccgcaagc aacagcggtg
     2821 gcactcccct cctttagtgc caatgtcatt ttccttatca aggaaacctc tgttttctca
     2881 gcagtggctt tggccgacct catgtacgtc gccaaggatt tgattggtct ctactatgag
     2941 acagacattg cgctagctat gttggtagtt gcttatctaa tcatgctgct acccatctca
     3001 ctggtcttta gctggataga aaggaggctc cgccatgcag gattcgggaa tccaagtact
     3061 ctttcaagga aataatctcc tgagaatctt acagggattg ggcgttacga ttgggatatc
     3121 catcctgtct gtcctcttat ccatgatgtt cagaacagtc atgggaatca tcatgacctc
     3181 ccattctaga atcatacgat ttttaacacg attgtatctg gaatttatcc gtatcatgcc
     3241 ccagctggtg ctactcttca tcgtttactt tggcttggct cgaaacttta atatcaatat
     3301 ctcaggtgag acttcagcta ttatcgtttt taccctctgg ggaacagctg aaatgggaga
     3361 cttggtacgt ggagctatca cttctctccc taaacatcag tttgaaagtg gacaggcact
     3421 cggcttgact aatgttcaac tttactacca catcatcatc ccacaagtct taagaagact
     3481 gctaccgcag gctatcaatc ttgtcactcg gatgattaaa accacttcat tagttgtttt
     3541 gattggggtt gtggaagtga ccaaagttgg acaacaaatc atcgatagca atcgcctgac
     3601 catcccaact gcttcatttt ggatttatgg aaccattcta atcttatatt tcgcagtttg
     3661 ctaccctatt tccaaactat ccactcactt agaaaaacat tggagaaact aaatgtctga
     3721 aactatctta gaaatcaagg aactaaaaaa atccttcgga gacaatccca tcctccaagg
     3781 actttctcta gaaatcaaaa aaggggaagt tgttgtcatc ctagggccat ctggttgtgg
     3841 gaaaagtacc ctccttcgtt gcctcaacgg cttagaaagt attcaaggtg gagatattct
     3901 tctggatggt cagtctatcg ttgaaaataa aaaagatttt cacctagttc gccaaaagat
     3961 tggcatggtc tttcaaagtt atgaactctt tccccatctg gatgtcttac aaaacctcat
     4021 cctaggccct atcaaagctc aaggaaggga caagaaagaa gtaacggaag aagctttgca
     4081 attactagag cgtgtcggtt tgctggataa acaacatagc tttgcccgtc aattatctgg
     4141 tggacagaag caacgtgttg caattgtccg tgccctccta atgcatccag aaatcatcct
     4201 ttttgacgag gtgactgctt cgctggatcc agaaatggtg cgtgaggtgc tggaacttat
     4261 caatgatttg gcccaagaag gccgtaccat gattttagta acccacgaaa tgcagtttgc
     4321 ccaagccatt actgaccgga ttatcttcct cgaccaaggg aaaatcgctg aagaaggaac
     4381 agctcaagcc ttctttacca atccgcaaac caaacgagcc caggaatttt taaacgtctt
     4441 tgactttagc caattcggct catatctata aaggagattc ttatgaaact attcaaacca
     4501 ctcttaactg ttttagcact tgcctttgcc cttatcttta tcactgcttg tagctcaggt
     4561 ggaaacgctg gttcatcctc tggaaaaaca actgccaaag ctcgcactat cgatgaaatc
     4621 aaaaaaagcg gtgaactgcg aatcgccgtg tttggagata aaaaaccgtt tggctacgtt
     4681 gacaatgatg gttcttacca aggctacgct acgatattga actagggaac caactagctc
     4741 aagaccttgg tgtcaaggtt aaatacattt cagtcgatgc tgccaaccgt gcggaatact
     4801 tgatttcaaa caaggtagat attactcttg ctaactttac agtaactgac gaacgtaaga
     4861 aacaagttga ttttgccctt ccatatatga aagtttctct gggtgtcgta tcacctaaga
     4921 ctggtctcat tacagacgtc aaacaacttg aaggtaaaac cttaattgtc acaaaaggaa
     4981 cgactgctga gacttatttt gaaaagaatc atccagaaat caaactccaa aaatacgacc
     5041 aatacagtga ctcttaccaa gctcttcttg acggacgtgg agatgccttt tcaactgaca
     5101 atacggaagt tctagcttgg gcgcttgaaa ataaaggatt tgaagtagga attacttccc
     5161 tcggtgatcc cgataccatt gcggcagcag ttcaaaaagg caaccaagaa ttgctagact
     5221 tcatcaataa agatattgaa aaattaggca aggaaaactt cttccacaag gcctatgaaa
     5281 agacacttca cccaacctac ggtgacgctg ctaaagcaga tgacctggtt gttgaaggtg
     5341 gaaaagttga ttagtcatta actcttaaaa ggaactggat tttaagctcc aatccctttt
     5401 taagatttta cctataacat cctgagtcta tctaagatgt tcaatctgaa cacagtgtac
     5461 atactttatc ttctattgca tatactttat cacataagat acgaatatcc tcttcactat
     5521 gactagcaat caaaattgtt gtcccttttt cactagagag ctttctaaac aatgttctca
     5581 tattttctac acttgattta tccaaggcat tcataggttc atctagtaaa agaatagagg
     5641 gattctccat aattgcttga gcaatcccta gctttttcct catacctagc gaataagttt
     5701 taactttctg gtctttttgc tcatatagac caactatttt cagtgtatca ttgatttcct
     5761 gattaccaac tactcctcgt atgcttgcca aatattgtaa attcttaaag ccactataat
     5821 aatttataaa accaggttct tcaatcaaag ctcccaaatt agctggaatt tttctctcag
     5881 gaacaatatt ttccccattg attaacactt ctccataaga cggactatat aaaccagcta
     5941 ttaatttaaa caatacactt ttccctgagc cattcgcacc agtaattcct ataatttccc
     6001 cctgtttaca actaaagtta aggttttgaa aaacacatgt cttttttaat ttcaactcaa
     6061 tattttttaa tgtaattatt tcattcattc tataaacctc ctcttttgac gagtgaaata
     6121 gaaaatgctt tgaaaaagaa agactaaaaa tagcaactga agaaataaat ctcgtcctat
     6181 atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc ctacaaatag
     6241 accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca tttgtacatt
     6301 cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat tcaaagaatc
     6361 aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt gaatcactag
     6421 aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct gatacctaat
     6481 ccagagctta atagaagaaa atcgaatcac ttccataaag aaatcggatg ctcttctacg
     6541 atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc atatagataa
     6601 gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa caagtctatc
     6661 atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa gaacataacc
     6721 ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac ttaactttcc
     6781 catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag tcaatggaat
     6841 tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat ttaccatata
     6901 ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc agaataagga
     6961 ttctacaaat aaaaaagtaa acagaaatac taagctcatg tttgattttt taaaccaaga
     7021 aaaactgcta atagtaagta aggataaaaa gaaatagtat gctatataag agaaaaaaat
     7081 cctataaaga aactagcatt gtttgcaata cttataccat aaaattctct taaaaaatca
     7141 acctccttta tctccaaaga gaagctaaaa ccattactaa atgcaattag aaaaatcaat
     7201 aaaaataaag tcgccgtcca aatccccgta ctaagagctg ctaatttgaa actaaaactg
     7261 gtaaagtgct taattgattt cagacgaatg cgacactcca acctattaaa atagttattc
     7321 atcaaataaa aaaagaacaa tatatatgtg aacggaaagc aatatactcc agtcgtcata
     7381 tcttgaagta aaactaagat ccattctaat acatttggat ggattgaata ttggcgacag
     7441 cgcaataaat atactgtact agataaaaca caggatagca gtaatataaa ataaaccaat
     7501 actgctaaga aatctttttt ataaaattga acaaattgtt tcattataca tagtcctctg
     7561 aatgtagaaa aaatgtacca taaacaacca aacaactaac aaataaaata aaagcaagat
     7621 gagcactaac taaggaaaga ctgatatctt tctgatatcc caaagctagc gttgtcacag
     7681 gttctaagta agatagccct aaaatagccc aaaaaatacc accaaccatc atataggcaa
     7741 ctgggatgaa aatagctcct atttttttct tcactagcaa agcactagct agtccaaaaa
     7801 tagagaacac agcgccccaa actccatacc agagagtcgt tacaagacta tagagaaact
     7861 gattggaatc aaataattct tttaaggcac cactataatc tccaatataa atttcctgat
     7921 aaggagtcac taaaagatta attcctaata ataataaata ggggagaaaa aagactagaa
     7981 aagaagaaat aattacagta ctacccacaa tagccagata cttcttttta gaaattcgac
     8041 acaattgtgc tgttagaaaa tgactctcag catcctctat tatctgacta gaatagggca
     8101 gtgtacagat aagtgcagct actaggctaa tcggtgaaaa tcctcgaata gaagaagcgg
     8161 caaaaaaaat cgataaacct tcaattttat aaataccatt gaaagcaagg aaatttccta
     8221 aactcatgca aagaagggtc aaaaataaag acatataaaa tcgaggtgat tgaacgactc
     8281 gtacaagatt acaaatgaaa aattccatcc ttactcctcc ttataataaa aatagggtgt
     8341 agcattcttt tttcatgcta cacccacaat caaccatctt taaggcttac tctgacaagt
     8401 aagttaataa gaatctggat tccaagaacc tgaagtatga attcttacat gatttccaaa
     8461 ttgtggcgcc atagctaatc tagtaccaga accaatataa ttgtcaccac ctccattata
     8521 gtacatgaca atcctagagc cagaccccaa tgaatatacc ggggtaatat ctgacccact
     8581 ataggcgcta cgaatagagg tatttaacct tttaccgcca ccagtgctgt cattattatt
     8641 aattccagca gaggagtttt ctttctcaag ctcactagtt gtatccgaac cccaacgcgg
     8701 cgacgttaaa tcataatctc caatctgtct agctaaaact gtagggagaa acaaactaga
     8761 tgcatttgta actacgcaaa aaaggaatat atttttcttc ttttcatgac atcgtcctct
     8821 taagaagtga taaacaagat agttgattga tcaatttgat acatattaca ttttcattat
     8881 atcaacaaaa gaaagcgttg tcaattattt tatttaaaat attgtcatat tttaaataaa
     8941 atggtaaaag agactcggta gcctcgtctc tctttatctt actgacctaa ccttcttgcc
     9001 aattttcttg gtcttcttta aagcgtttta gaagatcaag gccctctggc gtgatgtaac
     9061 cttcttcttg ggctagatgg ataagctcgc tatagtttga aagcgtcaca agtttaacac
     9121 cagcatctgc aaagttctta tctgcttttg gcaattggta gctgaaaatc gctacaactc
     9181 caagtacatc tgctccttct cgcttggctg ctgctacagc ttcaagaact gaaccacccg
     9241 ttgaaataag gtcttcaacc actaccattt tttgaccttg agctacgcga ccttcgattt
     9301 gattaccagc tccgtggtct tttggtttac tacggatgta ggcaaaaggc aagtccatct
     9361 tatcagcaat aatggctccg tgtggaatcc ctgctgttgc agttcctgca atcacttcta
     9421 cttcaggaaa ggcttctttg atagcttcca caaaaccatt ttcaattagg gtacgagttt
     9481 ctggataggc tagtgtcaca cgattatcag tgtaaatcgg tgacttgata ccagatgccc
     9541 aagtgaaggg ttcctctggt ttgaggtaaa cggcttggat tttcaagagg tggctagcga
     9601 tatctttagc aagtgtcatg gtattctcct tttatttttc taatctagtt ctttaattcc
     9661 agtcctgtgt ccattcatcc ttgatggcat gataagctgc aacaggatcc tcagcttggg
     9721 taatgggacg tcccactacg atatagtcac tgccgatttg ataggcatca gcaggtgtca
     9781 tgactcgttt ttgatctcca actgcaacac cagctggacg aatccctggt gtcagacaga
     9841 taaaatctgg attggtagcc tgcttgatga cttgtacttc ctgagccgag caaacaacac
     9901 catccaagcc agcttcagct gtcttcttgg catagtgaat cacagactct tgcagactgg
     9961 tttggatatt ttgaaactcc tgcatctgag cttctgacgt tgatgtgagc tgagttacag
    10021 cgatcaattt ggcttgactc ccaagacctt cacgcgccgc cttcatcatc tctacaccac
    10081 cagccgcatg gacattagtc atatcgacac caagctgaga caagatcttc atggctgact
    10141 tgactgtatt aggaatgtca tgaagtttga gatccaaaaa gacactatga cccaaacctt
    10201 ttaagtagga cacaatctca ggccccgctg cgtaataaag ctccatccct accttgagat
    10261 aaaggctttc ttctgctggg aaaagagcta aaaattcctt gaccgcctca aaactaggaa
    10321 aatcaagagc aatgattgga cgatgttctc gcataggttt ctccttttac ctttactagt
    10381 gagagagtct ggccaacaga agccacgaaa aaaggaacct gccaacagca aggttccacg
    10441 aaaaatgggt agtctccacc ctttcaccgt ctaaccttag ctgcctctct ggactgcttt
    10501 aaaggttttt tactattcta ttatttctac tagcacttgt caagtaaaaa cagctaaatc
    10561 catgttgact gctattgaac acctcacaag actctttcca aaagaatagt taaagacttt
    10621 cacttcggac agagaaaaag agtttgcttg ttacccttta attgaacaaa tgggaattct
    10681 gttttatttt gcggatgcct attcatcttg gcaaagggga agtaatgaga atgcgaatgg
    10741 acttcttcgt gagtattttc ttaaaccgat ttggcaacaa ttaccgaaaa aatccttaat
    10801 gatgctttgc tagcttatta atcaccgtcc ccaaaaatac ttggattaca agacaggttt
    10861 tgaagcactt tttgaagcac ttatagagga gatttaaaaa tgttgcattt tttattgcaa
    10921 tttatcagtt atcttatcac ccaaaacttc tgcaattagt atgagcacta ttgttatttt
    10981 tcttttttcg tacactcaaa tcaaaatata gcaataagac tattctgtag aattgctgtc
    11041 aaaaagatca gttcattttt tccttcccac ttgtgtttat aatcattaaa ataactcaat
    11101 taagaaattt tctttttcct aatagttatc gtaattaaag ttaatagaag aacaatccaa
    11161 ataagtagtg ttagattagg aattagagtt ttattccatg aaaattcctc ccattttgtg
    11221 aaaaacttag taaatagtcc cataaaacta taatctgtta tcttcgcaac tgtcttatcc
    11281 aaaccagata gcatcggtaa gaaagcaact aaaatcatag caggaagact gataacctga
    11341 gctactactt ggctcttcgc ggttaaacct atcaataaat agaataaaat gatggataaa
    11401 gaggttagaa gaacgattgt aatataatta aaagtatgta caattgtaac tcctaaaata
    11461 agaggagtag ttcccataat cacaaaagtt agcaaaaaag gaagaaacat agttgataaa
    11521 atgtattcgg agcctttaac accactcaac agaagagttt gtaaattgta cttttctttt
    11581 tcttcagaca agataatagt tataggactt ccaacagcca aagaaaaaga aaaaggtaaa
    11641 cacatcatca aaagaactaa tgcctgttga tcgttgacct tcccctgtgt ttccataaga
    11701 tatttataaa aatatgtgaa agcaaaaggc actaaaactt gcaataaaat actcttattg
    11761 gaaaggatga tttgacaacg caaccatacc aaagccagaa accgtttaag catttagctt
    11821 tcctcctgtt aatgtgataa aaatatcttc taaagtaggc tcacatgaat gaattgaaaa
    11881 aatattttcc agatcagtct gagatacctg ttctagtgat gtaaaatcaa aagttatctg
    11941 attcccatta taatctgtaa ccttaatctt tttatcttta ttatatcttt gaatgagttc
    12001 agaaggagct ccttgctcaa ctaatttccc tttatttaat aaggcaacat aatcacataa
    12061 aagagttgct tcattcatat catgagtcgt tagaaaaatc gttgtccctg ctgttttcaa
    12121 ttctaaaatt aactcatgaa ttgttcgaga agttgtggga tctagacctg aggtcggttc
    12181 atccagaaag agtacagcgg ggttgttgat aagagctcgt gctaaaagca ttcgttgcct
    12241 cattccagtg gataattttc ctgctaccat cttgcgacta tcatataatc ctactcgctt
    12301 taacaaatta tcaacacgtg atttactaat attataaaat ttactataaa aaagaagatt
    12361 gttatacaga gacattttct cataaaatcc acttgtatcg ctaaccaatc caattctctt
    12421 taattcaccg cttgttaaat tttgagattt ttgtcccaaa ataatagatt gtcctttatc
    12481 ggcaaggaac tgcccagtca gaatattaat cgttgtggtc tttccagaac cagatggttc
    12541 taaaaatcca aaaatctaac cttctttaac agtgaagctt atattattta aagctatttt
    12601 atcaccaaaa ctttttgata aatggctaac ttcaatcata ctcattgcac ctcctttata
    12661 ttctacattt ttacttaaca tctatttact tatctttatc aaataaacgt cttaaaaaaa
    12721 acaataacat taagcatata accgttagca agattgtttc aatattattg ataatacaat
    12781 acattctcaa gcagtataaa ataattatat atatgcaaat gctcattata cctatttttt
    12841 taaacatact aagcctcctt gctaaataat aaagttatat aataactaag acacactata
    12901 cctaacataa taaatatcca cagcattaaa aatattatca taccaataaa aatatccact
    12961 ggtaactgaa taaatgttct aaaattaaca agcaaaaata aaatctcaaa cgatgaaaaa
    13021 agccatacta tattttccaa tgatgtttga atatttttat tgtagtaagc ataatataga
    13081 gcaaaaataa tacccaagaa caatactatt aattgtgttt ctataacagt gaactgccca
    13141 atagcagata aaaaagctac tcctcctgat aataagcaaa aacaaactga acgaataaat
    13201 gttttcgctc taatattgtt atctttcact ttattatttt tttacaaatt ggacattctc
    13261 cgttttccag aacatatttt tcttgactta gagcatgatt tctcatatta taaacgtaag
    13321 cgttatctat taggatttca ttataacatc ccagaaatat tttaattatt aaattagtca
    13381 ttatccctcc caaaatagca ggcatctctg gtgtagtcgg ccactttgtc ttatcagata
    13441 aagtgtactt atttatatct ttgtttatat tccgacaacc aaagcaagaa ccgatggtag
    13501 ggatatatat attatcaata ataagatagc atccattaaa ccctcctgat atgtaggata
    13561 tcttatgcga tacagcaaat tgattgacaa tttttataat atcaatgggc gtatcaattg
    13621 ctttaacgat aaaatctata ctcccatatt ccgcaacaat tttttctaat tcttctactg
    13681 aagaaacttt aatagaaata ggtactacct gaacatctga attatacttg tgtatttttt
    13741 cagaaagaac attaatcttc tccttaccaa catccgactc tgtataaaga atttgcctat
    13801 ttaaatttga tggttctatt atatcgtaat cagcgacaat aagtttcctc actccataca
    13861 tacttagact tagagatata taacatccta agactcctgc tcctaatact aatactgttt
    13921 tattctgtat tttattatac tcttcattat aaaataacga atagtaacct ttagttctag
    13981 aatatctact atcaatatct atctcatccg aaattaaaat ttgttcattt atagttcaaa
    14041 tgttagttta ctgcctaata cttcttctat ctctaatagt tccttacaac tattaaatag
    14101 atcgttgata aattcttgcg aaatttgagg ttctatagct atttcccgca tactccgacc
    14161 acgtaaatac attatacctt cttttttata agcaaaaaca taactattta cgtagaacat
    14221 atttcgtcct tcctatcata tataatatag aaagcagtat agaaacaacc acccctccta
    14281 tactacgaac aataatattg ggtgtcaaaa actgtaatag ttgactcaaa ggaaaataaa
    14341 taataacaaa tgctattaaa agttgaacta tatattgaaa attcaagtta ttattgttag
    14401 aattcaagta tcttaaaata ataccactat catactgaaa aaaaggaatt gaattaataa
    14461 aaatcatacc taagttaatg ataaaaaata agagtccaag caatttatac gatgtgaaca
    14521 ataaaacaag taaacctgta agtgataaaa tcgaatttga agcaattcct gctaacataa
    14581 ttttcaaaaa ttgtttttta ttttttagga attgcacccc acttaaatct acaaaaaaag
    14641 ccaaattaaa caaaagtagc attattccac aatttgggat ctcaatttga taattattag
    14701 caattattgc atgactaatt tcatgtatcg ttgcaaatat aatgacaaca agataggatt
    14761 ttttcaaaat tcttattaaa tcttttatag tttaattttt cagataatgt tatattaaag
    14821 tcccaagata ttttaaaaat atttcttccc tactaacact aataagtccg atatggtaaa
    14881 ttcttgacct attagttgct ctaaccattt ttttcctgtt tcccaactaa tacatgtttc
    14941 ttactggatg gtatatataa atgatagtgt ccctcataag gagtaggtaa tatcttaatc
    15001 ttatcaacaa ttctcataac aaacctaacc tttaataaat aaaagagggg ttaagtaaaa
    15061 catactctag tccctcagtc cgcaatcaat aaaataatga ctaattctat taatctcttg
    15121 ctgcaagact aatacggaaa atttaaatga ctgtctaaaa tgaaattgaa aaagcttgat
    15181 gttagtaaat gaacaaactt caacaaccag caagagcaag gattgctaaa ccaaatccga
    15241 tacctgttgc aagtccagct gaaaaatcat taactggtac tgaaatttct tcaatttcct
    15301 caaataaaaa gttatcttgc atgattatca ctccctatct ttttaacaac aggctactcc
    15361 aattaagaga actaccccag ttccaagtaa aacttccccc aaaaattcac catctgtagg
    15421 ggctacgatt tcttcaattt cttcaaataa gaatacttca tctgacatat gtgttacctt
    15481 cttcatcaaa aattattttg taatcgatta cattgcagat gtaacataaa gaaaaacaga
    15541 tgtcaaatat taaacgtaaa aacatggtca ctaaagaact ataagagaaa aggtaaacct
    15601 agcgacgcga tgaacgctgg gtcgtttggt ttcgattgct ctcttcctct tgttttttct
    15661 gttcttcttc ttgttttttc tcagcttcct tggcctcttg tttggctttt tcctcagctt
    15721 ccataattaa tttatccgcc acagtgtagc tgtagattcc agcttccatg tcgaccacac
    15781 tcggttctga caattgaggc ttaatcttac tgtaatatgg cagtttctta ctcatttcag
    15841 atagaggaac caagacttcg tccgaatcat tcatggtcaa tcgaattaaa tcggatgtca
    15901 ccttgcttgg ggctaattcc accttttgga tagccgcctt gagttctggg ctaatttgag
    15961 caagttctga gacaaaaact ttgatttgtt cactatcatt aaagagaact gataaataag
    16021 tttctggtaa actgttcaga ctcacagaac tagtctcaag ctgaccactg gaaagaatag
    16081 gataatgatt ttcaccagaa atatagtagg ccacaatatc atattccttg accttaatag
    16141 tgaacttagt tggaaattga tagacaagtt gagctgattc aacccaatag ttagacttaa
    16201 tctgcttttc atattttgcc ttgtctagca gaaggttaat cgtataatcc gaatcctgaa
    16261 tgcctgaagc ctgtcgaata tcatcagctg tagtttgcac cgttccctca acacgaatat
    16321 ctttcatggt cgcataagga ctgagcaagt aggcagagac aaacaataga agcagacttg
    16381 gaaataaaat cgtgaaggct cgcaagatat ggataccagg aatctttgct ttggctggtt
    16441 tttcctttgt agccttttta gcaagctttt tatcctgttc ctccttctct ttagactctg
    16501 gttcttcttt ctcttctttc tctttgtcag cctctgagga tgctactttt tcttcagact
    16561 cttccttagc tgattctgaa tcttcctggt ctgtttcact ctcctggtcc tgtttatcct
    16621 ctgacttctc agattcttct cccattcgag cttgtctttc cttttccttc tcctcagcta
    16681 gagccgcctc ttcttcagcc ttctttttta gatattcttg gtttcgtttc tgccattctg
    16741 ataactcttt caattcttcg agggtttctt tgtcctcatt tttcttatct tttgacattt
    16801 actttcctta tgataaatct tttttcaaca attgataaaa atctgctaga gatttcaatt
    16861 ccttagaagc tttcatctta gcttggtaat cttccttgtg acttagtaag tgagaaagct
    16921 tctcttccaa actatccaag gtcaaatcgc tttcttgaag gtcttctgca tagcctttct
    16981 taacaaagta agctgcattt tcaatctggt caccacgact agcttcacga ccaagcggca
    17041 caatgacatg caattttgct atcgccaaga gctcaaaaat cgtattggca ccacctcgtg
    17101 tcacaacaat atcagccaat tccatcaagg gttgatagag atcggtcaca tagtcaacac
    17161 gaaaaagatt ttgcctcaac tcattcagac tagaatctcc agttagattg ataatattgt
    17221 agcgctctgt tagttctttc ttatggtctg tcaccaattg gttaaagaca cgagcgcctg
    17281 cagaaccgcc aacaaacaat acagttggca atttgggatt aaagtgggtt tgaatatcca
    17341 ccaattcatc tggttctgga gtgtttttgt ccgaaacctt ggtcaccgct cccacatgct
    17401 caaccttagc caaactcgaa gcttgttcaa aggttgaata catcttagtc gcaaatttat
    17461 aggcgatttt attggccaag cccatagaca ggtcagattc gtgaataaag acaggcactc
    17521 ctgacacacg cgcagcgata acaggcggta ctgagacaaa gccccccttt gaaaaaaggg
    17581 tctgtggacg cagtcgcaac atgataaaga gcgattggac aattccccaa ccaactttga
    17641 agacgtccag catattttgc caagagaaat agcgacgcaa ttttccagtc gcaatagaat
    17701 ggaaggtgac atccaaacct gacttaagga tttcttggtg ttcgatacca cacttgtccc
    17761 cgatatagtg gacttcccaa ccatcttcga tgaacttggg cattaacaaa agattgaggg
    17821 taacgtgtcc aaccgtcccc ccacctgtaa agacaatttt tttcatatta ttcttttaac
    17881 tccgctactg tgtcgataaa gaggtcgcca cgtacttcaa agttagcata catatcccag
    17941 ctagcattgg caggactaag aagaaccaca tctccttgag tcgcaagctc ataggccttg
    18001 cgggtcgcat ctgcaatatc tgtcgcctcc acataagcga caccagcctt gtctgctgcc
    18061 cgtttgacac gttctgcaga ttgacccagg atgaccatct tcttgagtcc agtaatgtct
    18121 ggcaccaatt cgtcaaactc attgccacgg tccaaaccac ctgcaatcaa gacgaccttg
    18181 ctgttgtcaa atcctgacaa ggctttttga gtagccaaga tattagttga tttactgtcg
    18241 ttatagaatt taacaccctt gatgtcatcc acaaactgga gacggtgttt gacaccaccg
    18301 aaggctgaaa gagtttcctt gatggtttga ttgtccacat cacgaagctt ggctacagca
    18361 atagtcgcaa gggcattttc cacattgtgg ctacctggaa caccgatttc attcgctgcc
    18421 atgactactt caccacggaa gtagagttga ccatcttcca gataagctcc atcaaccttt
    18481 tcaagtgttg aaaatggtac aacagtggct tctgtcttgg aagtcaagtc ttttgccaag
    18541 tcttgattaa agttcaagac aaggaaatca gctgctgtca tcttgttctg gatattccac
    18601 ttggctgcta catattccga aaatgaccca tggtagtcga tatgagttgg catgaggttg
    18661 gtaataaccg caatctctgg atggaattct tgaacaccca tgagttggaa agaagaaagt
    18721 tccataacaa gcgtgtcctt atctgatgct atttgagcaa cctgactagc tggatagccg
    18781 atattccctg ataaaagacc atgttggcca gcagcagtca aaacttcccc aatcatagtc
    18841 gttgtggttg tcttaccgtt cgatcctgtg ataccaataa tcggtgcttc tgaaatcaaa
    18901 taagccaatt ccacctcagt caagactgga attcccttgg ccaaagcctt ttcaatcatg
    18961 ggattgttgt aggggatacc tggatttttc accataaggg caaactcttc atccaagagt
    19021 tccaaaggat ggccacctgt aatgaccttg atcccttctt ccagcaaact ttgggcagct
    19081 ggattgtcct cgaaaggttt cccatcattt actgtcacaa tggcacctag cttgtccaac
    19141 aaacgagctg cagattcacc agacttggcc aaacctaaaa caaggacttt cttattttta
    19201 aattgatcta ttactttcat gtctcgaact ccatttctac tcctactatt ttaccatttt
    19261 tatggaaata aaaaagccac aaagtgtgtt tgtgactctt tcttctaact gaatcttacc
    19321 atatcatcta tgtgataaat cggtaactcg aatgacctga tccacttgct cccaaatcag
    19381 aggattatgg gtcgcaataa taatggtccg attcggattt tttaaagatt ctaggatgga
    19441 aagtaattcc tcagagtttt tggggtctaa ggaagcggtt ggttcatctg cgaggatcaa
    19501 aggtggatcc tttaaaatta tcttcgctag tgcaacacgt tgtgcttctc ctcctgataa
    19561 ctcaaatata ggttgcttca aatccaaata agagaggttt acacggttta gagcttgttt
    19621 catcaaagag attttctctt tttccttcaa ctttttacca actaaaccca gattgagatt
    19681 ctctttgacg gtttggcttt caattaagcc aaaatcttga aataagtatc ctaagtaatc
    19741 tctaaagaaa acagaaggct tgatgtcctt aagagaagtg ccatcataga tgatttgccc
    19801 tttgtcatat ggctccaatc gtccaatcat attcaagagt gttgtcttac cacagccact
    19861 tgtaccgatt aaggcataaa ttttcccacc ttcaaaatga agattcatat ctgaaaatag
    19921 ctgacggctt ccaaattttt tagatatatt ctttagttca atcatcctat tttcctttca
    19981 taattgtcat agaaacacga gattctttct gcgcttgacg gtaaagcgtc aaaactgcac
    20041 tagctagaaa gaccaataaa gtgagcaagc caatcaccaa gtctcgactg cttaaaataa
    20101 agagactagc accaaataca aaactagcaa attggctaac catatactga gcatgtgttt
    20161 caaaaaatcg taaacctgaa attcgtttaa tcaagatatc tcggcggaat tgctcgaaat
    20221 atagaagatt gacagaataa aagagtaaca aggaactggc tattccaaca atagctccta
    20281 agattaaagt tgctgtttca gtttgaactt cattataacg agttagataa acacttcttc
    20341 cttctttaag ataggatact tgctcataaa ttccagcttt cttcaagagt tctagcccac
    20401 tctcatatcc tttgataaag agttgttttc cagcattgat agaccaacta gataaggata
    20461 taaaactatc acctgtagaa gtcggcgtga ataccactaa aatcggatca gtcaaatact
    20521 gagtagatac gggattctca ccgttattat aaacaaaccg cttttctccc attgaaagat
    20581 aactaacgtg cgctttcatc tcataatcca aaggagcact tgcctcctca ccagattttc
    20641 cataataact caatctttct tcaaaaactt tcttaagttc tgcttctcga gagcgcaaat
    20701 gttctgggag caagaggata aactcacctt tttggagatg ggctaacttc tgtttggtct
    20761 cagcatctac cacgaccttt tccttgtcca aataactggg actaacatag agcgtattag
    20821 catctggact ataggtatcc agtgtctctc cctgttcatt ttttccttgt ggattggcaa
    20881 aatggagcag attatccttt acataaagag cttgttcttc ttcgattgct tccttggcaa
    20941 aggcatacca cttgctctga ttttctgtat cttttcctct atcacctaag ccaaaggaaa
    21001 tctggtaata gtctgctctg tcctgccatg cttgttttga aatttcaagt tctttcaatc
    21061 gttggtaaga cgtcaaacct gtcttaacag cgtagcctac tgtaaaaaca gctactaact
    21121 gacacaatag ggttaaagcc atcaagcgtt taaggggtaa tcttccctta ataacgggaa
    21181 ctaatgcttt gtaactcaaa ctcattaggt aaaggagcat tagtaaaatt gaaatcgcca
    21241 ataaaaacaa cagatagaaa ctaatcccaa aaccataggt ggctaacaag ataggataaa
    21301 acaaaccttg actaaaaaga acgactcccc cacctaggaa ggaaaggagg gctgatagaa
    21361 ggagccattt gatatcagta gataaagaat gccccatgat ggataagaga gtctgaccag
    21421 aaaagagttt tatacctgct gctctcattt ccttaatccg agtgataatc actaaagcaa
    21481 agaaagataa gccaaatatt gctaaactaa ttaaaataag gggatttagt aatattcgaa
    21541 aagcaagaaa atagggcggt atctttcggt cagcacttgc tttataaccc aaatctccta
    21601 atttatcggc aagcttttct ttcgtcaagg agcctgacaa aaggagataa ctatttagcg
    21661 gatcactacg ttcacgactt tcttggctag cttcttggaa ttcttttggt aaagttccct
    21721 gaccataagt tgcataagta aagtgagtcg tcccatcctt actcggctct acaattcttc
    21781 tagctattaa actctgttct gagtttgcaa aattctccaa ttcctgttca aatacctcac
    21841 gcgtcggttc ctgagtatct tttttgacac gaagtaaaga aacggaatca tagcttgcat
    21901 ataaatattg tggcgcacgt aagacaataa tccaagcaag gaagaagctg agaaaaaaag
    21961 ttgataataa tatgaatagt ttcttcatag tagactcctt gtaaacaaaa ttccccctgt
    22021 aatttcttac aaggggaacg atttaaatca atgaacgatt agtcataatc acagtaaaat
    22081 gctacttgtt ctccccattt agtccaaatc catgcatagg aggtttttcc agcaggagct
    22141 tctcctttat cagaatgacc atcccatctg ctcacgatag atgaataatg atatttttta
    22201 ccatgatagt aatttgaaaa agcctaacca cctcctgaac cttctccata tgtccatact
    22261 cctccatctg gatattatac agcagctgat gcagctccca ataatgtaaa acttgaaata
    22321 agagctagag caagtaatct atgttttttc gttttcattt tattttttct ttcaaaaaaa
    22381 gcacaccttg agcaacaatg caacaaaata aatcctcctc tctcttttat cgaaaccgct
    22441 ttcttatgtg ataagaataa cttttttatt atttgttgtc aaggaaaaaa tcgaattttt
    22501 tagatatttt actatattac ctctgtgaat aatattatat agtagtttta tttcaaaata
    22561 atatgcaacc agtactaacc aaatataaaa tagatgccat taacgaattt tattcaagtt
    22621 tttcccattc atactataca agtaaaagag atggtgttaa ctaaaaagca attcaaacta
    22681 ttgtaaaatt cctagcaaaa agagagccga aactctcttt tttatcttct tttacttttt
    22741 ttgactggca tgagtgtgat gtctctaaca ctaaagtaag ctaggatcaa catggctatt
    22801 gctaggaata tttctgttgg taattgaaaa attttcagaa aagatagaac caataaaatc
    22861 aagagtgcca ctaaaataca taccatagcg acgatattga cagtcccttt aatgctttct
    22921 ggtgtcgcaa atacatagag taggagcagt aaaattccta ggactaaata gaccatcttt
    22981 ctctctttct agctcttatt cagctgattt tttcttcttg ttagctttct cacgctctgc
    23041 tttgttaagg atttgtttac gcaaacggat agactcaggc gttacttcca tgtactcatc
    23101 gtcgttcaag aactcaagag actcttcaag tgtcaagata cgaggcgtct tgataacagc
    23161 tgtttggtcc ttagtagctg aacgaacgtt ggtcatttgt tttgccttcg tgatgttaac
    23221 tgtcaagtca ttttcacgag agttttcacc gatgatcatt ccttcataaa cctcagtacc
    23281 tgggttgaca aagatcgtac cacgttcttc gatagacatg attgagtaag ttgtagcctt
    23341 accagcatcg atagaaacaa gggcaccacg gtgacgtcca ccaatttccc ctggaatcaa
    23401 tggcaagtat tggtcgaagg tatggttcat gataccgtaa ccacgagtca ttgataagaa
    23461 ctcagttgag tatccaatca aaccacgcgc tggaacaagg aagaccaaac gagtttgacc
    23521 attaccagtt gaaatcatat ccaacatttc acctttacgt tcagaaaggc tttggataac
    23581 agacccttgg tattcttctg gagtgtcgat ttgtacacgt tcaaatggtt cacatttaat
    23641 accgtcgatt tcttttacga taacttctgg acgagatact tgaagttcat agccctcacg
    23701 acgcattgtt tcgataagga ttgacaagtg caattctcca cgtcctgaaa cagtccattt
    23761 atctggtgaa tcagttgggt caacacgaag ggaaacgtct gtttgcaatt ctgcctgcaa
    23821 gcgttcttcc accttacgag aagttaccca tttaccttct ttaccagcaa atggtgagtt
    23881 gttgaccaag aaagtcattt gaagagttgg ctcatcgatg tgtaggattg gaagagcttc
    23941 tactgcatct gtcggagtga tggtttcacc gacaaagatg tcttccatac ctgaaacggc
    24001 aatcaagtca cccgctttgg cttcttggat ttcacgacgt tccaaaccaa agaaaccgaa
    24061 gagttttgta acacggaagt ttttagttgt accgtcaagt ttagaaaggg taacttggtc
    24121 cccaacctta actg
//
LOCUS       DBDESG010000029        24033 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.029, whole genome shotgun sequence.
ACCESSION   DBDESG010000029 DBDESG010000000
VERSION     DBDESG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.029"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(114..254)
                     /locus_tag="ACKB38_001167"
     CDS             complement(114..254)
                     /locus_tag="ACKB38_001167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979937.1"
                     /translation="MKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVDAFM
                     AL"
     gene            complement(256..540)
                     /locus_tag="ACKB38_001168"
     CDS             complement(256..540)
                     /locus_tag="ACKB38_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979938.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(1974..3197)
                     /locus_tag="ACKB38_001169"
     CDS             complement(1974..3197)
                     /locus_tag="ACKB38_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGL7979939.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            3365..4687
                     /locus_tag="ACKB38_001170"
     CDS             3365..4687
                     /locus_tag="ACKB38_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGL7979940.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLSGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            4689..6575
                     /locus_tag="ACKB38_001171"
     CDS             4689..6575
                     /locus_tag="ACKB38_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HGL7979941.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            6743..7087
                     /locus_tag="ACKB38_001172"
     CDS             6743..7087
                     /locus_tag="ACKB38_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGL7979942.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            7097..8509
                     /gene="lacG"
                     /locus_tag="ACKB38_001173"
     CDS             7097..8509
                     /gene="lacG"
                     /locus_tag="ACKB38_001173"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGL7979943.1"
                     /translation="MENLQVKVLPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            8578..10257
                     /locus_tag="ACKB38_001174"
     CDS             8578..10257
                     /locus_tag="ACKB38_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGL7979944.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIIPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGANLTEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(10383..11822)
                     /locus_tag="ACKB38_001175"
     CDS             complement(10383..11822)
                     /locus_tag="ACKB38_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGL7979945.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(11826..13175)
                     /gene="trkA"
                     /locus_tag="ACKB38_001176"
     CDS             complement(11826..13175)
                     /gene="trkA"
                     /locus_tag="ACKB38_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGL7979946.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            13363..14211
                     /locus_tag="ACKB38_001177"
     CDS             13363..14211
                     /locus_tag="ACKB38_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGL7979947.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            14226..14774
                     /locus_tag="ACKB38_001178"
     CDS             14226..14774
                     /locus_tag="ACKB38_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGL7979948.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            14781..15038
                     /locus_tag="ACKB38_001179"
     CDS             14781..15038
                     /locus_tag="ACKB38_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979949.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            15140..16822
                     /locus_tag="ACKB38_001180"
     CDS             15140..16822
                     /locus_tag="ACKB38_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979950.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            16807..17637
                     /locus_tag="ACKB38_001181"
     CDS             16807..17637
                     /locus_tag="ACKB38_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGL7979951.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            17830..18333
                     /locus_tag="ACKB38_001182"
     CDS             17830..18333
                     /locus_tag="ACKB38_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGL7979952.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      18387..18621
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            18786..19349
                     /locus_tag="ACKB38_001183"
     CDS             18786..19349
                     /locus_tag="ACKB38_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGL7979953.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            19339..19989
                     /locus_tag="ACKB38_001184"
     CDS             19339..19989
                     /locus_tag="ACKB38_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGL7979954.1"
                     /translation="MRDKQTFLMKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIR
                     GDLPDYLTILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            20017..20406
                     /locus_tag="ACKB38_001185"
     CDS             20017..20406
                     /locus_tag="ACKB38_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGL7979955.1"
                     /translation="MKVNIADLHLTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            20411..20860
                     /locus_tag="ACKB38_001186"
     CDS             20411..20860
                     /locus_tag="ACKB38_001186"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7979956.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            20988..21575
                     /gene="rpoE"
                     /locus_tag="ACKB38_001187"
     CDS             20988..21575
                     /gene="rpoE"
                     /locus_tag="ACKB38_001187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGL7979957.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            21965..23572
                     /locus_tag="ACKB38_001188"
     CDS             21965..23572
                     /locus_tag="ACKB38_001188"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGL7979958.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         7195 a         4000 c         5193 g         7645 t
ORIGIN      
        1 tgaatctttc taatgagttg ttttgtcgct tttcattata ggtcatatgg gacttttttt
       61 ctacacaaaa ataggctcca taatatccat aggggattta cccactacaa atattataga
      121 gccataaaag catcaacatc ttgtgtggca cgtaaaccat gatattctaa gacataacca
      181 ccgacacaga taattgttaa agtcagattt tcctgaatta attccttatt tagggcttgg
      241 aagaccggtt tcattttatc catgtttgta tttctccatc aatttttgaa cattggttaa
      301 cttagcaggt ttatttaatg aaatcacata tttatgagtt ggagtatgct cttgaaaggc
      361 atacttcaag ctaatttttt gaaaacgggt tagttctttg gagtctagca acttttctac
      421 atcttggttt gttaaataag aatagtcttc aaaaataact tttcgcaaat agcgaaacca
      481 atggcttgcg ttgaattgtg attgtttgga tgcctgtatc atataatcgt accaatccat
      541 tgtcttcctc ctataaaatt tagtttatat tattgtagtt tatattcaaa aatgtagttt
      601 ttcttaatta gctcgtatct ataggatgat actgaaaatc ttcttgttgc tttaaatcac
      661 aatcgaaaaa ttctgttttt catcttagaa aagaatgttt agaaatgtaa aatgaacctt
      721 ttttcttatc cgaaaacctt actaaagtat ataatttagg aagtcaatcc tgtatgataa
      781 tgtttgtact atcgtcgagt ttttgcttga ttgcctaacg agataaattt tagaagaatt
      841 ttcggtgtga tttgacaagc ttctgactgg aaggatagtt acactagtat tgtttattaa
      901 aatgagcaga tagagaaaat ttggaatcta tttgcttatt atcaatcgtt tttttagctt
      961 atttgtctta gattggtata gaactgagct atggattaat actcttgtat cttatcctgt
     1021 tcctaaatat gttggtagaa aaatgaagca gttgttacga gtgtctgtat tggaaaaaat
     1081 tttgtctata gaattcttta aatgatatga aggctaggaa tgccaaagca ctgacatatt
     1141 tcgtttgaat ctcatccgct ctctttactt gtatttgtag aaatgctatc agtaatatat
     1201 tcgcgaacac tatgatttac aacagtgtgg tcgtacatct ttgtaacttt attatacaac
     1261 aaccatttac aggtgtaaag ttgagcagtg tctttagtaa atttagggat ttgttttgtc
     1321 atagttttga tacctgtatt cgttgtttca tgagcatttg acgtttcttt tcatgtaggc
     1381 atttcaacaa ttagaatgat atttttccga ggatgaactt atatattgtg actttttaga
     1441 gttatgatgc ctcttgattt gactatcgct atcaatttca tccactttta cagtttcatg
     1501 gttttatgaa ccgaaacatc taggtacagg ttttagcatc aattgattgt ttttgagcca
     1561 tatgaagttg aaacgtggag atgcttttct tgtgataacg ccctattcaa agaggaataa
     1621 ccgtttttgg agagagttca gtactatgtg gcgctaaata ctatatgtaa aacatatatt
     1681 aaacaagatt taactaagga gaattgtaaa aacaaaaatg agaatttcat ataaagttta
     1741 ttctaggagt atgtatcttg aataaaaatt gttatttaag ttctgggggt tatgatgaat
     1801 gtttttataa taataaaatg cagctagaag aaaagttatt ttaatataaa tcactcaaca
     1861 tagcgagaag gcggtttcct atatggaagt cgtcttgttt tcatattgtc taaatacgag
     1921 atatctacta caggttatag ttgatttaaa caggtctata gatttctaat atattaaggt
     1981 gaaataatat cttgtaatac attgctatgt tctatgaaaa aagcgacgat agctaatgca
     2041 caagctccta tacttccacg atgtttgttg taaggtaaaa tttcaatttc tatattacga
     2101 gtaaagggaa cgaagtggag ctggtcttgg attttatccc tgacttcatg agtgaaaggt
     2161 tgataattaa gcaattgact gttgatatag attttatcag caggattgat gaggaggaga
     2221 tttgcaatag aagtggctaa taaattgact cctttatcaa tttgttgacg taaagcggag
     2281 tcgcctaaat tataagccgt taaaagggtg tctaaatgaa tgtctttttc agtctttaca
     2341 aggcttttta gtacagttag ttgggaattt ttaaataata attgggcgtg tttgattaac
     2401 caagcatccg aaatatatgt ttgtaaacaa cctttttttc cgcattcaca atattgccca
     2461 ttcaattcga caatggtgtg tccaatttct ccgatataag gatttttaga ggctcctatt
     2521 ttttcttttg tgaaaaagga agtgtaaata cctaatccag cgtgtaggaa aataaagtta
     2581 tcgggggtat tgtgtggatt aaaaaggtat tgtcctatag ccatacaatc gatattattt
     2641 tttacaataa aaggaaaatt gaatctttta attacattta ataaattaaa agattcccat
     2701 atggggttgt ttgtaatgat acttccggag tctttgttat aatgtccagg tattgataag
     2761 ccaaatccaa gtaattttgt ttccaaaggg acgtactcta tagctgtttt tatcatattg
     2821 gtaatggtgg aattgatttc tgaaatagga taatcttcat tcaaaatttc tgtctcgaaa
     2881 agagttttcc cttgtaagtc acatagactg agaaagatac ctaggacgtt tatttcaata
     2941 cctccaacaa catacggaat attatctgtg atagaaatag tttttctacg tcttccagat
     3001 ccggaaaact cgctaaattc atcaccagtt tcgtatatga gggactgttc gcttagctct
     3061 tttatgatgt tagtcgttgt tgctggagta agtcctgttt cattaacaat atctgtacgg
     3121 gatgttgctg gatttcgata tagaaactcc agtatagtgg ctctattttg taaaaaggct
     3181 gattgttttg taatcatttg tttcctcctt tggtttattt tatattaaac ataagaaaaa
     3241 atcaattaaa attattattc tatacaaaga aaaaaatatt gtaaaaacaa caaaaaatat
     3301 attgacaacg ctttctgtta gtggtatatt taatttataa aaaataaaaa aaggagttcc
     3361 ggtgatgaac aatattttag cgtttttaga aacaaaagtc gctccgtttg gtgaaaaagt
     3421 tggcaaccaa cggcatttga aagctattcg tgaaggattt atgatggcaa tgcctttgat
     3481 tttagtcggc tctttatttc ttattctaat cagttggcct caagaggctt ttacaaattg
     3541 gctgaatagt gttggattgc taagtatctt gacaactatg aatcagtcaa cagtagcgat
     3601 tatctccttg gtcgcttgtt tcggtattgc ctacaggttg tcggaaggat atggtacaga
     3661 tggtccgtcg gcagggatca tagccttatc cagttttgta ttgatggcac ctcgtttttc
     3721 gagtatggtt tatgataaaa atggggagca ggtcaagcag ttatttggcg gcgcaatacc
     3781 attttctagc ctgaatgcat cttctttgtt tatggcgatt actattggat tggttacagc
     3841 agagatttat cgtatgttta tccagcgcgg aattacgata aaaatgccaa gtggtgtccc
     3901 agatgtagta agtaaatcat tttcagctct tttatctggt tttactactt ttgttttgtg
     3961 ggctttggtc ttaaaaggtc ttgaagcggc aggagttgca ggaggtctca acggactcct
     4021 aggtgcaatt gttggaacac cgcttaagtt aattgcagga acgcttccag gtatgattct
     4081 atgtgttatt gtaaactcat tcttttggtt ctgtggagtt aatgggggac aagttttaaa
     4141 tgcttttgta gacccagttt ggttacaatt tactacagaa aaccaagaag ctgtggctgc
     4201 aggacaaaca ctccaacaca ttattacatt accgtttaaa gatttatttg tatttattgg
     4261 tggcggtgga gcgactattg gtcttgcgat ttgtctcttc ctatttagta agagtcgtgc
     4321 gaataaaaca ttaggtaagc tagctattat accgtctatt tttaatatca atacagctat
     4381 tctatttacg tttccaacag ttttaaatcc gattatgctg attccgttta ttgctactcc
     4441 tacaatcaat gccttgatta cctatgtatc aatggctgta ggattagtac cctatacaac
     4501 aggtgtaatc cttccgtgga caatgccacc gattatagga ggcttccttg caacaggggc
     4561 tagttggcga ggagctctat tacaagttgt tttgattttg gtttctgtag caatttatta
     4621 tccattcttc aaaattgcag ataaacgcaa tcttgaaaaa gaaaaagcta ctgttggagg
     4681 gaaataagat ggttatcaga gtatttgatc aacagaaaaa tacttattct agctttgcct
     4741 tagaggaatt aagttactat atgaatcggg tctttaagac taacatagag cttgtcgagg
     4801 agaaggaagc ggatattttt gtaggattag tcaataaaga ggacagaaaa gaccatgttc
     4861 ttatctcatt agacaagggt aaggggagaa ttgagtctaa tacaattgta ggtttactta
     4921 ttggaattta ccgaatgttt catgaatttg gggttgtgta tactagacca gggcgcagac
     4981 atgactttgt tccagagtta cgatttgaag attttttaga taaacagcta tctatagatg
     5041 aaacagccag ttactatcat aggggagtat gtatagaggg agcggattca tttgaaaata
     5101 tactagattt cattgattgg ctacctaaga ttgggatgaa cagttttttc atccagtttg
     5161 aaaatcctta ctcttttttg aaacgttggt atgaacatga atttaatcca tatctaaata
     5221 aagaacaatt ttcaaatgaa ttagtacaag aattgagtga taggttggat aaagaattgc
     5281 aaaaaagagg tcttattcat catcgtgttg gtcatggatg gacaggtgaa gttttaggtt
     5341 actcttcaaa atttggctgg gaatcaggtc ttagtatttc agaggagaag aaaccctatg
     5401 tcgctgaaat aaacgggaaa cgagaattgt ttaatacggc tccgatttta accagcctgg
     5461 atttttcaaa tccagatgta gctgataaga tggtagaaat tatcaaggat tatgccaaga
     5521 aaagacctga tgttaactac ttacatgtat ggttgtcgga tgctcgtaat aatatttgtg
     5581 aatgcgaaaa ctgtagacaa gaattggttt cggatcagta tattcgtatt ctcaatcaat
     5641 tggatagggc tttaacgagt gagggattag atacaaagat ttgttttctg ctttatcatg
     5701 agttgttatg ggcacctcag aaagaaaaat tagataatcc tgaacgcttt accatgatgt
     5761 ttgcaccgat tacaagaaca tttgaaatga gttatgcaga tgtagatttt gacaattcca
     5821 tacctacgcc taaaccttat atgcgtaata aaattatact tccgaattct cttgaggaaa
     5881 atttatctta tctttttgag tggcaaaaag catttaaagg agatagtttc gtatatgact
     5941 atcctttagg gcgtgctcat tatggcgatt taggctatat gaaaattagt caaactattt
     6001 acagagatgt atcttatctt tccaacctac atttgaacgg gtacatttcg tgtcaagaat
     6061 tacgtgccgg attccctcat aattttccta attatgtcat gggggaaatg ctctggaaga
     6121 agacaagaag ttatgaagaa ttgattgaag aatacttttc tgctttgtat ggggaaaatt
     6181 ggcagtctgt tgttgaatat ttagaaaaat tatccattta ttcctcttgt gattatttta
     6241 atgcaattgg cagccgtcaa agtgatgttt tagcgaatca ttattatata gcttacaatc
     6301 tagctgataa ttttttacca attattgagg aaaatatttc taagttatta aatagtcaaa
     6361 aggatgaatg gaaacagctc agttatcatc gtgaatatgt tgttaagatg gcgaaggctt
     6421 tatatcttca agcaactgga aaaacaaggc aagctcaaga tgaatggaga aatgtgttga
     6481 attatatccg tgggcacgaa ttgctatttc aatctaattt ggatgtttat cgtgtaattg
     6541 aagtagcaaa aaattacgct ggtttccact tataaatcat aagtatagaa aatgaactaa
     6601 ggtattcaga gaagattgat cctaaatatt atgaaattta aggattttta agatatttag
     6661 ggtcaacttt ctatttatat cgtagcgaag tcattttaat aatgatgtgt aaaagatgga
     6721 tcaagattga ggaggaagaa agatgaaatc aaaagaagaa ataaatatgc ttggttttac
     6781 aattgtcgct tacgcaggag atgcaaggtc agatttgatg gatgctttgg cgtttgcgag
     6841 agatggatat tttgaacagg caagagaatt ggttgagtct gcaaacgact caatagtgtc
     6901 tgcccatcga gaacagacta atttattagc ggaggaggca tatggagata attttgaagt
     6961 gagctttatt atgattcatg gtcaagatac tttgatgaca acgatgctat tgtatgatca
     7021 ggtaaagttt tttattgatg aatatgaacg aattcgaaag attgaagaac atattggttt
     7081 gcaatgagga ttagtcatgg aaaatttaca ggttaaagtc ttaccgaagg agtttttatt
     7141 aggaactgct accgctgctt atcaagtaga gggtgcaact agggtagatg gcaaaggaat
     7201 aaatatgtgg gatgtttatt tgcaagaaaa tagtccgttc ttaccagatc cagctagtga
     7261 tttttattat cgttacgaag aggatatagc tttggcggca gaacatggtt tgcaggcttt
     7321 gcgtttatct atttcttggg ttcgtatatt tcctgatata gatggggatg ctaatgtatt
     7381 agctgttcat tattaccata gagtttttca gtcttgctta aaacataatg tgattccgtt
     7441 tgtttcttta catcattttg attcgcctca gaaaatgtta gaaacagggg attggttgaa
     7501 cagagagaat attgatcgtt tcatacgata tgctcgcttt tgtttccaag aatttacaga
     7561 agtcaagcat tggtttacaa tcaatgaact gatgtctctt gctgcaggtc aatatatagg
     7621 aggtcagttt cctccaaatc atcattttca attatctgaa gcaattcaag cgaatcataa
     7681 tatgttgttg gcgcatgctc ttgcagtcct cgaatttcat caattaggga ttgagggaaa
     7741 ggtaggttgt attcatgctt taaagccagg ctatcctatt gatgggcaaa aagaaaatat
     7801 tttggcagct aaacggtatg atgtttataa taataaattt ctattagatg gaactttttt
     7861 gggctactac agtgaggaca cgctttttca cttgaatcaa atattggaag ctaataattc
     7921 tagctttatt attgaagatg gtgatttaga aattatgaag agagctgcac ctcttaatac
     7981 gatgtttggg atgaattatt atcgttcaga atttattcgt gaatacaaag gtgaaaatag
     8041 acaagaattt aattcaacag gaataaaagg acagtcttct tttaaattaa atgctctagg
     8101 tgaatttgta aaaaaacctg gtattccgac aacagattgg gattggaata tttatcctca
     8161 agggttattt gatatgttgc ttcgtatcaa agaagaatat cctcaacatc cggtcattta
     8221 tttaactgaa aatggtacag cccttaaaga agttaagcca gagggcgaga atgatattat
     8281 tgatgacagt aagagaatcc gttatattga gcaacattta cacaaagttt tagaggctcg
     8341 agatagagga gtcaatattc aaggctattt tatatggtct ttgcaagatc aattttcttg
     8401 ggcgaatggc tacaataagc gatatggtct tttctttgtt gattatgaaa cacagaagag
     8461 atatattaag aaaagtgctc tttgggtaaa agggctaaaa cggaattaag gttagcgatt
     8521 tgactgatgt ttaatatgtt ttaaatatga ggttgaattt tttataggag gagttttatg
     8581 gataagctag tcgctgccat tgaaaagcaa caagggaaat ttgaaaaaat ttctactaat
     8641 aactatatga tggctattaa agatggattc attgctacta tgcctttaat tatgttttca
     8701 agctttttga tgattattat tatgattcct aaaaatttcg gagtagagtt accgagtcca
     8761 gctattgtct ggatgagaaa agtgtatatg ttaaccatgg gagttttggg tattattgtt
     8821 tcagggactg ttggaaagtc attagttgga aatgttaaca gaaaaatgcc tcacggaaag
     8881 gtaataaatg atatttctgc aatgttggca gccatatgta gttatctggt attaactgta
     8941 acgcttgtag ttgatgagaa gacgggatct acaagtttgt cgacaaacta tttaggatct
     9001 caaggattga taacttcgtt tgtcagtgcc tttattactg taaatgttta ccgattctgt
     9061 attaagcgag acattactat tcatttacct aaggaagttc ctggggctat atcacaagct
     9121 tttagagata ttttcccttt ttcttttgtt ttacttatta gtggtttgtt agatattgta
     9181 tctcggttta gtttagatgt tccttttgcc caagtatttc aacaactatt gactcctatt
     9241 tttaaggggg cagaatcata tcctgctatg atgttgattt ggtttatgtg tgctttgctt
     9301 tggtttgttg gaattcatgg accatctatt gtcttacctg ctgttacagc tttgcaactg
     9361 agcaatatgg aagagaatgc tcaacttctt gcaaatgggc agttccctta tcattcttta
     9421 acacctaatt tcgggaatta tatcgctgct attggaggaa cgggggctac ctttgttgta
     9481 ccatttattt tgattttctt tatgcggtct aaacaattaa aatcggtagg taaagctaca
     9541 attactcctg ttttatttgc ggtaaatgaa cctcttctat ttggtatgcc tgttattttg
     9601 aatccctatc tttttgtccc ttttttgatg actccaccag tgaatgtatt tctaggaaag
     9661 gtctttattg atttctttgg aatgaatgga ttttatatcc agttaccttg gacctttcct
     9721 ggtcccttgg gattgttaat tggaacgaat tttcaactta tctcctttgt atttttatct
     9781 ttgattttag ttgtcgacat attgatttat ttgccattct gtagagcgta tgatagacag
     9841 ttactggtga aagaagatat tgcaagctca aatgatatta ttttagagga ggatacaagt
     9901 gaaataattc ctggtgagat agatgaaata aaaagtaagg agttgaaagt actggttctt
     9961 tgtgcagggt ctggaacaag tgcgcaatta gccaatgcaa ttaacgaggg ggctaactta
    10021 acagaggtta gagtgattgc gaattcagga gcgtacggag ctcattatga tattatgggt
    10081 gtttatgatt taattattct ggccccacaa gttcggagtt attatagaga gatgaaggtg
    10141 gatgcagaaa gattaggtat tcagatagtt gctaccagag gaatggaata tattcattta
    10201 acaaagagtc caagtaaagc cttacaattt gtattggagc attaccaagc tgtgtagtaa
    10261 gtttttccat cttttatttg agtaaagatt ttgtttacag ataggcttgg atttaaaaac
    10321 gttccccctt ttttaatata agaatccctc tttcacaatt gtaaaaagag ggattttgta
    10381 ttttatctct tagaccaagt tctcttcata aagagaagga ggattgggta aatctccaag
    10441 cgccctgcaa tcattgcaaa ggataggaga atttttgaga tgggactaaa gattgagaaa
    10501 ctagaagtgg ttcctagaat aggcccgata ttattgaaac agctaaagac agcgctggtc
    10561 acgaccagaa aatcattgct atctaggctg acaataaaga taagcgctag caaaatcata
    10621 gcatagatga caaagtactt gagaatctta tgctgggtat ctttgtcaat caccgtttta
    10681 ttaacatgga gggtcaaaac acggtggggc gataggattg acaaaatttg gtttttggca
    10741 atttttgaaa ggatgaggcc tcgaataatc ttgagtccac ctgcagttga tccagcagag
    10801 ccaccgattg ccatgaggaa aaggaggata aactgggaga agaggggcca gttggtaata
    10861 tctccatatc caaaaccagt tgttgtaatg atgttggaaa cctggaagaa ggtcatttca
    10921 aagctctttg aaaaccctgg gtagaggtag agggtgttga ggctaatcaa gcctgtagaa
    10981 accagtacaa tgaccaagta agccctaagc tcttcatctc caaagaaggc cttgatgcga
    11041 cggagcatga ggtagtagta gaggttgaaa tttactccaa aaaccagaac tccgatactg
    11101 accagatagg taatcagtga gctgccatag tgggcaattc cgtcgttata gacggtaaag
    11161 cctccagttc ccgctgtccc catagcaata acaaaactat cgtagagagg cataccggct
    11221 agataataga tgatgacaaa gagggagaag agagctagat aaaggagata gagaatctgg
    11281 gcagtgtttt ttagtttgga tacaaccttg ccaaaaacag gacctggaac ctcagccttc
    11341 atcacctcta ggtggctatt tttggcattg tccataatag caagtgcaaa aacaagcact
    11401 cccatccctc caatcaagtg ggtaaaactt cgccagaaga ggagggaacg gctgagaacc
    11461 gaaacgtcgt tcaaaatact tgctccagta gttgtaaatc cagaactaat ttcaaaaaag
    11521 gcatcaataa ggctggggat ttgcccagaa aagacaaagg ggagaccacc aaagaaagac
    11581 caaaggatcc aacagagggc aacgatcaag actccctcct tggcataaat ccgttgattt
    11641 tttggcttct gtaaactccc tgaaccgcct aacaatacga gaatccctat ggtcgaaaag
    11701 agggctgtaa agacttggct cgattcacgg taatagacag caatcgcaac aggaaccaaa
    11761 agaagaacag cttcaatcaa aagtaatttt gaaaggaggt aacgaatcat acttttattc
    11821 atttcttacc tcgcgatcaa gtcataaatc ttggtgatgt ttggcaacaa ggttgttact
    11881 aggagcttgt ctccaacttc caacatatcc tccccagttg ggaaaatagt cttgcccttt
    11941 cgaataatgg ctgcaataag aacccctttt ttcaatttca gttgagaaag aggtttggca
    12001 gtcattttat tggcttcctt gatatggaat tgcagggttt cgatttggcc attggctaga
    12061 tggtgcatag cttgaaggtc tgaatactgg gcattaactc gaccacgaat aaagtgcata
    12121 atcgtatcta cagcgatgct tttaggtgtg atgatacttg aaaaatcagg cgcattgata
    12181 atctcgagga gactggtacg attgacctta gtaatatttt tctgtacacc taccctgtca
    12241 aggaacatag atgtaatcag attttcctca tcgactcctg ttagagtcgc aacggcatca
    12301 tagtgttgag cactttcttc cagcaggata tcttttgcgg ttccatctcc ttgaacgatg
    12361 tagagatttg ggaatttctc gctaaagaag ctggcgattt caggattgat ttcaatgact
    12421 tttgtatcga tacgactatc tttgagaata ccaagtagat aataggcaat tctacctgcc
    12481 ccaacgatga gaaggctctt cacggcgcgt gatttaaaat aattatggaa gagtatcata
    12541 tcgacacggt taccagtgac aaagattcta tctttatcct gtacagtcat gtcaccgctt
    12601 ggaatgataa tttgatgatc cctctctatc gcacagacaa tgacattacc aaatttttta
    12661 cgaaaatcag aaatgggcat ttggcaaaga ccgctggtgg acttgacgac aaattccatg
    12721 aggctaacgc gtccaccagc aaagcgttcg acagacaggg cgttggggaa gtcaatgata
    12781 ttcgcgatag cgcgggcagc caagagctca ggattaacga taagagaaaa accgagaata
    12841 ttcttttcct tgaaataaga gttagaatat tcagggttcc gcacccgaac gatagtttct
    12901 ttagctccca ttttcttggc tagaactgct gcaatcatgt tgacttcatc gtgctcagtc
    12961 agggcgataa agatatcaca atcttggacg ctggcttgct caagaatggc aaaatcggcc
    13021 ccgttaccaa ggataccaat gatatcaaag cgactgacaa tatgattgag aacagcttcg
    13081 tcttgctcaa tcagcaaaac atcatgcttt tctgcaacca aggagcgaca gagggcaaaa
    13141 ccaacttttc cccctccgac aaggataatt ttcataataa aacctacttt ttcatgatgt
    13201 aactatcata ccctttttca agaaaaaatg cacctactag ctaataacaa gagtttttag
    13261 tgaaaattcg ctataaggta aaactatacc ctaaccaatt gaaatagcta ttagcgactt
    13321 tctctgaaat atggtatgat aaaggatata caaggagata aaatgaataa taatttactg
    13381 gtattacaat cagactttgg tctggttgat ggtgcggtat cggctatgat tggagtggct
    13441 ttagaagagt ctccaacctt aaaaatacat cacttgacgc acgatatcac gccttataat
    13501 atttttgagg ggagctatcg tctctttcag acggtggatt actggcctga gggaacgacg
    13561 tttgtatcgg ttgtcgatcc aggtgtcggt tcgaaacgta agagtgtagt tgccaagact
    13621 gcaaaaaatc aatacattgt cacgccagat aatgggacgc tttcctttat caagaaacac
    13681 gttggcattg tagccattcg tgagatttct gaggtggcca ataggcgtca aaacacagag
    13741 cattcttata ccttccacgg tcgtgatgtc tatgcctata ctggtgctaa actggccagt
    13801 ggtcacatta cttttgagga agtagggcca gagctcagtg tggaacagat tgtagagctt
    13861 ccagtcgtag cgaccatcat agaagatcat ctggtgaagg gagccattga tattctggat
    13921 gtgcgtttcg gttcgctttg gacctctatc acacgggaag aattttacaa gctggaacca
    13981 gaatttggtg atcgttttga agtgaccatc tatcatgctg atatgctggt ctatcaaaat
    14041 caggttgtct atggcaaatc atttgcagat gtgagaattg ggcaacccat cctttacatc
    14101 aactctctct atcgtttagg tttggctatc aaccaaggtt cctttgccaa ggcctataat
    14161 gtaggtgtcg gttcatcttg gaccattgaa ataaagaaaa tagaaggata aaataggaga
    14221 atagtatgga aatcaaattt acaattaaac aagttgttgc tgtcggaatt ggcgctgccc
    14281 tctttgtcgt catcgggatg atcaacattc caacccctgt tccaaataca agcatccagc
    14341 ttcagtatgc ggtacaagcg ctactttcta ttatttttgg accgattatc ggtttgcttg
    14401 tcgggttgat tggtcatgca atcaaggact ctcttgttgg ttatggtcta tggtggactt
    14461 ggattatcgc tagcggactc tttggtttag ttgtgggact ttttagaaag tacgttcgcg
    14521 tgatcaatgg tgtttttgac tggaaagata ttcttatttt taatctcatt caactacttg
    14581 caaatgccct tgtttggggt gtcttggcac cacttggaga tgttgtgatt tatcaagaag
    14641 cggcagaaaa agtatttgca caagggattg ttgcgggaat tgccaatggt gtatctgtag
    14701 ctattgcagg aactcttctc ttacttgcct atgcaggaac ccaaactcgt gcaggaagtt
    14761 tgaaaaagga ctaatactca atgaaaatca aagagcaaac taggaagcta gccgcaggtt
    14821 gctcaaaaca gtgttttgag attgtggata gaactgacga agtcagctca aaacatggtt
    14881 ttgaggttgt agatgaaact gacgaagtca gtaaccatac ctacggcaag gcgaagctga
    14941 cgtggtttga agagattttc gaagagtata aaatgatggg gaaggctggg caactagtct
    15001 ttttcgatgt ttatagatta gttaggcaag taagctagga taggaatctt tttggcgcta
    15061 agattttaag aggagagact gcaagaaggg cttgatttat gataaaatag aagtctaaga
    15121 agcgaatgaa gagagtaaga tgaaagaagc tataattgag tggaaggatt tctctttccg
    15181 gtatgaaaca caacaagaac cgaccttgca agggatagac ttgaccattt acaagggaga
    15241 gaaagtctta attgttggac catctgggtc aggtaaatct accttgggtc agtgtttgaa
    15301 tgggattatt cccaatattt acaagggtca gacatatgga gaatttttga taaagggtca
    15361 agtagccttt gatatgagca tctatgataa gtctcatctg gttagcacag ttttgcagga
    15421 tacagatggg cagtttattg gcttgtctgt ggcagaagat ttggcgtttg ctctggaaaa
    15481 tgatgtgaca gccctagatg agatgaaagg tcgtgtttat aaatgggctg aaaagctgga
    15541 ccttcttcct ttactggatc agcgtcctca ggatttgtca ggtggacaaa agcagcgagt
    15601 cagtctggct ggtgtcttga ttgatgaaag tccgattctc ttgtttgatg agccactcgc
    15661 caatctagat cccaagtcag gtcaggatat tatcgaattg attgaccaga ttcataagga
    15721 agaggggacg acgactctta ttatcgagca ccgtttggag gacgttctgc atcgccctgt
    15781 ggatcggatt gtcttgataa acgatggtcg tatccttttt aatgggagcc ctgaccagtt
    15841 gcttgcgact gatttattga ctcaaaatgg aattcgagaa cccctttatc taacgactct
    15901 ccgtcaatta ggtgtggact tagtcaagga agaacaatta gcgaatctgg ataacttgtc
    15961 tatctcaaaa ggtcaggttc agttgcagaa tgaactggca aaagaaaccc cagcattgca
    16021 gtcactcttt agactagagg aagtatcttt ttcttatgat gatagaccga ttttaaaatc
    16081 cctacattta gatattaaaa agggtgaaaa gattgctatt gtcggaaaaa atggagcagg
    16141 gaaatcaact ctagccaagg ctataagtag ctttattcag acggaaggac gctatctttg
    16201 ggaaaaacag gatataaaag gcgattctgt tgcagagcgg gcggaacgag taggatatgt
    16261 gctacaaaat cctaatcaaa tgatttcaac caatatgatt tttgatgagg tggctctagg
    16321 gctccgtttg cgaggtgtgg atgagaagga aattgaaacg agagtatatg aaaccttgaa
    16381 aatctgtgga ctttatgaat tccgtaattg gcctatttct gccctgtcat ttggtcagaa
    16441 aaaacgtgtc accattgctt caattttggt cttaggagct gaaattattc tcctagatga
    16501 accgactgca ggtcaagatc agaagaacta tactgagatt atggaatttc tcgaagagtt
    16561 acatcaaaaa gggcatacca ttgtcatgat tacccatgat atgcaattga tgctggatta
    16621 ttcagaccgg gtccttgtca tggtggatgg agaattgatt gccgatactg ttccagccag
    16681 tctgttgagc gatcctgagc tgttagtaaa agccaatcta aaagaaacct ccatctttaa
    16741 cttggctaag aaactagatg tggatccact ggatttaacg gcattttaca aagaaaggag
    16801 agagggatgc aagctaaatt aatcggttac cagcatagag atactgtgat tcatcgcttg
    16861 tcaggagctg ggaaacttct ctttttcatt ctggtatcat tggcggccat gattagctat
    16921 gataccagaa tgctcttgct gattgccatc ttttcagtct ttctcctcta tttatcagaa
    16981 attcgcttta aagatgtttc ctttgtagcc gtttttgcga cgatatttgc cgttttaaac
    17041 gttttgatgg tctatctctt ttctcccgag tatggggttg gactttatgg agagagaagt
    17101 gtgatttggc agggaatcgg tgtctacact ctaaccagcc aggagctctt ttatctgcta
    17161 aatttggcca ttaagtatct ttgcaccatt cctctggcta ttatcttttt gatgacaacc
    17221 catcctagtc agtttgcttc cagtttaaat caaattggtg tgccctataa gattgcttat
    17281 tctgtcagcc tgaccttgcg ctatattcca gatttgcagg aagaattctt tactatcaag
    17341 atgtctcagg aggcgcgtgg gatggaatta tccaagaaag cttctcttat gcaacgaatc
    17401 aaaggcaatc tgctcattat tacgcccttg atttttagct cgctagaacg cattgatacc
    17461 attgcgactg ccatggagct tcgccgcttt gggaaagaga aaaaacgcac atggtatagt
    17521 tatcaggcct tgaaaaaagg agactatatt accttgctct tggcaacctt gtttttagta
    17581 gttagtttac tacttatctt gcagaatcag ggacgatttt acaacccttg gaaatagctt
    17641 gaaaaaattg aaaaaatcaa gtcatttcta ttgacaatga ttctgaaagt gttatactaa
    17701 gaaagtagtt tcgctgattt acttcaaacc tgttgtgagg taagttaacg atgccttaac
    17761 cacgctgttt gctgagcttg actccgggca gtgtggctat ttttttgcaa tggtgaaagg
    17821 aagcaagtca tgacaaatca cattgtatta tttgaacctc aaattccaca aaatacgggc
    17881 aatatcgcgc gtacttgcgc tgcgaccaat tctcccctcc acatcatcaa gcctatgggc
    17941 tttcctattg atgaccgcaa gatgaagcgg gctggattgg attactggga taagctagag
    18001 atttattttt acgagagttt ggaagatttc atatctcaga tgaagggaaa actctatctg
    18061 atttcgaaat tcgcggaaaa agtctattct gaggtggatt tatcgactga cgaggaccat
    18121 tattttctct ttggacgtga agacaagggc ttacctgaag attttatgcg agaacatcct
    18181 gagaaggctc tccgtattcc catgaatgat gaacatgtcc gcagtctcaa tgtgtctaat
    18241 accgtctgca tgattgtcta tgaggctctt cgccagcaga actttgcagg tcttgagctc
    18301 gtccatacct atgaagtgga taaattgaaa taaaaaatga aaatgaacaa aatgcttgcg
    18361 cttgcaagcg ttttttgtta tgataaaaga gtcttcaggg cagggtgaaa ttcccgaccg
    18421 gcggtgactt taactaggaa atgatctttt ccttttatac tttgttgaca agctttgcct
    18481 aaccagaagt tatgcctaca gcttgtcgcc tagtctaaaa gaaaaatctc tatttccttc
    18541 tctttaaaga agtccgtgag cgcaagctga tgtggtgaga ttccacaacc gacagtatag
    18601 tctggatggg agaagacgaa agaatagcct tgtctgtttt gatagattta tagccggatt
    18661 gtaaccactt gctttgtttt tctttaatag agtaagaggg aacttttggg atataaaaag
    18721 tgagaataga tagaggaatc ctttccaact tcttctgatt ttatagaaaa ttggaggaac
    18781 ctgttatgac aaacacacgt cgactttcga ccattgcaat tctatcagcc atctcatttg
    18841 tgctgatgta ctttgacttt ccgcttttac cagcggcatc tttcctcaag atcgaattta
    18901 gtatcttgcc agtccttgtg ggtctggtgg tcatggattt gcctgctgct ctaggagtgc
    18961 tcttgcttcg ctcactcttg aaattgcttc ttaacagcca aggagtgaat acttacattg
    19021 gtttgccgat gaatatcgta gctttgggag tttttgtcat cgtatttgct ttgatttgga
    19081 aaaaggaacg gacaaccctt cgtttcctac taggctctct agctggaact attggtttaa
    19141 ccttggccat gttggttctc aactatgttt atgctgttcc tttgtacgct aagtttgcta
    19201 actttgatat tggaaaaatt ttgggacttt ccaactacct aatgaccatg gtattacctt
    19261 ttaacttgat tgaaggtgtt atcttttccg tttcattctg gttgttgtat gttctcttga
    19321 aaccaacctt aaaacattat gagagataaa caaacatttt taatgaaggg cagttttgcc
    19381 cttttacttt tcgttattct tggctacatg gtcaaatttt accctgaaat gttggtcaat
    19441 tttgaccaat cgattcagac tgccattcga ggagacttac cagattattt gactattctt
    19501 tttcgagccc tcacacgtct gattgatatc ccagtgatta tcacttgggt tgtcattaca
    19561 gcttttgtct tttatcgtaa gcgatggaag atagaaagtt tcttcatgct gggaaatctg
    19621 gctttggcag gtcttttaat cgtgaccttt aaaaatatct accagcgccc acgaccagct
    19681 attttacatc tggtggagga gaagggattt tccttcccaa gtgggcattc tctagctgta
    19741 acgctgatgg tagggacttt gattgttatt cttagtcagc ggattaaaga tcaggtctgg
    19801 agaaaaatcg tacaaatcgt ccttggcctc tacctagtca gtgtgctggt atcaagagtc
    19861 tatctgggag ttcattaccc atcagacgtc cttgccagtc tctgtgtggg cttgggagtc
    19921 ctgtttatcg agtttccctt ttatgacaag ctccgcttcc aatggcgatt taaaggcaaa
    19981 cagaagtgag tatcgaattc ccttgaggag aaagaaatga aagtcaatat agcagatctt
    20041 catctgactc aactatattt atcagaaaag aagttgcaag atattcagat gctttaccag
    20101 tcggcagaaa caatccaagt cgatccaatc agtattcttg cctttggaga ttgtttgctg
    20161 attacagatg ggcatcacag ggcttatcag gctttattgg caggtcggga tacgatttct
    20221 gctgagtggg atagagatgg tggtgatgaa ctatatcatc tctatgcgca agcttgcgag
    20281 gaaagaaaga tttactctgt ttttgattta gaagatcgta tcctagctca agatgggtat
    20341 gaagcaaaat ggtataactg gtgtgatggt tttaatcaag cagcaactct tttgttgaaa
    20401 aggtagtgca ttgtgttttg ttccttatta taaggtcaat cattgtgagg aagcttttgc
    20461 ttggtatcag gatgtgaact tggtttacct cgtagatggg gtgaagcttc cttacagtca
    20521 agcgaccttg gaagccatgt attcctattt ggatcggcat ggtgagcttt tttggattga
    20581 agtcaaggag aagggagaat ggtttccaat tggggatatt acactatctc aggataatct
    20641 ccccattgtg attgggaatt ccgcttacca acatcgagga cttggaaaaa agattctaag
    20701 tgctttgatt gaattggctc gagtaaaagg atggaaagaa ttgagagtca agaaaatcta
    20761 cacctacaat catgcttcta ggaggtgttt caagtcgctt ggatttgtgg aaaatggagc
    20821 aacagaaaaa ggaatgagtt ttatattgga attagtctaa tcctgccact ttctccccct
    20881 taacctttga ctattccgca aaattatcgt aaaataaaga gtaaatgata aaatgaggtc
    20941 agagtctgtt cgctctggcg atagtagtat aaatgaggag aaacgctttg gaattagaag
    21001 tatttgctgg gcaagaaaaa agtgaactat ctatgattga ggtagcgcgt gctatcttgg
    21061 aacttcgtgg tcgcgatcat gagatgcatt ttagcgatct tgtaaacgaa attcaaaact
    21121 accttggaac atcaaacagc gatatccgcg aagctttgcc tttgttctac acagagttga
    21181 actttgacgg tagcttcatc tcacttgggg acaacaaatg gggtcttcgt tcatggtatg
    21241 gtgtggacga aatcgacgaa gaaatcatcg ctcttgaaga aaatgacgac gatgaagtag
    21301 caccaaaagc taagaaaaaa cgtgtcaatg cctttatgga tggtgattca gatgccattg
    21361 actacaatgc agatgatcca gaagacgaag atgcatacga agcagatcca gctctttcat
    21421 acgatgatga aaatccagat gatgaaaaaa atgaagtgga agcttatgat gcagaaatca
    21481 acgaaatcgc tccagatgac ttgggagaag atgtggatct caacgaagac gacgacgagt
    21541 tttcagatga cgatgctgaa accagcgagg aataagagtt agctattgac aattatccta
    21601 tttttaggta tcatattgtt tgggcacctc ttttagaggt cggggctccc tagttcttag
    21661 ggagctattt ttgttttttc aagaagttat cttcttgtat tttatactca atgaaaatca
    21721 aagagcaagc taggaaacta gccgtaggct gctcaaaaca ctgttttgag gttgtagata
    21781 agactgacaa agtcaggaac acatatctac ggcaaggcga cgttgacgcg gtttgaagag
    21841 attttcgaag agtattagtt gtgaatctgg tgcagtcgtc ccagattatt cttattagta
    21901 gggtcttgtt ttctatatcc cctcgtagtt aacaagacct tgagcatttt agaaagagga
    21961 atctatgtct acgaaatata tttttgtaac tggtggtgtg gtatcgtcca ttgggaaagg
    22021 gattgtggca gcgagtctag gccgtctctt gaaaaatcgt ggtctcaaag taaccattca
    22081 aaagtttgac ccttatatca atattgatcc gggaaccatg agtccttacc agcacgggga
    22141 agtttttgtg acagatgacg gagctgagac agatttggac ttgggtcact atgaacgttt
    22201 catcgatatc aatctcaaca aatattccaa cgtgacaact gggaaaattt acagtgaagt
    22261 tcttcgtaaa gaacgccgtg gagaatacct tggggcaact gttcaagtca ttcctcatat
    22321 cacagatgct ttgaaagaaa aaatcaagcg tgccgctcta acgaccgact ctgatgtcat
    22381 tatcacagag gttggtggaa cagtaggaga tatcgagtcc ttgccattcc tagaggctct
    22441 tcgtcagatg aaggcagatg tgggtgcgga taatgtcatg tatatccata caaccttgct
    22501 tccttacctc aaggctgctg gtgaaatgaa aaccaaacca acccaacact ctgtcaaaga
    22561 attgcgtggc ttgggaatcc aaccaaatat gttggttatt cgtacagaag agccagctgg
    22621 tcaaggaatt aaaaataaac tggcccagtt ctgtgatgtg gcaccagaag ccgttatcga
    22681 atcgttggat gttgaacacc tttaccaaat tccactgaac ttgcaggcac aagggatgga
    22741 ccaaattgtt tgtgatcatt tgaaattaga cgcaccagca gcggatatga cagaatggtc
    22801 agccatggtg gacaaggtca tgaacctcaa gaaacaagtt aagatttccc ttgttggtaa
    22861 gtatgtggag ttgcaagatg cctatatctc agtggtcgaa gccttgaaac actctggcta
    22921 tgtcaatgat gcagaagtta aaatcaattg ggtcaatgcc aatgatgtga cagcagagaa
    22981 tgtagcagaa ctcttgtctg atgcggacgg gatcatcgta ccaggtggtt ttggtcaacg
    23041 tggtacagaa gggaaaatcc aagccatccg ctatgcgcgt gaaaatgatg ttccaatgtt
    23101 gggagtctgc ttgggaatgc agttgacatg tatcgagttt gctcgtcacg ttttaggtct
    23161 tgaaggtgcc aattctgcag agcttgcacc agaaacaaaa taccctatca ttgatatcat
    23221 gcgtgatcag attgatattg aggatatggg tggaaccctt cgtttgggac tttatccgtc
    23281 taagttgaaa cgtggctcta aggctgctgc tgcttatcac aatcaagaag tggtgcaacg
    23341 ccgtcaccgt caccgttatg agtttaataa tgccttccgt gagcagtttg aggcagcagg
    23401 ttttgtcttt tcaggagttt ctccagacaa tcgtttggta gaaatcgtgg aaattcctga
    23461 aaataaattc tttgtagctt gtcagtatca ccctgaactg tcaagccgtc caaaccgacc
    23521 agaagaactc tacactgcct ttgttactgc agcagttgag aacagcaatt agcaaaatca
    23581 gaacctttga gaaaaatctc agaggttttt tgcatacgat gatattgcag tatatctgag
    23641 gtaggggtcc tctgtatgta cctgctaccg ttgaaatcaa tagcgactcc ctcttgccct
    23701 gtgctagtga atggatttat cagtatattg aaatgaaata aaatttgaac aaattaattc
    23761 ggaaagccaa atcaatttct agcaaagttt taggaactgg attgtatagt gaattgaaat
    23821 aagatgtgaa catctctatc aggaaagtca aattaattta tagaaatatt ttagcagtca
    23881 agatgtactg ttatagattc aatacattat acttttttaa tttaatccac tatagtaaaa
    23941 tgaaataata acaggacaaa tcgatcagga cagtcaaatc gatttctaac aatgttttag
    24001 aaatagaggt gtactattct agtttcaata tac
//
LOCUS       DBDESG010000030        23310 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.030, whole genome shotgun sequence.
ACCESSION   DBDESG010000030 DBDESG010000000
VERSION     DBDESG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.030"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..217)
                     /locus_tag="ACKB38_001189"
     CDS             complement(<1..217)
                     /locus_tag="ACKB38_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HGL7979959.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAA"
     gene            complement(286..555)
                     /gene="blpJ"
                     /locus_tag="ACKB38_001190"
     CDS             complement(286..555)
                     /gene="blpJ"
                     /locus_tag="ACKB38_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGL7979960.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1022..1219)
                     /gene="blpI"
                     /locus_tag="ACKB38_001191"
     CDS             complement(1022..1219)
                     /gene="blpI"
                     /locus_tag="ACKB38_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGL7979961.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1501..2088
                     /locus_tag="ACKB38_001192"
     CDS             1501..2088
                     /locus_tag="ACKB38_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGL7979962.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2015..2995
                     /locus_tag="ACKB38_001193"
     CDS             2015..2995
                     /locus_tag="ACKB38_001193"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HGL7979963.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFG"
     gene            3023..3658
                     /locus_tag="ACKB38_001194"
     CDS             3023..3658
                     /locus_tag="ACKB38_001194"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7979964.1"
                     /translation="MKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKN
                     IDKKVLRRHINYLPQQAYIFNGSILENLTLGGNHMISQEDILKACEVAEIRQDIERMP
                     MGYQTQLSDGAGLSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSL
                     TDKTILFVAHRLSIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3669..5030
                     /locus_tag="ACKB38_001195"
     CDS             3669..5030
                     /locus_tag="ACKB38_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGL7979965.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5087..5215
                     /gene="blpC"
                     /locus_tag="ACKB38_001196"
     CDS             5087..5215
                     /gene="blpC"
                     /locus_tag="ACKB38_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGL7979966.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(5286..6626)
                     /locus_tag="ACKB38_001197"
     CDS             complement(5286..6626)
                     /locus_tag="ACKB38_001197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGL7979967.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(6640..7377)
                     /locus_tag="ACKB38_001198"
     CDS             complement(6640..7377)
                     /locus_tag="ACKB38_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7979968.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(7382..7720)
                     /locus_tag="ACKB38_001199"
     CDS             complement(7382..7720)
                     /locus_tag="ACKB38_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGL7979969.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7996..8325
                     /locus_tag="ACKB38_001200"
     CDS             7996..8325
                     /locus_tag="ACKB38_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGL7979970.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(8493..9542)
                     /locus_tag="ACKB38_001201"
     CDS             complement(8493..9542)
                     /locus_tag="ACKB38_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7979971.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGTATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(9539..10270)
                     /locus_tag="ACKB38_001202"
     CDS             complement(9539..10270)
                     /locus_tag="ACKB38_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7979972.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRSKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            10338..10748
                     /locus_tag="ACKB38_001203"
     CDS             10338..10748
                     /locus_tag="ACKB38_001203"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGL7979973.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10758..11045
                     /locus_tag="ACKB38_001204"
     CDS             10758..11045
                     /locus_tag="ACKB38_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979974.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(11735..12871)
                     /gene="dnaJ"
                     /locus_tag="ACKB38_001205"
     CDS             complement(11735..12871)
                     /gene="dnaJ"
                     /locus_tag="ACKB38_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGL7979975.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(13482..13628)
                     /locus_tag="ACKB38_001206"
     CDS             complement(13482..13628)
                     /locus_tag="ACKB38_001206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979976.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     RELY"
     gene            complement(13630..15453)
                     /gene="dnaK"
                     /locus_tag="ACKB38_001207"
     CDS             complement(13630..15453)
                     /gene="dnaK"
                     /locus_tag="ACKB38_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGL7979977.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKTKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(15942..16466)
                     /gene="grpE"
                     /locus_tag="ACKB38_001208"
     CDS             complement(15942..16466)
                     /gene="grpE"
                     /locus_tag="ACKB38_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGL7979978.1"
                     /translation="MAQDIKNEEVEEVQEEEVVKTAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(16493..17527)
                     /gene="hrcA"
                     /locus_tag="ACKB38_001209"
     CDS             complement(16493..17527)
                     /gene="hrcA"
                     /locus_tag="ACKB38_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGL7979979.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(17691..18201)
                     /locus_tag="ACKB38_001210"
                     /pseudo
     CDS             complement(17691..18201)
                     /locus_tag="ACKB38_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            18330..18458
                     /locus_tag="ACKB38_001211"
     CDS             18330..18458
                     /locus_tag="ACKB38_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7979980.1"
                     /translation="MKIKNTFQLKELPPVTNFEKNGSRAKKRNKIGSIRIVKIRLA"
     gene            18809..21142
                     /gene="hsdR"
                     /locus_tag="ACKB38_001212"
     CDS             18809..21142
                     /gene="hsdR"
                     /locus_tag="ACKB38_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGL7979981.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATLKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            21155..22618
                     /locus_tag="ACKB38_001213"
     CDS             21155..22618
                     /locus_tag="ACKB38_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGL7979982.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCDFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            22618..>23310
                     /locus_tag="ACKB38_001214"
     CDS             22618..>23310
                     /locus_tag="ACKB38_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7979983.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILI"
BASE COUNT         7428 a         4715 c         4230 g         6937 t
ORIGIN      
        1 ttgctgcata ggctacacct ccaagtatag ctccacccgc agcaccagtt gctgcacctt
       61 gccatgttcc tgttttaatg cctagttgaa gacctcttgc tgctcctcct ccaacacctg
      121 ctttggcaaa atctccccaa ttgcatccgc caccttcaac gcaagcaagc atttcagtat
      181 ccataacaga aaattgtgac atcatttttg tatccatgac aaatactcct tttttaaaaa
      241 actaaaataa atcagaatag aatcctcata attttactat aagtcttacc aacttagtcc
      301 caatttatca ccaaccatac ctcctaagca tgttaatcca cccccaattg caccaatgtg
      361 tgctccaaca aatgcaccag caagtccagc tactcctaaa gtggccaaac ctgctccagt
      421 tccaccagtt ataattcccg tagtgactcc tgtaatcagt gcattttgac aatcagtgga
      481 gctatacccc ccttcaactt tcgcaagcat ttcagtatcc ataacctcta actgtgacaa
      541 catttttgta ttcatgatga atacctcctt tttattttca atttgttacc aaagtcttaa
      601 attcaataaa caaatagatt ttttatagta tctttttgat tttcttaaaa aagtatatac
      661 gtctactatc ttcttaaagg tagcagtacc tattttttag tctaagattt caataatctt
      721 gagtatctaa aatatcttaa tttcgttatt ctccttgcaa taaaaagttt tactatacta
      781 tttattaact tgcagaaagc aaaaaatatt agtaaataat agtttatagt taagtttttt
      841 attcctacca atccatcaac taagtaaagc atcaacgatt acataaacga ttgataatat
      901 aattaaaatt ttgctaacta tcttattctc atcattctta gataactttg atattttgta
      961 agtaagtaaa taagacagta aattaatagc gataataata ctatatttaa gaatcataat
     1021 cttacaaaga ggacataatt cctgaaccta cacaaataag tgttgctgct cccccagtta
     1081 tcggaccagt cgcagcagct aatagtactg ctccaataca accaccgatt gcagatccta
     1141 aattgcctct tcctccacta actatttcga gttcttcatt atccataaca gaaaattgtt
     1201 ccatcatttt tgtattcatg acaaatactc cttttttctt tttttatttt tgtcttgttg
     1261 taactttgat aagtttagta tatcatcgtt ttttaaaatt tttcatccag atcttgaatt
     1321 gtcatcgaaa cgtcttgaat tagctttttt atttcaagcc acctctaaat gtttaaaaaa
     1381 aataatttct aatcactttt ttaccattca ggaagtttta atgactattc aagatttcat
     1441 aaaatatgaa cttagtttta tgacataata gacctatcca ctatatgaaa ggaattgcca
     1501 atgacttctt ataaacgtac atttgttcct caaatagatg cgagagactg tggtgtcgct
     1561 gccttagcct cgattgctaa attctatggt tcagattttt ctctagctca cttgagagaa
     1621 cttgcaaaga ccaataaaga agggacgact gctcttggca ttgtaaaagc cgctgatgaa
     1681 atgggctttg aaacaagacc tgttcaagca gataaaacgc tctttgacat gagtgatgtc
     1741 ccctatccat ttatcgttca cgttaacaaa gaaggaaaac tccaacatta ctatgttgtc
     1801 tatcaaacaa agaaagacta tctgattatt ggtgatcctg acccttctgt aaaaatcact
     1861 aaaatgtcaa aagaacgctt tttctatgaa tggactggag tagctatttt tctagctacc
     1921 aaacccagct atcaacccca taaagataaa aagaatggtc tactaagcaa gcttccttcc
     1981 tctgattttc aaacaaaaat ctctcattgc ttacattgtt ctctcaagct tattggtcac
     2041 tattatcaat ataggtggtt cttactatct ccaaggaatc ttggatgaat acattccaaa
     2101 tcagatgaaa tcaactttag gaatcatctc agttggtctg gttatcacct atatcctcca
     2161 acaagtcatg agcttctcca gagattatct cctaaccgtt ctgagtcaga gattaagtat
     2221 tgatgtgatt ttatcctata ttcgccatat ttttgaactt cccatgtctt tctttgcgac
     2281 acgtcgtaca ggagaaatca tttcacgatt cacagatgct aactctatta tagatgcctt
     2341 ggcttctacc attctttctc tttttctgga tgtttctatt ctgattcttg taggaggcgt
     2401 cttactggca caaaacccta atctcttcct tctttctctt atttccattc ctatatacat
     2461 gttcatcatc ttttctttta tgaaaccttt cgaaaaaatg aaccatgatg tcatgcaaag
     2521 taattctatg gttagctctg ccattatcga agatatcaac gggattgaaa ctataaagtc
     2581 gctcacgagt gaagaaaatc gctatcaaaa tatagacagc gaatttgtag attatttgga
     2641 aaaatccttt aagctcagta aatattctat tttacaaacg agtttaaagc agggaacaaa
     2701 attagttctg aatatcctta tcctatggtt tggcgctcaa ttagtcatgt caagtaaaat
     2761 ttctatcggt cagctgatta cctttaacac acttttttct tactttacaa ctcctatgga
     2821 aaatattatc aacctccaaa ccaaactcca atctgcgaag gtcgctaata accgtttgaa
     2881 cgaagtctat ctagtcgaat ctgaatttca agttcaagaa aaccctgttc attcacattt
     2941 tttgatgggc gatattgaat ttgatgacct ttcttataag tatggttttg gatgagatac
     3001 cttaacagat attaatctca cgattaaaca aggagataag gttagcctag ttggagttag
     3061 tggttctggt aaaacaactt tagccaaaat gattgtcaat ttctttgaac cctacaaagg
     3121 gcatatttcc atcaatcatc aggatattaa aaacattgat aaaaaagtct tgcgccgtca
     3181 tattaattac ctaccccaac aagcctatat ctttaatggc tctattttgg aaaacttaac
     3241 cttgggcggt aatcatatga ttagtcaaga agatattcta aaagcttgtg aagtagctga
     3301 aatccgtcaa gacattgaaa gaatgcctat gggctatcaa actcagctct ctgatggagc
     3361 tggtctatca ggaggacaga agcaacgaat cgctctcgct cgtgctcttt taactaaatc
     3421 tcctgtttta atactagatg aagctactag cggtcttgat gtcttgactg agaaaaaggt
     3481 tatagataat cttatgtctc taactgataa aaccattctc tttgtagccc atcgtctcag
     3541 tatagccgaa cgaaccaacc gtgtcattgt tcttgaccag gggaaaatca ttgaagttgg
     3601 tagtcaccaa gagttaatgc aggcgcaagg tttctaccat catctattca ataaataagg
     3661 agaatgtcat gaatcctaat ctttttagaa gcgtcgagtt ttatcagaga cgttaccata
     3721 actatgcgac agtgttaatt atacctcttt cattactatt tactttcatc ttgattttct
     3781 cccttgttgc cacaaaagaa attactgtta cttcccaagg agaaatcgcc cctacaagtg
     3841 tcattgcctc cattcagtca accagtgata atcctatcct agctaatcat ttagtggcaa
     3901 atcaagtagt tgaaaaaggg gacttactca tcaaatactc tgaaacaatg gaagaaagtc
     3961 agaaaactgc cttagcaact caattacaaa gacttgagaa gcaaaaagaa ggacttggaa
     4021 ttttgaaaca aagcttagaa aaagcgactg atcttttttc tggcgaggat gaatttggct
     4081 accataatac ctttatgaat tttactaaac aatcccatga tattgaactg ggtatcacaa
     4141 agactaacac cgaagtttca aatcaagcta atctttccaa tagcagttca tcagctattg
     4201 aacaagaaat tacaaaagtt caacaacaaa ttggagaata tcaagagttg agagatgcta
     4261 tcataaataa cagagcacgc ttaccaactg gcaatccgca ccagtcaatt ttgaatcgtt
     4321 atcttgtagc ctcacaagga caaacacaag gaactgcaga ggagccattt ttatctcaaa
     4381 ttaatcaaag tattgcaggt cttgaatcat ctatcgcaag cctcaaaatt cagcaagctg
     4441 gtatcggaag tgtagcaact tatgataaca gtttagcaac caaaattgaa gtactccgca
     4501 ctcagttttt acagacagcc tcacagcaac aactaactgt ggagaatcaa ttaacagaat
     4561 taaaagtaca actagatcaa gccacacagc gtttggaaaa caatacctta acctccccaa
     4621 gtaaaggtat cgttcatctg aacagcgaat ttgaaggtaa aaatagaatt ccaactggta
     4681 cagaaattgc tcaaatattc cctgtcatca cagatacaag agaagtacta atcacttact
     4741 acgtatcttc tgactatcta cctctactag ataaaggaca aactgtaaga ttaaaactgg
     4801 agaagattgg aaatcacggc accaccatca tcggccaact tcagacaatt gatcaaactc
     4861 ctaccagaac agagcaagga aatctcttta aattaaccgc tcttgcaaaa ctatctaacg
     4921 aggatagtaa actcatccaa tatggcttac aaggtcgcgt cactagtgta actacaaaga
     4981 aaacatattt tgattatttc aaagataaaa ttttaacaca ttctgattaa ttttcagata
     5041 acactctata actatttatt atcttatcaa aaaggagaat cataacatgg ataagaaaca
     5101 aaacctaact tcatttcaag aactaacaac taccgaactc aatcaaatta caggtggagg
     5161 attgtgggaa gatttattat ataacattaa tagatatgct cattacatca cataagaact
     5221 tcatcatcca atacaactat aaaaaaataa gaccgagaaa caagtactct cggtcttatt
     5281 tttcatcatt ctgtatgtat cacagtaagt acctgacgaa agacttgatt ttgacaggtg
     5341 gtatttagac tggtattagg atggctttcc acaatcttca tgacggtata gagaccaact
     5401 cctctctcct cccctttaga actggctcca aaggagaaga tttcagaaat atcgatgccc
     5461 tcttctttga tggagttttc gatgataaag gtctcctgtg ctccattttt taaaaaggcg
     5521 attgaaacat gaggttgact agcttccaca ctggcttcaa tagcattgtc acaaaggata
     5581 gacacaatgg ttagaaaatc aagtagactc atcccctcga cctgaatctc ctcaggaact
     5641 tcgacattaa agacaatgtt cttatctctg gcttttaaaa atttccctgc tagaagactt
     5701 ttgagggctt tatcacgaat atttaccaat ctgcccaggt catatttatt gttctgcaat
     5761 ttctgactgg aatcctttaa gacggagcca tagacctctt ttatctgctc catatcctcc
     5821 tcttcaatgc ccagacgtaa gctagtcaag aggttggtat aatcatgacg aaagctccgt
     5881 acttccttgt aaagctcctc tatatgccga ctatagcgtt ccatatctct atagcgcagg
     5941 gcctgctctt gttccaatct ctcatagagt ttttccttca aataggtatc caatttcttg
     6001 ataaccccca taaaaaagag taggtaaaag actaggatga gatggcgaac agtctttgat
     6061 tgaatacttt gttcatattc aaaaaaagac agactttcca tgactagata gtagccaccc
     6121 attatccagt taatctgagt cagggacttt tgaaaggctt tatcgagaat ctcctttctc
     6181 aagctagtaa aatcgtagtc caaccatttc aaaaaagcta gagaaatgaa gaaattgaaa
     6241 attattatac ataacccagt aaatgagtag ccatcatata cttgcccttg tcccaaaaat
     6301 ggaagcacaa aataggagac tcctctataa aagagattca ccaatatcat tggaaagaga
     6361 ccataaaaga aaaggagttt tttaggaagc cctctcaata ataagaaaga taagcctatg
     6421 ccgtacaagg gttccataaa ataagatagg taaacatttc ctactatata gctaatcatc
     6481 acaaaaacaa aggccaacag tatcttcaaa agaaaggcct taaaaatcct ctcgaaagta
     6541 agatcaattc catccacctt aaagaagatg acaatttcta gtccattagt aacaagtgta
     6601 tacaacaata tccaagcaat gttcataaat tctcctagct cagtgtaatt tattgatggc
     6661 ctcagacact tccctgacct tataacgggc gattagacaa cttccaccat tgggaaagaa
     6721 aagcagtttt tctttcttat ccaaatgcac cacatttgca ggattgatga gaaaagagcg
     6781 gtggcactgc aagagacggg gctcctgctt gaaaacctcc tctaaactcg ctgtaaattc
     6841 cagcctgtct gtcttggtat agagaataac acgatgggct ctgggcgacg tttcgagata
     6901 gtaaacctct ttaaaaggat actgaaattg ggcaaatttt gatttaaagt aaaagcaatc
     6961 ttccgccaga cttttactat cttgactatt ggcatagagg agggctgtct cgatccgaga
     7021 ttcaaactcc tctgctgaca aggccttatc aatgtagtcc aaagcagaca cttggtagcg
     7081 aaaagacagg ggcataaact ccgagtgagt cgtcacaaag acaatcaggg cataaggatc
     7141 ccgatcccga atctttctag ccacttccag tcccttcatc tcttcatttc gaatctcaat
     7201 atccaaaaag aatagctgat gggccccctt ctcatgcact tcagccagca gttggtccgg
     7261 cttgccaaat acctcaaaag agctaggaat gatatgatgt gctttcaaaa gtttctcaat
     7321 cgtcgtttca attctagtct gttgggaaaa atcatcctct aaaacaaata ttctcatctt
     7381 cttactctcc ctctttcaac catttttgac gaatttctct atagcgacgt ctggcatact
     7441 tgacagcgta ttgaccttct tctccgagaa aaaggattct ttcttgaaaa tcaatcgatt
     7501 ttaatttatc aagattaacc aaacaatttc gatgacatct catcaaggtt tccccacatt
     7561 ggttctcaag attacttaaa ttttgaagca tattaaaact agcttcttct gtaacaatct
     7621 gtacggtatg ggctttagtt ggatgtgttt ggatatagta gatatcgtcg atgtttactt
     7681 tatagactgt tttctgcgtc tgaataatca tatatttcat tggattctcc tcctaagaaa
     7741 agaataacag aaaaggtcta gcccaagcac acccaatcct aaacagtctt tgaaaactcc
     7801 taaaaggatt attttctaca acatcatgat gagcttacaa ctattttatc ataaaattca
     7861 ctcagcgcaa ttcaagacat ttcaatgaca attaaagatt tgaataaaaa aaaagaatta
     7921 aaagtgataa actaacagta tcaaaacaca gttgccagaa gtaagactgg cacccagatt
     7981 aaaggaggca ttcccatgac agatacagac cccatcaaaa gagctcatac tttgattact
     8041 gacttaaaca aagcctacca agcatgcaaa caggcaagcg ctgacgacgt ccgctttcag
     8101 gagcaattaa actctattct tggttttcta gccaaggctg aaacagtgga taatcgattc
     8161 ttgattgaac tagaaaaatt ttaccagact tccagtcttc tcatgggact cagcgctctt
     8221 gatccagatg ctccaactcg cgccgcttgg cgggcctatg accgcttcca ctttgaccaa
     8281 gtcaagacca agttaatact caatgaaaat caaagagcaa actaggaagc tagccgcaag
     8341 ctgtacttga gtacggtaag acgacgctga cgtggtttga agagattttc gaagagtata
     8401 agtctctacg gaccaaccat tatcctgtag taacaaaaaa gaagttgaga cagactgaac
     8461 tcaacttctt ttttagtgtc gtatcagcaa tgttagtcct gcatctgacg ttttacctga
     8521 taaggcaaat acaagactag taaaaccaaa ccagctccta gtaaggctag aagggctagt
     8581 ttttcttgga aggtaatcaa cccaacaact aattccacaa gtaaaacaaa actggtcaat
     8641 cctcgaacta cctcctgtaa gcctttttcc ttttgcccct tgtccagcgg aaagagttgg
     8701 gtcaaatact ggtagtcaaa ggcatgatag agggccagca actggaagag caagaggtag
     8761 ttaaagagaa ctaccactgc tgtcgcaatc caagcttgct cgataaaaac ctgcgccagc
     8821 aaggaaagca agagaagacg aagactgaga gcaaagaggt cgccatttcg cagataagaa
     8881 cgcagataga gattttgcca aatcttccca ggcaccttct gaacagcctt taaaataaag
     8941 tccagatagg cacgacgctt aacgctgttt gaaattccct tgacctgcgt aaagagggca
     9001 aagaaacgaa gcaagacttg cttacgcttg ctttcttgag aaataacata gtcccagtcc
     9061 agtccagttt cagtgaaaaa tttgctggcc ttttgacaaa agtggaaata ttttcctacc
     9121 cccaataaaa gcacatagag cagaaaaact ggcaagccat aacccattgc taaaaataaa
     9181 ggcgcaaata acagcaagaa aagggtctgt acaaagagcc aaaagactag ggaaatgcca
     9241 gtttgacgct tgagatggag cttaatttcc tcttctccaa ctaagagaaa gagcttgtct
     9301 ggagcctcca tataggtggc agttcctccc caaagtaaaa gtaaaacaga cgtaattcct
     9361 acaaacaaaa ggataggcca atgattttca ggaaaatgtt gtaagagttg actgtactgg
     9421 taggctagaa agcccaacag gacaagcagg aacaagacaa agtggtcatt gagcacatag
     9481 cgcagataac caagacactc cttacgaaag gcctgctttc tctttaaaaa caagtctttc
     9541 atagatcctc ctctttggtc agagccaagt aaatatcatt caaactagcc tcaggcatat
     9601 caaaggcttc acgtagttgc aggagattgc ctttggaacg cacctctccc ttgtgaagaa
     9661 tgacaaaggc atcacacatc ttctccgccg aatccagcac gtgggtactc atgagaatag
     9721 acttgccctt ttgcttctcc acttccaaaa gctgaatcaa atcagaaata gccagcggat
     9781 caagaccaag gaaaggctca tccacgatga aaagacttgg atccaccaca aaagcacaga
     9841 taatcatgac cttctgcttc atcccttttg aaaaatgaac agggaaccag tctaatttct
     9901 gttccaaacg gaacattttt aacaagggct ctactcgttc gaaagccact ttttgctcaa
     9961 taccgtaagc catagcaacc gtttcgatat gctctctgag ggtcaattcc tcatacagac
    10021 taggcgtctc aggaatgtag ccaatctgct tgcggtagct agtcgcatct ccttgcagag
    10081 tcaggccatt gatattgatg gagccactat aaggtgccaa cagaccgata atctcattga
    10141 tcgtcgttga tttcccagca ccattgagac caatcaaacc gaccaactgc ccactttcaa
    10201 cagtaaagga cacatctttc aaaacaggaa catgaacata gccacctgtc aggtttttaa
    10261 tttctaacat attttctcca aatctggtat aatgtagcta tattatatca aaattcagta
    10321 cagtagaggt agattttatg tcagattgca ttttttgtaa aatcatcgca ggggaaattc
    10381 ctgcttcaaa agtatatgaa gatgagcagg tccttgcctt tcttgatatc tctcaagtaa
    10441 cactaggaca caccttggtc gtgccaaaag aacactatcg caatcttttg gagatggatg
    10501 ctacgagcgc cagccaactc tttgcccaag taccaaaagt agctcaaaaa gtcatgaaag
    10561 tcactaaggc tgctggtatg aatatcattt ccaactgtga agaagtcgct ggtcaaacag
    10621 tttttcatac tcacgttcac cttgtgcctc gctacagtgc tgacgatgac ctcaagattg
    10681 attttatcgc ccacgaacca gactttgaca aacttgctca agtcgctgaa accatcaaaa
    10741 acgcttaagg agttgctatg aaattatcca acctactgct atttgcagga gctgcagccg
    10801 gaagttatct ggttacaaaa aatcgccaaa ccatcacaga tgaagtcttg aataccactg
    10861 accgcgttca agctatcaag gacgatgtgg atattatcca aaacagcctg caaatcatta
    10921 accagcaaaa agaacttatc aaggaatacc aagaagactt gacttacaag tttaaggtct
    10981 tggaaaagga tatccagact agactagctg tgataaaaga aatgcaggga actgaagata
    11041 agtaaaaaga gcccgacggc tcttttttaa aacgtaaaca agttatagtg aattgaattt
    11101 agaatagcac accatagttt ctaaagcatt gttagagatt actttaaaat cctcttatca
    11161 atttgttcat attctatttc aatctattat atatatccca tcaactatgc atcataattt
    11221 aggtaattca tactcaataa aaatcaaaaa gcaaactaga aagctaggca cagactgctc
    11281 aaaacaccgt tttaaggttg tggatagaac tgacgaagtc agctcaaaca ctgttttgag
    11341 gttgtggata gaactgacga agtcagtaac catacccatg acaagccgac gctgacgtgg
    11401 tttgaagaaa tttttgaaga gtatcattca ccatttcaca actaaatcga agactttctg
    11461 ccgtaatgaa aacacctgaa acagcttggt ttcagctgtc cggaaacttt aaaacttagg
    11521 ttcaaagttt aggtatggaa cttcgaagga ggtcactacc gtccgtcatt acttaggaaa
    11581 gtcttaaaaa atctataaag taaaaagcaa actaggaaac tagccacggg ctgctcaaaa
    11641 cactgttttg aggttgtaga tacgactgac gaagtcagtc acatatatac ggcaaggcaa
    11701 cgctgacgtg gtttgaagag attttcgaag agtattattc tccatcaaag gcatctttaa
    11761 tatggtcaaa gaagcctttt ttctttggat ttactttcaa gtcaccagca gccgcgaatt
    11821 ctttcaaggc tactttttgg cggtcgttca agcctgtcgg tgttacgaca ttaacagtaa
    11881 cgtattggtc accaactgca ccgccacgaa ggctcggtgc ccccttacta cgtaggcgga
    11941 acttcttacc agtctgagtt ccctctggaa taaccaattc aacatcaccg tgaacagttg
    12001 gaatatctac tgtatcacca agagccgctt ggacaaagtt gaggttgaga ttgtagaaga
    12061 tagtcgttcc ttcacgttca aacttgtcgc tagcttccac agaaactact acatacaagt
    12121 caccataagg tccaccgtta aagcctgctt caccttgacc agcgaggcga atttgttgac
    12181 ctgtttccac accagcaggg attttcacat gtacgctatg agcttgtttc tcatgacctg
    12241 ttccatgaca ggttgtacat ggatatttga tttcttttcc tcgaccgtga cagacatcac
    12301 aggttacttg gcgacgcatc ataccaagag gagtctgcgt atcgacgtta atgacaccag
    12361 cgccatgaca gcgtccacaa gtgactggac ttgtccctgg cttagcacca gatccattac
    12421 atgtacgaca gccagcttca cgatgatact taacttcctt ctcagttccg aagatagctt
    12481 cttcaaaggt caaattgaca cgatactgga gatcatctcc ttggcgagga gcgtttggat
    12541 tgcgcgaaga accgcctccg ccgaagaaac ttgagaaaat atcctcaaaa ccaccgaagc
    12601 cacctgcccc attgaaaccg ccgaaaccac cagctccacc aaaaccacca ttggcgcctg
    12661 cagcaccata ctggtcatag gcagcacgtt tttggtcgtc actcaaagtc tcataggctt
    12721 cttgaacttc cttgtacttg tcctcagcac caggctcctt gttgatatct gggtgatatt
    12781 ttttggaaag cttacgataa gcctttttga tttcgtctgc cgaagcgttt ttggataccc
    12841 ccagacgatc ataaaattca gtattgttca taattcaaga tacaaagggc gtcaaacgga
    12901 cacagccatc taggagtttt gacgacggac gcttacgtcc tagaaaaaac tatctttttg
    12961 gcacagtcca tagcccgtgt tcagttacga acacccttat tcctttctat tgatgttctg
    13021 aatcgaacca ccatttaggt cgggttcaat tcctttcttt tatatttagt aagaaattct
    13081 gaaacccgtg ttcagttacg aacactcttt gttcctttct attgatgaga caaaacccaa
    13141 acaaatgaga ttccgtccct ttttatcgaa aaacagaggc tgggttgcaa cctctgcaaa
    13201 taagtaaatt aactttattt tgattttaaa tatcctataa gtacagaaat atttatatat
    13261 ttttgtctct tcttagtatt tgcctatata attccaattg atcataagaa atacaactac
    13321 tgtccaaatc ataccaatta tgaacggtat tacaaattta tatgtttgta tttctaatga
    13381 tatcatagct ggtaccatcg ccgatacaaa tgcagacaac aatactgagg taacaaaatt
    13441 ccttaccgct tcatcatttt tgatacaact atcatgttca gctaatataa ttctctatat
    13501 ctagctaaat attctattgg aatattatca ataaaatctt ttgatgttct atagttatca
    13561 gcttgattac aaaaatcatt ataaactttt cgtgtatttt ttagatactc ttcatccaat
    13621 acactcatct tacttttccg taaactctcc gtctacgacg tcatcgcctg cgttccctgt
    13681 tgcttgtgcg ccttctgctc cttcttgagc ttgttgcgct gctgcggctt gttcgtagag
    13741 tttaacagca agtccttgag ctttttcgtt caatgcttca agttttgttt tcatgtcgtc
    13801 caagttgttg tcttcttgag ctttcttaag gtcatcaagg gcagcttggg cagcgtcacg
    13861 ttctgcgtcg aagcctttac cttcagtttc cttgattgtc ttttcagtcg caaagattgc
    13921 ttggtctact tcattacgaa ggtctacttc ttctttacgt ttcttatcgg cttcagcgtt
    13981 tgcttctgca tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg agttcgattg
    14041 gatgacaata gtttgttctt tttgagttcc aaggtctttg gccttaacag acacgatacc
    14101 gttcttgtcg atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg cagctgggat
    14161 atcagtcaat tggaagcgtc caagagtctt gttatctgct gccattgggc gttcaccttg
    14221 aagaacgtgg atatcaacgg ctggttggtt gtctgctgct gttgagaaga cttgtgattt
    14281 agatgttggg attgtagtgt tgcgatcgat aagttttgta aatactccac ccattgtttc
    14341 gataccaagt gacaatggcg ttacatcaag aaggacaacg tccttgacat caccagtaat
    14401 cacaccacct tggatagccg cacccatagc aactacttca tcagggttta ctgatttgtt
    14461 tggttcttta ccagtttcag ctttaacagc ttcaacaacg gcagggatac gagttgaacc
    14521 accaacaagg ataacttcgt cgatttctga caagctcaaa cctgcatctg aaagggcttg
    14581 acgaactgga acttttgtac gttcaacaag gtcacgagtc aaatcgtcaa atttcgcacg
    14641 agtcaaagtc atttccaagt gaagaggtcc agcctcacct gcagtgataa atggcaagct
    14701 gatttgtgtt gaagttacac cagaaaggtc tttcttcgct ttttcagccg catctttcaa
    14761 acgttgcatt gccatcttgt cagtagacaa gtcgataccg ttttctttct tgaattctgc
    14821 taccaagtgg tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt tgtcccctgc
    14881 agttgacaat acgtcgaaga caccgtcacc caattcaagg atagagacgt cgaatgtacc
    14941 accaccaagg tcaaatacca agattttttc ttctttgtca gtcttgtcca aaccataagc
    15001 aagagctgct gcagttggtt cgttaacaat acgttctact tcaagaccag caattttacc
    15061 agcgtctttt gttgcttgac gttgagcgtc gttgaagtaa gccggaactg tgataacagc
    15121 tttggttact ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt gaaggatcat
    15181 agctgagatt tcttgtggag tgtattcttt tccatttgca gaaacttttt cagaagttcc
    15241 catcttagat ttgatagaga taactgtatc tgggtttgta actgcttgac gttttgcagc
    15301 atcaccaacg atgatttctc cgtttttgaa tgagactaca gatggagttg tgcggtttcc
    15361 ttctgggttt gcgatgattt tgctttcagt tccttcaaga actgcaactg ctgagtttgt
    15421 tgtacctaag tcaataccga taattttaga catgtgtttt tctccttgtt agttaatttt
    15481 ctatttttct ttttgatact cttcttaagt ggcgacagtg gcgacgccgt cagagcttga
    15541 cttcgtcaat ctctttgact aaactttgag cctaaggtct caaaatttgc gcaaatagcg
    15601 ccacggcgaa gagtatcgtg tttggttcgc ttcgctcact attacaaagc caaacatatt
    15661 ttatctttct tcatagttta ttgtcgtttc ggacaagttt tctattatgt aaattgcgac
    15721 acgaggagtc gaaatcgatt ttatttcgac gacgagttag taaggaagct aggcaaacgc
    15781 catagcgatt gccgttttct gacgagttgc tttagctacc gtcagaattg ccgaatcgaa
    15841 cacgccctaa gctctgagta aaaaagatag attttcttgt gttcatcgaa cacttcgtca
    15901 atctcctatt tttactgcga gctttttacg ggctttatat cttagttata caccactacc
    15961 attgctgggc gtaggatgcg gtcatggagt ttgtagcctt tttgaaagac ttgagcgatg
    16021 gtatctactg ggtgttcatc gtctgctggg agagtttgga tggccatatg gtagttatgg
    16081 tcaaattcgc catctgctgc gatttcttca attccttctt ctttcaaagc gtgaatcaag
    16141 ctttcttgca ccatccccaa gcccttcttc acatcatctg tcaaaccttc aactgcaagt
    16201 gcacgctcaa ggttgtcaag agatggtaag attgcttttg ccaagtcctg gctacgataa
    16261 cgttgcaagt tttgacgttc ttcattggca cggcgttgga tattttgcat ttctgcatga
    16321 gcgcgaagat atttgttttc gaactcatct gcacgttcat ttgccaagtc caactcagac
    16381 ttttcaggag ttgtttcttc agctgttttc acaacttcct cttcttgaac ttcttctact
    16441 tcttcatttt ttatatcttg ggccatgttc gcctccttta atgatttcaa tcttaatgta
    16501 cttcgtaatg attactgctg aggtagcggt aaaaatctgt caacttcatg gtcaaaacac
    16561 ggttgaccac attgacttga ttgattagct gttggtaatc cagattaact ggaccgataa
    16621 tggctagaat tccaactccc cgataaggaa tgaggaactt actactgatt accgctaggt
    16681 cagctaaaca ggactcttga ccgtctgcaa cacgaacatt ttgcatctga tcctcacgca
    16741 acccctcacg aatctccaag gccacctttt gcggttggtc aaagaactga taggctgcta
    16801 gattggcaaa attcaagaga tggaccttgc ccgccatcac aatgttttcg ttgaacattt
    16861 ccttaaagat gtgttcaaag agatcgatga cattatccgt tgttgtaaag taacgctgga
    16921 taatctgcgg aatctccgtc cgaatcttgt agtgaatatc taaaacggtg tgaccgagga
    16981 aacgttcctg aatgatgctc ttcagtttca gcaaatcctc ctgcaagaag ttccttggaa
    17041 tcagaaactg actagtaacc gttcgcgact cgtctagggt aaataccgcc aaggctgtat
    17101 gttgccccaa aacaacgata tcaaaggctg tcaaacgttg cctgctcggc tcaacatcca
    17161 gtgccactac cgtacagcca ctcaggtctg ttagtaagtt agcagcctct tgcagaatat
    17221 cctccaattt gaagaattcc tgatcaaagg ctttgacaat ctcatatacc tcattttcag
    17281 ccagccggtc aaaatccagt gagtgtttca catagtactg aaaaccagca acacttggca
    17341 tccgaccact tgaagtatga gccttctcaa gcaacccttg tttttctagt tccgccatgt
    17401 cattacgaat ggttgcactg ctagagttaa tagactcttg caaggctttt gatccgacag
    17461 gttcgtgcgt tttggtaaag atgtcaataa tcagatttaa aatatcctgc tgacgctctg
    17521 taaccatctc atcactcctc tctgtctgat caattagcac tcaatgcatc caagtgctaa
    17581 ctcatgattc tattatacac ccttagaaga gaatgtcaag acaaaaactc aaaaaattag
    17641 cactctttta tcaagagtgc taaaaatcaa tctgaagacc tagaaccttg ctattcatct
    17701 actcggtatt tcctttttag tctgaaataa ccataaatta gataagaaat cagaaaagca
    17761 tataaaacaa aaataagaaa gaaagacata gaaagatcct ctctaaacaa actcatacaa
    17821 acaacaagac cacctgagaa agtagctgta caggaacgaa gtctagatcg cataacttcc
    17881 catttgagcc ctttactcat atagacttga tcctctggaa gtaaatcaga agacgattta
    17941 cgaagaatcg aacaagaacc tgctcctatc aattcccaac ctctatctct aaaatcttgc
    18001 agttcatgct tatacttttt taagaaatct agaatcatag atacggtaga tgacatcgtc
    18061 tggttgacat tggtcaaaat agaacaaacc aaaacgactc gttctatacc tccaaccttt
    18121 caaatgcatc tcatgtaaat gttcttcttc cttgtccaaa tcaacaatgg tgaaaatccg
    18181 aaattctact ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt
    18241 tattagataa ttctttccac ttttgactca atctccaaaa aatataagaa atctgaatcg
    18301 caaaaactat caataaaacc caatctatta tgaaaatcaa aaacactttc caactgaaag
    18361 aactacctcc agtgacaaac tttgagaaaa acggtagtag agctaaaaag agaaataaaa
    18421 taggaagcat ccgcattgtt aaaatccgtt tggcataaaa aaatctttat ttaaacgaaa
    18481 atattatggc aaaatttacg ccagtttttg aacggctgat gtagatattt tatactttca
    18541 aaatgtttaa atgtgattat ttatttttga aaaatagatc accagcccga ctgaaagtgc
    18601 ttatagaatg ataataagtc gcctgccgaa aacagcgaaa aatagcggtg ttatgcggag
    18661 ataatctgac gcgatgcgaa agtatattgc atacttattt tcaacaattt agcagagtat
    18721 ttttataagt gtgatataat agaagtataa tttgttctga tagtttattt tatggagaag
    18781 tagattttta gaatgcggag ggttcaatat ggttgagttt ataaagtcta agaaagaaat
    18841 gagtgaggag gatattaaag caaatttcat cactcctgct attgtatcca aaggatggaa
    18901 aaatggtgag catatcgctt acgaagaata cttcactgat ggtcgaattg aagttagagg
    18961 agataaggct cgtcgtaaag aaggaaaaaa atcagactat tcactgtatt accaatttgg
    19021 aactcgaatt gcaattgttg aggcaaagga taataaacac agcgttcgag caggattaca
    19081 acaagctatt gaatatggag agattttaga tgttccattt gtttattctt cgaatggtga
    19141 tggctttatt gaacacgacc gtatcacgag agaagaacgt gagctggagt tagacgaatt
    19201 ccctactcgt gaagaattat tttctcgtat gacgaaggaa aaaggattga cgtacgaaat
    19261 tacagaagct atctcaactc catactatac agacgccttc tcaatgaaaa cgccacgcta
    19321 ttatcagcaa atagctatca accgtactat tgaaacagtt gccagaggac aaaaacgagt
    19381 aatgtttgtg atggcaacag gaacggggaa aacgttcatg gcttttcaaa ttattcatcg
    19441 ccttcgaaaa gctggtttgg ctaaacgagt tttattctta gcagatagaa acatcttagt
    19501 agaccaaacg atggctgaag actttaggcc attcgaaaag gtaatgacga aaattacacc
    19561 aaaacttttg actgctcctg aaaaattaaa ttcttttgaa atttatctag ggctttatca
    19621 gcaactaact ggtgaagatg gaactgaaac acattatcaa aaatttgaca aagacttctt
    19681 tgatttaatc gtaattgatg aagcgcaccg tggttcagct aaggaaaaca gtaactggcg
    19741 taaggtaatt gattatttca gttctgcgac acagattggg atgaccgcta ctcttaaaga
    19801 aaccaagaat gcttccaata cggaatactt tggtgagcca atctatactt atagtttaaa
    19861 acagggaatc gaggatggtt ttttggctcc atatcgtgtt atgagggtta atttagatgt
    19921 ggatgtggat ggttatcgtc cagaaactgg aaaagttgat gctaacggac aattaataga
    19981 agataggtac tacggcagga aagattttga taaaaccatt gtcattgatg atagaacgca
    20041 aagagttgcc aagtttgttt ctgattatat gaagcaaaac aatgcacgat ttgataaaac
    20101 aattgttttt tgtgttgata ttgaccatgc cgagcgaatg cgtgctgcac ttgtaaaaga
    20161 gaatctagac ttagtccaag aagactatcg ttatgtcatg caagtaactg gtgacaacgc
    20221 tgaaggaaaa gctcaactgg ataactttat ggatgtcaat tctaattttc ccgctattgt
    20281 aacaacgtct aaattattaa cgacaggagt taatgctaaa acatgtcgtt tgattgtttt
    20341 agactctaat atccaatcca tgactgaatt taaacaaatt attggtcgtg gcacacgtct
    20401 ttatcctcaa aaggggaaag aattttttac gattattgat tttcgaaatg ttaccaattt
    20461 gtttgctgac cctgattttg atggtgatcc agtgaaggtg ctagaaacag gtgcgaaaac
    20521 agtcagtggt tctacgcccg gtttcgtaga tgaggaaggt gacccagtag aaaaatatat
    20581 cgttacagac aagcaggtta ccattcttaa ttctactgtt caagtattgg atgaaaacgg
    20641 gaaactgatt accgaaagcc tgaccgacta cactcgaaag aatatcttag gtagctacgc
    20701 cactttgaac gattttatca cagtttggca tacggcagat aagaagaagc ttatcttaga
    20761 cgaactttat aaaaaaggag tttatctaga tgctattcga gagtcggagg gaatatcaga
    20821 acaagaaatc gatgattttg atttactcct aaaacttgcc tatggtcaaa aagaattaac
    20881 caaaacggaa cgtatcaata aactcaaaca aagcggatat ttatataaat atagtgagga
    20941 agcgcgtgct gttttggaaa ttttactgaa caaatacatg gataaaggta ttggagaact
    21001 cgaaagcatt gaaacattaa aacttccaga atttcagata tatggtggaa ccttcaaaat
    21061 catcaatact tattttggag ataaaaaacg atatttacaa gcaattaaag aattggagca
    21121 agagctattt acagtagctt aatgaaagga aagtatgtca attacatcat ttgtaaaaag
    21181 aattcaagat atcactcgaa acgatgctgg tgttaatggt gatgctcaac gtattgagca
    21241 aatgtcttgg ttattattct taaaaattta tgatagccgt gaaatggttt gggaattaga
    21301 agaagacgag tatgagtcaa ttatcccaga ggaattaaaa tggcgaaatt gggctcatgc
    21361 tcaaaatggg gaacgggtat tgacaggcga tgaattactt gattttgtca ataacaagtt
    21421 attcaaagag ttgaaagagc ttgaaataac ttcaaatatg cctattcgaa aaacgattgt
    21481 taaatcagct tttgaagatg cgaacaacta tatgaaaaat ggcgtcttgt tacgccaagt
    21541 catcaatgtt attgatgaag ttgatttcaa tagccctgaa gatcgtcatt cgtttaatga
    21601 tatttacgaa aaaattctta aagatattca aaatgctggg aactcaggag aattttatac
    21661 gccacgtgca gcgactgatt ttattgccga agttcttgac ccaaaacttg gagaatcaat
    21721 ggcagacctt gcttgcggaa caggaggctt cttgacttcg actctgaacc gtttaagtag
    21781 tcaacgtaaa actagtgaag ataccaaaaa atataataca gctgtttttg gtattgaaaa
    21841 gaaagcattt cctcatcttt tagcagttac aaatctgttt cttcacgaaa ttgatgaccc
    21901 taaaattgtt catggaaata ctttggagaa aaatgttcgt gaatatacgg atgatgaaaa
    21961 atttgacatt attatgatga atccaccttt tggagggtca gaattagaaa caataaaaaa
    22021 taactttcca gcagaattac ggagttctga aacagctgat ttatttatgg ctgtcattat
    22081 gtatcgtttg aaagaaaatg gtcgtgttgg agttatttta cctgatggtt ttctatttgg
    22141 tgaaggtgta aaaactcgct tgaaacaaaa actggtagat gagttcaact tgcatacgat
    22201 tattaggttg cctcatagtg tctttgcacc gtatacagga atccatacga acattctttt
    22261 ctttgataaa acaaagaaaa cagaagaaac ttggttttat cgtttagata tgccagatgg
    22321 ttataaaaat ttctcgaaaa ctaagccgat gaagtcagaa cacttcaatc ctgttcgtga
    22381 ctggtgggaa aatcgtgaag agattctgga aggtaagttc tacaaatcta aatcatttac
    22441 acctagtgaa ttggctgagt tgaattataa tttagaccag tgtgactttc caaaagagga
    22501 agaggaaatc ttaaatccct ttgagttgat tcagaattat caagcggaaa gagcaacttt
    22561 aaatcataag attgataatg tattagctga tattttgcag ttgttggagg acaaataatg
    22621 acaccagaac aacttaaagc aagtattctc caaagagcga tggaagggaa attagtgccg
    22681 caaaatccca atgacgaacc tgcaagtgaa ttattaaaga gaattaaagc tgaaaaagaa
    22741 aaacttatca gtgaaggaaa aatcaaacga gataaaaagg aaactgagat atttcgtggt
    22801 gatgatggga aacattatgg gaagtttgct gatggaagca ctcaagaaat tgatgttcct
    22861 tatgatattc ctgatacttg ggagtgggtg aggttttcta cattggttga aattgtcaga
    22921 ggtggctctc cacgaccaat caaagattat cttacttctg aagtagatgg aataaattgg
    22981 ataaaaatag gtgatactga aaagggtgaa aagtatataa ataatgttaa agaaaaaatc
    23041 aaaaaatcag ggcttaacaa aactagattt gtaaaaaaag gtacattttt gttaactaat
    23101 tctatgagtt ttggtagacc ttatattttg aatgttgatg gtgcaataca cgatggatgg
    23161 ttggctattt cgaactatga aaactcatta aataaagatt acctattcta tattctttca
    23221 tcaaatgtag tttattctca atttctatct ctaattagtg gagctgttgt gaaaaacttg
    23281 aatagtgata aagttgcttc tattcttatc
//
LOCUS       DBDESG010000031        22987 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.031, whole genome shotgun sequence.
ACCESSION   DBDESG010000031 DBDESG010000000
VERSION     DBDESG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.031"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(131..355)
                     /locus_tag="ACKB38_001215"
     CDS             complement(131..355)
                     /locus_tag="ACKB38_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HGL7979984.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFILLLTFMVITYK"
     gene            complement(360..539)
                     /gene="asp4"
                     /locus_tag="ACKB38_001216"
     CDS             complement(360..539)
                     /gene="asp4"
                     /locus_tag="ACKB38_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HGL7979985.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="ACKB38_001217"
     CDS             complement(541..1878)
                     /gene="gtfB"
                     /locus_tag="ACKB38_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HGL7979986.1"
                     /translation="MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="ACKB38_001218"
     CDS             complement(1871..3382)
                     /gene="gtfA"
                     /locus_tag="ACKB38_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HGL7979987.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="ACKB38_001219"
     CDS             complement(3394..5766)
                     /gene="secA2"
                     /locus_tag="ACKB38_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HGL7979988.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIHADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="ACKB38_001220"
     CDS             complement(5759..6199)
                     /gene="asp3"
                     /locus_tag="ACKB38_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HGL7979989.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKVKDFIFTYPEDSYTYRVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="ACKB38_001221"
     CDS             complement(6196..7731)
                     /gene="asp2"
                     /locus_tag="ACKB38_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HGL7979990.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="ACKB38_001222"
     CDS             complement(7715..9295)
                     /gene="asp1"
                     /locus_tag="ACKB38_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HGL7979991.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLEMDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIVDDRGFVSSIIF
                     FEDGQAAYQEYLNLKGEWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="ACKB38_001223"
     CDS             complement(9305..10522)
                     /gene="secY2"
                     /locus_tag="ACKB38_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HGL7979992.1"
                     /translation="MTKIYSSIAVKKGLFTSFLLFIYVLGSRIILPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLLIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(10579..11483)
                     /locus_tag="ACKB38_001224"
                     /pseudo
     CDS             complement(10579..11483)
                     /locus_tag="ACKB38_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(11483..12679)
                     /locus_tag="ACKB38_001225"
     CDS             complement(11483..12679)
                     /locus_tag="ACKB38_001225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGL7979993.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGIDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12688..13908)
                     /locus_tag="ACKB38_001226"
     CDS             complement(12688..13908)
                     /locus_tag="ACKB38_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGL7979994.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAATFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13939..16383)
                     /locus_tag="ACKB38_001227"
     CDS             complement(13939..16383)
                     /locus_tag="ACKB38_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HGL7979995.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQYIEQLDYLAGQLPNVVFQIAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHELEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16393..17403)
                     /locus_tag="ACKB38_001228"
     CDS             complement(16393..17403)
                     /locus_tag="ACKB38_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGL7979996.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARSLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(17432..18022)
                     /locus_tag="ACKB38_001229"
     CDS             complement(17432..18022)
                     /locus_tag="ACKB38_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGL7979997.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(18037..18309)
                     /locus_tag="ACKB38_001230"
     CDS             complement(18037..18309)
                     /locus_tag="ACKB38_001230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7979998.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGRTLLL"
     gene            complement(18314..19528)
                     /locus_tag="ACKB38_001231"
     CDS             complement(18314..19528)
                     /locus_tag="ACKB38_001231"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGL7979999.1"
                     /translation="MNTKSIVFNADNDYVDKLETAIKSICCYNNCLKFYVFNDDIASE
                     WFLMMNKRLKTIQSEIVNVKIVDHVLKKFHLPLKNLSYATFFRYFIPNFVKESRALYL
                     DSDIIVTGSLDYLFDIELDGYALAAVEDSFGDVPSTNFNSGMLLVNVDTWRDEDACSK
                     LLELTNQYHETAYGDQGILNMLFHDRWKRLDRNFNFMVGMDSVAHIEGNHKWYEISEL
                     KNGDLPSVIHYTGVKPWEIISNNRFREVWWFYNLLEWSDLLLRKDIISRSFEELVYSP
                     KAHTAIFTASCEMEHVEYLIENLPEVHFSILAHTYFASSVVALLRYSNVTIYPCFSPF
                     DYRKILDNLDFYLDINHYKEVDNIVSVVQQLSKPIFTFENTSHDIGNQTNIFSSTEPN
                     KMVEAIRQFIGE"
     gene            complement(19835..21919)
                     /locus_tag="ACKB38_001232"
     CDS             complement(19835..21919)
                     /locus_tag="ACKB38_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGL7980000.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIITQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHILGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISAWGKLYKARLFEQLRFDIGKLGEDGYLNQKVYLLSEKVIYLNKSLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     ATYKEFEMKQRLLNQLSRQEESEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFVQEDK
                     ALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDVTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYAVDQV
                     QAMIEKLREISK"
     gene            complement(21980..>22987)
                     /locus_tag="ACKB38_001233"
     CDS             complement(21980..>22987)
                     /locus_tag="ACKB38_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142291904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGL7980001.1"
                     /translation="SASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASISASESA
                     STSASASASTSASASASTSASVSASTSASASASTSASASASTSASASASISASESAST
                     SASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSVSN
                     SANHSNSQVGNTSGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEE
                     E"
BASE COUNT         7328 a         4870 c         3627 g         7162 t
ORIGIN      
        1 tatctacaac ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt
       61 tgattttcat tgagtataaa aaagaaaact tcagaccaat ccacaaaagg tgaatatctg
      121 aagttttctt ttatttataa gtaatcacca taaaggttaa tagtagaata aataaagcca
      181 aactcaccaa taaagtgagc accttgacca aggtgttgct ctgccagtag cttggtttac
      241 caatattact agtggcatcc atggaaaata gttgattttg acggggttgt atagttacca
      301 atacaatcaa agcaagagat aggataatag ctaaaacaat tagtatttca atcatagatc
      361 tacctcaaaa ttcccagcaa agtaaagagc aaaccaatca gaatcatcaa acccagtaaa
      421 agtgaaaaag tcttactaat cttttcccct cgggtttctt tttccttctt gatgggtttt
      481 tgtttcaact cttccatgcg gccttcaacg tctttgtaaa ataaatcttc ctctgacatg
      541 ttagcctcct aaaacagttt gcatggtttc ctgatatctc accaagtcaa cataatttgc
      601 atgttggcct tgtttgccaa gtgcctgacg catttgatca acatctgaaa gggccaattt
      661 aatggtctca accaaagcag caacttcact actttcaaat agatggtctg gagcgatata
      721 aagtctattg tgcaccgtct gattaaagcc aagaatcaag agattgtgct caaaggcctg
      781 acgcactgcc tgtagcaact cattactgtg gtttatatcc aagtaaatat ccgacagttg
      841 atacagctcc tgaatcttct gtggactagc gttctggtaa agggccacat taggatagca
      901 aagcatgtct aagagcttag aagacatctc tgtcaccgct gcaatacgga aagtgacatc
      961 aggcaaggct cctgcgattg cttctacttg ctcaatctga tctgaattgg tcaagattaa
     1021 ggcatctcgt cttaggaaat tatcacgttt gaactggtaa tgataaccca agtgcacaaa
     1081 ctgatcatgg tatttctcgt cagttaactc taaagcgcgc tcataagtcg ccttatttgg
     1141 aatgatgatc ttcttagtac gcacattatc actttccaaa atcaactgca tattacctgg
     1201 aatggcatca tagagaggtt cctgccatac caagacatcc gagccagatt tatctggatg
     1261 atggaaggaa accaagaaag gagtcgctag agtattaaag ataagctgac tggtatctat
     1321 ttccaaatct tgcaaaaaga aggtgataaa ttcaacttta tttgcaaagt aacgcatgga
     1381 ctgacctggc aaagtcaata agatatcacc cgtcacatgg ttttccagta aaacttgttg
     1441 accattgaca tcttggtaaa ctgtcataat cggctcgcta tctgcgctat aagtcgttgt
     1501 agcaaaacaa gctccgaagc gattgtagtg gtcaacctga cgtactcgac cttctaggtc
     1561 tttccagtct acctgtttaa ccaagcgagc ctgcattcca tcagcataat gaatgacagc
     1621 cctctcctgc gtcacatctt caatacaagc agactgatta tctcctaaaa tttcccaaaa
     1681 atctgaaacg ggaacttgat taaaatagag aggttttcca tcctcgtaac ctagataata
     1741 ggtaaaagga gaaagcagac catcaggcag aaaaccatct gcatcgatga ccactccaag
     1801 ttgagaaaga ccagtagcgc ctagactttc atgtaaatct cgactttctt gactgtaact
     1861 atcataaagt tcaatcatgg agcacctcct ctactgtttt cttccacttt tctaaaattt
     1921 ctttggtcaa gaagccttct gcaatttggt aagaataggc acgcatagct tccaaacgat
     1981 tttcttgata caattgacaa atcttagcgg cataagcttg cttgatttgg tcttctacat
     2041 ggtcagatga acttggaatc aaataaccat tttgcccatc ctctataaag gtctgattac
     2101 cataaggcac atcaaaacca attagaggta gacctgaacc aatagcttcc atcaaggtca
     2161 gaccaaatcc ttcgctggta gaagccgtta agtagacctc atactggcta taaatctgcg
     2221 aaagttccgc atgccccttg agttggatat agtcctctgc ctgatgattt gcaataattt
     2281 ctctaagcag agaatcttct ccaccactac catagatatc aaaggttagt tccggtaact
     2341 ccttatgagc ttcaatcaca gctttcacaa gccaatcaat gtgcttttct ttggcaagac
     2401 gtgaagccgt aatcaatgaa aatggtttgc gcccttgact tgaatctgtc aaggaatcaa
     2461 tactgcctac aggaatggta acaatctttg gctgatgctg agtatatttg gcaaattgct
     2521 cttgtagaac ttcattttgt ctatcagtag acacgataaa gaagtcaacc ttatctgcat
     2581 tggtaaactg atagtcataa tagttattcc aaaggatata gtcctcattt gtagcatttt
     2641 cactataatg ctccgcatga acaactaccg ctagatgtgc tgtctgtgct tcctcaaaca
     2701 caacctgtcc aatacctgtc tccctatcga gaatgaccaa atcagactta ttcaaattca
     2761 aagatttcat aaaggcacgc acaaaagctt gctttccata gaaaatctta tccttgaaat
     2821 gataaacttc ttccttccct tgattcatca agatatcata gactggagtc ccgtcttcat
     2881 tataaaaagt tcgttggtat aagactgcaa cattgtcctt gggagcaaaa tactcgctac
     2941 aataacgcgt ataagaaaag taatccttcc gaatcaggtt tcccttaaaa acatactcgg
     3001 catgttgaac caagtccttg ttctcatcaa ccaaataaca ggttacaaac ttatcttggt
     3061 caaaaaagaa tacacgtaaa accttgccat ttttttctct gtgactttct tcaccaccaa
     3121 agtaagccaa gacatcatcc actgtcacgc tagtaggtgc aattttgata tctgtgaaat
     3181 gattataaag ccagataacc tgattatcat caaaaccaat attggctgtt aagtgctgaa
     3241 tattatcggc taaaatcata tctgtaaaga taaacttaga ggacagattt aatttccgaa
     3301 aaacaccagc acgataggct tgagcgtatt caacaccgct actagcccaa ccaattccta
     3361 aatttatatt gtaaattgtc atattttctc cttttatgga aaatgagtca cgatttcacc
     3421 ttttggagtg acctcaacca gccccatcag gagattacgc accatatccg catgaatctg
     3481 ttctttcata gcttcaaagc ccgcgtaggc ttcttgatag tactctacga ttggattttt
     3541 ctgactagca gattgaccac cgatagccat ggatagctgt tgtagatagt ctacctgctc
     3601 tacccagttg tcatcaatgg ctttaagcag tgaaagtcgt aaaaactgtt catataagtc
     3661 atgttgatta agtaattctt tcttttcaga aagttcttta tcaatcacct gcttcataaa
     3721 gctacgaact gcagttttgt cagttacatc tatataatct ggaacctctt taacatgaaa
     3781 actaatattg gtcacaataa agtgaaacaa taattcacga ctagcatagt gatcagccgc
     3841 tacctcttct gtatatctct caatgatatc cacaacaaca tcctctaagt cacgagaacc
     3901 atctattaga cgatttctct ctttatagac tatatcccgt tgtatattca tactttcagc
     3961 atactccaga gtctgacgac gtgctgaacg tccagcacta tcactggcat gctgagcctt
     4021 ttcgactagt ttccggtatt tacgaccttt caatacttcc ggttgagtca tatcttgaac
     4081 ctgatagtct ttgtactttt tatgcaccca agatggacca aatttcttga taacatcatc
     4141 ctctaaggat acaaaaaatt tactcatacc aggatctccc tgacgaccag aacggccacg
     4201 aatttgtagg tcgatccgct gactttccat ccgctcagtc ccaataacaa tcaagccccc
     4261 aagctctgcg actcctttac caagcttgat atccgtacca cgtcctgcca tagaggtagc
     4321 cactgtcaca gcccccatct gacctgactc ggagataatc tgagcctcac gcgccgcatt
     4381 attagcattt aggacattat gggcaatccc ttcacgaaac aagagagacg aatagagttg
     4441 agacatttca actgagccta caaaaacgag taaaggattt cccttagcat ggtattgctt
     4501 gatgtactcc aaggatgcat acactttttc aggtaaagtg atatatagat tatctggata
     4561 gtcaatccgt tgtctcggac gattggttgg aatgcgtact acagacatat tgtaagtttc
     4621 aataaactct ttttccgcga ccttacctgt ccctgtcata ccagatatct tattaaacat
     4681 cttaaaaaga ctctgatagg tgatcgaggc catagcccgc gtctcaggag ataatttgac
     4741 atgttccttg gcttcaatag cctgatggag acctccttga agtttagtca tttccattag
     4801 acgccctgtt cccttatcaa ccagtaccat ctcatttcca cgaatgatat agtccttatc
     4861 tttagtaaag agcttatgag ctcgaatcgc ataaaccaaa tgacgagcaa aagacgcatg
     4921 ctcttccttg tataaattat caatccctag gaaattctca gcagacttgg cccccttagt
     4981 agtgagccaa acctcctctt tctcctcttt aaagatataa tcctctcctt cgaccaaggt
     5041 tgttacaagt gtatcaatga tcgcatagta attagactga acacgaggag aacccgcaat
     5101 aatcagagga gtttgtgcac tatcaagcaa gatatcatca atttcatcaa taatcacata
     5161 gttaaacggt cgtaaaaact taccttcttc attcgaggct aggttatcat ttagataatc
     5221 aaaacctaaa ttactattgg ttgtgtagat gatatccgaa gcatagataa gcttcttttc
     5281 ttcagctttc atctccttct ttggatcttc cgtaaatggt acaccaatgg tcaatcctag
     5341 aaaacgataa acttgaccca tttcctcggc atcacgcttt gataaatact cattaggagt
     5401 cacaaccatc actccttctc ctgaaaaagc gttcaaatag acaggcatgg tagctgtcaa
     5461 ggtcttacct tcccccgtat tcatctcagc aacatttcca tagtgcatga caatagctcc
     5521 catgacttga acatcataag gaaacatccc taaaatccgc ttatctgctt cacgcaccac
     5581 agcaaaagct tcaaccaaaa tatcgtctag actttctccc tcggaaagac gctgacgaaa
     5641 ctctactgtt ttagctacta attcttgatc cgaaagagag gacatcttgc ctttcagggc
     5701 attaatctgc tttaaaatct ttttcacttt cctaagctgg aaatcttgac ctaaacgtct
     5761 aaacacttct gatctccttg atagaaaaat gataaaaagt taaggactca aatccagcac
     5821 ttaaaagaga aactcgataa gtataactat cttctggata agtaaatatg aaatctttca
     5881 ctttttctac ctttttttct aaaatatcac catagcggtc aaaaaaagtt atcttggtat
     5941 aaactccatt ttcaggctga cagtcaaaaa ccagtgatag ttgatactcc tgctttcttt
     6001 ttaataaggg aagctgaggt tgggttcgtg cagcctggta attgacacta gaaaaccaag
     6061 tctttagaat ctcaccagat ggcaatagag gattatacag acgaacactt ttgtcagggt
     6121 aataagatac agttgttcca tacatataag tcccaccaac ttctccccaa tgaatagact
     6181 gtctctgtgt aataatcatc ttttctccct tccaaaacca gtctctaaga ccattctata
     6241 aaagtgcaaa aaccaagaaa tattggtatc tgtatcatca ttgtgtcgtc cagcagttcc
     6301 tttagataaa atcttcgcac ctgtattaca cagataagac actaattgtt cataggcttg
     6361 tggatccatt tcttcatctt tcatatagga taaaccaaag gttgtctttg aaaaatttgc
     6421 ttgtttaaaa attttccaaa aacgctgatc caactccgtc atatcttgag aacttgtccc
     6481 acctgtatga tgaattaaac aatcaaaagc taagttagag acacccggtg cgtccaaacg
     6541 tccacgacta gctatagttc ctaaattagc caagggctta ccaactatga tggcatgggg
     6601 ttcaaaagaa gcaccataat agagagccgg aaacgttccc atagaaagac ctgataaaat
     6661 caaatcctta tgatcaaagc ctaaataatc aagatagtga gtgatcgtat cctttacttt
     6721 tccctctagc tcatccgttc cgagataaaa agctcccccc tctaaacgtg gatcagaaaa
     6781 taaaatgaag ggacatccaa gagttttcat cataaagtaa ccctcgaatc cttctgcagg
     6841 tcgataacct gcaaaataga cagtcaaagg cggtttaaaa tcacctggat ggaagaaata
     6901 gtttatttca tcacgcttgg aatcatgtag gatatttcca ccaagtacaa acttaccaaa
     6961 ttgttttcga ctccatcgtt ggtgaagatt acctagtttg atagttccac gacctcttgc
     7021 ttcaacagat atcgagagaa agtagtctcc gtctcgacta tccataaaaa gtgcttgctc
     7081 caagtctttt tcagatacta ttacttcttc aaagaaatca tcaacagacc cactccacat
     7141 tttacggatt actaaacgaa agtcacaatt gccttccttc tcatattcaa gccacaactc
     7201 aatagggagt gttttttgca ccataatatt ataagcccaa taggcaagct gggcaaaatc
     7261 ttgcccaaac tcaccttcca aactgacata atccaatcct tgataagaaa tagctcctgt
     7321 aaaatttgga ttgacttgta tcgtcggagg aaagagttta tcaccatacc caccgccaaa
     7381 aagagattta cttaaatcat tgattagttg ttggggactt aaaaaatcaa ttgcttgtgc
     7441 acatcgtttt tttagaaaat ccacaacctc tctatcattt atttgtagat tctggttata
     7501 aaataaagta taaggttcaa aatattttac ataggaaaat aagtctctgg aatactgacc
     7561 atcttctatt aaaactgcat gaaaacggtt aatatcctct ttttcaatta tttcacgaag
     7621 ggcttttgat gagtttgggt aaaaataata ccaactcata ttttcaggta tgtcatagtg
     7681 attttcccaa ttggcaagtc ctatctgtaa aatattaagt tcctttgaca tttttaacct
     7741 cctctatcca tttctctaat tttccaagaa attgttgtcc tgtgtgctcc ttaatcttat
     7801 caattgaata tatcaaggac tcattccact ccttcaacct atctgtgtaa taatgtgcag
     7861 ccttagaaaa ttctgtaaca tctgctaaca aataaccatt ttttaaatgt tcaacataga
     7921 cggtttcaac taggttgatt tgaggaattc ctgcactaat cccagcaatc tgtgtgtaga
     7981 gatgaggatg tctatttaaa tccacaatca aacggacaaa ttctagtgtt tttatcaaat
     8041 ctaactcatc attcaagtta acaaactgta agcgtaagtc ctggtgttga ttttcttcca
     8101 gaggattttc tgcatcacca taatcaatcg cctttcccag attttcggat tgaatgtttt
     8161 cttggatgaa agactcaaca atcccttcaa cctcattcat ttgctcctga ctagcagcaa
     8221 aggctccaaa aatcacctca gtatccttat tttcagcgac aaaggacaag acttgaagca
     8281 gagcttgatt atcaatcccc tgctcaaaat ccagttgaaa ataaatgatg gattctttct
     8341 tagtttggct tcgtcccaat cgtaatcggg tgtcaaaaga tgataaatga taacattgat
     8401 gcattcgttc aggatacaac tcctgcaaca atcgtaaact atcctctcta tccaccaaaa
     8461 tcaaatccga tttttcaaaa gttacatcta aatccctaaa ggtatcttga ggattacgtc
     8521 caatgaacag actcaacagt ttaggattag tactaggtaa acgatctagt accaactggt
     8581 catgatgaga atgagaagga agcataaaaa tatcctgatc cttcacatac gtttgcagat
     8641 aattctcaaa aaattctgcc accagatctc ccatattttg ataggtaagc attttaaagc
     8701 gataacccaa aattggattg acttctacct gtcctccttc ttttaaacgc tctctgaatt
     8761 gccactctcc cttgaggttc agatattctt gataggctgc ttgcccatct tcaaaaaaga
     8821 taatgctcga tacaaaccca cgatcatcca cgatatggtg catatgtatt tgaccatttg
     8881 gttcaaaata ctgtatatca ctgatgaagc cttcaactcc atgttcaacc ttagcaaatt
     8941 tcttcccatt tttttgaacg ataatcgtaa agggactata aataaattca cagtcatcat
     9001 cccactcaat atctctaatg cttaaaactt gggtatggag attatgaaaa tcttgcataa
     9061 catcaaaaac ggaataagta tccatttcta acacaccatg tctatataaa aaataacgta
     9121 gatgaggctg gtaagctaat actaatagac gagaatctat atcttgctct tgaaagagcc
     9181 gaatctggtg aaaggtatca tcaaactcta gacgaaaatg agaaaaatac catggagtga
     9241 tatctgcatg ccatgttctt tctgacccat accaagctgg aataaaataa tacataaaag
     9301 tctcctaaat caaaggtcta taggtctcat ttagccttaa agccttgacc tcgtctctaa
     9361 tcgtaaaaat catgcccccg aacatcataa ataagcctgg aatcattgcc aatcgtaata
     9421 acttttcatc aaacaaaaca aaaagcatgg gaccacctgc cataatcaca ttaaagagac
     9481 cacctatgac tgagaaacga aggaccaatc gattaataaa tcgactagta tccgcacctg
     9541 gataaatacc ataaatgtat tctccagatt ttttcatacg gtctgcaatc tcttctccat
     9601 tcatcgtaac aaaagcaaaa atgatactaa ataaaaataa gaccgaaata taaacataga
     9661 cccacaaagg ctttccaacc ataaattcct ttgataaagc cgctaaccct gaatgattag
     9721 ggaaaataaa tcccaacaag atgaacaaat aagctggtac actaagaaaa ctcatcacat
     9781 acatataagg catcccacct gcaggattca acatgatttc gagataagaa tagcgtttaa
     9841 atcgattgtg taagccaatt ttattaacag gaaccaaata gcgagctcga tacataaggg
     9901 caagtaaata agaaaagaca agggttaata aagcaagtaa cacaataatc cctgttggaa
     9961 tgtgtactgt ctgaaatgtt tccaaaacat cctgaggaat atttaaaacc atactggata
    10021 ggaggattac aatagaacct ccaatcccca tactcgcatt taaatctgac aaccaaacaa
    10081 gaaaaaatgt tcctgctatc agcaatattg tattcattag aacaaccaat attgcagaat
    10141 aggaggattg tactggcagt ctcaagctaa ctgccaagga ttgaatcaca gcaattagca
    10201 aggtcaggta cattttacgg cgatcttgta tttctataga cgtagatgtt aaacccaacc
    10261 gtttagaaaa agaaaacatc tgccataaaa tcatggcgga catccaaggg gataatccaa
    10321 cagaaaaaat tgataaactt cttagattcc cacctgttag ggcggctgag aaggctagat
    10381 aggctgttga acctcctaaa aaatctttag tatttaggtc aacaaaaggg agaataatac
    10441 gacttcccaa tacatagata aacagtagaa atgaggtaaa tagtcctttt tttactgcta
    10501 ttgacgaata aatcttagtc ataaaacact cccctcacct tcattgtttg tgatatggta
    10561 aataacaatt cttttatttc aacttttacc atttctttgt tctgcatcaa aaaacaactt
    10621 taatctgaaa caaatatctt tatattcctc agtatctgtt aattgatggt attctaaagt
    10681 ttgcttataa tcttttaaaa gattgacaaa tcgaatacgc agaacggaga catccaaacc
    10741 agccaagaca caatccgata ttttcttaga aaacacttca acaatatctc ttgcccagga
    10801 aagatcaaat tccgttctca tgatgcttcc tgaacgtctt ctatacagat agagattatc
    10861 gtttataaag acgattttag aggctaaaag ataaaagcga tgcatagttg cttcatcatc
    10921 aaagcggcga ccatttgaaa agtgaacttc attgaataat tcttttttaa tcaacttaaa
    10981 tgtcggcaat ataaaggccg agctattgaa tttccaatct cctgcttgac ggttcataat
    11041 ttcttgtata gctaattctt cgacgcaaaa atcttgccct gttacataaa aataaaaatt
    11101 cccgtcactt tcgtcataat tatagtaatt ccctataaca acatctgcat ccttttcttg
    11161 gacttgctga taaagagttt caatcatatt ttcttttacg atatcatctg aatcaacaat
    11221 cgtgatgtac tctcccttag ctacactaat ccccatattt ttagcttgtg ctgccccccc
    11281 ttattttctt gatgcaatac tcgcacacga ttatcttgca tcataaaagc atcacaaatt
    11341 tccccagaat tatccgtaga accgtcatca actaaaataa tttctatatt ttgataggtt
    11401 tgcttcagaa tatgctcgac acactccact aaatattttc ccgtattgta tatcggtacc
    11461 acaacactaa ttaattcact cattactcta tctctatatt cttcattcta tccactaaat
    11521 catctggtct ctccacagaa aaaataccgt catagagtcc atcatccata cttttacgtg
    11581 tgatatcaaa agcgaagatt ttcttgccac tttcttgtgc ccttgtaact acattaaaaa
    11641 cctctccacc tgtattaata tctaaataaa ctatagaatc gtccaatagc caatcaattc
    11701 tactatgtaa tacattttga tatactgtta cattcgtata ctgtgataga gaggtcagct
    11761 cctcagaaca atcacacggt gcagccaaat gaaaatgcca atcaggtaac cgttgtacta
    11821 aatactctaa atgttttata tctgcactcc atgtcacaag catcacttgt tttttagact
    11881 gattgcttct ttcaaaataa tttaaatcgg aacgttgaaa agcaatctct gaccaatcca
    11941 aatctctata aacccaccat aattcacgta gtctagatat actatatgta ttccaaggtt
    12001 tatcatgact agcatagtga acaattgtag gtggattgtc atctagacgt tcacattctt
    12061 gagcaaggtg ataaatatca atacccacca tataattata tgttttatct aaggctagcc
    12121 aattatcctc aaaataaata tttaaaatac tctgatcccc aagatgaaca acttgattct
    12181 gctcggccgc taattccaat aaactattga caatagaatg ttctttccac tttgcaacat
    12241 ccattagtaa cataccagta ttaaatccag attttcgtcc ttcataggca tagacatcat
    12301 caacagcacc aattgaatat cctttgagat ctatctcaaa caaagtagct agttccccag
    12361 ttacaatgat atcggaatcc agatacaata ccctatcaga ttcaaccact tctgtcgcaa
    12421 aaaatctaaa gtaagaagca taatttatat gaggtcctgt tttatagctt tcaaaaagtt
    12481 ctttatcaat gtgtacactg cgaattgtag aattcacaac ttccattttt ctccccaata
    12541 atttaaacca ttcaggagct atatcactgt tgagaatata aaaatcaaca tctctattgt
    12601 gatacaatac agactttata gtcgtctcta aaggaataag ataggcatta tctgccgcta
    12661 atactattga ttttctcata ctgttctcta cctcatgtaa gatctgattg ctgcaaccat
    12721 ttttttcgga atggagcttg gatagatacc ttcatatgaa atctcagaaa gattttcaga
    12781 gcaagtattg tcgaaagcta tcattggttt tttgtactta agcacaaact catatgcatc
    12841 ttctaatttt cgatcatgat tcaaatccaa atacaaatct gaagctatta ctatttgttc
    12901 caataacatt ggtaaggaat ttggatatac ggtaacatta ggaaaacgcg ttaaagctag
    12961 tagttcattt gccatatttg tataagcaac aatatgaaaa tgaacttctg gcaattgctc
    13021 cgccaaataa tcgattttct cgacacacca agaattcgtt aaattaacac attgcaactt
    13081 aaatatttgt gatttactag ggtacttcac actctttgaa aaccattcat ttaaaataac
    13141 agaccaatcc atcaaagagt attcccacca aacttctctt agacgtccaa cagaaaattg
    13201 attccaaggc ttatcctgag aaatatagtg taaaattagt ggcagtggtt cgagcggaat
    13261 atcaaaaatg aattgatgtt taaaggttgc cgccccataa tcatatccta tttgaaaatt
    13321 atattgatct tcaagggaac tatattgatc tttaaacaac atattcaaaa ttgactggtc
    13381 tccttcttcc acattctcat gttctttttc tgttaagtca atcaattttt gtcgaatagt
    13441 ttcagatccc cattttttgt tgttaatcaa gagaacacca gcattgaagc cgactcctgc
    13501 tccaaagcaa gaacgagctg ctgccaaata attttcacct aagtctaatt caaacaaatc
    13561 ggtcaaatca ccagtcacaa tcaaatcact atctagatag agaactttat cttctgttac
    13621 aaaatctgga ataaaatagc gtgcaaatgt catatgattg atatgaggta atttattaga
    13681 ccaattcatt tgaaactgtg agccaattaa cttgcagtca atcaagtcgc cccccatctc
    13741 ttgtaaatat attcttattt gactaaacca ttcctgagga atgtcctgat ttagcagata
    13801 aattttcaaa tgactattgt gtctacagag tgacttcatc gccgtttcga tttgtcgaat
    13861 ataagcgtaa tcacctgcaa atactacagc gcgtttggta tttctcattt taaatcctta
    13921 tcatttattt taaatagtct agtttaatat tcgtgcaaca atctgactgt tataggtttc
    13981 atcatcaata aattcaatat cttggtcgaa attatgttct gcagtatgtt tatgagaaaa
    14041 tttaacctta gttttagctc ccattttcat ccattcatac tctgagtcaa tatggcctac
    14101 atccaaaact tgatagccca tctggcatag attataactc agaacttttg ctgtaggtcc
    14161 aagcatacat aaaatcaagc gaccaccagc atacttttca atttcttgtt caagttcatg
    14221 aactctagaa aaggcactat gagaaggaca gataattcgc ttaatagaat ttgcctcatc
    14281 gaataaatca tttccgacac ctgaacgaga agtcgcacct tcgactatca gtaagtcacg
    14341 gttttcccaa atgcttttca atttttcaaa ttgagcttta gcttgactct tgtcttcaaa
    14401 atcgatataa gggcgagata caaaggttga gccataccat gaatcactgc ataactctct
    14461 gtaaaaatcc atataatgat ccatgtgatc tttccaaaat ggaatcgccc acgatgtaaa
    14521 cctaaaacga tctgtaaaag catcaggaag gcacactact aaatcttctc gactttcttg
    14581 gccgataatg tccctcatga ttgaaaccaa ctcttcatca taatcctggt agggaattga
    14641 atgccctgca agcatattga tttccccatc tccaaaacga actaaagaag agttgtgttc
    14701 aataatataa tccaaggttt catctatccc tttcacctgg attatatcaa gcggtttttc
    14761 tagcatatcc ttagttatca atttttgcaa atcagccaac attcgttcag gatgttcact
    14821 tgaatacaac ctttggccat tattcccatt ttgagattta taaaaggcta gtataggttt
    14881 agatagatga gccatttctg caacgatatc ggatgttgaa gtcagtaggt tgatatctaa
    14941 ataagcatcc atcttctcct tcaactcgtc tagcttatct ctagaaattg ccggatagag
    15001 ctgaatatta ggataacgat tagacaaatc atataatttt ggccccatag ctgtccaagc
    15061 agcaatttga aaaacaacat ttggcaactg tccagccaag taatccaatt gttcgatata
    15121 ctgagaagcc gtaaacgtta aacaagttaa acttggaagt tgtcgaaaac aggtaagatg
    15181 tccgtttttc ttaaaatctt taatcgtatc aatcataaag gaaactttcc cattttcgaa
    15241 aacgatttgt cctaactctc cgtgctgagt cgattgaaaa gctagtagcg ttttttcttg
    15301 ttcttgcaaa gattttaaaa agttctcatc tgcactacca tgattgatat ccaaatatag
    15361 atttgttgac tttttcagct tatctaagac cggtggaaca atttgtggat gcaatctcac
    15421 attattatat acagctaatt tttttaattt atctcccata tccgtccaag ctgcgatatg
    15481 aaataccacc tcaggtagag ctgtaactag ctcttctatt ccttctaaat cttgggaatt
    15541 cgttaaggtt aagcaagaaa attccttatc tagaggcgat attagttcaa attctcccat
    15601 atggtgttgt aaaatctgac tccactccaa atcatggaat tcccaccata aatcacgata
    15661 acgattggct gtcaaggtag tccagggttt tctgtaggtc gtaaaatgaa tcaccactgg
    15721 tttatcatta aaagccagat gttcctgcca attgttatac aaagccacaa tatcatgccc
    15781 tacttgtaaa ttataagctc gacctagttc taaccaatca tcttgcaaga cctgattaaa
    15841 aatcgtttga tcaccattaa aatgctcgaa acggccttct tcaacttcct tcattgtaac
    15901 aatgctctgt tcaattagtc gttcttttaa tttctcttga cgccattttt tattgttgat
    15961 taataaaaca cctgcattaa atgtaattcc atctgtatct ttaactgctg cgagtgattt
    16021 ttcttctaaa caaatactaa ataatttctc taaagaagta tttacaatca aatcactgtc
    16081 taaatataaa accttatctt cttggatata atctgcaata aaatatctag cataagtaat
    16141 gctactaatg tgatcttgct tttcccaatc ttgaaacaca gtttgttcag gtagtttaac
    16201 atcgataatc tcactaccta acatgcgagc tatttttcgt ggtttgcgaa accaatctgg
    16261 catgatatct tgattcaaaa tataaatctt aacatctcga ttgtggtata aaatagattt
    16321 tatcgttgtt tctaactgcc tggtgtaatt gcgatcccct gctagtacaa ttgttttgtt
    16381 catttcattc ccttaatcaa ttcccaagtg atagattgca tctaccaata actttttagt
    16441 gaaatagccc tcttttaaca aatagctaaa ggtcttgata cgattcgtca tttcttgata
    16501 ttcttgtaga ttcattttat caactatctc atgaacctct tccagactat ccgccataaa
    16561 gcccaagcct tgatctacta taaatttagc agttgacaag ctacttggta caatgactgg
    16621 aatgcccgct gttagatagg tactcacctt atgagatata ttcaaggtat agtattggtt
    16681 actttctccc tcattttgat gggttcccca gacaaggcca aatccaccct ttgataattc
    16741 tagcaacaat tcctcatctt ttttccatcc ttcgatgctg agacttctag cactagaact
    16801 agcttcccct ttatttgaaa atactctcaa aggcgtatct tgagaccaat tttgtaagtc
    16861 tggaaaacgc tctaaacttc cagcaaaaaa aagttctttt ttaaaagcag gggtgtataa
    16921 ggataaatca tgaggatgat cccacatccc ttgaacaaga atcttcttag tcgtcaatcc
    16981 ttcttccatc aggcgtgttt tcattctctc tgacggcact atcaaaacat ctgatagatt
    17041 atacatatac agataatctt tcatgagata atagttacta tcaaacatga ggggaacaac
    17101 atcatggata aagcaaataa ttttcacctg catatccttt agcttatcaa acaagagacg
    17161 atcaaattca aaaccattcc aggttggaga ctgaaagact aaaatatccc caatagagat
    17221 actggccata ataccatcca gacgcttatt catttcagaa ggactatctg aagcaatgtt
    17281 gtaaaaataa ataccaacct ctctaaatcc cagttgacta gctatctttt gaacagcatt
    17341 ttgagctaag ataaccgtac tatcaccagc catgccgtat aaatttgtta aatgtagttt
    17401 catattacgc tatctcattt tttattacta tctacggaag taaagacaat ttttccatca
    17461 ttcttctgta aatctctgtg aactgcatat tttgttcttc taattttgct atcactcttt
    17521 gtaatctatt tttataaatc aaaatatgat tggtcaagtc atagtctgaa cttgctaaaa
    17581 tagctatctt ttcttcccta gccccaattt catcatacat acgcttttca ctccatgtat
    17641 tcgataaagt atcagataaa ccaacacggt accagtaaac acaacgattc aaatagacta
    17701 tcctcgaagc tcttagaagt accttccatg tccagtaagt atcctctgca atttttccta
    17761 taggaaatgg taaatcttgt agtaactctc tcttgaataa cttcaagaca gctacagtcc
    17821 agttcccatt tctgacttct tcgacacctt ccctatccat aattgcttta ccttctatca
    17881 cttctagaga atcatctgga tccgtaacat aagtcatata cacatagcgt gtttcatcat
    17941 aagaattata acgcccgata ctaatatctg cgttttcctt tttcaaagca ccatataatc
    18001 ggtctaaagc atcatgttcc aaccaatcat cagagtctac aaaagtaatg tacgcccccc
    18061 ccccgaacat tcaataccta ggttacgagc tgatgaaaga ccgccgtttg ctttctcaaa
    18121 atatttgaaa cgagaatctt tctctacaaa ttcttcacat attttggatg aatgatctgg
    18181 agagccatca ttgattaata aacactcaaa attttgatac gtctgattct gaatgctatc
    18241 caaacacatt cgcaaataat tctcaacgtt gtagattgga actacaatac taattagttc
    18301 gtctgccata aacttattct cctataaatt gtctaatagc ctctaccatt ttgtttggtt
    18361 cggttgaaga aaatatatta gtttgattgc ctatatcatg actagtattt tcaaaggtaa
    18421 aaattggttt agatagttgt tgaacaacgg atacaatatt atccacttct ttataatgat
    18481 taatatctaa ataaaaatct aaattatcca aaatttttcg ataatcaaat ggagaaaaac
    18541 aaggataaat cgtaacattg ctatatctta ataaagcaac gacactagac gcaaaatatg
    18601 tatgtgctag tatagaaaaa tgtacctctg gtaaattttc tatcaaatat tctacatgct
    18661 ccatctcaca actagctgta aaaattgctg tatgagcttt aggactgtat acaagttctt
    18721 cgaaactacg actaataatg tcttttctca ataaaagatc agaccattct aacagattat
    18781 aaaaccacca aacttctcta aagcgattat tggaaattat ttcccaaggt tttaccccag
    18841 tataatgtat aacactaggt aaatctccat ttttcaactc agaaatctca taccatttat
    18901 gatttccttc tatgtgtgcg acgctatcca tccccaccat aaaattaaaa tttcggtcta
    18961 atcttttcca tctatcatgg aataacatat ttaaaattcc ttgatctcca tatgctgttt
    19021 catgatattg attggtcagt tctaacagtt tcgaacaagc atcttcatct ctccaagtat
    19081 ctacattaac taataacatt ccggagttaa aattggtaga aggaacatca ccaaaagaat
    19141 cttctactgc tgccaaggca taaccatcta gttctatatc aaataaatag tctaaacttc
    19201 ctgtaacaat gatgtcagaa tctaggtata aagcacgact ttctttgaca aaattaggta
    19261 taaaataacg aaagaaagtg gcataactta aattctttaa cggtaaatga aactttttaa
    19321 gaacatgatc tacaatcttt acattaacga tttcagattg tatagtcttc aatcgcttat
    19381 tcatcatcaa aaaccactct gacgcaatat catcattaaa tacataaaat tttaaacaat
    19441 tattataaca acaaatagat ttaattgcag tttctaattt atctacataa tcattatctg
    19501 cattaaaaac tatagatttt gtattcatta acataatttc cttataataa atttcgtatc
    19561 aaacagctat aatatagaat cattttcacc taaaaagata attaatcaca ccctaatatt
    19621 gtactgaccc caaaaagtta gattttttct gtctaacttt tggggtgcag ttcatattaa
    19681 atatctttag gtctatttat attttataaa gcactctgat gtttgaacaa ctttcatata
    19741 tgatgaacgg agaaatttta taaaaaataa tccaactctt caatctgatc actaaccgtc
    19801 aaaatcagag catctcctac taaatgattt ttcttcattt gcttatttct ctcaattttt
    19861 caatcattgc ttgaacttgg tcaacagcat atgcctcctg acctacttca taggttttag
    19921 tattttcaaa ggataagata ggcttgccaa gattagcaaa ttgatcgaga atttcttctg
    19981 tcttttcgcc atgattaata tctaaaagta cttgactggt ttctaccaat tcattatcga
    20041 catctaccaa atagtgaatt ccgttaaata tagtcacgtt tggataaatt gtcatctgag
    20101 ccaatcgatc actaactatt actctagctg ctatcttaaa ctgaatatca ggcaaggatt
    20161 gaaccaatgt ctcaatttgt tcaatatggt ctgaggcagt atagattaga caagtgaaag
    20221 gttcctttat tggatagatg tgagatcttt gtaatggatg taaatgatgg ttttgtccca
    20281 attctgtcca ttcaagccca tgatagtacc accaaacttc acgataggtt tgggccgcca
    20341 aatctttcca cggtttccga tgagaaagat agtgaataat agcaggataa tcctgaccct
    20401 caggcaattg ataatcagca aactgtttat gaatgacaat atgattataa tcaaagtcca
    20461 attccaacca tttatgttca aaaagcatat tcaagatgct ctgatctgcc tgatccacct
    20521 tatcatgcca ttcattggtt acatcaatta atttttgggt catattctct tttttccaaa
    20581 aagcattgtt taccaagaga acaccggcat taaagatttc ttgaccaaaa taagctctgc
    20641 ccccaaaatc tctaacagca gccaaaggat aatcttgtaa gtctgtagca aacaagtcat
    20701 ccagattttt cgttacaact agatcacagt ccaagtagag ggccttgtct tcttgcacga
    20761 aatcagctat gaaatagcgt aaaaagactg tgtaactaat atccgattta taacatgaaa
    20821 tttgctcaga agttacccga caattaataa tttctgagtc aaacttctct aagcgcttat
    20881 ttaattgctt aatccattca tttggaaaat cgctatgaat cagataaaaa cgaatcgaac
    20941 gattatgata acaaatagac ttgattgtcg ttaaaacttg gtctacatag ccatagtttg
    21001 ctgcgaggac aatggctttc ttttcacttt cctcttgtct cgatagctga tttaaaagcc
    21061 tttgtttcat ttcaaactct ttatacgttg ctgtgtcaga taaaccacta gcttgaccgt
    21121 tggcgagact gacttccaac atctgacgat aaactgccaa gtgtttctct agaggataac
    21181 ccatattagc tagtagcgta atacgttcag acatagcatc aactaaagcg tgcatccact
    21241 tttctgtcca aactcttgat aaactacctt ttctaatccg ataagcataa agacttttat
    21301 ttaaataaat taccttttct gataataaat ataccttttg attgaggtaa ccatcttctc
    21361 ctaatttacc tatgtcaaag cgcaactgct caaacaatct tgccttatag agtttacccc
    21421 aagcagatat caaagcaaaa ctcttcattt cttgagtttc atacaagttc tcaaagatag
    21481 aaacattatc atatactttc tcataatagg agtctcccaa tatatgaaag tagaacattc
    21541 cttcactttc gttgaaagaa taataattac caactgcaat atcagcctga tactctacta
    21601 tttttttata tagagtttct acataatctt gctcaatcca atcatccgag tccacaaagg
    21661 tcacataatt tccggacata ttattcagac cggtgtttcg tgcggcagaa agaccagcat
    21721 tttcttgttc tatatagaga attcggtgat ccatttctga aaattcttta caaatttcac
    21781 ctgaagcatc cgtagaacca tcattaacga caacaatctc aatattttta tatgtttgag
    21841 taataatact atctaggcac ttcctcagat agttttccac attgtatact ggtacaatga
    21901 ctgttatttt atcatccact agcaattttc tccttgattt atgtgcgcga gttagtttag
    21961 cctaacttta caggttgtct tactcttctt catcacgttt acggcgtttc gcaaccaatc
    22021 caatacctgt aacagctgct agaactccaa gtaacacaga tccaattgac gactcagtac
    22081 ctgtattagg caattctttt tgggatttac ctgtcgatcc agaagtattt ccaacttgtg
    22141 agttcgaatg gtttgctgaa tttgaaactg atgtacttgc tgaagctgac gcacttgttg
    22201 atgctgaagc tgaagcactg gtgcttgctg aggccgaggc actggtgctt gctgaagccg
    22261 acgcactcgt tgatgctgaa gctgacgcac ttgttgaggc cgaagctgaa gcacttgttg
    22321 atgctgaggc tgacgcactg gtacttgctg aagctgaggc actggtgctt gctgaggctg
    22381 aggcgctggt acttgctgag gctgacgcac tggtgcttgc tgaagctgag gcgctggtac
    22441 ttgctgaagc cgatgcactc gttgatgctg atgctgatgc actagtactt gctgaggctg
    22501 acgcactcgt tgatgccgat tcagacgctg agatacttgc tgaggctgag gcactggttg
    22561 atgctgaagc cgacgcactg gtgcttgctg aagccgacgc actggtgctt gctgagactg
    22621 atgcactggt tgacgctgaa gccgatgcac tcgttgatgc tgatgctgat gcactcgttg
    22681 atgccgattc agacgctgag atacttgctg aggctgatgc actggttgat gctgatgctg
    22741 atgcactagt acttgctgag gctgacgcac tcgttgatgc tgatgctgag gcacttgttg
    22801 atgccgaagc cgaagcactg gttgatgctg aagctgacgc acttgtgctt gctgaagctg
    22861 acgcactggt acttgctgaa gccgaggcac tggttgacgc tgaggctgaa gcacttgttg
    22921 aggctgaagc tgatgcactg gttgatgctg aggctgaagc acttgtactt gctgaggccg
    22981 aggcgct
//
LOCUS       DBDESG010000032        22880 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.032, whole genome shotgun sequence.
ACCESSION   DBDESG010000032 DBDESG010000000
VERSION     DBDESG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.032"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..188)
                     /locus_tag="ACKB38_001234"
                     /pseudo
     CDS             complement(1..188)
                     /locus_tag="ACKB38_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(250..699)
                     /locus_tag="ACKB38_001235"
     CDS             complement(250..699)
                     /locus_tag="ACKB38_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGL7980002.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(701..1459)
                     /locus_tag="ACKB38_001236"
     CDS             complement(701..1459)
                     /locus_tag="ACKB38_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGL7980003.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1574..3553)
                     /gene="stkP"
                     /locus_tag="ACKB38_001237"
     CDS             complement(1574..3553)
                     /gene="stkP"
                     /locus_tag="ACKB38_001237"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGL7980004.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMHLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3550..4290)
                     /locus_tag="ACKB38_001238"
     CDS             complement(3550..4290)
                     /locus_tag="ACKB38_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGL7980005.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4305..5618)
                     /gene="rsmB"
                     /locus_tag="ACKB38_001239"
     CDS             complement(4305..5618)
                     /gene="rsmB"
                     /locus_tag="ACKB38_001239"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGL7980006.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5611..6546)
                     /gene="fmt"
                     /locus_tag="ACKB38_001240"
     CDS             complement(5611..6546)
                     /gene="fmt"
                     /locus_tag="ACKB38_001240"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGL7980007.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6559..8955)
                     /locus_tag="ACKB38_001241"
     CDS             complement(6559..8955)
                     /locus_tag="ACKB38_001241"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGL7980008.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRSGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGSTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9021..9335)
                     /gene="rpoZ"
                     /locus_tag="ACKB38_001242"
     CDS             complement(9021..9335)
                     /gene="rpoZ"
                     /locus_tag="ACKB38_001242"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGL7980009.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9360..9986)
                     /gene="gmk"
                     /locus_tag="ACKB38_001243"
     CDS             complement(9360..9986)
                     /gene="gmk"
                     /locus_tag="ACKB38_001243"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGL7980010.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10116..11729)
                     /locus_tag="ACKB38_001244"
     CDS             complement(10116..11729)
                     /locus_tag="ACKB38_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGL7980011.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11920..12174)
                     /locus_tag="ACKB38_001245"
     CDS             complement(11920..12174)
                     /locus_tag="ACKB38_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HGL7980012.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12178..12432)
                     /locus_tag="ACKB38_001246"
     CDS             complement(12178..12432)
                     /locus_tag="ACKB38_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGL7980013.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12670..13767)
                     /locus_tag="ACKB38_001247"
     CDS             complement(12670..13767)
                     /locus_tag="ACKB38_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGL7980014.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13777..14517)
                     /locus_tag="ACKB38_001248"
     CDS             complement(13777..14517)
                     /locus_tag="ACKB38_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGL7980015.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14626..14979)
                     /gene="rsfS"
                     /locus_tag="ACKB38_001249"
     CDS             complement(14626..14979)
                     /gene="rsfS"
                     /locus_tag="ACKB38_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGL7980016.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYLVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(14992..15492)
                     /locus_tag="ACKB38_001250"
     CDS             complement(14992..15492)
                     /locus_tag="ACKB38_001250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGL7980017.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15493..16086)
                     /gene="yqeK"
                     /locus_tag="ACKB38_001251"
     CDS             complement(15493..16086)
                     /gene="yqeK"
                     /locus_tag="ACKB38_001251"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGL7980018.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLVLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(16086..16715)
                     /locus_tag="ACKB38_001252"
     CDS             complement(16086..16715)
                     /locus_tag="ACKB38_001252"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGL7980019.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16767..17078)
                     /gene="yhbY"
                     /locus_tag="ACKB38_001253"
     CDS             complement(16767..17078)
                     /gene="yhbY"
                     /locus_tag="ACKB38_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGL7980020.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17292..18398)
                     /gene="yqeH"
                     /locus_tag="ACKB38_001254"
     CDS             complement(17292..18398)
                     /gene="yqeH"
                     /locus_tag="ACKB38_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGL7980021.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18401..18928)
                     /locus_tag="ACKB38_001255"
     CDS             complement(18401..18928)
                     /locus_tag="ACKB38_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGL7980022.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(18943..19851)
                     /locus_tag="ACKB38_001256"
     CDS             complement(18943..19851)
                     /locus_tag="ACKB38_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGL7980023.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESETVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19908..20468)
                     /locus_tag="ACKB38_001257"
     CDS             complement(19908..20468)
                     /locus_tag="ACKB38_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGL7980024.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20607..21816
                     /gene="sstT"
                     /locus_tag="ACKB38_001258"
                     /pseudo
     CDS             20607..21816
                     /gene="sstT"
                     /locus_tag="ACKB38_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21884..22837
                     /locus_tag="ACKB38_001259"
     CDS             21884..22837
                     /locus_tag="ACKB38_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGL7980025.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6146 a         5398 c         4493 g         6843 t
ORIGIN      
        1 gggcgattga tttttacttc cgtcacatca atcattatcg tgtcctcaaa gctgagagga
       61 gttcttgaaa tcgtaacacc actttgaaca agagttactt caacccattg gctccgacgg
      121 attaagttgc tttcgtggat accaaaatca gccgcaattt cttcataagt gcggtattct
      181 cgcacatata gaaagcgtta tcaatttatt tatctcattt ttcagaaaat tcttttattt
      241 ctgtaaagtc tacgatactc gatgtgtttt tatataatga tagagtctga gaatcactgt
      301 tccgctagcc attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc
      361 cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc
      421 tggaaccagc ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat
      481 acttaaaatc tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac
      541 attggtcggt tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc
      601 aggtaagagc atagaccgtt gcacattgag tacgattaaa aaagtgataa tggcaagaaa
      661 acttgctact gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg
      721 cgacagaagt tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca
      781 taccagagtt tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg
      841 gtacaaacgg catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt
      901 agcgagccca aaactgggca attatcccaa agacaaaggc tccagcaaag gctgtcacaa
      961 agggaattcg gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca
     1021 ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac
     1081 taaaggtcgc agccagagtt acctgcaact tagtataggg aaggggtttg gcttgcaagg
     1141 ccgtcaattg cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa
     1201 tctggttcac atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc
     1261 gcgaaatatt ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc
     1321 aatccacaat cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt
     1381 ctgagccact tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta
     1441 attcttttga ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat
     1501 ccggactcaa aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat
     1561 tatccatctg cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga
     1621 ctctagtctt attgaggtct accttttcac ctgctctagg actttgttca acaaccatgc
     1681 cttctgcact acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa
     1741 tcccaacaat ttgaatcaaa ttgttcttag taaactccaa gctagaacca atgtaactcg
     1801 gcatggcaac acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca
     1861 catcataagt cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc
     1921 tggactcttc ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg
     1981 agattacttc tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt
     2041 tagctactgt caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta
     2101 gactttgctt caggaccgtt ccagcctcac tctcattcga ctcttcttcc tcaattttaa
     2161 tcaaattatc tggaactttt ttctctttta attccgcaat gacatcagag gatttccgac
     2221 cgacataatt actaatttgg aaagattgct tgcctgatga gacaaccaaa ttgattttcg
     2281 ttccttcttt tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca
     2341 ccttttcact agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg
     2401 ccttggcctc tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc
     2461 tggatagtat ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt
     2521 aacgcatctt aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag
     2581 cctggcttgg gtttttgatt gatttgtgtt ctgtttgcgc ttgaacctta ggaatagatg
     2641 tcaaggtact ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa
     2701 agattaactt actttcattt ctacgattgt aggacaagct actagacaag tccacataca
     2761 tctctgaaac cgagcggtag cgattggtca actttttagc agttgccttg ataataacat
     2821 tttctaaagc ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga
     2881 aatgctggag ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcagca
     2941 tctcatagaa aataatcccc atggcataga tatcactctg cacagtcgcc ttcgaaccac
     3001 gcgcctgctc tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac
     3061 ttgtctctgc aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg
     3121 tcaagaggat attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagt
     3181 gcatagccaa gagaatttgt cccatgatac ggactgcttc ttcattagaa agaggataat
     3241 gttccttgat atagcgtttg aggtccagtc cagccacata ctccatagct aggtactgtt
     3301 gaccgtcttc ctcgccaata tctgttatcc gaacgatatg aggatggtct agatctgcca
     3361 tagctctcgc ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc
     3421 tcagaacctt cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat
     3481 ccgccatacc tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc
     3541 cgatttggat cattctgcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc
     3601 taaacctcct gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat
     3661 atcactggtt acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact
     3721 attgagcaag agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc
     3781 atctttttgc ccaatagact gggtgataat atttttttgc ggatgagctt ctgcctcttc
     3841 tggtgtcaat tgaccagcct tgagcaattc attaaccaag gaatgatcgc tcgtcaactg
     3901 atggtattct tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc
     3961 ctgattatca ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc
     4021 ctgaccaagc tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg
     4081 cacttcattg actgtatcga tctgggtatc aacccaagct acacccaggt ctgtgaccgc
     4141 catttcacta gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat
     4201 ggtacgtcca gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg
     4261 accaacatct gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg
     4321 cgaaattgac tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag
     4381 ccgtctttca tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga
     4441 ctctctaaaa aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta
     4501 taagttatta taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga
     4561 atttcctgca aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg
     4621 cgcaaaagac cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg
     4681 tcaaaaaact catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact
     4741 cccagacgtt gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca
     4801 gtaacctgac ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca
     4861 caggcatcaa gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg
     4921 gactcatctt ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc
     4981 tgctccttaa ccagaccagt ggttgctaaa agggaattat tcgcctccaa caaggcttgg
     5041 atttcctctt ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg
     5101 ctttcaaaga tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc
     5161 caaactggga gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata
     5221 tctggccagc cttcacgcaa gatacggcga aggacagcgt tgaccaattt ttcactgcct
     5281 tttttacgga gtttggccaa ttccactgct tcattaacca cagcatgatc tggaatcttg
     5341 tccaaatagc ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac
     5401 tggtctctgt cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc
     5461 gttccataga ccagctcggt cactaagccc ttgtctgctg ccaaaagttg acttcccttt
     5521 agatgcttat ttaaggcgat atttgaatat gcttggttca caaaaacatc ctctagcact
     5581 gctagagcta aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa
     5641 tgtacgtcca actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg
     5701 cacttgtttg agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat
     5761 agagagaatc tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa
     5821 gcggtcgccc ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt
     5881 aaagagttga cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg
     5941 agagaaggta acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc
     6001 aggcagagtg tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc
     6061 aacattgtcc tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat
     6121 ttccttaacc atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata
     6181 atggataggc gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa
     6241 gtccatgcta tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc
     6301 atctgctcct agcttcataa gatcttccat ctctggactt ccagataatt tttcaggttg
     6361 gtagatagat agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt
     6421 tttacgacca acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc
     6481 tgtcaaaagt ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt
     6541 tgtcatatct tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag
     6601 ctcactattt tcccgttctt gagtcaaggc taaaacctgg ttgagggtcg accccagctc
     6661 atcttctaaa cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat
     6721 cggttttggc gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt
     6781 catgacttca taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc
     6841 aatcgtgaaa tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata
     6901 aaagccttcg taatcttgat ccttggcaaa tcgaatagca tagtgctgcg gattgtagga
     6961 ctgtatcaag acttgacctg ccttttcagc ccgacctgat cgacctgcca cctgagtcaa
     7021 gagctggaag gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt
     7081 tagaactccg actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc
     7141 aagtaaaata tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt
     7201 ctttcgagtc gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag
     7261 ctcatcataa gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt
     7321 aggacagacc tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt
     7381 atccatatgc aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc
     7441 ccgacacatg acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt
     7501 tttaaccaga cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt
     7561 ttgtccgata tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt
     7621 ggcacgttgg gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc
     7681 taagctcggc gttgcagatc caagtaccag agttgcttga ttatactgag cccgtaaaat
     7741 agctacctct ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc
     7801 ttcatcaata atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc
     7861 aacaacaact tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt
     7921 ggataatcct gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt
     7981 catctgagga gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag
     8041 ggcaccttgg ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag
     8101 tagaaaggga ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg
     8161 ttctggattt aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg
     8221 ttgaacttcc ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc
     8281 tcgcgagtag gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata
     8341 atctctcagt tccaactttt tcttggcacg tgtagaaatc tcaacacctt ctaattgagc
     8401 atggtcaacc tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc
     8461 cagaccaagc aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag
     8521 tgaagaaaag gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa
     8581 gccttccaga ggatagagaa tcttgtcata gctagaattc agaaaccctg gaagcatggc
     8641 cttgaggata gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag
     8701 ttgttctggc gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg
     8761 ctccatctct tctccatctg attgggactt caaaccaaga acaatccctt gaatcaggcg
     8821 attaccctta ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc
     8881 ctccggaatc ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat
     8941 cttagctagg gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat
     9001 aagattgagt ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc
     9061 tctttgattt tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc
     9121 actgcttcac gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct
     9181 tctaaagcgc gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct
     9241 tccaattcgt gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg
     9301 tcgagcaagg tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt
     9361 tatcgggtag ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca
     9421 cagaagtgtt ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg
     9481 ttgacaatcg cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt
     9541 cgttgggcaa tcacttctgc actatctgtt ccacgaccta ccaagcgatc ttgcaattca
     9601 tccaaatctg gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga
     9661 agagcaccct gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg
     9721 acataggtca gaggagttcc atagtagtta ccgacatatt ctgcgtattc caacatctgt
     9781 ccttgacgaa tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc
     9841 acttctccag gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca
     9901 gaactctcaa aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa
     9961 acgattagta agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat
    10021 ttctctagaa taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac
    10081 aaaaaagcag taatttttca actgctcttt cttatttatt tagcataatc tactgcacga
    10141 agctcgcgaa tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc
    10201 ttacgaactt tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc
    10261 atgatacgaa tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg
    10321 ttagcaattt cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga
    10381 gcacctggac gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc
    10441 tcagcttcaa catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg
    10501 tacttacggg ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg
    10561 gctttcccga tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt
    10621 tcgctcgcca tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt
    10681 ccatatgaag tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt
    10741 ggcgcaccaa tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct
    10801 tgacggtttt tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc
    10861 aacatttcca tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc
    10921 accacttctg gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga
    10981 atgttacgac cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact
    11041 gttgagtttg ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag
    11101 atgtccttgg ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga
    11161 ctggcaatct ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct
    11221 gcctgagaca gcgcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct
    11281 aattgctctt cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt
    11341 tcaagtgttt gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc
    11401 tctgtcaaac gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc
    11461 acttcttcac ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga
    11521 gacttgcttt cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca
    11581 cgtaaattag ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat
    11641 gatttcatct tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa
    11701 acagcaatcg caagcgacat gatttccatg tttttacctc attttattgt tattccgaat
    11761 gacatacatt cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat
    11821 atgttttgag gaatttggaa cacatttacc aaaataaact tgttgtttag aaatagtagt
    11881 ttagtagaga cttgagaaaa agcctacctt tcaatagact tagtaatgat ctttaaagga
    11941 caagaaagcc acgctatctc catccatcat ataaatcaag cgattttctg catcaatacg
    12001 ccgtgaccag gctccttggt aatcatattt gagtggttct ggtttaccta ttcctgtaaa
    12061 gggatcacgt tgaatatcct tgattagttt attgattctt tttaacgttt tcttatcctg
    12121 attttgccag tagcaataat ctgcccaggc atcttctgta aacttgagca gcatttctta
    12181 ctcctcaata acatggacct gagtacttcc agcacgaact tgagccattc ctcgcaaaac
    12241 cttatcagaa agttccttat tttgagcaat tctcagggtt tcttggatac tatcccactc
    12301 actctttgaa aggactacaa tgtcctcatc tggattttta ttgaccaccg tcaaaggctc
    12361 aaattcatca tttaccttct tcatgtagtc ctttaaatga tttcggaatg ttgagtaaag
    12421 gactgcttcc ataaccatac ctcgttttag ctcttttcca ctattataca cgaaaagaaa
    12481 gaaattgtca ggaacttgta caagattttc ttttctatct atttatactc aatgaaaatc
    12541 aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggcaagg cgacgttgac
    12601 gcgatttgaa tttgattttc gaagagtatt attcgtaaaa aatctcaaaa agcctacctt
    12661 tcggtagact tagtttgttt ctattctaat cggcactctt ccaaaatttt gctctgctat
    12721 acttggcttc cctagttggt aaatctggtc tgctttttgg gtcatagcat cccaaggttc
    12781 tttgccaatt cggctgacta gactgacctg acctttcaaa gacttgagat gttgcctacc
    12841 tttttcagta aatccaagga catgaatggc ttctggcaag tcactttctc tagcctgcat
    12901 caaaatatag gtcaataggc gtctgacacg cgccttggtg taacgtttgg tagtcactaa
    12961 ctcgaccaat tcttccacag actgggctgt tttaatagct tctttaatgc gcacagccat
    13021 ttcttgattg acctgataga tggtggttag gtcgggattt gacaagattt gatagcggag
    13081 caagggaaaa tagtcttccc agatcacctt actggcctgc tcaaataggg caacagaagg
    13141 cataaaacgt tctaagaaat cttggtcctt ctgatgttga cggagggctg tcgccgaggc
    13201 aaagtccaca tctttattca cagaatggta acctgccccc tgacgctgaa tcggatgcag
    13261 cttgatattc cgtcctgcaa ccgccttggc ataggccaga gcaagaacat gattgggggt
    13321 attacctgaa aaatcaagac cagcaaattc cttccacata gcttgggttt tctggggata
    13381 ggaaagggaa tcaggcagat tttccacaaa tttctccatc tcagcacctt tctctgtgta
    13441 taagtcagca attttctgat aatctcgaac ttcctctgtc ccaaaaacta gactatcaat
    13501 gcccaagcga tccaagatat ccatagctcc ttgaccaaag aaatcagctg cctgaacact
    13561 gactaaaaag ggcaattcca ctaccaagtc tgcgccattt tccagcgcca tctgggtcct
    13621 tgtccactta tccacaatag caggctctcc acgctgcatg aaattccctg acatggcaac
    13681 aattttcagt ccctctgcct gatccagcag gtatttatgg ccattatgaa aaggattgaa
    13741 ctccgcgata atacctgtta tggtcatgct aatctcctac ttctgcgcca caaaaaacca
    13801 acgggtgcta gtttctgttg gctccttatc ctcaaagtct gcatagagtt tgaaggactt
    13861 aaatccagcc tgttccagca agatatcata ggtcaagatc tcataggtcc tctcctcatg
    13921 tacttcatcg tggcgactaa aggaaccgtc agcctccttg ataaagaaag tcagctcatg
    13981 cacgatggag tgaggaactt cgtcctcata tgtatcccaa agcatggcaa aatcttccgc
    14041 attttcatgg taggaatagc cagggaatac ttcatctgtc tggtaggtcg agtgcacgtc
    14101 aaagatgaaa actccctctt cattcagcgc attgtacacg tccttaaaga cgtcccctac
    14161 ttccacctca tcctgcatat agcagataga gtccgaataa cacgtgacaa aatcgtattt
    14221 ccctgccttg gacaaattca gcatattacc ttctataaaa gcaatctttt gcttggctga
    14281 agttgctctc ttctccgcaa tcttcaacat atccgcactc aagtcaagtc cagtcacatc
    14341 aaaaccagcc tgagagaagc gcactgattg aattcctgtc ccacaagcca attccaagag
    14401 tttctttctc tccttggtct taggcaaatg acgcagagaa aaattcgtcc atttgtcgta
    14461 taaactatcg tccatgaccg catcatagac cgccgcaaag gtttcataag tcgccataat
    14521 catgatacta agagctccat ggtaaacacc actagccctt tacctttcta tcaatttttc
    14581 taaaataagc aaagaaaccc agttgactac aactgagttc atcttctaag caagagcttc
    14641 tgaaatatct actgaattcg cctcatgcca tagcttctct agattataat gggcacgcat
    14701 ttcttctgaa aagatatgca cgacaacagc accgaggtcc agtaagaccc agcctccagc
    14761 tgcatcgcct tcgacatggc tacctttaaa gcctgcttga gctacttttt cacggatatt
    14821 agctgcgata gcgtccaact gacggctatt cattgagcta gtgatgacca agtagtccgt
    14881 cacactagtc aaatcttgta catcaagtgc gaggatatcc tccgcacgtt tctcatcagc
    14941 cgctttcacg actagttcta gtaattcttt ttcgttcatt taatcctctt tttagaaaat
    15001 atgtttgtaa tctagaacat ctgcaaagcg ctcctcccaa atgtcctcat aaaattcatt
    15061 aatcgtttct gctggaatgt catccccatc aaaggttgtg acagcacctt ctggaacaat
    15121 aagctgatag ccatattcaa aggcaacctt gacagaggta tccacacaat attctgtctg
    15181 cataccacat aaaactaatt tttcaatccc ctgcttatcc aagtattctt ttaagccagt
    15241 ttctttgaaa atactgttat acttcttctg aaagaccttt tcagcaggtt ttcgatttaa
    15301 aagcgcagat aactgccaat cttctgatgt ttgagcttca gagttctcaa tatgttgaac
    15361 atagataatt tcaatattct tgcttctagc ctggttttgt aaataagaaa ttttttccaa
    15421 tagacttttt gtctggaacc cagtttctac taaaacattc tgaacatcaa tgattataaa
    15481 agccgtcttc atttagtcct ctttcaaata gtgcacaaag gcgttatagg tttcaagggt
    15541 ttggggatag atggggaatc cctgatgagc tagatactcc acggtacgag ctgtttcgta
    15601 ggccaccgcc ttattgagcg atagacttgc aatctcacgc gccacatcta ctccaggaaa
    15661 ggctcgattg tgctcgatat agtctgcgac gtagatgacc ttatctaggt ctgtcatttg
    15721 accagctccg actgtatgaa tttcaatggc tcgcagaatt tcagaatcat gcaaatccaa
    15781 atcttcctga attttgtaaa ttccgaccat accatgccag acattattgc cccagttttt
    15841 gaggtcaggg tctagctggt aacggtcaat caagactaga aattcctgat ctgacaactt
    15901 tttagcatag tcatgaagaa gacctgctag acttgctttc tcaacatcta caccaaagcg
    15961 ctgagctaat tccatagcgg cacgctccac acctagacaa tgggttagac gtttctcagg
    16021 tagaagctct gccatttttt ccaacaaggc ctcacgggag cagttgatat agtcttgata
    16081 ggacatcagt agagcccttc cttctcgatg tagtctagca ctggctgagg taggagaaag
    16141 ttgggtttcc gaccttgggc aaggaagtcc cgcaccatgc tggacgagat atccatgagc
    16201 ggtacgtcca cccagataac tggataggaa gtccctacct tgtagcgtgg acgctgaacc
    16261 cccacaaact gaaccatgtc aaccagttca tcaattcggt accacttagg cagatagtca
    16321 accatgtcgg caccgatgat aaagtaataa tccgtatctg gattcttctc tgtcaaaatc
    16381 ttcatggtgt cgtaggtgta ggaaataccc ttgcgctcca actcaatggt ttcaatgact
    16441 aggccgtcaa ttccctcaat tgccaactca agcatcttga gacgatggtg ttcagggatg
    16501 gtttcctttt tatcaacgtg aggaggttgg tattcaggca tgagaagaac ttgatccagt
    16561 cccaactgtt gccgtacttg atccgcaaca atgagatggg cattgtgaac agggttaaaa
    16621 ttccccccta aaatcccaac ttgtttgcgt tttttctcct tgatttctgg ctccaactct
    16681 accttggtaa agggagtcaa tagttcgatt gtcataggct agtccccttt atttctctat
    16741 aaaaacagtt atttggagtt tcggtcttag atttctttga ctttcttaga aatcttgcga
    16801 ttttctttct tgctagattg tttaaacaaa atcaagatgc gtcctatttt ttggactgta
    16861 tccacaccga tttcttcttc caaaatttca gctacttcgt ggatgttttc atctgtgttt
    16921 tgtaagagag taaccttgat taattcacgc gcatcaagag cttgacggac gctggttttg
    16981 atttggtcgt tgagtccatt tttcccgatt tggatgatag gtttgagggt gtgtgcctgg
    17041 ctgttgagga aggcacgttg ttttgatgtt aatgacataa ttttcttcct tgttttttga
    17101 tagttatttt aggtggtgag ggacggtcgg aactaatttt ccaaagattt catctttgaa
    17161 aaatggattt cctgacctca caattgagtc ttggtctcaa ttgtgcccct tgccaatcta
    17221 cagattggca atcacaccac agcaataact atcgctatat gagctcactt tgttcgctca
    17281 gcgattttgt tttaaataat tgcttttcgt gtgacgacgg cgacgccttc tggtgcccag
    17341 acggcgactt ttgctatgcc tgttacacga atccatccta ggcctgagat aactaggtct
    17401 gtcttatctt taatggtaaa gacatgctgg actagccttg ggaaatcttc tttttccttg
    17461 ctatttggtg gtgtcagaag agttcccagg tgcttatcgt agaaagcact agctccttca
    17521 agcttgctac gatggagttt gagttcatta tcaaagaaag cagtaaatcc ttgcttttct
    17581 cctgctataa agtcaaagcg tcccaaacca cctaaaaata gggtttgctc aggattaagc
    17641 tgataggtct taggcttgat ttccttttta ggactgacat acttgaggtt tttggccgtc
    17701 aagtagtgag ccatctggtg gcggtggata attcccggcg tatcgtaaat ataagatccg
    17761 tcgtcaagcg gaatctctat tttgtccaag gttgtccctg ggaagcgtga agtagtgatg
    17821 acattctgat cacccgtgat ttcttggata atagcattga ttagagttga ttttccaacg
    17881 ttggtcacac cgaccacata gacatcgcgg cccttacggt agtgttcaat cttgtcaatg
    17941 acttccttaa tggcatgttt attttgtgct gaagttagga ccacatcgac tggacgaaga
    18001 ccttcttcat gggcacgttt catgagccac tggctaatct taccagactt aactgactta
    18061 ggaaggatat cttttttatt tcctaccaag aggacatcat tgcccgagac gaaacgtggt
    18121 aaacctggga tgacagatcc attaaaatca aagatatcaa taacattgac cactaaagca
    18181 tcactgtctc ccacctcgtg caagagcttg aggaaatcat cgttcgtcaa ctggacatct
    18241 gtgatttcat tgtagtggcg gagacggaaa cagcgttggc aatagacttc gccagtctcc
    18301 aaaccttttt caagtgccga ctggggggta aaaccaagac cagctttatc tgtcgtctga
    18361 atggttgctc cacaaccaat acagagaatt tcttccatag ttaaattcct tttttatatg
    18421 taatcggtcc gtacttttca gtgatttttc tcataacacg acgctcacga gttcggttaa
    18481 tctgcgtttt gattgagtca tgttggacca agggtttgac taaaattgac cgaatccctg
    18541 cacggtgggc tgctcgtata tctgtcatga gttggtcacc aaccatgacc acttcctttt
    18601 tgtcatagtg gaattccttc atagcacggt caataccaaa tgtgaagggc ttcaaggccc
    18661 agtaaacgta atcaatccca aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat
    18721 ttgacactac gataatgcca atacccgcgt cccgaaggtc atgtagccat tgcttcatct
    18781 ctggcgttcc atcagggttg ttccaagcaa tgagggtatt atccaaatcg accaaaacag
    18841 cctttattcc ctgcgcctgc aggcttggga ctgtcagatc atagactgct tccacagcaa
    18901 aatctggtat ataattttca atcgccattc tggctccttt tcttaacttt ttttcgcaat
    18961 tttccttagt aacaaggata aaacaatcca caaacctgca ctagccaaac tgatatagag
    19021 ccaagcatgg ggctcatctg ttaaaggtaa gggaacattc attccgaaaa agcctgtcac
    19081 gactgccaaa acagctagca agactgaaat gatagtcaag gttgtcaaat tgtcattcag
    19141 attattgttt agaatattgt tgtaagaggc tgaaagctgc tgtaaaatct gagagattag
    19201 gtctgtcatg gataccagct gatgagcctc aatcatggca tcatcaaact gttctctctc
    19261 aatctcatca aaactacgat acaaggcatg accttgaata tgctctaaca aaatccgatt
    19321 ttgtttggca gctgccgtca gataaaccat accagtctcc aaatcagaca ggacaaagag
    19381 gtttttcttg gtagttcgct ggcgcaagag gtcattgacc tcatccctac tcttgtccat
    19441 ctgctcaatg acaggatagt aggcattgct gatgatttcc agactggcaa agagaaactt
    19501 ataaatcgaa agcgtgtcat ggttctccag ataacgagtc atctgttcaa tgacataggc
    19561 gttcttggta ttactaatgg taatcaggcg acgatgctcg acaataaagg tcatgggaaa
    19621 agtctcatag taggccttgt ccttttttac gtctaagaca ttatagataa aggtaaccgt
    19681 ctcactttca cggtggtagt ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat
    19741 ggtttccttg tccaaaccgt aaatttcaga aaggtcttct agtttattca actttcctag
    19801 gtctaggtct atccaggtac aaccattgcc caactgcttt tctaaaacca tcttttctcc
    19861 ttattaaact ccctctattg taccacaaat tacttaaaat aacaggtcta gtctgtgcgt
    19921 gagaaattgc taacaactgt gatatttcgg ttagggacaa agtgaagggc ttgctcctcg
    19981 ctacgaagct gtgtcgtacg aattcccaca ctgacaacct tacccgatac agtaatcggt
    20041 ccatttgtca gaacgacctc atctcccaca tccagttgac gttcaaagag gatgaaaaag
    20101 ccattgatga catcagacag aaagccttgg gctcccatac caatcgctac cccagcaata
    20161 ccagctccag ccagcaaact agaaactggc aaacctaaaa tcgacaaaat gcagtagagt
    20221 aaaaagaaat atagcgtata attaaacaca ttttctagta aacgtgagat ggttttttgg
    20281 cgtccaacat catgacgaga catttttaga gaaggtttga caattctctg caccatggta
    20341 tgaagcattt ttttagcaat ataaaataca attaaaagca gtaaaagaga aatgaccttg
    20401 gttagaatat tctcgataat ggttgtcaca tctagctttt caatataagc ctgaataaat
    20461 ttttgcatat aaaactcctt tatttcatta taccatattt tcatcagtct cattctccat
    20521 aaatattcaa aaaatagaca gaaaattctt gattttgtct atcatttata ctataatcat
    20581 aatcactaca caaaaaggag attattatga aaagaatcat tcatgcttgg aataaggcaa
    20641 gccttgtcaa gcgtatcttg attggtatgc tttgtgggga atcctaggac tgacccttcc
    20701 taatctctca gggattgggc tactcgagga tttattcgtt ggaggtctaa aagctgttgc
    20761 tcctatccta gtctttgctc tcgttgccaa tgccctttcc caacatcaaa agggacaaga
    20821 tagcaatatg aaaactgttg ttttcttgta tatcctatga tagggacttt cgccgctgct
    20881 cttgtagctg tactagcaaa tttcatcgtc cctattgaaa ttaccccaaa tagtgccaat
    20941 actgaaattg caccaccaga tgggattggg caggttctca gcaacctctt gctcaaactg
    21001 gttgacaacc cagtcaacgc cctgcttact gctaactata ttagaatctt atcttgggca
    21061 gtcatttttg gaatcgctat gagagaagcc agtaaaaata gtcaagaatt gctaaaaact
    21121 atcgctgacg tgacttctaa aattgtcgaa tggatcatca atctggctcc atttggaatc
    21181 cttggtcttg tttttaaaac catttctgac aagggagtcg gaagccttgc caactacggt
    21241 attttattgg ttctattagt aacgactatg ctttttgttg cccctgtggt caaccctttg
    21301 attgccttct tctttatgag acgcaatcct taccctctag tttggaactg cctccgtgtc
    21361 agcggtgtga cagccttttt cactcgtagt tctgcgacta acattcctgt caacatgaaa
    21421 ctctgccatg accttggact caacccagat acctattctg tttctatccc actcggttct
    21481 actatcaata tggctggagt agcgattacc attaaccttt tgacccttgc tgcagttaac
    21541 actcttggaa ttcctgttga ctttgccaca gcctttgtcc tcagtgtggt agcagctatc
    21601 tcatcctgtg atgcttcagg tattgccgga ggttccctcc ttcttatccc agttgcttgt
    21661 agccttttcg gtatttctaa cgatattgcc atacaaattg ttggggttgg ttttgtgatt
    21721 ggtgtcatcc aagactcatg tgaaacagcc cttaactctt ctacagatgt cctctttacc
    21781 gccgttgccg aatacgcagc aacccgtaaa aaataactca tcaaggcaag cctgctcatg
    21841 tcttgtcttt tacgctttta ttctaactta ttaggaaatt cttatgtcta ttagccaacg
    21901 tacgaccaag ctcatcttag ctacctgtct tgcctgcctg cttgcttatt ttctcaatct
    21961 ttcgtcagca gtttcggctg gaattatcgc tctcttgagc ctatctgata cgcgtagaag
    22021 tactttaaaa ctggctcgca atcgtctttt ttctatgctt ctagctctgg ctatcggtgt
    22081 tctagctttt cacttgagcg gatttcatat ctggagtctc ggcctctatc tggccttcta
    22141 cgttccttta gcctacaaga tgggctggga aattggcatc acaccaagca ctgttttggt
    22201 tagccatctc ttggttcaag agtcaacctc tccagacctt ctagtcaatg aattccttct
    22261 ctttgctatt ggtacaggat ttgccttgct tgttaatctc tatatgcctt cacgagaaga
    22321 ggaaatccag cactaccaca cgctggtgga agaaaagtta aaagatatcc tccagcgctt
    22381 caaatactat ttatccagag gagacggacg caaccgagca cagctggtag cagaattaga
    22441 cacgcttttg aaagaagccc tcagactggt ctatttggat cactctgacc acctctttca
    22501 ccagacagac taccatatcc actactttga gatgagacag cgacaaagtc gtatcctgag
    22561 aaacatggcc caacagatta acacttgtca ccttgccgcc agtgaaagcc tgatcttagc
    22621 gcaactcttt tcaaaaattg caggtcaact gagccagacc aatcctgctt ctgatttgct
    22681 agatgaaatt gaacgttatc tggaagtctt ccggaaccgc agtctgccca agacaagaga
    22741 agaatttgaa acccgcgcca cccttcttca actcctacgt gaagccaaaa ccttcatcca
    22801 agtaaaagtt gatttttacc aaaaatatag acagtaatac tcttcaaacc acgtcagcgt
    22861 cgccttgctg tatatatgtt
//
LOCUS       DBDESG010000033        22849 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.033, whole genome shotgun sequence.
ACCESSION   DBDESG010000033 DBDESG010000000
VERSION     DBDESG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22849
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.033"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            278..2065
                     /locus_tag="ACKB38_001260"
     CDS             278..2065
                     /locus_tag="ACKB38_001260"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGL7980026.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPCEGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2062..2538
                     /locus_tag="ACKB38_001261"
     CDS             2062..2538
                     /locus_tag="ACKB38_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7980027.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILGGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2566..3471
                     /locus_tag="ACKB38_001262"
     CDS             2566..3471
                     /locus_tag="ACKB38_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGL7980028.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3458..4273
                     /locus_tag="ACKB38_001263"
     CDS             3458..4273
                     /locus_tag="ACKB38_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGL7980029.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4280..4684
                     /locus_tag="ACKB38_001264"
     CDS             4280..4684
                     /locus_tag="ACKB38_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7980030.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4983..6149
                     /locus_tag="ACKB38_001265"
     CDS             4983..6149
                     /locus_tag="ACKB38_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGL7980031.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6265..7302
                     /locus_tag="ACKB38_001266"
     CDS             6265..7302
                     /locus_tag="ACKB38_001266"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGL7980032.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7464..7724
                     /locus_tag="ACKB38_001267"
     CDS             7464..7724
                     /locus_tag="ACKB38_001267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose-binding lectin II"
                     /protein_id="HGL7980033.1"
                     /translation="MALTLAGAVLTNDVFANDRLVATQTTDGKNENVLTSEVLKPSSG
                     NVLVGIKGEFVAPHQQSILDAINAICKEAADEGLVDKYVPIK"
     gene            7773..8632
                     /locus_tag="ACKB38_001268"
                     /pseudo
     CDS             7773..8632
                     /locus_tag="ACKB38_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            9084..14654
                     /gene="zmpC"
                     /locus_tag="ACKB38_001269"
     CDS             9084..14654
                     /gene="zmpC"
                     /locus_tag="ACKB38_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HGL7980034.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            15010..15864
                     /locus_tag="ACKB38_001270"
     CDS             15010..15864
                     /locus_tag="ACKB38_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGL7980035.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            15970..16527
                     /locus_tag="ACKB38_001271"
     CDS             15970..16527
                     /locus_tag="ACKB38_001271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGL7980036.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            16744..17508
                     /locus_tag="ACKB38_001272"
     CDS             16744..17508
                     /locus_tag="ACKB38_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGL7980037.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            18121..19500
                     /locus_tag="ACKB38_001273"
     CDS             18121..19500
                     /locus_tag="ACKB38_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGL7980038.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            19514..20179
                     /locus_tag="ACKB38_001274"
     CDS             19514..20179
                     /locus_tag="ACKB38_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGL7980039.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            20489..21028
                     /locus_tag="ACKB38_001275"
     CDS             20489..21028
                     /locus_tag="ACKB38_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7980040.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLVEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            21095..21505
                     /locus_tag="ACKB38_001276"
     CDS             21095..21505
                     /locus_tag="ACKB38_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGL7980041.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            21621..>22849
                     /gene="pavB"
                     /locus_tag="ACKB38_001277"
     CDS             21621..>22849
                     /gene="pavB"
                     /locus_tag="ACKB38_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGL7980042.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRA"
BASE COUNT         6981 a         4105 c         5130 g         6633 t
ORIGIN      
        1 ataaaaaatt agaataatag tttgaaaaat taagcgatat agggattgct catattttat
       61 aaatgtattc tatacaaaat tattttaggt cctatattcc gaatttaaag agcgtttgaa
      121 atgggtctaa aaatgtttga aactaattga atgtgtgtat aatagtaaaa atgcagtaaa
      181 tatcccgaca atttctattg acaattcaaa cagattggtt tataattaat ataacaacaa
      241 atgaaagcgc aaactttcgc ggtcggaagg tagttttatg acacgatttg agatacgaga
      301 tgatttctat ctcgatggaa aatcatttaa gattttatct ggtgccattc attattttag
      361 ggttcctcca gaggattggt atcattcgct ctataacttg aaggctcttg gttttaatac
      421 ggtagagact tatgttgctt ggaatttaca cgagccttgt gaaggtgagt ttcattttga
      481 aggtgatctg gatttagaga aatttctcca aatagcgcag gatttgggtc tctacgcaat
      541 tgtgcgtccg tctccattta tctgtgcgga atgggaattc ggtggcttac cagcttggct
      601 cttgaccaag aacatgcgaa ttcgctcatc cgacccagca tatatcgagg cagttggtcg
      661 ctactatgat cagttattgc caagactggt gcctcgtttg ttggacaatg gtggcaatat
      721 tctcatgatg caggttgaaa atgagtatgg ttcttacgga gaagataagg cttacctgag
      781 agcgattcga cagctaatgg aagagtgtgg cgtaacctgt cccctcttta catcagatgg
      841 tccatggcga gctactctga aagctggaac cttaattgaa gaggacctct ttgtaacagg
      901 aaactttggt tctaaggcac cttacaactt ttcgcagatg caggaattct ttgatgaaca
      961 tggtaagaaa tggccactca tgtgtatgga gttctgggat ggttggttca atcgctggaa
     1021 agaaccgatt atcacacggg atcctaagga attggcagat gcagttcgag aggttttgga
     1081 acaaggctct atcaatcttt acatgttcca cggtggtaca aactttggtt tcatgaatgg
     1141 ttgctcagct cgaggaactt tggacctgcc acaagttacg tcttatgatt acgatgccct
     1201 tctggatgaa gaaggaaatc caactgctaa atatcttgca gtcaagaaga tgatggcaac
     1261 acatttttca gagtatccgc agttggaacc actctacaaa gagagtatgg agttggatgc
     1321 tattccacta gttgaaaaag tttctttgtt tgaaacctta gatagcttgt caagtcctgt
     1381 agaaagtctc tatcctcaaa agatggagga gctgggacaa agttatggct acctacttta
     1441 tcgaacagaa acaaactggg atgcagaaga agaaagactt cgtatcattg atggtcgaga
     1501 tagggcccag ctgtatgtcg atggtcagtg ggttaaaact caatatcaga cagagattgg
     1561 ggaagatatt ttttatcaag gtaaaaagaa agggctatct aggttagata tcttgataga
     1621 aaatatgggg cgtgtcaact atgggcataa gttcttagcg gatacgcaac gtaagggaat
     1681 tcggacaggg gtctgtaagg atctgcattt cttactaaac tggaaacact atccactccc
     1741 actagacaat cctgagaaaa ttgatttttc aaaaggatgg actcaaggac aaccagcctt
     1801 ttacgcttat gactttacag tcgaagagcc aaaagatact tacctagact tgtctgagtt
     1861 tggtaagggg gttgcctttg tcaatgggca gaatctagga cgtttttgga acgttggccc
     1921 aactctctca ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc gcatcattat
     1981 ctttgaaaca gaaggtcaat ataaagaaga gattcattta actcgtaaac ctacactaaa
     2041 acatataaag ggggaaaact tatgacaatt gtaggatgcc gtattgatgg acgtttgatc
     2101 cacggacaag tagccaatct ttgggctgga aaactaaatg tttcacgcat tatggttgta
     2161 gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc gacaccacca
     2221 ggtgtgaaat tgagtatttt gccagttgag aaagctgcag ccaatattct tggtggcaaa
     2281 tacgatagcc aacgtctctt tatcgtggct cgtaaaccag accgcttcct tggtttggta
     2341 gaagcaggtg taccacttga aacccttaat gttgggaata tgtctcaaac accagaaact
     2401 cgttctatta cacgttctat caacgtagta gacaaggatg tggaagactt ccacaaactg
     2461 gcagaaaaag gtgttaaact tactgctcag atggttccaa atgatccaat ttcagacttt
     2521 ttgagcttat taaaatagga aaaaaatttt taggaggtca ttgttatgat acaatggtgg
     2581 caaattttac ttctcacttt gtactcagct tatcaaatct gtgatgagtt gacgatcgtt
     2641 tcatctgcag gttcccctgt atttgctggt ttcattactg gtttaatcat gggagatgtg
     2701 actactggtt tacttatcgg tggtaacttg caactgttcg ttcttggggt tggtaccttc
     2761 ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc cttctctgtt
     2821 tcacaaggaa ttgatgcacc gcttgccatt actacaatcg ctgtaccagt agcagctctc
     2881 ttgacttact tcgacgttct tggtcgtatg actactacct tcttcgctca ccgtgtggat
     2941 gctgcaatcg aacgctttga ctataaaggt attgaacgca actacttgct tggtgcgatt
     3001 ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg tggtgccttt
     3061 gtacaatcag tagtagactt cgttgaagcc tacaaatggg ttgcagatgg cttgacactt
     3121 gcaggacgta tgcttccagg tcttggattt gcaatcttgc ttcgttacct tccagttaaa
     3181 cgtaaccttc actaccttgc tatgggattt ggtttgacag ctatgttgac tgttctttac
     3241 tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg taggtactct tcctgctgaa
     3301 gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat tggtatttct
     3361 atcgtaggta ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt agctgtagca
     3421 gcaccttcta caccatcaga aagtggggaa atcgaagatg acgaattcta attacaaact
     3481 tacaaaagaa gattttaatc aaatcaacaa acgtagcttg tttactttcc aattaggttg
     3541 gaactacgaa cgtatgcaag cttctggtta cctttacatg atcttgcctc agttgcgtaa
     3601 aatgtatggt gatggaactc ctgaattgaa agaaatgatg aaagttcata ctcaattctt
     3661 caatacttca ccattcttcc ataccattat cgctggtttt gaccttgcca tggaagaaaa
     3721 agatggtgta ggttcaaaag acgccgttaa cggtatcaag acaggtttga tgggaccatt
     3781 cgctcctctt ggggatacaa tctttggttc acttgtacct gctatcatgg ggtcagtcgc
     3841 agcaactatg gctatcgctg gccaaccttg ggggatcttc ctttggattg cagttgcagt
     3901 agcgtatgac atcttccgtt ggaaacagtt ggaatttgct tacaaagaag gggttaacct
     3961 tatcaacaac atgcaaagta ccttgacagc tttgattgac gctgcatctg tacttggtgt
     4021 cttcatgatg ggtgctcttg tagcaacagt gattaacttt gaaatttctt acaagttgcc
     4081 aatcggtgaa aagatgattg atttccaaga catcttgaac caaatcttcc cacgtttgct
     4141 tccagcaatc tttactgcct ttatcttctg gttgcttggt aagaaaggta tgaactctac
     4201 taaagctatc ggtattatta tcgtacttgc tttggctctt tctgcccttg gtcactttgc
     4261 acttggaatg taattcctta tgactaaatc attaattttg gtgagccatg gtcgcttctg
     4321 tgaggagctt agaggtagca cagaaatgat tatgggccca caagacaaca tttacacagt
     4381 agctcttctt ccagaagatg gcccagaaga atttactgct aaatttgaag ctgttattga
     4441 aggattggat gatttcctag tctttgcgga tcttctcggt gggacacctt gtaatgtggt
     4501 gagtcgcttg atcatggaag gtcgtgatat tgacctttac gcagggatga atcttccaat
     4561 ggtgattgaa tttatcaatg cgagccttac aggcgcagat gcggactaca agagccgtgc
     4621 tgcagaaagc attgtgaaag ttaatgacct gttagcgggc ttcgatgatg acgaagatga
     4681 ataatactct tcgaaaatct cttcaaacta cgtcaacgtc gccttgccgt aggtatatgt
     4741 tactgacttc gtcagtctta tccggcaacc tcaaaacggt gttttgagct gacttcgtca
     4801 gtcttatccg gcaacctcaa agcagtgctt tgagcagcct gcggctagtt tcctacagat
     4861 tttagttgga actcgattca attcatgtga caacgtgaaa atcgttagag cattttatat
     4921 agaatataca tgggaatgta gcttactccc attcccatat ttaatagaaa aagaggaact
     4981 caatgctaca ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc actacgcgtg
     5041 aaatctacca acagcctgat gtatggagag aagcttttga attttatcaa gcaaaacgtg
     5101 aagaaattgc agccttccta caagaaatcg ctgataaaca tgactatatt aaggttatct
     5161 tgacaggtgc tgggacttct gcttatgtgg gagatacctt gctaccttat tttaaggaag
     5221 tctatgacga acgcaaatgg aatttcaatg ctattgcgac aacagatatc gttgccaatc
     5281 cagcaaccta tttgaaaaaa gatgtggcaa ctgtccttgt gtcttttgct cgtagtggga
     5341 attcgcctga aagtttggcg actgttgatt tggccaaatc cttggtggat gagctttatc
     5401 aagtgacgat tacttgtgca gcagatggta aattggctct tcaagctcac ggtgatgatc
     5461 gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc atgacttcta
     5521 gctttacgtc tatgatgttg acaactctct tggtctttga tcctacagaa tttgctgtta
     5581 agtctgaacg ttttgaagtt gtatctagtc ttgcccgtaa agttttagac aaggcagaag
     5641 atgtcaaaga gctcgttgat ttagacttta accgtgtcat ctatctaggc gctggtcctt
     5701 tctttggact tgctcatgaa gctcagctca agattttgga attaactgct ggtcaagttg
     5761 cgaccatgta tgaaagccca gttggcttcc gtcacggtcc aaaatctctt atcaacgaca
     5821 atacagttgt tttggtcttt ggtacaacga cagactacac tcgtaagtac gacttggact
     5881 tggttcgtga agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg agtgatcaag
     5941 cttttggtct tgaaaatgtc aaagaagtgg cccttggttg tggcggtgtc ttgaatgata
     6001 tttaccgtgt cttcccttac atcgtttatg cccaactctt tgctttattg acttcactca
     6061 aggtagaaaa taaaccagat acaccgtctc ctacaggtac agtaaaccgt gtagtacaag
     6121 gtgtcataat tcacgaatat caaaagtaag acagtgttta tgaattcttg acaagaggat
     6181 ttgtaaatta tcagataaac catagattgt cagtacgctt tctatggttt gtttgcttga
     6241 gagaaatagt aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt tggaaatgtt
     6301 gagggtgagg atgtcttggc ctatcgattt gagacagacg gtggctacca acttgaggtt
     6361 atgacttatg gtgcgactat cttgcgctat gtcgcacctg acaaggctgg aaattttgcc
     6421 aatgttatct tgggatttga tgactttgat agttatgtag gcaatagtcc caagcatgga
     6481 gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct caatggtaag
     6541 acctatgacc ttgaggttaa taatgctagc aactgtaatc acagtggttc aactggttgg
     6601 gattccagct tgtttgaagt tgaagaagta agcgatcatg gcttgactct ctacacagag
     6661 cgtacagatg ggacaggagg gttccctgga aatctcaaga tttggatcag ttatcacttg
     6721 gaagaaactg gtgcctatga aatcagctac aaggtaacga ccgatcagga tacgctggtc
     6781 aatccaacca accacagcta tttcaacttg tctggtgatt tcacgcagac gattgaccgt
     6841 catgtcttcc aactaaacac agagggcatt tactcaatcg ctcctgacgg tgttcctgcc
     6901 aaaactccag aagccaaccg tgatgtggtc aaacacgtct acaatggtac cttgttgaag
     6961 gatatctttg cagaagaaga tgagcaaatc cagctggcat caggtttgga tcatccattt
     7021 gcccttcctg caggccatga caatgctgga ttcctttatg accaaaattc aggtcgcttc
     7081 ctgcttttca agacagaagc tccttgcttt gtggtctaca cagcaaactt tgtggatgaa
     7141 agtgtcatca taggaggtca gccaatgcta cagcacaatg ggattgctct tgaagcgcaa
     7201 gctttaccag atgccattca cagtgacctt aaaggccaag tcattcttaa agctggtcaa
     7261 accttcacca gtaagacacg ttatgaactt gttgtgaagt aaaagagtca ttgcgcctac
     7321 ttttgggagc taggaatagg tacgcagaga caaatagtag gaaaatatga tataactaag
     7381 cgttgaaagc tatctgttaa tataatattc aaactacaat aaggagtaag aaagaaacga
     7441 agaaaattgt atttgctagt gccttggctt tgaccttggc tggagcagtt ttgacaaatg
     7501 atgtttttgc gaacgacaga cttgtggcaa cacaaactac tgatggtaaa aatgaaaatg
     7561 tattgacctc agaggtgcta aaaccttcta gtggcaatgt tttggttgga atcaaaggag
     7621 aatttgtggc tcctcatcaa caatctattt tggatgccat caatgctatc tgtaaagaag
     7681 cggctgacga aggtttggta gataagtatg tccctatcaa atgatcaact gacctagaaa
     7741 aggcagcttt tgccagagct acagaagcat ctataaccat ggatcatacc cgtctttcta
     7801 gcaaagatct ttggagtgcc tttccaactt ctaatagtat aatgggagaa aatttggcat
     7861 ggaatcatga cggttttcta aaagctattg aacaatggcg tgctgaaaaa gcagattatg
     7921 tggagaaaaa aatagtggtt cagacaacgg gaaatctggt cactatgagt cgctaattaa
     7981 ccctaaattt acacacatgg ggatggcagc ttttaaaaat cctaacaatc aatacaaagc
     8041 tattacaatt gctcaaactc taggtgatga tgcttcttca gaggaattgg ctggtagata
     8101 tggttctgct gttcagtgta cagaagtgac tgcctcaaac ctttcaacag ttaaaactaa
     8161 agctacggtt gtagaaaaac cactgaaaga ttttagagcg tctacgtctg atcagtctgg
     8221 ttgggtggaa tctaatggta aatggtattt ctatgagtct ggtgatgtga agacaggttg
     8281 ggtgaaaaca gatggtaaat ggtactattt gaatgactta ggtgtcatgc agactggatt
     8341 tgtaaaattt tctggtagct ggtattactt gagcaattca ggtgctatgt ttacaggctg
     8401 gggaacagat ggtagcagat ggttctactt tgacggctca ggagctatga agacaggctg
     8461 gtacaaggaa aatggcactt ggtattacct tgacgaagca ggtatcatga agacaggttg
     8521 gtttaaagtc ggaccacact ggtactatgc ctacggttca ggagctttgg ctgtgagcac
     8581 aacaacacca gatggttacc gtgtaaatgg taatggtgaa tgggtaaact aggctcaggc
     8641 cataggtaaa gcattcatct tacttagcaa aaagaatgaa cgataagaaa gaggttgatg
     8701 gcgaacattg gcctcttttg atttataaag attggattct tgtcgcctca atttcagact
     8761 tttctattgt aagctaatat tttatagccc attaaaagca taagcggtaa tctaatttaa
     8821 aaaatgctgt aattagtctg aagtccacac ttacttgttg agatgttatc tctgtttttt
     8881 atcgttataa tttactgtat tttttatagt atgcagaata tttttaagta tatttcaata
     8941 gaaatttcta tcgatttatt gtataatgat aagtaattgt tgaaaagtac tcagaaaatt
     9001 ccatactata ttatttttat gtttatactt ttatgctata aaatatagat tgatataaag
     9061 aatatagaaa aagcgaggtt aatatgagcc gaaaaagcat tggtgagaaa cgccatagtt
     9121 tctcgatgag aaagttgtca gtgggattgg tatcagttac tgtatctagt ttctttttga
     9181 tgagtcaagg gattcaatcg gtatcggccg ataatatgga aagtccaatt cattataagt
     9241 atatgaccga gggtaaattg acagacgagg aaaaatcctt gctggtagag gcccttccac
     9301 aactggctga agaatcagat gatacttatt acttggttta tagatctcaa cagtttttac
     9361 cgaatacagg ttttaaccca actgttggta ctttcctttt tactgcagga ttgagcttgt
     9421 tagttttatt ggtttctaaa agggaaaatg gaaagaaacg acttgttcat tttctgctgt
     9481 tgactagcat gggagttcaa ttgttgccgg ccagtgcttt tgggttgacc agccagattt
     9541 tatctgccta taatagtcag ctttctatcg gagtcgggga acatttacca gagcctctga
     9601 aaatcgaagg ttatcaatat attggttata tcaaaactaa gaaacaggat aatacagagc
     9661 tttcaaggac agttgatggg aaatactctg ctcaaagaga tagtcaacca aactctacaa
     9721 aaacatcaga tgtagttcat tcagctgatt tagaatggaa ccaaggacag gggaaggtta
     9781 gtttacaagg tgaagcatca ggggatgatg gactttcaga aaaatcttct atagcagcag
     9841 acaatctatc ttctaatgat tcattcgcaa gtcaagttga gcagaatccg gatcacaaag
     9901 gagaatctgt agttcgacca acagtgccag aacaaggaaa tcctgtgtct gctacaacgg
     9961 tgcagagtgc ggaagaggaa gtattggcga cgacaaatga tcgaccagag tataaacttc
    10021 cattggaaac caaaggcacg caagaacccg gtcatgaggg tgaagccgca gtccgtgaag
    10081 acttaccagt ctacactaag ccactagaaa ccaaaggtac acaaggaccc ggacatgaag
    10141 gtgaagctgc agttcgcgag gaagaaccag cttacacaga accgttagca acgaaaggca
    10201 cgcaagagcc aggtcatgag ggcaaagcta cagtccgcga agagactcta gagtacacgg
    10261 aaccggtagc gacaaaaggc acacaagaac ccgaacatga gggcgaagcg gcagtagaag
    10321 aagaacttcc ggctttagag gtcactacac gaaatagaac ggaaatccag aatattcctt
    10381 atacaacaga agaaattcag gatccaacac ttctgaaaaa tcgtcgtaag attgaacgac
    10441 aagggcaagc agggacacgt acaattcaat atgaagacta catcgtaaat ggtaatgtcg
    10501 tagaaactaa agaagtgtca cgaactgaag tagctccggt caacgaagtc gttaaagtag
    10561 gaacacttgt gaaagttaaa cctacagtag aaattacaaa cttaacaaaa gttgagaaca
    10621 aaaaatctat aactgtaagt tataacttaa tagacactac ctcagcatat gtttctgcaa
    10681 aaacgcaagt tttccatgga gacaagctag ttaaagaggt ggatatagaa aatcctgcca
    10741 aagagcaagt aatatcaggt ttagattact acacaccgta tacagttaaa acacacctaa
    10801 cttataattt gggtgaaaat aatgaggaaa atactgaaac atcaactcaa gatttccaat
    10861 tagagtataa gaaaatagag attaaagata ttgattcagt agaattatac ggtaaagaaa
    10921 atgatcgtta tcgtagatat ttaagtctaa gtgaagcgcc gactgatacg gctaaatact
    10981 ttgtaaaagt gaaatcagat cgcttcaaag aaatgtacct acctgtaaaa tctattacag
    11041 aaaatacgga tggaacgtat aaagtgacgg tagccgttga tcaacttgtc gaagaaggta
    11101 cagacggtta caaagatgat tacacattta ctgtagctaa atctaaagca gagcaaccag
    11161 gagtttacac atcctttaaa cagctggtaa cagccatgca aagcaatctg tctggtgtct
    11221 atacattggc ttcagatatg accgcagatg aggtgagctt aggcgataag cagacaagtt
    11281 atctcacagg tgcatttaca gggagcttga tcggttctga tggaacaaaa tcgtatgcca
    11341 tttatgattt gaagaaacca ttatttgata cattaaatgg tgctacagtt agagatttgg
    11401 atattaaaac tgtttctgct gatagtaaag aaaatgtcgc agcgctggcg aaggcagcga
    11461 atagcgcgaa tattaataat gttgcagtag aaggaaaaat ctcaggtgcg aaatctgttg
    11521 cgggattagt agcgagcgca acaaatacag tgatagaaaa cagctcgttt acagggaaac
    11581 ttatcgcaaa tcaccaggac agtaataaaa atgatactgg aggaatagta ggtaatataa
    11641 caggaaatag ttcgagagtt aataaagtta gggtagatgc cttaatctct actaatgcac
    11701 gcaataataa ccaaacagct ggagggatag taggtagatt agaaaatggt gcattgatat
    11761 ctaattcggt tgctactgga gaaatacgaa atggtcaagg atattctaga gtcggaggaa
    11821 tagtaggatc tacgtggcaa aacggtcgag taaataatgt tgtgagtaac gtagatgttg
    11881 gagatggtta tgttatcacc ggtgatcaat acgcagcagc agatgtgaaa aatgcaagta
    11941 catcagttga taatagaaaa gcagacagat tcgctacaaa attatcaaaa gaccaaatag
    12001 acgcgaaagt tgctgattat ggaatcacag taactcttga tgatactggg caagatttaa
    12061 aacgtaatct aagagaagtt gattatacaa gactaaataa agcagaagct gaaagaaaag
    12121 tagcttatag caacatagaa aaactgatgc cattctacaa taaagaccta gtagttcact
    12181 atggtaacaa agtagcgaca acagataaac tttacactac agaattgtta gatgttgtgc
    12241 cgatgaaaga tgatgaagta gtaacggata ttaataataa gaaaaattca ataaataaag
    12301 ttatgttaca tttcaaagat aatacagtag aatacctaga tgtaacattc aaagaaaact
    12361 tcataaacag tcaagtaatc gaatacaatg ttacaggaaa agaatatata ttcacaccag
    12421 aagcatttgt ttcagactat acagcgataa cgaataacgt actaagcgac ttgcaaaatg
    12481 taacacttaa ctcagaagct actaaaaaag tactaggagc agcgaatgat gcagccttag
    12541 ataacctata cttagataga caatttgaag aagttaaagc taatatagca gaacacctaa
    12601 gaaaagtatt agcgatggat aaatcaatca atactacagg agacggtgta gttgaatacg
    12661 taagtgagaa aatcaaaaat aacaaagaag catttatgct aggtcttact tatatgaacc
    12721 gttggtacga tattaattat ggtaaaatga atacaaaaga tttatctacg tacaagtttg
    12781 actttaacgg aaataatgag acttcaacgt tggatactat tgtcgcatta ggaaatagtg
    12841 gactagataa cctgagagct tcaaatactg taggtttata tgcgaataaa cttgcatcgg
    12901 taaaaggaga agattcagtc tttgacttcg tagaagcgta tagaaaactg ttcttaccaa
    12961 acaaaacaaa taacgagtgg tttaaagaaa atacaaaggc atatatagtc gaaatgaagt
    13021 ctgatattgc agaagtacga gaaaaacaag aatcaccaac agccgataga aaatattcat
    13081 taggagttta cgatagaata tcagcaccaa gttgggggca taagagtatg ttattaccac
    13141 tactaacttt acctgaagaa tctgtgtata tttcatcgaa tatgtctaca cttgcattcg
    13201 gttcgtatga aagatatcgt gatagtgtgg atggagttat tctttcagga gatgctttac
    13261 gaacttatgt aagaaataga gttgatatag cagcgaaaag gcatagagac cattatgata
    13321 tttggtacaa tcttcttgac agtgcttcaa aagaaaaact tttccgttct gtgatagttt
    13381 atgatggatt caatgtaaaa gatgagacag gaagaactta ttgggcaagg ttaacggata
    13441 aaaacatcgg ctctattaaa gaattcttcg gacctgttgg gaaatggtat gagtataata
    13501 gtagtgcagg agcgtatgcg aatggaagtt taacgcactt tgtgttagat agattattag
    13561 atgcttatgg aacgtcggtt tatactcatg aaatggttca taattctgat tctgcaatct
    13621 actttgaagg aaatggtaga cgtgaaggat tgggagcgga gttatacgca cttggtttac
    13681 tgcaatctgt agatagtgta aattctcata ttttagcttt aaatacgtta tataaagcag
    13741 aaaaagatga tttgaataga ttgcatacat ataatccggt ggaacgtttc gattcggatg
    13801 aggcgcttca aagttatatg catggatcat atgatgtaat gtatacactt gatgcgatgg
    13861 aagcaaaagc gatattagct caaaataatg atgttaagaa aaaatggttt agaaaaatag
    13921 aaaattatta cgttcgtgat actagacata ataaagatac acatgcagga aataaagtcc
    13981 gtccattaac agatgaagaa gtagctaact taacatcgtt aaactcatta atcgacaacg
    14041 acatcataaa tagacgtagc tatgatgata atagagaata taaacgaaat ggctactata
    14101 ctataagtat gttctctcct gtatacgcag cgctaagcaa ttcgaaaggt gctcctggag
    14161 atattatgtt tagaaaaata gcttatgaat tacttgcgga aaaaggttat cacaaaggat
    14221 tcctacctta tgtttctaat cagtacggag cagaagcatt tgccagcgga agcaaaacat
    14281 tctcatcatg gcatggaaga gatgttgctt tagtgacaga tgatttagta tttaagaaag
    14341 tattcaatgg tgagtactca tcatgggctg atttcaaaaa agcaatgttt aaacaacgta
    14401 tagataaaca agataatctg aaaccaataa caattcaata cgaattaggt aatcctaata
    14461 gtacaaaaga agtaactata acaacggctg cacaaatgca acaattaatt aatgaagcgg
    14521 ctgcgaaaga tattactaat atagatcgtg caacgagtca taccccagca agttgggtgc
    14581 atttattaaa acaaaaaatc tataatgcat atcttcgcac tacagatgac tttagaaatt
    14641 ctatatataa ataagattgt agagtttcat tgttgagtag tgtttcttgt aaggatgagg
    14701 agtcagatga caaatcgact cctttttctt atggatcgat gtagagattt gattgaatgc
    14761 agattgcagg aatcatcttc aactcatcaa cgaccaatgg tgacaaggtg gatttcaatc
    14821 ccacagaaaa tgttgatttg agaaataact ttgctagtct agtaaaataa atacaaaaca
    14881 atcctagaag attttttctg ggattgtttt ttgctgagtg ggatgcttca agttgtctgg
    14941 cttgactttc ttgagggaag ttatataata gttgtaataa ttaaagttac aataaaagag
    15001 gaaatattta tgacatatga ataccagagc cacatttatt tagcagaggc agttttaaat
    15061 gtgaaggatt tggtcagtca aacagttttt tatcagcaga ttattggttt agaaatccta
    15121 tctcaaacgg atacagaggt cgttctggga cttggaggaa aagccttggt acacttgatt
    15181 caagcacaag agggtggaga agtaagggaa cattatggtc tttaccatct ggctattctt
    15241 ttgccgacac gaaaggcttt ggcggatgtc ttgaagcacc tgacggattt acagattcct
    15301 cttgttggcg gtgcagatca cggttacagt gaggcccttt acttagagga cttggaggga
    15361 aatggcattg aactctatcg agataagcca gtttccacat gggatattcg agaagatgga
    15421 cgtattatcg gggtgactga agtccttgcg gctcaggata tctatgagtt gggggaaaga
    15481 gtagagcctt ttatcctagc agagggtacg agaatggggc atattcatct ttctgtcaag
    15541 gatagtcgaa agtccagaca gttttatcaa acggtgttag ggctcgagga taaattcagt
    15601 gtgcctagtg ctagttggat cgcagctggg gactaccatc atcatttagc agtcaacgaa
    15661 tggggaggaa aaggtctgga tccgcgtaaa caagtcctac caggtttagc ctactatgtc
    15721 atcgaagtcg cacataaaga agaactgtta acgattgccc aacgagcaca agaagttgac
    15781 gcaccaatca aatggatgac atcgatccaa ttggaaatca cagactcaga tggcatcgtg
    15841 acccgtattc gtttagctag atagatggta tgtgatgaag gtagagcatc aattgtagaa
    15901 agtgctcctt attctagtca gatacgtgga aaatttgata tgatagtagg gaataaggag
    15961 aagaataata tgactagcga ataccagaaa atgatagcag gagagtttta ccgtccatca
    16021 gacccagaac tgagagcctt agctcaggct tctcgccaaa aacaggccgc ctttaacaag
    16081 gaagagaacc ccttgaaggg agccgaaatc atcaagactt ggtttgcctc aaccgggaaa
    16141 aatctttaca tcaacactcg cttgatggtg gactacggtg tcaacatcca tctaggggaa
    16201 aatttttatt ctaattggaa cttgaccatg ctggatatct gtcccattcg tatcggggac
    16261 aatgctatga ttggtcctaa ttgtcagttt ttgacacccc tccatccact agatccacag
    16321 gaacgcaatt caggtatcga gtacggaaag cctatcacaa tcggagataa tttctggact
    16381 ggtggtggcg tcattgtcct tcctggagtg acactgggaa ataatgtcgt tgcaggagca
    16441 ggggcagtaa ttaccaaatc ttttggcgac aacgttgtcc tagctggcaa tcctgcgcgc
    16501 gtgattaagg aaatacctgt taaatagaag taaaaaggaa cagctggggt tgtttctttt
    16561 ttgtaggttt catcattttt tacccagttc acatttacct actctatctc ttagcaagtc
    16621 tgtttcatta agcaagttca aagcatctcg taagtgggat gtttttctcc tcagttcatc
    16681 agcttcctcc ttgacactcg gtcagatttt gatacaatag tacaaaatta gaggaggcag
    16741 gctatgattc agaaacatgc gattcctatt ttagagtttg atgacaatcc tcaggcggtt
    16801 atcatgccca atcacgaggg gctggacttg cagttgccaa agaagtgtgt ttatgcattt
    16861 ttaggtgagg agattgaccg ctatgcgagg gaagtagggg cgaactgtgt tggcgaattt
    16921 gtttctgcca ccaagaccta tccagtttat gtcgtgaact acaaggacga ggaggtctgt
    16981 ctggctcagg ctcctgttgg ctccgctcca gcagcccagt ttatggattg gttgattggc
    17041 tatggtgtgg agcagattat ctctactggg acctgtggtg tcctagctga tatagaggaa
    17101 aatgcctttc tagtccctgt tcgcgctctg cgagatgaag gagccagtta ccactatgtg
    17161 gcaccttgtc gttatatgga aatgcagcca gaggctattg ctgctattga ggaagttttg
    17221 gaagacagag ggattcctta tgaagaagtc atgacctgga cgacagacgg tttttaccga
    17281 gaaacggctg aaaaggtggc ttatcgtaag gaagaaggct gtgctgttgt ggagatggag
    17341 tgttctgctc ttgcggcagt agctcaattg cgtggggttc tctggggtga attgttgttc
    17401 acagcagatt ctctagcgga cttggaccag tacgacagtc gtgactgggg ctcggaagct
    17461 tttaataagg cgctagaact gagtttagca agtgttcacc acctttagtt gtactggcaa
    17521 aggatttgtt ttatcataaa atgtctagct catacttttc aaaaatatgt ttaaacgagg
    17581 tcaccttcct cttgtcctag gcatgttgag gttgggaaaa atctttaaaa tcagaaaaac
    17641 gtatcatatc aggtgatgaa aactttgaca ctatgcgttt tatgtcgata agatttagag
    17701 tgagatgaaa tgatactctt cgaaaatctc ttcaaaccag gtcagcttca ccttgccgta
    17761 ggtatatgtt actgacttcg tcagtcttat ccggcaacct caaaacggtg ttttgagctg
    17821 acttcgtcag ttctatttgc aacctcaaaa cagtgttttg agcaacctgt gactagcttt
    17881 ctaatcgatg ccttggtttt cattgcctat aatcaaaaag agaaattttc tcctgaaaag
    17941 catatagagt agctggcgtt aaaagctcct gtcttgcttt tttgacctat agtcacatct
    18001 atcaagtatt gttcttgcct aagctatcaa taaaaaggtg gcatttttta ggcttggtgt
    18061 tagtagattt tgccttatcc tatctaagtc atttcgaact ttttatggta caatggaaac
    18121 atgttattca aattatctaa ggaaaaaata gagctaggct tatctcgttt atcgccagcc
    18181 cgtcgtattt ttttgagttt tgccttggtc attttactag gctctcttct tttgagcttg
    18241 ccctttgtcc aagttgaaag ctcacgagcg acttattttg atcatctttt cactgctgtc
    18301 tctgcagtct gtgtgacggg tctctcaacc cttccagtag ctcacaccta taatatctgg
    18361 ggtcaaataa tctgtttgct cttgattcag atcggtggtc tagggctcat gacctttatt
    18421 ggggttttct atatccagag caagcaaaag cttagtcttc gtagccgtgc aactattcag
    18481 gatagtttta gttatggaga aactcgatct ttgagaaagt ttgtctattc tatttttctc
    18541 acgacctttt tggttgagag cttgggagct attttgctta gttttcgcct tattcctcaa
    18601 cttggctggg gacgtggtct ttttagttcc atttttctag cgatctcagc cttctgtaat
    18661 gccggttttg ataatttagg gagcaccagt ttatttgctt ttcagaccga tttactggtc
    18721 aatctggtga ttgcaggctt gattattaca ggcggccttg gttttatggt ctggtttgat
    18781 ttggctggtc atgtaggaag aaagaaaaaa ggacgtctgc actttcatac gaagcttgta
    18841 ctattattga ctataggttt gttgttattt ggaacagcaa ctactctctt tcttgagtgg
    18901 aacaatgctg gaacgattgg caatctccct gttgccgata aggttttagt tagctttttt
    18961 caaacagtga cgatgcgaac agctggcttt tctacgatag attatactca ggctcatcct
    19021 gtgactcttt tgatttatat cttacagatg tttctaggtg gggcacctgg aggaacagct
    19081 gggggactca agattacgac attttttgtc ctcttggtct ttgcacgaag tgagcttcta
    19141 ggcttgcctc atgccaatgt tgcgagacga acgatcgcgc cgcgaacggt tcaaaaatcc
    19201 tttagtgtct ttattatctt tttgatgagc ttcttgatag gattgattct gctagggata
    19261 acagccaaag gcaatcctcc ctttatccac ctcgtatttg aaaccatttc agctcttagt
    19321 acagttggtg taacggcaaa tctgactcct gaccttggga aattggctct cagtgttatc
    19381 atgccactta tgtttatggg acgaattggt cccttgacct tgtttgttag cttggcagat
    19441 taccatccag aaaagaaaga tatgattcac tatatgaaag cagatattag tattggttaa
    19501 gaaaggaaag agcatgtcag atcgtacgat tggaattttg ggcttgggaa tttttgggag
    19561 cagtgtccta gctgccctag ccaagcagga tatgaatatt atcgctattg atgaccacgc
    19621 agagcgcatc aatcagtttg agccagtttt ggcgcgtgga gtgattggtg acatcacaga
    19681 tgaagaatta ttgagatcag cagggattga tacctgcgat accgttgtag tcgcgacagg
    19741 tgaaaatctg gagtcgagtg tgcttgcggt tatgcactgt aagagtttgg gggtaccgac
    19801 tgttattgct aaggtcaaaa gtcagaccgc taagaaagtg ctagaaaaga ttggagctga
    19861 ctcggttatc tcgccagagt atgaaatggg gcagtctcta gcacagacca ttcttttcca
    19921 taatagtgtt gatgtctttc agttggataa aaatgtgtct atcgtggaga tgaaaattcc
    19981 tcagtcttgg gcaggtcaaa gtctgagtaa attagacctc cgtggcaaat acaatctgaa
    20041 tattttgggt ttccgagagc aggaaaattc cccattggat gttgaatttg gaccagatga
    20101 cctcttgaaa gcagatacct atattttggc agtcatcaac aaccagtatt tggataccct
    20161 agtagcattg aattcgtaaa gagggatgac ccctcttttt tgatgcctaa gatggcaaat
    20221 agagacagaa gccccttgtc ttctagtaaa agttcttcaa aggctggact ttatggtaaa
    20281 atagaaagaa gtgacaagag agagtaatac tcaatgaaaa tcaaagatca aactaggaaa
    20341 ctagctacgg gctgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
    20401 aacatctata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtataaga
    20461 aaaaatcagt cccctaaagg agtagattat gaagttattg tctatcgcaa tttctagcta
    20521 taatgcagca gcctatcttc attactgtgt ggagtcgcta gtgattggtg gtgagcaagt
    20581 tgggattttg attatcaatg acgggtctca ggatcagact caggaaatcg ctgagtgttt
    20641 agctagcaag tatcctaata tcgttagagc catctatcag gaaaataaat gccatggcgg
    20701 tgcggtcaat cgtggcttgg tagaggcttc tgggcgctat tttaaagtag ttgacagtga
    20761 tgactgggtg gatcctcgtg cctacttgaa aattcttgaa accttgcagg aacttgagag
    20821 caaaggtcaa gaggtggatg tctttgtgac caattttgtc tatgaaaagg aagggcagtc
    20881 tcgtaagaag agtatgagtt acgattcagt cttgcctgtt cggcagattt ttggctggga
    20941 ccaggtcgga aatttctcca aaggccagta taccatgatg cactcgctga tttatcggac
    21001 agatttgttg cgtgctagcc agttctaact gcctgaacat actttttatg tcgataatct
    21061 ctttgtcttt acgccccttc agcaggtcaa gaccatgtac tatctgcctg tcgatttcta
    21121 tcgttatttg attgggcgtg aggaccagtc tgtcaatgag caagtgatga ttaagtgcat
    21181 tgaccagcaa ctcaaggtca atcgactctt gatagaccaa cttgatttgt cccaagtgag
    21241 tcatcccaaa atgcgagaat atctgctgaa tcatattgaa ctcacgacgg tgatttccag
    21301 taccctgctc aaccgatctg gaacagcgga gcatctggca aaaaaacgcc aattgtggac
    21361 ctatattcag cagaaaaatc cagaagtctt tcaggctatt cgtaagacca tgttgagccg
    21421 tttgaccaaa cattctgtct tgccagatcg caaactgtcc aatgtcgtct atcaaatcac
    21481 caaatctgtt tatggattta attaatataa gtgttttata agagggattt aagaaaaatt
    21541 ttaacttttt cttagtcctt tttaatttca ggagattata ctagagtcat caaataaaga
    21601 aagactctaa ggagaatcct atgaaattca atccaaatca aagatatact cgttggtcta
    21661 ttcgccgtct cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc tttgtcctag
    21721 ttggtcagcc aagttctgta cgtgccgatg ggctcaatcc aaccccaggt caagtcttac
    21781 ctgaagagac atcgggaacg aaagagggtg acttatcaga aaaaccagga gacaccgttc
    21841 tcactcaagc gaaacctgag ggcgttactg gaaatacgaa ttcacttccg acacctacag
    21901 aaagaactga agtgagcgag gaaacaagcc cttctagtct ggatacactt tttgaaaaag
    21961 atgaagaagc tcaaaaaaat ccagagctaa cagatgtctt aaaagaaact gtagatacag
    22021 ctgatgtgga tgggacacaa gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg
    22081 gagtgaaaga aaatacaaaa gacagcatcg atgttcctgc tgcttatctt gaaaaagctg
    22141 aagggaaagg tcctttcact gccggtgtaa accaagtaat tccttatgaa ctattcgctg
    22201 gtgatggtat gttaactcgt ctattactaa aagcttcgga taatgctcct tggtctgaca
    22261 atggtactgc taaaaatcct gctttacctc ctcttgaagg attaacaaaa gggaaatact
    22321 tctatgaagt agacttaaat ggcaatactg ttggtaaaca aggtcaagct ttaattgatc
    22381 aacttcgcgc taatggtact caaacttata aagctactgt taaagtttac ggaaataaag
    22441 acggtaaagc tgacttgact aatctagttg ctactaaaaa tgtagacatc aacatcaatg
    22501 gattagttgc taaagaaaca gttcaaaaag ccgttgcaga caacgttaaa gacagtatcg
    22561 atgttccagc agcctaccta gaaaaagcca agggtgaagg tccattcaca gcaggtgtca
    22621 accatgtgat tccatacgaa ctcttcgcag gtgatggcat gttgactcgt ctcttgctca
    22681 aggcatctga caaggcacca tggtcagata acggcgacgc taaaaaccca gccctatctc
    22741 cactaggcga aaacgtgaag accaaaggtc aatacttcta tcaagtagcc ttggacggaa
    22801 atgtagctgg caaagaaaaa caagcgctca ttgaccagtt ccgagcaaa
//
LOCUS       DBDESG010000034        22762 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.034, whole genome shotgun sequence.
ACCESSION   DBDESG010000034 DBDESG010000000
VERSION     DBDESG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.034"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(5..1234)
                     /locus_tag="ACKB38_001278"
     CDS             complement(5..1234)
                     /locus_tag="ACKB38_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGL7980043.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(1224..2390)
                     /locus_tag="ACKB38_001279"
     CDS             complement(1224..2390)
                     /locus_tag="ACKB38_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGL7980044.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            2534..3547
                     /locus_tag="ACKB38_001280"
     CDS             2534..3547
                     /locus_tag="ACKB38_001280"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGL7980045.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(3592..4011)
                     /locus_tag="ACKB38_001281"
     CDS             complement(3592..4011)
                     /locus_tag="ACKB38_001281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGL7980046.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(4022..5428)
                     /gene="atpD"
                     /locus_tag="ACKB38_001282"
     CDS             complement(4022..5428)
                     /gene="atpD"
                     /locus_tag="ACKB38_001282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGL7980047.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(5514..6392)
                     /locus_tag="ACKB38_001283"
     CDS             complement(5514..6392)
                     /locus_tag="ACKB38_001283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGL7980048.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(6408..7913)
                     /gene="atpA"
                     /locus_tag="ACKB38_001284"
     CDS             complement(6408..7913)
                     /gene="atpA"
                     /locus_tag="ACKB38_001284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGL7980049.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(7928..8464)
                     /locus_tag="ACKB38_001285"
     CDS             complement(7928..8464)
                     /locus_tag="ACKB38_001285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGL7980050.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(8464..8958)
                     /gene="atpF"
                     /locus_tag="ACKB38_001286"
     CDS             complement(8464..8958)
                     /gene="atpF"
                     /locus_tag="ACKB38_001286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGL7980051.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(8972..9688)
                     /gene="atpB"
                     /locus_tag="ACKB38_001287"
     CDS             complement(8972..9688)
                     /gene="atpB"
                     /locus_tag="ACKB38_001287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGL7980052.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(9723..9923)
                     /locus_tag="ACKB38_001288"
     CDS             complement(9723..9923)
                     /locus_tag="ACKB38_001288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGL7980053.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(9998..11004)
                     /locus_tag="ACKB38_001289"
                     /pseudo
     CDS             complement(9998..11004)
                     /locus_tag="ACKB38_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11449..11847)
                     /locus_tag="ACKB38_001290"
     CDS             complement(11449..11847)
                     /locus_tag="ACKB38_001290"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980054.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDCLYIRQRYYYQKNL"
     gene            complement(12355..12837)
                     /gene="greA"
                     /locus_tag="ACKB38_001291"
     CDS             complement(12355..12837)
                     /gene="greA"
                     /locus_tag="ACKB38_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGL7980055.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFVGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(12908..14563)
                     /gene="mltG"
                     /locus_tag="ACKB38_001292"
     CDS             complement(12908..14563)
                     /gene="mltG"
                     /locus_tag="ACKB38_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGL7980056.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(14644..15138)
                     /locus_tag="ACKB38_001293"
     CDS             complement(14644..15138)
                     /locus_tag="ACKB38_001293"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980057.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(15135..15599)
                     /locus_tag="ACKB38_001294"
     CDS             complement(15135..15599)
                     /locus_tag="ACKB38_001294"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980058.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIEFDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(15609..16943)
                     /gene="murC"
                     /locus_tag="ACKB38_001295"
     CDS             complement(15609..16943)
                     /gene="murC"
                     /locus_tag="ACKB38_001295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGL7980059.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(16954..17571)
                     /locus_tag="ACKB38_001296"
     CDS             complement(16954..17571)
                     /locus_tag="ACKB38_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGL7980060.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(17619..20717)
                     /locus_tag="ACKB38_001297"
     CDS             complement(17619..20717)
                     /locus_tag="ACKB38_001297"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGL7980061.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIVQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(20810..21976)
                     /locus_tag="ACKB38_001298"
     CDS             complement(20810..21976)
                     /locus_tag="ACKB38_001298"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGL7980062.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(21984..>22762)
                     /locus_tag="ACKB38_001299"
     CDS             complement(21984..>22762)
                     /locus_tag="ACKB38_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HGL7980063.1"
                     /translation="RWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLM
                     LEFDIEKLAKLAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAG
                     VVVTNSLELYEKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLK
                     DSPAVKEVLYTGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTH
                     ADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQAIEG"
BASE COUNT         6565 a         5055 c         4260 g         6882 t
ORIGIN      
        1 attcttacaa tctctcaaac agttcttgca tttgcacatc atctggaacc agttttaagt
       61 aagtgtgagc atggactttt gcttcttcaa aatgtcccaa ttcacgcaag agatagatat
      121 agtgttccag aaattctgga ttgtccttca aatctcctgt caactcttga taatactcat
      181 aagcagtatc caaatcgtcc atttcttgat aagaacgagc aatcatccac ttggtcaaaa
      241 gattttctgg ctcctcactc tgcaattcta gaatatcctc ataacgctcc tgctccagat
      301 aaatagtggc taaacgaagc aagatttctt ctgtatcctc agcgtcttct tttgcagtaa
      361 ggagataatt ttctgcacca ctagcatcat gcaattcata agaaaattgt gaagcagcta
      421 gcaagaggcg agtttcaaag ggatttttct ctaatccttg cttagcgata cgcagggctt
      481 cttgaacttg atgttcctta tgtaaagcct gactgtaccc atactcatag ccttcaaagt
      541 caggagaaat ggtatcaagc tgcttaaagt agagggtggc tttttgatat tcttcttgat
      601 caaaataaag actggccaac tcaaaagctg ttaagtcatc gtattctaac tccagggctt
      661 tttctaaaaa ctcagtagcc gtttcaaatt tccctaactg agcataggca aagccaattc
      721 gttgataggt ggaaatgccc gtttgctcat aaatcgagcg attatctaac tgggcatagg
      781 cttgaatagc cgcttggtaa ttttccaact cactatccaa ctctgccaaa cccaatatca
      841 agagagaatc ctctgagtag gtcaaggcct ccaataattt ctcacgtgcc acatctgtca
      901 aaccttccag ctggtaaagg tctgccttca gagccaaaga cgagacatac cagtcactgt
      961 cagcttggat ttcctcaaga taggtaaagg cttcttctat ttgaccatcc tcgctagcaa
     1021 ttgcagctag attaagatga acctctggaa aatcctctac aattttcagg taaatttcct
     1081 tggcctgagg atagaaacca atcccttcaa gataagttgc caattcatac agaagatcac
     1141 ttgaatcatt ttctaaagct ttggcgaaat aatgctcagc cttagttaaa tcttgctcct
     1201 ccaaagcctg taacatctgt tgactattgt tcactcttga cttcctcacc ctctaattca
     1261 tatagaccac tgactacctt ataccattcg aaaatggctg aaatgacaac cttagcagag
     1321 gcataaaccg gaataccaag taaaactccc cagataccaa acatagatcc tgaagttaac
     1381 aaaacaaaga gaacattaat agggtggatg tttaattgac ttcccaaaat caatggagag
     1441 acaaaacggc cttcaatagt ttgttctacg ataaagacaa tcactacttt caaaagcatg
     1501 actggaccag caatcaaacc caatactaga gcaggaagca tggctagaaa gctaccaaga
     1561 taagggacca gatttaaaat accagcagta acccccagcg taaccgcata gcgtagacca
     1621 ataatcttga agaagatgat aaacattact gctacaataa tagccactgt cacttgccct
     1681 cgaacatagt tggacaactg ttgattcaca tctgataaaa cttgtccaac aggttccttc
     1741 aattttcttg gaatgaattg ggtcaaatag ttacgcaagc ctttcccatc acgcaagaga
     1801 taaaagagca tgaaaggaac gataatcaag gcaacaatca cttgagaagc cccgctaata
     1861 aaggcactca cccagttgac tgcctgagat gaaaccttac ttgccaaaac tgtagcctgg
     1921 ctagaaaaat tggtcaaaac ttgctctaat tgaggtctga aatcatctgg caggtgctgg
     1981 gctaccaatc cattaacaat cctatctata tcttctaagt aaacaggaac gtttcttgca
     2041 aaggtcaaaa cctgacgttg cagatttgga atggcgactg ccaagcccca aatgataaag
     2101 agagcgatga taacaaagac aatagtgata gctataacac gattaacctt atgcttctcc
     2161 atccaatcaa caataggatt caacaaataa tataacaaac cagacaaaat gactggcaac
     2221 atcacaactg ctaaaaagtc taaaacaggt gaaaatagaa aactaatctt acttaaaata
     2281 aaaagattca gtcccaataa taaggttact aaaaataccg taattgcctt gttatctaaa
     2341 aaccacttga aaaaccaaga taggctaaaa tgtttctctt tttgctccat aaatacttcc
     2401 tttctattgt tctctcatta taccattttt gaaacaaaat aactcttttt taaagtgctt
     2461 acatctaatt ggatatcttt aatttacttg tttttgtgat ataatagtct tatctttaca
     2521 cagaggtata gtaatgaaag aaactgttta ttttggaact tatacacgtc gtacttctca
     2581 agggatttac aaggcagact ttgatacaga aactggtcag ctttcaaatc tagaactttt
     2641 tgcagctgag ccaagtccaa cctaccttgc ctttgaccag caccaacatt tatacactgt
     2701 tggtagccaa gacgataagg ggggaattgc agcctatcaa actgacggga ctgtgttaaa
     2761 tcatgttgtt gaagaaggag ctccccactg ttatgttgct gtcgatgaaa agcgtgattt
     2821 ggtttacgca gctaactatc acaagggaca agtccttgtt tataaacgcc aggaagatgg
     2881 tagtcttcta cttagtgata tggatcaaca cagtggccaa ggtccacatg aaaatcaagc
     2941 ttccccccat gttcactata cagatttaac acctgaccac tatctagtga cctgcgactt
     3001 gggaactgac caagtcatca cctatgacct cgatcaagaa ggaaaattat ctaagctcta
     3061 tacctatcac agcaagccag gagcaggctc acgccatatc attttccata accactataa
     3121 aatcgcttat ctcatttgtg aactcaatag tactatcgaa gttttaatct acgatggcgt
     3181 tggcgaattt gaacgtatgc aggtcatttc aactttaccg gaagcttacg aaggctttaa
     3241 tggaaccgct gctattcatc tctctaaaga cggtaaatac ctctacgctt ctaaccgtgg
     3301 ccatgattct atcgcagtat ataccatcct tgcggacggt agcttagagt tattagaaat
     3361 cgttccaacg catggtcaga ctccacgtga ttttgatttg acacccgacc aaaaatttct
     3421 cattgttgtc catcaagact ctgacaatgc aactgtcttt aaacgtaatt gtgacaatgg
     3481 tcgtctagca gaactctcca acgacttcca tgttcccgaa gcagtctgca tccgttttgc
     3541 tccttaaaac accataaaaa atgaacttgg tattttcaag ttcatttttt cttatagtct
     3601 atttccgaca ttaatacggt taatagcacg ttgcaaagca atcttagcac gacgttcttg
     3661 gtcaatcaaa tgtttgtctt gtgcttcttc aattgcacgt tctgcacgaa gtttggcacg
     3721 ttctgcacga ctgatatcga tatcacgagc acgttctgca gagtcagcga cgattgtgat
     3781 catatcattg gcaatttcaa taacgccacc gtttactgca atccagttca cgtgatcttt
     3841 atcatcgata cgttttacct ttacttcatc aactgctaaa accgcaatca tattttcatg
     3901 tcgtggcaag atccccatct caccatccag agttcgaacc gatacatagc tggcatggtg
     3961 atcatagacg agaccatctg gtgtcacgat ctggacagtt aactgagcca tagatcacct
     4021 cttaaaatcc cattttttca gcttttgcaa tcacatcttc gatagaacct acaccacgga
     4081 aggcatcttc tggcaagtgg tcgtatttac catcaaggat ttccttaaag ccacgtacag
     4141 tttcagcaac tggaacataa gaacctggct gaccagtaaa ttgttccgca acgttgaagt
     4201 tttgtgacaa gaagaactgg atacgacggg cgcgagcaac caaggtcttt tcttcatcag
     4261 aaagctcatc cataccaagg atagcaatga tatcttgcaa ttcatggtaa cgttgaagga
     4321 cacgttttac ttcagcagca actgcatagt gctcttctcc aacgatttca ggtgccaagg
     4381 cacgtgagct tgaagcaagt gggtcaacgg ctgggtagat acccaattgt accaacttac
     4441 gttccaagtt tgttgttgaa tccaagtgag cgaaggctgt tgctggcgct gggtcagtat
     4501 agtcatccgc tggcacatag atagcctgga tagaggttac agaacccttc ttggttgatg
     4561 tgatacgttc ttgcaattga cccatttccg tagcaagtgt tggttggtaa ccaacggctg
     4621 atggcatacg acccaaaagg gcagatactt ctgaaccagc ctgagtgaaa cggaagatat
     4681 tatcgataaa gagaagcacg tcttggcctt ccacatcacg gaagtattca gcgattgtca
     4741 aaccagtaag ggcaacacgc atacgtgctc ctggtggctc attcatctga ccaaagacca
     4801 tggctgtttt ctcgataacg cctgattctt tcatttccca gtaaaggtca ttcccctcac
     4861 gagtacgttc cccaacacca gcaaatactg aaataccacc gtgctcttgg gcaatgttgt
     4921 gaatcaattc ttggattaag acagttttac caactccggc accaccgaaa agtccaactt
     4981 taccaccttt aaggtaaggg gcaagaaggt caataacctt gatccctgtt tcaaggattt
     5041 cagaagaggt agacaactca tcaaaagttg gagctttttt atgaattggc tgacgctctg
     5101 cgtcttctgt aaaaggagct tccaagtcaa tggtatctcc caaaacgttg aagacacgtc
     5161 ccaaagtttc tttacctact ggtacagaga ttggacgacc tgtgtccaat acttccattc
     5221 cacgagtcaa cccatctgtt gattccatgg cgatagtacg aaccatacca tctcctaact
     5281 ccaaggctac ttcaaggacg atttttgttt ttctttcgtc atttttgtag acgacaagtg
     5341 cattgttaat ctcaggaagt ttttcccctg ctgcaaacaa aacgtctaca acgggaccga
     5401 taacctgagc aattttacct gaactcatct ccttctccta ttctatataa gatactgtcg
     5461 gttcctagct caactaggtc ctaagttcat tttcatacga gctggactag agcctattct
     5521 aaggcactag cacctgctac gatttctgta atttcttgtg taatcgccgc ctgtctggca
     5581 cggttatact gaattgtcaa atcattgatg actttcttag cattatctgt cgctgtttgc
     5641 atggctgtca tgcccgcagc attctcagct gtcttggcat cgataatggc accgtaaatc
     5701 atactttctg caaactgagg caacaactgc tccagaattt cttctcggct ggtttccaat
     5761 tcaaaagtca agctgtactc ttcatccgct tcatttggat ccaagtcaac aatcggaagc
     5821 atttgttcca cacgcatttg actggttagc gtattgacat ggtggttgta gcaaacataa
     5881 agctcatcaa agagttcatt ttggtacatt tcaacagttt ttgaaataat cttacgaact
     5941 tgatcaaagc taggttggtc tgacaagcca cgtaattcat aaagtggttg aataccgcga
     6001 gccttaaaga aatcagctcc catcccaccg atacagatca tttcaaaacc tttaccgtct
     6061 gggtggtatt cttctttcaa ctccataaca gctttcaaaa tagaggaatt ataacctcca
     6121 accaaaccgc ggtctgaagt gataacgata tagcctgtct tcttcacaga acggctaatc
     6181 aacatcggat tagttgaagc accagctcca ttaccatgaa ggatatctgt caaaagttta
     6241 cgcactttct gagcgtaaac ttggaagttg cgagcagctt cttcagaacg acctagctta
     6301 gcagccgata ccatttgcat ggcattagtg atttgactcg tattttttgt tgaggcgatt
     6361 tttgttttaa tatcatttag agatactgcc atctgacacc tctattctta ttggaagcta
     6421 gattgattga gaaactctgt aatcgcagca tccaagactg cttcttctgg caagtctttt
     6481 gtatcacgaa tggtttccaa aatctctgga tgttgagcat caaagaaggc atggaactct
     6541 tcctcgaaac gaacaatatc atctactgga acagtatcca agaaaccatg tgtcaaagca
     6601 taaagaatgg ttacttgttt ctcaacaggt aatggtttgt gaacaggttg tttcaagacc
     6661 tcaacggtac gacgtccacg gttcaactta gcctgtgttg ctgcgtccaa gtcagaacca
     6721 aacttagtaa aggcttccaa ctcacggtat gaagcaaggt cgatacgaag tgtaccagca
     6781 accttcttca tggctttgat ttgtgcagaa ccacctacac gagatacaga tgaacccgca
     6841 tcgatggctg gacgaatacc tgcattgaag aggccatcgc caaggaagat ttgtccatca
     6901 gtgatagaaa tcacgttggt tgcgatatag gctgagatat ctcctgcttg tgtctcgata
     6961 aatggtaggg ctgtaattga tccaccacca agttcatcag aaactttagc tgagcgctca
     7021 agcaaacggc tgtggagata gaaaacatcc cctgggaagg cttcacgacc tggaggacga
     7081 cgaagcaaga gcgacagttc acgataagct accgcttgtt ttgatagatc atcgtataca
     7141 atcaaaacat gcttaccttg atacataaat tcttccgcca tagcaacccc agcataagga
     7201 gctaggaaga gcaatggaga tggttgtgaa gcagaggctg tcacaacgat tgtgtagtcc
     7261 aaggcaccgt actgacgaag tgtttctact tgcgtacgaa ctgttgattc tttttgtcca
     7321 atcgcgacgt agatacagat catatcttga tctttttggt tcaagattgt atcaatcgca
     7381 atggttgttt tccctgtctg acggtcaccg ataatcaact cacgttgacc acgaccaatc
     7441 ggtacaaggg cgtcaatagc tttcaaacca gtttgcaatg gttctgaaac agacttacgt
     7501 tgcataacac caggagctgg tgcttctact ggacgagttt tatcagtgtg gatttctcca
     7561 agaccgtcaa ctggacgacc aagcggatcc acaacacgac caatcagact ttcacctaca
     7621 gggacttcca tgattttccc tgtacggcgg attgtatcgc cttcacggat atctgtaaag
     7681 tcacctagga tgataatacc aacgtctgtt gactccaagt tttgagccat accataagag
     7741 ccgttttcaa aattcaacaa ctctccactc atgacatttt caaggccgtg agcacgcgcg
     7801 ataccgtccc cgatataggt tacaacacct gtttcagtca catcaaaatt gggtttgaaa
     7861 ttttcaattt gttgcttaat taaagcgctg atttcttgtg cgttaattgc caaaagaaca
     7921 ccactttcta tttcaaattt tctttaacaa ctttaagttg ttgtttaata ctcacatcaa
     7981 ttgtcttgtg attggcaaaa atgacaaaac caccaatgag actttcatcg atttgttctt
     8041 ttacactcct tactttcaga gacatttttt tctcaatcaa agggagcaaa cgagtcttct
     8101 gttcatcagt tagaggatga gcagacgtaa tcgtcacttc aaatcgattt gtttcttttt
     8161 caagtcggtt caagcaatct acaagcacat cataaaaaag atttgctctg tgattgtagg
     8221 ccagaacctg gataaagttt tgtaataaag gcgacacaga atcttggaaa aaagcaattg
     8281 ttttttcctt atccgactcg tctactgcca cttgttttaa aaaagaaggc agacctgttt
     8341 tttcaacaac ttgcttgatt tgagtcaagt ctgaaaagat acggtcttct tctccttttt
     8401 caagtaccaa ttggacaaaa ggcatgctgt atttttcaat tacctttact gttttcttgt
     8461 ccattaagct tctcctagct gatcgatata ctgatcaatg agtgctttat gggcatgact
     8521 gtcaaggttt tgtgagatga ttttaccagc taagctgatg gtcaaatctg cgacctcacc
     8581 cttaacactc tgtaaagctt ctactttatt ttgagcaatt tcttgattgg ctttttcttt
     8641 taagtgtcct gcttctagtt tagcatctgc taagatatta gccttacttt gctcagctgt
     8701 ttcctttgca ttttcaatga ttgtcttagc ttctttacgg ctaccagcca attcatcttc
     8761 gcgtttttga gccaatactt ctgctttttg acgggcttct tcagctctgt caatatctga
     8821 agcaattttt tcagctcttt cttcgaaaat gcctgtaata ttagaccatg caaatttttt
     8881 aatcaagact agcaaaagaa taaaagagcc agtgattaaa ataaaattac caattaattc
     8941 acctactgtt acgtgcatca gttactcctt tctattcctc ttcaatatta atcttattcc
     9001 ctaaatacac agatgtcaaa agagtaaaaa cataagcttg gatgcaggaa ataaagacag
     9061 aaaatgcagt ccaagccaaa ttagctccaa aggctactgg ataccaataa atagcttggt
     9121 gggaaagaag aagtaacaaa ctcgtcatga cctctcctgc aaagatattc ccaaaaatcc
     9181 gcaaagccaa agataagaag tttgtaaatt cttccaagat attcatcggt atgacaaaaa
     9241 caggactcat aaaagatttt atactttttt taaatccacg acgacgaacg ctttctatat
     9301 gtgtcaacaa aatgacaaga aaagataagg ttaagtcata ctgtaaatta gcggttggcg
     9361 aactccacca gttagtccca tcgatcgttt gaagctttgt cattaagcca aggttattgg
     9421 caatcaccat gaaaaggaat aaacaaagga aaaagagtga gtaatctttc atgtagcgcg
     9481 aaccaatgtt aggttctgta aatccaataa caaagtcata gacatactca agtacatttt
     9541 gctttccttt gggtttcaag gtcatattgc ggcttgccca ataaataaag acaaaaataa
     9601 ctcccacaat caacaaagtc atggctaaca tagtcagatt gaagataaca ggaccaatag
     9661 agatgattgg attaatactt tcttccatct aaaatcctcc cttttcttct cgttccatgt
     9721 atttatttga taataaatga gaagacaaga gttacaaaga aagttccttc aataaaggca
     9781 acacctaaaa acatcaaact acgaaactca gaaagcatat ctggttggcg tgctactgat
     9841 ttaaacagtc cattcatcaa taaaccttca ccgacagata cgcccataca ggcaatacat
     9901 aagcctaaaa atgttaaatt cataacaaat tctcctttaa aataaaattt acttacttag
     9961 tgtagtccta aaatttaaat ttgtcaactt ttaaccgcta ttggaagcgt ttaataaaaa
    10021 ttatttccat tttgctaaat ttcaagttca agttagccat ccagaagtct tctctgggtg
    10081 acttgtagtc caagcatttt ttaggatagt tgttaatcca cttttcgatg aatgcgactt
    10141 ctttgggagt cattttcttg gttcccttag gtaactatct acgaatgagc ctgttgtgat
    10201 tctcattagt tcccctttcc caagaggcat aggggtgcgc ataatagatg tgctcctcag
    10261 aaaatatatc aaacaagcga ttgaattccg ttccattatc tgccgtgatg gaaagaatct
    10321 tgtgttgttt taagatgagt tttagagcct gattgaccac ctcagcactt ttatttggaa
    10381 tcaatcggat gatctgatgt ctactttttc gatccgtcaa gacaatcaag cagtagtttt
    10441 tcgatctcgt aagtagaacc gtatcaatct cataatgccc attctccaag cgaagattga
    10501 tagcttcagg ccgctgttcg atggattgac cagcaggttt aaagttggtg ctagcctgtt
    10561 tcttaagcgc ttttcctttt ctagggtaaa gcaaatcctg cttgcttaac cccaattttc
    10621 catgatgaat ccaatagtaa atggttgaaa ttcccacgtt aaccccttta gccataacca
    10681 tcatttcagg cgaaaatttt tggttatgat agtggagaat cttttccttt agttccttgg
    10741 tcaagcttga tttcttgacc gagcgcttgc gattgttttc ataagactgt tgagcgtagt
    10801 cggcagaata aacctctttg aagcgccctt ttccaagaca ttgtcggact gtcccacgct
    10861 tgatttcagt gtgatagttt gaggagcttt tccaagtaga gaggcaattt ctctatttga
    10921 ttttccttct tttttccatc tttcgattaa gcgacggcta tcgattgtca aatgtttggc
    10981 ttttgtagta taattgtctt gcatctctgt gcctttcttg tgtttgtggt tgaacaacaa
    11041 gtataacaca gaggtgcttt cttatgccta caagagcttt cattatttcc attttctttt
    11101 ggatttcact ctattctgaa aaacttgtgt atatttactg aagctagcaa gtcttacctg
    11161 taaatttaat gaaagcaaca caaaatccga gaggggaatc tcggattaat agatagagag
    11221 tttttagttt aaataaattg tttaaaatat caacaacatc acttcttttc ttaacctgat
    11281 aagtcttgat tcctaatttt ggggctacga ttatattgtc ctcaatatcg tctagaaaga
    11341 cacaatttct aggttataac tggtatttat cgatagttac tcatatacat cagtccacct
    11401 ccatacttat gtgcgagcct ctctttgtat tatacctcca tactcacctt acagattctt
    11461 ttggtaataa tatctttgcc taatgtagag acagtcttgc aaagaaaaaa cttccttgta
    11521 gccatgtttc tgataaaagt ccggtgcctg gaactggtaa gtattgacaa aggcaaaaca
    11581 acaatttcga ttcttagctt cactttctgc ctgttgcaat agttttgaac cgattccttg
    11641 ccctcgcagt tcctctttta caaacaaata ctcgatttct agccaatttc caaaagtctc
    11701 tgctatcaaa cctgccagga gattgccctt ttcatcttcg acataaagat taagtggctc
    11761 actttcagcc tcttctcttt ttgaacggtt ataaacacga atcagattcc ctatttcttg
    11821 cgatttatgt gattccttat tttccaatct aaagtatagt gaaatgaaat aaaacatgcg
    11881 caaatcgatt aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtacta
    11941 ttttaacttc aatgcactat acatctaata ctcaataaaa atcaaagagc aaactaggaa
    12001 actagccgca ggttgctcaa aacactgttt tgaggttgta gatagaactg acgaagtcag
    12061 ctcaaaacat agttttgagg ttgtagatga aactgacgaa gtcggctcaa aacatggttt
    12121 tgaggttgta gatgaaactg acgaagtcag ctcaaaacat agttttgagg ttgtagatga
    12181 aactgacgaa gttagctcaa aacactgttt tgaggttgta gatgaaactg acaaagtcag
    12241 taaccataca tacggcaagg cgacgctgac gtggtttgaa gagattttcg aagagtagca
    12301 aaatggaaaa aggagtgagt gaagcacatc gcctccccac tcctttttct gtttttaggc
    12361 tgttttttca accttcaaga tttttacatc atagctacca acaggcgttt caatggttgc
    12421 tgtatcacct gttttcttgc caatcaaggc ctgcccaatt gggctttcat ttgaaacctt
    12481 acctacaaag gcatctgcac cagctgaacc tacgataata taaacttctt cttcgtcctc
    12541 accaatttct tggatggtga ctgttttacc aatcgctact tcgtcctggg caactgcgtc
    12601 gctattgacg atttcagcat agcggatttt tgtttctaag ctagagattt gtccttcgac
    12661 aaaggcttgt tcatccttag ctgcttcgta ctcactgttt tctgaaaggt caccgtatga
    12721 acgggcaatc ttaatgcgtt ctaccacttc tggtcgacga accaatttca attcttctaa
    12781 ttctttttca agtttttcct tttcctcaag ggtcatagga tatgtttttt ctgccatttt
    12841 tctcaacttt cttctgataa tattttctaa agaaaattat gtgaagtatc acataatttt
    12901 agtttgttta gtttaatttg ctgttgacat gttcagcgac attgcggtcg tggtcttctt
    12961 gattgttagc atagtagacc ttgccttctg tgacatctgc tacaaagtag aggttatcgc
    13021 tcttagtttg attgatgctt gactcaatcg catccagact tggactatcg actggaccag
    13081 gcatgagacc tacattttta taaacattat aaggtgaatc aatgttggta tcaatcgcaa
    13141 catcctcagc tagactgata ttttgcccca gttttccttg ggcatacaag attgcaatat
    13201 tactttgaag tggcatatca cgattcaaac gattgtagaa tacacctgca atgagcttac
    13261 gatcttctgt cttggcacct tctttttcga ccaaggaagc aatggtcaac aactcattga
    13321 cagtcaagtt tttagatttg atagtactat agtaaggaga taggttctta tccatagcag
    13381 ctaacatctc atcaatcaag ctctcaatag ttgtgctttc cttgatagag tatgtagctg
    13441 ggaaaaggta tccttccaaa cgataacgcg caccgctgtc ttttacaggc aaactttcca
    13501 gtaaagtagg atatttcgct actgcttgac tgataaacgt ctcatcttga actttagcca
    13561 agaaagcctc cgctgtcaaa gactctttga agtcaccttg caattgaccc acagcttgag
    13621 caatctgatc caaggtataa ccttctggaa ttgtcaaagt cgcaagtaca ggttcttgcg
    13681 gttcatctgt tccacctttt tgcaactctt tgagtaagtc ttctgtactc atactctttt
    13741 gcaaattgta gtaacctgct ttcaagtcgg tataattttt atacttggca taaaaactaa
    13801 aaatcagacc atgctttacc aaaccagctt tttcaagcgt cgtaccgatt tcttgaacgt
    13861 ttgaaccttc tggaattcca accgtcacat atttcttaga attagcatcg ataggtaata
    13921 aggaatctag cacgtactgg taaccaaagt aaccacctgc tgagagcaag gcaaggaata
    13981 caaggaatga aatcacaaaa gctttcacat gtgacttctt ctctttctta ggcttaacgg
    14041 gttttgctcc ttctctacgg ctacgacgtg atgtatctct gatgatatcc actgtttctg
    14101 tagcaggcgt aggtgtttct ggacctgctt gttctgcttt tttctctgcc gtcgtatagg
    14161 aaacagctac ccttgttggg gtttcattgt attctctttc aagtttctta ggtctaacag
    14221 gacctggacc tggtcttgat ccactttctt ccgctggaga agaaggtaca tcttgacttg
    14281 gatgacttgg aacaccagga gtttctcttt gaatctcatc tgctggagaa gctggtacac
    14341 cttgacttgg gtgagtaggc acggtaggag cttttctcat aatctcctct accgttgaca
    14401 aggaatcagc catgagttct tcagttgaag gttcatttgc aggagtgcga actactgcct
    14461 catcttcttt cagaacttca tcatagcctt ttactttttc taaatctctc agaatctgct
    14521 ctttaaagct taatttctct tcttctcttg acttttcact caaaagtttt tcctccttgt
    14581 tgataatcca taatattata tcttaaaagt ccaaaaaaag caatctatga tacttttcct
    14641 aacttatttt ttcatttccc agaccatatc ataccatgtt tcccctgcaa aggttgactg
    14701 ggactggcct tcgttgacaa atccatgttt ttcatagtag gcgatgagat agtcatgaca
    14761 ggttagatta atgccatctc tctcatcttc aagagcaact tctttcaagg ctgtcagcaa
    14821 tttctggccc agtccgagtc cctgtgcctc cttggcaata gacagacagg tcacagagat
    14881 ataaccacca ggctcatgac tatagtcttt tatttcttct gtaaaagact ggtcttgcag
    14941 atggcggtgc aggccaactg gtccttcgat ataacccatg attcttcctt ctttttcagc
    15001 aaccagaaaa gaggtctgaa tttctctcaa atgtgcttca aagacagaag gaggaatggc
    15061 ttcttcgacc gaaaaatttt caaattcaag ttcaacaatc cgatccaaat cttctaatct
    15121 tgcttgtctg attttcattg ttcctccaga taaaagggat taaaccaaat catactatag
    15181 ccctggctag ttgcatagag caaagttgct tcttcatcaa caaaaccgtt catttcaaaa
    15241 taggaaagca gctcatcagg actctccaaa cgaatccctt tgtaatccag ctcaactgcc
    15301 acctctttca aggctgcaag aagaagtgtt cccaggccct gtctctgatg gtcaaactcg
    15361 atgactaaag aatgtacttt tagacattgc ggattgtctg actggggact tgataaaata
    15421 tagcctaaaa gttgattttc atccctagct agaagaaagg tatccgcaca cttacggata
    15481 ctttcttcta aaatatggga aagttgctgc ttttcagctg gaaaagacga ggtctgaagt
    15541 gcccctatct caggcaaatc agacttgctt gcctgaatga tcttaattgg aatttccatg
    15601 ggaacatcct attgaacatt gcttgtcaag ttagacaaga gacgctcaaa tgagtattca
    15661 taggtttgga tgtctcctgc tcccataaag acgtaaacag cattgtcatg gtctaggagt
    15721 ggagaaacat tttcaacagt aatcacttgg tgttttttgt tgattttgtt ggctaggtct
    15781 tctaccttaa cgtcaccatg atctacttca cgagccgagc cataaatttg cgctagataa
    15841 acagcatctg cttggtttaa agcatgggca aagtcgtcca acaaggcaat ggttcttgta
    15901 aaggtatgcg gttgaaagac tgctacaatt tccttgcttg ggtatttctg acgagccgca
    15961 tccaaggtcg caataatttc tgttggatgg tgggcaaagt catcgataat cactgtatca
    16021 ttgacaattt tctcagtgaa acgacgttta acaccggcaa atgttttcaa gtgctcacgc
    16081 accaagttca aatcaaatcc tgctgtgtaa agaagaccaa taacggctgt cgcattcatg
    16141 atattgtgac gaccaaaggt tggaatgtgg aattgcccca agttttgtcc acggaaatga
    16201 acggtgaagg ttgaaccagt tattgaacga agaagatcac tagctacaaa gtcattgcct
    16261 tcagcttcaa aaccataata ataaattggt gcatcagacg taatcttacg caattcagca
    16321 tcttcaccat agacaaaaag acccttggtg atttgtttgg catagtcgtt aaaggcatta
    16381 aaaacatcct cgagacttgt gaaataatct ggatggtcaa agtcaatgtt ggtgataata
    16441 gagtattctg ggtggtaagg catgaagtga cgctcatatt cgtcagattc aaagacaaaa
    16501 tatttggcat tggccgaacc acgacctgtc ccatctccaa tcaagaagct ggtatctgta
    16561 atgtgagaca agacatgaga caacatacct gtcgttgaag tttttccatg tgctcctgct
    16621 actcccatgc taacaaagtc acgcataaag ctacctagaa actcatggta acgtttatag
    16681 ctgataccat tttggtccgc ataggcaatt tcgacgttgt tatctggacg aaaggcattt
    16741 ccagcgataa tttccatatc accgtctaga tttttttcat caaaaggaag aatggtaatt
    16801 cctgcctgct caagaccgcg ttgggtaaag tagtactttt caacatctga tccctgaacc
    16861 ttgtgcccca tctggtgcaa catcaaggcc aaggcactca tccctgatcc cttaattccg
    16921 ataaaatgat atgtctttga catgttttct cccctattct gtcattctgg tcagattcaa
    16981 ctcttgggca acccgacgtt cttgttctgt ttgtttactt tttttattgt agatttggct
    17041 cttctttaga aaatcataat tgtttttctt tggagcaggt gctgacactt cttcattctt
    17101 ggtagggata gaatgaactt cttccgccaa gatataatga gactgggtca atttttggct
    17161 atatttgaca aattcaccag gattttcctt ttggaaagga gctgtcggtt gattgccctg
    17221 tctaactaga ctgggctgag aatgacgtct cgcaaggctg aaatcctgag ttaggtagtt
    17281 agcagagcgt ttctttttca agtccgcacg cgcttcttca cgcgccacct ccgcatagct
    17341 ctttccttct tttttaaccc ctaaaggagc ctttttaggt ttttcgactt gcttttcaat
    17401 cggttttact ggtttttctt cagcaatagg agcccattct aaataatttt tatctcgata
    17461 ctcacccttg atattactga tcagatcaga ctcatcatag agattcatga ctggcatttc
    17521 agtcaacatg acctcgtcat ctgacaccaa tggaaatcgt tcttgtttca ttttctattt
    17581 cctttcaaca cttcattata gcgtattgtc ttgatttttc aagtgctggc ttcagaaatt
    17641 cccaaaattt ctctaatttc tgctagggtc agactaccac gtgactctgt gccgtccaat
    17701 acttgtgaca ccagatgttt cttttgttct tggagttcct gaattttttc ttcaatggtt
    17761 cccttggtca ccaagcgata gacctcaacc gtttcttcct gacccatccg atgggcacgg
    17821 ccaatggctt gcgcttccac cgcaggattc caccaaaggt caaccaagat cactgtatct
    17881 gcacctgtca ggttcagacc gaccccacca gccttgaggg aaatcagaaa ggcatctctt
    17941 tctccttggt taaaggcctt ggtcatgtct tgtctttcct tggctggggt tgaacccgta
    18001 attttaaagg aagtcaggcc caagtctggc agttcttgtt caattttttc caacattccc
    18061 ttgaactgag agaaaatcaa gacacggtgt ccgccgtctg ccacctgtac cagtaggtct
    18121 cggagactat ctagtttgcc gctggctccc tgataatctt ccataaacag ggcaggagtg
    18181 tcacatattt gacgcaagcg catcaaacca gataaaattt ccacacgact tcgctgaaat
    18241 tcctgttctg acacttgagc cagatggtct cgcatctgtt gtaactgggc aaggtaaata
    18301 gccttttgct ggtcttccag ttcattttta taaaccacct caatcaagtc tggcaattca
    18361 gtcagaactt cttctttctt gcgtcgcatc acgaaaggct tgataaactg agccactcgc
    18421 tctgctggca atttcataaa ttctttcttg cttggcaaaa gtccaggcat gacgatttgg
    18481 aaaatagacc acaactcacc cagatggttt tcaatcggag ttcctgacaa ggcaaagacc
    18541 gacggcacca caaattgtct caaggtctgg gcaatcttgg tctgggcatt tttcatgacc
    18601 tgagcctcat ctaagaaaag gaagtcaaag gccatccctt gataaaactc actgtcctga
    18661 cggaaggtgg catagctagt cacatagatt tgatggctct cggcaagaat ctcctcacga
    18721 cttgctttca aaccatgaac aacagtcaca tccaactgtg gagcaaattt ctgaaactca
    18781 tctgcccagt tgtaaatcaa acccgacgga gcgagaatca aaacccgact ttcttttgtc
    18841 acttgactag tcaaaaaagc aatggtctga agggttttcc caagtcccat atcatcagcc
    18901 aaaatcccac caaaaccata atgatggagc atctgcaacc agccaattcc cttttcctga
    18961 taatctcgca agtcagcctt gacctgagtt gcttgcaaag gaaagtcctc tggatgcgtc
    19021 aaatcctggg ccagattctg gaattcttgt gaaaaagaaa cacggtctcg cccttcaaag
    19081 agatgagcta aactgtaggc caaggatttc cgagcctgca aggtcccatc ttttaattca
    19141 aattgcccca gttcctgtag attttggcga attttcttgg tttcttcatc gaaaaagtaa
    19201 acttgattag acgaatcaat ataaaaatcc tgattggcaa ccaaggcctg catggcttgg
    19261 tcgatttcct cctggacaat attttgaaaa tcaaactgga tttccaagag acctcccttg
    19321 gaggcaatct gcacctgagg actcgctagg ctataaagct cttctagttt atctgatagg
    19381 tcaacatgcc cgagtttttc aaagactgga atgatatcat gaaaaaaatg atagacagac
    19441 tccgctttta aggcctgacg ccaagattga aaatcggcct caaagcccgc agccaaacag
    19501 acttggaaaa ttctttcttc taagtctgcg tcacttgaaa agggtaattc ttctagctct
    19561 tgtcggctag atacctgtct atttccataa tcaaactgaa tttctaaacg aatccgatta
    19621 tcttcttccc tgtcaaagta aaaagagggc gcaaaagttt tgatttgtag acgttctgga
    19681 gctgaaacgg tgcccatctg gataaaaaga gtcagacagg aggccaattt gtctcgatca
    19741 ctgctatcaa attgcaggta tttctttcct tgttgaccca caggtaacgc tttaatttcc
    19801 ttgagaagac gcatctgctg gtctgttaaa aaataaacct gacctttatg gaaaagtact
    19861 gctccctgat aaaagacatt gaccctagga ctctcactga tttccatttc aaaataatcc
    19921 gagtattctg ttactgtaaa ggcaaataga ttggcatcag catgcatatc ctgaaaaagc
    19981 agggtttggt agctatccac ttgatggtca aattgaaaat ggggcaaggc catcagtaaa
    20041 ttcacaccct gctcaaaaaa ggtcagaggg aaaaagaggt gccgaccttg gttttggaaa
    20101 aagaggtctg gaaccagccc ttcctccgtt agtccgtgca agaaagtcaa aagttcttgg
    20161 ctggcatcat caaaggcttc ccaagaaaga gactcctcat aaatcttgcc aatcatatac
    20221 gactttctct gctcgacaat ccttaaaaaa agtggaatat cccgaatgac atagtatttt
    20281 tggctattga tttggccgat tctcagagtc cacaagatat gattggttcc tgcttccacc
    20341 tgacccacag ctgataactc ataggcgcat tctgattttg gagataaaat tcgatccaaa
    20401 aacttgccac ccaaggtcac cttggtttca acagcctctt tttcttcatg accttcttcc
    20461 agactccaca agatttcctg accacgctca tcatttttca gaaaatgctc tagcgctgcc
    20521 aaatgcacac agtagcccct cttttgaaaa aaatcacagg cacaaaaaac caaatcatcc
    20581 tctaaactat agcgcagttc ttcttctgca acgcgagcgt agagccgatt gttcttttcc
    20641 ttgatgatat caaccttacc agtttcataa agggcaacac cttcgatacg aattttcccc
    20701 ggaatcaatt tagccatatt ttcaccttta ccttatcttt ttattatacc atattttcgc
    20761 ctatgaaaat agccttctag gaagactttt ctcctagaag gctggatttt taacgtttgg
    20821 caaaagtagc cacaatccgc tgacagactt cttgcaacag agatttgggc atagctatat
    20881 tgatgcgggc atggagactt ccttcctctc caaaatccaa accacggttg aggataacct
    20941 tggcttcatt tctcaacaac tcttgcaatg tttcatcagt caggtcataa gctgaaaagt
    21001 caagccaaat caagtaggta ccttgcggtt tcatgacctt gattttagtc tcttttccaa
    21061 atagatccat cacataattg atgtggtctt caaagacttg cttgagttcc tctagccaat
    21121 ctttaccgta tcgataggca gcttctgtcg ccaaataacc caagcctgaa atttcatgct
    21181 gattattggc caacaggcgt ttctggaaag ccagtctcaa cttaggattt tcaatgactg
    21241 cataggaatt ttttgttcca gcaatattaa atgttttagt ggcactgctc aagacgatag
    21301 caaaattttt gaaggcagga ttgatggtat tgaaagactg gtgtttgtga ccaaagaggg
    21361 tcaaatcttg gtgaatctca tccgaaacta acaaaacacc gtgtttttgg cagagttggc
    21421 caatcttctc caacacttct ttttcccaaa cacgtccacc aggattgtga gggttgcaaa
    21481 gaacatagag tttaacctcc tcttccacca aatccttttc aagttggtca aagtcaatct
    21541 caaacagact atccttttcc actaaggaat tagtaatcaa tctacgatta ttcaacttga
    21601 cactgcgagc aaagggtggg tagacaggcg tgttaattaa aaccgcctcg ccttcttttg
    21661 taaaggtttg aatagctgtt gagatggctg gtaccacacc ctcgataaag acaagagcct
    21721 ctttgtcaaa gttgtaaccg tattgtgtag cttcccactt ttgaacttcc ttaattaagt
    21781 cttcactggc ataggtataa ccataaacca gttggtctgc gtaagtttgc acggcttggc
    21841 ggatttcagg caagaccaca aagtccatat ccgctatcca agctggtaga acttcactat
    21901 ccgtttctgt ttctttccat ttataggtat ggtgccctaa acggttgggc aggcttgtaa
    21961 aatcatattt tcccatcttt gtcttatcct tctatggctt ggcgcaaatc tgcaatcaaa
    22021 tctctagcat cctcaatccc aatagacaaa cgcaagaggt catctgtcaa accataagaa
    22081 tggcgtacct ctgctggaat atcagcatga gtttgagtcg ttggataagt aataagactt
    22141 tccactccac ccaaactttc cgcaaaagag aagaccttga gactgttcaa aatatgagga
    22201 atgcgtgttt catcggctac tttaaaggaa atcatgcctc cacgaccagt gtagagaact
    22261 tccttaactg ctggagaatc cttcaaaaag gcaaccactt cttgggcgtt agctgttgag
    22321 cgctccatac gaagagacaa ggtcttgaga ccacgaagca actggtagct gtcaaatgga
    22381 gacaagactg cccctgttgt attaagattg taaaaaagct tctcgtatag ttctaaacta
    22441 ttggtcacaa ccactccagc caagacatca ttgtggcctg ctagatactt ggttgctgaa
    22501 tggagaacga tatctgctcc atcttcaatc ggacgttggt agatagggct atagaaggta
    22561 ttgtccacca ccactttggc acccttagca tgagccaatt ttgctagttt ttcgatatca
    22621 aattccaaca tcaagggatt ggttggggtt tcgatataga gaacatccac atccttttct
    22681 aactcggcaa tcaactcttc ttctgtattg gcataggtaa aatggaaatg accttcctgc
    22741 tccacttggt taaaccagcg aa
//
LOCUS       DBDESG010000035        22036 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.035, whole genome shotgun sequence.
ACCESSION   DBDESG010000035 DBDESG010000000
VERSION     DBDESG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.035"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(48..1937)
                     /locus_tag="ACKB38_001300"
     CDS             complement(48..1937)
                     /locus_tag="ACKB38_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGL7980064.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSTFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2075..3640
                     /locus_tag="ACKB38_001301"
     CDS             2075..3640
                     /locus_tag="ACKB38_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGL7980065.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3640..4380
                     /locus_tag="ACKB38_001302"
     CDS             3640..4380
                     /locus_tag="ACKB38_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7980066.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4489..4704)
                     /locus_tag="ACKB38_001303"
     CDS             complement(4489..4704)
                     /locus_tag="ACKB38_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980067.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5439..6689)
                     /gene="ilvA"
                     /locus_tag="ACKB38_001304"
     CDS             complement(5439..6689)
                     /gene="ilvA"
                     /locus_tag="ACKB38_001304"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGL7980068.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6739..7095)
                     /locus_tag="ACKB38_001305"
     CDS             complement(6739..7095)
                     /locus_tag="ACKB38_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGL7980069.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7333..7569)
                     /locus_tag="ACKB38_001306"
     CDS             complement(7333..7569)
                     /locus_tag="ACKB38_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980070.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7676..8698)
                     /gene="ilvC"
                     /locus_tag="ACKB38_001307"
     CDS             complement(7676..8698)
                     /gene="ilvC"
                     /locus_tag="ACKB38_001307"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGL7980071.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8764..9240)
                     /gene="ilvN"
                     /locus_tag="ACKB38_001308"
     CDS             complement(8764..9240)
                     /gene="ilvN"
                     /locus_tag="ACKB38_001308"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGL7980072.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDIAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9233..10933)
                     /locus_tag="ACKB38_001309"
     CDS             complement(9233..10933)
                     /locus_tag="ACKB38_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGL7980073.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11133..12800)
                     /locus_tag="ACKB38_001310"
     CDS             complement(11133..12800)
                     /locus_tag="ACKB38_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGL7980074.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12803..13168)
                     /locus_tag="ACKB38_001311"
     CDS             complement(12803..13168)
                     /locus_tag="ACKB38_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGL7980075.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13325..13513)
                     /gene="rpmB"
                     /locus_tag="ACKB38_001312"
     CDS             complement(13325..13513)
                     /gene="rpmB"
                     /locus_tag="ACKB38_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGL7980076.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13629..13805)
                     /locus_tag="ACKB38_001313"
     CDS             complement(13629..13805)
                     /locus_tag="ACKB38_001313"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGL7980077.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(13916..14113)
                     /locus_tag="ACKB38_001314"
     CDS             complement(13916..14113)
                     /locus_tag="ACKB38_001314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980078.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(14396..15940)
                     /locus_tag="ACKB38_001315"
     CDS             complement(14396..15940)
                     /locus_tag="ACKB38_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGL7980079.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16153..16455
                     /gene="gatC"
                     /locus_tag="ACKB38_001316"
     CDS             16153..16455
                     /gene="gatC"
                     /locus_tag="ACKB38_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGL7980080.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            16455..17921
                     /gene="gatA"
                     /locus_tag="ACKB38_001317"
     CDS             16455..17921
                     /gene="gatA"
                     /locus_tag="ACKB38_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGL7980081.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            17921..19363
                     /gene="gatB"
                     /locus_tag="ACKB38_001318"
     CDS             17921..19363
                     /gene="gatB"
                     /locus_tag="ACKB38_001318"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGL7980082.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19492..20052
                     /gene="efp"
                     /locus_tag="ACKB38_001319"
     CDS             19492..20052
                     /gene="efp"
                     /locus_tag="ACKB38_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGL7980083.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20074..20463
                     /locus_tag="ACKB38_001320"
     CDS             20074..20463
                     /locus_tag="ACKB38_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGL7980084.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20456..20878
                     /gene="nusB"
                     /locus_tag="ACKB38_001321"
     CDS             20456..20878
                     /gene="nusB"
                     /locus_tag="ACKB38_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGL7980085.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(20913..22036)
                     /locus_tag="ACKB38_001322"
                     /pseudo
     CDS             complement(20913..22036)
                     /locus_tag="ACKB38_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6488 a         4983 c         4066 g         6499 t
ORIGIN      
        1 ttctatcttc cattatatca tactttttca gtctatggta gctctcttta aaaggcaccg
       61 atactgccgc cacctccgcc tccagagaag ccaccaccag aacttccact tccagaagat
      121 acagagtagg tgcttgctgt atttgcgaca ctagcataat ggctcatttg tgctgttgaa
      181 tgataaaacg tactgtgcca gccataagct acatagagat tgatatctgg attttccact
      241 tgaatctgat gaaccttcat caaatgacta accttgtccg catagccaaa taaggtcgca
      301 tagaccaaga ggcgattcca gaccacaata ctttccagtt cagcctgatc caatcgtgca
      361 atctcacgca acatattttc aaaactggtc cagagatagt agacctcagc tcccgcttca
      421 tttagaacac catcacgatt atctagtcga agcttccaat aatagaaaac agacaaaact
      481 aaccctagaa aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg
      541 tacaagaaca aaccaaatcc gataaatagg ggcaggatag tcaaggcacc catacccact
      601 tgcaaggcct tttccccacc agttaaagga cgataataat ctgggagccc ccagaaggaa
      661 actcgttttc tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagt
      721 tgaagccctc ttgcttgaat ccgtttttca tcagaaactt tggctctacg ataaagacta
      781 tcagatacct tgtaatccgc aaacaaattg gaaagagttt cttctttttt acctgaaaaa
      841 gctagattta ggcagtcttt ctcaaagctt gacaaaccat cttcttttac tagcctcaaa
      901 ccaactgcat ctccttctga aatgatagag acattcccac ggtctatcac atctagcaag
      961 gtagcttgaa taagttgatc aaaggtgaat tttccagctc ccttgaccaa gggactcact
     1021 tcctccaagg aggtcgagta gactgcttct gataaaacca taggctctaa ttccattggt
     1081 ggttcataga gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa
     1141 ataaaataga agcagacact caataacaag gagatggaaa ggatcgaagg gaggacccaa
     1201 gtaacgagtt gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac
     1261 tcttctaaac gattcccttt caatccctga tccctagcgc tagcaaaatc ggtccgaggc
     1321 caataggcat gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa
     1381 tcaaggttac tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt
     1441 ttttcagccc ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta
     1501 tctgtcagag gttgccaatt taattcagcg atatcatcat aaaggaaaag taaatttttt
     1561 aagttccaga cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc
     1621 acagtataac catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg
     1681 tttttcgcgg cctgaatctt tggatgaggg tcaatgtcaa acccgctagg catcttacca
     1741 gcacgtccaa gtcccacgat ttggccctta aagtcctcct caaactggta aactatcttc
     1801 tgtctaaact ctgccgtatt gtctgcatga atatacaaat ccccttgata agagtttatc
     1861 ttgaaatcaa tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag
     1921 aaaaaagttt ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt
     1981 ttcgtaagta aagacttact tatgttgaaa aattacgcat ttttcgataa aataggagac
     2041 agttattttt taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt
     2101 cacaagtctc ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg
     2161 catcggtatg gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg
     2221 agctgtcaaa atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa
     2281 gaaaatcgct aaggacttag gtaaagaacc tttggttgtt aaaaccaagt gggaaggtct
     2341 agtccctgcc cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc
     2401 agaacgcaaa caagaaattg ccttttcgag cagttactat actagcgaac cagttttgct
     2461 tgtcaaaaaa gattctgcct acgcaagtgc taaatctttg gatgacttta acggtgcaaa
     2521 aatcacttct caacaagggg tctaccttta taacttgatt gcacaaatcc caggtgctaa
     2581 aaaagaaaca gccatgggag acttcgctca aatgcgacaa gctcttgagg ctggtgtcat
     2641 tgatgcttat gtttctgaac gtccagaagc actgactgct gaagctgcga actctaagtt
     2701 caagatgatt caagtagaac ctggtttcaa aactggggaa gaagatacag ctatcgctat
     2761 cgggcttcgt aaaaatgaca atcgtattag ccaaatcaat gccagcattg aaaccatttc
     2821 aaaagatgac caagttgcct tgatggatcg tatgatcaag gaacaacctg ccgaagctac
     2881 aacaactgaa gagactagca gtagtttctt tagccaagtt gctaaaattc tttctgaaaa
     2941 ctggcaacaa ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat
     3001 cataggtctc attattggac ttgccattgg tgtcttccgt actgctcctc tctctgaaaa
     3061 caaagtcatt tacggcctac aaaaactagt cggctgggtt ctcaatgtct acattgaaat
     3121 tttccgtggt acgccaatga ttgttcaatc gatggttatc tactatggaa ctgcccaagc
     3181 tttcgggatc aaccttgacc gtacactggc tgctatcttc atcgtttcaa tcaataccgg
     3241 tgcctacatg actgaaatcg tccgtggtgg tatcctagca gttgacaagg gacaatttga
     3301 agctgcgact gctcttggta tgacccataa ccagaccatg cgtaagattg tcctacctca
     3361 ggtagtccgt aacatcctac ctgcaactgg taatgaattt gtcatcaata tcaaagatac
     3421 atctgtattg aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac
     3481 acaaacctat caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac
     3541 cttcaccgta acacgtatcc tacgctttat cgagcgcaga atggacatgg atacctacac
     3601 tacaggtgct aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa
     3661 attaaacacc tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttcactcact
     3721 gtccacaagg gagaggtcat ctctatcatc ggaagctctg gaagcggaaa atcgaccttc
     3781 ctacgctcca ttaacctact tgaaacacca actgatggac aaatccttta tcatggacaa
     3841 aacgtcctcg aaaaaggcta tgacctcacg caataccgtg aaaagttggg gatggttttc
     3901 caatccttta acctctttga aaatctcaat gttcttgaaa acacaatcgt cgctcagaca
     3961 actgtcctaa aacgcgaacg cacagaagct gaaaagattg ccaaagaaaa cctggaaaag
     4021 gtcggcatgg gagaacgcta ctggcaagcc aaaccaaaac aactctcagg tggtcaaaaa
     4081 caacgtgtgg ccatcgctcg tgccctctcc atgaatccgg acgctattct ctttgatgaa
     4141 ccaacatcag ctctcgatcc agaaatggtt ggagaagtcc tcaaaatcat gcaggacctg
     4201 gctcaggaag gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc
     4261 tctcaccgtg ttatctttat ggataagggc gtgatcgctg aagaaggtaa accagaagac
     4321 ctcttcacca atcctaaaga agaccgaaca aaagagttcc ttcaacgcta tctcaaataa
     4381 aaagaaaagg ctgcatcaac cgtgcagtct ttttgctgtc ctcaaaatga aaaggcagat
     4441 tctctatctc tactacttga aggtcaagaa tccgccgtta tccaaagatt aatcttcaaa
     4501 tttttcataa tttttttcat agaaatcaat taggcctagg gctgtttcaa atgaaatatt
     4561 ttttactttt gcacgaccct gcgccagagc aatgatagac atttcacgcg cattcgtttc
     4621 tttagagata cggtagcctg tgattttctt atcacgaacc cagccaacaa ctgattctac
     4681 tttttcaaag tttgacttag ccatgttttt ctcctatttt cttctttata atatttaatt
     4741 gtatacgttt ttactacttt tgtcaataaa aaaaacatat attcaacgaa aataattagt
     4801 tcagaatttt cttacttcct ttttaaatcc gataatatat aggttttgct agcctctaca
     4861 ccataaatat ttgctaataa aaatactgta ttattaccct cttaaggtgc gaaattattc
     4921 aagttcttaa aaaatgcttc tctatttccg taagatagct tatttcttcg atttaagaac
     4981 tgaagatgat agttctaacc tttagaaatc caagccttca ctagctcaaa aactcctatc
     5041 tttcttcctc aataaatcat ctgagagata ggtattcctt atgtccttca aattcatcac
     5101 tcctttggat attgaggtgc tagcctagaa gcagacaccc gattatctaa accatcctta
     5161 atagagatga caagacagat aagctcagtt aattacttat atatcttttt aatatagtat
     5221 atttgtcaac aaaaagaaat caaatcatac cttatactca atgaaaatca aagagcaaac
     5281 taggaagcta gccgcaagtt gctcaaaaca ctgttttgag gttgcagata gagctaacgt
     5341 ggtttgaaga gattttcgaa gagtattaaa gacagtgtga ctatgcttgt caatagatag
     5401 gaaatcaaaa tgaaggtatg atattttttt attagtcctc agacaagcat attataaagc
     5461 gtttcattac catttaagtt aatataagct ggatcaaaac cttccattct acgaatcaaa
     5521 cctgcataat catgcttatc tgctaaagcg atcccaatta atactgggcc tgttcccttg
     5581 ctagctcgtt tgatatactc aaaacgtgtg atatcatcat ttggccccag gatatcattt
     5641 acaaactcac gcaaagctcc tggacgttgt gggaaattga ccacaaagta atgtttgata
     5701 ccatcataaa tcaaggcacg ctcttccatt tctggcatac ggttgatatc attatttcct
     5761 ccagaaatga tacaacaaat ggttttcccc ttaatatatt cagctaaaac ctctaaagag
     5821 gcgatactag ccgctccagc aggttctgcg actatccctt gcttagagta aaggtcaatc
     5881 aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag ttttaatatg ttgacgagtt
     5941 gcttcatagg tcaactgacc taccttttgc acagcaatcc catcagcaaa tttatcaatt
     6001 tccttgagtt ttactggacc tccagcctca aaggcagctt tcatggaacg cgctccattc
     6061 gcctctactc cgataacctc aatctctgga cttgtttcct tgatataggt agaaaccccg
     6121 gcaatgagac cgccaccacc aacaggaacc aagacagcat caaaatcaat cgattctttt
     6181 cgagcttctt ctaaaatctc ataagcaact gttccttgac ctgcttgaac atgagcatca
     6241 tcaaaaggat caataaaggt acgattttca gagactgtaa attcttgagc tgctttggct
     6301 gaggcatcaa aggtatctcc aactagttta atagttacaa aatccccacc aaaaaagcga
     6361 acctgaccaa tcttttgttg tggcgtagta atgggcataa agatagtagc aggaattttc
     6421 atttcattac aagtataggc tactccctgc gcatgatttc ccgcagaagc gcagactacc
     6481 ccacgttcac gttcttcctt gctgagctgg gaaatggcat aataggcacc acgaatttta
     6541 aaggagcgaa cacgctgggc attttctttt ttcaaataaa tcttagcacc atacttctcc
     6601 gataaataat gatcgtaatc cagtggagta ttcacaacca caccgttcaa gaccttgtga
     6661 gccttgatta tatcttttga acttaacata taaaacctct ttttctataa aaaacagtca
     6721 ctaatgactg ttttttagtt attcatctcc gaaaacttca gtaacacttt catggtattt
     6781 atgagccata cgaatcgaaa actctttagg aagttcataa cgatttgtgg aaatccaatc
     6841 atataaccat tctaccaaac tttcttcact atcattacga tggaagataa aatagtttaa
     6901 aatttcttta tattctacat cattcaaatc caacgtgtct acaaattgac tacgaggaac
     6961 aaaattagaa tctaattttc ttagttgatc tgtcaattct cccaattgta aatccaactg
     7021 aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat cctgtcttga tagaaaacaa
     7081 attggtaata ttcatatgat ttcaacccct aaataagtac atctaatatc ccacgaatag
     7141 ctgaagctaa agttttcgca gtccagtcat ttaaacccga ttctttcaat ccagatgcaa
     7201 taatttgctc tatttgatat aaaccgccat ctatcgtctc atttattctg cgaagaggac
     7261 cttcaatata ggataaagct tctccgaatc taacagctga tttaggtcct aatgatttac
     7321 caactatatt tgttaatgtt ttttatttct caaaatccat ttcgctatat aacgagcaga
     7381 tttagtcgca ccagctctct caaacggaga atgaatttca agaccttctt ctctagctat
     7441 ttgtgccaat ttgaagaatt gttcaaagtt catatgcaac ctaccatctt caaaattagc
     7501 atcatcataa atgttaatta cagcattttc atcatacgac caatctgctt tattttggtc
     7561 tgctagcact attaccgacg gtgtaattac attatacaac tagaaccaat aataacgatg
     7621 caatttttct cataatcttt ttcataaaat catctccagc gctatatatt tctaattagt
     7681 tatagatttt gaatgcatca tcgtcgtttt taccaacgaa tggcattgct ttacgcaatt
     7741 ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc acggtaagca gtcaattttg
     7801 gacgtccagc tttatagtca tttacaaagt catttgcaaa tttaccattt tggatgtctg
     7861 ccaagacagc cttcatattt tctttaactt gttcagtgat tacacgtgga cctgatacat
     7921 agtcaccgta ttcagcagtg tttgaaatag attgacgcat tttcttgaat ccaccttcgt
     7981 agatcaagtc aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc aattctggag
     8041 cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc aaaccaccac
     8101 aaagtacagc ttgttcacca aacaaatctt cttcagtttc ttctttgtaa gttgtttcaa
     8161 gaagacctac acgagccgct ccaacacctt tacaccagtc catagcaatg tttttagcat
     8221 ttcctgttgc atcttggtat actgcataaa gagctggaac accaaatcct tcttcgtaag
     8281 tacgacgtac caagtgtcct ggtcctttag gagcacacat gaagacatct acatccgcag
     8341 gaactttgat aaattcaaag tggatgttga aaccatgggc aaatccaact gcgtttccag
     8401 cttccaagtt tggagcgatt tctgcttcgt acaattcttg ttgaatttcg tctggcgcca
     8461 agatcatgat aacatcagcc aacttagtag cttctgctac tgtgtaagta tcaaatccat
     8521 cttcttttgc tttatcaaaa gatttacctg gacgtacacc gataataacg tcacgacctg
     8581 aatcacgcaa gttttgagca tgcgcatgcc cttgtgaacc ataaccgata acggcgattt
     8641 ttttaccgtc aagtgctgct actttaacat ctttttcata ttccatttga actgtcatag
     8701 cttttctctc ttttctatta tttattgcct tttaggctgg tttaataaat ttaagttgga
     8761 tttttaatcg cgggtaaatc cagttgcacc cgttcgagca atattgcgaa taccgtatgg
     8821 gcgaatgact cgcaataggg cttcgctctt ttctgcattt cccgtcatct gaatggtaat
     8881 cgagcttggc gctatgtcta ctactgttgc acggaaaggt tgaataatcg ctaaaatctc
     8941 agctctcttc tcagctggcg ctgacatctt aaccaaaatc acctcgcgct ccaaatgagg
     9001 cttgtctgta atatctcgaa tgcgaatcac atcaatctga cgattgagct gtttgatgat
     9061 ttgctccact tcatcatgag aagcaacatc aataataata gtgatacgcg atacattcgg
     9121 atcttctgtt gctccaacag agatgctttc aatattaacc tgacgacgag ataggacacc
     9181 tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca ttctacgcat
     9241 ggaactgcac ccccaacatc tcatgattac tcttaccagc cggtaccatt ggtaacacct
     9301 gttccttacg agaaatatct acctcaatta gcataggaac atcctcagtg atgacttcaa
     9361 ggtcttgagc caaggtctca ggattgtcaa acttatagtt tttaatacca taagcctgcg
     9421 ccatcaattg gaaatcagga agggtatcaa agaccgactc tgatgttctg ccttcataga
     9481 aggattcctg ccactggcga accattccaa gtgaatgatt gttcagcata accaccttga
     9541 ttggcacctt gtaaatattc aaaatagcca actcctggtt ggtcatttgg aaaccaccat
     9601 ccccaacaaa caagactact tccttatctg ggttagcaat tttagcaccg attgctgctg
     9661 gaattccaaa gcccattgtt cccaaaccac ctgaagtcac taactgacgt tcattttggt
     9721 agggataata ctgagctgtc cacatttggt gttgaccaac gtctgttacc acaatggcat
     9781 ctccattcgt caattcacca attcgttcaa taactgcttg cggttgaacc acacgctctt
     9841 tcttatcata agaacgaaca cgattcttgt ctttagtgac tttctcaatc cacttttcag
     9901 tgttgttgtg aactgttggt tctgctagca acatttgcaa ggccttctta gcatctccaa
     9961 ctacaggaat gtctgcactg ataatcttgc caatctcagc tgggtcaata tcaatgtggg
    10021 caaccttagc attcttagcg aaagtcttag gattccccgt caaacggtca tcgaaacgag
    10081 aaccaatact aatcataaag tccgcttccg tcatagcaat atttgctgcg aatgacccgt
    10141 gcatgcctcc cattccaaga aagagtgggt gactcgttgc aatcgttcct tgtcccaaaa
    10201 gactggttac cactggaatt tgatagcgtt ctgcaaattc atttagttcc gtagcagcct
    10261 cagcataact aattccacca ccagctaaca agactggctt tttagccttg gacaattgct
    10321 tcaagatttt cttgatttgc atatcattcg gctcaagagt cggctgataa cttggtaaat
    10381 tcacttctgg tgaataaatg aagtctgttt ctaaagcaga tatgtctttt ggtaggtcaa
    10441 ttacaactgg ccctggacgg cctgtagttg cgatatggac agcttccgta atgatacgcg
    10501 gaatatcagc tgtctcacga acttggtaat tgtacttagt gattggcatg gtaattccca
    10561 cgatgtctgc ctcctgaaag gcatccttcc caatccctgc tcgcgccacc tgacctgtaa
    10621 agaccaaaag gggaacgcta tcgctcatgg catccgcaat ccctgtaatg gcatttgttg
    10681 ctcctggtcc actagtgacg acggcaacac ccaactttcc agttgatttg gcataacctt
    10741 cagcttcatg caaacaacct tgctcatggc gccctagaat gtggcgaatg cctttaaaat
    10801 tatatatcgc atcataaaaa ggcaaaaccg caccaccagg ataaccaaag atggtatcaa
    10861 ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga gattctaaac
    10921 tgattttctc cattattccc ctttctttcc tcttaaaaat aacttgttac tatcatacca
    10981 ttttttcaaa atttttcaag ataaaagaag aaattttctg aattttctat tttaacgttt
    11041 atttatgaat gttatttttg tttttattaa aaagataccg atttcattta ctaaaaagaa
    11101 aaaagaactg atttctcagt ccttcattaa tcttattcca cactaaatag gtatgggtaa
    11161 acaggttgtt gaccttggtg aatctcgact tcaacgtctt cgaattcttc tacgatttct
    11221 tgagcgattt cattggcaag ttcttcgctt ccgtcttcac ctacatagaa ggttacgatt
    11281 tcactgtctt catccaacat atgtttcaag gtttcagtca atgtttggtg catatcaggg
    11341 tttgacacaa gaatttttcc atccaccata cctaaattat cgttttcatg gatttctaag
    11401 ccatcgatcg ttgtatcacg cacggctgtt gtgacgcttc cgctaacgac atcgctaaga
    11461 gcagctgtca tacgctcttg gttttcttca atggacttgc ttggatcaaa ggcaagaaga
    11521 cttgtcatac cttgaggaag agtgcgagcc tctaccacta ccgctggttg ctccaaaact
    11581 tctgccgcag attgagctgc catgaagatg ttcttgttgt ttggcaagaa gatgatgtta
    11641 cgggcattaa cctgttcaac agccttgata aagtcttctg ttgaagggtt catggtttga
    11701 ccgccttcga taacataatc cacgccttga gaacagaaga tatctgctag acctttacca
    11761 gccaccacag caatcaaagc atactctttt tcttcagccg acttgataac ttgagtagct
    11821 tctttctcaa cctgtgcttc gtgttggtta cgcatattgt caacttttac cttgaccaag
    11881 ctaccatatt tgagaccttc ttgcataaca agtcctggat cttctgtatg aacatggact
    11941 ttgacaattt catcatcgtt aacaacaagg agagaatctc caagctcatc caagtagtta
    12001 cggaattcat cgtagtcaaa atctttagca taggttggac cttgcttaag agctaccatg
    12061 atttcagtac agtaaccaaa cgtgatgtcc tcagtcgcta cgtgaccagc tacagactta
    12121 tgatgctcta cattgatcat ctcactcatg ttggcaggag tcgctacaaa gtcctcagat
    12181 gcaatatatt cgccagtaag ggctgaaagg aaaccttcgt agatgaagac caatccttga
    12241 ccacctgagt ccacaacgcc aacttctttc aatactggaa gcatgtctgg tgttttagct
    12301 agagctgttt tagcaccttc caaggctgcg cgcatgactt caacagcgtc atctgtttgc
    12361 tcagcttttt tcttagcacc gatagcagct ccacgagaaa ctgttaaaat cgttccttca
    12421 acaggtttca tcactgcctt ataggcaact tccacacctg attggaaggc cagagccaag
    12481 tcttgacctg ttaactcgtc tttatccttg atagcttggg aaaatccacg gaaaagctga
    12541 gacgtaatca ctcctgagtt cccacgcgca cccatcaaaa gccctttggc aagaatgctc
    12601 gctacttctc caactgtaga agctggcttg tctgcaactt ctttagcacc attttcaatg
    12661 gtcattccca tatttgtccc agtatctcca tctggaactg gaaagacgtt taatgaattg
    12721 acatattcag cttgcttatt caagcgagtt gatgcagcct gcaccatttc ttgaaataag
    12781 ctagtagtaa tttttgacac ggttattctc ctacaacttt gatattttga atgtagacat
    12841 ttacagtctg agcagtaatt ccaagctggt tttccaagct aaaggcaaca cgctcttgaa
    12901 tgttttttga cacttcacta atctttgttc cgtagcttaa cacggtatat acatcaactg
    12961 caatactgcc atcttcggct gcctttacga cgacaccttt agaataattt tccttaccta
    13021 gcagggcttg gaaattatct ttgagggcat ttttactagc cataccgacc acaccaaaaa
    13081 tctcagttgc tgctccacct acgacggttg caatcacttc atctgttagt tcgatttgac
    13141 catcttttgt attaattttt acagtcatcc tttttacctc aactagttga tactctattt
    13201 tatcatattt cagcccaagt gtaaaagcag aatgatgtat cagcggatat ttactctatt
    13261 tttcaaacga tgttataccc acaataaaag aaaaaagacc ctaaggtctc ctttgctttt
    13321 attattaaac gcgttcaact ttacctgatt tcaaagcacg agctgaagcc caaacttttt
    13381 taggtttacc atcgataaga acagtaactt tttgaaggtt tggttttacg gcacgttttg
    13441 tttggttcat cgcgtgtgaa cggttgtttc ctgatacagt cttacgacct gtaaagtaac
    13501 atactttagc cattgtgttt tcctcctatt agatctaata tagcggatgt gctagcacca
    13561 cataccgtac tatgttatca cattttcttg ttttttgcaa gggaattgga agatttttta
    13621 tttgtgtctt aaatcaggtc ttgcgtgaca ttttctgctc tccacatgcc atcgttgatt
    13681 aacagaacac cagaattaaa attatgtgta taaaaatcat ctctaactgc agctaagggt
    13741 atagccgtca agtccaaatc ccacagctca tctatcgatt ttcttacaac aatatctgaa
    13801 tccaaataca gtacacgaga ctcgcttaca tactttggaa taaaatacct aaaaaagccg
    13861 catatgaaag tccctcaaag gggagacgat aacctttcag aatattactg tcaatctaaa
    13921 cattcacaat ctcactattc aaagtctcta gtcttttttc catcaattgg aaccattctc
    13981 gcggaaggtc atcattaaaa acataaaact taagattata atgatgaaca caaagagatt
    14041 ttattgttgt ttcaacttta tccatataag cattatctgc acctaagaca atcgcttttt
    14101 tctcttcttt cactttttat ctcatttctt tttattccca tcatattatt cccatcatat
    14161 gtttcccatc atatgtttct acgtaaccat tattttcgcc tattcgttcg taaaaccata
    14221 ccagtggaga ttttagatga agtcccatta cggtttacaa tttttacatt acgacacgga
    14281 gttttacaaa tcgatttcat ttgccaaacg tagttagtga ggcagttagc tagttcgcca
    14341 aatagcgact agcgtccaac aatttggaac tttagttcca attgttggta ctgagtcaca
    14401 tcttctcctc taactctacg tctggatact tgtccgcaaa ccagcggagg gcaaagtcat
    14461 tttcaaagag aaagactggt tggtcaaaac ggtctttggc taagatattg cgacttgacg
    14521 acatccgttc atccaagtcc tcaggcttga tccaacgaac ggtcttttta cccattgggt
    14581 tcataactac ttccgcattg tactcgcctt ccatgcggtg tttaaagact tcaaactgga
    14641 gttgacctac agcgcctagc atgtactcac ctgtttggta attcttataa agctgaacgg
    14701 ctccttcttg caccaattgc tcaatcccct tgtggaagga tttttgcttc ataacattct
    14761 tagcagaaac tttcatgaaa atctcaggtg taaaggttgg caggggttca aattcaaact
    14821 tgttttttcc aaccgtcaag gtatccccaa cctgataagt accggtatcg taaaccccga
    14881 taatatcacc tgccacggca ttggtcacat tctcacgact ctccgccata aactgggtaa
    14941 cattagatag tttagccccc ttaccagtac gagggagatt gacactcatg ccgcgctcaa
    15001 attcgccaga tacgatacgg acaaaggcaa tacggtcacg gtgacgaggg tccatgttgg
    15061 cttggatttt aaagacaaag cctgagaaat ccttgtcata aggatccaca atttcaccgt
    15121 ctgttttctt gtgaccatgt ggttctggag caaacttgag gaaggtttca aggaaggtct
    15181 gcacaccaaa gtttgtcagg gctgaaccga aaaagacagg cgtcaattct ccagccagaa
    15241 tagcttcctc tgaaaactca ttcccggctt catttaaaag ctcaatgtca tccttgactt
    15301 gctcgtagaa aggattgcta ccaaagagtt tgtccccgtc ttctagactg gcaaaacgct
    15361 catccccttt gtaaagctct aaacgttggt tatagaggtc atacaagccc tcaaaggctt
    15421 tccccatccc gataggccag ttcatagggt agctagcaat gcccaagatt tcttccaatt
    15481 cttgcaagag atccaaaggc tcacgaccgt cacggtccag cttgttcata aaggtaaaga
    15541 ctggaatgcc acgatgtttc acaacctcaa acaatttctt ggtttgagcc tcgatcccct
    15601 tggcagagtc cacgaccatg accgcagcat ccaccgccat caaggtacga taggtatctt
    15661 ctgagaagtc ctcgtgccct ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa
    15721 attgcataac agatgaagta acagaaatcc cacgttgctt ctcgatatcc atccagtcag
    15781 atttagcaaa agtccctgtt ttcttccctt ttaccgtacc agcctcacga atctcacccc
    15841 caaagtagag taactgctca gtgatggttg ttttccccgc gtccgggtgg gagataatgg
    15901 caaaggtacg acgtttctta atttcttctt gaatattcat aagttctctt tctttgattc
    15961 tctatttttc ttgtttcaat agctgagaat gatttttaca ttggatttta ccattccttt
    16021 caacactcca ttatatcgga ttttagcatt tttttcaatt tctatttctt ttcacttccc
    16081 cctcccttat ttataggaaa atatggtaaa atagaacaga ctaaaaatca tcatttcacg
    16141 aaaggatgca agatgaaaat tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa
    16201 ttaagattct ctgaagaaga aactgctgcc tttgcgacca ccttgtctaa gattgttgac
    16261 atggttgaat tgctgggcga agttgacaca actggtgtcg cacctactac gactatggct
    16321 gaccgcaaga ctgtactccg ccctgatgtg gccgaagaag gaatagaccg tgatcgcttg
    16381 tttaaaaacg tacctgaaaa agacaactac tatatcaagg tgccagctat cctagacaat
    16441 ggaggagatg cctaatgact tttaacaata aaactattga agagttgcac aatctccttg
    16501 tctctaagga aatttctgca acagaattga cccaagcaac acttgaaaat atcaagtctc
    16561 gtgaggaagc cctcaattca tttgtcacca tcgctgagga gcaagctctt gttcaagcta
    16621 aagccattga tgaagctgga attgatgctg acaatgtcct ttcaggaatt ccacttgctg
    16681 ttaaggataa catctctaca gacggtattc tcacaactgc tgcctcaaaa atgctctaca
    16741 actatgagcc aatctttgat gcgacagctg ttgccaatgc aaaaaccaag ggcatgattg
    16801 tcgttggaaa gaccaacatg gacgaatttg ctatgggtgg ttcaggtgaa acttcacact
    16861 acggagcaac taaaaacgct tggaaccaca gcaaggttcc tggtgggtca tcaagtggtt
    16921 ctgccgcagc tgtagcctca ggacaagttc gcttgtcact tggttctgat actggtggtt
    16981 ccatccgcca acctgctgcc ttcaacggaa tcgttggtct caaaccaacc tacggaacag
    17041 tttcacgttt cggtctcatt gcctttggta gctcattaga ccagattgga ccttttgctc
    17101 ctactgttaa ggaaaatgcc ctcttgctca acgctattgc cagcgaagat gctaaagact
    17161 ctacttctgc tcctgtccgc atcgccgact ttacttcaaa aatcggccaa gacatcaagg
    17221 gtatgaaaat cgctttgcct aaggaatacc taggcgaagg aattgatcca gaggttaagg
    17281 aaacaatctt aaacgcggcc aaacactttg aaaaattggg tgctatcgtc gaagaagtca
    17341 gccttcctca ctctaaatac ggtgttgccg tttattacat catcgcttca tcagaagctt
    17401 catcaaactt gcaacgcttc gacggtatcc gttacggcta tcgcgcagaa gatgcaacca
    17461 accttgatga aatctatgta aacagccgaa gccaaggttt tggtgaagag gtaaaacgtc
    17521 gtatcatgct gggtactttc agtctttcat caggttacta tgatgcctac tacaaaaagg
    17581 ctggtcaagt ccgtaccctc atcattcaag atttcgaaaa agtcttcgcg gattacgatt
    17641 tgattttggg tccaactgct ccaagtgttg cctatgactt ggattctctc aaccatgacc
    17701 cagttgccat gtacttagcc gacctattga ccatacctgt aaacttggca ggactgcctg
    17761 gaatttcgat tcctgctgga ttctctcaag gtctacctgt cggactccaa ttgattggtc
    17821 ccaagtactc tgaggaaacc atttaccaag ctgctgctgc ttttgaagca acaacagact
    17881 accacaaaca acaacccgtg atttttggag gtgacaacta atgaactttg aaacagtcat
    17941 cggacttgaa gtccacgtag agctcaacac caattcaaaa atcttctcac ctacttctgc
    18001 ccactttgga aatgaccaaa atgccaacac taacgtgatt gactggtctt tcccaggagt
    18061 tctaccagtt ctcaataaag gggttgttga tgccggtatc aaggctgctc ttgccctcaa
    18121 catggacatc cacaaaaaga tgcactttga ccgcaagaac tacttctatc ctgataaccc
    18181 caaagcctac caaatttctc agtttgatga accaatcgga tataatggct ggattgaagt
    18241 caaactagaa gacggtacga ccaagaaaat cggtatcgaa cgtgcccacc tagaggaaga
    18301 cgctggtaaa aacacccatg gtacagatgg ctactcttat gttgacctca accgccaagg
    18361 ggttcccttg attgagattg tatctgaggc agatatgcgt tctcctgaag aagcctatgc
    18421 ttatctgaca gccctcaagg aagttatcca gtacgctggc atttctgacg ttaagatgga
    18481 ggaaggttcg atgcgtgtgg atgccaacat ctcccttcgt ccttatggtc aagagaaatt
    18541 cggtaccaag actgaattga agaacctcaa ctccttctca aacgttcgta aaggtcttga
    18601 atacgaagtc caacgccagg ctgaaattct tcgctcaggt ggtcaaatcc gccaagaaac
    18661 acgccgttac gatgaagcga ataaagcaac catcctcatg cgtgtcaagg aaggggctgc
    18721 tgactaccgc tacttcccag aaccagacct acccctcttt gaaatttctg acgagtggat
    18781 tgaggaaatg cggactgagt tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc
    18841 tgaccttggt ttatcagact acgatgctag tcagttgact gctaataaag tcacttctga
    18901 cttctttgaa aaagctgttg ccctaggtgg tgatgccaaa caagtctcta actggctcca
    18961 aggggaagtc gctcagttct tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac
    19021 accagaaaac ttggttgaaa tgattgccat catcgaagac ggtactattt catctaagat
    19081 tgccaagaaa gtctttgtcc atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga
    19141 aaaagcaggt atggttcaaa tttcagatcc agctatcttg atcccaatca tccaccaagt
    19201 ctttgccgat aacgaagctg ctgttgccga cttcaagtca ggcaaacgta acgccgacaa
    19261 ggcctttaca ggattcctta tgaaggcaac caaaggccaa gccaacccac aagttgccct
    19321 taaactactt gcacaggaat tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc
    19381 ctaaggttgg ttttttcttc tctaccaact cccaataact attttggctt tatttccaga
    19441 gtattttatg gtaaaatgaa gagtaataat atttattaaa gaggtaaaaa catgattgaa
    19501 gcaagtaaat taaaagctgg tatgaccttt gaaacagctg acggcaaatt gattcgcgtt
    19561 ttggaagcta gtcaccacaa accaggtaaa ggaaacacga tcatgcgtat gaaattgcgt
    19621 gatgtccgta ctggttctac atttgacaca agctaccgtc cagaggaaaa atttgaacaa
    19681 gctattatcg agactgtccc agctcaatac ttgtacaaaa tggatgacac agcatacttc
    19741 atgaatacag aaacttatga ccaatacgaa atccctgtag tcaatgttga aaacgaattg
    19801 ctttacatcc ttgaaaactc tgatgtgaaa atccaattct acggaactga agtgatcggt
    19861 gtcaccgttc ctactactgt tgagttgaca gttgctgaaa ctcaaccatc tatcaaaggt
    19921 gctactgtta caggttctgg taaaccagca acgatggaaa ctggacttgt cgtaaacgtt
    19981 ccagacttca tcgaagcagg acaaaaactc gttatcaaca ctgcagaagg aacttacgtt
    20041 tctcgtgcct aatctctaga aagaggtcat tctatgggaa ttgaagaaca acttggcgaa
    20101 atcgttatcg ccccacgtgt acttgaaaaa atcattgcta tcgctactgc aaaggtagag
    20161 ggtgttcact ctttttcaaa cagatcagtg tctgataccc tttcaaaact ttcactcggc
    20221 cgtggcattt atcttaaaaa cgtggacgaa gaactcacag cagatatcta tctctacctt
    20281 gagtacggag taaaagttcc taaggtagcg gttgctatcc agaaagctgt caaagatgcc
    20341 gtccgtaata tggctgatgt agaactcgct gctatcaata ttcacgttgc aggtatcgtc
    20401 ccagataaaa caccaaaacc agaattgaaa gatctatttg acgaggactt cctcaatgac
    20461 tagtccacta ttagaatcta gacgccaact ccgtaaatgc gcttttcaag ctctcatgag
    20521 ccttgagttc ggtacggatg tcgaaactgc ttgtcgtttc gcctatactc atgatcgtga
    20581 agatacggat gtacaacttc cagccttttt gatagacctc gtttctggtg ttcaagctaa
    20641 aaaggaagaa ctagataagc aaatcactca gcatttaaaa gcaggttgga ccattgaacg
    20701 cttaacgctc gtggagagaa acctccttcg cttgggagtc tttgaaatca cttcatttga
    20761 cactcctcag ctggttgctg ttaatgaagc tatcgagctt gcaaaggact tctccgatca
    20821 aaaatctgcc cgttttatca atggactgct cagccagttt gtaacagaag aacaataagg
    20881 ctctttgtca actgtagtgg gttgaaaaaa agctaagctc gagaaaggac aaatttcgtc
    20941 ctttcttttt tgatgttcaa agcgataaaa atccgttttt tgaagttttc aaagtttcga
    21001 aaaccaaagg cattgcgctt gataagtttg atgagattat tggtcgcttc cagtttggca
    21061 ttagaatagt gtagttgaag ggcgttgaca atcttttctt tatctttgag gaaggtttta
    21121 aagacagtct gaaaaatagg atgaacctgc ttaagattgt cctcaataag tccgaaaaat
    21181 ttctctggtt ccttattctg gaagtgaaac agcaagagct gatagagctg atagtggtgt
    21241 ttcaagtctt gtgaatggct caaaagcttg tctaaaatct ctttattggt taagtgcata
    21301 cgaaaagtag gacgataaaa tcgcttatca ctcagtctac ggctatcctg ttgaatgagt
    21361 ttccagtagc gcttgatatc cttgtattca tgggattttc gatgaaactg attcatgatt
    21421 tggacacgca cacgactcat ggcacggcta agatgttgta caatgtgaaa gcgatcaaga
    21481 acgattttag cattcgggag tgaaacagtc tgggagactg tttcagcctg agcctaggaa
    21541 tttgaaagcg aagctgttta gccaagtcat agtaagggct aaacatatcc atagtaataa
    21601 ttttgacgcg acatcggaca actctatcgt agcgaagaaa gtgatttcga atgatagctt
    21661 gtgttctacc ctcaagaaca gtgatgatat tgagattgtt aaaatcttgc gcaatgaagc
    21721 tcatctttcc ctttgtaaaa gcatactcat cccaagacat aatctcagga agacaagaaa
    21781 aatcatgttt aaagtgaaaa tcattgagct tacgaataac agttgaagtt gagatggaaa
    21841 gctgatgggc aatatcagtc atagaaatct tttcaatcaa cttttgagca atcttttggt
    21901 tgatgatacg agggatttgg tgatttttct tgacgaggga agtctcagcg accgctattt
    21961 ttgagcactg atagcactta aagcgacgct ttctaaggag gattctagta ggcataccag
    22021 ttgtctcaag gtaagg
//
LOCUS       DBDESG010000036        21864 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.036, whole genome shotgun sequence.
ACCESSION   DBDESG010000036 DBDESG010000000
VERSION     DBDESG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.036"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..514
                     /locus_tag="ACKB38_001323"
                     /pseudo
     CDS             1..514
                     /locus_tag="ACKB38_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(589..1509)
                     /gene="cysK"
                     /locus_tag="ACKB38_001324"
     CDS             complement(589..1509)
                     /gene="cysK"
                     /locus_tag="ACKB38_001324"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGL7980086.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1758..2393)
                     /locus_tag="ACKB38_001325"
     CDS             complement(1758..2393)
                     /locus_tag="ACKB38_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGL7980087.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2449..3747
                     /locus_tag="ACKB38_001326"
     CDS             2449..3747
                     /locus_tag="ACKB38_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGL7980088.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3744..4406
                     /locus_tag="ACKB38_001327"
     CDS             3744..4406
                     /locus_tag="ACKB38_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGL7980089.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4486..5034
                     /gene="raiA"
                     /locus_tag="ACKB38_001328"
     CDS             4486..5034
                     /gene="raiA"
                     /locus_tag="ACKB38_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGL7980090.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            5170..7143
                     /locus_tag="ACKB38_001329"
     CDS             5170..7143
                     /locus_tag="ACKB38_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGL7980091.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDMKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEMKEKEKEE"
     gene            7140..7592
                     /gene="rplI"
                     /locus_tag="ACKB38_001330"
     CDS             7140..7592
                     /gene="rplI"
                     /locus_tag="ACKB38_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGL7980092.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7636..8988
                     /gene="dnaB"
                     /locus_tag="ACKB38_001331"
     CDS             7636..8988
                     /gene="dnaB"
                     /locus_tag="ACKB38_001331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGL7980093.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8990..9256
                     /locus_tag="ACKB38_001332"
     CDS             8990..9256
                     /locus_tag="ACKB38_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGL7980094.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9345..10691
                     /gene="cbpD"
                     /locus_tag="ACKB38_001333"
     CDS             9345..10691
                     /gene="cbpD"
                     /locus_tag="ACKB38_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGL7980095.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10774..10876
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10947..11237
                     /locus_tag="ACKB38_001334"
     CDS             10947..11237
                     /locus_tag="ACKB38_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGL7980096.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11200..11958
                     /locus_tag="ACKB38_001335"
     CDS             11200..11958
                     /locus_tag="ACKB38_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980097.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            11997..13004
                     /locus_tag="ACKB38_001336"
     CDS             11997..13004
                     /locus_tag="ACKB38_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7980098.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            13004..13732
                     /locus_tag="ACKB38_001337"
     CDS             13004..13732
                     /locus_tag="ACKB38_001337"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980099.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13851..14309
                     /locus_tag="ACKB38_001338"
     CDS             13851..14309
                     /locus_tag="ACKB38_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGL7980100.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            14311..16743
                     /locus_tag="ACKB38_001339"
     CDS             14311..16743
                     /locus_tag="ACKB38_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGL7980101.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDSNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLTE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17385..18038
                     /gene="rr06"
                     /locus_tag="ACKB38_001340"
     CDS             17385..18038
                     /gene="rr06"
                     /locus_tag="ACKB38_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGL7980102.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            18035..19366
                     /gene="hk06"
                     /locus_tag="ACKB38_001341"
     CDS             18035..19366
                     /gene="hk06"
                     /locus_tag="ACKB38_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGL7980103.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19449..19976
                     /locus_tag="ACKB38_001342"
     CDS             19449..19976
                     /locus_tag="ACKB38_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGL7980104.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            20055..>21864
                     /gene="cbpA"
                     /locus_tag="ACKB38_001343"
     CDS             20055..>21864
                     /gene="cbpA"
                     /locus_tag="ACKB38_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HGL7980105.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLVMGSVVHATENEGAT
                     QVPTSSNRANESQAEQGEQPKKLDSERDKARKEVEEYVKKIVGESYAKSTKKRHTITV
                     ALVNELNNIKNEYLNKIVESTSESQLQILMMESRSKVDEAVSKFEKDSSSSSSSDSST
                     KPEASDTAKPNKPTEPGEKVAEAKKKVEEAEKKAKDQKEEDRRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKVKANEPRDEQKIKQAEAEVESKQAEATRLKKIKTDREEAEEEA
                     KRRADAKEQGKPKGRAKRGVPGELATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVA
                     EAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAESDVEVKKAELELVKEEAKEP
                     RNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPA
                     PAPKAEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPA
                     QPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYY
                     LNANGSMATGWLQNNGSWYYLNANGSMATGWLQYNGS"
BASE COUNT         6897 a         3645 c         4969 g         6353 t
ORIGIN      
        1 ctcctattcc taaagcccct ttagcgcata actttggctc agcttctatt atcgctcaca
       61 ccatccatca gaagtttaat ctgaaggtac ccaattatcg ccaagaagaa gattgggcta
      121 ggatgggttt accaatcaca cgtaaggaaa tctctaattg gcatatcaag acgagtcaat
      181 actatttgga gcccctttat aacctcttgc gagagagact attgactcag cccttacttc
      241 atgcggatga aacttcttat agggtgctag agagtgatag tcagctgact tactattgga
      301 cttttttgtc tgggaaagct gagaaacaag ggattacgct ttaccatcat gttctaatcg
      361 atttgttcat atcttatttc aatccattat aaatagtgag aaatatctat cctatcttct
      421 agaatgtctt ccaaacgtgg aaactctcgt aaacaaagag gttttagagg cctatttacc
      481 atggactaaa gttgtacaag aaaagtgcaa ataagaaatc tccagattag gaactatccg
      541 tgagttctct agtctggaga tttttcaata gacttcgtta ttggacggtt acaattcata
      601 aagtgctgta gagagataac gttcaccgtt atctggtgct agggcaagga cttttttacc
      661 tgtacctaat tttttggcaa cctcgatggc tccgtagata gctgcagctg aggaaatccc
      721 tacaaggaag ccttcttttc caccaatttc acgtccgagt gcaagagcgt catctgatgt
      781 tacacgaacg ataccatcat aggctttagt atcaagtgta tcaggaataa atccagctga
      841 gataccttga attttgtgag gaccaggttt ttcaccagat agaatagcag attcatctgc
      901 ttctactgca aaaacttgaa tgttagaatt ttctgatttg agtgcatgag aaacaccaga
      961 aatcgttcca ccagtaccta ctccagcaac aaaggcatct aatccatctt taccgaaagc
     1021 agctagtatc tcagctcctg ttgttctttc gtgtacttct ggattagctg gattgtcaaa
     1081 ttgaagagga aggaaaccat cacgttcagc agcgatttct tgagccttag caatagcacc
     1141 tttcattccc tcgctaccag gagttaggac gagttcagca ccataagctt ggataatttt
     1201 acgtcgttct acactcatag tttcaggcat aacgatgacg actttatacc ctttagcagc
     1261 acctacccat gaaagtccaa taccggtgtt tccacttgtt gcttcaacaa tagtagaacc
     1321 aggtttcaga ataccatctt gttcagcttt ttcaatcatg ctaagggcaa tacggtcttt
     1381 tacagatgaa ccaggattaa atgcttcaag ctttatatag acgtctgcag caccttctgg
     1441 cacgatgttg ttaagtttaa caatcggtgt ttgaccgatt aattcagtaa tgttgttata
     1501 aatagacata tgaatacctc tttgtataag atatctttag tataacgcta tccataccat
     1561 ttctatatat catagatcaa agtcattaat tttcaagtga ttgcgttcag ctaattctag
     1621 taaataggtg tgaaggaaaa cgatatcagg tttcgtttga attgcttcat tttctagtat
     1681 attttggtac gcctcttttt attttgctcg ctaaagtagt tgtgagaatg tcaacgaaac
     1741 gctgtattat tcattgttta cactaagttt acaggaactt caacctctcg taaaccttgg
     1801 tcagttaaag tgacttttcc attaaaaaac tccacaagtg cagctttaat agtttctttt
     1861 tcttctttat caacataaat catcgtatcg acttgatctg taaagtttgt atccagctcc
     1921 atgagaccat gttctttaag gaagttactg tactcttggt actgagcata agacatttga
     1981 atagcaatgc cagcctgttc ttttatttca ataataccaa tttctttgac agctaaggcg
     2041 acactgccgg cgtaagcacg aattagtcct ccagcgccta gtttaatacc accaaagtag
     2101 cgtgtcacga ccacacagac attggtgaga ttgtgatttt ctagtacccc aagcatggga
     2161 acaccagcag taccactagg ctcaccatca tcacttgtac gtttaatttc actacgttct
     2221 ccaataatga aggcagagca gttatgtgtc gctttgtagt gttctttttt gatggcagta
     2281 atgaagtcac gagcctcttc ttcgctataa acacgcttgg catggcagat aaagcgagat
     2341 tttttgattt cttcttggac ttgaccgtcc tctttaattg tcctaaattc catgatttaa
     2401 ttgtactaaa aaatcatcta aagtgctagt ttttacgaat aaagaagtat gaaagtaaat
     2461 ttagattatc tcggtcgttt atttactgag aatgaattaa cagaagaaga acgtcagttg
     2521 gcggagaaac ttccagcaat gagaaaggag aaggggaaac ttttctgtca acgctgtaat
     2581 agtactattc tagaagaatg gtatttgccc atcggtgctt actattgtcg agagtgcttg
     2641 ctgatgaagc gagtcagaag tgatcaaact ttatactatt ttccgcagga ggattttcca
     2701 aagcaagatg ttctcaaatg gcgcggccaa ttaactcctt ttcaagagaa ggtgtcagag
     2761 ggattgcttc aagtagtaga caagcaaaag ccaaccttag ttcatgcggt aacaggagct
     2821 ggaaagacag aaatgattta tcaagtagtg gctaaagtga tcaatgcggg tggtgcagtg
     2881 tgtttggcta gtcctcgcat agatgtttgt ttggagctgt acaagcgcct gcaacaggat
     2941 ttttcttgcg ggatagcttt gctacatgga gaatcggaac cttattttcg aacaccacta
     3001 gttgttgcaa caacccatca gttattgaag ttttatcaag cttttgattt gctgatagtg
     3061 gatgaagtag atgcttttcc ttatgttgat aatcccatgc tttaccacgc tgtcaagaat
     3121 agtgtaaagg agaatggatt gagaatcttt ttaacagcga cttcgaccaa tgagttagat
     3181 aaaaaggtcc gtttaggaga actaaaaaga ctgaatttac cgagacggtt tcatggaaat
     3241 ccgttgatta ttccaaaacc aatttggtta tcggatttta atcgctactt agacaagaat
     3301 cgtttgtcac caaagttaaa gtcctatatt gagaagcaga gaaagacagc ttatccgtta
     3361 ctcatttttg cttcagaaat taagaaaggg gagcagttag cagaaatctt acaggagcaa
     3421 tttccaaatg agaaaattgg ctttgtatct tctgtaacag aggatcgatt agagcaagta
     3481 caagcttttc gagatggaga actgacaata cttatcagta cgacaatctt ggagcgcgga
     3541 gttaccttcc cttgtgtgga tgttttcgta gtagaggcca atcatcgttt gtttaccaag
     3601 tctagtttga ttcagattgg tggacgagtt ggacgaagca tggatagacc gacaggagat
     3661 ttgcttttct tccatgatgg gttaaatgct tcaatcaaga aggcgattaa ggaaattcag
     3721 atgatgaata aggaggctgg tctatgaagt gcttgttatg tgggcagact atgaagactg
     3781 ttttaacttt tagtagtctc ttacttctga ggaatgatga ctcttgtctt tgttcagact
     3841 gtgattctac ttttgaaaga attggggaag agaactgtcc aaattgtatg aaaacagagt
     3901 tgtcaacaaa gtgtcaagat tgtcaacttt ggtgtaaaga gggagttgaa gtcagtcata
     3961 gagcgatttt tacttacaat caagctatga aggatttttt cagtcggtat aagtttgatg
     4021 gagacttcct gttaagaaaa gttttcgctt catttttaag tgaggagttg aaaaagtaca
     4081 aagagtatca atttgttgta attcccctaa gtcctgatag atatgctaat agaggattta
     4141 atcaggttga gggcttggta gaggcagcag gctttgagta tctggattta ttagagaaaa
     4201 gagaagagag agccagttct tctaaaaatc gttcagagcg cttggggaca gaacttcctt
     4261 tctttattaa aagtggagtc actattccta aaaaaatcct acttatagat gatatctata
     4321 ctacaggagc aactataaat cgtgttaaga aactgttgga agaagctggt gctaaggatg
     4381 taaaaacatt ttcccttgta agatgaggaa aaaatttgca aaaatagaat gaaagcgtta
     4441 taataaaatc ataaaaacaa aaatgtttag aaagaaggta ctcatatgat taaatatagt
     4501 atccgtggtg aaaacctaga agtaacagaa gcaattcgtg attatgtagt ttctaaactc
     4561 gaaaagatcg aaaagtactt ccaaccagaa caagagttgg atgcccgaat taacttaaaa
     4621 gtttatcgtg aaaaaacggc taaagtggaa gtaacgattc cgcttggatc tattactctc
     4681 cgcgcagaag atgtatctca agatatgtat ggttcaattg accttgtaac tgataaaatt
     4741 gaacgtcaga ttcgtaaaaa taaaacaaaa atcgagcgta aaaataaaaa taaggtagca
     4801 actggtcaat tatttacaga tgctttggtg gaagattcaa atattgtcca gtctaaagtt
     4861 gttcgttcaa aacaaattga tttaaaacca atggatttgg aagaagcaat tctacaaatg
     4921 gatttattgg ggcatgattt ctttatctat gtggatgttg aagatcagac aaccaatgtg
     4981 atttatcgtc gtgaggatgg cgaaattggt ttgttagagg ttaaagaatc ttaatttcaa
     5041 tatgtgtaaa ggtaggttta ctgaattgta aactaccttt ttcttataat tgatagaatt
     5101 actgattaca cttttaaaaa gtcgcttttt gaggggtatt atggtataat gggtgccaag
     5161 aggttttgaa tgaaaaaatt ttatgtaagt ccaatttttc ctattctagt aggattgatt
     5221 gcgtttggag tcttatccac tttcattatt tttgttaata ataatctgtt gacggtttta
     5281 attttgtttc tttttgtagg aggctatgtt tttttattta agaaactgag agtgcattat
     5341 acaaggagtg atgtagaaca gatacagtat gtaaaccacc aagcggaaga aagtttgaca
     5401 gctctattgg aacagatgcc tgtaggtgtt atgaaattga atttatcttc tggagaggtt
     5461 gagtggttta atccctatgc tgaattgatt ttgaccaagg aagatggtga ttttgattta
     5521 gaagctgttc aaacgattat caaggcttca gtaggaaatc cgtctactta tgccaagctt
     5581 ggtgagaagc gttatgctgt tcatatggat gcttcttccg gtgttttgta ttttgtagat
     5641 gtatccaggg aacaagccat aacagatgaa ttggtaacaa gtagaccagt gattgggatt
     5701 gtctctgtgg ataattatga tgatttggag gatgaaactt ctgagtcaga tattagtcaa
     5761 atcaatagtt ttgtagctaa ttttatatca gagttttcag aaaaacacat gatgttttct
     5821 cgtcgggtaa gtatggatcg attttatcta tttactgact acacggtgct tgagggcttg
     5881 atgaatgata aattttctgt tattgatgct ttcagagaag agtcgaaaca gagacagttg
     5941 cccttgacct taagtatggg attttcttat ggcgatggaa atcatgatga gatagggaaa
     6001 gttgctttgc tcaatttgaa cttggctgaa gtacgtggtg gcgaccaggt ggttgttaag
     6061 gaaaacgacg aaacgaaaaa tccagtttat tttggtggtg ggtctgctgc ttcaatcaag
     6121 cgtacacgga ctcgtacgcg cgctatgatg acagctattt cagataagat tcggagtgta
     6181 gatcaggttt ttgtagtcgg tcacaaaaat ttagacatgg atgctttggg ctctgctgta
     6241 ggtatgcagt tgttcgccag caatgtgatt gaaaatagct atgctcttta tgatgaagaa
     6301 caaatgtctc cagatattga acgagctgtt tcattcatag aaaaagaagg agttacgaag
     6361 ttgttgtctg ttaaggatgc aatggggatg gtgaccaatc gttctttgtt gattcttgta
     6421 gaccattcaa agacagcctt aacattatca aaagaatttt atgatttatt tacccaaacc
     6481 attgttattg accaccatag aagggatcag gattttccag ataatgcggt tattacttat
     6541 atcgaaagtg gtgcaagtag tgccagtgag ttggtaacgg aattgattca gttccagaat
     6601 tctaagaaaa atcgtttgag tcgtatgcaa gcaagtgtct tgatggctgg tatgatgttg
     6661 gatactaaaa atttcacctc gcgagtaact agtcggacat ttgatgttgc tagctatctc
     6721 agaacgcgcg gaagtgatag tattgctatc caggaaatcg ctgcgacaga ttttgaagaa
     6781 tatcgtgagg tcaatgaact tattttacag gggcgtaaat taggttcaga tgtactaata
     6841 gcagaggcta aggacatgaa atgctatgat acagttgtta ttagtaaggc agcagatgcc
     6901 atgttagcca tgtcaggtat tgaagcgagt tttgttcttg cgaagaatac acaaggattt
     6961 atctctatct cagctcgaag tcgtagtaaa ctgaatgtac aacggattat ggaagagtta
     7021 ggcggtggag gccactttaa tttggcagca gctcaaatta aagatgtaac cttgtcagaa
     7081 gcaggtgaaa aactgacaga aattgtatta aatgaaatga aggaaaagga gaaagaagaa
     7141 tgaaagtaat ctttttagca gatgttaaag gaaaaggtaa aaaaggcgaa attaaggaag
     7201 taccaacagg gtatgcgcaa aactttctta tcaaaaagaa tctagccaaa gaagcgactg
     7261 ctcaagctgt aggtgaactt cgtggtaaac aaaaatcgga agaaaaagct cacgctgaga
     7321 tgattgcaga aggaaaagca attaaagcac aacttgaagc agaagaaact gttgtagaat
     7381 ttgttgaaaa agttggtcca gatggtcgta cctttggttc tattaccaat aagaagattg
     7441 cagaagaatt gcaaaagcaa tttggaatta agattgataa acgtcatatt caagtacaag
     7501 ctccgattcg agcggttggt ttgattgatg tgccagtgaa aatctatcaa gatatcacaa
     7561 gtgtaatcaa tcttcgtgtg aaagaaggat aagtttacac cttcttgaca agattgtaaa
     7621 aggaagggaa gtctgatggc agaagtagaa gagttacgag tacaacctca agatatctta
     7681 gctgagcaat ccgttttagg ggctatcttt attgatgaga gtaaacttgt ttttgtgcga
     7741 gaatacattg agtctcggga cttttttaag tatgcccatc gtttgatttt ccaagccatg
     7801 gtcgatttat ccgatcgtgg tgatgccata gatgcaacaa cggttcgtac tatccttgat
     7861 aatcaaggtg atttacagaa tattggtggc ttgtcttact tggttgagat tgttaattct
     7921 gtgccaactt ctgctaatgc ggagtattat gctaagattg ttgcagaaaa agcaatgcta
     7981 cgtcgtttaa ttgccaagtt gacagagtct gtcaaccaag cttacgaagc gtcacaacca
     8041 gctgatgaaa ttattgctca ggcagaaaaa gggttgattg atgtcagtga aaatgcaaat
     8101 cgaagcgggt ttaagaacat tcgagatgtg ttgaatctca actttggaaa tctggaagct
     8161 cgctcgcaac aaacgaccga tattacaggt attgcgacag gttatcgtga tttggatcat
     8221 atgacaacag gacttcatga ggaggagttg attatcttag cagctcgtcc agcagttggt
     8281 aagacagcat ttgccttgaa tatcgctcag aacattggga ctaagttgga caaaacggtt
     8341 gctatttttt cactcgaaat gggtgcggaa agcttggtag atcgtatgtt agctgcagaa
     8401 ggcttagtgg agtcacattc tatccgtaca gggcaattga cagatgagga gtggcaaaaa
     8461 tatactattg ctcagggtaa tctagctaac gccagtatct atatcgatga tacgccaggt
     8521 attcggatta cagagattcg ttctcgttct cgtaaattgg ctcaagaaac tggaaatctt
     8581 ggtttgattt tgatagacta tttgcaactt atcacgggaa ctggtcgaga aaatcgtcaa
     8641 caagaagttt ctgaaatttc tcgtcagttg aaaatactag ccaaggaatt gaaggttcca
     8701 gtaatcgctc tgagtcagct ttctcgtggt gtagaacaac gtcaggacaa gagaccggtc
     8761 ttgtctgata ttcgtgaatc tgggtctatt gagcaggacg ctgatatcgt agcttttctc
     8821 tatcgcgatg actactatga acgtggtggt gaagaagagg agggtatccc aaataataag
     8881 gtggaagtta ttatcgagaa aaaccgtagt ggagctcgtg gaacagtgga attgattttc
     8941 caaaaagaat acaataaatt ttcaagtatc tcaaagaggg aggcataaga tgtcagatgc
     9001 atttacagat gtagccaaga tgaaaaaaat caaagaagaa atcaaggcac atgagggaca
     9061 agtcgtagaa atgactttgg agaatggtcg taagcgccaa aaaaatagat tgggtaagct
     9121 aattgaagtt tatccatctc tatttattgt ggagtttggg gatgtggaag gagataaaca
     9181 agttaatgtt tacgttgaat cctttactta ctcagatatt cttacagaaa agaatttgat
     9241 tcattatctt gactaaagtg agaaattttc tcactttttc ttttttctcc gaataattta
     9301 ggtgaaggca atcatcgctt tatattattt ttcaaggagg aagaatgaaa attttaccgt
     9361 ttatagcaag aggaacaagt tattacttga agatgtcagt taaaaagctt gttccttttt
     9421 tagtagtagg attgatgcta gcagctggtg atagtgtcta tgcctattcc agaggaaatg
     9481 gatcgattgc gcgtggggat gattatcctg cttattataa aaatgggagc caggagattg
     9541 atcagtggcg catgtattct cgtcagtgta cttcttttgt agcctttcgt ttgagtaatg
     9601 tcaatggttt tgaaattccg gcagcttatg gaaatgcgaa tgaatgggga catcgtgctc
     9661 gtcgggaagg ttatcgtgta gataatacac cgacgattgg ttccattact tggtctactg
     9721 caggaactta tggtcatgtt gcctgggtgt caaatgtaat gggagatcag attgagattg
     9781 aggaatataa ctatggttat acagaatcct ataataaacg agttataaaa gcaaacacga
     9841 tgacaggatt tattcatttt aaagatttgg atggtggcag tgttgggaat agtcaatcct
     9901 caacttcaac aggcggaact cattatttta agaccaagtc tgctattaaa actgaacctc
     9961 tagctagcgg aactgtgatt gattactatt atcctgggga gaaggttcat tatgatcaga
    10021 tacttgaaaa agacggctat aagtggttga gttatactgc ctataatgga agctatcgtt
    10081 atgttcaatt ggaggctgtg aataaaaatc ctctaggtaa ttctgttctt tcttcaacag
    10141 gtggaactca ttattttaag accaagtctg ctatcaaaac tgaaccccta gttagtgcaa
    10201 ctgtgattga ttactattat cctggagaga aggttcatta tgatcaaatt ctcgaaaaag
    10261 acggctacaa gtggttgagt tatacggctt ataacggaag tcgtcgctat atacagctag
    10321 agggagtgac ttcttcacaa aattatcaga atcaatcagg aaacatctct agctatggat
    10381 cccatagtag ttcaactgtc ggttggaaga aaataaatgg tagttggtat catttcaaat
    10441 caaatggttc taaatcaaca ggatggctga aagacggttc tagctggtat tatttgaaat
    10501 tatctggtga aatgcagaca ggatggttaa aggaaaatgg tttgtggtat tatctgggta
    10561 gttcaggggc aatgaaaaca ggctggtacc aggtctctgg taagtggtat tattcttact
    10621 cttcaggcgc cttagctgtt aatacgacgg tggatggcta cagagtaaac agtgatggag
    10681 aacgagtata gaaaattgga gtaggagaaa tttcctgctc ttttctgtaa gcagtttcct
    10741 tgacattttt cttattttgc gctatcatat atacatataa tatatgggag tctgtgtaca
    10801 gtctgagagg aagtgttaaa cttcgaccgc acctgatctg ggtaatgcca gcggagggaa
    10861 cgatacttag tctaattttg caccttttcc atgtatggta aaggtttttc tttttttaaa
    10921 aaggaaaacg agaagaggag gttcttatga aagcaagcat tgccttgcaa gttttacccc
    10981 tagtacaggg gattgatcgg atagctgtta ttgatcaggt cattgcttat ctgcaaactc
    11041 aagaagtgac gatggtagtg acaccatttg aaacggtctt ggaaggggag tttgatgagc
    11101 ttatgcgcat tctaaaagaa gcgctggaag tggcagggca ggaggcagac aatgtctttg
    11161 ccaatgtcaa aataaatgta ggagagattt taagtattga tgagaaactt gagaagtata
    11221 ctgagacgac acattagtct attgggcttt ctcggagtat tgtcaatctg gcagttagca
    11281 ggttttctta aacttctccc caagtttatc ctgccgacac ctcttgaaat tctccagccc
    11341 tttgttcgtg acagagaatt tctctggcac catagctggg cgaccttgag agtggcttta
    11401 ctggggctga ttttgggagt tttgattgcc tgtcttatgg ctgtgctcat ggatagtttg
    11461 acttggctca atgacctgat ttaccctatg atggtggtca ttcagaccat tccgaccatt
    11521 gccatagctc ctatcctggt cttgtggcta ggttatggga ttttgcccaa gattgtcttg
    11581 attatcttaa cgacaacctt tcccatcatc gttagtattt tggacggttt taggcattgc
    11641 gacaaggata tgctgacctt gtttagtctg atgcgggcca agccttggca aatcctgtgg
    11701 cattttaaaa tcccagttag cctgccttac ttttatgcag gtctgagggt cagtgtctcc
    11761 tacgccttta tcacaactgt ggtatctgag tggttgggag gttttgaagg tcttggtgtt
    11821 tatatgattc agtctaaaaa actgtttcag tatgatacca tgtttgccat tattattctg
    11881 gtgtcgatta tcagtctttt gggtatgaag ctggtcgata tcagtgaaaa atatgtgatt
    11941 aaatggaaac gttcgtagaa ttagaatgtt tctgaaaaag aaaagaggaa atcaaaatga
    12001 agaaaacatg gaaagtgttt ttaacgcttg taacagctct tgtagctgtt gtgcttgtgg
    12061 cctgtggtca aggaactgct tctaaagaca acaaagaggc agaacttaag aaggttgact
    12121 ttatcctaga ctggacacca aataccaacc acacagggct ttatgttgcc aaggaaaaag
    12181 gttatttcaa agaagctgga gtggatgttg atttgaaatt gccaccagaa gaaagttctt
    12241 ctgacttggt tatcaacgga aaggcaccat ttgcagtgta tttccaagac tacatggcta
    12301 agaaattgga aaaaggagca ggaatcactg ccgttgcagc tattgttgaa cacaatacat
    12361 caggaatcat ctctcgtaaa tctgataatg taagcagtcc aaaagacttg gttggtaaga
    12421 aatatgggac atggaatgac ccaactgaac ttgctatgtt gaaaaccttg gtagaatctc
    12481 aaggtggaga ctttgagaag gttgaaaaag taccaaataa cgactcaaac tcaatcacac
    12541 cgattgccaa tggcgtcttt gatactgctt ggatttacta cggttgggat ggtatccttg
    12601 ctaaatctca aggtgtagat gctaacttca tgtacttgaa agactatgtc aaggagtttg
    12661 actactattc accagttatc atcgcaaaca acgactatct gaaagataac aaagaagaag
    12721 ctcgcaaagt catccaagcc atcaaaaaag gctaccaata tgccatggaa catccagaag
    12781 aagctgcaga tattctcatc aagaatgcac ctgaactcaa ggaaaaacgt gactttgtca
    12841 tcgaatctca aaaatacttg tcaaaagaat acgcaagcga caaggaaaaa tggggtcaat
    12901 ttgacgcagc tcgctggaat gctttctaca aatgggataa agaaaatggt atccttaaag
    12961 aagacttgac agacaaaggc ttcaccaacg aatttgtgaa ataatgacag aaattagact
    13021 agagcacgtc agttatgcct atggtcagga gaggatttta gaggatatca acctacaggt
    13081 gacttcaggc gaagtggttt ccatcctagg cccaagtggt gttggaaaga ccaccctctt
    13141 taatctaatc gctgggattt tagaagttca gtcagggaga attgtccttg atggtgaaga
    13201 aaatcccaag gggcgcgtga gttatatgtt gcaaaaggat ctgctcttgg agcacaagac
    13261 ggtgcttgga aatatcattc tgcccctctt gattcaaaag gtggataagg cagaagctat
    13321 ttcccgagcg gataaaattc ttgcgacctt ccagctgaca gctgtaagag acaagtatcc
    13381 tcatgaactt agcggtggga tgcgccagcg tgtagcctta ctccggacct acctttttgg
    13441 gcacaagctc tttctcttag atgaggcctt tagcgccttg gatgagatga caaagatgga
    13501 actccacgct tggtatcttg agattcacaa gcagttgcag ctaacaaccc tgatcatcac
    13561 gcatagtatt gaggaggccc tcaatctcag cgaccgtatc tatatcttga aaaatcgccc
    13621 tgggcagatt gtttcagaaa ttaaactaga ttggtctgaa gatgaggaca aggaagtcca
    13681 aaagattgcc tacaaacgtc aaattttggc ggaattaggc ttagataagt agaaaaatag
    13741 ggagttggtg aagattatcc tttaccagcg ccctttttct tttaaaaatg agaaaatttc
    13801 ggtataatag tcaaacaagg tcaaggttta aagagagagg tgggtttgtt atgagattta
    13861 aaaatacatc ggatcatatt gaggcctaca tcaaggcgat tttagatcaa tctggtatcg
    13921 tggagttgca acggagtcag ttggcagata cctttcaggt tgttcctagt cagattaact
    13981 acgtgatcaa gacacgcttt acggaaagta gaggctactt ggttgaaagt aagcgtggtg
    14041 gcggaggcta cattcgtata ggacggattg agttttctag tcatcatgaa atgctccggg
    14101 agctgcttta ctcgattggt gagcgagtca gtcaagaaat ttatgaggat attctccagc
    14161 ttttggttga gcaggaattg atgaccaagc aggagatgaa tttgctagaa tcagtagctt
    14221 tggatcgcgt tttaggagaa gaagctccag ttgttcgagc aaacatgcta cgtcagatca
    14281 tacaagaggt agatagaaaa gggaagtaag atgaactatt caaaagcatt gaatgaatgt
    14341 atcgaaagtg cctacatggt tgctggacat tttggagctc gttatctaga gtcgtggcac
    14401 ttgttgattg ccatgtctaa tcacagttat agtgtagcag gggcaacttt aaatgattat
    14461 ccgtatgaga tggaccgttt agaagaggtg gctttggaac tgactgaaac ggactatagc
    14521 caggatgaaa cctttacgga attgccgttc tcccgtcgtt tgcaggttct ttttgatgaa
    14581 gcagagtatg tagcgtcagt ggtccatgct aaggtactag ggacagagca cgtcctctat
    14641 gcgattttgc atgatagcaa tgccttggcg actcgtatct tggagagggc tggtttttct
    14701 tatgaagaca agaaagatca ggtcaagatt gctgctcttc gtcgaaattt agaagaacgg
    14761 gcaggctgga ctcgtgaaga tctcaaggct ttacgccaac gccatcgtac agtagctgac
    14821 aagcaaaatt ctatggccaa tatgatgggc atgccgcaga ctcctagtgg tggtctcgag
    14881 gattatacgc atgatttgac agagcaagcg cgttctggca agttagaacc agtcatcggt
    14941 cgggacaagg aaatctcacg tatgattcaa atcttgagcc ggaagactaa gaacaaccct
    15001 gtcttggttg gggatgctgg tgtcgggaaa acagctctgg cgcttggtct tgcccagcgt
    15061 attgctagtg gtgacgtgcc tgcggaaatg gctaagatgc gcgtgttaga acttgatttg
    15121 atgaatgtcg ttgcagggac acgcttccgt ggtgactttg aagaacgcat gaataatatc
    15181 atcaaggata ttgaagaaga tggccaagtc atcctcttta tcgatgaact ccacaccatc
    15241 atgggttctg gtagcgggat tgattcgact ctggatgcgg ccaatatctt gaaaccagcc
    15301 ttggcgcgtg gaactttgag aacggttggt gccactactc aggaagaata tcaaaaacat
    15361 atcgaaaaag atgcggcact ttctcgtcgt ttcgctaaag tgacgattga agaaccaagt
    15421 gtggcagata gtatgactat tttacaaggt ttgaaggcga cttatgagaa acatcaccgt
    15481 gtacaaatca cagatgaagc ggttgaaaca gcggttaaga tggctcatcg ttatttaacc
    15541 agtcgtcact tgccagactc tgctatcgat ctcttggatg aggcggcagc aacagtgcaa
    15601 aataaggcaa agcatgtaaa agcagacgat tcagatttga gtccagctga caaggccctg
    15661 atggatggca agtggaaaca ggcagcccag ctaatcgcaa aagaagagga agtacctgtc
    15721 tacaaagact tggtgacaga gtctgatatt ttgaccacct tgagtcgctt gtcaggaatc
    15781 ccagttcaaa aactgactca aacggatgct aagaagtatt taaatcttga agcagaactc
    15841 cataaacggg ttatcggtca agatcaagct gtttcaagca ttagccgtgc cattcgccgc
    15901 aaccagtcag ggattcgcag tcataagcgt ccgattggtt cctttatgtt cctagggcct
    15961 acaggtgtcg ggaaaactga attagccaag gctctggcag aagttctttt tgacgacgaa
    16021 tcagccctta tccgctttga tatgagtgag tatatggaga aatttgcagc tagtcgtctc
    16081 aacggagctc ctccaggcta tgtaggatat gaagaaggtg gggagttgac agagaaggtt
    16141 cgcaataaac cctattccgt tctcctcttt gatgaggtag agaaggccca cccagatatc
    16201 tttaatgttc tcttgcaggt tctggatgac ggtgtcttga cagatagcaa gggacgcaag
    16261 gtcgattttt caaataccat tatcattatg acatcgaatc taggtgcgac tgcccttcgt
    16321 gatgataaga ctgttggttt tggggctaag gatattcgtt ttgaccagga aaatatggaa
    16381 aaacgcatgt ttgaagaact gaaaaaagct tatagaccgg aattcatcaa ccgtattgat
    16441 gagaaggtgg tcttccatag cctatctagt gatcatatgc aggaagtggt gaagattatg
    16501 gtcaagcctt tagtggcaag tttgactgaa aaaggcattg acttgaaatt acaagcttca
    16561 gctctgaaat tgttagcaaa tcaaggatat gacccagaga tgggagctcg cccacttcgc
    16621 agaaccctgc aaacagaagt ggaggacaag ttggcagaac ttcttctcaa gggagattta
    16681 gtggcaggca gcacacttaa gattggtgtc aaagcaggcc agttaaaatt tgatattgca
    16741 taaaagaata aaagtatcag catctgacca taagtcacag tggagtgaaa ttcaatgaaa
    16801 atcaaagagc aaactaggca gctagccgca ggttgctcaa aacactggtt tgaggttgca
    16861 gatagagctg acgtggtttg aagagatttt cgaagagtat gaaactaaaa cctatagctt
    16921 ctaaacgatc cgtggttttc atcattcaac acaaaattca tatgtttatt accctccgtc
    16981 gtatttgtct tagagcgtgt gtagtagaaa aagagcagtc ttatctgaaa tttttattct
    17041 ttcaaaagag acctgtttct tttttgcatg tcaaatccgt tctagctggt atttgaaaaa
    17101 tcaaactaat attcaatgaa aatcaaagaa caaactagga agctagccgc aggttgctca
    17161 aaacactgtt ttgaggttgt agatagagct gacgtggttt gaagagattt tcgaagagta
    17221 taagctgcaa gatgaatgat tttcttgtat tgacgttgtt gttgacaaaa agtagcggat
    17281 aaatgaaatc cattccatta tcatagatga taggctggta ggaaattttc aaatagcata
    17341 caggaaatag atgtatggag ttctggtagt agaaagggag agagatgaac attttagttg
    17401 cagatgacga ggaaatgatt agagaaggaa ttgcagcatt tctgacagaa gagggttatc
    17461 atgtcattat ggctaaggat ggacaagagg tcttggaaaa atttcaagat ctccctatcc
    17521 atctcatggt actggattta atgatgccta ggaagagtgg ttttgaagtg ttaaaagaaa
    17581 tcaatcaaaa gcacgatatt cctgtcatcg tcttgagtgc tctgggagat gaaactactc
    17641 agtcacaggt atttgatctc tatgctgatg atcatgtgac aaaacctttt tctttggtac
    17701 tgcttgtcaa gcgtattaag gcgcttatca gacgttacta cgtcatagag gatctttggc
    17761 gatatcagga tgtaacagtg gattttacct cttacaaagc acattataaa aatgaagaaa
    17821 ttgatctcaa accaaaggaa ttactggtac taaagtgttt gattcagcat aaaaatcaag
    17881 ttttaagtag agagcagata ttggaagaaa tttcaaaaga tgtagctgat ttaccttgtg
    17941 atagggtcgt tgatgtctat attcgtactc ttcgcaaaaa attagcttta gattgtatcg
    18001 tgactgtgaa aaatgttggg tataagatta gcttatgata aaaaatccta aattattaac
    18061 caagtctttt ttaagaagtt ttgcaattct aggtggtgtt ggtctagtca ttcatatagc
    18121 tatttatttg acctttcctt tttattatat tcaactggag ggggaaaagt ttaatgagag
    18181 cgcaagagtg tttacggagt atttaaagac taagacatct gatgaaattc caagcttact
    18241 ccagtcttat tcaaagtcct tgaccatatc tgctcacctt aaaagagata ttgtagataa
    18301 gcggctccct cttgtgcatg acttggatat taaagatgga aagctatcaa attatatcgt
    18361 gatgttagat atgtctgtta gtacagcaga tggtaaacag gtaaccgtgc aatttgttca
    18421 cggggtggat gtctacaaag aagcaaagaa tattttgctt ttgtatctcc catatacatt
    18481 tttggttaca attgcttttt cctttgtttt ttcttatttt tatactaaac gcttgctcaa
    18541 tcctcttttt tacatttcag aagtgactag taaaatgcaa gatttggatg acaatattcg
    18601 ttttgatgaa agtaggaaag atgaagttgg tgaagttgga aaacagatta atggtatgta
    18661 tgagcacttg ttgaaggtta tttatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt
    18721 gcaaaatcaa aaggtttcct ttgtccgcgg agcatcacat gagttgaaaa cccctttagc
    18781 cagtcttaga attatcctag agaatatgca gcataatatt ggagattaca aagatcatcc
    18841 aaaatatatt gcaaagagta taaataagat tgaccagatg agccacttat tagaagaagt
    18901 actggagtct tctaaattcc aagagtggac agagtgtcgt gagaccttga ctgttaagcc
    18961 agttttagta gatattttat cacgttatca agaattagct cattcaatag gtgttacaat
    19021 tgaaaatcaa ttgacagatg ctaccagggt cgtcatgagt cttagggcat tggataaggt
    19081 tttgacaaac ctgattagta atgcaattaa atattcagat aaaaatgggc gtgtaatcat
    19141 atccgagcaa gatggctatc tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga
    19201 actagaacat ttatttgata tattctatca ttctcaaatc gtgacagata aggatgaaag
    19261 ttccggtttg ggtctttaca ttgtgaataa tattttagaa agctatcaaa tggattatag
    19321 ttttctccct tatgaacacg gtatggaatt taagattagc ttgtagacag attagttttt
    19381 tattaaagtt catatagggt taacataagt gtgttattct ttgtgtagat aaaagaaagg
    19441 atactaatat ggtattagcg attattttag taacattctt tattcgattg atttttttaa
    19501 agcgttcgat agagaatgag aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg
    19561 agaatacaaa acgattaact atggctcata ttgtttttta tctctcttgc ttggttgagg
    19621 caatggtgca caagacaatt tttgatggca tgggcatggt tggtttagtc ttgcttattt
    19681 tttctatgct gatgttgatg ttggtgattc acttgttggg agatatttgg acagtgaagc
    19741 ttatgcttgt caataatcac aaatatgtag atcatatctt gtttaggaca gtaaaacacc
    19801 ctaattactt tttaaatatt cttcctgagt tgattggctt gaccttgttg agtcatgctt
    19861 atgtgacttt tgttttagtt tttccagttt atgcagttat tttgtatcga cgaatagctg
    19921 aagaggaaaa gctattacat gaagttataa tcccaaatgg aagcataaag agataaatac
    19981 aaaattcgat ttatatacag ttcatattga agtgatatag taaggttaaa gaaaaaatat
    20041 agaaggaaat aaacatgttt gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta
    20101 aatttagtgt tggagtagct agtgtagttg ttgccagtct tgttatggga agtgtggttc
    20161 atgcgacaga gaacgaggga gctacccaag tacccacttc ttctaatagg gcaaatgaaa
    20221 gtcaggcaga acaaggagaa caacctaaaa aactcgattc agaacgagat aaggcaagga
    20281 aagaggtcga ggaatatgta aaaaaaatag tgggtgagag ctatgcaaaa tcaactaaaa
    20341 agcgacatac aattactgta gctctagtta acgagttgaa caacattaag aacgagtatt
    20401 tgaataaaat agttgaatca acctcagaaa gccaactaca gatactgatg atggagagtc
    20461 gatcaaaagt agatgaagct gtgtctaagt ttgaaaagga ctcatcttct tcgtcaagtt
    20521 cagactcttc cactaaaccg gaagcttcag atacagcgaa gccaaacaag ccgacagaac
    20581 caggagaaaa ggtagcagaa gctaagaaga aggttgaaga agctgagaaa aaagccaagg
    20641 atcaaaaaga agaagatcgt cgtaactacc caaccattac ttacaaaacg cttgaacttg
    20701 aaattgctga gtccgatgtg gaagttaaaa aagcggagct tgaactagta aaagtgaaag
    20761 ctaacgaacc tcgagacgag caaaaaatta agcaagcaga agcggaagtt gagagtaaac
    20821 aagctgaggc tacaaggtta aaaaaaatca agacagatcg tgaagaagca gaagaagaag
    20881 ctaaacgaag agcagatgct aaagagcaag gtaaaccaaa ggggcgggca aaacgaggag
    20941 ttcctggaga gctagcaaca cctgataaaa aagaaaatga tgcgaagtct tcagattcta
    21001 gcgtaggtga agaaactctt ccaagcccat ccctgaaacc agaaaaaaag gtagcagaag
    21061 ctgagaagaa ggttgaagaa gctaagaaaa aagccgagga tcaaaaagaa gaagatcgcc
    21121 gtaactaccc aaccaatact tacaaaacgc ttgaacttga aattgctgag tccgatgtgg
    21181 aagttaaaaa agcggagctt gaactagtaa aagaggaagc taaggaacct cgaaacgagg
    21241 aaaaagttaa gcaagcaaaa gcggaagttg agagtaaaaa agctgaggct acaaggttag
    21301 aaaaaatcaa gacagatcgt aaaaaagcag aagaagaagc taaacgaaaa gcagcagaag
    21361 aagataaagt taaagaaaaa ccagctgaac aaccacaacc agcgccggct ccaaaagcag
    21421 aaaaaccagc tccagctcca aaaccagaga atccagctga acaaccaaaa gcagaaaaac
    21481 cagctgatca acaagctgaa gaagactatg ctcgtagatc agaagaagaa tataatcgct
    21541 tgactcaaca gcaaccgcca aaaactgaaa aaccagcaca accatctact ccaaaaacag
    21601 gctggaaaca agaaaacggt atgtggtact tctacaatac tgatggttca atggcgacag
    21661 gatggctcca aaacaatggc tcatggtact acctcaacag caatggcgct atggcgacag
    21721 gatggctcca aaacaatggt tcatggtact atctaaacgc taatggttca atggcaacag
    21781 gatggctcca aaacaatggt tcatggtact acctaaacgc taatggttca atggcgacag
    21841 gatggctcca atacaatggc tcat
//
LOCUS       DBDESG010000037        21811 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.037, whole genome shotgun sequence.
ACCESSION   DBDESG010000037 DBDESG010000000
VERSION     DBDESG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.037"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            123..344
                     /locus_tag="ACKB38_001344"
     CDS             123..344
                     /locus_tag="ACKB38_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980106.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            791..1093
                     /locus_tag="ACKB38_001345"
                     /pseudo
     CDS             791..1093
                     /locus_tag="ACKB38_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1249..2478
                     /locus_tag="ACKB38_001346"
     CDS             1249..2478
                     /locus_tag="ACKB38_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGL7980107.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2498..5194
                     /gene="ppc"
                     /locus_tag="ACKB38_001347"
     CDS             2498..5194
                     /gene="ppc"
                     /locus_tag="ACKB38_001347"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGL7980108.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5277..5494
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5607..6611
                     /gene="trpX"
                     /locus_tag="ACKB38_001348"
     CDS             5607..6611
                     /gene="trpX"
                     /locus_tag="ACKB38_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7980109.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6680..7546
                     /locus_tag="ACKB38_001349"
     CDS             6680..7546
                     /locus_tag="ACKB38_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980110.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7543..8301
                     /locus_tag="ACKB38_001350"
     CDS             7543..8301
                     /locus_tag="ACKB38_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980111.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8391..10151
                     /gene="dnaG"
                     /locus_tag="ACKB38_001351"
     CDS             8391..10151
                     /gene="dnaG"
                     /locus_tag="ACKB38_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGL7980112.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10154..11263
                     /gene="rpoD"
                     /locus_tag="ACKB38_001352"
     CDS             10154..11263
                     /gene="rpoD"
                     /locus_tag="ACKB38_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGL7980113.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11277..11606
                     /locus_tag="ACKB38_001353"
     CDS             11277..11606
                     /locus_tag="ACKB38_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGL7980114.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11721..12764
                     /gene="cpoA"
                     /locus_tag="ACKB38_001354"
     CDS             11721..12764
                     /gene="cpoA"
                     /locus_tag="ACKB38_001354"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGL7980115.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12780..14105
                     /locus_tag="ACKB38_001355"
     CDS             12780..14105
                     /locus_tag="ACKB38_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGL7980116.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14136..14312
                     /locus_tag="ACKB38_001356"
     CDS             14136..14312
                     /locus_tag="ACKB38_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGL7980117.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14371..14505
                     /locus_tag="ACKB38_001357"
     CDS             14371..14505
                     /locus_tag="ACKB38_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGL7980118.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14569..15879
                     /gene="obgE"
                     /locus_tag="ACKB38_001358"
     CDS             14569..15879
                     /gene="obgE"
                     /locus_tag="ACKB38_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGL7980119.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15889..16047
                     /locus_tag="ACKB38_001359"
     CDS             15889..16047
                     /locus_tag="ACKB38_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980120.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(16922..17047)
                     /locus_tag="ACKB38_001360"
     CDS             complement(16922..17047)
                     /locus_tag="ACKB38_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980121.1"
                     /translation="MKIKDQTRKLAAGCSKHCFEVVDGTDVVSILHIRQGDIDVI"
     gene            17190..18449
                     /locus_tag="ACKB38_001361"
     CDS             17190..18449
                     /locus_tag="ACKB38_001361"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGL7980122.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18465..19016
                     /locus_tag="ACKB38_001362"
     CDS             18465..19016
                     /locus_tag="ACKB38_001362"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980123.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            19009..20286
                     /gene="spxR"
                     /locus_tag="ACKB38_001363"
     CDS             19009..20286
                     /gene="spxR"
                     /locus_tag="ACKB38_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGL7980124.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20302..21162
                     /locus_tag="ACKB38_001364"
     CDS             20302..21162
                     /locus_tag="ACKB38_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGL7980125.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21387..21497
                     /locus_tag="ACKB38_001365"
                     /pseudo
     CDS             21387..21497
                     /locus_tag="ACKB38_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            21542..21811
                     /locus_tag="ACKB38_001366"
                     /pseudo
     CDS             21542..21811
                     /locus_tag="ACKB38_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         6682 a         3748 c         4886 g         6495 t
ORIGIN      
        1 aaaacttgat acataaaaat aaaagtctat ataaaggatg gtaaaattcc tgttgtccga
       61 tttggacaat atcctaaata gttacaatat atggtctata ctttttctta ggagaaagct
      121 agatgtacag acgtttgaga gatttgaggg aggatcatga tctgccccaa aagcaaatag
      181 ctacaatact ttcgtttaca aattcagctt atgccaaaat tgaacggggt gagcatgcgt
      241 tgacggctga tgtattggtt aaactctcag atttctatga cgtcagtaca gactatttat
      301 tgggattaac tgattttcct gataaaattc gctttagaaa ataatctcct caatttcata
      361 gagtttgaaa atgagtgaga ttttttattt gccctttgac aactgaatag cctaaaatgg
      421 tactttcctc atttgtggag caaatttgaa tggctcgcca tgataagagc gattttaaaa
      481 tcatcaataa aatagagcga tactttatat gccatgatac aaatgatata caatgatact
      541 tctgaccgtt cagcctgcca acgtaaaaga gcagcaagtg aaattcttat gatgacttca
      601 tcagtcatgc cacgttgaat gtgtgagttt gttagataaa cgcaattaat cctcaaaagg
      661 ttccccgaac cttttgagtt ctacagacgc atcacgtgga gtgtgtaagc ttgttgctaa
      721 aagcgtaaaa accttggaac gaaaggaata atagactttc tgcgaaacaa aaatataata
      781 caataaaact atgaatgatg aagcaagtaa acaattgagc gatagccgtt tcaagatcct
      841 tgtaggtgtt cagcgcacga cttttgaaga gatgttagct gtgttaaaaa cagcttatca
      901 acgtaaacgc gcaaaaggtg gacgaaaaag caaattaagc ctagacgatc tccttatggt
      961 aactattcaa tacatgcgag aatagagcac ttatgaacaa attgcggctg attttggcat
     1021 tcacgaaagc aacttaatcc gtcggagtca atgggttgaa gcaactctta ttcaaaatgg
     1081 ttttacgatt tcaaattctg ccttaattct gtaaaaacag taaaattcga aggattgtaa
     1141 ggtaagagtt tttttctttc tgaaaaaatg gtataatagc aatcaaaact agaaaataaa
     1201 acggaatttg gaacagattt gtctgtatcc tagtagagtg gtgatactat gaagattagt
     1261 aagaggcact tattaaatta ttccatcttg attccctact tgcttttatc tattttgggc
     1321 ttgattgtgg tctattcgac caccagtgct attttaattg aagaaggcaa gagcgccttg
     1381 cagttggttc gaaaccaagg aatcttttgg attgttagtt tgatactgat tgccttaatt
     1441 tataaattga gactagattt tttgagaaat gagcgactaa tcattttagt tatattaata
     1501 gaaatgcttt tattgttctt ggctcgtttt attggtattt ccgtaaacgg ggcatacggt
     1561 tggatttcgg ttgcaggaat aactattcag ccagctgagt acttaaaaat cattattatt
     1621 tggtatttag ctcaccgatt ctccaaacag caagaagaaa tagctactta tgattttcaa
     1681 gttttgactc aaaatcaatg gcttccccgt gcttttaatg attggcgatt cgttctccta
     1741 gttctgattg gaagtttggg aattttccct gatttaggaa atgcgactat tttagtcttg
     1801 gtttccttga ttatgtatac agttagtgga atcgcttatc gctggttttc aaccattctg
     1861 gcgctcgtat ctgccgcttc tgtctttgtc ttgaccacta tcagcctaat cggtgttgag
     1921 accttttcaa aaattccagt attcggctat gtagccaagc gctttagtgc cttttttaat
     1981 ccttttgccg atcgtgctga tgcaggtcac cagttagcta attcttattt tgccatggtc
     2041 aatggcggtt ggtttggtct aggtcttgga aactcgattg aaaaacgagg ttatttgcca
     2101 gaagctcata cagactttgt cttttctatc gtgattgaag aatttggctt tgttggtgcc
     2161 agtcttattt tagctctctt gtttttcatg attttgcgga ttatcttggt cggtatccga
     2221 gcggagaatc ctttcaatgc catggttgca ctcggtgtcg gagggatgat gttggttcag
     2281 gtatttgtca atatcggagg gatttcgggc ttgattccat ctacaggagt gactttcccc
     2341 ttcttatccc agggtggaaa tagtcttcta gtcttatcag tggcagtagc ctttgtctta
     2401 aatattgatg ccagtgaaaa acgcgctaaa ttgtaccgag aattggaaaa tcaaccaatg
     2461 aaccttctgt tgaagtagga taaagaaagg atagtttatg tctcttcaaa aattagaaaa
     2521 ttatagtaat aaaagtgttg tgcaagaaga agtcttgatt ctaacagaat tactggaaga
     2581 tattactaaa aatatgcttg ccccagagac ctttgaaaaa ataatacagt tgaaagaatt
     2641 atcaacgcag gaagattatc aaggtctaaa ccgtctagtg actagcttat caaatgatga
     2701 aatggtctat atttcacgct atttctctat cttgcctctt ttgattaata tttcagagga
     2761 tgtggattta gcttatgaaa tcaatcatca aaataatatt gatcaggact atttaggtaa
     2821 attatctaca acgattaaat tggtagcaga aaaggaaaat gccgttgaga tcctagaaca
     2881 cttgaatgtt gtccctgttt tgacagccca tccaacacaa gtgcaacgca aaagtatgtt
     2941 ggatttaaca aatcatattc atagtctttt gcgtaaatac cgtgatgtta agttggggtt
     3001 gatcaataaa gataaatggt acaatgattt gcgtcgttac atcgaaatta tcatgcagac
     3061 agacatgatt cgtgagaaaa aattaaaagt gactaacgaa atcacgaatg ctatggaata
     3121 ttataacagc tcctttttga aagctgtacc tcatttgacg acggagtata agcgcttagc
     3181 gcaagcgcat ggtctgaatt taaaacaggc taaaccaatc accatgggta tgtggatagg
     3241 tggtgaccgt gatggaaatc catttgttac agcaaagacc ttgaagcagt ctgcactcac
     3301 tcagtgtgaa gtcatcatga actactatga taaaaagatt taccaacttt atcgtgaatt
     3361 ttctctttca actagcattg tcaacgtcag caagcaagtc agagaaatgg ctcgtcaatc
     3421 caaggataac tcgatttacc gcgaaaaaga gctttaccgt cgtgccttgt ttgatattca
     3481 atcaaaaatt caggcaacta aaacctatct gattgaggat gaagaagttg ggactcgtta
     3541 tgaaaccgcc aatgatttct acaaggattt gattgccatt cgagattctc tactagaaaa
     3601 taagggcgag tccttgattt caggtgattt tgtggaatta ttgcaggcag tagagatatt
     3661 tggtttttac ttagcatcaa ttgatatgcg acaagactct agcgtctatg aagcctgtgt
     3721 ggcagaactc ttgaaatcag caggaattca ttctcgttat agcgagttga gcgaagaaga
     3781 aaagtgtgac cttctcttga aagaattaga agaagatccc cgaattcttt ctgcgactca
     3841 cgcagaaaaa tcagaattat tagcaaaaga attagctatt tttaagacgg ctcgtgtttt
     3901 gaaagataag ttgggagatg atgtcatccg tcagaccatc atttcacatg caaccagcct
     3961 ttctgatatg ctagaattag ctattctgtt aaaagaagta ggactggtgg atacggaaag
     4021 ggcgcgtgtt cagattgttc ccctttttga aacaattgaa gacttggatc attcagagga
     4081 aacaatgaga aaatatcttt ctcttagcct tgccaaaaaa tggattgact cacgaaataa
     4141 ctaccaagaa atcatgcttg gctactctga cagtaataaa gatggcggtt acttgtcatc
     4201 atgttggacc ctctacaagg ctcaacaaca attgactgct attggagatg aatttggcgt
     4261 taaggttacc ttcttccatg gtcgtggtgg tactgtcggt cgtggtggtg ggccaaccta
     4321 tgaagccatt acatctcaac cgctcaagtc tatcaaggat cgtatccgct tgacggagca
     4381 gggtgaagta attgggaata aatacggtaa caaagacgcc gcttactata accttgaaat
     4441 gctagtatcg gcagctatta accgtatgat tactcagaag aagagcgata ccaatacccc
     4501 aaatcgttat gaaaccatta tggatcaagt agtggaccgt agttacgata tctaccgtga
     4561 tttggtcttt ggtaatgagc atttctatga ttatttcttc gagtcaagtc caatcaaggc
     4621 tatttcaagt tttaatattg gttctcgtcc agccgctcgt aagactatta ctgaaatcgg
     4681 tggtttgcgt gccatccctt gggtattctc atggtcacag agtcgtgtta tgttccctgg
     4741 atggtacggg gttggttcaa gcttcaagga atttatcaat aaaaatccag agaatattgc
     4801 tatcttacga gatatgtacc aaaattggcc tttcttccaa tcgcttcttt caaatgttga
     4861 tatggttttg tcaaaatcaa atatgaatat tgcttttgaa tatgctaaac tttgtgaaga
     4921 cgagcaagtt aaggccatct atgagactat tttaaatgaa tggcaagtta ctaagaacgt
     4981 tatcttggct attgaaggac atgacgaact cttagctgac aatccatatc taaaagctag
     5041 tctggattac cgtatgcctt actttaatat tctcaactat attcagttgg agttgattaa
     5101 acgccaacgt cgtggagaat tgtccagtga tcaagaacga ttgattcata tcaccatcaa
     5161 cggaattgcg acaggattgc gtaattcagg ttgataattt tcaagagtga atgctaaaag
     5221 tgaatatcaa aaaaattcta atagactatt gacaagtagt ttaaaaatga tataatttaa
     5281 ccattcagaa aagtaatcat acaaactttt tagagagtct gtggtagctg aaaacagata
     5341 agtggcaatg atgaaaattg ggctgaatgc tatttagaat ttgaaattat aaaaattcgg
     5401 taagcacacc ttacagtgca tctcgttatt gcgagactga gcgataggga aattccctat
     5461 aattgaggtg gtaccgcgca tcgacgtcct cacacaagtt ttttgtgtga ggattttttt
     5521 gatggaggtt agtatggaaa gaaaacgatg gcgtcgcttg tttagataag tgaaatatgt
     5581 taaaggaaat aaaaaggaga aacagaatga aaaataaacg tttaattgga attattgctg
     5641 cattagcagt cttagtagca ggaagcttga tttattcttc aatgaataaa tcagaagctc
     5701 agaataataa ggatgagaag aaaataacca agattggtgt gcttcaattt gtgagccatc
     5761 catcccttga tttgatttat aaagggatcc aagatggact tgcagaagaa ggatataaag
     5821 atgatcaagt taaaattgat tttatgaact cagaaggtga ccaaagtaag gttgcgacaa
     5881 tgagtaaaca attggttgca aatgggaatg accttgtggt tggtatcgca acaccagcag
     5941 cccaagggtt ggctagtgca acaaaagacc taccggttat catggccgct attacagacc
     6001 caattggtgc taacttggtt aaagatttga aaaaaccagg tggcaacgtt acaggggtat
     6061 ctgaccacaa tccagctcaa caacaagttg aactcatcaa ggctctgaca ccgaatgtga
     6121 aaacaatcgg agctctttac tcaagtagcg aagacaattc aaaaacacag gtcgaagaat
     6181 ttaaggctta tgctgaaaaa gcaggtctga cagtggaaac atttgcagtt ccttcaacaa
     6241 atgaaattgc ctcaactgtc actgttatga ctagcaaggt agatgctatt tgggttccaa
     6301 ttgataacac cattgcatca ggatttccaa cggttgtctc tagcaatcaa agttctaaga
     6361 aaccaattta tcccagtgcg acagctatgg tagaagtagg tggtttggca tcagttgtaa
     6421 ttgaccaaca tgaccttggt gtggcaacag gtaaaatgat tgtgcaagtc ttgaaaggtg
     6481 caaaaccagc cgatacccca gtcaatgtct tttcaactgg taagtcagtc atcaataaaa
     6541 aaatagcaca agaactaggt attactattc ctgagtctgt tctcaaagaa gcaggacaag
     6601 tcatcgaata aataaggtag ggaggaggag actcttccca ttttttaaaa agatgaatat
     6661 agaaaggatt aaataaaata tgattgtttc cattatttct caaggatttg tctgggctat
     6721 tctaggtctg ggaatcttta tgacatttag gattttaaac tttccagata tgacgacaga
     6781 aggttccttc cctcttgggg gagctgttgc tgtcactttg ataaccaaag gcgtgaaccc
     6841 atttttagcg acacttgttg ctgtaggagc aggttgtttg gctggaatgg cagcaggcct
     6901 tctttataca aaagggaaga tcccaacctt gctctcaggg attttggtga tgacttcttg
     6961 tcactcaatc atgctcttga ttatgggacg tgcgaattta ggcctgcttg gaaccaagca
     7021 aattcaggat gttttgcctt ttgattcgga tttgaatcaa ctcttgacag gtctcatctt
     7081 tgtgagtatt gttattgctc tcatgctctt tttcttggac actaaactcg gacaagccta
     7141 tattgctaca ggggataatc ctgatatggc tagaagtttc gggattcata ctggacgcat
     7201 ggagctcatg ggcttggtct tatcaaatgg tgtgattgcc cttgcaggtg ccctcattgc
     7261 tcagcaagaa ggttatgccg atgtgtctcg agggatcggg gttatcgttg tggggcttgc
     7321 aagtttgatt attggagaag ttattttcaa gagtttgagc ttggcagagc gtttggttac
     7381 tatcgttgta ggttctatcg cttatcaatt tttagtgtgg gcagttatcg cacttggctt
     7441 taatacaagt taccttcgtt tatacagtgc cttgatttta gcagtctgcc tcatgattcc
     7501 aacatttaag caaacaatct tgaaaggagc caagttaagc aaatgacagc aattgtagaa
     7561 ttaaaaaatg caaccaaaat cgttaaaaat ggctttgatg aagaaaagat tattttaaat
     7621 gatgtttcct tagaaatttt tgaacgggac tttatcacga ttttgggcgg aaatggtgct
     7681 ggaaaatcaa ctctctttaa cactatagca gggaccttat cactaactag tggaactatc
     7741 cgtattttag gtgaagatct cactaagttt tcacccgaga agcgtgccaa gtacctgtct
     7801 cgtgtcttcc aagatccaaa gatggggaca gctccccgta tgacggtcgc tgaaaatctt
     7861 ttaatcgcca agtttcgtgg tgaaaagcgt ggattgttac cacgacgctt gactagctat
     7921 aaggatgaat ttcaggcaac cattgaaaaa gtaggaaatg gtcttgagaa acacttgaat
     7981 acaccgattg agttcttatc aggtggacaa agacaggctt tgagtctctt gatggcaacc
     8041 ttgaagcgac ctgaattact cctgttagat gagcatactg ctgccctgga tccaaagact
     8101 agtgttgctt tgatggaatt gacagatgaa tttgttaaga aagatcagct aacagccctt
     8161 atgattactc atcatatgga agatgctctc aaatacggca atcgcttgat tgtcatgaaa
     8221 gaaggacgaa ttatccaaga tttaaaccaa gaagaaaaag caaaaatgaa aatctctgat
     8281 tattatcaac tctttgaata aagtagggaa aatgagtgaa atggtttact ttttttctga
     8341 aataaagtat actatataaa gtaaactatg ataacatgga ggtattgtgt atggttgaca
     8401 aacaagtcat tgaagaaatc aaaaacaatg ccaacattgt ggaagtcata ggagatgtga
     8461 tttctttaca aaaggcagga cggaactatc tagggctctg tccttttcat ggtgaaaaaa
     8521 caccatcttt caatgttgta gaggacaagc agttttatca ctgtttcggt tgtggtcgct
     8581 caggtgatgt ctttaagttc atcgaggagt accaaggggt tccctttata gaagcagtcc
     8641 aaatcttagg tcagcgtgtt ggaattgagg ttgaaaaacc gctttatagt gagcagaagt
     8701 cagcctcacc tcaccaagct ctttatgata tgcacgaaga tgcagccaaa ttttaccatg
     8761 ctattctcat gacaacgact atgggcgaag aggccagaaa ttacctttat cagcgggggt
     8821 tgacagatga agtgcttaaa catttttgga ttggtttagc acctccagaa cgaaactatc
     8881 tctatcaacg tttgtctgat cagtatcgtg aagaggattt actggattca ggcctgtttt
     8941 atctctcgga tgccaatcaa tttgtagaca cctttcacaa tcgcattatg tttcccctga
     9001 caaatgacca aggaaaggtc attgccttct caggtcgtat ctggcaaaaa acggattcac
     9061 aaacttctaa gtataaaaac agccgatcga ctgcaatttt taacaaaagt tacgaattat
     9121 atcatatgga tagggcaaaa agatcttctg gaaaagctag tgagatttac ctgatggaag
     9181 gattcatgga tgttattgca gcctatcggg ctggaatcga aaatgctgtg gcgtcgatgg
     9241 gaacagcctt gagtcgagag catgttgagc atctgaaaag gttaaccaag aaattggttc
     9301 ttgtttacga tggagataag gctgggcaag ccgcgacatt gaaagcattg gatgaaattg
     9361 gtgatatgcc tgtgcaaatc gtcagcatgc ctgataactt ggatcctgat gagtatctac
     9421 aaaaaaatgg tccagaagac ttggcctatc tattaacgaa aactcgtatt agtccgattg
     9481 agttctacat tcatcagtac aaacctgaaa acagtgaaaa tctgcaggct cagattgagt
     9541 ttcttgaaaa aatagctccc ttgattgttc aagaaaagtc catcgctgct caaaacagct
     9601 atattcatat tttagctgac agtctggcgt cctttgatta tacccagatt gagcagattg
     9661 ttaatgagag tcgtcaggtg caaaggcaga atcgcatgga aggaatttcc agaccgacgc
     9721 caatcaccat gcctgtcacc aagcagttat cggctattat gagggcagaa gcccatctac
     9781 tctatcggat gatggaatcc cctcttgttt tgaacgatta ccgtttgcga gaagactttg
     9841 catttgctac acctgaattt caggtcttat atgacttgct tggccagtat ggaaatcttc
     9901 ctccagaagt tttagcagag cagacagagg aagttgaaag agcttggtac caagttttag
     9961 ctcaggattt gcctgctgag atatcgccgc aggaacttag tgaagtagag atgactcgaa
    10021 acaaggctct cttgaatcag gacaatatga gaatcaaaaa gaaggtgcag gaagctagcc
    10081 atgtaggaga tacagataca gccctagaag aattggaacg tttaatttcc caaaagagaa
    10141 gaatggagta ataatggcaa caaaacaaaa agaagtaaca acatttgacg tacaggtagc
    10201 agaatttatc cgtaatcata agcaaaaagg gacagcaaca gatgatgaaa tcaatgcaag
    10261 tctggttatt ccttttacct tggacgctga tgggattgaa gatctcttgc aacggattca
    10321 ggatgcaggc atttctatca cagataacga aggaaatcca agtgcgcgtg ttctcagcaa
    10381 tgaagaagaa ccagaactca gcgatgagga cttgattggg tcaacttctg ctaaggtcaa
    10441 tgaccctgtc cgtatgtact tgaaagaaat aggggtcgtt cctctcttga ccaatgaaga
    10501 ggagaaagag ttggcactgg ctgttgaagc tggtgatatc gaagccaaac aacgtcttgc
    10561 ggaagccaat cttcgtttgg ttgtttccat tgccaaacgc tatgtcggtc gtggtatgca
    10621 gttccttgac ttgattcaag aaggaaatat gggcttgatg aaggcggttg acaagtttga
    10681 ctattctaaa gggttcaagt tttcaactta tgcaacttgg tggattcgtc aggctatcac
    10741 tcgtgctatt gcggaccaag ctcgtaccat ccgtatccca gttcacatgg ttgaaactat
    10801 caataaattg gttcgtgaac agcggaatct ccttcaagaa ttggggcaag atccgacacc
    10861 agaacagatt gctgaacgaa tggatatgac acctgataag gttcgtgaaa tcttgaagat
    10921 tgcccaagaa ccagtatctc ttgaaactcc tatcggtgaa gaggacgata gccaccttgg
    10981 agactttatc gaagatgaag tgattgaaaa tccagtggat tatacgactc gtatcgtctt
    11041 gcgtgagcaa ttggatgaaa tcttagatac tcttacagac cgtgaagaaa atgttctgcg
    11101 tctacgtttt ggactagatg atggaaaaat gcgcacactt gaagatgtgg ggaaagtctt
    11161 taacgtaact cgtgagcgta tccgtcagat tgaagcaaag gctttgagaa aactacgcca
    11221 accaagtcgt agcaaaccgc ttcgtgattt tattgaagac taagagtgag gaaaatatgg
    11281 cttatacaga agagcaaatt gaaaacatca aaacacggat tttaacagcc ttggaagaag
    11341 tcatcgaccc tgagttggga atcgatattg tcaatcttgg tttgatctat gagattcgtt
    11401 ttgacggtga cacagggcaa acagagattg acatgacttt gacaactatg ggttgtccct
    11461 tagcagacct tttgacagac cagatttatg atgctatgat agaggttcca gaagtaacgg
    11521 atactgaggt taaattagtc tggtatccag cctggacagt tgaaaaaatg agtcgctatg
    11581 cccgcattgc cctaggcatt aagtaaacat ataaaagcat gtgagagact gttggaaaag
    11641 cgaggaaatt tcccctcttt tcctctagtc tctcctttct tttgctgatt ttattcaaag
    11701 aaaatgatat aatagtagtt atggagaaaa agaaattacg catcaatatg ttgagttcaa
    11761 gtgagaaagt agcaggacag ggagtttcag gtgcttaccg tgaattagtt cgtcttcttc
    11821 accgtgctgc caaggaccaa ttgattgtta cagaaaatct tccaatcgag gcagatgtga
    11881 ctcactttca tacgattgat tttccctatt atttatcaac cttccaaaag aaacgctcag
    11941 ggagaaagat tggctatgtg catttcttgc cagctacact tgagggaagt ttgaaaattc
    12001 catttttctt aaagggaatt gtgaaacgct atgtattttc tttttacaac cggatggagc
    12061 acttggttgt ggtcaatcct atgtttattg aggatttggt agcagctggt attccacgtg
    12121 aaaaagtgac ctatattcct aactttgtca acaaggaaaa atggcatcct ctaccacaag
    12181 aagaggtagt cagactgcgc acagatcttg gtcttagtga caatcagttt atcgtagtag
    12241 gtgctgggca agttcagaaa cgtaaaggga ttgatgactt tatccgtctg gctgaggaat
    12301 tgcctcagat tacctttatc tgggctggtg gcttctcttt tggtggtatg acagatggtt
    12361 atgaacacta taagaaaatt atggaaaatc cccctaaaaa tttgattttt ccaggcattg
    12421 tatcgccaga gcggatgcgc gaattgtatg ctctagcgga tcttttcttg ttgcctagtt
    12481 acaatgagct ctttcctatg actattttag aagctgcgag ttgtgaggct cctattatgt
    12541 tgcgtgattt agatctctat aaggtgattt tggagggaaa ttatcgggcg acagcgggta
    12601 gagaagagat gaaagaggct attttggaat atcaagcaaa tcctgctgtc ttaaaagatc
    12661 tcaaagaaaa ggctaagaat atttccagag agtattctga agagcatctg ttacaaatct
    12721 ggttggactt ttatgagaaa caagccgctt tagggagaaa gtaaaaagtg aggtaatcta
    12781 tgcgaattgg tttatttaca gatacctatt ttcctcaggt ttctggtgtt gcgaccagta
    12841 ttcgaacctt gaaaacagaa cttgaaaagc agggacatgc tgtttttatc tttacgacga
    12901 cagataagga tgtcaatcgc tacgaagatt ggcaaattat ccgcattcca agtgttcctt
    12961 tctttgcttt taaggatcgt cgctttgcct accgaggttt tagcaaggca cttgaaattg
    13021 ctaaacagta tcagctagat attatccata ctcagacaga attttctctt ggcctgttgg
    13081 ggatttggat tgcgcgtgaa ttgaaaattc cagtcatcca tacctatcac acccagtatg
    13141 aagactatgt ccattatatt gctaagggga tgttgatccg gccgagtatg gtcaagtatc
    13201 tggttagagg tttcctgcat gatgtggatg gggttatttg ccctagtgag attgtccgtg
    13261 acttgctatc tgattataag gtcaaggttg aaaaacgggt cattcctact gggattgaat
    13321 tagccaagtt tgagcgtccg gaaatcaagc aggaaaattt gaaagaactg cgtagtaaac
    13381 tagggattca agatggtgaa aagacgttgc ttagtctttc gagaatctcc tatgaaaaaa
    13441 atattcaagc agttttagca gcctttgctg atgttctgaa agaggaagac aaggttaaac
    13501 tggtagtagc tggggatggc ccttatctga atgacctcaa agagcaagcc cagaacctag
    13561 agattcaaga ctcagtcatc tttacaggga tgattgctcc tagtgagacg gctctttact
    13621 ataaagcggc ggatttcttc atttcggcat cgacaagcga aacgcaaggt ttgacctact
    13681 tggaaagctt agccagtgga acacctgtca ttgctcacgg aaatccttat ttgaacaacc
    13741 tcatcagtga taaaatgttt ggaaccttgt actatggaga acatgatttg gctggtgcta
    13801 ttttggaagc cctgattgca acaccagaca tgaacgagca taccttatca gagaaattgt
    13861 atgagatttc agctgagaac tttgggaaac gagtgcatga gttttatctg gatgccatta
    13921 tttcaaataa cttccagaaa gatttggcta aagatgatac ggtcagtcag cgtatcttta
    13981 agacagtttt gtatcttcag caacaggtgg ttgctgtacc tgtaaaagga tctagacgca
    14041 tgttgaaggc ttcaaaaaca cagttgatca gtatgagaga ctattggaaa gaccatgaag
    14101 aatagaaaga ggaacagcta tgaaaaaaac aattaatgag aagcggtcgt gataaaaaga
    14161 ttgcgggtgt ttgtgctggg gtggcccatt atctggatat ggatccgact atcgttcaag
    14221 tcatttgggg tgttcttact tgctgttacg gagctggaat tgtagcttac attattttat
    14281 ggattatcgc accagtagca actgactatt gaaaactagc tcaattcatg ctaagataat
    14341 agaaaataga atgtaacgaa ggagagaaaa atggcatttg gagataatgg aaatcgtaaa
    14401 aaaactatgt ttgagaaaat aaccttgttt atcgtgatta tcatgctagt agcaagttta
    14461 ttgggaattt ttgcaactgc aattggtgcc ctcagtaatc tataaaatag attcaagaaa
    14521 atttagtgac tgggatttcc cagccctttt ttaaagtgag aagaaataat gagtatgttt
    14581 ttagatacag ctaagattaa ggtcaaggct ggtaatggtg gcgatggtat ggttgccttt
    14641 cgtcgtgaaa aatatgtccc taatggaggc ccttggggtg gtgatggtgg tcgtggaggc
    14701 aatgtggtct tcgttgtaga cgaaggacta cgtaccttga tggatttccg ctacaatcgt
    14761 catttcaagg ctgattctgg tgaaaaaggg atgaccaaag ggatgcatgg tcgtggtgct
    14821 gaggacctta gagttcgagt accacaaggt acgactgttc gtgatgcgga gactggcaag
    14881 gttttaacag atttgattga acatgggcaa gaatttatcg ttgcccacgg tggtcgtggt
    14941 ggacgtggaa atattcgttt cgcgacacca aaaaatcctg caccggaaat ctctgaaaat
    15001 ggagaaccag gtcaggaacg tgagttacaa ttggaactaa aaatcttggc agatgtcggt
    15061 ttagtaggat tcccatctgt agggaagtca acacttttaa gtgttattac ctcagctaag
    15121 cctaaaattg gtgcctacca ctttaccact attgtaccaa atttaggtat ggttcgcacc
    15181 caatcaggtg aatcctttgc agtagccgac ttgccaggtt tgattgaagg ggctagtcaa
    15241 ggtgttggtt tgggaactca gttcctccgt cacatcgagc gtacacgtgt tatccttcac
    15301 atcattgata tgtcagctag cgagggccgt gatccatatg aggactacct agctatcaat
    15361 aaagagctgg agtcttacaa tcttcgcctc atggagcgtc cacagattat tgtagctaat
    15421 aagatggaca tgcctgagag tcaggaaaat cttgaagact ttaagaaaaa attggctgaa
    15481 aattatgatg aatttgaaga gttaccagct atcttcccaa tttctggatt gaccaagcaa
    15541 ggtctggcaa cacttttaga tgctacagct gaattgttag acaagacacc agaatttttg
    15601 ctctacgacg agtccgatat ggaagaagaa gcttactatg gatttgacga agaagaaaaa
    15661 gcctttgaaa ttagtcgtga tgacgatgcg acatgggtac tttctggtga aaaactcatg
    15721 aaactcttta atatgaccaa ctttgatcgt gatgaatctg tcatgaaatt tgcccgtcag
    15781 cttcgtggta tgggggttga tgaagccctt cgtgcgcgtg gagctaaaga tggggatttg
    15841 gtccgcattg gtaaatttga gtttgaattt gtagactagg agactggtat gggagataaa
    15901 ccgatatctt tccgagatgc ggatggtaat tttgtttccg ccgcagacgt ttggaatgaa
    15961 aagaaattgg aagaactatt taatcgtctc aatccaaatc gtgccttgag attggcacga
    16021 actaaaaagg aaaatccatc tcagtaaaga agctaaaaaa tcccgtgcct catcagacac
    16081 gggattttgt ggtacgacag gcatgtatag caaactgaat ctggaatagc acagcatatc
    16141 ttctaaaata tagtaaaatg aaatgagaac aggacaaatc gatcaggaca gtaaaatcga
    16201 tttctaacaa tgttttataa gcagagatgt actattctag tttcaatcaa ctatattgtt
    16261 ataaattgat ttgaatttca aaattaaatt gtttgattct tatttcaatt tgttatagta
    16321 tatctgatgt caaagttctc ggcgagtcaa atagcgattc ccaagcctga ctatcgtgag
    16381 gtagcggatt aaaatggtct ggggatagac cgttttaagt ctgacgctgg aaataagaat
    16441 tgtcagaaga agggatagcg aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt
    16501 atatagcgga taagagggca tcaaactcta aagtccaaaa aggtagtcgt aacctatatg
    16561 cgtaaatcac gagagtaatt gaattcgtac taagattttc tattttcact gtaacctttt
    16621 aacgccctta tatcttgtat acacgaggaa agatgtacga cttatcccgt gaggtctatc
    16681 actataaaga gaaaacgaca gatagaagtg atcctgagtc acggttatct gtctgatagg
    16741 acggtatgta taaaacgctt ctgtgaactg agagaagggg gagaagttct tgctaaaatt
    16801 tagttgaaca gccgtattcc gatacttaga taagagatct agtcttagct cctactcagt
    16861 tttaggggat aaaaaagggg caatagcgat tcgagaaaga ttatactctt cgaaaatctc
    16921 ttcaaatcac gtcaatatcg ccttgtcgta tgtgtaggat actgactacg tcagttccat
    16981 ctacaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt tgatctttga
    17041 ttttcattga gtattagtaa ttcagttact aactcgtcaa ctctgattta tccaataaaa
    17101 ttgaaaagga tggaaaaaag gataaattta tgatatactt tattttgaag accttattag
    17161 aaatcttgaa agagtattga aaacttagaa tgagaaaaat tgttatcaat ggtggattac
    17221 cactgcaagg tgaaatcact attagtggtg ctaaaaatag tgtcgttgcc ttaattccag
    17281 ctattatctt ggctgatgat gtggtgactt tggattgcgt tccagatatt tcggatgtag
    17341 ccagtcttgt cgaaatcatg gaattgatgg gagctactgt taagcgttat gacgatgtat
    17401 tggagattga cccaagaggt gttcaaaata ttccaatgcc ttatggtaaa attaacagtc
    17461 ttcgtgcatc ttactatttt tatgggagcc tcttaggccg ttttggtgaa gcgacagttg
    17521 gtctaccggg aggatgtgat cttggtcctc gtccgattga cttacacctt aaggcgtttg
    17581 aagctatggg tgccactgct agctacgagg gagataacat gaagttatct gctaaagata
    17641 caggacttca tggtgcaagt atttacatgg atacggttag tgtgggagca acgattaata
    17701 cgatgattgc tgcggttaaa gcaaatggtc gtactattat tgaaaatgca gcccgtgaac
    17761 ctgagattat tgatgtagct actctcttga ataatatggg tgcccatatc cgtggggcag
    17821 gaactaatat catcattatt gatggtgttg aaagattaca tgggacacgt catcaggtga
    17881 ttccagaccg cattgaagct ggaacatata tatctttagc tgctgcagtt ggtaaaggaa
    17941 ttcgtataaa taatgttctt tacgaacacc tggaagggtt tattgctaag ttggaagaaa
    18001 tgggagtgag aatgactgta tctgaagaca gcatttttgt cgaggaacag tctaatttga
    18061 aagcaatcaa tattaagaca gctccttacc caggctttgc aactgatttg caacaaccgc
    18121 ttacccctct tttactaaga gcgaatggtc gtggtacaat tgtcgatacg atttacgaaa
    18181 aacgtgtaaa tcatgttttt gaactagcaa agatggatgc ggatatttcg acaacaaatg
    18241 gtcatatttt gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa gcgaccgact
    18301 taagagctgg ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa actgaaatta
    18361 ccaatatcga gtttatctta cgtggttatt ctgatattat cgaaaaatta cgtaatttag
    18421 gagcggatat tagacttgtt gaggattaaa ccgtagaggt gtttatgaat atttggacca
    18481 aattagcaat gttttctttt tttgaaacgg atcgcttgta tttgcgtcct ttctttttta
    18541 gtgatagtca ggacttccgc gagatagctt caaatccaga aaatcttcaa tttattttcc
    18601 caacgcaggc aagtctggaa gaaagtcaat atgcactggc caattacttt atgaagtccc
    18661 ctttgggagt gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt tctattaaat
    18721 ttgagaagtt agatgaaatc aaaaaagaag ctgagcttgg ctattttttg agaaaagatg
    18781 cttggtcgca aggatttatg acagaggttg ttagaaaaat ttgtcagctt tcttttgagg
    18841 aatttggctt aaaacaatta tttatcatta cccaccttga aaataaagct agccaaagag
    18901 ttgctcttaa gtctggattt agtttgttcc gtcagtttaa gggaagtgat cgttacacaa
    18961 gaaaaatgcg ggattatctt gaatttcggt atgtaaaagg agagttcaat gagtaagcat
    19021 caggaaattc taagctattt ggaggaatta ccagtaggta aaagggtcag tgttcgtagc
    19081 atttcgaatc atctaggagt tagtgatgga acagcctatc gggctattaa agaagctgaa
    19141 aaccgtggaa ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa atcccagaaa
    19201 gttgctatag agagattaac gtttgctgaa attgcagaag tgacttcttc tgaggttctg
    19261 gctgggcaag aaggtttaga gagagaattt agtaagtttt caattggtgc catgactgaa
    19321 caaaatatct tgtcttacct tcatgatggg gggctcttga ttgtcggaga ccgaacccgt
    19381 attcagttgc tagccttgga aaatgaaaat gcagttctgg ttacaggggg atttcaggtt
    19441 catgatgatg tgcttaaact ggccaatcaa aaagggattc ctgttctaag aagtaagcat
    19501 gataccttta ccgtcgcgac catgatcaat aaagccttgt caaatgtcca aatcaagact
    19561 gatattctga cagttgagaa actttatcgc cctagtcatg agtatggttt tctgagagag
    19621 acagatacag ttaaagatta tttggacttg gttcgtaaga atcgtagcag ccgtttccct
    19681 gttatcaatc aacatcaggt cgttgttggt gttgtaacca tgagagacgc tggtgataaa
    19741 tcaccaagca cgacaattga taaggttatg tctcgtagtc tatttttggt tggattatcg
    19801 acaaatattg ccaatgtgag tcaacggatg atcgcagaag actttgaaat ggtaccagtt
    19861 gttcgaagca atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat ggagaagatg
    19921 agccgttccc aagtttcggc tctaccaact ttttctgagc agattggaca aaagctctct
    19981 tatcaccatg atgaagtagt cattacagtg gaacccttta tgctagaaaa aaatggagtt
    20041 ttggctaatg gtgtattggc agaaattctg acccacatga cccaagattt agttgttaat
    20101 agtggtcgca atctcattat cgagcagatg ctgatctact ttttgcaggc tgttcagata
    20161 gatgatatat tgcgcattca ggcacggatt attcatcata cgagacggtc agctataatt
    20221 gattacgata tttatcatgg tcaccagatt gtttcaaaag caaatgtgac tgttaaaatt
    20281 aattagaaac taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat cgaagctatg
    20341 gacaaggctg gtgattttct agcaagtatt catataggct tacgtgattt gattaagcca
    20401 ggcgtagata tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga agaaaatttc
    20461 cttccacttc agattggggt tgacggtgcc atgatggact atccttatgc tacctgttgc
    20521 tctcttaacg atgaagtggc tcacgctttc cctcgtcatt atatcttgaa agatggtgat
    20581 ttgctcaaag ttgatatggt tttgggaggt cccattgcta aatctgacct aaatgtctca
    20641 aaattaaact tcaacaatgt tgaacaaatg aaaaaataca ctcagagcta ttctggtggt
    20701 ttagcagact catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt caaaaacttg
    20761 atggatgtaa ccaaagaagc tatgtacaag ggtattgagc aagctgttgt tggaaatcgt
    20821 atcggtgata tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta cggtgtagtg
    20881 cgtgatttgg ttggtcatgg tgttggccca actatgcacg aagaaccaat ggttcctaac
    20941 tatggtattg caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac cattgaacca
    21001 atgatcaata caggcgattg ggaaattgat acagatatga aaactggttg ggcgcataag
    21061 accattgacg gtggattgtc atgtcagtat gaacaccaat ttgtcattac gaaagatgga
    21121 cctgttatct tgactagcca aggtgaagaa ggaacttatt aataaaaagt gaaaagacta
    21181 ctggaagttt attttgataa aaaatccagt agatcttttc ataataaaac gcattgtatc
    21241 aagtgttagg ggctgatatc atgcgttttt ctgcttttaa gattttttcc aactctgttt
    21301 gtaagcgcat cataacaaag ggtctaggat tcagggctct cctcctatat actattagta
    21361 aagtaaaact aagggaggat attttagtgt cgcagtctat tgttcctgta gagattccac
    21421 aatattgtcg ttttgattct aaaaagagaa atggaattct gtttaatgtt cgtattgcca
    21481 atcttaaatt tactttttta tattatactt cctgcgaaac aaaatatggt atagtagttc
    21541 tatgaatgat gaagcaagta aacaactaac tgatgcacga tttaagcgtc ttgttggtgt
    21601 tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
    21661 cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgc ccactcttca
    21721 atagtgcgag aatatcgaac ttatgaagaa attgcggctg attttggtat tcacgaaagc
    21781 aactttatcc gtcggagcca atgggttgaa a
//
LOCUS       DBDESG010000038        21799 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.038, whole genome shotgun sequence.
ACCESSION   DBDESG010000038 DBDESG010000000
VERSION     DBDESG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.038"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            153..308
                     /locus_tag="ACKB38_001367"
     CDS             153..308
                     /locus_tag="ACKB38_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980126.1"
                     /translation="MEKLQFFLSQRACGCWKQAEKAVKLGSSETREENIILKGVPFIA
                     QPVTGDI"
     gene            443..1804
                     /gene="trpE"
                     /locus_tag="ACKB38_001368"
     CDS             443..1804
                     /gene="trpE"
                     /locus_tag="ACKB38_001368"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGL7980127.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKSHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVINPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1801..2367
                     /locus_tag="ACKB38_001369"
     CDS             1801..2367
                     /locus_tag="ACKB38_001369"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGL7980128.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGHAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            2378..3382
                     /gene="trpD"
                     /locus_tag="ACKB38_001370"
     CDS             2378..3382
                     /gene="trpD"
                     /locus_tag="ACKB38_001370"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGL7980129.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3379..4146
                     /gene="trpC"
                     /locus_tag="ACKB38_001371"
     CDS             3379..4146
                     /gene="trpC"
                     /locus_tag="ACKB38_001371"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGL7980130.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4133..4732
                     /locus_tag="ACKB38_001372"
     CDS             4133..4732
                     /locus_tag="ACKB38_001372"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGL7980131.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4710..5933
                     /gene="trpB"
                     /locus_tag="ACKB38_001373"
     CDS             4710..5933
                     /gene="trpB"
                     /locus_tag="ACKB38_001373"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGL7980132.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5926..6702
                     /gene="trpA"
                     /locus_tag="ACKB38_001374"
     CDS             5926..6702
                     /gene="trpA"
                     /locus_tag="ACKB38_001374"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGL7980133.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6674..6775)
                     /locus_tag="ACKB38_001375"
                     /pseudo
     CDS             complement(6674..6775)
                     /locus_tag="ACKB38_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7004..7735
                     /locus_tag="ACKB38_001376"
     CDS             7004..7735
                     /locus_tag="ACKB38_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980134.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            7750..8901
                     /locus_tag="ACKB38_001377"
     CDS             7750..8901
                     /locus_tag="ACKB38_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980135.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(8953..9612)
                     /locus_tag="ACKB38_001378"
     CDS             complement(8953..9612)
                     /locus_tag="ACKB38_001378"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGL7980136.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELSLGLLFLLYSWGWLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFQLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9682..10128
                     /locus_tag="ACKB38_001379"
     CDS             9682..10128
                     /locus_tag="ACKB38_001379"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980137.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSKALRKFVSLAFE
                     NRDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            10306..10545
                     /locus_tag="ACKB38_001380"
     CDS             10306..10545
                     /locus_tag="ACKB38_001380"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980138.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFVLMANNLLKYNKRKRQN"
     gene            complement(10655..10858)
                     /locus_tag="ACKB38_001381"
     CDS             complement(10655..10858)
                     /locus_tag="ACKB38_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGL7980139.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(10889..11497)
                     /locus_tag="ACKB38_001382"
     CDS             complement(10889..11497)
                     /locus_tag="ACKB38_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGL7980140.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(11536..11706)
                     /locus_tag="ACKB38_001383"
     CDS             complement(11536..11706)
                     /locus_tag="ACKB38_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGL7980141.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(11718..12284)
                     /gene="amaP"
                     /locus_tag="ACKB38_001384"
     CDS             complement(11718..12284)
                     /gene="amaP"
                     /locus_tag="ACKB38_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGL7980142.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(12368..12532)
                     /locus_tag="ACKB38_001385"
     CDS             complement(12368..12532)
                     /locus_tag="ACKB38_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGL7980143.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPAIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            12975..14456
                     /gene="mgaSpn"
                     /locus_tag="ACKB38_001386"
                     /gene_synonym="mgrA"
     CDS             12975..14456
                     /gene="mgaSpn"
                     /locus_tag="ACKB38_001386"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGL7980144.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            14818..15819
                     /locus_tag="ACKB38_001387"
     CDS             14818..15819
                     /locus_tag="ACKB38_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7980145.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            15970..16887
                     /locus_tag="ACKB38_001388"
     CDS             15970..16887
                     /locus_tag="ACKB38_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980146.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            16898..17788
                     /locus_tag="ACKB38_001389"
     CDS             16898..17788
                     /locus_tag="ACKB38_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7980147.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            17817..19433
                     /locus_tag="ACKB38_001390"
     CDS             17817..19433
                     /locus_tag="ACKB38_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7980148.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNVNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            19443..20762
                     /locus_tag="ACKB38_001391"
     CDS             19443..20762
                     /locus_tag="ACKB38_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGL7980149.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKNLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMVFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            21160..21573
                     /locus_tag="ACKB38_001392"
     CDS             21160..21573
                     /locus_tag="ACKB38_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980150.1"
                     /translation="MKNNERLGIKLSRDSVLGLREVRRLYLGSSDIPVSDGYVIEVAY
                     NQISHEIDIIDWVELNKSKIKISEISESVDIDATSLRTTLTLDTLVYEGMRDIQLKLR
                     ELTKGRVFFSFVVKLVLFASILKKKDLLEKFQEKC"
BASE COUNT         7025 a         3733 c         4491 g         6550 t
ORIGIN      
        1 tacctacggc aaggcgacgt tgacgcggtt tgaagagatt ttcgaagagt ataaaggtgt
       61 accgagatga acatctcttc atcaatgtct ctggtcgtgg aggcaaggac ctagctgcga
      121 ttgcagacta cctagaagct aaaaaataat agatggaaaa attacagttc tttctctcac
      181 agagagcttg tggttgctgg aaacaagcag agaaagctgt aaagttgggt tcatctgaaa
      241 cgagagaaga aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag
      301 atatctgaga caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt
      361 tcgtcctcac gcaagttatt ttgcgtgggg atttttcata cagtcgctac gaactagaat
      421 tagaactgaa agggaaatta caatggaacg aatcattcat ggagatgtct tatcaccaat
      481 cttggcttat atgcgcttaa aggggcaaca caaggttatc ttagagagta ttccgagaga
      541 caaggaaaca gctcgttttt ctatcctagc ctataatcct gtttttgaga ttaagtttga
      601 aaatggagtt ctttatcaaa atggtcaagt gattgatcgg gatcccttgg atttccttta
      661 tgaagtgatt cataagagtc agcaccattc agagctacct tttggtggtg gagccattgg
      721 ttttgtgggt tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac
      781 aattgggacg ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa
      841 gaaggagaag atacatgtca tagaggatgc actttatagt gagcgcagtc aagaagcctt
      901 ggaaaaatcc ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga
      961 agacttggat ctatctccgt tagacttcaa atcccatatc gctcctcaga agtttgagga
     1021 aatggtggaa accgctcgcg acttgattcg taatggcgat atgttccaat gtgtactcag
     1081 ccagcgtttc tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt
     1141 gatcaatcca tccaattacc tctatttcta tgattttggg gattatcaaa tcatcggagc
     1201 cagtccagaa agtttggttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg
     1261 gacgcgacca agaggggcta cggatgaaga agacaaggcc ttggcgactg acctcctctc
     1321 tgatgagaag gaaacagcag aacatcgaat gttggtagac ctgggtcgta atgatattgg
     1381 tcgcatctct gaaacgacca gtgtccaagt cactaagtat atggaagtgg agctcttccg
     1441 ttatgttatg catctgacca gcgtggtcaa ggggcgtttg cttgcagaac tcactgccat
     1501 ggatgccttg aaagctacac ttcctgctgg aaccgtttct ggagcaccaa agattcgagc
     1561 gatgagacgt atctatgaac tggaaacgga aaaacgggga gtatacgctg gggcaatcgg
     1621 ctacttgtct gcgacgggtg atatggattt ggccatcgcc atccgaacca tgattctcaa
     1681 aaatcaaaga gcctatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa
     1741 cgaataccaa gaaaccatta acaaagcaaa atctatgact agaattggag aactaagacc
     1801 atgattttat tgattgacaa ctatgattct tttacctata acttggccca atacattggg
     1861 aattttgcag aagttcaggt tctgagaaat gatgattcca agctgtatga agaagctgaa
     1921 aaagcagatg gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg
     1981 gaagacatga tttgtgattt tgcaggcaag aagccgattc tagggatttg tttgggtcac
     2041 caagccattg cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg
     2101 aaacagagca atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggatgga
     2161 catgcggtca tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg
     2221 acagctcgtt cgactgatga ccaagccatc atggggattc aacataaaaa cctaccgatt
     2281 tatggcttcc agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg
     2341 aattttatcg aggaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact
     2401 agcaaaattt gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac
     2461 tgggcgtgta actgaagcgc agattgcttc tctcctctta gctcttaaga tgaaggggga
     2521 aacacctgaa gaacgcacag ccattgccca agtcatgaga ggacatgccc agcatattcc
     2581 aactgagatt catgatgcca tggacaactg tggtacaggt ggggacaagt ctttcagttt
     2641 taatatttcc acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaaaca
     2701 cggtaaccgc tcgatatctt ctaaatctgg ttccgcagat gtcctcgaag ccttgggaat
     2761 caatcttgac ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt
     2821 tctcttcgct aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt
     2881 gggaattcca acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac
     2941 acagcttctt ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa
     3001 tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt
     3061 gaacggaaca accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac
     3121 tccagaggat ttgggaatgg aaggctatgc tatggaagat attcgtggtg ggaatgctca
     3181 ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac
     3241 gacagtcttg aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga
     3301 aggggttgcc ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact
     3361 gttacaggag taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggcg
     3421 cgtgaggtgg agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg
     3481 gcagaatttt tgaagaatca tcaggaccgc ttgcaggtaa tcgctgaggt caagaaagct
     3541 agccctagtt tgggagatat caatctcgat gtggatattg tgcaacaggc ccagacttat
     3601 gaagaaaacg gagcagtgat gatttcggtg ttgacagatg aggttttctt taaagggcat
     3661 ttggattatc tacgggaaat ttccagtcag gtagagattc cgacgctcaa caaagacttt
     3721 atcatcgatg aaaagcaaat catccgcgct cgcaatgcag gtgcgacagt tatcttgctc
     3781 attgtggcag ccttgtccga agaacgcctc aaggaactgt atgactacgc gacagagctt
     3841 ggtctggaag tcttagtgga gactcacaat ctagctgaac tagaggtagc ccacagactt
     3901 ggtgctgaga ttatcggggt caacaaccgc aacttgacta cctttgaggt cgacttgcag
     3961 accagtgtag atttagcccc ttactttgag gaaggtcgct attacatttc tgaatctgcc
     4021 attttcacag ggcaggatgc ggaacgacta gccccatact ttaacggaat tttggtaggg
     4081 acagctctta tgcaggcaga aaatgtggcc cagagaatca aggagttgca gattgacaaa
     4141 ggttaaaatt tgtggactat cgaccaaaga agcggtggaa acagccgttt cagcaggagc
     4201 cgactatatc ggttttgtct ttgcacctag taaaagacag gtgactttag aagaggcagc
     4261 tgagttggca aagcttattc ctgcagatgt gaaaaaggtt ggagtatttg tttcaccaag
     4321 tcgggtagaa ctgctggaag cgattgacaa agttggcttg gacttggttc aagttcacgg
     4381 tcaggtggca gatgatttat ttgagaattt gccttgtgcc agtattcagg ctgtgcaggt
     4441 agatggaaat gggcatgtcc ccaattctca ggcagattat ctactctttg atgcccctgt
     4501 ggcaggaagt ggccagccct ttgattgggg tcaactggat acgactggac tagcacagcc
     4561 cttctttatc gcaggtggcc ttaatgaaga taatgtagta aaagcaattc aacactttac
     4621 tccctatgca gtagatgtat cgagcggagt ggagacagat ggacaaaaag atcatgaaaa
     4681 gattagaaga tttatagaga gggtaaagca tggcatatca ggaaccaaat aaagatggat
     4741 tttacggaaa attcggcgga cgttttgtcc cagaaacatt gatgacagca gttttggagt
     4801 tggagaaggc ctaccgtgaa agtcaggcag acccaagttt ccaagaggaa ttaaaccagc
     4861 ttttgcgcca gtacgtagga cgtgaaactc ctctttatta cgcaaaaaac ttgacccagc
     4921 atattggcgg agccaagatt tatctcaaac gggaagacct taaccataca ggagcccaca
     4981 agattaacaa tgccttagga caagtttggc ttgccaaacg catgggtaaa aagaaaatta
     5041 tcgcagaaac gggtgctggt cagcacggtg tggcaactgc aactgctgca gccctcttta
     5101 acatggaatg taccatctac atgggtgagg aagatgtcaa acgccaagcc ctcaatgtct
     5161 tccgcatgga gcttttggga gccaaggtcg aggctgtaac agatggttcg cgcgtgctca
     5221 aggatgcggt caatgcagcc cttcgttcat gggtggctaa tatcgacgat acccactata
     5281 tccttggttc tgccttgggg cctcatccat ttccagaaat cgttcgtgac ttccaaagtg
     5341 tcatcggtcg agaggctaaa caacagtacc gtgacttgac aggtcgagat ctgccagatg
     5401 ccctagtagc ctgtgttggt ggtgggtcga atgctatcgg gctcttccat ccctttgtag
     5461 aagatgagtc agtagctatg tatggaactg aagcagcagg gcttggtgtg gatacagagc
     5521 accacgcagc taccttgacc aagggtcgtc caggtgtcct tcacggttcc ctcatggatg
     5581 tgctccaaga tgcccatggt caaattcttg aagccttctc tatctcagca ggtttggact
     5641 atcctggtat cggtccagag cattctcact accacgatat taaacgtgcc agctatgtcc
     5701 ctgtgacaga cgaagaagcc ttggaaggat tccaactctt gtctcgtgtg gaagggatta
     5761 tcccagcctt ggaatctagc catgctatcg cctttgcggt gaaattggcc aaagaacttg
     5821 gaccagaaaa gtctatgatt gtctgcctat caggtcgtgg ggacaaggat gtggttcaag
     5881 tcaaggaccg cttggaagca gatgcagcaa agaagggaga agctcatgcc taagacacta
     5941 acagaaaaat taaatgctat taaagcagct ggaaaaggaa tttttgttcc ctatatcatg
     6001 gctggtgacc acgagaaagg tttggacggt cttgctgaaa caattcactt tttagaagat
     6061 ttgggtgtct ctgcaattga agtgggtatt cccttttcag accctgttgc agatggacct
     6121 gttatcgaag aagctggctt gcgcagttta gcccacggga cctctaccca ggctttagtt
     6181 gaaaccttga aaaccattga aacagagatt ccactggtca tcatgaccta cttcaacccc
     6241 ctctttcagt acggtgtgga gaactttgtc aaagatctgg ctgatacagc tgtgaagggc
     6301 ttgattatcc cagacctgcc tcatgaacat gccaactttg tagaaccatt tttggcaaat
     6361 acagatatcg ccttgattcc tttagtaagc ttgaccacag gaattgagcg ccagaaagag
     6421 ttgattgaag gggcggaggg attcatctat gccgttgcca tcaatggagt gacagggaaa
     6481 tcaggcaatt accgtgcaga tttggacaag cacttggcgc aacttcatca agtggccgac
     6541 atcccagtct tgacaggttt tggtgtatct agtcaagccg atctggaacg cttcaatgcg
     6601 gtgtcagatg gtgttatcgt cggttcgaaa atagtaaaag ctctccacca aggagagccg
     6661 attcaggatt ttatcagaca agcagtagct taccaaaaat aatcaaacaa gtagcaagta
     6721 agaggaaagg cacgaaagga agtctttcct ttttcttttg caggagaaag gtcagtttta
     6781 tagtgaattg aatctggaat attacactac ggttcctaaa atattttcag aaagcgattt
     6841 gaatttccca ataaatttgt tcagttttta attgatttta ctatagaaga tataattttc
     6901 taaattactc tgacacagaa ctttaagaaa aagataatta ttgtagtaat ttctttaaga
     6961 aaatggtata attcattaaa aagtatttgg aggaggaaga attatgttga atcaggatct
     7021 ctttgattcg cttgaggcac aaaaaattgt agatactttg atgaaagggc aaaaagatta
     7081 tgtagatgaa cgtctagaaa aaagagagac aatgatagtg tcgaatggtt atgcatggac
     7141 gcgacctaat catattgata ctgcatttgc atcagcagat ttgtttgagt ataaattaca
     7201 attagcagga cagacttggg gatatttaga atttgaaaca aatacagaaa aatatgggaa
     7261 agtattgtta attataaagg gtaagaagcg acttacaaac caatttcctt tggtacaaaa
     7321 aaataagagt ggctacttat ttgaatatgc tcagatgaat acactttatc ttaatcaaca
     7381 ttcttcctac aaaaatgatg aaaatagtca ttcctttcca attcagatgg agttagtttc
     7441 tgatgaaatg attcaagaaa ttgaacaagc tactaaaaat tcgaatatcg aaaaatttat
     7501 gattttaact tatgaggcgg actcagaaaa caatattata tctgtagatg ttgttatgcc
     7561 tgatgcacga actggtcagt tacacttgat tcaagatttg tctgagtata ttcaatcaag
     7621 ttcgtaccat ttcgaagaag ctaaatatca agatattcct aatttttcag aattatcgga
     7681 aacagaagat tttgaaatta ttccaagaat agaaaaacaa gaaggtcaaa agtagtaagg
     7741 agtatgtaaa tgtttaatgg tcgggtattg aaagaattac ggctgttaaa tggtttaagt
     7801 agagcagaat tagctcagag aattaattta acggaacaag ccatttggca gtttgagtcc
     7861 aacgaaacga aacctaaatt atcaaccaaa atgcatttgg ccaaccaatt tcatgttgat
     7921 ttaacttatt ttgaacagga agaagagagc attcgatttg attcttctgt aattgccttt
     7981 agaaatgcag acctagcaac acggaaaaca atagatattc aaactatgta tttacataag
     8041 gtagatagtt tgattgatta ttttgaaagt tttgtaatta tacctaatat tataattcat
     8101 gacctaagta atgtagtgag tgaatcttat cataagggag aatccattga ggaattggct
     8161 ctttatgcca gggaaaaatt aggtatttca aaagataatc atgatttgct ttataaatta
     8221 gaacgttcag gcatctatat cgtggaacga ttaattaatg gccaagctga tgcttatagc
     8281 gcatggtcaa aattgggaag accttatatt gtgttaggaa cgaataaatc atctgtacgt
     8341 cgaaattttg acttagctca tgagctagga catattcttt tacataaata taaagatatg
     8401 aatgaagatg gcgatcgttt ggagcaagaa gcaaattatt ttgcatcatg ttttttattg
     8461 ccaaaagaag agtttttagt caaatttgaa gagagggttg gcaagcgtgt cagcaatcct
     8521 gatagttata ttttattgaa gtcggatttg aatgtttcga tacaggcttt agagtatcga
     8581 gcttttaagt taggattatt gactccaaag caacattctt acttttatcg tcaaattgcg
     8641 caaaaaggtt acaaaatgat tgaacccttg gatgatcaaa tttttgttaa aaaaccaagc
     8701 aaagtaaaga gtattctgga cgtcgttttg agtaatcatc tagtcagtct agcgactata
     8761 atgtctaaac aaagtattcg tttacagttt ataagcgaaa tattttcagt cgaaatgaaa
     8821 ttttttgatc agtatcaaga agatagaaga acagatcgat ttgataacat catccctttg
     8881 tacaaaagaa ataatttata atttaactgt gaaataattt catgaaggat atatggcaga
     8941 aattggattt tatcagacaa gcagtagctt accaaaaata atcaaacaag tagcaagtaa
     9001 gaggaaaggc acgaaaggaa gtctttcctt tttcttttgc aggagaaagg ccaggatacc
     9061 cgtcgcagaa gcgaactgaa tcaagatcag taactccgtt acgctaaaga cgagagcaca
     9121 agaagctaaa aagaggaaat cccctgcacc catgcggata tcgataaaat gagccaaaat
     9181 tccaagtatg aggaaggaga ccatgaccag attccagcca gaggaagcta ttaggattag
     9241 ctggaaagtc atccagacca gtaagggata ttcctgatgg tgaaagtcgt agatacccaa
     9301 ggtcaaacca gcggtgatta ggacgacttg ccccaaggag agccatcccc aagagtaaag
     9361 cagaaagagg agtcctaagc ttaattcaaa gagggcatac cagacaggat agcgaacttt
     9421 gcagtagcga cagcgaaagc gattgaagac ctgtgagaga atcggaatca aatctaaggg
     9481 acgcaaggga gtctgacagg aatcgcagtg actggctgaa ctgataatgg attgctctgg
     9541 aaaacggtca atgaccaaac caagaaagga agctagaatg ctcccgacga gaaaaaaata
     9601 aaaatcaatc atacttatct attcgtaaaa aatgggagaa atagtataat ggagaaacat
     9661 agataaggtg gtgaggaaaa gatgacaatt cgttttgaag aaaaggtgag cacagaaaac
     9721 gctcaatttg tatgccagtg gtccaactcc ctaggcaaat cctttcaaga acaatggatg
     9781 ggaccaagga ttccttttct actgacactt caagccttgg aaggagtctt ttcaatcttt
     9841 gatgaacaag agtttgtggg atttatccag aaaatcaggc tagaagacag caatcttcat
     9901 atcgggagat tttttatcaa cccccagaaa caggagcaag gcttaggtag caaggcttta
     9961 aggaaatttg ttagtttagc atttgaaaat agagatatag acagtatttc tctaaatgtc
    10021 ttcgaggcaa atcaaagagc tcagaatctt taccaaaaag aaggatttga aatcgttcaa
    10081 atggttgaag cacctgtacg aaaatatagt agattgaaac tagaatagta cacctctact
    10141 tctaaaacat tgttagaaat cgatttgact gtcccgatca attcgtcctg ttattatttc
    10201 attttattat acattatgaa aaaggggaga tagaaagcaa acagccttga aatcaatgtt
    10261 gactgcgatt atgaacctga atcagcccct caaaagagac tgtatatgaa tgaacgttac
    10321 cagtgtttaa aaactaaaga atatcaggca cttttatctt ccaagggtag acaaattttc
    10381 gctaaacgta agattgatat gaaatctgtc tttgggcaga taaaggtttg tttgggttat
    10441 aagagatgtc atctgagagg taagcgtcaa gtgagaattg acatgggatt cgtactcatg
    10501 gccaacaacc tgctgaaata taataagaga aagaggcaaa attaaaaagc tagagttccg
    10561 caattgggaa tctctagctt tttgttttct gagaatcatc ttgactcaga ctcttttgac
    10621 taatattact aaaaagataa agtaactttt aaccttattt atcgtcgcca ctcaacatgt
    10681 ttttaacacc ttctacagca ccttctacag cgtctttagc gtcttcggca acttctttta
    10741 ctttagaaac aactttttca gctgcacctt ctttttccat tttttcatca ccgatggctt
    10801 tcccaacacc ttctttaata gaacctttag cttgatttaa tttttcttct actgacatga
    10861 taatttctcc tgttatattt attttatctt agtttacacg agtgttttcg tgagatacta
    10921 caccatttgc tgcaccttta acagcttcta caccttcgct aactttttct tgaacagttg
    10981 agaaaccaga tccaagacca gatttagctt tttcgaattg ttctgaagtg aattctcctg
    11041 ttgattcagc aacgtcagat acgcgatctt gaaggcttac tgagtctgct tcatgctgtt
    11101 ctttagtttt gatgtcgaca acgtttacgt tgatttcaac aatttccaag tcagtcattt
    11161 tagcaacttc tgaagatacg atttctctga tttctgaata taaagctgga acattttttt
    11221 ggtactcaac aataacgttt aagtcaactg caacttgtgt tttaccaact tctacgttaa
    11281 caccacttgt tacgtcatcg ctgttaacga ttttttcttt aagatttgag aagaaaccac
    11341 catcgattcc caaaagacct gaaacgtttt ctagtgaaag accaatgatt ttttggataa
    11401 ctttatcttc gtaagtaagt tcccctttga tttcgtgagc tacaacagta gcatctttct
    11461 tttctacgtt agtgtttgtg tttttttcgt ttgacatatg aaactccttt aattaaatta
    11521 gtaattttta tttttttatt tatctatata gtttttttcg atatataatc cagctgcaac
    11581 tcccagtgct cctaaaatca atacaaatat tgttttgaag aagccaaagg agacaatcaa
    11641 acaagccaga aatacgccta tgagaccagc gataattgga tatcgatatt gtttaagcca
    11701 ttccattttt tacttcctta cttcacacga ctaacagtct ttttgttttg aggttttggt
    11761 tggtaatttt ttactgcaac ttcaagtttt acttgacgct caataccaaa aaactgcttc
    11821 aatccattag ttatttcatt ttgaattatt tggcatctgt cagcgatgtt gtctgaagga
    11881 agaattttac cttctacatg aacgaaacat ttattttttc gtaaatttac atgaacagtt
    11941 gggttcttga tcaatctatg atcactcacc aaacttctaa caaaaccttc gattgccgaa
    12001 ttctttaatt ttaatgtatc gtttttagtt tcaagttgga tttccaagta acgtttagga
    12061 taaaacatca caactaaaat ggataataaa actaaagttg atagaaccac tgtcccccaa
    12121 aagacatatc tagcaagata gaattcaact acggagttct gtctccagct aagtagatga
    12181 atacctagat cactaacttg atgataatct atcaaaacag gaaggaaaat tgtcaagatt
    12241 aaaatacaga aaataatgaa acatattttc tttgcttttg acatatacaa tcctttcgat
    12301 ttaacattta aactattttt cccgtcattg atttaaaaat agtttgctaa ctttactggc
    12361 gaaaagttta ctttctacct gtaaagaatg acactacagt aatcacaatc gctgcaccta
    12421 caatagatgg gagcaaagcc attccagcga ttgctggacc ccaactacct aaaagagatt
    12481 gtcctgcata tgccccgact aaaccagcga atacatttgc gatgattccc atagaactac
    12541 ttttttagtg attttacctg ccaagagacc aataagacct ccagcaataa ttgatcacaa
    12601 cactgcctac cctccttttt tattttaata tcaaaaaatc aatttctttg attattacta
    12661 ttatataact ttaatataga aaagtcaatt atttcgattg ttattattat ataattttaa
    12721 aaaatcaaat tctttaattg ttgctattat ataattttta aaattaaaaa tataatcttt
    12781 agacttgaat ttgattctgt attcacgccc tcaatagacg gtaaaataag aaaattgttt
    12841 atatattgtc tccgtagtgt tattatacga aataaaagat tttaggaaaa gtcgttatat
    12901 catgctatac ctattctttg tggtataatt gcaagaggtt taatccgata atttataaag
    12961 agagaaagat attcatgaga gatttattat ctaaaaaaag tcataggcaa ttagaattat
    13021 tagaattatt atttgaacat aaacgttggt ttcatcgttc tgaactagca gagttattaa
    13081 attgtacaga acgtgcagtc aaagatgatc tatcccatgt taaatctgct tttcctgact
    13141 tgatttttca ttcttctact aatggtatac gcattattaa taccgatgat agtgatattg
    13201 aaatggttta ccatcatttc tttaaacatt caactcattt ttcgatttta gaattcatct
    13261 tctttaatga aggttgtcaa gctgagagta tttgtaaaga attttatatc agttcatctt
    13321 cgctctatcg tattattagc caaatcaata aagtgattaa aaggcaattt caatttgaag
    13381 ttagtctgac ccctgttcaa atcattggaa atgagagaga cattcgttac ttttttgcac
    13441 aatatttttc agaaaaatat tatttcctag aatggccatt tgaaaatttt tcatcagagc
    13501 cactatctca attgttagaa ttggtttata aggaaacaag ctttccaatg aatttgtcaa
    13561 cgcatagaat gctaaagttg ctcctagtta cgaacctata tagaataaag tttggtcatt
    13621 ttatggaagt agataaagat tcttttaacg accaaagttt ggatttttta atgcaggcag
    13681 aaggaataga aggtgttgct cagagttttg aatcagaata caatatctct ttagatgaag
    13741 aagttgtttg ccaattattt gtgtcttact ttcaaaaaat gtttttcata gatgaaagtc
    13801 tctttatgaa atgcgtaaaa aaggatagtt atgttgaaaa atcttaccat ctattgagtg
    13861 attttattga tcagatttca gtcaagtatc agattgaaat ggagaataag gataatctga
    13921 tttggcatct gcataatacc gcacatctgt atcgtcaaga gttgtttact gagtttattt
    13981 tatttgatca aaaagggaat acaatcagga actttcaaaa tatttttcca aaatttgttt
    14041 cagatataaa aaaagagctt tctcactatt tagagactct tgaggtttgt tctagttcaa
    14101 tgatggtaaa tcatctatct tataccttca tcactcatac caaacatttg gtgattaatt
    14161 tgctacaaaa tcagcctaaa ctaaaggttt tagttatgag taattttgat cagtatcatg
    14221 caaaattcgt tgcagagaca ctttcttatt attgtagcaa taattttgaa ctggaagttt
    14281 ggactgaatt agaattatca aaggaatctt tagaagattc accttatgat atcattattt
    14341 ctaattttat tattcctcct attgaaaata agagactcat ctattcaaat aatataaaca
    14401 cggtctcact catatacttg ttaaatgcca tgatgtttat tcgattagat gagtaaccaa
    14461 aaataacaaa aaaacgctat ttcgcgtttg aactgtacct caaaagttag acagaaaaag
    14521 tctaattttg ggggtgtttt tgttaagaaa agaccttcaa tcatttaagc aaaatgtaca
    14581 tctagattta tagccatcta aaactcttgc tttttttata gaagatacga aaaaagccat
    14641 gaaaccaagg cttttcctgt tttatgtaga tgtttacaca tgaaaattga agtattttca
    14701 aaagctaatt ggagtgattt tattcatgat gggataaatg atctggccaa aaatgattga
    14761 gaaatagttg cttaaaaagc atttgcatga tactatatat tgaaaggagg tgataatatg
    14821 gtagaacaaa gaaaatcaat taccatgaaa gatgttgctt tagaagcagg agttagtgtt
    14881 ggaactgttt cacgtgtaat taataaagaa aaaggcatta aagaagtaac tttgaaaaaa
    14941 gtggaacaag cgattaaaac tttgaattac attccagatt actacgctag aggaatgaaa
    15001 aaaaatcgaa cagaaacgat tgcaatcatt gtaccaagta tctggcatcc cttcttttca
    15061 gaatttgcta tgcatgtgga aaatgaagtc tataagagaa ataacaaatt actcttatgt
    15121 tctatcaatg gtacaaatag agagcaagac tatctggaga tgttgcgtca taataaagtt
    15181 gatggagtgg ttgccattac ctatagtcca attgaacatt acttgacgtc aggaattccc
    15241 tttgttagta ttgaccgcac atactcagat attgccattc cttgtgtttc atcagataat
    15301 gatgcaggtg gaagagaagc agctaaacaa ttaattagta aaggatgtca gcatttagcc
    15361 tttgtgggcg gacataatac aaccattaat gaaaccaaac gtcgtagaat ttcatttgaa
    15421 aagtatgtcc aagagcataa aatacctctt agtatttttg atttggatga gacagttact
    15481 gactattatg gaaagctaga gagtttcttg gaagaaaatt cttctataga tggaattttt
    15541 acaatcaacg attttacagc tttggatgtg attgaaatac tagaaaaaaa tggcaaacgt
    15601 attcctcatg atgtccaaat tattggatac gatggaatta aaatggctgg agatagagat
    15661 tatctacttt caactattga acaaccattg gaagaaatgg caaaagaagc ggttcgtatt
    15721 ttgtttgata taattgatgg aaagactgtt aatttgcaga caatcctacc agtaaaattt
    15781 gttgaaggaa aaacaacaaa aaatgaaaat aagtcttgac agaaagcgct atcaatgata
    15841 gaatgaattc agataaaaag atttattttt aaaacaaaaa tgaaacgttt caaaaaaaga
    15901 aataaagaga cagcgccaag cgctatcttt tctagaaaaa aatgaaacgt ttcaaaaaag
    15961 gaggttgcta tgaatagcaa agcgaagcaa gtttctcttt gggaaagaat caagaaacaa
    16021 aaactcttgt tattgatgac tgtccccggt ttagttttaa cctttatctt taaatacatc
    16081 cctatgtatg gggttttaat cgcatttaaa gattacaatc ctttaaaagg aattttaggg
    16141 agtgattgga ttggtttttc tgagtttaca aaattcatat cctctcccaa ctttggtatc
    16201 ttgttagcca acacattaaa attaagtatc tatggtttat tgcttggctt tttaccacca
    16261 atcattctcg cgattatgct caatcaactc ttgagtgaaa aagtcaaaaa acgaattcag
    16321 ctcattttat acgcaccaaa ctttatctca gtcgttgtta ttgtcggtat gattttcctc
    16381 ttcttttcag tgggaggacc aatcaacaat tttctttcta tgtttggaat gaaggctgac
    16441 ttcttgacaa atccagactt ctttagacct ttatacatct ttagtggtat ctggcaagga
    16501 atgggctggg cttcaacgct ctacacggca acattggtaa atgtagatcc agccttagta
    16561 gaagcagccc gactggatgg agccaatatc ttccaacgaa tctggcacat tgatattcca
    16621 gctcttaagc ctattatggt tatccaattt gttttagctg caggtggaat tatgaatgtc
    16681 ggatatgaaa aagcattctt gatgcagaca tcgttaaatt tgccaacttc tgaaattatc
    16741 tcgacatatg tctataaagt tggtcttgta tcaggagact attcttactc aacagcggtt
    16801 ggtttgttta atgcagtgat taacgtagta ttgcttgttg cagttaacca aatcgttaaa
    16861 cgcatgaata atggtgaagg aatttaagga ggaaagtatg aaaaattcga ttatggatac
    16921 aaaatttgat agacgtatct tactcttaaa taaaatcatt attgtcttta tcgttttgat
    16981 gactttgctt cctttacttt atatcgtcgt agcatccttt atggatccta aggttctggt
    17041 tagtagaggg attagcttta atccagccga ttggactgta gaaggttacc agcgtgtatt
    17101 cagtgaccaa tctattctaa gaggttttat caattctcta ctatactctt ttggatttgc
    17161 agctttaaca gtcttgctat ctgtgtttac agcttatcct ctttctaaga aagacttggt
    17221 tggacgtcgt tggattaact acttcttgat tgtaactatg ttctttggtg gtggtttagt
    17281 cccaacttac ttgctcgtaa aagaattggg aatgctcaat actccatggg ctatcattgt
    17341 tccaggtgct gttaacgttt ggaatattat tcttgctagg gcctatttcc aaggattgcc
    17401 tgaagaatta gttgaagctg ctgtcattga tggtgcaaat gatttacaga ttttcttcaa
    17461 aatcatgctt cctcttgcaa aaccaattat gtttgttctc ttcctttatg cttttgtagg
    17521 acagtggaac tcatactttg atgcaatgat ttatatcaag gatccaaact tggaaccatt
    17581 gcaacttgta cttcgtaaaa ttctcattca gagccaacca ggtcaagaca tgattggagc
    17641 acaagcggct atgaatgaaa tgaaacgttt agctgaattg attaaatacg caactattgt
    17701 catttccagc ttgccattga ttgttatgta tccattcttc caaaaatact ttgataaagg
    17761 aattatggct ggttcactta aaggataaaa aaagaaaaaa taaaaggagt tttctcatga
    17821 aattcaaaac attctcaaaa tcagcagttt tgttgacagc tagtttagca gtacttgcag
    17881 cctgtggctc aaaaaataca gcttcaagtc cagattataa gttggaaggt gtaacattcc
    17941 cgcttcaaga aaagaaaaca ttgaagttta tgacagccag ttcaccgtta tctcctaaag
    18001 acccaaatga aaagttaatt ttgcaacgtt tggagaagga aactggcgtt catattgact
    18061 ggaccaacta ccaatccgac tttgcagaaa aacgtaactt ggatatttct agtggtgatt
    18121 taccagatgc tatccacaac gacggagctt cagatgtgga cttgatgaac tgggctaaaa
    18181 aaggtgttat tattccagtt gaagatttga ttgataaata catgccaaat cttaagaaaa
    18241 ttttggatga gaaaccagag tacaaggcct tgatgacagc acctgatggg cacatttact
    18301 catttccatg gattgaagag cttggagatg gtaaagagtc tattcacagt gtcaacgata
    18361 tggcttggat taacaaagat tggcttaaga aacttggtct tgaaatgcca aaaactactg
    18421 atgatttgat taaagtccta gaagctttca aaaacgggga tccaaatgga aatggagagg
    18481 ctgatgaaat tccattttca tttattagtg gtaacggaaa cgaagatttt aaattcctat
    18541 ttgctgcatt tggtataggg gataacgatg atcatttagt agtaggaaat gatggcaaag
    18601 ttgacttcac agcagataac gataactata aagaaggtgt caaatttatc cgtcaattgc
    18661 aagaaaaagg cctgattgat aaagaagctt tcgaacatga ttggaatagt tacattgcta
    18721 aaggtcatga tcagaaattt ggtgtttact ttacatggga taagaataat gttactggaa
    18781 gtaacgaaag ttatgatgtt ttaccagtac ttgctggacc aagtggtcaa aaacacgtag
    18841 ctcgtacaaa cggtatggga tttgcacgtg acaagatggt tattaccagt gtaaacaaaa
    18901 acctagaatt gacagctaaa tggattgatg cacaatacgc tccactccaa tctgtgcaaa
    18961 ataactgggg aacttacgga gatgacaaac aacaaaacat ctttgaattg gatcaagcgt
    19021 caaatagtct aaaacactta ccactaaacg gaactgcacc agcagaactt cgtcaaaaga
    19081 ctgaagtagg aggaccacta gctatcctag attcatacta tggtaaagta acaaccatgc
    19141 ctgatgatgc caaatggcgt ttggatctta tcaaagaata ttatgttcct tacatgagca
    19201 atgtcaataa ctatccaaga gtctttatga cacaggaaga tttggacaag attgcccata
    19261 tcgaagcaga tatgaatgac tatatctacc gtaaacgtgc tgaatggatt gtaaatggca
    19321 atattgatac tgagtgggat gattacaaga aagaacttga aaaatacgga ctttctgatt
    19381 acctcgctat taaacaaaaa tactacgacc aataccaagc aaacaaaaac tagaggttga
    19441 ttatgggaga taagaaatac acagtagaaa aagccaatcg ttttatagca gaaaataaac
    19501 atctcgttaa tactcaatat aagcctgaag aacatttttc agctgagatt ggttggatca
    19561 atgatccaaa tggatttgtc tattttcgtg gagaatacca tctcttttat caattctatc
    19621 catatgatag tgtttggggg cctatgcact ggggacatgc taaaagtaag aacttggtga
    19681 cttgggagca cttgccagtg gcacttgctc ctgaccaaga ttatgaccga aatggttgtt
    19741 tctcaggctc tgccattgtc aaggatgatc gcctctggct catgtacact ggacatatcg
    19801 aagaagaaac cggtgtccgc caagtgcaaa atatggtatt ttcagatgac gggattcact
    19861 ttgaaaagat ttcccaaaat ccagttgcaa ctggatcaga cttaccagat gagttgattg
    19921 ctgctgattt ccgtgatcca aaactctttg aaaaagatgg acgctattac tccgtagtag
    19981 ctgccaaaca caaggataat gtgggctgta tcgttctact agggtccgat aacctagtag
    20041 aatggcagtt cgaatccatc tttttaaaag ggggagaaca ccaaggtttt atgtgggaat
    20101 gcccagatta cttcgagtta gatgggaaag attgccttat tatgtcaccc atgcgttatc
    20161 agcgtgaggg agactcatat cataacatca actcatcgct tttgttcacg ggtaaggtag
    20221 attggagaga aaaacgtttt atcccagaat cagttcaaga aattgatcat ggccaagact
    20281 tctatgcgcc tcaaacattg ttggacgatc aaaatcgtcg tatcctgatt gcttggatgc
    20341 agacatgggg gcgtaccctt ccaacccatg accaagaaca caagtgggca tgtgccatga
    20401 ctctacctag aattctaaga ttggaagatg gcaaactaag acaattccct gttaaaaaag
    20461 gccaatatca aatccaaata gataaagatt gtcattacca cttaggaaat gatatagatt
    20521 atcttgaatt tggttatgac agtaatgcgc agcaagttta cattgatcgt agccatctta
    20581 ttcaaaaaat tctaggtgaa gaagaacagg acactagtcg acggtatgta gatattgaag
    20641 ctaaagaatt ggaagttgtt ctagataaaa attccatcga gatttttgtc aatcaaggtg
    20701 aagcaagctt gactgcaact tattacttaa cggtgccagc tgagctatca cgaattgatt
    20761 aaaaattaag ttatttctcc taaagaaaaa gttctctttc taaaatagtg gaaagaggac
    20821 tttttgtgtt ttgggtatat aagcttagtt tatggtattt gtaaaattgg tgttggatta
    20881 tgatttaagc tagttttcta aagaatttga aaaaaatttt atttaagcaa aaaaaccttg
    20941 gttccaaggc ttttcctgtt gtatttagat gccccctaca gggattgtag gagatatgtt
    21001 gcttagatgt tcttgatttt ctggtgtttt gtaacgttta aatgagtttt ttgagtttgt
    21061 tggtggggcg ttgcccggca attgcccgac ttattgcttg aaaaagaatt taaaatatag
    21121 tatagttaat tatagattaa cacttgcttg gaggaactga tgaagaacaa tgaaagatta
    21181 ggtattaaat taagtagaga tagcgtttta ggattgaggg aagttagaag gctttattta
    21241 ggcagttcag atatcccagt ttctgatggc tatgtgattg aagttgctta taaccagata
    21301 tcacatgaga ttgatattat tgattgggta gagttgaaca agtcaaaaat taagataagt
    21361 gaaattagtg aaagcgtgga tatagatgcc actagcttga gaacaacttt gactttagac
    21421 acattagtat atgaaggtat gagagatata cagttaaagt tgagagagct tacaaagggg
    21481 agagtattct tttcatttgt agtgaagtta gttttgtttg cttctatttt aaagaaaaaa
    21541 gatttactag aaaaatttca agaaaagtgt taatcaagta ttgacacttt atctggattt
    21601 cggtataata tgcttagaaa ggaatctttc taaatttttt tcgtccttat gtgttaatca
    21661 aagacgaata caaaaacata tttttttact ctaaaaagtg ttaatcaatg atgtatttgt
    21721 tagagaggta gataaatgga attgagagca ccaccagtta taatagtata aaacgtataa
    21781 taaaaatatt ttaacttga
//
LOCUS       DBDESG010000039        21532 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.039, whole genome shotgun sequence.
ACCESSION   DBDESG010000039 DBDESG010000000
VERSION     DBDESG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.039"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            527..1990
                     /locus_tag="ACKB38_001393"
     CDS             527..1990
                     /locus_tag="ACKB38_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGL7980151.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            1983..2411
                     /locus_tag="ACKB38_001394"
     CDS             1983..2411
                     /locus_tag="ACKB38_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7980152.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            2389..2838
                     /locus_tag="ACKB38_001395"
     CDS             2389..2838
                     /locus_tag="ACKB38_001395"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HGL7980153.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            2866..3177
                     /locus_tag="ACKB38_001396"
     CDS             2866..3177
                     /locus_tag="ACKB38_001396"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGL7980154.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            3203..4288
                     /locus_tag="ACKB38_001397"
     CDS             3203..4288
                     /locus_tag="ACKB38_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGL7980155.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGILSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHAEPVMTVEEEIDLSDIEIL"
     gene            4299..4994
                     /gene="alsE"
                     /locus_tag="ACKB38_001398"
     CDS             4299..4994
                     /gene="alsE"
                     /locus_tag="ACKB38_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose 6-phosphate 3-epimerase"
                     /protein_id="HGL7980156.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKATIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            5031..6998
                     /gene="tkt"
                     /locus_tag="ACKB38_001399"
     CDS             5031..6998
                     /gene="tkt"
                     /locus_tag="ACKB38_001399"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGL7980157.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRVRYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAVLPTFLGGSADLAHSNMTYIKADGLQD
                     KYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELP
                     VTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTTT
                     PTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELVL
                     QGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIG
                     IDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            7373..8644
                     /locus_tag="ACKB38_001400"
                     /pseudo
     CDS             7373..8644
                     /locus_tag="ACKB38_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8718..9710)
                     /locus_tag="ACKB38_001401"
     CDS             complement(8718..9710)
                     /locus_tag="ACKB38_001401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HGL7980158.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYYLSDKMPAQGWKIHISSQIK
                     DAVNIFKIVYKLSQLNNCSFKVVKNLEELKKINSPREMSPTANKFITLYPKSESEAKS
                     MICNLTNRLSEFKAPKILSDYQCGMHSPVHYRYGAFLKKQAYDEKNKKVIYLLLDEKR
                     KNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVSSKDSAINKYKMEKIIKRSNK
                     GNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDIKNEAHMLKKLADKSYTTNLT
                     DEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKTLDNIVNIVSYIHKLGI"
     gene            10259..10936
                     /locus_tag="ACKB38_001402"
     CDS             10259..10936
                     /locus_tag="ACKB38_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGL7980159.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            10923..11720
                     /locus_tag="ACKB38_001403"
     CDS             10923..11720
                     /locus_tag="ACKB38_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGL7980160.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVIFVEKIAALLSQWKEDKGWSIDILPSQASTNPFHHI"
     gene            11737..12840
                     /locus_tag="ACKB38_001404"
     CDS             11737..12840
                     /locus_tag="ACKB38_001404"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGL7980161.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEVSHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            13141..14160
                     /gene="galE"
                     /locus_tag="ACKB38_001405"
     CDS             13141..14160
                     /gene="galE"
                     /locus_tag="ACKB38_001405"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGL7980162.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVEGITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            14162..15127
                     /locus_tag="ACKB38_001406"
     CDS             14162..15127
                     /locus_tag="ACKB38_001406"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7980163.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(15178..15384)
                     /locus_tag="ACKB38_001407"
     CDS             complement(15178..15384)
                     /locus_tag="ACKB38_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGL7980164.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            15436..15912
                     /locus_tag="ACKB38_001408"
     CDS             15436..15912
                     /locus_tag="ACKB38_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGL7980165.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            15921..16592
                     /gene="cmk"
                     /locus_tag="ACKB38_001409"
     CDS             15921..16592
                     /gene="cmk"
                     /locus_tag="ACKB38_001409"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGL7980166.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPQVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            16723..17061
                     /locus_tag="ACKB38_001410"
     CDS             16723..17061
                     /locus_tag="ACKB38_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGL7980167.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            17064..17693
                     /locus_tag="ACKB38_001411"
     CDS             17064..17693
                     /locus_tag="ACKB38_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGL7980168.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            17933..19084
                     /locus_tag="ACKB38_001412"
     CDS             17933..19084
                     /locus_tag="ACKB38_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGL7980169.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            19402..20151
                     /gene="truA"
                     /locus_tag="ACKB38_001413"
     CDS             19402..20151
                     /gene="truA"
                     /locus_tag="ACKB38_001413"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGL7980170.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            20141..20914
                     /locus_tag="ACKB38_001414"
     CDS             20141..20914
                     /locus_tag="ACKB38_001414"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGL7980171.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            20892..21353
                     /locus_tag="ACKB38_001415"
     CDS             20892..21353
                     /locus_tag="ACKB38_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGL7980172.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSALMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         6346 a         3830 c         4731 g         6625 t
ORIGIN      
        1 aaagactgca atctgtggat tgcagttttt tatacgatgg atctatcgta gatctgatgt
       61 gcaaggccta cgtgccgatc atctatcggt gaacccaaga gcgaccctca agcctgcttg
      121 gattgaggta atagattcaa atatctgtag ttagactatt tgaagtttga tgtaagaaag
      181 agaaagcgac agattgaagt aattttaact ctcttctatt gctagaacaa atggtcggat
      241 aggttggtag tttgaaaatg aagatgctat ctattgttaa atggaacata gtgttattta
      301 ttagaaaatc gtttggttta tttcttatca aatacgaaaa gcaacttaaa tatttcaact
      361 aaaatagatg ttatgaagaa aaggtaaaat gattttggca tagtgaggtt ctgttctatt
      421 tgatatcata tttttgataa aaacaaaaat gtccattgca aaggacaaaa tgcgaagtat
      481 attatttttt gaaagcgata taatggattc ataaaggagg tgtatcgtgt ctagaaaaca
      541 agaacaaatg gaaacgttgt tgctcctttt gcgagatagt aaggattata tatctgctaa
      601 agtattggga gaaaaattaa attgctctga taaaacggtt tatcgccttg tcaagggaat
      661 caacaaagat tgtccggtag aagcattcat tttatctgaa aaaggcagag gtttcaaatt
      721 aaatccaaga agttccctcg tggacgttga tgggaatttt acagaggctt ttgatcctga
      781 agtaaggcgt gaaaaattac tagaacgtct cttgttgact gctcctaagc cacattctat
      841 ttatgattta ggagaggaat tctacgtaag cgagtcagta gtactaaaag atcgtcagat
      901 attacaagag agtctagcaa tttatgggtt agatttaaaa atgagacaac gaaagctttt
      961 tattgatggg gatgaggctc aaattcgttc agccattcta aatctactgc caatgtttaa
     1021 tcagttggat ttagagcaaa ttacacagaa taaggttcag cctcttgacg gagaacttgc
     1081 tcacttttgt ttgggattac tgattacact tgagagagaa ttgggggtaa acattcccta
     1141 tccatataat ataaatattt tctctcacct gtatattttt atcagtagga atcgtcgtag
     1201 tactagtatt catgttgtag caccttcaaa acctactatt gttgatgaga aaatttacag
     1261 tgtctgtcaa aaaattattc aagaaattga acaatatttt aggatgaagg ttgatgcagt
     1321 tgagattgac tatctttatc aatacgttgt atcttcgaga ttgcaaaaac cattttcttc
     1381 cgggaagctt cctttttctc agcgagtttt agatgtcact cattactatt ttagccgtat
     1441 gtgtatggac aatagagaga ttgaaacgac agatcctgac tttgttgact tggcgagtca
     1501 tatcagtccc ttactgagga gattagataa tagagtacag attaagaata gtcttttatc
     1561 acaaattctt ttaacctatc ctaatctggt taaagagtta acaactattt ctaaagaagt
     1621 gagtctagta tttggttttg cttccttgag tctggacgag attggttttc tagtcttata
     1681 ttttgcacgg tttcaagaaa agcgagcacg tcctctaaaa acagtagtga tgtgtacatc
     1741 aggtgtcgga acttcagagc ttttacgagc acgattagaa aagcaatttt ctgaattgga
     1801 tattattgat gtagttgctt atcatcaatt agatgagctg ataaatctat atccagattt
     1861 agatttcatt gtgacgacgg tagctttgca ggaaccagca agtgtcccgt ttgtcctagt
     1921 tagtgttttt ctaaccgagg gtgataaaca acgtcttcaa gcaaaaattc aggagataaa
     1981 ctatgaataa tctttcgctt gtccttatgg atatatctgt tcaaaatcgt caagaagcct
     2041 acaaagaatt agcaaatcaa atcagccttc ttgtttctga agatacagaa aaaatagaag
     2101 agcttctata ttaccgtgag agacagggaa gtatagaggt tgctaaaggt gttcttctac
     2161 cacattgtga aggaaacttt caacatcatg tcttagtgat tactagatta aaatcaccta
     2221 tcagagaatg gtcgaaggat atccagtgtg ttgaccttat tatcggtttg gccattgcag
     2281 tatcacagga caagtcatgt attaaaacat tgatgagaag actagcagat gaatcattca
     2341 taaatcaatt aaaacagtta acaaaagaag aattacggga gataatatat ggaaatcaaa
     2401 gatattctta atgtgagtct gatccagacg gatttacaga tgcagagcaa agaagaggtt
     2461 tttgaggcat tagctcaact attggttgag acgggttatg tgtctgatag agaccaattt
     2521 atcgaaggtc tttatcagag agaggcagaa ggacagaccg gtattgggaa ttatattgct
     2581 attccccata gcaagagttc tgctgtggag aaggcggggg tagtcatagc tataaatcac
     2641 aatgagattc cttgggagac cattgatggg aaaggggtca aagtaattgt actctttgca
     2701 gttggtgatg atacagaagc tgctagggag catttgaaga ccttatcact ctttgctcga
     2761 aaacttggta atgacgaagt tgttgccaaa ttagttcggg ctcagacatc tgatgatgtg
     2821 attgcagctt tttgttaata agaaaaaatt ttggagggta tccgtatgaa aattgttggt
     2881 gttgcagctt gtactgtggg aattgcccac acttatattg cacaggaaaa attagagaat
     2941 gccgcaaagg tagctggaca tgtgattcat gttgagactc aggggacaat aggggtagaa
     3001 aatgaattga gtcaagagca gattgatgca gcggatgtag ttattttagc agttgatgtt
     3061 aagatttctg gtatggaacg ctttgagggt aaaaagatta tcaaggttcc aacagaagtg
     3121 gcagtcaaat ctcccaataa actgattgct aaagctgttg agattgttac gaaataactg
     3181 aaaatattta aggagaaaat atatgttgaa acacttaaac ttaaaaggtc acttattgac
     3241 agccatttcc tatatgattc caattgtttg tggtgcagga ttcttagttg ccattggttt
     3301 agcaatgggg ggtggtgttc ctgacgctct tgtagcagga aaattcacta tctgggatgc
     3361 tttagcaact atgggtggta aagcccttgg tctcttgcca gttgttattg ctacaggttt
     3421 gtcttactcg attgctggta agccagggat tgcaccaggt tttgttgttg gtctaattgc
     3481 caattctgtt ggttcagggt ttatcggtgg tatcttggga ggttatatag ctggtttctt
     3541 ggttcaagcg attattaaaa aggtcaaagt accaaactgg attaaaggtt taatgccaac
     3601 cttgattatt ccttttgtag cctctttggt aagtagtttg attatgattt atattattgg
     3661 agcgcctatc gcagccttta ccaactggtt gacgagctta ttacaaagct tgggaagtgc
     3721 ttcaaatggt ttgatggggg cagttattgg aattctcagt gctgttgact ttggtggccc
     3781 acttaataaa acagtctatg cgtttgtgtt gactttacag gctgaaggtg tgaaagaacc
     3841 attgactgct ttacaattgg tgaatactgc tacaccagtt ggatttggat tggcctattt
     3901 tatcgcgaaa ttactcaaaa aaaatatcta tactcaagag gaaatcgaaa cattgaaatc
     3961 ggctgttcct atggggattg tcaatattgt tgaaggtgta attccgattg ttatgaataa
     4021 cttggttcca ggtctcattg caacaggtat cggtggtgct gttggtggtg ctgtttcttt
     4081 gacaatgggt gctgattctg ctgtgccatt tggtggagtg cttatgttac caaccatgac
     4141 tcgtccagta gctggtattt gtgccttgtt agctaacatt gtagtcacag gacttgtcta
     4201 cgcgattttg aaaaaaccaa taaaacatgc agaaccagtt atgactgttg aagaagagat
     4261 tgatttgtca gatattgaaa ttttgtaaga gggtaacgat gtcaagaatt gaattttcac
     4321 catctttgat gaccatggat ttggacaaat tcaaagagca gattactttt ttgaatgata
     4381 aagtagcatc ttatcatatc gatattatgg atggccattt tgttcccaat attaccttgt
     4441 ctccttggtt cattcaagaa gttcaaaaaa ttagtgacac acctttatca gttcatctga
     4501 tggtcacaga cccaaccttt tgggtagatc aagttctcga tttacaatgt gagtatattt
     4561 gtattcatgc tgaagttctg aatggtcttg cttttcgttt gattgataaa attcatgatg
     4621 caggtctaaa ggctggtgtt gtccttaatc ctgaaacacc tgtttctaca atctttccct
     4681 acattgattt acttgacaaa gcaactatta tgactgtaga tccaggtttt gcaggacaac
     4741 gctttttgga gtctaccttg tataaaatcc aagaactccg tcagcttaga gttcagaatg
     4801 gttatcacta catcattgag atggatggtt cttcgagtcg taagactttc aaacaaattg
     4861 atgtggcagg accagatatt tatgttatag gtcgcagtgg attatttggt ttggatgacg
     4921 atattgccaa agcctgggat atctgttcta gagattacga agaaatgacc ggaaaaacaa
     4981 tgccaatcaa ataatggttt gagaagaaat ttattagtta ggaggaatat atgtcactac
     5041 aatcagttaa cgccattcgt tttcttggcg tagatgctat taacaaatct aattctggtc
     5101 acccgggaat tgtcatgggt gctgcgccaa tggcttatag cctatttaca aagcacctta
     5161 gaattacacc tgagcagcca aactggatta accgagatcg ctttatcttg tctgcgggtc
     5221 atggatcaat gctactgtat gctctcttgc atttaacagg gtataaggat gtatccatgg
     5281 acgagattaa aaatttccgg caatggggat ctaagacacc tggtcatcct gaagtgacgc
     5341 atacgtctgg tgtggatgcg acatctggtc cgcttggtca ggggatttct actgccgttg
     5401 gtttcgccca agcagagcgt tttttagctg ctaagtacaa caaagatggt ttccctattt
     5461 ttgaccatta tacttatgtt atcgctggag acggtgactt catggaagga gtgtctgcgg
     5521 aggcggcttc ttatgcaggt catcaagctt tagataagct tatcgtcctc tacgactcca
     5581 acgacatctg cttggatggt gagaccaaag atactttctc tgaaaatgtt cgcgtccgtt
     5641 acgatgctta tggttggcat acagttctgg tagaagatgg aacagattta gcagcaattt
     5701 ctacagcaat tgagacggcc aagttttctg gtaaaccgag tttgattgaa gtgaaaacgg
     5761 taattggtta cggctcaccc aataaaagtg gtacaaatgc tgttcatggt gcaccactag
     5821 gagcagaaga aacaggagca actcgtaagt ttttgggatg ggattacgat ccatttgaag
     5881 taccagagga agtatattct gatttcaaga caaatgtagc ggatcgtggt caggaggcat
     5941 acgatgcttg ggctagtttg gtgtctgatt acaaggttgc ttatcccgaa gttgctagtg
     6001 agattgacgc tattgtagct ggaaaatccc ctgtaaccat tactgaaaaa gacttccctg
     6061 tctatgagaa tggcttctct caagcaactc gtaattcgtc ccaagatgct attaatacag
     6121 cagcagtttt accaaccttc ttaggtggat cggcagactt agctcactct aacatgacct
     6181 acatcaaggc agatggctta caagataaat ataatccatt aaaccgcaat attcagtttg
     6241 gggtacgtga atttgccatg ggaacaatcc tcaatggaat ggctcttcat ggtggtttac
     6301 gagtttatgg cggaaccttc tttgttttct ctgactacgt caaagctgct attcggctat
     6361 cagccattca ggagttgcct gtaacttatg tctttaccca tgattcaatt gccgttggtg
     6421 aagatggtcc aactcatgaa ccagttgaac atttggcagg tttacgctca atgccaaact
     6481 tgactgttat ccgtccagcg gatgcccgtg aaactcaagc ggcttggcat catgccttga
     6541 ccagtaccac cactccaact gtcattgtct taacccgtca aaacttggta gttgaagaag
     6601 ggacagactt tggtaaggtc gctaaaggag cctacgtcgt gtatgatacc ccgggatttg
     6661 atactattat cattgctaca ggatctgagg tcaatctagc tatcaaagct gctaaggaat
     6721 tggttttaca aggtggtaaa gtacgtgtgg tatctatgcc ctcaaccgaa ctatttgatg
     6781 ctcaagatgc tacctacaag gaagacattt taccatctaa gactcgtcgt cgtgtggcca
     6841 ttgaaatggc agcgacccaa agttggtaca agtatgttgg tttggatggc gcggtcatcg
     6901 gtattgacat cttcggtgcg tctgccccag ctcagactgt gattgataat tatggattta
     6961 cggtagagaa tatcgttgct caagttaagt ccctatagaa accaattaca atgaagatac
     7021 agctgttgtc agactagcag atgtagtgat agacactaat cagatgattg gttatttaaa
     7081 aactgtaatg aaaatgtaat aatttatcta cgaaagttat agtagatagt atacacaata
     7141 gagtataccc tgaaacggtt gcgaagtacg ctaatcactt tgctactgat ctagatagtt
     7201 tctttaatca ataaacacag catccacaga ttgacttagg atattgtaag ttttttgaaa
     7261 gctagagaga aggtctctaa aattaaaaaa cgcatagtat aggatgttga aatgatgaac
     7321 tgcaccccaa aagttagaca gaaaaaaatc taacttttgg ggtgttttta ttatgaaatt
     7381 aacttatgat gataaagtta agatctatga acttagaaaa caaggatata tcttagagaa
     7441 gctttcaaat aaatttggga taaataattc taatcttagg tacatgatta aattgattga
     7501 tcgttacgga atagagttcg tcaaaaaagg gaaaaatcgt tactattctc ctgatttaaa
     7561 acaagaaatg attcataaag tctgacatga aggctggact aaagatagag tttctcttga
     7621 atacggtctc ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg
     7681 gtatactatt gttgagaaaa caaaagggag agtacctgag agcggagaat gccatcctaa
     7741 aaaagttaag agaactccga ttgaaggagg aaaaagagaa ataagaaaga cagaaattgt
     7801 tcaagaatta atgactgagt tttcgttaga tcttcttcta aaagccatta aactagctcg
     7861 ttggacctac tactatcact tgaaacagct agataaacca gataaggacc aagagcttaa
     7921 agctgaaatt caatccatct ttatcgaaca caagggaaat tatgcttatc gccgggttca
     7981 tttagaacta agaaatcgtg cttatctggt aaatcataaa agagttcaag gcttgatgaa
     8041 agtactcaat ttacaagcta aaatgcgaca gaaacgaaaa tattcttctc ataaaggaga
     8101 cgttggtaag aaagcagaga atctcattca aggccaattt gaaggctcta aaacaatgga
     8161 aaagtgctac acagatgtga cagaatttgc cattccagca agtactcaaa aactttactt
     8221 atcaccagtt ttagatggct ttaacagcga aattattgct tttaatcttt cttgttcgcc
     8281 taatttagaa taagtacaaa caatgttgga acaggcattc acagagaagc actatgagaa
     8341 tacgattctc catagtgacc aaggctggca ataccaacac gattcttatc atcggttcct
     8401 agagagtaag ggaattcaag catccatgtc acgcaagggc aacagcccag acaacggcat
     8461 gatggaatct ttctttggca ttttgaaatc ggagatgttt tatggttatg agaagaactt
     8521 tagatcttta gaaaaccttg aacaagctat tgtggactac attgattatt acaacaacaa
     8581 gagaattaag gtaaagctaa aaggacttag ccctgtgcaa tacagaacta aatccttcgg
     8641 ataaattaat tgtctaactt ttggggtgca gtacattttt ggtatatata aaatttgtag
     8701 gagctatatc tacaatttta tattcccagt ttatggatgt aacttactat attcacaatg
     8761 ttatccagtg ttttttctct aatatttaag gagtgttctg tttctcgaat aaattcttca
     8821 aagtttaacc cgtcaacttg ttcctgaaca agaaaataat catccacgat ataaaattca
     8881 tcagttaaat tagtagtata acttttatcg gctaattttt ttagcatgtg agcttcattt
     8941 tttatatcat caagagctgt ccattctcct tcagcatcat aattcacaaa aggtcttgac
     9001 tgcttgatga ttactttttg cccgtccgat tttctaattg cccgataaac atttccttta
     9061 tttgatctct taataatttt ttccattttg tatttattta ttgcagagtc cttacttgaa
     9121 acttcacatg tggtttgaaa ataaatcctt ttttcttctt ctgaaaataa atccattttc
     9181 cagctaggaa gactagggaa attttgtctc ttatcttcta catagttctt ccttttttca
     9241 tctagcaata aatagatgac ttttttattt ttttcatcat aagcttgttt ttttaaaaaa
     9301 gccccatatc tataatgaac tggagaatgc attccacatt gatagtcaga tagtattttt
     9361 ggagccttaa attctgacag tctattcgta agattacaaa tcatactctt agcttcagat
     9421 tctgacttag gatatagagt tataaatttg ttagcagtag ggctcatttc cctaggggaa
     9481 ttaatttttt ttaattcctc taaattttta acaactttaa agctacaatt atttagttgg
     9541 gatagtttat acacaatctt aaaaatattt acagcgtctt ttatttggga gcttatgtgt
     9601 attttccacc cttgtgcagg catcttatct gataaataat agtaattcca aatatcaccc
     9661 tgttggaaat ttttatcaca ttttaaacat tctttctcta ataaatccat aacaaactcc
     9721 taattattaa aataggctag agacacagca ctagcctatt ccgtaagtaa agctacttat
     9781 tttatataaa atataactag cgacctgtaa ttaaacaagt aaaagaacaa tacttaggga
     9841 gacctgtgac aatgatatct tcctctggaa gtagttctaa ttcaaacaat gtttgttcca
     9901 tgactgtatc ctcctgttat gataaattcc agaaagcata gagcaaattc gtggttcgtc
     9961 tgactaaatt tcttatctga acaccacctt caaatacaaa ttttagtcaa acttttattt
    10021 tttctaactt tctaattata ttctaccatg tgtttttata tgtcaagaat tttctgaata
    10081 aactgtttat tttttatttt ttgacatttt tgcaaattgt ttaaaatctt tcaattggat
    10141 tcacttatta ctttaggaca ttgaaacttc tttcgccaga ggtgcttata ttactgggaa
    10201 gttgaagatt gaaatgtggt ataataagag gtaatagagt tttggaaagt gagagaagat
    10261 gatttcaaag agattagaat tggtagcttc ctttgtgtca cagggggcta ttttactaga
    10321 tgtgggaagt gaccatgctt atctgcctat cgagttggtt gagagaggcc aaatcaaaag
    10381 cgctattgca ggtgaggtgg tggaaggtcc ctatcagtct gcggttaaaa atgttgaggc
    10441 tcacggccta aaggagaaaa tccaagtccg tttagccaat ggcttggcag cttttgaaga
    10501 gactgaccaa gtgtctgtca ttaccattgc tggcatgggt ggtcgtttga ttgctaggat
    10561 tttagaagaa ggtttgggga agttagctaa tgtagagcgt ttgatcctcc agcccaataa
    10621 tcgtgaagac gacttgcgta tctggctaca ggatcatgga ttccagattg tagcagaaag
    10681 catcttagaa gaagctggaa agttttatga gattttggtg gtggaagcag gacaaatgaa
    10741 gctatcagcc agtgatgttc gctttggtcc cttcttgtcc aaagaagtca gtccagtatt
    10801 tgtccaaaaa tggcaaaaag aagctgagaa gctagagttc gccctcggac aaatcccaga
    10861 aaaaaatctg gaagaacgtc aagttctagt agataagatt caagctatca aggaggtgct
    10921 ccatgttagc aagtgaagtg atccaagctt atgaagcctt ttgccctcag gaattttcta
    10981 tggagggaga cagccgtggt ctgcaaattg gcactttaga caagggtatc caaagggtca
    11041 tggttgccct cgatattcgt gaagagacag tggcagaggc cattgaaaag ggtgtggact
    11101 tgattatcgt caagcacgcg cctatctttc gtcctatcaa ggacttgctt gccagccgtc
    11161 cgcaaaatca gatttacatc gacctgatta agcatgatat cgcagtttat gtcagccata
    11221 ccaatattga tatcgttgaa aatgggctca atgactggtt ttgtcagatg ctaggaatcg
    11281 aggagacgac ttatctgcag gaaacaggtc cagaacgtgg aattggacgt attgggaata
    11341 ttcagcctca gacattttgg gaattggccc aacaggtcaa gcaagtcttt gacctagata
    11401 gccttcgaat ggtgcattat caagaggatg atttgcagaa gcctatttca agagtagcaa
    11461 tttgtggtgg aagcgggcag tctttctata aggatgcttt ggcaaagggg gcagatgtct
    11521 atatcactgg tgatatctac taccatactg ctcaggatat gctatctgat gggttgcttg
    11581 cattggaccc aggtcactat atcgaagtga tttttgtaga aaaaatcgca gcacttctta
    11641 gtcaatggaa ggaagataag gggtggtcta ttgatatctt gcctagtcag gcatcaacca
    11701 atcctttcca tcatatctag ttagaaagtg aagacaatga aaaaagttgc cattattgga
    11761 gcaggaattg tgggagcaac agctgcctac tacctctcga gagaaagtga cctagaggtg
    11821 accgtttttg accatgagca aggtcaagcc accaaggccg cagcaggaat tatcagtcct
    11881 tggttttcca aacgccgtaa taaagcctgg tacaagatgg cgcgcttggg ggctgatttt
    11941 tatgtggatt tattagctga tttagagaaa tcaggacaag aaatcgactt ttaccagcgt
    12001 tcgggagtct ttctcttgaa aaaggatgaa tccaatttgg aagaacttta tcaactggcc
    12061 ctccagcgca gagaagaatc tcccttgata gggcaattag ccattctgaa ccaagcctca
    12121 gctaatgaat tattccctgg tttgcaggga tttgaccgcc tgctctatgc ttctggtgga
    12181 gcgagagtag atggccaact tttagtgact cgtttgctgg aagtcagtca tgtcaagctg
    12241 gtcaaagaaa aagtgactct gacaccgtta gcatcaggct accagattgg tgaagaggag
    12301 tttgagcagg ttattttggc gacgggagct tggttggggg acatgttaga gcctttaggt
    12361 tatgaagtgg atgtccgtcc tcaaaaagga caactacgag attatcagct tgcccaagac
    12421 atggaagatt accctgttgt catgccagaa ggggagtggg atttgattcc ctttgcaggt
    12481 gggaaattat ccttaggcgc tacccacgaa aatgacatgg gatttgattt gacggtagat
    12541 gaaaccttgc tccaacaaat ggaggaggcc accttgactc actatctgat tttggctgaa
    12601 gctacttcaa aatctgagcg tgttggaatc cgtgcctaca ccagtgattt ctctcctttc
    12661 tttgggcagg tgcctgactt aactggtgtc tatgcagcca gtggactagg ttcatcaggc
    12721 ctcacaactg gtcctatcat tggttaccat ctagcccaac tgatccaaga caaggagttg
    12781 accttggacc ctctaaatta cccaattgaa aactatgtca aacgagtaaa aagcgaataa
    12841 tactcaataa aaatcaaaga acaaactagg aaactagccg cgggttactc aaagcactgc
    12901 tttgaggttg tggatagaac tgacgaagtc agctcaaaac actgttttga ggttgtggat
    12961 agaactgacg aagtcagtaa ccatacccat ggcaaggcga cgttgacgta gtttgaattg
    13021 gattttcgaa gagtataagc attttactga aattttatca ggtagtttag gatggcaaat
    13081 gacattccct gtcaaaaatg gtaaaataag aaaaaataat ctgagaatcg aggaaaaaag
    13141 atgcaagaaa agattttggt aactggtggt gccggtttta ttggaaccca cactgttatt
    13201 gaattgatcc aagcaggtca tcaagttgtt gtggtggata atcttgtcaa tagcaatcgt
    13261 aagagtttag aagttgttga aggaatcaca ggagttgaga ttcctttcta tgaggcagat
    13321 attcgtgaca cagataccct cagagatatt ttcaagcagg aagaaccgac tggtgtcatt
    13381 cactttgctg gtttgaaggc tgttggcgaa tcaacacgta tccctcttgc ctactatgac
    13441 aacaatatcg ctggaactgt cagccttttg aaagccatgg aagaaaacaa ctgtaaaaac
    13501 atcatcttca gttcttctgc gacagtttac ggggatccgc acacagtgcc catcttggaa
    13561 gatttcccac tttcagtgac caacccatac ggtcgtacta agctcatgct agaggaaatt
    13621 ttgactgata tttacaaagc agactcagaa tggaatgttg tcttgcttcg ttactttaac
    13681 ccaatcggag cccatgagag tggtgatttg ggagaaaatc caaacggtat tccaaacaat
    13741 ctcttgccat atgtgactca agtagccgtt ggaaaattag agcaagtgca agtgtttgga
    13801 gacgattacg atacggaaga tggaacaggt gttcgtgact atatccacgt tgtcgatttg
    13861 gctaagggtc acgttgcagc tttgaaaaaa atccaaaaag gttcaggact aaacgtttat
    13921 aaccttggaa ctggtaaagg ttactcagtt cttgaaatta tccaaaacat ggaaaaagcg
    13981 gtgggccgtc ctattcctta ccgcatcgta gaacgtcgcc caggtgatat cgctgcctgc
    14041 tactcagacc cggcaaaagc taaagcagaa ctcggttggg aagcagaact cgacatcacc
    14101 caaatgtgtg aagacgcatg gcgttggcaa agcaagcatc caaatggatt tgaagactaa
    14161 gatgatgatt tcaatcatcg tcccttgttt aaacgaagag gaagtacttc ctctttttta
    14221 tcaggctctg gaagctttac ttccagattt ggaaacagaa atcgagtatg tctttgtcga
    14281 tgatggatca agtgatggga ccttggaact cttaaaggcc tatcgggagc aaaatccggc
    14341 agtccattat atttctttct ctcgaaattt tggcaaagaa gcagcccttt atgcaggctt
    14401 gcaatatgcg acaggagatt tggtggtggt gatggatgca gacctccaag atcctcctag
    14461 tatgttgttt gagatgaaaa atgtattaga caaaaatgta gacttggact gcgttgggac
    14521 acggagaact agtcgggagg gagaaccctt ctttcgcagt ttctgtgctg ttctctttta
    14581 tcgcctcatg caaaaaatca gcccagtagc tctgccgtcg ggtgtccgtg attttcgtat
    14641 gatgagaagg tctgtagtcg atgccatttt aagcttgacc gagtccaatc gtttttcaaa
    14701 ggggctcttc gcctgggtgg gtttcaaaac ctactatttg gactatccaa atgtcgaaag
    14761 gcaggctggc aagacaagtt ggagttttag gcaactcttt ttctactcca ttgaagggat
    14821 tattaatttt tcagattttc ccttgattat agcctttgta gctggtctcc tatcttgttt
    14881 tctttctctg ctgatgacct tttttgttgt ggttcggacc ctcattttgg gcaatccgac
    14941 atctggttgg acctctctga tggctgttat tctctttctt ggaggcattc aactcttgac
    15001 cattgggatt ctcggcaagt atatcagtaa gatttattta gagactaaaa aaagaccact
    15061 ttatcttatc aaagaaaaaa gtgaccttcc tgattttaca gaaaaaaata aagtgaaaag
    15121 actataattt tacatggaaa tgtgctaaac tagagggagt ggattatcgc cattgattta
    15181 ggcttcctgg tttccaatca gggcgcgagt tgggcaattt ttaacagcct ctaagatatc
    15241 ctgactagga gaaatttctt tttccagttg gtcagggtca tcgtaaaaac gcacgattcc
    15301 attatcgtgg taatcaaata aatcagaata agtttggcaa agcccacagg cgatacatcg
    15361 ttcaggtata agtgtgattt tcatgttcat atttatattg taataagaaa agtaaaaaaa
    15421 aacaaggagt aaggtatggc aaaagaaccg tggcaagaag atatctatga tcaagaagaa
    15481 tcaagagcag agcgtcggca tcgaaaccac ggaggggctg ataggatggc taatcgtatt
    15541 ttgacgatcc tagctagtat tttctttgta attgtggtgg tgatggtcat cgttctcatc
    15601 tatctatcat cgggggggag taatcgcaca gcagccttaa aaggctttca tgattctgat
    15661 gccagtgtag tacaaatctc atcttcaagt agttctcagc ctgagcagag ttcagagcca
    15721 gaatctactt ctagtagttc agaagaagct gctaatcctg aaggaacgat taaagttctc
    15781 gcaggagaag gggaagcagc tattgccgct cgtgcaggaa tctccattgc tcagttagag
    15841 gccttgaatc ctgggcacat ggctacagga tcttggtttg ctaatccagg tgatgttata
    15901 aaaataaaat aggagccgat atgaaaacaa ttcaaattgc tattgatggt cctgcttcca
    15961 gcggtaagag tacggtcgca aagattattg ctaaggattt tggattcacc taccttgata
    16021 caggagctat gtatcgtgca gcgacctata tggctcttaa gaaccaatta ggagttgaag
    16081 aagtcgaagc ccttctagcc ttgttggacc agcatccaat cagctttgga cgttcagaaa
    16141 ctggagacca gcttgttttt gtaggagatg tggatattac ccatcctatc cgtgaaaatg
    16201 aagtgaccaa tcatgtttct gctattgcag caattcctca agtgcgtgag aaactggttt
    16261 ctctccaaca agaaattgcc cagcaaggcg ggattgtcat ggatggtcgc gatattggaa
    16321 ctgttgtatt gccacaagca gaattgaaaa ttttcctagt agcttctgtt gatgagagag
    16381 cagagcgtcg ttacaaggaa aatattgcca agggaattga aacagacctt gaaaccctaa
    16441 aaaaggaaat tgctgcgcgt gactacaagg atagtcatcg tgagacttct cctctcaaac
    16501 aagcagagga tgctgtctac cttgatacaa ctggtttgaa cattcaagaa gtagttgaaa
    16561 aaatcaaagc agaagctgaa aaaagaatgt aaatgtagaa aagccgatag attgatatcg
    16621 gtttttttct aaatttgtca aaatgctgat tttagggtat aatagttgct gttcgagaaa
    16681 ttttgatttt caaagaatag tgaatgatga taaggagaaa ccatgaacaa tttaccaaat
    16741 tgtccaaaat gtaactcaga gtatgtctac gaagacggtg ccctactggt ttgcccagag
    16801 tgtgctcatg agtggaatcc tgctgaagtt gcagaagtag aagaaggtct tgtcgctatc
    16861 gatgccaacg gaaataaatt ggctgatggt gatacagtaa ctctcatcaa ggacttgaaa
    16921 gtaaaaggtg cgccaaaaga tttgaaacaa gggacgcgcg tgaaaaatat ccgcatcgta
    16981 gaaggcgacc acaatatcga ctgtaagatt gatggttttg gtgccatgaa acttaaatca
    17041 gagtttgtga ggaagattta agcatgtcaa aacactataa acttgtattt tatagccgta
    17101 tcttcttgtt tctagcggct tttacgggag tttatcttga aattactaag catggtggtt
    17161 ttgggatgct tctctattac acggttctgt ccaacctctt ggtaactatt tttacccttt
    17221 atcttctaaa ggttatgagc cgtgtaggtg aaaattggca aaggccaagt ctcttgcgct
    17281 taaaaggtgg ggtcaccatg agtatcatga ttacctgtgt gatttaccat ttcctcttgg
    17341 cgcccattgc gactaatttc tataccctag aaaatttcct ttgccactat atcgttccca
    17401 tctggttttt agcggatacc ctcttttttg acaaacaggg tcaatacaag atttgggatc
    17461 cagcagtgtg gaccatttta ccctttctgt atatgatgtt tgctcttttt aatggcttgg
    17521 ttctaaaact caatattcca aatgccaagg ataatccttt cccttacttc tttttgaatg
    17581 tgaacaaggg ttggaatgtt gtgtttaagt ggtgtctgat tatctttgtt gcctatatgg
    17641 tagcaggatt tattttctac tttatcaagc aaatcaagag aaagtcatcc taatactctt
    17701 cgaaaatctc ttcaaaccac gtcagtttcc atctgcaacc tcaaaacact gttttgaaca
    17761 acctgcggct agcttcctag tttgctcttt gattttcatt gagtataaga tacaagggat
    17821 tcattttcga atttaagaag gagtctacaa accaatgagg cttcttcttt tcttgcttat
    17881 ttgataacta tcaaaaaagt agaagattaa gagaaatata gaaagagatt tcatgaaaca
    17941 atttttagaa cgggccagca ttttggctct ctccctcgtt ttgattacct ccttttccat
    18001 ttcgagtgcc ctaccagcca tgtttgacta ttatcagggt tattctaagg aacaaattga
    18061 gctcttggtg agcttgcctt cctttggaat catgatgatg ttactgctaa atggtttctt
    18121 agaaaaaata tttcctgagc gcttacagat tagtttgggc ttgctgattt tatcattgag
    18181 cggtacagct cccttctggt accaagccta tccctttgtc tttggaacac ggcttctctt
    18241 tggtttgggt cttgggatga tcaatgccaa ggccatttct attatcagtg aacgctacca
    18301 aggaaaaagg cgaattcaga tgttagggct acgcgcttct gcagaggtcg ttggagcttc
    18361 tctcattacc ttggccgtcg gtcagttgtt ggcctttggt tggacagcta tctttctagc
    18421 ctatagtgct ggatttttgg tgctgcccct ttatctgctc tttgtccctt atggaaaatc
    18481 aaagaaagaa gtcaagaaaa gagcgaagga agcaagtcgt ttaactcgag aaatgaaagg
    18541 cttgattttt accttagcta tcgaagcggc agttgtagtt tgtaccaata cagctattac
    18601 catccgtatt ccaagtttga tggtggaaag aggattgggg gatgcccagt tatctagttt
    18661 tgttcttagt atcatgcagt tgatcgggat tgtggctggg gtgagttttt ctttcttgat
    18721 ttctatcttt aaagagaaac tgctcctctg gtctggtatt acctttggct tggggcaaat
    18781 cgtgattgcc ttgtcttcat ccttgtgggt ggtagtagca ggaagtgttc tggctggatt
    18841 tgcctatagt gtagtcttga cgacggtctt tcaacttgtc tctgaacgaa ttccagctaa
    18901 actcctcaat caagcaactt catttgctgt attaggctgt agtttcggag cctttacgac
    18961 cccattcgtt ctaggtgcaa ttggcttact aactcacaat gggatgttgg tctttagtat
    19021 cttaggaggt tggttgattg taatctctat ctttgtcatg tacctacttc agaagagagc
    19081 ttaggattga ttcctaagtt tttcttttga tactcaatga aaatcaaaga gcaaactata
    19141 gttgattgag tttggaatag tacgctgtag ctgctaaaat atttctagaa attaatttga
    19201 ctttcctaat agagttgttc atatcttatt tcaatctact atagaaagct agccgcaggt
    19261 tgctcaaaat agtgtgttga ggttgcagat ggaaactgac gtggtttgaa gagattttcg
    19321 aagagtatga gatttttata cccatacaat atttgtttta ggtccttgtt tacagactaa
    19381 tttcttgata aaatagaggc tatgacgaga tataaagcaa ctatttccta tgatggttat
    19441 gcctttgctg gctttcagcg ccagcctcat gcgcgtagcg ttcaggaaga aattgaaaaa
    19501 accttgacca gattaaataa agggcaagcc attactgttc acggtgctgg taggacagat
    19561 agtggggttc atgccctggg acaggtgatt cattttgacc tgccttatca gatggatgag
    19621 gagaaactcc gttttgcctt ggatacccag tctcctgaag atattgatgt gatttcgatt
    19681 gagcttgtgg cagatgattt tcattgccgt tatgccaagc atagcaagac ctatgagttt
    19741 actgtggata gaggacgtcc caaaaatccg atgcgccgtc actatgccac ccactttccc
    19801 tacccactcg atgtggaacg aatgcagatt gcaatcaaaa agctagaggg aacccatgat
    19861 tttaccggtt ttacagcctc tgggactagt gtagaggata aggttcgcac catcacagaa
    19921 gctagtttaa tagtcgatga gacaggacaa tttttgacct ttaccttttc aggaaatggt
    19981 ttcttgtata aacagattcg caatatggtg gggactcttc tcaaaatcgg taacaaccgc
    20041 atgccagtag agcagattga tctcatcttg gagaagaagg acaggcaact tgcaggtccc
    20101 actgcagcac caaatggttt gtatttaaag gagattcgtt atgaagaata atcgtatttt
    20161 agcactttct ggaaatgata tttttagtgg tggtggactg tcagctgatt tggctaccta
    20221 taccttgaac ggcttgcatg ggtttgtagc agtgacttgt ttgacagcct tgacagaaaa
    20281 aggatttgaa gtctttccaa ctgatgatac catttttcaa catgaattag atagcttgcg
    20341 tgatgtggaa tttgggggaa ttaagattgg tcttctccct actgtcagcg tggctgagaa
    20401 ggccttggac tttatcaaac aacgcccagg agtacctgtg gtgttggatc ctgtcttggt
    20461 ctgcaaggaa acgcatgatg tagctgtcag tgagctctgc caagagttga ttcgcttttt
    20521 cccttatgtc agtgtgatta cgcctaatct cccagaagca gaattattat ccggtcagga
    20581 aattaaaacc ttggaagaca tgaaaactgc agcgcagaaa ttgcatgatt taggagcgcc
    20641 agcagtcatt atcaagggag gcaatcgtct tagtcaggac aaggctgtgg atgtctttta
    20701 tgatggacag acctttacta tcctagaaaa tccagttatc caaggccaaa atgctggtgc
    20761 aggttgtacc tttgcctcta gcattgccag tcacctggtt aaaggtgata aacttttgcc
    20821 agcagtagaa agctctaagg ctttcgttta tcgtgctatt gcacaagcag atcagtatgg
    20881 agtaagacaa tatgaagcaa acaaaaacaa ctaaaatcgc ccttgtatcc ctattaaccg
    20941 ccctttctgt ggttctaggt tatttcttaa aaatcccaac acctacagga attctaactc
    21001 ttttagatgc tggtgtcttc tttgcggcct tttactttgg tagtcgtgaa ggagcggtag
    21061 tcggaggact agcaagtttc ttgattgacc tcttatcagg ctaccctcag tggatgttct
    21121 ttagcttggt caaccatggc ttgcagggat ttttcgcagg atttaaagga aaaagtcagt
    21181 ggttaggcct tattttagca actattgcca tggtaggagg ctacgccttg ggttctgctt
    21241 tgatgaatgg ctgggcagca gccctcccag aaattctacc gaattttatg caaaatatgg
    21301 tagggatgat tgtaggattt attcttagtc aaagtatcaa gaagattaag taatactctt
    21361 cgaaaatctc ttcaaaccac gtcagcttcc atctgcaacc tcaaaacact gttttgagca
    21421 gcctgaggct agctttctag tttgcacttt gattttcatt gagtataaag aggttgagta
    21481 aaaagtattt caaataagaa aaacgcatag tatcaggtgt tgaatgaact gc
//
LOCUS       DBDESG010000040        21513 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.040, whole genome shotgun sequence.
ACCESSION   DBDESG010000040 DBDESG010000000
VERSION     DBDESG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.040"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..372
                     /locus_tag="ACKB38_001416"
     CDS             <1..372
                     /locus_tag="ACKB38_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGL7980173.1"
                     /translation="FCLVLVSIDYINCLSETTDKNWHKSDRIFVTNTGKTVHSSILSK
                     SLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHPDSEVTTSIYTH
                     VTKNMKDEAINVLDKVMKKIF"
     gene            complement(692..868)
                     /locus_tag="ACKB38_001417"
     CDS             complement(692..868)
                     /locus_tag="ACKB38_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980174.1"
                     /translation="MDFLNHSFDTKKVINTKINAVNSKNNVGKNFIDVYREMKEVPNN
                     KIHQSKVNVALIKK"
     gene            complement(871..2061)
                     /locus_tag="ACKB38_001418"
     CDS             complement(871..2061)
                     /locus_tag="ACKB38_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980175.1"
                     /translation="MTAEIGILNKNGVVLASDSAVTLSDGKNSKVFNSARKLFTLSKE
                     HSVGIMIYGNASFMEIPWEVILNEFKQAIGTDLLDNTAQYVEKLIEFLISFKHLQVED
                     LLRNYIVRSTRSILDSIAYEAQETADLRVSNGETITLDDFNKILLNAITNFSLEISKV
                     EAESNFEFFEAELELIKSIVDDVFKSFPHTDDEVEVIAKSLYKAIFIGYDSTNITGLV
                     IAGYGTDEIFPSIRQIELYGIFSKRLIWKVINESVINHHKTCHIIPFAQSEMVETIMN
                     GIDPNLNVYIAEQVSSVMEKNGLGDEIENIFENISSIQQKYYINPIIDLIGMQPLNEM
                     ASTAKTFIELTSFKRKIVNTLETVGGPVDVLAISKGEGPIWIDRKYYFDIDKNLDYRM
                     RKES"
     gene            complement(2079..2372)
                     /locus_tag="ACKB38_001419"
     CDS             complement(2079..2372)
                     /locus_tag="ACKB38_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980176.1"
                     /translation="MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKG
                     ISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEHEQV"
     gene            complement(2362..2727)
                     /locus_tag="ACKB38_001420"
     CDS             complement(2362..2727)
                     /locus_tag="ACKB38_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGL7980177.1"
                     /translation="MHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIEL
                     LSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWMDGSFVLLHHFMKRTQKTPKREI
                     EQAKRELADLKERGLDNEK"
     gene            complement(2924..3334)
                     /locus_tag="ACKB38_001421"
     CDS             complement(2924..3334)
                     /locus_tag="ACKB38_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980178.1"
                     /translation="MTKKKIERISVIHREKILWLKWYFMRDKEQPKYSVLERKMFDAA
                     KNQDMLAYQKYATIKQITDIRVQTSEADILEAVKEVYVYNHMNVIGACQRILFISQSP
                     AYDKLNKWFNIYSDLYFSVVPLPKMGVYHEMVGI"
     gene            complement(3352..3663)
                     /locus_tag="ACKB38_001422"
     CDS             complement(3352..3663)
                     /locus_tag="ACKB38_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980179.1"
                     /translation="MPEFIIVEGNNDLGEFFQIDGELFSDNELLENLKKWREWEVPVI
                     IDDWCNRILNEDETEILYFPTHEDKMNYIRVEKDLEPLYHTSNKIYATISKSEWLELL
                     N"
     gene            complement(3671..4099)
                     /locus_tag="ACKB38_001423"
     CDS             complement(3671..4099)
                     /locus_tag="ACKB38_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGL7980180.1"
                     /translation="MAGNENDNLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRIKQDEMMDKTTQMLYLASSNAVSVLNDIMMDSKVNPFIRTQAAKAILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGNQRVTRTQGVIK"
     gene            complement(4146..4796)
                     /locus_tag="ACKB38_001424"
     CDS             complement(4146..4796)
                     /locus_tag="ACKB38_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980181.1"
                     /translation="MTENKDDKLLKMMEKGFVLYSKNGIIKYIEIPEHGSIKLKAQDG
                     QLVYKEVIIGKVYAEQELLKYKKEGENYSQNTAQLLYQQLEKEKNAELANHESKEEPI
                     TADDAAELSLLSSIKLTVAEMREYLEKYKNKPLALRKLEDIMDNDTTLAYIEIDMEQF
                     SQQQRLEKLVHFLDRKIDYFHGGLLINGDKIDLVQHEMIVEGNSEAMDAELQNYLA"
     gene            complement(5045..5929)
                     /locus_tag="ACKB38_001425"
     CDS             complement(5045..5929)
                     /locus_tag="ACKB38_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGL7980182.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EAIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYMQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFGIKYKRYSLNQLKSNM"
     gene            complement(5941..6387)
                     /locus_tag="ACKB38_001426"
     CDS             complement(5941..6387)
                     /locus_tag="ACKB38_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGL7980183.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYIKVILN
                     RWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(6384..6836)
                     /locus_tag="ACKB38_001427"
     CDS             complement(6384..6836)
                     /locus_tag="ACKB38_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980184.1"
                     /translation="MTETTYENLTRRIDRISAEMREISEENGLRILSLLESQTKSIKD
                     ELSRLLWIELPELNESHKIEAVSKSTTGMFFAPGIFEMDAMRKAFFKRQAKHFFENEA
                     EQQAYIEHAEKEYLEATVTLKDILFNSKNGTQKANKSCLIEKFEEAMQ"
     gene            complement(6871..7488)
                     /locus_tag="ACKB38_001428"
     CDS             complement(6871..7488)
                     /locus_tag="ACKB38_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGL7980185.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAEVQHHTITRLIRENKADFEEL
                     GILGFKIHKLDTRGQPKKSYILNEQQATFLITYLKNTETVRQFKLNLVKAFFEMREEL
                     SEIRLQRALEKPKRKTLHDSIETWPNAPKHAHSTMNNLLLKAVTDMNAKQLREERGGY
                     NGIDSLTSEELEQYQAFEDMAIAMIELKMSYQEIKTMMFRSKKIS"
     gene            complement(7693..7845)
                     /locus_tag="ACKB38_001429"
     CDS             complement(7693..7845)
                     /locus_tag="ACKB38_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980186.1"
                     /translation="MKIVTFKPTKQIDDGFYLPGIDILFVSDKADAKDKEDVILFLSR
                     NGLNKS"
     gene            complement(7856..8122)
                     /locus_tag="ACKB38_001430"
     CDS             complement(7856..8122)
                     /locus_tag="ACKB38_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980187.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLGLKRYQTPMDGAKKVFYRPSDVYLFLAIK"
     gene            complement(8222..8575)
                     /locus_tag="ACKB38_001431"
     CDS             complement(8222..8575)
                     /locus_tag="ACKB38_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980188.1"
                     /translation="MSKENKMRGYRNMLGLTQEKLGKKLGISKQSYYNKESGKTQFSD
                     KEKLKIKNLLIPLFPDITIEDIFFKQKYAKVKSAKNGIKAKIKAVYRRPNQRTRLRKN
                     NKTQAKARRVVLLGI"
     gene            8695..9543
                     /locus_tag="ACKB38_001432"
     CDS             8695..9543
                     /locus_tag="ACKB38_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980189.1"
                     /translation="MKINNKLVGERIQNIRLSHGDSMEKFGEKFNTSKGTVNNWEKGR
                     NLPNKENLLKIASIGKMSVEELLYGDYNTYLHLKIMDLAPECIKNYDEYNSLHDDITN
                     KALQIAQNTISKIDYQISDETIKKFIDLAIEQSRDLQGNLLKNNLSNFDYSLFGGTRN
                     REYFYFGYRLTYKEKYLYTALLAILNESTKTIYIDDINTARFFNFFGNDIPNSSLKKI
                     DYIAPSEIVSFSTYVRQRSKVIPKILEHILKSSFLLENIDVSGYTVNILEDQLTKHRT
                     IKISKN"
     gene            9618..10781
                     /locus_tag="ACKB38_001433"
     CDS             9618..10781
                     /locus_tag="ACKB38_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGL7980190.1"
                     /translation="MNIKEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMEGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDALDQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAINNL"
     gene            complement(10935..12239)
                     /gene="eno"
                     /locus_tag="ACKB38_001434"
     CDS             complement(10935..12239)
                     /gene="eno"
                     /locus_tag="ACKB38_001434"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGL7980191.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            12403..12849
                     /locus_tag="ACKB38_001435"
     CDS             12403..12849
                     /locus_tag="ACKB38_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGL7980192.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(12846..13961)
                     /locus_tag="ACKB38_001436"
     CDS             complement(12846..13961)
                     /locus_tag="ACKB38_001436"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGL7980193.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASFYLERTAANIGHLLNMCKI"
     gene            complement(13970..14203)
                     /locus_tag="ACKB38_001437"
     CDS             complement(13970..14203)
                     /locus_tag="ACKB38_001437"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGL7980194.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAKLG
                     IAFCAKEVLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(14244..14615)
                     /locus_tag="ACKB38_001438"
     CDS             complement(14244..14615)
                     /locus_tag="ACKB38_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HGL7980195.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(14674..16107)
                     /gene="glgA"
                     /locus_tag="ACKB38_001439"
     CDS             complement(14674..16107)
                     /gene="glgA"
                     /locus_tag="ACKB38_001439"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGL7980196.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(16104..17243)
                     /gene="glgD"
                     /locus_tag="ACKB38_001440"
     CDS             complement(16104..17243)
                     /gene="glgD"
                     /locus_tag="ACKB38_001440"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGL7980197.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(17233..18375)
                     /locus_tag="ACKB38_001441"
     CDS             complement(17233..18375)
                     /locus_tag="ACKB38_001441"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGL7980198.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(18365..20293)
                     /gene="glgB"
                     /locus_tag="ACKB38_001442"
     CDS             complement(18365..20293)
                     /gene="glgB"
                     /locus_tag="ACKB38_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGL7980199.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSAIKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            complement(20776..>21513)
                     /locus_tag="ACKB38_001443"
     CDS             complement(20776..>21513)
                     /locus_tag="ACKB38_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGL7980200.1"
                     /translation="STPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVA
                     GAFSYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASAD
                     FIWGLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVAS
                     VEEAIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVK
                     GSGAGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT         6485 a         4478 c         3456 g         7094 t
ORIGIN      
        1 ttttgtcttg ttcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag
       61 aattggcaca aaagcgaccg tatttttgtt accaatacag gaaaaacagt tcatagttct
      121 atcttgagca agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat
      181 ctgtcccctc acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct
      241 ttaaaaacaa tcacggacag ggttggtcat cccgactctg aagtcactac ttccatctac
      301 acccacgtca caaagaacat gaaagatgaa gcaatcaatg tactggataa agttatgaaa
      361 aagatttttt aaaaagtttt gtcccttttt tgccctctaa atacaaaaat agcccttcgg
      421 ataaaatccg aggggctaga aacgttgtta aatcaacggc cgaacttttg aatttcatgg
      481 ttcgggataa aatagttcac tgaactattt tattttttaa ggttatcata atatcaaata
      541 gttcaattaa atacgctaaa ttactaatat actttttacc tttttcattc taaaatgtaa
      601 agtacaaaca attacaatat actagagggg gagtaaaaaa ggtattaaat cgatgagttc
      661 agcaggcaag aaaatagcac ctttacgggt gctatttttt aattaacgcc acgttaactt
      721 ttgattgatg aattttattg tttggcactt ctttcatttc acggtaaaca tcgatgaaat
      781 tctttccaac attatttttg gagttaactg catttatttt tgtattaata acttttttag
      841 tatcgaaaga atggtttaag aaatccataa ctaactctcc tttctcatcc tgtaatcaag
      901 atttttatca atgtcaaaat agtattttct atcaatccaa attggtcctt ctcctttaga
      961 aatagcaagt acatctaccg gacctcctac tgtttcaaga gtgttgacaa tttttctctt
     1021 aaatgaagtt aattcaataa atgttttagc tgtactcgcc atttcattaa gtggttgcat
     1081 tccaataagg tctattatag gatttatata atatttttgc tgtatagatg atatattttc
     1141 aaatatattc tcaatttcat cacccaatcc atttttctcc ataactgatg atacttgctc
     1201 tgcgatatat acatttaagt taggatctat accattcata atcgtctcaa ccatctctga
     1261 ctgtgcaaaa gggattatat gacaagtttt atgatgattt atcacacttt cattaataac
     1321 tttccaaatt aatcgtttag aaaaaattcc atataattca atttgtctta tagatggaaa
     1381 tatctcgtct gtaccataac ctgctataac taatccagtt atgtttgttg agtcatatcc
     1441 aatgaaaatc gctttatata aagatttagc aataacttca acctcatcat cagtatgagg
     1501 aaaggattta aaaacatcgt ctacaatgct ttttattaac tctaactcag cttcaaaaaa
     1561 ttcaaaatta ctttcagctt ctacttttga aatttctaaa ctaaaattag ttatagcatt
     1621 taataaaatt ttattaaaat catctagagt gatggtttca ccattagaaa ctcttaaatc
     1681 agctgtttct tgcgcttcat aggcaatgct gtccaaaata cttcttgtac ttctgacaat
     1741 ataatttctt aataaatcct caacttgtag atgtttaaag gaaattaaaa attctattag
     1801 cttttcaacg tattgggcag tattatctaa taaatctgtg ccaatagcct gcttaaactc
     1861 atttaaaatt acctcccacg gaatttccat aaacgaagcg ttcccatata tcatgatccc
     1921 cacggaatgt tcttttgata aagtgaataa ttttcgggcg ctattaaaaa cttttgaatt
     1981 tttcccgtct gataaggtta cagcgctatc agaagccaat acaacaccat ttttatttaa
     2041 tattccaatt tctgctgtca aaatatcacc taaactttct aaacctgctc atgctctaat
     2101 ggtacaacag ctaaggtctt accaagactt gccaacactt ttaatactgt atcaagttgt
     2161 gggcttgtct ttcctgtttc cattctagcg ataactggct gactaacacc gctcatctcc
     2221 tctagtttct tctgactaat acccttttca tttctagcct cgataagctc actcatgata
     2281 gccacgcgca tatcactttc caaaatttcc tctttgctga ataattcagc tcttacatct
     2341 ttccagttac taccaatagc attatttttc attgtctaaa cctctttctt ttaaatctgc
     2401 aagttcacgt ttagcttgct caatctctct tttgggtgtt ttctgtgtcc ttttcataaa
     2461 atgatgcagt aaaacaaaac taccatccat ccaagcaaca aataaaattc tatctctaag
     2521 tggtctcagc tcccaaattt cagcatctaa atgcttaata tatggttcgc ctgcgcgtgt
     2581 tccatgttgg cttaacaact caatataatc attaatttta ttaagcttaa ttctgctatc
     2641 tttccctttt ttactggtaa gctctcgcat ataatcaaaa acaggctcat tgccgttttt
     2701 atccttgtaa aaatagatat tatgcactat taacacctct tcctaataac aattataacc
     2761 taaaagttat tgtttgtaaa tacttttaag ttattaaaat aaaaagcacc tagtttccta
     2821 gatgctagca caatgacacg gattcgcacc gtggctacct ctatcaaggt gtactccttc
     2881 tatactatcc cttgtgcttt agaatattat accacacaat caactagata cctaccatct
     2941 catgatatac ccccattttg ggcaagggta caacgctaaa atacaaatca gaatagatat
     3001 taaaccactt atttaactta tcataagctg gtgattgact gataaataat atccgctgac
     3061 aagctccgat aacattcatg tgattgtaca cataaacctc ttttacagcc tctaaaatgt
     3121 cagcctcact tgtttgtacc ctaatatctg ttatctgctt gatagttgcg tatttttgat
     3181 aagctagcat atcttgattt ttagcagcat caaacatttt acgctcaagg acactatact
     3241 taggttgttc tttatctcgc atgaaatacc acttgagcca taaaatcttt tctcggtgta
     3301 ttacagaaat acgctcaatt ttcttctttg tcattgctac ctcctaaatc atcaatttaa
     3361 caattctaac cactcacttt tagaaatagt tgcatagatc ttgttcgatg tatgatacaa
     3421 aggttctaaa tctttttcca ccctaatata gttcatctta tcctcatgag taggaaagta
     3481 tagtatttcc gtttcatcct cgtttaggat acgattgcac caatcatcaa taataactgg
     3541 cacttcccac tcacgccatt ttttaaggtt ttctaaaagt tcattatcac taaatagctc
     3601 gccatctatt tggaaaaatt cccctaagtc attgtttcct tcaacaataa taaactctgg
     3661 catatttcta ttacttaata actccttgag ttcttgtaac tctttgattt cctttagata
     3721 cttcctcaat ttccaacctc aattcttcaa tctgccttac tactccaaaa atttcatggg
     3781 tcttataaga ttgttcaagt atagcctttg ctgcttgagt tcttataaac gggttgacct
     3841 tactgtccat cataatatca ttgagtacag aaacagcgtt agatgatgct aaataaagca
     3901 tttgagttgt tttatccatc atctcatctt gctttatcct caatgtcttt ttaaccgctg
     3961 caacttttag atacttatga cctgttgcgc gtgatacccc tgctttttga catgctttgt
     4021 ctatcgttgg ctcggtaagc atggcatcta tgaatttaat ttgcttggac gtaaggttat
     4081 cattttcatt tcctgccatc tattacctcc tcattatcaa aataaagggt tgccccttta
     4141 tttccctatg ctagataatt ctgcaattct gcatccattg cctctgaatt gccctcaaca
     4201 atcatttcat gctgtactaa atcaatctta tctccgttaa taagtaaacc accgtggaaa
     4261 taatcaattt ttctatcaag gaaatgtact agcttttcaa ggcgttgctg ttggctgaat
     4321 tgctccatgt caatttcgat ataagcaagg gtagtatcat tatccataat atcttctaat
     4381 tttctaagag ctagaggttt atttttatat ttttctaggt attctctcat ttctgccact
     4441 gttaatttga tactagataa taaacttagt tcagctgcat catctgctgt aataggctct
     4501 tcttttgatt catggtttgc tagttcagca tttttctctt tttctagttg ctgatacaat
     4561 agctgagcag tattttggga atagttttcg ccctcttttt tatattttaa aagttcttgc
     4621 tctgcataca ctttcccgat aatcacttcc ttataaacta attgcccatc ttgagctttt
     4681 agcttaatac tcccatgctc tggaatttca atatacttaa ttataccatt ttttgagtat
     4741 aaaacaaagc ctttctccat catttttaat aatttatcat ccttgttttc agtcatgctt
     4801 ttctccttta tttcatttta ttataatctg aataccccta gtctatttat ttcactaggt
     4861 ttttagggtt cgtatgctaa aatactaccc tttttgtgta ccttatggct gacttttcaa
     4921 attggttagt ttgccacact tggtttatat ggtcgtggaa agcatggcta ttacttctca
     4981 aagggcgatt tctcacccca tgaaaagtgt ctatttttgt ttaggtttgt aagttaattc
     5041 attgtcacat attactcttt aactgattga gtgagtaccg cttatatttg atgccaaacg
     5101 ccttaaaagt gttaccctca agtcctttta gaatacggct ataattccgc tcattgtaaa
     5161 ctatcttaag ctcatcacta tctaggttgg tattaaaaat ggtattttca cgattgttta
     5221 gcacgtcaaa gagtaaatcc tgctcccagt cactcttagg cttaataaca gcattttttg
     5281 ctcctaaatc atcaataatt aagtaatcaa cagacttcat gagttcagta gcttcaaact
     5341 ctgtaagtgt tgcaccttta ccataattcc acccctcttt aatttgtttg atcatttcgg
     5401 ttaggcttac aaaaagcaca ctcttaggtt ctccttttgt cttatacccc tcatttatac
     5461 ctttggcaat agcaactgat aaaagtgttt ttccaatccc tgtacctcct gtgataagcg
     5521 tatttcccct catgccatca agatattttt gtacctgacc ttttgcaaat tctaaaaatc
     5581 gcttttcttc tgatgttaca gcattaaaat catcaaaagt tttagtttta aactcatctg
     5641 ctacatagct cttattgctc atcaacacat tataagtttg catatatagt ttagcattca
     5701 aattatcagc aatcgcatct tcttcatctt gctttttctg ttcttcttgg cattgttcac
     5761 aatagggtgg gatacagcga acttctttta ttgcctctcc gttctcattc caccccacta
     5821 ctacatgtct ttctcctttg atttgtgtta gctgtatttc atgcttagga cacaattcgt
     5881 ctagtttaaa tgtctcaata tttcctaaac tagattgtaa tgatttcatt ttctgacctc
     5941 ctaaaatggt ttttcttgtg ttggtatcca atcttcatag ctggtaggct ctagttgatt
     6001 ggtttgctgt tttttagcct cacgcgctgc cctgctattt ctaacaagtt ccaccgtcaa
     6061 taaattgtcc tgtttccaac ggttaaggat taccttgatg tatgcaaagt ttgctttacc
     6121 ctgactgaca gcctctttta acgcctcatg gataagctct gggctaaaat cttctagcat
     6181 atactgcaat tcttgaatct gtaacggtga caatgcttta cctgtctcag ctcgcttcat
     6241 attcaacaag tcgtctattt ccacactggt tactttttta tttacaaaat cagaaatcag
     6301 ttgaaaaatg tttggacttt gtagctggat ttcagccatt acctcatcaa attctgcttg
     6361 tgtcatgttg tctaaatcta gtgtcattgc attgcctcct caaacttctc tataagacaa
     6421 cttttatttg ctttctgagt tccattttta gagttaaaaa gaatatcttt taaggttaca
     6481 gtagcctcta aatactcctt ttcagcatgc tctatatacg cctgttgctc tgcttcgttc
     6541 tcaaaaaagt gcttagcttg gcgtttaaag aatgcttttc gcatagcgtc catttcaaaa
     6601 ataccagggg cgaaaaacat tcccgtagtg cttttagaga ccgcttcgat tttatggctt
     6661 tcattcaatt caggaagttc aatccaaagt aaacgggaca actcatcttt gatggatttt
     6721 gtctgacttt ccaataaaga aaggattctt aggccatttt cttcgctaat ttctcgcatt
     6781 tctgcgctaa ttctgtctat acgtctagtt aaattctcat atgttgtttc tgtcatgttt
     6841 ttacctctgt ttctttgttg gtgtgatttt ttagcttatt tttttacttc taaacatcat
     6901 tgtcttaatt tcctgataac tcattttcaa ttcaatcata gctattgcca tatcctcaaa
     6961 tgcctggtac tgctccaact cctcactagt caagctatcg ataccgttat agcccccacg
     7021 ctcttctctt aactgcttag cgttcatgtc tgttactgcc tttagtagca agttgttcat
     7081 ggtgctatgc gcgtgctttg gtgcattagg ccatgtttct atactgtcat gcaaggtttt
     7141 tcttttcggt ttttctagcg ccctctgcag acgaatttca gaaagttcct cacgcatttc
     7201 aaagaatgct ttgactaggt ttagtttgaa ttgccgtact gtttcggtat tctttaaata
     7261 agtgatcaga aaagtagcct gttgctcgtt cagaatatag gattttttag gttgtcctct
     7321 agtatctaat ttatggattt taaatccaag tattcccaac tcttcaaagt cagccttatt
     7381 ttctcttatt aagcgcgtga tagtgtggtg ttgtacttca gcacattcag cgatgatctc
     7441 gcttgtggtg tacggctctt tcttaccgtc catgtaaact agttccatta cggttctacc
     7501 tcctgtataa atctggttag cttacttttt aattgcctcc tctagcctct tttttagcct
     7561 ctaaaacggc tttggctagt ggttaatatt atttaccact tgtctctata aacgtgttag
     7621 aggcctttat aacgacttgt atcgctgtat cgatatcctc cgtggaatag tagatttatt
     7681 ttctaatatc attcaagact tgtttaaccc atttcttgaa agaaataaaa ttacatcttc
     7741 tttatccttg gcatctgctt tgtctgagac aaatagaatg tcaatacctg gcagataaaa
     7801 cccatcgtct atttgtttag ttggtttgaa agtaacaatt ttcatttcat aactcctatt
     7861 ttattgctaa aaataaatac acatctgacg gacgatagaa tactttctta gctccatcca
     7921 tcggagtctg atatcgttta agaccaagat cctcccagtt atcaagggtt gtagcactta
     7981 tacctagcag atccattaat tctttacgag aaattaaatc taacttacta gatcgttgtt
     8041 tttctagctc aactaaagtt tcaagagtct gtttaagaag ttgtaagccc tgtaactgta
     8101 tttctttaga cagaatttcc atttatatcc tccatttttc ctttttataa attaagttgt
     8161 cattttcctc taattttgat aaaataagag gagattattt acaaaggggt ttgtcccctt
     8221 gctaaatacc taacaaaacc acgcgccttg cctttgcttg agttttgtta tttttcctta
     8281 gccgtgtacg ttggtttggt cgcctgtaca cggcttttat tttcgcttta attccatttt
     8341 ttgcactctt tacttttgca tacttctgtt taaaaaatat atcttctatt gtaatatctg
     8401 gaaataaagg gattaaaaga tttttaatct taagtttttc tttatctgaa aattgtgtct
     8461 tcccactttc tttgttgtaa taactctgct tagatatacc aagttttttt ccaagtttct
     8521 cttgtgttaa acctagcata ttcctatatc cacgcatctt gttttcttta ctcatttcta
     8581 tacctccttt acttttgcat acctagatta taaaacttta tttttataaa gtcaatactt
     8641 ttctttactt tttcttaaaa aaagtttatt atgttcttta aggaggtgta aaacatgaaa
     8701 ataaataata aactcgttgg agaacgtatt caaaatatcc gtttaagcca tggcgactct
     8761 atggaaaaat ttggagaaaa atttaatact agcaaaggta cagttaacaa ctgggaaaaa
     8821 ggtcgcaatt taccaaataa agaaaaccta ctaaaaattg catctattgg aaaaatgagt
     8881 gttgaagagt tactctacgg cgattacaat acttatctac acttaaagat tatggattta
     8941 gctcctgaat gtataaaaaa ttatgatgag tataactctt tacacgatga tataacaaat
     9001 aaagcgttac agatcgctca aaataccatt tctaagattg attatcaaat ttcagacgaa
     9061 acgatcaaaa aatttattga tttagctatc gaacaatcga gagatttgca aggaaatttg
     9121 ttgaaaaata acttatctaa ttttgactat tcactttttg gaggtactag aaaccgagaa
     9181 tatttctatt ttgggtatag acttacctat aaagaaaaat atctatacac tgccttacta
     9241 gctatactga acgagtcaac aaaaacgata tatattgatg atataaatac agcaagattt
     9301 tttaacttct ttggcaatga tattcctaat tcgtctttaa aaaaaattga ctatatcgca
     9361 ccttcagaaa ttgtttcatt tagtacgtac gttcgacaac gttctaaagt aattcctaaa
     9421 attttggaac atatattaaa atcaagtttt ttattagaga atatagatgt ttctggttac
     9481 actgtaaata ttttagaaga tcaattaaca aaacatagaa caatcaaaat tagtaaaaac
     9541 taactggttg atctcatgta taaataccta acaaaaccac gcgccttgcc tgctgatgga
     9601 aagaaaggta caaatacatg aatatcaaag aaaaaatcaa aaagaatggc caaagagttt
     9661 attatgctag tgtttatcta ggcgttgacc aactaacggg caaaaaagcc cgtacaactg
     9721 ttacagcaac cactaaaaag ggcgttaaag taaaagcgcg tgatgcgatc aatacttttg
     9781 ctgctaatgg ctatacagtt aaagacaagc cgacaattac aacatataat gagcttgtaa
     9841 aagtttggtg ggatagttac aagaatacag ttaagccaaa tactcgccaa tccatggagg
     9901 gattggttag agtgcattta ttgcctgtat ttggcgatta caagctatct aaacttacta
     9961 cgcctattct tcaacagcaa gtaaacaaat gggctgacaa ggcaaataaa ggcgaaaaag
    10021 gggcatttgc taactactct ttgctccata acatgaataa gcgtattttg aaatatggcg
    10081 tagctatcca ggtaatacaa tacaacccag ctaatgatgt catcgttcca cgcaaacagc
    10141 aaaaagaaaa ggctgctgtc aaatacttag acaacaaaga attaaaacag tttcttgatt
    10201 atttagatgc tctggatcaa tcaaattatg agaacttatt tgatgttgtt ctgtataaga
    10261 ctttattggc cactggttgc cgtattagtg aggctctggc tcttgaatgg tctgatattg
    10321 acctagaaag cggtgttatc agcatcaata agacactaaa ccgctatcag gaaataaact
    10381 cacctaaatc aagcgctggt tatcgtgata taccaataga caaagccaca ttacttttac
    10441 tgaaacaata caaaaaccgt caacaaattc agtcttggaa attaggccga tctgaaacag
    10501 ttgtattctc tgtatttacg gagaaatatg cttatgcttg taacttacgc aaacgcctaa
    10561 ataagcattt tgatgctgct ggagtaacta acgtatcatt tcatggtttc cgccatacac
    10621 atactactat gatgctctat gctcaggtta gcccgaaaga tgttcagtat agattaggcc
    10681 actctaattt aatgatcact gaaaatactt actggcatac taaccaagag aatgcaaaaa
    10741 aagccgtctc aaattatgaa acagctatca acaatttata aaaaataagg gtgacccatt
    10801 tccgggctac cctcttacta taccaaaaat tagtaggggt agtaaaaagg gtattaaatt
    10861 ataaaaagca ctaagggaaa gcgccccaaa gtgcttattt caaaggcttt atagcctata
    10921 atcacataaa gagattattt tttaaggttg tagaatgatt tcaatccacg atattcagct
    10981 acttcaccaa gttggtcttc gatacgaagc aattggttgt atttagcgat gcggtctgta
    11041 cgtgaaagtg aaccagtctt gatttgtcct gcgttagttg caactgcaat atcagcgatt
    11101 gttgaatctt cagtttcacc tgaacggtgt gatacaacag cagtgtaacc agcttcttta
    11161 gccatttcga tagcttcaaa agtttcagta agagtaccga tttggttaac tttgataagg
    11221 attgagttag cagcaccttc ttggatacca cgtgcaaggt agtcagtgtt tgttacgaag
    11281 aagtcgtcac caacaagttg tactttctta ccaagacgtt cagtaagagc tttccaacca
    11341 tcccagtcgt tttcatccat accatcttca atagtgatga ttgggtattt gttaaccaat
    11401 tcttcaaggt agtcgatttg ttctgcagat gtacgaacag cagcaccttc accttcaaat
    11461 ttagtgtagt cgtaaacttt acgttcttta tcgtagaatt ctgatgaagc acagtcaaat
    11521 ccgataaata cgtctttacc tggtacatat ccagcagctt caatcgcagc aaggatagtt
    11581 tcaacaccat cttcagttcc ttcgaaacga ggagcgaatc caccttcgtc acctacggca
    11641 gtttccaaac cacgtgattt aaggattttc ttaagagcgt ggaagatttc agcaccgtaa
    11701 cgaagggctt ctttaaatgt tggcgcacca actggcaaga tcatgaactc ttggaaagcg
    11761 attggagcgt cagagtgaga accaccgttg atgatgttca tcattggagt tggaagaact
    11821 ttagtgttga atccaccaag atagctgtaa agtgggattt caaggtagtc agcagcagca
    11881 cgagctacag cgatagacac accgaggatt gcattcgcac ccaatttacc tttgttagga
    11941 gtaccgtcaa gtgcgatcat agcacggtca atagcttgtt gatcacgtac atcgtagcca
    12001 atgatagctt cagcaatgat gttgtttacg ttgtcaacag ctttttgtgt accaagacca
    12061 ccgtaacgag atttgtcacc gtcgcgaagt tcaactgctt cgtgttcacc agtagaagct
    12121 cctgatggaa ccataccacg tccgaaagca cctgattcag tgtaaacttc tacttcaagt
    12181 gttgggttac cgcgtgagtc taggacttcg cgagcgtaaa catcagtaat aattgacatt
    12241 ttttactctc cttatgagtt aaatttttta cacctctata ataccttaaa acccctcctt
    12301 tttcaagaaa aaacgttatc tttgtgcaac ttttccttaa ctttataaag taatcgcttt
    12361 cttttgtctg ttttattcta acttttatga tatactgttt tcatgacaga tttatcaaaa
    12421 caattacttg aaaaagctca tggtgggtta aaaataaatc cggatgagca aagacgctat
    12481 cttggtactt ttgaggaaag agttcttgga tatgtagata ttgacacagc aaatagccct
    12541 cagttagaaa aaggcttttt atttatttta gaaaaccttc aggaaaaagc agagccacta
    12601 tttgtgaaga tttcaccaac tatcgaattt gataagcaag ttttctactt aaaagaagca
    12661 aaagaaactg atagtcaagc caccatagta tctgaagagc atattacttc tccttttggc
    12721 ctggttattc atagcaatgc accagttcaa gtagaagaaa aagaccttcg acttgctttt
    12781 ccaaaacttt gggaagttaa aaaggaagaa ccagccaaaa catccttatg gaagaaatgg
    12841 tttagctaaa tcttgcacat atttaataag tgcccaatat tggcagccgt gcgctccaga
    12901 tagaaactgg catttttcaa actatcttct aaaggttcac ttttctccaa aatagaaaag
    12961 acagcttgga tattttcaaa tggtagggga ggtaaatctt cagcaagact accgcaaata
    13021 gcaataacag gaactccaac aggggttctt tttgcaacac ctataggcgc tttcccagca
    13081 aagctttgac tatcaagtct tccttctcca acaacaacca agtcagcatc tgaaactttc
    13141 ttatcaaagt tgattaagtc caagcaggta tcaattccag acacgatact tgcctgagca
    13201 aaggcacaca aaccaccagc aaggcctcca cctgctcctg ctcctttaat ttctaatgtt
    13261 gcaggtgaga atttttcata aaaatcttgg atcgcctgat ctacgactgc aaacatagtc
    13321 ggatgtagac ctttttgatt gccaaaagtg taagtcgcac cttgatgacc acataaggga
    13381 ctcacgacat ctgctaaaat atgaatttga acaccttcag gaattttata gcaattttct
    13441 gttgaaacag aagctaagtt taataaggat tgaccggaag caggcaagac atttccatcc
    13501 ctatcataaa attgataacc taaaccagca gcaatcccca gtcctccatc attactggcc
    13561 gtgccaccaa caccgatata aatatcttta atccctttag agatgagatg aagaatcaac
    13621 tctccaatac cacaagtttg gatttgaagt ggatttcgtt tctctagcgg aatttttcca
    13681 agaccaacca agtcagctac ttcaaatagt gccagttccc ctttttgaaa atagcgcatg
    13741 gcttcttttt gtccaaaagg gtctgtcact tggatccatt tttcttttag gtcaagagaa
    13801 tgtcggatag catctacagt accttctccc ccatcaccaa cagggcagag gagacattct
    13861 acatctgcta tcgattgttg gaagcctctt tttattgctt cagctacctg ttgagctgtc
    13921 aagctttcct taaacgaatc cggtgcaatt acaatcttca tattttccct cattctaaac
    13981 agtcaatcaa agggagaact tctaaaaaat ccctcttgtc aacatgatgt ggtatttctt
    14041 ttttgagcac ttctttggca caaaaggcga ttcctaactt cgccgacttc aacattaata
    14101 gattattaac cccatcaccg attgccaccg ttctttcttt agaaagtttt agtttctttc
    14161 tccatttttc cagagtctct tttttgacct ggggacttat aatttgtcca actaattttc
    14221 ctgttaaaag accttctttg acttcaagct agttggcagt gaaataggca ataccaaggg
    14281 attttgctaa tctctccaac tattggtgta aatccaccag acaccagacc aactaggatg
    14341 ccattctttt ggagaataga gatgaactct gggacattta gcgatagatg aattgagttg
    14401 aagacgttat caaagaccaa aataggaaga ccttccaaca aggacactct ttttcttaaa
    14461 ctgctttcaa agaccaactc tcctcgcatt gctcgacttg taatctgcga aatttccgcc
    14521 tcatgacctg cctctctccc taaaagatca atcacttctt ctaggattaa ggttccatct
    14581 acatccaaaa cacacaagcc ttttacttga gacatcagtt ctcctctcta aacagcctaa
    14641 aaatcgtatg aagtcatcat acgattttat ctattaatta actaaactat ggtacaagtc
    14701 aaggtatgac ttgcaggctg tatcccatga gaagtcactc tccatagctt gtttttgtag
    14761 gtttctccaa atgtctggat ggtttctata caagtccaat gctgtttgga aagtccaatt
    14821 taaccaataa ggagatagat tgtcaaagct aaagccagta ccgcttcctt cgattggatt
    14881 gaaagcgcga actgtatctc gcaagcctcc aacttcatgg accaatggca aggttccata
    14941 acgcatagcc atcatttgag acaagccaca cggttcaaaa cgacttggca tgaggaagag
    15001 gtcacaagca gcgtagattt cctgagcaag tttgacatca aaagtgatat ttgttgatag
    15061 cttgtctggg taaatctgag caaaccatga gaaagctcct tcaaaggctg gatcgccagt
    15121 tcccaaaaga acaatctgaa catcttcttg caagatatgg tgaagacttt cgaccaccac
    15181 atcaaaacct ttttgacgtg tcaaacgaga aacaattccc accagtggaa cgtctgctct
    15241 aacaggcaag ccaactcttt cttgcaattt tgccttattt ttggctttcc cagacaaatc
    15301 ttcctgattg aaatgatagt ctaaaagagc atccgtctga ggattataaa gatcagcatc
    15361 aatcccattc acgataccag atactttacc agactccatt ttaagaatct gatccaaatt
    15421 acatccaaac tgactagtca taatttcatg agcatagcta ggtgaaacgg ttgaaacacg
    15481 gttcgcatag agaatacctg ccttcatcca gttcagacag ttgttccatc gaagggtgcc
    15541 atcagcgtaa cgttcaaagc caactccaaa caaatcaccc aacattcctt ctgaaaattg
    15601 tccttggaat tctaaattat gaatggttaa aactgtttca atgtcctcat aggcttgaat
    15661 ccaacggtat ttttccttca acaagaaagg aatcatagct gtatggtagt catgaacatg
    15721 gagaagatca ggaataaagt caatcctttc catagcctca atggcagcca gttggaaaaa
    15781 ggcaaagcgt tctccgtcat caaaatcacc gtaaacatga ccacggaaga aataatattg
    15841 attgtcaata aagtagaagg ttacaccatt taatactgtt ttcttaattc cacaatactg
    15901 tctgcgccaa ccaacgctca cctcaaaatg aagcacatct tcaatctgat ttccaaattt
    15961 agcctctacc atatcatagt agggtaaaat cactgcaact tcgtgcccag cttttaccag
    16021 tgattttgga agagcgccaa tgacgtctcc caaaccacct gtttttgaaa agggtgcacc
    16081 ctctgctgct acaaataaaa ttttcatgaa tgaatatcct ctgttacttt agcacctttc
    16141 ttaaccacaa ctggatgttc tgcagttcct cgaatcacaa caccatgctc aacttcaacc
    16201 cctttgtcca agatagcata ttcgacctga gccccttctc caataacaac acgagggaag
    16261 agcaggctat ctttaaccaa gctatcctta tggacatgaa tattacgtga tagaacagaa
    16321 ttagctactt gaccttcaat aatactacca gaggcaaact gagaagtgct taccttagat
    16381 gtattagcat agtaagttgg ctcttcgttt ttgacctttg tataaatctt ttggtttggt
    16441 gagaaaagag aatagaattt ttgtgattca agcatatcga tattcgcttg ataataagat
    16501 ttaacagagt gaatattggc tagatagccc gtgtactcgt aggcgaaagc tccctctttt
    16561 acagccaaat cccgtaaaac atagcgcaat ttctctggat gttctttttt agcttcttct
    16621 tccaagtgtt caatcaacca aggtgtatca acgacaaaga tatctgtaga catattgaac
    16681 gtttcagctg ttgacttgct atcaaagagt ttatgagaaa gaacatggtc tgtttcatct
    16741 acatccaaga ttgcatttac ttctgaaata tctttcttag ctagtttttt ataaactaca
    16801 gtgataggct cttttgttgt actatgtagg tggaaaactt ggttcaaatc aatgttaata
    16861 agaacatcgc agttgagggc aaccgtttgg tttgagccag aacgtttcaa ataagtaaga
    16921 agctgttggt agtattcttt tccaactgta ctactttcta cacgggtatt gtaaattcct
    16981 agatagtaat ggctaagaag ggttgataag ccccactcgc gtcctgaacg aatatggtca
    17041 aatactgagc tgatattatc ctgctggaaa ataccaaaga cactacgaac acctgcatta
    17101 gcaaggcttg aaagtgggaa gtcaatcaaa cgatatttcc caccaaatgg caaacttgct
    17161 actggacggt ggtccgtcaa tgtcgacata ttgtgaaaac caactgtatt tcctaaaatg
    17221 gcagaatatt tatcaatctt catctgttgc tacccccact acttcattat atcctacaac
    17281 ttgtacttca tctgttccat caatttcgac accgtcagaa ataatcgcac cttcaccaat
    17341 aatggcacgt ttaatcttag ctccttgacc aatgatagct ccactcatga taactgaatc
    17401 aaggacttcc gctccttcgc gaacttgcgc gcctgttgaa aggatagaat gtttaacagt
    17461 tccatcaacg aaacatccgt ctacaactaa tgagtcttcc acatgagcat ttgccccgag
    17521 gaagtttggt ggtgaaatca agtttcttga gtaaatcttc cattgacggt tacgactatc
    17581 caaggcattt tctggagaaa tatactccat gttcgcttcc caaagtgact caatagtacc
    17641 aacatctttc caataaccac taaattcgta agcataaaca ctttcacctg actcaaggta
    17701 atttggaatg acatttttac caaagtctga catgccaacc ttgctctttt cagcagcgac
    17761 taacatatta cgaaggcgtt gccaatcaaa aatgtagatt cccatagaag cttttgtaga
    17821 tttaggttga gctggttttt cttcaaattc aacaatacga ttgttagcat ctgtgttcat
    17881 gataccaaaa cggcttgctt ctttaagagg gacgtctaaa actgctactg tcaagctggc
    17941 attattatcc ttatgagact ggagcatatc atcatagtcc attttgtaga tgtgatcccc
    18001 agacaaaatc aagacatact caggattgac actgtcgata tagtcgatat tttggtaaat
    18061 agcgtgacta gtcccctcaa accaacgatt tccttcactt gcagaataag gttgaagaat
    18121 agagacacct gaattaatac cgtctagtcc ccagcttgaa ccattcccaa tatggttgtt
    18181 gagagcaagt ggttgatact gtgtaacgac cccaacattg tgaatccctg agttggcaca
    18241 gtttgatagg gcaaagtcaa tgatacggta gcgcccacca aattgcacag ctggttttgc
    18301 gatgctttga gtgagtttac cgagacgagt tccttgccca ccagcaagaa tcaaagctaa
    18361 catttcattt ttcattttct actccttttt ggtttttatt tgtgacggtt ttagtagatt
    18421 tcaagcgacg tttgattttc catacacttg ctcccatagc cggtagggta aaggttaagg
    18481 tctgctcata atctttccat agtccttctt gcgtttgaac agtttgatta tgttctttcc
    18541 aaacgcctcc ccactcttcc aactcagtat tccatacttc ttcgtaaatt cctgcaacgg
    18601 gtagtccgat tgtaaaatct ttccgctcaa caggtaccat attaaagata cagactaaca
    18661 tttctccctt tttaccctta cgaataaagg aaagaacact ctggtctcga ttatccgcat
    18721 caatgatttc aataccatca tagctggtat caatttccca cagacagcga tgatctttgt
    18781 aaaactggtt tagctgagaa gcgaaatact tcatcttagc attcattggg tcttctaggt
    18841 tagaccattc caactgttct tcagatttcc attctaggaa ttgaccgtat tcgctaccca
    18901 tgaagagcaa tttcttacca gggtgacaaa tttggtacgt atagagattg cgcaagcctg
    18961 cgaattgatt gtaacgatct ccccacatct tatgcatcat actcttcttg ccatgaacca
    19021 cttcatcgtg cgagaatggc aagagataat tctccttgaa aacatacata aagctgaaag
    19081 tcaccaggtt aaagtcatat ttacgataga tcggatcttc ttcgtagaaa cggaggatat
    19141 cattcatcca gcccatgttc catttgtagt caaatcctag accaccaatc tctttcattc
    19201 ccgtaatctt gatcgcagac gaactttctt ctgcaatcat catcacatct ggatattcta
    19261 acttaataac ctcattcaag cgctgaagga aataataacc ttcatagttg agatttccgc
    19321 catctttatt aggtgtccat ggagcatcat catagtccaa atagagcatg ttgctaacag
    19381 catccacacg aataccatcc aaatgataga catcaatcca atgcttaatg caagaaatta
    19441 agaaggactg gacttcattt tttccaaggt caaaattaag ggcaccccaa ccatggttat
    19501 gagccttatt atggtcttgg tattcaaaag tcggtgtccc atcataatag gctaaggcat
    19561 catcgttgat ggtaaagtga cctggtaccc agtccacaat aaccccaata ttatgggtat
    19621 gacactcctc gacaaaatct tgaaactcct ctggtcggcc ataagcatgc tctaaagcga
    19681 agtaacccat aagctgatac ccccaactca agcccaaagg atgggacatc aagggcataa
    19741 actcaatatg agtatagttc atttcaacga gataaggaat gagttcatcc ttgagctggg
    19801 caaaactata aggactgcca tcagaatttc ttttccatga tccagcgtga acttcataaa
    19861 tattgacagg acgctcttca aagccccaac gttttcttcg tgccagccaa agtccatcct
    19921 tccatttctt ctcaggaagc tctgttacga ttgcccctgt tcctggacga gcctcatacc
    19981 tgacagcaaa agggtcaatc ttcatcagtt gatgaccatt ttgacgtgtg acatgatatt
    20041 tgtaaatatg cccttcttga gccatattgg taaagacttc ccagacccca aaatcatttc
    20101 ttaccattgg aatctgattt tcaatccagt tggtaaaatc accaaccaag tgaacagcct
    20161 gagcattagg tgcccaaaca cggaaggtat agccatgctc tccatttagt tcttccctat
    20221 gtgctcctag ataatgttgg agataaaaat tttcacccgt cataaaggtt tttaatgctt
    20281 ctctattatc catatactcc ccttctcctg taagcgtttt ctatgttttt attatactac
    20341 ctttttagag aagattcaag taaattacta tacttcttta attattttga aaatctacaa
    20401 caagttcact tactcgttca attgtaaatc aatatttttt caaaaaattg cgaaaacgcc
    20461 tttctttttc tactatagtg aaatgaaata aaacatgcgc aaatcgatta aggaatttaa
    20521 tctaatttct aacaatgtct tagaaatcaa agtgtactat tttaactcca atgtattata
    20581 tatagtaaaa tgaaataata acaggacaaa tcgatcagga cagtcaaatc gatttctaac
    20641 aatgttttag aagtagaggt gtactattct ggtttcaatc tattataata ccaaaaagtt
    20701 cgtatttttt ctataattat tattatttta tagcaaaaaa ataaaaccag gaaaacaatt
    20761 tcctggtttt acacgttatt tcacatcaaa aacaatggat ttgacatttg tcatcgcttc
    20821 aatgctatat ttaattcctt gcactccagc tccagaacct ttgacaccaa ggaatgggaa
    20881 attatctgga ccacgctggg ttttattatt aatgtggact gtacctactt caagtttttc
    20941 agcaatttca aaggcttttt tgaaatcatt tgtaaagact gatgattgaa ggccgaattc
    21001 agattcgttg gcaaaggcaa tagcttcctc tacactagcc acacgaatga ttggtaaaac
    21061 aggaccaaat ggctcttccc atgccacttt catatctttt gtaacttggt caaaaagcac
    21121 tggccagaga agattgccct cacgtttgat tggtgtaaga gcctgagctt ctttttcttg
    21181 tgcatcctca atcaagcccc aaatgaagtc ggctgaagca ttgtcaataa caggtgtaat
    21241 atcagcattg tcaaatggat caccaactgt taatttagaa acttcttcct gaagcaaagt
    21301 agctaattta tctgctacac tttcgagaac aatgacacgt ttaatggccg tgcaacgttg
    21361 tcctgagtag ctaaaggctc ccgcaacaat ttgcttggca gcatgttcca aatctgcatc
    21421 ttctagtaca agagctgcat ctttcccacc aagttccaac atgataggac gcataccagc
    21481 taaacgacca atacgttctc caataggagt tga
//
LOCUS       DBDESG010000041        21276 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.041, whole genome shotgun sequence.
ACCESSION   DBDESG010000041 DBDESG010000000
VERSION     DBDESG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.041"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..427
                     /locus_tag="ACKB38_001444"
     CDS             <1..427
                     /locus_tag="ACKB38_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980201.1"
                     /translation="LANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNYCRDMKLFKMS
                     RRNIGQAGKILSDSGYQGLMKIYPQVQTPRKSSKLKPLTAEDKACNHALSKERSKIEN
                     IFAKVKTFKMFSTIYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(419..1062)
                     /locus_tag="ACKB38_001445"
                     /pseudo
     CDS             complement(419..1062)
                     /locus_tag="ACKB38_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(1171..1689)
                     /locus_tag="ACKB38_001446"
     CDS             complement(1171..1689)
                     /locus_tag="ACKB38_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HGL7980202.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1726..1854)
                     /locus_tag="ACKB38_001447"
                     /pseudo
     CDS             complement(1726..1854)
                     /locus_tag="ACKB38_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            complement(1916..2785)
                     /gene="rsmI"
                     /locus_tag="ACKB38_001448"
     CDS             complement(1916..2785)
                     /gene="rsmI"
                     /locus_tag="ACKB38_001448"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGL7980203.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2788..3105)
                     /gene="yabA"
                     /locus_tag="ACKB38_001449"
     CDS             complement(2788..3105)
                     /gene="yabA"
                     /locus_tag="ACKB38_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGL7980204.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(3145..4035)
                     /locus_tag="ACKB38_001450"
     CDS             complement(3145..4035)
                     /locus_tag="ACKB38_001450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGL7980205.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFESLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(4032..4670)
                     /gene="tmk"
                     /locus_tag="ACKB38_001451"
     CDS             complement(4032..4670)
                     /gene="tmk"
                     /locus_tag="ACKB38_001451"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGL7980206.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4786..5583)
                     /gene="proC"
                     /locus_tag="ACKB38_001452"
     CDS             complement(4786..5583)
                     /gene="proC"
                     /locus_tag="ACKB38_001452"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGL7980207.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(5587..6849)
                     /locus_tag="ACKB38_001453"
     CDS             complement(5587..6849)
                     /locus_tag="ACKB38_001453"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGL7980208.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(6859..7968)
                     /gene="proB"
                     /locus_tag="ACKB38_001454"
     CDS             complement(6859..7968)
                     /gene="proB"
                     /locus_tag="ACKB38_001454"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGL7980209.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQYGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(8069..9952)
                     /gene="cbpE"
                     /locus_tag="ACKB38_001455"
                     /gene_synonym="pce"
     CDS             complement(8069..9952)
                     /gene="cbpE"
                     /locus_tag="ACKB38_001455"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGL7980210.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9955..10842)
                     /locus_tag="ACKB38_001456"
     CDS             complement(9955..10842)
                     /locus_tag="ACKB38_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGL7980211.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10832..11293)
                     /gene="lspA"
                     /locus_tag="ACKB38_001457"
     CDS             complement(10832..11293)
                     /gene="lspA"
                     /locus_tag="ACKB38_001457"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGL7980212.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(11290..12198)
                     /locus_tag="ACKB38_001458"
     CDS             complement(11290..12198)
                     /locus_tag="ACKB38_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGL7980213.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKSQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            12191..12388
                     /locus_tag="ACKB38_001459"
     CDS             12191..12388
                     /locus_tag="ACKB38_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGL7980214.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSSQPY
                     FQNQFKKITGYTPLQYRKEKR"
     gene            complement(12440..13111)
                     /locus_tag="ACKB38_001460"
     CDS             complement(12440..13111)
                     /locus_tag="ACKB38_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGL7980215.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(13165..13386)
                     /locus_tag="ACKB38_001461"
     CDS             complement(13165..13386)
                     /locus_tag="ACKB38_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGL7980216.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLL
                     VHGFCPRGTYDSGYIGGGNHLCKGSAARF"
     gene            13658..14467
                     /locus_tag="ACKB38_001462"
     CDS             13658..14467
                     /locus_tag="ACKB38_001462"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGL7980217.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGDKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYIKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14648..15523)
                     /gene="aguB"
                     /locus_tag="ACKB38_001463"
     CDS             complement(14648..15523)
                     /gene="aguB"
                     /locus_tag="ACKB38_001463"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGL7980218.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15533..16618)
                     /gene="aguA"
                     /locus_tag="ACKB38_001464"
     CDS             complement(15533..16618)
                     /gene="aguA"
                     /locus_tag="ACKB38_001464"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGL7980219.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16615..17742)
                     /gene="nspC"
                     /locus_tag="ACKB38_001465"
     CDS             complement(16615..17742)
                     /gene="nspC"
                     /locus_tag="ACKB38_001465"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGL7980220.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17742..19001)
                     /locus_tag="ACKB38_001466"
     CDS             complement(17742..19001)
                     /locus_tag="ACKB38_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGL7980221.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18998..19858)
                     /gene="speE"
                     /locus_tag="ACKB38_001467"
     CDS             complement(18998..19858)
                     /gene="speE"
                     /locus_tag="ACKB38_001467"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGL7980222.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            20114..20719
                     /locus_tag="ACKB38_001468"
                     /pseudo
     CDS             20114..20719
                     /locus_tag="ACKB38_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            20699..20824
                     /locus_tag="ACKB38_001469"
                     /pseudo
     CDS             20699..20824
                     /locus_tag="ACKB38_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20796..>21276)
                     /locus_tag="ACKB38_001470"
     CDS             complement(20796..>21276)
                     /locus_tag="ACKB38_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGL7980223.1"
                     /translation="IGLTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERL
                     VGALADIKRLYSRDGKDLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVM
                     CYPPGIPILAPGERITREIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         6208 a         4894 c         3787 g         6387 t
ORIGIN      
        1 attagcgaat cattctggta aaaagaaatt tcacgctatg aaggctcaag cgattgtcac
       61 aagtcaaggg agaattgttt ctttggatat cgctgtgaac tattgtcgtg atatgaagtt
      121 gttcaaaatg agtcgcagaa atatcggaca agctggtaaa atcttgtctg acagtggtta
      181 tcaagggctc atgaagatat atcctcaagt acaaactcca cgtaaatcca gcaaactcaa
      241 gccgctaaca gctgaagata aagcctgtaa ccatgcgcta tccaaggaga gaagcaagat
      301 tgagaacatc tttgccaaag taaaaacgtt taaaatgttt tcaacaatct atcgaaatca
      361 tcgtaaacgc ttcggattac gaatgaattt gattgctggc attatcaatc atgaactagg
      421 attctagttt tgcaggaagt ctaatgtaat tctcaagctg tgagttcatt atgatttgta
      481 aatccgatat atcttcagca gctaaaaaca cattttgaaa tgttccaagt ccgttttcac
      541 caaaactata ttctctctta ctatacttac tcttattatt ctctatatag ttagagtcaa
      601 ggttcttaac ctcttgaagt tcattttctt tacttctgag gttaaaatct tttacttctg
      661 aagttaagtt ttttgacttc tgtaagtcat tttcttttac ctgaaatata ctcataaagt
      721 ctttaacata aatgatgttc ggcttaccaa gtccaagcct tactctttcg atcagtccta
      781 ttcctttttt acatcaagct catctaatgt ttttatggca gttggctttg agatatttct
      841 tcttttcata atttcttcga cagtaaaata gataaatact ctgccttcct tgtctatcca
      901 gttattctta aatgacattc ctgtctgttt aagaagtatg gaataaagga taattgcttc
      961 tgctgataag ccccgaaatt cttctctatc aactaatatc tccggtactt ttaagaagtt
     1021 aaatctttcc gcttctctgt tataaaaata ttcaaagtcc atgcaagatc acctcccttc
     1081 ttaaaaattt gcaaagaaaa agacgatagt ttttcttcta tcgcctttgg taaccatttt
     1141 tatgaaattt tttaaagtta aaaatataat ttataatact atcttcttcc attttctgat
     1201 tttagttctg aaagttccaa aaggtgcaac cgtatttaca tgaataaact tatacacttc
     1261 ccaagtcgct gttttagtcg cttcatcagc ccactttctc atatgcggtt caaataattc
     1321 ttcctcactc aacgaatcaa tcattgcaga gatagaatta atattttcat ttaattttgc
     1381 tttcaactct tgcagagata aatgagcgta ggtatctgtg aaccactgat ataattcacc
     1441 aagttgattc catttaaatt tatccgatgg tgtttttact tgaagcccct ttctttcatc
     1501 ttcttcccat ttaagaacca agttggtcca accaacctga taagcaaggt tttctgctgg
     1561 agttctgtca acttcatcag ctctcttatc ttttaaattt tctggaatat tatcaaattc
     1621 taaaatatat ttctcaaatg ttttctctat ctcagctttt agttcttctt tattttcgta
     1681 tgttcgcaaa aaatcacctt ctttctgatt tatcaatcct ttgatttaaa atgaccttat
     1741 ttttctattt ataccttcta agaacgctat aactgctgct ccaaccatcg gtattccgta
     1801 tgggcttaat aaaaaactta ataccaaagc ttctatacca agtgatacat ctttatatta
     1861 tcatcactaa ttaagttaaa cagaaacaga cagaattatt acatcctgtc tccctttatt
     1921 gtttttcttc ccagtcgtgg taggcagcgt agagctgact tttattccac tggtaaatct
     1981 tagcgacttc cttgatagct tggtttttct tcacaccttg ctggatgcgg gtttgaattt
     2041 ctacgaacaa gtcttcctcg tccttttcct ccacaccctg actggcaccc tcaacaatga
     2101 gaagacattc gcccttgagt ggcgtttcag caatgctttc taataactca gagatagtac
     2161 ctcgttggta ttcttcatag attttggtca attccctgac caagacaacg gagcggtcac
     2221 cgtagacttc taacatattt tccaacgtgt ctgctacacg atgaggtgat tcataaaaaa
     2281 tctgtgtttc aggataatct tttttcaagc caaaaaattg cttctgctga cctgattttc
     2341 tcggtaaaaa accgtaaaag atatgtggct gtggcgctaa accactggca atcaaggcag
     2401 aaattcctgc agaggcacct ggaactgtca caactgcaat ttcttcctca atagctgcct
     2461 taactaaatc atgaccaggg tctgaaatgc taggcaaacc ggcatcagag acctgagcaa
     2521 tactttgccc tgctttcaag aaaccaatca aatcaggaat tttttccttg gcattgtgct
     2581 catgaaaact gatctgcttg gtggaaatgt caaaatgctt gagcaaaagc cctgtattgc
     2641 gcgtatcctc agcagcaatc cagtccactt ctttcaaggt ctggatagca cgaaaagtca
     2701 tatcatctag attgccaatc ggcgttgcca ctagatacag cttgccatag ggagactgcc
     2761 ccttaaaact tttttgaatc tgcatgccta ctccctgtat agcaactcgt cacaaaacat
     2821 acattcctcg tcctgctctc gacgttgtcc ataaaaatca ttacatacgt gaaatccatc
     2881 acggtaaatg cgacggacac tttcacgaac atgcttggcc ttgacaggag catctgcttc
     2941 cacctcaccc aagcgttctc gcaacttact attttccaag cgaagagctg tattttcctc
     3001 taccaggctc ttgagatttt tcttgatggc ttccacatcg gctaaggtta ccaataattg
     3061 ttgggaaaaa tcatccagcg cgtcaaataa ttcttttttg tccataaaac agccctttcc
     3121 tttctttatc attcattttt gagtttatat ttctttcaag accagatatt ccatggcatt
     3181 ttgaaagctg acattagctt gccacatttt tctagcttct agtaaatctt gtagaatcac
     3241 tcttactctt acctgcaaga ggtcctgccc acagagaact tcaagaatcc gtaaaacctg
     3301 atcctgtttt tccttatcat ctgccaagtt ggctaatttg gcaacctgta gataactttc
     3361 ttttttctta gctactaacc aagtcagcag gcgttcactt tcatcgacca aggtccaaaa
     3421 acttgcctga ttagccaact tttctgcttc agctcgcgat tgactaaact tagctaaaag
     3481 agtcgctttt ttcttaacaa gtcccatttg ttctaagagt aagataagtt tttcttcttg
     3541 ctttttaaag tggaagatct gagtccgact tcggattgtc ggtaacatct tttcctcatc
     3601 gctagtcaag aagaaaatat aaacttcact ctggggttct tcgatgacct tgagcagaga
     3661 attggctgcg ttgggatgca ttttatccgc ttgctcgatg ataaagacct gttgctggct
     3721 ttcaatccct gcttgagaaa actgacccac caattctcga atgcgttccg tcttaatgac
     3781 ctgattaact ggtttaatca aggtgacatc gggaaattct ccctgttcaa tcagcttgca
     3841 acttcggcat ttctcacatg gtaagacgcc aactttatcc gtacaaaaga ggctcttagc
     3901 taaaaattgc gccatttcca agctttcaaa gaaacctgaa aagagatagg cgtgattgag
     3961 ctggtcttgt tctaagatac ggacaaaacg gtcaaactga gctggttgcc aagcctttag
     4021 ttgatcttgt ttcatttggc caagcccatt ccgtcaaaca agacagcctt ggtagtttcc
     4081 acaacttgct ccaaagggag actagcatca atcttgacaa tgcgatttcc ctctttatcc
     4141 agaagagaaa ggtagccttg acgaactttt ttatgcaagt ccaacccttc caaatccaaa
     4201 cgattaacct cgcggtcact attagcagca atacgagcca gcccttcttc cacctcgatg
     4261 tcaaaataga gtgtcaaatc gggtttgagg ccatctgtcg caaactgatt gagccagtca
     4321 atggcttcaa tatctaagcc acgaccaaat ccctgatagg caacagaact atcgataaaa
     4381 cgatccatga tgaccaactt gccagcttca agggctggaa gaactttttc caccaaatgc
     4441 tgtctgcgac tggcaatata gagaagtagc tctgttttag catccatctg agtatgactt
     4501 ggatccaaaa tcacttcccg aatcttctcc ccaatcaaga ctccgccagg ttcacgggtc
     4561 gtcaacacct ctactccttt ttcctctaaa attggtagca gagcctctaa aacactggtc
     4621 ttgcctgctc cctctggtcc ctcaagagag actaaaaatc cttttgacat gtctaactca
     4681 tttctttttt actacttcta ttctatcaaa aaaatagggg tttgtgacaa tctttttgtg
     4741 tcttctcatt tcagccacca taaaagaggc agtcaaaact acctcttatt tacctagttc
     4801 ttgtgttcgt ttgtaggctt gatgaactgc atccatgact gttcctcgga aagcatgcgc
     4861 ttctaggctt gctacaccag cgatagtcga accgcctggg ctacagactt ggtctttcaa
     4921 tactccagga tgttgctgac tttcaaggac caattgccca gctcctacca cagtttgtgc
     4981 tgccattttc aatgctattt ctcgtggtaa tcctgtctga acacctgcat ctgccaaggc
     5041 ctcgataaaa agatagacaa aggctggtcc acaacctgca agacctgtcg ctgcatcgat
     5101 taaactttct cctagttcaa ccaagagacc agccttggct aaaagctgat aaaagagctc
     5161 actgtcctca gccctgcaat taggagacaa ggcataacta atcactcctt gcccgataga
     5221 agcaggggta ttaggcatca tacgaataat tcggtgttga cttgggataa gacttgctag
     5281 tttttctaag gtcaatccag ctgccatcga aatcaaaaga agactttctc ttttttcaag
     5341 gatggtctgg tattgagaaa gcagttcaga aaactgagca ggcttaactc ctagaaaaat
     5401 cacatctgct tctgcaaaca tttcttcatt gctggaagcc tgaccaccaa agtctgcaat
     5461 gaaagcatct accttagctt gactacgatt ggcaaggaga atctcatctg acgtcctagt
     5521 ctgcaagaca gattttgcca agctagcacc catattcccc aaaccgataa atccaatctt
     5581 catctcttac tcccttatct gcccatcacc ggcaaccaca tacttgtagc tggtcaactc
     5641 tttcaagccc atgggaccac gcgcgtgcaa tttctgagta gaaatcccca tttcacaacc
     5701 aagaccaaat tgtcctccat ctgtgaaacg agttgaggca ttaacataca ccgctgcaga
     5761 gtccacttga tctgtaaagt atgctgcagc ttcagcattt tccgtcacaa tagcatccga
     5821 atgatgggtg ctgtgggatt caatgtgcgc aaccgcttct tctaaactgc tcacaacctt
     5881 aacagcaagg acatagtcta aaaactcggt gtcaaagtct tgggtctcag ctgcttgacc
     5941 tgaaacaaac tggcttgctt tgctatctag gcggaattga attggttcca gtccagcttc
     6001 cttacgctct gcaaccaaca cttgctccaa gcgaggaagg aagcttgctg ccttgttttc
     6061 atgaaccagc agaacctcca tggcattaca aacagaagga cgactggttt tagcattgtt
     6121 gatgatagac agcgccttgt cttcgtctgc atccttatcc acatagacat ggacaatccc
     6181 agtccctgtc tcgataacag gtacaatcgc attctcaacc actgcattga tcaagccagc
     6241 tcctccacga ggaatgagaa ggtctagata gcccttggcc ttcatcatag cataactact
     6301 ttcacggcta gtatcctcca ccagttgaat cacatttgga tgaatagtag tcgtctccaa
     6361 gcccttcttc aaggctgtga caatggcatg ggttgtttga taggcatcct taccactacg
     6421 aagaacaacc gcatttccac tcttaagagt caaagcagcc gcatcagacg tcacatttgg
     6481 acggctttca tagataatac cgatgacacc catagctaca cgtttttttg tgataaccaa
     6541 accattttca agctgacttg tttctaaaac ttcaccgatt ggatctggta aggcaaccac
     6601 ttcacgaatt cctcttgcca tcgcttctat acgatctgca tccaaataaa gacgatccaa
     6661 catcacatct gagattttcc ccttagccgc tgccatatcg agggcattag ccgctaaaat
     6721 ttcctcagta gcagccacta agtgatcagc catggctagc aaggcttggt ttttcacttc
     6781 ttcactagct gtgttaatcg attttttaac agcctgtacc tgttcaaatt gttcttgtct
     6841 actcaccata gtttacctct aaaattctgt aaaaagtagt tggatttcag gagtaatgga
     6901 aatccagtcg tcacggtaaa tcaagacacc cttggctttt tgagaacgca acatatcctc
     6961 caaagcagat gctccaaatt gcacgcgtcc ttttccaagt gattttccac tttccttgtc
     7021 aaatactgtc acgatatcac cgtaagaaaa gactccttct gcttcaacga taccagataa
     7081 gagaagactc tttccatatt gagagagagc ttccgcagcc cctttatcaa cccaaataga
     7141 accttgactc tgagcataga aggcaagcca ttgtttctgg gtacgaagcc ccttctcttg
     7201 agcaacaaag taagaaccat cctcggtctc ctctgccgcc tcaatcatgg aatctgattt
     7261 caaggatgag cagatataaa caggaactcc tgattccgtc gcgatagttg cagccttgat
     7321 tttggttaac atacccccag ttccgtttga cgaaccagct ccaccagcca tatcaataat
     7381 ctcacgattg atggtctcga ttctctccaa gcgtttggct cttggatctg aattaggatt
     7441 tccagtatag agaccgtcca catctgtcaa gaaaactaaa aggtctgctt ggaccatcgc
     7501 cgctacttga gcacttagag tgtcattgtc cccaaccttg agctcatcaa taacgacact
     7561 atcattctca ttgatgatag gaattgcccc acggttgagc aaaaccgaca aagcctgatg
     7621 ggcattttta taacgacgct tatccacaaa gtcatcttgg gtcagcaaga tttgtgcaga
     7681 aacgatttga cgcaagagaa gattggttgt atattcttcc aacaaaagcc cctgccctac
     7741 cgctgctgaa gcctgtttat cagcaatctt agtcggacgc tttttaaatc ctaaggctcc
     7801 aaaaccagcc gcaatggcac ctgaagacac caaaatcaac tcatgaccag cctcgtgcag
     7861 catagccaac tgctgggtaa tatcctttac cttactacgt gataaacttc catcctcatt
     7921 tgtcagagaa gaagtaccca ccttaaagac aatccgtttg tatttcatag tctcactccg
     7981 attattcata tttatccatt ataacatgaa taacaggaaa tagaaaagag ttgataggaa
     8041 aaagaaggcc ctaaaacctt cttttctact actgttctga ttccgatttg tttacactcg
     8101 tttcagagct ttgtgaagtt gaggatgtca aatcttgact agttgaaaaa tttgtaacac
     8161 tttcatgttg actcgtttct ttattttcaa gatccttttt cactacttct ttggattctt
     8221 ttatttctga atgacttgta gttttaattg tagcagattt ctcgatcgaa acatccacta
     8281 accaggcacc acttgaatcc acagtataac cttctggtgt cttaccgttc acaagtaatt
     8341 gaccattttc tttcaagaag taccatttat ccttgtcctt gatccaaccc acagctcgtg
     8401 aaccgtctgt cttgtagaaa taccaatcat ttgccttttt atgccagcct tgcttcatag
     8461 ctcctgaatc ttccagataa taatgttgac cagcaacttc tatctcacct gtcttcatga
     8521 caccagtaga atccatatag taccaggttt ctttatcttt tacccagccc attttcattt
     8581 cccctgatgg agcaaagtaa taccattgcc ccatatattg aagccatcct gtcttcatag
     8641 aaccatcaac atccaaataa taccactgct ctttatcttg aatccaacca atttccatct
     8701 ggttttcttt gttaaaataa taccagattc cagcgatttt cttccaattt ttagcagaag
     8761 caccagagtc tgtcaaatag aaccaatgat tgttccattt tttccattga ttctgtaaca
     8821 agatacccgt ttggttaaag taataccatt caccttcgat ttcattccaa ccgacagcat
     8881 actctcctgt agaatcaggc gcttgatacc accagttccc atatgcactc ttatgccaac
     8941 cagcttgaaa acttggaatc ggcttgtagg atgttgaaat attgacaaaa ccgtcttttc
     9001 gaatatcaaa aactgttgca tcatagtctt tgctggctgc gttgattctc tcaattcctc
     9061 gttctttgag ccaattaaca tactcactat caacaccatt tttccaaggt agactatccg
     9121 aagtttgaac aatcaaactc ggactcaaat ttttaatgaa atccttggta tttgatttgt
     9181 tggtatcatg gtgatgatta aacttcatca aatcaacttt tccaatgaga ggaccatact
     9241 tgtcttctgc tccatgaaca ttatctaaat cgcccccaag gtaaattttc ttgccattga
     9301 ctttcaccac gctaatcaag gaattggaat tgtcatccca aattttcttt aattcacccg
     9361 atgaatcagt ttcattttca taattataga gctgaatatc catgtcccca aactgaaaat
     9421 gagcatcccc ttgtgtgata ttttgaataa ctgaaacacc tttttctgca gcagtctgta
     9481 aaaccttatc atagccatac agattatccc atagacgttc agaattagta atacgactat
     9541 cactatattt cttaagatag actcggtcaa ctggataggt agacagtaat tcatcaacat
     9601 ttccaatatg atcactgtgg gtatgggtca ccaaaataaa atcaagtttt tggacaccca
     9661 attccttcaa acgacgaaag acacggtctg ttagaacatg cttataagac gtttcaattc
     9721 cttctctcca tggatagcga gaatcacttc catctgggaa atcataatct tctcctgtat
     9781 ccaccatggc aaaatgtcca ttgctttcaa gaataatcgc atcactgcca ccttcttgaa
     9841 cattgataaa gtggatttta tttcctgaac tttcttgagc ctgaacattc ccataccatg
     9901 acaaacctaa aaaagcgcct acaagtgcta aactagttaa tttctttttc attcttactt
     9961 tctcaatctc tccaaggttt ccttaaaaat ctctgggata tctgctttaa attccaaggt
    10021 cttacctgtt ctcggatgag taaaacctaa agtcttggca tgaagaaatt gtccatgtcc
    10081 tttcaaagtc ttgcgaggac catagacctc atcaccagcg actggatggc cgatataagc
    10141 catgtggaca cggatttgat gagtgcgccc tgtctccagt tgcaactcta ctaagctata
    10201 atcgccaaag cgttccaaga cgtgaaaacg cgtcactgca ggcttccctt tagcagttac
    10261 agcctgtttc ttacggtctt tttcactccg gccaatcggc gcttcaatta caccacgatc
    10321 attaggtaga tttccatgaa caatcgccca atatttgcgg agagactttt tatccttgag
    10381 ttcttgggca agtgctagat gcgcatcatc gtttttagca atcatgagaa gacctgacgt
    10441 atccttatca atacggtgaa caatccctgg acgcagaacc ccattgatac ccgacaagtc
    10501 cttaatatga tacatgaggg catttactag ggttccactg gtatgaccag cactcgggtg
    10561 cacaaccatt ccctgaggtt tgttaacgac agccacatcc tcatcttggt agactatttc
    10621 tagcggaaga tcctcagcca catactctaa tacctctggt tctggcacat ggtaagtgac
    10681 gacatcaccc tcttggactg tgtatttagc tttcttgact tgaccattga ccaagacctg
    10741 gcctgattta atttgttcat tcgcgagact acgtgataat tctgacaaat ctgacaaagc
    10801 cttatccaaa cgcagaccac cagtttcaat tttaatttcc atttatttcc tcttttagca
    10861 ttgcaatcaa taaaataatc actccaaccg tcagatagct atctgccaca ttgaaaattg
    10921 caaagttgat aaagtcaagg tggaacatat ccacaacaaa gccctgactg accctgtcaa
    10981 taaagtttcc aagaccaccc gcgattatta gagtcaaacc caagaccatc cagaatgagt
    11041 cctccatgtg tttatgtaaa taccaaatgg cacctatcac gacaaccaga gtaatgacag
    11101 cgaataacag ctgctgatct tgtaagatag aaaaggctgc acctcgattt tgcaggtagg
    11161 tcaagctaac gaaattgggg atccaggagc gcacttcacc cagtggaatc tgctggacga
    11221 tataggattt gaccaactga tccagcccaa tcaaaagcag tacaatgact gccactattg
    11281 ctcttttttt catgatttcc tcttttgatc aaaatattct tgcatgactt ctacgaagag
    11341 agtcccagct tgactaagct ccacttcttc acgtttaaca tagaccatgc ggttatctag
    11401 gttatccttg agacgaataa ctgtaatgcc attaacactg tcactatcta aaaatccaga
    11461 acctgtcgca taggcgtccg tccgctccaa aataccattc aaggtggcac ggtctgtcac
    11521 attaaacatc tgtgagctag cgctggtatc gacaaagttc tctgaataat aaaggtactc
    11581 gtctttctct tgagtgaaac gaaccgttgg taaatccgct aaatcctcca tgactaattc
    11641 ctctttctgg gctaaaggat gaccctcacg gagataaata tgggtatgga aaggaatcaa
    11701 ttcgatgacc tccagaccta atttttcaac ccgttgcata atcccctttt tattttgatt
    11761 gttgaggtag ataatcccaa tctcactatg cccttgcgcc acttcatcta atatttgaac
    11821 agtagttgat tcaaaaatac ggaagttctt atagtcagga tagcgctctg aaaaggccgt
    11881 aatagttggt ggcaagaagt catagtgctg gctagcaaca gaaaattcat ctttttcttc
    11941 ttcaggattg gcatactgat tttgaaaaat atcaaatcct ttaaccaatt cttgcgattt
    12001 ttcataaaat tccatcccac gacgggtcaa gaaagtccct gagctggtcc gacggaaaat
    12061 cttaaagccc aactcttttt ccaaatcacg aacagaaata gacagactcg gctgactaac
    12121 atacatcttt tcagcagctt cacgaaaagt accactattg gcaatagcca caacatagcg
    12181 taattgttga atgttcatct tctaccccca acttctttat cttttcatta taccatattt
    12241 tagaagtttt ccaaaaagga aaaaagaaca tcctattctt cttaactatc ttcactatct
    12301 gcctttctca agtcaacctt attttcaaaa tcaattcaaa aaaataactg gatacacacc
    12361 actacaatat cgaaaagaaa aacgttgact atttgtgaaa aaagaaaatg ccggaaagtt
    12421 ccgacattat tttagtaagc taacttcctg aaaataggag caaccacaca tcccagaagg
    12481 cagtgatatt aatgagaata tgaactagta ttggatagta gagattttta gtcatgcgat
    12541 acaatagagc taaaataaga cctccactag cataaatgaa aaaagcaaga ggagttaaag
    12601 caaaattgat atgaatataa ccgaaaataa tagcagaaag caaaacatct ccgtaccaag
    12661 gtgagttttt gaaaaaggtt gtcataagca cacctcgata aatcaattcc tcagcaatag
    12721 gggcgatgaa gcaaacgatg agcaagaaat aagggaactc ctgtctcccc atcatttcta
    12781 ccgtttcatt caaagaaatc tgatttgaag acagggatat gaaatacgaa aagaggaagt
    12841 cagacatata tgaaatgatg tagcccagta aaaggtagat gaagtacctc agctgccact
    12901 tgaaatgaaa aattttcttt cctgcaaaaa ccagtagata gatgaccgct aagaaaagaa
    12961 ctccgctctc catcaaaagc agaatctgaa aaaattcacg acttgctggt agatagggct
    13021 ttgcgagata attgaaaagc ctccaaaacc aagttgattt gtaaaaaatt aaggacaatg
    13081 ctagtaaaat ttgaatagct cgttttttca tattaaatcc tctgttttct ccccttgttt
    13141 cttcatattc ctaataaatt ttacttaaaa tctcgcagca cttcctttgc aaagatgatt
    13201 acctcctcca atatatcctg aatcataggt gcctctggga caaaatccat gaaccagcag
    13261 agttaatact ggttctattt cccaatcgta cctaccatta ggatttttac catatggatc
    13321 tgctccacct tgtatcagct ctaattcttc atttgttaga tttatatttt ttgtcattaa
    13381 aatcatgata tatttcctcc attattcttc agattagttg agtaatctgc tactcatacc
    13441 tacttacaaa aaaactatta tattaagttg gttttttaat ttgtctagtt gcaacattga
    13501 caaacatagt atagcatatc tttgcgaaat atcctcttca aatcatgaat tgtcatcaaa
    13561 acatcttaaa ctataaaatc aattagtctc aagctttcta tcaatttctt ctccaaaata
    13621 tgctataata atagcaaaag ataaagaagg aagacctatg attaaactac tagccttgga
    13681 tatggacggc accctcctca atgaagccaa ggaaatccca caagctcaca ttactgctat
    13741 tcacaaagcc attgaaaaag gtgtcaaact ggttctctgt acgggtcgcc cccttttcgg
    13801 tgtcctcccc tactacaaaa aactggagct tgacctccag aacgaatata ttattgttaa
    13861 caacggttgt tcaactcacc agaccagtga ttggagtcta gttgactgga aagaactgag
    13921 tccagctgac atcgaatacc tctatgacct tgctgaaaag agtgatgttc agttgacact
    13981 ttttgacgag tcacattatt ttgttctcgg tgacaagccc aatcaagtta ttgaaaatga
    14041 tgctaaacta gtattttcag acctgactga aatttctctt gaagaagcga ctagtggaaa
    14101 gttccggatg ttccaaggta tgtttttagg aacaaaagaa caaacagacg attttgagca
    14161 gcgttttgct gaagagcttt gccaacgatt cagtggagtt cgttcgcagc ctgtcattta
    14221 tgaagcaatg ccgcttggaa cgacaaaggc tactgctctt tcacgactag ctgagatttt
    14281 gaagattgat tcctcagaga ttatggccat gggcgatgct aataacgata tcgaaatgct
    14341 ccagtttgca gggcttggga ttgcaatggg aaatgccagc gattatatca aatctcttgc
    14401 ggatgccgtt acctcaagca acgaagaaga cggcgttgcg cgtgctattg agaaatatat
    14461 tctataaaaa agaaaagcaa atagacagaa gttactgcta tttgcttttt tgctaatatt
    14521 ttaaaatacg gactaatcaa tagacaagaa tagcgaatca taaaaatcga tttactagat
    14581 gccatagtat cttatagtta ctaataagaa gttagtctag cagaatgaat ctctcatttc
    14641 tcccacacta atctgtaatt tgtctataca tttctggtct tctatctcga aacaagcccc
    14701 agtttaggcg ttcacttgct cccttgtcta ggtcataagt agctaacaga acagcttctt
    14761 cttgtctttc agctcgttct agaatagctc ctgtttcatc cgtcataaag gaggaaccgt
    14821 agaagtcaag actggagctc tgtccgccat tttcctcact aggagtaacc tcctctaaac
    14881 cataacgatt ggctgcgatg actggaacaa tattcgctgc tgcgtgccct tgcatagtac
    14941 gttgccagtg accacaacta tctgtatcca aaattggctc tgaaccgata gctgtaggat
    15001 aaaagagcaa ttcagcacca ttcaatgcaa gacagcgcgc tgtttcaggg aaccattgat
    15061 cccaacagat accgatacca atcttagcat agcgagtatt ccagaccttg aaaccagtgt
    15121 taccaggcgt gaaatagaat ttttcttgat aataatggtc atctggtata tgggtctttc
    15181 gataaacgcc cagcacttcc ccatctgcat caatgacggc aatagagtta tacaagacat
    15241 taccatcttt ttcatagaaa ctgattggta aaacaacttg tagttcctta gcaatcacct
    15301 taaaatgctg aatggcagta ttttccgcta cagattgggc atactggtag tagtcatact
    15361 gacgttcctg acagaaatag ggatgttcaa acaactcggg caagagaata atttgggctc
    15421 cttgctcagc agcctgacgt actaaacgct ctgcggtttg gatatttgtt gccacatcct
    15481 tagcgcattg catctgaatg gttgcaactc ttacatttct catctttttc tcctattctg
    15541 gaatttgttg ggtgatacag tggatattgc caccacctaa gagaatatct ctggctggta
    15601 ttccgacaac tttacggtct gggaaacact tgctgaggat atctaaggcc acttggtcgt
    15661 ttacatcctc aaactgtgga accaagacag ccttgttggc gatataaaag tttacgtagg
    15721 aagctgctag tcgttcacct gcgtatcgct tttcttctcc ttcttcatag atgtagcctg
    15781 gcaaatcttc ttctgtcaca acttgtcgaa ctgcagggat aggcaattta tgaatggtga
    15841 agtgacaacc ttttgcatct gtttcctgtt ctaagagttc gagatctgct tttgacatgg
    15901 catactgggg atcattttcg tcatctgtcc aagccaaaac aagctcagca ggaccaacaa
    15961 aggcagcaac attatcgacg tgttcattgg tttcatcctg ataaatacca taaggaagcc
    16021 aaataacttt ttcagcacca agactttcta ataatgtgtt ttcaatctcc tctttagtca
    16081 agttaggatt gcgaccagga ctaagcaagc aactttcagt tacgagaata gttccttgac
    16141 catcgctatg gattgcgcct ccttccagta caaaaggttt agcatcatag acaggccttt
    16201 ccaaggcctc agcaaaacga ctggctactt ggtcatcctc ttcataatct tgataaagac
    16261 catcataggt gcctccccaa gcattgaagg cccaatccac ggctaatttc ttacctttat
    16321 cattgacgag aatggttggg ccagtatcac gcgcccaggc atcattggtg ggaatgtcta
    16381 aataaacaac cttgtctcca agataggatt gggcttcaga tagataggcc tgctccacca
    16441 aaagatagac tctttcccct tctgctatgg tctcgataat ctgagtaaat gctcttttag
    16501 cagcctttcc ttgaaaaggc catgatcctg gtcgagtcgg ccatatcatg agggtaccat
    16561 gatggggttc gtactctgct ggcatgtgat agcctaattt ttttggactg tccatcatga
    16621 taatctccct ttaaagtctt gatagccaaa agctttgagt aagctacagt ctccctgttc
    16681 gtccatgaga tagagacttg gcaatccaat accattaaag gtattatttt tgacaaaaga
    16741 ataaatggcc atgtcttgaa aataaagtct gtctccgatt tggactggat tttcaaaact
    16801 ataatcacca atcacatcgc ccgtcagaca ggtattagaa gaaagtctgt aggtatgggc
    16861 tttttcctgt gactcaaagc catttctcaa aggtggacga tagggcatct caagtacatc
    16921 aggcatatgg caggtcgcag aggcgtctaa aaccaagatt tccataccgt tttctacaat
    16981 atctaatacc tcagttgcta aataacccgc attaagcgca atggcttcac caggctcgat
    17041 atagatttca agattgtaag tttttcggat acgcttgatt tctgaaatca gcaaatccac
    17101 atcgtaacct tctcttgtaa tatgatgacc accacccata ttgagccatt ttacctcatg
    17161 taagtaggga ccaaactgtt cttctactgc tttcaaagtt gtttgtaaat catctgctcc
    17221 ctgctcgcaa agggtatgaa aatgaagtcc gtcaaccaaa tctagcaaat cactcggaat
    17281 cttgtctata gtaactccaa agcgagaacc tggtgcacaa gggtcataga gcgcgtgatc
    17341 tccttgagtt gaacactgag ggttgaggcg caaaccaaca ctgacaccag cctctcgaca
    17401 acgcggaccg tgtttacgca actgtctctc tgagttaaag actatatggt ccattatctc
    17461 tagcaattcc tccaagtctg catccttgaa agcaggcgca aatacatgga cttcaccagg
    17521 aaattcttcc cttgccaatt tggcctcata gagtccacta gctgtcgtac ctgatagata
    17581 ctggctaatc aagggataag ttttgtagag ggaatatgcc ttctgggcaa gcaagacctt
    17641 gcaaccggcc tcttcttgta catattgtag aatgcggcaa ttagcttcta acttggccaa
    17701 gtcaataaca taggctggtg ttggtacttg ttctaacttc attagtccac catttgtgga
    17761 ttttcaacca caacccatgg caaaccatac tcattcaaag cttccatgaa tggatctgga
    17821 tctaactcct caaggttata cactccagct tgtttccaag ttccgttcat gactaatttt
    17881 gtcccaatca tggctggaac tcctgtcgta taagaaatag cttgcgaacc aacctctgcg
    17941 taacattcct gatggtcgca gacattgtag atatagatag tctttttgac accgtctttg
    18001 acacctgtaa agatacatcc aatattggtt tttccgactg tacgtggccc aagactggca
    18061 ggatctggaa gcaaggcttt caaaaattga attggaacaa tttcttggcc gttaaagtta
    18121 atggtatccg tacgaaggag tccaacattt tcaagacatt tcatgtgcgt caagtaagat
    18181 tgaccaaaag tcataaagaa acgaatgcgt ttgacacctg gaatgttctt ggccaatgat
    18241 tcgatttctt catggtgaag gagatacata tctttttgtc caacttgagg gaaatcatac
    18301 tcacgcttga tagacatagc ttcgacttcg acccatttcc catcttccca gtaagaacct
    18361 ggcgcagaaa cctcacggag attaatttct ggattaaagt tggttgcaaa tggataaccg
    18421 tggtcaccgc cattacagtc taaaatgtcg atataatgga tttcatcaaa atagtgtttg
    18481 agggcataag ctgaaaagac actagttaca cctgggtcaa aaccagaacc aagaagagca
    18541 gtcaagcctg cttctttgaa tttctcttga taagcccact gccatgagta gtcaaagtag
    18601 gctgtaaaac caagttcctt acaacgtttt tcgtagatag cacgccactc agggtcttct
    18661 gtgtcttctg cttcgtagtt ggctgtatcg atatagtgaa cacctgttgc caaacaagca
    18721 tccataatgg ttaaatcttg ataaggcaga gctacattca aaacagcttc tggtttgtag
    18781 ctttcaatca gggcaatcac ttcttcaacc ttgtcagcat caagtgctgc agtttcaatt
    18841 ttagtacttg ttttgccttc tagcttcgct ttcaagtcat cgcattttga cttggtacgg
    18901 ctagcaatca taatctctgt aaatgtttcg ctatcttgac aaatctttga aatagcaact
    18961 tgggcaacgc ccccacaacc aataactagt aaacgactca tttttttcct tcctcttctt
    19021 ctaaaatgtc ctcaacatac ttgggcaaca taaaggctcc cacgtgtaag tttgcagtgt
    19081 agtattctgt gaaaagctgg cgttttttcc agccttcctt gtcaaaatct ttgacagggt
    19141 ggtatttttt cgatgcaaat ccaaacaacc aatagccagc tgggctagtt ggaatatggg
    19201 cctgataaac ccgactgatt ggaaaggctt gattgacctt gcggtgcatg cttcggcagg
    19261 ccgactcatc ctcgtcaaag aagggactcc catgctggta aatcatgatg ccgtcttcct
    19321 tcagagctcg ataactattg ccgtagaatt ccttggtaaa gagtccttcc gtatggccaa
    19381 atggatctgt cgcatcgttg atgataatat cgtaatcatc ttcgcagttt cgcaaaaagc
    19441 gtagcccatt ttggtagtaa atggtaacac gaggatcatc tagccctgca gcaaagtctg
    19501 ggaaatactc acgacagacc tcgaccaaca tctcatccgg ttccacaata tcaatttgct
    19561 ccagttcagg atagagggtt aatacttggg caacaccgcc gtcaccaccc ccaataacca
    19621 atactttctt tggatttggg tggacagcca tgggaacgtg aacggtcatt tcattgtaga
    19681 cgaaatcatc cgcatctgag aacaagacat ggccatttaa aatcagtatt ttcccaaaag
    19741 ctggcgtatc caagacttcg atatcctgcc attcactttt tccagcgtaa agttgcttgg
    19801 ctgttctcag agacaatttg acatctggag tatgaacttc agaaaaccat aaatccatct
    19861 atttctcctt atattatatt tagcgcagtt agctactgcc aaagcccaag tggtatactt
    19921 ggaataagcc actgtggatt agttcatttc ctccaatcgc ttgacgattt ccgaaaggct
    19981 ctaaccacag ttttttgttt caattggtaa tgagtttgat aacgcataga cgttttatct
    20041 cgtctaaggt aacaaggcaa aaagttcgtg gataacccag tagacttgaa atactatatc
    20101 tggagatact cccatgcttt atgtgggcat tgatgtcgct aaaaataaac acgatgttac
    20161 agccttgaat gttccaggaa aaactgttct taaaccactc actttttcaa ataataaagc
    20221 tggttttgaa ctcttagatc tgtctcttcg acagctcaac caagactgtc tcatcgctct
    20281 tgaaaatacc ggacattatg cctttaatct actgaacttc ctgcatgaac aaggatataa
    20341 agtttacacc tacaatcctt tgctcatcaa gaaatttgcc aaatcattat cacttcgcaa
    20401 aactaaaacg gataagaaag acgctcatgg catcgctctt aaacttcttt ctgaccccaa
    20461 tcgtgaacaa tttcaacacg ataatcggca agtagaacta aaaatactgg ctagacatat
    20521 tcatcgtctc aagaaaaaac agtctgattg gaaagtacaa tacactcgtt gtcttgatat
    20581 catctttcct gagttggata aaatcgttgg aaagcattca gaatatacct accaactctt
    20641 gacacgctac cctaatcctc agaaaaggct tgaggcagga tttgataagc tgatagaaat
    20701 taagcgattg accgcttcta attcaagggc tttttgccgt gctctaaaat catcgccaaa
    20761 tcatcaaatt gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc
    20821 tttctcttaa taacgttgat atgattgacc tctggatctt ccgtcccttg gagggagcaa
    20881 ccacgttcct tggcgaattg gatatagtcg acaatttctc gtgtaatgcg ttcaccagga
    20941 gccaagatag gaatacctgg agggtaacac ataacaaatt ctccacagac ctgtccaaca
    21001 gaatcatcca aagttaaact ttttctttct gaatagaagg cttcttgcgg agacagcact
    21061 aactcgggct gaatatattc tcctgctatc aaatcttttc catctcttga atagagtctc
    21121 ttaatatcag ccagagcacc aaccaagcgc tcgatgtctt ggatgcggtc gccgatggaa
    21181 atataggcca agatattgcc gatatcacca aactcgatct gaatgtcgta ttcgtctcgc
    21241 aagaggtcat aaacctcgat acctgttaag ccaata
//
LOCUS       DBDESG010000042        21146 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.042, whole genome shotgun sequence.
ACCESSION   DBDESG010000042 DBDESG010000000
VERSION     DBDESG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.042"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            832..993
                     /locus_tag="ACKB38_001471"
     CDS             832..993
                     /locus_tag="ACKB38_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980224.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1085..2764
                     /locus_tag="ACKB38_001472"
     CDS             1085..2764
                     /locus_tag="ACKB38_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGL7980225.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLHIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENISIQTMEGRIQKAGEGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGNKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKLDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3032..3598
                     /locus_tag="ACKB38_001473"
     CDS             3032..3598
                     /locus_tag="ACKB38_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980226.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3920..4108
                     /locus_tag="ACKB38_001474"
     CDS             3920..4108
                     /locus_tag="ACKB38_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGL7980227.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4170..4928
                     /locus_tag="ACKB38_001475"
     CDS             4170..4928
                     /locus_tag="ACKB38_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGL7980228.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            4986..5840
                     /locus_tag="ACKB38_001476"
     CDS             4986..5840
                     /locus_tag="ACKB38_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGL7980229.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5952..6836
                     /locus_tag="ACKB38_001477"
     CDS             5952..6836
                     /locus_tag="ACKB38_001477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGL7980230.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6999..8738
                     /locus_tag="ACKB38_001478"
                     /pseudo
     CDS             6999..8738
                     /locus_tag="ACKB38_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            9013..9468
                     /locus_tag="ACKB38_001479"
                     /pseudo
     CDS             9013..9468
                     /locus_tag="ACKB38_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9913..10389
                     /gene="pezA"
                     /locus_tag="ACKB38_001480"
     CDS             9913..10389
                     /gene="pezA"
                     /locus_tag="ACKB38_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HGL7980231.1"
                     /translation="MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            10389..11150
                     /gene="pezT"
                     /locus_tag="ACKB38_001481"
     CDS             10389..11150
                     /gene="pezT"
                     /locus_tag="ACKB38_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HGL7980232.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            11178..11954
                     /locus_tag="ACKB38_001482"
     CDS             11178..11954
                     /locus_tag="ACKB38_001482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980233.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHPHILHGVEISAADKLHIVCI
                     YDYEQESWVNQWLSENIISEKDGSYQHSLTIMKDFNNQKIVNYIAHFNSYDILKKGSH
                     LSGAYKRKIFSKENTRFWSLILTRKNLRNNLIFSIKKLVY"
     gene            11957..12334
                     /locus_tag="ACKB38_001483"
     CDS             11957..12334
                     /locus_tag="ACKB38_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980234.1"
                     /translation="MGQKVVAMLDFLLAYSDYSKDFRPLIIDQPEDNLDNRYIYRHLV
                     QQFRDVKAQRQIILATHNATIVTNSMTDQVVIMESDGVNGWIESQGYVSEKYIKNHII
                     NQLEGGKDSFKHKMSIYETALSE"
     gene            13166..13522
                     /locus_tag="ACKB38_001484"
     CDS             13166..13522
                     /locus_tag="ACKB38_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HGL7980235.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            13527..13892
                     /locus_tag="ACKB38_001485"
     CDS             13527..13892
                     /locus_tag="ACKB38_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGL7980236.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            13879..15702
                     /locus_tag="ACKB38_001486"
     CDS             13879..15702
                     /locus_tag="ACKB38_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
                     /protein_id="HGL7980237.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            15816..16682
                     /locus_tag="ACKB38_001487"
     CDS             15816..16682
                     /locus_tag="ACKB38_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980238.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
     gene            16835..16969
                     /locus_tag="ACKB38_001488"
     CDS             16835..16969
                     /locus_tag="ACKB38_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980239.1"
                     /translation="MRSLFRKIVALLVIGLILLGTAGGTQVHKMARGIDPGPANGIYR
                     "
     gene            17273..17524
                     /locus_tag="ACKB38_001489"
     CDS             17273..17524
                     /locus_tag="ACKB38_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980240.1"
                     /translation="MSSQMKAFLNELQGNMEVVGEFFDNPEKVIRKFGIVGREKEALL
                     IRDLNDMDDYALSIQNSVASPSGAHSSTCHFVRQDLQSA"
     gene            17753..18055
                     /locus_tag="ACKB38_001490"
     CDS             17753..18055
                     /locus_tag="ACKB38_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980241.1"
                     /translation="MKNKYDVKRIIPDELSESLDIFLKNYSETGLSDYNTYLFYGFIL
                     KSYKLPRENRYSIKLLVKELQNRGLKVTLIINIYYHALNCLALNDGLKIYGEDFLI"
     gene            18052..19398
                     /locus_tag="ACKB38_001491"
     CDS             18052..19398
                     /locus_tag="ACKB38_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGL7980242.1"
                     /translation="MNYEKYLNYLDYETEIEDAYHNLLLEYKISDNFSDEHWLYNLPS
                     NITQSKGFKIHLSASILNANLVAKKFFDFIFSREKKINFKILVSIKELSLQNTGLNGY
                     SQVGKFITIYPKDNKEFQRLLHKLEILYKGVKGVNIPSDFRFQLSEVVYYRYGEFVKD
                     STFKDKRDKKIPSNVNVPIRDYYIPRYNTIPDQYIILEVISKNAKGGVYKVFNTQKRV
                     YSLLKEASDLSLVDFTNRDSVNRLINEREILVELEKEEFTPKVFNDFYIKNSYFVEFE
                     FIIADKLSEYNMVSGSYDWFLTLIDYMEIINSKYNLSYRDLSFNNILIDNRNNIYIID
                     FEHALRKETQIEEKKLPLFGTPGFYETNLNLLNNQPEDIMGLVLLLYWSQNLDDFNVF
                     SKMSFLEAMEYAMNFNSRRLDTLDKSDWLYDIYKKAFNYKYDTFSELKKDFIEVLK"
     gene            19430..20329
                     /locus_tag="ACKB38_001492"
     CDS             19430..20329
                     /locus_tag="ACKB38_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGL7980243.1"
                     /translation="MNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKSTLINSI
                     VGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLNLAGKFG
                     KNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDEPTVGLD
                     TESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDMRDFVDN
                     STIKLNFSMQNRISRYQIEFLENFRFKVHIEDNDSFTIEVPIEEKILDVINEVGKACE
                     IKNFSTSKLTLQESYLQRIGGEK"
     gene            20326..21126
                     /locus_tag="ACKB38_001493"
     CDS             20326..21126
                     /locus_tag="ACKB38_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980244.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGLIISQFLQPIL
                     YVVFIIIGLNSSIKNIQFNDIKTSYAEYTIIGVIALLIIGQMTQVIYRVTIDKKYGLL
                     ALKLCSGVRPLYYILGMSIYSILGLIVQEIIIYIITLAFEINIAMDRFFYTVLLSIVV
                     LLFWDSLAILLTMFINDYRRRDIVIRFVLTPLGFTAPVFYLIDSAPSIVRWIGQLNPL
                     TYQLTILRNFYFKNSTTLELVFLLLTSLLVLISVSFIIPKIKLILIER"
BASE COUNT         7590 a         2841 c         4194 g         6521 t
ORIGIN      
        1 taaactatcc agatacatgg aaagcagtgg taagacctat caaaaccact atgtgacaat
       61 cttaaaatgg tatgaagaag ataaagacaa actaagacag aaaggtttaa ataaaaaaat
      121 gaattatgat gtaggagaat ctttatagat aaaaagaggt ggaaaggcac tattaaagac
      181 tttaagtcta aaaaggtatc aaagtatgcc agagataaaa aaatataaat atgacacttt
      241 aaaggcgatt agaaaattaa agaaatgcta gaagactttt agatgacagt taccaagagg
      301 gtctaatttt cactgctatt tatgcagtgg gaattggacc tttttatata agcaaattct
      361 atttcaaaat tggttaccaa gaaagacttc ttttcactgt taagtataag agggtaattt
      421 atagaatttt gttacatcaa gataaatatt ttctgatggt caaattttaa aaagattaat
      481 aactctcgat aaattttgaa ccttaacaat tgaatagacc aagacaagac gttaattatt
      541 tttggagcgt aataacctat ccgtcaagat ctttcggctg aataattcgg caaaaaaagt
      601 tctgctaagc aaaaagaaag tgagcgataa gtaagagttt gaatataggg agggataccc
      661 tatccgctat gaagaaaatg agggtaatga tacttctaag gttgaagccg gctcatccgg
      721 aatagaggcg gaagtgagat tcctatgttg ctaatccaaa gcacttgtca atgtcaaaaa
      781 acttaattaa acaaataatt attagatata aaactttaga ggaggtatat attggacaat
      841 gactggaatg gattggctga tttaatagca aatctgattg caaaatatgc tggtgcttta
      901 gatttagata atctacccga tccaacgcca gcaaaaaatc aagagatgaa aaatagtttt
      961 gacatggcaa aaacacaaat tgagacggac taataaaagt gttataatat acttgaaatg
     1021 atagtccaaa cttaaattaa aaaaattata tatgatatag aaaaattata ttgaggttaa
     1081 tactatgtca aaagaaaaaa taaaagtata cctctataca cgagtatcta cgtcaataca
     1141 gatagagggg tattcgttag aggcacaaaa atcacgaatg aaagcttttg ctatttacaa
     1201 cgattatgaa attgttggag agtatgaaga tgccgggaag tctgacaagt ctattgaagg
     1261 tagaatacag ttcaatcgaa tgatggaaga tataaaatct ggaaaagatg gagtatcttt
     1321 tgttcttgtg tttaagttat caagatttgc aagaaatgcc gctgatgttc tttctacact
     1381 tcatataatg caggattatg gagttaatct tatctgcgtt gaagatggca ttgattcatc
     1441 taaagatgct gggaaactaa tgatttctgt tttatcagct gtggctgaaa tagaaagaga
     1501 aaacatcagt attcaaacaa tggaaggtcg cattcaaaaa gcaggggaag gaaaatggaa
     1561 tggtggcttt gctccgtatg gatataaact tgaagacggc aagctgttta taaatgagga
     1621 agaggcagtt gcaataagaa cgattttcga ccagtatgta aatactacga taggagccaa
     1681 tgggatatct aaatacttag agaatcatgg aattagaaaa atcccaagac agaatggtaa
     1741 gaatcctttg tttgatgcag gtcttataag aaagatatta aagaatcctg tatataatgg
     1801 gaaaatagca tttggaagaa gaactttaga aaaagttcat ggtacaagaa atgaatataa
     1861 gcaggttgaa caagatgaat atctaatatc tgaagggata catgaagcta tagtttccga
     1921 tgaagtttgg caagctgctc aggttaagct aaaatctcaa gcaaagaaat atgagcatgt
     1981 gaataaagga aaagatacac gcacacactt gctttctgga attgtaaaat gcccgatatg
     2041 tggagtggga atgtttggga acaagtgtat caagaaaaag aaagatggca caaagtataa
     2101 agatttttat tactatggtt gtaaccatag gcagatgata agaggtaata agtgtacatt
     2161 cagtaagcaa attagagaag aattgttaga tgatgctgtt gcagaggtga ttgtcaagat
     2221 agtaagtaat ccgaaatttg cttctatgat gcaagaaaaa atcaacatga aggtggatac
     2281 ctctgaaata gaaaaagaaa tagataatta ccaaaaggaa ttgagaaaga gtcattctac
     2341 aaagtttaag ctaattgagg aaatagataa tttagatgtt gaagataagc attataagag
     2401 aaggaaacag gatttagatg atagactata tcgtatgtat gacaaactag atgaattaga
     2461 atcatcacta attgatgcga aagcaaagaa acagactatt gaagctgaaa agcttacagg
     2521 agataacata tataaggttc tgatctattt tgataagctc tataaagtca tgaatgatgt
     2581 agagcgtagg cagttaattt cagctttgat ttctgaaatt caagtttatg aagaaaaaca
     2641 atctaacgga caatggctaa aatccattac ttttaagcta ccaattatag aagaaaatct
     2701 caacataggt ttggacaatg atgaacaagt tgaatgtgta agtttattag agaaacgtag
     2761 ttaatcctca aaaggttccc cgaacctttt gagtcccact gacgcatcac gctgaggcgg
     2821 tatcactgct tgtacgagtt gaagcatcag caaagtaaaa gtagatgttc agccaatgcg
     2881 acgaggtgag accgaaggtc gataaggtgt gttctgggta taagactcgt aaagtaagta
     2941 ggaaatatga tgagatgata cagagagcct gaattggtca ggtgacgtgc ttatttacat
     3001 tagcctgata atgaaaatgg agagattatc gatggttata ttggaattgt accaaaatga
     3061 ttattctaaa gatttagtag catttgattc catagaagat gggaaagcct ttgtagctca
     3121 aatcccggga tacactctgg aaacagaaga tggttttgaa gtggagtatt ttaatcctaa
     3181 aaacataccg gattatatgg aaattatttt taacggaaat attgttcctt tatctaaatt
     3241 tatgttcgat cctgaggaaa atgtaaacat tatttggaaa gagatttcaa atttatccct
     3301 caaaaatgat agagtgatcg aaggatattc aaaaattgat gcatatgtcg ttaacaacca
     3361 tgaagttaaa gcctatgtcg aaacaagaga aacgaactat cgtaaagcaa aagacttctt
     3421 agaaagccgc ggatatgaaa tcgacagaag ctttttcgga agtgaagacg gtgaagcgat
     3481 tctttacaga aaaaaaggga tcgaagtttg gcatttcttg tgtcacttag acccaatgtt
     3541 tgtagagatt gaagatgtag aaggatatgt taaagaagaa gttggagaga ttcaatagtc
     3601 aaccaaagtt ccttgtgagt atataccttt gctagtaaaa gagtcaaaac gttataacga
     3661 agggggggaa gaaaaccctt gagtgtaagt ctttttcttg tttagaggga aagtattaga
     3721 gtagccgctc ataatttata ctagatttga taactgattt gagacagatt ttatttctat
     3781 aaatattgtg aaaatttttt aataccaaag atttaaagtt actgtgttaa aaatcctctc
     3841 cacttttaaa ctaatgtatt ataaaagcca gttgacacta gtgttacacc tgtgatagaa
     3901 ttaacttgag gtgatgaaaa tggcaaccga taaaaataga ataatgatta gcttggatga
     3961 taaaaatcta gagaaacttg aaaatttagt agaagatgct agagatagaa ggggaatgcg
     4021 actaacaaag tcccaggtta ttgaattact cctaaatacc gttgattatt tcgatgatat
     4081 tatgggggct atttactcaa aaaaataaga gcaaaattgc tattattttt tacaaattgg
     4141 tgtaacacta gtgatatagg agtgatgaaa tggttgttta tattagacaa agtaaattac
     4201 caagtgaggt ttcaattaat aaatacaatg ctcaagtagg tgcctactta caaggagaag
     4261 aagttatttt atatcaatct ttttctgaaa taaaagagtt aacaagtgaa gatatagttg
     4321 tagattatat aatggagact agagcattac taaaaatgat gggcttaaac gttccggttc
     4381 atgactatcc tattgagctt aaagagtttt atggtcgaaa gatttatgct ggtattttag
     4441 gagagattgt gaatatacct gataattggg gaaaatttat taagcctaaa gctggttcaa
     4501 aagtctttac tggaagagtt gttaatggaa cccatgattt aataggtatt ggtctacctt
     4561 tcgactatcc tatatggatt agtgaggttg tagaattcat agctgaatgg cgttgttttg
     4621 tgttagatgg tcgcgtatta gatgttcgac cctatacagg tgattatcat gcacaatttg
     4681 atgcaagtgt aattgatgaa gcgatatcat gttggaaaga tgcgccaata gcttatggac
     4741 tagatatcgg tgttactcgc gatggcagaa cacttgttgt tgaagtaaat gatggttatg
     4801 cattgggaaa ttatggccta tctcctttaa aatcaatcaa ttttcataga gctaggtgga
     4861 aagaaatggt aaaaccctat tttgaaaaaa atgaaatttt taaaatccaa caggatgtta
     4921 ttttctaaaa taaatatggt agataaccaa tataaaaagc atcaaaatga acaaggagga
     4981 atctgatgct tggagcaatt attggagata ttgtcggttc tgtttacgaa tggaacaata
     5041 tcaaaacaaa ggactttcct ttatttcgga aggactgctt ttttacagat gacacggtta
     5101 tgacctgtgc tgttgcagaa gcaattatga atggtggaca aaaagatgac ttcattgacg
     5161 cgatgaagaa atatggcaga atgtatccga atgctgatta cggtgctcgg tttaatgcat
     5221 ggctaaacag cgataaccgt gagccttata atagctttgg gaatggatca gctatgcgta
     5281 tttctccatg tgcttggatc atggactgtg gtttttatgc gaaaactggt atatggccat
     5341 catctagagg acttacgagt ctttctgcag aggtaactca taatcatcca gaaggtgtca
     5401 aaggcgctat ggctacagct gatgctatct ttctatgtcg tttttacttt ggtggttatt
     5461 gtagagagta cgagcaatca attaacgaca atcctacaga gtgtaaaaga cgaattaagg
     5521 attatataga aaaagaatac gattacaatc tatctcaaac tttagatgaa atccgtccta
     5581 actatcgttt taacgaaaca tgtcaggaaa ctgtccctca agccattatc gcctttcttg
     5641 aaagtagaga cttcgaagat gcgataagaa atgccatctc acttggtggc gacagtgata
     5701 ctctcgcagc aatcacttgt agcatagcag aggcagctta cggtattcct gattggatca
     5761 aggataaggc ctattcttac ttggatgaac ccttaaagga tgtagttagg cgatgggaaa
     5821 atagaataaa agcgtattaa tctccagtac gtcagatttc tcttttctgt attttcattg
     5881 tataatagga ttacatttac ttgcatacga cgaaatgtaa acatttctgt ttaaaattaa
     5941 ggataaaatc aatgaaaaaa gcaatggtaa ttatcaaccc tacttctggt ggcgagaagg
     6001 ctttggatta caaagaaaag ctggagaata aagcaaaaga atactttgaa tatgttgaaa
     6061 ccaaaattac cgaaaaagcg ctggatgcaa cacattttgc tgaagaagct tctcgtgagc
     6121 agtatgatgc agtggttgtg tttggtggag atggaactgt caatgaagtc atttcaggta
     6181 ttgatgagag agactacatt cctaagttag ggattatccc aggcggtacg ggtaacctca
     6241 ttacaaaact tttggaaatc aatcaagaca tcgatggcgc aattgacgaa ctggattttg
     6301 atttaaccaa taagattgat atcggtaaag caaatgacaa ctattttggt tatatcttta
     6361 gtatcggttc tctgcctgag gcgattcaca atgttgaaat agaggacaaa acaaaattcg
     6421 gtattttaac ctatgctgta aataccatga agtctgtcat gacagatcag gtctttaaca
     6481 ttaaggttga gacagaaaat ggaaattatg ttggtgaagc tagccatgtt ttggtccttt
     6541 tgacaaatta cttcgctgat aagaaaatct ttgaagaaaa caaggacggc tatgccaaca
     6601 ttttgattct gaaagatgcc tctatattct ccaaattatc cgtcattcct gatttattaa
     6661 aaggggatgt tgtcgcaaat gataatatcg agtatatcaa agcgcgtaat attaaaatct
     6721 cttcagatag tgaattggag tcagatgttg acggagataa atcagataac ctacctgtag
     6781 aaatcaaagt cctagctcag cgagtagaag tattttcaaa accgaaagag gattagtata
     6841 tagagaaagc cttttttaag gctttttgta tactttaaaa gatagttcct ttaacaacgg
     6901 acattccttg caaatagttt acaaaaatag tatactggat tcattgagtt tgaaaacgtt
     6961 tgcgtaaaat ttgaatgaat actttaggag acaaattgat ggaattgagt gctatttacc
     7021 ataggcctga gtcggagtat gactatcttt ataaggataa gaaactccat attcgaattc
     7081 gaactaagaa aggggacatt gaaagcatca acttgcacta tggggaccct tttatcttta
     7141 tggaggagtt ttatcaggat acaaaagaaa tggtcaagat aacttctggt accttatttg
     7201 accattggca ggttgaagtg tcagttgact ttgcacgtat ccagtatctc tttgagctca
     7261 gagatacaga aggtcaaaat attttgtatg gcgataaagg gtgtgtggaa aattctctag
     7321 aaaatcttca tgcaattggg aatggattta agttgcctta gcttcatgag attgatgcct
     7381 gcaaggttcc tgactgggtt tcaaatacgg tatggtatca gatatttcct gaaagatttg
     7441 ccaatggcaa tgctctatta aacccagaag ggactttaga ctgggattca tctgtcacac
     7501 ctaagagcga tgatttcttt ggtggtgatt tacaggggat tattgatcat atgaattact
     7561 tgcaagactt gggtattact ggactatatc tttgtcccat ctttgaatct acaagcaatc
     7621 acaagtacaa tacgacagat tactttgaaa ttgaccgtca ttttggagac aaggagacct
     7681 ttcgggaact ggtggatcaa gcgcatcatc gtggcatgaa agtcatgctg gatgcggtat
     7741 ttaatcatat tggttcgcaa tctcttcaat ggaaaaatgt cgtcaaaaat ggtgaacagt
     7801 ctgcttataa ggattggttc catattcaac aattcccagt gacaactgaa aagctagtta
     7861 ataagagaga cttaccctat catgtttttg gtttcgagga ctatatgcct aagctaaata
     7921 cagccaatcc agaggtcaag aattatcttt taaaggttgc gacttattgg attgaagagt
     7981 ttaatatcga tgcttggcgt ttggatgtgg ctaatgagat tgaccatcag ttctggaagg
     8041 attttcgtaa ggcagtttta gctaaaaatc ctgatcttta tatcctagga gaagtctggt
     8101 atacatctca gccttggcta aatggagatg agttccatgc cgtcatgaat tatcctttat
     8161 ctgatagtat caaggactat ttcttacgag gaattaagaa gacagaccag ttcatcgatg
     8221 aaatcaatgg agagtctatg tattacaagc agcagatttc agaggtcatg tttaatctct
     8281 tggattcaca tgatacagag cgaatcctgt ggacggccaa tgaagatgtt caactggtta
     8341 aatcagcctt agcctttctc tttttacaaa aaggaacacc gtgcatttat tacggaaccg
     8401 agctagcctt gactggagga ccagatccag attgtcgtcg ttgtatgcct tgggaacgtg
     8461 tatcaagtga caatgatatg ctgaacttta tgaagaggct gattaaaatt cggaaatacg
     8521 cgtcagtaat catttcgcat ggcaagtata gccttcaaga aatcaactct gatctagtag
     8581 ctctggaatg gaaatacgaa ggacggatcc tcaaagcaat attcaaccaa tcaacagaag
     8641 attatctttt agagaaagaa gcagtagcac tagcaagcaa ttgccaagaa ttggataatc
     8701 agcttgtcat ctctccagat ggatttatga ttttctaaaa actagttgat gaagattatg
     8761 gtacatttca taccttatat agtataataa ggctagttac taaacttgta aaggagaact
     8821 taaatgaatt gtagaggaca tgaaacaaga caaagaattg ttagagattt tgaagttcag
     8881 cctaaagcac atattaagct gttagcaaat caacaaaaac atagtgatgc aggagcaact
     8941 attgaagatg aatattatgt atttatcgct gagagtaaaa ttgatggcaa gaaggaagtt
     9001 attcagtgtt gcatgggtgc ggcaagggat tttttagaac taattaatca caaagggcta
     9061 cctcttttta atccgcttgt aggtgattct catgtaaata atagacaaga atatgacaat
     9121 acagggagtg gaaatttata acctgaaaag tggaatgaaa ctgcaaagca gctttataat
     9181 gctataatgt ggttgattat tttatggaat gctaagccgg atacaccttt atttaatttt
     9241 aaagacgaag taattaagta taaaacatat gagccttttg aaagcagtat aaaaagagta
     9301 aatactacta taaagaatgg tagtaaaggg aaaactctga ctgagatgat taatggctac
     9361 agagcggata acgatattag agatgaaatt tgtaacttta atattctgaa aaataaaatt
     9421 cgtgatatga aaaaccaaca aggaaataca atggaatctt acttttagtt attgttgaat
     9481 tttgggtatt ctataaaata tcctaattga gatttaaata gtagactata caatatagtt
     9541 aaaatatcag taaaaacaac actttattga ggtattggat acgctttgct aatagcctaa
     9601 taatcacatg tggagtgttg ctacaacgaa aaaggtgata atccttgatt tcaagctatt
     9661 ttataagcat tttgtctttg tagataaagg caattttgac aataaaaatc ctaaaaggtg
     9721 aatcgttata gatgtatttg tagatatcgt ttgcgcatcg aaaaaattaa tacaagaata
     9781 aatatttata gctctttagg tgacttttat agaagtaaag tttaggatag aaaaacaaga
     9841 aataacgcac catttttggt gcgttatgct tttttatgct ataatggatt tataaaaata
     9901 aaggagtttg ctatgattgg aaagaacata aaatccttgc gtaaaacaca tgacttaaca
     9961 caactcgaat ttgcacggat tgtaggtatt tcacgaaata gtctgagtcg ttatgaaaat
    10021 ggaacgagtt cagtctctac cgaattaata gacatcattt gtcagaagtt taatgtatct
    10081 tatgtcgata ttgtaggaga agataaaatg ctcaatcctg ttgaagatta tgaattgact
    10141 ttaaaaattg aaattgtgaa agaaagaggt gctaatctat tatctcgact ctatcgttat
    10201 caagatagtc agggaattag cattgatgat gagtctaatc cttggatttt aatgagtgat
    10261 gatctatctg atttgattca tacgaatatc tatctagtag aaacttttga tgaaatagag
    10321 agatatagtg gctatttgga tggaattgaa cgtatgttag agatatctga aaaacggatg
    10381 gtggcctaat ggaaatccaa gattatactg atagtgaatt caaacatgct ttagcaagga
    10441 atcttcgttc actgacaaga ggaaaaaagt ccagtaagca acctatagcg attttgcttg
    10501 gagggcaaag tggtgccggt aagactacaa ttcatcgtat taaacagaaa gaatttcaag
    10561 gaaatattgt tatcatagat ggtgatagtt ttcgttctca gcatccacac tatttagaac
    10621 tgcagcaaga atatggcaaa gacagtgtag aatataccaa agattttgca ggaaaaatgg
    10681 tagagtcttt agtaacaaaa ttgagtagtt tgagatacaa tcttttgata gagggaactt
    10741 tacgaacagt tgatgttcca aagaaaacag cacaactctt gaaaaataag ggatatgaag
    10801 tacaattggc cttaattgcg acaaagcctg aattgtcgta tctaagtact cttatccgtt
    10861 atgaagaact gtacattatc aatccaaatc aagcacgcgc aactccaaaa gaacatcatg
    10921 atttcattgt aaatcatcta gttgataaca cacgaaaatt ggaagaacta gctatctttg
    10981 aaagaattca aatttaccaa cgagatagaa gttgtgtata tgattcaaaa gaaaatacaa
    11041 cttcagcagc agatgttctt caagagttac tctttgggga gtggagtcag gtagagaagg
    11101 agatgttgca ggtgggggaa aagagactta atgaattact tgaaaaataa acaattgata
    11161 tttttaggag aatagaaatg agagggttta ataacaagat aaagtctgtt tatcaagaac
    11221 taacaaattc caaagagaaa ttcggtagct ttcacaagac tttaattcat ttgcatacac
    11281 ctgtttctta tgattacaag ctattttcta attggactgc aacgaaatat agaaaaatta
    11341 ctgaagatga actatatgat atattttttg aaaataagaa aataaaagtt gataagacaa
    11401 ttttttttag taattttgat aaggttgttt tttctagttc aaaagaatat attagttttc
    11461 ttatgttagc agaggcaatc ataaaaaatg gaatagaaat agttgtagta actgatcata
    11521 atactaccaa aggtattaaa aagttacaaa tggcagtctc aatcataatg aaaaattatc
    11581 cgatttatga tatacatcct catattttac atggagtaga aattagtgca gcagataaat
    11641 tgcatattgt atgtatatat gattatgaac aagaatcatg ggttaatcaa tggttaagtg
    11701 aaaatattat aagtgagaaa gatggaagtt atcaacattc actgactata atgaaggatt
    11761 tcaataatca aaaaatagtt aactatattg ctcatttcaa tagttatgac attttgaaaa
    11821 aaggttctca cttatcaggt gcatataaac gaaaaatttt ttctaaagaa aatacacgat
    11881 tttggagttt aatattaact cgaaagaatc ttcgcaacaa cttgatattc tctataaaga
    11941 agttggtgta ttaagtttgg gacaaaaagt tgtagccatg cttgattttt tattagcata
    12001 tagtgattat tctaaagact tcagaccatt gattattgat cagcctgaag acaatctaga
    12061 caatcgttat atttacaggc atttagttca gcagtttaga gatgtgaaag ctcaacgtca
    12121 aattatttta gcaacacata atgctacaat tgtaacaaat tctatgacag atcaagttgt
    12181 tattatggag tcagatggag ttaacggatg gattgaatca cagggatatg ttagtgaaaa
    12241 atatataaaa aatcatatca tcaatcaatt agagggagga aaagattcct tcaagcataa
    12301 aatgtctata tatgagacgg ctttatcaga gtagagtcag aaaaagtagg ttagaaattt
    12361 agcctacttt tttctttgtc cgacaggcat agtgtacatc tgaggtccaa gtcctctgtg
    12421 gatatttgct gcagatgaaa ccaatagcga ctcctaagcc tgaatatcgt gaggtagggg
    12481 ggataggaag gaattagcga aatcaaggtt ctacaaacag aatcgtgact tgaagccata
    12541 tatagcggat gaggaactct aaaatccaaa taggtgtcgt aacctatata cgtaaattac
    12601 gagagtaaac taggaaagat gtacggctta ttccgtgagc gtttaggacg tagtacaacg
    12661 aatcatggga gtcagctgaa cacatagtat tgaagaaatt tctgtaatgg aaatggagcg
    12721 aagaagtgaa caattaaatg aatacctctc taattaaatt tgtcaattct aattcctggt
    12781 atgaaaagac agtgacctga aaatgtaaac gatgggagct gatcataaat ataggacggt
    12841 acatgcagtg gtgttagaga ttagtcctta cttgatttgt gataacttcc ccaaatttct
    12901 tctgctatac ttttctcaac ttttaaaaat ccaactaaga attttacctg ggggtttggg
    12961 ggcggagcca ctaagttatc ttatcgttag ctgtcaaaac tggtaggttt tgataggctg
    13021 gcgatatgat ttttgggata ttgtggacac aatatctgag ctcgcaaagc cttacaagaa
    13081 tgaaaatcag ttgttggaaa agtgtactga cattgtatgg tagctcacat tgtcagtaca
    13141 agtattttgg aaaggaagta gcagtatgaa acgagatgtg cgtgatattc ggaaacaatt
    13201 tcgtttaaca gaagcagaag aaaagcaaat tctagctttg atgagagagc ggggagagac
    13261 taatttctct gattttcttc gtaaaagttt actttcctct gatttacaaa aacagatgga
    13321 gacatggttt gccctctggc aatcccaaaa actagaacaa atcagtcgtg acgttcatga
    13381 agttttaatc ttggcacagt cagaacgtca agtcacccaa gagcatgtat ctattctctt
    13441 aacgtgcgtg caggaattga ttcaagaggt tgcaaacacc atacccctca gtaaagaatt
    13501 tcgtgagaag tacatgaggt aagcacatgg aacatcgtta ccgaaccaat ctcaagaaag
    13561 tgtttttgtc tgatagtgag ttgaaccaac taaatataaa tatcgatcaa agtggttgta
    13621 aatccttttc tgaatatgcg agacgaactc tactcgatcc tggtatgaat tttatcacga
    13681 ttgacacaaa cggttaccaa gatttagtgt ttgagttaaa gaggattggc aataatatca
    13741 accagattgc tcgaagtgtt aatcaatctc agttaatttc tggtgaagaa ttgcaggagt
    13801 tgaaaaaagg aattggtgaa ttgataaaag aagttgataa ggaatttaat ctgcaagcgc
    13861 agaagctaaa ggagttccat ggtcatcact aaacactttg ccattcacgg aaagagttac
    13921 cgcagaaagc ttatcaagta cattctcaat cctgagaaaa ccaataatct tgccttggtg
    13981 tcggactatg gcatgaagaa ttttctggac tttcctagct atgaggaaat ggtgcagatg
    14041 tatcatgaaa atttcatcag caacgatacg ctttacgatt ttcgccacga caggatggaa
    14101 gaaaatcaac gaaaaataca cgctcaccac atcattcagt ctttctcgcc agaggatcat
    14161 ctcactcctg aacaaatcaa tcggataggt tatgagactg tgaaggaatt aactggtggc
    14221 aaatttcgtt ttatcgttgc gacccatgtt gataaagacc acctgcacaa tcacatcatt
    14281 atcaattcag tagatagcaa ttctgacaaa aagctcaagt gggactacaa ggtggagcga
    14341 aatcttcgca tgatttctga ccgtttttct aaaatcgcag gtgctaaaat cattgagaac
    14401 cgctattctc accagcggta tgaagtctat cgtaagacta atcacaagta tgaactcaag
    14461 cagcgactct attttttgat ggaacattct agggactttg aggatttcaa aaagaatgct
    14521 ccgctactac atgtggagat ggatttccgt cacaagcatg ccaccttttt tattacggac
    14581 tcaactatga aacaggtggt gcgtggcaag caactcaatc gcaagcagcc ttacacagaa
    14641 gaatttttta agaactactt tgccaaaaga gaaatagaaa gtctcatgga atttttattg
    14701 ctgaaagttg agaatatgga tgatttactt cagaaagcaa aactttttgg actaactatc
    14761 aatcctaaac aaaagcatgt ttcttttcaa tttgcaggag tggaggtaaa ggagacagag
    14821 ctagaccaga aaaatcttta tgatgtagag tttttccaag attattttaa aaatagaaaa
    14881 gattggcaag ctccagaaac tgaggatttc gttcaacttt atcaagaaga aaagttatcc
    14941 aaagaaaaag aacttccaag cgatgagaag ttctgggagt cctatcaaga gttcaagagt
    15001 aacagagatg ccgttcatga atttgaggtg gagttgtcac tcaatcaaat tgaaaaagta
    15061 gtggatgatg gaatttacgt caaggtcaag tttggtattc gtcaggaggg acttatcttt
    15121 gtgccgaaca tgcagcttga tatggaagag gataaggtga aggttttcat cagggaaacc
    15181 agctcctact atgtctacca caaagacgct gccgagaaaa attgttatat gaaaggtcga
    15241 accttaatta gacagttcag ctatgaaaat caaaccattc cattacgcag aaaagcgaca
    15301 gtcgatatga ttaaagagaa gattgcggaa gtggatgctt tgattgaact ggaagtagaa
    15361 aatcaatctt atgtcacgat taaagatgag ttagtgcatg aactagcagc gtctgaattg
    15421 agaatcaatg agttgcaaga acgaatgtca accttgaatc aagtagcaga atatctactg
    15481 gcttcagttg aaagtaagca agaaatgaaa ttaaatcttt caaaactgaa tataactgag
    15541 aatatcagtg ctaatattgt tgagaaaaaa ttgaagagcc tggggaatca actggaattg
    15601 gaaaggggca ggtatgaaaa gatggtagtc aggctagata aatttataaa tcgcttaaat
    15661 acaggacttt caaaaggaga tgggatagat tttcaaaaat aggaatctag ccaataatta
    15721 tatacaatgt gtatcttttg taaaatatat ttgaaattta gatttaatca tgttacaatt
    15781 aacattgaaa tcaaatagaa aaggaagatg caaacgtggg tacactatta gcaacaagat
    15841 taaaaaatag acgaaaagaa ttaaaaatgt ctcagcgaga attagctgag ggtatctgta
    15901 aacaggggca aattagtcga ttagagagtg gagagttcac ccctggagcg gattttttac
    15961 atgcccttgc taagaaatta aaagttagta tggactactt ttttgatgag caggttgtag
    16021 aggaagtcaa tgagttatca gagtttaaga agttagcaca gacatttatc acaaatcgta
    16081 attatgaatc tttaaaatat atatatgaat tggaaaatgt aaaagcccat cgcttatctc
    16141 tagcagataa attttacatg gaatggataa agtctcttat agatttttat ttttatggac
    16201 agaaagaaga ggcaattaca cgattagaga atatgctacc tcagttaaat gtatctgatt
    16261 tgaactacct tcaagtttca aacactctat tcaattttta ttatgatatt gaagatttag
    16321 aacgttttaa tgagattaga gacaagctag agcatcaagt gaatcagctt aatttaaaca
    16381 caattgaaga gttagaacta tcaattaagt tcaattataa cgtttgtcga tacctatggt
    16441 tacagaaaaa tattgaagaa gctattacga aaatcacagc tacgataaag caatgtaagg
    16501 agtacaggac aacttatcta ttagctgatt tgtatttatt gatgggaagt gtaagtgaaa
    16561 atttttcttc taaaagttca gtaaaagaat attttgagac tgctcatttt ctctacaaac
    16621 ttgaggaaaa catgtcaatg gctttgaaag tcgaacatta ttttgcagat ataacagaat
    16681 aaggaatacg gtgttctagt gagcatcgtt ttttaaatct aatttgaata caaaaaagta
    16741 ttaaataaaa aaaaataaaa aaagattata aaaatacttg acaagctaat tttgaagtga
    16801 taaaataaat gcaagaaaac gaaaggagag gtatatgaga agtttgttta ggaaaattgt
    16861 tgctttgtta gtcataggat taatcctatt agggactgct ggaggaactc aagttcataa
    16921 gatggctaga gggattgatc caggtcctgc gaatggtatt tatagatagg agattatagt
    16981 cacttaaaaa tcaaaaaatt ataaaatttt tgttttactt aaatagatat tttatgatgg
    17041 gcgaagtttt tataactagt tcctataagt acagaaacct attttaattt actaatttca
    17101 aaacaatttt ttgagatata tgtaattaaa tctaaattaa gctttaaaaa attattttta
    17161 gcaatttgat tttgaaagtg tcatgcagca atatgaaagc gtagaataga ggtgtttcca
    17221 atgtacaagt aagttttgat tccaaataaa gtaatagaaa aggagctata taatgagtag
    17281 tcaaatgaaa gctttcttga atgaacttca aggaaatatg gaagttgttg gagaattttt
    17341 tgataatcct gaaaaggtaa ttagaaaatt tggaattgtc ggaagagaga aggaagcatt
    17401 gctaatccgt gacttaaatg atatggatga ttatgctctt tcaattcaaa atagcgttgc
    17461 atcgccttct ggggcacata gtagcacttg ccattttgtt agacaagatt tacaatccgc
    17521 ataattgtta atatataaaa cataaaattt caattttcaa ttagggataa tagaattcct
    17581 tactatgata tagaaagtga attaattagg aaatcttagt cagaggtttc tgattaattc
    17641 attttttata tattaagagt gatgtgaaat gttcaaattg tgagactact ctcggattgt
    17701 tgtatagaaa tttaaaattt tattgtgagc tttttaagga agagtggccg atatgaaaaa
    17761 taaatacgat gttaaaagaa ttattccgga tgaattatca gaatcactag atatttttct
    17821 aaaaaattat tctgaaactg gattgtcaga ttataatact tatctctttt atggttttat
    17881 cttgaagtcc tataaattgc ctcgagaaaa tagatatagc ataaaattat tggtgaagga
    17941 attacaaaat agaggactga aagttacatt gattatcaac atttattatc acgctttaaa
    18001 ttgtttggct ttaaatgatg gtctaaaaat ttatggagaa gatttcttaa tatgaattac
    18061 gaaaagtatt taaactattt ggactatgaa actgaaatag aagacgcata ccataattta
    18121 ttattagaat ataaaattag tgataatttt agtgatgaac attggttata taatttgccg
    18181 tctaatatca cccaatctaa gggatttaaa atacacctga gcgcttcaat attaaatgct
    18241 aatttggttg ctaaaaaatt ctttgatttt atattttcta gagagaagaa gataaatttc
    18301 aaaattttgg ttagcattaa agaactttct ttgcaaaata cagggcttaa tggttattca
    18361 caagtcggga aattcattac aatttatcca aaagataata aagagtttca acgtttgtta
    18421 cacaaattag aaatattgta taaaggtgta aagggtgtaa acataccatc agatttcaga
    18481 tttcaactta gcgaggttgt atattacaga tatggggagt tcgtaaaaga tagcactttc
    18541 aaggataaaa gagataagaa aatcccttca aatgtgaatg ttccaataag ggattactat
    18601 attccaagat ataacactat tcctgatcaa tatattattt tggaagtgat ttctaaaaat
    18661 gccaaaggtg gagtttataa agtatttaat actcaaaagc gagtttattc tttattaaaa
    18721 gaagctagtg atttatcgtt ggttgatttt acaaatagag attctgtgaa tcgtttaata
    18781 aatgaaagag aaatattagt agaattggaa aaggaggaat ttactccaaa agtttttaac
    18841 gatttttaca taaagaattc gtattttgtg gaatttgagt ttattattgc agataaatta
    18901 agcgagtata atatggttag tggctcgtat gattggtttt taacattaat tgattatatg
    18961 gagataataa actccaaata taatctttcc tatagagatt tatcatttaa taatatactt
    19021 attgataata gaaataatat ttatattata gactttgagc atgccctaag gaaagaaact
    19081 caaattgaag aaaaaaagtt gcctctattt ggaactccag ggttttatga aaccaactta
    19141 aatttattga ataatcaacc agaggatatt atgggattgg ttttgttatt atattggagt
    19201 cagaatttag atgattttaa cgtcttttca aaaatgagtt ttttagaagc gatggagtat
    19261 gcaatgaatt ttaattcgcg tcgcttagac acacttgata aatcagattg gctttatgat
    19321 atttataaaa aagcgttcaa ttataaatat gatacttttt ctgaattaaa aaaagatttt
    19381 atagaggtat taaaataaag gtaggggtgt tatgaattgt ttcttgaaaa tgaataatgt
    19441 aagtgttcgt tatgatgacg taatagcttt aaaagatata actttacaaa taaataaggg
    19501 agatttcatt ggcttattag gttcaaatgg tgcaggtaaa tctacgttaa ttaattctat
    19561 tgtaggtttt caagagattt atttaggaga aatagagtat tgtgataaag atttgatagt
    19621 tagttctcaa ccttttgctc atttaggctt tactcctcaa accacagtaa ttgattttta
    19681 tactactgtg aaggacaatg taatattggg gctgaacctt gctggaaagt ttgggaaaaa
    19741 tgctgagaag ttgtgtcaaa tagccttaga aattgttggg ttagctgata aaaaaaataa
    19801 tttggtagaa acattgtcag gtggacaact gcaacgcgtc cagattgcta gagcaatagc
    19861 tcataatcca gatttttata ttttagatga acctaccgtt ggtttagata ctgaatctgc
    19921 cgaaaaattt ttaatgtatt taaaagataa gagtttggaa ggaaaaacta ttatcatatc
    19981 ttcacatgac ataaatctac tcgaaaagtt ttgtaaaaaa atactttttt tacaaaatgg
    20041 ctccatatca ttttttggtg atatgcgtga ctttgtagat aattcaacta tcaaattaaa
    20101 tttttcaatg cagaatagaa tttctagata tcaaattgaa tttttagaaa attttagatt
    20161 taaagttcac atcgaagata atgatagttt tacaatagaa gtccctatag aagaaaagat
    20221 cttagatgtt atcaatgagg taggaaaagc atgtgaaatt aaaaactttt caacaagtaa
    20281 attaacctta caagaaagtt atttgcaaag aataggagga gaaaaatgaa ggctgatcaa
    20341 ttaaggcaca aatcggactt aggtttaaga ggtctagcga ttattgctaa aaatgagatt
    20401 attgcttttt ttagaagtaa aggtttaatt atttctcagt ttctacaacc aatcttatat
    20461 gttgttttta taataatagg attaaattct tcgataaaga acattcagtt taatgatata
    20521 aaaacctctt atgcagaata tacaatcatt ggtgttatag ctttattgat aatcgggcag
    20581 atgactcaag ttatttatag ggtgacaata gataaaaaat atgggctact tgctcttaag
    20641 ttatgcagtg gagttcgtcc tttatattat attttaggga tgagtatcta ttctatatta
    20701 gggttgatag ttcaagaaat tattatatat ataattacgt tagcgtttga gataaatatc
    20761 gcaatggata gattttttta tacagttttg ttatctattg ttgttttatt attttgggac
    20821 tcccttgcaa ttttacttac aatgtttatc aatgattaca gaagacgtga tattgtaata
    20881 cgttttgtac taacaccgct tggttttaca gctcctgttt tctacttaat agattctgct
    20941 cctagtattg tgagatggat tggtcagtta aatcccttaa cttatcaatt aactattttg
    21001 agaaactttt attttaaaaa ttcaacaact ttggaattag ttttcttatt gttaacatca
    21061 ttacttgtcc ttatatctgt atcttttatt ataccaaaga taaaattgat actgatagaa
    21121 agataaaagt tgggtcatcc aacttt
//
LOCUS       DBDESG010000043        20764 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.043, whole genome shotgun sequence.
ACCESSION   DBDESG010000043 DBDESG010000000
VERSION     DBDESG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.043"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(493..2220)
                     /gene="ezrA"
                     /locus_tag="ACKB38_001494"
     CDS             complement(493..2220)
                     /gene="ezrA"
                     /locus_tag="ACKB38_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGL7980245.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEEKKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2302..4248)
                     /gene="gyrB"
                     /locus_tag="ACKB38_001495"
     CDS             complement(2302..4248)
                     /gene="gyrB"
                     /locus_tag="ACKB38_001495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGL7980246.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4263..4826)
                     /locus_tag="ACKB38_001496"
     CDS             complement(4263..4826)
                     /locus_tag="ACKB38_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGL7980247.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4911..5465)
                     /locus_tag="ACKB38_001497"
     CDS             complement(4911..5465)
                     /locus_tag="ACKB38_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGL7980248.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5476..6699)
                     /locus_tag="ACKB38_001498"
     CDS             complement(5476..6699)
                     /locus_tag="ACKB38_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGL7980249.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6736..9186)
                     /locus_tag="ACKB38_001499"
     CDS             complement(6736..9186)
                     /locus_tag="ACKB38_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGL7980250.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9386..10315)
                     /locus_tag="ACKB38_001500"
     CDS             complement(9386..10315)
                     /locus_tag="ACKB38_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGL7980251.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLNFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10455..10742
                     /locus_tag="ACKB38_001501"
     CDS             10455..10742
                     /locus_tag="ACKB38_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGL7980252.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10776..12110)
                     /gene="ciaH"
                     /locus_tag="ACKB38_001502"
     CDS             complement(10776..12110)
                     /gene="ciaH"
                     /locus_tag="ACKB38_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGL7980253.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12100..12774)
                     /gene="ciaR"
                     /locus_tag="ACKB38_001503"
     CDS             complement(12100..12774)
                     /gene="ciaR"
                     /locus_tag="ACKB38_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGL7980254.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12883..15429)
                     /locus_tag="ACKB38_001504"
     CDS             complement(12883..15429)
                     /locus_tag="ACKB38_001504"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGL7980255.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15552..15992)
                     /locus_tag="ACKB38_001505"
     CDS             complement(15552..15992)
                     /locus_tag="ACKB38_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGL7980256.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15967..16836)
                     /locus_tag="ACKB38_001506"
     CDS             complement(15967..16836)
                     /locus_tag="ACKB38_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGL7980257.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNILLQS
                     CQEFVKDYLDTICSFYLGKEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16814..17314)
                     /locus_tag="ACKB38_001507"
     CDS             complement(16814..17314)
                     /locus_tag="ACKB38_001507"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGL7980258.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17325..18023)
                     /locus_tag="ACKB38_001508"
     CDS             complement(17325..18023)
                     /locus_tag="ACKB38_001508"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGL7980259.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18016..18252)
                     /locus_tag="ACKB38_001509"
     CDS             complement(18016..18252)
                     /locus_tag="ACKB38_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGL7980260.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18348..19274)
                     /locus_tag="ACKB38_001510"
     CDS             complement(18348..19274)
                     /locus_tag="ACKB38_001510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGL7980261.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19349..20164)
                     /locus_tag="ACKB38_001511"
     CDS             complement(19349..20164)
                     /locus_tag="ACKB38_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980262.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20168..20488)
                     /locus_tag="ACKB38_001512"
     CDS             complement(20168..20488)
                     /locus_tag="ACKB38_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGL7980263.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20578..>20764)
                     /locus_tag="ACKB38_001513"
     CDS             complement(20578..>20764)
                     /locus_tag="ACKB38_001513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980264.1"
                     /translation="VFEVVDETDEVSSKHCFEVVDETDEVSSKHVFEVVDETDEVSNH
                     TYGRATLTWFEEIFEEY"
BASE COUNT         6246 a         4554 c         3684 g         6280 t
ORIGIN      
        1 agcgagaaat atctatccta tcttctagaa tgtcttccaa acgaggaaac tctcgtaaac
       61 aaagaggttt tagaggtcta tttaccgtgg actaaagttg tacaagaaaa gtgcaaataa
      121 gaaatctcca gattaggaac tatccgtgag ttctctagtc tggagatttt tcaatagact
      181 tcgttattgg acggttacca agttactcaa agtacagatt tgaagctgca gataagactg
      241 gcgaaatcac tttaatacta aatgaaaatc aaaaagcaaa ctaagaagct agcctcaggc
      301 tgtacttgag tacggtaagg cgacgctgac gtgatttgaa aagattttcg aagagtataa
      361 aacactgttt tgaggtagtg gataaatcgg ctgggcaaaa actcaatctc aggagaaaat
      421 gaagtaaatc tccccacaac tatagaaaaa gatttgattc tgctcctcac acaataaaat
      481 ctttttcttt tattaaaaac gaatcgtttc acgtgttttc tcatatgagg taacaaagcg
      541 attggttaca ccaggctctg ccacttccaa tgcttgagaa atcttgtcaa atgaagcgtg
      601 ataatcaaat tctttttcaa aaatatctaa agcttcgtta aatgcttctt gaatgcgttc
      661 atcaaatgag cgatagcggt tagaatattg caagagttgc tctgtcaaag ttgcatattg
      721 tacaatatta taagtttccg tttctaaagc ttccatatca ttcgttgcaa tttcaagaac
      781 tcgggtaaca gattcaatgt taatcatttt ttgttctaac tcaaccatta aatcctcggt
      841 attattgctt gccgtaaaga ataacttcaa gaaagtttgt ggaatacctg gcagattgcg
      901 tttttccatg tatcgcttga tagtatggag acgattgaca taaacattgg ccttttgacg
      961 tgcattaata tcatctttct caatttgtgt caggcgctca ctaactgaaa tttgctcatc
     1021 ttcaatatct tttagttgag tttgtaaatc ctcaagattt tcttcaagaa ctgaataagc
     1081 ttgggttggt tcttcttgat ttgaagttac ctcaacaata gctgcctcaa aactctctaa
     1141 ttctgtctga atacgacgaa catggcttgc agctgtctca ggaagtaaat aggtcttgtt
     1201 caaacgtgca atatcttctc ccaataaagt attattctct ttcatatgtt gaagataagt
     1261 tggaagagtt gcaagtagat tttccactac tttctgagca gcaatttctc gagtaaaaat
     1321 atcatacaag gcattgattt cctcttgtgc ctgtccattc tcatattcgg cattatccaa
     1381 ttccaactga cgaatattct cttggttttt cttgaatgct tcataaagca agtggaaacg
     1441 cgcttcaata tccgtttcaa caaaatgata attagcatca attagtttac gataaccggc
     1501 ttccaaatcc tgtaattgat ctggcaattc tgtagaaagc gtcgtaacca aggctggaac
     1561 acgatccaca atatgactta aggccaaaat gtgattttct gtattatcca aaatcactgc
     1621 ggcttccaca gggtcacccg atgaattcaa ggttacaaat tgtgaaaatt cagattggat
     1681 attttctaat tgtttttcaa tttcatccaa ggcttgacca tactgttctg aattttcagc
     1741 aactctatgc tgaagttcct caaataaatc caaagcatga agaacacgac cactattttt
     1801 agattcttgc ttctctaagt ctgccaaagc attgcgaatt gccgcaatat cttcttcaat
     1861 caaagtaatt tgactctcaa tttggtcaat ttgatgactg gccttgagaa aacgaaatga
     1921 atggttatag ccttctgctt caaagagatt attttcaata tcggcaaaag agttgagaga
     1981 taaatcgacc catttttgat tccattcacg gaaagccact tgactttgtc caatcaagtg
     2041 catatttttt acagcttcta cttcatcatt tactggaaga ttgtatagtt cttctttttt
     2101 ttcttctagc gcctctaatc tcccctcgtt tcgcttacgt agaaagattg ccactacata
     2161 agccagaact aaaatgactg caattgcaac cattaaataa attagttgtc cattagacat
     2221 atcaaactcc ttttttactt gaaacaatcg taacgattat atcatatttt ttagaccaag
     2281 ggaactactt ctccctattt tttagacatc aagtgtacta tagacagcat tttcttcgat
     2341 aaactcacga cgaggctcta ctcgatcccc catcaacata tcaaagattt tatctgcttc
     2401 tgcagcatca tctacagaaa ctctagccat caagcgatgt tcgggatcca tggttgtttc
     2461 ccacagctga tgatcgtcca tttcacctag ccccttataa cgctgaatag tcggtttggt
     2521 acgaccttca ctataacggg ctaaagcttc ttggagtttg atttcttgat ctgcacccgg
     2581 ctggatatat tctttaatct cgcttccaac cttgacacca tagattggtg gttgggcaat
     2641 ataaacataa ccagcttcta ggattggttt catataacga taaatcaagg ttaaaagaag
     2701 ggtacgaatg tgggctccat cgacatcggc atcggtcatc aaaacgagtt tttggtaacg
     2761 ggctttcgaa acatcaaatt ctgcgccaaa tcctgttccc atggctgtga aaagactacg
     2821 aatttcttcg ttggctagaa tcttatccat acttgctttt tcaacgttca aaatcttacc
     2881 gcgaattgga aggatagcct gaaactcacg gttacgacca gatttggctg atccaccagc
     2941 tgagtctcct tcgacgatga agagttctgt ttcagcaggg ttattagaag aacagtctgc
     3001 tagtttccct ggaaggttgg aaatttccaa accagatttt ttacgtgtga cttcacgcgc
     3061 acgcttggca gccacacgag ccttggcagc caaaattcct ttttctacga tacgtttggc
     3121 aatctgtgga ttttccatga ggaaatcgga gaaagcttca ctgaagaggc gattggtaat
     3181 cttgaccact tcgctatttc ccaatttggt cttggtttgt ccttcaaact gtggatttgg
     3241 gtgtttaact gagataactg cagttaagcc ttcgcgaaca tcttcccctg ttaaattatc
     3301 ttcattgtct ttcagtaact tatttttacg agcataatcg ttgataacac gtgtcaaggc
     3361 tgtacggaaa ccttgttcat gtgttccacc ttcatgggta tgaatattat tggcgaaact
     3421 catgacattt tcatggtaac cagttgtgta ctgcatggct acctcaactg tgatatcatc
     3481 catctcaccg tctgtataga ttggtgtatc aaagattaca tccttgttct cgttgatata
     3541 ttcaacgtaa ctagcaatcc caccttcata atgataatgc ttggtttgtt ccaaaccttg
     3601 gcgcttatct gtaattgaaa tttgaagacc gcgatttaga aaggccaact cttgaatccg
     3661 tttatttaat ttatcaaaat caaagattgt tgtttcagtg aagatttttg ggtccggtgt
     3721 gaagtgaaca gttgttcctg ttttatccgt atctccaact atttcaagat ctgcgacaac
     3781 atgaccacga cggtattctt ggtaatgaat cttaccattt ttgtgaacat gaacgtctaa
     3841 ttgagtggaa agggcattaa ctactgacga ccccaccccg tgaagaccac ctgaaacctt
     3901 gtatccacca ccgccgaact ttcctccagc gtgaaggact gtaaagacgg tctcaacagc
     3961 aggacggcct gttttttcct gaatatcgac tgggatacca cgcccatcat ccacaacagt
     4021 aatcgaatca tctggctcaa taaaaacttg aatatggctg gcaaatcctg ccaaggcctc
     4081 gtcaattgag ttatcaacaa tttcccagac tagatggtga agaccttctt ttgaggttga
     4141 tccaatgtac atccctggac gcatacgaac agcctctaag ccctctaaaa cttgaatttg
     4201 actggcatca taatcctgtg cctgcagatt tttgatttct tctgtcatct tattcctttt
     4261 tcttatatgt ctaatacttg tcttaaattc acgatactgg taaacaagtg ggtatcaagt
     4321 cctgcagctt gacctgcttc gatatcaatc ggccgatcac caatgacaag accagagcta
     4381 atctgatact tttctcttaa ataaagcatg gattcgggat ttggctttct cttaaagcct
     4441 gagctagaag tcaccacttc tgtaaaataa gctgctatag aggttttttc taaaatttcc
     4501 aaaacctgat catttcgatg agagaccaaa aaatgacggc caccttgatt tgaaatgtct
     4561 tccaataggt cagaaactcc ttcaaataaa atcgggtgtt caagctctct ggcttcattt
     4621 tccttgtact tttctaaaaa attctctaaa ttgggagcga atgtctcaat cgcaaaagga
     4681 gtagaaacct ttaaagcttg atagacactg tcatggtctt gtgtgatacc atacagtgcc
     4741 aatgtttcaa caaatgcagc tgttgaagtt tcataattat ccagtaaagt tccacctaaa
     4801 tcccagatgt aatcgtgata tttcataccc ttcattatag cattttttta tgaaaaaagc
     4861 gatgatttcc ctgagttttc cacataaaaa acgagagaat aactcccgtt ttactgattt
     4921 ttaccaaaga catctcgata gagcattcct gttcgtaaac tctctgcgtc ccctcctagt
     4981 tgctccacca actcgtaggc aaaggcaagg gctgttgaag gaccccgact ggttgtcaac
     5041 tgaccatcta ccactactgt ttccttgacg tagtgaccat caaggatttg ctcttgaacg
     5101 ccgtcataac aagtgtatcg cttatttttc aatatctctg cttgattgag ggcaattggt
     5161 gccgcacaaa tggctgctag tttcttccct tcttgctcga agctttgcaa ttcttgaatc
     5221 aaggtctgat tatcacgtaa atgtgcagaa ccaggcatac ctccaggaag aacaatcata
     5281 tcatagtctg ataaatctcc atcaaagaca tgatctgctc ttacttggat tgcatgcgaa
     5341 cccgttactt gctcttcaaa accaaccata tcacatgtga tattggctcg acgcaagaca
     5401 tctacaactg tcaaggcttc aatttcttca aagccctgag ctaatataac tgctactttt
     5461 accatgattt tctccttatt taatttgttt taatacaacc tttttccgtt tgaatgggac
     5521 ttttccgctc ttttcttcag ctagattagt ctggtaactc atcgataaaa gcaaaccaac
     5581 accaatcaga ttactgataa tagctgatcc cccttgcgaa atgaaaggca agggaatccc
     5641 cgtcaaagga agtagtccag tcacagcacc gatattctca aagatgtgga agagcaacat
     5701 cataatcaaa cctgtggaaa tataagtgta gaactggtta tttgatttaa gagtaatctt
     5761 caacatacgg taaatcaaca tgagatagag ggcaataacc aggacagagc caataaagcc
     5821 aaaatcttct gcaataaccg taaaaatcat atctgactct cgaactggga taagcagatt
     5881 cgaagcatta aatccctgac caaataagcc accactccca atggcaatct gcccttgagc
     5941 ctgctggtaa gtcgttgttt gggcaaactc aaagggattg agccaagcca aaatccgatt
     6001 aatttggtag gtcggcattc caatctggtg aagaaaagct cgtccgtcct tgctaataaa
     6061 gatagctaag aaaccagcaa ctcctgttac agcagtcaca aatactggga taataatttt
     6121 ccaagaaacc cctgataata aaacgattcc tgagaaaatg gctacaaaaa ccaaagccgt
     6181 ccccaagtca ctttgaagtg ctaaaagaac taggactgga atggtaaaga gaatcatcca
     6241 gaaaattaac aaaaagtcca gcggaaccgt gcgtctccat tccttatgtt tctttgtaaa
     6301 ttggacaatg acacgagcca acatgaggat ataggatatc ttcataaatt ctgacggttg
     6361 gaatagggta attccattta ttgataccca gtttttggca cccgttgatg caactaagct
     6421 tggattataa aatacaatcg gcaagatcat aagtcccaag cctaaaatat atagaaaggg
     6481 ggtcaccttc caaagaaatt ctgtattaaa gagcatgacc acaaaaccaa tcacaagccc
     6541 caaggcaatc caggcgacct gctgccctaa aatgggcaga atattattgg gataatcatg
     6601 actaacggct atatagatag ccaccacacc gatgaccagt agaaaaaata ctggcaagag
     6661 caaactgtaa tcgactcttg agtcgagaga acgtttcata tatactaacc ttatactttc
     6721 atacaatact atttatcaaa gctcattaaa aaatctatca atagcctcgt caacttcgga
     6781 tcgagagatg gttttaacag tcgcttcttc tgctagagat gctactattt gtttgccgta
     6841 tcgttttccg acgattctcc tatccaaaat aagagttaag gaacgttggt attcacgtct
     6901 catacttctt cccaaagcct gttttaaacg aataatggcc attggcaatt gataatcata
     6961 aaaggcattt ttcccttctt gattcagttc ttgattaatc tttttcgtca agggttcttg
     7021 aggattttgg aaaggaagcc tcggtacaac ttgaatcaca gaaggatggc ttgaaaaatc
     7081 aactccctcc cagaaacttg ctgcaccaag caagatttgt tgttcacctt tttcaaagcg
     7141 tttctttagc tgatgaacat ccccattttt atactgggcc aagtggctaa ctgtaagtaa
     7201 atccgatact gctagaagca tgtctttagc ggtaaagaga accaaaatcg gttgctggaa
     7261 agcttgaatt tccactagta aagcagctac ctctctggca tagacttcta aggaggtttc
     7321 tgttaccaga gggaaatctt tgaccatgac cacttcttgt tcctgttttc cccgagattc
     7381 aatcttgaca aatttagctt caggatagcc taaaaggtct gccaaagaaa ccctctgact
     7441 aatctcaaga gtagccgata ctcccaagac ttggcaactc tcaggaagta aagaggacag
     7501 aagagtacgg cctgattttg tagaagaaat ctgaattttc ttttgactcg tttcagttac
     7561 ttcaagccag taatcacctt cagctgtaaa atagcgaacc agctcatcaa agtcttctac
     7621 ttccaattct gaaaaatact gatggagatt gtccagagaa tctaagatat ttttcctaga
     7681 tttgccagac tgaaattgtt ctatcaagta gagacactca aagcgaatac tttctagtat
     7741 ccgttgttga accctgtttt cttctcctac taaagcctta tcaattaaat cgataataga
     7801 ttgtatatcg taggtctctt gaagcagatt ttctagagct aacaaaatct tttggacttc
     7861 atcaataatc agtaaacggt cactgacaaa ttcaggatta tcttcaagtc tggttacgag
     7921 ataggcatga ttagtcacta aaagcttgca agtctctgcc ctttcttgac tacgtttcca
     7981 aaaatcttcc gtcacaaata agctctggga tgataaattc ccatcatgac gaaggtctgc
     8041 tagaaaatgt tggtaacggt agagttgccc gatttcatcc aaatctcctg tctctgtctc
     8101 agtaagccag accaagactt gcattttaaa gcgtctaaat aagcgatttt catcattttc
     8161 ctgcaaggaa tgataaaagg catccaactt cagataattt tgtggtccct ttaagctatg
     8221 aatatctgta tggaacactt ccttgaggcg tttaccttct tcttccatga tttgattttg
     8281 aagaatcttt gtcggaacac taagaacaat ttgtcgctct ttggattgag ataaagcggg
     8341 taagagatag ccataggttt tcccaatccc tgtcggcgct tgaatcagag agacaggttc
     8401 atctttcaat agcaagccaa cctctttagc aaaactttct tgttcctccc tcacttcaag
     8461 gttcaacaga gaaatatttt tagaaaagtc ttgagatagt tttcgtggct ccagagaagc
     8521 ttccgttttc ttaaaatata gaccttgaac ttggaccaag tctggagaac tcaggataga
     8581 ttggttgcga taagtttcct caataaccag gtaggactca tataggagag cgtcagccat
     8641 ttccagcaag cgttccaaga gacctttagg aagctgggtc atctttttcc gtaaaaaaag
     8701 aagtaattct gctgtagctt gggcatctga aagggctgtg tgtgcgtgtt taagaggaat
     8761 tcctaattct cgacacaaaa tcggcaagct atatttttcc agttcaggga aaaagacctg
     8821 ggccaattcg accgtatcaa cacgagggtt tcttagctca tagccttcaa aaaataaatt
     8881 ttccgccaag agattagcat caaactgaac attatgggct acaaaaatcc catcctccac
     8941 caagtcaaat atttttctgg caacttgcga aaaatcaggt gcttgcgcca gacgttggtc
     9001 tgtcaatcct gtcagttctt tgatatgagc atccaagggt tcatgtggat tgacatccgt
     9061 cgtatagtga tcgacgattt ctccgtcctc aatcacgaca attcccactt ggataatttt
     9121 agccttacta cctgtgctag ttgcctctaa atccaccaca acatagcgat taccaatcgt
     9181 tttcattata aaaaattata ccaaaaaaag ggccatttgg cacctgcaaa cataggataa
     9241 ttttcaaact caagaacact acttttctta aaaatcccat cgaatagagc attctagcct
     9301 gctgattttt gtaaaatagt gaagaaatac gaaaaattga gagaagaaat gtttgcatct
     9361 tccttctctc aacgaatcat gtattttatc taaccatgtc aggatcgtag tcataaaatc
     9421 ctgtatcaag gaaacggttt ttagggtgaa gtttacgaac ttcttcatcc agcaagaagg
     9481 cttgagcatg actaaagttg ccctcttgaa tcaagctacg cgcttggata aagagttttg
     9541 caatctcaac aaagttctga ccaggagtgt agagcccttc tagtttccag cgagtaaagc
     9601 catgctccac caattctgtt aatttgatca tcaaatcaag gtcattgttg gcaaagatat
     9661 gggtcccatg attatcttca aaaatggaat agtggctctc tggatcactt ggctcagcca
     9721 agaagaggtc acgtttatgc gtcttttcat catcgatatg tgtaaagtta tagtagtttt
     9781 gcaagagtgg acgtttagaa tgatggatga cgctagcacc gtaaaccaaa acttcagcag
     9841 gaatttccaa aatctctggc attttgaaaa gttcagctga tggaatttca cgcgccaaaa
     9901 cagcctcaga tgcgccagcc ttttgtcccc agaagttaat ctgacgactg ctagttacca
     9961 tggttgaagc atcgtagatg gtcttaaatg aataaccatc gcggttaact acgtaaaaga
    10021 cgcctgcatc cccaatcgta atatagtctg tcttgatttc ttccaagaag tttaagaaag
    10081 gcttgatacg gtccatcata tcttggtgca tgagagcatt gaccgcaacg atcaattcct
    10141 taccagcatc atgaaccaac ttagcgattt cacgtaattg gtcataacta aaggtcgttg
    10201 gcagacgaag accaaaatct ttctcaccga catagatacg gtctacgcca gcttcgagta
    10261 gttgttcaac ttgttcaata ctttcagcag ttgctgtaat gacaatcttt tccataagaa
    10321 aaattatacc acatttctcc aaaatcagcc atgctttgaa aatgaaaagg gactttattc
    10381 tttttgtaac ctttctgtaa taattctctg ccgaaaaaat gataaaatag gtatgtactg
    10441 ttaaggagag aatcatgccc gtaagaaaat tacaatccta tgaggtagac tatcaagaag
    10501 aattaaacca gcagcttcct cattaccaag cttatacacc tgaagcgcaa tctgatgcca
    10561 atctcaagga aatcctattt tttattaata tcgctgtttt ttgtatctgt attgctatct
    10621 ttagttttat ctttttagca ttaaaattat caactgctct tgcctttgcc gcagcaatcg
    10681 gattcagctt acttgtttta aaagttcaac ggtctattat caaacgaaaa cgtagaagat
    10741 aaatccaaaa tatcgcccca attggagcga tatttttatt ttttcttttt agatggtgtc
    10801 tgaatggcaa tcttcacttc aaagattgtt cccttgggtt tattatcttt gacagtaaca
    10861 gttcctttta gagcatctac aatttgcttg gctagggata atcctaaacc aaaaccacct
    10921 ttttgccggg ttctagcctt gtctactcga taaaaacggt caaaaatttt ctttttatct
    10981 tctgtcgaaa taccgattcc attatcagaa acaagtaaat aaagattgcg atcggtcgcc
    11041 gagataagaa aatcaatttc accatcctcc tcagtatact tgacggcatt atcgaaaaga
    11101 atggtcatca gttgtttcag aagaagctga tctgtgacaa ttgttcgatg gatacgattt
    11161 tcaaaacgga agacacgatt attttccgaa gcaatcatct cgtagtttgt gaaagttgta
    11221 ttaaaaaagc tagttggaac ttctgcaagc tccggcttaa tcccatcatc tctccgagct
    11281 aagttcagca agcttgtcgt taaaaaacgc atatttcgga cttcttccaa actcgatgca
    11341 atgctttcgc tcacatccat aatggtagct tctggcttac gaaaaagggt ctctaagcga
    11401 ttttgcaaaa ctgcgagtgg agttcgtaac tcatgactgg cattttccac aaaagactgt
    11461 tgcttctgca tactctcaag caggggccta acactgaccc tagctagata gagactggca
    11521 agtaaagaca aaatccagaa actagccatc acgaccacaa tcaattgctc atgcttttga
    11581 ctagcctgtt ccaactgact ggtattaatc aagacagcag catacttgat attggttgaa
    11641 accgaactaa tattggtctc catcaaaatc acacgataga tttcttcctg cccatagcta
    11701 ttaaaaacct gaatctggta gatatgtcct agttctttct tttctaactt aatcttatcc
    11761 aagcccaaaa atcgatttcc agaaagaagt tgagtaaagt ctttatcaaa gagaatgact
    11821 tccgtattgg aactgacatt aggttttatt tctactttac tagcgtccgc cctagcattt
    11881 tctaaatctt taatctcttc tgttgcccta tttatagcca gctgaataac tgcttgagga
    11941 ttttcactca gtccatgaag cttatcgtcc accgaagtat aaagactcga atgcatgact
    12001 tgtaaaataa tcagagtcat tgtagaaaaa atcagggtga agacaccgaa gttgcggata
    12061 aaataactaa agtcatccgc ataccatgtt tttttaagtt tactgaacat cttttaaaag
    12121 atacccaaca ctacgcaaag tttgcaaatt ctctgcaaaa gtggttccct ttaatttctt
    12181 acggactttt gaaacataga cttcgacaac cgaaatcgtt gtatcactat caaatcccca
    12241 tagacggtca aaaatctgcg tcttaggcaa aatcacattt tgattttgaa ggaaataaac
    12301 tagtaaatcg aactctttcc ccagcaattc gacaggagta tcttcaactt taacggtatt
    12361 ggttgataaa ttaaccacga tattcccata agtcaaggtg ttttcattaa acttccctga
    12421 acgtttgaga agggcctgaa tccgcatttt aagttcttct aggtagaaag gtttggtcag
    12481 ataatcatcc gctcccagtt caaatccatg tcccttgtca tccaaacttt ccttggcagt
    12541 cataatcaga actggtgtcg taattccctt ttcacgcaat tcttttaaga cttggaaacc
    12601 atttttttct ggcaacatca aatccagcaa aatcaagtca tagacaccac tctcagcttc
    12661 gtagagacct tcttctccat caaatacctg cataacatcc gcaaaatcgt ctaaaaagtc
    12721 aaatactgaa tttgacagac ctaggtcatc ctcaaccaat aagattttta tcatgagaaa
    12781 ctcctcctta ttaaaactat tataccaaat ttgccttaaa aaaaactcaa ctctctgcat
    12841 tttacatgag atagctgagt tttcttttta ttttaggctt atttatgcat ttccgtattg
    12901 aagaacaact gcttcgactg cagctttttc acggctaatc aagtcaacac gcgctgcaat
    12961 ttccttgatt cccataccga tgttacggct aagagcaagg tcagaaagtt gcggttcaaa
    13021 gaactccttg tattccgcca agcgttgctg agtcttaaat acatgagcag gaaggataac
    13081 aaagctatca aagctcatat ctcctccaag ggctgcctta atccaagccc agttttcacg
    13141 cgcccaagac caagctgttt tctgagttgc ttgatgagct aggaattggt aataccaagc
    13201 agacaagtcc tgtggtttga ccacaaattt gtccttccaa gaagtaatca ggttttggat
    13261 attatccgca tctgtactgt atgcaagagc tgctgccaac tggcgtttaa agacagcatc
    13321 tgttgcgtga gtataagtat caagataaag tgctaacaag tctttagtct catgatgttt
    13381 catctcatta atcagaactt gtgagcgaat agctgctggg agtcctgcaa gattctcctt
    13441 gtgtgttgcg aagatttggc tagcgacttg actagcttct gcatcatttg agcgaatcat
    13501 catcgaaaca gccagctgac gaaccaattc atcctcatct gattctccgt ctttagcttc
    13561 aaaaccaaga cggtcatagt tatgacgagc caatttagca accagtcctt tgaaggctgt
    13621 ttcagcatcc gttccttcat caataaagcg ctcaagggct gaaatcactt gagaaacagc
    13681 tgaaaccacc agataagact cttccttagc aagtttatca agaactggaa gcaagtctgc
    13741 ataagaaatg tgccctgcct cagccaacaa acgacgttct tgaacaattt gcagtttgct
    13801 tgtgttatca agtgtctcta gctcagcaag aacagctgct aacaagtctc cttgatagtc
    13861 ggtaatatag tgggcagtat tttcagtgtt gagacgaaga gctccttcat tttcagcaag
    13921 aagagctgcg tagccaggga tttcgatact ttcagtttcg agtgtatcag gcaagccttt
    13981 ccagttgcta ttgaggggca ccacccagag acggttcttg tcttcgttct caccgatgaa
    14041 gaattgtttt tgtgaaatct tcaagacatc attttcaact ttaacagtaa gaactgggta
    14101 accaggctgt tccaaccaag aatccatgaa ggctgcgaca tcacgtcctg acgcttgacc
    14161 aagggcatcc caaaggtcac taccaatggt gttgctgtat tggtgttttt caaagtaggc
    14221 gtgcaaacct ttagcaaaat cagcatctcc tagccaacgg cgaagcatgt gcatgagacg
    14281 gcttcctttg gcatagacga tagcgccgtc aaagagtgta ttgatttcat ctggatgttt
    14341 aacttcgacg tggacagact gaacgccatc agtagcgtca cgttcaagag caagaggtac
    14401 tccacctgtt tggaaatctt caaagatatt ccagcttggt tcgatggtat ccacacagac
    14461 gtattccatc atattagcga aactttcatt gagccaaagg tcatcccacc atttcatagt
    14521 cacgaggttc ccaaaccatt ggtgagccaa ttcatgggcc acaacaaggg caacttgttg
    14581 acggctagca aatgtagagt tctcatcgac aaccaagtaa acttcacggt aggtcacaag
    14641 accccagttt tccatagcac cagctgagaa gtcaggaagg gcgatgtgga gagattgagg
    14701 aattgggtac ttaactccat agtaatcttc gtaaaactcg atagagcgaa cagcgatatc
    14761 cagtgagaaa tcaagatttg aaagtggatg tgctttggtt gagtagacac ctaccagggt
    14821 accattttta gttttagcgg tcaccccttg caaatcacca gcaacaaagg ccaacaagta
    14881 agaagacatg cgaggtgttg tctcaaactt ccagatacct gtttccttac ggttttcaac
    14941 atcgatttct ggcatgtttg acaaggccaa ttcaccttct gcttggtcaa agcgaagaga
    15001 gaggtcaaaa gttgctttgg cttcaggctc atccacacat gggaaagctt cgcgcgcaaa
    15061 atggctctcg aactgagtag acaagacctc cttcttgact ccatcaactg tataataaga
    15121 agggtaaatc cctgtcatgt tgtctgtaat tttaccagaa aaggcaagaa ccaattcaac
    15181 ttgaccagcc tcagccaatt cgatatgaag ggcttcattg tcatggtcaa ctgtaaatgg
    15241 acgagcttga cctgcaactt ctacagaggt gatttccaaa tctttttggt ggagggagat
    15301 gcggtcactc tgtgcttgac cagtgatggt cactttccca gaaaaagtct tggtctcacg
    15361 actcaaatct aaaaataaat cataatgttc aggaacaaat tgcttaataa aatgttcaac
    15421 tgcttgcata gttttctcct attctaagtt taagagctgc tgctcttctt caaacaaact
    15481 tattatatca tattttgctt cagaaaaaag gattggacag cgatttccaa aactttcttc
    15541 cttctaatag tctacagtgg gtagaccttg cgaaattctt ctaaaacaac cttgctttca
    15601 ggtgtaaaag tcaatcctgc ttcacccaag cagtctctga aaaaatcctc atgaacctgg
    15661 cgccaataga caagtgtctt gtttccctca ccttccttat aggcatggtc agctgacact
    15721 cgattgaagg gtttaacaga aacctttgta atttcgacaa tgcagacagc ctgattttga
    15781 ctatctaaaa tgacatcgaa ggtccctact tggggaagtg gttcgtcttc tagcacatag
    15841 aggtcatagg ctgatgctgt tgctgtcttt tctcctttaa acaccaaatc cgctaaaagg
    15901 tctggttcaa ctccaaaagc ccaggcatcg atttcatctc cgatcaaagg attgatttgc
    15961 ttgtatttat tccacatttc ttgcggtatc atgggtgctc ctttgtaatt ttttactttc
    16021 ttcttttatg tgtttaagat gatctggatg gtcaatctct aaatcaaaaa tctctggaat
    16081 agaactgtag tggataatgc acttgatacc caactgattc attttttgta tgaaagaagt
    16141 attcagatag cctgctacag caaaatcaat cttgttcttt cttgctttat cctgcatatc
    16201 tcttagcata tctaacatta ttggactttc catatcatgc cattgactgt ttctcatagt
    16261 cgcaaaaaca aaggaagtca aatcattcat tccaactaca atctttgaaa tgcccgtttc
    16321 cagtatacta gataagtcaa aatacgctga cggtaattca atcatcgttc cgactttccc
    16381 agtaaaaccc tgctgacgca atactgtaat agcttgtttt aattggtcgg catcattgac
    16441 aaaaggaaag ataacagata gattggggtt ggtttgataa acttctgtaa cgacatgtgc
    16501 ttcagcctga aattcatcca aacacgccag taaacgccta gttcctctat agccaaacaa
    16561 gggatgccct tcgtcaaaaa actctttagt ccccactaaa caattggctt ctgtattcgt
    16621 taattcagta aaacgatacc aaacttcctt acctaagtaa aaggagcaaa tagtatcaag
    16681 ataatctttc acaaattcct gacaactttg taatagtata ttttgattga gctctctcaa
    16741 taagtattcc ccacgaatca tgccgacgtg gtgaaatagt tgaggataaa ttttttcaag
    16801 aattttttcg ccactaaggg caagttgatt tctcatcatt caccttccaa ttcatgtaag
    16861 aagtcttgtc cagttctgga aatcctaata attcagactt aaccttcaag actaatggcg
    16921 atgcattttc ttctgtaatc tcttgaatat ccatccaaat atatccaagt gaatcattcg
    16981 caccatcaga cacagcttcc gaaatcgtaa cttgaggtgc actctcattc atttcaacat
    17041 catacaaggc tatgacatgg tgaaccataa aattttttaa ctcttccctg acgaaaacat
    17101 cgtagattcg aggattagag tagcttctaa cagtaaatcc cgtctcttcc ataacttctc
    17161 tagtcagcgt ttccgtcagt ccttcaccaa gttgctgact gcctccaggt agatcatacc
    17221 gatgttgata agggcctctc gttttttcaa tgcaaagtaa ctttccattt tcaaagcaaa
    17281 cacagtagac cccaaagtga tttttgattt ccatccaact cctcctactt caaagaccag
    17341 ccaccatcta ttgtcaagat ttgtccttgc atggcgctcg cttttccact tgctaaaaaa
    17401 agactaagct ctgctatttc ctctggctca atccagcgct tgattggggt ttcactagcc
    17461 acccagtcag ccaaaccacc tggttcaaaa tccgcagcgg tcatagctgt cttgactgct
    17521 cctggagcga taccaaagac ctgaatccca gcttcagcat agtctagagc caactgcttg
    17581 gtgaagccag ccaaggcatg cttggatgaa gtataggcgt gaccacctcc acctgctagg
    17641 ctagaagcaa tggaacacat attgatgatg attccctttt tattttccag catttgtgtc
    17701 aaataatacc gagtcaactc tactggaata atgtagttga tttcaaaaat ctcttgaatg
    17761 tcctgcgccg tttgttccaa cagtggtttg taatcatcca aaactccagc agtattacac
    17821 aaaacatcca cctgagggca ccagtcaaaa ataggttcca agtccaaggt caaatctctc
    17881 tgtaaaaagc gaaaatcacc ctctaagagt ggcttttcac cttggtcaac tccataaact
    17941 tgatagccct tctctaaaaa gaggcgagct tgagccaatc cgatccctga actcactcct
    18001 gtaatgagta cacgtttagt catgcacttc tacccaatcc gttgccaaaa catcacaaac
    18061 tgtcgggctc cacatggaaa aaccttctcc ttcgccagaa acgttgatta ggaaataagg
    18121 tgtcatttca agtgcaagcc cattttgctc gatggtatca aagagttgga catagttttc
    18181 cgcacctccc caaccagttc gtacatattt tctcttagcc tttaacccag gcaggatctc
    18241 ttcaaatgtc atgtttttct cctttaattc tacattcttc atttaattat agcaaaaaac
    18301 cgctttatac ggctttttga atgtgagtta ttcaaacctg ctactactta cggcaaatta
    18361 ttccctgcag caagataaat ttcataccat tcttttcttg ttaagctaaa gtttgccgct
    18421 cggctaactt ctctcaagtg cttaggattt gttgtaccta cgactgcctg catttttgct
    18481 ggataacgca atatccaaga aatggcaata gttgaagagg ttactccata tttaatagct
    18541 aaacgatcaa gtacttgatt taaagcttga aatttctcat ttccaacaaa attcccttta
    18601 aaatacccga attgtaagac agaccatgct tgaatgacca catcgtgtaa ttggcaatat
    18661 tcaaaaatgc tgccatctcg catagctgct tgactatctt ccatattaac atgaaaagct
    18721 gattcaaatc ctggagtaaa agccgcactc aattgtagct gattaacagc taacggctgc
    18781 ttgacatctt ttttaagcaa ctccatcatc ataggatttt gattagaaac tccaaaatct
    18841 cgaactttac cttgtttata aaggagatta aaggcttctg ctacttggtc agattccatc
    18901 aaagcatctg gtcgatgaag gagcaagcta tctagatgat caatcttcaa tctttgcaaa
    18961 ataccgtcta ctgattttat aatatagtcc ttagaaaaat caaaataggt aaattcttca
    19021 atgcgaatgc cacatttgga ctgaatccac atcttttctc ttaaatctgg acgatttttt
    19081 aggacaagac ctaacagttc ttcacaacga ccacgaccat aaatatcagc caagtcgaag
    19141 gcattgattc caacagaaag tgctgtttct acaagctctt caacttcttt tacagattta
    19201 tcttttattc tcatcattcc gagaacaatt tctgataatt ctttgtcatc ttgaccaaga
    19261 gttatgtatc tcatcaaatt tttctccttt aatttctaac attcttccct tcattataac
    19321 aaaaaaccgc tttgcaacga ctttttgact atacttcact ccattttatc ttcttaaacc
    19381 cacggaacaa gacaaagatt ccaataaaga ggacagctaa aggaataact tttgtaagga
    19441 aaacatttga aattcccatc cactcataat aacggagcag agaacccacc acaagatggg
    19501 caataatcat actgacaaat ggacgaaaga ccgcttcttt ccaattccaa ataccgataa
    19561 ctagcgaaat cgtaaagaca gacaaactat cccagggagc cggaatataa aaggctcctt
    19621 cttgtatgaa gcttgccatt cctacatatc ctaaaacaac tagaagaact atagtcccaa
    19681 caacaatgta agtgccaatt ttcattttag gagaatcttg gactaaactt cttcgtaaaa
    19741 ttgtggccac aagtccaaat ccaatcagaa aaataagaag ttgccctaaa aatgtgagca
    19801 aattgactgt taagagagga cctttagaaa aatcacttag tagttgataa taacgtaata
    19861 ccgccaggac aagaattggc gtcaaaaggg actctttgat agaactgcga ggtgctccct
    19921 tgagaatctc tttcattatt tttttaggat tcttacctag ataatcctct gcactcatgc
    19981 catctcgttc tgcttctgag aaatctagca tcatcaaata gatctgctct ctgagatagt
    20041 cttcatcata gagaaatcca gcaagattaa aactttccca caactcctca aaatactttt
    20101 gattctcctc agaaaactca tgtagcaaag cgcttgtttc ttcgtaatac ttcattttct
    20161 tcatggttta acccccattc ttaatccctt ctactttttg actcaaatcg tcccattgtt
    20221 gccaaaagac tgagacacgc tcttctcctt ctttcattaa tgaaaaatac ttccgatctg
    20281 gaccatctgg cgacgggcgc atgtcgcctc ttatccattg atttttttct aacttttgca
    20341 acaaaggata aatagttcct ggaacgatag tatcaaatcc agcctctcgc aaagtctgaa
    20401 ccaactcata accataccgc tctttttgac caatcatatc caagacacaa ccttcaagaa
    20461 caccttttaa tagctgagtt tctttcatca cttctccctt ctaatctatt ttgtaatacc
    20521 tactagtgac ttcacctata gtatatcact tctacactag tttgtaaagc ataatagtta
    20581 atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ctaccgtatg tatggttact
    20641 gacttcgtca gtttcatcta caacctcaaa aacatgtttt gagctgactt cgtcagtttc
    20701 atctacaacc tcaaaacagt gttttgagct gacttcgtca gtttcatcta caacctcaaa
    20761 aaca
//
LOCUS       DBDESG010000044        20335 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.044, whole genome shotgun sequence.
ACCESSION   DBDESG010000044 DBDESG010000000
VERSION     DBDESG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.044"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..508
                     /locus_tag="ACKB38_001514"
     CDS             <1..508
                     /locus_tag="ACKB38_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGL7980265.1"
                     /translation="RKSNKETSKVRPYFIGGHPGFNCPLLDDEVYEDYYLEFEKEEIC
                     FVPRPFPETGMLDFQDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDK
                     GLKVHFAEFPNLIIWSTLNKGPFITFEPWSGLSTSLEEGDHLEDKKNVCLLEANQVEE
                     LGFEIEVL"
     gene            665..1090
                     /gene="lacA"
                     /locus_tag="ACKB38_001515"
     CDS             665..1090
                     /gene="lacA"
                     /locus_tag="ACKB38_001515"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGL7980266.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFIVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1121..1636
                     /gene="lacB"
                     /locus_tag="ACKB38_001516"
     CDS             1121..1636
                     /gene="lacB"
                     /locus_tag="ACKB38_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGL7980267.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1647..2576
                     /locus_tag="ACKB38_001517"
     CDS             1647..2576
                     /locus_tag="ACKB38_001517"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGL7980268.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKHVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2578..3558
                     /gene="lacD"
                     /locus_tag="ACKB38_001518"
     CDS             2578..3558
                     /gene="lacD"
                     /locus_tag="ACKB38_001518"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGL7980269.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMVKHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQRTATSWKERV"
     gene            3891..4433
                     /locus_tag="ACKB38_001519"
     CDS             3891..4433
                     /locus_tag="ACKB38_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980270.1"
                     /translation="MVSGSVFADSALTTVDKANDIVLNVDGNKFYNVSVSEDIVNAGQ
                     ILEDYFYVDKFGNINLKGTPEELAKNIGISVQEASLMYGAVKELPNVYERGPVGFRFN
                     LGPQVRGMGGWAAGAFATGYAGWHLKQFAVNPVTSGFVAVISGAIGWAVKTAVENYWT
                     VAVATVEVPFVNLVYTIDLP"
     gene            4831..5667
                     /locus_tag="ACKB38_001520"
     CDS             4831..5667
                     /locus_tag="ACKB38_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGL7980271.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5703..6020
                     /locus_tag="ACKB38_001521"
     CDS             5703..6020
                     /locus_tag="ACKB38_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGL7980272.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVQ"
     gene            6020..7711
                     /locus_tag="ACKB38_001522"
     CDS             6020..7711
                     /locus_tag="ACKB38_001522"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGL7980273.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            7920..9326
                     /gene="lacG"
                     /locus_tag="ACKB38_001523"
     CDS             7920..9326
                     /gene="lacG"
                     /locus_tag="ACKB38_001523"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGL7980274.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9449..9628
                     /locus_tag="ACKB38_001524"
     CDS             9449..9628
                     /locus_tag="ACKB38_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGL7980275.1"
                     /translation="MTSEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEW
                     DTISFDSDFMPFQQS"
     gene            complement(9907..10668)
                     /locus_tag="ACKB38_001525"
     CDS             complement(9907..10668)
                     /locus_tag="ACKB38_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGL7980276.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(10956..11918)
                     /gene="nrdF"
                     /locus_tag="ACKB38_001526"
     CDS             complement(10956..11918)
                     /gene="nrdF"
                     /locus_tag="ACKB38_001526"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGL7980277.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLH"
     gene            complement(12106..14265)
                     /gene="nrdE"
                     /locus_tag="ACKB38_001527"
     CDS             complement(12106..14265)
                     /gene="nrdE"
                     /locus_tag="ACKB38_001527"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGL7980278.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(14346..14564)
                     /gene="nrdH"
                     /locus_tag="ACKB38_001528"
     CDS             complement(14346..14564)
                     /gene="nrdH"
                     /locus_tag="ACKB38_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGL7980279.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            14915..15178
                     /locus_tag="ACKB38_001529"
     CDS             14915..15178
                     /locus_tag="ACKB38_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGL7980280.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            15184..16917
                     /gene="ptsP"
                     /locus_tag="ACKB38_001530"
     CDS             15184..16917
                     /gene="ptsP"
                     /locus_tag="ACKB38_001530"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGL7980281.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            17171..19621
                     /gene="phtA"
                     /locus_tag="ACKB38_001531"
     CDS             17171..19621
                     /gene="phtA"
                     /locus_tag="ACKB38_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGL7980282.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            19779..>20335
                     /locus_tag="ACKB38_001532"
     CDS             19779..>20335
                     /locus_tag="ACKB38_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGL7980283.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDG"
BASE COUNT         6455 a         3599 c         4208 g         6073 t
ORIGIN      
        1 caggaagtct aataaagaaa ctagcaaggt caggccttac tttattggtg gacatccagg
       61 atttaattgt cctctacttg atgatgaagt ctatgaagat tattatctag agtttgagaa
      121 agaagagatt tgctttgttc cacgtccttt cccagaaaca ggtatgttgg atttccaaga
      181 tagaagtcca tggctagagg gacaaaaaga aatagatctt agttatgatc ttttcagcac
      241 agatgcagtg actttggatg aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca
      301 tgataaggga ttgaaagtgc attttgcaga atttccaaac ctcatcatct ggtcaacttt
      361 gaacaaggga cctttcatta cctttgaacc atggtctggc ttgtcaacat cccttgaaga
      421 aggagatcat ttagaagata agaagaatgt ttgtctctta gaagccaatc aggttgaaga
      481 attagggttt gagatagaag ttttataaaa aagtaaaaaa taaataacaa aaacaaacaa
      541 aagctgttga cttcttcaaa caatagtagt atattaggtt tgtgaggact aattgactgt
      601 tttttaaaga cagtcaatac actgctggaa tccagcatag aaaaaataaa aaggagtata
      661 caatatgtct attgttatcg gtgcagatgc tgcaggtttg agattgaaag aagttgtgaa
      721 agacttctta gaaaaagaaa acttccacct tgtggatgtt acagctgaag gtcaagactt
      781 tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa gaagaacaaa accttggtat
      841 cgtgattgat gcttatggag ctggtccatt tatagttgca actaagatca aaggaatggt
      901 tgctgcagaa gtatctgacg aacgttcagc ttatatgact cgtggccaca acaactcacg
      961 tatgatcact atgggagcac aacttgttgg tgatgaattg gctaaaaata tcgctaaagg
     1021 atttgttaat ggtaaatacg acggtggtcg tcaccaaatc cgggttgaca tgttgaacaa
     1081 aatgggctaa ttagattata gtaagaaagg taagttaaaa atgagaattg caattggatg
     1141 tgaccacatc gtaactgatg aaaaaatggc ggtttcagaa tttttgaaat caaaaggata
     1201 tgaagtcatt gactttggta cctatgacca tacacggact cactacccaa tctttggtaa
     1261 aaaagtaggg gaagctgtaa ctagcggtca agctgatctt ggagtatgta tctgtggtac
     1321 tggtgttggt atcaacaacg ctgtaaataa agttccaggt gttcgttctg ccttggttcg
     1381 tgatatgaca acagcccttt atgctaaaga acaattgaac gctaacgtta ttggttttgg
     1441 tggtaaaatt actggtgaat tgcttatgtg tgatatcatc gaagctttca tccatgctga
     1501 atacaaacca actgaagaaa acaaaaaatt gattgcgaaa attgaacatg ttgaaagtca
     1561 caatgctcaa caaacagacg caaacttctt tacagaattc cttgagaaat gggatcgtgg
     1621 agaataccac gactaagagg tgacctatga ttttaacagt cacaatgaac ccatccatcg
     1681 atatttccta tcccttggat gagttgaaga ttgatactgt caatcgtgtg gtggatgtaa
     1741 ccaaaacggc tggtggtaag ggactcaatg ttacccgagt actttcagaa tttggcgatt
     1801 ctgttcttgc tactggttta gtgggtggca aacttggtga gtttttggtt gaacatatcg
     1861 ataatcaagt aaagaaagat ttcttctcaa ttaagggaga aactcgtaac tgtatcgcta
     1921 ttctccacgg agacaaccaa acagaagttc ttgaaaaagg tcctgaagta ttggaacagg
     1981 aaggtcaaga gtttttggaa catttcaaaa aactcttgga gtcagttgaa gtagtagcca
     2041 tctcaggtag tctgccagct ggccttccag ttgattacta tgcgagcttg gtagaacttg
     2101 ctaatcaagc tggcaagcat gtagtcttgg actgctcagg tgcagcactt caggctgttc
     2161 ttgaatcacc ccataaacca acagtcatca aaccaaataa tgaagaattg tctcagcttc
     2221 ttggaagaga agtttctgag gatttggatg aattaaaaga agtacttcaa gaacctttgt
     2281 ttgcagggat tgaatggatt atcgtttcac ttggtgccaa cggtactttt gccaaacatg
     2341 gtgacacttt ctacaaggta gatattccta gaattcaggt ggtaaatcct gttggatctg
     2401 gagactctac tgtggcagga atttcttcag gacttcttca caaagaatcg gatgcagaat
     2461 tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca agaaaaaatg actggtcatg
     2521 tcaacatggc caactatcaa gctctatatg atcaattaat agtaaaagag gtataaaatg
     2581 gctttaacag aacaaaaacg tgtacgctta gaaaaacttt ctgatgaaaa tggtatcatc
     2641 tcagctcttg catttgacca acgtggtgct ttgaaacgcc tcatggttaa acaccaaaca
     2701 gaagaaccaa ctgtggccca aatggaagaa cttaaagtct tggtagcaga tgaattgact
     2761 aaatatgctt catctatgct tcttgaccct gagtatggac ttccagcaac taaagctctt
     2821 gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag gttatgacac aacaagcaca
     2881 aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac gtattaaaga agaaggtgca
     2941 gatgcagtta aattcttgct ttactatgat gtagatagct cagacgaact caatcaagaa
     3001 aaacaagcct acatcgaacg catcggttct gagtgtgtgg ctgaagatat cccattcttc
     3061 cttgaaatcc ttgcttacga tgaaaaaatt gcggatgcag gttctgtaga atacgctaaa
     3121 gtaaaaccac acaaagttat cggcgctatg aaagtctttt cagacccacg ctttaacatt
     3181 gatgttttga aagttgaagt tcctgttaac attaaatatg ttgaaggctt cgctgaaggt
     3241 gaagtagttt atacacgtga agaagcagca gccttcttca aagcgcaaga tgaagcaacg
     3301 aacttgccat acatctactt gagtgctggt gtatcagcta aactcttcca agatactctt
     3361 gtatttgctc atgaatcagg tgcgaacttt aacggagttc tttgtggccg tgctacatgg
     3421 gcaggatcag ttgaagctta catcaaagat ggtgaagcag cagctcgcga atggcttcgc
     3481 acaactggat ttgaaaacat tgacgaactc aacaaagttc ttcaaagaac agcaacttca
     3541 tggaaagaac gcgtgtaaga aagtcctcct agtttaggaa catgaatcta aaaaaattta
     3601 aaaaaagttg tatgtaaagg cttacaaaat aacttacttg tgctatactt aaatcacaag
     3661 ttaatatgaa ttagaaagta actatatgaa gtataataaa aataggatat agtttatttt
     3721 acgagctagg aaggaaaaat acggaaacaa tattgccaga ataaactata tttagatgca
     3781 catttcattc attgttttat aaaaggagaa gataaacggc tactaaaaag agttttaaag
     3841 cgttagttgt aggactaggt attgtttcaa tattcttatc agccttacct atggttagtg
     3901 gttctgtatt tgcagatagt gccctaacta cagtagataa agcaaatgat attgttttga
     3961 atgttgatgg gaataaattt tataatgttt cggtttcaga agatattgta aatgctggtc
     4021 aaattttgga agattatttt tatgtagata aatttggaaa tataaattta aaaggcactc
     4081 ctgaagagtt agcaaaaaat attggtattt ctgtacaaga agcaagtttg atgtatggag
     4141 ctgtaaaaga gttacccaac gtttacgaaa gaggtcctgt aggttttcgt ttcaatcttg
     4201 gtcctcaagt gagggggatg ggtggctggg ctgctggagc tttcgctact ggatatgctg
     4261 gatggcattt gaaacaattt gcggttaatc ctgttacatc tggatttgtt gctgtaataa
     4321 gtggtgcgat tggctgggct gtaaaaactg ctgtagaaaa ttattggaca gttgctgtag
     4381 ctacagtaga agtgccgttt gtgaaccttg tttacaccat agatttacct tagaggttat
     4441 ttctttatga atcattcttt taaaaaaata actgtatttt gttttatagt ttcttgtgtt
     4501 ctttgtttat tagacttaat gaattttaaa aatgtagcta cttttttatt tttctgtctt
     4561 cctgtttttg ttttgattta caaaaataaa taaaaacaga gcctctgttt gatgaatttt
     4621 agaacatagt taagttttaa aaaaagttgt atgtaaaggt ttacaaaata acttacttgt
     4681 gctatactta aatcacaagt taatacaagg tgagtgttac taagtaatat taggcatgat
     4741 cacaggtgaa ttagaaatca gctgattttc tagttcattt gtggtcattt tttgtactta
     4801 tataccttta agatataaaa ggaggttgac atgtatcgaa ttctaaatcc aatgaatcac
     4861 aatgtctcgc ttgtcagaaa tgataaggga gaagaggtga ttgtaattgg taagggaatt
     4921 gcattcggaa agaagaaggg ggatttgatt gctgaaaatc aggttgagaa aatctttcgg
     4981 atgaagaccg aagagtccag agaaaacttt atggctcttc tcaaagatgt tccgcttgat
     5041 tttatcacag tgacctatga aatcattgat aagctatcaa agaaatatca ttatccgatt
     5101 caagagtatc tctatgtaac cttgacagat catatttact gttcttatca agctctaact
     5161 caaggaaggt acaaggatag taatctgcca gatatttccg ctaagtatcc tgtcgctttt
     5221 caaatcgcaa atgaagcttt tgaaatttac cgtcagaagc tagcagatca ttttcctgag
     5281 gacgaaatta ttcggattgc ttatcatttc attaatgctg aaggtgaaaa tgaagtggaa
     5341 cttgtggagt cgattgataa gaggaaagaa attctcagga atgttgaaga agttttaacg
     5401 gactatgcaa ttcaacgaac taaaaagaat aaccatttct atgatcgctt tatgatccat
     5461 ttgaattatt tcttggatta tttagacaga tctagagatg ataaccaatc acttctggat
     5521 atggaagatc atattaaaca atcctatcca aaagccttcg agattggttc caagatctat
     5581 gatgtgatta cgcaacatac gggtcttgat ttgtataaaa gtgaacgagt ttatctagtt
     5641 ctacatatcc aacgtttatt gtcataaaaa tttatttaaa actatataag gagaattcta
     5701 tcatgaatag agaagaagta acattgttag gttttgaaat cgtagcctat gctggcgatg
     5761 ctcgttcaaa actattggaa gccttgaagg ctgctgaagc tggtgatttt gaaaaagcgg
     5821 acgctctggt agaggaagct ggtagctgta ttgcagaggc tcaccacgcg caaacaagtc
     5881 tattgactaa ggaagcttca ggtgaggact tggcttatag tgtaaccatg atgcatggcc
     5941 aagaccactt aatgacaact atcttgttaa aagatttgat gcatcattta attgaactct
     6001 acaagagagg agttcaataa tgaataaact aattgcattt atcgagaaag gaaagccttt
     6061 ctttgaaaaa ctatctcgta atatctatct tcgtgctatt cgtgatggtt tcattgcagg
     6121 tatgcctgtt attctcttct caagtatctt tatcttgatt gcctttgtac caaactcatg
     6181 gggctttaaa tggtctgatg aagttgtagc ctttctgatg aaaccttata gctattctat
     6241 gggtattctg gctctcttgg tagctggtac aacagctaag tcattgactg actcagtaaa
     6301 ccggagcatg gaaaaaacca atcaaatcaa ctatatgtca acattgttgg cagcaattgt
     6361 tggtttgttg atgttggcag ctgatcctat cgaaagtggt ctagctactg gattcttggg
     6421 gacaaaaggt ttgctttcag ccttccttgc tgcctttgtt actgtagcca tctataaggt
     6481 ttgtgttaag aacaacgtca ctattcgtat gcctgacgaa gttccaccaa atatctcaca
     6541 agtctttaaa gatgtgattc cattcactct atctgttgtt tctctttatg ctcttgactt
     6601 attagcacgt tattttgttg gttctagtgt ggcagaatca atcggtaaat tcttcgcacc
     6661 actcttctca gcagcagacg gataccttgg tattaccatt atctttggtg cctttgcctt
     6721 cttctggttt gttgggattc atggtccatc tatcgttgaa ccagctatcg cagctattac
     6781 ctatgccaat gccgaagtta acttgaacct tctccaacaa gggatgcatg cagacaagat
     6841 tcttacttct ggtacacaaa tgtttatcgt taccatgggt ggtacaggtg cgacattggt
     6901 cgttccattt atgttcatgt ggttgacaaa atcgaaacgt aaccgtgcaa tcggacgtgc
     6961 ttcagtagtt cctaccttct tcggtgtaaa tgaaccaatc ttgtttggtg cacctcttgt
     7021 tttgaatcca atcttcttca ttccatttat ctttgctcca attgcaaacg tatggatttt
     7081 caaattcttt attgaaactc ttggaatgaa ctcattcact gctaatctac catggacaac
     7141 tccagctcca ctaggtctag ttcttggaac taacttccaa gtgctatcat tcattcttgc
     7201 tgcccttcta atcgtggttg acgttgtcat ttactatcca ttccttaagg tctatgatga
     7261 acaaattctt gaagaagaac gttcaggtaa gtctaatgat gaattgaaag aaaaagttgc
     7321 tgcaaacttc aacactgcaa aagcggatgc tattcttgaa aaagcgggtg tcgatgcagc
     7381 acaaaatacc atcactgaag aaacaaatgt cctcgttctc tgtgcaggtg gaggaacaag
     7441 tggtctcctt gcaaatgctt tgaataaggc agcagcagaa tacaatgtcc ctgtgaaagc
     7501 agcagcaggc ggctatggtg ctcaccgtga aatgttacca gagtttgatc ttgttatcct
     7561 tgcccctcaa gttgcttcaa actttgaaga tatgaaagca gaaacagata agctcggtat
     7621 taaactagcg aaaacagaag gcgctcaata catcaaatta actcgtgatg gaaaaggtgc
     7681 tcttgcattc gtacaagcgc aattcgatta aggctagaga ctctgaaata gtctcccatc
     7741 gttacggaaa tcgctatggc gaatttccta ttattaattc gtcggtaaaa agatatcgtt
     7801 tttacctcct catgtcacaa ttcggtgact tggtacaaga agtgagatgg agaaggatgg
     7861 ctcactgact cctctcctct cacttttact ttatttaaat caagaaatag gtgaaaaaaa
     7921 tgacaaaaac acttccaaaa gactttattt ttggtggcgc aacagctgct tatcaagcag
     7981 aaggtgctac acatactgat ggaaaaggac cagttgcttg ggataaatat cttgaggata
     8041 actactggta cactgccgaa ccagctagtg atttttacaa tcgatatcca gttgacctca
     8101 agctagcaga agagtatggt gtcaatggta ttcgaatttc tattgcttgg tcacgtattt
     8161 tcccgactgg ttacggccaa gtaaatgcta aaggtgttga gttttatcat aatttatttg
     8221 cagagtgtca caaacgtcat gttgagcctt ttgtaactct tcatcacttt gacacgccag
     8281 aagctctcca ctcaaatgga gacttcttaa accgtgaaaa tatcgaacat tttgtagact
     8341 acgctgcctt ctgttttgaa gaatttccag aagtaaacta ttggacaacc tttaatgaaa
     8401 ttggaccaat cggtgatggt caatatttgg ttgggaaatt ccctccaggt atccagtacg
     8461 accttgccaa agtctttcaa tcacaccaca atatgatggt gtctcatgca cgcgcggtaa
     8521 aattgtacaa agagaaaggc tataaagggg aaattggtgt tgttcacgcc ctgccaacta
     8581 aatatcctct agatcctgaa aatccagcag atgttcgtgc agctgagttg gaagatatca
     8641 tccacaataa attcatctta gacgcaactt atctaggtcg ctattcagct gaaaccatgg
     8701 aaggtgtcaa ccatatctta ttagtcaatg gtggtagttt ggatcttcgt gaagaagatt
     8761 ttacagcatt agaagctgca aaagacttga atgatttcct aggaatcaac tactatatga
     8821 gtgactggat ggaagccttt gatggagaaa ctgaaattat ccataatggt aaaggtgaaa
     8881 aaggaagctc taagtatcaa atcaaaggtg ttggtcgtcg tgtagctcct gactatgtac
     8941 cacgcacgga ttgggattgg attatctacc ctcaaggttt gtatgaccaa atcatgcgtg
     9001 tgaagaaaga ttatcctaac tacaagaaga tttacatcac tgaaaatggt ctcggctata
     9061 aagatgagtt cgttgataac actgtttacg atgatggtcg tattgattac gtgaagcaac
     9121 acttggaggt tttatctgat gcgattgcag atggagctaa tgtaaaaggt tacttcattt
     9181 ggtcattaat ggatgtcttc tcatggtcaa acggttatga gaaacgttat ggtctcttct
     9241 acgtagattt tgaaactcaa gaacgttatc ctaagaaatc agctcactgg tacaagaaag
     9301 tagcggaaac tcagattata gactagtaga attagtcatt agatatagaa ttttagtgag
     9361 tcaaaaagat gttcaaagat tttatccaat ctatttatga aaaaaagttt atattataaa
     9421 tttcgaaaaa tgctctcaaa taccgtgttt gacgagtgaa gaattgaaaa gtcttggaaa
     9481 atggtatgtc tcgactggta aagaatggat ttgtcattca gatgatgagc tggaagaatt
     9541 taaaaatcta tttttaaatt ttatcaatcc tgaagaatgg gatactatct cctttgattc
     9601 agattttatg ccgtttcaac aatcgtaacc aatttctcaa aaaagttaaa tcttatattt
     9661 agtactctgt aaaactctta tctaatcacg ttgcttatac tcaatgaaaa tcaaagagca
     9721 actttaaact aggaagcgag tcgcagattt ctcaatgcat agctttgagg aattgggcaa
     9781 aaagtctttg atatagaaaa acgcatagta tcaggtgttt caacacctga tactatgcgt
     9841 tttattgtgg gaagatttac tttttttctt ctgaaattga gttgttaccc aggctctttc
     9901 agtttattaa ggcttgatga ctttaatgtg tttagatagc ttaaaaagga ttgaatcact
     9961 tagtttagaa tctgaaacaa tagtatcaag atttgataca ttataaaaag tataaaaatc
    10021 aaacttattg aacttgctat gatctgcgag taaatatttt ttattagaat tatttaaagc
    10081 gatgcgttga gcctctccct cttcctcgct aaaagtagct agagctccgt tttgaatacc
    10141 attacagcta acgaaagctt tagaaaattg gagattagag agattttgta gggtcaatgt
    10201 accaacaaaa gcacctgtaa tatcgcgata atttccacct attaaaatca aatctgttaa
    10261 ttttcgttcg cttaaaatca gaaaaacagg tagactgttg gttacgacgc ggatattgtc
    10321 aataggcaac tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga aaatagtttc
    10381 tctttcttct actagactgc ctgcaaaatg ggctatttct tgtttttctg ccgtttggag
    10441 ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg gagttggttc gaagtttttc
    10501 agctccacca tgcacacgaa tcagcaaatc tttatcagct aattcctgta aatagcgcct
    10561 tgcagtcata tctgaaacgg ctatttcgtc cataatctgt ttaactgtta tggttccttt
    10621 actatttact atctctaaaa ttttggttaa tttttcttgt ttgagcatat tatcacctcg
    10681 tttcctacta ctatcttacc ataaactagc tcatcattca aatacaaaaa caacaaaata
    10741 aaacaaaaac aaaaatatcg aggtttattt tcaaaacttt cgatattttt attaagttat
    10801 tattttgttg tttctagttt actttttgat ggttaagagt ggtggagaat tatactcaat
    10861 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt acttgagtac ggcaaggcga
    10921 agctgacgtg gtttgaattt gattttcgaa gagtattagt gcaaaccgta gttgtagtca
    10981 tcatcttgca tggcttcaac ttcgccaaga aggtaaccat ttccgacttg agagaagaag
    11041 tcatggttgg aagttcctgt tgaaataccg ttcataacga ttgggttgac atcttcagct
    11101 gaatctggga aaagtggatc ttgtcccatg ttcatgagag ctttattggc attgtagcga
    11161 aggaaggttt taacctcttc agtccaacca acaccgtcat aaagactctc tgtgtagcct
    11221 tcttcatttt cataaagagt atagagtagg tcgtacatcc attctttgag tttttcttgc
    11281 tcttcttcag gtaattcatt gaaaccaagt tggaatttgt aaccaatgta ggttccgtga
    11341 acagactcgt cacgaataat caatttaatg atttctgcaa cgttggcaag tttgttgtta
    11401 ccgagatagt agaggggagt gaagaaacca gagtagaaga ggaaggtttc gaggaagacg
    11461 ctggcaactt tcttttcaag tgggctgccg tttaggtaga tttcgttgac aatctcagcc
    11521 ttcttttgta ggtaaggatt ggtattggtc cattcgaaaa tttcttcaat ctcagcctta
    11581 gtattcaagg tagaaaagat tgatgagtaa gatttagcgt ggacagattc cataaattgg
    11641 atgttattga agacagcttc ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc
    11701 ccagtttcag attgcatagt gtcaagaagg gttaaaccac caaaaacttt tccgaccaag
    11761 tctttctctt tgttagatag ctttctccag tcatccaagt cgtttgataa gggaatacgt
    11821 gtatcgagcc aaaattgctc cgtcagtttt tcccaagttg atttgtcgat gacatcttcg
    11881 atggcattcc agttaatggc tttgtagtaa gtttccattt aaaatctctt tctgtgttta
    11941 gtattgcgaa ctcacaatta tttctacttt accataattc tataggagta tcgcacaaaa
    12001 agtcggaagc ccgactttta aaatgttaca taaattatgt tatgacatag tagatttgat
    12061 tttatcagtg ctgcttaggg aaaaatagtg tttctatgct agaaactaaa tcacacagct
    12121 ttcacattgg ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat
    12181 agacttgatt cccttgttaa aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg
    12241 tttattttcc ctcttccatt cgtaaaggcc ttttggaatg tcactgcgca tgaagagggt
    12301 gagtgaaagt ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg
    12361 catatccata tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc
    12421 agggtaatag attttaccaa ttttcttctc ttggcgttct tcgatacgtt gcgtaatcgg
    12481 gtggatagaa gcagaaacgt cgttgatata gctgatagaa ccatttggcg ctacagcaag
    12541 gcgattttgg tggtaaagac catcttcttg aaccttgtcg cgaagttcag cccaatcagc
    12601 aacaccaggg ataaagacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa
    12661 ttcaccagtt acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt
    12721 gtggaaggta ataccacgtt cacgtgcaat attgtttgac tctaccaagg tccagtagtt
    12781 cataagcata aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag
    12841 ttgttgggca aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct
    12901 atttccatgg tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag
    12961 ggcacgaacc atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac
    13021 gttggttgaa cccaggttac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc
    13081 gttgatcaag cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat
    13141 ctttccatca acaggatttg cacggttagc cgtatcgatg ttgactacat aaggatagcc
    13201 agactcttgt tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt
    13261 gcgaatattt ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa
    13321 tggcacaccg tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt
    13381 acgagccaat tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg
    13441 tactttttca tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa
    13501 gacgttgagg tagacaacac cagcaccttg acgttgcccc aattggttgg agtaagagaa
    13561 gctgtcttca aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt
    13621 gataggtgca ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg
    13681 tgaaagttga agagctgagt tgatagaacg cccgatagag ttcatatcat ccgtcacttg
    13741 gattaggaaa caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg
    13801 agtagcaggt tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc
    13861 attcccatca gcgaaataaa gggcattgaa gaagacacgg tcttccatat tttcaagata
    13921 gtattcaccg tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa
    13981 tgacttgaat tggaagtttt ggtctttgat aaattgagct aattcttcca agaactctgg
    14041 acggtatttc ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt
    14101 gtctttgatt gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc
    14161 caaggcttcc ttgtctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt
    14221 attaagacgg aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc
    14281 taattacaaa agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac
    14341 taagattatg ctaattgttt cagttttcct ggttggaaac ctgaaaagac ttcagttggt
    14401 gtttggataa caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt
    14461 tgctcatcaa gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc
    14521 attttacatt ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc
    14581 aaaatttaat actatcttag tatatcagaa aataaaattt tgtcaatgat ttgaactaaa
    14641 tatagtggtg ttcttttgtt gtttttatat ataaacacaa tatatagtgt ttgtattgtg
    14701 aaatagcaat gattatccta taaattgttt ttaaagaaac tatatcctgt gttttgttat
    14761 ctaggataga agattttcag caagttatct gaaaggaaat tgaaggaaaa tccagaaaat
    14821 gcttgaaaaa aatcctagaa gatggtataa tactaaattg taagggttat cacatataac
    14881 tcaaaaaaag aaagaacaaa aggagagtca aactatggct tctaaagatt tccacgtagt
    14941 ggcagaaaca ggtattcacg cacgtccagc aacattgttg gtacaaactg ctagcaaatt
    15001 tgcttcagat atcactcttg agtacaaagg taaatcagtt aaccttaaat caattatggg
    15061 tgttatgagt cttggtgttg gccaaggtgc tgacgtaact atctcagctg aaggtgcaga
    15121 tgcagatgac gctatcgctg caatctcaga aacaatggaa aaagaaggat tggcataagg
    15181 gaaatgacag aaatgcttaa aggaatcgca gcatctgacg gtgttgcagt tgcaaaagca
    15241 tatctactcg ttcagccgga tttgtcattt gagactatta cagtcgaaga tacaaacgca
    15301 gaagaagctc gccttgatgc cgctctacag gcatcacaag acgagctttc tgttattcgc
    15361 gagaaagcag taggtacgct cggtgaagaa gcagctcaag tttttgatgc tcacttaatg
    15421 gttcttgctg acccagaaat gatcagccaa atcaaggaaa ctatccgtgc gaagaaagtg
    15481 aatgcagaag caggtctgaa agaagttaca gatatgttta tcactatctt tgaaggcatg
    15541 gaagacaacc catacatgca agaacgcgca gcggatatcc gcgacgtgac aaaacgtgta
    15601 ttggcaaacc ttcttggtaa aaaattgcca aacccagctt ctatcaatga agaagtgatt
    15661 gtgattgcgc atgacttgac tccttcagat acagctcaat tggacaaaaa ctttgtaaaa
    15721 gcttttgtaa ccaacattgg tggacgtaca agccactcag ctatcatggc acgtacactt
    15781 gaaattgctg ctgtattagg tacaaataac atcactgaaa tcgttaaaga cggtgacatc
    15841 cttgctgtta acgggatcac tggagaagtg attatcaacc caacagatga acaagcggca
    15901 gaatttaaag cagctggtga agcctatgcg aaacaaaaag ctgaatgggc acttttgaaa
    15961 gatgctcaaa cagtgactgc tgacggtaaa cacttcgagt tggctgctaa tatcggtact
    16021 ccaaaagacg ttgaaggtgt taacaacaac ggtgcagaag ctgttggact ttaccgtaca
    16081 gagttcttgt acatggattc tcaagacttc ccaactgaag atgagcagta tgaagcatac
    16141 aaggctgttc ttgaaggaat gaacggtaaa cctgttgtcg ttcgtacaat ggatatcggt
    16201 ggagataagg aacttcctta cttcgatatg cctcacgaaa tgaacccatt ccttggattc
    16261 cgtgctcttc gtatctctat ctctgagact ggagatgcta tgttccgcac acaaatccgt
    16321 gctcttcttc gtgcgtctgt tcacggtcaa ttgcgtatca tgttcccaat ggttgcgctc
    16381 ttgaaagaat tccgtgcagc gaaagcagtc tttgatgaag aaaaagcaaa ccttcttgct
    16441 gaaggtgttg cagttgcgga taacatccaa gttggtatca tgatcgagat tcctgcagcg
    16501 gctatgcttg cagaccaatt tgctaaagaa gttgacttct tctcaattgg tacaaacgac
    16561 ttgatccaat atacaatggc agcagaccgt atgaacgaac aagtttcata cctttaccaa
    16621 ccatacaacc catcaatcct acgcttgatt aacaatgtga tcaaagcagc tcacgctgaa
    16681 ggtaaatggg ctggtatgtg tggtgagatg gctggtgacc aacaagctgt tccacttctt
    16741 gtcggaatgg gcttggatga gttctctatg tcagcaacat ctgtacttcg tacacgcagc
    16801 ttgatgaaga aactcgacac agctaagatg gaagagtacg caaaccgtgc ccttacagaa
    16861 tgctcaacaa tggaagaagt tcttgaactt caaaaagaat acgttaattt tgattaatcg
    16921 aaaagtccct gcaactcagt tacagggatt tttttgatat tttaaaaaga attttcaaga
    16981 aaatctttct tatagaaagt ccaaccttga aaaagtagtg gtcagaacaa aaaatactta
    17041 aatggttcat aaaattcttg acaagttgga tatttaggag taaactatta accagttaag
    17101 taatagagag gagtttctgc aatttagaaa tgaattgcaa ctagaaatat caaatagaaa
    17161 gagagtttcg atgaaaatta ataagaaata ccttgttggt tctgcggcag ctttgatttt
    17221 aagtgtttgt tcttacgagt tgggactgta tcaagctaga acggttaagg aaaataatcg
    17281 tgtttcctat atagatggaa aacaagcgac gcaaaaaacg gagaatttga ctcctgatga
    17341 ggttagcaag cgtgaaggaa tcaatgctga gcaaatcgtc atcaagataa cagaccaagg
    17401 ctatgtcact tcacatggcg accactatca ttattacaat ggtaaggttc cttatgacgc
    17461 tatcatcagt gaagaattac tcatgaaaga tccaaactat aagctaaaag atgaggatat
    17521 tgttaatgag gtcaagggtg gatatgttat caaggtagat ggaaaatact atgtttacct
    17581 taaggatgct gcccacgcgg ataacgtccg tacaaaagag gaaatcaatc gacaaaaaca
    17641 agagcatagt caacatcgtg aaggtggaac tccaagaaac gatggtgctg ttgccttggc
    17701 acgttcgcaa ggacgctata ctacagatga tggttatatc tttaatgctt ctgatatcat
    17761 agaggatact ggtgatgctt atatcgttcc tcatggagat cattaccatt acattcctaa
    17821 gaatgagtta tcagctagcg agttggctgc tgcagaagcc ttcctatctg gtcgaggaaa
    17881 tctgtcaaat tcaagaacct atcgccgaca aaatagcgat aacacttcaa gaacaaactg
    17941 ggtaccttct gtaagcaatc caggaactac aaatactaac acaagcaaca acagcaacac
    18001 taacagtcaa gcaagtcaaa gtaatgacat tgatagtctc ttgaaacagc tctacaaact
    18061 gcctttgagt caacgacatg tagaatctga tggccttgtc tttgatccag cacaaatcac
    18121 aagtcgaaca gctagaggtg ttgcagtgcc acacggagat cattaccact tcatccctta
    18181 ctctcaaatg tctgaattgg aagaacgaat cgctcgtatt attccccttc gttatcgttc
    18241 aaaccattgg gtaccagatt caaggccaga acaaccaagt ccacaaccga ctccggaacc
    18301 tagtccaggc ccgcaacctg caccaaatct taaaatagac tcaaattctt ctttggttag
    18361 tcagctggta cgaaaagttg gggaaggata tgtattcgaa gaaaagggca tctctcgtta
    18421 tgtctttgcg aaagatttac catctgaaac tgttaaaaat cttgaaagca agttatcaaa
    18481 acaagagagt gtttcacaca ctttaactgc taaaaaagaa aatgttgctc ctcgtgacca
    18541 agaattttat gataaagcat ataatctgtt aactgaggct cataaagcct tgtttgaaaa
    18601 taagggtcgt aattctgatt tccaagcctt agacaaatta ttagaacgct tgaatgatga
    18661 atcgactaat aaagaaaaat tggtagatga tttattggca ttcctagcac caattaccca
    18721 tccagagcga cttggcaaac caaattctca aattgagtat actgaagacg aagttcgtat
    18781 tgctcaatta gctgataagt atacaacgtc agatggttac atttttgatg aacatgatat
    18841 aatcagtgat gaaggagatg catatgtaac gcctcatatg ggccatagtc actggattgg
    18901 aaaagatagc ctttctgata aggaaaaagt tgcagctcaa gcctatacta aagaaaaagg
    18961 tatcctacct ccatctccag acgcagatgt taaagcaaat ccaactggag atagtgcagc
    19021 agctatttac aatcgtgtga aaggggaaaa acgaattcca ctcgttcgac ttccatatat
    19081 ggttgagcat acagttgagg ttaaaaacgg taatttgatt attcctcata aggatcatta
    19141 ccataatatt aaatttgctt ggtttgatga tcacacatac aaagctccaa atggctatac
    19201 cttggaagat ttgtttgcga cgattaagta ctacgtagaa caccctgacg aacgtccaca
    19261 ttctaatgat ggatggggca atgccagtga gcatgtgtta ggcaagaaag accacagtga
    19321 agatccaaat aagaacttca aagcggatga agagccagta gaggaaacac ctgctgagcc
    19381 agaagtccct caagtagaga ctgaaaaagt agaagcccaa ctcaaagaag cagaagtttt
    19441 gcttgcgaaa gtaacggatt ctagtctgaa agccaatgca acagaaactc tagctggttt
    19501 acgaaataat ttgactcttc aaattatgga taacaatagt atcatggcag aagcagaaaa
    19561 attacttgcg ttgttaaaag gaagtaatcc ttcatctgta agtaaggaaa aaataaacta
    19621 atgaaaaatg aaagtctcga taaagaggct ttcattttta ttatgtatat atgtaaaatt
    19681 cttgacaagc aatattaaaa agagtaaact attaactagt taattaaccg gtttattact
    19741 ttatagtgaa tcaaatatac ttaagaaaag aggaaagaat gaaaattaat aaaaaatatc
    19801 tagcaggttc agtggcagtc cttgccctaa gtgtttgttc ctatgagctt ggacgttacc
    19861 aagctggtca ggataagaaa gagtctaatc gagttgctta tatagatggt gatcaggctg
    19921 gtcaaaaggc agaaaacttg acaccagatg aagtcagtaa gagggagggg atcaacgccg
    19981 aacaaattgt tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc
    20041 attactataa tggcaaggtt ccttatgatg ccatcatcag tgaagagctc ctcatgaaag
    20101 atccgaatta tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggttatgtca
    20161 ttaaggtaaa cggtaaatac tatgtttacc ttaaggatgc agctcatgcg gataatattc
    20221 ggacaaaaga agagattaaa cgtcagaagc aggaacgcag tcataatcat aactcaagag
    20281 cagataatgc tgttgctgca gccagagccc aaggacgtta tacaacggat gatgg
//
LOCUS       DBDESG010000045        20203 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.045, whole genome shotgun sequence.
ACCESSION   DBDESG010000045 DBDESG010000000
VERSION     DBDESG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.045"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            96..344
                     /locus_tag="ACKB38_001533"
                     /pseudo
     CDS             96..344
                     /locus_tag="ACKB38_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            358..698
                     /locus_tag="ACKB38_001534"
                     /pseudo
     CDS             358..698
                     /locus_tag="ACKB38_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            691..1071
                     /locus_tag="ACKB38_001535"
     CDS             691..1071
                     /locus_tag="ACKB38_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGL7980284.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            1084..1317
                     /locus_tag="ACKB38_001536"
                     /pseudo
     CDS             1084..1317
                     /locus_tag="ACKB38_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1320..1907
                     /locus_tag="ACKB38_001537"
     CDS             1320..1907
                     /locus_tag="ACKB38_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGL7980285.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            2308..3117
                     /locus_tag="ACKB38_001538"
     CDS             2308..3117
                     /locus_tag="ACKB38_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGL7980286.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            3122..3823
                     /locus_tag="ACKB38_001539"
     CDS             3122..3823
                     /locus_tag="ACKB38_001539"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGL7980287.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            3848..4765
                     /locus_tag="ACKB38_001540"
     CDS             3848..4765
                     /locus_tag="ACKB38_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGL7980288.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            4793..6334
                     /locus_tag="ACKB38_001541"
     CDS             4793..6334
                     /locus_tag="ACKB38_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGL7980289.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            6353..6811
                     /locus_tag="ACKB38_001542"
     CDS             6353..6811
                     /locus_tag="ACKB38_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGL7980290.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            6813..9035
                     /locus_tag="ACKB38_001543"
     CDS             6813..9035
                     /locus_tag="ACKB38_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase domain-containing protein"
                     /protein_id="HGL7980291.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            9075..10184
                     /locus_tag="ACKB38_001544"
     CDS             9075..10184
                     /locus_tag="ACKB38_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGL7980292.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            10341..11231
                     /locus_tag="ACKB38_001545"
     CDS             10341..11231
                     /locus_tag="ACKB38_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGL7980293.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            11256..11579
                     /locus_tag="ACKB38_001546"
     CDS             11256..11579
                     /locus_tag="ACKB38_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980294.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            11569..13560
                     /locus_tag="ACKB38_001547"
     CDS             11569..13560
                     /locus_tag="ACKB38_001547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HGL7980295.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMNKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            13576..14052
                     /locus_tag="ACKB38_001548"
     CDS             13576..14052
                     /locus_tag="ACKB38_001548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HGL7980296.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            14086..14667
                     /locus_tag="ACKB38_001549"
     CDS             14086..14667
                     /locus_tag="ACKB38_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980297.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            14732..15739
                     /locus_tag="ACKB38_001550"
     CDS             14732..15739
                     /locus_tag="ACKB38_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HGL7980298.1"
                     /translation="MDTNLFSKINTTISVKENDFITEEKFQKIIQSKDTETLAFILES
                     TPYHLSIDILEDPSQTEISLMTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYG
                     S"
     gene            15729..16049
                     /locus_tag="ACKB38_001551"
     CDS             15729..16049
                     /locus_tag="ACKB38_001551"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HGL7980299.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            16112..17887
                     /locus_tag="ACKB38_001552"
     CDS             16112..17887
                     /locus_tag="ACKB38_001552"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HGL7980300.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            17888..19273
                     /locus_tag="ACKB38_001553"
     CDS             17888..19273
                     /locus_tag="ACKB38_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HGL7980301.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            19296..19907
                     /locus_tag="ACKB38_001554"
     CDS             19296..19907
                     /locus_tag="ACKB38_001554"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HGL7980302.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            19949..>20203
                     /locus_tag="ACKB38_001555"
     CDS             19949..>20203
                     /locus_tag="ACKB38_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980303.1"
                     /translation="MDKALSLSKLFLLTVSGYLFQNFGLLDNENVSTNLDLGLVNRKC
                     NYIESKRVITRYLSFMELLLYTISKVKLLEDILMPQPIVPV"
BASE COUNT         6819 a         2875 c         3992 g         6517 t
ORIGIN      
        1 aaagcaactc agttagttga tgaaatggaa caagtaggtt taatccgatt tgatgaattt
       61 ggaaatgttg gaattttagt cttggaggga caataatgaa gcgtattacc gctaatcaat
      121 atcagacatc agagcgttat tataagctcc ccaaactttt atttgagagt gaacggtata
      181 agaatatgaa actggaggtt aaggtggttt actcagtttt aaaagatcgg ttggagttgt
      241 ctttgagcaa aggttggatt gatgaggatg gggctattta tttgatttat tccaattcaa
      301 atttgatggc acttttaggc tgttcaaagt caaaattact ctccatgtga gtttgaagtg
      361 acatttttag atgattacca taaaaaacat aactacccac tattttacga atcctatctt
      421 caaaacgtta tggaattcct tgaaagtcaa gacataaaga atggggttga tgcctttgta
      481 gatgatcatc aaaatctcgt ttttgtttta tatggacaag gctatcgagc cgagggaaaa
      541 gagggaatac ttacaaccca agtaactgta aaagcttatg atgaagacaa gaaaccgatt
      601 aacttcgcaa atttattaga ttccttaatt tattagattc cttaatcgtg tcagaatatc
      661 aaatggaacc gaatctttgg gaggtctccc atgattgatc tctatctaag taaaaataga
      721 caaagaaatc aacttctttt agatttcttc caaaactatg gcatagaggt atcttgtcat
      781 tcagtttctg aaatgacaaa ggacaaatta attgagatga tgagctattc ttcagattgt
      841 tttgagtttt tatctcccaa tttattacga tttaagaacc gtgacaatct aagattaacg
      901 gatttcgttg agataatttt aaaaaatcct gaactaacca tcagacttcc tcttgcggtt
      961 tcaaataagc gagtttatcc aagtctgaat ttggaagagg ctagagctct attgccaaga
     1021 gatacgaaac aattgattta catggcgcaa acacattatt tatctaacta aaggagaagc
     1081 aatatggctg aacgattttg ggagaacttg tccattattt tggctgaaag aaatatcagc
     1141 tggattgagt taaccagaaa aatgtttgcg ggagagtttc actatccaag tgaattgaat
     1201 cgtttgtatc aaaagattcg ccactagaag atggaacaat gaatgcctca aagtccatgg
     1261 gtagaaagga ttgtgcaggt attagattta gattatgaag atttatttcg gaggtaacta
     1321 tgaaaagaat aattccagtt tatatattcc aacaagtaaa tgtcctattg gtatctctat
     1381 acttattgaa atttctttgt atcggtgagt taactatact acagattctc tatggttcgt
     1441 cactcatttc ttttttatgg atgtatggcc aacgcaaaca agcgcataag gtcaatatga
     1501 aatctaggat gaaatggctt ggtgttgaat ttgttagcct actgattata agtctatgtt
     1561 ttagtctgat tcatgctcaa ggaagcacga atcaagcaaa cttaattggc cttcaacatc
     1621 aaattccttg gttttcattt ttattgttct taatcaatgc gagtatggtt gaagaatttc
     1681 tgtatcgaga aattttatgg aatttggtca gaaaattaga tatcagagtt gctttgacaa
     1741 gtgttttatt tgccttagca catcatacag gaaccattct agcttggtgt ttgtatgttt
     1801 cacttgggat gtttttaggt atggtgcgct ataaatctga cttatggggc agtatgggac
     1861 tacatttggt gtggaaccta ctagtttata gtttgctgct tttttaaaat aggtgacctg
     1921 atttatctca taaatctgta gtttttatgg gattctcttt taatttagaa ataaaatata
     1981 ttacctatat aaaataaaga aactagaaat attttttcaa tttttttaga aataaaatat
     2041 tatatagaaa ttccgaaaag aaataataaa accaaaataa aatattacta aaatatttta
     2101 atatgaacat attattagtg ataaacgttc ttacaataca tataattacg ataaaaagaa
     2161 taaaaactat tatattttta tgtttgttgt tctgttatga aacgaaagaa attggaaact
     2221 agtttctaat ttatgaaaaa tattgaaaaa aaaaaaaaaa gtgttaaaat aaagaatgta
     2281 aacgtttact tcaactaagg agctcatatg ttactgcaaa aagaactaat tccaatgata
     2341 gaagctaact taccaaatat ggcatatgct gaaaaagaca ttgctaaatt cttcttaaaa
     2401 cagcaacctc tgaatgatta ttcatctaaa gcattgtgcg aataccttaa tgtatccaaa
     2461 gcaacattga ctcgatttgc gaaaaaatgt ggttttaaag gttttagaca attcattttc
     2521 aaataccaag agatgattca tgagaaagaa aagttggcat tatatacaga ggcaacagaa
     2581 aaagttttat ccgactatga ggaaatgttg agaaaaactt acacggttct tgatgaagtt
     2641 caacttgagc gtattgctga gatgatagaa actgctgagc gtgtatatct ctacggtaaa
     2701 ggaagttctg ttcttgcttt acaagaaatg aagatgagat ttatgcgtct cggagtgatt
     2761 ggtgaagtat tatcagacga ggatatgatt ttgtggagta gcttactact taatgaaaat
     2821 tgccttgtca ttggagcatc catttcaggt caaactgata ttgtactaga aggtctacaa
     2881 aaagctgcag ataaaggcgc taaaacagtt ttaatgacta caagaaaatt tgacgaagaa
     2941 gattgtttct ttgatgaact attgttatta gcttcgaccg atcatctctc gtatggcaat
     3001 cgcatatcac ctcagtttcc aatactttta attacagact gcttattctc taattatctg
     3061 gaaagtccag agagacaata ttattacaat caaactatta tccataagga ggaataataa
     3121 aatgagccaa atttggacta aagaaaaatt tataagccaa gttcaaggtg gagtcattgt
     3181 ttcttgtcaa gctttacctg gtgaagccct ttataatgaa gaatttagct tgatgccttt
     3241 tatggctaaa gcagctttag aggcaggagc agtgggcatt cgcgcaaatt ctgtgcgtga
     3301 tattaaagca attcagaaag tagtagattt accaataatt ggaattatca aaagggatta
     3361 tccacctcaa gaaccatata ttactgctac gatgaaagaa gtagatgaac ttgtagaatg
     3421 cggaacaaca gtcattgcat ttgatgcaac tttaagacca agatatgatg gcttagttgt
     3481 cagtgaattt atcaaaaaaa taaaagaaaa atatccgaat caattgctga tggcggatgt
     3541 aagtaattta gatgaaggtc tctatgcatt taaatcaggc gttgattttg ttggtacaac
     3601 attatcaggt tacacaagta caagtgtaca atcagatgag cctgattttg aactaatgaa
     3661 aaaattggct gattttaata ttccggtaat tgccgaagga aaaattcatt atccagaaca
     3721 attaaaaaaa gcttatagtt taggtgttac cagtgtagtc attggtggag cgattacacg
     3781 tccaaaagaa attgctcagc gatttattaa tgtcatcaaa taaaaaacaa tataaaggag
     3841 aaatctgatg aaagatttaa ctaaatacaa aggcgttatc cctgcatttt atgcttgcta
     3901 tgatgaaaat ggtgaaatta gccaagatcg tgtaaaatct ctggtacaat atttcattga
     3961 caaaggtgta aaaggtatct atgtaaatgg ttcttcaggt gaatgtattt accaaagtgt
     4021 agaagatcgt aaacaaatta ttgaagctgt tatggaagtt gctaaaggta aattaacagt
     4081 tatcaaccat attgcatgta ataacacgaa agatagtatc gaattggcaa aacattcaga
     4141 aagtgttgga gtcgatgcta ttgcagctat cccacctatt tatttcaaat tgccagagta
     4201 ttcaatcgca gcatattgga atgcaatgag tgaagctgcg tcaaatacag attttattat
     4261 ctataatatt ccacaattgg caggggttgc gttgactggt agtttgtatg caacaatgcg
     4321 tcaaaatcct cgtgtgattg gagttaaaaa ttcttctatg cctgtacaag atattcaaat
     4381 gtttgtagct gcaggtggag aagattacat tgtattcaat ggtccagatg aacaatatct
     4441 tggtggtcgc ttgatgggag cagaagctgg tattggtggt acttatggcg ttatgccaga
     4501 tttgttcttg aaattggaaa gtttgattca agaacgagat ttagatacag ctaaaaaact
     4561 tcaatatgct atcaatgaag ttatctataa gatgatatca ggtaaggcaa atatgtatgc
     4621 tgtagcaaaa gaagttttgc gtctaaatga aaaacttgat ttaggttctg ttcgtcaacc
     4681 tttagaagca ttggcagaag gtgacttgga agttgcaaaa caagcagcag aacttattca
     4741 acaagcacga aaagaatttt tataataaat aaaatcaata tggagatatg ttatgggcac
     4801 aacaggattt acaataattg acttaattat cttgattgtt tatttacttg cggtgttggt
     4861 tgcaggtatc tatttctcta aaaaagagat gaaaggaaaa gagttcttta aaggagatgg
     4921 ttcggttcct tggtatgtta cttcggtatc catttttgcc acaatgctca gtccgatttc
     4981 cttcttggga ctcgctggta gctcttatgc aggtagctgg attttatggt ttgctcaatt
     5041 agggatggta gtagctattc cactgacaat tcgttttatc ttacctatct ttgcacggat
     5101 agacatcgat acggcatatg attacttgga taaacgtttt aattctaaag cacttcgtat
     5161 tatttcagca ctcttgttta ttatttatca attgggacgt atgtctatca ttatgtacct
     5221 cccatcagct ggtttatcag tattgacagg aattgacatc aatattttga ttattttgat
     5281 gggtgtagtt gcaattgttt attcttatac tggtggtcta aaatccgtat tatggacaga
     5341 ctttattcaa ggtgtgattc tgattagtgg tgtcgtttta gctttatttg tactgattgc
     5401 taatattaaa ggtggctttg gtgcagtagc agaaacatta gcaaacggga aattccttgc
     5461 tgcaaatgaa aaacttttcg atcctaactt gctttcaaac tccatctttt taattgtgat
     5521 gggttcaggc tttacaatct tgtcttccta tgcttcatct caagatttgg ttcaacgttt
     5581 tactacaaca caaaatatta agaaacttaa taagatgttg ttcacaaacg gtgttttgtc
     5641 acttgcaact gcaacagtct tttacttgat tggtacaggc ttgtacgtat tctatcaagt
     5701 acaaaatgca gatagtgcag ctagcaatat ccctcaagac caaatcttta tgtactttat
     5761 tgcataccag ttaccagtag gtatcacagg tttgatcttg gcagcgattt atgcagcatc
     5821 tcaatcaact atttcaacag gtttgaactc tgttgcaact tcatggacat tggatattca
     5881 agatgtcatt tctaaaaata tgtcagacaa tcgtcgtacg aaaattgcac aattcgtatc
     5941 tctagcagta ggtttattct caattggtgt ttccattgtc atggctcact cagatattaa
     6001 atctgcatac gaatggttca atagtttcat gggacttgta cttggtctac ttggtggtgt
     6061 atttattctt ggatttgttt ctaaaaaagc aaataaacaa ggtgcttatg cagcgctgat
     6121 tgtatcaacc atcgtcatgg tatttattaa atacttcctt cctccaacag ctgttagcta
     6181 ctgggcatat tcattgattt caatctctgt atcagtagtt tcaggttata ttgtatctgt
     6241 tcttactgga aataaagtat ctgcacctaa atatacaacg attcatgata ttacagaaat
     6301 taaagcggat tcaagttggg aagttcgtca ctaatagaaa agagaaaata atatgattat
     6361 ttctgaacaa tctgatttta aacgatatgc ttctgtcaat aaatattttt caaaagtctg
     6421 tgatttttta gaaaatacaa atttgacaga tttagttgat ggaaaaattg atattgacgg
     6481 ggaaaatgtt tttgcaaatt gtatgactta tctagctgat ggagttccag gagatatttt
     6541 tgaaactcat aagaaatatt tagatattca tattgttgtt gaaaatactg aaaaaatggc
     6601 agtaacttcc ccagttcgtg cccagtcacg tgtacctttt agcgaagaga aggatattgc
     6661 tttttacgat agcaaagact atcaaattgt tgaattgctt ccagggaata tgttggtgac
     6721 ttttgaagaa gatcttcacc aaccaaagat tcattgcaat gatgaaactg ttaaaaaact
     6781 tgttatcaaa gttttaaacg aggaaaaata aaatgaaaaa aaatattaaa caatatgtaa
     6841 ccttaggtac tgtagtggta ttatcagcat ttgttgctaa ctcagttgca gctcaggaga
     6901 ctgaaacttc tgaagtatca acaccaaagt tggtgcaacc tgttgcacca acgactccga
     6961 tttcggaagt acaacctaca tcggataact cttcggaagt tactgtacaa cctcgaacag
     7021 ttgaaactac tgttaaggat ccatcttcta cagcggaaga aactcctgtc ttagaaaaaa
     7081 ataatgttac tttaacaggg ggcggagaaa atgttactaa agagttaaag gataaattta
     7141 ctagcggtga ctttactgta gtgattaagt acaatcagtc aagtgagaaa ggcttacaag
     7201 ctctgtttgg aatatctaat tccaaacccg gtcaacaaaa tagttatgta gatgtgttcc
     7261 ttagagacaa tggtgagttg gggatggaag cgcgtgatac ttcttccaat aaaaataacc
     7321 tagtatccag acctgcttca gtttggggta agtacaaaca agaggctgtg actaacactg
     7381 ttgcagtagt agcagattca gtcaaaaaaa catattcttt atacgcaaat ggtacaaaag
     7441 tagtagaaaa gaaagtggat aatttcctaa acatcaagga tattaaaggt attgattact
     7501 atatgcttgg gggagtgaaa cgtgcaggaa aaacggcgtt tggttttaac ggaacactag
     7561 aaaatatcaa attctttaat agtgcattgg atgaagaaac tgttaaaaag atgacaacaa
     7621 acgctgttac tggacattta atttatacgg ctaatgatac aacaggttct aactatttcc
     7681 gtattccagt tctgtatact tttagcaatg gtcgggtatt ttcaagcatt gacgctcgtt
     7741 acggtggaac tcatgatttc ttgaataaaa ttaatattgc tacaagttat agtgatgata
     7801 atggtaagac atggactaaa ccaaaattaa cattggcatt cgatgatttt gcgccagtac
     7861 cattagaatg gcctcgtgaa gttggtggac gtgacttaca aatcagcggt ggtgcaacct
     7921 atattgactc tgttattgtt gaaaaaaaga acaaacaagt actcatgttt gctgatgtga
     7981 tgcctgctgg agtaagtttt agagaagcaa ctagaaaaga ttcaggttat aaacaaattg
     8041 atggtaatta ttaccttaaa ttaaggaaac aaggtgatac tgattacaat tatactattc
     8101 gtgagaatgg tactgtatac gacgatcgta ccaacagacc aactgaattt tcagtagata
     8161 aaaatttcgg tattaaacaa aatggtaatt atttgacggt agagcaatat tcggtttcat
     8221 ttgaaaataa taaaaagact gaatatcgta atgggactaa agtccatatg aatatcttct
     8281 acaaagatgc attgtttaaa gtagtgccaa cgaactatat tgcatatatt tctagcaatg
     8341 atcatggcga atcttggtct gcaccaactt tattacctcc tataatggga cttaatcgga
     8401 atgcgccata tttaggtcct ggacgtggaa tcattgaaag ctcaactgga cgtattctta
     8461 ttccgtctta cactggtaaa gagtctgcgt tcatttatag tgacgataat ggagcatctt
     8521 ggaaagttaa agtagtgcca cttccttcta gttggtcagc agaagcacaa tttgtagaat
     8581 tgagtccagg agtaattcaa gcatatatgc gtacaaataa tggtaaaatt gcatatttaa
     8641 caagtaaaga cgcaggtact acttggagtg caccggaata tttgaaattt gtttcaaatc
     8701 caagttatgg aacacaatta tcaatcatca attatagcca attgattgat ggtaaaaagg
     8761 ctgtcatttt aagtactcca aactccacaa atggtcgtaa acacggacaa atttggattg
     8821 gtctaattaa tgatgataat acaattgatt ggcgttatca tcacgacgtt gattatagta
     8881 actatggata ctcatattca acattgacag agttaccaaa tcatgaaatt ggattgatgt
     8941 ttgaaaaatt tgattcatgg tctcgtaatg aacttcatat gaaaaatgtt gtaccatata
     9001 taacatttaa gattgaagat ctgaaaaaga attaaagctg aaatttgaaa atatataaaa
     9061 agaggataaa aattatggta aattacggta ttgttggagc tggatatttt ggagctgatt
     9121 tagctcgctc aatgaacaaa attgaagatg caaaagtggt tgcggtattt gacccaaatc
     9181 atggagaaga agttgctcaa gagttgggat cagatgtttg tgcaagttta gatgaacttg
     9241 tagcacgtga agatattgat tgtgtgatcg tagcttcacc tagctacctt caccgtgaac
     9301 cagttgtgaa agctgctcaa catggcaaac acgtattttg tgaaaagcca attgcattgt
     9361 cttatgaaga ttgtaaagcc atggttgacg catgtaaaga aaataatgtc atctttatgg
     9421 ctggtcacat catgaacttc tttaacggtg tacaccatgc taaagaattg attactcaag
     9481 gtaaaatcgg taaagttctt tattgccatg ctgctcgtac aggttgggaa gaacaacaac
     9541 caactgtatc atggaagaaa cttcgttctc aatctggagg acatttgtac caccatattc
     9601 atgaattaga ttgcattcag tttatcatgg gaggacttcc tgaaaaagcg acaatggtag
     9661 gaggcaatgt atatcataaa ggtgaaaact ttggtgatga agatgatatg ctcattgtaa
     9721 acttagaata ctctgatgat cgttatgctg ttttggaata tggtaatgct ttccgttggg
     9781 gtgaacacta cgtcttgatt caaggaactg aaggagctat caaacttgac ttgttcaata
     9841 ctggcggtac tcttcgtgtt aaaggtgaag gagaatcaca cttcttagtt catgaaactc
     9901 aagaggaaga tgatgatcgt acagctatct ataccggtcg tggtatggat ggagcaattg
     9961 cgtacggtaa accaggagta cgttgcccat tatggttgca aacatgtatt gataaagaaa
    10021 tggaatatct acatgacatc attaaaggtg gagaaattac agaagaattt gaaaaacttc
    10081 tcaatggtgt agctgcttta gaatcaatcg ctaccgctga tgcatgtact ttatcagtta
    10141 aagaagatcg aaaagtaagt ctttcagaaa tcacaaatgc ttaacttttg taaaacagaa
    10201 tagtaaattc ttgtcattat ataatttcta aagttctgtg atacaactca ttgaataaag
    10261 aaatagagat gggactggga taatgcccag tcccattttt tatcaaaaag taatgagatc
    10321 aaaaatgtgg gagtgttgaa atgaagatta taggtatcga tattggcgga acaacaatta
    10381 aggcagattt atacgatgag tttggaacga gtttgaatca tttcaaagag atagaaacaa
    10441 ttattgacta tgatttggga acgaatcaga tattaaatca ggtctgtgat ttaattggtg
    10501 agtatacttt aaatcattca attgatggtg ttgggatttc cactgctgga gttgttaatg
    10561 ctaatactgg agaaatcatc tatgcaggct atacaatacc agggtatatc ggagtaaact
    10621 ttactgccga aatagaaaaa cgttttgggt tgtatacttt tgttgaaaat gatgttaatt
    10681 gtgctgcatt aggtgaattg tggaagggac aagccaaaga taagaaaaat gtagtaatgg
    10741 ttactattgg aacaggtata ggaggcagta ttattgtcaa cggacaaatt gttaacggat
    10801 ttaactatac tgctggtgaa gtaggttata ttcctgtagg taattcggat tggcaaagta
    10861 aagcctcaac aaccgcattg attcatttat atcaaaaaaa gagcttgaaa actaatcaaa
    10921 ctggacgtac tttcttcact gatttaagat ctggagataa agttgctgaa gaaacttttg
    10981 aaatttttgt agaaaatcta acaaaaggtt tattaacgat ttcttatcta cttaatccag
    11041 aaattctcat attaggaggt gggattctgg atagtaagga tattttgtta cctgaaattc
    11101 aaagttcttt agctaaaaat gcaatggata ataggttttt acctaaaaat cttgtggcag
    11161 ctacattagg aaatgaagct ggtcgtatag gagctgtaaa aaatttctta gatagaattt
    11221 ctaataaata gtatgtaaga taaggaggtg tcacaatgac taactctgta ttttcgacaa
    11281 tgcaagatat tgagaatgtt gcaaccgata ttataaaatc atatgataat gagatttata
    11341 cttataaagc tgtttcccaa gaagaattgg aaaaactaga aaaaagttat gatgaaaaaa
    11401 gtcacgaaga attagtttca atagaaagca atttagaaat gaaacaacag aaccttattg
    11461 atgaggttaa taaaacaatc aaggaaaatg atgcaaatat tcagtatatt tcatcaagta
    11521 ggagaggaga atttgtagaa aaaattattg gtagggtggt agaaaaatat ggccattagt
    11581 cagatgaaaa gaatctctct actattttct aaaagtagtc ttgatgatgt tttaaaaact
    11641 attcaagaac tagagtcagt gcagttccgt gatttaaagg ttcaggataa ctggtcagaa
    11701 gctctagaaa aagatgaagt tgtatttcca actattcaaa tttttcatac ttctaattcc
    11761 aatcatgggg ttattgaggg aaatgatgcc ttgacttatt tgatgaatca acaacaacat
    11821 ttagaagcaa ctgtagagaa attacaagaa tacctaccga aagaaaacac gtttaaatta
    11881 ttgcagcaac ctccgataac tacctcttat gaagaattag agaaatttgg taaagctaat
    11941 gttgctgagg gtgttcttaa aaaagtgaat catcaaatta acagagttca tgaattagaa
    12001 agacacattc aaagtaataa tgaggaaata gagcgattaa taaagtggga aaaattagaa
    12061 attgttcctg cgaatttaga acaattttct ttctgtaaag gaaaagtcgg aacaattcca
    12121 aggactgaag ataatcgctt atacaatagt cttttagaaa acaatattga agttcaagaa
    12181 atattttcta atgatagaga gtacggtgtt gttgttttct atcagtctag ttactctata
    12241 gattttgatg aatacttatt tgaaccattt gattattcta gaaaggaatt accgaagcag
    12301 cgagtagtag atttagatca agaaaacatg cagttaataa ctgaaaaaga gaatattatc
    12361 gcatcgttgc aagattcaaa gaaatatttg atagatttac aatggcaaat agactatatt
    12421 ttatctatct atgctcgtca aatctctaag aataactttt tgtgcactcc gcatctagtt
    12481 gcattagaag gatggataga agaaactcgt attttatatt ttataaaagt tatggatgag
    12541 cattttggac attctattta tatttatgaa tcggaaacat tgacggataa tcaagatgaa
    12601 atacctatca aattaacgaa tcattcttta attgaaccat ttgaattatt gacagaaatg
    12661 tatgctctgc ccaaatatta tgagaaagat cctacacctg tattagcacc attttacttt
    12721 acattttttg gaatgatggt tgctgattta ggctatggtt tactattgtt tttaggaaca
    12781 atgttagcat taaaaatttt tcatctacct tcagcaacta agagattttt aaaattcttt
    12841 aatatattag gggtagccgt tgcaatttgg ggtggaatct atggctcatt ttttggatat
    12901 gagttgccat ttcatctgat atctacaacc tctgatgtca tgactatatt agtagtgtca
    12961 gttgtgtttg ggtttattac agtatttgca ggtttgttag cttcaggact acaaaaagta
    13021 agaatgaata aatatgcaga agcatataat tcaggatttg cgtggtgtgt tattctgctt
    13081 ggcttgttat ttattgctgt tggaatgttg atgcctgata tgagaccgtt atttgtatta
    13141 gggaaatggg tatctatttt taatgctgtg gggattttga ttgtttctat tattcaaacc
    13201 aaaagcttgt caggtattgg agcaggattg tttaatctat ataacatttc atcttatata
    13261 ggtgatttag ttagtttcac tcgattgatg gcattaggat tatctggagc aagtatagca
    13321 tcagctttca atttaattgt tggtttgttt ccgggaatat tggctaaact gacaattgga
    13381 ttagtattat tcattctttt acatgcgatc aatatttttc tatcgttact atcaggatat
    13441 gttcatggag cacgtctgat atttgttgaa ttttttggta agttttatga gggtggagga
    13501 aaaccatttc aacctttgaa ggcttctgag aaatatatta aggttattac aaagaattaa
    13561 tggaggatat atataatgga acatttagca acttattttt caacctatgg aggagctttc
    13621 ttcgctgcat tgggaattgt attggcggtt ggattaagcg gtatggggtc tgcttatgga
    13681 gttggtaagg ctgggcaatc tgccgcagct ttactgaaag aacagcctga aaagtttgcc
    13741 tcagctttga tattgcaatt attgcccgga acacaaggat tatatggttt tgttattgga
    13801 attttaattt ggttgcaatt aactccagaa cttcctttag aaaaaggcgt tgcttatttc
    13861 tttgtagctc ttccaattgc tattgtagga tacttttcag ctaagcatca aggaaatgta
    13921 gcagtagcgg gaatgcaaat cttggctaaa agaccaaaag aattcatgaa gggagcaatt
    13981 ttagctgcca tggtagaaac ctatgcaatt cttgcttttg tcgtatcatt cattttgacc
    14041 cttcgtgtat aagaaataaa tttgcaattc aaaggaggtg tctaaatgag caatttagaa
    14101 aacttacgag agtctgttat tgaacaagct catgaaaaag ggcgtatgaa attattggat
    14161 tccaaaaaga agattgatga tgaatttgaa atgcaaaagt cgctcattat aaagaaaaaa
    14221 gaagctgaac atgaacgaaa gttaaaagaa ttgcaacaga aatatcaaat aatttttcaa
    14281 caattaaaaa ataaggaacg ccaatcaacg ttagtatcaa aacagaaaat attaaaagaa
    14341 ctttttcaat ctgctttact agaaatggaa tcttggagtg cagataaaga aatggagttc
    14401 atctatcgaa ttctggaacg atattcacaa caagaggtca tagtaacctt tggggaacgg
    14461 actttagcta aattcaattt ggaacaatta gagaaattga aattctcttt tccaaattat
    14521 ttatttagtg aacaacctat ctcaaatgaa tcaggcttac ttatttcaat aggtaaaatt
    14581 gatgataact atttgtataa aacattaatt ggatcgattt ctaaggaaga aagttcaagt
    14641 atcgcaaatc aaatttttat caattaagga tgaaattggt taatccttct tagaaatttg
    14701 gagtattcca ataaaattag aaaggtattt tatggatact aatctttttt caaaaataaa
    14761 tacgacgatt tcggtaaaag aaaacgattt tattacagaa gaaaaatttc aaaaaattat
    14821 acaatccaaa gatacggaga cattggcatt tatcttagaa tcaactccct atcatttatc
    14881 gattgacatc ttagaagatc ctagtcagac agagatttcg ctaatgacaa aattagtcaa
    14941 tgattataga tgggcctatg ctgaaagtcc gtctgatata attgtgactt tatttgcttt
    15001 acgatatgtt tatcataata tcaaagtttt attaaaatct aaggcggcaa ttaagaaaga
    15061 tttttctaaa ttattaattc caatagggat ttttgatata gaaagtttaa aacatttagt
    15121 ttcttcctta cattcagata cacttcctga ttttatggtt cgtgaagtag aatcaatttg
    15181 gaatgagtat gaaactttta ataatattcg tgtacttgat gtcggagctg atctagcata
    15241 ttttaaacat ctgaaacttt tatctaatga gttagatgag gtactgtctc aggttattgt
    15301 cgaaatgatt gacttttata atattattac tgtaaaacgt ggtttatctc aaaataagag
    15361 tcatggggat attttacaat tactttcaga tgaaggaagt atttctgcta aagaatttat
    15421 atacattgta gaaaatcaag aaatatttgt gtggttcaat aaaataaatc caagcttaga
    15481 ttcaatcttt tcaacttatg aattgaagat gcaggacgca acaatttcat cttctgagtt
    15541 agaattttta tgtgatttac tattgtataa aactttagat caaggaaggt acaatgtaga
    15601 ggggccgtta gttcttgcta gatatttatt gggatgtgag tttgaagtaa agaatctcag
    15661 aatgatcata tcagctcttc aaaatacaat tccctttgaa tcaataaaag aaaggatacg
    15721 cccacattat ggaagctaat aagtataaaa ttggcataat tggtagccgt gatattattt
    15781 taccatttag catgattggg tttgatatat ttcctgccta ccaagaacaa gaagctataa
    15841 atacactaag aaaattagct caatctgatt atggtgtcat ttatatcact gaagacattg
    15901 cttcaatgat attagataca attcgccatt atgattccca agttgtgcct gctattattt
    15961 tattaccgac tcataaacaa ggtttaaatt taggattaaa acgtatagag gataatgtag
    16021 agaaagcagt aggacacaat attttataat aatgtacaaa attgtctgta atattattct
    16081 ataatttttg gacttagtaa ggagaataac tttgactcaa gggaagatta taaaagtatc
    16141 gggacctcta gttattgcat caggtatgca ggaggctaat attcaagata tttgccgtgt
    16201 aggtaagcta gggttaatcg gtgaaattat tgaaatgaga agagatcagg catctatcca
    16261 agtctatgaa gaaacatctg gtcttggtcc gggagaacct gttgttacaa ctggagaacc
    16321 tctctcggtt gaattagggc caggattgat ttctcaaatg tttgatggca tacaacgccc
    16381 attagatcga tttaaattgg ctactcataa tgattttcta gttcgtgggg tagaagttcc
    16441 aagtttggat agagatatta agtggcattt tgattccact atagcaattg gtcaaaaagt
    16501 gagtacgggt gatattcttg gaactgtcaa ggaaaccgag gtagttaatc ataaaattat
    16561 ggttccttat ggagtatctg gagaagtcgt ttctattgca tctggcgatt ttacaattga
    16621 tgaagttgta tatgaaataa aaaaattgga cggtagtttc tataaaggaa cgcttatgca
    16681 aaaatggcct gtccgcaagg cgcgtcctgt ttctaaacgt ttaattccag aagaaccatt
    16741 aatcacaggt caacgagtta ttgatgcatt ctttccagta accaaagggg gagctgcagc
    16801 agttcctgga ccgtttggag caggaaagac agttgtacaa caccaagtag ctaaatttgc
    16861 caatgttgat attgttattt atgtcggttg tggagaacgt ggaaatgaaa tgacggatgt
    16921 actgaatgag tttcctgagt tgattgaccc taataccgga caatcaatta tgcaacggac
    16981 agttctgatt gctaatactt caaatatgcc tgttgctgct cgtgaggctt caatttatac
    17041 aggaattacc atggctgagt attttcgtga tatgggctac tctgtcgcca ttatggctga
    17101 ttcaacttca cgttgggcag aagcgctacg tgaaatgtca ggacgtctag aagaaatgcc
    17161 tggtgatgag ggttatcctg cttatctggg aagtcgtatc gctgaatatt atgaaagagc
    17221 aggacgttct caggttctag ggcttccaga acgtgaagga acgattactg ctattggagc
    17281 tgtatcgcca cctggtggag atatttcaga accagttact caaaacactt tacggattgt
    17341 gaaagttttt tgggggcttg atgctccgtt ggcacagcga cgtcattttc ctgcaattaa
    17401 ctggcttaca tcttattcac tatataaaga cagtgtgggc acttatatag atggtaaaga
    17461 gaagacagat tggaatagta aaataactcg tgcgatgaac tacttacaac gggaatctag
    17521 tttagaggaa attgttcgtc ttgttggaat tgattctctg tctgataatg aacgactaac
    17581 gatggaaatt gctaaacaaa ttcgagaaga ttatttgcaa cagaacgctt ttgattcggt
    17641 agatacattc acttcgtttg caaaacaaga agcaatgcta agtaatattc tcacttttgc
    17701 tgatcaggca aatcatgctt tagagttggg ttcttacttt acagagatta tggaaggtac
    17761 cgtggcagtt cgagaccgta tggcgagaag taaatatgtt tcagaagata gattagatga
    17821 aatcaaaatt atatcaaatg agattacaca tcaaattcat ttgatattag aaacaggagg
    17881 tctataaatg agtgttataa aagaatacag aactgctagt gaagttgttg ggcctcttat
    17941 gattgttgaa caagtaaata atgtgtctta caatgagtta gttgaaattc aacttcataa
    18001 tggagaaatt cgtcgtggac aagttttaga gatccacgaa gataaagcaa tggttcagct
    18061 ttttgaagga tctagtggaa taaatttaga aaagtctaaa attcgttttg ctggtcatgc
    18121 attagaattg gctgtatctg aggatatggt tggtcgtatt tttaatggga tgggaaaacc
    18181 aattgatggt ggaccagatt taattccaga gaaatattta gatattgatg gtcaagctat
    18241 taatcctgta tctagagatt atccagatga atttattcag acagggatct cctctattga
    18301 tcatttgaat actcttgtac gtggtcaaaa attaccagta ttttcaggtt cgggcttacc
    18361 tcataatgaa ttagctgctc agatagcaag acaagcgact gttttaaatt ctgatgaaaa
    18421 ttttgcggtt gtatttgcag caatgggtat tacttttgaa gaagctgagt tttttatgga
    18481 agaactcaga aaaacaggag cgatcgatcg ttcggtttta tttatgaact tggcaaatga
    18541 tcctgcaatt gagcgtattg caactccccg cattgcttta actgcggcag agtatctagc
    18601 ttttgaaaaa gatatgcacg ttctagttat catgacggat atgactaact attgtgaagc
    18661 gttacgtgaa gtctcggcag ctcgccgtga agttccaggg agacgaggct atccgggata
    18721 tttatataca aatttatcaa ctctatacga aagggctggt cgcttagttg gtaaaaaagg
    18781 ttcggtgaca cagattccta ttttaacaat gccagaagat gacataacac atccaattcc
    18841 tgatttaact ggatacatta ctgaagggca aattattttg tcgcatgagt tgtataatca
    18901 aggttatcgt ccaccaatca atgttttacc ttctctctct cgattaaaag ataagggatc
    18961 tggagaaggt aaaactcgtg gagatcatgc tccaactatg aatcaactgt ttgcagccta
    19021 tgcccaaggg aaaaaggttg aagagttagc agtagtatta ggagaatcgg ctttatctga
    19081 tgtagataaa ttgtatgtga ggtttacaaa gcgttttgaa gaagagtaca taaaccaagg
    19141 attttataaa aatcgaaata tagaagatac gttgaatctt gggtgggaat tactatcaat
    19201 tcttcctaga acagagttaa aacgtatcaa agatgatttg cttgataaat acttaccttt
    19261 ggtagaagtt taatccggaa atggagtgat tatctatggt acgtttgaat gtaaaaccaa
    19321 ctcgtatgga attgaataac ttaaaggaac gtttgacaac agctgaacgt ggacataagt
    19381 tattaaagga taaaagagat gaattgatga ggcgatttat ttctttgatt cgtgagaata
    19441 atcaacttcg gaaagaagtg gaaagttatc taattgataa tctaaaatcc tttgcagttg
    19501 ctaaatcatt aaagaattct caaatggtgg aggaattatt ttcaattcca tcgaaagaaa
    19561 ttgaattatt tgttgagaaa gaaaatatca tgagtgtaac agttcctaga atgcatatga
    19621 atattacttc tcaaaatgag aacagtgaat acagctattt atcttctaat agtgaaatgg
    19681 atgatgtatt tgctacaatg aatagtttaa tttataaatt actaagactg gcagaagttg
    19741 aaaaaacgtg tcagttaatg gctgatgaaa tagaaaaaac acgtagacgt gtaaatggtt
    19801 tagaatactc gattattcca aacttgtcgg aaactattca ttatatagaa ttgaaactag
    19861 aggaggcaga aagagccaat ttagttcgta ttatgaaagt gaagtagatc ctttatttag
    19921 attattaatt agatgaacaa atatcagctt ggataaggct ttaagccttt ctaagctttt
    19981 tttattgaca gtatcaggat atctttttca aaattttggt ttgttagata atgaaaatgt
    20041 ttctactaat ctagatttag gattagtaaa tcgtaaatgt aattatatag aaagtaagcg
    20101 cgtcataaca aggtatctat cattcatgga gctcctcctg tatactatta gtaaagtaaa
    20161 actattggag gatattttaa tgccacaacc tattgttcct gta
//
LOCUS       DBDESG010000046        20071 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.046, whole genome shotgun sequence.
ACCESSION   DBDESG010000046 DBDESG010000000
VERSION     DBDESG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.046"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            72..683
                     /locus_tag="ACKB38_001556"
     CDS             72..683
                     /locus_tag="ACKB38_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGL7980304.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            844..1638
                     /gene="ccrZ"
                     /locus_tag="ACKB38_001557"
     CDS             844..1638
                     /gene="ccrZ"
                     /locus_tag="ACKB38_001557"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGL7980305.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            1635..2270
                     /gene="trmB"
                     /locus_tag="ACKB38_001558"
     CDS             1635..2270
                     /gene="trmB"
                     /locus_tag="ACKB38_001558"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGL7980306.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            2396..2875
                     /gene="rimP"
                     /locus_tag="ACKB38_001559"
     CDS             2396..2875
                     /gene="rimP"
                     /locus_tag="ACKB38_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGL7980307.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            2919..4055
                     /gene="nusA"
                     /locus_tag="ACKB38_001560"
     CDS             2919..4055
                     /gene="nusA"
                     /locus_tag="ACKB38_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGL7980308.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            4077..4370
                     /locus_tag="ACKB38_001561"
     CDS             4077..4370
                     /locus_tag="ACKB38_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGL7980309.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            4363..4662
                     /locus_tag="ACKB38_001562"
     CDS             4363..4662
                     /locus_tag="ACKB38_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGL7980310.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            4679..7555
                     /gene="infB"
                     /locus_tag="ACKB38_001563"
     CDS             4679..7555
                     /gene="infB"
                     /locus_tag="ACKB38_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGL7980311.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            7806..8156
                     /gene="rbfA"
                     /locus_tag="ACKB38_001564"
     CDS             7806..8156
                     /gene="rbfA"
                     /locus_tag="ACKB38_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGL7980312.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTIIDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            8435..9214
                     /locus_tag="ACKB38_001565"
     CDS             8435..9214
                     /locus_tag="ACKB38_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980313.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRVGSITIAK
                     QVMKIVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            9216..9398
                     /locus_tag="ACKB38_001566"
     CDS             9216..9398
                     /locus_tag="ACKB38_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980314.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9675..9827)
                     /locus_tag="ACKB38_001567"
     CDS             complement(9675..9827)
                     /locus_tag="ACKB38_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980315.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEVSSKHRFEVVDRTDEVS
                     NIYTAR"
     gene            9858..10091
                     /locus_tag="ACKB38_001568"
     CDS             9858..10091
                     /locus_tag="ACKB38_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGL7980316.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            10091..11425
                     /locus_tag="ACKB38_001569"
     CDS             10091..11425
                     /locus_tag="ACKB38_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGL7980317.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            11435..11689
                     /locus_tag="ACKB38_001570"
     CDS             11435..11689
                     /locus_tag="ACKB38_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGL7980318.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            11785..12180
                     /locus_tag="ACKB38_001571"
     CDS             11785..12180
                     /locus_tag="ACKB38_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGL7980319.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            12593..13042
                     /locus_tag="ACKB38_001572"
     CDS             12593..13042
                     /locus_tag="ACKB38_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HGL7980320.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            13029..13589
                     /locus_tag="ACKB38_001573"
     CDS             13029..13589
                     /locus_tag="ACKB38_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGL7980321.1"
                     /translation="MARTELPDKIETERLVLRVRTVVDAEDIHTYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERTQKENLPAGYGIVVKGTDKVIGSVDFNHLHADDVLELGY
                     TLHPDYWGRGYVPEAACSLIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDAQGNCCDDLRYGLLRSEWEVI"
     gene            13589..14167
                     /locus_tag="ACKB38_001574"
     CDS             13589..14167
                     /locus_tag="ACKB38_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGL7980322.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQDLLDNFHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQHQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            14160..16811
                     /locus_tag="ACKB38_001575"
     CDS             14160..16811
                     /locus_tag="ACKB38_001575"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGL7980323.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            16892..17056
                     /locus_tag="ACKB38_001576"
     CDS             16892..17056
                     /locus_tag="ACKB38_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGL7980324.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            17107..18711
                     /locus_tag="ACKB38_001577"
     CDS             17107..18711
                     /locus_tag="ACKB38_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGL7980325.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            18732..18873
                     /locus_tag="ACKB38_001578"
                     /pseudo
     CDS             18732..18873
                     /locus_tag="ACKB38_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155197.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            19091..19894
                     /locus_tag="ACKB38_001579"
     CDS             19091..19894
                     /locus_tag="ACKB38_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGL7980326.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPAILLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDLS"
BASE COUNT         6253 a         3682 c         4508 g         5628 t
ORIGIN      
        1 tggtttgttt tggaaaggct agaactaaag aatgacgtgt aggaagtctg tttgttgagg
       61 aggataattt tatggagttt tttgataaat ttcatgcctt gtgttttgga tttttagtac
      121 taataattgt cattacagtt ccttatacga ttaaccatgg gggttttttt caaaatgaat
      181 ctgcattgat tcttgtaagt cttcttgtaa cctcgctgag tgttgcttat gctagaaagt
      241 ttgaaatgat ttcttttggg atgttaagca agaaacaact tttgcttttc attgcaatct
      301 ttcttctaag tgtacttgag acgctggttt atattcattt cttcgctgtt tcttctggct
      361 caggggtcca acacttggcg gaagtcagca gaggaatttc cctgtctttg attttgacta
      421 cctcagtttt tggccccatc caggaggaac tcattttcag aggacttctt caaggtgcgg
      481 tttttgacaa ttcttggtta gggcttgtgc taacttcctc tctcttttct ttcatgcatg
      541 gaccttctaa tgtcccttcg tttatttttt atctacttgg gggtttgttg ctgggctttg
      601 cttataaaaa gagtcaaaac ctatgggttt ctactctagt ccatatgctt tacaacagtt
      661 ggccactctt atattattta taaaaattat gaaaaggtaa gcagaagacg gtgcttacct
      721 ttttcaagct ataatgatac ccaagccaat ccagaggctt ttctttgaga atcgggttag
      781 agtggttgac gaataggcca aaaactagta gaatagtaag gaaactttat acggaggaaa
      841 gaaatggatt tgggtgataa tgagctaaca ctgactccca tacctgggaa aagtggcaag
      901 gcttatatgg gtagctatcc tgatgggaag cgcatctttg taaaaatgaa cacctctcca
      961 atcctacctg gtctagctag agaacaaatt gctccacaat tattatggag tcgtcgtttg
     1021 gcagatgggc gtgatatgtg tgctcaagaa tggttgacag gcaagatatt gaccccctat
     1081 gatatgaatc gtaagcaaat cgtcaatatt ttaacccgtc ttcatcgctc acgtccgttg
     1141 atgacacaat tgagtcgttt gggctatgcc atggaaacac ctgtagattt actacagtct
     1201 tggcaggaaa cggctccaga tgctttgcgt aaaaatcatt ttatcagtga agtgatggct
     1261 gatttacgtc agactattcc aggatttaga gaggaccatg cgaccattgt ccatggagat
     1321 gtacgacata gtaattggat tgagacagat agtggcttga tttatttagt agattgggat
     1381 tcggttcgct tgaccgatcg catgtttgat gtggcccata tgctctgcca ttatatttca
     1441 gaacatcagt ggaaggaatg gttgacctac tacggttaca agtacaatca aacggtatta
     1501 agtaaattgt attggtatgg tcaattgtct tatttgagtc agatttccaa gtattatatg
     1561 aaccaagatt tagaaaatgt caatcgggag attcatggtt tgcgtcattt ccgagacaag
     1621 tatggaaaga gaagatgaga gttagaaatc gtaaaggggc aacagaatta ctagaggcaa
     1681 atccccagta tgtggtcctc aatcccttgg aagccaaggc aaaatggcgg gacttgtttg
     1741 gcaatgataa tcccattcat gtggaagttg gaagtggaaa gggtgccttt gtttcaggta
     1801 tggccaagca aaaccctgac atcaactata tcgggattga tattcaaaag tctgttttga
     1861 gctacgcttt ggacaaggtg cttgaagttg gagtgcctaa catcaagctc ttgtgggtag
     1921 atggttctga cttaactgac tactttgaag acggtgagat tgatcgcttg tatctgaact
     1981 tttcagatcc atggccgaaa aaacgccatg aaaagcgtcg tttgacctac aagaccttct
     2041 tggatacctt caaacgtatc ttgcctgaaa atggagaaat tcatttcaag acggataacc
     2101 gtggcttgtt tgagtacagt ttagtgagct tttctcaata tggcatgaaa ctcaatggtg
     2161 tctggttaga tttgcatgcc agtgattttg aaggcaatgt catgacagaa tacgagcaaa
     2221 aattctcaaa caaggggcaa gttatctacc gagttgaggc agaattttaa gagataacct
     2281 aaaattaggc tgtacaagtg cttttgcttt acataagttg gcaaacgtgc tatactgata
     2341 gtaagaatat gaaaagtgag gcggggaaat atcttcgcct cttgcttatg aggaggtgga
     2401 cgcaatcgca acaatcgtag aattagtcag agaagttgta gaacctgtca tagaagctcc
     2461 ttttgaactc gtggatatcg agtatggaaa gattggcagt gacatgattc tcagtatttt
     2521 tgtagataaa cccgaaggaa ttaccttgaa cgacacggca gacttgacag aaattatcag
     2581 tcctgtccta gacaccatca agccagatcc cttcccagaa caatatttcc tagaaattac
     2641 cagtccaggt ttggaacgtc ctttgaaaac caaggatgcc gtcgctggag cggttggaaa
     2701 atacatccat gtcgggctct accaagccat cgataagcaa aaggtctttg aaggaacctt
     2761 gttggccttc gaagaggacg agttgactat ggaatatatg gacaagacgc gtaagaaaac
     2821 cgtccaaatt ccatacagtt tagtatcaaa agcacgttta gcagttaaat tatagaaaaa
     2881 gaaaggatag cttttgagga ttcaaaagtg aagaaaacat gagtaaagaa atgctagagg
     2941 ccttccgcat tttggaagaa gacaagggaa tcaaaaaaga agatatcatc gacgcagtag
     3001 tagagtcgct tcgttccgct tatcgcagac gctatggtca gtcagacagc gtagctattg
     3061 acttcaacga aaaaacaggt gactttacag tttatactgt ccgtgaagtt gttgatgaag
     3121 tatttgatag ccgtttggaa atcagcttga aagatgctct tgccattaat tcagcttatg
     3181 aacttggaga caaaatcaag tttgaagaag caccagctga gtttggtcgt gtagcagccc
     3241 aatctgccaa acaaaccatc atggaaaaaa tgcgcaagca aacacgtgcc atcacttaca
     3301 atacttacaa agaacatgag caagaaatca tgtctggtac agtagaacgc tttgacaacc
     3361 gctttatcta tgtcaacctt ggtagcatcg aagcccaatt gtcaaaacaa gaccaaattc
     3421 ctggagaagt ttttgcttct catgatcgta tcgaagttta tgtttacaag gttgaagaca
     3481 accctcgtgg tgtgaacgtc tttgttagcc gtagtcatcc agaaatgatc aaacgtttaa
     3541 tggagcaaga aattccagaa gtttatgatg gaactgttga aatcatgagc gtggctcgtg
     3601 aagcaggtga ccgtacgaag gttgctgttc gtagccacaa tccaaacgtg gatgctatcg
     3661 gtacaatcgt tggacgtggt ggtgctaata tcaagaagat tactagcaaa ttccacccag
     3721 ctcgttacga tgctaaaaat gaccgcatgg taccaatcga agaaaatatc gatgttatcg
     3781 agtgggtagc agatccagct gaatttatct acaatgccat cgctcctgct gaggttgacc
     3841 aagttatctt tgatgaaaac gacagcaaac gtgccttggt ggttgttcca gataacaagc
     3901 tttctcttgc cattggtcgt cgtggacaaa acgtgcgctt ggcggctcac ttgactggtt
     3961 accgtatcga tatcaagtct gctagcgaat ttgaagccat ggaagacgct gcttcagtag
     4021 agttggaagt agaaaacgat actgtagaag aataaaagct gctagaggag ggaaagatga
     4081 aaacaagaaa aatccctttg cgcaagtctg ttgtgtctaa cgaagtgatt gataagcgtg
     4141 atttgctccg cattgtcaag aacaaggaag gacaagtctt tattgatcct acgggcaagg
     4201 ccaatggccg cggcgcttat atcaaactag acaatgcaga agccctagag gcgaaaaaga
     4261 agaaggtctt taaccgcagc tttagcatgg aagtggaaga aagcttttat gacgagttga
     4321 tcgcttatgt ggatcacaaa gtgaaaagaa gagagttggg acttgaataa gcaaaagata
     4381 agtaatctct tggggcttgc tcagcgagca gggcgcatca tatcgggtga agaattggtg
     4441 gtcaaggcca ttcaagacgg caaggccaag ttggtctttc tagctcatga tgctggaccc
     4501 aatctgacca agaagattca agataaaagt cattattatc aagtagaaat tgtaaccgtg
     4561 ttttcaacac tggaattaag catagcagtc gggaaatcga gaaaggtttt ggctgtaaca
     4621 gatgctggat ttacaaagaa aatgaggtct cttatggaat agaagaggag gacatgattt
     4681 gtctaagaaa agattgtacg aaatcgcaaa agaacttgga aaagaaagta aagaagttgt
     4741 agcgcgtgca aaagagttgg gcttggatgt gaaaagccac tcatcaagtg tggaagaagc
     4801 tgtcgctgca aaaattgctg ccagctttaa gcctgcagct gctccgaaag tagaagcaaa
     4861 acctgcagcc ccaaaagtaa gtgcagaaaa gaaagccgaa aaatctgagc cagctaaacc
     4921 agctgtagct aaggaagagg caaaacctgc agccccaaaa gcaagtgcag aaaagaaagc
     4981 cgaaaagtct gaaccagtaa aaccagctgt agccaaggaa gaggcaaaac cagctgagcc
     5041 agtcactccg aaaacagaaa aagtagcggc taaaccgcaa agtcgtaatt tcaaggctga
     5101 gcgtgaagca cgtgctaaag agcaggcaga gcgacgcaag caaaataagg gcaataaccg
     5161 tgaccaacaa caaaacggaa accgtcagaa aaacgacggc cgtaatggtg gaaaacaagg
     5221 tcaaagcaac cgcgacaatc gtcgctttaa tgaccaagct aagaagcagc aaggtcagca
     5281 aaaacgtaga aatgagcgcc gtcagcaaga ggataaacgt tcaaatcaag cggctccacg
     5341 tattgacttt aaagcccgtg cagcagccct aaaagcagag caaaatgcag agtacgctcg
     5401 ttcaagtgag gaacgcttca agcagtatca ggctgctaaa gaagccttgg ctcaagctaa
     5461 caaacgcaag gaaccagagg aaatctttga agaagcggct aagttagctg aacaagcaca
     5521 gcaagttcaa gcagtggttg aagtcgtccc tgagaaaaaa gaacctgcag tggatacacg
     5581 tcgtaaaaaa caagctcgac cagacaaaaa tcgtgacgat tatgatcatg aagaagatgg
     5641 tcctagaaaa caacaaaaga atcgaagtag tcaaaatcaa gtgagaaatc aaaagaatag
     5701 taactggaat aacaacaaaa agaacaaaaa aggcaataac aagaacaacc gtaatcagac
     5761 tccaaaacct gttacggagc gtaaattcca tgaattgcca acagaatttg aatatacaga
     5821 tggtatgacc gttgcggaaa tcgcaaaacg tatcaaacgt gaaccagctg aaattgttaa
     5881 gaaacttttc atgatgggtg tcatggccac acaaaaccaa tccttggatg gggaaacaat
     5941 tgaactcctc atggtggatt acggtatcga agccaaacaa aaggttgaag tggataatgc
     6001 tgacatcgaa cgtttctttg tcgaagatgg ttatctcaat gaagatgaat tggttgagcg
     6061 tccaccagtt gttactatca tgggacacgt tgaccacggt aaaacaaccc ttttggatac
     6121 tcttcgtaac tcacgtgttg cgacaggtga agcaggtggt attactcagc atatcggtgc
     6181 ctaccaaatc gtggaaaatg gtaagaagat taccttcctt gatacaccag gacacgcggc
     6241 ctttacatca atgcgtgcgc gtggtgcttc tgttaccgat attacgatct tggtcgtagc
     6301 ggcagatgac ggggttatgc ctcagactat tgaagccatc aaccactcaa aagcagctaa
     6361 cgttccaatc atcgtagcta ttaacaagat tgataaacca ggtgctaacc cagaacgcgt
     6421 tatcggtgaa ttggcagagc atggtgtgat gtcaactgct tggggtggag attctgaatt
     6481 tgttgaaatt tcggctaaat tcaaccaaaa tatcgaagaa ttgttggaaa cagtccttct
     6541 tgtggctgaa atccaagaac tcaaagcaga cccaacagtt cgtgcgatcg gtacggttat
     6601 cgaagcgcgc ttggataaag gaaaaggtgc ggtcgcaacc cttcttgtac aacaaggtac
     6661 cttgaatgtt caagacccaa tcgttgtcgg aaataccttc ggtcgtgtcc gtgctatgac
     6721 caacgacctt ggtcgtcgtg ttaaagttgc tggaccatca acaccagtct ctatcacagg
     6781 tttgaacgaa gcaccgatgg cgggtgacca ctttgccgtt tacgaggatg aaaaatctgc
     6841 gcgtgcagca ggtgaagagc gtgccaaacg tgccctcatg aaacaacgtc aagctaccca
     6901 acgtgttagc cttgaaaacc tctttgatac ccttaaagct ggggaactca aatctgttaa
     6961 tgttatcatc aaggctgatg tacaaggttc tgttgaagcc ctttctgcct cacttcaaaa
     7021 gattgacgtg gaaggtgtca aagtgactat cgtccactca gcggtcggtg ctatcaacga
     7081 atcagacgtg acccttgccg aagcttcaaa tgcctttatc gttggtttca acgtacgccc
     7141 tacaccacaa gctcgtcaac aagcagaagc tgacgatgtg gaaatccgtc ttcacagcat
     7201 tatctacaag gttatcgaag agatggaaga agctatgaaa gggatgcttg atccagaatt
     7261 tgaagaaaaa gttattggtg aagcggttat ccgtgaaacc ttcaaggtgt ctaaagtggg
     7321 aactatcggt ggatttatgg ttatcaacgg taaggttgcc cgtgactcta aagtccgtgt
     7381 tatccgtgat ggtgtcgtta tctatgatgg tgaactcgca agcttgaaac actataaaga
     7441 cgacgtgaaa gaagtgacaa acggtcgtga aggtggattg atgatcgacg gctacaatga
     7501 tattaagatg gatgatgtga ttgaggcgta tgtcatggaa gaaatcaaga gataagattt
     7561 tttgctcctt tcttaggtgg tgagggacgc aagcaaaccg atggtttcat tgcttatttt
     7621 tgagcctagg gtctcaaaaa tcccctgtga tgggactgat aaatcagttc catcactttc
     7681 accacggcga aagaagcaga tgacttcaaa ttgaacttcg tttcaattta aactgaaaat
     7741 caagaagttt aaaatagcta ggtctgctgg cctagctttt ggttcaaagt agagaaagga
     7801 atatcatggc aaatcatttc cgtacagatc gtgtgggcat ggaaatcaag cgtgaagtca
     7861 atgagatttt gcaaaagaaa gtccgtgatc cacgtgtcca aggtgtgacc atcatagatg
     7921 ttcagatgct gggtgacttg tctgttgcca aggtttatta caccattttg agtaaccttg
     7981 cttcggataa ccaaaaagcc caaatcgggc ttgaaaaagc aactggtacc atcaaacgtg
     8041 aacttggtcg caatttgaaa ttgtacaaaa tcccagattt gaccttcgtc aaagacgagt
     8101 ccatcgagta tggaaacaag attgacgaga tgctacgcaa tctggataag aactaaagaa
     8161 gaggggttgc ccctcttttt tggtggagga aaataggttg aatttgaaat ggaaaaatat
     8221 tcttttataa tagattgaaa ctagaatagt acgcctctac ttctaaaata ttgttagaaa
     8281 tcgatttgac tgtcctgatc gatttgtcct gttcttgttt cattttaata taaaaaaggg
     8341 attctgtatt ttttaatgtt atctaattag aaaatgcttt ttttgtagga aatataatat
     8401 gataaggtgc aaaaaagaaa taaggagttt gtatatggct gaacaagact tagctatgca
     8461 agtattgcaa caagtggtga aactacctgt tgttaaggtt gatcgttcga aatttttagt
     8521 ggataagttt tccaaagaat tggatccaaa agatattcct accttattgg aacaaggtcc
     8581 aacgactctt ctatctcaag aaatattaga tcgtgtagct aatgcttgta ttcgggacaa
     8641 tgtattatta gcgagtggga cttctgtttt ggcaggatta cctggagggc ttgctatggc
     8701 aattaccatt ccagctgatg tggctcaatt ttatgctttc tctctgaaat tggctcaaga
     8761 attaggttat atttatggtt atgaggatct ttgggcttca cgagaggagt tgagtgaaga
     8821 tgctcaaaat accctcttgc tttatctagg cgtaatgtta ggggtgaatg gaaccgctgc
     8881 tttgctacgt gttggtagta taacaattgc caaacaggta atgaaaatag tgcctaataa
     8941 agctttaaca aagacgcttt ggtaccctat tttgaaaaaa gtcttaaaaa tatttggtgt
     9001 gaatcttacc aagggagggt tggccaaagg aatggggaaa tttattccta tcttgggtgg
     9061 tatcatttca ggtggtttaa cctttgcaac tatgaaacca atgggggaaa gcttgcagaa
     9121 agaattatcc aagctagtca actatagtga agttcaatat caagaagatg ttgaaacaat
     9181 ccgaaaagag gctgaaatca tcaaaggaga gtaatatgaa tcctatcaaa gcttttgcta
     9241 aaatttatgg taattacttt ttgaccgtgc aaggtgtaaa agtgatgaaa acgataaaga
     9301 aagctgacca tgtcgttgtt ggtctgggga aactttttat tgccgacaag ttaatggata
     9361 cggctcggtg gctcattaag ccagaggaga gagaatgaaa tttttttggt cttcttgcta
     9421 ttctttttat caaaccgatt attgggattg tgaaattctt ttggatgatc atctcttttg
     9481 cagtccaatt gctgttttac aagatagtgt ttaagatatt ggattggctc tttaaactta
     9541 tctagatggt aatccaagtt gcagagaact agcaggaact ccactgctag ttttttattc
     9601 tctttccata tggtataata taagcagtaa aatcatttta tactcttcga aaatctcttc
     9661 aaaccacgtc agcttcacct tgcagtatat atgttactga cttcgtcagt tctatccaca
     9721 acctcaaaac ggtgttttga gctgacttcg tcagttctat ctacaacctc aaaacactgt
     9781 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtattagaac
     9841 atacaatgga ggtcgtcatg gacaatatca tcgatgtgtc aattcctgtt gcagaagtgg
     9901 tggacaagca tccagaagtc ttggaaattc tagtggagtt gggttttaaa ccccttgcca
     9961 atcccttaat gcgcaataca gttggtcgta aagtatcact taaacagggt tctaagctag
    10021 caggaactcc tatggacaag attgtacgca cactggaagc gaatggctac gaagtgattg
    10081 gattagacta atgacagatg aacggattca tatcctacgg gatattttgt tagaattgca
    10141 caatggcgcc tctcctgagt cggttcaaga tcgctttgat gcgaccttta cgggcgtgtc
    10201 agccatcgag atttccctta tggagcacga gctgatgaac tcggattcgg gcgtcacttt
    10261 tgaagatgtt atggaactct gtgatgtcca tgccaatctt tttaaaaatg ctatcaaagg
    10321 tgtcgaagtt tcagatactg agcatccagg tcacccagtt cgtgtcttca aagaagaaaa
    10381 tctggctctc cgtgcggcct tgattcgcat tcgtagattg ttagatacct atgagtctat
    10441 ggaagacgag gaaatgctgg cggagatgcg taagggtttg gtgcgtcaga tgggacttgt
    10501 gggtcaattt gacatccatt accaacgtaa ggaagaactc ttctttccta tcatggagcg
    10561 ctatggacac gattcacctc ccaaagttat gtggggagtg gatgatcaga ttagggaact
    10621 ctttcaaaca gctctaacga cagccaagtc actaccagaa gtgtcaatta gcagtgtaaa
    10681 ggaagctttt gaagcttttg cgacagagtt tgaaagtatg attttcaagg aagagtccat
    10741 cctcctcatg attctccttg agtcttttac tcaggatgac tggcttcaga ttgcggagga
    10801 gagcgatgcc tatggctatg ccatcatccg tccgtcagag aaatgggtgc cagaacgaca
    10861 gagctttatt gaggaaaaga ttgcagagga gcctgtacag ctagatacgg cagaaggtca
    10921 agttcaacaa gtcatagata cgccagaagg ccattttacc attaccttta cccctaagga
    10981 aaaggaagct gtgctggacc gccatagtca acaggctttt ggtaatggct atctttcagt
    11041 cgagcaggcc aatctcatcc tcaatcatct ccctatggag attacctttg tcaataaaga
    11101 agatattttc cagtattaca atgacaatac gccagctgat gagatgattt tcaaacggac
    11161 gccgtcccaa gtcgggcgca atgtcgaact ctgccatccg cctaagtact tggacaaggt
    11221 caaaactatc atgaaggggc ttcgtgaggg aagcaaagac aagtatgaaa tgtggttcaa
    11281 gtctgagtcg cgaggtaagt ttgtccacat cacctatgct gcagtacacg atgaagacgg
    11341 agaattccaa ggagtgttgg agtatgttca ggatatccag ccctaccgtg agattgatac
    11401 ggactatttt cgtggattag aataaggaga aaaaatgagt tacgaacaag aatttatgaa
    11461 ggaatttgaa gcttgggtca atacccaaat catgattaac gacatggcgc acaaggaaag
    11521 ccaaaaagtt tacgaagaag accaggacga gcgtgccaaa gatgccatga ttcgctacga
    11581 gagtcgcttg gatgcttatc agttcttgct tggtaagttt gaaaacttca aagtaggcaa
    11641 gggattccat gatttgccag aaggcttgtt tggtgagcga aattattaaa cgagaaagat
    11701 tcttgatttt tcactaaaat cttgatagaa tgtttatgtt aaatccttgt cagagcaggg
    11761 attttttatt gaaaggattt tatcatgtca aagaaactca atcgtaaaaa acaattacga
    11821 aatggcctcc gtcgcgcagg tgccttttca agtacggtga ctaaggttgt agatgagaca
    11881 aaaaaagtcg tgaagcgtgc agaacagtca gcaagcgcag ctggtaaggc tgtttctaaa
    11941 aaagttgaac aagcagtaga agctaccaaa gagcaagctc aaaaagtagc taattctgta
    12001 gaagattttg cagcaaattt gggtggactt ccacttgatc gtgccaagac tttctatgat
    12061 gaaggaatca agtctgcttc agatttcaaa aactggactg aaaaagaact ccttgccttg
    12121 aaaggaatcg gcccagctac catcaagaaa ttgaaagaaa atggcatcaa gttcaagtaa
    12181 tttttcttga gccttgcatt tccgaaaaaa tcttgctaca atagagccat tagaggtgtt
    12241 ttgaatccca cattttacag aaagtggcgg cgctgagaag tccacaaatg tgtcaaaact
    12301 ggttgctaat ggatgaaaaa ttgaaataaa agtgtctttt tgctttaaag acgagagttg
    12361 cgggcaactg cccgaaattg ggtggtaccg cggataaaca cattcgtccc tgtcatgtag
    12421 atggcagggc ttttctttta cgtcttagtc aaagggggtt gttatgaagc aatcttttaa
    12481 cacaagtaaa ctatattatg gttttcctgt ttttatttta ggatatcaag attagaattt
    12541 tggctataac atcacgacct gtagttcctc ttatagtcta ggagattggg ttgtgattgg
    12601 agtcgttgcg agagagaatg ccgcagagca gatcaaacag tatcaaaaat ttactgtgaa
    12661 tatttctgat gaaacttcta tgcttgcgat ggagcaggct ggttttatca gtcatcagga
    12721 gaaattggaa cgtttgggag tgcattatga aatttctgaa cgaactcaga ctcctatttt
    12781 agacgcctgt ccacttgttt tagattgtcg ggtagatagg attgttgagg aagacggtat
    12841 ttgccacatc tttgccaaga ttcttgagcg acttgttgcc ccagaactcc tggatgaaaa
    12901 gggacatttt aaaaatcaac tgtttgcccc aacctatttc atgggagatg gatatcagcg
    12961 cgtttatcgc tatctggaca agcgtgtaga tatgaagggc agtttcatca aaaaagcgag
    13021 gaagaaggat ggcaagaact gagctgccag ataaaatcga aacagaacgt ctcgttttac
    13081 gagtccgcac agtggtggat gctgaggata tccataccta cgctagtttg ccagaggtcg
    13141 cctatccagc aggttttcct ccagtcaaga tcttggaaga tgagatttat tatctggagc
    13201 acattcttcc ggagcgtact caaaaggaaa atctcccagc tggctatggg attgtcgtca
    13261 aaggaaccga taaagtcatt ggttctgttg acttcaacca tcttcatgcg gatgatgtgc
    13321 tagagcttgg ctataccttg cacccagact attggggtcg aggttatgtt ccagaagcag
    13381 cgtgttcctt gattgatcta gcttttaaag aacttggtct tcacaagatt gaactaactt
    13441 gctttggata taaccttcaa agtcaacgag tctcggaaaa gcttggcttt accctcgaag
    13501 ctcgcataag agaccgcaaa gatgctcaag gcaactgctg tgatgatttg agatatggct
    13561 tgctgaggag tgagtgggag gtgatttgat gcatcttttc atcattggtg ctccagcatc
    13621 aggaaaaatg accattggtc aagaattatc tcgactgacg gatgctaccc tattttataa
    13681 ccatcaagcc attgattttg cactagagat ctatcaggat tataccgagg agatgtggga
    13741 atttgttcgt ggaattacct tttctttcct tggagcaagt gcaagaaatc agcgatcagt
    13801 aattttaaca gacgtaattg atttttcaaa tcagtaccag ctgatgtatt taaagcaaat
    13861 tcaggattta ttggataact ttcatcaaga gattctgttt gttgaattgg aaacaagtct
    13921 tgaagagtgc atacgtcgaa atcgaacgga gaatcgatta aagcacaaac ccttaaaacg
    13981 acatattgag gtatctgaaa gagaaatttt agagaccgct gaaacacttc aattaaattc
    14041 ccaacatcaa ccgaatgagt tgcaccacta ctttaaaata aataatacga atttgtctgc
    14101 agaagaagct gctaagcaaa ttcaaaataa aataaacaaa atagagaaag gacacacaca
    14161 tgtctaaaga actttcacct aaatacaatc cagccgaggt tgaggctggt cgttaccaaa
    14221 aatggcttga tgcggatgtt ttcaagcctt caggcgatca aaaggctaag ccttattcaa
    14281 tcgtgattcc accaccaaac gtaactggga aacttcacct tggtcacgct tgggatacga
    14341 ctttgcagga tatcatcatc cgtcaaaaac gcatgcaagg ctttgatacc ctttggcttc
    14401 caggtatgga ccacgctggt attgcgactc aggctaaggt tgaggagcgc ttacgtggtg
    14461 agggtattac gcgttatgac ctaggtcgtg agtctttctt gacgaaggtc tgggaatgga
    14521 aagacgaata tgccactacc atcaaggaac aatggggcaa gatggggctt tctgtagact
    14581 attctcgtga gcgtttcact cttgatgaag gtttgtcaaa agctgttcgt aaggtctttg
    14641 tgaaccttta caagaaaggc tggatctatc gtggtgagtt tatcatcaac tgggacccag
    14701 cagctcgcac agccctttct gatattgagg tgattcacaa ggatgtagaa ggtgccttct
    14761 accacatgaa ttacatgctg gaagatggtt cacgcgccct tgaagttgct acaactcgtc
    14821 ctgagactat gtttggggac gttgcggttg cggttaatcc agaagacccg cgctacaagg
    14881 acttgattgg taaaaacgtc atccttccaa tcgctaataa actcatccca atcgttggag
    14941 atgagcacgc agatcctgag cttggtactg gtgtcgtgaa aatcacacct gcccacgatc
    15001 caaatgactt cttggttggc caacgtcata acttgccaca agtcaacgtc atgaacgacg
    15061 acggaactat gaatgagctt gcctttgaat tttcaggcat ggatcgtttt gaagctcgta
    15121 aggcagtcgt tgctaagttg gaagaaatcg gtgcccttgt caaaatcgaa aaacgtgtcc
    15181 acagtgttgg tcactcagaa cgtacaggtg tcgtagttga gccacgcttg tctactcaat
    15241 ggttcgtcaa gatggaccaa ttggctaaga acgccattgc taaccaagac acagaggaca
    15301 aggtcgaatt ctacccacct cgtttcaacg ataccttcct tcaatggatg gaaaatgtcc
    15361 acgactgggt tatctctcgt cagctttggt ggggtcacca aatccctgcc tggtacaatg
    15421 ctgatggtga aatgtatgtc ggcgaagaag ctccagaagg tgacggatgg actcaggacg
    15481 aagacgtctt ggatacttgg ttcagttctg ccctctggcc attttcaacc atgggctggc
    15541 ctgaagtcga ctcagaagac tttaaacgtt atttcccaac ttcaaccttg gtaacaggtt
    15601 acgacatcat cttcttctgg gtgtctcgta tgatcttcca gtccttggaa tttactggtc
    15661 gtcagccatt ccaaaacgtg cttatccacg gtctcatccg tgacgagcaa ggacgcaaga
    15721 tgtctaaatc tctcggtaac gggattgacc caatggatgt catcgagaaa tacggtgctg
    15781 atgcccttcg ttggttcctt tcaaacggtt ccgcaccagg tcaagacgtt cgtttctctt
    15841 acgagaaaat ggatgcttca tggaacttta ttaacaaaat ttggaacatt tctcgctata
    15901 tcctcatgaa caatgaaggt ttgacgctgg atgtggcgca tgacaatgtc acaaaagttg
    15961 caacaggtga ggctggtaat gtgacggacc gctggattct ccacaatctc aacgaaacca
    16021 ttgcaaaagt tactgaaaac tttgataagt tcgagtttgg tgtggctggt catatccttt
    16081 acaacttcat ctgggaagaa tttgccaact ggtatgttga gttgaccaag gaagttctat
    16141 acagcgacaa tgaagacgat aaggtcatta ctcgatctgt tctcctctat acgctggaca
    16201 agatccttcg tctccttcac ccaatcatgc cattcgtgac agaggaaatc tttggacaaa
    16261 tctcagaagg ttctatcgtg acagctgcat acccaactgt taatctagcc tttgaagacc
    16321 ttgcggctca cactggtgtg gaaagtctca aagacttgat tcgtgctgtt cggaatgcgc
    16381 gtgcggaagt aaatgtagca ccaagcaagc ctatcaccat ccttgttaag acaagcgata
    16441 gcgacttgga agccttcttt aacagcaatg tcaactatat caaacgcttt acaaatccag
    16501 aacacttgga aatcgcatca accatccctg cacctgaact ggctatgtca agtgtcatca
    16561 caggagcaga aatctacctg ccactggccg atctcctcaa tgttgaagaa gaactggccc
    16621 gtctcgacaa ggaacttgct aaatggcaaa aagaactgga catggttggt aagaaactct
    16681 ctaacgaacg cttcgtagcc aacgccaaac cagaagtcgt ccaaaaagaa tgcgacaaac
    16741 aagctgacta ccaagctaaa tatgacgcga ccgtcgcacg tattgatgag atgaagaagt
    16801 tggtgaaatg atgttaaaaa cctaacccta tcgggttggg ttttacattt gataatgtta
    16861 taataaaaag gagaaacttt aggagaatac tatggggaaa attgttgcta ttgatttatt
    16921 taacggtgca ggtggcacta caagtggcct taaaaaatca ggcatagatg ttcaagttgc
    16981 tgttgaaatt gattccgtag ctgttaaaac ttataagtta aataatcctg aggtgtctgt
    17041 aatagatatg gagtaaataa aacttcaatt ttacaagctt tatatggttc tccagccaat
    17101 aaaagtgtgt ctgaatattg gtttagtaca aatgttgatc aaatagatga agtggatggg
    17161 aaacaatgtt taatctattc atattataac attaaagctt ctagaaatgt agaagttctt
    17221 aaaggccgtt ctggaacaaa aaaaggatta gattattggg aaccatatgc tcctcaaaaa
    17281 caatatgaga tggaaagatt acccaaaaat aaatatattg gaagttcttc gacagacaga
    17341 tgggatggca ttgaaaaaaa tgttgtcttt tgtgattgta aagaatatgt ttctgctttt
    17401 gatttatttt tctatcacta taattttaaa aaaatttcta ctcaacgtag taaacaagat
    17461 tttataagat tacgatctaa gccagttgct gatattttaa agaataatac aagtagctat
    17521 acgcgttata aaaaggaaat ggttatagat aatgttaaag ttgatgataa agtttgtgaa
    17581 attattagtg aaataatgga tgaatcttat acagacattc aaattttaac acataagttg
    17641 tattcaaaag gtgatgatat aaaagcttcc aaaactattt ggatgaaaaa atctggaaaa
    17701 gaatattccg aggcttttgc aggaacaggt gaggctagaa ttattttgtt agtaaatgat
    17761 atagtaaatg cgcaatcaaa ttcattaata ttaatagatg aacctgaaat ctcacttcat
    17821 ccgagtgcaa tctataaatt taaagagttt ttacttcaag agtgtttaaa taaaaaacat
    17881 caaattatta tcactacaca ttctacacaa cttataaaag attttcctag agaagccgtg
    17941 aaacttttag tgaaaaacgg agaaaaggta gatgttattg aaaatattga ttatcaggat
    18001 gcattttttg aattaggtga tgtgtatcat tctaggaaga tgatttatgt tgaagataga
    18061 ctagctaaat atattctaga gtttgttatc actcattcag gtagtgagaa tcttaaacag
    18121 aatttagtag tgagatatat tcctggtgga gcaaatcaaa taatttgtaa taatatttta
    18181 aactcatcgt atttagattc cgataaccat tatttttggc ttgatggaga tcaaaacact
    18241 aatgttagtg aatcaaataa tttaatgaac tatcttgaaa atggtgttgt tatatcagat
    18301 aaaattcctg aatcagataa taaaaatctt gatgatatta taaaattgat aacgggatgt
    18361 ccaattaaat ttaatgtttc aggtaataaa gggcaaaaaa ataatattga attaattgcg
    18421 aaacaaagaa gctttataga ttattgggct aaatacgttt cttatcttcc tttcccaacc
    18481 ccagagtttt ttttagctaa tttgtgtaac agcgtagata gggaaggtta tgatttttct
    18541 aaagatggca acggaaaaga atattttaga aaaaaaactc aagtagcgtt aggaatagag
    18601 aatataactt ctgaagatat tttccaggag caaagaagag ctgtttcaaa aatacagcca
    18661 gaatcatcaa tgtttcagtg tattaaagaa aaattagagg cattgttcta attccttaga
    18721 ttaggtacta cataacaaat tttttaaatt caaacccgca ccaaaaacaa cattgcaact
    18781 ttcttctcag gtaatgtgtt gggatttgaa cagaccaacg agttcatagt ggtgtatgcc
    18841 aatgactttg ataagtctac gcgtcagact tattagttca tttatccaga tatatctcga
    18901 taatcatatt atttagtcaa agaacatccc atatgagggc atgaaatgct aagtttagct
    18961 tgctcactat taagatgaag tatatatatt tcatagaacc tattgtgaag gatttttata
    19021 attttcgcca attccatacc tatttcacaa tttaccgatg acacagaaaa acaattgaaa
    19081 ggatatttct atgcaaccaa cttacaacat tgacaatcca aacttgtctt atgaagcgaa
    19141 gcgtgattta tggcggatag gttttggtct gcagaaagtt gacaatctag tgccatcagc
    19201 gtatatggaa tctttggctg agaaacagtc ccggggtgaa ctgacttatg agcaggttta
    19261 tgaggatgca acggcttatc atcataccat tgatgcgagt acagaggagg cagacttggt
    19321 ttctctacgt attgtagaac tattgtctcg aagaggcttt agcttcagtc ctgcgatctt
    19381 acttgctatt cataaggagt tgtttcaaga tatatttgaa ccctcgattc cggtaggtca
    19441 atttcgtcag actaatatca caaagaatga acctgttttg aatggtgaaa gtgttgtgta
    19501 ctctgattac tccatgattc aaatgacctt ggattatgat tttaatcagg aaaaacaagt
    19561 tgcatatgcg acactaaccc aggcggatat ggttaaaaaa atccagcatt ttatttcagg
    19621 aatctggcag attcatccat ttcgcgaagg aaacactcgg acggtaacgg tatttttgat
    19681 tcagtatctt cgtgagtttg gttttgatat tgataataca ccatttcagc aacattccaa
    19741 gtattttcgt gatgccttag tgttagataa tgcaaagatt ttacagcgac gtcctgagtt
    19801 tttaacagct ttttttgaaa atctcttgct cggtggtcaa aatgatttgt cttcagaaaa
    19861 aatgtatcta gatttagacc tcgatctttc ataatcctaa tactgagtaa acattgaatt
    19921 ttaggaaaaa atgaagtaaa tattctcaca agaaaacgta tatcatcaaa gtttggctct
    19981 ttgtcaattg tagtgggttg aagaaaagct aagttcgaga aaggacaaat ttcgtccttt
    20041 cttttttgat gttcagagcg ataaaaatcc g
//
LOCUS       DBDESG010000047        19310 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.047, whole genome shotgun sequence.
ACCESSION   DBDESG010000047 DBDESG010000000
VERSION     DBDESG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.047"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..479
                     /locus_tag="ACKB38_001580"
     CDS             <1..479
                     /locus_tag="ACKB38_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HGL7980327.1"
                     /translation="EPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPN
                     EPVEVVIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIV
                     GEEIGLDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            460..1266
                     /locus_tag="ACKB38_001581"
     CDS             460..1266
                     /locus_tag="ACKB38_001581"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980328.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVALLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            1263..2036
                     /locus_tag="ACKB38_001582"
     CDS             1263..2036
                     /locus_tag="ACKB38_001582"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980329.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            2033..3103
                     /locus_tag="ACKB38_001583"
     CDS             2033..3103
                     /locus_tag="ACKB38_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7980330.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            3356..3595
                     /locus_tag="ACKB38_001584"
     CDS             3356..3595
                     /locus_tag="ACKB38_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980331.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            3870..4355
                     /locus_tag="ACKB38_001585"
     CDS             3870..4355
                     /locus_tag="ACKB38_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGL7980332.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            4377..6995
                     /gene="alaS"
                     /locus_tag="ACKB38_001586"
     CDS             4377..6995
                     /gene="alaS"
                     /locus_tag="ACKB38_001586"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGL7980333.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(7085..8539)
                     /locus_tag="ACKB38_001587"
     CDS             complement(7085..8539)
                     /locus_tag="ACKB38_001587"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGL7980334.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            8718..9467
                     /locus_tag="ACKB38_001588"
     CDS             8718..9467
                     /locus_tag="ACKB38_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980335.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            9448..11082
                     /locus_tag="ACKB38_001589"
     CDS             9448..11082
                     /locus_tag="ACKB38_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980336.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            11154..11414
                     /locus_tag="ACKB38_001590"
     CDS             11154..11414
                     /locus_tag="ACKB38_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980337.1"
                     /translation="MIKIGNQKYIGRNVLNKKYNIVLFLLFIVYLFGYFSISKTLIPI
                     MCVFQVFLIEHIFRVRNRMMQIGEIIIIVASIILFIDSILSL"
     gene            11769..12932
                     /locus_tag="ACKB38_001591"
     CDS             11769..12932
                     /locus_tag="ACKB38_001591"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGL7980338.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFNVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASHTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            12929..13606
                     /gene="aroD"
                     /locus_tag="ACKB38_001592"
     CDS             12929..13606
                     /gene="aroD"
                     /locus_tag="ACKB38_001592"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGL7980339.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            13596..14450
                     /locus_tag="ACKB38_001593"
     CDS             13596..14450
                     /locus_tag="ACKB38_001593"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGL7980340.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            14469..15536
                     /gene="aroB"
                     /locus_tag="ACKB38_001594"
     CDS             14469..15536
                     /gene="aroB"
                     /locus_tag="ACKB38_001594"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGL7980341.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            15546..16712
                     /gene="aroC"
                     /locus_tag="ACKB38_001595"
     CDS             15546..16712
                     /gene="aroC"
                     /locus_tag="ACKB38_001595"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGL7980342.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            16722..17825
                     /locus_tag="ACKB38_001596"
     CDS             16722..17825
                     /locus_tag="ACKB38_001596"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGL7980343.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            17836..18174
                     /locus_tag="ACKB38_001597"
     CDS             17836..18174
                     /locus_tag="ACKB38_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGL7980344.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            18266..>19310
                     /gene="aroA"
                     /locus_tag="ACKB38_001598"
     CDS             18266..>19310
                     /gene="aroA"
                     /locus_tag="ACKB38_001598"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGL7980345.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVA"
BASE COUNT         5401 a         3778 c         4397 g         5734 t
ORIGIN      
        1 atgagccaat caaccacttt gttgccacct ttatcgggga gtcaaacatc ttgccaggta
       61 ccatgattga ggactacttg gtcgaattta acggcaaacg ctttgaagcg gttgatggtg
      121 ggatgaagcc aaatgaacct gttgaggtcg ttattcgtcc agaggacttg cgcattaccc
      181 ttcctgaaga aggcaagctc caagttaagg tcgataccca gcttttccgt ggagttcatt
      241 atgaaattat cgcctatgac gaacttggaa atgaatggat gatccactca acccgtaagg
      301 ctatcgtggg tgaggaaatc ggtctggact ttgaaccaga agacatccac atcatgcgtc
      361 tcaatgaaac cgaagaagag ttcgatgctc gtattgagga gtacgtagaa atcgaagagc
      421 aagaagcagg tttgatcaat gcaatcgagg aggaaagaga tgaagaaaac aagctctaaa
      481 ctctttgtag taccctacat gctttggatt gcgctctttg tattggcacc cttggtcttg
      541 attttcggtc aatccttttt caacatcgaa ggccagttca gtttagaaaa ttacaaatct
      601 tactttgcgt cacaaaactt gacctatctt aaaatgagtt tcaactcagt gctttatgca
      661 ggcattgtga cctttgtggc actgcttatc agttatccga cggccctctt tttgacccgt
      721 ctcaaacacc gtcaactctg gctcatgctg attatccttc ctacctggat caatttgctc
      781 cttaaagcct atgcttttat cgggattttt ggtcaaaatg gctctattaa ccaattcttg
      841 gaatttatcg gaattggttc acaacagttg ctttttaccg atttttcctt tatctttgtc
      901 gcaagctaca tcgagctccc ctttatgatt ttgccgattt tcaatgtctt agacgatatg
      961 gataataatc tcatcaatgc tagttatgac cttggtgcaa ctaagtggga gaccttccgt
     1021 catgtcatct tccctctatc tatgaacggt gtgcgaagtg gggttcagtc ggtctttatc
     1081 ccaagtttga gtctcttcat gctgacccgt ttgattggtg ggaaccgcgt tatcaccttg
     1141 gggacggcta ttgagcagaa ttttctaacc aatgacaact atggtatggg ttcaaccatc
     1201 ggtgtgattc tcatcctgac catgttcatc accatgtggg tgactaagga aaggagagaa
     1261 cgatgaaaaa atttgccaac ctttatctgg gactggtctt tctggtcctc tacctgccta
     1321 tcttttactt gattggctat gcctttaatg ctggtgatga tatgaatagc tttacaggtt
     1381 ttagctggac tcactttgaa accatgtttg gagatgggag actcatgctg attttggctc
     1441 agacattttt cttggccttc ctatcagcct tgatagcgac cattatcggg acttttggtg
     1501 ccatttacat ctaccagtct cgtaagaaat accaagaagc ctttctatca ctcaataata
     1561 tcctcatggt tgcgcctgac gttatgattg gtgctagctt cttgattctc tttacccaac
     1621 tcaagttttc acttggcttt ttgaccgttc tatctagtca cgtggccttc tccattccta
     1681 tcgtggtctt gatggtcttg cctcgactca aggaaatgaa tggcgacatg attcatgcgg
     1741 cctatgactt gggagctagt caatttcaga tgttcaagga aatcatgctt ccttacctga
     1801 ctccgtctat cattactggt tatttcatgg ccttcaccta ttcgttagat gactttgccg
     1861 tgaccttctt tgtaacagga aatggctttt caaccctatc agtcgagatt tactctcgtg
     1921 ctcgcaaggg gatttcctta gaaatcaatg ccctgtctgc tctagtcttt ctctttagta
     1981 ttatcctagt tgtaggttat tactttatct ctcgtgagaa ggaggagcaa gcatgaaaaa
     2041 aatctattca tttttagcag gaattgcagc gattatcctt gtcttgtggg gaattgcgac
     2101 tcatttagat agtaaaatca atagtcgaga tagtcaaaaa ttggttatct ataactgggg
     2161 agactatatc gatcctgaac tcttgactca gtttacagaa gaaacaggaa ttcaagttca
     2221 gtacgagact tttgactcca acgaagccat gtacactaag ataaagcagg gtggaacgac
     2281 ctacgatatt gccattccaa gtgaatacat gattaacaag atgaaggacg aagacctctt
     2341 ggttccgctt gattattcaa aaattgaagg aatcgaaaat atcggaccag agtttctcaa
     2401 ccagtccttt gacccaggta ataaattctc catcccttac ttctggggaa ccttaggaat
     2461 tgtctacaac gaaaccatgg tagatgaagc gcctgagcat tgggatgacc tttggaagcc
     2521 ggagtataag aattctatca tgctctttga tggggcgcgt gaggtgctgg gactaggact
     2581 caattccctc ggctacagcc tcaactccaa ggatctgcag cagttggaag agacagtgga
     2641 taagctctac aaactgactc caaatatcaa ggctatcgtt gcggacgaga tgaagggcta
     2701 tatgattcag aataatgttg caatcggcgt gaccttctct ggtgaagcca gccaaatgtt
     2761 agaaaaaaat gaaaatctac gttatgtggt accgacagag gccagcaatc tttggtttga
     2821 caatatggtc attcccaaaa cagttaaaaa ccaaaactca gcctatgcct ttatcaactt
     2881 tatgttgaaa cctgaaaatg ctctccaaaa tgcggagtat gtcggctatt caacaccaaa
     2941 cctaccagcg aaggaattgc tcccagagga aacaaaggaa gataaggcct tctatcccga
     3001 tgttgaaacc atgaaacacc tagaagttta tgagaaattt gaccataaat ggacagggaa
     3061 atatagcgac ctcttcctac agtttaaaat gtatcggaag taggagataa acgtaagaag
     3121 acgaatcagt caagctggtt cgtttttaag gaatagaagt cagtttttag agccctatct
     3181 ttttctattc tagttgttta tttgtttatt tgcttgccta gtagtatact aagcctatac
     3241 tcttcaaacc acgtcagcgt tgccttgccg tactcaagta cagcttgagg ctagcttcct
     3301 agtttgcttt ttgattttta ttgagtatta accttacaga aagcggcaac aaataatgaa
     3361 aatgtctact tttttcaaaa aaagtttctg gccaaccttt atgattgtta accaaactgc
     3421 tatactattc catttaaagg atggcttgga tcgacaatac ctaacaactg agtccatata
     3481 ttgggtgata ggcaccttta tatttggaaa tattctagta gctgtgttta gtaatatgaa
     3541 aatttgggat aaaaagaaaa atggcagtaa gaaaaaatat atcctgaaaa agtaatcaga
     3601 gatgtaggga attggtctaa ttcaacggag atcatttcca gttgacgcaa gcttaaaaaa
     3661 acgttataat aagaaagttg agaattgatt ttcacttgtc ttagtccaga gaaatggcgg
     3721 tgctgcgagc catctaagct aggaagtcat gctactcaat catacaattg cataagaaca
     3781 agagaatgac aagttcattg aatgaaggtg gtaccgcggt ttttcgccct tcgtgatatg
     3841 agcttgtctt ttgatttttg gaggtgttga tgaaaacatt tcttgtcaaa caaaagtttc
     3901 gtcttggagg cgaacgcttc gctatcaagg atgacagggg agaaattgcc tatcaggtgg
     3961 agggatcatt ttttaagatt cctaaaacct ttaccatcta tgatgcggct ggtgaacaag
     4021 tcagtgagat tagtaaagaa atcttgacct tgcttcctcg ttttgaaatt cagcttcggg
     4081 atggctcgag ttttgtcatt cgtaagaagt tgaccttctg gcgagataag tatgagtttg
     4141 ataatctagg tcttcgtatc gagggcaata tctgggattt gaatttcaaa ttgctggatg
     4201 atcgcgatca gttgattgcg gaaattaaga aggaactctt ccatctgact tctacctata
     4261 ccgtaacggt tcttgaagac gcttatgcag acctagtcat ttccctctgt gtcgcgattg
     4321 actatgtgga aatgctggaa agccaatcac attaaacaag tgaataagga gacaatatga
     4381 aacaactatc tagtgcacaa gtacgccaaa tgtggcttga tttctgggcg accaaaggtc
     4441 actcagtaga accatcagta agtttggttc ctgtaaatga cccaactctt ttgtggatca
     4501 actctggggt agcaaccctt aagaaatact ttgacgggac cattatccct gaaaatccac
     4561 gtattaccaa tgcccaaaag gctatccgta ccaacgacat cgaaaacgta gggaagaccg
     4621 cgcgtcacca taccatgttt gaaatgctgg ggaacttctc tatcggggat tacttccgtg
     4681 acgaagctat cacttgggct tatgagcttt tgacaagccc tgaatggttt gatttccctg
     4741 ctgaaaaact ttacatgacc tactatccag acgataaaga ttcttacaac cgctggattg
     4801 aagtgggagt ggatccaagt cacttgattc caattgagga caacttctgg gaaatcggtg
     4861 cgggaccttc tggaccagat acagaaatct tctttgaccg tggggaagcc tttgacccag
     4921 aaaatatcgg tcttcgcctg cttgcagaag atattgaaaa cgaccgttat attgaaatct
     4981 ggaacatcgt tttgtcacaa tttaacgcag accctgctgt tcctcgtagc gaatacaagg
     5041 aattgccaca taagaacatt gatacgggcg ctggtttgga gcgtttggtg gccgttatcc
     5101 aaggggctaa gaccaacttt gaaacggacc tcttcatgcc gattatccgt gaagtcgaga
     5161 aattgtctgg taaggtttat gaccaagatg gcgacaacat gagcttcaag gttatcgcag
     5221 accacattcg ttctctttca tttgctatcg gtgatggtgc ccttccagga aatgaaggtc
     5281 gtggttatgt tcttcgtcgt cttctccgtc gtgcttctat gcatggtcaa aaattgggta
     5341 tcaacgagcc tttcctttac aaattggttc caactgttgg aaaaatcatg gaaagctact
     5401 acccagaagt gcttgagaaa cgtgacttta ttgagaaaat cgttaagagc gaagaagaat
     5461 catttgcccg tacccttcac tcaggtcaac actttgccca aggcattgta gcagacttga
     5521 aagaaaaagg tcaatctgtt atcgctggtt cagatgtctt caaactctat gatacttatg
     5581 ggttcccagt tgaattgact gaagaaatcg ctgaagaagc tggtatgact gtagaccgtg
     5641 aaggttttga agcagccatg aaagaacagc aagaacgcgc gcgtgcgtca gctgtcaagg
     5701 gtggctcaat gggtatgcaa aatgaaactc ttcaaaacat cactgtagaa agtgtcttca
     5761 actacaatgc tagccaattg tcttctaaat tggtagctat cgttgctgac aatgcagaag
     5821 taggagctgt ttcagaagga actgcctctc ttatctttgc ggaaacgtca ttttatgctg
     5881 aaatgggtgg acaggtagct gactacggac aaatcttgga tgagtcaggt aaggttgtgg
     5941 ctactgtgac caatgttcag aaagccccaa atggtcaagc ccttcataca gttgaagtcc
     6001 ttgcaccgct tgccttgaac caagaatata ccttggcaat tgatagcaat cgccgtcacc
     6061 gtgtcatgaa aaaccacact gcgactcatt tgcttcacgc tgcccttcac aatatccttg
     6121 gaaaccacgc aacacaggca ggatctctta acgaagttga attccttcgc tttgacttta
     6181 cccacttcca agcagtaact gctgaggaac ttcgtgcgat tgaacagcaa gttaacgaga
     6241 aaatttggga agcacttgaa gtgaagacag ttgaaacgga tattgacact gctaaagaaa
     6301 tgggagctat ggccctcttt ggtgagaaat acggcaagga agttcgtgtc gtgactatcg
     6361 gtgactactc tattgaactt tgtggtggta ctcatgttga caacacttct gagattggtc
     6421 tcttcaaaat tgtcaaagaa gaaggaatcg gttcaggaac tcgccgtatc ttggcagtga
     6481 ctggtaagga agcctttgaa gcctatcgtg aacaagagga tgctcttaaa gctatcgcag
     6541 caaccttgaa agcacctcaa gtcaaggaag tacctcacaa ggtagaagga cttcaagaac
     6601 aacttcgtca acttcaaaaa gaaaatgctg agttgaaaga aaaagccgca gctgcagccg
     6661 caggcgatat cttcaaagat gttaaggaag tcaacggtca tcgttacatt gctagtcaag
     6721 tgtctgtatc cgatgccggt gcccttcgta cttttgcaga taactggaaa caaaaagact
     6781 actctgatct tcttgtccta gttgccgcta tcggtgacaa agtcaatgtc cttgtagcaa
     6841 gcaagacaaa agaccttcat gcaggaaacc ttgtcaaaga attagcacca atcatcgatg
     6901 gacgtggtgg tggtaaacca gatatggcca tggcaggagg aagcaaccaa cctaagatcc
     6961 aagaattgtt ggatgcagta gcaggtaaat tgtaatcaag taaagatcta tccatttggg
     7021 taggtctttt tgtgaataca aaaaagccaa atccgcttgg acctagcttg gttattatga
     7081 gctattagat ggtattgact gcccagacac ttacggatct agctgagaca gggaattgtc
     7141 cataaccttc ctcatcaatt gtaacttgac cttggtggtt accaagtaaa tctacaaagg
     7201 tttgattagt ccattcttga ccgacaaaca ttgacttgct gttttcttgg tcatttgaga
     7261 taaggactgc gattggggat tgattttcag cacctgaacg tacccaaccg atacagttag
     7321 catggtcaaa gtagtcattt tgttctccat aggccaaatc ttttcggatg gctaggaggc
     7381 ggtcaaggat ttctttgaaa tcttcttgag catactgccc tgaaatccca tagtagtctc
     7441 cgtaaaagac acatggaagg ccgtcttggc gtaacaaaat gagggcatag gctgctggct
     7501 tgaaccattc ttcaacggta gactcaaggg cttgtcctcg ttgggtatcg tggttgtcga
     7561 caaaagtcac agccttgtca ggcttgagtt caaccaggct atctgtgaaa atgccacgaa
     7621 ggtcatagtt tgcgccagct tgactggctt caaagagatt ctggtggaga cgaacatcga
     7681 caaggtcaaa gtgttcttcc gttttttcga gatagtccag attggcttcc ttgtctgggt
     7741 tccaaaattc accaaaaaca tagaaatcgt caccgtattt ttccttcata tcgcggatga
     7801 agttgcgcat aaagaaagag tcaatatgct taacggcatc caaacggaaa ccagctacac
     7861 cagtcgtttc catgaaccaa tcagcccagt catagatgtt ttggatgact tcaggatgtt
     7921 taaagtctag gtcggcatac atgaggtagt cgtagtttcc gttttcgtta tcgaccaatt
     7981 cctcgttggc ccagcccttg ttgtcccctt ggatcagata aatcccagat ttactgcgtt
     8041 tggcatcgta gtctgtaccg gtgaagtggt accaatgcca gtggaagcca ttataggtat
     8101 cttggcgacc atcgaaggta aaactagtcc agccattgat ggtgaagggt tctccaagtt
     8161 caactgtacg gtctacagga tcaacttcga taacctgaaa ggcttccctg tgatcggcag
     8221 cagccttgtg gttgagaact acatcggcca taggttgaat tccctgtgct ttaagggctt
     8281 gaatggcttg aagatagtct tctttgaaac catacttggt gcggacagtc cctttttggt
     8341 tgaactctcc taagtcaaat aagtcataga ccccatagcc gacatctttt tcgttggttg
     8401 ccttgaaggc tggtggcatc cagacgtgac tgatccccag atgagctagg tgtggagcat
     8461 tttcagccag acgcgtccag tgttgaccgt cgtggggcag ataccattca aagtattgca
     8521 tgagtgtttg attttgcata atgtttcctc ttacttgtca atcttgttct ttattctatc
     8581 ataaagtatc tattagcgca aacgtttgcg taagatagaa ggaaactatt ttaactactt
     8641 aaaataaagt attgattttt ttgtatcaaa gtgctagtat agcgatatat aatacagata
     8701 aagataggag ttgtcccatg attgaatttc aaaatgttag taagttgtat ggtgataaag
     8761 aggccttgag taatctcaat ttgcagattg aaaatggaga gattatgggc ttgattggtc
     8821 ataatggggc tggaaaatcg accactataa aatccctagt cagtatcatt tcacccagca
     8881 gtggtcgtat tttggtagac ggtcaggagt tatcggaaaa tcgcttggct attaaacgaa
     8941 agattggcta cgtagcagac tcgcctgact tatttttacg cttaacggcc aatgaatttt
     9001 gggaattgat cgcctcatcc tatgatctga gtagatctga cttggaggct agtctagcta
     9061 ggctattgaa cgtttttgat tttgctgaaa atcgctatca ggttattgaa actctttctc
     9121 acggaatgcg tcagaaagtc tttgtcatcg gagcactctt gtctgatccc gatatttggg
     9181 tcttggatga acccttgact ggtttggatc cccaggctgc ctttgatttg aaacagatga
     9241 tgaaggaaca tgcacaaaaa gggaagacag tcttgttttc aactcatgtc ctagaggtgg
     9301 cagagcaagt ctgtgatcgg attgccattt tgaaaaaggg gcatttgatt tattgtggta
     9361 gtgtagagga cttgagaaaa gattacccag accagtcttt ggaaagtatc taccttagtc
     9421 ttgctggtag aaaagaggag gttgcggatg cgtctcaagg tcattaaaaa attagttgat
     9481 atcaatatcc tttattcatc tcaagaagct aatctggcta atctacgaaa gaagcaggct
     9541 aagaatcctg ggaaaaaagt aaatgtttcc gctagagtct taagttctta cattttttcc
     9601 agtctcttga tgatcatctg ttttagtaat atagccattc attttccttt tgaggaaata
     9661 cctatttatt ttagctcgat gattgctatt ttactagtga ttgccttttc aacttcttta
     9721 acggcctttt acaatgtctt ttatgagagt aaggatttgg tatcgtatag gccctatgcc
     9781 tttaaagaat cagagattat aattgctaaa ggactgtctg tccttttgcc agctctaact
     9841 ggaattgtac caatcctagc ttattttctg gtcctctaca ttaggctagc tccttctcta
     9901 tggctgggtt tgcctttgat gctactgtcc ttgaccttat tatttgtctc tgtggctcta
     9961 gtgatggtag tggcagtgca tttcttggct caaactaggg tcttcagaaa gtatcagtct
    10021 attttttcga atgtgatgat tgggatagga gttctcatac ctttaatatt tatcttcttt
    10081 cttcagtcga cttttggaag tattgttgac aaagttagag acattccatt tctcctttat
    10141 cctcttcata tcttttacaa aatagcagtg gagccttttt cgacagaagc cttagtgggt
    10201 ctgctcgctt ggataggact aactctcttc ctgctttatc tgaccaaaaa gaaggtcctt
    10261 cctcgttttt atgacgtgat cctgcttaat agtgaggaga aggtcaaaaa agaacgtcgc
    10321 agcaaggaga gaatttcaac cactaaaaag ggattttttc gcatggtttt acgctaccac
    10381 ctcaccctct tgggacaggg gactggcgtg attacagtgc tttttacaag tgctttcctt
    10441 ccttatctca tgatgatcgg tctgatttcc aaaatccgag attctcagat agttccagac
    10501 attcatcctc catactggtt acccttgttt tttatagctc tctttatagc agttgtcaat
    10561 aacaatatca ccagcctgca ttcaattgcc ttgtccttgg agagggaaaa tgttgatttt
    10621 cttaagagtt taccctttga ctttgctcgt tatgtgaaag tgaaattttg gattatatat
    10681 gctgtccagt cctttttacc agttctgact ttgcttggtc tttctctata tctaggcttg
    10741 cccatcattt cgatgattta ccttcttgta gtctggatca ttgccagtgt catcctttct
    10801 tgccaccatt actttaagga cgttaaaaat ctgtcaacca attggagtag cattacggac
    10861 ctggtgaatc gttcaaatgg catagtcgcc atagttttat tgtttattta tagtgcaatc
    10921 ctgatggccc ttgtaattgg gagcatattc ttggttcagt ctctctcccc tatccttgcc
    10981 atcagcttgg gagtaggagc tcttatcgtc ttgcttgctc ttgctatttt tggctatcat
    11041 tattacctgt cacgcatatt ggcagaaata gaaaaaagat gaactggttg gtcgcttgct
    11101 tgataaattc aattcgacaa acgcttttat agtttgttag aatgtagttg taaatgatta
    11161 agattggtaa tcaaaaatat atagggagga atgttttgaa taagaaatat aacatagttc
    11221 tatttttgtt gtttatagtt tatttatttg gatatttttc aatttcaaaa acgttaattc
    11281 ctataatgtg tgtgttccaa gtatttttga tagaacatat atttagagtt cgaaatagaa
    11341 tgatgcagat aggtgaaatt ataattattg ttgcttctat aatattattt attgatagta
    11401 tattgagttt gtaacaaaag gcttctctat aaaatcaaat tttccagata cctactgaag
    11461 ctgatttatt tctttatctt tcttctacta cctttgtcac taaatttgac agacacatat
    11521 tgctgataca acctctctac ctataatctc acagttaacc tgaggctagt ttcatagttt
    11581 gctatttgat tttcgttgat aaaaagaaat gctcctccaa gacggaagag cattttttac
    11641 tgtgattctt acacatttac ttcctagttt tgcaggaagt ctagtatacc attttttaaa
    11701 agaagagtaa aaaagcaagg gtagtttctt ttttgagaat tgtctaaaag tttgatataa
    11761 tggtagttat gaatagaata agagtcagca aaagggttga aaagaagctt gctaaggggc
    11821 tagttttact agaagccagt gatcttgaga atgtcaatct taaggatcag gaagtagagg
    11881 tgcagggtca ggaaggaaac tttcttggga ctgcctacct ttctcagcaa aacaagggct
    11941 tgggctggtt tatcagcaaa gacaaggtgg ccttcaatca agctttcttt gaaacgttgt
    12001 ttagaaaagc caaagaaaag agaaacgcct actatcaaga tgatttgaca actgcctttc
    12061 gtctctttaa tcaagaggga gatggctttg ggggtctgac agtggacctt tatggcgact
    12121 acgccgtctt ttcttggtat aactcttatg tttatcagat tcgtcagact atatcagaag
    12181 cctttagaca ggttttccct gaggttttag gagcttatga gaaaatccgc tttaagggtt
    12241 tggactatga atctgcccat gtttatggtc aagaagcacc tgactttttc aatgttttag
    12301 aaaatggtgt cctgtatcaa gtctttatga atgatggctt gatgacagga attttcctag
    12361 accagcatga ggttcgcggt agtttagttg acggcttggc tatgggtaaa tccttactca
    12421 atatgttttc ctacacagcg gctttttcag tagctgcggc catgggagga gctagccata
    12481 caacttctgt tgatctagcc aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa
    12541 atgggctcag cacagacgag catcgtttta tagtcatgga tgtctttgag tatttcaaat
    12601 atgccaaacg caaagacttg acctacgatg tgattgtcct agatccgcct agctttgctc
    12661 ggaataaaaa acaaactttc tctgtggcca aggattatca caagttgatt tcccagagtc
    12721 ttgagatttt aaatccggga gggattatca ttgccagtac caatgctgcc aatgtttccc
    12781 gtcagaaatt tacagaacaa attgataaag gttttgcagg aagaagttac cagattttaa
    12841 acaaatatgg tcttccagca gattttgcct ataataaaaa agatgaaagt agtaattacc
    12901 tcaaggtgat tagtatgaag gttagtaaat gaaattaatc gtttcagtaa tgccaagaag
    12961 tttagaggag gctcaggctc tggatgccac gaggtacctg gatgccgaca tcattgaatg
    13021 gcgtgccgac tatctgccta aagaagcgat tttgcaggta gctccagcca tttttgaaaa
    13081 attcgcaggc cgtgagttgg ttttcacgct acgaactcgc tccgaaggtg gagaaatcga
    13141 cctttctcca gaagagtata tccatctaat caaggaagtt gcgcaactct atcaaccaga
    13201 ctatattgat tttgagtact atagctacaa ggatgttttt gaggaaatgc tggacttccc
    13261 aaatcttgtt ttgagttacc ataatttcca agaaacacct gagaacatga tggaaatctt
    13321 gtcagagttg acgatcctaa atccaaaact tgttaaggtt gcggtgatgg ctcacacgga
    13381 gcaggatgtc ttagacttga tgaactatac acgaggcttt aaaaccctca atcctgaaca
    13441 ggaatatgtg accatttcta tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt
    13501 gactggttcg agttggtctt ttgccagtct ggatgaggtt agtgcccctg ggcagatttc
    13561 tctagctagc atgaaaaaaa tcagggagat tttggatgaa gcttgatggc tatacacgtt
    13621 tagctgcagt tgttgccaat cctattaagc attctatttc tcccttcatc cacaatagag
    13681 cctttgaggc gacagctacc aacggtgctt atgtggcttg ggagattgaa gcgagtgact
    13741 tggtagaaac agtggccaat attcgtcgct accagatgtt tggcatcaat ctgtccatgc
    13801 cctataagga gcaggtgatt ccttatttgg ataagctgag cgatgaagcg cgcttgattg
    13861 gtgcggttaa tacggttgtc aatgagaatg gcaatttaat tggatataat acagatggca
    13921 agggattttt taagtgcttg ccttctttta caatttcagg taaaaagatg accctgctgg
    13981 gtgcaggtgg tgcggctaaa tcaatcttgg cacaggctat tttggatggc gtcagtcaga
    14041 tttcggtctt tgttcgttcc gtttctatgg aaaaaacaag accttaccta gacaagttac
    14101 aggagcagac aggttttaaa gtggatttgt gtgctttaga atatgtttct gaactgcaag
    14161 caaggattgc cgagtcggat ttgctagtta atgccaccag tgtgggcatg gatggccaat
    14221 cctctcctgt tcctgaaaac atagtcttac cagaaactct tttagttgca gatatcatat
    14281 accaaccctt tgaaacaccg tttttgaaat gggctagaag tcagggcaat ccagcagtca
    14341 atggtctggg aatgttactc tatcaagctg cagaagcttt tcaactgtgg acaggcaagg
    14401 aaatgccgac agaagagatt tggcagtctt taacagaaaa ataccaataa agaaagggag
    14461 actctcctat gaaaatcaga atcgatattc ctcaccatcc ttatgatatt cagattgaaa
    14521 aaggttgtat ggcccaggct ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag
    14581 tcattgtgac agataaccat gtagcctctc tctatgcaga gaaggtcaag ctcagcctag
    14641 aagatgctgg ttttcaggta gctgtttttg atttcttaga aggtgaagaa agaaagaatt
    14701 taactactgt tcagaaagtc tatgaatttc tagtcaagca aggtctgact cgtagcgatg
    14761 gaatcgttgc tcttggtggt ggcgttgttg gggacctggc tggatttgta gcctctacct
    14821 atatgcgggg tattcacttt gttcagattc cgactagttt gacagctcag gttgattctt
    14881 ctatcggtgg aaagacaggc gttaatacac catttgctaa aaatatggtg gggacctttg
    14941 cccaaccaga tggggttctg attgatccac ttgttcttga aaccctcgga aaaagagagt
    15001 tgattgaagg gatgggtgaa gttatcaagt atggcttgat tgaggatcca gaactgtggg
    15061 ctctcttgac gggactgaat ggttctgttg agagtatttt ggaacatgca gagaccttga
    15121 ttgaacattc ttgtcaggtg aagcgcaaga tggtggttga agatgagttg gacaatggta
    15181 ttcgtcttta cctcaatttt ggccacacta ttggccatgc catcgaagcg actgccggtt
    15241 atggcaaggt catgcatgga gaggctgttg ccatgggaat ggtacagatt tccaaggttg
    15301 ctgaggaaaa aggcctcatg ccagctggca ttacccaatc tatcacagag atgtgtcaga
    15361 aattcggttt gcctgttgac tatgaaaatt gggaagttga caagctttat caggctctta
    15421 ctcatgacaa gaaagcgcgt ggtaacacct tgaaattggt cttggtgcca gagcttggtt
    15481 cagcgaccat tcacccagtt tctctggaag agatgaaaga ctacttggta aaataaggag
    15541 agtgtatgag atatttaact gcaggagaat cacacggccc ccgtctaaca gctattattg
    15601 agggaattcc agctggactt ccattgacag ctgaggatat caatgaggac cttagacgcc
    15661 gtcagggtgg ctacggtcgt ggtggtcgta tgaagattga gaatgaccag gttgtcttta
    15721 cttcgggcgt tcgccacggg aagacgacag gggcgcctat tactatggat gtcatcaata
    15781 aggaccacca gaaatggctg gacatcatgt ctgcggagga cattgaagac cgccttaaaa
    15841 gcaagcggaa aattactcat cctcgcccag gtcatgccga tttggttggg gggattaagt
    15901 accgttttga tgatttgcga aattctttgg agcgttcatc agctcgtgaa accaccatgc
    15961 gggtggcagt tggtgcagta gccaaacgcc tcttggctga gctggatatg gagattgcca
    16021 accatgtcgt ggtctttggt ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg
    16081 aaattaagca aagagctgcc cagtctgaag tttctattgt caaccaagaa cgagaacagg
    16141 aaatcaagga ctatattgac caaatcaaac gtgatggtga taccatcggt ggggttgtgg
    16201 agacagtcgt cggaggcgtt ccagttggtc ttggttccta tgtccaatgg gatagaaaat
    16261 tggatgcaag attggctcaa gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg
    16321 gtcttggctt tgaggctggt tatcgtaaag gcagccaagt tatggatgaa attctctggt
    16381 ctaaagaaga cggttatact cgccgtacca ataatctagg tggttttgaa ggtggtatga
    16441 ctaatgggca acccatcgtt gttcgtgggg tcatgaaacc cattcctact ctttataaac
    16501 ctcttatgag tgtggatatc gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg
    16561 atccgactgc tcttccagct gcaggaatgg tcatggaagc agttgtagca acggttctgg
    16621 cgcaagaaat cctcgaaaaa ttctcatcag ataatcttga ggaactaaaa gaagcggtag
    16681 ccaaacaccg agactataca aagaactatt aaggagttcc tatggcaaaa acaatctata
    16741 tcgcaggtct tgggttgatt ggagcctcta tggcacttgg tatcaaacgc gatcatccag
    16801 attatgaaat tttaggttat aatcgtagtc aagcttcgag agatatcgcc ttgaaagaag
    16861 gtatgattga ccgtgcaacg gatgattttg ctagttttgc tcctttggca gatgtcatta
    16921 tcctcagctt gccaatcaaa caaactattg ctttcattaa ggagttggcc aatttggatt
    16981 tgcgagaagg cgttattatt tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg
    17041 cggagcagta tttggctggc aagtctgttc gctttgtcgg ggcccatccc atggctggta
    17101 gtcacaagac aggggctgct tcggcagatg tcaatctttt tgaaaatgcc tattatatct
    17161 ttacaccttc aagcctgaca agtcaggaca cgcttaagga aatgaaggat ctgctttcag
    17221 gtcttcatgc tcgttttatc gagattgatg ccaaggagca tgatcgtgtc acttctcaga
    17281 ttagccattt tcctcatatt ttggcttcta gtctcatgga gcagactgcg gtctatgctc
    17341 aagagcatga gatggcaagg cgctttgcgg caggtggttt tcgagatatg acccgaattg
    17401 cggaaagcga gccaggaatg tggacctcca ttctcttgtc caatagcgag atcattctgg
    17461 atagaattga ggatttcaag gaacgtttgg aagcgattgg tcaggccatt agtaagggag
    17521 atgaagagca aatttggaac ttttttaacc aagcgcgtga acaacgccag accatggaaa
    17581 tccataagcg tggtggtgtg gatagctctt acgacctcta tgttgacgtt cctgatgaag
    17641 aagatgtcat cctgcggatt ttggaactgc tacgtggaac ttccttggtt aatatccaca
    17701 tcaacgagga aaatcgtgag gatattcacg gaatcctaca aatttcattt aaaaatgctc
    17761 aagacttgga aagagctgag catctcataa cagaaaatac tgactataca gtcgtcatca
    17821 aataaggaga agaacatgtc aaatatttac gatagtgcaa acgaactcag tcgcggtcta
    17881 cgcggattac cagaatacaa ggctgttaaa gcagctaaag atgcgattgc agcagatgct
    17941 gaggcaagca aaatctttac agattatctt gcctttcaag aggaaattca aaaactagca
    18001 cagacaggtc aaatgccaga tgcgtccttc caagctaaga tggaaggctt tggtaaacag
    18061 attcaaggaa atagcctctt gtcagaattc tttaccaagc aacaacaatt ggccatttac
    18121 ctttctgata ttgaaaaaat tgtttttgaa ccagtttcag aattgctgaa ataagaaaag
    18181 aacgatccta aagtcaggaa tttttaagga atttctggct ttttatgata aaataggtaa
    18241 aagtattgca agtatgaggt ccagtatgaa actaaaaaca aacattcgcc atttacatgg
    18301 tagtatccgc gtcccaggtg acaagtctat cagccaccgt tccattatct ttggaagttt
    18361 ggctgagggt gagaccaagg tttatgatat tctgcgaggt gaagacgttc tttcgaccat
    18421 gcaggttttt cgtgaccttg gtgttgaaat tgaggataaa gatggggtta ttaccattca
    18481 aggtgtaggc atggctggct taaaagcgcc acaaaatgcc cttaatatgg gaaattctgg
    18541 cacctcgatt cgcctgattt caggtgtcct tgctggtgca gatttcgaag tagagatgtt
    18601 tggagatgat agtctttcca aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat
    18661 gggcgtcagc atttcagggc aaactgaacg agacttgcct ccccttcgct taaaagggac
    18721 gaaaaaccta agacctattc attatgagtt gccaattgcc tctgcccaag tcaagtcagc
    18781 cttgatgttt gcagccttac aagctaaggg ggagtcagtt attatcgaaa aagagtacac
    18841 ccgtaatcat actgaagata tgttgaaaca atttggtggt catttaagtg tggatggtaa
    18901 gaaaatcaca gtccaggggc cacaaaaatt gacaggacag aaggtggtcg taccaggaga
    18961 tatttccagt gcagcctttt ggttagtcgc aggtttgatt gctccaaatt ctcgtctagt
    19021 gctgcagaat gtggggatca acgaaactcg caccggtatt attgatgtca ttcgtgccat
    19081 gggtggaaaa ttggaaataa ctgaaatcga tccagtcgct aaatctgcta ccttgattgt
    19141 tgagtcttct gacttgaaag gaacagagat tggtggcgct ttgattccac gattgattga
    19201 tgaattgccc attattgccc tgcttgcgac ccaagcccaa ggtgtaacag ttatcaagga
    19261 tgctgaggag ctcaaggtca aggaaacaga ccgtattcag gttgtggcag
//
LOCUS       DBDESG010000048        18697 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.048, whole genome shotgun sequence.
ACCESSION   DBDESG010000048 DBDESG010000000
VERSION     DBDESG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.048"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(365..916)
                     /locus_tag="ACKB38_001599"
     CDS             complement(365..916)
                     /locus_tag="ACKB38_001599"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980346.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1266..2090)
                     /gene="nadE"
                     /locus_tag="ACKB38_001600"
     CDS             complement(1266..2090)
                     /gene="nadE"
                     /locus_tag="ACKB38_001600"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGL7980347.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2087..3547)
                     /locus_tag="ACKB38_001601"
     CDS             complement(2087..3547)
                     /locus_tag="ACKB38_001601"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGL7980348.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3663..4151)
                     /locus_tag="ACKB38_001602"
     CDS             complement(3663..4151)
                     /locus_tag="ACKB38_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGL7980349.1"
                     /translation="MKNRFYYSQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYGRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4141..4350)
                     /locus_tag="ACKB38_001603"
     CDS             complement(4141..4350)
                     /locus_tag="ACKB38_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGL7980350.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4735..5206)
                     /locus_tag="ACKB38_001604"
                     /pseudo
     CDS             complement(4735..5206)
                     /locus_tag="ACKB38_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5231..6109)
                     /locus_tag="ACKB38_001605"
                     /pseudo
     CDS             complement(5231..6109)
                     /locus_tag="ACKB38_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(6444..7172)
                     /locus_tag="ACKB38_001606"
                     /pseudo
     CDS             complement(6444..7172)
                     /locus_tag="ACKB38_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            complement(7418..7729)
                     /locus_tag="ACKB38_001607"
     CDS             complement(7418..7729)
                     /locus_tag="ACKB38_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGL7980351.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LEHHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(7744..9030)
                     /locus_tag="ACKB38_001608"
     CDS             complement(7744..9030)
                     /locus_tag="ACKB38_001608"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGL7980352.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAVTEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            9627..10211
                     /locus_tag="ACKB38_001609"
                     /pseudo
     CDS             9627..10211
                     /locus_tag="ACKB38_001609"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            10208..11398
                     /locus_tag="ACKB38_001610"
     CDS             10208..11398
                     /locus_tag="ACKB38_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGL7980353.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGVDM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(11908..12900)
                     /locus_tag="ACKB38_001611"
     CDS             complement(11908..12900)
                     /locus_tag="ACKB38_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980354.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDNFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            13060..14814
                     /locus_tag="ACKB38_001612"
     CDS             13060..14814
                     /locus_tag="ACKB38_001612"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980355.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            14798..16519
                     /locus_tag="ACKB38_001613"
     CDS             14798..16519
                     /locus_tag="ACKB38_001613"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980356.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLILIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSEKVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            16530..17159
                     /locus_tag="ACKB38_001614"
     CDS             16530..17159
                     /locus_tag="ACKB38_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGL7980357.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            17060..17617
                     /locus_tag="ACKB38_001615"
     CDS             17060..17617
                     /locus_tag="ACKB38_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGL7980358.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            17605..>18697
                     /locus_tag="ACKB38_001616"
     CDS             17605..>18697
                     /locus_tag="ACKB38_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980359.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKI"
BASE COUNT         6352 a         3225 c         3168 g         5952 t
ORIGIN      
        1 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
       61 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
      121 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
      181 tagctccctc ttagttacaa gcttaaagcg attttttaac taacactaaa ccagattaaa
      241 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
      301 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
      361 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
      421 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
      481 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
      541 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
      601 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acaatgctga
      661 tatctcctat tacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
      721 gattagtata ggaagcaacc caattgcaaa tcgagtttcg agtgatttcg acatcaggat
      781 ggggatccca ggtaacatag gtcagattct cagcggatga agcccaattt tgaaacatgg
      841 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
      901 taccgataga tttcatgatg ctacctcact tccttatttc tatactcgta gtaaaaaggc
      961 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agttgctgcc
     1021 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
     1081 gatgaaatcg aactcttcga gttactgaat ctgcctcatt ttcttatttc taaactcggg
     1141 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
     1201 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtcccccgga
     1261 cctttttact cccaaaagtc atcaaatacg gtgatgggta agtggcgttt gtgttggcct
     1321 ttgtgccacc agttttcaat ggtcgcttga gcttctgggc tgattgtttt gccttctagg
     1381 tagtcgtcaa tctctgcgta ggtgactcca agtgcgactt cgtcagctag gcctggttta
     1441 tcttcttcta ggtctgccgt tgggattttt tcataaaggg ctggctctgc gccaagtttc
     1501 tgcaagagct gttttccttg gcgtttattg aggcggtaaa gagggagaat atccgcaccg
     1561 ccgtcaccaa acttggtaaa gaaacctgtg atattttccg cggcgtggtc tgttccaatg
     1621 accgctccgc tatgggaacc agcaagggca tactgagcaa tcatacggca acgtgccttg
     1681 atattcccct tgttgaagtc tgaaacagga ctacctgtcg cttcaactgc agctgtcatg
     1741 gcatcagctg attccttgat attcacaacc aagctgacat ctggctggat gaaggctagg
     1801 gctttttgag catctgcttc atcagcttgc actccgtatg gcaggcggac agcgataaat
     1861 ttgtagctat cgtctcccgt ttcagctcgc agttcttcca tagctaattg cgccaaacgt
     1921 cctgccaagg ttgagtcttg tcccccagaa atccctagta caaaggtttt taggaaggga
     1981 tgttttttca gatatctttt taagaaatca atagaacgac ggatttcttc ctgggcatca
     2041 atcactggtt tgacacccag ctcttggata atcgtttctt gcaaactcat tcttcttctc
     2101 cttcaccaag ggcttccttg cgcatcttat caatcaagtc catcttatct tgccatacat
     2161 cacgcgccaa atccactgga tagtgctgcg gattgagcac acgcttatac tcatcccaca
     2221 acttgtcaaa ttccttacgg gcataatcct gaatgtcagt caaactaggc aagttgtaaa
     2281 ctaatattcc ttctttgaag atatccacca agagaggaac ggcatcaaaa ttacgaaccg
     2341 tcttcttgat gtatgtatag gtcggatgga acatcttgat ttctgtcatg tcgctaatat
     2401 ccacaccatc ataagtgata tagtcgcctt ctgacttgcc tttttcacga ctggtaatgc
     2461 gccacacctg cttcttacct ggcgtagaaa ctttttcagc attattagac agcttaatcg
     2521 tattgcgcat ctgaccagtt tcatcttcga ttgcaacaat cttgtaaacc gccccaagag
     2581 ccggctggtc ataggctgta atcagcttgg tacccacacc ccagacatca atcttggcct
     2641 tttgcatctt gaggttaagg atggtatttt catctagatc attagaagca taaatcttag
     2701 cctctgtaaa tccagcctca tccagttgct gacggacttt cttagaaatg taggcaatat
     2761 ccccagagtc aatccgcaca cccataaagt taatctgatc acccagctca cgcgccacct
     2821 gaatggcagc tggtacaccg atgcgaaggg tgtcataggt atccacaaga aagacacaat
     2881 ttttgtgggt cgcagcgtaa gccttgaaag cttcatagtc attgccataa acctgtacca
     2941 aggcatgggc atgggttccc aaaacaggaa tgtcaaagag cttacccgca cgcacgttgc
     3001 tggttccatt ggcgccacca atcacagctg cgcgtgttcc ccagatggcc gcatccattt
     3061 cttgagcccg acgtgtccca aactccatca agggttcatc ttcgataacc gaacgaatac
     3121 gagctgcctt cgtcgccacc aaagtctggt agttgacgat gttcaaaaga gccgtttcga
     3181 ccaactgaca ttgggctaga ggtccttcca cctgcacaat cggttcatta gcaaaaacca
     3241 aatccccttc ttgggcagaa cgaacggtca actccaactt gaaattgcga aggtaatcca
     3301 agaacgcccc atgataacca agcgactcca aataggctat atcactatct gaaaaacgca
     3361 agtcttcaag atagttcaca attctttcta aacctgcaaa aaccgcatag ccgttcttaa
     3421 aaggctgttg gcggaaatac acctcaaaga ccgccttctt attgtgaatc ccttggtcaa
     3481 agtaaacctg catcatgttg atctggtaca agtccgtgtg caatgtcaaa ctatcatctg
     3541 gatacatact tttcctactt ccttagctag aaacccatga aaattttcaa gaactttcat
     3601 gtattccaat aaattagtac tattatatca cattttagct ggattgagaa aagagtaaca
     3661 agctattctc cactctccaa ttcatccata tcttgttcaa attttttctg agcccattcg
     3721 ccatagctct taagaccaag attgccaata aagatccacg gaaggtaaat gatataagta
     3781 atgacccaag cagacaggta tttaaaattc aaaggattgt gctgataaat ttctatgttg
     3841 aattgataat tctgcaacat caaaagagcc gtaatagcca aggttaggaa aaaacaaccc
     3901 aaaatcgtaa aatgaaaacg accatagtag gtcactccca gataacgggc acgattgaaa
     3961 aagtaaaatg tccctatgat gataacgatt agcagcatat tagaattaaa aaggcttggt
     4021 gctaatactg aaatgatata agataggagc gacaaagcaa tgcagatata gaaactttca
     4081 gagcccgctt tattgaacag ttgttcttct ctttcgtcta gtaattgaga ataataaaat
     4141 ctatttttca tcttcttcct cccaaaatag ttggtctagg gttttcccta aacatctgca
     4201 aatagactgg cagagcgaga gactgggatt gtattttccc gcctctatta aaccaatagt
     4261 ctggcgtgtc accccgacag cctctgccag ttgaccttgt gttaaatcac gctctacccg
     4321 agctaatttt aattttaaat ttttagccac cttcgtcctc cttatagttt taatactcat
     4381 ctactcttaa aaaatccaaa accaacacaa gctatcagct atttattata accccattaa
     4441 tttgcactcc agtattatct tcagccgaaa atcagtattt taggttgttt gaaataaact
     4501 tcctagttta ctcttttatt tttatttagt attagcttta gtaagatctt ttaaacgaat
     4561 ttttattgct tatatttctt gtttagttta gttggtttca tatcactatt atataatgct
     4621 tttggaaatg agcctagtat aagtcaaaaa cgtgttgaat agtgaaagca ggagcatacc
     4681 aagatgactg attttttact gtattttagg tatgcaaaat aatactaaat gaaactaaaa
     4741 tgaatacttc ttgcttttct tgagagtgaa aaagtacaat aaaatgattg tagtgattag
     4801 cacaagatac tgactaatag acatcgtatt tgaagccaca aatacaggta aagaataaaa
     4861 aaggaagaag ttcaattgct ttgataaaca aatcattact agtattcatc atcatcgtat
     4921 ttaatatatt agagataaag agtagtaaag ctactaaaat accaaatacc aagccacttt
     4981 tcaatttgta taaatagaaa atgtaagata atagaaaata aaacaggact aacaaaattg
     5041 tattgactac aatagctact ataaaattta atcctaaatc acctggaata cccaatttac
     5101 ttactatgac tagtaacaca atgccattca aagcataaat aatggaaact gagatagcat
     5161 atagaaaatt agacaagacg taaatcatcc gattcttctg tttatttaat ttcatgatta
     5221 taaccttcct ttattttgaa cgatttaggt aaatatcttt aagcgtctct tttttagttt
     5281 caaaatcaac tactttaata gagttttcgt taaaaaattt aaaaagctca ctattttgaa
     5341 tattcccaga catagtaatt ctcaatccct cttcctaaac aatatcccaa aattcttgtt
     5401 tggtaatgaa aatgtctcta tctgtagctg atgaaaaagc tatctcaaac aagaaattat
     5461 gactatcttt tcccactttt tgaaatgtca aaagcccgtt ctccaagaaa agaactctct
     5521 cacaaatttc ttcaatgtct tctaatttat gactcgatat taaaattccc acattttcat
     5581 gtaaagctaa tttttttaga accgctaaaa ctatttgtga tgattcaata tctaaaccat
     5641 tagtcggttc atctaaaatc aatatatcag gttccgtaac gagagttaga agcaaagcta
     5701 atttttgttt tgtacccaaa gaataggtct ttactttttt attaatagac tgagtcaaat
     5761 ctaactcttg gatcaaacat ctaaatcttt cttgattgta gtcaacacca tataaatttg
     5821 acaaatattt taaattctct aaacctgttt tagataaaaa taattttggt tcttcgatta
     5881 aatacccaac attatcactg cttataatat tacctgaagt cggttgatta ttctgaacaa
     5941 gaattttcat caacgtactc ttaccaactc catttctccc tgctagacca acaatttcac
     6001 ccttgttaag tgcaaaattt atatctttga gaatgattga atgaccataa tgcttactta
     6061 cattttttat tcctccatta atattattgg ttttctcttt gattctcatt aattttaaaa
     6121 agttgcgcct ctttttaaca atattatatc atatatctaa cttaatgcaa tatataaatg
     6181 tcattttgtg aaattttatt aacaaaaaac tctctacaac aaaataatag agagttttct
     6241 gcttattaat ttttttcaaa atatctaaga catcgcttct ttgtttaatt tgataggctt
     6301 tgatgcctaa actccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc
     6361 atcaagggat ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcatag
     6421 ttttttaaaa ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta
     6481 taaagattga taagcccctc tttaagagct cgaaccatgt ctcctgattg aacaggttgt
     6541 gggactttaa tagtcaatag ttccattgga tttggagcgc ggtcgatttt attgccttta
     6601 gcatcatgca aatcttcaat ataggtttca aaatgacgga aacctggacc ataaaactca
     6661 acttggtccc cttcgttaat gacgtttcgt tgacgaatag ttgctgtttg tgccgcatca
     6721 tcataagaaa ccacttcagc gacaaacttg tactcaggga ttttacgacg agcaccaaac
     6781 aactgctcat tttcagatgg tgtaccatag taaaatcctg tagccagttc acgttgggca
     6841 accttccaca tctcgtccac caagtcttgt ttgatagctt caaacttttc aggactttca
     6901 agataggcat ccacagccgc cttgtagcag ttggttactg ttgatacata gtgaatagac
     6961 tccatacgtc cttcgatttt tagactgtcc acaccatttt caatcatatc tgagatatgg
     7021 tcaatcatag acatatcaac ggctgacatt gaaaattctt ctggaatctc accctgcaaa
     7081 ctcttacgtt ctttcccaaa tggcatatcg taaaggtcgt acttccaacg gcatgactga
     7141 gaacatccac cacggttggc atcacgcata ctctagacgg attgttttga caattctaac
     7201 cttttagact ttgcataata actattaaac agtcgaaaat taacccagtt ttaacaagta
     7261 gtcaaaatac cagataaagg aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt
     7321 attgaacaat cttgatatta tgcgttttat tgtgggaaga tttacttcat tttctcttga
     7381 aattgagttt tctcctactt caaaactaga tgatttgcta tttaaaatag cgttccttat
     7441 gattttggca acctggatta aagcgagaaa aacaaaaggg gcaacttaag ctttctttat
     7501 attgcttata tgttagtagt tttagacaaa caccacataa aataggctta tcctgtatca
     7561 aagataaggg atacggctca aaagggtgat gttctaatga atcatgacac cgataacaag
     7621 cataatactt tttacaatca tagcattgta aagcaataat atccagcttg ctatggtaat
     7681 gaatgcatct tgtttgctta tccactaaga aaccctgtgc ctctatcatt tccttttcct
     7741 ttcttaagct cgaactgtga cgctggttcc atcttcctta taaagattga tgagtccttc
     7801 ttttaatgca cgaaccatat ctcctgattg aacgggttga ggcaccttaa tagtcaatag
     7861 ttccattgga tttggagcgc ggtcgatttt attgcctttg gcatcatgca aatcttcaat
     7921 ataggtttca aaatgacgga aacctggacc ataaaactca acttggtccc cttcgttaat
     7981 gacatttcgt tgacgaattg ttgctgtttg tgccgcatca tcataagaaa ccacttcagc
     8041 gacaaacttg tactcaggaa ttttacggcg agcaccaaac aactgctcat tttcagatgg
     8101 tgtaccatag taaaatcctg tagccagttc acgttgggca accttccaca tctcgtccac
     8161 caagtcttgt ttgatagctt caaacttttc aggactttca agataggcat ccacagccgc
     8221 cttgtagcag ttggttactg ttgatacgta gtgaatagac ttcatacgtc cttcgatttt
     8281 tagactgtcc acaccatttt caatcatatc tggaatgtgg tcaatcatag acatgtcaac
     8341 ggctgacatt gaaaattctt ctggaatctc accctgcaaa ctcttacgtt ctttcccaaa
     8401 tggcatatcg taaaggtcgt atttccaacg gcatgactga gaacatccac cacggttggc
     8461 atcacgcata ctcatgtggt ttgaaagagt acaacgtcca gagtatgaaa tacacatagc
     8521 tccatggaca aaggcttcaa tttcaacatc tgtacgtttg cggatctcag ctaattcttc
     8581 cattgaaacc tcacgcgcta aaacgacacg agtcaagcct agctctttcc agaactcaag
     8641 ggtttcatag ttagtggcac tggcttgggt agaaaggtgg atttcaaggc ctggtgcttc
     8701 agtcactgca atcataatca aggctgggtc agatacgata actgctgcaa tcccgatatc
     8761 acgcagttta cggaaccact caccagcacc agcttcattt ccttcgtgca taaccatatt
     8821 agccgctaca tagaccttgg caccatactt ggccgcaaac tgcacgcctt cttccatctg
     8881 ttcgaaagta aagtttcccg cacggctacg aagaccatag gcctgaccac cgataaagac
     8941 agcatctgct ccatactgaa cagctacctt tagcttctct aaagtccctg caggtgataa
     9001 aacctcagga cgttttaatg tttttgtcat gatttctcct atttgcaata taaaagtttg
     9061 acatttttcc ttctcatttt atagtaaata aaggataaaa tcaagcctag acggattgtt
     9121 ttgacaattc taatctttta gactttgcat aataactgtt aaacagtcga aaattatccc
     9181 agttttaaca agtagtcaaa aagtctagaa aagtaaaaac cagtatccca agatactgac
     9241 tcgtaaaaat acataaatta gtccttagat gactactccc actctgttgc tcctataatg
     9301 gaaaaggatt gatacagtct agaatatcgc ttttttattt attattctga tataaaactg
     9361 ttagaattga ttttttgttt tatacccaaa tatgaataga gacgtagcta ataagttgaa
     9421 taacatattt ggaaaaatga gaaatagcaa tattactaga acttttagaa acaatcaata
     9481 tttaaaaaga aaaaagctca aagaatttat taaaccatgt tataatagat atagctataa
     9541 ttttatttat aacaagagga tagaaatgac cgaattagaa agaaaaaatc gaaaaattag
     9601 ctaagaaata ttctgataac ttaaacatca aagttcaaga gagagttcgt gaaatggcaa
     9661 atgataataa gagccattat ttgatataca gagttttagg tatttcattt gaagaaggag
     9721 aaaatatcga tttgtatcaa aataaaggtc gttttttata caaatatgct ggttcatttt
     9781 tagaagaagc tgcagtacta tgctttaacg aaaaatttgg tacagaaaat acttaaaaag
     9841 ttaacattcc taattctgaa agtacaaaac ctaagacttt tgaaattgat tgtttagtcg
     9901 gagaaaaaca cgcatacgaa ataaaatggt gggatgcaac tacagatgga gaccatataa
     9961 ctaaagaaca cactagaata aaagttattc ataacaaagg atatatacca attcggttaa
    10021 tgttctacta tccaaataga actcaagcta taaaaattca gcaaacttta gaaacattgt
    10081 ataacggtat tggagggaaa tattattatg gagattctgc ctgggaacat ttaagagcag
    10141 tgaccggtat tgatttactt agtattctaa cagatattgc aaataaaaaa acaggggtaa
    10201 aatcaaaatg acagtattaa aaggagataa cttagaaata ttaaaaacta ttgaatcctc
    10261 aagtattgat ttaatctata tggaccctcc tttctttaca cagaaaaccc aaaaattatc
    10321 taataacaaa aatattatgt attcattcga agatacgtgg acttcgattg aggattacaa
    10381 agaatttttg tctgtaagat tagaagaatg caaaagagtg ctaaaaaata gtggcagtat
    10441 tttcgttcat tgtgataaaa ttgcaaatca tcatattaga ttaattttag ataatatctt
    10501 tggagtagat atgtttcaaa gcgaaattat atggaactat aaacggtggt ctaattcaaa
    10561 aaagggatta ttgaacaatc atcaaaacat ttacttttat tcaaagtcaa aagattttaa
    10621 atttaataca atttttacag agtattcttc tactacaaat atcgaccaaa tactagtgga
    10681 acgaaaacga gatggaaact ctaaaactat atataaggtt gataataatg gtaactatat
    10741 tctagcaaaa gagaaaaatg gagttcccct ttcagatgtt tggaatatac catttcttaa
    10801 tccaaaagct aaagaaagag taggttatcc tacacaaaaa cctattctgt tattagaaca
    10861 aattataaag attgctactg ataaaaatga tatagtttta gacccgttct gtggaagtgg
    10921 aactacttta gtagcctcca agattttgaa tagaaattat atggggattg atttatctga
    10981 ggaagctatc aatataactc agcaacgtct ggaaaatgtt ataaaaacaa gttcaaattt
    11041 attgaataaa ggaatcgaag catatagaac caaaactgag gaagaggaaa acattcttaa
    11101 attattacag gcaaaaattg ttcaaagaaa taaaggaatt gatggttttt tacctaaaca
    11161 ttttcaaaaa aaaccgatac ctataaaaat tcaaaaaaat aatgaatgtc tgaatgagag
    11221 tatctcttta ttacagaatg ctataaactc caaaaaactt gattttggag tagttataaa
    11281 aactcattca gataatttat tatttgattt tgatacgatt cctgaaaata taatagtagt
    11341 tgaccatttt gaacttacaa tcgaaaaatg gttaagtaaa agtcaacagt tactatagtt
    11401 tgcaaattat ttacagaaag aacaaaaaat gctctgaaac actgatttca aagcattttt
    11461 tgttagttta aaattactcc cattcttctt ttccaaacgt acaatatatc caaaaccatt
    11521 caaaatacta gattctattt tttataatat cactaaatcc acctaattat aggacgtttt
    11581 cagattttta gtcccagtcc cagtaccgga gaaatattgt tttaatataa tatctctttt
    11641 tgtcttctaa gctcttaaaa gcaaaagaac aagtaaagag tcaagacaag gataaaaagt
    11701 ccatattagg gcaaataaaa agctttaaga cagatgacaa atctaagtca aataagaaag
    11761 accatagcaa aggtgcagag agataaatat tggcggtctt cggactgcct ttattttttt
    11821 atccattttt caaatcaaat ttattcagac tatatatgca catatacact taaattcata
    11881 taaaaacatg gcttgtaaaa aattacttta atcacaataa tcgcatttaa aattgtgatg
    11941 tttgcaagct aaattacgga cttcacttgg aagttttccc ttgtatcttt tataatagat
    12001 agaaaatttg ctggcagatg aatatccaac agattctgct atctctttta taggtagttc
    12061 agtgtttaaa agaagagttt cagctacatt cattcttttt ctttgagtgt actctgtaat
    12121 gctttgacaa tatttttcct taaataaatt ttttaattta gtaccactca ttttagatat
    12181 tttttcaagc gtgccttgat ttacattcgt tgcaaaatga tcatctaaga atcttgctac
    12241 atcttcaagt gctttatcat catcaatttc aattttatat ttttttctat ttaagtatgt
    12301 gtcaattact atacttatcc attcatttgc ctttgcttta aagaaaaaat cagcggcagg
    12361 agcgtccatc ttacaattta atatttccat tgccactctt tctaaggcct ttgtaagtat
    12421 tatttgattc ggttgaagca aggttgaata aaaagattct ggattaatgt taatagatgc
    12481 taaatgtttt tctattagct cttttttaaa accaatggaa acagcaagat aacaacaatt
    12541 ctcgtgtaat aaaaaaacaa aattatcttt tatattatca aaatcaaaag tacatagaga
    12601 gtttgcggta atagtttgat acggattaaa cttttctccg tttgcactga caatgtaact
    12661 tgaataaatt gaaacatagt ctgacatact ataagtgcta ttttgaacta cttcctcttt
    12721 gatataaaaa tcatgtatat cgataatgaa gctgcctcct tcataaaacc aatatagtcc
    12781 ttctgcataa gttgaattct cttttttcca acaaaatgta tgacctgcac ttgaatactt
    12841 ctcattgttt ttacattcat ctactttcat ataatctttt acaaaatcat aatatgacat
    12901 aacacactat cccttttaga caatattcca attagcctta ttaattcaaa actattgtat
    12961 tagtaattat aacagatgta taatagaaaa gcaatgatag atattatcaa ttaagcgaat
    13021 ttatatctaa aagggatatt aaagaaagga gatatgctta tgaagattta caaaaaacta
    13081 tttgcttatg tccaagataa gaaatatctt ggggttttgg ccataatttt ttctgctata
    13141 tctgctgcac ttacagtata tggatattat ttaatctaca aatttctaga taagttaata
    13201 attaattcaa acttatccgg tgcagagagt atagcattaa aatctgttat tacactaaca
    13261 agtggagcga tattttattt tgtctcagga atgttttcac atatcttggg attcaggctt
    13321 gaaacaaatt taagaaaaag gggaatcgat ggtctggaaa aagcaagttt taggttcttt
    13381 gacttaaatc catctggtca aataagaaag attatagatg acaatgctgc acaaactcat
    13441 caggtggtag cacacatgat tcccgatagt tctcaggcaa taatcacacc cgtacttgta
    13501 cttgcacttg gctttatagt aagtataaga gttggcataa ttttgcttgc tcttactata
    13561 attggtggct taattttagg ggcaatgatg ggcgagcaag aatttatgaa gatataccaa
    13621 gaatccctat ctaaactaag tgctgaaact gttgagtacg tgagaggaat gcaagttgta
    13681 aaaatattta aagcaaatgt agagtctttt aaaagctttt ataaggcgat aaaagattac
    13741 tcaaagtatg cttatgatta ttccctatct tgtaaaaggc cttatgtttt gtatcaatgg
    13801 ttattttttg gactgattgc aattttaatt attcctatag tttattttat gactagctta
    13861 gctagcgcaa aggtgatttt acttgagctt atcatgattt tatttttatc aggagttctc
    13921 tttgtttcat tcatgagaat gatgtggtac tccatgtata tttctcaagg aaattatgca
    13981 gtagatactt tagaggcgct ttacgaagat atgcaaaaag acaaattagt gcatggtaat
    14041 gtcaataatt ttaaaaacta taatatagaa tttgagaatg ttagctttgc ttataatgat
    14101 aaagctgtca ttgaaaattt atcctttaat ttagaagaag gaaagtccta cgcacttgtc
    14161 ggttcatctg gatcaggcaa atcaacagta gcaaaactta tatcaggttt ttacaatgtt
    14221 aataaaggaa gcataaagat aggcgggata gcaataagtg aatattctga cgaagcctta
    14281 attaaagcca tttcctttgt ttttcaagat tcaaaattat tcaagaagag catttatgat
    14341 aatgtagcgt tagctaataa agatgcgacg aaagatgacg ttatgagagc cttaaaatta
    14401 gcaggatgcg atttaatatt agacaaattc ccagaaagag aaaatacaat cataggctca
    14461 aaaggtgttt atttatccgg tggagaaaaa caaagaattg caattgctag agcaatttta
    14521 aaggattcca aaattattat tatggatgaa gcatcagcat ctattgaccc agataacgag
    14581 tttgaattgc aaaaagcttt taaaaatctt atgaaggata aaacagttat catgattgca
    14641 cacaggctat ctacaattaa agaccttgat gaaattattg tcatggatag tggaaaaatt
    14701 atagaaagag ggtctgacaa agaattaatg tcaaaagata caaggtataa gagcctgcaa
    14761 gagatgttta acagtgcgaa tgaatggagg gtttcaaatg aaagagtttt ataaaaaaag
    14821 atttgctctt acagatggag gagcaagaaa tttaagtaaa gcaacactgg cttcattttt
    14881 cgtttattgt ataaacatgc ttcctgccat attacttatg atttttgctc aggaagtttt
    14941 ggaaaatatg ggcaaaagca atggctttta tatagtattc tcagttttga ttttgatagc
    15001 aatgtatatt ttgctttcta tcgaatacga taaattatat aacacaacct atcaagaaag
    15061 tgcagattta agaataagga cagcggagaa tttatcaaaa ttacctctat cttacttttc
    15121 taaacatgac atttccgaca tttcacaaac aatcatggct gatattgaag gcatagagca
    15181 tgcaatgagc cactcaatac caaaggtggg cggcatggta ctgtttttcc cattaatatc
    15241 tgtaatgatg ctagcgggca atgtcaagat gggtttagct gtaattattc catctatttt
    15301 aagctttata tttatacctt tatctaaaaa atatcaggtt aatggacaga atagatatta
    15361 tgatgtctta agaaaaaact cagaaagctt tcaagaaaat atcgaaatgc aaatggagat
    15421 taaagcatat aatttatcga aggatattaa agatgactta tataaaaaaa tggaagatag
    15481 tgagaaagta cacttaaagg cggaagtaac tacaatttta actttgtcta tatcttcaat
    15541 atttagcttt atatctcttg ctgttgtgat atttgtcggc gtaaatctaa ttattaataa
    15601 agagataaat tctctctacc ttataggata tttactagct gctatgaaga taaaagactc
    15661 tttagatgca tctaaagagg gcttgatgga aatattttat ttatcgccca aaatagaaag
    15721 attaaaagaa attcaaaatc aagatttaca agaaggcgat gactatagct taaaaaaatt
    15781 tgatattgat ctaaaagatg ttgagtttgc ctacaataaa gacgcaaaag ttttaaatgg
    15841 tgtaagtttt aaagctaagc agggagaggt cactgctttg gtaggtgcaa gtggctgcgg
    15901 taaaacaact atcttgaaac ttatatcaag actttatgat tatgacaagg gacaaatctt
    15961 aatcgatggc aaagatataa aggaaatatc aacagaatcc ctttttgata aggtgtctat
    16021 tgttttccaa gatgtggttc tctttaatca aagcgttatg gaaaatatta gaatcggtaa
    16081 gcaagatgca agtgacgaag aggttaaaag agcagcaaaa cttgcaaatt gcacagattt
    16141 tatagaaaaa atggataaag gtttcgatac agttattggt gaaaacggag ctgagctatc
    16201 aggaggagaa agacaaagat tatcaatagc cagagccttc ttaaaagatg cgccgatatt
    16261 gatcttagat gagataacag caagccttga tgttaacaac gagaaaaaga ttcaagagtc
    16321 tttaaataat ttagttaaag ataaaactgt tgtaatcatt tcacatagaa tgaaatccat
    16381 agaaaatgca gacaagatag tagttcttca aaacggaaga gtagaaagcg aaggtaagca
    16441 tgaagagctt ttacaaaaat caaaaattta caaaaattta atagaaaaga caaaaatggc
    16501 agaagaattt atttattagg aggactacaa tggataataa aaaattaaaa gtaaaagatt
    16561 tagtaagcat cggtgttttt ggcgtaattt attttgcctt catgtttgga gttggtatga
    16621 tgggcttgat tccaatattg ttcttaatat acccgacagt attagccata gttgcaggaa
    16681 ctgttgttat gttatttatg gctaaggttc aaaagccatg ggcactattt atatttggta
    16741 tgatatcacc acttgtgatg tttgcagctg gtcataccta cgtagttgtg gttttatcac
    16801 ttatagtaat gataatagca gaattaatta gaaagattgg taattataat tcatttaaat
    16861 acaatatgct ttcttatgca atcttcagca catggatatg tagctcttta atgcaaatgc
    16921 ttttagcaaa agaaaaatat atggagtggt ctttgatgac tatgggaaaa gattatgttg
    16981 atgtattaga aaagttaata acttatcctc acatggcttt agtagcctta ggtgctttct
    17041 taggaggaat tcttggagca tatataggca aggctctatt gaaaaaacac ttttcaaatg
    17101 gattatattg tgtgggatac tttactcctt gcctaatttt atggtgctat ctgaattaaa
    17161 ccctatagtt aagatgtttt tgagtatacc tattgttatt agaatgttta ttttaccatt
    17221 tatggcagca agctttatga taaagacctc ggatgtaggc gcaataattt catcgatgga
    17281 taagcttaag atttcaaaga atgtatccat acctattgcg gttatgttta gattcttccc
    17341 atcttttaag gaggagaaga aaaacatcaa aatggctatg agagtaagag ggataaattt
    17401 taaaaaccca gtcaaatatc ttgaatatgt ttctgtgcca ctactcatta tatcatctaa
    17461 tatatcagat gacattgcaa aagcggcaga aacaaaggca atagaaaatc caattgccaa
    17521 gaccagatac attcgcgtaa agatacagct aattgatttt gtttatgttt tagcggttgc
    17581 tggacttatt gtgggaggct taatatggtt gaaataaaaa atttaagtct tgattatggt
    17641 gaagagcata tattagatga tatatcacta tccatagccg agggagagtg cgtgctattt
    17701 acaggaaaaa gtggaaatgg taagtcatct ttaataaatt caatcaatgg actagctgta
    17761 aggtatgata acgcaaagac aaagggcgaa ataattattg atggtaagaa tataaaaaat
    17821 ttggaacttt atcaaatctc aatgcttgtt tcaactgttt ttcaaaatcc taagacatat
    17881 ttttttaatg tcaatacgac attagaatta ttattttatt tggaaaatat cggtcttgca
    17941 agagaagaga tggacaggcg tttgaaggat atacttgaga tattcccgat aaaaaatctt
    18001 ttgaacagaa atatatttaa tctatccggc ggtgaaaaac aaattctttg cattgcagct
    18061 tcttatatag caggtacaaa gattatagtt atggatgagc cttcatcgaa tttagatatt
    18121 aaaagcataa gtgttttggc aaagatgcta aagatattaa aagagaaagg cataagcata
    18181 attgttgcag agcatagaat ttattatttg atggacatag ttgaccgtgt atttttaata
    18241 gataaaggaa agcttaaaaa aacttatact agaagtgaat ttttaaagct agataaaaat
    18301 gaattaaatg ctttaagttt aagagataaa gaattaagta aattaaaagt tccttattta
    18361 aaagaaggtg gagagtatca gataaaaaat cttagttaca aatttactga tgatgagtgt
    18421 ttaagcttaa aagatatttc gttcaagctt gggaaaattt atggcataat aggatccaac
    18481 ggacgaggaa aatcaacgct tttaagatgt ttaataggtc ttgagaaaaa atcaaaagaa
    18541 gaaatttatt ttaagggaga gaagctatct aaaaaagaaa gactcaaaaa ctcttcactt
    18601 gttatgcaag atgtaaatca tcaattattc acagatgaag tattcaacga gcttagatta
    18661 ggagtaaaga attttgatga agaaaaggcg aaaatca
//
LOCUS       DBDESG010000049        18490 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.049, whole genome shotgun sequence.
ACCESSION   DBDESG010000049 DBDESG010000000
VERSION     DBDESG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.049"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(122..1972)
                     /locus_tag="ACKB38_001617"
     CDS             complement(122..1972)
                     /locus_tag="ACKB38_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGL7980360.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2014..3171)
                     /locus_tag="ACKB38_001618"
     CDS             complement(2014..3171)
                     /locus_tag="ACKB38_001618"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7980361.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKEDIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFILFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNML"
     gene            3229..4395
                     /locus_tag="ACKB38_001619"
     CDS             3229..4395
                     /locus_tag="ACKB38_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGL7980362.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="ACKB38_001620"
     CDS             4534..4860
                     /locus_tag="ACKB38_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGL7980363.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="ACKB38_001621"
     CDS             4847..5440
                     /locus_tag="ACKB38_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGL7980364.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYATRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="ACKB38_001622"
     CDS             5433..6344
                     /locus_tag="ACKB38_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGL7980365.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="ACKB38_001623"
     CDS             6407..7471
                     /locus_tag="ACKB38_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGL7980366.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7649..8509)
                     /locus_tag="ACKB38_001624"
     CDS             complement(7649..8509)
                     /locus_tag="ACKB38_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGL7980367.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8599..8724)
                     /locus_tag="ACKB38_001625"
     CDS             complement(8599..8724)
                     /locus_tag="ACKB38_001625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980368.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSYIYLRQGEADAV"
     gene            complement(8784..9770)
                     /locus_tag="ACKB38_001626"
     CDS             complement(8784..9770)
                     /locus_tag="ACKB38_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGL7980369.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(10042..11517)
                     /locus_tag="ACKB38_001627"
     CDS             complement(10042..11517)
                     /locus_tag="ACKB38_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7980370.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11775..12698)
                     /locus_tag="ACKB38_001628"
     CDS             complement(11775..12698)
                     /locus_tag="ACKB38_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7980371.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLIGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12712..13641)
                     /locus_tag="ACKB38_001629"
     CDS             complement(12712..13641)
                     /locus_tag="ACKB38_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980372.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13960..14181)
                     /locus_tag="ACKB38_001630"
     CDS             complement(13960..14181)
                     /locus_tag="ACKB38_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980373.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(14135..14395)
                     /locus_tag="ACKB38_001631"
     CDS             complement(14135..14395)
                     /locus_tag="ACKB38_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980374.1"
                     /translation="MTGVSNPTTDSARLVLAEANKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            14475..14612
                     /locus_tag="ACKB38_001632"
                     /pseudo
     CDS             14475..14612
                     /locus_tag="ACKB38_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(14716..14871)
                     /locus_tag="ACKB38_001633"
                     /pseudo
     CDS             complement(14716..14871)
                     /locus_tag="ACKB38_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(15049..15660)
                     /gene="rpsD"
                     /locus_tag="ACKB38_001634"
     CDS             complement(15049..15660)
                     /gene="rpsD"
                     /locus_tag="ACKB38_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGL7980375.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(15855..16907)
                     /locus_tag="ACKB38_001635"
     CDS             complement(15855..16907)
                     /locus_tag="ACKB38_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGL7980376.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(16904..17602)
                     /locus_tag="ACKB38_001636"
     CDS             complement(16904..17602)
                     /locus_tag="ACKB38_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7980377.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(17769..>18490)
                     /locus_tag="ACKB38_001637"
     CDS             complement(17769..>18490)
                     /locus_tag="ACKB38_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HGL7980378.1"
                     /translation="VPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDK
                     APWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYS
                     ATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT         5424 a         3992 c         3450 g         5624 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tgtgtaactt gatactatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaagtaac gggtcatacg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     1741 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     1981 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     2041 tattttatcc aaataaatta ttgttctctt gtactccttc ttccatttaa atcctctaat
     2101 taaacctaca atcgtcccaa ggccataagc aaagtgaatg gaaaataaaa taaagggcat
     2161 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta atgacaaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatat tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac attccatgaa aaatagaaga
     2581 aacatatcta tcctcagaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     2701 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaagtctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tatcctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     3121 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gtgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctgttcc actcttgggt accatctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatgggca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaattctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgcaa ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg caaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatt
     6361 tgctactatg gaaacatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttgaaaaaaa
     6541 tactcagtta gaggaggaag tccctcaagc tgaagtcgaa ttggaaagcc agcaagaaga
     6601 gaaaattgaa gctcctgaag acagtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttaca gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aacttcatac tcttcaaaaa tcaaattcaa
     7501 accacgtcag cttcgcctta ccgtactcaa gtacagcctg cggctagctt cctagtttgc
     7561 tctttgattt tcattgagta gcaatcctgt catccttggc aggatttttt ctatgtcaaa
     7621 aaagcaagtc gattgacctg cttctgcttt acttttcttg tgctaatgtt ttaaaatctt
     7681 tgtctaatat gggttgcact tctatttgat tggcgtctag ttggtggtaa aaagatgggg
     7741 gtaatacctc taagaatttt gcatagtcca agcgagcaat ggcttcatag tcttctggtt
     7801 tagagccatc tcctgtgatt tgaaccaagt caatctccca ctggacttcc ttatccacca
     7861 attgctcaat ataggctgca ggaacaaacg gttctctcgg taaaactgtc ttgactcctt
     7921 gagctagatg gtaaacgcca tgcacttttc cttctccatc ctgatagaag gaaactctcg
     7981 caaagtaggc atctgtctct tgaaccgcac gcacacgcgc ttcaaactga gccaagccat
     8041 ctgcctctaa aaagcttttc aagttctcac gactaaagaa aataggatta aaaattcctt
     8101 ggcaaatcgt aaaacgagct acagggtctg ctagataggc ttgaatactt gcttggaaat
     8161 aagcttcaaa atcaaaacca tctagccctt caatatcagt ccctgtataa gcaagcaaag
     8221 catctaaaaa tgacttgata atctgccagt ctgtctttgt tagtaggtca ggaagatcga
     8281 cacggtaagc cttttgacca tcagcataac tgaccttaaa gagaaattgt gattgcccca
     8341 ctatcgcaca ctcgatatat tcgagacgat taagaggctg acggagatag actacatcat
     8401 agctatgtga ctccaaaccg tctagcaagg ccaagatcga cttggcattc aaaatctctt
     8461 gttctcctaa aatgctctgt ttatttggaa taaaaaatgt tttcgccata actggactcc
     8521 tttgaaatcg tttacataca gtatacctta tttttctgaa agatacaaaa acaaacttta
     8581 tactcttcga aaatctcttc aaaccgcgtc agcttcgcct tgccgtagat atatgtaact
     8641 gacttcgtca gtcttatcta caacctcaaa gcagtgcttt gagcagcctg cagctagctt
     8701 cctagtttgc tctttgattt tcattgagta ttagattttt gagtacaaag catagaaaaa
     8761 cagcccctga ggactgtttg atcttataca gtagctgctt gatccaagct ttcaccgata
     8821 gcggctaggc gctcgataac ttcagcttgt gtcaattcat tttttgaaac atagcggtta
     8881 cgtgggtgaa cacggcactc gtgtgagcat ccacgaaggt acttgtcttc attttcttct
     8941 gatgtcaaga tacgacggtt acagaatgga tttccacagt tgacataacg ttcacatggt
     9001 gttccatcaa accagtcttt ccctacgata gttgggttga catggttgac atcaacggca
     9061 atacgctcgt caaagacgta cattttccca tcccaaagct caccttgaac ttctgggtct
     9121 ttaccgtaag ttgcgattcc tccgtgcaat tggccgacat ctttgtagcc ttcacggacc
     9181 atccagcctg agaatttctc acagcgaacg ccacctgtac agtaaaccac gacacgcttg
     9241 tccatgaatt tttccttgtt atcacggacc cattgtggta actcacggaa gttgcgaata
     9301 tctgggcgaa tagctccacg gaaatgtcct aggtcgtact cataatcgtt acgtgtgtca
     9361 aggacaacgg tatctttatc aagaagcgct tctttgaact cttttggaga caagtaagca
     9421 cctgttgttt caagtgggtt gatgtcattg tcaaagtcgt tgtcttccaa accaaggtgg
     9481 acaatttctt tcttgtagcg aacaaacatc ttcttgaagg cttgttcatt ttcttcgtca
     9541 atcttgaacc agagttcttc cattcctgga aggctgtgaa cgtagtccat gtatttttga
     9601 gttgtttcat agtcacctga aactgttccg ttaattccct cgtcagcgac taggatacgg
     9661 cctttaaggc cgattgattt acagaaagcc aagtggtctg cagcaaattg ctctgcattt
     9721 tcaattggag tataaaggta gtaaagtaag acacgaatat cttttgccat aagatttgtt
     9781 ctcttttcta ttcttaaatt ttctaaattt tttatttatc tacactagta tacctgcttg
     9841 agaaaacgaa tgcaatttat ttgactggaa attgtagagg gttctcgaaa tttcttgaat
     9901 ggttaaaata aggacaagag aaaacatgga tatctatatc cttgtgccaa aaaaaccact
     9961 gccctcccca gaccaacctg aggaaagcag tgattcttat tttaggagtt aggaatgaat
    10021 acacgaaatc aatttagctg attatttttt gtttttcaag aattcatcgt attgtttttg
    10081 catttcgttc aatacttttt cgtaggcacc ttcagatttc aatttttcca tcaattctgg
    10141 aatcgcttta tctgggtcta cagtaccagt gttgatagct gtatcaaatt gttgcattgt
    10201 gttagcaata gctgagattt cagatttcac attgtcagta ttgaagataa atccaagcgc
    10261 tggagattct ttagcttctg ccaattcttt cttagaattt tcgatttgtt ggtctgtaac
    10321 gttttcgttg atgtaaagga tccagttgtt accagtgttc catccaccca tgtgagtgtt
    10381 tcctttgtag ccatcaagaa cgcgaacacg gttttcttta ccttcaattt tttcccagtt
    10441 cttgccttct ggaccgtaaa caagaccgtt caagagttct gggttcgtat tcaagaggtt
    10501 caagatttcc attgattttt ctttgttctt agagttgttt gagatgacaa agttagcaac
    10561 ttgtgttgtt tggtttttct tgatgaagtt agtaattggt ttgatttgga tatctttgtt
    10621 ggcaacacgt gaaagcaagc tgttaccgta gtcagctggt cctactgttt cttcacgaac
    10681 gaaccaagta tcttgttgaa ggtcaaagga agtatcgctt gttgcgacgt cttttggaat
    10741 gtagccagct tcatagaatt tgtgaagagt cttcaagtgt tctttgaaac gaggcacttc
    10801 gtaacggttt acaactttag tagtatcgcc ttcaaggtcg ataacgaatg gaagaccgtt
    10861 tgctactggg tagtcaaaat tatcagatgg gatgaaaact ttaccaatag caaatggtac
    10921 tacgtctgga gctttttctt tgatttgttt caagactggc tcaagagttt cgtaagaagt
    10981 aacacctgaa atatcgatac catatttagc aaggagagtt ccgttgaagg caaagttttg
    11041 agatgatgca acgttggctg caactggaac agcgtaaatc ttaccattta cagtattacc
    11101 cttgatgtaa gctgggtcaa gtgctttgta aaggtcttta ccttcttttt tgtacaattc
    11161 tgtcaagtca gcgtaagcac ctttttgagc atttacaata tagttatctg caaaggcaat
    11221 atcatagttt tcaccagatg atgtgataac tgacattttc ttaccatagt caccccagcc
    11281 aaggtattgg atatccaatt tggcaccaac tttttcttca atgattttgt tggcatttgc
    11341 taacaattca tccaagttgt ctggtttgtc accgatttgg tacattttga taacaggttt
    11401 gtcacctgaa tcagcagctt ttttgctgtt acctgtcaaa tttccacaag cagcaagacc
    11461 tgcagccaga gcgactacac tagcagatgc aaaagcatat tttttccagt ttttcatgat
    11521 aaaaactcct ttttttattt ttaaacttat aaacaatgta atgatcttat actcaataaa
    11581 aatcaaagag caaactagaa aactagccgc aggctgctca aagcactgct ttgaggttgt
    11641 agataagact gacgaagtca gttacatata tctacggcaa ggcgacgttg acgcggtttg
    11701 aatttgattt tcgaagagta ttaacttcac acaagggaag ttgggaactg agaaatgtta
    11761 tttctcaata agcactattc tttcacacca ccgatagtca aaccttttac aaagtagcgt
    11821 tggaaaaatg gatacaaaat cgcgattgga agggttgcaa ccacaaccat ggccatacga
    11881 cctgtttctt tcggtagagc aactcccagt tgaccaatca agccgaccgc tttggcaatg
    11941 tagtccatat tttgttggat ttgcatgagc aaatattgca atggatacaa gttgtcactc
    12001 ttgatgtaaa gaagggcgtt gaaccagtca ttccagaaac caagagctgt taagagcgtg
    12061 atggttgcga tacctggtag tgacaatggc aaacagattt ggaagaaaat ccgggcctca
    12121 ctggcaccat cgatacgagc cgattctaga atggcttctg gaatggtctt cttgaagaag
    12181 gaacgcatca agatgatgtt aaatggtgag agaagcattg gaacaatcaa ggcccaaaca
    12241 gtgtcaccaa gctgaagtac acgggtcacc atgatataac ctggtaccaa accagcgttg
    12301 aacaacatac tgagaaggac gaagatggta aagaatctgc gatacttaaa ggttgtccgt
    12361 gaaatagcgt aggcataggt tgttgtgata aagacatttg tcaatgtccc aactacggtt
    12421 acaaagacag agatgaagag ggcttgtagg attttatcct taaactgtgc caaaaactca
    12481 aaaccgtcta agccaaattg ggatgggaag aagctatagc cgtattggag gaggcttttc
    12541 tcgtctgtca ctgaaataat gataacgaat acaaaaggta ggatacaaga gagggcaatc
    12601 aaacccgaaa tgatactgaa gaagatatct gctttcttac tgaaggagtg aatgccgaca
    12661 ttatcaattt tttctttttt aattttcttt tttgccatat tctcctcctt tctagaacaa
    12721 agctgagttt ggatcgactc gtcttgcaag caagtttgat aggataacca gaatcaaacc
    12781 aacaacggat tggtaaagac cggctgctgc agccataccg atatctgctg tctgagtcaa
    12841 accattaaag acatatacgt ccaaaacgtt ggttacattg taaagctgac cagcattgtg
    12901 tgggatttga tagaagagac cgaagtctgc gcggaagata tttccgactg caaggatggt
    12961 caatacagtt acaagcggag tcaactgagg aatggttacg ttgcgaatac gttgccactt
    13021 gctagctccg tccactgtcg ctgcttcgta gtaggttgga tcaattccca tgatcgtcgc
    13081 atagtacatg acactgctat atccaaagcc tttccaaata cctaggaaaa gtaggagata
    13141 gggccagatg cccaggtcag cgtagaaatt gacttctttg agaccaagac tttccaatag
    13201 atgattgaac acccctttat caatatttag gaaggcatct gtaaagaaac tgatgataac
    13261 ccaagacaag aagtaaggga acaacataga agtttgaaaa atcttcacca ttctcttaga
    13321 acggagctcg ctgaggataa tggcaatccc tacagataca actaaaccta gaaagataaa
    13381 gccaagattg tagaggacag tatttcgtgt gataataaag gcgtctcttg aactaaataa
    13441 gaatctaaaa ttatcgagtc cgacccattt actatttatg atactatcta tgaaaccatt
    13501 actggtcatg tggtagtctt tgaaggcaac cacgttccca aatactggaa tgtaaaagaa
    13561 tagaatcaac cagagtgccc ctggcaaaac catcaagaga aagatccagt tgtctctcaa
    13621 tgtttttgaa aactttttca taatttcctc cctttttatt ttgatatcca tctaaaaatt
    13681 cttttttaga cttttgataa cgattacatt attagtatac tcctatttgc aggttaggtt
    13741 aaactcctaa ttatagaaaa aactccacaa attatgtagc agatttaaaa ctttatcacc
    13801 actatcaaac aaatgtccta aatcaattgt ttattttatc tctattagcc cagtgatggc
    13861 gtcactctgt tataagcatc caacaacggg gtatactgaa aaatctccag actagggaac
    13921 tcagcgatag ttcctaatct ggagattttt aatatgttat taggcgtttg ctttcaactt
    13981 agcaataacc tctttaagat tatcaatcaa ctctgctgca gtatgctcag agcctttttc
    14041 atctgccaag aacaaaactg ctttttgaag ttctttttga gagttttcaa ggacatcctt
    14101 atctactgtt tcaaggtttg agtctttaag aagtttactt aattccttgg ctaatttctt
    14161 gagtttgatt tgcagactca tcttctcctg ctgtttcttt gcccgctgtt tgtcctccat
    14221 ccttagttgc tgactggctt tccttaatgg actctaggga agcaatggca tctttgactg
    14281 tttgcaagat atcacgtaaa ccttgctctg tcaaactatc atctgcaaaa gctttattag
    14341 cctctgccaa aaccagacgt gctgaatctg tggtaggatt cgatacacct gtcaatgatc
    14401 tcaaaagatt ttctaaggtt tgagtctgct tactaatact agactaaaat caaaaagtat
    14461 tatataacag tgatatgaaa tcaactaaag aagaaatcca aaccatcaaa acacttttaa
    14521 aagactctcg tacagctaaa tatcataaac gccttcaaat cgttctattt tgtctgatgg
    14581 gcaaatctta taaagagatt atagaacttt tatagtagtt tgaaataaga tgtgaacatc
    14641 tctatcagga aagtcaaatt aatttataga aatattttag cagccaaggt gtactgttat
    14701 agattcaata cactatactt ggtggtttag ctctcctgtt ttctctttta gctttaacca
    14761 gccataaatg gtattacgtg agatttggaa aacgtgtgat gcttctgtta tactacctgt
    14821 tcgctcacaa taagagagaa cttttttacg aaaatctatt gaatatgcca taaaaggatt
    14881 ataccacatt gtgtactatt tttggttcat tttactatat cactttggac tatatcggtc
    14941 tagttatgtc tcaacgatta tagctggata ctaagagaaa tcaaaaaagc actaagggag
    15001 aacgtcccaa agtgcttaat atcaaggcct atcttattca ataaaatatt acaacatttt
    15061 gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg ttgatttcgt cgcgttctgg
    15121 caagcgagtc aatgaacctt ccaatttttc agcgtcgaat gatacgaatg ctggacgtcc
    15181 aagagtagct tctactgctt caaggattgc tggaactttc aatgattttt cacgaactga
    15241 gatcacttga cctggagtta cgcggtatga tgggatatca acgcgtttcc cgtcaacaag
    15301 gatgtgaccg tggtttacga attgacgagc ttgacgacga gtagtcgcga gaccaagacg
    15361 gtaaacaacg ttatccaaac gacgttccaa aagaagcata aagttgaaac ctaggattcc
    15421 gcctttgatt tttgtagctt gtacgaacaa gttacggaat tgtttttcac ctacaccgta
    15481 agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg tattctgaca atttagaacg
    15541 gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt gccaattctt tacctgtacc
    15601 tgtaagtgaa aggccaaggc gacgagcttg tttccaagat ggtcctgtat aacgtgacat
    15661 atgaatgtcc tcctgatata aataatattt cggtggaaat agtcacttag aaagccctga
    15721 ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt gtcataagac acctgttgac
    15781 gagcttcatg ctttcctgct gatatttcac acaaaatcta ttctaccatg aatagctgga
    15841 tttgtaaagg ggttttaggc tttattttca ctaccagaga ggttgagaac gagggtaaag
    15901 gtacttccta gaccgtactg gctgctgact gtgatttccc cacccaattg atgggccaat
    15961 tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct tcatgttacg cgaagtttcg
    16021 acacgataaa ggcgtttgaa aatattttcc aaatcctctg gggcaatacc ctgcccttca
    16081 tcggtcacac tgattgaaag ctggtccttc tccagcttag ccaccacttc cagcttggtt
    16141 cctggagcag aatatttaaa agcgttatcg accagattca ccaagatacg agaaagctta
    16201 gcataatctc cctcaatccg ggcagactct gggattacct gcaagtggac atctcttctc
    16261 tcctgctcaa tcaaaaactg aaattcactc atgcactcaa ttaagagctt gtccagaaaa
    16321 atactgtctt tactggtagt ttccacctga tttctagctg tgtttagggt caaaaaattc
    16381 aactcctcaa ccagtttatt gagcctctcc gtctggcgtc caatggttgc tagataatga
    16441 gcttgctccg actccttgat aatcccatcc aaaatccctt ctaccgtcgc ttggatcgaa
    16501 gtgataggag tcttaatatc atgcgacaac tgggcaatca tcaagccctt ttctcgttcg
    16561 ctttcttcca aggaatcaaa gcttacctgc aaatcatggg acatctcatt aaaagtttgc
    16621 cctaattgct gaaattctac aggaccttga acctccaaat ttgaaggaaa atccttggcc
    16681 gctacccgct tggcatgctc cttgagtttg cccaacgacg taaagactgg caataggaga
    16741 aagagactaa tcccagcacc gacaaggctg gcaacgatgg tcatcccaag caaaaagtaa
    16801 atctcgcctt tcgcaatcag cattttttga acagcccaaa aaacaaccaa aatggttaag
    16861 agggttgaaa taatatatcc aaccaaaata taacttttta gtttcatgtt tgtcctctcg
    16921 gtttctctat cttatatccc aacccccaaa ctgtcttaat agtgggagtt tggtcactac
    16981 tatattttgc cagctcctgt cgaagagcat ggatatgcac attcaaggta ttggtgtcat
    17041 ccacgtagtc ttctttccag atcttttcat agaggtctgt cttggagaaa actcgctctg
    17101 gattactagc taaaatccac agcaattcaa atgatttaac agttaaatcc agcatttctt
    17161 ctcctatttg aacttcatga ctactatgat tcatttttag attgccaagg gaaatcagct
    17221 ctgtttcgcc cccacgatga aggcggcgca aaatattgtg gacacgcaaa accagctcac
    17281 gtgggctaaa aggcttagca ataaaatcat ctgctcccaa gctcaggccg taaatcttgt
    17341 cctgttcact ggtcttagca gtaataaata ggaaaggctg ctctggtgat aagtattgaa
    17401 cctcactgat taaatcataa ccatccatcc gaggcatcat gacatctgtg ataatcaaat
    17461 caatcggttt tttcttgaac agctctagcg cttccagtcc atcatgggct accaagacct
    17521 gataacctgc ctgaattaag tatctctgat gaatatctgt gatttctacc tcgtcgtcaa
    17581 cgagtaaaat tgtctttccc atctgtctct cctttgataa aaacagtgct atactgcttt
    17641 aagtataaca ctatttttat tgttttacga tgattttaat ctgctattga aagaaagtcc
    17701 tgattgctga ttgagttgtt aaactaatct tagtcagccc actatccatc atttatttcc
    17761 ttagctgatt agttttcttc ttttttgttt ttcaaaccta gaccaccgag taaacctgca
    17821 agcgcaagcc caaggaaacc aatacttgcc attgatgttt gagtctcacc agtatttggt
    17881 agcatagctt tatcctctga catcatcgta tcagacatct tgttagcaga agcagccatg
    17941 ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt cagcaggcat gctatctgta
    18001 gtacctgtag catgattgtg attcatcgga gtcacgccag aaccagagtt agaaggtgat
    18061 aatgaaccat ttgctgtgtc tgatgtttct ttaacattta tcttaatagt gactttttta
    18121 gttgctacga tgttgtccaa gtctggttta ccgtctttgt taccatagac attgactgta
    18181 gcgctgtaag tttgagtacc gtttgctcgg aactggtcaa tgagcgcttg tttttctttg
    18241 ccagctacat ttccgtccaa ggctacttga tagaagtatt gacctttggt cttcacgttt
    18301 tcacctagtg gagatagagc tgggttttta gcgtcgccgt tatctgacca tggtgccttg
    18361 tcagatgcct tgagcaagag acgagtcaac atgccatcac ctgcgaagag ttcgtatgga
    18421 atcacatggt tgacacctgc tgtgaatgga ccttcaccct tggccttttc taggtaggct
    18481 gctggaacat
//
LOCUS       DBDESG010000050        18332 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.050, whole genome shotgun sequence.
ACCESSION   DBDESG010000050 DBDESG010000000
VERSION     DBDESG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.050"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..73
                     /locus_tag="ACKB38_001638"
     tRNA            1..73
                     /locus_tag="ACKB38_001638"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Met,seq:cat)
     gene            87..160
                     /locus_tag="ACKB38_001639"
     tRNA            87..160
                     /locus_tag="ACKB38_001639"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Ile,seq:cat)
     gene            171..260
                     /locus_tag="ACKB38_001640"
     tRNA            171..260
                     /locus_tag="ACKB38_001640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Ser,seq:tga)
     gene            274..347
                     /locus_tag="ACKB38_001641"
     tRNA            274..347
                     /locus_tag="ACKB38_001641"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:Met,seq:cat)
     gene            351..423
                     /locus_tag="ACKB38_001642"
     tRNA            351..423
                     /locus_tag="ACKB38_001642"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Phe,seq:gaa)
     gene            443..513
                     /locus_tag="ACKB38_001643"
     tRNA            443..513
                     /locus_tag="ACKB38_001643"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:475..477,aa:Gly,seq:tcc)
     gene            549..622
                     /locus_tag="ACKB38_001644"
     tRNA            549..622
                     /locus_tag="ACKB38_001644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:583..585,aa:Ile,seq:gat)
     gene            629..716
                     /locus_tag="ACKB38_001645"
     tRNA            629..716
                     /locus_tag="ACKB38_001645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:663..665,aa:Ser,seq:gct)
     gene            complement(842..1777)
                     /gene="birA"
                     /locus_tag="ACKB38_001646"
     CDS             complement(842..1777)
                     /gene="birA"
                     /locus_tag="ACKB38_001646"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGL7980379.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1774..2634)
                     /locus_tag="ACKB38_001647"
     CDS             complement(1774..2634)
                     /locus_tag="ACKB38_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGL7980380.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2742..4904
                     /locus_tag="ACKB38_001648"
     CDS             2742..4904
                     /locus_tag="ACKB38_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGL7980381.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4996..6255
                     /locus_tag="ACKB38_001649"
     CDS             4996..6255
                     /locus_tag="ACKB38_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGL7980382.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            6269..7135
                     /locus_tag="ACKB38_001650"
     CDS             6269..7135
                     /locus_tag="ACKB38_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7980383.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7149..7982
                     /locus_tag="ACKB38_001651"
     CDS             7149..7982
                     /locus_tag="ACKB38_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGL7980384.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7989..8156
                     /locus_tag="ACKB38_001652"
     CDS             7989..8156
                     /locus_tag="ACKB38_001652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980385.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKVKLTWFEEI
                     FEEYKKSSWNL"
     gene            8340..9782
                     /gene="gtfA"
                     /locus_tag="ACKB38_001653"
     CDS             8340..9782
                     /gene="gtfA"
                     /locus_tag="ACKB38_001653"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGL7980386.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQIYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            9920..11113
                     /locus_tag="ACKB38_001654"
     CDS             9920..11113
                     /locus_tag="ACKB38_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGL7980387.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMNSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11798..13777
                     /locus_tag="ACKB38_001655"
     CDS             11798..13777
                     /locus_tag="ACKB38_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7980388.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13844..15340
                     /locus_tag="ACKB38_001656"
     CDS             13844..15340
                     /locus_tag="ACKB38_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980389.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15340..16266
                     /gene="oppC"
                     /locus_tag="ACKB38_001657"
     CDS             15340..16266
                     /gene="oppC"
                     /locus_tag="ACKB38_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGL7980390.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16275..17342
                     /locus_tag="ACKB38_001658"
     CDS             16275..17342
                     /locus_tag="ACKB38_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980391.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            17353..18294
                     /locus_tag="ACKB38_001659"
     CDS             17353..18294
                     /locus_tag="ACKB38_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7980392.1"
                     /translation="MSEKLVEIKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFS
                     LVGESGSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPA
                     ASLNERATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQ
                     RQRIGIARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSV
                     VRFISDRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYD
                     PSQHDYETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5329 a         3300 c         3903 g         5800 t
ORIGIN      
        1 gcggtgtagc tcagctggct agagcgtccg gttcataccc gggaggtcgg gggttcgatc
       61 cccttcgccg ctataatgat cttgtcggac ctttagctca gctggttaga gctctcggct
      121 cataaccgag tggtcgtagg ttcaagtcct acaaggtcca tttgaatatt ggaggattac
      181 ccaagtccgg ctgaagggaa cggtcttgaa aaccgtcagg cgtgtaaaag cgtgcgtggg
      241 ttcgaatccc acatcctcct tttatattat taacgcggga tggagcagct cggtagctcg
      301 tcgggctcat aacccgaagg tcgtaggttc aaatcctgct cccgcaataa ggctcggtag
      361 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cggttcgatt ccgtctcgcg
      421 ccattttatt gatagtattt atgcgggtgt agtttagtgg taaaactaca gccttccaag
      481 ctgttgtcgc gagttcgatt ctcgtcaccc gctttgaact ttgttcaaat taccaagttt
      541 tttaacttgg gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt
      601 ggttcgagtc cactcgtgcc cattaattgg agaattactc aagaggctga agaggacggt
      661 ttgctaaatc gttaggtcgg gtaactggcg caagggttcg aatcccttat tctccgtttt
      721 aatgagagtc tgtaactctc ttttttattt ttagagccaa taaaacttca gatccgtaaa
      781 acttaattga attactaatc tgaagttatt tttatatcgt ttatattata attgtgttat
      841 tttacttcca actattgaga gaaatttcgc cactatttag ccagatttct tttccgttat
      901 cacattgaac taaaagtttt ccattttctg agatgtcttt agcaagtccc ttgtagtctt
      961 tttgctctag tgtgaaagtg acttcttttc ctagaatgaa tgactgtttt ttgtataggt
     1021 ataatagctc ttctgctggt gtttcgaaga aagcacgcca gatttctatg atcaattcat
     1081 tccttgttat aggagctgta gctttaaata agctggcagc tttttctttt aattcctgag
     1141 ggaagtcttt aatagtgaaa ttgataccta ctccaataat gatatctgtg actaagccag
     1201 tttctacaga ggtcattgct tcagtaagga ttcctccaat tttatgattg tttagataga
     1261 tatcattgac ccattttatg tcgacatcta ttaaagttag gttcttaatg gctttgtaga
     1321 cagctccagc tacaagtagt gtgtaggatg gtaatttgtc ataggggaga tttggtttaa
     1381 gatggagtgt catataaata ccaccttgtg gtgagtagaa ggaacgttga aaacggcctc
     1441 ggcctgctgt ttgataggaa gctagataga gggtatttgc ttcatggcct aaatcaattg
     1501 cttcttttgc atctagttgt gttgattttg tttcgggttt aaagctgact ttaattggaa
     1561 gattttcttc tagaatctct ggaagaataa ggtcaccatt catcagttta tatcctctat
     1621 ttttgatact atcaatttca atgccttctt gttctagtcg cttgatggct ttccaaattg
     1681 ctgttcggct tagggatagt ttttctgcga ttttttctcc gctgatatag tcggtttctt
     1741 tagataggat ttggtagaca gcttggtagg atttcataat gttgcctttc ttactagttg
     1801 gtattgagag tattcttttc ttgtatgact tggagtctga ttaaagtatt gtttataagc
     1861 tttcgaaaaa tggagtggat ctgaaaaacc taccgagtat gcaattacct tgatggactc
     1921 ttgggtattt tcgagaagtt gtctagctcg gtgcattcga acgtagagta ggtattcttt
     1981 gggtgataag gtattaaatt ctttgaatac gcttgataag tagcttctgt gaacggatag
     2041 ttcttttgct aaatcttgaa ttgtaagtga ttgaggatag tggctatcaa ttaatcgttt
     2101 gcattcaaga tagagttggt gggttgatga aatattcttt tttttctgat tgggagcaat
     2161 agttcccaga tgaaacatca gttcatgaag ttgtcccatg atatggagtt gagctaattc
     2221 acttgatttt gtaatctgag cgaagcggac aatgtctgag atgagttttg cagtagtctg
     2281 ggtatgacaa gtttcagatt ggatgagata ggattgatca gaaatttggg aaagagcaaa
     2341 ataatcaggg gctttccctc cagtgattcc taaccagtag taggcccaag gttctttact
     2401 atctgcttga taaaaggtta gttcctctgg ttttaataga aagaaatctc cttcttttaa
     2461 atcaacaatt ttacccttgt aatgaaattt tccttgtcct ttagtaatgt aatgtagaac
     2521 gtatgtatca cgaatggctg gaccaaaaga gtaattaggt gtgcattcct catatccata
     2581 aaagcttagg gcaaggtcga ttgttccagt ctggtattct gaaaaaacta acatgtgcta
     2641 cctcctacct aacattttac catattcttg agacattttt ctattttgga aagcgatttc
     2701 aggtgataaa atataatcaa taaagtgatg aggtgaagta aatgggagtt aggatagaga
     2761 ataatctatt ttatgttgag agtaaaaatc taagtttgat tattgaaaat cgaaatggct
     2821 acttactttt gaaacattta ggaaagacta ttaagaacta taaaggttcc aatagtgttt
     2881 atgaacgaga ccatgccttt tcaggaaatc caacggctac taatcgaacc tttagtttag
     2941 atactcagcg acagattttt ggacaacatg gcttaggaga ttttaggaaa ccaaccatac
     3001 aggttcagca tagtgtaact gaagtaacag actttcgatt tgtagaagca aagattttaa
     3061 aaggtcagaa tggtccacag ggcttacctt ctccacatag catggacgat acagagactc
     3121 ttgtcttaat gttagaagat tctaaggctc aacttagtct gactttgtat tatactactt
     3181 ttaataatga tgcgactatt gctagctaca gtaaattaga taataatagt aatcaggaag
     3241 ttgtcatcca taaagatttt tcttttatgg ctgattttcc agctgcagat tacgaaatag
     3301 taactctgca gggtgcttat gctcgtgaaa agactgttag acgtcaacag gtagaacaag
     3361 gaatcttttc gattagttca aaccgaggtg cttctggtca tgctcaaaca ccagctcttc
     3421 tactatgcga acaaggagtc acagaggatg ctgggaatgt gtttgctatt caactaatgt
     3481 atagtggcaa ctttgaagct tttgttcaaa aaaatcaatt gaatgaagtt cgggtggcta
     3541 ttggcattaa tccagaaaac ttttcttgga agttagctcc tgaggaatac tttgaaacac
     3601 cggtagcttt agtgactcat tcagatcagg gattaactgg tattagtcat gaaagtcaga
     3661 attttgtact gaagcacatt atgctaagtg aattttctaa aaaagaacgt ccaattctaa
     3721 tcaataactg ggaagctact tactttgact ttcagagaga aaaactgtta gagttagcag
     3781 atgaagctaa gaaagttggc attgaacttt ttgtattaga tgatggttgg tttggcaatc
     3841 gttttgatga taatcgtgct ttaggtgatt gggttgttaa tgaggaaaaa ctgggtggaa
     3901 gtctagaaag tctgatttca gctatccatg aaagaggttt gcagtttgga ctttggttag
     3961 aacccgaaat gatttctgta gatagtgatt tgtatcgtca acatcctgac tgggctattc
     4021 aggttcctgg ctatgagcat acttattctc ggaatcaatt agtacttaat cttgccaatc
     4081 ctcaggtagt agaatacttg aaaagtgtct tagatcaact cctattttat catgatattg
     4141 attacattaa atgggatatg aaccgcaata tcactaagct agggaatgga ttaacttatc
     4201 tagagacaca gatgcaatct catcagtaca tgctggggct ttatgaactc gtttcttatc
     4261 tgacagagaa gcacagccat attctctttg agtcctgctc tggtggtggt ggacgaaatg
     4321 atcttggtat gatgcgctat ttcccacaag tctgggctag tgataatact gatgccattg
     4381 cacgtttacc aattcaatac ggttcatcct atctctatcc aaccatttct atgggggctc
     4441 atgtgtcagc agtaccgaat catcagatgg gacgaatgac accattagaa acacgtggcc
     4501 ttgtagcaat gatgggaaat ttgggctatg aacttgattt gacaaattta tcagatgaag
     4561 agaaagctac gattgctaat caggtgaact tgtataaaga attacgacca gtagttcagt
     4621 taggacagca gtatagacta attaatcctg atactgtatc caatgaagca gctgtacaat
     4681 ttaattacgg aaatcaaacg attgtaacct acgttcgcgt tttatctgtt gtagagacca
     4741 tggaaacaac tttaaaatta aaagatttgg atgaagaggg actatataaa ttacaggaaa
     4801 atggcgaagt ttactcaggt gcagaactaa tgtatgcggg cttaactgtt atcttatccc
     4861 aaggagattt tttgagtaga cagtatattt ttagaaaact atgatatgta ctgaccccaa
     4921 aagttagatt tattctgtct aacttttggg gtgcagttca ataagggtgt ataaatttat
     4981 aaaggaggct ttctaatgga atggtataaa aaaatcggac ttcttgcaac tacaggttta
     5041 gctttgtttg ggctcggcgc ttgctccaac tatggtaaat ctgcggatgg cacagtgacc
     5101 atcgagtatt tcaaccagaa aaaagaaatg accaaaacct tggaagaaat cactcgtgat
     5161 tttgagaagg aaaaccctaa gatcaaggtc aaagtcgtca atgtaccaaa tgctggtgaa
     5221 gtattgaaga cacgcgttct cgcaggagat gtgcctgatg tggtcaatat ttacccacag
     5281 tccatcgaac tgcaagaatg ggcaaaagca ggtgtttttg aagatttgag caacaaagac
     5341 tacctgaaac gcgtgaaaaa tggctacgct gaaaaatatg ctgtaaacga aaaagtttac
     5401 aacgttcctt ttacagctaa tgcttatgga atttactaca acaaagataa attcgaagaa
     5461 ctgggcttga aggttcctga aacctgggat gaatttgaac agttagtcaa agatatcgtt
     5521 gctaaaggac aaacaccatt tggaattgca ggtgcagatg cttggacact caatggttac
     5581 aatcaattag cctttgcgac agcaacaggt ggaggaaaag aagcaaatca ataccttcgt
     5641 tattctcaac caaatgccat taaattgtcg gatccgatta tgaaagatga tatcaaggtc
     5701 atggacatcc ttcgcatcaa tggatctaag caaaagaact gggaaggtgc tggctatacc
     5761 gatgttatcg gagccttcgc acgtggggat gtcctcatga caccaaatgg gtcttgggcg
     5821 atcacagcga ttaatgaaca aaaaccgaac tttaagattg ggaccttcat gattccagga
     5881 aaagaaaaag gacaaagctt aaccgttggt gcgggagact tggcatggtc tatctcagcc
     5941 accaccaaac atccaaaaga agccaatgcc tttgtggaat atatgacccg tccagaagtc
     6001 atgcaaaaat actacgatgt ggacggatct ccaacagcga tcgaaggggt caaacaagca
     6061 ggagaagatt caccgcttgc tggtatgacc gaatatgcct ttacggatcg tcacttggtc
     6121 tggttgcaac aatactggac cagtgaagca gacttccata ccttgaccat gaactatgtc
     6181 ttgaccggtg ataaacaagg catggtcaat gatttgaatg ccttctttaa cccgatgaaa
     6241 gcggatgtgg attaggagta gagagactat gaaaaaagta ttacaaaaat attgggcatg
     6301 ggcttttgtg gtcatccccc tcttgttaca agcaattttc ttctatgtgc cgatgtttca
     6361 aggagccttt tacagtttta ccaactggac aggattgact tataactaca aatttgttgg
     6421 cttaaacaac tttaagctcc tcttcatgga tccaaaattc atgaatgcga ttggctttac
     6481 cgcaatcatt gcgattgcca tggtggttgg tgagattgca ctcgggatct tcattgcgcg
     6541 tgtcttgaat tctaaaatca aaggccaaac cttcttccgt gcttggttct tcttcccagc
     6601 tgttttatct ggtttgacag tggctttgat cttcaagcaa gtcttcaact acggtcttcc
     6661 agcgattgga aatgcccttc atattgaatt tttccaaacc agtcttttag ggactaagtg
     6721 gggagcaatc tttgcggctg tctttgtcct tctttggcaa ggggtggcta tgcccatcat
     6781 catcttccta gctggtttgc aatctattcc aactgagatt acagaggcag caaggattga
     6841 tggtgcgact agcaagcaag ttttctggaa cattgaattg ccttacttgc taccaagtgt
     6901 ctctatggtc tttatcctag ccctaaaagg tgggctgact gcctttgacc aagtctttgc
     6961 catgaccggt ggtggtccaa acaatgccac aacctcactt gggctcttgg tttataacta
     7021 tgcctttaaa aacaaccaat tcggttatgc caatgccatt gccgtaatct tgttcttctt
     7081 aattgtagtg atttcgatca tccaattgag agtatctaag aaatttgaaa tttaagagga
     7141 gaagcatgat gaaacaagat gaaagaaaag ccctgattgg caaatacatt ctattgattc
     7201 taggatcggt tctgatttta gtgccgctcc ttgctaccct ctttagttcc ttcaaaccca
     7261 ctaaggatat tgtagataat ttctttggct ttccaaccaa cttcacatgg gacaacttta
     7321 gccgtctctt agctgatggg attggaggct attattggaa ctctgtcgtc atcactgtct
     7381 tgtctttact tgcagtaatg atctttatcc ctatggcagc ctactccatc gctcgcaata
     7441 tgagtaaaag aaaagccttt accatcatgt ataccctctt aatcctcgga atcttcgtac
     7501 ctttccaagt catcatgatt ccgattacgg ttatgatgag taaactcggt ttggctaata
     7561 cctttggttt gatcttgctc tacttgacct atgcgattcc acagaccctc tttctctatg
     7621 ttggctatat caaaatctcg attccagaaa gtctggatga agcagcagag atcgatgggg
     7681 ctaatcaatt tacaacctat ttccgcatca tcttcccaat gatgaaaccg atgcatgcga
     7741 caaccatgat catcaatgcc ctttggttct ggaatgactt catgttgcca ctccttgtct
     7801 tgaaccggga ttccaaaatg tggactctgc ctttgttcca atacaactac gcaggccaat
     7861 atttcaacga ctacggacca agctttgcct cttacgtggt cggcattatc agtatcacca
     7921 ttgtctatct cttcttccaa cgccatatca tttcaggaat gagcaacggg gcagtgaagt
     7981 aatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc tcaaaacact
     8041 gttttgaggt tgtggatgaa actgacgaag tcagtaacca tacctacggc aaggtgaagc
     8101 tgacgtggtt tgaagagatt ttcgaagagt ataagaagag ctcatggaat ctatagtctt
     8161 tgacacagag gaagagttta gcttgttgat aattctatgc agaaaacaag aaagtagtac
     8221 tttgtttatc acagtctata ttagtacgag tttgatgata aatagagatt taaaatgaat
     8281 tttatttatg aagccagatc atatgagact tggcttcaat tagaaaaagg agagtaatga
     8341 tgccaattca aaataaaacc atgttgatta cctattctga tagccttgga aataatctta
     8401 aagacttata tgataatttg gaagagcatt ttggagatgc tattggagga gttcaccttt
     8461 taccattttt cccatcaaca ggtgatcgtg gatttgcgcc agttgactac gacgaagtgg
     8521 actcagcttt tggtgattgg gaggatgtga agcgtttagg tgagaaatat tatcttatgt
     8581 ttgattttat gattaatcat atttctcgtc aatccaagta ttataaggac tatcaagaaa
     8641 aacatgaagc cagtgaattt aaagctctct ttttaaactg ggataagttt tggccagaaa
     8701 accgtccgac acagtctgat gtagatttaa tttacaagcg taaggatcgt gcaccaaagc
     8761 aagagattgt gtttgaagat ggttcagtgg aacatttgtg gaataccttt ggtgaggagc
     8821 agattgatct tgatgtgacc aaagaagtaa ctatggaatt tatccgtaag accattcagc
     8881 acttggcaag taatgggtgt gatttgattc gtctagatgc ctttgcttat gcagtgaaga
     8941 aattggatac taatgatttc tttgtggaac cagatatttg ggatttattg gacaaagttc
     9001 gagatatcgc tgctgagtat gggacagagc ttttacctga gattcatgaa cactattcga
     9061 ttcagtttaa aatagcagac catgattact atgtttatga ttttgctctt ccaatggtga
     9121 cactttatac tctttacagt tccagaacag agcgtttggc taagtggtta aagatgagcc
     9181 cgatgaagca atttacgacg ctagataccc atgatgggat tggagtagta gatgtcaagg
     9241 atatcctgac cgatgaggag attgactatg cttcaaatga actctataag gttggagcca
     9301 atgtcaaacg taagtactct agtgccgagt ataacaactt agatatctac caaatcaatt
     9361 caacctacta ttcagcgctt ggagatgatg atgtcaagta ttttctcgct cgtctaattc
     9421 aagcttttgc cccaggtatt cctcagattt actatgtggg tctattagca ggcaagaatg
     9481 acttgaaatt attagaagaa actaaagaag gtcgaaatat taatcgtcat tactatagca
     9541 acgaggaaat agcaaaagaa gtgcaacgac ctgttgtgaa ggcccttctc aatctatttt
     9601 ctttccgtaa ccgttcagaa gcctttgatc tagaagggac tactgagata gagacaccaa
     9661 cagctcacag cattgtaatc aaacgtcaaa ataaagataa gtccgtaaca gcagtagtag
     9721 aaattgattt gcaaaatcag acttatcggg taattgagaa tggagttgaa gtatcatttt
     9781 gaagccttga tatggtggat aaaatagggt ttttattttg gaaaacgttt cctttgtttt
     9841 caaattgcta aaaaagtggt acaatatagg atagcttact attatctgaa tcagctgatt
     9901 tggagagaaa ggattcattt tgaaatcaat aggctttatt gaaaagctga aggggttgtc
     9961 tagtaaagag ctgattttat tgggaattat cctgagtatc tttttaccct tttatctttt
    10021 tgtagttgta ctctgtttat atattatcag tttgattttt acaggagaca tgaaaagtat
    10081 tcttcagaaa atgggggagc atccgatgct gcttcttttt cttagctata gtactgttat
    10141 atccattctt gcacaaaatt ggatgggtct tgtggcttca gtaggaatgt ttctatttac
    10201 tattttcttt ttgcactatc agtcgatttt atcccataaa ttctttcgat tgattttgca
    10261 gtttgtcttg tttggtagtg tcttgtcagc tgcttttgcc agtttagaac atttccaaat
    10321 tgtgaagaaa tttaactatg cttttctttc acccaatatg caggtgtggc atcagaaccg
    10381 ggcagaagtg accttcttta atcctaatta ttatggaatt atttgttgtt tctgtattat
    10441 gattgctttc tatctgttta caacgaccaa gttgaattgg ttgaaagtat tctgtgtgat
    10501 tgcaggcttt gttaatctct ttggtttgaa ctttactcaa aatcgaactg cctttcctgc
    10561 tattatcgct ggagcaatta tctatctctt tacgactatt aaaaactgga aggccttttg
    10621 gcttagtatt ggggtcttcg cgattggttt gagtttcctc ttttctagtg atttgggagt
    10681 tcgaatgggt actttagact cttctatgga agaacgcatt tctatctggg atgctgggat
    10741 ggccttgttt aagcaaaatc ctttttgggg tgaagggcca ttgacctata tgaactctta
    10801 tcctcggata catgctcctt atcatgaaca tgcccacagt ctttatattg atacgattct
    10861 gagttacgga attgtgggga ctattttatt agttttgtct tctgttgctc ctgttcgctt
    10921 gatgatggat atgagtcagg agtcggggaa acgtccgatt atcggccttt atctatcttt
    10981 ccttacagtg gttgctgtgc acggaatttt tgacttggct ctcttctgga ttcagtcagg
    11041 ctttattttc ttgctagtta tgtgcagtat tccattggag catcgaatgt tggtatcgga
    11101 catgacggat taagttttat aagtttgaaa tcttctacct tgggtaggag attttttata
    11161 cgatgggcat gtcgtgcatc tgaggtgtaa gtcctctgta gggctccact accggtgaat
    11221 acaatagcgc tccccaagcc tgactatcgt gaggtagcgg attaaaatgg tctggggata
    11281 gatcgtttta agtctgacgc aggaaataag aattgtcaga gaaagcaata gtgaaatcgt
    11341 aggctatgaa tagaaacgtg atagcgagga gcatatagtg gataaggtag cgagaaattt
    11401 taaagtataa aagatagtct taatctatat atgtaaatca caaaagtaat tgaattcggg
    11461 ataaggttgg tgtgaaaaga catagtaaac tgaatcttga atactagaat gatacttcta
    11521 caatattgtt ataatttaat ttgaattcca aaatcacatt gttccgtccg tatttcattt
    11581 tactgtatgt cttcctaaac tccaaagttt ctgcgtcaaa tttcctattt tcactgtagt
    11641 ctttgacact tcgaacgaca atttgaatca tatattcaaa aaaaatgaaa aatttgtgcc
    11701 aacaagccta aatattctga aaatttgttt gatttttaat ggataatgtg atataatggt
    11761 tgtcaatcta taattcacat aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc
    11821 tacagcaggt cttgttttat tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc
    11881 atctgattca tcagccccta aagcttatgg ctatgtttat acagcagacc cagaaacctt
    11941 ggactacctg atttcaagta aaaatagtac aacagtagtg acttcaaatg ggattgatgg
    12001 tttattcact aacgataatt acggtaatct tgctcctgca gttgcagagg attgggaagt
    12061 ctctaaggat ggtttgacct acacttataa gattcgtaaa ggggttaaat ggtttacctc
    12121 tgatggagaa gaatatgcag aggtgacggc taaagatttc gtgaacggtt taaaacacgc
    12181 agcagataaa aaatcagaag ctatgtattt agctgaaaat tcggttaaag gcttggcaga
    12241 ttatctatca ggaacttcaa cagatttttc aacagttggt gtcaaggcgg ttgatgatta
    12301 tacgttacaa tacactttga accagcctga accgttctgg aactctaagt tgacctattc
    12361 tattttctgg cctctgaatg aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc
    12421 aacagatccg acatccttgc tttataatgg tccattcttg ttgaaagggt tgactgcaaa
    12481 atcttctgta gagtttgtaa aaaatgagca atattgggat aaagaaaatg tccacctaga
    12541 tactatcaat ctagcttact atgatggatc agatcaggag tcgctagagc gtaacttcac
    12601 tagtggagct tatagttatg cccgtcttta ccctaccagc tccaactatt ctaaggttgc
    12661 agaagaatac aaggacaata tctattacac acaatcaggc tctgggattg ctggtctggg
    12721 tgtgaatatt gatcgccaaa gttacaacta tacttctaaa actacagatt cagagaaagt
    12781 agctactaag aaggcattgc ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga
    12841 tcgctcagct tactcagctc aaatcaatgg taaagatgga gcagctttag cagttcgtaa
    12901 tttatttgta aaaccagact ttgtttcagc tggtgagaag acctttggtg atttagtcgc
    12961 tgctcaactt cctgcttatg gtgatgagtg gaaaggtgtg aatttagctg atgggcagga
    13021 tggtttattc aatgctgaca aggccaaggc agagtttgcg aaagctaaga aagctttaga
    13081 agcagacggc gttcagtttc ctattcatct ggacgttcca gtagaccaag catcaaaaaa
    13141 ctacatatct cgtattcagt cctttaaaca atctgtagaa acagttcttg gtgttgaaaa
    13201 tgtcgttgtt gatattcaac aaatgacaag tgatgaattc cttaatatta cttactatgc
    13261 tgccaatgct tcatctgagg attgggatgt atcaggagga gtttcatggg ggccagacta
    13321 tcaagaccca tctacttacc tggatatttt aaaaacaact agcagtgaaa ctacaaaaac
    13381 atatttagga tttgataatc caaatagccc ttcagtagtt caagttggtt tgaaagaata
    13441 cgataaatta gttgatgaag ctgccagaga gacaagcgac ttgaatgtcc gttatgaaaa
    13501 atatgcagcg gctcaagcat ggttgacaga tagttcactc tttattcctg ctatggcttc
    13561 ttctggtgca gcaccagtgc tttcacgaat tgttccattt actggagctt ctgcgcaaac
    13621 aggctctaag gggtcagatg tttacttcaa atatttgaaa ttacaagata aagtggtgac
    13681 taaggaagag tatgaaaaag ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga
    13741 gaaagctcaa aaagaattgg caagtcatgt gaagtaaata atttctaata gaactttctc
    13801 tccatgagga gaaggttctt ttgggatttt taaaggaaat aatatgaaaa aatatatttt
    13861 tatgcgtgtt ttgcggtcat tggtttcgat tttcttagta acgactttga cctacacgat
    13921 tatctatacc ttggttcctc gaaaattgat tttcaagcag gatcctaact ataataaaat
    13981 tgcgacaacg gctgataaac gtgataacta tgaaaatact gtgtttgagc gtatgggcta
    14041 cattgagtat tacgatacta aagagttgca agaaaaggca agtagcatgg attcttctgt
    14101 aacagtagaa gcaaatgcga ccaataaagc tatttatgaa aagtacatca atcaattagg
    14161 tcatggttgg actttgggag aatttactga aagtggtcaa ttctatgcta ctcgtgaaat
    14221 tccaattttt gaacgtgttt ttcacttcta tgctaacttg attgacattg accatacaaa
    14281 taaaatccaa gaccctgaaa atccagactt gaaacgctac cttcgttttg aaaatgatcc
    14341 agctatcgga tggtcattgg tcggttcagg aactaaacat aaatatctct tgtactttaa
    14401 cagtcagttc ccatttgttc atcaaaactt tgtgaacttg aatttaggtg actcttaccc
    14461 aacctatgct aatacaccag ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac
    14521 tgcccaagtt cagttcccaa caggtaagaa aacgtcttct gtaaatattt actcaagaac
    14581 ctacaagtca cctagtcagg ctgactctcg tgaagtagct agctatggga aagatgatcc
    14641 ttatacagcg actgaaagta attaccaata tccatctatg attgtcagct ctgctattac
    14701 tggtttgatt ggtttggttc ttgcctatgc tcttgccgtg ccacttggtt cagccatggc
    14761 tcgtttcaag aacacttgga ttgatagcct ctcaacaggg gctttgacct tcttgcttgc
    14821 tcttccaacg attgccttgg tttacatcgt tcgattgatt ggatcatcta ttgcccttcc
    14881 agattcattc cctatcttgg gagctggaga ttggcgttct tacgttttac cagcagtcat
    14941 ccttggtttg ttgggtgctc ctggtacagc catttggatt cgtcgttaca tgattgactt
    15001 gcaatctcaa gactttgttc gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc
    15061 aaacaaacac atctttaaaa atgccatggt tccgctggtt tcaggaattc ctgctgccat
    15121 tattggggtt atcggtggtg caacccttac tgaaacagtc ttcgccttcc caggtatggg
    15181 taaaatgttg attgactctg taaaagcatc taataactct atggtcgttg gtcttgtctt
    15241 catctttaca tgtatttcta tcttctcacg tcttttggga gatatttgga tgactattat
    15301 tgacccacgt attaaattga ctgagaaagg aggcaaataa tgtctacaat cgataaagaa
    15361 aaatttcagt ttgtaaaacg tgacgatttt gcctctgaaa ctattgatgc gccagcatat
    15421 tcttactgga aatcagtgtt taaacaattt atgaagaaaa aatcaactgt agtcatgttg
    15481 ggaatcttgg tagccatcat tttgataagt ttcatctacc caatgttttc taagtttgat
    15541 ttcaatgatg tcagcaaggt aaacgacttt agtgttcgtt atatcaagcc aaatgcggag
    15601 cattggttcg gtactgacag taacggtaaa tcgctctttg acggtgtctg gttcggagct
    15661 cgtaactcca tcctcatttc tgtgattgcg acagtgatta acttggttat cggtgttttt
    15721 gtcggtggta tttggggtat ttcaaaatca gttgaccgtg tcatgatgga agtttacaac
    15781 gtcatctcaa acatcccacc tcttttgatt gttattgtct tgacttactc aatcggagct
    15841 ggattctgga atctgatttt tgccatgagc gtaacaacat ggattggtat tgccttcatg
    15901 atccgtgtgc aaatcttgcg ctatcgtgac ttggaataca acttggcgtc acgtactttg
    15961 ggaacaccaa ccttgaagat tgttgccaaa aatatcatgc ctcaattggt atctgttatt
    16021 gtgacaacca tgactcaaat gcttccaagc tttatctcat acgaagcctt cttgtctttc
    16081 ttcggtcttg gattaccgat tacagtgcca agtttgggtc gtttgatttc ggattattca
    16141 caaaacgtaa caaccaatgc ttacttgttc tggattccat tgacaaccct tgtcttggta
    16201 tccttgtccc ttttcgtagt tggtcaaaac ttagcggatg ctagtgatcc acgtacacat
    16261 agataggagt agaaatgaca aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg
    16321 tggaatttga cgttcgtgac aaagtattga cagccattcg cggcgtttcc cttgaactag
    16381 tcgaaggaga agtattagcc ttggtaggtg agtcaggatc aggtaaatct gttttgacaa
    16441 agaccttcac aggtatgctc gaagaaaatg gtcgtattgc ccaaggtagt attgactacc
    16501 gtggtcagga cttgacagct ttatcttctc acaaggattg ggaacaaatt cgtggtgcta
    16561 agattgcgac tatcttccag gacccaatga ctagtttgga ccccattaaa acaattggta
    16621 gtcagattac agaagttatt gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat
    16681 tggccattga ctacatgaat aaggttggca ttccagacgc agatagacgt tttaatgaat
    16741 acccattcca atattctgga ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct
    16801 gccgacctga tgtcttgatc tgtgatgagc caacaactgc cttggatgta actattcaag
    16861 ctcagattat tgatttgcta aaatctttac aaaacgagta tcatttcaca acaatcttta
    16921 ttacccacga ccttggtgtg gtggcaagta ttgcggataa ggtagcggtt atgtatgcag
    16981 gagaaatcgt tgagtatgga acggttgagg aagtcttcta tgaccctcgc catccatata
    17041 catggagtct cttgtctagc ttgcctcagc ttgctgatga taaaggggat ctttactcaa
    17101 tcccaggaac acctccgtca ctttatactg acctgaaagg ggatgctttt gccttgcgtt
    17161 ctgactacgc aatgcagatt gacttcgaac aaaaagctcc tcaattctca gtatcagaga
    17221 cacattgggc taaaacttgg cttcttcatg aggatgctcc gaaagtagaa aaaccagctg
    17281 tgattgcaaa tctccatgat aagatccgtg aaaaaatggg atttgcccat ctggctgact
    17341 aggaggaagg aaatgtctga aaaattagta gaaatcaaat tagtagaaat caaagattta
    17401 gaaatttcct tcggtgaagg aagtaagaag tttgtcgcgg ttaaaaatgc taacttcttt
    17461 atcaacaagg gagaaacttt ctcgcttgta ggtgagtccg gtagtgggaa aacaactatt
    17521 ggtcgtgcta tcatcggtct aaatgataca agtaatggag atatcatttt tgatggtcaa
    17581 aagattaatg gtaagaaatc gcgtgaacaa gctgcggaat tgattcgtcg aatccagatg
    17641 attttccaag accctgccgc aagtttgaat gaacgtgcga ctgttgatta tattatttct
    17701 gaaggtcttt acaatcaccg tttatttaag gatgaagaag aacgtaaaga gaaagttcaa
    17761 aatattatcc gtgaagtagg tcttcttgct gagcacttga ctcgttaccc tcatgaattc
    17821 tcaggcggtc aacgtcaacg tatcggtatt gcccgtgcct tggtcatgca accagacttt
    17881 gttattgcag atgagccaat ttcagccttg gacgtttctg tacgtgccca agtcttgaac
    17941 ttgctcaaaa aattccaaaa agagctcggc ctgacctatc tcttcatcgc ccatgacttg
    18001 tcggttgttc gctttatttc agatcgtatc gcagttattt acaagggtgt tattgtagag
    18061 gttgcagaaa cagaagaatt gtttaacaat ccaattcacc catatactca agccttgctt
    18121 tcagcggtac caatcccaga tccaatcttg gaacgtaaga aggtcttgaa ggtttacgat
    18181 ccaagtcaac acgactatga gactgataag ccatctatgg tagaaatccg tccaggtcac
    18241 tatgtttggg cgaaccaagc cgaattggca cgttatcaaa aaggactaaa ctagtaatgg
    18301 ttttataatt tccatgtcaa tttttatgga aa
//
LOCUS       DBDESG010000051        17607 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.051, whole genome shotgun sequence.
ACCESSION   DBDESG010000051 DBDESG010000000
VERSION     DBDESG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.051"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            224..1669
                     /gene="cps4A"
                     /locus_tag="ACKB38_001660"
     CDS             224..1669
                     /gene="cps4A"
                     /locus_tag="ACKB38_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGL7980393.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTNNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKSQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRHQQKVIVAILQKLTST
                     EVLKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1671..2402
                     /gene="cps4B"
                     /locus_tag="ACKB38_001661"
     CDS             1671..2402
                     /gene="cps4B"
                     /locus_tag="ACKB38_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGL7980394.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            2411..3103
                     /gene="cpsC"
                     /locus_tag="ACKB38_001662"
     CDS             2411..3103
                     /gene="cpsC"
                     /locus_tag="ACKB38_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HGL7980395.1"
                     /translation="MKEQNTIEIDVFQLVKSLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPVDTRIVSISVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLHLELLDTRVKRPEDIENTLQMT
                     LLGVVPNLGKLK"
     gene            3113..3796
                     /gene="cps4D"
                     /locus_tag="ACKB38_001663"
     CDS             3113..3796
                     /gene="cps4D"
                     /locus_tag="ACKB38_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4D"
                     /protein_id="HGL7980396.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSINIAWSFARAGYKTLLIDGDTRNSVMLGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIIDTPPIGIVI
                     DAAIITQKCDASILVTATGEANKRDIQKAKQQLKQTGKLFLGVVLNKLDISVNKYGVY
                     GSYGNYGKK"
     gene            4137..4772
                     /gene="cps4E"
                     /locus_tag="ACKB38_001664"
     CDS             4137..4772
                     /gene="cps4E"
                     /locus_tag="ACKB38_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4E"
                     /protein_id="HGL7980397.1"
                     /translation="MKNGNRIYSWRLFMYGIIKRLGDILLSLIGIIILCPVFMIIAIA
                     IKLDSEGPVIFKQKRFGIHKEYFYILKFRSMKIDAPKNVAPRNLYNPEQWITKVGAFL
                     RKTSLDELPQLFNILVGNMSIVGPRPAGINELDLIAERDKYGANDILPGLTGWAQING
                     RDTLSVEMKTELDGYYVKHLSLIMDIRCIVKTIPYVLKRKGIVEGSGKKES"
     gene            4781..6010
                     /gene="cps4F"
                     /locus_tag="ACKB38_001665"
     CDS             4781..6010
                     /gene="cps4F"
                     /locus_tag="ACKB38_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4F"
                     /protein_id="HGL7980398.1"
                     /translation="MKILFVCQHYKPEPFRLSDICEDLVRKGHEVSVLAGIPNYPEGK
                     IYADYRHNKKRREIIEGVTIYRSYTIPRKKSVVFRLLNYFSFAISSTLGVLLGRYKTK
                     DGSNFDCVFVNQLSPVMMAWAGMAYKKKYKKPMFLYCMDVWPDSLTVGGVKQDGLIFK
                     LFKFISKKVYRASDYIFVTSPSFKNYFVKQFDISEQKITYLPQYAEDLFIPDESIVNK
                     ESVDLTFAGNIGKAQNLETILKAASLIEKNTNLPKKIHFHFVGDGTELLSMKALAHEL
                     ELKNISFYGRRSLEEMPSFYKKSDAMLVSLIGDSIVSRTIPGKVQSYMAAGKPIIGAI
                     SGDAKIIVEEANCGYVSPERDVKQLAKNICKFSMLSIKRQRELGKKARCYYENHFSKE
                     QFMLELETCLERESKKE"
     gene            6015..7091
                     /gene="cps4G"
                     /locus_tag="ACKB38_001666"
     CDS             6015..7091
                     /gene="cps4G"
                     /locus_tag="ACKB38_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4G"
                     /protein_id="HGL7980399.1"
                     /translation="MRVLFILSDNIYLTPYFNFYKELLKKLSISYDVIYWDKNINEII
                     TKQNYYRISFSGKGKLSKILGYVKFRKEIKKKLKENDYDMILPLHSIVSFILVDFLLF
                     SFKNRYIYDIRDYSYEKFLVYRLVQKQLVKNSLMNIVSSDGYKFFLPMGEYFTTHNLP
                     NMIELNEVKQLKNNSTFPIQLSYIGLIRFQEQNKKIIDFFANDSRFQLNFIGTNAGEL
                     REFCQEKNISNVNLVDTFQPKDTMSFYKNTDVVLNLYGNHTPLLDYALSNKLYFAALL
                     YKPILVCEDTYMEKVSIENGFGFVLPMKDESEKDCLALYIQNLDRKQLIKNCDNFMDR
                     ISLEKQKTEIELEKRILSLRKKND"
     gene            7084..8202
                     /gene="cps4H"
                     /locus_tag="ACKB38_001667"
     CDS             7084..8202
                     /gene="cps4H"
                     /locus_tag="ACKB38_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4H"
                     /protein_id="HGL7980400.1"
                     /translation="MIKVLHLFTTLDSGGVESFLFNYYSHIDRKKIQFDFIVPGKEQG
                     FLEDKMKELGAKVYHVPLLRKKPLHQFLSLARIIKKGDYDIVHCHGYKSAIGLILSKI
                     IGCKIRIIHSHMAYVTENSFQKVLRKLVTILVKILATHWFACGEDSAKWLYGEKAYKD
                     GKIEIIFNAIDLKKYQFLSDVREKCRRELDVSNKFVLGNIARLSDQKNQSYLFNVLKE
                     LILIKPNVILLLVGNGEDEQKLKQKALELNLTPYVLFLGRRTDISDLLSAMDVFLLPS
                     KYEGLPVSLVEAQASGLQILSSDTVTQEVDVTKNISYLPINEESVLLWKDKVLSLTSE
                     ECNRFEINNSMTDGLYDICYQASKLLNRYQEMCVIKEI"
     gene            8203..9603
                     /gene="wzy"
                     /locus_tag="ACKB38_001668"
     CDS             8203..9603
                     /gene="wzy"
                     /locus_tag="ACKB38_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HGL7980401.1"
                     /translation="MQTKYICRVTLVTLSFIFAFCYLFWTLDNWNNGFLISNYVPSIF
                     IWVCFLIIFQITGFILQKVSIYDFSVWYLILSYFFMFGLIFNEYMGFQTTLLWSPSNF
                     YNNEELFHSYIFIIWILFCYSVGYLFFYSDGKVHYHSEVQNYQENEEKILYNAGRILT
                     GVGFISRVITDSKTVLAVRAANSYSAYSEAASSGIIDDLGVLMLPGVFSLFYSDKLSR
                     VIKRTIFWVMLFYLILIMILTGSRKIQVFSILALVLVYTQSLGITFSKKRVLVFLIVT
                     VFLLNVLVVIRGHRFDLNTIGIYLFDSFSSLDFVKNILGEVFSESGLTSLTVASAVTV
                     VPSSIPYEYGMTFLRTILSIFPIGWLVGDFFDKASATVVINKFLGLPVGSSFVEELFW
                     NFGYYGGVFWSFVLGIFSGWRLNFRAFQTSKISKVIYFSVISQLLLLVRSSSIDVYRP
                     IMYSLIMIFIFRRLKK"
     gene            9606..10685
                     /locus_tag="ACKB38_001669"
     CDS             9606..10685
                     /locus_tag="ACKB38_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HGL7980402.1"
                     /translation="MKKIMLHGATDYGSSNYGDYLYGEIVYDLLESKGYEVSFYNPSD
                     FFQMYLKEYRQKQSFTKKQADAILYIPGGYFGEGHNARFRDNLIQFKRFLPLGIWASY
                     FKKPIGVLGIGAGPNNDSLMNYGIKRIINHAQFITVRDRESFDSLKHLSPSAPVHETF
                     DLIISSKLREEKTEQLCQLKREAKDKKIILVHYNHSKKALEKFAESISLFLENNPNYY
                     VVVTSDSILPYEDAYYQEFRKLVRTEDCFQFKYHSPAEMTSLLKMVDVVLTCKLHVGV
                     VATCFNKSVIAIACHPEKTARYYGAIGELQRCESLFDSSVNSIVKKLETFHLKPITIP
                     SELVLKARSSLDYLDLFLEGLVRES"
     gene            10675..11916
                     /locus_tag="ACKB38_001670"
     CDS             10675..11916
                     /locus_tag="ACKB38_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGL7980403.1"
                     /translation="MKVDRISFIKNTSSLYILNIVKLLFPLLTLPYLTRVLSLDAYGM
                     VIYVKALIAYVQLVIDFGFMISATKNIVNACTTPSKIGRIVGDTLVEKIFLSIISILI
                     YTILMWQIPIMRENILFSVFYLLATVTNIFIFDFLFRGIEKMHAVAIPYIISKTIITI
                     LTFIVVKDDSSILWIPILEGIGNLVAAVVSYRFLHYYGIKLSFSYLSVWVKDLKESSI
                     YFLSNFATTIFGVFTTVISGFYLQSQEIAFWGIAMQLLSAAKSLYNPIANSLYPHMIR
                     TKDIQSVKSINRIMFIPIIFGVLIVLFFSNQILSIIGGEKYTVSADFLKYLLPAFVAS
                     FYSMIYGWPVLGAIDKVKETTMTTILASIVQTLGLGIFILSDNFSLVTLAICSSMSEV
                     VLWISRYLIYFKNRSLFVRSK"
     gene            11921..13018
                     /gene="cps4I"
                     /locus_tag="ACKB38_001671"
     CDS             11921..13018
                     /gene="cps4I"
                     /locus_tag="ACKB38_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4I"
                     /protein_id="HGL7980404.1"
                     /translation="MKKVVVVFGTRPEAIKMCPLVKELRTRKNIETLVCVTGQHRQML
                     DQVLDTFGIIPDFDLSIMKDKQTLFDVTIGILEGMKAILESEKPDLVLVHGDTSTTFA
                     SSLAAFYLQIPIGHVEAGLRTYDIYSPYPEEFNRQAVGVLAQYHFTPTQLSKDNLLRE
                     GKTPESIFVTGNTAIDALQTTIQEDYTHPELEWIGESRFILITAHRRENLGEPMRHMF
                     RAIRRIIEEYSDVKAIYPIHMNPRVRQIAEEELSGCERIKMIEPLEVLDFHNFLSRSY
                     LILTDSGGIQEEAPSLGKPVLVMRDTTERPEGIEAGTLKLVGADENNIYRHFKELLEN
                     DSVYQAMSQASNPYGDGTACKKIADILEGEV"
     gene            13022..14077
                     /gene="cps4J"
                     /locus_tag="ACKB38_001672"
     CDS             13022..14077
                     /gene="cps4J"
                     /locus_tag="ACKB38_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4J"
                     /protein_id="HGL7980405.1"
                     /translation="MSQFTGKTLLITGGTGSFGNAVLKRFLETDVSEIRIFSRDEKKQ
                     DDMRHEFQVKVPEVAGKIRFYLGDVRDLASVKNAMHGVDYVFHAAALKQVPSCEFFPV
                     EAVKTNILGTENVLTAAIEAGVKQVICLSTDKAAYPVNAMGTSKAMMEKIAVAKSRTV
                     NPEHTKICVTRYGNVLCSRGSVVPLWIEQIKQGNALTITEPSMTRFVMTLEEAVDLVL
                     FAFEEGKSGDILVQKAPACTIEVLAKAVSEIFASEQDIKIIGIRHGEKRYETLLTNEE
                     CANAIDLGDFYRVPSDNRNLNYDKYFKDGSTNRNLLTEFNSNNTDLMDVEQVKRKLLE
                     LDEIQTAIRDMVADEEM"
     gene            14177..15406
                     /gene="cps4K"
                     /locus_tag="ACKB38_001673"
     CDS             14177..15406
                     /gene="cps4K"
                     /locus_tag="ACKB38_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4K"
                     /protein_id="HGL7980406.1"
                     /translation="MIKNILITGAKGFVGKNLICTLEALKDGRDRTRPNLEIGEIFQY
                     DRDTDPILLDEYCKKADFVFHLAGVNRPQNPDEFMEGNYGFSSRLLEILEKYENTCPV
                     LLSSSTQASLEGRFSNSIYGQSKLAGEELFFEYGKKTGAPVLVYRFPNLYGKWCRPNY
                     NSAVATFCYNLAHDLPIQVNDPSVELELLYIDDLIQECLTALEGNPHRCNLDGLQILP
                     SPSGNYCYVPTTHRATLGEIVSLLETFKKQPDSLVMPEIPQGSFKKKLYSTYLSYLPV
                     DKFKFPLKMNIDERGSFTELLKTENTGQFSVNISKPGITKGQHWHHSKWEFFMVVSGR
                     ALIQERRIGLDENGQEYPILNFEVSGDKIEAIHMIPGYAHNIINLSDTENLITVMWAN
                     ESFDPRHPDTFFEQVEK"
     gene            15407..16591
                     /gene="cps4L"
                     /locus_tag="ACKB38_001674"
     CDS             15407..16591
                     /gene="cps4L"
                     /locus_tag="ACKB38_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4L"
                     /protein_id="HGL7980407.1"
                     /translation="MKIKTDYSDIHFKDNGKLKLLIIVGTRPEIIRLSSVITKCRKYF
                     DVILAHTGQNYDYNLNGIFFDNLGLDTPDVYMDAVGDDLGATVGNIINTSYKLMNQIK
                     PDALLILGDTNSCLSAIAAKRLHIPIFHMEAGNRCKDECLPEETNRRIVDVISDVNLA
                     YSEHARKYLHECGLPKERTYVTGSPMAEVLHKNLSAIESSDIHERLGLKKGGYILLSA
                     HREENIDTDKNFISLFTAINQLAEKYNMPILYSCHPRSKKRLQESGFKLDKRVIQHEP
                     LGFHDYNCLQMNAFVVVSDSGTLPEESSFFTSQGYPFPAVCIRTSTERPESLDKAGFI
                     LAGIDENSLLQAVETAVSLAQDEDFGLPVPDYVEENVSTKVVKIIQSYTGIVDKIVWR
                     KS"
     gene            complement(17182..17607)
                     /locus_tag="ACKB38_001675"
                     /pseudo
     CDS             complement(17182..17607)
                     /locus_tag="ACKB38_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5716 a         2512 c         3515 g         5864 t
ORIGIN      
        1 ttagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgaa cgatttgttc tgttcttatt tcattttact atatttttgt ttcgcgggaa
      121 gtctactaag atacttaaag atgcagatag taaaaataaa ggtgtagaca ttaccgtaaa
      181 aaagtgatat aatcgtataa tgttcaatgt ataggtatta atcatgagta gacgttttaa
      241 aaaatcacgt tcacagaaag tgaagcgaag tgttaatata gttttgctga ctatttattt
      301 attgttagtt tgttttttat tgttcttaat ctttaagtac aatatccttg cttttagata
      361 tcttaatcta gtggtaactg cgttagtcct actagttgcc ttggtagggc tactcttgat
      421 tatctataaa aaagctgaaa agtttactat ttttctgttg gtgttctcta tccttgtcag
      481 ctctgtgtcg ctctttgcag tacagcagtt tgttggactg accaatcgtt taaatgcgac
      541 ttctaattac tcagaatatt caatcagtgt cgctgtttta gcagatagtg agatcgaaaa
      601 tgttacgcaa ctgacgagtg tgacagcacc gactgggact aataatgaaa atattcagaa
      661 attactagct gatatcaagt caagtcagaa taccgatttg acggtcaacc agagttcgtc
      721 ttacttggca gcttacaaga gtttgattgc aggggagact aaggccattg tcctaaatag
      781 tgtctttgaa aacatcatcg agtcagagta tccagactac gcatcgaaga taaaaaagat
      841 ttatactaag ggattcacta aaaaagtaga agctcctaag acgtctaaga gtcagtcttt
      901 caatatctat gttagtggaa ttgacaccta tggtcctatt agttcggtgt cgcgatcaga
      961 tgtcaacatc ctgatgactg tcaatcgaga taccaagaaa atcctcttga ccacaacgcc
     1021 acgtgatgcc tatgtaccaa tcgcagatgg tggaaataat caaaaagata aattgactca
     1081 tgcgggcatt tatggagttg attcgtccat tcacacctta gaaaatctct atggagtgga
     1141 tatcaattac tatgtgcgat tgaacttcac ttcgtttttg aaattgattg atttgttggg
     1201 tggaattgat gtttataatg atcaagaatt tactgcccat acgaatggaa agtattaccc
     1261 tgcaggcaat gttcatcttg attcagaaca ggctctcggt tttgttcgtg agcgctactc
     1321 cctagcagat ggcgatcgtg accgcgggcg ccatcaacaa aaggtgattg tggctatcct
     1381 tcaaaaatta acgtcaaccg aagtgctgaa aaattatagt acgatcatta atagcttgca
     1441 agattctatc caaacaaata tgccacttga gaccatgata aatttggtca atgctcagtt
     1501 agaaagtgga gggaattata aagtaaattc tcaagattta aaagggacag gtcggatgga
     1561 tcttccttct tatgcaatgc cagacagtaa cctctatgtg atggaaatag atgatagtag
     1621 tttagctgta gttaaagcag ctatacagga tgtgatggag ggtagatgaa atgatagaca
     1681 tccattcgca tatcgttttt gatgtagatg acggtcccaa gtcaagagag gaaagcaagg
     1741 ctctcttggc agaatcctac agacaggggg tgcgaaccat tgtttctacc tctcaccgtc
     1801 gcaagggcat gtttgaaact ccggaagaga agatagcaga aaactttctt caggttcggg
     1861 aaatagctaa ggaagtggcg agtgacttgg tcattgctta cggggctgaa atttattaca
     1921 caccagatgt tctggataag ctggaaaaaa agcggattcc gaccctcaat gatagtcgtt
     1981 atgccttgat agagtttagt atgaacactc cttatcgcga tattcatagc gccttgagca
     2041 agatcttgat gttgggaatt actccagtca ttgcccacat tgagcgctat gatgctcttg
     2101 aaaataatga aaaacgcgtt cgagaactga tcgatatggg ctgttacacg caagtaaata
     2161 gttcacatgt cctcaaaccc aaactttttg gcgaacgtta taaattcatg aaaaaaagag
     2221 ctcagtattt tttagagcag gatttggttc atgtcattgc aagtgatatg cacaatctag
     2281 acggtagacc tcctcatatg gcagaagcat atgaccttgt tacccaaaaa tacggagaag
     2341 cgaaggctca ggaacttttt atagacaatc ctcgaaaaat tgtaatggat caactaattt
     2401 aggagaaatg atgaaagaac aaaatacgat agaaatcgat gtatttcaat tagttaaaag
     2461 cttgtggaaa cgcaagctaa tgattttaat agtggcactt gtgacaggtg cgggggcttt
     2521 tgcatatagc acttttattg ttaagccaga atatacgagt accacgcgaa tttacgtagt
     2581 gaatcgcaat caaggagaca agccggggtt gacaaatcag gatttgcagg caggaactta
     2641 tctggtaaaa gactaccgtg agattatcct ttcgcaggat gttttggagg aagttgtttc
     2701 tgatttgaaa ctagatttga cgccaaaagg tttggctaat aaaattaaag tgacagtacc
     2761 agttgatacc cgtattgtct ctatttcagt taatgatcga gttcctgaag aggcaagccg
     2821 tatcgctaac tctttgagag aagtagctgc tcaaaaaatt atcagtatta ctcgtgtttc
     2881 tgacgtgaca acactggagg aggcaaggcc ggcgatatcc ccgtcttcgc caaatattaa
     2941 acgcaataca ctaattggtt ttttggcagg ggtgattgga actagtgtta tagttcttca
     3001 tcttgaactt ttggatactc gtgtgaaacg tccggaagat atcgaaaata cattgcagat
     3061 gacacttttg ggagttgtgc caaacttggg taagttgaaa taggagagaa ggatgccgac
     3121 attagaaata gcacaaaaaa aactggagtt cattaagaag gcagaagaat attacaatgc
     3181 cttgtgtaca aatatacagt tgagcggaga taaactaaaa gtaatttccg ttacttctgt
     3241 taaccctggg gaaggaaaaa caactacttc cataaatata gcatggtcgt ttgcgcgtgc
     3301 aggctataaa actcttttga tcgatggcga tactcgaaat tcagttatgt taggagtttt
     3361 taaatctcgt gaaaaaatta cagggctaac agaattttta tctgggacag ctgatttatc
     3421 tcacggttta tgtgatacaa atattgaaaa tttatttgta gttcaatcgg gatctgtatc
     3481 accaaaccct acagccttgt tacaaagtaa aaattttaat gatatgattg aaacattgcg
     3541 taaatatttt gattatatca ttattgatac accgcctatt ggaattgtta ttgatgcggc
     3601 aattatcact caaaagtgtg atgcgtccat cttggtaaca gcaacaggtg aggcgaataa
     3661 acgtgatatc caaaaagcga aacaacaatt aaaacaaaca gggaaactgt tcctaggagt
     3721 tgttttaaat aaattggata tctcggttaa taagtatgga gtttacggtt cctatggaaa
     3781 ttatggtaaa aaataaaatg aatatagtat ggaaggggac tacaatataa ctctggcatt
     3841 cttacagagc atacttatca gtttattagc ttatgtactg attgcaattt cagaaaccga
     3901 tatgtcttca aataatattc ggtctatttg gaagaataat tgaactggac cttctaattc
     3961 attattgaga actcatatat tactgaaaca aataaatttt gtttagatgg agaaaagttt
     4021 tatctgacgt agaggataaa agaattatat ttctgaaaat cattgagtga ccaaatatat
     4081 attgagtttc tgtagttgca tgtactgcct gatttaattt gtcaaatgga aatagaatga
     4141 aaaatggaaa tagaatttat agttggaggt tgtttatgta cggtataata aaacgattag
     4201 gtgatatatt attatcttta atagggataa taatattgtg tccggttttt atgataattg
     4261 caattgcgat taaacttgat tcagaaggtc cggttatatt taagcaaaaa cgctttggta
     4321 ttcataaaga atacttctat attttgaaat ttaggtctat gaaaatagat gcacctaaaa
     4381 atgtggcgcc tcgaaactta tataatccag agcaatggat tacaaaagta ggggctttct
     4441 tgcgaaaaac atctttggat gaactaccac aattgtttaa tattcttgtt ggtaatatga
     4501 gtattgtagg tcctagacca gcgggtataa atgaactaga tttgattgca gagagagata
     4561 agtatggagc aaatgatatc ttgccagggt taactggatg ggcacaaatt aacgggcgtg
     4621 atactttgtc tgttgagatg aagacggagt tagatggcta ctatgttaaa catctgtctt
     4681 tgataatgga tattagatgt atagttaaga caatacctta cgtactgaaa cgaaaaggta
     4741 ttgtagaggg tagtggtaag aaagaaagtt aaattggaca atgaaaatac tatttgtttg
     4801 ccaacattat aagccagaac cattcaggtt gtcagatatt tgtgaagatt tagttcgaaa
     4861 agggcatgaa gtctctgttt tggctgggat tcctaattac cctgaaggga agatatatgc
     4921 agattatcgt cataataaaa aaagacgtga gattatagaa ggtgttacga tatatcgttc
     4981 ttatacaatc cctagaaaaa aaagtgttgt atttcgattg ttgaattatt ttagctttgc
     5041 aattagttct actttaggag ttttattggg gaggtataaa acgaaagatg gatcgaattt
     5101 tgactgtgta ttcgttaacc aattgtctcc agttatgatg gcatgggctg gtatggctta
     5161 taaaaaaaaa tataagaaac cgatgtttct atattgtatg gatgtttggc cagatagttt
     5221 aaccgtaggt ggagtgaaac aagatggctt gattttcaag ctgtttaaat ttatctcaaa
     5281 aaaagtttac cgagctagtg attatatatt tgtcactagt ccatcattta aaaattattt
     5341 tgtgaagcaa tttgacatat ccgaacaaaa gattacatat ttgccacaat atgcagaaga
     5401 tctttttatc cctgatgaat ctatagttaa taaagaaagt gttgacctaa cttttgctgg
     5461 taatattggc aaagcacaaa atttggaaac tattttgaaa gctgccagtt tgatagagaa
     5521 gaataccaat ttacccaaga aaattcattt tcattttgtt ggagatggta cggaattgtt
     5581 aagcatgaaa gcattagctc atgaattgga gttaaagaat atttccttct atggaagacg
     5641 ttctttggag gaaatgccat ccttctataa aaaatcagat gctatgttag tttctttaat
     5701 aggagactcg atagtttctc gtactatacc tgggaaggta caatcttata tggcggcagg
     5761 caaaccaatt ataggtgcaa tttcaggaga tgctaaaata attgtagaag aagcaaattg
     5821 tggatatgtt agtcccgaac gagatgtaaa acaattggca aaaaatattt gtaaatttag
     5881 tatgttatct attaagagac aaagagagtt aggaaagaaa gctcgttgtt actatgaaaa
     5941 tcacttttca aaagagcagt ttatgctcga actggagaca tgtttagaga gggaaagtaa
     6001 gaaagaataa tttaatgaga gtactgttta tactatctga taatatatat ctaactcctt
     6061 acttcaattt ttataaagaa ttattgaaga aactgtctat tagttacgat gttatatatt
     6121 gggataagaa tattaacgag attattacaa aacaaaacta ctatcgtatt tctttttctg
     6181 gtaaaggaaa attaagtaag atattaggtt atgtaaaatt cagaaaagaa attaaaaaga
     6241 agctaaaaga aaatgattat gatatgatat tgccgttaca tagtattgtg tctttcattt
     6301 tagtagattt tcttctcttt tcatttaaaa atagatatat ttatgatatt cgtgattaca
     6361 gttatgaaaa atttttggtt tatcgtttgg ttcagaaaca attggtgaaa aattctttaa
     6421 tgaatatcgt ttcttcagac ggctataaat tttttttacc aatgggagag tattttacta
     6481 cccataacct acccaatatg atagaattaa acgaggtaaa gcagttaaaa aataatagta
     6541 cgtttccaat tcaactttcc tacattggtt taattcgttt tcaagaacaa aataaaaaaa
     6601 taatcgattt ttttgcaaat gacagtcgat ttcagttgaa ttttataggt actaatgcag
     6661 gagaattaag ggaattttgt caagaaaaaa atatcagcaa tgttaacttg gtggacacat
     6721 tccagcctaa agataccatg tctttttata agaatacaga tgttgttttg aatttatacg
     6781 ggaatcacac tcccttacta gactatgctt tgtctaataa actctacttt gctgcattgt
     6841 tgtataaacc tattttagtt tgtgaagata cttatatgga aaaagtgtca atagagaatg
     6901 gttttggttt cgttttacct atgaaggatg aatcggaaaa agattgtttg gctttgtata
     6961 ttcaaaacct tgacaggaaa cagctaatta agaattgtga taacttcatg gacagaatta
     7021 gtttagaaaa acaaaaaaca gaaatagaat tagagaagcg aatcttaagc ttaaggaaga
     7081 agaatgatta aagtattaca tctatttaca acactagata gtggtggagt agaaagtttt
     7141 ctattcaact attattctca cattgataga aaaaaaattc aatttgattt tattgtgcct
     7201 ggaaaagaac aaggattttt agaggataaa atgaaagaat tgggtgcaaa ggtttatcat
     7261 gtgcctctat taaggaaaaa gcctctacat cagtttctct ctcttgctag aataataaag
     7321 aaaggagatt atgatatagt tcattgccat ggctataaat ctgcaattgg tctgatctta
     7381 tctaaaataa ttggttgtaa aattagaatt attcatagtc atatggctta tgtaacagaa
     7441 aacagttttc aaaaagtatt gcgtaaatta gtaacaattt tggtaaaaat cttagcaact
     7501 cattggtttg catgtgggga agattcggct aagtggttat atggagagaa agcgtataaa
     7561 gacggaaaaa ttgaaattat ttttaatgca attgatttga aaaagtatca atttttgtca
     7621 gatgttagag aaaaatgtcg tagagaatta gatgtgtcaa ataagttcgt attaggaaat
     7681 atagctcgcc tatcagatca aaaaaaccaa agttatttat ttaacgtttt aaaagaactc
     7741 attttaatca aaccaaatgt tattttactc ctagttggta atggtgagga tgagcagaaa
     7801 ttaaaacaga aagctttaga actaaatctg accccatatg tgctattttt agggagaagg
     7861 actgatattt ctgatttatt atctgcgatg gatgtttttt tgcttccgtc taaatatgag
     7921 gggttgcctg tttctctagt agaggctcag gcatcgggat tacaaatttt atcgtcagat
     7981 acagtgacgc aagaagtaga tgtgaccaaa aacattagtt acttacctat caacgaagag
     8041 tctgtgttgc tatggaaaga taaagtactg tctttaacat ctgaggaatg caatcgtttt
     8101 gaaataaata acagtatgac agatggactc tatgatattt gttatcaagc tagtaaatta
     8161 ttgaatcgtt atcaagaaat gtgtgtaata aaggagatat agatgcagac aaagtatatt
     8221 tgtagagtga cactagtgac attaagtttt atatttgctt tctgttactt gttctggact
     8281 ctcgataatt ggaataatgg ttttcttata tctaattatg ttccgtctat atttatatgg
     8341 gtttgctttc taattatttt tcaaattact ggttttattt tacaaaaagt tagtatatat
     8401 gatttttctg tatggtatct gattttatct tattttttta tgtttggatt aattttcaat
     8461 gagtatatgg ggtttcaaac aactctgctg tggagcccta gtaacttcta taataatgaa
     8521 gaattatttc attcatatat ttttataatt tggattttgt tttgttattc tgtaggctat
     8581 ttattttttt atagtgatgg aaaggtacat tatcattcag aagtacaaaa ttatcaggaa
     8641 aatgaagaga aaattttgta caatgcgggt aggattttaa caggagtggg ctttatttct
     8701 agggtaataa ctgattctaa aacagtacta gcagttagag cggcgaatag ctattcagca
     8761 tattcagagg cagctagttc aggaataata gatgatttag gagtacttat gcttcctggt
     8821 gtgttctcct tgttttattc agataagctg tcccgagtta taaagcgtac tattttttgg
     8881 gtaatgttgt tttacctaat tttgataatg atcctaacag gaagtcgtaa aatccaggtg
     8941 ttttcaatct tggcgttggt tttagtatac acacaatctt tagggattac tttttcgaag
     9001 aaaagagtac ttgtattttt aatagtaact gtatttctat taaatgtatt agttgttatt
     9061 agggggcacc gtttcgactt gaatactata ggtatatact tatttgattc cttttcttca
     9121 ttagattttg taaagaatat actaggggaa gtattttcag agtcgggact gaccagccta
     9181 acagtagctt ctgctgtaac tgttgtgcct agtagcattc catatgaata tgggatgaca
     9241 tttctacgca ctatcctatc aatttttcct attggatggt tagttggtga tttttttgat
     9301 aaagcatcag cgacggttgt tattaataaa tttttggggt tacctgtagg ctcttctttt
     9361 gtggaagaat tattttggaa ctttggttac tatggtggtg ttttttggag ttttgttttg
     9421 ggaatattta gtggatggcg attaaatttc agagcttttc aaacaagtaa aatctcaaaa
     9481 gttatttatt tttcggtaat ttctcaacta ttgcttttag tgagaagtag ttcaatagat
     9541 gtttatcgtc ctattatgta tagtttgatt atgatattta tttttaggag gttgaaaaaa
     9601 tagtggtgaa aaaaattatg ttacatggtg caacggatta tggttcaagt aattacggtg
     9661 attatcttta cggtgagatt gtctatgatt tattagaatc taaaggatat gaagtttctt
     9721 tttataatcc atcagatttc tttcaaatgt acttgaaaga atatcgccaa aaacaaagtt
     9781 ttactaaaaa acaagcagat gctattttgt atatcccggg aggatatttt ggggaagggc
     9841 ataatgcacg ttttcgtgat aatttaatcc aatttaaacg atttttacct ttgggaattt
     9901 gggcttcata ttttaaaaag cctataggag tattaggtat tggtgcaggg cctaataacg
     9961 attctttgat gaattatggt ataaagagaa ttattaatca tgctcaattt attacagttc
    10021 gtgatcgaga atcatttgat tctttgaagc atttgtcgcc ttcggctcca gtgcatgaaa
    10081 cctttgattt aattatttca agtaaattaa gagaagaaaa aacagagcag ctgtgtcaat
    10141 tgaaaagaga agctaaagat aagaagataa ttcttgttca ctataatcat tccaaaaaag
    10201 ctcttgaaaa atttgcggaa tcaattagtc tttttctaga aaacaatcct aactattatg
    10261 ttgtagtaac ttcggattcc attctacctt atgaagatgc ttattatcag gaatttagaa
    10321 aattagttag aactgaggat tgttttcaat tcaagtatca ttctcctgca gaaatgacat
    10381 cattgttgaa aatggtagat gtggttttaa cttgtaaatt acatgttggg gtagtggcaa
    10441 catgttttaa taaatctgtg attgccattg catgccatcc agaaaaaaca gcacgttatt
    10501 atggagcaat tggagagtta caacgttgtg aatctttatt tgattcaagt gttaatagta
    10561 ttgtaaagaa acttgaaact tttcatctaa aaccgataac aataccttca gaattggtgt
    10621 tgaaagcgag aagtagtttg gactatttag atcttttttt ggaaggacta gttcgtgaaa
    10681 gttgatagaa tttcatttat aaaaaataca agttctctct atattctgaa tattgttaaa
    10741 ttactatttc ctttattaac actcccgtat ttgacaaggg tgctttcgct agacgcgtat
    10801 ggaatggtta tttatgttaa agcgttaata gcttatgttc aactggtgat tgattttggt
    10861 ttcatgatat cagctacaaa aaatattgta aatgcttgta ctactccctc aaagattgga
    10921 aggatagttg gagatactct agttgaaaaa atatttttat ctatcatttc gattctaatt
    10981 tacaccatat tgatgtggca aatcccaata atgagagaga atattctttt ttcagttttt
    11041 tatttgttag ctacagtgac caatattttt atctttgact ttttatttcg tggaattgaa
    11101 aagatgcatg cagttgcaat tccttatatt atttctaaaa ctatcattac aattttgaca
    11161 tttattgtag taaaagatga ttcttctatt ttatggattc ctatattgga aggaattggg
    11221 aatttagttg ctgcagtagt ttcttataga ttccttcatt attatggaat taaattatca
    11281 ttttcttatc tgtctgtttg ggttaaagat ttaaaggaat cctctattta ttttttatcc
    11341 aattttgcaa ctactatttt tggcgtcttt acgacagtca tttcgggttt ttatttacaa
    11401 agtcaagaga tagccttttg ggggatagca atgcaactgc tttcagcagc aaaatcattg
    11461 tataatccta tagcgaatag tttatatccg catatgatac gtactaaaga tatacaatcg
    11521 gttaagagta ttaatcggat tatgtttatt cctattatct ttggagtttt gatagtttta
    11581 ttcttttcaa atcaaattct ttctataatt ggtggtgaaa aatataccgt ttcagcagat
    11641 tttcttaagt acttattacc cgcttttgtt gctagttttt attctatgat ttacggatgg
    11701 cctgtcttag gagctattga taaagtgaaa gaaactacaa tgacaactat attagcttcg
    11761 attgtccaaa ctttgggatt aggaatattt atcttgtctg ataattttag tttagtaaca
    11821 ttagctattt gttcaagtat gtctgaggtg gtgttatgga ttagccgtta tctaatttat
    11881 tttaagaacc gttcattatt tgttaggagt aagtaaatat atgaaaaaag tagttgtagt
    11941 atttggaact cgtccagaag ccatcaagat gtgtcctttg gtcaaagagt taagaacacg
    12001 taagaatatt gaaacattag tttgcgttac gggccaacac cgtcaaatgt tggatcaggt
    12061 cttggacaca tttgggatta ttcccgattt tgatttgtct attatgaaag ataaacaaac
    12121 tttatttgat gtgaccattg gcattttgga aggaatgaag gctattttag agtcagaaaa
    12181 acctgatttg gtgttagttc atggggatac aagtacgacc ttcgctagtt cactggcggc
    12241 cttttattta cagattccaa ttggtcatgt tgaagctggt ttgcgcactt acgatatcta
    12301 ttcaccatat cctgaagagt ttaatcgtca agcagttggg gttttggctc agtatcattt
    12361 tacaccaaca caactttcaa aagataatct tttaagagaa ggaaaaactc cagagagcat
    12421 atttgtgaca ggtaatacgg cgattgatgc tttacaaact acaattcaag aggactatac
    12481 gcatcctgag ttagaatgga taggagaaag caggtttatt ctaattacag ctcatcgtcg
    12541 tgaaaatctt ggtgaaccga tgagacatat gtttagagca attagaagga ttattgaaga
    12601 atattcagat gtcaaggcta tttatcctat ccatatgaat cctcgtgttc gccaaattgc
    12661 tgaggaagaa ctaagtggct gtgaacgtat caagatgatc gaacctttag aagtattaga
    12721 ttttcacaat tttttatcaa gaagttattt aattttaaca gatagtggtg gtattcagga
    12781 agaggctcca agtttaggaa aacctgtttt ggttatgaga gatacaactg aacgtccaga
    12841 gggaattgaa gcagggactt tgaaacttgt aggtgctgat gaaaataata tttataggca
    12901 ttttaaagaa ttattagaaa atgattcagt ttatcaagca atgagtcaag cttcaaatcc
    12961 atatggagat ggaacggctt gtaagaaaat tgcagacata ctagaaggag aagtgtaata
    13021 tatgtcacaa tttacaggaa aaactctcct aattacagga gggacaggtt catttgggaa
    13081 tgcggttctc aagcgttttt tagaaacaga tgtctcagag attcgtatat tttcgcgaga
    13141 tgaaaagaaa caagatgata tgcgtcatga gtttcaggta aaagtaccag aagtggctgg
    13201 gaaaatccgt ttttaccttg gagatgtgcg tgacttagct tcggtaaaaa atgctatgca
    13261 tggtgtggat tatgtttttc acgctgctgc tcttaaacaa gtaccttctt gtgaattttt
    13321 ccctgtagaa gcggtaaaaa ctaacatttt gggaacggag aatgtcctca ctgcggctat
    13381 cgaagctggt gtaaagcaag ttatctgtct ttctacagat aaggcggctt atcctgtcaa
    13441 tgctatggga acatccaagg ctatgatgga aaagattgct gttgctaagt ccaggacggt
    13501 aaatcctgag catacaaaga tatgtgtgac tcgctatggg aatgttctgt gtagccgtgg
    13561 atctgtcgtt cctctttgga ttgagcaaat taagcaagga aatgctctta caattactga
    13621 acctagcatg acacgttttg tgatgacatt ggaagaagca gtagacttgg ttttatttgc
    13681 atttgaagaa gggaaatctg gagatatact agttcagaaa gcaccagcat gtacgattga
    13741 agtgttggct aaggctgtat cagaaatttt tgcctcagaa caagatatta agattatagg
    13801 tattagacac ggagaaaaaa ggtatgaaac actattaact aacgaagagt gcgcaaatgc
    13861 aattgatttg ggtgattttt atagagttcc tagtgataat cgtaatctta actatgataa
    13921 gtatttcaaa gatggtagta cgaatagaaa tttgttgaca gaattcaata gtaataatac
    13981 cgatttgatg gatgtagagc aagttaaaag aaagttgcta gaattagatg aaatacagac
    14041 agcaattcgt gatatggtag cagacgaaga gatgtaaaat cttttaagat acattaaaat
    14101 tatcaaggat atataatgta tcatgtattg tcgagagagt taactattaa aacagcatat
    14161 aaaataagga gggggcatga ttaaaaatat tttaatcaca ggagcgaaag gatttgtagg
    14221 aaaaaatctt atctgtactc tggaagcttt gaaagatgga cgagatagga ctcgtcctaa
    14281 tttagagatt ggagagattt ttcagtatga tcgtgataca gatccgattt tattagatga
    14341 atattgtaag aaggccgatt tcgtattcca tttagctggt gtcaatcgtc cacagaatcc
    14401 tgatgaattc atggagggaa attacggttt ttcaagtaga ttattggaga ttttagaaaa
    14461 gtatgaaaac acttgtcctg ttctactctc aagttctact caagctagtt tagaaggccg
    14521 attttcaaac tctatatatg gacaatctaa gctagcaggg gaagaactct tctttgaata
    14581 tggaaagaaa acgggagcac ctgtcttagt ttaccgtttc ccgaatcttt atgggaagtg
    14641 gtgccgtcct aactacaatt ctgctgtagc aactttctgt tataatctag ctcacgattt
    14701 acctattcaa gtaaatgatc caagtgtaga attggagttg ctgtatattg atgatttgat
    14761 acaagagtgt ctaactgcat tggaaggaaa tcctcatcgt tgtaatctag atggattaca
    14821 aatcttacct agcccatcag gaaactactg ctacgtacca acgactcatc gtgcaacctt
    14881 aggagagatt gtctctctat tagaaacatt taaaaaacag cctgatagtt tagttatgcc
    14941 tgaaattcct cagggatcgt ttaaaaagaa attgtattct acctacctat cttatttacc
    15001 agtagataag tttaagtttc ctctaaaaat gaatatagat gaacgaggta gttttacgga
    15061 attattaaaa acagaaaata cgggtcaatt ttctgttaat atttctaaac ctggcattac
    15121 caaagggcaa cattggcatc attctaagtg ggaatttttc atggttgttt ctggtcgtgc
    15181 tttaatacaa gagcgtagga tagggctgga tgaaaacggt caagagtatc ctattctgaa
    15241 ctttgaagtg tcgggcgata agattgaagc aatacatatg ataccgggct atgcacataa
    15301 tattattaat ctttctgata cagaaaatct aattactgtc atgtgggcta atgagtcatt
    15361 tgatcctaga catccagata ctttttttga acaagtggag aaataaatga aaattaagac
    15421 agattatagt gatattcact ttaaagataa tggcaaactt aagttattga ttattgtggg
    15481 aacacgtcca gagattatac gtctaagtag tgttatcact aaatgtcgaa agtattttga
    15541 tgttattttg gcacatactg gacaaaacta tgattacaat ttaaatggta ttttctttga
    15601 taatttaggg ttagacactc cggacgtata catggatgct gttggagatg atcttggtgc
    15661 tactgtaggg aatattatta atacttcata caaattgatg aatcaaatta aaccagatgc
    15721 tttattgatt ttaggggata caaattcttg tttatcagct attgctgcca agcgtttaca
    15781 tattccaatt tttcatatgg aggctggcaa tcgctgtaaa gatgagtgcc taccggaaga
    15841 gactaatcgt cggattgttg atgttatttc agatgttaac ttagcatact ctgaacatgc
    15901 acgtaagtat ttacatgagt gtggtttacc taaagagcgc acatatgtaa ctggttctcc
    15961 tatggcagaa gtgttacata aaaatttatc tgctattgag tcttcagata tccatgaacg
    16021 tttgggattg aaaaaaggag gttatatctt actttcagct caccgtgagg aaaatattga
    16081 tacagataaa aattttattt ctctctttac agcaattaat caattagctg aaaagtataa
    16141 tatgccaatc ttatattctt gccatcctag atcaaagaaa aggctacaag agagcggttt
    16201 taaactagat aaacgtgtga ttcagcatga gccactagga ttccatgact ataattgttt
    16261 acagatgaat gcatttgttg tagtatctga ttcgggaact ttaccggaag aaagtagttt
    16321 ctttactagt caaggttatc cttttcccgc tgtatgtatt cgtacaagta cagaacgtcc
    16381 tgagtcctta gataaggcag gatttatttt ggcaggcatt gatgaaaatt ctcttcttca
    16441 agcagttgaa actgctgtta gcttggcaca agatgaggat tttggattac cggttccgga
    16501 ttatgttgag gaaaatgtct ctactaaagt tgttaagata atacagagct atacagggat
    16561 agtggataaa atagtttgga ggaaaagctg aattgtatat ttttaataaa tcaaaacgag
    16621 atgattaatt cttagtagaa caagagtaaa attgaacgta tttgatggaa atgtcttact
    16681 tgatagtcga gggatagcca ttctatcgga tttttggctt gtttttgcgc ttcatgtttg
    16741 atagaataaa gggctctttg tcaactgtag tgggttgaag aaaagctaag cttgagaaag
    16801 gacaaatttc gtcctttctt ttttgatgtt cagagcgata aaaatccgtt ttttgaagtt
    16861 ttcaaagttt cgaaagtaac cgcccaataa cgaagtctat tgaaaaatct ccagactaga
    16921 gaactcatat atagttccta atctggagat ttcttatttg cacttttctt gtacaacttt
    16981 agtccacggt aaataggcct ctaaaacctc tttgtttacg agagtttcct cgtttggaag
    17041 acattctaga agataggata gatatttctc gctatttata atggattgta ataagatatg
    17101 aacaaatcga ttaggaaagg aaaattgatt tatagaaata ttttagcagt cgatgcgtac
    17161 tgttatagat tcaacgcact ataattgatg acgtttagct gtttccaaca aactcataat
    17221 aatagctctt gctttagctc cttcaaaact ttgagaaaat aaccaccctc gcttttttaa
    17281 aaataaccac cctcgttttt ttaaactgag cttaggctaa aagagtccac tggactcttt
    17341 tactccgtcc cataaccaat gatttaatgg cgcgttcagc tagattattg gaaaggacca
    17401 gatgtccgtc ttttaaaatg gtcttaaagg tttcttcata cttgaggctg tattcaattg
    17461 cccttcctag ttttgaaccc gataaaactg actgacgacg gcaccaagca aagaagtctt
    17521 ccagtagggg ttggagatgt tcttgacgtt tctgtagccg ttcatcagct ggcaaagcct
    17581 cccagtctct ttccaaggaa aataact
//
LOCUS       DBDESG010000052        17344 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.052, whole genome shotgun sequence.
ACCESSION   DBDESG010000052 DBDESG010000000
VERSION     DBDESG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.052"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..357
                     /locus_tag="ACKB38_001676"
                     /pseudo
     CDS             1..357
                     /locus_tag="ACKB38_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(474..821)
                     /locus_tag="ACKB38_001677"
     CDS             complement(474..821)
                     /locus_tag="ACKB38_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGL7980408.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYANDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(932..2707)
                     /gene="spxB"
                     /locus_tag="ACKB38_001678"
     CDS             complement(932..2707)
                     /gene="spxB"
                     /locus_tag="ACKB38_001678"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGL7980409.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2914..5157)
                     /locus_tag="ACKB38_001679"
     CDS             complement(2914..5157)
                     /locus_tag="ACKB38_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGL7980410.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5167..5538)
                     /locus_tag="ACKB38_001680"
     CDS             complement(5167..5538)
                     /locus_tag="ACKB38_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGL7980411.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5549..5944)
                     /locus_tag="ACKB38_001681"
     CDS             complement(5549..5944)
                     /locus_tag="ACKB38_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGL7980412.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVDKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6128..6226
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6310..7101
                     /gene="thiD"
                     /locus_tag="ACKB38_001682"
     CDS             6310..7101
                     /gene="thiD"
                     /locus_tag="ACKB38_001682"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGL7980413.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7311..7943)
                     /gene="thiE"
                     /locus_tag="ACKB38_001683"
     CDS             complement(7311..7943)
                     /gene="thiE"
                     /locus_tag="ACKB38_001683"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGL7980414.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(7936..8739)
                     /locus_tag="ACKB38_001684"
     CDS             complement(7936..8739)
                     /locus_tag="ACKB38_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGL7980415.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8739..9263)
                     /gene="thiW"
                     /locus_tag="ACKB38_001685"
     CDS             complement(8739..9263)
                     /gene="thiW"
                     /locus_tag="ACKB38_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGL7980416.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9268..9960)
                     /gene="tenA"
                     /locus_tag="ACKB38_001686"
     CDS             complement(9268..9960)
                     /gene="tenA"
                     /locus_tag="ACKB38_001686"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGL7980417.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9971..10621)
                     /locus_tag="ACKB38_001687"
     CDS             complement(9971..10621)
                     /locus_tag="ACKB38_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGL7980418.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALTLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSVSLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKI
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10623..12008)
                     /locus_tag="ACKB38_001688"
     CDS             complement(10623..12008)
                     /locus_tag="ACKB38_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7980419.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12009..12569)
                     /locus_tag="ACKB38_001689"
     CDS             complement(12009..12569)
                     /locus_tag="ACKB38_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGL7980420.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12831..12919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13128..13757)
                     /gene="thiE"
                     /locus_tag="ACKB38_001690"
     CDS             complement(13128..13757)
                     /gene="thiE"
                     /locus_tag="ACKB38_001690"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGL7980421.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13759..14541)
                     /gene="thiM"
                     /locus_tag="ACKB38_001691"
     CDS             complement(13759..14541)
                     /gene="thiM"
                     /locus_tag="ACKB38_001691"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGL7980422.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLKTAVLVY
                     NIAGEIAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14778..15446)
                     /locus_tag="ACKB38_001692"
     CDS             complement(14778..15446)
                     /locus_tag="ACKB38_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGL7980423.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15661..15749)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16003..17139)
                     /gene="lctO"
                     /locus_tag="ACKB38_001693"
     CDS             complement(16003..17139)
                     /gene="lctO"
                     /locus_tag="ACKB38_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGL7980424.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         5222 a         3826 c         3176 g         5120 t
ORIGIN      
        1 aatcattttt cttaatagct ggtagatgta atactgagtg gatgaacgcc tttttagaag
       61 agctttcaca agcttatcct tttactcgtt atggacaatg ctatatggca taaatcaagt
      121 accttaaaga ttccgactaa tattggtttt gcatttattc ctccatacac accagagatg
      181 aaccccattg aacaagtgtg gaaagagatt cgtaaacgtg gatttaagaa taaagccttt
      241 cgaattttgg aagatgtcat gaatcaactc caagatgtca tacaaggatt ggagaaggag
      301 gtgataaagt ccatcgttaa tcggagatgg actagaatgc tttttgaaag cagatgagta
      361 ttatatgcaa tttctttata taaaaagacc ggattgctcc gatctttcaa tagttcatat
      421 tctcaatttc tattttaaaa atagctaagg ttaacgtcaa atgactacgc gacctatttc
      481 atacgataaa aatcaagcac tagaccagca ggtccttgaa ctaataagga ctctgttccc
      541 caatcggtta cagttggtcc gtgtaaaacc tttataccaa gctcgttcaa ccgtttgtag
      601 ttctggtcta catcctcaac ctcgatatga ataatgattc ctgactgaaa gttttccaaa
      661 ggaaccaaat gattttgtga caacataagg cagtgactac caatcgtaaa ctgagcaaaa
      721 ccatcattag cataatctgc ctttttatcc aagatatgct ccaagtcagc acagacttgg
      781 ggaacatttg aaacgataat atctaattga tttaaattca tttactctcc tccataaaaa
      841 gaccggattg ctccgatctt ttaaagttct gctctatgaa aatcaaagaa taaagtctac
      901 aagtttcata tttgattttc ggcgagagga attatttaat tgcgcgtgat tgcaatcctt
      961 cttcttccaa gaagagacgg aatggtacga gttcttctgc ttcgtatttt tccttgaagg
     1021 ctttgatagc ttcttctgag tgaagttttg gatccaattc aagtacttct actggaagtg
     1081 gacggtgttg agtgatgcga gcatcgatga caacagtttt accttctttg ttcaatttaa
     1141 cagcttctgc aacaactgca tcgatgtctt cgatacggtc aactgtgaat ccaacagctc
     1201 cttgagcttc cgcaatttta gcgtagtcag cgtttgtgaa gtctacacca aacaagtgtt
     1261 tgtttgtatc ttcgtatttg ttcttgatga agccgtactc agcatttgag aagacaaggt
     1321 tgataactgg aaggtcgtat tgaacgtttg tgataacgtc tgggtagcac atgttgaatg
     1381 ctccgtcacc catgatgttc catacttggc gatctggatt gtctttctta gcagcgatac
     1441 caccaggaag ggcaataccc attgtcgcaa agagtggaga tgtacgccac atgttcttag
     1501 gtgtcatgtg aaggtgacga gtagatgttt gagtagtgtt acctacgtcg attgagtaga
     1561 tagcgtcttg atcagcatgt ttgttgattg cattgtaaac ttgatacaat tgcaattcac
     1621 cctcagtttt accttcgagt ttgttcatgt aatcacgcca gttttggttg ttcttaacgt
     1681 ttgcacgcca ccatggagtt gattcaactg ggtttacttt gtcaaggata gctttagctg
     1741 cttgaccagc atcaccaagg attgaagcgt caagggcatg acgtttacca agtttgtaag
     1801 ggtcgatatc gacttggatg aatttttcag tgttcttgaa tgcttcgtaa acttcagcaa
     1861 atgggaagtt tgaaccaagg aaaagaactg tgtctgcttc aaagaccact tcgttggctg
     1921 gtttccaacc aacacggtaa gcagaacctg tcaaaccttc atagttccat tcgaaagctt
     1981 caaagttttt accagttgtg atgattggtg ctttgatttt acgtgacaat tcagtaatca
     2041 cttcaccagc tttaacacca ccaaatccag catagataac tgggcgttca gcattgttca
     2101 agatttcaac agctttgtcg atttcaactt cgttcaaagc aggagcgatg aatgagcgtt
     2161 cgtatgaacc tgaaccgtag tatgagtttt catcgatttc ttggaaaccg aagtttactg
     2221 gaatttcaac aacagctgga ccttttttag aaactgcagc acggcaggct tcgtcaatta
     2281 cttttggcaa ttgctcagcg taagctacac gtttgttgta aacagcgata ccgttgtaca
     2341 ttgggttttg gttaagctct tggaaagcat ccatgttcaa ttcgttaact ggacgtgatc
     2401 caaggatcgc taggaatgga gtgttatcca tagctgcatc gtaaacaccg ttaatcaagt
     2461 gagtcgcacc tggaccacct gaaccaactg caaccccgat tgagccgccg aatttagctt
     2521 gcataaccgc tgcaagagca cctgtctctt cgtggcgaac ttgtaagaaa cggatatctt
     2581 tgtcttcagc caaagcgtcc atcaatgagc tgagtgttcc tgatgggata ccgtagattg
     2641 tatctacgcc ccatgttttc aatacgttaa gcattgctgc agatgcagta attttccctt
     2701 gagtcataat gataactctc cttcaatttt tttaaacttg gagaatacga ttacatagaa
     2761 ttggaaacgt tctccaaatt tttactattc cactgtatca tatttatgct gacttttcta
     2821 aaaatctgct caaaactctc tattctctat tctaatacag ttttgaaagt tctgtcattt
     2881 ctgttttata acaaagaaat ctagtcatta cttttagtct attttactaa aatttaacag
     2941 aagggaactg gtcagaacag atacagaact aaaggccatg gctagacctg ccaattctgg
     3001 gttgagagcc agtccaacac ctgaaaagac tcctgctgca atcggaattc cgacaacatt
     3061 gtagataaaa gcccagaaaa gattgagtag aattcgatga aaggttttct tactcatatc
     3121 aaaggcacga accactccta aaagattatt ggttgtcaac accaaatctg ctgactcgat
     3181 ggcgatatct gttccagctc ccatagcaat ccccacatct gctacactaa gggcaggagc
     3241 gtcattgata ccgtccccaa caaaggctac tttccctgac tgttgcagtt tatggatttc
     3301 atgggctttt tcttctggca agacgcctgc aatgacctct tcaattccga tttgatctgc
     3361 aatagcacgc gccacaccag cattgtctcc tgtcagcatg actgttcgga gaccacgttt
     3421 ttttagctga ctgatggcta gcttagcatt ttccttagga atatcttgca aagcaagcaa
     3481 gcctttgatt tcattgtcaa cagctaagaa cacaactgtc ttagcttctt tttctagttc
     3541 ttctagttta tcttgataag tattagaaat atccatgcca tccagcattt tagcatttcc
     3601 aagtaaaact tgttttccat tgattcgccc tgaaacacct ttcccgtgca aggactgaaa
     3661 attttcaaca gtttgaaact caagtccagc ttcactcgct cgcttaacga tagcctcagc
     3721 cagtgggtgt tgagaagcat cttccaagga ggctgccaac ccaaacactt ctacttcgtc
     3781 gccgatgaca tctgttacca caggtttccc ttccgtcaaa gtcccggtct tatcaaagac
     3841 aagggtttga actttctgga tttcctgtaa gacagttcca tttttgagga gaacccccat
     3901 cttggcacta cgtcctgtcc ccaccataag ggctgtcggt gttgcaagtc ccaaggcaca
     3961 aggacaggcg ataatcaaaa ccgccactcc gtagagaaga gaggacacaa agctagctcc
     4021 aagcacaacc acactatccc tgagcaagac gaaccaaacc caaaaggtca tgattcctaa
     4081 aatgacaact actgggacaa aaatccctga aatcttatcc gtcaagtcct gaatcggcgc
     4141 acgacttgtc tgagctttct tcacaaaatc cacaatctga gccaaaacag tctctgagcc
     4201 aactttttct gctctaaaga caagcgttcc actatgattg atggttgagc caatgacagt
     4261 atctccaact gtcttgtcca caggcagact ctcacctgtc accatggatt cgtcaatact
     4321 agagacacct tctactacga caccatcaac agcaatcttt tcaccgggac gcactcgaat
     4381 caggtcgcct accttgactt gttccaaagg aacttggaca taactatcat cactcaagac
     4441 ttctgcggtt ttagcttgca agtccagtaa tttctccaca gcttgggacg tattttttct
     4501 cattttttcc tcaaaaactg ctcccaaaag aacgaaaaag aggataaatc cagcactttc
     4561 gaagtaaaca gggagaccag caaagagagc aactaggcta tagaaataag ccactagagt
     4621 tcccagcgca accaaggtat ccatgttggc attgtgcttt ttaaaactgg cccaagcact
     4681 ctggatatat ggcttacctg caactaacat aataggcgtt gttgctagaa aggttcccca
     4741 atgcatgact tgatgactaa tgctacctgt caacatccca atcatgagaa tcacaagagg
     4801 cacagtaaag atactagtaa tccaaaaacg ttgcaggaga gatagagatt ttcgagtctt
     4861 ctcaacgact gtatagcttc ccttttgcat cttcatgcca caagaaaatt catgtcgccc
     4921 taattcttga ggcgtaaaac gaatgacttt ctcctcatct acgccgattg gttccaagat
     4981 accttcttct tcaaacagaa tttccttata acagtttgaa ggagtagcac gatgaaaggt
     5041 aatctcagct ggaattccct tttgaagctg gatatgggct ggatgatagc ctttttcagc
     5101 tcggatacgg attttttgaa tgccattttc taagcttgct ttcacaattt ctgtcatagt
     5161 ctccacctac tctacaatca tcttgccgtg catcatgttc ataccacaag caaagccaaa
     5221 ctctccagcc tgttcaggcg tgatttccac tacatactct tcccccattg gcaggttcgc
     5281 atgtacacca aaatctggaa aaacaatttg atccagacat ggtgaaggat ccttgcggtc
     5341 aaagacaatg cgtgctggca ctgatttctt gaggacaatc aactcaggag tatagcctcc
     5401 catgacttcc actcgaatct cttggtatcc gtttttttgc tgggcttttt gtccagattt
     5461 ttcaggcttt ttgaaaaacc aaaacaagat aaacgcgata agggcaatac aaataatggt
     5521 tacaatacta tttaacatga cgtctccttt acatacaatt acatcttact tctgttacag
     5581 cacttgattt cttctctgaa atcacagctt ccaagtcttc caagtcagtc tgagtaaatt
     5641 cacattctac aatcaagtca gccaacaaat tcctaatcct acgggaacaa accttgtctt
     5701 tgatatcttg gacaagtaaa tcccgacttt ggtctagagt taaaagggct gaataaacaa
     5761 aggacttgcc ttcttttttc cgagtcaaac actctttatc aaccagacga gccaaaagtg
     5821 tctgaaccgt ggacttggac cagtcaaacc gctctgccaa aaccctaatc aaatctgtac
     5881 tggtctgctc cccctgcatc caaataatct tcatgacctg ccattctgca tctgaaatct
     5941 gcattaccat acctccaaaa tctacatttg tcaattacac tcatcagtat actcttaaaa
     6001 tctacatttg tcaattatag aaataatatt ttcttcgaaa aatagaattt taatcatttg
     6061 aaaaacgatt tgcagtcaaa tattactata taaacaataa aaatatgcta tactaaagaa
     6121 aaaagaaaac aaccactagg ggtgcgtaaa gctgagatta acgactgtta gatccctctg
     6181 actcaatcta ggtaatgcta gctgatggaa gtggaaatga taatggggac tagcagtctt
     6241 ctattgcctt tctaaaacag actagcttgt tcttaagaat acaaacttca gttggttggg
     6301 aggttttaga tgacttattt acccgttgct ttgaccattg cagggactga ccctagtggt
     6361 ggtgctggca ttatggcaga tttaaagtca ttccaagcga gagatgtcta tggaatggct
     6421 gttgtaacca gtcttgtcgc tcaaaatacc agaggtgttc agctaatcga gcacgtttct
     6481 cctcaaatgt tgaaagccca attggagagt gtcttttctg atattccacc tcaggctgta
     6541 aaaactggaa tgttggctac tactgaaatc atggaaatca tccaacccta tcttaaaaaa
     6601 ctggattgtc cctatgtcct tgatcctgtt atggttgcta caagtggaga tgccttgatt
     6661 gactcaaatg ctagagacta tctcaaaaca aacttactac ctctagcaac tattattacg
     6721 ccaaatcttc ctgaagcaga agagattgtt ggtttttcaa tccatgaccc cgaagacatg
     6781 cagcgtgctg gtcgcctgat tttaaaagaa tttggtcctc agtctgtggt tatcaaaggc
     6841 ggacatctca aaggtggtgc taaagatttc ctctttacca agaatgaaca atttgtctgg
     6901 gaaagcccac gaattcaaac ctgtcacacc catggtactg gatgtacctt tgctgcagtg
     6961 attactgctg aactagccaa gggcaagagt ctttaccagg cagttgataa ggccaaggcc
     7021 tttatcacaa aagctattca agatgcccct caactcggtc atggttctgg tccagtcaac
     7081 catacaactt ttaaagatta agaaaaaaaa ctctctagtt cccactttaa gggaattaga
     7141 gagtttttat actcttcgaa aatctcttca aactacgtca gcttccatct gcagcctcaa
     7201 aacactgttt tgagctgact tcgtcagtct tatctaaaac ctcaaggcag tactttgagc
     7261 aacctgcgac tagctttcta gtttactctt tgattttcat tgagtattaa ttaggaaaga
     7321 atgttatgca acttttttaa aaaggcttgc gtttttgcct caatatcttc tgcttgcatc
     7381 aaatcacgta caacagctac accagctatg ccagtgccca taagctgatc aatattctcc
     7441 gaagtcaagc ctccaatagc aactactgga atggcaaccg tttggcaaat tgttttcaag
     7501 gtcgatatca gagtaatggg cgcattttcc ttggtggtgg ttgggaaaat ggctcctgta
     7561 cccaagtaat ctgcacctga tttctccgct tccagagctc ttttaaccgt tttagcggtg
     7621 acaccgagga ttttttcagg acccaagact ttgcgagcta ccgaaactgg taattcatca
     7681 tctccgatat gcagacctgc tgcatcaacc gcaagacaaa catccaaccg atcatcgatt
     7741 atcaagggta cctgataagc atctgttatt tccttgactt gttttgccag ttgataatat
     7801 tgattggttg tgagattttt ttctcgcaat tggactatgg taacccctga acggcaggcc
     7861 gtctcaactt ttgcaagaaa gctttccacg gaatcttgat agcgattggt taccagatat
     7921 agtctaagtg cttctctatt cataaacctc tcctttgatg gtatctagcc aattttcatc
     7981 tcttcttagg agcgaaagct gattgagtac ttggtaacga aattcttcca atcccattcc
     8041 ttgaacaact attttctcag cagcgatatt gagataagag actgctaagc aagaagcttc
     8101 aaaaccagtc tttccttggc tgagaaaaac agctgttaag gctccaacca agtctcctgt
     8161 ccctgttatc cagtctaatt cagtacagcc atttcccagt acagcgacct gatttttcga
     8221 aacgacgagg tccttgggac ctgtgactaa gaaagacata ccaggatagg tctgacacca
     8281 gtctttcaag acttgaagca aatcctccgt ttcttgatct ttagcactcg catcgacccc
     8341 aacgccgtgg tgctttaatc caacaagact tcgaatttct gacatgtttc ctttaaggac
     8401 cgtaggtcta tagtctaaaa ggtctttaac taagctctta cgaatggatg aagtcgttac
     8461 gccaaccgca tctactacca tcgggagaga agattggttt gcatacgaag ctgccatgcg
     8521 gattgctttt tccttctcag ctgacaaatg ccccaaattg atgaagagag cctgactttg
     8581 cttagtaaaa tcaagaactt cacgggaatc atctgccatg acaggtttgc atcccagagc
     8641 caaaatccca tttgccagca tctcacaaga aatctcattg gtaatgcagt gaatgaggga
     8701 actagagcct ataggaaagg gatttgtaaa ttcctgcatc agtctatcct ttcactaaag
     8761 aaatatccct gcactttttt aaagaattcc tgcttgatta aaaatcgaaa ggcaataaag
     8821 gaaatcgctg taccaatcaa ggttgctccg aaaaatcgag gcgtgtagat aaaccagcta
     8881 agcttagcag ctgatcctgt aaagagtacc ataacaggat aggaaacaat ggaaccaata
     8941 atacctgttc ccaaaatctc tcctagagca gaatagtgaa attttcgacc gtacttataa
     9001 aagagacctg ctagaagggc tccaaaagtc gctcctgtga gagctaaagg cggaatccct
     9061 tgagtcgtca tacggataaa ggctgtgact gtagccatag ccaaggcata aacaggtccc
     9121 atcatgattc ctgctagaat attgactaca ctagacatcg gtgccattcc ctcaattcga
     9181 aagataggtg taaggactac atcaagggca atcatcatag ataaaatggt taatttgtga
     9241 acttgtaatt ggtgctttct catgcttcta ttcttctcct ttttctaaag actgtaaatc
     9301 gctcttccat gtctggtgtt ggtaggccat ttcccaaaac ttggcttcca tatgaacact
     9361 gatgtggaag gcatctagca ttttttgctt gtctgtctcg tcactttctc gatagagctg
     9421 attgaccagt gctccctcct ctctgatctg ttgctctaac tcatccgtaa tataagtttc
     9481 aatccattgt tgatagagag gatttggtga tggtttaaga ttaagtgatt tgcctatatc
     9541 atggtataac caaggacaag gaagcaagct tgcaaaagcg atggctaagt tcggttctgc
     9601 aaattgccta taaatatgag aaatgtaatg ataacaggtt ggagcgattg gatgttgctc
     9661 catttcctgg tcgctgattt ccaattcctt gaaaaattgt tggcgaataa ataactcacc
     9721 ctccactaaa ccctgagcat tttgtttcaa gagtcttttc atctcttggt ttgaagtctt
     9781 atcagccaaa agatgataga tttctgagaa agccttcaga tagtaggcat cctgaatcag
     9841 gtaatagcgg aaaatggcag gttctaaatt cccctcttgt aattgtaaaa taaagggatg
     9901 atgaaaggaa gcctgccaag ctttcttgga taattccatc gcaatatctg taaattccat
     9961 aataactcct ttataaaaat agactggttt gaagcaataa aaagaaaagc aggtagatta
    10021 attttgtttt tttaggaata taaaaagtcc gatagctatt cttcaactgt gcatgttcgt
    10081 catatccgtg agcagataga gctctcaggt aaagatggcg ccacctaaag actgtcatca
    10141 gaatcttact gtaaatcaag ggcgaccaaa aatgtagttc ttgaccacgt aataggcaag
    10201 cttctttgag ggacttgatt tcttgctgaa tgagaggaaa agaattgaat accacaatca
    10261 aggcatagga ccaagagcgt gatagcccct tttgagccaa gtacaagaga agctctttta
    10321 gtgaaacaga ggaaacaaag acaaggccga tacaaactgt cacaaaggcc ctcgttccaa
    10381 gcatgactgc ctgtgaagca tctccgtgta actgaactgc ccagtagttg gcaaaagatg
    10441 gtaaaatggc aagtatgatc atccaagcta acattttaaa tcgacggtaa tagagcataa
    10501 agagaataca aaatgcgact accgaaagag tcagagcaat cgaaggaatg aaagatgttt
    10561 ccaaggataa aatcagcaag aagagactga taatcggtgt ctgggttgct actttgacca
    10621 tactatctca cctccccttg ggtattgcta ctctgagatg taagtggttt ggtaatggtc
    10681 acttctttca catgccgaag accctgacta gtcatctcaa tccaataatc aaccacagaa
    10741 atcaaagggt ctaaacgatg actaatgagc agaaaacttc ttccttgatt cctctcctcc
    10801 acaatccact tgcaaaaata atggcaggct ctatcatcca aacctgcaaa aggttcatct
    10861 agcaagatca cggaagcctt actggtcaag atggtcagga gctgaagaat tttttgctga
    10921 ccaccactta attgataggg actcttatcg actgcctgct ccaaatcaaa atatcgtaaa
    10981 gcttgaaaaa tccgctgatt tctttcagaa tcaggtccat ctaattgaag ctcctctcgc
    11041 agactgactc ggataaactg cttctcagct tcctgaacaa caccagtcag atcacgatac
    11101 aaactctttt tctttttcag gaccgaaccc ttccaagtaa tgctcccctt atacttttga
    11161 aattgaagaa tggaccgaaa gagggttgat ttcccgacac cattgtcacc caggatacag
    11221 gaaatccctt gatagaatgt gaaatcagca attgaaaaga gggggcgatt accaagctca
    11281 ccagtcacac ggttcatatg gaatagttcc gggctagaag caacttcctt tgaagcaacc
    11341 tgtgtcatct cataggaagg gatttgaaac acttccctta gttttccgtc tcttagctcc
    11401 accatatggt cgatataggc tttatagtca gataaatcat ggtcgcacaa aataactgtc
    11461 ttcccatcat agaccaactc ttttagaatc tccaatatct cgattctgct cttgcggtca
    11521 atggaagcga agggctcatc caagagatag accctaggat tcatggcaaa gaggacagcc
    11581 agcgctgctt tttgcttttc cccacctgat aagtgatgga tgagacggtg caagatgtcc
    11641 ttgcaacgac attgctggac aacctctgct attttagaat caatttcctg aaggtgatag
    11701 ccgatatttt ccatggtaaa aaccaactcc tcaaacaagc tctccatggt aaattgatga
    11761 ttaggatttt gcaagagaat accaaccgtc tggacacgtt cgacgataga aagctgactg
    11821 acctcgctcc catctatcag gacttgaccg ctatagggaa gagaactaac ttgggcaatc
    11881 atttgaaaga ggctggattt tccagaccca ctactcccaa ctaacaaggt aaaggcttgc
    11941 gcatgaaaag taaaatcaaa cggctcagag aagattgggg actgaatcgc tcgtagttcc
    12001 agacccatct atgcttttcc tccagttgca aactgatgat agagtttgac aatggcacga
    12061 accaagatgg tacagaagaa ataaacagaa ataaaacgta ccacaagcaa ggaaaggaca
    12121 aacggaaggg aaaaggcgta gtaacctaac ttaatgtatt catagacaaa gctaacaagc
    12181 gtaatcccaa tactattagc agttagagag agccaacttt catagcgatt cttagttacg
    12241 ataaaaccaa attcacttcc caaaccttga acaaagccag acaaaagagc tcctagacca
    12301 aattggctac cataaaggac ttcagcaagc gcagctagca cttctccaat cgttgcactt
    12361 ccgactctcg gaacaaagat ggcagcaatg ggcgcagcca tacaccagag accgaagagg
    12421 atttcattgg caaaggcctg caaaccaaga ggtgttaaga gtagactgag aatattatac
    12481 acatatcctg aaccaacgaa aaccccacca aaaaagatag acaagaaagc aagcaagata
    12541 acatctttta actgccattt tttcaacata aaaaactcct ttttttaaag aaaagtgagg
    12601 cactcaagaa gaccgaccta aatactttgt atagcagact gaatttagaa cagtacacaa
    12661 gaacactaaa atatttctag aaattaattt gaattttcta attgatttgt tcgcatctta
    12721 tttcaatcta ctatatcatc ttcatccagt ttcgtaaaag aaaaaactct aattacagat
    12781 acaaattaga gttcagctta caagattaga cagttctttt cgacatacga aaaaaacatt
    12841 tcacatttcc cttcgccagt cttaactgta tcaggttcaa tgggtatcat ctcagcctaa
    12901 agcaccccaa atgtctttat tatttaatta tgtgattatt ataacacaca ttttatacta
    12961 gttcaagaaa ttgaactgga aatacagcct tgcactcaca aagacagcag atctttcttt
    13021 tgcaaaaaac aaatgacctg tttgatgaat tagccattca agctgaatct ggacatagct
    13081 ttttaaaaaa ggaaaatcct acttacttag aatccaagga tagatatcta ttgttcactc
    13141 atttcccgaa cagttttttc tatatttttt gcatacgata ttgccgaaat gattgaaacg
    13201 ccatccatat tggtctttat aatgtcttta atatgtttcg tctgtatccc accaattgca
    13261 actaaaggca tttgtggcaa tagttttctc atcaattcaa gaccttcata acctatagta
    13321 ccaccagcat catcctttga ctgggtacca aatacaggcc caacacctac ataatctaca
    13381 tattcaactt ttgattgttg aaattcttct tcgtttctta tagaaagacc aattatttta
    13441 tctggcatca attttctaat ttcatcaaca ccaatatcat cttgacctac atgtacgcca
    13501 tcggcgtcaa tttccattgc taaatctata tcgtcattaa cgataaatgg aacattgtat
    13561 tttttacaaa gttctttaat ttggatagct agctcaagtt tttctaagcc ttctaaagca
    13621 ccctcacctt tttctcgaaa ttgaaataag gttataccac cttttaaggc ttcctcaacg
    13681 actgtatata gattttttcc ttggcaagta gtcgttccac aaataaaata tagttttagt
    13741 aattctttat gaaacatctt acttcactct tttgaattcc tttacatctt catctgtaat
    13801 ctcgtataag gcatttataa attcaacttt aaatgtccca ggaagatgtc catttggacg
    13861 tttttctgct atttctccag cgatattgta aaccaacact gctgttttta atgatttcaa
    13921 ttcttgacct ttttctagtc cgataaagct tgctactaca gctcctaata agcatcctgt
    13981 cccaatgact ttcggcatca tagcactacc attatgaatc attaccactt ctccattaac
    14041 agcaatggca tccacttcac ctgttactac tattggaata ttgaacttct catttgctgc
    14101 tagagcaatt tcgtcaatat tatctacgcc cgcactatct actcctttag atgccacatc
    14161 tattcctact aaagaggcaa tctcgccagc atttcctcta atcgctgcta gtttataatt
    14221 gttgattaga tcatctgcta ctttttttct atattctcct gctccacagg ctacaggatc
    14281 taaaactgct gggacattat atttctctgc aattttcaga gcagcttggt ataatttcca
    14341 attttcatct gtcaatgttc ctatgtttat taataaacca ccagcatact ttaacaaatc
    14401 ctctaaatct gctggaaact cactcatggc tggtgaggcg cccagtgcta ctaatccatt
    14461 tgctgtgaaa ttttttacta catcattggt tatacaaatg accaatggtg ctttttcttt
    14521 taataatttt aaacttgtca tattgaaatc cttccttttc actttatacg atctactaat
    14581 ttcgatttat ctttagttga gaattttttt catttacatt gaatgattta tactcaatga
    14641 aaatcaaaga gcaaactagg aggctaaccg caggttgctc aaaacactgt tttgaggttg
    14701 tggatagaac tgacgtggtt tgaagagatt ttcgaagagt cttacctcat caaatttgta
    14761 aatatcatga gccttctcta gacatcgtaa ccaatatcaa aaaaagctaa ttctaaagcg
    14821 actgcttgat tccagcgttg ctgaagttct gtcaaatctt ctcgattttt accgacacga
    14881 ttgagttcgt caaccagaaa ttgaacccac tctgcaaaga aaggacctct gtggagattg
    14941 atccattccg aatgaatata gacttcaggt aaagccaaat ctttagaacc ccagtctaaa
    15001 tagagacctt ctgcaatgac cagcatgacc aaaagatggg catagtctga tgaagccacc
    15061 gccgaataca ttagatcctg aaaggctttt gttacagggt gcaaagtcac ttctagatag
    15121 tcattctctg ctacttttaa ctctttaaaa gccttttgga aataaccatc ttcatctgct
    15181 tcaagaaagc ctagttgctt ggcaaaacga agcttggatt caagtttatc tgcgtgagct
    15241 acgcaggcac ccagcatgga taagaaggca tcaaagaagt gataatcttg aatcagatag
    15301 tcctttaaga ccttattctc aattgtcccc gcaaaaagtt ccttaacaaa acgatgattg
    15361 attgcagcct gccaatcctt ctgactgctt tttaataatt ctccaacagt caaacctggc
    15421 tgaaatgcat agtcttgtgt ttccatattt acttctcctc tctttacttg ttagtaatta
    15481 ataaaacacc aagaaatatc aagcaaaatc gtaattccac ttgatccttt taaagcacat
    15541 cgagagcatt tgcagagagc taactaaaca agcctatcca gtttatataa acaaaaaact
    15601 ccaattacaa tcaagaatta gagttgactt acaagattag accgttcatt tcaccatacg
    15661 aaaaaactgt tcacatttcc cttcgccagt cttaactgta tcaggttcaa tgggtattat
    15721 ctcagcctaa agcaccccaa atgtctctat tatttaacta ctgaaccagt atagcaaaaa
    15781 atgaaagccc tagcaagata tttgaccgaa aaatatcttt atatataata tattgaaact
    15841 agaatagtac acctctactt ataaaacatt gttagaaatc gatttgactg tcctgattga
    15901 tttgtcctat tcttatttca ttttactata gttttcgata gcaatttatt cttccaatac
    15961 acgaagaaaa acctccacat tcagtggagg caatctgttt tatcaataca attttaagtc
    16021 acgagggtca actgggaagg ttgggttgta tggattgtga cggagcttga agtgtttgac
    16081 atcttcaatg gtctgagttc cagacaattg cataactgtc ttcaattccg cattcaagtg
    16141 ttcaaagact tgacgcacac cgacactacc accgagagcc aagccataga tgacagggcg
    16201 tccaatagca accaagtctg ctcctgatgc caaggcttta aagacgtgtt gaccacgacg
    16261 aacaccagag tcaaagacaa tcggcacacg tctatcaact gcttctgcca cttcttgaag
    16321 cgagtcaaag gcagctggtc caccgtcgat ttgacgacca ccgtggttgg ttacccagat
    16381 accagaagct cctgcagcaa gcgaacgttc aacgtcctca cggcattgtg gtcccttgac
    16441 atacacagga agaccagagt attcagcgat aaattctaca tcgcgtggag acaagcgttg
    16501 tttagctgat ttgtaaacaa agtccattga tttaccagca ccttctggca ggtattcttc
    16561 aacaatcggc atgccaactg ggaagacaaa accattacgc ttatccactt cacgattccc
    16621 ccctacagta gcatctgccg tcaagacaat cgctttataa ccttcagcct tcacacggtc
    16681 catgatgtgg cggttgatac cgtcatcctt actaaagtaa aattgaaacc aatgaggtgt
    16741 cccttggagg gcttcagaaa tctctggaag gtcaacagta gagtaagaac tggttgtata
    16801 aagagaacca aactcatgca caccacgcgc agtcgccact tccccctgtt catttgccaa
    16861 tttatgagcc gcaacaggtg ccataatgat tggagaagat agtttttcac ctgcaaattc
    16921 aatctctgta cttggatttt ctacattgca aagtgtatga ggaacgatga gcttgtggtt
    16981 aaaggcacgg atattctctc ttaaagtgaa agtatcttcc gccccactag cgatatagcc
    17041 aaatgctgct ttaggaataa cttgttgcgc cattggctcc aaatcatagg tattgatgaa
    17101 gtctacatga ccttctgcat tgcttgtttt gtatgacata aactgtcctc ctcgattaag
    17161 taagcgtttg ctttttacta tacaactata ataactcttt tttcaatcat tttcaagagt
    17221 tttgcttaaa aaccatctta ttttttcata caatatacta gccctacttt ccttaaactc
    17281 taaaccttag tctatattaa gtgaatcgtt gagatttttt gctgccaggc atttgaacta
    17341 cttt
//
LOCUS       DBDESG010000053        16946 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.053, whole genome shotgun sequence.
ACCESSION   DBDESG010000053 DBDESG010000000
VERSION     DBDESG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.053"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..644)
                     /locus_tag="ACKB38_001694"
     CDS             complement(<1..644)
                     /locus_tag="ACKB38_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGL7980425.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGD"
     gene            complement(1189..3468)
                     /gene="pulA"
                     /locus_tag="ACKB38_001695"
     CDS             complement(1189..3468)
                     /gene="pulA"
                     /locus_tag="ACKB38_001695"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGL7980426.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(3580..5538)
                     /gene="ligA"
                     /locus_tag="ACKB38_001696"
     CDS             complement(3580..5538)
                     /gene="ligA"
                     /locus_tag="ACKB38_001696"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGL7980427.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSCEELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(5631..6809)
                     /locus_tag="ACKB38_001697"
     CDS             complement(5631..6809)
                     /locus_tag="ACKB38_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGL7980428.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLYSFLIIGLGVVALYFLALGYVRTHFEKLI
                     "
     gene            complement(6889..7752)
                     /locus_tag="ACKB38_001698"
     CDS             complement(6889..7752)
                     /locus_tag="ACKB38_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGL7980429.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLGKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(7755..9656)
                     /locus_tag="ACKB38_001699"
     CDS             complement(7755..9656)
                     /locus_tag="ACKB38_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGL7980430.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKSANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
     gene            complement(10082..10357)
                     /locus_tag="ACKB38_001700"
     CDS             complement(10082..10357)
                     /locus_tag="ACKB38_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGL7980431.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(10463..11302)
                     /locus_tag="ACKB38_001701"
     CDS             complement(10463..11302)
                     /locus_tag="ACKB38_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGL7980432.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            11444..12292
                     /locus_tag="ACKB38_001702"
     CDS             11444..12292
                     /locus_tag="ACKB38_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGL7980433.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(12385..13302)
                     /locus_tag="ACKB38_001703"
     CDS             complement(12385..13302)
                     /locus_tag="ACKB38_001703"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGL7980434.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKNYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            13782..13930
                     /locus_tag="ACKB38_001704"
                     /pseudo
     CDS             13782..13930
                     /locus_tag="ACKB38_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(14256..14549)
                     /gene="rpmA"
                     /locus_tag="ACKB38_001705"
     CDS             complement(14256..14549)
                     /gene="rpmA"
                     /locus_tag="ACKB38_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGL7980435.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(14566..14910)
                     /locus_tag="ACKB38_001706"
     CDS             complement(14566..14910)
                     /locus_tag="ACKB38_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGL7980436.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(14926..15240)
                     /gene="rplU"
                     /locus_tag="ACKB38_001707"
     CDS             complement(14926..15240)
                     /gene="rplU"
                     /locus_tag="ACKB38_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGL7980437.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(15276..15363)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(15422..16126)
                     /locus_tag="ACKB38_001708"
     CDS             complement(15422..16126)
                     /locus_tag="ACKB38_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGL7980438.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            16528..16827
                     /locus_tag="ACKB38_001709"
     CDS             16528..16827
                     /locus_tag="ACKB38_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGL7980439.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         5189 a         3608 c         2938 g         5211 t
ORIGIN      
        1 taatccccaa tttctgaacc acgacctgta atggtgttga aaacacctgc cggaatccct
       61 gcttcttcaa atgctttagc caacaagagt ccagaaatgg aaccttgtgt tggtggctta
      121 aacatgacca cattccctgc aatcaaggca ggtgcaattt tagaagcaga taaattaact
      181 ggataattaa agggagcaat cgctagcacg ataccaactg gttcacgacg gacaacagcc
      241 agtttgtttt tacttgttgc ctcaaaacca ccaccttcca ttgcttgtcc agtgatacgg
      301 agaccttcct cagcagcata acgaatcaag tctgctgtac gcactacttc tccaattgct
      361 gctttaatcc cttttgctac ttctttggca aggatagtac caatttcttc cttatcgcgt
      421 tctaaaatag ctgctgtttt atgcaaataa gccgcacgtt caactgctga taaagctcgc
      481 catgctggca gggctgcacg cgcagcttgc atagcctcat cagcttcagt ctgagtcatg
      541 gctggaactg tacccaattc ttcttgattg attggtgaat aaatcgtaat ttcttgttca
      601 gatgatttcc attttccatt tactaaattc tgatatcttg tcaaattttt gtcctcctga
      661 aaatgattaa gatatatttt accaaatttt cagaaaatca caaccctttt tgcaaaattt
      721 ttgagattat tttcacaaac ttgatttttc aaagtatact caataaaaat taaaaaaatc
      781 cactacgtca aggcgaggct aatgtggttt gaagaaattt tcgaagagcg tgaatgagta
      841 tcatctatag taaaataaaa aaactgaaca atttggttgg ggacagccaa accaatttct
      901 cacaatgttt cagaaacaag ggtgtgctat tccaatttca gcctactata actgtcatag
      961 attgctgaaa caaagtctag gtaaaagtct tcataataaa aagacctcct atcaagtgtt
     1021 caaaaacttt gataggaggt cttgttttgt gaaaatattt atcaaatttt ctatacaagt
     1081 gagctgttag ccaggttctt tctattcttt caatttcaat gaatggattt tttactaata
     1141 ctcataactg ggaatttgtc tgtgtaaaaa tagcgagata gatggtattt ataaaacact
     1201 caagacagct agactaatat catttaaaac attatcttct tttgagcgac tgttggttac
     1261 caacatagct aaatttcctg cattttcaaa ttgatagggt tctgatttag cattcacaac
     1321 caccaagagg tgttctttgc cgtgaacttc atagataagg tagccgctat gttcaatcgc
     1381 agaatgcaca aagacatgat ggtaaatttc atcatagcta gagtaagaaa aggcaccagt
     1441 ttttgtcttc aatcggatga cttgacggat aaactcaata ctgtcttgac gctcattaat
     1501 caagttccag ttcacttggt tcacactgtc aggagcatta tagctattca tcgcacgctc
     1561 tctatcatca tgggtcaact caccattttc accagtcgca accagtttgg tacgaccaaa
     1621 ttcttgaccg atttccataa aggccatccc ctgcatgagc agattcatgg ctgtggcagt
     1681 ttcgaccttg cgcatgattt gctctgaact ttggtctgga tgaagggttg ccaataaatc
     1741 gtgaagattg taattgtcat gggcttctac atagttaagc acctgatttg gatgtgtata
     1801 gcttcctaat tcacgacttc ctaggattgc tttagctaga attggctctg tcgcagcacc
     1861 actgacaaaa cctgacttga tagcaccata aacttctccc cctttgacag catcgcgctg
     1921 attgtcatta aagaaaccaa tatttggcat ctggtaggca ttgtccttct tggccttatc
     1981 ataaggggca agacctgttc ccatatccca tccttctcca tagaggataa tgttggagtc
     2041 gatttcatcc aagctttgac gaatcatctg catggtcttg acatcatgaa tccccatcaa
     2101 gtcaaaacgg aagccgtcaa tattatattc ctgcacccag tatagaagag aatcaatcat
     2161 atacttgcga aacatttcgt gttcactggc tgtttcattt ccaacacccg ttccattctg
     2221 gaaggtacca tctggattca tacgataata gtaatcaggg actgttgttt ggaatggtgc
     2281 atcaacaact gagaaggtat ggttatagac tacatccata atgactccaa tacccgcatc
     2341 gtgataagct tgaaccatca ccttcaaatc acgaatgacc tgagctggat catctggatt
     2401 agttgaaaaa ctagtttctg gcgcgttata gttttgtgga tcataacccc agttgtaggt
     2461 tacatttcca tcctcatcgt attctttatg acggtctgca attggttgca attgaacata
     2521 attgtagccc agcttcttga tgtaatcaaa agcagttgac tggccgtatt ggttaactgt
     2581 tccagcctga gcagcaccca agaaagttcc tcgaagatgt tcatctacac ccgatgtagg
     2641 tgatttagtc aaatcacgaa tgtgcatttc acagataact gccttacatg gattttccaa
     2701 gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt tcaacttgct tttctacatg
     2761 gctcagaata gctgaacgtt tgccatcagg gctggtcgcg attgtataag gatcacgtgt
     2821 cagtgtttgg tgatgaggga attggacttg atactgataa gtcttaccta ccaaatcttc
     2881 ttcaacatcc aaactccaga caccgattgt attgtcctta tgattataag agtagctatt
     2941 gcctcttttc atctcaaaag tcttccaaac gggtgcatca ttagcagctg attcataaac
     3001 gacaacttgc acttctgtcg ctgtaggtga ccagagagaa aaatgagcct gattgtcctc
     3061 tacacggcaa cccaattctc cttggtaacc ccaatgatga tcaaaactag cactgttaat
     3121 ggccttatca aaggcaaaag gattttgatt tttatagaaa ggactggcaa tagcaggatt
     3181 ttcagagtaa taaatcctat catcgccttc caaaatccag acctctgtta ataggggata
     3241 gtgattaaaa cggatagaat attctttact agtttgacct gtatgaacca caaaattcaa
     3301 gctttctata acatgtgaac ttgggtgttc aaagctaaat aaagctccaa aataatcttc
     3361 tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt acaaaggagc aagtgtcata
     3421 ttctccattc ttacgatggt aatgaatgcg catagggtag ttatacattt tttatttttc
     3481 ctttttactt tgtttctatt tcactaataa atttttgtca atctcgtctc aattaacaga
     3541 catagtcata ttctctaaac tctgttttta aacgatccat tacaaacttt ctagccatgc
     3601 ctcatctctg acctggatac caagttcttg tgctttttgc agtttacttc cagcgtctgc
     3661 acctaccacg acgaggtcgg tctttttaga aatactacct gtcactttgg cacccagact
     3721 ttcgagttta cttttagctt ctgagcgctt gagtcgttcc aattttcctg tcaataccac
     3781 ggtcaaacct gacaaggccg catccgctac taccgtctgt cctttatagt ccagattgac
     3841 cccagtttct ttcaattctc tgagcagaat ttcagagcct tctgtcgcaa aataagtctg
     3901 aagacttttg gcaatcacgc cacctagact ttcaatacta gccacttcct ctgaatctgc
     3961 ctgagacaga ttttcaattg aatggaaata ttgaagtaaa agctgactaa ccttgcttcc
     4021 gacatgacga attcccaaac caaataagag cttctcggca gaattttcct ttgatgcttg
     4081 gatagcctga tacagtttag cagcggactt ttccttaact ccctctaaaa ggaggaaatc
     4141 ctcttcttgc aaacgataaa tatccgccac atccttgact aaattagcag caaaaagctt
     4201 ctcaacaata gatggaccaa ggcctgtaat attcatagca tcacgagaag caaagtgaat
     4261 caagccttcc atgatttgag cagggcaacg cggattgata caacgtaggg ccacttcatc
     4321 ttcaaagtgc aacaagtcag agttacaact tggacagttt gtagggatat ctagtttttc
     4381 ttcagaaacc cgtttggact ctaccacacg taaaacggca gggatgatgt caccagcctt
     4441 atatacaatg accgtatcgt cttttcggat atctttttca gcaatataat ctacattgtg
     4501 cagggtcgca cggctaacag tcgtaccggc aagttgtact ggtgttagat tagcagttgg
     4561 agttacaaca ccggtacggc caactgtcca gtcaactgat aagagttgag cttctttttc
     4621 ttcggcaggg aacttgtagg ctactgccca ctttggagcc ttaactgtaa aaccaagttc
     4681 ttcttgactt gctaggtcgt tgaccttgat taccactcca tcaatatcgt aaggcagatt
     4741 ttcccgttcc tgtcctactt cttggataaa attccagatt tcatctatgt tttcagccaa
     4801 gattcgctta ggattgacca caaaacctag ttgttctagg tacttcaaac ccttttcttg
     4861 gctatcacga gttgaagggc tggcttcttg atagagaaac gttgcaagat tacgcttggc
     4921 aactactgct gtatccaact gacgcagagt tcctgctgcc gcattacgag gattagcaaa
     4981 ttcaggctct ccattttctt ggcgcgcttg gttaacttgg tcaaaggaag cgcgtggcat
     5041 gtaacattcc ccacgaactg tgatatctag ttcttctggc aaagtcaaag ggatgtcctt
     5101 aacacgcttg aggttttctg tgatattttc accaattgaa ccatctccac gtgttacccc
     5161 agcaaccaaa atcccctttt cataagtcag cgagatagat aagccatcga ttttcagctc
     5221 acaaatatag gtcggatgag ccacttcctt acgaacacgc gcatcaaaag catctagctc
     5281 ctcacatgaa aaagcatcct gcaaactata aagaggatac tgatgactgt atttttcaaa
     5341 accatctaaa accttgccac caacacgatg agtcggactg tctgctagca cttgctctgg
     5401 ataagcagtt tctaactcga ccaactcacg gtaaaggcgg tcatactcac tgtctgaaac
     5461 cgagggatta tcgctggtat agtactcagt cgcatagcga ttgagcaaag cgactaactc
     5521 attcattctt ttattcataa gaccatttta ccataaaaca agccctcctc acaaacgaga
     5581 agggcggaaa aaacacttag tttgaaatta tttttgaaac tcaagcaacc ttatatcaat
     5641 ttttcaaaat gagttcgaac atatccgaga gctaagaaat ataaggctac aactccaagt
     5701 ccaataatca agaaagaata aagatggaca cttggcaaga ctgtcataaa tccttttgca
     5761 ataggcataa atagaatagc taaggtaaaa attgtactca gtactcttcc aagaaattcg
     5821 ctctcaacct tggtttgtac ttgagtaaaa aagtgaatat taaaaatcgt cataaacaat
     5881 tcacaaacta aatttccaga aaaggaaaga aaagttggaa gtggtaatcc catcataaaa
     5941 actccgacac ctgtcaaagc cagtaaaatc aaaagattat aaatattagc tttaatttta
     6001 ctagctagaa gagccccaat gatggaacca atagccccca tagttaaaat acttgcatag
     6061 gctccttctg acccgtaaag ctgattcgaa aagggaagta gaaattcaaa agctgcaaaa
     6121 aagaaattaa cgctggaagc taccagcaaa aggaagaaaa tttcttgctg atgccagata
     6181 tagtgtaacc catccttgat atctacaaaa atatctctcc cagtaaaagc ctttttctct
     6241 tgaacttttg cttcctcttt tggaaggaaa gccactagaa caaaagcaat gaaaaaagtc
     6301 agcgagtcta gcagtagcgt catatggaga cttgcaaact gtaaaacaag gaaggaaaga
     6361 acaggagagc taacacctac aacctgcaaa accagctcta agcgagaatt atagatcaca
     6421 atctcatctt tctccaccac ttcagttatg atagctttat tggctgtgcg agaaaaggca
     6481 aaagcaatag cctgcacaat gttagcaaca atcaaagcgc caatcatcca gctatcattc
     6541 cttatgaaag aaatagccag acaaagaatc ccacaaacaa gatctgccgt cattaaaatc
     6601 ttacgacgag aaaaacggtc tgaaataact ccgccaaagg gattgacgag aatagatgtg
     6661 acgagctcag aaatctgata cattcctaaa actgtctgtc ctatagtccc catagaagcc
     6721 aaccagacac tatttccata atcatagagc atatttccca ttttattgat agccccacgg
     6781 ctaatcaact gcactgcata gcgattcata ttaaagctcc tctcaaattt tgaaactatt
     6841 gtatcaaaac cgaaaggagc tttttatttt ttcccttatt tgggaaaatt aacttttgac
     6901 aaatttttcg tagtgttcct gataataggc tacttgctct ggaagaccta acacatcaaa
     6961 aatatgcatg gcctcttgca tctgcttaca gccttcttta cactgtcctt tttgatataa
     7021 ggcaaaacct tttaaataat ggaaaacatt acgctcataa agcttaatac ctttgtcaat
     7081 aatcttctct gtataagcct caaaatagtt ggcattataa aaagaagaat gctctaaaca
     7141 atgctggtaa caattgaggg ccaaaatcaa cactaatctc ttatggcgac taatctcttg
     7201 gtaaaattcc tccctctcca taacttctct accaatccga gtgacatagt ctacatcgta
     7261 gaaactatag aggttaccga aaagaatcaa ctcatacatg gtccattctt ctgttttgaa
     7321 gagataatct gctaccttac ccaaatcatc ctgcttcata tcataactcg catctctttg
     7381 acaaatcaga ccttgtagca aaatccagtt cagctcaaaa taaaggggag tcgtcgaact
     7441 cttagacttt tcaagttgtt ctctttgaag cttttgaaaa cctgcaatat cgtttgaata
     7501 gtaaagtggg ataatctgtg ccatcataga cacatgttca tgattatgaa aattccttgc
     7561 cttatccatg aaattttcga ttgttacatg aatgttatcc aaaatctcaa agaaacggga
     7621 gactgccagg tcagactccc caagctcaaa gcgagataac tgagaggtag agcaggattc
     7681 gcctgctgct tcctttaaag aataatttcc acttgttcga aattcacgaa atacttttcc
     7741 aagatgttcc atctttacac ctgctctgat aattcttccc actcaagcat agcttcttcc
     7801 tgacgatggc tgattttgtc cagctcagcc tgtaattcca tgagtttgtc ggcatcgttt
     7861 gtttccaaca tttgttcaga aatggcttgg ctttgacttt ctagctcttc aatttcagct
     7921 tctagacttt cgatttgtcg catgagtttg cgaacttctt tttgactttc tttctgggcc
     7981 tgatagtcat tgactggact tgcttccttt gcttgattgc tagttgaagc ttcctcagtc
     8041 tgactcattt ctgctgttgc tttcttctca acatagtagt cgtaatctcc aaggtagaga
     8101 gttgaaccat tctcagacaa ttccaaaaca tgagttgcca cacgattgat aaagtaacga
     8161 tcatgactga caaacagcaa ggttccatca aagtcaatca aggcattttc tagcacttcc
     8221 ttactatcaa tatccaagtg gttggtcggc tcatccagaa tcaaaaagtt attgttttcc
     8281 atagacaatt tagctaaaag caaacgagct ttttcgccac cagatagcat gccgactgat
     8341 tttttaacat catctcctga gaaaaggaag gctccaagac ggttgcggat ttcaacttct
     8401 ggtgtcagtt tgaaatcatt ccagagttca tccagcaccg tattacttgg tgtcagcttg
     8461 ctttgggttt ggtcatagta accaacctca acattagcgc caaagcgctt ttctcccttg
     8521 ataaaaggaa tctggtccac aatagacttg ataaaggttg acttgccgat accatttgga
     8581 ccaacgatag cgacagcatt catcttacga agatctaggt taatcggttg tgacaagact
     8641 tccccgtcat agccaacagc tgcattttca acagtcaaaa caacattgcc cgacgttttt
     8701 tcagactgga aggtcatgtt ggctgatttc ttgccagctt caggcttgtc caaacgttcc
     8761 attttttcca gttgtttacg gcgagattga gcacgtttag tcgttgaagc acgaactaga
     8821 ttgcgattga caaagtcttc cagagcagcg atttccttct gttgcttttc atagtttttt
     8881 gcctcagtaa ctagcttttg ctccttcaat tcgacaaaac gagagtaatt ccccacatag
     8941 cgatccaagg aatgcttggt caaatctagc gtaattgtcg caaccttgtc caagaaataa
     9001 cggtcgtggc tgacgataat gagggcaccg ctatagttta ccaagtaatt ctctagccag
     9061 gcgatggttt caatatccaa gtggttagtt ggctcgtcca agaccaagag attgggcttt
     9121 tcaaggagca ttttggcaag tgccaaacga gtattttgac caccagaaag ctcagcaatt
     9181 ttcatctgcc acatagactc gtcaaacttg aatccattca aaatcgctcg aatatcagct
     9241 tcataggtaa agccacctgc ttggcgaaaa ttctcagata agcggtcata atctgacatc
     9301 agtttatcca aatcctcacc agacttttca cccatctcca gctccatctg acgcagttgt
     9361 ctctccgtcc gacgcaaatc attaaagaca tgaagcattt catcgtagat ggtattttca
     9421 gactcaaaac ggctatcttg ggctaggtaa gacagagaaa tatctttttt cttattgatt
     9481 tctccgctag ttggctcctc ttctccaact aaaatcttca aaagagtaga cttacctgca
     9541 ccatttttcc caacaagagc aatccgatct cgttcatcaa cctgcaggtt gatattatcg
     9601 aaaagaacct ctcctgcaaa agaacgttca attttattag cttgtaaaat aatcatacaa
     9661 gtagtatagc atgtttccct aaggcattca agataatcgt aagtctttta gtacaacttt
     9721 tataacataa aataaactaa attatgtata ttttatatta gattacttca ctatcttgtt
     9781 ggattttcta accagctaat cttgtttcaa atagttatcg cacaagtcta ttatttaatt
     9841 cttttcatca tttacgtacg tatagcagat tgaaataaga tgagaacaaa tcgattggga
     9901 aagtaaaatt aatttctata aatgttttag caattgtttc gtactatttt agattcagtc
     9961 tactatatac aatattttcg gaacattcaa ctttttaact ctatttatta ctagatttca
    10021 taattaaaaa acctactgac caagctagaa agcttgatac aataggcttt ttaaagactg
    10081 attatttaac agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg
    10141 caattgtcat ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa
    10201 cttcaaagtt accaaaaccg atcaattgaa ctttttcacc agctgcaaga tagtcagcta
    10261 ctgctgcaaa tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt
    10321 ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt
    10381 tctaattaac aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat
    10441 ttcttcccat tttatttatt ttttaggagt ggtaacgtat caaaatagcc caagcgttct
    10501 cacccgtgtg agtttgaata atggaacccg tttccaaaac agaaattggc ttttcaacat
    10561 aagcttgtaa gctttctttc atctcttttg cccaatcatc actaccagaa tatgaaattc
    10621 caatctctgc tacagcacgt tcagaaagcg atgttatcaa ctcatctaac cattttttaa
    10681 atgttttagt tccacgacct ttaaccattg gctgcaattc atggtctttc atttgcatga
    10741 cagcacggat attgagaaga gagctcaaca agccagttac acggctaatt cgtccacctt
    10801 tgacaagatt ttccaaagtt gaaacaccaa tataaagctc tgtatggttt ttaacctctt
    10861 ctacatgaga taaaattgcc tccatatctt taccttcttg agctaacttc gcagcctcaa
    10921 caacttggaa tttcagggct tggtcagtga aggaactatc aacaacagtc acatctgcag
    10981 tagataggct agcaccttgg cgtgctgctt ctaccgtacc cgaaagagca tgggacatat
    11041 gaatagcaag aatctggcca ccatctttgc ataggtcttc aaaaatctca gcaaagacac
    11101 ctacaggtgg ctgacttgtt ttcggaagat tcttactttc ttgcatcaac tgaagaaatt
    11161 taccttcttc tttcaaatcc gcatcagaat aaacaacatt atcaatcatt acagataatg
    11221 gaacaattgt aatatctaat tgctttacta gttcaggttc aatagtaaca gatgaatcgg
    11281 ttacaatctt aatttttgtc atagtatcaa tctttctatt ttaggattca gattggtttc
    11341 cttacttcta attatatcaa aaaaaagatt aaaaatccta atggagtcaa tcaaattttc
    11401 cgtaaaattt gatataatca acttataaga aaagaggtgt cctatgatta aaaaaattta
    11461 ccccattttt accattttac taggtgctgc tatttatgct tttggactga cttattttgt
    11521 agttccccat catctctttg aaggaggggc gacaggcatt accctcatca ccttttatct
    11581 ttttaaaatc cctgtttccc tcatgaacct gctgattaat attccccttt tcatcctagc
    11641 ttggaagatt tttggagcca aatccctcta ttctagttta ctaggaacct tagctttgtc
    11701 cggctggtta gctttttttg agcatattcc ccttcatatt gatcttcaag gtgatttact
    11761 aatcacagcc cttatagcgg gaatcctatt gggaattggc cttggaatta tttttaatgc
    11821 tggaggtaca actggcggaa ctgatattct agctcgtatt ctcaacaaat acactcatat
    11881 atccatagga aaactgctct ttatcttaga tttttgtatt ctcatgttga ttctcctaat
    11941 cttcaaggat ttgagattgg tttcctacac gcttttgttt gattttattg tttctcgtgt
    12001 tattgatttg attggtgaag gaggatatgc cggcaaaggc tttatgatta tcacaaaacg
    12061 tcctgaccaa cttgctaagg cgattaatga tgacctcgga agaggtgtta cttttatttc
    12121 tggtcaaggc tactatagta aagaaaattt gaaaatcatc tactgtattg tcggaagaaa
    12181 tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat cctcaagcct ttataactat
    12241 tacagaagcc catgaaatcc taggagaagg cttcaccttt gaaaaagaat aaaaagaggt
    12301 aatgtcgtga cctcaaaagt tagactaaat catctatctt ttgggttaca gacaacctct
    12361 tttttatttt atttactcaa gctcttaaga ccaattccga gttacttctt catcagcctt
    12421 taactgatcc actaattggt caactgagtc aaatttggtc atatctcgaa tgcgatcaag
    12481 ccaataaacc atgacggttt ccccataaat atcttgatta aaatcaaaaa tattgacttc
    12541 aaaacgtgct tcttctccat caaaggtcac atttttcccg acactagcca tagcacgata
    12601 cttctgtctt tgaatctcaa catcaacaac ataaacgcca tctgctggca tataagtacg
    12661 gtctaaaagc actaaattcg ctgtcggata accaattgta cgaccacgag cattaccatg
    12721 aaccaccata cctcttgatg gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt
    12781 tccatctaaa atagcttgac ggatacgagt tgaactaatc tttcctttct catcttctac
    12841 aggtggaaca atgataactt ctccatcaaa gtaattcttt aaatcttctg ctgttttttt
    12901 gtcagaacca aatgtataat caaaacctgc aacaataatt ttggcattca tagccttgat
    12961 ataagttgca aagaattctt gtgcagtgag actagcgaat tgactactaa aatcaaggag
    13021 atataattct tctacacctt cgcgctttaa ttttctttca cgttcagcag ggttcaaaat
    13081 atgcaaaaac aaatctggat gataaggctc taaagcgatc tttggagatt cattaaaggt
    13141 cataacgacg ataggcaaca aatcctttct cgcagccttg ttggcaacac gaaataattc
    13201 ttgatgcccc ttatgtatgc catcaaaata gccgagaaca acgactgaat cagatggtgt
    13261 gccaatatct ttttggtttt ttataggaat agtaataatc ataaaataat tatatcatag
    13321 cgatagctat ttctggaaca gaaaatctga aatgttgttt ttttcacatg aagtgtacct
    13381 gttttcaaaa agcactttat tctatcgttg cttaactatg aactttgcaa tattcttctc
    13441 aaaaacttgt aggacatctt caaaattttg caaggagtga ttagacttgt tcggtaacca
    13501 taaagtgtca tactatgctt atgtatgaaa aagcaatgca actaactcct gagaacttta
    13561 aattactaat tggtgccgaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa
    13621 atttacaaaa tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt
    13681 gaagcaagtc actttcccta atgacaaaga aaaaagccaa aacagcaaag aaaaactaga
    13741 tgactgcttt ccaacaactc ccatctagtg tgcttcagac tgggctattt ttctctccat
    13801 ctgttagctt ggattctcag accgtttcag ctaaagaata tcttttccct tatcagaagg
    13861 aacggctcaa gccattcaga caagtgaagg gacgacaagc caatatttga aaccagatag
    13921 cagttcttat agtcaattga aataaaatct gaagaaatcg agtaggaaac tcatatcaat
    13981 gtttaacagt gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag
    14041 ttagccgcag gctgtacttg agtacggcaa ggtgaaattg acatggtttg aatttgattt
    14101 tcgaagagta tcaatccact atacatatta aaaagtatgg gtaccaaaac aaaaaaacag
    14161 gctctccgaa aactcggaaa gccttatttt aagctgtttc tagcttcctc acctttacat
    14221 ttcaaggttc gggataaaag gttcaatgga cctttttatt tagcgattgg gtaaacagaa
    14281 acttgttttt tatcgcgtcc tttacgttca aagcgtacta cgccttcaac tttagcgaac
    14341 aaagtatcat ctccaccacg accaacgttt acacctggat agatgtgtgt accacgttga
    14401 cggtaaagga ttgatccacc tgttacagtt tgtccgtcag ctgctttagc tccaagacgt
    14461 tttgcttgtg aatcacgtcc gtttgatgta gaacctccac cttttttgtg ggcgaaaagt
    14521 tgcaagttgt taagagtcat ttttaacata atgttttcct ccgtgttagt tttctgtgat
    14581 aactctggtt tggacgaact cagaatagtt ctccgataag tttgccatac ctaagaaaaa
    14641 tgattcaaag aataactggg tcatttctct ctggtgtgaa ggaagatctt ttggtatttc
    14701 aaccatcaga tagccacctt catcttcgtt taattctagg attggttcat agcctgcaaa
    14761 tttctcaata gaattgataa agttaatggc aagcgtagaa accgatgcac acacgacatc
    14821 taagccgtat tcgccactct cggcgtgtcc agtaatttcc gcactcctca gctcgccatc
    14881 ttcggctctc tcaaagactg cctgtatcat gtgttctcct taaaattaag cgttgattgc
    14941 gttgatgaca acttttgtat atggttgacg gtgaccttgt ttacggtggc tacctttttt
    15001 aggtttgtac ttgtaagtaa ccactttctt ttgttttcct tgtttttcaa cagttccaac
    15061 tacagtagct ccagcaacaa gtggagttcc gacaacagtg ttttcaccac caacaagaac
    15121 aacttcgtta aaagtaactt cttgaccagc ttcaacgttc aatttttcaa cgtaaactgc
    15181 ttgaccaact tcaactttaa cttgttttcc gccagttttg ataattgcgt atgtgctcat
    15241 tatgcacctc ctatgatttt tatgggtttc cccgattttt tgtgaagact cgcctagcat
    15301 cgtgggacga accacttaaa cttgaataat caagcaacga tgttcgtgcg gttgcacagg
    15361 atcgtgcata gtcaactctt caagtatagc atatctccta ttttcttaca agtaataaca
    15421 cctaaaatga agctttttct tttacttttt tctgccaaga ggcaaaaagc atgctgaggt
    15481 aaaaaacgct catcataata ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca
    15541 aataggctga aaagacaacg ccaaggacaa aactactaag caggctaaca aatatgaatc
    15601 cttcacgcaa aaaaggagtg tgcttggttc ggaaataatc tccaaaagcc agcatggtcc
    15661 gtttgatatt ccctgtcata aaagcgttat tataggcaat acccgacact tctccaaaag
    15721 cagttgtcac cagtcccata cagaaggcca agggcggcac tagatagata ttatccacag
    15781 tttgcggcac aaaagcaata atgattgata agattgccaa gggaatcaag gacagaatag
    15841 gttttttcac aattctcaat ttttccttat aaatcgttaa taaaaagact cccatcataa
    15901 acgctagcaa ggtgagaacc ttgtccctaa catccgaaac attattttta attaattcta
    15961 ctgaaagaaa gacaacattt ccagtttgtc cagctacaag ggtattcccg cgaacaataa
    16021 aagtgtaagc atccacatat ccagcacaaa acgtcaaaaa aagtgctaac cttttagact
    16081 gacgtgatat ttttcttata ggtaataacc tcattttacc tcccattgta ttttctctta
    16141 gaaatattgt accattttct ttctaaaaaa tcgtaggcta ccatttagat tttactatta
    16201 gcataaaaat aataatagac aactatttat ccaaaaatag atagatgtaa catgtttgca
    16261 aacaaagcat acgaaccttt agtaaaatca tttccatgaa actagaatag agccctctta
    16321 gcaaaaatca ttattttaat ttatttctaa tcactccttg acataaataa ctctcaccaa
    16381 taaaagacta tgtcttaaaa aaatggtata ataaaatcaa tacttgggct tgatggctat
    16441 gctactaata acaattagga gagaaaatca ggcacttgtt aacaacaagg attatcccct
    16501 tgagatgaaa ggaactttag aaatcttatg atgaacatgc aaaacatgat gcgtcaagca
    16561 caaaaacttc aaaaacaaat ggaacaaagc caagctgaac ttgctgctat gcaatttgtt
    16621 ggcaaatctg ctcaagatct tgtccaagcg accttaactg gcgataagaa agttgtcagc
    16681 attgatttca atccagctgt cgttgaccca gaggaccttg agactctttc tgatatgacc
    16741 gttcaagcca tcaactctgc tcttgaacaa atcgatgaaa ctaccaagaa aaaactgggt
    16801 gctttcgctg ggaaattacc tttctaaaaa caaggagcta gaacaatgct tgtcgataac
    16861 aaaggctaag aaaggtgcaa aaatgactct ataatatttg tagtgggtaa atcccctatg
    16921 gatattatgg agcctatttt tgtgta
//
LOCUS       DBDESG010000054        16714 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.054, whole genome shotgun sequence.
ACCESSION   DBDESG010000054 DBDESG010000000
VERSION     DBDESG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.054"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..239
                     /locus_tag="ACKB38_001710"
     CDS             <1..239
                     /locus_tag="ACKB38_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGL7980440.1"
                     /translation="ALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAIA
                     ALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            232..708
                     /locus_tag="ACKB38_001711"
     CDS             232..708
                     /locus_tag="ACKB38_001711"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGL7980441.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            705..1553
                     /gene="pheA"
                     /locus_tag="ACKB38_001712"
     CDS             705..1553
                     /gene="pheA"
                     /locus_tag="ACKB38_001712"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGL7980442.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESCPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            1550..2824
                     /gene="psr"
                     /locus_tag="ACKB38_001713"
     CDS             1550..2824
                     /gene="psr"
                     /locus_tag="ACKB38_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGL7980443.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            3103..3948
                     /locus_tag="ACKB38_001714"
     CDS             3103..3948
                     /locus_tag="ACKB38_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGL7980444.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGDL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            3938..5041
                     /locus_tag="ACKB38_001715"
     CDS             3938..5041
                     /locus_tag="ACKB38_001715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGL7980445.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            5043..6029
                     /locus_tag="ACKB38_001716"
     CDS             5043..6029
                     /locus_tag="ACKB38_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGL7980446.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            6045..7478
                     /locus_tag="ACKB38_001717"
     CDS             6045..7478
                     /locus_tag="ACKB38_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGL7980447.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            7507..8541
                     /locus_tag="ACKB38_001718"
     CDS             7507..8541
                     /locus_tag="ACKB38_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGL7980448.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESDKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANNLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            8741..9478
                     /gene="mecA"
                     /locus_tag="ACKB38_001719"
     CDS             8741..9478
                     /gene="mecA"
                     /locus_tag="ACKB38_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGL7980449.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEVKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            9629..10915
                     /locus_tag="ACKB38_001720"
     CDS             9629..10915
                     /locus_tag="ACKB38_001720"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGL7980450.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            10917..11786
                     /gene="thrB"
                     /locus_tag="ACKB38_001721"
     CDS             10917..11786
                     /gene="thrB"
                     /locus_tag="ACKB38_001721"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGL7980451.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            11862..12008
                     /locus_tag="ACKB38_001722"
     CDS             11862..12008
                     /locus_tag="ACKB38_001722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980452.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVADRTDEVSNHTYGKVTLTWFEEI
                     FEEY"
     gene            12037..12975
                     /gene="msrB"
                     /locus_tag="ACKB38_001723"
     CDS             12037..12975
                     /gene="msrB"
                     /locus_tag="ACKB38_001723"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGL7980453.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            13033..14757
                     /locus_tag="ACKB38_001724"
     CDS             13033..14757
                     /locus_tag="ACKB38_001724"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980454.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGSAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VLVAILTIVIVGLSRLVNPFYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDSLVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            14759..16510
                     /locus_tag="ACKB38_001725"
     CDS             14759..16510
                     /locus_tag="ACKB38_001725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980455.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYFSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
BASE COUNT         4982 a         3094 c         3651 g         4987 t
ORIGIN      
        1 acgccttaaa tagtatggga gcagatatta ctcctacggc agatgggatg attatcaaag
       61 gaaaatcagc tcttcacggt gctagagtca atacgtttgg tgaccaccgt atcggcatga
      121 tgacagctat cgcagccctc ttggttgcag atggagaggt ggagcttgac cgtgcagaag
      181 ccatcaatac cagctatcct agtttctttg atgatttgga gagcttgatt catggctaag
      241 gtgttattag ggtttatggg ggctggaaaa tcgactattg caagaggctt ggaccctaat
      301 taccttgata tggatgctct gattgagaat cgcctaggta tgtccattgc gaattttttc
      361 gctgaaaagg gagaaacagc ctttcgtcag gtagaatcag aagtcctagc tgatttacta
      421 caaacagacc aagtcgtgtc aactggagga ggagtggtta tttctcagag aaatcgtgac
      481 ttactcaaga ctaatacaga taacatctac ctgagagcag attttgaaac cctctaccaa
      541 cgtatcgcag ctgataagga caatcagcga ccgctttttc taaataatag caaggaagaa
      601 ctagtagcta ttttccaaga aagacaggct tggtatgagg aagtggctag tcgggttttg
      661 gatgtgacca agctaagccc agaggaaatt atagaggaac taagatgaaa attgcttatc
      721 taggtcccaa gggatcattt tcacaccacg ttgtgcagac agcttttcct catgaggaat
      781 tgcaggcttt tgccaacatt acagatgtca tcaaggctta tgagcaagga ttagtgaact
      841 attctgtggt gccagttgaa aattctattg agggtagtgt tcatgaaacc ttggactatc
      901 tttttcatca ggctcacatc caagcagtag cagaaatcgt tcagcctatt catcagcagt
      961 tgatggtggt tccaggccat actaagattg aaaagatttt ttcacatcca caggccttgg
     1021 ctcaaggaaa gaaattcatc gatgaacaat atccagaggc tcaaatcgag gtaacagcta
     1081 gtacagctta tgcggcccgt tttatttccg aacatccaga ccagcccttt gcagcagttg
     1141 cacctagaag ttctgctgaa gaatatggat tggaactgat tgccgaggat attcaggaaa
     1201 tggaagccaa tttcacacgt ttctgggttc taggagctga aaagcctagt attcccttgc
     1261 aagcacaaac tgaaaagatg agtttggcct tgacattacc tgacaacctt ccaggtgcac
     1321 tttataaggc cctgtcgacc tttgcttggc gaggaattga cttgacaaaa attgaaagtt
     1381 gtccactcaa gacagcactg ggtgaatact ttttcattat cgatgtggat tataccgata
     1441 aggacttggt ccactttgcc caaaaagaat tagaagcgat tggaatccag tataaaattc
     1501 tgggtgccta tcctatttat ccaatatcag accatggaaa ggagagaaga tgagtaaaga
     1561 aaatccctta agtcatcatg agcagttgcg ttatgattat ttgctaaaaa atattcacta
     1621 tctcaatgag agagaaaaaa atgagtttgc ctatttgcaa gaaaagctaa ctcttgctag
     1681 gggaaatagt agctctagct tggaacaaga aagagaagag caggttgact taccaagcta
     1741 tgcgaaccgg agtcgctcac aatccaaatc acaagcactc tctttccctc caaaaaagaa
     1801 aagacggaag ctccgtctta aacgaatttt tatggtgatt ttttcacttt tggtctgtgt
     1861 ggctttggcc atggtattca tgtttttgcg tggttaccaa gatgctagcg caaagaaaac
     1921 tgctgatgcc cgggcagctc aagtagaagt ctttaatggt caggacacta gagatggagt
     1981 taatatttta atcatgggta ctgatggtcg aatcggccag aacagtgttg agacacgaac
     2041 tgactctatt atggtattaa atgtcggggg ctcagataag aaaatgaagc tagtcagttt
     2101 catgcgtgat aatttggtct atatagatgg ttatagtcaa gtgattaatg gtagaaaaca
     2161 gacagataac aagttaaacg tagcctacga gttaggagaa caagaggggc aaaaaggggc
     2221 agaaatggtt cgccaagtct tgaaagataa ttttgacttg gacattaagt actacgcctt
     2281 ggttgatttc caggcctttg ccacagcgat tgacacgctt ttccctgatg gggtgacgat
     2341 tgatgctcaa ttttcaacat tgaatgggcg tcccctaaca gaagctacag tcggagatga
     2401 tttatacgct accgagactg agtctccaac ccaaaccatt aaagtcggaa aacagcagat
     2461 gaatggctcg accctgctca attatgctcg tttccgtgat gatgatgagg cagattacgg
     2521 ccgtacaaaa agacagcaac aagttttaac agcaatttta gagcaaatta aagatcccac
     2581 taaacttttc actggttctg aagctcttgg aaaggttttt gctatgacct caaccaacgt
     2641 accctatact ttcctcctaa caaacggttt atctgttttg gatggagcaa aaaatggtat
     2701 tgaaaaattg acgattccag aactgggtga ctgggtagat gcctatgatg tttatggggg
     2761 cttgggcctg ctggttgatc aaaacaaata tcaaaccaag ctcgctcaaa tgggcttaag
     2821 ataagataat actcaatgaa aatcaaagtg caaactagga agctagccgc aggctgctaa
     2881 aaacacagtt ttgaggttgt agataagact gacgaagtca gttacctata cctacggcaa
     2941 ggtgaagctg acgtggtttg aagagatttt cgaagagtat aagacagaaa aatcaaagtt
     3001 tgatagctat ttggatagca gtcaggcttt gattttttac agtctgcaat agattttcac
     3061 cccttgtttg tggtataatg aaacgatacg atagaaagaa taatgaacaa tatgactgat
     3121 ttaaaagcaa ttcaggctcg tagtctggag atggctgaat attttgtggc cttttgtaaa
     3181 gaacatgatt tgctctgtta tctctgtggc ggaggcgcta ttggtgccct tcgaaacaag
     3241 ggatttattc cttgggacga cgacctagac ttttttatgc ctcgtaaaga ttatgagaaa
     3301 ttagcagaat tatggcctcg ttatgcagat gaacgttatt tcttgtcaaa gagtcacaag
     3361 gattttgttg atcgtaatct ttttattacc attcgtgaca agaaaaccac ctgtatcaag
     3421 ccttatcagc aggatttgga tttgccacat ggtctggcct tggatgtttt gcctttggat
     3481 tattatccga aaaatccagc tgagcggaaa aaacaggttc gttgggcctt gatttattca
     3541 ctcttttgtg cgcaaactat tccagaaaag catggtgatc tcatgaaatg gggaagtcgc
     3601 attttactgg gtttgactcc aaaatctctc cgttatcgca tctggaaaaa agctgagaaa
     3661 gaaatgacta agtatgattt ggctgattgt gatggcatta cagaattatg ctcaggtcct
     3721 ggctacatga gaaacaagta cccaatcaca tcttttgaag acaatctttt cttgccattt
     3781 gaaggaacag agatgcctat tccaatcggc tatgatgtct atctcagaac tgcttttggg
     3841 gattatatga cgcctccacc agcagacaag caggtaccgc atcatgatgc tgtcatcgct
     3901 gatatggata agtcttatac agaatacaag ggagaatatg gtggctaaga aaaaaatctt
     3961 attttttatg tggtcttttt ctcttggagg tggtgcagag aagattctat caaccattgt
     4021 ttcaaatctg gatccagaaa agtatgatat tgatattctt gaaatggagc actttgacaa
     4081 gggatatgaa tctgttccaa agcatgtacg cattttaaaa tcccttcaag attatcgcca
     4141 aaccagatgg ttacgagctt ttttgtggag aatgagaatt tattttccaa gactgactcg
     4201 tcgtttgctt gtaaaagatg attatgatgt tgaagtttct tttaccatta tgaatccacc
     4261 actgttgttc tctaaaagaa gagaagtcaa gaagatatct tggattcatg gaagtattga
     4321 agaacttctt aaggatagct ctaaaagaga atcacataga agccagttgg atgctgcgaa
     4381 tacaattgta gggatttcaa aaaagaccag caattctatc aaggaagttt atccagatta
     4441 tacttctaaa ttacagacaa tctacaatgg atatgatttt cagactattc tagaaaaatc
     4501 tcaagagaag atcgatatcg agattgctcc tcaaagtatc tgtactatcg gacggattga
     4561 ggaaaataag ggttctgacc gtgtagtgga agtgatacga ttattacacc aagagggaaa
     4621 aaactatcat ctctatttta tcggggctgg tgatatggaa gaggaactga aaaaacgagt
     4681 caaagagtat gggattgagg actatgtaca tttccttggt tatcaaaaaa atccttatca
     4741 gtatctatct cagacgaaag ttcttttgtc tatgtctaaa caagaaggtt ttcctggagt
     4801 gtatgtggag gccttgagtc tgggactccc ttttatctct acggacgttg gaggggctga
     4861 ggaattatcc caagaaggac gatttggaca aatcattgag agcaatcaag aggcagctca
     4921 ggcgattact aattacatga cttctgcctc aaactttgat gtcgatgagg ctagccaatt
     4981 cattcaacaa tttacaatta caaaacaaat cgaacaagta gaaaaactat tagaggagta
     5041 gcatggaaac tgcattaatt agtgtgattg tgccagtcta taatgtggcg cagtacctag
     5101 aaaaatcgat agcttccatt cagaagcaga cctatcaaaa tctggaaatt attcttgttg
     5161 atgatggtgc aacagatgaa agtggtcgct tgtgtgattc aatcgctgaa caagatgaca
     5221 gggtgtcagt gcttcataaa aagaacgaag gattgtcgca agcacgaaat gatgggatga
     5281 agcaggctca cggggattat ctgattttta ttgactcaga tgattatatc catccagaaa
     5341 tgattcagag cttatatgag caattagttc aagaagatgc ggatgtttcg agctgtggtg
     5401 tcatgaatgt ctatgctaat gatgaaagcc cacagtcagc caatcaggat gactattttg
     5461 tctgtgattc tcaaacattt ctaaaggaat acctcatagg tgaaaaaata cctgggacga
     5521 tttgcaataa gctaatcaag agacagattg caactgccct atcctttcct aaggggttga
     5581 tttacgaaga tgcctattac cattttgatt taatcaagtt ggccaagaag tatgtggtta
     5641 atactaaacc ctattattac tatttccata gaggggatag tattacgacc aaaccctatg
     5701 cagagaagga tttagcctat attgatatct accaaaagtt ttataatgaa gttgtgaaaa
     5761 actatcctga cttgaaagag gtcgcttttt tcagattggc ctatgcccac ttctttattc
     5821 tggataagat gttgctagat gatcagtata aacagtttga agcctattct cagattcatc
     5881 gttttttaaa aggccatgcc tttgctattt ctaggaatcc aattttccgt aaggggagaa
     5941 gaattagtgc tttggcccta ttcataaata tttccttata tcgattctta ttactgaaaa
     6001 atattgaaaa atctaaaaaa ttacattaga acgggggtga ccaagtgatt gatgggaaac
     6061 gattattatt tagtttgacc atagtcagtt atgccttgac gctagtaagt ggaattgtgt
     6121 atctgtttaa taataataat gttagcttac tttctacttt attgttcttg ttggttagta
     6181 gcttaattgc ttgttggaat gatatcaagt attacttaat ccattttatt ttctatttaa
     6241 ccatttttgt atttctggta tcaagaccga ccattgatta ttttagggat ggtgctttgg
     6301 atacctatca tccaatagcc tatcgttttg cctttatagt tgtcatgatt tcgattctgg
     6361 gcttgaccac aggaggcatt ctggctcgtt acttcatagc taggaagaaa ataaaagtag
     6421 caaatatagg aaattctcta aaagaggttt atatcaagcg gttacgcttt gtatcactag
     6481 gagtttttct tctaacttat cctttctatt tcattcggtt atttgaacgg ctcttgtatc
     6541 gtttgcagac ttcctactat gcctactatg caaattttga aagtaaactg ccttatttta
     6601 cctacatttt gtctaccttt acggtctatg caatgtgtat gtatctggca accaagccaa
     6661 agaaattgca ggccacagca gtgcttgtct cctttattgc agctaatact attcatttgg
     6721 caattgggac acgaaatccc tttattttaa gtattttatt tgcttttgtt tattacttta
     6781 tgcgggagca aactgaaaaa ggaaaatgga ttgggtttaa agaaaagtta gcgatttttg
     6841 taggttctcc tattctcatg ttagcgatgg gagtactcaa ttatgtacgg gataatgtcc
     6901 aagtttccca tacaggtttc tgggatatct tacttgactt tatctataaa caagggacta
     6961 gttttggtgt tctggctcga ggttttctat ttaacagtag cctcccttac cgagatttcc
     7021 gtaattttac ttttggtcct gttcttgatt attttgcaag ggggagtttg ggagccattt
     7081 tcggaggaaa agcctttgaa catacaacca atagtgtgga actagctatt gatagtaata
     7141 gttatgccca caatctatcc tatcttgtct tgaacaagga atacttgaaa gggcatggta
     7201 tcggaagtag ttatatcatg gagttgtata ccgactatgg tatgattgga gtctttctgc
     7261 ttagtttctt actcggcgta ttatttatag ccatgctgca agtagcctat cgctcaagga
     7321 caatcctatt tgctttatcc ctactcatct tgaataatct attctttatg ccaagaagca
     7381 gcttttcaga aagtttcttc aatttattta caatgcaatt ctggggaatt gttcttgtga
     7441 ttatatttgt agcaaaaatg cttacaaagg aaaactagta tctactaaac aaaggagaaa
     7501 aaaatcatgt ttgaacatta ttcagtagct gatttgtttg caaatcttta taaaaagcga
     7561 aaagcaaata ttttagctct catcgcttta tttgctctca ttgctgtacc atttacaatt
     7621 aaagcagtta ggaataaaaa cactgtcaaa gatacaacaa gttattcaac ttatcttatc
     7681 tataaaatca ctcctccaaa agagtcggac aaaacgattt tgaatcatca aattggtggt
     7741 tatagtgatt tttatgggaa attgattgat ggtaatttga atggagctta tcttttcaat
     7801 gatgtagaac ccagtgagtt gaaaaaaatt gccagtgaat tagacacgac agaaacaacc
     7861 ttgaaaaatt caacgaatga ctattggtgg aaaaaattga ccgtctacta tatgattgac
     7921 gatgcagggg ttggtgtgaa aattttgaca tcaagtaaag atgccaataa cttgttagag
     7981 aaaaaaattg atgggttgat tgagaaattt aaacatgcct atgcaaatgt gaaaattgaa
     8041 aaactggaaa ccatcaactc taaagaattg aacgcaaatg gtgaaacagc gcttggctta
     8101 aatgtgaaaa atctgattct tcgtttagtt gttattggag tggtttgtgt gattttggtt
     8161 gtgatgggaa atgtgttagt ttatctcttt aatccaacaa tcaatagagt aggtgatttt
     8221 tctcagtatc aaattgattt tgtaacagag attacaacaa ttgctaacct agcagatgtt
     8281 ttgtcataca aaaatactgg acaggaattg accatcgtta gctcaaataa agctatctta
     8341 gataaattga aacagagtca agaagcttta aaaggaatgc attttgtaga tttacaggat
     8401 gtatcatctc ttttggaaag agatacagtc cttcttgttg aagagtacgg agtgactcgt
     8461 tataagaaat ttgagcaaag tcttcaaatt ctcagaaact taaatcgttc tatcctcggt
     8521 gtagcaacct ttaaattata agtattctga tgtataaggt cttcaaagtc ggctcttagc
     8581 cgattttgaa ggctttttct atctgtctgc agaaattttc ttttggagtc tcctatagaa
     8641 agaccctagg gaaaatctat ccgtatagag gagccttctt ttgataaaat cgtaaaaatc
     8701 tagtataata gatagaactg aaagtatgag gttactagca atgaaaatga aacaaattag
     8761 tgatacaact ttaaaaatca cgatatcttt agaggatttg atggatcgtg gaatggagat
     8821 tgctgacttt ctcgttcctc aagaaaaaac agaagagttc ttttatgcta tcttggatga
     8881 gctagagatg cctgatagct ttctggatac aggcatgttg agcttccgtg tgactccaaa
     8941 acctgataag gtagatgtct ttgtgaccaa gtcaaagatt gaccaaaatc tagattttga
     9001 agacttatcg gatttgccag atatggaaga attggctcaa atgtctccag atgaatttat
     9061 caaaaccctg gaaaaaagca tcgcagacaa aaccaaggat gatatcgaag ccattcaatc
     9121 tcttgagcaa gttgaagtca aggaagaaga gcaagagcag gctgaacaag aagctgagag
     9181 taagaaagaa ccttacatct actacatcct ttcttttgct aagttggctg acttggtagc
     9241 ttttgccaag acagtgactt ttgaaatgga aacttctgaa ctctacaaaa tgaacgagcg
     9301 ctattatttg accattttag tggatattga aaatcatcca agcccatatc cagcttggct
     9361 gttggcccgt atgcgcgagt ttgcagacga tagtgatatc agtcgctcag tcttacaaga
     9421 gtatggtcaa gtcttgatga gtcacgatgc agtgctcaat ctgcaaaaaa tcggctaatc
     9481 ctttctggaa agctattttc agattttaag aagagtgaat cgtctgattc gttttttctt
     9541 tttatactga aatagtgatt tactataata ggaattttca caaaattctg ttataatggc
     9601 tatattagaa aatttcgagg agataaagat gacagttaaa attgctttac taggatttgg
     9661 taccgttgca agtggtgtgc ctttcctcct aaaggaaaat ggaggaaaaa tcaatcaatc
     9721 agcacattca gatatcaaag ttgctaaggt attggtcaag gatgaagatg aaaaaaatcg
     9781 cttgcttgca gcagggaatg actttaactt tgtaaccaat gtggatgata ttttatcaga
     9841 ccaggatatt actatcgtag tggaattgat ggggcgtatt gagcctgcta aaacctttat
     9901 cactcgtgcc ttggaagctg gaaaacacgt tgttactgct aacaaggacc ttttagctgt
     9961 ccatggcgca gaattgctag aaatcgctca agctaacaag gtagcacttt actacgaagc
    10021 agcagttgct ggtgggattc caattcttcg tactttagca aattccttgg cttctgataa
    10081 aattacgcgc gtgcttggag tagtcaacgg aacttccaac ttcatggtga ccaagatggt
    10141 ggaagaaggc tggtcttacg atgatgctct tgcggaagca caacgtctag gatttgcaga
    10201 aagcgatccg acgaatgacg tagatgggat tgatgcagcc tacaagatgg ttattttgag
    10261 ccaatttgcc tttggcatga agattgcctt tgatgatgta gcccacaagg gaatccgcaa
    10321 tatcacacca gaagacgtag ctgtagctca agagcttggt tacgtagtga aattggttgg
    10381 ttctattgag gaaacttctt caggtattgc tgcagaagtg actccaacct tcctacctaa
    10441 agcgcaccca cttgctagtg tgaatggcgt aatgaacgct gtctttgtag aatctatcgg
    10501 tattggtgag tctatgtact acggaccagg tgcgggtcaa aaaccaactg caacaagtgt
    10561 tgtagctgat attgtccgta tcgttcgtcg tttgaatgat ggtactattg gcaaagactt
    10621 caacgaatat agccgtgact tggtcttggc aaatcctgaa gatgtcaaag caaactacta
    10681 tttctcaatc ttggctctag actcaaaagg tcaggtcttg aagttggctg aaatcttcaa
    10741 tgctcaagat atttccttta agcaaatcct tcaagatggc aaagagggtg acaaggcgcg
    10801 tgtcgttatc atcacacaca agattaataa agcccagctt gaaaatgtct cagctgaatt
    10861 gaagaaggtt tcagaattcg acctcttgaa taccttcaag gtgctaggag aataagatga
    10921 agattattgt acctgcaacc agtgccaata tcgggccagg ttttgactcg gtcggtgtag
    10981 ctgtaaccaa gtatcttcaa attgaggtct gcgaagaacg agatgagtgg ctgattgaac
    11041 accagattgg caaatggatt ccacatgacg agcgtaatct cttgctcaaa atcgctttgc
    11101 aaattgtacc agacttgcaa ccaagacgct tgaaaatgac cagtgatgtc cctttggcgc
    11161 gcggtttggg ttcttccagc tcggttatcg ttgctgggat tgaactagcc aaccaactgg
    11221 gtcaactcaa cttatcagac catgaaaaat tgcagttagc gaccaagatt gaagggcatc
    11281 ctgacaatgt ggctccagcc atttatggta atctcgttat tgcaagttct gttgaagggc
    11341 aagtctctgc tatcgtagca gactttccag agtgtgattt tctagcttac attccaaact
    11401 atgaattacg tactcgcgac agccgtagtg tcttgcctaa aaaattgtct tataaggaag
    11461 ctgttgctgc aagttctatc gccaatgtag cggttgctgc cttgttggca ggagacatgg
    11521 tgaccgctgg gcaagcaatc gagggagacc tcttccatga gcgctatcgt caggacttgg
    11581 taagagaatt tgcgatgatt aagcaagtga ccaaagaaaa tggggcctat gcaacctacc
    11641 tttctggtgc tgggccgaca gttatggttc tggcttctca tgacaagatg ccaacaatta
    11701 aggcagaatt ggaaaagcaa cctttcaaag gaaaactgca tgacttgaga gttgataccc
    11761 aaggtgtccg tgtagaagca aaataaagaa tagaagatag gatggggaaa ctcttgacca
    11821 gaggggttca tatccttttt gtgaaaagaa gtttatactc aatgaaaatc aaagagcaaa
    11881 ctaggaagct agccgcaggc tgctcaaaac agtgttttga ggttgcagat agaactgacg
    11941 aagtcagtaa ccatacctac ggtaaggtga cgctgacgtg gtttgaagag attttcgaag
    12001 agtattagtt aaaaacgtga taaaggagaa ataaagatgg cagaaattta tctagcaggt
    12061 ggttgttttt ggggcctaga ggaatatttt tcacgcattt ctggagtgct agaaaccagt
    12121 gttggctacg ctaatggtca agtcgaaacg accaattacc agttgctcaa ggaaacagac
    12181 catgcagaaa cggtccaagt gatttacgat gagaaggaag tgtcactcag agagatttta
    12241 ctttattatt tccgagttat cgatcctcta tctatcaatc aacaagggaa tgaccgtggt
    12301 cgccaatatc gaactgggat ttattatcag gatgaagcag atttgccagc tatctacaca
    12361 gtggtgcagg agcaggaacg catgctgggt cgaaagattg cagtagaagt ggagcaatta
    12421 cgccactaca ttctggctga agactaccac caagactatc tcaggaagaa tccttcaggt
    12481 tactgtcata tcgatgtgac cgatgctgat aagccattga ttgatgcagc aaactatgaa
    12541 aagcctagtc aagaggtgtt gaaggccagt ctatctgaag agtcttatcg tgtcacacaa
    12601 gaagctgcta cagaggctcc atttaccaat gcctatgacc aaacctttga agaggggatt
    12661 tatgtagata ttacgacagg tgagccactc ttttttgcca aggataagtt tgcttcaggt
    12721 tgtggttggc caagttttag ccgtccgatt tccaaagagt tgattcatta ttacaaggat
    12781 ctgagccatg gaatggagcg aattgaagtt cgttctcgtt caggcagtgc tcacttgggt
    12841 catgttttca cagatggacc gcgggagtta ggcggcctcc gttactgtat caattctgct
    12901 tctttacgct ttgtggccaa ggatgagatg gaaaaagcag gatatggcta tctattgcct
    12961 tacttaaaca aataaaacag agagtggggc ttcccacttt cttcatttct agaatatgaa
    13021 tagaagggat ttatgaaaca cctattatct tacttcaaac cctacatcaa ggaatcaatt
    13081 ttagccccct tgttcaagct gttagaagct gtttttgagc tcttggttcc catggtgatt
    13141 gctgggattg ttgaccaatc tttacctcag ggagatcaag gtcatctctg gatgcagatt
    13201 ggcctgctcc ttatctttgc agtaattggc gttttagtgg ccttgatagc tcaattttac
    13261 tcagcaaagg cagcagtagg ttctgctaag gaattgacaa acgatcttta tcgtcatatt
    13321 ctttccttgc ccaaggacag cagagaccgt ctgacaactt ctagtttggt cactcgcttg
    13381 acttcggata cctaccagat tcagactggt atcaatcaat tcctgcgtct ctttttacga
    13441 gcgcccatta tcgtttttgg tgccattttt atggcttatc gaatctcaac tgagttgact
    13501 ttctggttct tagtcttggt tgccattttg accattgtca ttgtagggtt atctcgattg
    13561 gtcaatcctt tctacagtag tctcagaaag aaaacggacc aactggttca ggaaacgcgc
    13621 cagcaattgc aagggatgcg ggttattcgt gcttttggtc aagaaaaacg agagttacag
    13681 atttttcaaa cccttaacca agtttatgct agattacaag aaaagacagg tttctggtct
    13741 agtttattaa cacctctgac ctatctgatt gtcaatggaa ctcttctcgt tattatctgg
    13801 caaggctata tttcaattca aggaggagtg ctcagtcaag gtgctctcat tgctcttatc
    13861 aattacctct tacagatttt ggtggaattg gtcaagctag ccatgttgat caattccctc
    13921 aaccagtcct atatctcagt caagcgaatc gaggaagtct ttgttgaggc tccagaggat
    13981 atccattcag agttagaaca aaagcaagct accagagata aggttttaca agtccaagaa
    14041 ttgaccttta cctatcctga tgcggcccag ccttctctga gatacatttc ctttgatatg
    14101 actcaaggac aaattctagg tatcatcggg ggaactggtt ctggtaaatc aagcttggtg
    14161 caactcttac ttggacttta tccagtagac aaggggaaca ttgaccttta tcaaaatgga
    14221 cgtagtcctc ttaatttgga gcagtggcgg tcttggattg cctatgtacc tcaaaaggtc
    14281 gaactcttta aaggaaccat tcgttccaac ttgactctag gtttcaatca agaagtatct
    14341 gaccaggaac tctggcaggc cttggagatt gcgcaagcta aggattttgt cagtgaaaag
    14401 gaaggactct tggattctct agttgaggca ggggggcgaa atttctcagg tggacaaaaa
    14461 caaagattgt ctatcgcccg agcagtcttg cgccaggctc cgtttctcat cctagatgat
    14521 gcaacctcgg cactggatac cattacagag tccaagctct tgaaagctat tagagaaaat
    14581 tttccaaaca cgagcttaat tttgatctct caacgaacct caactttaca gatggcggac
    14641 cagattctcc tcttggaaaa aggtgagttg ctagctgttg gcaagcacga tgacttgatg
    14701 aaatccagcc aagtctattg tgaaatcaat gcatcccaac atggaaagga ggactagaat
    14761 gaaacgacaa actgtaaacc agacgctcaa acgtttagcc gtagatttag caagccatcc
    14821 tttcctcctt ttcctagcct ttctaggaac tattgcccaa gttggcttat caatttacct
    14881 acctattctg attgggcagg tcattgacca agtcctagtg gctggttcat caccagtttt
    14941 ttggcagatt tttctccaga tgctcttggt ggtaatagga aatactctgg tacaatgggc
    15001 caatcctctc ctctataatc gtctaatctt ctcttatacc agagatttac gggagcgaat
    15061 catccataag ctccatcgtt taccgattgc ctttgtagat aggcaaggta gtggagagat
    15121 ggttagtcgt gtaaccacgg acatcgaaca gttggcagct ggcttgacca tgatttttaa
    15181 ccaatttttc attggtgttt tgatgatttt ggtcagtatt ctagccatgc tccaaattca
    15241 tctcctcatg actctcttag tcttgctgtt gacgccactg tccatggtga tttcacgctt
    15301 tattgccaag aaatcctatc atctcttcca gaagcaaaca gagacgaggg gaattcagac
    15361 tcagttgatt gaagaatcgc ttagtcagca gactataatc cagtccttca atgctcaaac
    15421 agaatttatc caaagattgc gtgaggctca tgacaactac tcaggctatt ctcagtcagc
    15481 catcttttat ttttcaacgg tcaatccttc gactcgcttt gtaaatgcac tcatttatgc
    15541 ccttttagct ggagtaggag cttatcgtat catgatgggt tcagccttga ccgtcggtcg
    15601 tttagtgact tttttgaact atgttcagca atacaccaag ccctttaacg atatttcttc
    15661 agtgctagct gagttgcaaa gtgctctggc ttgcgtagag cgtatctatg gagtcttaga
    15721 tagccctgaa gtggctgaaa caggtaagga agtcttgacg accagtgacc aagttaaggg
    15781 agctatttcc tttaaacatg tctcttttgg ctaccatcct gaaaaaattt tgattaagga
    15841 cttgtctatc gatattccag ctggtagtaa ggtagccatc gttggtccga caggtgctgg
    15901 aaaatcaact cttatcaatc tccttatgcg tttttatccc attagctcgg gagatatctt
    15961 gctggatggg caatccattt atgattatac acgagtatca ttgagacagc agtttggtat
    16021 ggtgcttcaa gaaacctggc tcacacaagg gaccattcat gataatattg cctttggcaa
    16081 tcctgaagcc agtcgagagc aagtaattgc tgctgccaaa gcagctaatg cagacttttt
    16141 catccaacag ttgccacagg gatacgatac caagttggaa aatgctggag aatctctctc
    16201 tgtcggccaa gctcagctct tgaccatagc ccgagtcttt ctggctattc caaagattct
    16261 tatcttagac gaggcaactt cttccattga tacacggaca gaagtgctgg tacaggatgc
    16321 ctttgcaaaa ctcatgaagg gccgcacaag tttcatcatt gctcaccgtt tgtcaaccat
    16381 tcaggatgcg gatttaattc ttgtcttagt agatggtgat attgttgaat atggtaacca
    16441 tcaagaactc atggatagaa agggtaagta ttaccaaatg caaaaagctg cggcttttag
    16501 ttctgaataa gccattctct tttgaaagtt tatggacgaa aaaagttgcc ttcgagtgac
    16561 ttttttgtta caatagctag aaaaattgtt cactgtaata ctcaatgaaa atcaaagagc
    16621 aaactaggaa gctagccgta ggttgctcaa agcacagctt tgaggttgta gataagactg
    16681 acgaagtcag ttcaaaacac tgttttgagg ttgc
//
LOCUS       DBDESG010000055        15332 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.055, whole genome shotgun sequence.
ACCESSION   DBDESG010000055 DBDESG010000000
VERSION     DBDESG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.055"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..2333)
                     /locus_tag="ACKB38_001726"
     CDS             complement(<1..2333)
                     /locus_tag="ACKB38_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGL7980456.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEQNFLRKSMLRALPSALMVVFSVLFVKMF
                     GASQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQAK"
     gene            2466..3215
                     /locus_tag="ACKB38_001727"
     CDS             2466..3215
                     /locus_tag="ACKB38_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGL7980457.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(3507..4121)
                     /locus_tag="ACKB38_001728"
     CDS             complement(3507..4121)
                     /locus_tag="ACKB38_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGL7980458.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(4937..5206)
                     /gene="rpsO"
                     /locus_tag="ACKB38_001729"
     CDS             complement(4937..5206)
                     /gene="rpsO"
                     /locus_tag="ACKB38_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGL7980459.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(5347..5976)
                     /locus_tag="ACKB38_001730"
     CDS             complement(5347..5976)
                     /locus_tag="ACKB38_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGL7980460.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            6213..6413
                     /locus_tag="ACKB38_001731"
     CDS             6213..6413
                     /locus_tag="ACKB38_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980461.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            6844..7167
                     /locus_tag="ACKB38_001732"
     CDS             6844..7167
                     /locus_tag="ACKB38_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGL7980462.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            7225..9168
                     /gene="thrS"
                     /locus_tag="ACKB38_001733"
     CDS             7225..9168
                     /gene="thrS"
                     /locus_tag="ACKB38_001733"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGL7980463.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELVSGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(9217..10191)
                     /locus_tag="ACKB38_001734"
     CDS             complement(9217..10191)
                     /locus_tag="ACKB38_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGL7980464.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(10184..10861)
                     /locus_tag="ACKB38_001735"
     CDS             complement(10184..10861)
                     /locus_tag="ACKB38_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7980465.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            11017..12090
                     /locus_tag="ACKB38_001736"
     CDS             11017..12090
                     /locus_tag="ACKB38_001736"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGL7980466.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(12181..12483)
                     /locus_tag="ACKB38_001737"
     CDS             complement(12181..12483)
                     /locus_tag="ACKB38_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980467.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYIFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(12551..12988)
                     /locus_tag="ACKB38_001738"
     CDS             complement(12551..12988)
                     /locus_tag="ACKB38_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGL7980468.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(13064..13906)
                     /locus_tag="ACKB38_001739"
     CDS             complement(13064..13906)
                     /locus_tag="ACKB38_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGL7980469.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            14018..14668
                     /locus_tag="ACKB38_001740"
     CDS             14018..14668
                     /locus_tag="ACKB38_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGL7980470.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            complement(14740..>15332)
                     /locus_tag="ACKB38_001741"
     CDS             complement(14740..>15332)
                     /locus_tag="ACKB38_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980471.1"
                     /translation="LFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIK
                     RYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQ
                     NKKPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKL
                     IKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         4662 a         3445 c         2895 g         4330 t
ORIGIN      
        1 tttttcgctt ggtaacgact ggtcaggatg aaaatcacgg taaagaccaa catcatgaca
       61 ccataaacag gcaacgtttg ttctgttaag gttgaaattt caagcagttt ttgaattctt
      121 gggaagagag ctgtggctag gaaacctcct actgaccaaa caatcaagag gacacgccat
      181 agggtaaatg gcatgcaggc tctaaatacg gataagaaac caattgaccc caagagataa
      241 tagagtagag ttgagatttc taactcagac caaccttgac tcgcgccaaa cattttcaca
      301 aacaggacgc tgaagacgac catgagagcg cttggtaggg cacgaagcat ggattttctg
      361 aggaaattct gctcaacagg tttgatattt cgctcaaaag tcagaacgaa tggtgggaaa
      421 ccttccacaa actggtcaat catggtaatc tggatcggaa tgaaggggaa aatcaaaatc
      481 cactctgacc gacctagtaa agcactggca atacagatga ctgctaacag gaaggaatag
      541 atggtcttta tcaagaaaat cggggcgatg tgggcaatgt tattgaccac gcgacgaccc
      601 tcgaagagaa tctcaggaac atcattaaag tctgagttca agagaaccag attggcaatc
      661 tgacgggttg ctggatcccc ctccgccatc acgatagaac aatccgcctc acgaagggcc
      721 aagatatcat taaccccgtc ccctgtcata gccgttgtat gtcccgcttt tttcaacgtt
      781 tggatgatga gtttcttttg atgaggggaa acacgtccga aaatagctgt ctcctccgcc
      841 atggccatca attcctcatc ggtgattttt gagcaatcta catagctgtg atagtccgca
      901 aaaccagcct tctgggcaat gctggacacc gtaactggat tgtcaccaga gataatcttg
      961 agtcccacct cctgagaacg gagatagtcc agcgtctctg ctgctccctc tcgaatgggg
     1021 tccaagattt ccagcaaggc tagagcctga atatcagatg gtttctgtgg tttgtgatgg
     1081 tctaatttct cctgactgag agctaagacc aagacacgtg atcctctctc caaggcctcc
     1141 ctagcttctg ggacttcaga atcaagcaac atctcaggtg cccctaagaa aactgtcccc
     1201 aagccttcta actccatagc cccccacttg cggtcgctcg agaagggaag attggaaatc
     1261 ataggataag caacatctcc cacaaaacgc tggcgaatgg cttgggcagt tggattctta
     1321 tcctcactat gggccatgta gctagtcaag atgctggcaa tagcctcttc accatacgtt
     1381 tccgtcaacg gaagaacagc ctccacctgc atctttcctt gggtgatggt acccgtcttg
     1441 tccagacaga gcatatccac gcgcgccaag gtctcaacag agtacatctc ctgcaccaag
     1501 acctttttca agcccaactt aatcactgca gtcaagagcg aagtaatggt caaaagggca
     1561 attcccttag gcaacattcc caaaagagct gtcgacgagt ttacaacgga tgacttgaga
     1621 ggcaggcctt ttaaaagcaa ggcttccagc aagagagcca gaccaaaggg aatgataatc
     1681 ttcccagtaa aaccagccaa cttgtccagc gatttcatga tacgggagtt gatgggttta
     1741 acggtcttag cctcaagcat gagtttggca gcatagttgt ctgcaccgac atggtgaact
     1801 tgagataaaa ctgacccact ggctaggaaa cttcctgaca aaagtaagcc gtcaacttcc
     1861 ttttgcacca aatcactttc tcccgttaac atggcttcat tgacttccgc aaagccttcc
     1921 aaaaccaagg catcactagg aatctgctct cctgcagaca aacgaatgac atctcctagc
     1981 actaattctt caggattaag agcaacttcc tgaccatcac ggatggtttt gaccttttcc
     2041 ttggtcatga gattgagctt gtccaccatg tgtttggctc gtagctcggt cacaatccca
     2101 gaaaaagcgt taaagcagat aacagcaaag aagaccagat tgctccaagc ctgcacaaag
     2161 gcaagagcca aagcaatggc aaagttcaaa gcgttaaaaa gggtaaagac atttcgttta
     2221 acgatttgcc aagtgctggt actggctgat gcggtaaagt cattgaccaa accctcagcc
     2281 tgtctttcct tgacttctct ttgggttaat cccataatct tatttttatc cataaattct
     2341 attatatcat tttttttaac agaattctaa tttcatccta aatatgtatc tagtcaaggg
     2401 aaaagtttgc cagccctcct ttgataaggt ataataataa caagaaaaca atcgaaggag
     2461 atcgcatgtt ttatacttat ttgcgtggat tagttgtatt gctcttatgg tccatcaatg
     2521 gcaatgctca ctatcataat actgataaaa ttcctaatca agatgaaaat tatattttag
     2581 ttgcgcctca ccgtacctgg tgggatcctg tttatatggc ctttgcgacc aagccaaaac
     2641 agttcatctt tatggcaaaa aaagaactct ttaccaaccg tatctttggt tggtggattc
     2701 gtatgtgtgg cgcctttccc atcgaccgtg aaaatcccag cgcctcagcc atcaaatatc
     2761 ctatcaacgt tctcaaaaaa agtgaccgct ctctcatcat gtttccaagt ggtagccgcc
     2821 actcaaacga tgtcaagggg ggcgcagcac tgattgccaa aatggccaag gtccgtatca
     2881 tgccggttac ctacaccggt cccatgactt tgaagggctt gattagccgt gaacgtgtcg
     2941 atatgaactt tggaaatcca atcgatatct cagatatcaa gaaaatgaat gatgaaggca
     3001 ttgaaacagt cgccaatcgt attcaaacag aattccaacg tctggacgaa gaaacgaaac
     3061 aatggcacaa tgataaaaaa ccaaatccac tctggtggtt tatccgcatc cctgccctca
     3121 tccttgctat tatcctcgct atcctaacca tcatctttag ctttatcgca agcttcatct
     3181 ggaacccaga taagaaaaga gaagaacttg catagaagaa atgaaccttg gccaaacagc
     3241 taaggttttc atttatatag tagattggaa ctagaatagt acacctctac ttctaaaaca
     3301 tttttagaaa tcgatttgat tgtcctgatc gatttgtcct attcttattt cattttacta
     3361 tatttataag aatctcctaa tcaaatcaaa agccactcta caatagagta actttaagat
     3421 tgtccattta tactcaatga aaaagccctc tgaaaatcag agagctgatt tgagctcggg
     3481 ctaaaatcct agtgaaacaa aaaaatctac acgatcagaa aagtctctat cgtgccattt
     3541 tcatacatga tgtatagtcc aagtagaatg aatactaagg gcacaatgat tcgctcgtat
     3601 ttttcaattg tctcgaatat taacggaata gaggataaca cccgactaat ctcgcaaaag
     3661 ataattatgc cgattacaaa cacaagcaag gccacgaggg tctgtgacca atctaacgaa
     3721 gcaaaataag gtatatagat acctaaatta tctccgccag acgcaattgt cagcaatgta
     3781 actgtccaaa acagttgatt tgccttgctt tgttctaatc tttcaataat ttcttcctct
     3841 tcttcttccg catcttctcc aacaattgca aagcgaatcc ctaaatagat agggattaaa
     3901 ccaagcaatc caaccatcca ttcttcaggc acgaaattaa cgacataagc agcaactaaa
     3961 ctcgccccta caagtaagcc tgtgcctaga tattgccccg cataaatatg ccatttctgt
     4021 ttattctgtg atagctgtgc aaataaaata attaaaataa ttaaataatc gatactggtg
     4081 gaaatataaa caccaatagc agatatgatt gtctgtccca taaaaaaaac ctcctgtatt
     4141 agtcagtaat aaatcagcag attttaggag agcacagaca cattaattta gctatcgcta
     4201 gtgggtaata tcgaacgtag gaaagcattt tactcctcct caaaacgtag ttacaaagta
     4261 atttctaact ggaattcagt gactacttcc atgtaaagta taacatacta tactttactt
     4321 tgtaaagagt gggctcttct agggggcttg catacagcta ctcggcttga aaagccgagt
     4381 aactgcgcac ctttatggtg taattagctg ataccgtttg aggtttttgt agtctccaaa
     4441 attgttaccg ataaaacctc aataaaaaag ccctctgaag tcagagagct gatttgagcc
     4501 cgggctaaaa tcctagtgaa aaagatgaaa ctccttgtgc tcatcgaaca ctgtgtcatt
     4561 tccctatttt catacggatt tttgacgccc ttagcatcat gattcttgct agataaatcg
     4621 tttatagact aggcgacgag gcgaagatcg tacgaaactt tcagtgaaac aaaaaaaatc
     4681 tccccgaagg gagaaaagct ggtttatttt accttacagt gctaggaacc gtaaccaaag
     4741 attatacttg agtatatcga ggtaaggtga caacgcaaaa aagctaccca atttagagta
     4801 gctttattat caagatatgc tcaattgaac acggcctaag cacttggcaa aaaagataaa
     4861 atctcctaga aactgaagtt tcttcgtcaa ttttcctatt tttgctttgt gcttttgacg
     4921 gcctttgtat cttgaattag cgacgaagtc ctagagagtt gatcaactca cggtaacggt
     4981 taacgtcgtt tttacgcaag tatgcaagca agttacgacg gcgaccgatt tttttcatca
     5041 atccacggta agtagcgtgg tcttttttgt gttgtttgat gtgttcgtta aggtggttga
     5101 tttcccaagt aaggacagca acttgaacct ctactgaacc tgtatcacct tcgtgacgtg
     5161 catattgtgc gatgatttca ttttttttct cttttgagat tgccatgatg tttctccttt
     5221 ttatttggct tcatccgagt gacaggttgg catgcctgta accaagaaga agttatttgt
     5281 ctttacgaca atccctattt taccaagatt taaattttct gtcaaacatt aatactctca
     5341 tagttattat ttttcaagta aagagatacg ttcttttata tgatgacctt tttcaagttc
     5401 atctaccaat ccaatagcat aatctgcata actaatctgg cttattcctt tttcattagt
     5461 agtaaaaatt tctcctgcta gaatgtagtt tcctgttttt tcaccatcgg gaataaaatc
     5521 tacagcagga ctgacaaaag tccagttgag attatttttt gtacgaagaa gatccaattc
     5581 atccgcttga gcttttgcta gtggcttaaa ttcttcagga aaatcaggcg tatctaaaag
     5641 tcgtgtcgtt tttgtttcat ctatataaag acttcctgct ccccctacaa ctagaaaacg
     5701 tgtctgtgtt ccagcaagaa gctgattaaa tagttcgatt gatttgctgt ggagcggtag
     5761 cgtatctggt gtataagcac caaacgctga aataacagca tcaaatccag taagatcatc
     5821 ttttgtcaac tcaaataaat cttttttaat aatagactca gcttgacttt tgttttcaga
     5881 acgaacaata gccgttactt catgtcctcg tttgactgct tcttcaacaa ttgctttccc
     5941 cgcttgtcca tttgctgcaa taactgctag tttcattttt tatacctctc ttgttgtaat
     6001 tattttagtt acagaaattg tgacactctt aataatcaat gtcaatagtc ttgcttaatt
     6061 attatcaaaa tatttctacc aagaaaacta accatgattc tagtgaaaaa aaatcttctt
     6121 tgtcaacaaa tttactttct tgttttaaac atgctataat aatcatagca agagatctaa
     6181 gttgtctgtt tttttaaaac gaggtgatta tcatgcgtag attctattcc catctcccct
     6241 actatctggt catattattc ttttattggc cactttatga gttgttctta ctagttgttt
     6301 ctgaccccct tacactcaag ggactctata taaacaatct tctcttcttt acacctctgg
     6361 taatcttgat tgtatcgtta ctctatagct accgtttccg tttctcactt tgatggttag
     6421 ttggtaacgg actgctcttt tactttacta tcataacctt tggtgagttt atactaattt
     6481 acttgctaat ctatgaaaca gttgctctgg tcggcatgga ttctggtatt agcatcaagc
     6541 atattctaca aaaaatgaaa aacaaaaaac tttcacaaaa tccttgaaaa atctcacaat
     6601 catgctataa taatccatag agacaagtca cttagtccct ttctactaga gagtgcgtgg
     6661 ttgctggaaa cgcataggaa gtctaaactg atactactct tgagtttttt atgaaaacat
     6721 aaaacggtgg ccacgttaga gccgatcaga ggtgtccctc tcttttgagg tacataaatg
     6781 aaggtggaac cacgttgcga cgtcctttcg aggatgtcgc atttttttat taggatacta
     6841 attatggagt tgcaagaatt agtggagcgc agttgggcaa tccgacaagc ttatcacgaa
     6901 ctggaagtta agcatcatga ttccaagtgg acggtagaag aagacctctt ggctttatct
     6961 aatgatattg gaaatttcca acgactggtg atgacaaagc aaggacgcta ctatgatgaa
     7021 acaccctaca cactggaaca aaaactttca gaaaatatct ggtggctatt agaactttct
     7081 caacgtttgg atatagacat tctgacggaa atggaaaact tcctctctga taaagaaaag
     7141 caattgaacg ttaggacttg gaagtagtct gctgataaaa aatcaatgct tagaaactat
     7201 gaaataataa aaaaggagaa catcatgatt aacattactt tcccagatgg cgctgttcgt
     7261 gaattcgaat ctggcgtaac aacttttgaa attgcccaat ctatcagcaa ttccctagct
     7321 aaaaaagcct tggctggtaa attcaacggc aaactcatcg acactactcg cgctatcact
     7381 gaagatggaa gcatcgaaat tgtgacacct gatcacgaag atgcccttcc aatcttgcgt
     7441 cactcagcag ctcacttgtt cgcccaagca gctcgtcgtc ttttcccaga cattcacttg
     7501 ggagttggtc cagccatcga agatggtttc tactacgata ctgacaacac agctggtcaa
     7561 atctctaacg aagaccttcc tcgtatcgaa gaagaaatgc aaaaaatcgt caaagaaaac
     7621 ttcccatcta ttcgtgaaga agtgactaaa gacgaggcac gtgaaatctt caaaaatgac
     7681 ccttacaagt tggaattgat tgaagaacac tcagaagacg aaggcggttt gactatctat
     7741 cgtcagggtg aatatgtaga cctctgccgt ggacctcacg ttccatcaac aggtcgtatc
     7801 caaatcttcc accttctcca tgtagctggt gcgtactggc gtggaaacag cgacaacgct
     7861 atgatgcaac gtatctacgg tacagcttgg tttgacaaga aagacttgaa aaactacctt
     7921 caaatgcgtg aagaagctaa ggaacgtgac caccgtaaac ttggtaaaga gcttgacctc
     7981 tttatgattt cacaagaagt gggacaaggt ttgccattct ggttgccaaa tggtgcgact
     8041 atccgtcgtg aattggaacg ctacatcgta aacaaagagt tggtttctgg ctaccaacac
     8101 gtctacactc caccacttgc ttctgttgag ctttacaaga cttctggtca ctgggatcat
     8161 taccaagaag acatgttccc aaccatggac atgggtgacg gggaagaatt tgtccttcgt
     8221 ccaatgaact gtccgcacca catccaagtt ttcaaacacc atgttcactc ttaccgtgaa
     8281 ttgccaatcc gtatcgctga aatcggtatg atgcaccgtt acgaaaaatc tggtgccctc
     8341 actggccttc aacgtgtacg tgaaatgtca ctcaacgacg gtcacctatt cgttactcca
     8401 gaacaaatcc aagaagaatt ccaacgtgcc cttcagttga ttatcgatgt ttatgaagac
     8461 ttcaacttga ctgactaccg cttccgcctc tctcttcgtg accctcaaga tactcataag
     8521 tactttgata acgatgagat gtgggaaaat gcccaaacca tgcttcgtgc agctcttgat
     8581 gaaatgggcg tggactactt tgaagccgaa ggtgaagcag ccttctacgg accaaaattg
     8641 gatatccaga ttaaaactgc ccttggaaaa gaagaaaccc tttctactat ccaacttgat
     8701 ttcttgttgc cagaacgctt cgacctcaaa tacatcggag ctgatggcga agatcaccgt
     8761 ccagtcatga tccaccgtgg ggttatctca actatggaac gcttcacagc tatcttgatt
     8821 gagaactaca agggggcctt cccaacatgg ctggcaccac accaagtaac cctcatccca
     8881 gtatctaacg aaaaacacgt ggactacgct tgggaagtgg ccaagaaact ccgtgaccgc
     8941 ggtgtccgtg cagacgtaga tgagcgcaat gaaaaaatgc agttcaagat ccgtgcttca
     9001 caaaccagca agattcctta ccaattaatt gttggagaca aagaaatgga agacgaaaca
     9061 gtcaacgttc gtcgctacgg ccaaaaagaa acacaaactg tctcagttga taattttgtt
     9121 caagctatcc tagctgatat cgccaacaaa tcacgcgttg agaaataaga gtctagcata
     9181 aaagcctcca atctggaggc tttttctcat ctatttttac tcaaggacta agttcacttg
     9241 agcaaactga atccgcactg tcgttccttt tccgacctca gactcgatac gaatctggtg
     9301 ccccagttct tcagaaattt tcttagatag ataaaggcca agtccagagg actgctgggt
     9361 caaacggcca ttgtatcctg aaaagccacg ttcaaatact cggaggacat cactgttttt
     9421 tatcccgatt cccgtatctt tgatacaaag ctcttggtca tccatataaa tctccagacc
     9481 accttccttg gtgtacttga gactgtttga gatgatttgc tcaataacca ctagcagcca
     9541 ctttttatcc gtcacgattt ctttatcaag gtcatgtaga ttgacattta agcctttttg
     9601 aataaagaaa agagcatatt tacgaattat ttccttgacc aagtcctcaa tttgaacctg
     9661 ctttaagacc aaatcatcat ggaaactttc taaacgcagg tactgtaaaa ctaggttggt
     9721 ataggagtcg attttgaaaa tttcctgttc tagctgctgc ttcagttggc ggtcgaccac
     9781 ttctgcaact aagagttgac tggctgcaat gggggtcttt atctgatgga cccacaaggt
     9841 atagtaatcc agcaaatccg tcagttttct ttctgctttt gacctctgct gatagagttc
     9901 catctcacgc gcttctaatt tttctgctaa agctatttcc aaaggagact tggcttccct
     9961 ctctccatag agaagttcct ggcgatagac ctgcgtttcc accaatatgt cccaagtgaa
    10021 aaataatatg gttacaaagc aacacaagaa gaaaaagtag aggaagtaaa ttcctagact
    10081 ggcaaataaa aactgaaaga gtaagacaag aaatgccaaa gaaagcagat agataaaaag
    10141 acgactacgg gagcgcagat aggctagaaa aaattgtttc caatcaagca tgcttcaatc
    10201 cgtaccctat tcctttcttg gtctcgataa atcctaccaa tccctgctcc tccaactttt
    10261 tacgcaaacg agccacattg acagagaggg tattatcatc aatgaaaaag tcactgttcc
    10321 aaagttcccg catcaggtcg tcacgtgcta cgatgttgcc tgcatgctca aataacacgc
    10381 gtaaaatctg gaattcattc ttggtcaaat tcaagacttg cccttgataa tgtaaatcca
    10441 tggatttggt attgaggata acaccagcat attccagcaa actctcatca cgcccaaact
    10501 cataggaacg acgcaacaag ccctgaacct tagctaaaag aacctgctgg tcaaaaggct
    10561 tggtcacaaa gtcatccgcc cccatattga ttgccatgac aatatccata gcctggtctc
    10621 tcgaagaaag aaacatgata ggtaccttgg aaatcttgcg gatttcctga caccagtgat
    10681 aaccattaaa caagggcaaa ccaatatcca tgaggaccag atgaggttcc gactgaacaa
    10741 atagactcaa aacttccata aagtcttcta ccaggaccac ttcaaatccc cattcagaga
    10801 gcattttccc aatctgttga cgaatgacct gatcatcttc tattaataaa atcttgtgca
    10861 tgcgcttctc cttttccatt attataacag atttttccat gctagatggt ctgaaactga
    10921 atttgaaata gcctgttttt agccagtaca aacaggctat gctactagct aatttgaggg
    10981 aaatttgcta agataaataa aaagaaagga gctcttatgg ccaatatttt tgactatctg
    11041 aaagatgtcg catatgattc ttattacgac cttcccttga atgagttaga cattctaacc
    11101 ttaatagaaa tcacctacct ctcctttgat aatctggtct ccacacttcc tcaacgtctt
    11161 ttagatctag cacctcaggt tccaagagat cccaccatgc ttactagcaa aaatcgcctt
    11221 caattattag atgaattggc tcaacacaag cgcttcaaaa attgcaaact ctcccatttt
    11281 atcaacgaca tcgaccctga actgcaaaag caatttgcgg ctatgactta tcgtgtcagc
    11341 ctcgatacct atctgattgt ctttcgtggg acagatgaca gtatcattgg ctggaaggaa
    11401 gatttccacc tgacctatat gaaggaaatt cctgctcaaa agcacgccct tcgctattta
    11461 aagaactttt ttgcccatca tcctaagcaa aaggttattc tagctgggca ttccaaggga
    11521 ggaaatctcg ctatctatgc tgctagccaa attgagcaaa gtttgcaaaa tcagatcaca
    11581 gcagtttata catttgatgc acctggtctc catcaagaat tgacacagac tgcgggttat
    11641 caaaggataa tggatagaag caagatattc attccacaag gttccattat cggtatgatg
    11701 ctggaaattc ctgctcacca aatcatcgtt cagagtactg ccctgggtgg catcgcccag
    11761 cacgatacct ttagttggca gattgaggac aagcacttcg tccaactgga taagaccaac
    11821 agtgatagcc agcaagtaga cacaaccttt aaagaatggg tggccacagt ccctgacgaa
    11881 gaacttcagc tctacttcga cctcttcttt ggcactattc ttgatgctgg tattagctct
    11941 atcaatgact tggcttcctt aaaggcgctt gaatacattc atcatctctt tgtccaagct
    12001 caatccctca ctccagaaga aagagaaacc ttgggtcgcc ttacccagtt attgattgat
    12061 actcgttacc aggcatggaa aaatagataa tactcttgaa aattaaatgt atacaaaaca
    12121 aaagacctag aatacatact ttcatgtgca ttctaagtct ttttaaatag aatctaatag
    12181 tcaataaaaa tcaaagagca ttgagagata atggggcttg gaacgtccct ctcgcttcaa
    12241 caaaatgacc ccattataga ttaaaaagat gccacttaga aaaagcaaaa aaggaagtaa
    12301 gacaaaggca aatatataaa aagctaactg aacattctcg tatccatttt tataaaaaag
    12361 gtaggataga taaaaataac ttgaaatgag ggataataaa aataatactg gattccacaa
    12421 acttctatta tccttccaaa atgacactat aaaggctaat acaattccta taacgagata
    12481 catttcttac tcctttaata gctacatttt atcataatta tccaaagaaa aaagagggca
    12541 tttatccctc ttaatccttc atctgactct ctgcatcggc cacgactttt tctagactgg
    12601 tttgaccaag ttctgcctcc atagtcaact gaattctctc caatttttga tccaaaacat
    12661 catgaatatg agctcctaca gggcaatttg gattcggatt gtcatggaaa ctgaagagtt
    12721 gacctgtctt accaagacat tcgaccgcct gataaacatc taaaagacta atatccttaa
    12781 ggtccttgac aatctctgtt ccgcccgttc cacgcgctac tgaaatcagc tctgccttct
    12841 tcaactggga caagatcttt ctgataatga caggattgac cccgacacta gcagccagaa
    12901 aatcactggt caccttgctt tccttcccct cgagggcaat gattatcagc atatgagtcg
    12961 caatggtaaa tctacttgga atttgcatcc tcttctcctt tttacgaggc taccctgcct
    13021 ctactcttct ttttctatta ttataccctt tttagttgta atgtcaatcg ttaccacttt
    13081 tcaaccagtc gtctaactcc cgatcgcagc cctctttctg agccaattct ctcaaaaatt
    13141 cctgatgatg agtatggtgg atcccattga ccagactttc atagtaaacc tcaaaatagg
    13201 gaagtctcag gtctttagcc agctgcaatt cagctgctac atcgtagtct acccgtcgga
    13261 agtccatatc taccaggcct ttgtcatcaa actccaaaat catatactgg gcccgcaagt
    13321 ccttccgtag ctgagcgtcc aaaaagaaag gttggccaat cgaacccgga ttgacaatca
    13381 attgcccacc agtcccgtaa cgaagcaact gctggtgaat atgtccataa acagcaatat
    13441 cacagggagg atgagtcacc aagcggtcaa actcctcttg tttgccagta tgaatcaact
    13501 ctcgccccca gttcttatca ggcagatgat ggctaattcc caccgtcaaa tccccaaact
    13561 gacgatgaat ttgaagaggt tgattgtgga gcacttcaat ttcttctagg gaaatttcct
    13621 ctaaaacata ctggcactgg cgcaagagat agcgttgact ggggcgagta ctgtccaatt
    13681 ccttacggac accatgccaa agactgtctt cccagtttcc caaaactcta gccgtaatcg
    13741 gtagttgatc caacaagtcc aaaatccttc tacgccctgt ccctggcatg agaatatctc
    13801 ccaaaagcca gtattcatcc actcctatct gccgagcatc tgccaaaaca gcctccaagg
    13861 cggtggtatt tccatgaata tctgaaagaa gagctatttt cgtcatatcc atctcctcgt
    13921 tttttctctt gcaataagta taacataaaa agtcacagct agagaaatct agcttttttt
    13981 gatatactag ataaagatat tagacaagag gaaacgaatg accccaaaca aagaagacta
    14041 tctaaaatgt atttatgaaa ttggcataga cctgcataag attaccaaca aggaaattgc
    14101 ggctcgcatg caagtctctc cccctgccgt aactgaaatg atcaaacgaa tgaaaagtga
    14161 aaatctcatc ctaaaggaca aggaatgtgg ctatctactg actgacctcg gtctcaaact
    14221 ggtctctgag ctctatcgta agcaccgctt gattgaagtt tttctagttc atcatttaga
    14281 ctatacaagt gaccagattc acgaggaagc tgaggtcttg gaacacactg tctctgacct
    14341 gttcgtggaa agactagata aactgctagg tttccctaaa acctgccccc acgggggaac
    14401 tattcctgcc aagggagaac tactcgttga aatcaataac ctcccactag ctgatatcaa
    14461 ggaagctggc gcctaccgcc tgactcgggt gcacgatagt tttgacattc tccattatct
    14521 ggacaagcac tcacttcaca tcggtgacca gctccaagtc aagcagtttg atggcttcag
    14581 caataccttc actatcctca gtaacgacga ggatttacaa gtgaatatgg acattgcaaa
    14641 acaactctat gtcgagaaaa tcaactaatt tctcaagtcc cctaccaacc ctgaaagttt
    14701 tattttggct ctttgtcaac tgtagtgggt tgaagtcagc taagctcgag aaaggacaaa
    14761 ttttgtcctt tcttttttga tattcagagc gataaaaatc cgttttttga agttttcaaa
    14821 gttccgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg tcgcttccag
    14881 tttggcatta gaatagtgta gttgaagggc gttgacaatc ttttctttat ctttgaggaa
    14941 ggttttaaag acagtctgaa aaataggatg aacctgcttt agattgtcct caatgagtcc
    15001 gaaaaatttc tccggtttct tattctgaaa gtgaaacagc aagagttgat agagctgata
    15061 gtggtgtttc aagtcttgtg aatagctcaa aagcttgtct aaaatctctt tattggttaa
    15121 gtgcatacga aaagtaggac gataaaatcg cttatcactc agtttacggc tatcctgttg
    15181 tatgagcttc cagtagcgct tgatagcctt gtattcatgg gattttcgat gaaactgatt
    15241 catgatttga acacgcacac gactcatagc acggctaaga tgttgtacaa tgtgaaagcg
    15301 atccaacacg attttagcac acgggaaaag tt
//
LOCUS       DBDESG010000056        14635 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.056, whole genome shotgun sequence.
ACCESSION   DBDESG010000056 DBDESG010000000
VERSION     DBDESG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.056"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(63..1685)
                     /gene="groL"
                     /locus_tag="ACKB38_001742"
     CDS             complement(63..1685)
                     /gene="groL"
                     /locus_tag="ACKB38_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGL7980472.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1701..1985)
                     /gene="groES"
                     /locus_tag="ACKB38_001743"
     CDS             complement(1701..1985)
                     /gene="groES"
                     /locus_tag="ACKB38_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGL7980473.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2141..2536)
                     /locus_tag="ACKB38_001744"
     CDS             complement(2141..2536)
                     /locus_tag="ACKB38_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGL7980474.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2614..3375)
                     /locus_tag="ACKB38_001745"
     CDS             complement(2614..3375)
                     /locus_tag="ACKB38_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGL7980475.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3417..4043)
                     /gene="ytpR"
                     /locus_tag="ACKB38_001746"
     CDS             complement(3417..4043)
                     /gene="ytpR"
                     /locus_tag="ACKB38_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGL7980476.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4059..4376)
                     /locus_tag="ACKB38_001747"
     CDS             complement(4059..4376)
                     /locus_tag="ACKB38_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGL7980477.1"
                     /translation="MIQPASLEELASLVEKAGKKVFIFVADWCSDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4373..4672)
                     /locus_tag="ACKB38_001748"
     CDS             complement(4373..4672)
                     /locus_tag="ACKB38_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGL7980478.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            4766..4897
                     /locus_tag="ACKB38_001749"
     CDS             4766..4897
                     /locus_tag="ACKB38_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980479.1"
                     /translation="MKIKDQTRKLAAGCSKHCFEVVDRTDEVSSKHCFEVVDEVDVV"
     gene            complement(4922..5002)
                     /locus_tag="ACKB38_001750"
     CDS             complement(4922..5002)
                     /locus_tag="ACKB38_001750"
                     /inference="COORDINATES: protein motif:HMM:NF039231.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980480.1"
                     /translation="MASSGKMLRNTYTPDSYYVGNLGAWQ"
     gene            complement(5009..5200)
                     /locus_tag="ACKB38_001751"
     CDS             complement(5009..5200)
                     /locus_tag="ACKB38_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HGL7980481.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5361..6035)
                     /locus_tag="ACKB38_001752"
     CDS             complement(5361..6035)
                     /locus_tag="ACKB38_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980482.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6041..6487)
                     /locus_tag="ACKB38_001753"
     CDS             complement(6041..6487)
                     /locus_tag="ACKB38_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGL7980483.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6601..7104)
                     /locus_tag="ACKB38_001754"
     CDS             complement(6601..7104)
                     /locus_tag="ACKB38_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGL7980484.1"
                     /translation="MKNYQEWYRNISSRLTSCPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7101..7463)
                     /locus_tag="ACKB38_001755"
     CDS             complement(7101..7463)
                     /locus_tag="ACKB38_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HGL7980485.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7529..8560)
                     /locus_tag="ACKB38_001756"
     CDS             complement(7529..8560)
                     /locus_tag="ACKB38_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGL7980486.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVRQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            complement(8567..9388)
                     /locus_tag="ACKB38_001757"
     CDS             complement(8567..9388)
                     /locus_tag="ACKB38_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980487.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQETNLDELWQSA
                     SMMMGMSFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGSIMAIWAIGKILGHSEYWLWAVLVAVIINVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            complement(9405..9876)
                     /locus_tag="ACKB38_001758"
                     /pseudo
     CDS             complement(9405..9876)
                     /locus_tag="ACKB38_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(10000..10716)
                     /locus_tag="ACKB38_001759"
     CDS             complement(10000..10716)
                     /locus_tag="ACKB38_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGL7980488.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11597..13012)
                     /gene="ply"
                     /locus_tag="ACKB38_001760"
     CDS             complement(11597..13012)
                     /gene="ply"
                     /locus_tag="ACKB38_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGL7980489.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13023..13433)
                     /locus_tag="ACKB38_001761"
     CDS             complement(13023..13433)
                     /locus_tag="ACKB38_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980490.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTVIPTSYLARRRRN
                     VSEELYEEILDYLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(13426..14034)
                     /locus_tag="ACKB38_001762"
     CDS             complement(13426..14034)
                     /locus_tag="ACKB38_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980491.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14047..14505)
                     /locus_tag="ACKB38_001763"
     CDS             complement(14047..14505)
                     /locus_tag="ACKB38_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGL7980492.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4232 a         3389 c         2661 g         4353 t
ORIGIN      
        1 aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt tctatagaaa
       61 gcttacatca tcccgcccat catgcttgga tccattgctg gagctggggc tactggttct
      121 ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc tggctacaga tgctgcattt
      181 tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat catgttaacc
      241 cactcgccag ttgctgcgtt aaatcctata ccaagctcag catttttcaa acgatcgata
      301 acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc ttccaaagca
      361 cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt agcaacagct
      421 ggaatcacat tggcaagagc tgttccacca cctgcaacaa taccttcttc aacagctgca
      481 cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc agtttcagtt
      541 gcggctccaa ccttaataac cgctacacca cctgacaatt tggccaagcg ttcttgcaat
      601 ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac cgcaacacgg
      661 tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc tttgtccacg
      721 gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc aagaccaagg
      781 tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat ggctttgcga
      841 cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg aatcttgttc
      901 aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa gagtggacga
      961 ttgctttgga gaatgctttc caaaagtggc aagatttctt ggatattgga aattttcttg
     1021 tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct atctgtcacc
     1081 atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc aagctctgtt
     1141 tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt ttccattgct
     1201 tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac ttgagcgata
     1261 gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc tgcggcaact
     1321 gctgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac gtttttgatt
     1381 ccttcacgga cgatagcttg ggtcaagact gttgcagtcg tagttccgtc acctgcgata
     1441 tcattggttt tagaagctac ttctgatact aacttagcac ccatattttc aaaatggtct
     1501 tccaattcga tttctttggc aatggtcaca ccgtcattgg taatcaaggg tgaaccgaat
     1561 gacttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac agtgtctgca
     1621 aggatatcga caccacgaac catggctgaa cgggcatctg atgaaaattt aatttctttt
     1681 gacatactta ctttctcctt ctattcctca atgattgcca aaatgttagc ttcgcctacg
     1741 atgatgtact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc aactaagaca
     1801 cgatctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg aacaccttgt
     1861 ccagtagcca caacttgagc tgttttggtt ttttcttggg ctgagcctgc aaggacaaag
     1921 cctccaacag tttgttcttt ttcttctatt tttaagacca cacggtcccc taatggtttc
     1981 aacatctgat ttcctccata atgagataga tattattagc actctttgaa cttgagtgct
     2041 aattcatagt tctattgtat cacttggtca gaaataatca agaaaaaagt ctgactttct
     2101 caagataaaa agcctgagac caactcagac tttttaattc ttaaaatggc aattcttcct
     2161 cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcata gcacgttggg
     2221 cacgactttc caagagttgg aatcctgtga caagtacttc ggtcacgtag ttcatttggc
     2281 catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga ctacctttgg
     2341 ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg acaaaatcag
     2401 cttcacgttc accgttttgg tctttgtaac gacggttcac agcgatagtt gctcgcgcta
     2461 ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgt ccaatcaaga
     2521 taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa tcaaaaaaag
     2581 ttacagaaat ttgtaacttt tcgagaaaat tttttatttt ttatgaacca tgaaacctgt
     2641 cgcctgttga ttggccataa tggtcatatc tgtaatctga acacgacgag gttgactagt
     2701 cacatagact actgtatctg caatatcctg agcttgcaaa gcttctattc cttggtaaac
     2761 ggacgcagct cgttctttat caccatgaaa acgcactgta gaaaaatctg tttcgacaat
     2821 tccaggctga atggtcgtca ccttgatatc cgttgcgatg gtatcaattc gcagtccatc
     2881 tgaaaaggtc ttaactgccg ccttggtggc tgagtaaaca gctgcaccag cataggcata
     2941 aattcctgcg gttgacccca tattgataat atgaccttga ttggctttta ccattgctgg
     3001 caagaaacag cgagtgactg ccatcaaacc tttgacattg gtatccaaca tggtcagcat
     3061 atccaactct tcatagtctt gatagggagc taagccaaga gccagtcctg cgttattgac
     3121 caggatgtca atctgaccta tcgtttctaa aatatcagag cagacagtct ttaccattgt
     3181 catatccgtg acatctagga gaaaagtcca aactgtttga tttggaaaag tttctgcaaa
     3241 ctccgcctta agagcttcta gtctgtctat ccgtcgtcct gttagaacga catcctcacc
     3301 ctgctccaga taagcacgcg caatcgcttc accgattcct gatgtcgctc ctgtaatcac
     3361 aacatttttt gccatcttat ttccttctag ctggtctatc agatattaac aacttcttag
     3421 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     3481 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     3541 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     3601 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     3661 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     3721 ggatgggcta ccatctcaac aatttcacct acaacaaact taggttcctt atcattaaca
     3781 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     3841 gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc
     3901 caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc tttgcgctcc
     3961 acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     4021 tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc tgctaaaaag
     4081 tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     4141 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     4201 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     4261 aaggcaggat aaatataacg acaatcgcta caccagtctg ccacaaaaat gaagaccttc
     4321 ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg tatcataaga
     4381 cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga cggccatcct
     4441 caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga acataaaggg
     4501 tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac tcttcctgag
     4561 tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagagcaagg cttccgatac
     4621 ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc attttaacac
     4681 aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag cctcttcctt
     4741 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga agctagccgc
     4801 aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     4861 ctgttttgag gttgtggatg aagttgacgt ggtttgaaga gattttcgaa gagtattatt
     4921 cttattgcca ggcacctaag ttgccaacgt agtaactatc aggtgtgtag gtattgcgaa
     4981 gcatcttacc tgatgaagcc agataatact acttgccatt gtctttgacc caatcattcg
     5041 caatcatgga accagaagaa cttacataat accattctcc cttgtcataa acccaagtac
     5101 tgactttcat ggttcctgag caattaaagg caaaaaaact gtccaatatc attcgttttt
     5161 taaaagcatt tgacactaca tcatctacgc tgtagtacat agatacatca aacgtaaaag
     5221 ccaaaatatc gaaaaatata caacttttaa aggcagcatt tcatttttta aagtctaaca
     5281 accatagaac tgaaatcaga gcaaggcatc aatttcctac tcttagtacg tctattttga
     5341 tggttgaagt gaagattttt ttatttaacg tagacaattt ccaacttccc aaaagccaca
     5401 tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca
     5461 ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca
     5521 ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca
     5581 tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct
     5641 tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc
     5701 ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca
     5761 ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca
     5821 taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga
     5881 cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat
     5941 attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa
     6001 accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat
     6061 tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa
     6121 aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg
     6181 cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga
     6241 gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg
     6301 tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag
     6361 tcaacctcgg agcctttgac tcttgcaaga ggcgttgcaa gtctgcaatt tcatctgtca
     6421 aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta
     6481 acttcatcac atctcctttc tgactctact atatcaaagc taagttagga atacaagggc
     6541 aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg
     6601 tcagaatagg aagaaaagga atccccacac caaaccacaa gcatagccaa ccaagacatc
     6661 cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa
     6721 gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga tagttgccga
     6781 aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat
     6841 atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac
     6901 aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag
     6961 gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt
     7021 gaaactgcgt aataggaata aaagaattgg acaactagtt agtctggagc tgatatttcg
     7081 ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat
     7141 gttttcccct tggcaagcca atccactagc ttgtccaagc gacgtatttc ctgcatcaga
     7201 ggatcatcaa tttcctcaat acggacaccg caaatcttcc ctgtaattgc aattcgttca
     7261 gggacataat atggtgcttg tctaaaaaaa tctccgtaag taacatccct atccagacaa
     7321 gcaagaacat cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct
     7381 gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc
     7441 atattgtata atttctgact catacttctc tctttctatg tgtactagta taaataagaa
     7501 aaagaaggcc gtcaagcctt cttttgattt attcttctgc ttcatcttct gtaaattgac
     7561 tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct
     7621 cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg gttgacaagc
     7681 ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca
     7741 attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcggtgcat
     7801 ccttaagaag ggcacgagca atagtcaata gttgtttttg tcttacagac aaggtcacgg
     7861 tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca
     7921 cagccttact agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat
     7981 tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcat
     8041 gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata cttcccttat
     8101 caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc ccagtcggcc
     8161 caacaatggc aaccttttga ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat
     8221 tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga
     8281 ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg
     8341 ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat
     8401 ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga acagggtttt
     8461 ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg
     8521 gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac atggcataag
     8581 acatgaaaac aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa
     8641 tcacataggc cccaatccag taaatcgcca cactcaaacc acttgaaatc cccatcatga
     8701 taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc aattcatcat
     8761 ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa
     8821 tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcagcccc tgaatcaagg
     8881 actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg ataatcactg
     8941 ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga
     9001 tagccataat tgaaccacgc gttaccactt gcaagcccat agtaatcaac atttgaactt
     9061 gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg
     9121 tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag
     9181 ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca
     9241 ttcccatcat catgcttgcc gactgccaca actcatctaa attagtttct tgactaccta
     9301 gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag accgaaaagc
     9361 aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta
     9421 atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac cgagaacctt
     9481 ctcaaaaatc agtaattcat cttcatcaat gtcttccatc aactgcttgt ctatgcgttc
     9541 aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt
     9601 agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt
     9661 aaccagatta ctagcaacag gcttggtaat attgagttcc tgctcgatat ctttaatcaa
     9721 gaccaagtct tggtttttct cgcgattatc caaaaaacgc acaacctgac cttgcggccc
     9781 acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg
     9841 accaaaacgc ttaaagacta acatcggttt atccataatc tcccccttct aaataaaaat
     9901 agttctctgg agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca
     9961 agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt
    10021 ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc
    10081 aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg
    10141 gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc
    10201 tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc
    10261 gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt
    10321 gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc
    10381 tgcacggaca ttggctgctg tacggttgac gttagaagtc aaagtatcca caattagcat
    10441 agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc
    10501 tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt gtttagcacg
    10561 gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc
    10621 tacatagatt tctacaccaa attttgcata tactttagag ttagctccat ctttagccgt
    10681 tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt
    10741 taatctttct tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt
    10801 agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt ctatctttat
    10861 caacaggcac tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt
    10921 tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac
    10981 aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt
    11041 aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa
    11101 gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta gaaagctagc
    11161 ctcaggttgc tcaaaacact gttttgaggt tgcggatggg gctgacatgg tttgaagaga
    11221 ttttcgaaga gtataattta cgtgttccca agatggagaa gttagactag tacactggca
    11281 cttctaaaac attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg
    11341 ggtattagat ataaacagca aaatatttcc gatacgtgtc gttcttgaat ttccaatcat
    11401 ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac
    11461 ttttttacac attctgtttg atagattcat tttaacatca cgagcatact ccaatggaaa
    11521 tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg
    11581 caagcattct cctctcctag tcattttcta ccttatcttc tacctgagga tagagagttg
    11641 ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg
    11701 tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa
    11761 cattcccttt taaaggaata ctagtggtaa agtgagccgt taaatcctgc ccatttctgt
    11821 cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat tcattccaag
    11881 taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt
    11941 aagctgtaac cttagtctca acatagtctg tactattttg aaaggtcgca actacattgt
    12001 cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac
    12061 tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg
    12121 aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgtttcc
    12181 actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt
    12241 catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg
    12301 aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta
    12361 cagtatcttg aaacacatct cctggatttt taacagcatc tacgctgact gtataataaa
    12421 tctgcttaaa attaacaatc tgaatctgct tttcgcctga atggacagag ttaaaatcaa
    12481 tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca
    12541 tgctgtgagc cgtgattttt tcatactgca ttctagctgg gacattattg acctgaccat
    12601 aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg
    12661 ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag
    12721 tcatcggagc acgatcgacc gcaagaagag tgggattatt ctctaacaag gtctcatcca
    12781 ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac
    12841 ttgtatttgt cgacaagctc cgcttctttc tttcgataac aacaaactca tcgggtagct
    12901 gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct
    12961 ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc atcttctacc
    13021 tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt
    13081 ggttggacta agtaatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt
    13141 ctggctaaat aagaagtcgg aatgaccgta ttatccaaca taaatacctt atctaagtca
    13201 atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc
    13261 ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg
    13321 tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta
    13381 ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat caagcaagag
    13441 cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag
    13501 aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac
    13561 aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat
    13621 ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat
    13681 agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa
    13741 ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc
    13801 ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa
    13861 ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag
    13921 ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt
    13981 atcggcttct ttttttaacc agagcaaggg cgacaggtag ctggattgag acatttcctc
    14041 tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat
    14101 actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc
    14161 aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt
    14221 ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt
    14281 gataatcccc tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg
    14341 atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt
    14401 tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa aatgagctat
    14461 ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt
    14521 catttacttt gataatcata gtataacata tttgaatatc aaagtatagt aaaaactgga
    14581 ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaat
//
LOCUS       DBDESG010000057        14512 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.057, whole genome shotgun sequence.
ACCESSION   DBDESG010000057 DBDESG010000000
VERSION     DBDESG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.057"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..157
                     /locus_tag="ACKB38_001764"
     CDS             <1..157
                     /locus_tag="ACKB38_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980493.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
     gene            complement(207..917)
                     /gene="deoD"
                     /locus_tag="ACKB38_001765"
     CDS             complement(207..917)
                     /gene="deoD"
                     /locus_tag="ACKB38_001765"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGL7980494.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1571..2083)
                     /locus_tag="ACKB38_001766"
     CDS             complement(1571..2083)
                     /locus_tag="ACKB38_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGL7980495.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFCFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGSGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(2108..2857)
                     /locus_tag="ACKB38_001767"
     CDS             complement(2108..2857)
                     /locus_tag="ACKB38_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGL7980496.1"
                     /translation="MTGHSLGCYLAQIAAVEAYQKYPDFYNHVLRKVTTFSAPKVITS
                     RTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPRSSSQPSAEPME
                     NIASGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            complement(2930..3067)
                     /locus_tag="ACKB38_001768"
     CDS             complement(2930..3067)
                     /locus_tag="ACKB38_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980497.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     T"
     gene            complement(3564..4373)
                     /locus_tag="ACKB38_001769"
     CDS             complement(3564..4373)
                     /locus_tag="ACKB38_001769"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGL7980498.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(4389..4931)
                     /locus_tag="ACKB38_001770"
     CDS             complement(4389..4931)
                     /locus_tag="ACKB38_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGL7980499.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            complement(4933..6144)
                     /locus_tag="ACKB38_001771"
     CDS             complement(4933..6144)
                     /locus_tag="ACKB38_001771"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGL7980500.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIYDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFFPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(6158..6841)
                     /gene="rpiA"
                     /locus_tag="ACKB38_001772"
     CDS             complement(6158..6841)
                     /gene="rpiA"
                     /locus_tag="ACKB38_001772"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGL7980501.1"
                     /translation="MENLKKMAGITAAEFIKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(7139..7978)
                     /locus_tag="ACKB38_001773"
     CDS             complement(7139..7978)
                     /locus_tag="ACKB38_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGL7980502.1"
                     /translation="MKVIDQTLLEKVIIERSCTSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLQEQLEKAEVVLLGPGL
                     RDDTFGENLVKQVFASLKKNQILIVDGGALTILARTSLLFPSNQLILTPHQKEWEKLS
                     GIAIEKQNEGTTSSALTSFPQGTILVEKGPATRIWQVGQSDYYQLKVGGPYQATGGMG
                     DTLAGMIAGFAGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(7993..8814)
                     /locus_tag="ACKB38_001774"
     CDS             complement(7993..8814)
                     /locus_tag="ACKB38_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HGL7980503.1"
                     /translation="MILKIYNGEYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGRLLSAVKAFGRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVPGYIFDGYHAVKIKDMLNLLS
                     ELYICIIPTHNKSQFENIIPTKIQDRVYYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(8952..9809)
                     /locus_tag="ACKB38_001775"
     CDS             complement(8952..9809)
                     /locus_tag="ACKB38_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGL7980504.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(9988..10722)
                     /locus_tag="ACKB38_001776"
     CDS             complement(9988..10722)
                     /locus_tag="ACKB38_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7980505.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGDSKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQGQRTKDFLSKVL"
     gene            complement(10722..11405)
                     /locus_tag="ACKB38_001777"
     CDS             complement(10722..11405)
                     /locus_tag="ACKB38_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7980506.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            11595..11825
                     /locus_tag="ACKB38_001778"
     CDS             11595..11825
                     /locus_tag="ACKB38_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGL7980507.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            12054..14312
                     /locus_tag="ACKB38_001779"
     CDS             12054..14312
                     /locus_tag="ACKB38_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGL7980508.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPSISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         4359 a         3244 c         2614 g         4295 t
ORIGIN      
        1 ctattctaat gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc
       61 ctttggtttt cggaactttg aaaacttcaa aaaacggatt tttatcgccc tgaatatcaa
      121 aaaagaaagg acgaaatttg tcctttctca agcttagctt tttttcaacc cactacagtt
      181 gacaaagagc ccctttttgg ctataattat tctgcaatca aggtttccaa accaaccttc
      241 atcatatcag tgaaggtatt ttgacgttct tctgcagttg tgtcttcgtc tggattgacc
      301 aagctatcag agatggtcat gatagctagc gcatcaacat ggtattgggc agcaagatag
      361 taaagagctg ctgcttccat ttccacagcc ttgactcccc atttaccaag ctcgatattc
      421 ttttcaaagt aatttgagta aaagacatca gatgacaaaa cgttcccaac gtgagtagtc
      481 ataccaagtt ttttggcgat atggtaggct ttatcaagca aatcaaagct agcaatttgt
      541 ggaaaatcgt actgtggcca gtcattacga acgatgtttg agttggttgc agccgcctgc
      601 gccaaaacta attcacgaac atgaacctct tcattcaaag aacctgcagt tcccacacga
      661 atcaatttct tcacaccgta gtctacgatt aactcacgcg cataaatcga aatagatggc
      721 attcccatcc cagttcccat gacagataca cagtgaccct tgtaagtacc agtgtaacca
      781 aacatgttac gcacttcgtt aaaacaaaca gcatcatcaa ggaaattctc cgcaataaac
      841 ttagcacgaa gaggatcccc aggaagaaga attttatcag caatttcacc ctgctgagca
      901 gcaatatgga tagacataat ttatgataca aagagcgaga agaaaacgac tgaaaattag
      961 gaacctgacg agaaatcctg atttttcagt cagattatct attttccgag ttttccgctc
     1021 gtgttcaaat caaaacacac gctctacctt tctttatttt atattttata ttgagaaaga
     1081 taccaaaccc atcaaaaagc gaagggaaaa taggagttgg gcgcagtgag cgatgctcgc
     1141 tagatcaact atctttttcc cactactttt agggtggggt caattccttt cttaattttg
     1201 atttagagga gagtcgcccg tattcagttc agcgaataca gtttacccat cctttcgttt
     1261 ttgtttttag aaaagttttc tactcgtgtt caaattagaa cacgcgctct acctttctgt
     1321 ttatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttggatta tatatgtgac
     1381 tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt
     1441 tcttagtttg ctctttgatt ttcattgagt attaattttg ttctttcata gagagctacc
     1501 tgagtcctat tcaagctcag gtagtacttt cttataaact agacaaacta actgtcattc
     1561 taccatcaga ttacaagaca tcatcgtcac tcaccttgga attcaatgtc gtaccccaat
     1621 gggtaatttt acggtggggt tgagctaaaa ttggtctgtt ttcatagatt gtttgccatc
     1681 tattccatag taggcccgtc tttttctcaa tcttaactcg cagatttctc atattttctt
     1741 tgattgggag gttgaggaca aaacctgcag tctggttgcg accgcttcct tcccaagaat
     1801 gactacgaac aacttggttt ccatctttat ctactggaac ttcttcccaa gttatggagt
     1861 agcgggcaat gtaagctcca ctgtgttgaa ttgtcaatgt tttatccttc acaggagtct
     1921 gactgattgg ttgaactggc ttagaaactt gtgtcgccgt ttcagcattc gtagctataa
     1981 agcagaaaac ttcaacttct gcaagactga gggcattata gccttctagc tcaatccgaa
     2041 catagcgcgc ttttttatgg ttaatcgtaa tttgtgctga cacatcttta ggagatgtta
     2101 tacgttttca ctcaatttct ttaccagaac tgtctaaaag aatgacatca aagtttgcca
     2161 atctatcctg ggcagtgtct gttcggttgt aaatattgat ttggcgaatg gtttcttctt
     2221 tagccaaatc tacttgccac caaggcttag attggaagtt tgtatgagtg acagaattgt
     2281 gaccatagtt accatcgact ttgccatcca cagctcttct agcatctcct ccgaaagctg
     2341 tcgaactttg agtaacctgt tttcctgagg caatattttc cattggttca gcgcttggtt
     2401 gactagaaga tcgaggcaga gcctgtttct taaagaatcg cactttatcc aatttcggat
     2461 cacgataacc atgtttatcc aaggtagctt gtttaccaat attaaagtta ggaatatcat
     2521 tcattggact ttcaaaatag ccacgagaac ggtgtttaaa gcgtgaatta cctgtaaatg
     2581 taacaatatc acgattatta tgaatcaagg gagtcacaac attgtcatta tcaaccacat
     2641 aatgcttaat ttttccacta acagctaatt tacgactttc caaaccaaca tcccagaaac
     2701 cattcttagc attccaaaca gttctggaag taatcacttt aggagcactg aaagttgtca
     2761 ctttcctcaa tacatggtta taaaaatcag gatatttttg gtaagcttca accgctgcaa
     2821 tctgagctag gtaacatcca agagaatgac ctgtcatata caacttagta atacttatat
     2881 ccttggctaa ttccccaaca accttgcgga tatcatccgc ttgagctggt tatgttatct
     2941 ctacttttga ctcttccctt ttgttttcaa gaacttcact tgcttccgga agcgtttgag
     3001 gagctagtct tggctcctct ctaagaggag tttctagttt tggagcatca ttttctgact
     3061 ctttcaattc ctccttagaa tctagcacac ctattaccac ttcagatgtt acatcttgct
     3121 ttacttcatt ggctgaaatt ccagctggag ctagaaatca aagccgacta caacagatac
     3181 aactccgata cttaattttt tatgccaaag cgcataattc gttgaccaat tttcattatt
     3241 tcttatcttt cttttcgttt atttacaagc agactcaagc gaaatctcat tgtgctatat
     3301 atgataagac tgagaaattc agaaagtcaa ttcattctca tctctcctta tcatactagt
     3361 ctgcttggct aggaagctaa tgctagtatc ctagaatctt gtctttcaca atgctggcta
     3421 tcagatcaga catacctatt cttccgcttc tttcagttat aagagcagga agaagctcat
     3481 agtattcctc tgtttcttct ttaatccgga taggcagtca atcctgaaaa aacagaggca
     3541 ctcccctttt taaatctttt ttcttacaat tcagcaagaa tcgctttaag caagcctttg
     3601 aaatcacctt taacacgttc agtcacttct acaacttctt cgtgattgag ttcttcttgg
     3661 aaaccggccg caaagttagt gatacatgaa attcccagaa ctttcaagcc agagtgggct
     3721 gccacgataa cttcaggaac cgtagacata ccaactgcat ctgctcccag tgtcttatag
     3781 gaacgaattt ctgctggtgt ttcataagtc ggaccagtaa ctccgatata gacaccttca
     3841 tcaagcttga tattaagttt tttagccact tcatgggcag tggcacggta ttctggtgtg
     3901 taggccctag acatatctgg gaaacgtggg ccaaagtcat ccaagttttc acccatcaat
     3961 ggattttgcc ccgtcatgtt gatatggtct gagatagcca tcaaggtacc aggaccaaat
     4021 ccgataccgc cagctgcatt ggttacaata acaccttcac atccaagaac tttcatcaca
     4081 cgaactggga aagtcaccac ttccagagga ttcccttcat agaaatggaa acgcccttga
     4141 agagccaaga ccttgcgacc tgccagttca ccatatacca atttaccagc atgaccgact
     4201 actgttgaac ggccccagtt tggaatctca gcatagtcta ctacaactgg attttcgatt
     4261 tcttctgcca attctccaag tcctgatcca aggattagac cgaactcagg ggctgcaatt
     4321 cccttttctt tcaggaaagt agcagtttca tggattttgt ttaaaaatgt cattgtgtgt
     4381 ctcctttatt atttttttag catttgacca attttgtcaa aggttttagc attacgaata
     4441 gtaatgtggc ggtagaaagg caccttcagc aagtacttat gataggcagt cttagaatag
     4501 gattcttcag aaaatttccc ccagaaaatc ccaagttttc caaaataaag cacttcatct
     4561 ttcagctcta aactttcaac tgtcgcgatg acttggtcca catccaaacc ctcagtgtaa
     4621 aagagaaaat cttttcgtgc caagtctctg ctccaccaag ctggtagatt ttcaagttcc
     4681 gcctcaaagt cctctagact cagtaaagaa aagctctgaa taaatggata atggactgca
     4741 aagaaagtct ctagcttttc aaccaattgg gctttggaat ctatcgaagt aaagaaaata
     4801 ttgccactat tgatgtagct ctcaaccttt tccagtccca agtttgtcaa ttcttgacga
     4861 agctccgcca tgacgacctt attcttacca ccaacattga tacctctcac cagcaaagca
     4921 tagcgcgtca tcttatacca atttatctaa gaaactttcc ccaatcatag cagtttccac
     4981 accaaagtta tcggcaacag tcgctgaaat atctgcaaaa tgtcctactg gaatgagacc
     5041 atttccttta aaggcagggc tataggccaa caatggaata tattcccgag tgtgatccgt
     5101 tcctgcatac gttgggtcat ttccatggtc cgcagtaatc aagagaaggt cattctctct
     5161 catagctgcg ataatttcag gtaagcgttc atcaaactca tgcaagcaat cacggtaacc
     5221 gtgagcatta cgacgatggc cgtaaagggc atcaaagtca actaggtttg tgaatgagaa
     5281 tcctttttca aactcagcaa gtcccatagt cttcaatagt gtatcaattc catgactatt
     5341 tgacttgttg tgacccatgt catggttgat accagcaccg ttaaagatat cgttgatttt
     5401 acccacagca taagtatcga taccagcctc attcaattta tccaaaacag ttgggaaaaa
     5461 tggagataca gccaagtcac gacggtttgc cgtacgagtg aagttacctg gttcacctac
     5521 ataagggcga gcaatgatgc gaccaagaag ggcaggacgc tcaagggtaa tcgaacgagc
     5581 gtattcacag atacggtaca attcatccaa aggaataatg tcttcgtggg cagcaatctg
     5641 caaaacaggg tcagctgaag tatagataat caactctcca gtttccatct gacgtggtcc
     5701 aaaatcatag ataacagccg ttcctgaata aggtttgttg gcttcacgaa taaccttgcg
     5761 tcctgagaat tcttcgattt ttgtcaggat ttcttctggg aatccgttcc agaaagtatc
     5821 gaaaggctca gtaatgttga gtcccatgat ttcccagtgt ccagtcatag tatccttacc
     5881 aagagatact tcctctaatt ttgttgcata tccagttgga ttgctttcag ctgctacagt
     5941 cttaagagga gtttcacgag gaatatttcc aagacctatt ttagccatgt ttgggacatt
     6001 caaaccaact gtttttgaaa tgtgtcccag tgtgtcagaa gctccatctg gaacccctgc
     6061 attgacaaag ttattagcat ctggtgctgc accgattcct acagaatcca gtaccaccaa
     6121 atgaatacga ttaaatttag acatcttatg cccccttcta ttttcctttt tttgaagttg
     6181 aaatctgaac tccatctcgt ccagcaacga ttaccttatc caccatttgg ttgaataaac
     6241 catgctccac aacaccaacg acatggtcca attcttgtcc aaaagcaatt ggattttcaa
     6301 tgacatccaa ggcgaggtca atgataaaat tctgcatatc ggtcacaaaa cgttggccgt
     6361 ctttttcacg gaaacttggt ttgtagccag ctcgttcaaa atgacgaaag acctgctctg
     6421 caccatactg aaccacttct actggcaatt taaaagcacc tagtttttcg accagcttgc
     6481 tttcatccac cacccaaatg tattcttttg atggtgttgc gaccaccttt tccatgagaa
     6541 gggcaccacc accgcctttg attccattaa actgactatc cacttcatcc gccccgtcga
     6601 ctgtcacatc gacaaagtct acttggtcaa tagacttgag cgggatattg agcccttcag
     6661 cctgtttact ggtcacacta gaagtcgtca cagctgtaat ctgcaagcct tcttccttga
     6721 ttcgacgacc gatttcttcg acaaaataat aggcagtaga acctgttcct agccctacaa
     6781 ccatcccatc cttgataaat tcagcagccg tgatacctgc cattttcttc agattttcca
     6841 ctcaaacacc tccattaaag agctacctct attataccat gtaaaggctt ttatttcatc
     6901 taaaagtacc tttagaatac tgcttattta tccttgaaat tgaatttttt taataaaagt
     6961 agagctctaa caaatcaatt taagtacgga tgtaaaccaa atcaactcta acaaaaatag
     7021 atttacaaga gtaaaaaaac gaacaaattt agtttcttag caagaagaat actaaacgtt
     7081 cgttttttgt gaattaagaa taatgataca aattatcaac attttttgtc taactatttt
     7141 agacatatct tttcattact ttaggaagac aattactaat ttccgtcggc aagaccacat
     7201 aattttcttg agatagttct tgggctatgg ctgaatgaag atgggttgct actgccacac
     7261 gttcgtagag actggcctgt cgaaattggc ctgcaaatcc tgcaatcatt ccagccagtg
     7321 tatcacccat accaccagtc gcctgatagg gaccgccaac ctttaactgg taataatcag
     7381 actggccaac ttgccaaata cgagtagctg gacctttctc taccaaaatt gttccttgag
     7441 ggaaagaagt cagggcacta gatgttgtac cttcgttttg cttttcaata gcaataccag
     7501 acagtttttc ccattctttt tggtggggag ttaagataag ctggttagat ggaaacaaca
     7561 aacttgtcct agcaaggatg gttaaggccc ctccatctac aatcaaaatc tgattctttt
     7621 ttaagctagc aaagacctgt tttacaagat tttctccaaa cgtatcgtct cgtaaaccag
     7681 gccccagcaa gacaacttct gccttctcca attgctcttg taacaattgc tgatcttgca
     7741 gagaaaaggc catagcctca ggcaaatggc tgtgtagagc agggatattt tccctgtccg
     7801 ttccaacggt taccaatcct gcaccgcttt ttacagctgc taaagcagcc atgatgatgg
     7861 caccaccata aggataagtc ccaccaagca acagcagacg accgtagtct cctttatgac
     7921 ttgtacaaga acgttcaata atgacttttt ctagtaaggt ttgatcaatc actttcatcg
     7981 ttttttcctc aactatcctt tatctgattg ttttaaaaca acttgataga ctttcttagt
     8041 ccactcttct aagtcttctc cagcatagtc aagataatac accctatctt gtattttggt
     8101 tggaataata ttttcaaatt ggctcttatt atgagttgga ataatgcaaa tatacaactc
     8161 acttaataaa ttaagcatgt ccttaatttt tacagcatgg taaccatcaa aaatataacc
     8221 aggaacttta atcaaatctg tatagataaa atgaaaactt actccaggaa taaacttgtc
     8281 ttgtaattct ttctctgaag gagctcgacc taataaacgc tccatcgcca atcgataacc
     8341 agaacttgta tttgaccacc ctaacatgac atagtcaaaa taatctatcg gatcagatgc
     8401 agcatttcga ctatccaaaa ccaactcctc agcatctcgc ccaaaggctt tcacagcaga
     8461 taaaagacga ccagttttaa aaatagaaat cgctgcatct atagtcgtag tatgactaca
     8521 gtagtctgaa gtcactaacc tcttatgtag gtcataagtc gaggctactc ttttatctgt
     8581 aggagtaaat ggtggcagaa agggatgctg acagatgtaa tctaaaattt ctttttttaa
     8641 acaagaatca gcacactcaa tacttgtttg acgtttatga agtttttcgt aagctataaa
     8701 tttagcaatt ttttctaatt cccactcttg aatatttgga taatcaatta gtgctaagta
     8761 gtatattcca tcccattgta aactatattc cccattataa atttttaaaa tcatctaatc
     8821 acctcactcc attatacaac aaaaaggagg cgcaaacctc cttttataaa cttatttcta
     8881 aaatttaata ttcatttcta tcattttaga tatagctata gaaaatacac tttcctcaga
     8941 aaatttttat cttattttct atccaatgtc ctaagtgctg cctgataggt ttgctccatc
     9001 agcatagtaa tggtcatagg accgacacct ccagggactg gcgtaatgtg gctagcaagt
     9061 ggggcaaccg cctcataatc aacatcccca cagagcttac cattttcatc gcggttcatc
     9121 ccaacgtcaa tgactaccgc acctggtttg acaaagtcag cagtcacaaa cttggcacga
     9181 ccgattgcaa caaccagaat atctgctttt gcagccacct tggaaagatt atgagtacgt
     9241 gagtgagtca aggttactgt tgcattcttt gccaaaagaa gctgggccat aggttttccg
     9301 acaatattgg atcgaccgat gacgactgca tttttacctt ccaagtcaat cccatattca
     9361 tggaacattt ccataattcc tgccggtgtc gaaggaatca tgactggatg accagaccaa
     9421 agacgcccca tgtttagagg atggaaacca tccacatcct tttctgggtc aatagccaat
     9481 agaaccgcct cttcatcaat gtgttttggt aatggcaact gaaccaaaat cccatgccaa
     9541 gctggatcct gattgtattt agcaatcagg tctaacaatt cctcttgagt aatggtctct
     9601 ggaacccgta ctacttcgct acggaaacca gccgcaaggg ctgacctctc cttgttgcga
     9661 acgtagactt ggctggctgg attgtcccca accaaaatca ctaccaaacc aggcactaga
     9721 cctgtttctt cctttaattt tgcagtcttt tcagccaact gcccctgcaa tttggccgct
     9781 aaagctttcc catcaataat ctgtgtcata tcacttctct tttcttacaa atatctttaa
     9841 ttataccaaa aaatacaatg tagctctaac acttctttcc taggcaatcc tatagcttta
     9901 aactataaga aaaagctctg gttgagcttt tcactaatct tctctttaac gtttaaagct
     9961 ggctacaaat agctaaacaa agctaactta taaaacctta ctcaagaagt ctttagtcct
    10021 ttgtccttgg gtttgttcaa aaatctgctc aggtgttccg tcttcaacaa ccacaccgtc
    10081 tgccataaag atgacacgat ctgccacctc acgggcaaat cccatctcat gtgttacgat
    10141 aaccatggtc attcctgact tggctagatc ttgcataaca gccagaactt ctccaaccat
    10201 ctcaggatct agggctgaag ttggctcgtc aaagagcaaa acatctggtt ccatagccaa
    10261 cccacgcgcg atggcaatcc gctgttgctg gccacctgac aaactctgtg gataagcgtc
    10321 tgccttatct ggcaaaccaa ctttttccaa aagttcctga gctcttttct ctgcaacggc
    10381 cttactgtca cctttggtct tgatagggga caaggtgata ttttccatca cagtcatatt
    10441 aggaaagaga ttgaattgtt gaaaaaccat gcccatcttc tcacgcatgg caaacaggtc
    10501 attcttcttg tccgtaatat cgactccctc aaagataacc ttccccttgg ttgcttcttc
    10561 caacaaattc atagagcgaa gcaaggtaga tttcccgctc cctgaaggac cgatgataac
    10621 gacaacttct cctcttttaa tctcgaggtt gatgcccttc aatacttcat tctttccaaa
    10681 ggatttatgt aaattttcaa tttttatcaa ggtttctgtc attatttctt atctccttgt
    10741 cccatatgtt tttcaaaagc tttcaaggct actgtcagaa tagaggtcat aatcaagtag
    10801 taaaatgctg caaataaaag tggtgttaaa ggtagatagg ttgttgtaga aactgttgta
    10861 gccccattcc acaactccat gaccccaata gctgataaga gggagctgtc cttgataatg
    10921 gtgataaatt cgttccccaa tgctggcaag atatttttga ctgcttgtgg caaaatcaca
    10981 taacgcatcg catttttagg acgaatccct agcgaataag ccgcttctag ctgacctttt
    11041 ggaaccgcat tgattccggc acgaacagtc tccgaaacat aagcaccact attcatagag
    11101 ataatcaaaa tccctggaat cagacgcgaa aaatcaacac ctaaaattcc aatctgaata
    11161 gtcggagcat tgatatgcat aagagcaaag gcaatcataa tttgaaccat catcggtgtc
    11221 ccacggaaaa tccaaacgta caagttggcc aaccaaacaa gcggtttaaa ctttgaacgt
    11281 tgcccaaaag ccaagacaac acccaaaata gttcccaaaa agataacaca gatagaaata
    11341 agaatcgtca caacagcccc atagttaaaa taaggtaaat acttaggtaa aaaagaaaaa
    11401 ttcatagtca cactgctttc taaaatagaa tactgtatga ttataacatg ttttgaatta
    11461 aaatttaaat tttttatcca atttatttaa aaatatttaa actagcagaa ttagcgagaa
    11521 atcttagttt tttctagtca agttggcctc ctttatggta aaatagtaac gataagaaat
    11581 gaaggagaat caaaatggaa tcacatttgg ttagaatcat caaccgcctt gaagcaatgg
    11641 caaacgatgg cggaaattta aaacgtaatt ttgaacgcga aggagttgtt gttgcagaag
    11701 ttgcgtacag ccacgacgaa gaaaacggcc caatctttac ccttcgtgac gtagaagatc
    11761 gcgaaacgta tacgtttgat agcattgact taatcgcaat ggaaatttac gaacttctct
    11821 actaatctaa aacaagctgg gattgtccct gcatgtctaa gtaaaatcct ttttgtctca
    11881 caaataggat ttttttatag tccaaattca caaaaatttt cattgtaaca acttctcaaa
    11941 gcttcaatct ttttacttgc tttacacccc aatcctgtta taatgagagt atagaatttg
    12001 actatttttg acctttaaac aagtcaggct aaagaaagaa atgaggtaga tgtatgcttt
    12061 gtcaaaactg taaaattaac gactcaacaa ttcatcttta caccaatctc aatggaaaac
    12121 aaaaacaaat tgacctctgt caaaactgct ataagattat caaaacagat cctaacaata
    12181 gcctcttcaa aggtatgacg gatctgaaca atcgtgactt cgatcccttt ggtgatttct
    12241 tcaatgatct aaacaatttc agaccttcta gcaatactcc tcctattccc ccaacccaat
    12301 caggtggagg ttacggtgga aacggcggtt atggttccca aaatcgtgga tctgctcaaa
    12361 ctccgccacc tagccaagaa aaaggcctgt tggaagaatt tggtattaat gtaactgaaa
    12421 ttgcccgtcg tggagacatt gaccccgtta ttgggcgcga cgatgagatt atccgtgtca
    12481 tcgagattct caatcgtaga accaagaata atcctgtcct tatcggtgaa cctggtgtcg
    12541 gaaaaacggc cgttgtcgaa ggtctagctc agaaaattgt cgatggcgat gtgccacata
    12601 aactccaagg taaacaagtc atccgtctgg atgtggttag cttagttcaa ggaacgggga
    12661 ttcgaggaca atttgaagaa cgcatgcaaa aactcatgga agaaattcgc aaacgtgaag
    12721 acatcatcct ctttatcgat gaaatccatg aaattgttgg tgctggttct gcgagtgatg
    12781 gtaatatgga cgcaggaaat atcctcaagc cagcccttgc tcgtggagaa ctgcaactag
    12841 tcggtgctac taccctcaat gaataccgta tcattgaaaa ggatgctgcc ctcgagcgtc
    12901 gtatgcagcc tgttaaagtc gatgaaccaa cggtggacga aacaatcact attctcaaag
    12961 ggattcaaaa gaaatacgaa gattaccacc acgttcaata tacagatgct gcgattgaag
    13021 cagctgcaac tctttccaat cgctacatcc aagatcgctt cttgcctgac aaggccattg
    13081 acctcctaga tgaagctggt tctaagatga acttgacctt gaattttgtg gatcctaaag
    13141 taattgatca gcgcttgatt gaggctgaaa atctcaagtc tcaagctaca cgagaagaag
    13201 attttgagaa ggcggcctac ttccgcgacc agattgccaa gtataaggaa atgcaaaaga
    13261 aaaagatcac agaccaggat actcctagca tcagcgagaa aactattgag cacattatcg
    13321 agcagaaaac caatatccct gttggtgatt tgaaagagaa agaacaatct caactcatcc
    13381 atctagccga agatctcaag tctcatgtta ttggtcaaga tgatgcagtc gataagattg
    13441 ccaaggctat tcgccgtaat cgtgtcggac ttggtacccc taaccgccca atcggaagct
    13501 tcctcttcgt tgggccaact ggtgtcggta agacagaact ttccaaacaa ctggctatcg
    13561 aactttttgg ttctgctgat agtatgattc gctttgatat gagtgaatac atggaaaaac
    13621 atagtgtggc taagttggtc ggcgctcctc caggttatgt tggctatgat gaggctggtc
    13681 aattaactga aaaagttcgc cacaatccat attctctcat ccttctcgat gaagtggaaa
    13741 aagctcaccc agatgttatg cacatgtttc ttcaagtctt ggacgatggt cgtttgacag
    13801 acgggcaagg acgcaccgtt agcttcaagg atgccatcat tatcatgacc tcaaatgcag
    13861 gtacaggaaa gaccgaagct agcgttggat ttggtgctgc tagagaagga cgtaccaatt
    13921 ctgtcctcgg tgaactcggt aacttcttta gcccagagtt tatgaaccgt tttgatggca
    13981 ttatcgaatt taaggctctc agcaaggata acctccttca gattgtcgag ctcatgctag
    14041 cagatgttaa caagcgcctc tctagcaaca acattcgttt ggatgtaact gataaggtca
    14101 aggaaaagtt ggttgaccta ggttatgatc caaaaatggg agcacgccca cttcgtcgga
    14161 ctattcaaga ctatattgag gacacaatca ctgactacta ccttgaaaat ccaagcgaaa
    14221 aagatctcaa agcagttatg actagcaagg gaaacattca gattaaatct gccaaaaaag
    14281 ctgaagttaa aagttctgaa aaagaaaaat aaatcctata aaaaaggagt agaaaatgaa
    14341 atttttctgc ttcttttttt actaaaataa ctgtaatttc ttgacagctt gccctttgtc
    14401 cattatgata tatagtagac tgaatctgaa atagtacgaa acaattgcta aaacatttat
    14461 agaaattaat tttactttcc caatcgattt gttctcatct tatttcaatc tg
//
LOCUS       DBDESG010000058        14274 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.058, whole genome shotgun sequence.
ACCESSION   DBDESG010000058 DBDESG010000000
VERSION     DBDESG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.058"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..2117
                     /gene="spuA"
                     /locus_tag="ACKB38_001780"
     CDS             <1..2117
                     /gene="spuA"
                     /locus_tag="ACKB38_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGL7980509.1"
                     /translation="GKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIE
                     KLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGM
                     YSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDAD
                     GTPRTSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKA
                     ARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNE
                     GQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKD
                     PSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPN
                     KSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIA
                     LRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADE
                     KAREFNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQ
                     NGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQ
                     ATADSQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLL
                     FAGISLLALLGLGFLLKNKKEN"
     gene            complement(2217..3266)
                     /locus_tag="ACKB38_001781"
     CDS             complement(2217..3266)
                     /locus_tag="ACKB38_001781"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGL7980510.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(3385..5193)
                     /gene="glmS"
                     /locus_tag="ACKB38_001782"
     CDS             complement(3385..5193)
                     /gene="glmS"
                     /locus_tag="ACKB38_001782"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGL7980511.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(5386..6765)
                     /gene="bglA"
                     /locus_tag="ACKB38_001783"
     CDS             complement(5386..6765)
                     /gene="bglA"
                     /locus_tag="ACKB38_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGL7980512.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(6864..8717)
                     /locus_tag="ACKB38_001784"
     CDS             complement(6864..8717)
                     /locus_tag="ACKB38_001784"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGL7980513.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDDYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(8730..9989)
                     /gene="rseP"
                     /locus_tag="ACKB38_001785"
     CDS             complement(8730..9989)
                     /gene="rseP"
                     /locus_tag="ACKB38_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGL7980514.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PEMAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(10011..10814)
                     /locus_tag="ACKB38_001786"
     CDS             complement(10011..10814)
                     /locus_tag="ACKB38_001786"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGL7980515.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIILMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(10823..11581)
                     /locus_tag="ACKB38_001787"
     CDS             complement(10823..11581)
                     /locus_tag="ACKB38_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGL7980516.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(11812..12384)
                     /locus_tag="ACKB38_001788"
     CDS             complement(11812..12384)
                     /locus_tag="ACKB38_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGL7980517.1"
                     /translation="MNTVKNKQEILEAFRESPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEELDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(12365..13363)
                     /gene="ruvB"
                     /locus_tag="ACKB38_001789"
     CDS             complement(12365..13363)
                     /gene="ruvB"
                     /locus_tag="ACKB38_001789"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGL7980518.1"
                     /translation="MSRILDNEMMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            13498..13590
                     /locus_tag="ACKB38_001790"
     CDS             13498..13590
                     /locus_tag="ACKB38_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGL7980519.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         4262 a         3374 c         2686 g         3952 t
ORIGIN      
        1 atggtaagat tcacaatttc aagactcgtg aagacgccgt tatctacgaa gctcatgtgc
       61 gtgatttcac ttcagatcct gccattgcaa aagacttgac caaaccattt gggacttttg
      121 aagccttcat tgaaaaacta gactatctca aagacttggg tgtaacccat atccagctcc
      181 ttccagtctt gtcttactac tttgtcaatg aattgaaaaa ccatgaacgc ttgtctgact
      241 acgcttcaag caacagcaac tacaactggg gatatgaccc tcaaaactac ttctccttga
      301 ctggtatgta ctcaagcgat cctaagaatc cagaaaaacg aatcgcagaa tttaaaaacc
      361 tcatcaacga aatccacaaa cgtggtatgg gagctatcct agatgtcgtt tataaccaca
      421 cagccaaagt cgatctcttt gaagatttgg aaccaaacta ctaccacttt atggatgccg
      481 atggcacacc tcgaactagc tttggtggtg gacgcttggg gacaacccac catatgacca
      541 aacggctcct aattgactct atcaaatacc tagttgatac ctacaaagtg gatggcttcc
      601 gtttcgatat gatgggagac catgacgccg cttctatcga agaagcttac aaggctgcac
      661 gcgccctcaa tccaaacctc atcatgcttg gtgaaggttg gagaacctat gccggtgatg
      721 aaaacatgcc tactaaagct gctgaccaag attggatgaa acataccgat actgtcgctg
      781 tcttttcaga tgacatccgt aacaacctca aatctggtta tccaaacgaa ggtcaacctg
      841 cctttatcac aggtggcaag cgtgatgtca acaccatctt taaaaatctc attgctcaac
      901 caactaactt tgaagctgac agccctggag atgtcatcca atacatcgca gcccatgata
      961 acttgaccct ctttgacatc attgcccagt ctatcaaaaa agacccaagc aaggctgaga
     1021 actatgctga aatccaccgt cgtttacgac ttggaaatct catggtcttg acagctcaag
     1081 gaactccatt tatccactcc ggtcaggaat atggacgtac taaacaattc cgtgacccag
     1141 cctacaagac tccagtagca gaggataagg ttccaaacaa atctcacttg ttgcgtgata
     1201 aggacggcaa cccatttgac tatccttact tcatccatga ctcttacgat tctagtgatg
     1261 cagtcaacaa gtttgactgg actaaggcta cagatggtaa agcttatcct gaaaatgtca
     1321 agagccgtga ctatatgaaa ggtttgattg cccttcgtca atctacagat gccttccgac
     1381 ttaagagtct tcaagatatc aaagaccgtg tccacctcat cactgtccca ggccaaaatg
     1441 gtgtggaaaa agaggatgta gtgattggct accaaatcac tgctccaaac ggcgatatct
     1501 acgcagtctt tgtcaatgcg gatgaaaaag ctcgcgaatt taatttggga actgcctttg
     1561 cacatctaag aaatgcggaa gttttggcag atgaaaacca agcaggacca gtcggaattg
     1621 ccaacccgaa aggacttgaa tggactgaaa aaggcttgaa attgaatgcc cttacagcta
     1681 ctgttcttcg agtctctcaa aatggaacta gccatgagtc aactgcagaa gagaaaccag
     1741 actcaacccc ttccaagcct gaacatcaaa atgaagcttc tcaccctgca catcaagacc
     1801 cagctccaga agctagacct gattctacta aaccagatgc caaagtagct gatgcggaaa
     1861 ataaacctag ccaagctaca gctgattcac aagctgaaca accagcacaa gaagcacaag
     1921 catcatctgt aaaagaagcg gttcgaaacg aatcggtaga aaactctagc aaggaaaata
     1981 tacctgcaac cccagataaa caagctgaac ttccaaatac aggaatcaaa aacgaaaaca
     2041 aactcctatt tgcaggaatc agcctccttg cgctccttgg tctcggtttc ttactaaaaa
     2101 ataaaaaaga gaactaaact agccctccta tagaaaaatc ccccaagcat tatagctcgg
     2161 gggattaatt tttgtacaat atttgttgtc ctaataaact tgattaggat tttttattaa
     2221 gcctctttca tagcaaaata agctcgtact ttgggtgcaa cttgtgttcc gaagagttca
     2281 atagctctca gaacctggtc atgaggcata gaaccaagcg gtagatgaag catgaagcgg
     2341 tccaatccta aatcctctat catgcgaatc aatttttcgg ccacctgatc tggattgcca
     2401 acaaacatgg cgccatttgg ccctacctgc tccaaatatt gctcataacg caattcctgc
     2461 cagtgcggac ggtctttgga aatagcatcc accacttgct tagtcggatg gaaataatct
     2521 ttcaccgcct gctcaccatc ttccgcaatc cacccccaag aatgggctcc cactttcaag
     2581 tctttgtcag catggcccgc ttcgcttcca atctcacgat aagcctgaat caacttttta
     2641 aaataacgtg gattaccacc aataatagca tatacaatcg gtagaccagc ctgagcaatc
     2701 ttcactgttg attcgacatg accacctgta gctatccaca agggcaattt gtcctgaact
     2761 ggacgaggat aaacttcttt accagcaatc gtttgagtca atcgaccttg ccagtctaac
     2821 ttggtctttt cattgactaa ctgaagcaag tctaatttct catcaaaaag agagtcgtag
     2881 tctttcaagt cataaccaaa cagagggaaa gattccgtga aagagcccct tccagccata
     2941 atctccgatc gtccatttga caaagcatcg atagtggcat actgttggaa caaacgaatc
     3001 gggtccatgc ttgacagaat gctgactgca ctggtcaaac ggattttctt ggtattgact
     3061 gccccagcgg ccagaacaat ctctggggct gatactgcaa aatccgcccg atggtgctca
     3121 ccaatcccat atacatccaa accaaccttg tcagccagct caatctctgc caccaactgg
     3181 cgaatgcgtt cagcatgact gtaagtttgt ccagtccctt caagctccgt tatttcccca
     3241 aatgttgaaa ttcccaattc taccattgtg attctcctta tctatctctg tacttcaatt
     3301 tgaaaaatta ttctaacacg aatcttgagt acaagcaacc gatttgctca ttagaaaaag
     3361 cctagataac tagacttttt tagcttattc taccgttact gacttggcaa ggttacgtgg
     3421 tttgtccaca tcgaggccac ggtggagggt tgcaaagtaa gcgactaatt gcgttggtac
     3481 gaccattgaa attggtgaga ggtatggatg tacggtcgta aggacgatat cgtcggtatc
     3541 tttggctaca ttctcttctg cgatagtgag gactttggca ccacgggctg cgacctcttg
     3601 gatatttcca cgagtatgat tggcaagaac tggatctgac aagagagcca aaacaggcgt
     3661 tccttcttca atcaaggcaa tggttccgtg cttgagttct cctgcagcaa agccttcaca
     3721 ctggatataa gaaatctctt tgagtttgag acttgcttcc atggctacgt agtaatcttg
     3781 accacgtccg atgtaaaagg cgttacgagt tgtttcaaga agttcacgaa ccttgacttc
     3841 aatggtttct ttctctgaaa gagttgattc gatagactga gctacgattg acaattcatg
     3901 aaccaggtca aaggcttgcg ctttagcatt accatttgct tctccgactg cttttgcaag
     3961 gaaggcaagg gctgcgattt gcgctgtata ggctttagtt gatgccacgg caatttcagg
     4021 acctgcgtga aggagcatgg tatagttggc ttcacgtgag agggttgaac ctggaacgtt
     4081 tgtcactgtt aagcttggaa ttcccatttc attagccttg accaaaactt gacgactatc
     4141 cgctgtttca ccagattggc tgataaagat gaagagtggt ttcttgctga gaagtggcat
     4201 accgtagccc cactcagatg agattccaag ttcaactggt gtatctgtca attcttccaa
     4261 cattttctta gaagcaaatc ctgcatggta agatgttcca gctgcaagga tgtagatgcg
     4321 gtctgcgtct tgaacagcct taatgatatc tgggtctacg acaacttgac cagcctcatc
     4381 tgtgtaggct tggatgagtt tccgcataac agttggttgc tcgtcaattt ccttgagcat
     4441 gtagtaaggg taagttccct taccgatatc tgacaagtca agttcagcag tgtagctagc
     4501 acgctcacga cgatttccat catagtcttg aacttccaca ctatcagcct tgacgattac
     4561 caactcttgg tcatggattt ccatgtattg gttagtttca cgaatcatag ccatggcgtc
     4621 tgagcagacc atgttatagc cttctccaag accaatcaaa agtggtgatt tatttttagc
     4681 tacgtagatg acttcaggat cttgtgagtc aaccaaggca aaggcataag aaccacggat
     4741 gatgtgaagg gcttttttga aggcttcaag aactgagagc ccttcttctt ccgcaaattt
     4801 tccaatcaaa tgaacggcta tttcagtatc tgtctgcccc ttgaagtggt gacctgcaag
     4861 gtattcttcc ttgatttcaa gatagttctc aatcacccca ttatgcacca agacaaaacg
     4921 ttccgtctca gagcggtgtg ggtgagcatt gtcctcagtt ggttttccgt gagtagccca
     4981 acgagtatgt ccgataccag ttgttccctc aacaccagct gtcttggcag acaattctgc
     5041 aatacgacca accgccttca ccaaatggtt atcagcacca tctaggacaa aaattcccgc
     5101 agaatcatag ccacggtatt caagcttttc aagcccttga atcaaaatat cagttgcatt
     5161 tgtgtttcca acaacaccaa caattccaca catagtatat acgacacagg caagctgtgc
     5221 tttctcctta aaattggtat agtctaattc atcttttata gaatcagcaa aaacagtata
     5281 tacttgtttc tttcacttgt caagagtaaa aattggtata gttcaaatta agctcctgta
     5341 agcataaaaa ctctgaccga ttgggataat cagtcagagt cctttttaaa atccattatt
     5401 atcgcttaat tctttgaacc agtggcctga tttcttcaga cgacgttctt gcgtttccaa
     5461 gtctaattcg accaaaccat agcgattttt atagctgttg agccatgacc agcagtcaat
     5521 aaaggtccaa atcaagtagc ccttacagtt ggcaccatct tcaatggcac ggtgaagttc
     5581 acgaagatga ccttttacaa agtcaatacg gtaatcatct tgaatcattc catcttgacg
     5641 gaatttttct tccccttcaa cacccatacc attctcagtc aacatccact caatattgcc
     5701 ataattttcc ttgatatttt gggcgatgtc ataaatccct tgctcataaa tctcccaacc
     5761 acggtgagaa ttgattttac gtccaggcat cacataaggc tcgtaaaaat gttctggtaa
     5821 gagtggactc tctggatgct tagcaaatcg aggagccata acacgcaaag gttgatagta
     5881 gttcacacca aggaagtcca ccgtattatc acgaatgagt tccaactctt cctctgtagc
     5941 atcaggtaaa agaccgtgtt catgcaagat ttctaccaac tcctgtggat aagtccccaa
     6001 gacagatgga tctaagaaag attgggcctg aaaaagggcc gcaatacgag ctgccttgac
     6061 atcagcagga tgctggctac gtggataagc cggtgtcaag ttgaggacaa tcccaatctt
     6121 ggaatcaggc aaaagttcat ggcaagcctt aacagcccgg ctgctggcca attgtgtatg
     6181 ataggctacc ttaacagctg cctctgcatc caccttatgt ggataatggg catcataaaa
     6241 ataaccaaat tctacaggaa cgatgggctc gttaaaggta atccattgat ccactaaatc
     6301 tccataagtc tcaaaacaaa aacgagcata gtcttcatag gctgagactg tcgccttatt
     6361 ttcccaacca tcaccatcct cttgaagggc aaaaggtaaa tcaaaatgat agagattgac
     6421 taacagacga attcctttag ccttaatagc ctcaaagacc ttacgataaa aatccacacc
     6481 ttgagtgttg acttttccac agccttgtgg aaaaatccgt gaccactgaa tagaagtccg
     6541 aaaggctgtg tgaccagtct ctaacaaaag ctcaatatcc cgctcccaat tttcataaaa
     6601 agtcgatgtc ttatctgaac caatcccatt atagtaacga tttggctcca cttggaacca
     6661 gtaatcccag agattgtctc ccttaccgtc accagctaca cgtccttctg tctgcggtcc
     6721 agaagtagag gatccccaga caaaatcctt tggaaatctt agcatacatt tacctcttta
     6781 tctactcatt tctcccatta tacagaaaaa acaaggtaaa aactagttac attttttcct
     6841 tgtttttctt ctgattatag tttttatttc ttgcttagga tttcaagcgt ttcaagcacg
     6901 ttatctgcat gaacctcaat ggtgtcacca gttgccttga tcttaacttc tacaatgcca
     6961 tcggccgctt ttttaccaac agtgatacgg attggaagac caatcaagtc actatcgcta
     7021 aatttaacac cgacacgttc gttacggtca tctgtcaaga cttcataacc agctcccatc
     7081 aagcttgctt caagtttttc tgtcaaggct tgcgcttctt catccttgac attgacagta
     7141 atcaaatgca catcaaatgg tgccaattct ttagggaaat tgattcccca agcgtaacgg
     7201 tattcacctt ttggcgtttt gttaacaaag aggcgagcgt gttgctccat cactgctgaa
     7261 agaagacggc tgacaccgat accgtaacat cccatgatga ttggcacagc acgaccattt
     7321 tcatccaaga catctgctcc catgcttgct gaatagcgag ttccgagttt gaaaatatga
     7381 ccgatctcaa taccacgcgc aaagttaagg acaccttgtc catctgggga aatttcaccc
     7441 tcacgaactt cacggatatc cacatattct gcagtaaaat cacggcctgg gttcacacca
     7501 gtcaagtggt agtcatcttc gttagcaccg acaactgcat tgcgaacatc ttgtacctta
     7561 cgatctgcaa taattttaat attctctggc aaaccaactg gtccaagtga accaaatcct
     7621 gcttgaacaa cattcgccac ttcttcttcg ctagcaacgt caaagaaatc tgctcccaag
     7681 tgatttttca acttgacttc gttgagttgg tcatttccaa ctagaagggc tgcaacaagc
     7741 tcaccatctg caatgtagaa gagggtttta atcgtttgtt cttctggaac attgaggaag
     7801 gctgcaactt catcaattga tttaacatct ggcgttgcaa cacgagtaac ttcttcttca
     7861 gcgacaacac ggttgcttgg tttgtactcg tttgttgcca tttctaagtt agctgcatag
     7921 ctagactcac ttgagtaagc aatggtatct tcaccagaga ctatccattt gagcaattct
     7981 gccttgattt cttcttgcac ttctgcagga atttcgtcaa atgaggcaac tgacttgtcc
     8041 aagacaaccc agcggtcaag gtctgtacga gcagatgtaa tggccataaa ttcttggcta
     8101 tccttaccac ccatggctcc accgtcacca ataatagcct tgaagtctaa accactacga
     8161 gtgaaaatac gctcataggc tgctttgtac tcatcataaa cactatccaa actatcatag
     8221 ttagcgtgga aactataagc atccttcatg ataaactcac gtgtacgaag aagtccatta
     8281 cgcgggcgtt tttcatcacg atacttgggc tgaatttgat aaaggttgag tggcaattgc
     8341 ttgtaagatt taacagaatc acggacaata gctgtaaagg tttcttcgtg agttggacct
     8401 aagataaagt ctgatttttc acggtttttt agtttgtaaa ggtcttcacc ataggtttcg
     8461 taacgacctg attcacgcca caattctgca ctaagaaggg ctggagccaa catctcaaca
     8521 gcaccaatct tttcgaattc ttggcgcatg atgtttttag ctttttcaat cacacggttg
     8581 gcaagtggta gataagaata aacacctgct gaaacttggc gaacataacc agcacgcaac
     8641 ataagagcat ggctgataac ttgagcatcg cttggcattt cgcgaagcgt tgggataggc
     8701 attttacttt gtttcataat attcctcgat tatctaaaaa agagtcgcat aatgtcattc
     8761 caagtcacag caatcatcaa gacaaccatg atgaccactc cggccaaggt gacataggtt
     8821 tcaatttctt gtttcaatgg tttgcggcgg atggcttcta ggatattgag cacaatctta
     8881 ccaccatcca aggctggaat cggaataaga ttaaaaatcc caatattgat ggaaatcatt
     8941 gccaagaagt acaagatatt ttcaattcca tttttagcag catcactact tgccttaaag
     9001 atagcaacag gtccacccaa cttgttcaaa tctggttgga aaatcagatt tttcagagct
     9061 gagagaattc ggagagctga gtcagcagca gttgtaaaac cacctacaaa catggataga
     9121 aaatctgact taacccccgg ttgaacacct agaaggtaac gaccttgact atctttgggt
     9181 gtaacagtga cttgtttgtc actccccttt tcagaaatag tcacatccaa agtcggtgcc
     9241 gtcttatctt tggtttctgt ttccacagct tggatcaagc tttcccagtt gctaacctca
     9301 tgtgagccaa tcttggtaat ttgtgccatt tctggtactc ctaccttggc caaggcacct
     9361 tggggcatga tatggaactg attggtatca acatctctga caccaccctg cataaagatt
     9421 aaaacccaaa aaacaacgac acctaagata aaattgttca taggacctgc aaaattggta
     9481 atcagtttgc cccagatagt cgcattttga tattgaacat ctaaaggtgc aatccgaacc
     9541 tcagtaccat ctgcttccac aaccgttgca tcgtgatcca ctgcaaatgt tttttcttct
     9601 tccagaacca atcctttgat aaagagcttg tcttcaaaat caaactgggt cacctgcata
     9661 gggagggctg tttgatccaa ttttttacct gagagattga tgcgtttaac cttaccatca
     9721 tcagcaagtg tcaaactaac aggcgttcct gtcttgattt cagttgtatc atcaccccaa
     9781 ccggccatgc ggacatagcc acccagaggc aagattcgaa tggtataggc cgttccatcc
     9841 ttgccaatgt gagcaaaaat tttaggtccc ataccgatgg caaattcacg tactaaaatc
     9901 cctgatttct tggcaaagta gaagtgaccg aactcgtgca ccactacaat aatcccgaaa
     9961 accagaataa aggttaaaat tccgagcata gcgtttcctc cgtcttttga ttaaaagagt
    10021 ccaaataagt gcatgattgg aaatacaagc aacatactat cgaaacgatc caaaacacca
    10081 ccatgtccag ggataaattt cccagaatcc ttaacaccaa aatgacgttt gatcgaactt
    10141 tctagtaaat caccaaattg tccagcaatg ctaaagaaaa tagcaaagac tgacatcttg
    10201 taaattccat atggaagagc aactgtactg tcaactatca taaggataat ggttactaaa
    10261 attgctccta aaataccacc caaggcaccc tcaagggttt tattaggcga tacccttggt
    10321 gctaactttc gtttcccata gttcatccca acaagatagg caccactgtc tgtcgcccag
    10381 acgatacaca aggctaagag agccttgtcc aaacctgcaa cacgagcatc tagtaaagca
    10441 ttaaatccaa agcccacgta gaagctcata gcaagaggga aaaccgcatc ctcaatcgta
    10501 taagacttgc taaaaacggt cgttcctaac atgattgaaa tcaaaacact ataggcaacc
    10561 acattcccat caactggcaa aaaagtcagg taattctcca agggaatggt caatgcaaag
    10621 gttgcaaaga gggtcaagag gccctccatc gtcatggtct ctagacctct catcttcaaa
    10681 agttcatgca tggctagcat ggctatgatt ccgattgcta tctgaagcaa gaggccccca
    10741 atcattaaaa ttggtaggaa aatagccagg gcaatccctg caaacaaggt tcttttctgt
    10801 aaatcctggg tcatatttcc tcctaaactc ctccaaatcg gcgatgacga cgattatagg
    10861 caagaatagc ttcctgcaag gccgcttcgt caaaatcagg ccataaggtg tccgtaaaat
    10921 aaagctcact ataggctccc tgccatggaa ggaaattgct caaacgtaat tctccactag
    10981 tacggataat caagtctggg tctcgtaagt ccttaggcaa atgctgagta aagagatagt
    11041 taccaatcaa ttcctctgtg atgtcacctg ggttgatttt ggcatctaaa acatcctggg
    11101 aaatcaactt aagcgcctgt gtaatctcag cacgtccacc atagttaaga gcaaaattaa
    11161 gaatcaatcc tgtgttgttc ttagtcaatt cctcagcctt ggttaaagct tcaaaggttt
    11221 gcttaggcag gcggtctgtc tccccaatca tttgaatctt aacattattc gcatgtagtt
    11281 ccgggacata attatcataa aactctactg gcaagttcat gataaacttg acttcctgat
    11341 ctggacgggt ccagttttcc gtagaaaaag catagaccgt aataaccttg acgcccagtt
    11401 tgttggctgc cttggtcacg gtttgcaatg cttccatgcc cgccttatgt ccaaaaactc
    11461 gcggttgcat acgtttttta gcccaacggc cattgccatc catgatgatg ccgatatgag
    11521 caggaacctg tgtcggaacc tctacttcca cagccttatc tttcttaaaa aatccaaaca
    11581 tgatcttatt cctattcaaa aatctatcgt ttcattatac catatttccc cattttcttc
    11641 tatcactaag ctatttattc tcaggcacca agcccatttt tcaaaaaaat aagccgcctg
    11701 attgggcgac tttattttta tagggagatt attatgaaaa agttttagga gtttaagtta
    11761 aggtcttctt aacttatgaa cttagtgtac actccctagc ttaaagtttc cttaagtatt
    11821 tttaaaaatc aaatttttcc atttctcctg ccaatttttc ttggataaac gtgtttgata
    11881 gagttccatt cggtcttcat tttctaagaa atgaggagtt ggacgaactt gaaaattcaa
    11941 aatatcctcc aaaccataag gtacatagag ttcaaaatct aattcttcat tcaagcgcag
    12001 tccaactgcc gtacaccgtt ctggatactt actcatagca tcacgagaac tggtatagga
    12061 agcagtgtga ggactgtgct gatgcatata gacctgattt ttcaattccc actggtactg
    12121 aggaaaatcc tctctcagct ttttctccag taataaggtt tcctcataag aaaaatctgg
    12181 atcaaagaaa atcacatcta tatctgtttc atgatcaaaa ggggatttgt ctgacaaaag
    12241 attccagatg aaatttctga cagaacctgc tgccaaccac gagtctttca aaccaaggtc
    12301 tcggatgatc gtcagaatgg ccatcatatc tggactttct ctaaaagcct ctaagatttc
    12361 ttgcttattt ttcactgtat tcataaccta agtgctcata tgccttagca gtcgccaccc
    12421 gtccagaccg tgtccgcatg ataaaacctt tttgaatcaa gtaaggctca tacatgtctt
    12481 caactgtctc acgctcttcg gcgatattca cagaaagagt tcctagacca acaggtcctc
    12541 cactgtacat ctcaatcatg gtgcgaagga ttttttgatc cacatagtcc aaaccttcat
    12601 ggtcaacatc cagcatagtc aaagccttat cggtaataac atcatcgata accccattcc
    12661 ccattatctg ggcaaaatcg cgcacgcgct tgaggagacg attggcaata cgaggggttc
    12721 cacgactacg tagggccaac tcagatgctg cctcatgggt gatttccatc tcaaaaatat
    12781 ctgccgtccg ctcgacaatt tctgtcaagt cagcatgagc ataatactcc atatgacctg
    12841 taatcccaaa acgtgcccgt agtggatttg agagcatacc agcccgagtc gtcgcaccaa
    12901 tcaaggtaaa aggaggcaac tccaaatgaa cactgcgact gccttcacca gccccaatca
    12961 taatatcgat gtagaagtcc tccatggcac tataaagcac ttcttccact gacatgggta
    13021 agcgatgaat ctcgtcaata aagaggacat ctccaggctc taaatcattc aaaatcgcta
    13081 ccaaatcacc cgctttttcg ataacaggac cagacgtttg cttgagattg actcccagtt
    13141 cattggcaat gacaaaagcc atggttgttt tcccaagccc tggagggcca aataagagca
    13201 catgatccag cgcttcatcc cgcattttag cggcttcgat aaagatctga agttgatcct
    13261 taaccttatc ctgaccaata tattcacgta aatactgagg acggagcgtg cgttctacta
    13321 actcctcatc acccatcatc tcattatcta aaattctact catggctcta ttatatcaaa
    13381 aaaaacaagc cacaaacaaa aaagccacct gattgggtga ctcctaagtt tagcacttat
    13441 gtggtataat attatacggc acttctacac cgcctacgaa aggaggtgag atagcccatg
    13501 atggaattag tactcaaaac tattatcgga ccaattgtgg tcggtgtcgt tcttcgtata
    13561 gtcgataaat ggctaaacaa ggacaaatag tgtcaaaaaa gacctcaagc ttatttggtc
    13621 gtgagcttgg ggtcttttct agcctatgat atagaactag tactcaattc ctttttatta
    13681 tcccatagtt cacgaatttt gtcaaaactt tacattttct tcaaccgctg tacgacaaga
    13741 cggttaagat taagagaacg ttagggattc tatcaatttc atagaaattt tgatttcgta
    13801 aacgaagaga caatcttaca tgtcacttct catttaatac gccactacta gacaagcaaa
    13861 atcattatta cagtagttcc agtccttcaa ttaacagtca cttacaatca aattgagttt
    13921 gaactagctg aagcgaccac agacctattt cttagtcata ttcgctaaaa aaatccccgc
    13981 caaaatctca aaaagtcccc gccaattccc cgaccaaaat ccgaaaaata ccgaaaaata
    14041 tcgaaaaatt atttttagaa tagtcccaaa aatcctgaaa tagagctaaa aaactccacc
    14101 tgattcggtg gagttaaggg agattattat gaaaaagaaa agtttaggat tttattaaat
    14161 aaagttagga ggtctttatt taataactac atgatacaag acgaaactta aaactagctt
    14221 aacttttcta aaattttact attttgcaaa aaatttctat caccagcacc tcac
//
LOCUS       DBDESG010000059        13939 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.059, whole genome shotgun sequence.
ACCESSION   DBDESG010000059 DBDESG010000000
VERSION     DBDESG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.059"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..223
                     /locus_tag="ACKB38_001791"
     CDS             <1..223
                     /locus_tag="ACKB38_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGL7980520.1"
                     /translation="LLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILK
                     LGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(418..1989)
                     /gene="ffh"
                     /locus_tag="ACKB38_001792"
     CDS             complement(418..1989)
                     /gene="ffh"
                     /locus_tag="ACKB38_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGL7980521.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(2001..2333)
                     /locus_tag="ACKB38_001793"
     CDS             complement(2001..2333)
                     /locus_tag="ACKB38_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGL7980522.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(2424..2801)
                     /locus_tag="ACKB38_001794"
     CDS             complement(2424..2801)
                     /locus_tag="ACKB38_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGL7980523.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2873..4177
                     /locus_tag="ACKB38_001795"
     CDS             2873..4177
                     /locus_tag="ACKB38_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGL7980524.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            4190..4996
                     /gene="yidA"
                     /locus_tag="ACKB38_001796"
     CDS             4190..4996
                     /gene="yidA"
                     /locus_tag="ACKB38_001796"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGL7980525.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            5039..6037
                     /locus_tag="ACKB38_001797"
     CDS             5039..6037
                     /locus_tag="ACKB38_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HGL7980526.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(6204..6275)
                     /locus_tag="ACKB38_001798"
     tRNA            complement(6204..6275)
                     /locus_tag="ACKB38_001798"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6240..6242),aa:Arg,seq:cct)
     gene            complement(6318..6665)
                     /gene="rplS"
                     /locus_tag="ACKB38_001799"
     CDS             complement(6318..6665)
                     /gene="rplS"
                     /locus_tag="ACKB38_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGL7980527.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(6784..7113)
                     /gene="crcB"
                     /locus_tag="ACKB38_001800"
     CDS             complement(6784..7113)
                     /gene="crcB"
                     /locus_tag="ACKB38_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGL7980528.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(7107..7481)
                     /gene="crcB"
                     /locus_tag="ACKB38_001801"
     CDS             complement(7107..7481)
                     /gene="crcB"
                     /locus_tag="ACKB38_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGL7980529.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(7478..7744)
                     /locus_tag="ACKB38_001802"
     CDS             complement(7478..7744)
                     /locus_tag="ACKB38_001802"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGL7980530.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(7860..8303)
                     /locus_tag="ACKB38_001803"
     CDS             complement(7860..8303)
                     /locus_tag="ACKB38_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGL7980531.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            8407..9342
                     /locus_tag="ACKB38_001804"
     CDS             8407..9342
                     /locus_tag="ACKB38_001804"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGL7980532.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            9438..9680
                     /locus_tag="ACKB38_001805"
     CDS             9438..9680
                     /locus_tag="ACKB38_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGL7980533.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLT"
     gene            complement(9737..9955)
                     /locus_tag="ACKB38_001806"
     CDS             complement(9737..9955)
                     /locus_tag="ACKB38_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980534.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(9952..10703)
                     /locus_tag="ACKB38_001807"
                     /pseudo
     CDS             complement(9952..10703)
                     /locus_tag="ACKB38_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11040..12386
                     /gene="gdhA"
                     /locus_tag="ACKB38_001808"
     CDS             11040..12386
                     /gene="gdhA"
                     /locus_tag="ACKB38_001808"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGL7980535.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(12892..13062)
                     /locus_tag="ACKB38_001809"
     CDS             complement(12892..13062)
                     /locus_tag="ACKB38_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980536.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(13075..13647)
                     /locus_tag="ACKB38_001810"
                     /pseudo
     CDS             complement(13075..13647)
                     /locus_tag="ACKB38_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(13670..13939)
                     /locus_tag="ACKB38_001811"
                     /pseudo
     CDS             complement(13670..13939)
                     /locus_tag="ACKB38_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4309 a         2952 c         2421 g         4257 t
ORIGIN      
        1 ccttctctat ggggttatcg ccagcaatgg tttgaaagtc ttgattaaag aacgtgttga
       61 tttcgctcaa atgcgaaacc tcatcatcgc aagtgctatg ttggttcttg gacttggagg
      121 agctatcctt aaacttggtc cagttacact ttcaggtact gccctttcag ccatgacagg
      181 aatcatcttg aacttgatct tgccatacga aaataaagac taagagtcta aatacaccta
      241 atccactcag acagctgagt ggatttttcg tataccataa taaaagtgtc ttaacaaaat
      301 tattaaaatc aaaaaacgta taatatcaga tattctaaaa ccttgatact gtacgtttta
      361 tcatagaaat ttttacttta ttttctcatc aaatgagatt tgcatcaatc tcttgtctta
      421 cttgcgtttc ttcttcgctt tcttcatttt gttagccata cgtttcatgg actgtttcat
      481 ggcaaattca ccaattttac ctttcaaacc gccaccaaac atctggctca tatctggcat
      541 tcctgctcct ccgagagctg ataagtcagg cataccgcct tgtcccatca ttccttcaag
      601 ggcagacata tccattcctc ccatatttgg catattttta ggaaggttat ttggattaat
      661 ccccatttgc ttcatcattt tattcatatc cccagacata acaccctgca tgagctgttt
      721 agcctggtta aagtccttga tgaatttatt gacttcgacg aatgtatttc cagaaccagc
      781 agcaatacga cggcgacggc ttggatttaa caaatctggg ttttcacgct cttcaggtgt
      841 catcgaagac acaatggcac gtttacgagc aatctggcgt tcatccacct tcatgttttg
      901 aagggctgga ttgttggcca tacctggaat catcttgagc aagtcttcca tcggccccat
      961 attttgcacc tgatctaatt gatcgatgaa atcattaaaa tcaaaggtgt tttcgcgcat
     1021 cttctcagcc atttcaaggg ctttttgttc atcgtattcc tgagaagctt tctcaatcaa
     1081 agtgagcata tcccccatac caaggatacg gctagacatg cggtctgggt ggaaggtttc
     1141 aatgtccgta atcttttcac ctgtaccagt gaacttgatt ggttttccag taatgtgacg
     1201 aacagacaga gcagcaccac cacgagtatc gccatcaatc ttggtaagga tgaccccagt
     1261 cacttccaac tgagcattaa actcacgcgc aacattggct gcttcctgac caatcatagc
     1321 atcaacgaca agcaagattt catttggttg agccaatgct ttcacatcac gaagctcatt
     1381 catgaggagc tcatcaatct gcaaacgacc cgcagtatca atcaagacat agtcgttatg
     1441 attagtttgg gcttgctcca aaccttgacg tacaatctca acagctggta cttctgttcc
     1501 aagtgcaaag acaggcacat caatctgttg tcccaaggtc ttaagctggt caatggcagc
     1561 tggacgataa atatccgccg caatcatcaa aggacgagca ttttcttctt tcttgagttt
     1621 gttggccaat ttaccagcaa aggttgtttt accagcccct tgtaaaccaa ccatcatgat
     1681 gatggttgga atcttaggtg acttgataat ttctgccgta tcagaaccta aaacggctgt
     1741 caattcctca tcaacgattt taataatctg ttgcgcagga ttaagtgtat caatgacctc
     1801 atgcccgact gcacgctcac gaactttctt gataaagtcc tttacaacag gcaaggcaac
     1861 gtcggcctcg agcaaggcca agcgaatttc tttggttgcc tcttggacat cagattcaga
     1921 gatttttcct tttttacgta gatttttaaa gacgttctgc aaacgttctg ttaaactttc
     1981 aaatgccatt tttcttcctc ttattctcta ttatcaatgc ttgttaaaat ttctatctgc
     2041 tcctgcagaa agtcatcctt gggatagcgc tccaaaatct gatcaaaaat ctgactgcgg
     2101 acaatatagt ccgagtacat gtgcaatttc atctcataat cttccagaat cttttctgtt
     2161 cgcttgatat tgtcatagac agcctgacga ctgacaccga actcctcggc aatttcagca
     2221 aggctgtaat catcagcgta gtagagctcg atataattca tttgcttatc tgtcaaaagc
     2281 gccgcataaa attcaaagag cgcattcata cgattggttt tttcgatttc cataactttt
     2341 attataccaa aaattagcct aatctaccac actaggaagc cgatccaaga agatagatag
     2401 ctaaatttga aaaagacatg agcctagccc caagtaattt ccaattgata gctggcaaag
     2461 ggatgtccct cttgattttg tagttgataa tctagttcaa tcttttgcct atcaacttga
     2521 taatggctcg tttggatgat aaactcctgc atgcccatag gtgtaggaat ataggctaaa
     2581 ctatcgctat cctttagaaa gcgcataatg gtcttgggat tagaaaatcg gctcatcaca
     2641 agttcttgac catgaaattt aatcactact ttttcctttt cctcattata gaaaagcagg
     2701 tagctataat ctcctttttc atgcacttcc acatcataaa gctggtcaat cacttccaac
     2761 tgctcatcaa actgaatcgt atttcgcatc cgaatcttca catcaggccc tctttcttgt
     2821 ctcttgtcct actattttac caaaaagagc aggattttgc tataatggtc atatgaacga
     2881 aaaagtattc cgtgaccctg ttcacaacta catccatgtc aataatcaaa tcatctatga
     2941 cttgattaat acaaaagaat ttcagcgttt gcgccggatc aaacaactgg gaacttccag
     3001 ttataccttc cacggtggag aacacagtcg cttctctcac tgtctaggag tctatgaaat
     3061 tgcacgacgc atcacagaga ttttcgaaga aaaatatcct gaggaatgga atcctgccga
     3121 gtctctcttg accatgaccg ctgctctcct acacgacctt gggcatggtg cctactccca
     3181 tacttttgaa catctctttg atacagacca tgaagccatt actcaggaga ttattcaaaa
     3241 tcctgagaca gagattcacc aagtcctgct acaagtggca cctgatttcc cagaaaaggt
     3301 ggccagtgtc attgaccata cctatcctaa taagcaggtc gtgcagctca tttctagtca
     3361 gattgacgca gatcgcatgg actatctctt gcgcgactcc tattttacag gagcatccta
     3421 tggggaattt gacctgactc gaatcctccg agtcattcgt cctatcgaaa atggtatcgc
     3481 ctttcagcgc aatggcatgc acgccatcga agactacgtc ctcagtcgct accagatgta
     3541 catgcaggtt tatttccacc ccgcaacacg cgccatggaa gttctcctac agaatcttct
     3601 caaacgcgcc aaggaactct atcctgagga caaggatttc tttgcccgaa cttctccaca
     3661 cctcctgcct ttcttcgaaa aaaatgtgac cttgactgac tatctggctc tggatgatgg
     3721 cgtgatgaat acctacttcc agctttggat gaccagtcct gacaagattc ttgcagattt
     3781 atcgcatcgc tttgtcaacc gcaaggtctt taaatccatt accttttcac aagaggacca
     3841 agatcaactt actagcatga gaaaattggt tgaggatatc ggctttgatc ccgactacta
     3901 cactgccatt cataagaact ttgacctccc ttatgatatc tatcgtcccg aatctgaaaa
     3961 cccacggaca cagattgaga ttttacaaaa aaatggagaa ctggccgaac tctctagcct
     4021 gtctcctatc gtccaatccc ttgctggcag tcgccacgga gataatcgct tttattttcc
     4081 aaaagaaatg ttggaccaaa acagcatctt tgcaagcatt acccagcaat ttttacactt
     4141 gattgagaac gatcatttta ccccaaataa aaactagaag aggaaattta tgagtattaa
     4201 actaattgcc gttgatatcg acggaaccct tgtcaacagc caaaaggaaa tcactcctga
     4261 agttttttct gccatccaag atgccaaaga agctggtgtc aaagtcgtga ttgcaactgg
     4321 ccgccctatc gcaggcgttg ccaaacttct agacgacttg cagttgagag acgaggggga
     4381 ctatgtggta accttcaacg gtgcccttgt ccaagaaact gctacaggac atgagattat
     4441 cagcgaatcc ttgacttatg aggattatct agatatggaa ttcctcagtc gcaagctcgg
     4501 tgtccacatg catgccatta ccaaggacgg tatctatact gcaaatcgca atatcggaaa
     4561 atacactgta cacgaatcaa ccctcgtcag catgcctatc ttctaccgta cccctgaaga
     4621 aatggctggc aaagaaattg ttaaatgtat gtttatcgat gaaccagaaa ttctcgatgc
     4681 tgcgattgaa aaaattccag cagaatttta cgagcgctac tccatcaaca aatctgctcc
     4741 tttctacctc gaactcctta aaaagaatgt agacaagggt tcagccatta ctcacttggc
     4801 tgaaaaactc ggattgacca aagatgaaac catggcaatc ggtgatgaag aaaatgaccg
     4861 tgccatgctg gaagtcgttg gaaaccccgt tgtcatggaa aatggaaatc cagaaatcaa
     4921 aaaaatcgcc aaatacatca ccaaaacaaa tgacgaatcc ggcgttgccc atgccatccg
     4981 aacatgggta ctgtaaaagt atcatttttc aataagaatt gattagcaat aaaatccaat
     5041 gaattttttt agcaaactat ttaatttaaa acaaaataat cataatagag acacaaattc
     5101 tgattgtaac aatttttacc taaacgaatt agaatgtggc cttactcctg ggcaactcat
     5161 actcatagat tggactcaaa aaacagggag aaattataat ttcccaagat attttaaata
     5221 ctctcttcaa attgaccctg aatctacaca caatcaatta tacaaattag gatacttcac
     5281 taaaaataag actttatcat atcttacagt agtagaatta aaaactatat tatctaaaca
     5341 taatttagct acttctggaa aaaaagcaga attaattaca agaataatta ataatgttaa
     5401 cattgacaat ttagatattc cgttcgaatt taaactaaca aaagaagcac aaaatcttat
     5461 tatcgaacat agtgactata tcaaagcata ctatgataaa gacataacta tggaagatta
     5521 ttgtaaagaa aaaaacaata tctcttttaa agcaactttt ggtgatataa aatggagtct
     5581 cttaaataaa caagctcata ggaatactgt atcaggagat tttggatgct tatctaacac
     5641 acgaaaggct cagggaagac atttggaaca agaaggtaat attaaacatg ctttaatata
     5701 ttacatagaa tctttgataa ttactatttc aggattagaa aacaattttt cagccactga
     5761 ttatccagta tattatcccg attcgatacc tgactactca ctaaaacata ttcaaacatt
     5821 aatggaatca ttatctgatg acgattatga ttttgctttt gatgaagcat tatttcgctt
     5881 ctcaattttg aatgcaaatc attttttatc taaggaagat attgactatt taagagttaa
     5941 tttacctcgt tccactgctg aagaaataaa caattactta aagaaatatg aatgttatag
     6001 tcctttaaat aatttagaac ttgacgattt tgaataaatt gactatacaa acatttatat
     6061 actcgatata gtctcaattt tatctgatga ttgcccaaat ttttcaataa taaaacgcat
     6121 aatattatgg agacaatccc ctatattatg cgttctttta atatcaaaga ctttttgaca
     6181 aacttctttg atatctaatt acatgccccc tgcaggaatc gaacctgcaa ctactcctta
     6241 ggagggagtt gttatatcca ttgaactaag ggagctagat aaaaactctg ctaaatgagc
     6301 agagtttttt agtcgaatta acgacggatt tctttgatac gagctgcttt accttgaaga
     6361 gcacgcaagt agtacaattt cgcacgacgt actttaccgt aacgaacaac ttcgattttt
     6421 tcaacacgtg gagtgtggat tgggaagata cgctcaacac ctacaccgtt agagatttta
     6481 cgaactgtgt agttttctga gattccagca cctttacgtg cgataacaac accttcaaaa
     6541 atctggatac gttcacggtt accttcgaca actttcgcat gtacacgaac agtgtcacca
     6601 ggacggaatg atgggatatc tgtacgaagt tgaccttcag tcaagctttg gattaatgga
     6661 ttcattttat tctcctatct ttgtcaatct tgaggaatct tcctcagcgg ataaactgta
     6721 tttttgtgcg tccattacac acaagatata gtttaccaaa tttctaaaaa aaagtaaaga
     6781 aatttatagc aaaattccta aaaaaatcgc aaccaaacca cctatgtaag tcaaagtcag
     6841 gtaagaataa aagaccttct tatcacttag cagtctttga agttcgtcat tcaaggtcga
     6901 aaaagttgtt aaaccgccac aaaatcctgt tgctagaata gcatatactt ccttagactc
     6961 tacatgattg tagaatactc caattaaaaa acaacctaaa agattagcta tgagcgttcc
     7021 gagtggcaat ttagaagctt gattatagcg agaaaagaaa taccgtacga gtgctcccaa
     7081 gccacaggcg attgcaagat agacgattac catttcttcc tccccaaata gtaagctaaa
     7141 agcaggcctc caccgatact caaaagcaaa tacagtatca aactaagata acgccctgta
     7201 tcaagcagct tcacagtatc aagcattagg ctggaaaaag ttgttaaacc tccgcaaaaa
     7261 cccgtcccca gtgctaaaat caagccctta ctagtcccct tatagaccag atagcccttg
     7321 acaagataaa tcaagcagaa aattcccaga tagttgacaa agagagtgcc ccaagggaaa
     7381 tctggactgg ctggtaacca ggtggaaact agatatcgga caagtccgcc caacatagca
     7441 gctagaaaaa ttcctagcgg ataaaattgt tcttttttca tttgatattt tgatcctgat
     7501 aatcacgcga acgtttgagt atgtccgaaa aagtcgcgac aatagtctcc tgatagcgct
     7561 tgtcctctac acgactccta accttttcaa aaataacttc ttctctctta gtatctaaaa
     7621 tcggtttacc tgaagctttc ttataagcaa ccaccccctc aactaaatgc attcgttctt
     7681 ctaacagttt gacaatttgg tcgtcgattt gatcgatttc ttgacgaata atatctaaat
     7741 ccatacagtc tcctccttta tttgaattat tgtatcaaaa agccatcaat taggctagta
     7801 aaatcccaac tctcgataag aaaaatgcac tgattgatta catcagcgca cttttatgtt
     7861 tatcctactt tagcagccaa ttcttctgcg aattgttcca agcgttcaat atcttcttcc
     7921 tcggcagaaa gatcaacttt aacacactct gaaccttttt ctgctcctgt tgacacaaag
     7981 acacggtcaa agtcatcaac agccttacag aattcatcat agaaggtatc tcctgagccg
     8041 accactccgt agattttgcc attcaagttg agatctgcta ggtcttcgta gaagtccatc
     8101 atctcatctg gcaattcacc atcaccataa gtataggtcg caacgatagc gatgtctgct
     8161 tccaagaagt ctgaagcgtc aacagttgta cattcatcaa catcaacatc caagcccaag
     8221 tcacgtaatt tgtctgctac aatatctgca atttcttcgg tattaccggt catactggca
     8281 aatacaattt ttgctaatgc catatcgtcc tcctcaattt atctttctcc attatatcac
     8341 atctttttca ttttaaaaat aaaaattctt gtgctacaat gaaatgagaa agaattgagg
     8401 ttatttatgg agatttgcca acaaatttta gaaaaaatta aagaatacga caccattatc
     8461 attcatcgtc atatgaaacc agaccctgat gccttgggaa gtcaggtggg attgaaagcc
     8521 ttgctggaac atcatttccc agaaaaaacc atcaaagccg tcggttttga tgaaccaact
     8581 cttacttgga tggctgagat ggatcttgtt gaagatagag cctaccaagg cgcacttgtc
     8641 atcgtctgtg atacagctaa tactgctcgt atcgatgata agcgctatag tcaaggtgat
     8701 tttctcatta agattgacca ccatccaaat gatgatgtat acggtgacct gtcttgggtc
     8761 gatactagtt caagtagcgc tagcgagatg attaccctat ttgcccaaac aacccaacta
     8821 gccttggcag atcgcgatgc tgagttgctc tttgcaggaa ttgtcggtga tacaggtcgc
     8881 ttcctctacc cttctaccac tgcacggact cttcgcctgg ctgcttattt gagagaacat
     8941 aactttgact ttgcggctct cactcgcaaa atggacacta tgagctacaa aattgctaaa
     9001 ctgcaaggct acatctacga ccatctggaa gtggatgaaa atggtgctgc tcgcgttatc
     9061 ctgagtcaga aaatcttgaa acaatacaat ataaccgatg ctgaaactgc ggccattgta
     9121 ggtgcacctg gacgcattga cagagtgagt ctctggggaa tttttgtcga acaggctgat
     9181 ggccactacc gagttcgctt acgcagtaaa gtccatccta tcaatgaaat tgccaaggag
     9241 catgatggtg gaggccaccc tctagcaagt ggtgctaatt cctatagcct agaagaaaac
     9301 gaaatcatct accaaaagtt aaaaaacttg cttaaaaact gataaaatac ttgccaaact
     9361 tttcagaatc tgatagacta gtatagtaac aatctatggc tcgcaaagag accatggcag
     9421 aaaggaaata ttgcaaaatg aaaaaagata tccatccaga atatcgccca gttgtcttca
     9481 tggacacaac tactggttac caattcctta gcggttcaac aaaacgctct aacgaaacag
     9541 ttgagttcga aggcgaaact tacccattga tccgtgtgga aatttcatca gactcacacc
     9601 cattctacac tggacgtcaa aagttcactc aagcagatgg acgcgtggat cgtttcaaca
     9661 aaaaatacgg tctcacataa tgataagaga acagttttgg ctgttctttt ttgtttcttg
     9721 aaatcaactg ctgttttcat gttccagact catctgtagg ttcgatttcc atgctactag
     9781 gcaggaagga aatagctgtt tcaacacgtc cataatgagc tatactattg tcacgaacca
     9841 cactttcatt gatggtccaa gtggaattca ttttcttaaa agcttctcgg actttttcca
     9901 aatctttgga ggcaatggcc tgctctaagg tttcaaaacg aggacttata ctcatctgct
     9961 ttcaaaaagc attctagtcc atctccgatt accgatggac tttatcacct ccttctccag
    10021 tccttgtatg acatcttgaa gttgattcat gacatcttcc aaagttcgaa aggctttatt
    10081 cttaaatcca cgtttacgaa tctctttcca cacttgttca atgggttcat ctctggtgtg
    10141 tatggaggaa taaaggtaaa atcaatatta gtcggaatat ttaaggtact tgatttatgc
    10201 catatagcat tgtccataac gagtaaaagg ataagcttgt gaaagctctt ctaaaaaggc
    10261 gttcatccac actccttttt ataaacctga aataaggcat caattgtaac aaattctcct
    10321 gcctctgtag ccttcaaatg acgggcaaga aaggctttct cttcctcaac tgtcatatat
    10381 gcatggttac gaccaccacg tgtttcttga aggagagagt cgagtccgaa ctcctcatat
    10441 ttttttacgt ttcgccaaat cgttgtttga ttacagtcta aaagctctat aatctcttta
    10501 taagatttgc ccatcagacg aaatatagta gattgaaact agaatagtac acctctactt
    10561 ctaaaacatt gttagaaatc gatttgtcct gttcttgttt cattttacta tagaacgatt
    10621 tgaaggcgtt tataatattt agctgtacga gagtctttta aaagtgtttt gatggtttgg
    10681 atttcttctt tagttgattt catattacta ttatataatg ctttttgatt ttagtctggt
    10741 ataaatattg ctttcctcca aaatggtcat agttttactg gcaaatctaa catatcacgg
    10801 ataaattaac aagtgatttc tgaattgcta aacattttct tttcttatag catactttaa
    10861 gattttgtct ttgagaaaga tatttccaag aaaaacgttc gtttttttat ctattttcaa
    10921 aaaaaccaaa aaactgaatt gaaagaattt ttagaaaatt tctgtttttt ctttacagtg
    10981 aaaaaaaatt ctgctataat gtcttatgta ataaaatatg acaataaaag gagaacgata
    11041 tgacatctgc taaagaatat atccaaagcg tgtttgaaac tgtgaaagct cgtaacgggc
    11101 acgaggctga attcctccaa gctgttgaag aatttttcaa cactttagaa cctgtatttg
    11161 aaaaacaccc tgagtatatc gaagaaaata tcttggcacg tattactgag cctgagcgcg
    11221 tggtttcttt ccgtgttcct tgggttgacc gtgatggaaa aattcaagta aaccgtggtt
    11281 accgtgttca attcaactca gctgttggac catacaaagg tggacttcgt ttccacccaa
    11341 ctgtaaacca agggatcttg aaattcctcg gatttgaaca aatctttaaa aatgtcttga
    11401 ctggacttcc tatcggtgga ggtaaaggtg gatcagactt cgatcctaaa ggtaaaacag
    11461 atgctgaagt gatgcgcttc tgccaaagct tcatgactga attgcaaaaa cacatcggac
    11521 catcacttga cgtacctgct ggtgatatcg gtgttggtgg acgtgaaatt ggttaccttt
    11581 acggtcaata caaacgtctt aaccaatttg atgctggtgt cttgactggt aaacctcttg
    11641 gatttggtgg tagcttgatt cgtccagaag caactggtta cggtttggtt tactatactg
    11701 aagaaatgct caaagctaac ggtaacagct ttgctggtaa gaaagtcgtt atttcaggtt
    11761 ctggtaacgt tgctcaatat gctcttcaaa aagcaactga actcggtgca actgttattt
    11821 ctgtttctga ctcaaatggt tatgtcatcg atgaaaatgg tatcgacttc gatcttttgg
    11881 ttgatgttaa agaaaaacgt cgtgctcgtt tgactgagta tgcagctgaa aaagcaactg
    11941 caacctacca cgaaggtact gtatggactt acgctggaaa ctatgacatt gctcttccat
    12001 gtgccactca aaacgaaatc aacggtgaag cagctaaacg tttggttgct caaggcgtta
    12061 tctgtgtatc tgaaggtgcc aacatgccga gcgaccttga tgccatcaaa gtttacaaag
    12121 aaaatggtat cttctacgga cctgcaaaag ctgccaacgc tggtggtgta gccgtttcag
    12181 ctcttgaaat gagccaaaac agtcttcgcc tctcatggac tcgtgaagaa gttgatggcc
    12241 gtctcaaaga catcatgaca aacatcttca acacagctaa aacgacttca gaaacatacg
    12301 gtcttgataa agactacctt gcaggagcta acattgctgc ctttgaaaat gtagcaaatg
    12361 ccatgattgc acaaggtatt gtttaagatt tatttactca atcctcaatc actacgattg
    12421 gggattttta gcttgaatta aaaaattgga aaatctaatt aaataaaatg aaatgaaata
    12481 aaaactgaac aaattgattg cggaattcaa accaatttct aacatatttc tagaaattaa
    12541 tttgactttt ctaatcgatt tgttcatatc ttatttcaat ttactatact tacttgaccc
    12601 tttttatact caatgaaaat caaagagcaa actaggaagc gagccgcagg ctgtacttga
    12661 gtacggtaaa gcaaagctga cgtggtttga agagattttc aaagagtatt aaacaaaaag
    12721 aaaaaccgct actcctaaga atagcgattt aatcatgttt ttaagctact actgtagttg
    12781 ctctattatt taagtgttat gaataacttc aatatcttta aagctttttg ttagatttat
    12841 aatttcaact atagccatct ctactcctta gtttacagca aaaaattatg cttaattctt
    12901 attgtttttg taaacaatca gcgaacccaa tactattcca ttcaacaagc ctaaagaaat
    12961 accatattca actggaaaat ctaaaaaaat tgcaaacact attccaatca tcacccctag
    13021 tgaggctgtt gttaaaatta catattcatt ttctttcttc attttcttta cacctcattt
    13081 cctttttgta tataagctgt agtactctcc accaagatcc attaagtact tatgatttcc
    13141 tgactcaaca atcttaccac ctttcattac aaaaataaca tccgcatcct taatcgttga
    13201 caatctatga gctacaatta tttgagtaca gccctgactt tgtatatact ttgttattct
    13261 ttcctcatta atagtgtcta atgcactagt tgcttcatct aaaattacaa tactaggatt
    13321 atttattaat gcacgtgcca gtgctatccg ttgcctttgc ccacctgaaa tatttgaccc
    13381 catctctgag atgatagtat taaatttcat cggcatagcc atgatttcat catagatttg
    13441 aactgcttta caaacttcct ctatcttttg tgaagtaact tcgtgcttta aagttatatt
    13501 atcaagaata cttcggttca ataaaaatga atcctgtgga actactccta aattttgact
    13561 taatattttt ttatctattt gattaatatt tacaccatca aataaaactt ttcctgtatc
    13621 aatcttatat aatcttgatg gtatttttga taatagactt cctgcaaaac tagaatccta
    13681 gttcatgatt gataatacca gcaatcaaat tcattcgtaa tccgaagcgt ttagatgatt
    13741 tcgataggtt gttgaaatca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt
    13801 gcttctctcc ttagatagcg catggttata gactttatct tcaattgtta gcggtttgag
    13861 tttgctggat ttacgtgaag tttgtgcttg aggatatatc ttcatgagcc cttgataacc
    13921 actgtcagcc aagatttta
//
LOCUS       DBDESG010000060        12739 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.060, whole genome shotgun sequence.
ACCESSION   DBDESG010000060 DBDESG010000000
VERSION     DBDESG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.060"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(17..952)
                     /gene="dapA"
                     /locus_tag="ACKB38_001812"
     CDS             complement(17..952)
                     /gene="dapA"
                     /locus_tag="ACKB38_001812"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGL7980537.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(1008..2084)
                     /locus_tag="ACKB38_001813"
     CDS             complement(1008..2084)
                     /locus_tag="ACKB38_001813"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGL7980538.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(2426..2498)
                     /locus_tag="ACKB38_001814"
     tRNA            complement(2426..2498)
                     /locus_tag="ACKB38_001814"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2463..2465),aa:Thr,seq:ggt)
     gene            complement(2598..3107)
                     /locus_tag="ACKB38_001815"
     CDS             complement(2598..3107)
                     /locus_tag="ACKB38_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGL7980539.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(3105..3201)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3230..3661
                     /locus_tag="ACKB38_001816"
     CDS             3230..3661
                     /locus_tag="ACKB38_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGL7980540.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            3743..4120
                     /gene="mscL"
                     /locus_tag="ACKB38_001817"
     CDS             3743..4120
                     /gene="mscL"
                     /locus_tag="ACKB38_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGL7980541.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTDEKKPTELEVLQEIKALLEKK"
     gene            complement(4162..5256)
                     /gene="hemH"
                     /locus_tag="ACKB38_001818"
     CDS             complement(4162..5256)
                     /gene="hemH"
                     /locus_tag="ACKB38_001818"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGL7980542.1"
                     /translation="MKKAILMMTFGSPEEIIFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGSEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            5411..6634
                     /gene="pepT"
                     /locus_tag="ACKB38_001819"
     CDS             5411..6634
                     /gene="pepT"
                     /locus_tag="ACKB38_001819"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGL7980543.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(6670..7323)
                     /locus_tag="ACKB38_001820"
                     /pseudo
     CDS             complement(6670..7323)
                     /locus_tag="ACKB38_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            7321..7485
                     /locus_tag="ACKB38_001821"
                     /pseudo
     CDS             7321..7485
                     /locus_tag="ACKB38_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7756..8767)
                     /locus_tag="ACKB38_001822"
                     /pseudo
     CDS             complement(7756..8767)
                     /locus_tag="ACKB38_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1,RefSeq:WP_007521835.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(8993..12112)
                     /gene="phtE"
                     /locus_tag="ACKB38_001823"
     CDS             complement(8993..12112)
                     /gene="phtE"
                     /locus_tag="ACKB38_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGL7980544.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENLKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(12322..>12739)
                     /locus_tag="ACKB38_001824"
     CDS             complement(12322..>12739)
                     /locus_tag="ACKB38_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGL7980545.1"
                     /translation="KNKVDQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
BASE COUNT         3695 a         2541 c         2221 g         4282 t
ORIGIN      
        1 gattttattg tctttattag taatctggtc ttaagacccc tgttacagtt gccttagttg
       61 cttcgtagtc gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg
      121 gaacaagagg tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa
      181 ctggagcagg acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa
      241 ttgctgcggc tttcttcata tcgctttctg caatcgcagt aaacatctcg tgcatttcat
      301 ccccatttgt atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga
      361 aagcatctcc atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt
      421 aagccatatt agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca
      481 agcgaagcat ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa
      541 taattggtag gtcagaagca tctgcaatag tcttaaagtg ctgatacatc ccttcttgag
      601 aaggtttgtt gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta
      661 cttctttgac aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag
      721 gaacgcgtcc attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg
      781 tcaaagttgg actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca
      841 ataaatgctc aatcaaggct ggaatagcat caaagttaat ggaaccatcc tcatggaagg
      901 gggtaataaa ggctgtaatg attttacatt tttttaaatc ttgataagac acgaaaacac
      961 ctctctattt caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca
     1021 aatttcaatt cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga
     1081 actgagttcc aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct
     1141 ttttctgcat ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca
     1201 ttgatagctt gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag
     1261 gctgcgatag ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca
     1321 gactcagagt gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta
     1381 tcatcttcca taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca
     1441 ttatcagtga aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg
     1501 tcaccacctg aaggcaagat ttccgcatgc aaatcacgtg gtttcacacc atcattcaag
     1561 acttcacgaa gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct
     1621 tgataagttg aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc
     1681 accatcattt gaattgttga acaattaggg caggcaatga ttccgttgtg agcatcaagt
     1741 gcatgagcat tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat
     1801 gtattatcta ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta
     1861 gaactacctg ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct
     1921 tcaattgtaa tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca
     1981 aggtaacgaa ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca
     2041 ccgacagcac ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg
     2101 aattttctaa aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc
     2161 tttttcatca ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat
     2221 cttcatgtga tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc
     2281 tcatgctcaa tagtagtccg aagactacct atcgactcat cgcatattct atattactag
     2341 agatacttga ttttgtcaat aagaaatttt tcagatcgag tataacaaaa aactctcgta
     2401 ttcaagagtt tccctataca tatcatgcta attgccggga ttgaaccggc gacctcatcc
     2461 ttaccatgga tgcgctctgc caactgagct aaatcagctt acttaagaag tatactatat
     2521 tcagaaaaac ttgtcaagtt tccttagaaa ttttccatag gaaaaagcca ggtaaaaact
     2581 acctagctta tcttcttcta tttacttaaa tcaattcgac gaccaatttt accaataata
     2641 atcgctccaa taatcaatga agcaaattca ccgattcctg ttgtaaacca agtaaggaag
     2701 aatggtaatt ctgctacaat atttaactct gcagcgatag taaacatgga aattgaaaag
     2761 aggattgaaa agaagaaatg atcttttcga atcaatccgt taaagaggta atccttgcta
     2821 tattttgcaa aaagccaaac acctaaactg aggaatacta aggttgaacc accaccaaca
     2881 aagacatcta gcagtccgaa gctaaagaaa ttagcaatca tacaaccgat ggtaactccg
     2941 atgatgtact taggattgta aaaagccata aagttcatca tttctgaaat acgaaactga
     3001 taagcaccat agctgatggc atttaggggc ggtgtgacag tcaaaacgac gtagatagca
     3061 gcgacgattg caatatctgc aacatcacga atagttaatt ttttcatctt ttctcctttg
     3121 gcggttatcc gcgtgtaata tgcttggtga aagaagctaa gcaccaaggg ttgattcatt
     3181 caaccttact agtatagcac aatagcctgc tttatgctat acttaaagca tgaaaaaacc
     3241 tattcaaaaa ttttttaaca atgaaatctt agcctatctc ttttttggta tagtgactac
     3301 tcttgttgct atactatcac gattagtcat ttatcagcta agtcatcagg aactccttgc
     3361 tacggccttg gcaaatatta tcggtatcct atttgccttt atcacaaatg atacgattgt
     3421 atttaagcaa aaaaagaaaa atagacttat ccgtttagtg aaattttctc ttgcccgcct
     3481 ttctactttt ctgttagact tgtttttaac ttttctcttt gtaacccaat ttcctcacat
     3541 catagggcaa ttcgtaactg aaaacattga taagataaat gctattgaaa caataattgc
     3601 acaaatattc attattatca tcaattatat cttgagcaag gtctatattt ttagaaaata
     3661 atgctccatt tatgagcctt ttaattgcct tctataatga tttaggatac aatgaaacac
     3721 gaaacattga aaggaaaaca gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa
     3781 tgttattgac cttgctgtcg gtgttgtaat tgcctctgct tttggtgcta tcgttacttc
     3841 acttgtaaac gacattatca ctcctcttat tttaaatccc gctttgaaag ctgctaaagt
     3901 tgaacgtatc gctcaacttt cttggcatgg agtcggctat ggtaacttct taagtgctat
     3961 tatcaatttt atctttgtgg gtaccgccct cttctttatt atcaagggca ttgaaaaagc
     4021 acagaagctg actggcataa aggaagaaaa aactgacgaa aaaaaaccaa ccgaattgga
     4081 agtccttcaa gaaataaaag ctctccttga gaaaaaataa tcgattaaaa ggatctagcg
     4141 caaagctaaa tccttttttc tttactcttt gggtaacttg cctgcttttt caagcatttt
     4201 tttgataaga taaggcatct taacattttc acgacctttt ttctcaatca attttttcac
     4261 aaattctggc atttgtaaat cttgagattc agccaaaatg tttttcgtct cgtctgaagg
     4321 aactaactca tcaaaggtct cttcttctgg gagaaattcc ttaaactctt gattttcatt
     4381 gactctaact gtattgacca aagcatcaat caaacgagaa tctgtatttg gcattggtgg
     4441 acgatggtag tttaccccaa attcctgaca taagtcataa cattccacat cattgtcaaa
     4501 caagacctca atatgctcac tgatgaaact aataggtaca aaaatataat ggtctggatg
     4561 ttctgtctgt tctctgagat actccaaaac atctggttta atccatggaa taccaatatc
     4621 actctcactt tgccaagtgt tggtatattg ttctgaactc aagcccagtt tttcagcgac
     4681 tagcttgcta ttttcgaaaa tttggtctat ataaggatca ccaaagtcca aggcaaaaat
     4741 aggcacacta tgggctgaaa agatgacttt aaagctgtct tgctttactt cttcttttaa
     4801 aatcttagca atttcatctg cccaatagtt taagagcgct tcttcctgat accagtcctt
     4861 aatgaccaaa aattgaattt gtttgctttc taaaaatttt tcatagccca tgacagagta
     4921 gaaagaataa tggggctcca aaatcaagca aatacactgt tcaattccgt ctgcttccat
     4981 ttgaccgatc acatctggaa taaagggact ggaaaactta ttagcaaagt agacactgta
     5041 ttcactcccc agcctagctt ctaccaaggc aacctcttga cgggtaattt tttgcagagg
     5101 cgtgcctcca attcgtacat aattatcata aagagtctga atctcgtggt cttggggtct
     5161 caccccacga cgaatatttg tgaaaaaatc agctacacct tcaaagataa tctcttctgg
     5221 tgaaccgaag gtcatcatta aaattgcttt tttcatgact gcttccttgt gatgttttta
     5281 tcaagtttat tttatcatta attcatcgat aagtaaacgc tagcataggg acattctcaa
     5341 tcttctgtct tttgtttttc tcttctttct atgatacaat ggaaaaaata aattcaaaag
     5401 gagttttttt atgacttatc caaatctctt ggaccgcttc ttaacctatg ttaaggtcaa
     5461 cacgcgctct gatgaacact ctactactac tccaagtaca cagagtcagg ttgacttcgc
     5521 aacaaatgtc ctaattcctg aaatgaaacg tgttggactg caaaatgttt actatctacc
     5581 gaatggtttt gctattggaa ccttgccagc caacgatccg tctttaacac gtaagattgg
     5641 ttttatatcg cacatggata ctgctgattt taatgctgaa ggagtcaatc cacaggtaat
     5701 tgaaaactac gatggtggtg tgattgaact agggaattct ggtttcaaac tcgatccagc
     5761 tgacttcaag agtcttgaaa aatatccagg acaaacgctc atcacaacag atggaacaac
     5821 cttgctaggt gctgatgaca agtcaggaat tgctgaaatt atgacagcca ttgaatatct
     5881 aactgctcat cctgaaatta agcactgtga gattcgtgtt ggttttggtc cagatgaaga
     5941 aatcggtgtt ggtgccaata aatttgatgc agaagatttt gatgtggatt ttgcctacac
     6001 tgttgatggt ggtccactag gtgaacttca gtacgagact ttctcagccg ctggtgctga
     6061 attgcatttc caaggtcgta atgtccaccc tggtactgcc aaagggcaga tggtcaatgc
     6121 ccttcagcta gcaattgatt ttcataatca acttccagaa aatgaccgac ctgagttaac
     6181 tgaaggttac caaggttttt accatctaat ggatgtgaca ggtagtgttg aggaggcgcg
     6241 tgcaagctac atcattcgtg attttgaaaa agatgccttt gaagcgcgta aagcatccat
     6301 gcaatctatc gctgataaga tgaatgaaga acttgggagc gaccgtgtca ctctcaactt
     6361 gacagaccag tactacaata tgaaagaagt cattgaaaaa gatatgactc caattaccat
     6421 tgctaaagcc gttatggaag atctaggtat cacgcctatt atcgaaccaa tccggggtgg
     6481 aacagacggc tctaagattt cctttatggg aatcccaact ccgaatatct ttgcaggtgg
     6541 cgaaaatatg cacggacgtt ttgaatacgt tagccttcag actatggaac gtgcagttga
     6601 taccatcatt ggcatcgtag cttataaagg ctaaaaagac gaggtagctc agctacttcg
     6661 cctttctttt tattctactg gtttttcttg atttccagta gttgtagaag attctgttgt
     6721 ttcattttct gaagttgatt cagcaggttt agaatctctt gtattgcttg gtttgttttc
     6781 gtcgctagca gtttcaatgt tagattctgc agttgcgttt ggttggttct cagcactggt
     6841 gttatcacca tttgcttcag catttcttgc tggacttgtt tcttcacttg cgctagcttt
     6901 tgactggatt tgatgattca aaactagaat agcttttgtc gattcaagta aagctgtttt
     6961 gtctttactc ttagcagaaa gttgatctaa taatgcatcc accttatcaa agtccgcatc
     7021 agatccatta ttactttcta aataagagtg aagcgacatg agaatatcgt agagtttttg
     7081 atagagtaca agtgtctgag gatcttgctc agcattttcc ttttcttgtt gaagggcgct
     7141 agcgatacga gtcaagacat cttttacctg actgtttact tcatccaagt ctgcatcagc
     7201 cttgtttgtg gcagctttta gattttctac ttcttctgcc aaggattgtc tgattccttc
     7261 ttcatggatt tgttccaaga gttgatttgc cttgctcaaa agactttcta cttcttcctt
     7321 gctatctgtc gcagattatt ggttgctatc taccatgtac tcctaaaaca ggagagttat
     7381 aatccaagat tacaaggcct tacagaaata agaaatccag ataagacaat gttcgtccaa
     7441 gacgctattc gcttcgcaca gcagcacgga ttcaatatgc tttaatttta aagtttaggt
     7501 gtcaagacct ctttttagtg tgcccaaaat ttagagaagt aatcaatcaa ctaactttta
     7561 tttttttcaa actttcagta aactgaccta aagctaactc aatctgtctt tgtagatgct
     7621 tctgctatca gctagaagtt gatctacttt tgccaagact gccttctcat caaaagttcc
     7681 aggttgatag ttggattgca gggatggaat cttgtttttc aaagccgctt catatccctt
     7741 agtttgaacc ttgatgtagt gattgtggtc gccatgagga atcacaaaac cttctgaatc
     7801 ttcacttata attcgattgg catcaaaacc atgaccatct tcttcctcat gatggacatg
     7861 tagtgacgga ttacttaata cagaactaga agaacttcct acctcttccg tgttagagtg
     7921 tgatggggga ttgttaagag atgacttagg aatatagtga tagtgatccc catgtcttac
     7981 tatataagca tcacctgtat ctctgacaat atcattaggg ttaaagacat atgtggctgc
     8041 taattcacct gccgacaagt cactctcagg aatgaaatga tagtgaccac catgtggtac
     8101 tatagtagat tgaaatagaa tatgagcaaa ttgataaggg gattttaaag taatttctaa
     8161 caatgattta gaaactatga tgtgctattc taaattcaac tcactatata taaccatcat
     8221 cggtagtata acgtccctgt aattttgcta cagatacttc tgcactagct cctttatcgt
     8281 ctttaccatg ttcttgtttt tggcgattga tttcatcttt tgttcgtaca ttttctgcat
     8341 gagcttgatc tttaaggtaa acataatact ttccatctac cttaataata tatcctccct
     8401 taacctaact gacgatatct tgatctttcg gctgatagtt gggggctttc attaatagct
     8461 cttcactaaa gagcgcatca aaaggaactt taccattata gtagtgataa tgatcgccat
     8521 gagaagttac ataaccttga tctgtaatct taataacaat ttgttttgct tgaattcctt
     8581 ctttttgact aacctagtct ggagtcaaat tttcagtctt cttagtgtct ttattactgt
     8641 ttacatatga aacacgattt ttatctgtat tggcctgtta gctatgttgg ttcagagcat
     8701 aaacacacag acttaaggaa aggataacaa cagatccagc tgctatatat ttctttttaa
     8761 atttcataat tacctcattt ctataattat ttatatgatg tcttcattat taaatgatta
     8821 aataaattaa ttaaccaatt aattaactag taaatattcc acctcttttt aagttgtatg
     8881 tcaagaaatt ttatatatta ataataaaat gaaattctcc caaagtcaga gttttatttc
     8941 taacttttga gagaacttca tttttgattc agactttttc tactgctatt ccttacgcta
     9001 tgagatcaga taaattcttt tttatcactt ctccacttgg caatcttaat tcaatcgttc
     9061 catccatatt gaatataaca ctatctaagc ctaatccgta actagctgta aatttttcta
     9121 atttttcttg tacaggatct actgctggag cttcctctaa tgctggatct aacatagggt
     9181 cactccccac attcccttct ggattcaaca ttccattatc cgttgagttt tctggtttta
     9241 caggtttttc gtttggtgcc tctggtaaag aatctgctgg tttattttct gttggttggt
     9301 tctcaactgt tccagtagat acttttccat tttcagatgg tttattttca ccatttcctt
     9361 gaggtgcttc tcctgtaaaa tctgccatat tctttttaat gacttctccc gatggtaaat
     9421 ataattcaat tgttccgtcc atattaaaca agacattttc tagcttcatc ccataacttt
     9481 cagcaaattt tgctactttt tcttgtacag gatccactgt aggaacttct tctaacgttg
     9541 aattactagt actattccca gtttcagaaa gtttttcttt ttctaccttc tcactagtct
     9601 ttggttcttc taccttttca tcaagtttta agttttcttg tgctttattc cttttaaatt
     9661 gtggtagaat acttggttta tcagtttgat tttctttttc caagataggt acttccacaa
     9721 tataagtcga ttgattgtcc aaataagcat ttgccatgaa ggttacagga attttatttc
     9781 cggccgttct ggttgttcct tggtttaatt tcggaatcgg taatttgatt tcaccaactt
     9841 tatagttatt ttctaaataa gcatttccat gaaattcatc aaacactctg actaaagcat
     9901 cagttccttt aggcactgca aattgagggt tcactcttaa ataagtatcc cctgcatgga
     9961 aaggatagaa aatcgtttga ctggccattt tgtaagctaa agaggttgga actgtaaatg
    10021 taccatcata acttacttct ggataatctt ttgaagcgat agtatactta aatgtttgtc
    10081 ctggtaaata aggttgatct aattcaaagt ttgcaatatt ccctactcct tctccaaata
    10141 ctttaccaga tactttctcc aatacttttc catctggtgt tattaatttt actagcatat
    10201 tgatacctaa ttttttctcc aattcaggcg gaaaactaaa agaaacgcgt ttttgaccat
    10261 tggctagagt aaagttttga ttattaaacg tactattttt taacaaatta acaacattcg
    10321 ttaattcttc tccagtataa actttattcc cttctttttt agcaactcct tcttcgggtt
    10381 taaacagttc atagttactg tgagaatgac caattccaac cggtttatgt tcatcaatcg
    10441 gatctgcatg atggtgatct ccatgcggat aaataatcgc atttttttct ttattcacga
    10501 caatactttc acgtttgaca ccatattgtt tcataatgcc agcaattttt tcttcgattt
    10561 ttttatctaa atctttcatt tctttggcat tacttggata atcctgttca tgagatgaca
    10621 aagaatctaa tccattatga ctagttttaa cttcctctaa atgtttttgc gcagccttaa
    10681 tttgctcttc tgtcaagtcc ttcttgaaga aataatgatt gtggtctccg tgactcatga
    10741 caaaacctga ttcatcttca gcgataatac gattagcatc aaatccgtat ccatcttctt
    10801 catgtttctc atgtgaagtt cctggattga ttggaagaga tggagaaggt gttgctagac
    10861 tattgtttgg aagagtcggt tgcccaattt gatttgattt tggaatgtaa tggaaatgat
    10921 caccatgtct tacaatataa gctgtagccg tttcttcaac gatatctttt ggattaaaaa
    10981 tataaccatc agatgctgaa gagagctcct tacttgtcgt taaagaagaa ggattgcttg
    11041 aaagactgcc tagactagac actacttcat taggttttgc atttgtagaa actgtagaac
    11101 cagttccact gataggcacc attctggcaa tcttttcttc taaggcagaa agcttgctgt
    11161 aaggaataaa gtggtaatgg tcgccatgcg gaatcgcaac tccatttggt gtacgactga
    11221 taatcttagc agggtcaaag accaggccat ctgattcact gtaacgttgg gcgctaggtg
    11281 aatcatagag ttccttcaaa agactctgga gattttcaga tttatttgct ggcttgctag
    11341 ttgatccttt tgctacagat tgcgtgttat tgtcactagc tgttgaagaa tagcttaact
    11401 gactcggttg catatttttt ccagccagat gtgctttagc tgctgctaat tcactagcag
    11461 ataaatcgct tttgggaatg tagtgatagt gacctccatg aggaacgata taagcattac
    11521 ccgtatcttc gataatatca gctggattaa agacataacc atcatttgtc gtatatcgtc
    11581 cctgagacct tgctacagca acattagagt taaccttctc attatctttg acatgttctt
    11641 gtttttgacg attgatttca tctttagttc gaacattatc agcatgagct gcatctttca
    11701 ggtagacata atattttcca tcgaccttga tgatataacc acccttgact tcattgacaa
    11761 tatcagcgtc tttaagttga tagtttggat ccttcatcaa gagttcttca ctaaagaggg
    11821 catcataagg aactttccca ttatagtaat gatagtggtc accgtgtgac gttacatagc
    11881 cctgatctgt aattttgatt acaatttgct cagcctgaat tccttctttc tggctaacct
    11941 ggtctggtgt caagttttca cttttctgac ttgactggct gccatccaca taagagacac
    12001 gattattgtc cttattttcc tgcgaacgat gctggtttag tgcataggca catagactca
    12061 aggatacgat aacagctgat ccagctgcta tatatttttt actaaatttc ataaatccct
    12121 catttcaata aatgatgaag ttttttctca acttctttta ctttattaaa tagttttcta
    12181 aaccggttat ttaaccattt aattaaccag taaatagttt actatttaaa attacttttg
    12241 tcaagtttct gttacgtttt tctaaaaaag agaagtaaca cgaaaggtta cttctccata
    12301 aaatatgctc cattcatttt actactgtat aggagccggt tgactttctt ttaacaaagc
    12361 caagagacta tctacttctg ctgaaatagt gttattatct ttcgttccga gaagaagact
    12421 actttttaga ccagtcaatg tctccatagc attttgtcta atactaggat ctgttacttt
    12481 ttctagcaag gcctccgcat ctgctatctt agcgttaata acagaattct ctacttgagg
    12541 aatttcagcc tcatctgtgg tatcttcagc ttcttcctct gtctcttccg tatcagtgct
    12601 tggtttataa agattatctg ctgaaggatt taaaccagcg tgattctctt tttcatcaga
    12661 ttcagggtga gttggctcac ttacttcatc atgttcctta tcttcatcag gtttactgtc
    12721 ttggtctacc ttattttta
//
LOCUS       DBDESG010000061        12151 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.061, whole genome shotgun sequence.
ACCESSION   DBDESG010000061 DBDESG010000000
VERSION     DBDESG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.061"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..126)
                     /locus_tag="ACKB38_001825"
                     /pseudo
     CDS             complement(1..126)
                     /locus_tag="ACKB38_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(229..366)
                     /locus_tag="ACKB38_001826"
                     /pseudo
     CDS             complement(229..366)
                     /locus_tag="ACKB38_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(750..1946)
                     /gene="tuf"
                     /locus_tag="ACKB38_001827"
     CDS             complement(750..1946)
                     /gene="tuf"
                     /locus_tag="ACKB38_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGL7980546.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2868..3737)
                     /gene="gla"
                     /locus_tag="ACKB38_001828"
     CDS             complement(2868..3737)
                     /gene="gla"
                     /locus_tag="ACKB38_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGL7980547.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3961..4569)
                     /locus_tag="ACKB38_001829"
     CDS             complement(3961..4569)
                     /locus_tag="ACKB38_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGL7980548.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4527..5633)
                     /locus_tag="ACKB38_001830"
     CDS             complement(4527..5633)
                     /locus_tag="ACKB38_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGL7980549.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5744..5998)
                     /locus_tag="ACKB38_001831"
     CDS             complement(5744..5998)
                     /locus_tag="ACKB38_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980550.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5992..6234)
                     /locus_tag="ACKB38_001832"
     CDS             complement(5992..6234)
                     /locus_tag="ACKB38_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980551.1"
                     /translation="MYQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(6356..7200)
                     /locus_tag="ACKB38_001833"
                     /pseudo
     CDS             complement(6356..7200)
                     /locus_tag="ACKB38_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            7434..9152
                     /locus_tag="ACKB38_001834"
     CDS             7434..9152
                     /locus_tag="ACKB38_001834"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGL7980552.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            9263..9610
                     /locus_tag="ACKB38_001835"
     CDS             9263..9610
                     /locus_tag="ACKB38_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGL7980553.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9889..10725)
                     /locus_tag="ACKB38_001836"
     CDS             complement(9889..10725)
                     /locus_tag="ACKB38_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7980554.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10738..11367)
                     /locus_tag="ACKB38_001837"
     CDS             complement(10738..11367)
                     /locus_tag="ACKB38_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGL7980555.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11377..12018)
                     /locus_tag="ACKB38_001838"
     CDS             complement(11377..12018)
                     /locus_tag="ACKB38_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGL7980556.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
BASE COUNT         3557 a         2524 c         2206 g         3864 t
ORIGIN      
        1 ctttctcttg ctcaaccgtc atataagcat ggttacgacc accacgtgtt tcttgaagga
       61 gagagtcgag tccgaactcc tcatattttt tacatttcgc caaatcgttg tttgattaca
      121 gtctaaagtg tattgaatct ataacagtac accttggctg ctaaaatatt tctataaatt
      181 agtttgactt tcctgataga gttgttcaca tcttatttca aaccactata aaagttctat
      241 aatctcttta taagatttgc ccatcagacg aaatagaacg atttgaaggc gtttatgata
      301 tttagctgta cgagagtctt ttaaaagtgt tttgatggtt tggatttctt ctttaattga
      361 tttcatatta ctattgtata atgctttttg attttagtct agtattatac tcttcgaaaa
      421 tctcttcaaa ccgcgtcagc ttcgccttgt cgtatagatg ttactgactt cgtcagttct
      481 atctacaacc taaaaacaat gttttgagca gtctgtagct aactttctat agtaaaatga
      541 aataagaaca agacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag
      601 tagatgtgta ctattctagt ttcaatatat tatagtttgc tctttgattt tcattgagta
      661 ttagttcaat atctaaaaaa agaaccttgc caagcaagat tcttttagtg tctaacttaa
      721 ataattgttc ttctgggaac taaatcgaat taagcttcga tttctgtaac catacctgaa
      781 ccaacagtac gtccaccctc acggatagag aatgtagtac cttgttctac ggcgattggg
      841 tgaatcaact caacgtcgat tgtcacgtta tcaccaggca ttaccatttc agtacctgct
      901 ggaagttcga ttgaacctgt aacgtcagta gtacggaagt agaattgtgg acggtagttg
      961 ttgaagaatg gagtgtgacg tccaccttct tctttagtaa ggatgtagac ttcacctttg
     1021 aatttagtgt gtgggttgat tgaacctggt ttagcgataa cttgtccacg ttcgatttca
     1081 tcacgttgaa caccacgaag aaggacacct acgttatctc cagcaagacc ttcgtcaagt
     1141 tgtttacgga acatttcaac accagtaaca actgcttttt gagtttcttc tttgatacca
     1201 acgatttcga tttcgtcgtt gactttaacg ataccacggt cgatacgtcc tgaagcaact
     1261 gtaccacgtc cagtgattga gaatacgtcc tcgactggaa gaagcaatgg tttgtcagtg
     1321 tcacgttctg gttctgggat atactcatca actgtgttca tcaattccat aacgatgtct
     1381 tcgtatttag agtcaccttc aagagcttta agtgctgaac cttggataac tggaagatcg
     1441 tcacctggga agtcgtattc tgacaatagg tcacggattt ccatttcaac caattcaagc
     1501 aattcttcgt cgtcaaccaa gtcaactttg ttcatgaaga cgataaggtg tttaacacca
     1561 acctgacgtg aaagaaggat gtgctcacga gtttgtggca ttggtccgtc agttgaagct
     1621 actacaagga tagctccgtc catttgagca gcaccagtga tcatgttttt aacgtagtcc
     1681 gcgtgtcctg gagcgtcgat gtgagcgtag tgacgttttt cagtttcgta ctcaacgtgc
     1741 gcagtgttga tagtgatacc gcgttcgcgt tcttctggag cagcatcgat agacgcatag
     1801 tctttaggtt ggttaactga tgaaggcaag cgacgtgcca aaacagttgt gatagctgca
     1861 gttagggtag ttttaccgtg gtcaacgtgt ccgatagtac caatgttaac gtgtggttta
     1921 ctacgatcgt atttttcttt tgccatttga gtaaaagcct ccaataaaat atattttata
     1981 gatagacagt aggcaataca gtctaacttt ccttactatt ttatcaaatt tcagctaaat
     2041 tgcaagtgtt ttacaacgtt tttgtgaatt attcttggta aaaatttaac taattaatag
     2101 aaagtactaa atagacttat ccttcaagga gttttcagca ttatccatcc actcacttgc
     2161 tgaaaatgta aacagtctct ggtttatata gtaaaatgaa acaagaatag ggcaaatcgt
     2221 tcaggacagt caaatcgatt tctaacaatg ttttagaagt agatgtgtac tattctagtt
     2281 tcaatctact atacaacctc aaaactgtgt tttgaacaac ctgcggctag cttcttagtt
     2341 tgccctttga ttttcattga gtataaaatt gcagataaaa cgataacaat cataggactc
     2401 agcagagtca atgtaggtcg aagaatagcc cccctaatcg cttattttat agtaaaatga
     2461 aatagaaact gaacattttg ttgaggaagg taaatcaatt cctcacaatg tttgagaaag
     2521 aagggaggac tattccaact tcaacccact ataaagcaga cttacaagat agggaaccca
     2581 tctccgataa tattccgata atatatagta gaatgaaaac tgcacaattt gattggagaa
     2641 ttcaaaccaa tttctcacaa tgttttagaa acaagggtct attatttcaa ctgcaaccta
     2701 ctatagtcca ttgaagctgg aatagcccac tgagacttct aaaccactgc tggagaggag
     2761 tttgtttaga gtttctttca tttccctaaa aaatcaaaaa gagagaacca gtctgatcct
     2821 ctcttactta tagatggtgc tcactcaaat gttaaaggag ctatttctta gagataaagg
     2881 aatttgaata cagctactgc cgcaattgct gctgcgatag gtgctactac tggtacccaa
     2941 gaataccacc attttgaatc gcctttatgc tcaccaagaa ctgatttggg aaggaaagca
     3001 tgaaggagac gtggtcccaa gtcacgggct gggttcaagg caggtcctgt aggtcctcca
     3061 agtgatgtta ccaaagccat aacgaggaat ccaagtgcca agtgagccac agaaagtcct
     3121 gaagcagtgt gtggagccac ctgtgcttta atagccaagt cagaaaaatc aactgtttgt
     3181 cctgcttctg ttgccttttg tttcatgaat tgaagcactt cagcaccaaa gaagttttta
     3241 gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa caaactcatt aatcaaacca
     3301 ttgacagttg ctgcatagcg actttctttt gtaccatggt caatacttga aatagttgag
     3361 aaagttccca agatgttatt tgggttttca gttttcaagt agaatggacg gtatgttgcc
     3421 acaactaagg cttggccaaa gatagccccc aagacttgcg cgataatgta aggtaccact
     3481 tgtgcccaag ggaaaagacc gctaactgca agccctagag tgaaagcagg gttgatgtga
     3541 ttcccagata cgttaccaaa catcaaggct gggatcataa cccccatacc ataaccaaca
     3601 gcgatgacga tccagccact ttggtgacct ttcgtacctt taagttcaac gttggcaact
     3661 gcaccattcc caagaatgat caaaatggca gttcccaaaa attcagtggc atacttcagt
     3721 gcccatgtga aatccatttg ctagattctc cttaaaattt tttagatatt ccttatttta
     3781 tcaattttta ccctatttag caactgattt ctggaaatat ttttaaaaaa ataggattca
     3841 tagtttccca gttcaacaga cttgtaagcg tttttattct ttacgaaaga cctcgttctc
     3901 aaaaccagga aaaaagccca catctgtgag cttttcctgg ttcctagatt ctaaaatcta
     3961 ttatttttca tcttcttttt tcttgaaaaa tcctaatcct agaccagcta acaaggtcat
     4021 gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca attcctctct
     4081 cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt cagtttttgg
     4141 agtacctggt tctggagttg gtttatctgg tgttggagtc tcttgatttg gtagttctgg
     4201 gtttggtgtc ggaacacttg gatgctctgg atttggttgt gggattggtt tttctggtgt
     4261 tgataaacgg tcatacctta ccgttatttc tttatcacta gagtctgacg taacttcttg
     4321 tgattcaact gttggaatat ctggatcttt gtacttgtca atcttaccag atataacctc
     4381 gtcccagttt cctgttgtcc attcaccgta ggttacaact cccgtgacct tgttctcagt
     4441 ttttgtacgg cttaaggtta caggttgaac aacatcttct tttacatttt ggttcgtaac
     4501 tttatcaacg taatgaatga tacgcgttat agtcttcgtc tcagtagagg ttgctgtttt
     4561 gggaaccact gtttcctcaa cattctcacg gtagtaatag tcaactgttg caccgtcttc
     4621 tggtacgcat ttgcaggagt tgctaccaag gtgtatgttg ttttccttgt gataactcgg
     4681 tcttctttgt cctcagttgt tgttttccct tcaatagttt ttgattctgt ggtatactca
     4741 gaacctatcg ctaaatcagc ttttataaca gactctacca acttctcttg cctaccttct
     4801 ttatagtaat tggctgttac tgtagcagtg gttggcgctt cgctttctct ataaactaag
     4861 gtcactgttc taccttcgct tacaatattc ccagttaaac ttgcagaatt tgtatctgct
     4921 tctttaaaag tataatattt tccgtcagta gtagtcatgc tactgagttt tttatctgtg
     4981 acattatagc tggtaccaat cagttgtttt ttattggtaa tgtaggttcc gtcactttct
     5041 ttttctccaa ttccagtatc atttttcaat gatagcaaac gcccttgttc atcaacatag
     5101 cgaactttca cattttctga gattagttct gccaattctg aggttttttt ctttttcttg
     5161 atttcttcgg ttattttccc tttctcttct tcgggaatat ttagttttgg aatgattttt
     5221 tcaacaacgg ttcgtgatgg ttccacagta tcttggatga ctgaaaagtc agctagaatt
     5281 gggagattat actgaacacg gtgactttga gtgtttactc ctactctttc attattctct
     5341 gaaaatactt gtacggtata agaaacaaca tcttttccta atagaacatc cccagtagag
     5401 aaatagccgc cttttcctag tttgctatct ccagagccca cttctttcct aatcttatca
     5461 gatagttttt taccagtcag tacattcgtt cgcacaatcc ctttgtctac ccctacaaag
     5521 tgggagaact ttttgaactc ttcagaacca gatctagccc aaccattatt aagggcattt
     5581 gcttttgtat ttgtattctc tctcaaaagt ttggcgatta gaattatatt catcggcact
     5641 tagagttgct gctatatctg actcttgaat accaacttcc ttactaccat ttctagcggc
     5701 agtatatgtg tatttatctg tttctacttc tagctgatta tccttaacat gataggtaat
     5761 tttctcctgg tataagagtc ttcagtagtt tatatactct gtcatcatct ttagctttag
     5821 aagcagctga gtttagccaa gttggggtat taggagtatc tttatcaacc tgtttcaaga
     5881 cattattcaa gtcatttgct agcctattgg ttatcctcac tgtgaaagtt ttcttccagt
     5941 ctgaaccact tttattagac ttagatatgc tcatgaaatc ctggtcaaca ttcaacacaa
     6001 tatcgccttg ttttttagca tcttccgttg cttctgcttc aaattgatca tggagttttt
     6061 gttgctctgg tgtatagttt ttttatttac acgatttaat gtactatttt ttattttagt
     6121 attaagaact ccattaatac gatttttaaa ctctgcttca aaaaatccct taatttgttg
     6181 gtgcgcaagt tcaacattca aacctttctt atacaagtct tcattctgat acaagtcttc
     6241 attctgaacg atttcggcta cacggtgaat ggtttgattg tataccgtct tctcttaacc
     6301 agcagtatcc ttagttttaa tgactactcg ataggttcct ttagaaccgt tatagtcatc
     6361 tagttttaaa aaagcattcc aaacattcaa aaatagttgg aacttccctt aatacatccg
     6421 tcactttctt tttcaagata gcccaagctt gctcaatagg attcaaatcc ggtgaataag
     6481 gtggtagagg taagaaaaag tgtttatctt ggatgcagag ttcatccaaa atgttcttgg
     6541 gatgaaaacc agcattgccc atgacaataa catgaggtgt cttcaaagca ggtaggagtt
     6601 gcgttttgaa ccactccaca aagaaatcgc ttgtcatgct tttcttgtaa atcatgggag
     6661 ctataaactc tccgtctact tgtcctgcaa caattgaagt tcgctcaaaa cgacgtccgc
     6721 taatcttttc atagactttc tcccctctag gagcccctgc ataaggacga tagagatagc
     6781 ggtcgattcc tgtttcatca atataaacag gtgctaggtg ctttaaacta ttaaaattct
     6841 taagaaataa ggctactttt ctgggtcttg ttccttaaag ctcgtcatct ttttttaaag
     6901 tgaccttaat ctgctttaaa gctgcccata ctgaaggaac agcacaatca aaatgtgccg
     6961 caatttcccg taaaaaagca tctggatgag cctctacaaa ggctttcaat tcctctaaag
     7021 gaatctttcg gtttttgacg actcgctttt tcctctctat gtgttcttgt tcacgtcttt
     7081 cttttcccac gtgaagagag ttctgacgcc aacaccaaaa aacttggctg cctcgacatg
     7141 gctgtgcccc tctttgatgt aatctaatgc tcgctgttta aaatcagtac tatatgccat
     7201 agctttatta taatatgctt gtttaaaact aagtaactat atcaatgttt tgttatgaga
     7261 agacttactt tttttactga aattgaattt ttcctagcct caacatcata taaccccaaa
     7321 attcaatctg acctggtttg aagagatgtt cgaagcagat aaggttttac taaaactaaa
     7381 aaaaatgata taattaaact gattaaatgt tttaaaaata aaggagaatt tgaatgtctt
     7441 accaagaaaa ttaccagaaa tgggttgatt ttgtggagct tcctgactac cttcgtcaag
     7501 atttggaaaa tatggacgaa aaaactaagg aagatgcctt ctatacaaat cttgaatttg
     7561 gtactgcagg tatgcgtggc ttggttggtg ctggtacaaa ccgtatcaac atctacgttg
     7621 ttcgccaagc tactgaagga ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag
     7681 aacgcggtgt agcaattgcc tacgatagcc gtcacttctc acctgagttt gcctttgaat
     7741 ctgcggcagt tcttgctaaa cacggcatca aatcttacgt atttgaaagc cttcgtccga
     7801 ctccagaact atcatttgca gttcgtcatc tcaactgttt cgcaggtatc atggtcacag
     7861 ccagccacaa ccctgctcca tttaacggtt acaaggttta cggtgaagac ggtggacaaa
     7921 tgcctccaca cgatgcggac gctttgacta cttatatccg tgcaatcgaa aacccatttg
     7981 cagttgaagt tgctgatgtg gaaactgaaa aagcttctgg cttgattgaa gttatcggcg
     8041 aagctgttga catagaatac cttaaagagg ttaaggacat aaacatcaac ccagccttga
     8101 ttgaagaatt tggtaaagac atgaagattg tctacacacc acttcatggt actggtgaaa
     8161 tgttggctcg tcgtgctctt gcccaagcag gatttgactc tgttcaagtt gttgaagcgc
     8221 aagcaactgc tgacccagac ttttcaactg taacatctcc aaacccagaa agccaagcag
     8281 cctttgccct tgctgaagaa cttggtcgtc aagttggtgc agatgttctt gtcgcaactg
     8341 acccagacgc tgaccgtgtt ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc
     8401 tttcaggtaa ccaaatcggt gctatcatgg ctaaatacat cttggaagct cacaaaaacg
     8461 ctggaactct tcctgaaaat gccgctctct gcaaatctat cgtttcaact gacttggtaa
     8521 cgaagattgc tgaaagctac ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta
     8581 tcgctgaaaa aatccaagaa ttcgaagaaa aacacaacca cacttacatg atgggatttg
     8641 aagaaagctt cggttacttg attaaaccat ttgtacgtga taaagatgcc atccaagccg
     8701 ttcttgtagt tgctgaactt gctgcctact accgttctcg tggtttgaca cttgctgacg
     8761 gtatcgaaga aatctataaa gaatatggct actacgcaga aaaaacaatc tctgttactc
     8821 tttcaggtgt cgatggtgct gaacaaatca aagcgattat ggctaaattc cgcaacaatg
     8881 ctccaacaga atggaacgca acagctatca ctgtcgtaga agacttcaag gcacaaactg
     8941 ctactgttgc tgacggtact gttacaaact tgacaactcc tccaagtgat gtgttgaaat
     9001 acacacttgc tgacggttca tggattgccg ttcgcccttc aggtacagaa ccaaaaatca
     9061 agttctacat tgcagttgta ggggaaacca acgaagaatc acaagctaag attgctaaca
     9121 tcgaagcaga aatcaatgca tttgtaaaat aaagacctat aaaagatgag accaaccaac
     9181 ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac tttatggcat actagactta
     9241 tcaatgaaag cgaggtaatc tcatggaaca atttttagat aacatcaaag accttgaagt
     9301 cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa ccttctttat
     9361 cggtcgcaaa acttgccctt actgccgtaa atttgcaggt acattgtcag gtgtcgtagc
     9421 tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa ccaagccaac tcaatgattt
     9481 gcaagcattc cgctcacgct atggaatccc aactgtacca ggttttgttc atattacaga
     9541 tggacaaatc aacgtccgtt gcgactcttc aatgtcagca caagaaatca aagatttcgc
     9601 aggattgtaa aaaatcagct aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc
     9661 aaaaaccttg atattatgcg ttttattgtt cctaaaacag ttacatcagt atttcattac
     9721 agaacatttt aattttggaa gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg
     9781 gctattccat attcaattat ccatattcaa tcgctattat cgttttccta gattacaaaa
     9841 aaaactacgt tttgctaatc aaaacgcagt ttcagccact attttatctt actgtccttc
     9901 ttttacttct ttggttgcta catcttctcc aaaccatttt tggctgattt cttggaactt
     9961 gccgtccttg taaagactag aaaaagcttc atttatcttc ttaaccaagt ttgtatcttc
    10021 cttacgggct ccaaccgcaa aagcttctgt ttctagtcca actgtaaaga cattataatc
    10081 gtttaaaaca ccttctgctt ctaaataata gtttgcatag acacggtcaa tcaatagacc
    10141 atcaattcga tcgtttttca aatcaatcaa ggcttcatta aaggtttggt attgattcgc
    10201 ttccttatta gcgacaatat tcttcaaaat ttctggattt gcttcaaagt ccgcataacc
    10261 agatgaacca gcttgagctc ctaatgtctt tccagtcata tcctttgcag tcgtgatacc
    10321 agatgatttc ttcgtaacca atacctgctc attcttcata tatgagttac tgaaagccac
    10381 cttttcacgg cgttcgtctg tagcggaata gccattccaa atcagatcaa tcgttccttt
    10441 tgtcaattca gcttctttca aatcccaatc aatcggttgc caatttaccg tgattccgta
    10501 tttttcaaaa acagctgtag ctaaatcaat atcaaatcct gcataagaac catctttctg
    10561 agcaaatccc attggaacaa aagtactatc aaatccaata gtaatagact tgttagactg
    10621 gtactttgac caattatctc cactagtttc gctagaattt ttcccacaag ctactaagaa
    10681 caaagcagtc atcagactga ctaatacaag catccatttt ttcatccatt tttcctccta
    10741 tttaggttct actttcaata atacatctgc gatattttca gcaaactgca aatcatgggt
    10801 aaccacaatc tgggtcatcc caagttccct attttgcaag attagcttct ccacttccaa
    10861 acgtaattct ggatccaggg cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat
    10921 catcatagca cgcgccaaag ccacccgctg cttttgccca ccagatagtg agaaaggata
    10981 ggcctctgcg tgtcctccta gtcctaactg ttccaagagt ccactcgcct tcttctcagc
    11041 ctcttcctgc ttcattccca tggtcttcac aggcgataaa gtcaaatttt ccagaactga
    11101 tagatgagga aatagttgaa aatcttggaa gacaaatccc agtagattgc gcttctgcaa
    11161 ttcatccagc tctaaaggtt gtccattata aaagatttgc cctgaatcaa tggtttcaag
    11221 acctgcaagc atacgtaaaa gagttgtctt acctccacca gaaggtccaa cgatagccag
    11281 gatttgcttt tcaggaatac ttagactgaa attagacagg atttgtttgt ctccaaagac
    11341 tttattgata tttcgtaatt ctaacatggc agcctcctat ctataataac tatacttctt
    11401 ctcaactttt ttggaaataa ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc
    11461 caagaacata ggaactagac tagcatcgcg gttagcagct gttcgactag ccaagataag
    11521 gtctgaaatt ccgagagcat agaccaaaga agtatccttg accaaactca taacttcatt
    11581 aaagacacta ggaagaacga tcttggtcac ttggggcaag ataatatagc gcactctgtc
    11641 aaaagggcta aacttcaaga ccttagcggc ctcatactgt cctcttggaa tagtgtcaat
    11701 tcccccacgg aaaatttctg caaagtaagc tgcatagttg agaacaaagg caataatagc
    11761 tgcaggaagg cggtctaaac gaatcccaat acttgggagc acataataga taaaaatcag
    11821 ttgcaagagt aagggggttc ctcgcataac ccagatgtaa atgttaatca gataatggag
    11881 gggcttccaa tggacttgca aggcaaaggc aatcaaaacg cccaagggaa tcgaaaatag
    11941 caagaccagt gcaaagacct gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat
    12001 ctcaaataaa taagacatac tgcacctcct aaaagtaatt gttcctatta tagcatgaat
    12061 aaacaagatt accaagtatt tttgtaaaaa gattctacca tatacaaata aaaaccgtag
    12121 ctatcaaaag agataaacta cagttctata t
//
LOCUS       DBDESG010000062        12056 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.062, whole genome shotgun sequence.
ACCESSION   DBDESG010000062 DBDESG010000000
VERSION     DBDESG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.062"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(72..1274)
                     /gene="rpsA"
                     /locus_tag="ACKB38_001839"
     CDS             complement(72..1274)
                     /gene="rpsA"
                     /locus_tag="ACKB38_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGL7980557.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1436..1507)
                     /locus_tag="ACKB38_001840"
     tRNA            complement(1436..1507)
                     /locus_tag="ACKB38_001840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1473..1475),aa:Gln,seq:ttg)
     gene            complement(1517..1597)
                     /locus_tag="ACKB38_001841"
     tRNA            complement(1517..1597)
                     /locus_tag="ACKB38_001841"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1561..1563),aa:Tyr,seq:gta)
     gene            complement(1647..1877)
                     /locus_tag="ACKB38_001842"
     CDS             complement(1647..1877)
                     /locus_tag="ACKB38_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGL7980558.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2045..2689)
                     /gene="pcp"
                     /locus_tag="ACKB38_001843"
     CDS             complement(2045..2689)
                     /gene="pcp"
                     /locus_tag="ACKB38_001843"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGL7980559.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2704..3627)
                     /locus_tag="ACKB38_001844"
     CDS             complement(2704..3627)
                     /locus_tag="ACKB38_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGL7980560.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3624..4313)
                     /locus_tag="ACKB38_001845"
     CDS             complement(3624..4313)
                     /locus_tag="ACKB38_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGL7980561.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4378..5082)
                     /locus_tag="ACKB38_001846"
     CDS             complement(4378..5082)
                     /locus_tag="ACKB38_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGL7980562.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASHLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5138..6160)
                     /locus_tag="ACKB38_001847"
     CDS             complement(5138..6160)
                     /locus_tag="ACKB38_001847"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGL7980563.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6292..8763)
                     /gene="parC"
                     /locus_tag="ACKB38_001848"
     CDS             complement(6292..8763)
                     /gene="parC"
                     /locus_tag="ACKB38_001848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGL7980564.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVIIEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(8964..9168)
                     /locus_tag="ACKB38_001849"
                     /pseudo
     CDS             complement(8964..9168)
                     /locus_tag="ACKB38_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(9183..11126)
                     /gene="parE"
                     /locus_tag="ACKB38_001850"
     CDS             complement(9183..11126)
                     /gene="parE"
                     /locus_tag="ACKB38_001850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGL7980565.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTNE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEETTVF"
     gene            11262..11903
                     /gene="plsY"
                     /locus_tag="ACKB38_001851"
     CDS             11262..11903
                     /gene="plsY"
                     /locus_tag="ACKB38_001851"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGL7980566.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(11946..>12056)
                     /locus_tag="ACKB38_001852"
     CDS             complement(11946..>12056)
                     /locus_tag="ACKB38_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGL7980567.1"
                     /translation="QLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT         3467 a         2732 c         2119 g         3738 t
ORIGIN      
        1 actacagttg acaaagagcc agaaaaatcc cttgtttagt aaacaaagga ttttgtgaat
       61 tttcaatttg attaaagttc gatatcacca aacaaatcag ccattgaaaa tcctgtttgt
      121 gtttctggaa gttcgaaatc acgcttttct tgacgtcttg gacgacgtgg acgagcagca
      181 cgtttttctt ctttttgtcc ttcttcttgg gctggacgtt cttcaagagc tttaatagaa
      241 agtgacacgc gttctgcatc tgcgttaact tcaagaactt taacttgaac ttcttgacca
      301 actttaagag cttcttttgg attttcaatc cgtttgtgtg aaatttgtga tacgtgaaca
      361 agtccatcga tacctggcaa tacttcaaca aatgcaccga agtcagtcaa acgtttaact
      421 gttccttcta ctacatcacc tttagccaat ttttgctcaa cgccatccca tggtcctggt
      481 actgttgctt taagtgaaag tgatacacgt ccttcttctt cgttaagatc aaggattttc
      541 acttcaattt cttcaccaac agttacaact gattttggtg atacattacg ttcatgtgac
      601 aattcagtca agtgaaccaa tccgtcaaca ccaccaaggt cgacgaaagc gccgaagctt
      661 gtgatacgag caactttacc agttacaaca tcaccaacag ccaatttacc gaatacttca
      721 gcgcgagctg ctgcagtagc tgcttcaaca acttcacgac gtgaaaggat gaagcggttt
      781 tctttagcgt taacttcttt gattttagta tcaaattctt gacctacaaa acgctcagcg
      841 ttacgtacga aacgagtatc caacattgaa gctgggataa atccacgaac accttcaaat
      901 tctactgaaa gtccaccttt aacggcacgc gttcctttaa cagtaacaac ttcttcttcg
      961 cgaccaacaa gtttgtccca tgctttgcga gcttcaaggc gttttttaga tacaaggtat
     1021 gtaactgtat cagtatcttt accaactact tgacgaagta caagaacatc caatacttct
     1081 cctactttaa caaagtcatt gatatctgca tcacgatcgt ttgtcaattc gcgaagagtc
     1141 aagacacctt caacaccagt tccagagatt gcaacgttag cttgagtcgc atcaactgtc
     1201 aatacttcag cactaacaac atcaccagtc tcaacttgac taacgctatt tagcaaatct
     1261 tcaaattcgt tcatctaaaa aatcctccaa caatcaagtt tttcttaaac ttgacatact
     1321 tataattttt ttcctaagca cccgcaagga catgttcata tcattcacgt ctttcaatta
     1381 taaaaataaa ataccctaag atattttgct ttctatcttg attattacct aagaactggg
     1441 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
     1501 taccccatta atgaaatgga gagagaggga ttcgaacccc cgaacccgaa ggagcggatt
     1561 tacagtccgc cgcgtttagc ctcttcgcta tctctcctac aatcaacatg gactattata
     1621 tcatgaaaat ttcaaaaaaa caagacttat tttgctaaat tataattttc aatcaaaatt
     1681 tccacagctt tttccaattt tttataaaaa ctatcgacat tcccattctt tataatggca
     1741 aattgtccgt ctaattcggc aatttctccg ataacttttt taccgatagt caatgtataa
     1801 ccttcaaaac tgtctttacc aacattaact ttggcatccg ctacttgaat ttcgattttt
     1861 ttatctttct tgctcatttc atacctctct tcactttttc tattttacaa aaaaatccta
     1921 aaactagcaa gaaaaaactc tatatcaggc ttctctgata tagagtttta tgtaaacaac
     1981 ctcaattgat ttagcatagt taatagttag taaacaagca taattgacta tcctttttag
     2041 gttatcagtg agtctcaccg cctactaact gcaaatcctt atctttgtag tctacaatag
     2101 cttcaatagc tacctctatg cctcttacaa tatcacatag actcattcct gcagtattcg
     2161 gcttatttac tacttgttcc atcatatatg gaatatgcat gaaacctgct ctcatattag
     2221 ggaatttttt atccactaaa taaagagctt ggtacatcaa atgattgcaa acaaaggttc
     2281 ctgcactatt ggatacaact gccggaagtc cctgtttttt gatagcttgt accatcgctt
     2341 tgataggtaa actactaaaa taggccgatg ctccatcaat acgaatcggt gtatcaattg
     2401 gttgattgcc ttcgttatca ggtatgcgag catcatcttg attaatagcc actcgttcag
     2461 gtgttaagcc ggtcctgccg cctgcttgtc caatacaaag tacagcatct ggttgatatc
     2521 gtaatatttc tgcctctaaa acttctgacg acttataaaa aaccgttgga atttctaccc
     2581 agcgaacttc agccccatta atctcagatg gtaataattt tacagcctcc aaagctggat
     2641 taatcttttc acctccaaaa ggattaaaac ctgtaaccaa tattttcatt ttattttcct
     2701 ttactaaaat gcgagaaagt acattaagaa tatgtgaata acaatcatta ctagagcaac
     2761 acctgcttga gcctttataa cgccattctg atctttcata tccatcaatg ctgctggtag
     2821 agcgttaaaa ttagcagcca ttggggtcaa taaggtccca caataacctg ctgtcatggc
     2881 aagagcacca gccacaattg gattagctcc cagagcaaat acaaagggaa ctccaacacc
     2941 tgctgtaata acggtgaatg ctgcaaaagc atttcccata atcattgtga atagaaccat
     3001 tccaagaaca taggccaaaa ctcctataaa gcgactatct gaaggaacaa taccgctaat
     3061 cagatgagag ataacatcac caacacctgc tacagtaaaa atagccccca aagcccctaa
     3121 taattgagga acaatcccac ttgttgaaac ttgctgagtc attcgattat tttctgataa
     3181 cagactctta gggtgactat tggtaatcac aagaacagaa attgtagcaa acaaggcggc
     3241 aaggctaatc gaaatcttgc taaattctgg aatcatttgc gctaagacca acgcaagtat
     3301 tgccatcagc ataactggaa taaaaatttt atttttcaac ctgttagatt caatattggc
     3361 tttcatttca tctaaggatg gcaaggttcc gatacggact tgcttaaaca atgttaacag
     3421 cgataatagg attacaataa taccaatact catatttggc atataggaac cacctataaa
     3481 cgtaatagac aatagagtcc aaaatgcaga tgtcccaagt cgaactgggt ttgttttatc
     3541 tttataacta caataggctg tatggagaaa ttgacaacca atcacaatat aggtcaactc
     3601 taatagttgc tttgccaact ctgtcatttt tgttctcctc ccctagtctt ttttgatatc
     3661 aattttttat caaataaata attataaatc cccactacaa taagtgttat aacagcaaca
     3721 ataatagatg tagaagcaat ccctgcataa ttgctttcat agcctaactg atctaatgtt
     3781 ccccctatca agaggactcc cccagcacct acaaacgtat tttgagcaaa gaaatttcca
     3841 aaattttcat tcgcagccgc acgcgctttt attgtctcat cttcaacctc tgttaacttt
     3901 ctacctaatt gagactctgc agctgcttct cccataggtt gaaccaaagg tctgacaaac
     3961 tgagggtgtc ctcctagacg aattgaaaag aaaccagcta actctcgaat aaagaaataa
     4021 actgtataga agtttccaac tgtcagacct ttaatctttc gaatcaaatc gattgatcgt
     4081 tgcttgagtc caaaggtttc tgacagcccc acaagaggca aggtaaccat aaaaatcgtg
     4141 agcactcgct gattgctaaa ttcttttccc aaaatctcca aaaattcaac gagagaaaca
     4201 cctgaaacta aagctgtaac caaaccagct aagactactg ttgcaattgt atcaaatttt
     4261 aaaataaaac ccacaacaat gattgctatt cctattaatc taatccactc catatcaaac
     4321 tcctttatat tcaaaatgac agtattttta aaattttatc aagatcaata ccattcctta
     4381 tttaatgtgt ttttctagtt ctttttggta tttgctattg gattccaatt tttctttttg
     4441 ccatttttta aaaacctcgt tatattcttt tgttgtaaca atatcttttt gcaatttcat
     4501 tcctttaaag atatatggat cccccttaat accaacttgt gagtatggtt ttgagaatgg
     4561 tactacgtta cttacaactg gagaaccacc agatgaagct gttggcatca ataatgaact
     4621 atctgtcgac caagcttgag ctttggcata tttttcatat cttttctcta ggtcagtggt
     4681 ctcagaaaca gcatcttcta acaatttctt atatttatcc aaaccaggtt tagctacaac
     4741 atccttatct tttcctttcg taataccaag gtgtttcatg gcagaaccag attttggatc
     4801 tataatattc aagtgagacg ctggatcttg atagcttgga gcccatcctg tactgttcaa
     4861 atcatagtct ttttgagaag gagcaacatt gccgtattta tcattttcca tcaaaccatc
     4921 aataacattt ccaataacgt ctgtcctcga tgttcgagtc gctatactgt agcccaatga
     4981 tgctggatct actgcataga cataagaaaa tgttgtcggt gcatctgctt ctttatcagt
     5041 ttttccacaa gccactaaaa tagctgacgt gctcaggacc actcctgctg ttaagagcca
     5101 ctttttctat ttcataaaga atctcctttg gtttatttta atctactttt acaatccaac
     5161 cttctggcgc ttcaatatcg ccaaactgaa tacccgtcaa ttcattatat aatttacgcg
     5221 tcacaggacc tacttctgtt tcactataga atacatggaa atcatcacca tgttgaatac
     5281 ctccaattgg agaaataacc gctgctgtac cacaggcacc tgcctctaca aaacggtcaa
     5341 gattatcaat tggaacatca ccctcaatag gagttaatcc caagcgatgt tctgccaaat
     5401 aaagcaagga atacttggta atagatggca agatagatgg actcaatggt gttacaaatt
     5461 cattatcagc tgtaattcca aagaagttag ctgatccgac ttcttcaatc tttgtatgag
     5521 ttgatgggtc cagatagata acatctgaga aatgacgtga cttggccatt tttcctggta
     5581 agagacttgc agcatagttt ccaccaacct tagccgcacc tgtaccattt ggtgctgcac
     5641 ggtcgtactc atcctgaatc aagaagttgg ttgggaccaa accaccttta aagtaatttc
     5701 caactggcat agcaaagatg gtgaaaatgt actcttctgc cggttttacc ccgataatat
     5761 ctccgacacc aatcaaaaga gggcgaagat ataaggttcc acctgttccg tatggtggta
     5821 cgtattcttc attcgcacgg acaactgctt tacaagcttc tacaaacatg tctgtcggaa
     5881 cttgtggcat caagagacgg tcacatgtac gttgcagacg tttagcattt tcatcaggac
     5941 ggaacagttg aacactgcca tccttagtac gataagcttt caaaccttca aatgcttgtt
     6001 gtccatagtg aagacttgga gaagactctg aaatatgcaa agttgcatcc tctgtaagct
     6061 ctccttgatc ccattgtcca tttttgaaat gagcaagata gcgataaggt aatttcatat
     6121 aggaaaaacc gaggttttcc caatcaatcg ttactgtcat attccactcc ttattctaaa
     6181 aacctatttc ttatattcta cactattttt ctaaaatagc aagtatattt tgtaattttc
     6241 agaaaatttc tccaataaaa accaactctt agaactgatt cttcatttca cttatttatc
     6301 ttcagtaact acttcctgaa gataagcgtc aaaaacttct tcatctgaaa tcgtgtcaga
     6361 aatgaagctt ccattgctag tgcgttctga caagttcaag tcttgcaatc ggctttcata
     6421 gattgttcct ttattggatt ggacaagcag agtttggtcg ttcacatcca cttccgtact
     6481 gaagaaatcg ccaacaaatc cttgctctgc aactgctcct gccaagaaga cacgatgcgg
     6541 tttgtttttc aactcacgca agacttgtaa tcctcgtttg gcacggctgg ttgctagaat
     6601 ttcctcaatg gaaacacgtt tcaagcttcc acgctgggtc aagaggtaga aggacgaagt
     6661 attacagata aagccagatt ggaggacatc atcttctttc aaattcatag ccttgacacc
     6721 tgctgcctta gcaccgacaa ccggaacctc ttcgatattg aaacgcaggg cataaccatt
     6781 ttgactaacc aagacaacat catctagttt aatcggagcc actgctacaa tctgatctgt
     6841 atcgtctttg agcttagcat acttgacaga cttagatcta taggtccgcc atggagtgaa
     6901 ttcttttcgc tctacccgtt tgatttgacc aaggcgagtc actgcaaagt aggttgtcgc
     6961 atcgtcaaac tgatccagta cttccacata aaggatttct tcattcgttt caaagtttgt
     7021 gatggtttgg ctcagatgct ctccgatgtc cttccaacga atatctgcca actcatggat
     7081 tggtctgtag atgacatttc caagacttgt gaacatcaag aggtgctggg ttgtcttggc
     7141 agattgaaca aaaatcaaac ggtcatcatc acgcttgcca atttcttcca aggtggaagc
     7201 cgcaaaggaa cgtggactgg tacgcttgat gtaacctgcc ttggtcacgc tgacgtaggt
     7261 atcttcctca gcgataagac tagctgtatc aatctcaatt gctttcgcag tgtcttctaa
     7321 agaactcaaa cgaggagttg caaatttctt cttgacctca cgaagttctt tcttcatgag
     7381 attgtacata gtcctttcat caccgataat agccgccagc atagcaatct tctcacgaag
     7441 ctctgcttct tcttcctgca agacaaccac atcggtattg gtcaaacggt acagttgcaa
     7501 agttacgata gcctcagcct gttcttccgt aaaatcatag ctaactttga ggttttcctt
     7561 ggcgtccgcc ttattctcag aagcacggat aagagcaatg acttcatcca aaatcgaaat
     7621 cacacgaatc aaaccttcga cgatatggag acgtttctca gccttttctt tgtcaaagcg
     7681 tgaacgcgcc aaaatcactt ctcgacggtg agcgatatag ctagacagga ttggaacaat
     7741 cccaacctga cgaggtgtga aattgtcaat cgccaccata ttaaagttgt agttgatttg
     7801 taggtcggtg tacttaaata agtagttgag aacaagctca gtattagcgt ctttcttaag
     7861 ttcgatagcg atacgaagac catcacggtc agactcatca cgaacctcag caatcccagc
     7921 taccttgtta ttaacacgaa catcatcgat tttcttgact agattggcct tattgatttc
     7981 ataaggaatc tcaataataa cgatttgttc cttaccacct tttagctttt caatttcagt
     8041 cttggaacga acaaccacgc gccctttccc agtctcataa gctttcttga tttcatcacg
     8101 accctgaata atagcccctg tagggaagtc tggtccaggc aagaattcca tgagtttatc
     8161 aatctttgca gttgggtggt caatcatgta aactgcagca tctatgacct cagctaaatt
     8221 atggggagga atgtctgtgg cataaccagc cgaaatccca gtcgaaccat tgaccaagag
     8281 gtttggaaag gctgctggca agaccgttgg ttctttctcc gtatcgtcaa agttccatgc
     8341 aaaaggaact gtctttttct cgatatcctg aagaaggtag cctgcaattt cagacaaacg
     8401 tgcctcagta taacgcatag ccgcaggagg atctccgtcc atagaaccgt tattaccgtg
     8461 catttcaact agaatctcac gatttttcca gttctgtgac atacgaacca tggcatcata
     8521 gatagaagaa tccccgtgtg ggtggaaatt ccccatgatg ttcccgactg acttggccga
     8581 cttacggtag ctcttgtcaa aagtattgct atccttattc atagaataaa gaatacggcg
     8641 ctgaaccggc ttcaacccat cacgaatatc tggcaaagcc cggtcttgaa taatgtactt
     8701 ggagtagcga ccaaagcgct ctcccatgat gtcctccagg gacatgtttt gaatgttaga
     8761 cataagatac aaagcccata aaataccaag tgaaaataga aaattcttga agtaagcaaa
     8821 ctcacaagag aatttatctt tttcacacag tatctagggc gtgttcaact cctttcaaag
     8881 aatgtagagt aggtttttat gcagtaaaag atattttacg ggaattcctc ccgtgttcag
     8941 ttacgataag taaccaaact atcctgtttg tatttttcaa tatgaaaatc tggttttcca
     9001 aaattagtct tagtttgtgt cttagccgct cccttaagcg cctctttgag ataagcactc
     9061 atagcagatt cttcattaat aatcctgcaa ttttttcaaa ccaagatttt caaactgctt
     9121 tttcacatag tcattcacat ccgactctaa tttccagttt actaacatat tattttcttt
     9181 cattaaaaca ctgtcgtttc ttctagcgta aacttgacat tatcttcaat ccatttacgg
     9241 cgtggttcta ccttatctcc catgagaaca ttgacgcggc gttcggcgcg cgctaaatct
     9301 tcaattgtga cacggatgag ggtacgtgtt tctgggttca tggttgtttc ccagagctgg
     9361 tccgcattca tctcaccaag tcctttgtat cgttggaggg tagcgccttt accgaactgt
     9421 ttacggagtt cttctagttc tccgtccgtc caagcgtagg ccacttcttc tttcttgcct
     9481 ttacctttgg acatcttgta aagaggtggg agggcaatat agacatgacc tgcctcgact
     9541 agcggacgca tgtaacggta gaaaaatgtc aagagcaagg tctggatatg ggcaccgtcg
     9601 gtatccgcat cggtcatgat aatgatctta tcatagttgg catcttcaat agagaagtct
     9661 gctccaacac ccgcaccaat ggtataaatc atggtattga tctcttcatt tttgaggata
     9721 tccgccatct tggccttggc tgtattgaca accttaccac gaagaggtag aatagcctgg
     9781 aacttgcggt cacgaccttg tttggcagaa ccaccggcag agtccccctc aactagatag
     9841 agttcattct tagcaggatt cttagattgg gctggggtca atttcccaga caacaagccc
     9901 ttatctttct tgtttttctt cccatttcgg ctctcatcac gcgccttacg tgctgcttca
     9961 cgagcatcac gggccttgat agccttgcgg atgaggttag aagctaattc cccattttcc
    10021 ataaggaaaa aggtcaactt atcagccact attccatcca caactgggcg agctaggggg
    10081 cttcctagtt tatccttggt ctgtccttca aactgcaagt gttcttcagg aactaagata
    10141 gaaagaacgg ccgctagtcc ctcacgatag tctgaacctt caaggttttt atctttttcc
    10201 ttgagaagac ctgttttacg tgcatagtca ttcatgacct tggtaatggc agacttgagt
    10261 cctgtctcgt gcgttccacc gtccttggtg cgaacgttat tgacaaaaga tagaatgtta
    10321 tctgagaatc cgtcattgta ctggagggct acttccactt gaaaaccatt gtcttcccct
    10381 tcaaagtaaa gaactggcgt caagatttcc ttatcttcgt tgagataaga aacaaaatct
    10441 tgtactccat tctcatagtg gaactcaatc gcttcatttg ttcgcttgtc cgttaaagac
    10501 aaggtcacat ttttcaagag aaaggctgat tcattaaggc gctctgaaat ggtattgtac
    10561 ttgaaatctg tcgtagaaaa tatagtcgcg tcaggcataa aagtaacttt ggtgcctgtt
    10621 ttagacttgg gtgctgtacc gattttcttc aaagtcgtga caggttttcc accattttcg
    10681 aaacgttgct tgtaaactgc gccatcacgg gtaatttcaa cttctaacca gctagaaagg
    10741 gcgttaacaa cggaagaacc cactccgtga agtccacctg atgtcttata gccaccttga
    10801 ccgaatttcc ctccggcatg aagaatggta aagataacct caacagttgg aattcccata
    10861 gcgtgcatac ctgtcggcat cccacgtcca tggtcttgaa ccgttagact accgtcttta
    10921 ttgatagtta catcaatacg atcaccaaac ccagacaagg cttcatcgac tgcattatca
    10981 acgatttccc aaactaggtg atgaagacca gcgccatcgg tcgatccaat atacatccct
    11041 ggacgttttc ggaccgcatc caacccttct agcacctgaa tagcatcatc attataattg
    11101 ttaatattga tttccttttt tgacacaagg aacctcctat tcgttcatct ttactattct
    11161 acaggttttc caaggatttt gcaaaatttt tctttctccg atgtgacaat ttcagcagag
    11221 attctctgct tttctttccc aattcatgat ataataggag tatgattaca atagttttat
    11281 taatcctagc ctatctgctg ggttcgattc catctggtct ctggattgga caagtattct
    11341 ttcaaatcaa tctacgcgag catggttctg gtaacactgg aacgaccaac accttccgca
    11401 ttttaggtaa gaaagctggt atggcaacct ttgtgattga ctttttcaaa ggaaccctag
    11461 caacgctgct tccgattatt tttcatctac aaggcgtttc tcctctcatc tttggacttt
    11521 tggctgttat cggccatacc ttccctatct ttgcaggatt taaaggtggt aaggctgtcg
    11581 caaccagtgc tggagtgatt ttcggatttg cgcctatctt ctgtctctac cttgcgatta
    11641 tcttctttgg agctctctat cttggcagta tgatttcact gtctagtgtc acagcatcga
    11701 ttgcggctgt tatcggggtt ctgctctttc cactttttgg ttttatcctg agtaactatg
    11761 actctctctt catcgctatt atcttagcac ttgctagttt gattatcatt cgtcataagg
    11821 acaatatagc tcgtatcaaa aataaaactg aaaatttggt cccttgggga ttgaacctaa
    11881 cccatcaaga tcctaaaaaa taaaatgcca gttctgtact gcccccaaac agttagacaa
    11941 ataatttatc caaaggattt agttctgtac tgcacaggac taagtccttt tagttttacc
    12001 ttaattcgtt tgttgttgta gtaatcaata tagtctataa tggcttgttc caattg
//
LOCUS       DBDESG010000063        12052 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.063, whole genome shotgun sequence.
ACCESSION   DBDESG010000063 DBDESG010000000
VERSION     DBDESG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.063"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..175
                     /locus_tag="ACKB38_001853"
     CDS             <1..175
                     /locus_tag="ACKB38_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGL7980568.1"
                     /translation="EDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRM
                     NLSAGIINHELGF"
     gene            366..2657
                     /gene="pcrA"
                     /locus_tag="ACKB38_001854"
     CDS             366..2657
                     /gene="pcrA"
                     /locus_tag="ACKB38_001854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGL7980569.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNSQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2700..3380
                     /gene="radC"
                     /locus_tag="ACKB38_001855"
     CDS             2700..3380
                     /gene="radC"
                     /locus_tag="ACKB38_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGL7980570.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3377..4066)
                     /locus_tag="ACKB38_001856"
     CDS             complement(3377..4066)
                     /locus_tag="ACKB38_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGL7980571.1"
                     /translation="MKKPVIGITGNEKTHPDNDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4084..4725)
                     /locus_tag="ACKB38_001857"
     CDS             complement(4084..4725)
                     /locus_tag="ACKB38_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGL7980572.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4808..5023)
                     /locus_tag="ACKB38_001858"
     CDS             complement(4808..5023)
                     /locus_tag="ACKB38_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGL7980573.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5031..5378)
                     /locus_tag="ACKB38_001859"
     CDS             complement(5031..5378)
                     /locus_tag="ACKB38_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGL7980574.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5383..6498)
                     /locus_tag="ACKB38_001860"
     CDS             complement(5383..6498)
                     /locus_tag="ACKB38_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGL7980575.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFVNNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6508..7467)
                     /locus_tag="ACKB38_001861"
     CDS             complement(6508..7467)
                     /locus_tag="ACKB38_001861"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGL7980576.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7580..8149)
                     /locus_tag="ACKB38_001862"
     CDS             complement(7580..8149)
                     /locus_tag="ACKB38_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGL7980577.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8277..8948
                     /locus_tag="ACKB38_001863"
     CDS             8277..8948
                     /locus_tag="ACKB38_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGL7980578.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8932..9750
                     /locus_tag="ACKB38_001864"
     CDS             8932..9750
                     /locus_tag="ACKB38_001864"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGL7980579.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDLLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9747..10643
                     /locus_tag="ACKB38_001865"
     CDS             9747..10643
                     /locus_tag="ACKB38_001865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGL7980580.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10687..11661
                     /gene="pta"
                     /locus_tag="ACKB38_001866"
     CDS             10687..11661
                     /gene="pta"
                     /locus_tag="ACKB38_001866"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGL7980581.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(11923..>12052)
                     /locus_tag="ACKB38_001867"
     CDS             complement(11923..>12052)
                     /locus_tag="ACKB38_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980582.1"
                     /translation="NNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERMKFVLSRA"
BASE COUNT         3524 a         2305 c         2588 g         3635 t
ORIGIN      
        1 tgaagataaa gcctataacc atgcgctatc taaggaaaga agcaaggttg agaacatctt
       61 tgccaaagta aaaacgttta aaatattttc aacaacctat cgaaatcatc gtaaacgctt
      121 cggattacga atgaatttga gtgctggtat tatcaatcat gaactaggat tctagttttg
      181 caggaagtct attgaggtat tgagctagtt tatgaaaaaa ttgggtgaaa agtcgagtgt
      241 tttagaaacc cacagtgtag tattctagtt tcaatccact atattttgct actccccgta
      301 aagtttctat tttccctgat ttctgatata atagaaatat tgacttcaag agtaaggaag
      361 agaagatgaa cgcattatta aatggaatga atgaccgtca ggctgaggcg gtgcaaacga
      421 cagaaggtcc cttgctaatc atggcagggg ctggttctgg aaagactcgt gttttgaccc
      481 accgtatcgc ttatttgatt gatgaaaagc tggtcaatcc ttggaatatc ttggccatta
      541 cctttaccaa caaggctgcg cgtgagatga aagagcgtgc ttatagcctc aatccagcga
      601 ctcaggactg tctgattgcg accttccact ccatgtgtgt gcgtattttg cgtcgcgatg
      661 cggaccatat tggctacaat cgtaatttta caattgtgga tcctggtgaa cagcgaacgc
      721 tcatgaaacg tattctcaaa cagttgaact tggaccctaa aaaatggaat gaacgaacta
      781 ttttggggac catttccaat gctaagaatg atttgattga tgatgttgct tatgctgccc
      841 aagctggcga tatgtatacg caaattgtgg cccagtgtta tacagcctat caaaaagaac
      901 ttcgtcagtc tgaatccgtt gactttgatg atttgattat gctgaccttg cgtctctttg
      961 atcaaaatcc tgatgttttg acctactacc agcaaaaatt ccaatacatc cacgttgatg
     1021 agtaccaaga taccaaccac gctcagtacc aattggtcaa actcttggct tcccgtttta
     1081 aaaatatctg tgtggttggg gatgcggacc agtctatcta cggttggcgt ggtgctgata
     1141 tgcagaatat cttggacttt gaaaaggatt accccaaagc caaggttgtt ttgttggagg
     1201 aaaattaccg ctcaaccaaa accattctcc aagcggccaa cgaggttatt aaaaataata
     1261 aaaatcgccg tcctaaaaat ctctggactc aaaacgctga tggggagcaa atcgtttact
     1321 atcgtgccga tgatgagctg gatgaggctg tatttgtagc cagaaccatc gatgaactta
     1381 gtcgcagtca aaacttcctt cataaggatt ttgcagttct ctatcggact aatgcccagt
     1441 cccgtacaat tgaggaagcc ctgctcaagt ctaacattcc ttataccatg gttggcggaa
     1501 ccaaattcta cagccgtaag gaaattcgcg atattattgc ttatctcaac cttattgcta
     1561 atttgagtga caatattagt tttgagcgta ttatcaacga gcctaaacgt ggaattggtc
     1621 taggtacagt tgagaaaatc cgtgattttg caaatttgca aaatatgtct atgctggatg
     1681 cttctgctaa tattatgttg tctggtatca agggtaaggc agcccaatct atctgggatt
     1741 ttgccaatat gatgcttgat ttgcgggagc agctagacca cttaagcatt acagagttgg
     1801 ttgagtccgt cctagaaaaa acaggttatg tcgatattct taactcccaa gcgactctag
     1861 aaagcaaggc acgggttgaa aatatcgaag agtttctttc tgttacgaag aactttgatg
     1921 acaccacgga tgtgacagaa gaggaaactg gtctggacaa actgagtcgt ttcttaaatg
     1981 acttggcttt gattgccgac acagattcag gtagtcagga gacatcagaa gtgaccttga
     2041 tgaccctgca tgctgccaaa ggtctcgaat ttccagttgt ctttttgatt gggatggaag
     2101 aaaatgtctt tccacttagt cgtgcgactg aagattcaga tgaattagaa gaagagcgcc
     2161 gtctagccta tgtaggtatc acgcgtgcag agaaaattct ctatctgacc aatgccaact
     2221 cacgcttgct ttttggtcgt accaattata accgtccgac tcgttttatt aacgaaatca
     2281 gttcagactt gcttgagtat caaggtctgg ctcgtcctgc aaatacaagc tttaaggcat
     2341 catatagcag tggtagtatt tcctttggtc aaggtatgag tttggctcag gctcttcaag
     2401 accgtaaacg cggtgctgcc ccaaaatcaa tccagtcaag cggtcttcca tttggtcaat
     2461 ttacagctgg cgcaaaacca gcatctagcg aggcaaattg gtccattggt gatattgctc
     2521 tccacaagaa atggggagag ggaaccgttc tggaagtttc aggtagcggt gctaggcagg
     2581 aattgaaaat caatttccca gaagtaggtt tgaaaaaact tttagccagt gtggctccaa
     2641 ttgagaaaaa aatctaattt tccatccttc tcacgaataa taaagtgagg aggattttta
     2701 tgtacagtat ttcattccaa gaagattcac tattaccaag agaaaggctg gccaaggaag
     2761 gagttgaagc gcttagtaac caagagttgc tagctatttt actcaggaca ggaacacgtc
     2821 aagctagcgt ttttgaaatt gcccaaaaag tcttgaacaa tctttcaagc ctaacggatt
     2881 tgaaaaaaat gaccctgcag gaattgcaga gtttgtctgg tattgggcgt gttaaggcca
     2941 tagaattaca agctatgatt gaactggggc atcgtattca caaacacgag actcttgaaa
     3001 tggaaagtat tctcagcagt caaaagttgg ccaagaagat gcagcaggaa ttaggggata
     3061 aaaaacaaga gcacctggtg gcactctatc tcaatactca aaatcaaatc atccatcagc
     3121 agaccatttt tatcgggtct gtaactcgta gtatcgctga accgcgagag attcttcact
     3181 atgcaatcaa gcatatggcg acttctctta tcttggtcca caatcatcct tcaggagcgg
     3241 tagcgcctag ccaaaatgat gatcatgtca ctaaacttgt taaagaagcc tgcgaattga
     3301 tggggattgt tctcttggac catttgattg tctctcattc taattacttt agttatcgtg
     3361 aaaagacaga tttaatctaa agttcattaa cgacatagtc aaagagtttt ttatctttgg
     3421 gacgattttc aaaaagaagt tctggatgcc attggacacc gagaaaggcg acatcatccg
     3481 tactcatgac agcctcaatg ataccatctt taggatcatg agccacaact tttaaatttg
     3541 gtgctaagtc cttgatgctc tggtggtgga aggagttgat atgagagatt tctccataga
     3601 tttcttggag aacggtatct ggttctgtta ccaagcgttg agttgtgtac tcaacagaag
     3661 aatcctgcca atggtcttcg atatcttggt acaaagttcc acccatggca acgttaaaga
     3721 gttgggtacc acggcagaca gagaaaatgg gctttttctg tttaatagct tccttgatga
     3781 gggccagttc gaagatatct ctttgaaggt gatagtcatc actatcaatg gttttgggtt
     3841 cgccataaaa ttttggatcg acattttgcc cacctgtcaa gatgagcttg tcaatcaaac
     3901 tgatatagtg gcaggccatt tcttgatcac caatcggtag gatgatggga atccctccag
     3961 catctttaac gccttcaaca aagccttttg ctgcgtagct catcatgatg tcattatctg
     4021 gatgagtttt ttcgtttcct gtaatcccaa taactggttt tttcataaaa tgattttcgc
     4081 tttctaatcc tcttttcgca tgaagtagag gagggtttgg agttcacttg tcaaatcgac
     4141 atactgaacg accacgtctt ttggtaaatg cagatggact ggtgaaaaac tgagaattcc
     4201 tttcacacca gcatcaacca agagattagc aacctcttgt gacttgacgc tgggaacagt
     4261 taggatagca gtcttcacat cagcatcctt gattttatcc ttgatctgag aaatcccgta
     4321 aatgggaatc ccgtcaggag tttgggtacc gacttcagga tggtcgtcta ggtcaaaggc
     4381 catgataatc ttcatcttgt tacgttcgtg gaagcggtag tggagaaggg catggcccat
     4441 atttccaata ccaaccagca tgacattggt aatagagttg tcattgagca aatcggcaaa
     4501 aaatgtcatt agttttttga catcatagcc aaaaccacga cgaccaagtt caccaaaata
     4561 ggaaaaatca cgacgtacgg tcgctgaatc aataccgata gcctctgcaa tttgcttaga
     4621 gttggcacgt tcaatctttt ctgcatgaaa tctcttaaaa attcgatagt agagagagag
     4681 tctttttgct gtagcttttg gaatagcaaa ctgtttatct ttcacaaaat cacaaccttt
     4741 ctattcttct attttataga aacattgtga aaaaatcaac aaaaataaga aaaaactaag
     4801 aaaaatctta gttttgatgt aaaaaatctg catgagatag aaaacggtag aggtctccga
     4861 ccagcccctg ataaactttt ttgcccctaa aagtcagaga agtcacataa agtgtatctg
     4921 gtaaggttac acatcctgac aaagtcaaca tgagagcctc atgatcctca tacttgagag
     4981 tacgctctac atgatagcag tccttatagg tcagttcaaa cattttggct ctatctttcc
     5041 gattttgtaa agacaccacg ttctaccaag ctatccatga ggaagtagaa tttttcctga
     5101 tgaatatggt ggtcttctga tttgaaaata tcaactagac gaaggccaaa cttgtcagtg
     5161 atattgattt tagcccctgt aagttccttg ttaatgatga ttttgagttg gaagccttca
     5221 ccgctgtttg gcactttttc caaaaggcga gtcagttcat agttaccaac cttagtttca
     5281 aaaaaggtgt tatctttgag ggtgaatttt ttaacagaag ggctaagagt gtaatcgtaa
     5341 cgacaatttt ttaactgaat gattttttca aatgccatat ggctaacctc cgataatttc
     5401 ttttaaggtt tttgcgaggg tttgtaggtc ttcaacggta ttttgtggcg acaaactgat
     5461 gcgaagggat tccttcaagc gttctgaatt tgcgccatac atggcttcaa gaacatggct
     5521 ggattggaca acgcctgcag tacaggctga gccagtagag attgaaattc cagctaaatc
     5581 tagccgaagg agtaagaggt catttttctg accaggaaat ccaatattga gaacataagg
     5641 gagatgatgt tttcctctat tcaggtaata ctgaatgccc tccagctctg ccagaaaggc
     5701 agtttctaga ttttgtacat gttgaaaatg ttcttcttgt ttttctaggt cttcttttag
     5761 ggctgcaacc atgcctacaa tggcaggcag attttcagtt cctgcacgtt ttttctgttc
     5821 ctggtctccg ccatgtagat aggaatcaaa gtccatgcta gatgcgtaga gaaaaccgat
     5881 tcccttagga ccatggaatt tgtgggcaga agcagtgaga aaatcaatgc ccaattcttc
     5941 tgaatgaatt gggattttac caatagcctg aactgcatca acatgatagg cagcagggtg
     6001 ttgcttgagt atttggccaa tttcagcgat gggcagtagg tttcctgtct cattattgac
     6061 aaacatggta gaaaccaaaa tcgtatcgtc acgtaaagcc ttttgaattt gctgggctgt
     6121 gatttcttga ttttctggct ggataatggt tgcttcaaac ccaaagtgtt gaaccaagta
     6181 atcaattgtt tcaaggacag catggtgctc gatggcagtt gtgatgatat gttttccttg
     6241 ttcttggtga cgaagacagt agccaatgat ggtagtatta ttgccttcag tcccaccaga
     6301 agtgaaaaag atatgttgag gttttgtcct tagtaactgg gctagttcct gacgggcttc
     6361 tcgcaagagt ttgccagctt gacgaccatg accatgaata ctagaaggat ttccgtgggt
     6421 ttcttgcata accttggtca tagctgaaat agcaactgct gacataggag tcgttgcagc
     6481 attgtccaaa taaatcaaag aatcacctta tttcttttta ttgtaggcaa agagtgggct
     6541 gactggtttt ctttcgtgaa tacggacgat agcatcacca attaactcac tagcagtgat
     6601 gtagcataca tttttaggag ttttttcttt tgttgctact gaatcagtca caagaatttc
     6661 tttaatatta gtattgtcaa gaagctcagc agctccctcg acgaagagac cgtggctaga
     6721 aacagcataa atttctgtag ctccttcacg ttcaacgatt ttagaagctt cagagaaggt
     6781 acgtcctgta tttaaaatat catcaatcaa gatagctttc ttaccttcaa catcaccaat
     6841 aatataacct tcgttacgag ttgcatcgtc ttgagggtag tcgataatgg cgataggagc
     6901 atcaagatat tcagccaggc tacgcgcacg tttgacacct gaatttttag ggctaacgac
     6961 aacaacatct gaaccaagca atcctttatc gcagtaatgt tttgcgaata ggggaacagt
     7021 gaaaagatta tccactggaa tatcaaagaa accttgaacc tgaacggcat gcaaatcaag
     7081 agtcaggata cgatcaactc cagccttaac cagcatattg gcaactagtt ttgctgtaag
     7141 tggctcacga ggacaagcaa tgcggtcttg acgtgcatag ccaaaatatg gaaggacaac
     7201 gttgatactg tgggcacttg cacgcacaca agcatcgacc atgattaaca attccattag
     7261 gtggttgttg acagggaaac ttgttgattg gatgatgtaa acatcataac cacggacact
     7321 ttcttcgata tttacttgga tttctccgtc tgaaaattga cgtgatgata gttttccaag
     7381 tgggacacca acagcttggg caattttttg tgcaatctct tggttagagt tgagtgcgaa
     7441 aagtttcatg ttttttctat ctgacattat agaccgtcct ctgtaaactt tataaatcct
     7501 agttatattt accttacata tatgaactgg gatttgtgta tttttatctt ttctatttta
     7561 ccaaaaaatg gagattattt cagctatttt tcatactttt gacaaatcga accaattttg
     7621 aaggagcttt ttgataggaa atctgatttt tctctaaaaa ttgtcgaaaa tcctgtttgc
     7681 cttgctcatg attttccact tcaagctcca attcgtaatc tgttatatca aagtatcggc
     7741 tctgatccag tgccatgaga ccaatagctg ttttcatttc atagcgaagc gttgttagac
     7801 aaccaagaac ctgccagttc ttactttgga taccatgttt cgccaattca tccagtacta
     7861 gcccttgagg aagttcttcc ttactcagat agttctcagc atcttttagt tgcaattttt
     7921 ggttgtattc catgtttcca acactctgcg ggactttgag tgtcaactca gcccagtctt
     7981 caaaggttcg aatgcgcata gcgactttct tttctcgcag ttcaaaatca ggcgtgtcga
     8041 tgtagtaatt tgtttgaaga acaggagtga cacctgtgaa ctggtctttt agacgattgt
     8101 attcatcttt tttcaatagt gttttcaatt caatttctaa atgtttcatt tttcttacct
     8161 ttttttatcg ttgaaagcgg atttatggta taataagcat tgtatttatt gtatatgaat
     8221 ctggagaaaa aatcaaagat atttttgacg gataatatga gaacaaggga gaatatatga
     8281 ccttagaatg ggaagaattt ctagatcctt acattcaagc tgttggtgag ttaaagatta
     8341 aacttcgtgg tattcgtaag caatatcgta agcaaaataa gcattctcca attgagtttg
     8401 tgaccggtcg agtcaagcca attgagagca tcaaagaaaa aatggctcgt cgtggcatta
     8461 cttatgcgac cttggaacac gatttgcagg atattgctgg cttacgtgtg atggttcagt
     8521 ttgtagatga cgtcaaggaa gtagtggata ttttgcacaa gcgtcaggat atgcgaatca
     8581 tacaggagcg agattacatt actcatagaa aagcatcagg ctatcgttcc tatcatgtgg
     8641 tagtagaata tacggttgat accatcaatg gagctaagac tattttggca gaaattcaaa
     8701 ttcgtacttt ggccatgaat ttctgggcaa cgatagaaca ttctctcaac tacaagtacc
     8761 aaggggattt cccagatgag attaagaagc gactggaaat tacagctaga atcgcccatc
     8821 agttggatga agaaatgggt gaaattcgtg atgatatcca agaagcccag gcactttttg
     8881 atcctttgag tagaaaatta aatgacggtg taggaaacag tgacgataca gatgaagaat
     8941 acaggtaaac gaattgatct gatagccaat agaaaaccgc agagtcaaag ggttttgtat
     9001 gaattgcgag atcttttgaa gagaaatcag tttatactca atgataccaa tccggatatt
     9061 gtcatttcca ttggcgggga tggtatgctc ttgtcggcct ttcataagta cgaaaatcag
     9121 cttgacaagg tccgctttat cggtcttcat actggacatt tgggcttcta tacagattat
     9181 cgtgattttg agttggacaa gctagtgact aatttgcagc tagatactgg ggcaagggtt
     9241 tcttaccctg ttctgaatgt gaaggtcttt cttgaaaatg gtgaagttaa gattttcaga
     9301 gcactcaacg aagccagcat ccgcaggtct gatcgaacca tggtggcaga tattgtaata
     9361 aatggtgttc cctttgaacg ttttcgtgga gacgggctaa cagtttcgac accgactggt
     9421 agtactgcct ataacaagtc tcttggcggt gctgttttac accctaccat tgaagctttg
     9481 caattaacgg aaattgccag ccttaataat cgtgtctatc gaacactggg ctcttccatt
     9541 attgtgccta agaaggataa gattgaactt attccaacaa gaaacgatta tcatactatt
     9601 tcggttgaca atagcgttta ttctttccgt aatattgagc gtattgagta tcaaatcgac
     9661 catcataaga ttcactttgt cgcgactcct agccatacca gtttctggaa ccgtgttaag
     9721 gacgccttta tcggcgaggt ggatgaatga ggtttgaatt tatcgcagat gaacatgtca
     9781 aggttaagac cttcttaaaa aagcacgagg tttctaaggg attgctggcc aagattaagt
     9841 ttcgaggtgg agctattctg gtcaataatc aaccgcaaaa tgcaacgtat ctattggacg
     9901 ttggagacta cgttaccatt gacattcccg ctgagaaagg ctttgaaacc ttggaggcta
     9961 ttgagcttcc attagatatt ctctatgagg atgaccactt tctagtcttg aataaaccct
    10021 atggagtggc ttctattcct agtgtcaatc actctaatac cattgccaat tttatcaagg
    10081 gttactatgt caagcaaaat tatgaaaatc agcaggttca cattgttacc agactagata
    10141 gggatacttc tggcttgatg ctctttgcca agcacggtta tgcccatgca cgattagaca
    10201 agcagttgca gaagaaatct atcgagaaac gctactttgc tttggttaag ggagatggac
    10261 atttggagcc agaaggggaa attattgctc cgattgcgcg tgatgaagat tccattatta
    10321 ccagacgagt ggctaaaggc ggaaagtatg cccatacttc atacaagatt gtagcttctt
    10381 atggaaatat tcacttggtc tatattcacc tgcacactgg tcgaacccat caaatccgag
    10441 tccatttttc tcatatcggt tttcctttgc tgggagatga tttgtatggt ggtagtctgg
    10501 aagatggtat tcaacgtcag gctctgcatt gccattacct atccttttat catccatttt
    10561 tagagcaaga cttgcagtta gaaagtccct tgccggatga tttcagtaac cttattaccc
    10621 agttatcaac taatactcta taaaaactgt ctcagagtat aattattatc ttaaaggaga
    10681 aaactcatgg aagtttttga aagtctcaaa gccaaccttg ttggtaaaaa tgctcgtatc
    10741 gttctccctg aaggggaaga gcctcgtatt cttcaagcaa caaaacgctt agtaaaagaa
    10801 acagaagtga ttcctgtttt gcttggaaat cctgaaaaaa ttaaaattta tcttgaaatt
    10861 gaaggaatca tggatggtta tgaggtcatc gaccctcaac attatcctca atttgaagaa
    10921 atggtttctg ccttggtgga gcgtcgcaag ggcaaaatga ctgaagaaga tgtacgcaag
    10981 gttttggttg aagatgtcaa ctactttggt gtgatgttgg tttacttggg cttggttgat
    11041 ggaatggtgt caggagcgat tcactcaaca gcttcaacag ttcgcccagc tctacaaatc
    11101 atcaaaactc gtccaaatgt aactcgtact tcaggagcct tcctcatggt tcgtggtacg
    11161 gaacgttacc tatttggaga ctgtgccatt aacatcaatc cagatgcaga agccttggct
    11221 gaaattgcca tcaactcagc aatcacagct aagatgtttg gcatcgaacc taaaattgcc
    11281 atgttgagct attctactaa aggttcaggg tttggtgaaa gcgttgataa ggtcgttgaa
    11341 gcaactaaaa ttgctcacga cttgcgtcct gaccttgaaa tcgatggtga gttgcaattt
    11401 gatgcagcct ttgttcctga aactgcagct ctgaaagctc ctggaagtac ggtagctggt
    11461 caagcaaatg tcttcatctt cccaggtatc gaggcaggaa atattggtta caagatggct
    11521 gaacgcctgg gtggctttgc ggctgtagga cctgttttgc aaggtttaaa caagccagtt
    11581 aatgatcttt ctcgtggatg taatgcagat gatgtttaca agttgaccct catcacagca
    11641 gctcaagcag ttcatcaata gtgaaaacta taaagtgata tactatgcta tactgtagtt
    11701 atgaaactat gtacgaaaag cactgccatt aattcctgag aactaaatta ctgattggtg
    11761 tcaaaaagga aaacttccaa gcgatgatat cctgtctata cacgacctat agaaatctgt
    11821 aatatacata tccgtaaaac gataaattcc ctttttgatt ttaaatgagt atgaaaagag
    11881 aattttttgg ctctttgtca actgtagtgg gttgaagaaa agctaagctc gagaaaggac
    11941 aaatttcatc ctttcttttt tgatattcag agcgataaaa atccgttttt tgaagttttc
    12001 aaagttccga aaaccaaagg cattgcgctt gataagtttg atgagattat tg
//
LOCUS       DBDESG010000064        11951 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.064, whole genome shotgun sequence.
ACCESSION   DBDESG010000064 DBDESG010000000
VERSION     DBDESG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.064"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(98..1729)
                     /locus_tag="ACKB38_001868"
     CDS             complement(98..1729)
                     /locus_tag="ACKB38_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGL7980583.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2022..7001
                     /locus_tag="ACKB38_001869"
     CDS             2022..7001
                     /locus_tag="ACKB38_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGL7980584.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7091..8287
                     /gene="pgk"
                     /locus_tag="ACKB38_001870"
     CDS             7091..8287
                     /gene="pgk"
                     /locus_tag="ACKB38_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGL7980585.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADNVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8423..8950
                     /locus_tag="ACKB38_001871"
     CDS             8423..8950
                     /locus_tag="ACKB38_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGL7980586.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9027..9383
                     /gene="glnR"
                     /locus_tag="ACKB38_001872"
     CDS             9027..9383
                     /gene="glnR"
                     /locus_tag="ACKB38_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGL7980587.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9420..10766
                     /gene="glnA"
                     /locus_tag="ACKB38_001873"
     CDS             9420..10766
                     /gene="glnA"
                     /locus_tag="ACKB38_001873"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGL7980588.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10862..10981)
                     /locus_tag="ACKB38_001874"
     CDS             complement(10862..10981)
                     /locus_tag="ACKB38_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGL7980589.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            10946..11095
                     /locus_tag="ACKB38_001875"
     CDS             10946..11095
                     /locus_tag="ACKB38_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGL7980590.1"
                     /translation="MEKFNFKNNIRQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            11239..11376
                     /locus_tag="ACKB38_001876"
     CDS             11239..11376
                     /locus_tag="ACKB38_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980591.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEDLNIRLEVK
                     N"
     gene            11469..11951
                     /locus_tag="ACKB38_001877"
                     /pseudo
     CDS             11469..11951
                     /locus_tag="ACKB38_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3842 a         2181 c         2562 g         3366 t
ORIGIN      
        1 tatatatctg cagaatactc tttcagtttc attcatccta gtcttgctta ggaaaaagta
       61 aaaagccatc caattatgat ggatagcttc ttggttctta gatttgttct gcaaaaacct
      121 tttcagcaag gttcatagcg tggtctgata cacgagtgta gtgtgagatg atgtcgataa
      181 agttgacccc agcttgtgtt gaacattcgc ctttgttgag gcgtttgatg tgggtttttc
      241 tgagaacacg ttctatctta ttgattgctt catgacgttc tacaagactg cgtgcttttt
      301 caatatcatt gttttccaca ctatccagag catctttgat aaagtcacta gtttggcggt
      361 aaacttcctc taattctttc aaggcggcat cagaaaattc aacatttttc cgttgaagat
      421 agtcagtcag attgagtaga gcctccgtgt ggtctccaat ccgttccaaa tcacgggagg
      481 aatcaaggat attggtaagc acttcacttt ctttttggct gagagcttcg cttgaaaggg
      541 caatgagata acgtgttaat tgctcatcga tggtgttaat tgcttcttcg gttttatgcc
      601 ctttttcagc aactttttca tccaagtcaa tgatgtactt ataggaaagg tcaaaggctt
      661 tagcagcgta gtttcctaag tgcaagagct ctttcttagc atttcctaga gcgatagatg
      721 gggcctgttt gatgaaatgt tcatcaagat ataagggttc gtatttgaca acctcgtcct
      781 ctccaggaat aatcttggtt acaaagtaag ccagagctcc gataaatgga aattggacaa
      841 tggtgttggt aatattaaag gttccgtgag caaaggcgat ggtcatttcc ggtgctagat
      901 ttagcgtagc ttcaaaccaa tgaatcagga cagtaaaagg aactagaaaa ataacgcaga
      961 caactgttcc gataacgttg aaggcaacat gagctcctgc tacccgttta gctgcaatat
     1021 tagcccctaa agaggcaatg atggctgtaa tggttgtccc gatattgtca ccaaatagaa
     1081 ctggcaaagc tccctgtaga tcaattagat tgccggcgta gaggttttgt aaaatcccaa
     1141 tggtagccga agaagcttga attagcaagg tcaagccagt accgacaaag acacccaaaa
     1201 caggattctt acttagctca atcatatagt ccttaaagac ctgtaaatcc ttgagtggag
     1261 ccattgcgcc gctcatgaga ttgagggcaa aaaagatacc accgacacca aagaggatgc
     1321 gtccgatatt attgactgtc cgatttttcg taaaaaaaag acagacggca ccgataaaga
     1381 gcataggtag ggcatagtta cctaatttaa aaccgatgag aaaggatgtg acagttgtcc
     1441 caatattagc acccatgaca atcccgatag cctgacgtaa ggttaagaga ccggcactga
     1501 ccaggccgac tgtgataact gttacaccag aactagactg aattagagca gtcatcccaa
     1561 taccaaccag aactccaaag aaaggattac tagtatattt gtcaatgtaa aaacgaaggc
     1621 gatctccagc agcttgttgt aaaccgtctc ccatggtctt gatgctatat aagaatagcc
     1681 ccagaccacc taaaaagtga aataaaattt cctgccaatt aatggacatt tctttttcct
     1741 ccgaaaaata atcgcggaat atctcctatt ctattttaaa ggataaaagt aaatctaaca
     1801 agtgttaatg tcaagtttgt aaaagaaaat tataattgat aagtgtatta tttttaagag
     1861 tttttaacct tattttagat ttgtgcaaac agttattgtt ttttgtctaa aaatgtttta
     1921 ggataaaaat gtgattatca tggttctaga gtttgataaa aaactacaga atataaagcg
     1981 ctttcttgaa aacaacaaaa tcaatctttt aggaggagaa aatgaagaat ccattttttg
     2041 aaagacgttg tcgttacagt attcgtaagt tatcagtagg agcctgctcg ctgatgattg
     2101 gtgctgtttt atttgctggt ccagccttgg ctgaagaaac tgcagttcct gaaaatagcg
     2161 gagctaatac agagcttgtt tcaggagaga gtgagcattc gaccaatgaa gctgataagc
     2221 agaatgaagg ggaacatgct agagaaaaca agctagaaaa ggcagaagga gtagcgatag
     2281 catctgaaac tgcttcgcca gcaagcaatg aagctgcaac tactgaaact gcagaagcag
     2341 ctagcgcagc taaaccagag gaaaaagcaa gtgaggtggt tgcagaaaca ccatctgcag
     2401 aagcaaaacc taagtctgac aaggaaacag aagcaaagcc cgaagcaact aaccaagggg
     2461 atgagtctaa accagcagca gaagctaata agactgaaaa agaagtccag ccagatgtcc
     2521 ctaaaaatac agaaaaaaca ttaaaaccaa aggaaatcaa atttaattct tgggaagaat
     2581 tgttaaaatg ggaaccaggt gctcgtgaag atgatgctat taaccgcgga tctgttgtcc
     2641 tcgcttcacg tcggacaggt catttagtca atgaaaaagc tagcaaggaa gcaaaagttc
     2701 aagccttatc aaacaccaat tctaaagcaa aagaccatgc ttctgttggt ggagaagagt
     2761 tcaaggccta tgcttttgac tattggcaat atctagattc aatggtcttc tgggaaggtc
     2821 tcgtaccaac tcctgacgtt attgatgcag gtcaccgtaa cggggttcct gtatacggta
     2881 cactcttctt caactggtct aatagtattg cagatcaaga aagatttgct gaagctttga
     2941 agcaagacgc agatggtagc ttcccaattg cccgtaaatt ggtagacatg gccaagtatt
     3001 atggctatga tggctatttc atcaaccaag aaacaactgg agatttggtt aaacctcttg
     3061 gagaaaagat gcgccagttt atgctctata gcaaggaata tgctgctaag gtaaaccatc
     3121 caatcaagta ttcttggtac gatgccatga cctataacta tggacgttat catcaagatg
     3181 gtttgggaga atacaactac caattcatgc aaccagaagg agataaggtt ccggcagata
     3241 acttctttgc taactttaac tgggataagg ctaaaaatga ttacactatt gcaactgcca
     3301 actggattgg tcgtaatcct tatgatgtat ttgcaggttt ggaattgcaa cagggtggtt
     3361 cctacaagac aaaggttaag tggaatgaca ttttagacga aaatgggaaa ttgcgccttt
     3421 ctcttggttt atttgcccca gataccatta caagtttagg aaaaactggt gaagattatc
     3481 ataaaaatga agatatcttc tttacaggtt atcaaggaga ccctactggc caaaaaccag
     3541 gtgacaaaga ttggtatggt attgctaacc tagttgcgga ccgtacgcca gcggtaggta
     3601 atacttttac tacttctttt aatacaggtc atggtaaaaa atggttcgta gatggtaagg
     3661 tttctaagga ttctgagtgg aattatcgtt cagtatcagg tgttcttcca acatggcgct
     3721 ggtggcagac ttcaacaggg gaaaaacttc gtgcagaata tgattttaca gatgcctata
     3781 atggcggaaa ttcccttaaa ttctctggtg atgtagccgg taagacagat caggatgtga
     3841 gactttattc tactaagtta gaagtaactg agaagaccaa acttcgtgtt gcccacaagg
     3901 gaggaaaagg ttctaaagtt tatatggcat tctctacaac tccagactac aaattcgatg
     3961 atgcagatgc atggaaagag ctaacccttt ctgacaactg gacaaatgaa gaatttgatc
     4021 ttagctcact agcgggtaaa accatctatg cagtcaaact atttttcgag catgaaggtg
     4081 ctgtaaaaga ttatcagttt aacctaggac aattaactat ctcggacaat caccaagagc
     4141 cacaatcgcc gacaagcttt tctgtagtga aacaatctct taaaaatgcc caagaagcgg
     4201 aagcagttgt gcaatttaaa ggcaacaagg atgcagattt ctatgaagtt tatgaaaaag
     4261 atggagacag ctggaaatta ctaactggct catcttctac aactatttat ctaccaaaag
     4321 ttagccgctc agcaagtgct cagggtacaa ctcaagaact gaaggttgta gcagtcggta
     4381 aaaatggagt tcgttcagaa gctgcaacca caacctttga ttggggtatg actgtaaaag
     4441 ataccagcct accaaaacca ctagctgaaa atatcgttcc aggtgcaaca gttattgata
     4501 gtactttccc taagactgaa ggtggagaag gtattgaagg tatgttgaac ggtaccatta
     4561 ctagcttgtc agataaatgg tcttcagctc agttgagtgg tagtgtggat attcgtttga
     4621 ccaagccacg taccgttgtt agatgggtca tggatcatgc aggagctggt ggtgagtctg
     4681 ttaacgatgg cttgatgaac actaaagact ttgaccttta ttataaagat gcagatggtg
     4741 agtggaagct agctaaggaa gtccgtggta acaaagcaca cgtgacagat atcactcttg
     4801 ataaaccaat cactgctcaa gactggcgct tgaatgttgt cacttctgac aatggaactc
     4861 catggaaggc tattcgtatc tataactgga aaatgtatga aaagcttgat actgagagtg
     4921 tcaatattcc gatggccaag gctgcagccc gttctctagg caataacaag gtacaagttg
     4981 gctttgcaga tgtaccggct ggagcaacta ttaccgttta tgataatcca aattctcaaa
     5041 ctccgctcgc aaccttgaag agcgaagttg gaggagacct agcaagtgca ccattggatt
     5101 tgacaaatca atctggtctt ctttattatc gtacccagtt gccaggcaag gaaattagta
     5161 atgtcctagc agtttccgtt ccaaaagatg acagaagaat caagtcagtc agcctagaaa
     5221 caggacctaa gaaaacaagc tacgccgaag gggaggattt ggaccttaga ggtggtgttc
     5281 ttcgagttca gtatgaagga ggaactgagg acgaactcat tcgcctaact cacgcaggtg
     5341 tatcagtatc aggttttgat acgcatcata agggagaaca gaatcttact ctccaatatt
     5401 tgggacaacc ggtaaatgct aatttgtcag tgactgtcac tggccaagac gaagcaagtc
     5461 cgaaaactat tttgggaatt gaagtaagtc aggaaccgaa aaaagattac ctagttggtg
     5521 atagcttaga cttgtctgaa ggacgctttg cagtggctta tagcaatgac accatggaag
     5581 aacattcctt tactgatgag ggagttgaaa tttctggtta cgatgctcaa aagactggtc
     5641 gtcaaacctt gacgcttcat taccaaggcc atgaagttag ctttgatgtt ttggtatctc
     5701 caaaagcagc attgaacgat gagtacctca aacaaaaatt agcagaagtt gaagctgcta
     5761 agaacaaggt ggtctataac tttgcttcat cagaagtaaa agaagccttc ttgaaagcaa
     5821 ttgaagcggc cgaacaagtg ttgaaagacc atgaaactag cacccaagat caagtcaatg
     5881 accgacttaa taaattgaca gaagctcata aagctctgaa tggtcaagag aaatttacgg
     5941 aagaaaagac agagcttgat cgcttaacag gtgaggttca agaactcttg gctgccaaac
     6001 caaaccatcc ttcaggttct gccctagctc cgcttcttga gaaaaacaag gccttggttg
     6061 aaaaagtaga tttgagtcca gaagagctta caacagcgaa acagagtcta aaagatctgg
     6121 ttgctttatt gaaagaagac aagccagcag tcttttctga tagtaaaaca ggtgttgaag
     6181 tacacttctc aaataaagag aagactgtca tcaagggttt gaaagtagag cgtgttcaag
     6241 caagtgctga agagaagaaa tactttgctg gagaagatgc tcatgtcttt gaaatagaag
     6301 gtttggatga aaaaggtcaa gatgttgatc tctcttatgc ttctattgtg aaaatcccaa
     6361 ttgaaaaaga taagaaagtt aagaaagtat ttttcttacc tgaaggcaaa gaggcagtag
     6421 aattggcttt tgaacaaacg gatagtcatg ttatctttac agcacctcac tttactcatt
     6481 atgcctttgt ttatgaatct gctgaaaaac cacaacctgc taaaccagca ccacaaaaca
     6541 cagtccttcc aaaacctact tatcaaccga cttctgatca acaaaaggct cctaaattgg
     6601 aagttcaaga ggaaaaggtt gcctttcatc gtcaagagca tgaaaatact gagatgctag
     6661 ttggggaaca acgagtcatc atacagggac gagatggact gttaagacat gtctttgaag
     6721 ttgatgaaaa cggtcagcgt cgtcttcgtt caacagaagt catccaagaa gcgattccag
     6781 aaattgttga aattggaaca aaagtaaaaa cagtaccagc agtagtagct acacaggaaa
     6841 aaccagctca aaatacagca gttaaatcag aagaagcaag caaacaattg ccaaatacag
     6901 gaacagctga tgctaatgaa gccctaatag caggcttagc cagccttggt cttgctagtt
     6961 tagccttgac cttgagacgg aaaagagaag ataaagatta aatatcgaaa aatcttgtga
     7021 aatctttcct tatatttcca aagtgtgata taatagtttc gaataaaata aataaaggag
     7081 tttttgtaac atggcaaaac ttactgttaa agacgttgac ttgaaaggta aaaaagtcct
     7141 cgttcgtgtt gacttcaacg taccattgaa agatggcgta atcactaacg ataaccgtat
     7201 cacagcagct cttccaacta ttaagtacat catcgaacaa ggtggacgtg caattctttt
     7261 ctctcacctt ggacgtgtga aagaagaagc tgataaagct ggtaaatcac ttgctcctgt
     7321 agcagctgac ttggcagcaa aacttggtca agatgttgtt ttcccaggtg tcactcgtgg
     7381 tgctgaatta gaagcggcaa tcaacgctct tgaagatgga caagttctct tggttgaaaa
     7441 cactcgttac gaagatgttg acggcaagaa agaatctaaa aacgatcctg aacttggtaa
     7501 atactgggca tcacttggag atggtatctt cgtaaacgat gcattcggta cagctcaccg
     7561 tgcacacgca tctaacgttg gtatctcagc aaacgttgaa aaagcagttg ctggtttcct
     7621 tcttgaaaac gaaattgcct acatccaaga agcagttgaa actccagaac gtccattcgt
     7681 ggctatcctt ggtggttcaa aagtttcaga caagatcggt gttatcgaaa acttgcttga
     7741 aaaagctgat aacgtcctta tcggtggtgg gatgacttac acattctaca aagcacaagg
     7801 tatcgaaatc ggtaactcac ttgtagaaga agacaaattg gatgttgcga aagctcttct
     7861 tgaaaaagca aatggtaaat tgatcttgcc agttgactca aaagaagcta acgcatttgc
     7921 tggttacact gaagtgcgtg acactgaagg tgaagcagtt tctgaaggct tccttggtct
     7981 tgacatcggt ccaaaatcta tcgccaaatt tgacgaagct ttgactggtg ccaaaacagt
     8041 cgtatggaac ggacctatgg gtgtatttga aaacccagat ttccaagctg gtacaatcgg
     8101 tgtgatggac gctatcgtga aacaaccagg agttaaatca atcatcggtg gtggtgactc
     8161 agctgccgca gcgattaacc ttggccgtgc agacaagttc tcatggatta gtacgggtgg
     8221 tggagcatca atggaacttc ttgaaggtaa ggttcttcca ggacttgcag ccttgacaga
     8281 aaaataagat tttataaata aatcaaagaa gagagggatg aaagttcctc ttttcttttg
     8341 cttaaaataa aaacgcttcc tctcaactat tactcataaa aatcaccgat ttatgataaa
     8401 atggaaatag aaagttgaga ttatgagtta ttttaaaaaa tataaattcg ataaatccca
     8461 gttcaaactt ggtatgcgaa cctttaaaac aggtattgct gtttttctag ttctcttgat
     8521 ttttggcttt tttggctgga aaggtcttca aattggtgct ttgacagccg tttttagcct
     8581 gagggagagt tttgatgaga gtgttcattt tgggacttcg cgtattctag gaaatagtat
     8641 cggtggactc tatgccttgg tcttcttctt attaaatacc tttttccacg aagccttttg
     8701 ggtgaccttg gtagttgttc caatctgcac catgttaacc attatgacaa atgtagccat
     8761 gaataacaaa gcaggggtta ttggtggtgt agcagctatg ttaatcatta ccctatcaat
     8821 tccaagtggt gagacaattt tgtacgtgtt tgtgcgtgta ttagaaacgt ttatgggagt
     8881 ttttgtcgca attatcgtaa attacgatat tgatcgtatt cgtctctttt tagagaaaaa
     8941 agaaaaataa tgttacattt tataacatta tcaattgacg tttgtctttt tttagactat
     9001 aatagacaga aagaaggaaa ttgtaaatga aggaaaaaga atttcgccga aatatggctg
     9061 tttttcctat cggcagtgtt atgaagttga ccgatctatc ggcgcgtcag attcgttatt
     9121 atgaagatca agagttgatc aagcccgatc gaaacgaagg aaatcgtcgc atgtattcct
     9181 tgaatgacat ggatcgtctg cttgaaatca aagattatat ctctgaaggt tataatatcg
     9241 ctgccattaa gaaaaaatat gctgaacgtg aagcgaaatc caagaaagcg gttagtcaga
     9301 ctgaagtacg tcgtgcactt cacaatgaac tcctccaaca ggggcgtttt gcttcagtac
     9361 agtcaccttt tggtcgcggt taggcaaccg caagtagtca tagattaagg agaaaactta
     9421 tgccaatcac agctgcagat attcgtcgtg aagtcaagga aaaaaatgtt acctttattc
     9481 gtcttatgtt ctcagatatt ttgggaacca tgaaaaacgt cgaaattcct gctacagatg
     9541 aacagttaga taaggtcttg tcgaacaagg ttatgtttga tggatcttct attgaaggtt
     9601 ttgtacgtat caatgagtcg gatatgtact tgtacccgga cttggataca tggacagtct
     9661 tcccttgggg agatgaaaat ggaagtgttg caggtctgat ctgtgatgtc tatacaacag
     9721 aaggtgaacc atttgcgggt gaccctcgtg gtaatttgaa acgagctctt cgtcacatgg
     9781 aagaagttgg attcaaatcc ttcaaccttg gtccagagcc agaattcttc ctatttaagt
     9841 tggatgaaaa tggggaccca acacttgaag tgaatgacaa gggtggctac tttgacttgg
     9901 cacctactga ccttgcggac aacacacgtc gtgagattgt gaatgtcttg accaaaatgg
     9961 gatttgaagt agaagcgagt caccacgagg ttgcggttgg acagcatgag attgacttta
    10021 agtacgatga agttctccgt gcttgtgata agattcaaat ctttaagctt gttgttaaaa
    10081 ccattgctcg caaacacgga ctttacgcaa catttatggc gaagccaaaa tttggtattg
    10141 ctggatcagg tatgcactgt aatatgtcct tgtttgatgc agaaggaaat aacgccttct
    10201 ttgatccaaa tgatccaaaa ggaatgcagt tgtcagaaac agcttaccat ttcctaggcg
    10261 gtttgatcaa gcatgcttac aactatactg ccatcatgaa cccaacagtt aactcataca
    10321 aacgtttggt tccaggttat gaagcgcctg tttacattgc ttgggctggt cgtaaccgtt
    10381 cgccacttgt gcgcgtacct gcttcacgtg gtatgggaac tcgtcttgag ttgcgttcag
    10441 tggatccaat ggcgaaccct tacgttgcta tggctgttct tttggaagtt ggtttgtatg
    10501 gtattgaaaa taaaatcgaa gcaccagctc ctatcgaaga aaatatctac atcatgacag
    10561 cagaagagcg caaggaagct ggtattacag accttccatc aactcttcac aacgctttga
    10621 aagctttgac agaagatgaa gtggttaaag ctgctctcgg agatcacatc tatactagct
    10681 tccttgaagc caaacgaatc gaatgggcaa gttatgcaac cttcgtttca caatgggaaa
    10741 ttgataatta tttagacctt tactaatact aatatagaag aaagattgcc tgtgggtaaa
    10801 cagtcacaca gcagtctaag ccatcaaata cctataaccc ttaaaatagc gatttcggtc
    10861 gctatttatt tttcgaaaaa tcctcttgac tcttttgcga attagattta tcatcagacg
    10921 tgtagaagaa aattaaggag tcactatgga aaaattcaat ttcaaaaaca atatcaggca
    10981 agaaaacaag ttacttcaaa tcgaaatcta caagtttact aacttttgca agcttcaaaa
    11041 ttacacatct gtaaatattt tttcgaaaga tatatttgaa gccatagtaa attgagacta
    11101 gaatagtaca ctacatctgc taaaaatttc tagaaactaa tttaactttc ctaatcgatt
    11161 tgtttacatc ttatttcaat ctactatatt atctcaataa atgatgatgg cgactgtttt
    11221 gtttgtgctt gtcgattttt gaatcctgat cgagcggaag aatatttctg ccgaggttgc
    11281 caaggggaaa atcctgaaga tattgaattt tatgatgaac aattacaggc agaaaaggtg
    11341 gaggatttga atattcgatt ggaagtaaaa aattgaatta tctacaatct catcaacaat
    11401 aaacctattt caaccccatt atctataggc gtatttttac gcctttttat atacttgaag
    11461 cacataagaa acattatggg aagtttgctg atggaagcac tcaagaaatt gatgttcctt
    11521 atgatattcc tgatacttgg gagtgggtga ggataaaatc aatttattgg aattttgggc
    11581 aaaataagcc agagaaatcc tttaggtata tagatacgtc tagtattgat agaaaaaaga
    11641 acataatcaa ctacaaaaat ctacaatatc tttcacctga acaagcgcct tcccgtgcta
    11701 gaaaattagt ttcgcagaat agtgtcttat tttcaacagt tagaccatat ctaaaaaata
    11761 ttgctgtagt tagagaactt aaagagtatt tgatagctag tacagcattt attgttttgg
    11821 atactttact taacgaaaca tatttgaaat attatttatt atcagataat tttatcaatc
    11881 gtgttaataa taaatctact ggaacaagtt atcctgcaat caatgattat aattttaatc
    11941 tattattaat t
//
LOCUS       DBDESG010000065        11230 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.065, whole genome shotgun sequence.
ACCESSION   DBDESG010000065 DBDESG010000000
VERSION     DBDESG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.065"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..958
                     /locus_tag="ACKB38_001878"
                     /pseudo
     CDS             1..958
                     /locus_tag="ACKB38_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611511.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1083..1331)
                     /locus_tag="ACKB38_001879"
     CDS             complement(1083..1331)
                     /locus_tag="ACKB38_001879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980592.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(1279..2361)
                     /locus_tag="ACKB38_001880"
     CDS             complement(1279..2361)
                     /locus_tag="ACKB38_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGL7980593.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFNLFTSTSKDLLYFD"
     gene            complement(2487..3269)
                     /gene="gatD"
                     /locus_tag="ACKB38_001881"
     CDS             complement(2487..3269)
                     /gene="gatD"
                     /locus_tag="ACKB38_001881"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGL7980594.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3269..4612)
                     /gene="murT"
                     /locus_tag="ACKB38_001882"
     CDS             complement(3269..4612)
                     /gene="murT"
                     /locus_tag="ACKB38_001882"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGL7980595.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4711..6027)
                     /locus_tag="ACKB38_001883"
     CDS             complement(4711..6027)
                     /locus_tag="ACKB38_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGL7980596.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6220..7446
                     /locus_tag="ACKB38_001884"
     CDS             6220..7446
                     /locus_tag="ACKB38_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGL7980597.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            7645..9219
                     /locus_tag="ACKB38_001885"
     CDS             7645..9219
                     /locus_tag="ACKB38_001885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGL7980598.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9483..10271
                     /gene="codY"
                     /locus_tag="ACKB38_001886"
     CDS             9483..10271
                     /gene="codY"
                     /locus_tag="ACKB38_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGL7980599.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PIESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10271..10846
                     /locus_tag="ACKB38_001887"
     CDS             10271..10846
                     /locus_tag="ACKB38_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGL7980600.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            10928..11013
                     /locus_tag="ACKB38_001888"
     tRNA            10928..11013
                     /locus_tag="ACKB38_001888"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10962..10964,aa:Leu,seq:aag)
BASE COUNT         3326 a         2107 c         2467 g         3330 t
ORIGIN      
        1 tattgttgag aaaacaagag ggagagtacc tgagagcgga gaatgccatc ctaaaaaagt
       61 taagagaact ccgattgaag gaggaaaaag agaaagaaga aagacagaaa ttgttcaaga
      121 attaatgact gagttttcgt tagatcttct tctagaagcc attaaactag ctcgttggac
      181 ctactactat cacttgaaac agctagacaa aacagataaa gaccaagagc ttaaaactga
      241 aattcaatcc atctttatcg aacacaaggg aaattatgct tatcgccggg ttcatttaga
      301 actaagaaat cgtggttatc tggtaaatca taaaagagtt caaggcttga tgaaagtact
      361 caatttacaa gctaaaatgc gaaagaaacg aaaatattct tctcataaag gagacgttgg
      421 taagaaggca gagaatctca ttcaagccca atttgaaggc tctaaaacaa tggaaaagtg
      481 ctacacagat gtgactgaat ttgccattcc agcaagtact caaaagcttt acttatcacc
      541 agttttagat ggctttaaca gcgaaattat tgcttttaat ctttcttgtt cgcctaattt
      601 agaacaagta caaaccatgt tggaacaggc attcacagag aagcactacg agaatacgat
      661 tctccatagt gaccaaggct ggcaatacca acacgattct tatcatcggt tcctagagaa
      721 taagggaatt caagcatcta tgtcacgcaa gggaaacagc caagacaacg gtatgatgga
      781 atctttcttt ggcattttaa aatccgaaat gttttatggc tatgagaaaa catttaaatc
      841 acttaaccaa ttggaacaag tcattataga ctatattgat tactacaaca acaaacgaat
      901 taaggtaaaa ctaaaaggac ttagtcctgt gcagtacaga actaaatcct tcggataaat
      961 taattgtcta actgtttggg gtcagtacac aatacctgat actatgcgtt ttattgtggg
     1021 gagattttcc tcattttcta gtgaaattga actttttcta acatcttttg atagatgact
     1081 tcttaatcaa ttatcatttc cattgttgtt tcttggggtt tcatcccttg tctttgataa
     1141 aagcggagag caccctcgtt atcattccag acacttaggg ttagattatt gcagccaatt
     1201 tctttagcat aatagcagaa aaatgacaat aaaatagccc ctagatattt tgaaaggaaa
     1261 gcataggggc tatatagttt aatcaaaata aagcaaatct ttgctggtgc ttgtaaagag
     1321 gttgaagcca tccttggtaa caacaccgca gtcttcaata cgaacaccga ctttaccagg
     1381 gatatagata cctggttcaa cagagaagca catgccttct tcgatgacca tgtcgtttcc
     1441 ttccatgata gatgggaatt catggacatc cataccgata ccatgcccga gacggtggtt
     1501 gaagtactca ccataaccag ctttttcgat gacctcacgg gcagcgcggt ccacttcatg
     1561 agcagtcaca cctggcttga taaagtcaag agcagcttgt tgggcttcaa gagtcaagtt
     1621 gtaaatatct ttcttgaatt ggtctggttt gccgacagcg actgtacgag tcatatctga
     1681 cgcatagcca ttgaccagaa cacccaggtc aaagaggaga agagcatcat tttcaacctt
     1741 attagctgct ggaatgccgt gtggattcgc agcattatca ccagtcaaga ccatggtatc
     1801 aaagctcatt tcataacctt cacgtttcat ggcaaagtcg atttgtgcga tgatatctgt
     1861 ctcagtctta tcaagagaaa tattgtcaaa accaacatga acagccttgt cagcataaag
     1921 acctgcaacc atcatttttt gcacttcatc agctgatttg atgaggcgca tgcgttggat
     1981 acgaggagtg aggttgtcaa actcagcagt ctcaaaaact gttttcaaac catggtattt
     2041 ggtcaagatg agattgtcaa actcaacagc gacacgtttg aagtcaagtt gtggaagagc
     2101 atgtttgatt ttttgccatg gattttcaga atcgacatag cccactactg ggaaggaaac
     2161 ggtgctactt gcacgttcta cttcaagagc tgggacaaag aggagaggtt cctgatctgc
     2221 taggacaaag aggaacattt ggcgttcatg gggatcactg taaaaaccag tgaggtaatt
     2281 gattgtgacg gggtcagata cgacagcgac gtctagtttt tctgattcaa gatatgttag
     2341 gatttgttgt aatttagaca tatgctacct tctttctacc cctctatttt ggcaaaaaat
     2401 gagagaaaat gcaagggaga agggtaaaag aaaagacaaa aaactccgac aagataacgg
     2461 agttctttga tatcattttc ctttgtttaa gaaaagtcag ccttgctttt gacgtcactg
     2521 tactcttcag cgatttcttg gctgagaatg tcctcatagg cagggagttg gatgtcctga
     2581 ccatatttct tcttgagggc agtagtaact aggcgataag ccagattggc attacgagag
     2641 aggataggcc cgtggaagta ggaaccaaag acattcttat aatgaacccc ttcaccgacc
     2701 ttttcttcgt tgtttccatt tccatagaca acctgtccca gcggtttttg gtcatcagag
     2761 aggaaggtac gaccttggtg attttcaaat ccatagtagg tttcatcgaa atcttcattg
     2821 tgaatcttga tgtcaccgat aaaacggtta ttggtctggt tgagcgtgta gtgtcccatg
     2881 acccctagcc cttcgatacg ttttcctgaa gcttcaacat aatattgacc caatagttgg
     2941 aaaccaccgc agatagccag aactacaccg tcgttttgga tgtagttgtc aatgctctct
     3001 tttttagcag gtaggtcgtc tgcaatgata ctttgttcaa agtcttgacc accaccgaaa
     3061 aaggcgatgt cgtagtgatt ttcatcaaag tcatcatgga gagaaacgat gtcaacggtc
     3121 acatgggctc ccagtttttc agccacatac ttgagcatga ggatgtttcc attgtccccg
     3181 taggtattca tgagatttcc gtagaggtgg gcaatgttga gctgataggg gtaattgcca
     3241 tcttttgagg aaagtgaagt ataaaccatt agttcatctc ctttctaaca atctgacgac
     3301 tagccagcag ttcacgaaat tccagcatgg cagtataagt tgccagaata taggcatgct
     3361 tgcagtcttg attctcaatg gtcttgagaa cttgctccag attactcgtt tcagtgattt
     3421 tctcagctgg atagccagtc actcggaggc gacgagcgat ttcagaatga cgaacaccgc
     3481 cagcgttgat ttcaggaatg tccatgtcag tgatttgttc aaagtctgca tcccagatcc
     3541 agctagtgtc aattccatct gcatagttgg cattaaggag gacagatagg ctaaatggat
     3601 aaggtgctag tttgatcatt tcgatagctt gggttgcacc gactggattt ttaatcaaga
     3661 caagggtaca ttccttgtca ccgatatgaa aggtttcttg gcgtccaaag acagcacggc
     3721 tcttgtcaaa tccctgtttg atgagttgcg aatcggcacc taggaaacgg gcgatggcca
     3781 cagcagctag ggcgttatag atattataga gcccgccgat ttggataccg tattcttggc
     3841 cgtctatgac aaagcgagag cgattgttgg tcaactcaac cagttttgtc aaacgatagt
     3901 cgagatcagg acgtttacat ccacaacctt cacagatata ggcacccaag tttgcatagg
     3961 tattatgctc atatttgagg atgccttggc agtcaggaca gagaatccct tcggtattgt
     4021 agtgagccag ttgggctggt cccttttcca agtcaaaacc aaaatactct atagggtttg
     4081 gaatagttgg cttgtagaaa agtggactgt ctccgttaag gagaacagta gcagttggaa
     4141 ctttccgaat ggcatccaat atcatgttat aggtagtata gatttcaccg aaacggtcca
     4201 tctggtcacg gaagatatta gtaatgacaa aaagactagg ctggatatag tcacagatac
     4261 gagatagact ggcttcgtca atttcgagga cggcaatatt tttcccagtt ttagaagatt
     4321 tggctgttag gaaggttgtt gcaatccctg taatcatgtt ggcaccgctt gggttggtta
     4381 gaacttgacc ataaacctct tttaaaatgc cgacagtgag ggcagttgtc agggtttttc
     4441 catttgttcc agtgacaacg acaatctcgt agttcttagc taggttttgt aaaatatctt
     4501 tatcaaattg aagggcgact ttccctggga gcgtacttcc acgtccaaga cggcttaaaa
     4561 cgaagtggga agaacgccca gcaagaaggc ccaaagtagt ttttaagttc atgtttctat
     4621 tatatcataa aagaggagaa agacagattt gagattccgc agaagtaaaa acagcccaaa
     4681 gagggctgtt cgatacgatt aaatctcaac ttaaatcgca aacaagtcat tcaagttctc
     4741 agccaaggtt gggtgagtga agatttgttt tgtgaagtaa gtgtaaggaa tcttgttgtc
     4801 catagcaaca gtgatgatgt tgatgatttc ttgagaacct tctgagaaga tgcttgctcc
     4861 aagaatttct tttgtttcag tattgacaac agctttgaag gcaccgcgaa ggtctccatt
     4921 tacgtgacca cgaggcattg ctgcaacggg gatttcctta acagcgtatg gaagtttcaa
     4981 atcagctgct tggctttcag tcaaaccaac ttgtgaaagt gcaggtgtga tgaacatagt
     5041 atttggcaca ttgagacggt cttcaagtgt atagctgcca tctccagcaa ggtagctgta
     5101 aacaacacgg aagtcatcaa gtgaaatgta agtaaattga aggccaccgt tgacatctcc
     5161 aactgcaaag acaccaggaa cgtttgtttg acagtgtttg tctactttaa tagcaccacg
     5221 ttcagttagt tcaatatctg tattttcaag ttgaagtggt tctacatttg gtttgcgtcc
     5281 agttgcgtag agaagggcgt cgaaacggta agtttcgtct tcagttacga caagcacttg
     5341 gtcaccatcg tttttgattt cagtagtatg gatattttga agcaattcaa tgccatcttc
     5401 ttccatgtat tgtttagcaa gagctgcgat ggaaggttct gcacgaggta ggaatgtatc
     5461 caaggcatct aggactgtga ccttgcttcc aagtttgttg taaaggccgg caaattcaag
     5521 accgatattt ccgccaccaa ggattccaag tttttcaggt aatttgtcca agctttggat
     5581 acctgttgag tcaaagatgt ttttgcttgt agcaagtcca gggattggca agacgtttga
     5641 aacagcacca gtgttgatga cgattgtttc agcagtcagt tctttctttt catcaccagc
     5701 ttggatttcg atgactttat ttgaaaggaa gtgagcttcc gcatcaaaga tatctacgcc
     5761 tgtaccagca acagtcgcat agtttttacc gttgaggcga ccagtgatcg tgtttttagt
     5821 agcaatgact tcttcaaaag acaagtcctt ttcagcagca actagcaagg ttttagttgg
     5881 gatacaacca atgttgatac aagttccacc gtacatagcc ttgctgcgtt caacgagggc
     5941 aacttttttg ccagctgaag ccaatttacc tgctagtgtt ttaccagctt taccaaatcc
     6001 aataacaatt aaatcgtatg ttaacattta tcttcctcct attcgaattt cattctagca
     6061 caagaaaaaa acttttgcac aattctgcta cggaaaagac agaaaaagat aaaaacgctt
     6121 tccagacaaa aaactagtaa ttttttgaca agcttgataa actaaaatcc tatctatttt
     6181 tatataagat agaataaatg ttcgaaaata ggaggtttta tgaaatcgaa tcgttatatt
     6241 attgcctttg ctggggtcat tttacactta atgctgggtt cgacttatgc ttggagtgtt
     6301 tatcgtaacc ctattattga aaaaacggga tgggatcagg cttctgttgc cttcgccttt
     6361 agtctagcaa tcttttgttt gggcttatcg gctgcattta tggggcgttt ggtagaaaaa
     6421 tttggtccga aagtcatggg aagtctatct gcttttctat acgcaggtgg aaatatctta
     6481 acaggatttg caatagaccg tcaggagctg tggttgttgt atctcgctta tggcatttta
     6541 ggtgggcttg gtttgggagc aggctatatt acccctgtgt cgacgattat aaaatggttt
     6601 cctgataaac gtggtctcgc aacaggttta gcgattatgg ggtttggttt tgcttcttta
     6661 ttgactagtc ccatagcgca acacctcatc gcaggggtag ggcttgtaga aactttttat
     6721 attttaggag caagttactt tattatcatg ctcctagctt cacaattcat taagcgtcca
     6781 aatgagcaag agcttgcaat tttatcttct tcagggaaag aaaaaacagc ctctttgacg
     6841 caaggaatgg ctgcaaatca ggctctaaaa agcaatcggt tttatatgct ttggattatt
     6901 ttctttatca acatagcttg tggtttaggc ttaatttcag cggcatcgcc aatggcacag
     6961 gagatggctg gcttgtctac aagtcatgca gcagtaatgg tgggtgtttt ggggattttc
     7021 aatggatttg gtcgcttgct ctgggcgagt ttgtctgact atatcggtcg ccctctaacc
     7081 tttagtatat tactgcttgt caatcttttc ttttctctct cactttggct ctttacagat
     7141 tccgttttat ttgtagttgc tatgtctatt ttgatgactt gctatggagc tggtttttct
     7201 ttgattccag cttatctcag tgatattttt ggaaccaagg aattggccgc tctgcatggt
     7261 tatattttaa cagcttgggc aatggctggt ttagcgggac ctattttatt agcagagact
     7321 tataaaatgg ctcattcgta cacacaaacc ttgttcgttt ttctcatttt atacagtatc
     7381 gccttggctt tgtcttatta tctaggtcgt tcaatcaaaa aagaaagtca aaaagcgctt
     7441 acatgatttt ctgtagaaat ggggctatct tttgctctgg tcttgtgtta tactagatag
     7501 gttgcaaaga aaacagtact tttcttttgt ggaaaagaag caacacgatt ttatatctag
     7561 ttatatgaaa atacgtcata aaaagaaaag tataaactaa gccctatagg gtggcttcgc
     7621 accaccttta gaaagaagaa taacgtgaaa tttaatgaat taaacttgtc tgctgatttg
     7681 ctagcagaaa ttgaaaaagc tggttttgta gaagctagtc ctatccaaga acaaactatt
     7741 cctttggccc ttgaaggcaa ggatgttatc ggtcaagctc agactggtac aggaaaaact
     7801 gcagcctttg gcttgcctac ccttgaaaaa atccgtacag aagaagcgac tatccaagcc
     7861 ttggtcatcg ctccaactcg tgaactagct gtccaaagtc aagaagaact cttccgcttt
     7921 ggtcgtagta agggagtcaa agtccgttca gtatatggcg gatcaagcat tgaaaaacaa
     7981 attaaggctc ttaaatctgg tgcccatatt gtggtgggaa ctccaggtcg cctcttggac
     8041 ttgattaaac gcaaggcctt gaaattacaa gacattgaaa cccttatcct tgacgaagcg
     8101 gatgaaatgc ttaacatggg cttccttgaa gacatcgaag ccattatttc ccgtgtacct
     8161 gagaaccgtc aaactttgct tttctcagca actatgccag atgccatcaa acgtatcggt
     8221 gttcagttta tgaaagcccc tgaacatgtc aagattgcgg ctaaggaatt gacaacagaa
     8281 ttggttgacc agtactatat ccgtgttaag gaacaagaaa aatttgacac catgactcgt
     8341 ctcatggatg tggcacaacc agaactcgct attgtatttg gtcgtaccaa acgccgtgtg
     8401 gatgaattga ctcgtggttt gaaaattcgt ggcttccgtg cagaaggaat tcatggcgac
     8461 ctagaccaaa acaaacgtct tcgtgtcctt cgtgacttta aaaatggcaa tcttgatgtt
     8521 ttggttgcga cagacgttgc agcgcgtggt ttggatattt caggtgtgac ccatgtctac
     8581 aactacgata ttccacaaga tcctgagagt tatgttcacc gtatcggtcg tacaggtcgt
     8641 gctggtaagt caggtcaatc tattactttt gttgctccaa acgaaatggg ttaccttcaa
     8701 atcattgaaa acttgactaa gaaacgcatg aaaggtctca aacctgcaag tgtagaagaa
     8761 tccttccaat caaaaaaaca ggtagctctc aagaaaatcg aacgtgattt tgcagatgaa
     8821 accattcgtg ccaactttga gaaatttggt aaggatgctc gcaaattggc tgctgagttt
     8881 actccagaag aattggcaat gtatatcttg agtctgacag tccaagaccc agatagcctt
     8941 ccagaagtgg agattgcacg tgaaaaacca ctaccgttta aaccatcagg taatggtttc
     9001 ggtggtaaag ctaagggagg tcgtggaggc cgtcgtgggg acgaccgtcg agagcgtgat
     9061 cgccgtggca atggtcgccg tgatgagttc aaaaaaggaa gtcgtggcaa cgatcgtttt
     9121 gataaggaaa aacgttaccg taaggataat aaaaaaccac gcaatacttt aagcgaaaag
     9181 caaacaggct ttgttattcg taacaaaggc gataaataag aaaaagcggt tcaaaatgaa
     9241 tcgctttttt atataaggag accgaatgtg aaaagacaaa taaaattatt atattatctt
     9301 ttataatatt atgatacaca aaaataggca acttgtcaat agaaaaggaa caatttaatt
     9361 aaaaatattc tggtttttaa ttaaattgta agtcaatgca agtcaataag gaattttcag
     9421 ataattattg aaaaagagac gtttttatgt tataatgata gcgattaaat tcgaggtgaa
     9481 aaatggcaca tttattagaa aaaactagaa aaattacatc aattttgaaa cgctcagagg
     9541 agcagttgca ggatgagctc ccttacaacg ctattacgcg tcagttagcg gatattattc
     9601 attgcaatgc ctgcattatc aatagtaagg gacgtctgct tggctatttt atgcgttata
     9661 aaacaaatac agatcgcgta gagcaattct tccaaactaa gattttccca gatgactacg
     9721 ttcaaggggc taatatgatt tacgaaacag aagcaaactt gcctgttgag catgatatga
     9781 gtattttccc tattgagagt agagatgatt ttccagatgg cttgacgact attgcaccga
     9841 ttcatgtatc ggggattcgc cttggttctt tgattatttg gcgtaatgat aaaaaattcg
     9901 aagatgagga cttggttctt gttgagattg ccagtaccgt tgttgggatt cagcttctta
     9961 acttccaacg tgaagaagat gagaaaaata ttcgtcgtcg tactgctgtc accatggcgg
    10021 tcaataccct ttcttactcc gaactccgtg ctgtttcagc aattttaggg gaattaaatg
    10081 gaaatgaagg gaagttgact gcgtcagtga ttgcagatcg tatcggaatc actcgctctg
    10141 tgattgtcaa tgctcttcgt aaacttgagt ctgcggggat tattgaaagt cgctcacttg
    10201 gaatgaaggg aacctatctt aaggtcttga tttcagatat ttttgaagaa gtgaagaaaa
    10261 gagattacta atgacaaagg ctttaatttc gattgattat acagaagatt ttgttgctga
    10321 tagtgggaaa ctaacagcag gtgctccagc tcaggcgatt tcggatgcca tcagcaaggt
    10381 aactcgatta gcttttgaac gaggagatta tatcttcttt actattgatg ctcatgaaga
    10441 aaacgattgt ttccatccgg aaagtaagtt atttcctcct cataatctga ttgggacgag
    10501 tggacggaat ttatatggag atttggggat cttttatcaa gagcatggtt cagacagtcg
    10561 tgtcttttgg atggataaac gccattactc agctttttca gggactgacc tagatattcg
    10621 tttgagagag cgtagagtgt ctaccgttat cttaacaggt gtcttgacgg atatctgtgt
    10681 cctacataca gctatagatg cctataatct aggatatgac atcgagattg ttaaaccagc
    10741 tgttgcttcc atctggcctg aaaatcatca atttgcccta ggtcatttca aaaatacact
    10801 cggagctaag ttagtagatg aaaatctaaa tgaacttttt gagtaacctg gttggtgaaa
    10861 atgaaaaaat ttgaaaaaaa gtgttgacaa gcatcccaaa agttgatata ctagtaaagt
    10921 aatcgacgcg gggatggcgg aattggcaga cgcgcaggac taaggatcct gtgaccgctt
    10981 taggtcgtga gggttcaagt ccctctctcc gcataggaaa agagctagct aaagctagct
    11041 cttttgtatt tctctaaaaa attagaatcc agtcaaatgt agtggagaat cagcgaaaat
    11101 aggctctttg tcaactgtag tgggttgaag aaaagctaag cttgagaaag gacaaatttc
    11161 gtcctttctt ttttgatgtt cagagcgata aaaatccgtt ttttgaagtt ttcaaagttt
    11221 cgaaaaccaa
//
LOCUS       DBDESG010000066        11205 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.066, whole genome shotgun sequence.
ACCESSION   DBDESG010000066 DBDESG010000000
VERSION     DBDESG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.066"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..731)
                     /locus_tag="ACKB38_001889"
     CDS             complement(<1..731)
                     /locus_tag="ACKB38_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980601.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQRLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVR"
     gene            1109..1447
                     /locus_tag="ACKB38_001890"
     CDS             1109..1447
                     /locus_tag="ACKB38_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGL7980602.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1486..1920
                     /locus_tag="ACKB38_001891"
     CDS             1486..1920
                     /locus_tag="ACKB38_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980603.1"
                     /translation="MGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINYIKV
                     GGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDLMVY
                     IFLGYVLVIQKDVYLFSILIIFSNFSVPFIREKEYELFKNKK"
     gene            2074..2286
                     /locus_tag="ACKB38_001892"
     CDS             2074..2286
                     /locus_tag="ACKB38_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980604.1"
                     /translation="MLIVLISVIVALLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            2660..2785
                     /locus_tag="ACKB38_001893"
     CDS             2660..2785
                     /locus_tag="ACKB38_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980605.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYLWA"
     gene            2766..3005
                     /locus_tag="ACKB38_001894"
     CDS             2766..3005
                     /locus_tag="ACKB38_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980606.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYPITSPIIGLVIGYNK
                     INLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            3151..4209
                     /locus_tag="ACKB38_001895"
     CDS             3151..4209
                     /locus_tag="ACKB38_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980607.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQ
                     SYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSKCMPSQYDR
                     FFYKDRPYIFSRKSSIKG"
     gene            4524..5501
                     /locus_tag="ACKB38_001896"
     CDS             4524..5501
                     /locus_tag="ACKB38_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980608.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFHFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISHYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            5878..6252
                     /locus_tag="ACKB38_001897"
     CDS             5878..6252
                     /locus_tag="ACKB38_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HGL7980609.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
     gene            6259..6441
                     /locus_tag="ACKB38_001898"
     CDS             6259..6441
                     /locus_tag="ACKB38_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980610.1"
                     /translation="MGIKMEKIFVIIFFVCLFISSITFLAYDFVSEEIKKLIIWINVV
                     FLILIIAMIIYPKLRK"
     gene            6460..7362
                     /locus_tag="ACKB38_001899"
     CDS             6460..7362
                     /locus_tag="ACKB38_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980611.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            7418..7771
                     /locus_tag="ACKB38_001900"
     CDS             7418..7771
                     /locus_tag="ACKB38_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGL7980612.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            7768..8496
                     /locus_tag="ACKB38_001901"
     CDS             7768..8496
                     /locus_tag="ACKB38_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980613.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSI
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            8975..9172
                     /locus_tag="ACKB38_001902"
     CDS             8975..9172
                     /locus_tag="ACKB38_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980614.1"
                     /translation="MKHFFAGIGEINFVSYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            9631..9870
                     /locus_tag="ACKB38_001903"
     CDS             9631..9870
                     /locus_tag="ACKB38_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980615.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            10423..>11205
                     /locus_tag="ACKB38_001904"
     CDS             10423..>11205
                     /locus_tag="ACKB38_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980616.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALV
                     VQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVP
                     EPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAK"
BASE COUNT         3776 a         1434 c         2073 g         3922 t
ORIGIN      
        1 cggacatagc agatagccag cctatcgtag cttttcttat tcttggcgtc ctccagtaaa
       61 gtgtagcaga tttgcttaca catgttttta tcttgattgt atagatagag tgtggaaagg
      121 tttagcagga ttgctattcg caagtcatat aaacgttgat agtttttata gacttctaag
      181 cgttgcaata tttttccagt gatgagatgg aggtattcaa tgggaaaact gaaaagaatg
      241 gtattgagga tttttagatc actctcatac catgtatctt gtttttcaat ttttctccaa
      301 agttttttga caacctgttc tgcgtgatct gatagttccc ctgccccgtg ttgacggaga
      361 taaatgacaa cttcaagcat atctctgatt tcttctatag ggagatcgtg gtgggtcttg
      421 aggtagtctt ggcatttttg aaataaattg acaagatcgc tggtcccgat aatggaacgc
      481 atattgagat aggtttgcat aatctctgtg cgttgacttg gttgataaag ttgacagata
      541 tattcaaact cttcaaaact catattgatt tgacggagaa gaaattccat attttcatat
      601 tttggggttg tcttgccact ttcgattttt gataagctgg ttcttgagag aacaccccca
      661 cagacctctt cttgggtcag cccctttgac tcacgtattt ctttatagac ctttccgaaa
      721 tcataacgca tgattttctc ctttctgtga aaaaagttaa cgtaatttta aaatgagctt
      781 aataagaggt atgatgtagg tagaaaataa agataggaga aaaagtatga ataaaaaaat
      841 attttgtatt ctagtatgta tgatatcatt gattagtctg gccataatct ttccatgggg
      901 ctggtcgata taatgtaagg gagcttgagt tgttaaagga ggcttagtcc ttggacaaaa
      961 atctgctgta ctacaatgct tggaaagata tcaagaacaa agaatgatac gattgaaaat
     1021 aaactgattc aagaactact gggcagtgat tgaggtttgg gtgacatcaa agaaaagtta
     1081 gattaattct aaagaattga gtatttctat gttaaaaaaa tatccttgta cgatgcaaca
     1141 tgatcagtca gattgtgctg cggcagttgt ttcaacagtt cttttatctt acaaaaagga
     1201 actatcaatc atgaagattc gggaaattat tggtacagac atgtatggaa cgactgtcag
     1261 tggtattgtt tcaggtctga ataagttgaa ttttacagta aaagctgttc gagtggcact
     1321 ggaagatttg actccaaaat taacatttcc tgcgattctt caagttaaga atgatttagg
     1381 tcaaaatcat tttgtggtat tacatagtat aaaggagaaa ataaatggaa ctcgtattac
     1441 caaataatta tgttgctctt gagcaagagg agatgatgta tcttgatggg gggggcttat
     1501 ttaagcaaga gtgcttgtca aggaatttgc gcagctttag ctatgagccc aggaactttt
     1561 atagcattga ctggagctgc agttttaaca aaaaaactaa taaactatat taaggttgga
     1621 ggccttggag gttggcttat tggtgcagca gcaggtgtat tggctcttat ctttttgata
     1681 aaaatttgtg tagatactac gataaaggtt ctattaaaca gaatctttaa ggcaagtaag
     1741 gtaatgaaaa ggaggatatt ttctcttttt gtactagatt tgatggtcta tatattttta
     1801 ggttatgttt tagtgataca aaaagacgta tatctgtttt caattctgat aattttttct
     1861 aatttttcag ttccctttat cagagaaaaa gagtatgaat tatttaaaaa taagaaatga
     1921 gatagatatg gtagctttat taaaactgag aataaagttt gggagatatt tttatttatt
     1981 ttaggtgtgt tgttaattgt aatttcatta ctaagtcccc caaaaattaa attttggaaa
     2041 tagagttagt gagaaggata ggataatagt attatgttga ttgttttaat ttcagttatt
     2101 gttgcattac tgtatcataa aattacagag tatgtcaaca acaggaaaat tgatattgtt
     2161 atttttctat cattgctgat ttctcatttt atttggaatt actatttttt accaattgaa
     2221 atattgatag caatcagtgg agcaaattta ctattaagtt ttttaaaaaa attacagatt
     2281 aaataatatt attccatata acatatcaac taggtcatta gattttattg aagatataaa
     2341 ttaaaaacga ggttgggaaa aaactcaatt tgaaaatgag attgagtaaa tctttctatc
     2401 tatataacgt atatcatcaa tatttttgaa cacctgataa tatgcatttt tcttatttta
     2461 aagatttttt gcctttcttc atttcttgaa tgaattggag agatgacttt atttaggtgt
     2521 ttgacattac tatagatagg atttagcagt actaatatga actcatatta taggtgcaga
     2581 cttaaatttt ggaagcaagc taaaggatat cttatctagg taactaagtg ctataacaag
     2641 atatattggt ggagttagta tggcaactat tactaatgct cttaatattg cggctactgt
     2701 tgctgaagtc ttctcattag gaggagccat tgcatatggt ttagatatcg tagacggtaa
     2761 atttgatggg tatttatggg cgtaaaacta tagctatgaa acattttttt gcaggaattg
     2821 gtgaaattca ttttttgtta tttttattat atccaatcac tagtccaatt attggactag
     2881 tgattggata taataaaata aatctaataa attctattat tatagctctt ggtatgatat
     2941 tggcattaat tttggtatta tcaagagtgt atagaaaata tttttataga gacgatgatg
     3001 actaaagttt ttatcaataa tttgggctcc ttgtcaactg tagtgggttg aagaaaagct
     3061 aacgtctggg gaaggggtta aagctttgct ttatcaaata ttgctttata gaaagtcctc
     3121 tgtgggattg aaaatgataa ggtgttttga atgaaagatg atcagaaata tttattagcg
     3181 ggactttatt cgcttctagt tgcaatattt tattttccat tgattgattc caaggggatt
     3241 tttgtaagta tattgatggc ggttttactt ttatatttaa tatacttcat tgcaacagtc
     3301 attcatattg tgataattaa gtttataaga aaaaaatctt ttaagtattt ggttctatat
     3361 ccatttactt atgatgggag ctggagattt caacctataa acttactcta ctttccagaa
     3421 atggtgagag atgtcattcc gattaattta gttcaagaat attgccaagg acaaccttac
     3481 ggactgttaa aaaagatgct gaaaagaatt cgattgtcac gagagatagc tttactcctg
     3541 gctaccatta tcgtctattt ttttacgcat agaattctac ctctttcagt atttacgttc
     3601 atgttctctt acatattgct atttgtacaa tcttatttgg gtagcaatac cgcttggatt
     3661 ggaaatagaa gattaataat agatgatgaa tttgaaaaaa tcttgctttc aaaaagttat
     3721 gtaaaagaaa tatcatctgc acgatactca gaatacttga catgtgaata caaaaacctt
     3781 acaccaatca tattgatagc tatttttgaa aatttgcttg actcctactt gcttcagaat
     3841 cagagtgagg ttgacttaga tattttttat aaagttttac ctttgcttta taaagaaaaa
     3901 tatactatgg gattcaatta ctttgtaagt ttaaattatt tactatataa ggttggattt
     3961 ttgggaatca tttacgataa tgaagcatta agagatttgt caaaacaata tttaaacaaa
     4021 aatatttcgg aattacaaga tggttctttc gagggcggta tacaagatgc tgttgcctct
     4081 aaacaaatag tagttataaa tgaatttata gcttgtttaa attccaaatg catgccatcc
     4141 caatatgata gattttttta taaagataga ccttatattt tttccagaaa gagttctatt
     4201 aaggggtgaa aatttgttac atttggatta acaagtgtcc tagggattag aaataactct
     4261 agtatagcta gatatttttg agtaactaga aatatcatta ggctactcat atgattgcca
     4321 gaaaagatgg ctgatgaatc ttatttatac tgttgcagta ggcgttgtct aggataaata
     4381 agctatcttg atgggggaga ttggaataat tttagtagaa acattaaagg attatgggca
     4441 agtagcacag gattaggtga tgcaatgtgc taagttggct tggtgttagc agtagtatta
     4501 tttaacaatg gaaagtagac taaatgaaaa ataaacgtta tttttttgac acaattttaa
     4561 tgattttact acttataagc acgatatttt gtgtcagccc agtatttata aaattggaca
     4621 tcttaggaac tccctctcat gccatcttaa cctttgtatt ggcgataccg ttattctata
     4681 ttttgtctca atgcttacat actttgctat tactagtttc ctcaatcttt tgtaaattaa
     4741 gaccaattta ttttcatttt atatttgtga tcattatagg agctaggaaa tactatcgaa
     4801 ttctttttca ccaactaatg ggatttagtc caggagtagc agtgttttat aaggagagtc
     4861 aaacaactaa gaatctgttt aaattttact acttcttgta ctttacaaca ctaatttcac
     4921 actatttttt ctttactttt gtgtatgaca aaccgttgct tttgcctctt attccatttt
     4981 ctattatcat agcattagta caaaaacttt atagaattga gaatcagcaa ttatttctgt
     5041 taaaatcaaa ggtactaaca atactggaat ctaagaagaa ttgtgagttt aatcttcagg
     5101 attatcatga aatttggaaa ttgcaatcaa aatctgagtt gccatgcgtg gctttatctt
     5161 atatcagttt gatcaagcca tatctttcag agagtgtgcg tgaacaaatt gatttattag
     5221 aagttaagcg atttaaaaaa ataaatcatc caatatcact ttatggtatg ttggatgtta
     5281 taaaattaaa tctatattta cggcattata atgaaaaaaa taaatatgaa tcaatgttaa
     5341 agaaaatttt agaggtaaga ccagactttg ttttaatcga gcagaatatt gatgatagtt
     5401 taaatagctc gcaaccatta tctttatctt tagccatttc agaaatccaa cttttgcttg
     5461 aagtgtatat ggggatcaaa catgtttcaa ttaggagata aaaccagaat aatatttgcc
     5521 attatagggg caagttgcat attagtcagt atgtatctta ttccacttat taaaactgag
     5581 aataaatttt gggaaatact tttatttata ttaggtgtat tgctaattgg aattgcgtta
     5641 ttaagtcaaa aaaaataaat tttgaaatag tattagtgag ttcgcaatca tttattgcta
     5701 gtggtttaga actgttaagt ttcaaacaat aattgatttt aaaccttagg taacctataa
     5761 aaacaaataa ggtagagaaa ggatatctta tctaggtagc taagtgataa aaacacagtc
     5821 gtctatcact tgttaggaaa ctatcctaac tatgcttaat aaaaaaggag aaaataaatg
     5881 gaactcgtat taccaaataa ttatgttgtt cttgagcaag aagagatgat gtatcttgat
     5941 gggggatttt ctattccgag atggcctgtt gcaacagcca ttaatatagc ttttaatggt
     6001 gttttaggtg gaggagcaat cagtctagtt agaaattata ttcgtaatta tggtttgcgg
     6061 cgagttacaa gcgcaattgc tggagcagct gcaagatatg ttggggtacg agttgcaaat
     6121 agagtggcag gatttgcact gtctgctatt aatggatttg cagcttggat gtcaattggc
     6181 aatgctatta caacaatctg ggccaataat gatgtaaata ggagagaccc aaatttaaac
     6241 gccttgtggt aactagatat gggtattaaa atggaaaaaa tttttgttat tatttttttt
     6301 gtatgtctat ttatatcatc cataactttt ttagcctatg attttgttag cgaagaaata
     6361 aaaaagttga ttatttggat taatgtcgtt tttttgattt taatcatagc aatgataatt
     6421 tatccaaaat taagaaaatg ataaggacac tgtaaaatta tgatacgtaa actaatcatt
     6481 tttttattaa tgctccctct ttttgtcttg tggctagagt tgcatttgtt agtgagtaac
     6541 ttgcaattga atctagaaat tccctttgat tttgtggtta gtacgagtct tacttttttt
     6601 gttttgatat tatcaaaaat agttttagac atactatacg cattgaaaga tttgtacaag
     6661 aaagaagctc ttattacgat ttttcctttt atttttatag gcaggaagaa agtaaatgtg
     6721 aggttctcgc cctatttttc attccatcgt aaaagtttgt cgcctgatga tttaagatct
     6781 aggattatat ggagttttat tcttgaaatt gctattatcc tagtatttat tttaaaaatt
     6841 ccttttgcaa tcatcatgct tactacaata tttttctgga ctataatgga tatcaatcat
     6901 ctcgttttta ataagacgga atttcttttt aatcaaaaca aatggcaaaa agaagattcg
     6961 tttgaaagtg atttgacgaa aacgttaaag gataagatac aaaaatcaga attaagctat
     7021 tctgacttaa tgtcccttct tctctatgat gcgatgaacc aatccacctt tttaacggat
     7081 agtgaacttt ttgaggatat tttgaaaaaa atcgaagatt ctcataacac ccttctgtgt
     7141 acaggtcttg tcgagttact gctgtatgaa atttctatta gtaagaatca taattggtca
     7201 gaaaagattg ataaaattag gatagagctt atcaagatta atcaattaga ttttttctat
     7261 tatacaagtt ggttaaggca aaattttgat ttctgtatga atagagagta tcataaaatg
     7321 aaatctcgga aattattact gagtaataaa aagatagttt aagttcgtcc tatttgtaaa
     7381 acaaatgaaa caatttcaac taaggaggag aaaacaaatg gaacttgtat taccaaataa
     7441 ttatgttgtg attgatgaag aagtaatgat gtatcttgat gggggagctt atttaagcaa
     7501 gcgtgcttgt caaggaattt gcgcagcttt agctatgagt tcaggaactt ttatagcatt
     7561 agctggagct gcagttttaa ccaaaaaact aataaactat attaagtttg gaggtcttgg
     7621 aggctggctt attggtgcag cagcaggtgt attggctaca gcagcaggaa aaatagctta
     7681 ctgtattgga tatggtgttc ttaatagagg ttgtgatatt aatgggaacc cttatccttg
     7741 ggatggattc atatctgcaa cagtaagatg agtaatgtag ataagattcg aaaaatacac
     7801 atcatcgtgt gttgggtgta tatattttta tcattcaggg cgattataaa tgatacggag
     7861 tattttttgc ttattttttt agcttttatc tactcaatag tatcactacc tctatattct
     7921 gtgaaaaata aaatagtatc tatctgtcta gccataaatt cgattctgtt aatcagtttc
     7981 cccattttaa tcaataagtt tttcccagaa agttttttga cttataccgt attaataagt
     8041 gtttttatta cagagttgat aatttttcat ttaattggga aagattttga tactaaattg
     8101 actaacgaat ataagaaaat tagtcagttt agaagcaagg tgtctcaatc tccttggata
     8161 aaatatttag agatttctag ttttatatta actatatttc catctattct ttatggtaca
     8221 gttgataatc atgtactgac tcttatcttt ttgataaaaa tttgtgtaga tactacgata
     8281 aaatttttat tcatcagatt atttgacaca agtactttaa tgaagaggag aatatttttt
     8341 cttttcgcat tggatgttat agcttactta tttttaggat atcttttagt gattcaaaaa
     8401 gcaggatatc tgttttcgat tttacttctt ttctctaatt tttcagttcc atttatcaaa
     8461 gaaaaggaat acgaattatt taaaaatagc aagtaataga aacataggtt aaatcttaga
     8521 acggcttaaa atcagatatg ataaagggaa atagtcttat ctaggtaact aagtgctaca
     8581 aacacaatcg aggatcactt gttaggatta cattttaatc atgcttaata agaatggaga
     8641 attcctatgg ggattgtatt actctgaagt ttcgaatacc ttagttctcg tgattttgag
     8701 ctattctagg tttagaatcc ttcagaatgt aaaattttaa gcagtcaaag acttagaata
     8761 gcctgaaaac atttatttta taacaattct aggcttagaa ttgctcatat atatgtattt
     8821 gagctgctcg atagttagga cagctctatt ctgagaaatc tgagctatca atgcttcaga
     8881 atagtatttc gccaatggtt tttattgaag aggtgattgt gttatgatag tctaaaataa
     8941 aattgattta atagagtaaa tggagaatcg ttacatgaaa catttttttg caggaattgg
     9001 tgaaataaac tttgtaagtt atttattgta tatatgtgta ggactagccc ctctttttca
     9061 tatatatgtc ataggttcgg aactgagttt tgtgaaaata gtgttatcta ttttgggagt
     9121 tatatttgta agtatgttaa ctattgctcg aatttatcgg acttttctat aaagaatagt
     9181 ttggacttga actgtatcta aaaacagagg ttggtagatt atcagcctct ttcttgtcgt
     9241 tgaaaagagt gagatatatt gacttttgaa aaaaatgtca taattgagtt gggataaacc
     9301 taaaagtaat aatatagtag attgaatctg gaatagtccg ttatgactgt taaaatattt
     9361 ctagaaatta atttgacttt cctaatcgat ttgttcacat cttgtttcaa tctactatac
     9421 tttctcgtta aaccgttcgg taattcatgg tgacatggac acatagtagt aagcacacta
     9481 tgtggcttgg cagatctaaa aactgttccc ttgcgataag cctaataagc acaacatagg
     9541 gagttagaga agccgactct aatcaaccac tttgggcagt agtggtaact gccccgtgct
     9601 ttttattttg agaaaatatg gagtttgtcg ttgaaattac ttgattgtat tttggattat
     9661 caagaaagat tcaatggaaa aacatgtcaa gtatcaacga attataagta tttagagatt
     9721 ttcaaagtga atttttgctt gactgattta catcatttat ttgacttaca caaaatcaca
     9781 cgagattatg ctagtcaaac aaaacctgct attcaagatg gtgtttttat tttagaagat
     9841 tttagaaata tcctatgtac aatgatgtaa tcgaaaggat atctttatgt gagtttatcg
     9901 gcgatatctt ctatatctaa gctagcaagt tgttgcatcg tagctaagga tttatctaaa
     9961 aatactatga aattggacgt catatttttt gaggataaaa ataaaagatc cgcagtttta
    10021 ggtttacgaa gagacaaaag cggagtattt aaaccagtta ctctacattt tacaagcgct
    10081 aagaaatatg ctaaagttcg taaaacagat gtgaaagaaa tgaaatggct ataaagattg
    10141 tccgcaggct taagcttgcg ttttttgttg tttaacgaaa ttaaaaaaca agctctctca
    10201 tcggaaatgt ttttatagga atgaaggaag atgatgctat gtttaaaaag agaatcgaaa
    10261 agggcaaaag tagtatcttt atctattttt tggagtagaa gcttatgata tagaaatttg
    10321 taataaaaat gtaatataaa acatttgaca aatatttacg gggggagtat acttaatata
    10381 attatagtct aaaaatgact atcagaaaag aggtaaattt agatgaataa gaaaaaaatg
    10441 attttaacaa gtctagccag cgtcgctatc ttaggggctg gttttgttac gtctcagcct
    10501 acttttgtaa gagcagaaga atctccacaa gttgtcgaaa aatcttcatt agagaagaaa
    10561 tatgaggaag caaaagcaaa agctgatact gccaagaaag attacgaaac ggctaaaaag
    10621 aaagcagaag acgctcagaa aaagtatgaa gatgatcaga agagaactga ggagaaagct
    10681 cgaaaagaag cagaagcatc tcaaaaattg aatgatgtgg cgcttgttgt tcaaaatgca
    10741 tataaagagt accgagaagt tcaaaatcaa cgtagtaaat ataaatctga cgctgaatat
    10801 cagaaaaaat taacagaggt cgactctaaa atagagaagg ctaggaaaga gcaacaggac
    10861 ttgcaaaata aatttaatga agtaagagca gttgtagttc ctgaaccaaa tgcgttggct
    10921 gagactaaga aaaaagcaga agaagctaaa gcagaagaaa aagtagctaa gagaaaatat
    10981 gattatgcaa ctctaaaggt agcactagcg aagaaagaag tagaggctaa ggaacttgaa
    11041 attgaaaaac ttcaatatga aatttctact ttggaacaag aagttgctac tgctcaacat
    11101 caagtagata atttgaaaaa acttcttgct ggtgcggatc ctgatgatgg cacagaagtt
    11161 atagaagcta aattaaaaaa aggagaagct gagctaaacg ctaaa
//
LOCUS       DBDESG010000067        11105 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.067, whole genome shotgun sequence.
ACCESSION   DBDESG010000067 DBDESG010000000
VERSION     DBDESG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.067"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..102
                     /locus_tag="ACKB38_001905"
     CDS             <1..102
                     /locus_tag="ACKB38_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGL7980617.1"
                     /translation="ILGHKDISMLIDVYGHTLEEKISEEFMAVKSLL"
     gene            complement(185..257)
                     /locus_tag="ACKB38_001906"
     tRNA            complement(185..257)
                     /locus_tag="ACKB38_001906"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Lys,seq:ctt)
     gene            complement(522..1793)
                     /locus_tag="ACKB38_001907"
     CDS             complement(522..1793)
                     /locus_tag="ACKB38_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGL7980618.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            2085..2306
                     /locus_tag="ACKB38_001908"
     CDS             2085..2306
                     /locus_tag="ACKB38_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980619.1"
                     /translation="MEMMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEI
                     LDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            2471..2650
                     /locus_tag="ACKB38_001909"
     CDS             2471..2650
                     /locus_tag="ACKB38_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980620.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            2787..2966
                     /locus_tag="ACKB38_001910"
     CDS             2787..2966
                     /locus_tag="ACKB38_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGL7980621.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            3003..3455
                     /locus_tag="ACKB38_001911"
     CDS             3003..3455
                     /locus_tag="ACKB38_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGL7980622.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            3746..4216
                     /locus_tag="ACKB38_001912"
     CDS             3746..4216
                     /locus_tag="ACKB38_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGL7980623.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKIGLEKGEFYPYPRY"
     gene            4235..5323
                     /locus_tag="ACKB38_001913"
     CDS             4235..5323
                     /locus_tag="ACKB38_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGL7980624.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENWLAELEKEMLEGTEDGTRNF"
     gene            5304..5732
                     /locus_tag="ACKB38_001914"
     CDS             5304..5732
                     /locus_tag="ACKB38_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGL7980625.1"
                     /translation="MELEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            5868..6818
                     /gene="prmA"
                     /locus_tag="ACKB38_001915"
     CDS             5868..6818
                     /gene="prmA"
                     /locus_tag="ACKB38_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGL7980626.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKL
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            6820..7563
                     /locus_tag="ACKB38_001916"
     CDS             6820..7563
                     /locus_tag="ACKB38_001916"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGL7980627.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            7574..9370
                     /gene="pepF"
                     /locus_tag="ACKB38_001917"
     CDS             7574..9370
                     /gene="pepF"
                     /locus_tag="ACKB38_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGL7980628.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            9616..10170
                     /locus_tag="ACKB38_001918"
     CDS             9616..10170
                     /locus_tag="ACKB38_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGL7980629.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            10281..10949
                     /locus_tag="ACKB38_001919"
     CDS             10281..10949
                     /locus_tag="ACKB38_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGL7980630.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         3211 a         2015 c         2640 g         3239 t
ORIGIN      
        1 attttaggac ataaagatat ctccatgttg attgacgttt atgggcatac cttggaggaa
       61 aaaattagtg aggagtttat ggctgtgaaa agtttgttgt aaaaacacgg caaattgcac
      121 gacaattttt aaaaggaaag caaaaaaagc cctatattat aaggcttttc ctgttgtatt
      181 tatatgcccc ctacagggat cgaacctgtg acccacggat taagagtccg ctgctctgcc
      241 agctgagcta aggaggcaaa gaaaaaagct gtattggtgc cgaaacttca cggtttgtat
      301 tgaacccgcg caattaagca ggtgggcaac tcgctctaac tgaagctgtt tccgtgtgag
      361 acggcctaca tgctgttaga agacttttgt ttccctaata atacaaaaaa tagtcggtca
      421 acacttaagt gtgaagtcgt acaccacagc gtttctatat ttatatgata ccactttttc
      481 aaaaaaaatc aagaggaaag tgcaattttt tgaaaaggat ttcagatttt tcgcaaacgg
      541 tcgatagctt cctttctttg agccaaagcc cgttcatatt taccagtatc ttctgcttgg
      601 aaatagtgat gattacgaat tttttctggc agatagtctt gcttgaccca atttccagga
      661 tagttgtgtg gatagagata gtcttgggca ttccccagtt ccttgcttcc actgtagtgc
      721 ccatcacgca ggtgtcgcgg aataggcaag tgccctgatg ttttgaggtc agcaagtgcc
      781 ttatccatag ctacataggc tgagttggat tttggagaaa gggccaaatc aatcacgaca
      841 ttggcgatga gaatgcgggc ttctgggaac ccaatcttct gggcagcatc cagagcagtc
      901 acggtatgaa tctgggcctc aggattggct aagccgatat cttcataggc gataacagtc
      961 aagcgacgag cgagactagg cagatcccca gcctcaatca agcgggcagt atagtggaga
     1021 ctggcatcca catctgagcc acgaatagac ttttgcaggg cagatagaac atcatagtga
     1081 ccgtctccat ccttgtccat agtgatgtag cttctctgaa gactattttc catgatgtct
     1141 aaggtgatgt ggcgaatgcc ctcgtcattc tcaggggtag agagaacagc taagtccagt
     1201 gagttaaagg cagagcgaag gtctccgttt gtagaggttg cgatgaaatc cagcgcatcc
     1261 tcatctagtt ctattggaaa atcaaaacca cgttcagggt tacttagagc tatctgcagg
     1321 gcctctttga cgtcttggtt agacagaggt tccaactcga aaatttgaac tcggctacga
     1381 atggcaggag tgacagagaa gaaaggattt tcagtcgttg ctccaatcat gatgaccagt
     1441 ccactttcca agagaggcaa gaggaagtct tgcttggtct tatctagtcg atgaatttcg
     1501 tctagtaata ggacgagacc accagaaaat ttagcttctt ccgagatttc ttgcagtcgc
     1561 tttttactat caactgtcgc attgaaagtt cgaaaggcat acttggtcgt tccagcgatg
     1621 gcagaggcaa tactggtttt gccgattcca ggagggccat atagaatcat ggaggacaag
     1681 cggttggctt ccaccatgcg gcggatgatt tttccaggtc cgaccagatg ctcctgaccg
     1741 atgacctggt cgatggtttt agggcgcatg cgaagcgcga gattgtctgg catagcagtc
     1801 ctttctaacg tggatttgag tagaatgact catctactat atcttactat gtccattcta
     1861 gcctattagt tgtgatattt caatggtttt gtaataatca gggagtgaga tagaatcttt
     1921 tttactattt aatttaatga ggagaggtat cagcatgaga gatttgattt tccttaaagc
     1981 tcaaaatagg atagttgagc ctaaagaagt ggaagtgctg acttgagaaa taatattgct
     2041 gaatgtcatt gtatgaacta taatgaaaag agaggtgata agcaatggaa atgatggaac
     2101 taccaagtca ggaaattttg atttttacaa aacaaattcg tcattggatt cttagtgatc
     2161 aagttatttc agggaaaaga aaacttttct tccgggaaga tactccaaaa gagattttag
     2221 atttgtacga aaacattaaa tctaaacttg attttgctta ccaagaagtt cattctaata
     2281 atgggttgaa aaaatatgaa aaatagaata attgatgttt ttgaagtagt aaatcgcctc
     2341 cttgtaataa ctgttgaaaa tcctgatttc gaagacttga gagttaatca tactcaatga
     2401 aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg taaggcgacg
     2461 ttgacgtggt ttgaatttga ttttcgaaga gtatcagttt gtaaaaattg gagataaaaa
     2521 atatagagta cgcagtgttc ctatgattca ttcaactccg cctcaatctg tcttagatcg
     2581 agatactttt acaattgact atacagatga tgaattgctc gataaagaag cagtttttac
     2641 tactcattag aaaaataggt atgaataaat tggcttccct tagaaccaca tctaggggct
     2701 tttatgtata atagaataat aagtatcact cctttacaat acgtgtaaca cgtgttataa
     2761 tggtgttgtc aggaggtaaa agcgctatgc ctatgacaca aaaagagatg gttaaactcc
     2821 ttacggccca tggttggata aagactagag gcggtaaagg ctcccatatt aaaatggaaa
     2881 agcaagggga aagacctatc acaatccttc atggtgagct gaataagtac actgaaagag
     2941 ggataagaaa gcaagctggg ttgtaaaatt cagtccctgc tttttgatat ggaggtagtg
     3001 aaatgttagt tacgtatcca gctttatttt attatgacga cacagacgga acagaagcga
     3061 cttattttgt ccatttccca gattttgaat actcagctac acaaggagag gggatttctg
     3121 aggctttggc tatggggtcg gagtggctag ggataactgt tgcagatttg atagagagtg
     3181 atggagagtt acctcagcca tcagatatca atagcttgtc tttaattgat aatgatcctt
     3241 ttaaagatga tgaagatttc gtgtcaacct atgaccttga taaatctttt atttctatgg
     3301 tatctgttga tgtatcagaa tacctaggaa gtcaggaacc cattaaaaag actttgacca
     3361 taccaaaatg ggcagataag ttgggacgag aaatggggct taacttttct cagactttga
     3421 cagacgctat tgcagataag aaagttcaag cctaagatta agcgtggtat cgtggtaata
     3481 gtcaataaaa aagcacgtcc acttgtgcta gttactcgcc tattgatttt atagatttat
     3541 tagccctttc gagggctttt tatattgatt tttatgagaa atgaagaaat cagactttta
     3601 agaaaatcac tgataccaag ggtttaaatg aggtaatatg gtataattag gacataaaat
     3661 aattttgtgg taagatggta gtatctattt tagcatattt ccgagcaatc ggggcgatta
     3721 aagagtcgca tagaaagagg acaaaatggc aacatacgga tttttagata ttttagagga
     3781 agagttggac aagaactttc cctttgactt tgagattagc tgggacaaac gcaaccatgc
     3841 ggtagaagtg agttttctat tggaagcgca aaatgctgca ggtgtggaga tggtggatga
     3901 agacggagag gtttcatcag atgacattct ttttgaggaa gcggtccttt tctacaatcc
     3961 tgccaagtca acagtcaatg aggaagacta tttgactgtt attccttacc tgcctaaaaa
     4021 aggattttcc cgtgaatttt tagcttattt tgcgcttttc cttaaagata ctgctgaggt
     4081 tgggctagat gtcctcatgg actttttgga agacccagaa gcagaggaat ttgtcatgga
     4141 atggaaccaa gaagtctttg aagaaggaaa aattggcttg gaaaagggag aattttatcc
     4201 ttatccaaga tactaggagt tggttggagg tgttatgaag aaaattggga cttatttggt
     4261 ttatgtgcta gctgttgtct ttattatgct agcttttgct tgtggaacaa tcgcatttgc
     4321 agagttgggg tattccgcag ttttagcctt tacttttggt tatgccttcg ctcttctaag
     4381 catgtattta atctttattc ttcatgagct gggtcatgct ttttgtggct acttgacagg
     4441 ctatcggctg gtggcttttg gattaggaca ttttattttg accaaaaagt caggcaggtt
     4501 tcatcttagt agaacagcca ttctgaaaaa tgttggtgct caatacattg gtttaaaaga
     4561 ggatgaaagc gatcaaagaa tcatcctgat gctttcagga ggcttgatgg ttcatctcgg
     4621 cttgatatta ttggcgatat tgtttggatt tttgacaaga agctggtatt ttgcagggac
     4681 ttggattttt cttaatttat ctttcttcct aaataacatt ttgccagtcg acatcaccga
     4741 tggagcaaaa atttgggaat tactacaaca ccctgaaaat accaaatacg cctacctggt
     4801 attgagacat tctgcccaga ccttgctagc tcctcaagaa tatgatttga aagactttgt
     4861 tcaagcagtt cctgaggatg cacgagggag ttttgcggat ggtgtattag gtatgcaagg
     4921 tgaagtttcc attttggaag ggaaggaaga agtagctaaa cagcagtttc aagcattact
     4981 tgagaggaca actactccga tgatgcagac agttgcccaa ctatccctgc ttcatatcgc
     5041 cttgttggaa ggagattttg agcaagcgga gcaatatgct agtattcgac aggtcaaatc
     5101 gtttctgtct ctcaaactgt ccaatcttca agtgatacaa gcctggtatc aattaaaggt
     5161 gaagaaagat gtagatcgaa ctcgtaaggc tctcaagatt gctagacaga aaatggatag
     5221 tagtcgaatg ttgagggatg agaaaagata ttatgaaaac tggttagccg agctggaaaa
     5281 ggaaatgtta gaaggaactg aagatggaac tagaaatttc tgatttcaca ggttgcaaga
     5341 ttgctttgtt ttgtggggat aagcttttga ctatcttacg cgatgataag gcaagtattc
     5401 cctgggccaa tatgtgggaa ctgccaggtg gtggccgtga aggggacgaa agcccttttg
     5461 aatgcgctcg gcgtgaagtt tatgaggaac tgggaattca tttagatgaa gactgtctgc
     5521 tttggagcaa gatttatcct agcgtaattt ttaaaggcaa gaaatcggtc tttatggttg
     5581 gtcagctaag acaagaacaa tttgacaata tcatcttcgg agatgaagga cagggctatc
     5641 agctgatgaa tgttgaggaa tttcttagtt ccagtcaagt tgtgcctcag ttgcaagaga
     5701 gattaaaaga ttatttaaaa gtaagtgatt agaaaggatg gttcagttac atttctaaac
     5761 tgaacccgcc ctaaacactg tgccaaaaag ataaacttct cttagacaca agcgtcttca
     5821 gagaatttcc tattttggtt ttgtgtttta cgggcttggt attaataatg gaaacatggc
     5881 aagagttaaa agttacagtg aagcgtgagg gagaggagtt ggtttccaat ctcttgattg
     5941 agctgggagc gcaaggagtc gcaatcgaag acagtatgga ctatgtggga aatgtggacc
     6001 gctttggaga gattttccca gaggtggagc agcaagaaga aatcgtagtg acagcctact
     6061 accctgacac ggtggatgtg acagtggttg aggcggattt gcaggctcgt ctagcagaat
     6121 tgacagattt tatggatttg ggagagttaa aaataggtac gactgccttg gctgaggaag
     6181 actgggcaga caactggaag aaatactatg aacctgctcg tatcactcat gatttgacca
     6241 tcgttccctc ttggacagac tatgaggcga ctgcggggga aatgattatc aagctggatc
     6301 ctggcatggc ttttggtact ggaacccacc caactaccaa gatgagcctt tttgccttgg
     6361 aacaggttct tcgtggtggc gaaacggtgc tagatgtggg gactggttca ggggttctct
     6421 ctattgccag ctcgcttctg ggggctaaag aaattttcgc ctatgacctg gatgatgtgg
     6481 cggttcgtgt ggctcaggaa aatattgagc tcaaccctgg catggaaaat atccatgtag
     6541 cggctggaga tttgcttaag ggtgtggaaa ttgaggcaga tgtgattgtg gctaatatct
     6601 tggcggatat cctcattcat ctaatagacg atgcttatcg cttggtaaag gatgaaggct
     6661 acctgatcat gagtggcatt atcaaggaca agttggacat ggtgcgccag tcggctgagt
     6721 cagctggatt tttcctcgaa actcacatgg ttcaagggga atggaatacc tgtgtcttta
     6781 agaaaaccaa ggatatctct ggtgtgattg gaggctagca tgcagcagta ttttgtaaaa
     6841 ggcagtgcta tctctcctgt cactatcgag gacaaggaaa ccagtaagca tatgtttcag
     6901 gttatgcgct tgaaagaaga ggatgaggtt accttagtct ttgatgatgg catcaagcgc
     6961 ttggcgcgcg tgctggatat ggaaaatcgt cagtttgagt tggtccaaga attaaatgac
     7021 aatgtggaac taccagtcca agtgaccatc gcatctggat ttcccaaggg agacaagctg
     7081 gagttcatcg ctcaaaaagt aaccgaactg ggggctagcc aaatctgggc ctttcctgca
     7141 gactggtcag ttgccaagtg ggatggcaag aaattgggta aaaaagttga aaaactagaa
     7201 aaaattgccc ttggagcagc cgagcaaagc aagcgtaata ttgtaccaag tattaaactt
     7261 ttcgagaaaa aagcagattt tctagctcag ctggaccagt ttgactctat catagtagcc
     7321 tatgaagaat cagctaaaga aggagaagcc gctgcgcttc tgcaagcagt cactggtctt
     7381 gaaacaggag ccaaactgct ctttatcttt ggtccagaag gcggtctctc acctgcagaa
     7441 atcgaaagtt ttgaagctaa aggagccgtt ttggcaggcc taggtcctcg tattttgcga
     7501 gcagaaacag caccgcttta cgctttatca gcccttagtg ttttagtaga attagagaaa
     7561 taagaggaag aaaatggaac aaaaacaccg ttcagaattt ccagagaagg aactctggga
     7621 cttaacagcc ctataccaag accgtgagga tttcttgcgt gcaatcgaga aagctcgcga
     7681 agacatcaac cagtttagcc gtgattacaa gggcaatctt cacacttttg aggatttcga
     7741 gaaggccttt gcggaattgg aacagatcta cattcagatg agccatattg gcaactatgg
     7801 ttttatgcct cagacgacgg actatagcaa tgacgaattt gccaatattg cccaagctgg
     7861 gatggaattt gaaacagatg ccagcgtagc cttgaccttc tttgacgatg ccttggtggc
     7921 agcagatgag gaagtcttgg accgtttggg taaattgcca catttaacag ctgccattcg
     7981 tcaggctaaa atcaaaaaag cccactactt aggggcagat gtggagaagg ccttgacaaa
     8041 tctcggtgaa gttttctaca gtccgcagga catttatact aagatgcgag ctggggattt
     8101 tgaaatggct gactttgaag cccatggcaa gacctacaaa aacagctttg tgacctatga
     8161 gaatttctac caaaaccatg aggatgctga ggttcgtgag aaatccttcc gttccttctc
     8221 agagggactt cgtaagcacc aaaatacggc tgcagcagcc tatctggctc aggtcaagtc
     8281 tgaaaaactc ttggctgata tgaagggata cgactctgtc tttgactatc ttctagctga
     8341 acaagaagtg gaccgtgtca tgtttgaccg ccagattgac ctcatcatga aggactttgc
     8401 accagtcgct cagagatacc tcaagcatgt tgccaaggta aatggtcttg aaaagatgac
     8461 ctttgcagac tggaaattgg acttggacag cgccctgaat cctgaagtga ctattgacga
     8521 tgcctatgat ttggtcatga agtcggtaga acctttgggg caagaatatt gtcaggaagt
     8581 tgctcgttac caagaagagc gctgggtgga ctttgctgct aacagtggca aggattccgg
     8641 tggttatgcg gcggacccat atcgcgtaca cccttatgta ctcatgagct ggacaggccg
     8701 tttgagcgat gtctatacct tgattcatga aatcgggcat tctggtcaat tcatcttttc
     8761 agacaatcat caaagttact tcaatgccca tatgtcgacc tactatgttg aagcaccgtc
     8821 aaccttcaat gaattgctac tcagtgatta cttggagaac cagtctaatg acccacgtca
     8881 aaaacgcttc gctctggctc atcgcttgac agacacctac ttccataact ttatcaccca
     8941 cctcttggaa gccgccttcc agcgtaaggt gtatacattg attgaagaag gggagacctt
     9001 tggagcaagc aagctcaaca gcattatgaa ggaagttttg acggatttct ggggagatgc
     9061 tattgaaatt gacgatgatg caactctgac ttggatgcgc caagctcact actatatggg
     9121 cttgtatagt tacacttact cagcaggact agttatctcg actgctggtt accttcatct
     9181 aaaacattct gaaactggag ctgaagactg gctcaatctc ctcaaatcag gtggtagcaa
     9241 gacaccactt gagtcagcca tgattatcgg tgctgatatt tcaacagaca aaccactccg
     9301 tgataccatc caattcttgt ctgacacagt tgaccagatt atctcctata gtgctgagtt
     9361 gggagagtag gggaatagaa aaatttttta gaatgatgtt tatgggcttg ttaagcgtca
     9421 ttctatttgt cttagttgta gctattttca aaaatgtcgt tgaaggtcgt gatgttaaat
     9481 ttgaacttac tgtagaactg gtattgatag ttatttacgt cattttatat cagattgtat
     9541 ttaattgaga attcaatatt gagtcattta agactgcctt tttgaacagt tgctagaaat
     9601 gtagaggtat tgaccatgta tcaagattta ctaagaaaaa tagcagaaga aaaaccaaat
     9661 tataaccagg aagaaatcca gtggttgttt gatcatttgg gaaatccttc tccagaaatt
     9721 cgtaatgtcc ttttaaatca aggcttgcac tatctttcaa aagaaaagga tacaaggggt
     9781 ttctcaagtc agtatggttg ggttcacgct tttgcacatg gagccgattt actgacagag
     9841 gtggtttgtc atccagactt tcctaaaaac agagttcatg aagtatttga tatacttggc
     9901 caactattta aaagaatgtc gattcgcttt acagatgatg aggattggcg tttagcaaga
     9961 gtgatctatg aacctatttt acaagggaag ttggagcaag aacaagtagc ttcttggata
    10021 aaaactgttg attttccgat agaagaaagg gaggattttt acaaattttc caactttaga
    10081 tcctgtctgg tggaagtcta tgtccaactt gaccagagaa atagtttaca agatgacctg
    10141 aaagaagcta tccagtcttt tcaatactag ggattagcta atagttctat gattttcaaa
    10201 aaatttccaa tcaattttct tgaaaccctt tcctactttt gatagactag tatctagttt
    10261 ttgaaaaaag gagaaagaaa atgaaaaaat ttgttgctga gttaatcggt acgttcatgc
    10321 ttgtattcgt cgggacagga gctgttgttt ttggaaatgg tcttgatggc cttggtcacc
    10381 ttggaatcgc ctttgccttt ggtttggcaa tcgtggtggc agcctactca atcggaactg
    10441 tttcaggtgc tcacttgaac ccggctgttt cgattgctat gtttgtaaac aaacgtttgt
    10501 catcttcaga acttgtaaac tacatccttg gtcaggttgt tggagctttc atcgcttctg
    10561 gcgctgtctt cttcctcttg gctaactcag gtatgtcaac tgctagtctt ggtgaaaatg
    10621 ccttggcaaa cggtgtcact gtctttggtg gtttcttgtt tgaagtcatc gcaactttct
    10681 tgtttgtatt ggttatcatg actgtgactt cagaaagcaa gggcaatggc gcgattgctg
    10741 gtttggtaat cggtttgtca ttgatggcga tgattcttgt cggattgaag attactggac
    10801 tttcagtaaa cccagctcgt agcttggcac cagctgtctt ggtaggcggc gcagcccttc
    10861 aacaagtttg gattttcatc cttgcaccaa tcgctggtgg agttcttgca gcccttgttg
    10921 caaaaaattt ccttggaaca gaagaataat tgaaactcaa aaagccttgc tcctcatctt
    10981 gaggaacagg gctttttcgt atgatactct tcgaaaatct cttcaaacca cgtcagcttc
    11041 atcttgccgt aggtatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt
    11101 tttga
//
LOCUS       DBDESG010000068        10645 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.068, whole genome shotgun sequence.
ACCESSION   DBDESG010000068 DBDESG010000000
VERSION     DBDESG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.068"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(65..589)
                     /locus_tag="ACKB38_001920"
     CDS             complement(65..589)
                     /locus_tag="ACKB38_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGL7980631.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(642..878)
                     /gene="rpsT"
                     /locus_tag="ACKB38_001921"
     CDS             complement(642..878)
                     /gene="rpsT"
                     /locus_tag="ACKB38_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGL7980632.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(946..1866)
                     /gene="coaA"
                     /locus_tag="ACKB38_001922"
     CDS             complement(946..1866)
                     /gene="coaA"
                     /locus_tag="ACKB38_001922"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGL7980633.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2056..2459
                     /locus_tag="ACKB38_001923"
                     /pseudo
     CDS             2056..2459
                     /locus_tag="ACKB38_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2666..3256
                     /locus_tag="ACKB38_001924"
     CDS             2666..3256
                     /locus_tag="ACKB38_001924"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGL7980634.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3253..4530
                     /locus_tag="ACKB38_001925"
     CDS             3253..4530
                     /locus_tag="ACKB38_001925"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGL7980635.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4548..5210
                     /gene="deoC"
                     /locus_tag="ACKB38_001926"
     CDS             4548..5210
                     /gene="deoC"
                     /locus_tag="ACKB38_001926"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGL7980636.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5197..5586
                     /locus_tag="ACKB38_001927"
     CDS             5197..5586
                     /locus_tag="ACKB38_001927"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGL7980637.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5676..6728
                     /locus_tag="ACKB38_001928"
     CDS             5676..6728
                     /locus_tag="ACKB38_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGL7980638.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAERGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6872..8407
                     /locus_tag="ACKB38_001929"
     CDS             6872..8407
                     /locus_tag="ACKB38_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980639.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8400..9458
                     /locus_tag="ACKB38_001930"
     CDS             8400..9458
                     /locus_tag="ACKB38_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980640.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9461..10417
                     /locus_tag="ACKB38_001931"
     CDS             9461..10417
                     /locus_tag="ACKB38_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980641.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            10502..>10645
                     /locus_tag="ACKB38_001932"
     CDS             10502..>10645
                     /locus_tag="ACKB38_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGL7980642.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDR
                     YGIE"
BASE COUNT         3050 a         1891 c         2423 g         3281 t
ORIGIN      
        1 gctccataat atctatagtg gatttaccca ctacaaatat tatagagccc aaaaaggaag
       61 ccctttatga attgtaggac ttccttttct tatccagaaa ttgatctagc tctctctgat
      121 ttcgaagaat agtgacttta tgtgaatatt cttggcaaag tttttggtaa ttttcttttt
      181 gagttttgct acgcccatcc caaagaatcc atctgataaa ctcccactca aagcgttcag
      241 ggcaatctac cgccatactt tctctgactt ttccacggta tttaagataa cgcttaaagg
      301 ctctaaagag acaggtcaat ggcgaaaaat tgagaaagat gatttggtca gcttcttgca
      361 ttcgttcttg gtagtagcac caagaataat taccatcgat gacccaagct ttatgcttgg
      421 tgagaaagtt ttttatctcg gttaacatcc attcgcagtc actgtcttgc caaccaggtt
      481 gaaattggag tgtgtccatg tgcagttttg gaatggagta gtagttagat aacttttctg
      541 ctatagttga cttaccagaa ccagaatatc cgataattgc gattttcatt ttctaccttt
      601 tcctatttgg agacaaaaaa acagcctcta tggactgttt cttatttaac aagtttagct
      661 gaaagacgag ctttatcgcg gcttgctttg tttttgtgaa tcaaaccttt agtttctgct
      721 ttatcgatag ctgagctagc agcacggaaa agttcttcag atgggtttgc ttcgaaagct
      781 tttatagcag tacgcatagc tgatttttga gctgagttct tttcgttttg tttaacgttc
      841 aattcagcgc gtttgatagc tgatttaatg tttgccaatg ttcttacctc catatttact
      901 aactatacca ttatatatga aaacttacgt tttgacaagg gaaaattact tttttaagta
      961 aatttcatcg atttcatggt tcttgctttt atgaagaatc acttctgcac gatttctggt
     1021 tggttcaata taattttgca gatttgtgag attgatactg atccagacct gatgggcaaa
     1081 ggactccact tccccaatcg gcatctgagt aaaacgataa tagtagctat cagggtcgtt
     1141 ttgggctaga ctcagcattt tcaagaaacg gtccagatac caactttcaa tatcatccac
     1201 tccagcatct acatagatgg aaaagtcaaa gaagtcagtg atatagagac gatcgttttg
     1261 tggattttga aagacattga ttccctcaac aattacaaaa tcagcagctt tgacactttg
     1321 tttctcttcg ggtacgatgt cgtaaacttc atgagaatag acaggaatat ctacatcttg
     1381 tccatttttg atgcggtcca agaagttgag aagagcttcc atatcatagc tttcaggaaa
     1441 tcctttacga tttaaaatcc cctgctcaat caaggtttga ttgggataga gaaaaccatc
     1501 agttgtaacc aactcaaccg tagcatctgt aaacgtacgg gacagtagga tttgaagtag
     1561 gcgactggtt gtggattttc caacggcaac actcccagaa accccaataa taaaaggttg
     1621 agatttactt tcacgttgga ggaaaattcc ttttgaaaag gctaaatctt ccttagttcg
     1681 cttgtaaatc tgaatcaaat gagccaaggg gagatagata tctgtaacgt cttggagact
     1741 gatttggtca ttaaaactct tgatagattc caattcttct tctgtcaaag gaggtgttgt
     1801 ctttcgatgt aaagattgcc aagtctggcg gctgattttt tcaaaatgta aaaattcgtt
     1861 ggtcatttgt tttcccctag atattttgtc tatcatacca taaaaagcta aaaaaataaa
     1921 gcggtttctt gaatgtggta agtcaatggt atatagtata agtgatcaat ttattgatga
     1981 aataggagat agattctact gatttataag cttataaagg gggcttttta gttcacaact
     2041 tataggaggt gtactatgaa aatcttaaaa cgttacatat tggaactctg ttttatttta
     2101 agttttgctt taccttttat aaaaggaacc aatgcagata atggtagatg ctttgtggaa
     2161 acctattacg gttttacttt tttgatggaa catgctattg taacagctgt ctttatttgt
     2221 tcgttcttaa ttgctttctt actaaaaaac gatggacgaa atggattgct gcgggtagtt
     2281 attgcttttt agtcctacgg attgcgacag agggttattt cttccacatg tcactagaag
     2341 atttgatacg actttggaca agtttggaaa tcctgacaca aacgtatcag ttgggctttt
     2401 atctaaacat tttgttagga attttgttga taataaagta ttttaaggtt aagcaataga
     2461 aataataatc tgcctgatta tagatgataa actttttgtt tttatctgga ctgtgatatt
     2521 tatagttcta aatggtttat tatttataag tgttgaaaga gattgaagaa aatggcttcg
     2581 tgacttgctt tcttcggtct ttttcgtttt attatcaatt tgtaaacgat ttacaatttg
     2641 agtcaaatta tggtaaaata gtgtcatgag taaaatgtac tatgcagaaa atcctgacgc
     2701 tgctcacgac attcatgagt tgagagtgga cttgttggga gaaaaaatga cttttttgac
     2761 agatgcgggt gtttttagca agaaaatggt tgactttgga agtcaactct tgctcaagtg
     2821 tctggatgtc aaccaaggag agacagtcct agatgtaggc tgtggttatg gtccattagg
     2881 tttgtccttg gtcaaggttt acggagttca ggcgaccatg gttgatatta acactcgtgc
     2941 cctggattta gctcagagaa atgctgaaaa aaataatgca aaagcgacga tatttcaatc
     3001 taatatttat gcaagagttc aagggcgatt tgaccatgtc atttccaatc ctcctatccg
     3061 tgcgggcaag caagtcgttc atgaaatcat cgagaaaagt aaagatttct tggaaattgg
     3121 tggagatttg accatcgtca tccagaaaaa acaaggagct ccaagtgcca agtctaagat
     3181 ggaagatgtg tttggcaatt gtgaaatcct taaaaaagac aagggatact atatccttag
     3241 aagtgtgaaa gaatgagagc agttgattta atccaaaaaa aacgagacgg tcaagaactg
     3301 acttcaagtg aaattgaatg gctgatagaa ggctatgtat caggaactgt tcctgattat
     3361 cagatgtctg cctttgctat ggctgtttat tttaaaggaa tgacgactcg agaaatctct
     3421 gatttaacga tgaatatggt taagactggg caagagtttg acctgtcagc cattgatggt
     3481 gtgaaagttg acaagcattc tactggtggt gtcggcgata aggtaacctt gattttagct
     3541 cctcttgttg ctagcttcgg tgtgcctgta gcaaaaatga gtggtcgtgg tcttggccat
     3601 acaggcggaa caattgataa attggagtcc attaagggct atcaagtaga acgtagtcaa
     3661 gaggatttta ttcgtcaggt tcaggatatt ggagtatctg tcattgggca atcagaccag
     3721 ctggtcaaag cagataagct tctctatgcc cttcgtgatg tgaccgcaac tgtcgacacg
     3781 attcctttga ttgcgagttc ggtgatgagc aagaaaattg cggcaggggc ggatgctatt
     3841 ttgctagacg tgactgtcgg tgagggtgcc ttcatgaaga cggttgatga ggcgcgtgag
     3901 ttggctcaaa ctatggtaga tcttggtaag gttgttggac ggaagacagt agcagtgatt
     3961 acggatatga gccagccttt gggacgagcg attggaaatc gtctggaaat ccttgaagca
     4021 ttggagattt tgcaaggtca aggacgtcag gatattaccc actttatttg tgaattggct
     4081 caaattatgc ttggtttggc aaatgttaac aaaactgttg aagaagttca ccaacatctt
     4141 gagaatggtc aagcactggc taagtttgag gaaatggtgc aagcccaagg tggagacttg
     4201 gaagacctct atcgtcctgt aaatgtagct catgtggtgg aaatccctgc tcaggaaacg
     4261 ggtgtcattt cagctcttcc agctatggac tttggtcttt atgccatgag gctgggtgct
     4321 ggtcgtgcag ttaagtctga tgccttggac tatgaaacag gaattgtttt tgaaaagaag
     4381 attggagact ccgttcaaaa gggtgaaatt gttgcaaaag tatacacaaa tgaaaaaatt
     4441 ccacctcaac tagttacaga tttccaaaaa tgtgttaaaa taagtgatga agtgaaaaaa
     4501 atccgtgaaa ttgtagaaat catctcctaa atggagggaa aaccgaaatg aaattaaata
     4561 aatatataga tcatacgctt ttaaaacaag atgcaaagaa aaaacaaatt gatagtttgt
     4621 tgtctgaggc tagagagtat gactttgcca gtgtttgcgt taatccgacc tgggttgaac
     4681 atgctaaaaa aggacttgaa ggcacagatg ttaaggtttg cacagtagta ggtttccctt
     4741 tgggagcaac aacttcagcc gtgaaagcat ttgagacaaa agaagctatc caaaatggtg
     4801 cagatgagat tgatatggtg atcaatgttg gagctctcaa atcaggtaat ttagccttgg
     4861 ttgagtcaga tattcgcgca gtagtggaag caagtggtga taagttagtg aaagtcatta
     4921 ttgaagcttg ccttctgaca gaccaagaaa aagttgttgt ttgccaattg gcccaaaaag
     4981 ctggggctga ctttgtcaaa acatctactg gcttttcaac tggtggtgct acgatagcag
     5041 atgttacatt aatgcgtgaa acagttggat ctgatatggg tgtcaaggcc gccggtggag
     5101 ctcgttctta tgcagatgct cttgcctttg tcgaagcagg tgcgacccgt atcggaacgt
     5161 cagctggggt agctatttta aaaggagaat tggcagatgg cgactactga gttgattgaa
     5221 ctggcaattg aaaccagcaa acatgcctat gtcccctatt ctcactttcc tatcggagca
     5281 gttttagtag ctaaagacgg aagtgtttac acgggagtga atattgaaaa tgctagctat
     5341 cctttgacca attgcggtga gagaacagct atttttaagg ctatatctga aggccaaaga
     5401 gaattttcag aattgattgt ctatggtcaa actgaaaaac cgatctcgcc atgtggtgct
     5461 tgtcgccaag tgatggctga attttttgaa caagatctaa aagtgacctt agtcgcaaaa
     5521 gataaatcga cggtcgagat gacggtcggg gaattacttc catactcttt tacagactta
     5581 aactagtctg agtcgctctc tgagtggcac ggtccttgtg gccaaccaat ccatacttgc
     5641 aacatcgttg cacatcttat ttaggaggtt cagtaatgaa caagaaacaa tggctaggtc
     5701 ttggcctagt tgcagtggca gcagttggac ttgctgcatg tggtaaccgc tcttctcgta
     5761 acgcagcttc atcttctgat gtgaagacaa aagcagcaat cgtcactgat actggtggtg
     5821 ttgatgacaa atcattcaac caatcagctt gggaaggttt gcaggcttgg ggtaaagaac
     5881 acaatctttc aaaagataac ggtttcactt acttccaatc aacaagtgaa gctgactacg
     5941 ctaacaactt gcaacaagcg gctggaagtt acaacctaat cttcggtgtt ggttttgccc
     6001 ttaataatgc agttaaagat gcagcaaaag aacacactga cttgaactat gtcttgattg
     6061 atgatgtgat taaagaccaa aagaatgttg cgagcgtaac tttcgctgat aatgagtcag
     6121 gttaccttgc aggtgtggct gcagcaaaaa caactaagac aaaacaagtt ggttttgtag
     6181 gtggtatcga atctgaagtt atctctcgtt ttgaagcagg attcaaggct ggtgttgcgt
     6241 cagtagaccc atctatcaaa gtccaagttg actacgctgg ttcatttggt gatgcggcta
     6301 aaggtaaaac aattgcagcc gcacaatacg cagccggtgc agatattgtt taccaagtag
     6361 ctggtggtac aggtgcaggt gtctttgcag aggcaaaatc tctcaacgaa agccgtcctg
     6421 aaaatgaaaa agtttgggtt atcggtgttg atcgtgacca agaagcagaa ggtaaataca
     6481 cttctaaaga tggcaaagaa tcaaactttg ttcttgtatc tactttgaaa caagttggta
     6541 caactgtaaa agatatttct aacaaggcag aaagaggaga attccctggc ggtcaagtga
     6601 tcgtttactc attgaaggat aaaggggttg acttggcagt aacaaacctt tcagaagaag
     6661 gtaaaaaagc tgtcgaagat gcaaaagcta aaatccttga tggaagcgta aaagttcctg
     6721 aaaaataatg gatggaagtc atttcttagg aagcggccaa gggccgcttt tttaagagtt
     6781 gagacaaagt cattttgtct aagtaaagct tgctactaga caaactagca agttttattg
     6841 aaataaaata agactctgaa aggaagagca catggcacac gaaaatgtca ttgagatgcg
     6901 tgatattacc aaggtgtttg gtggatttgt tgccaacgac aaaatcaact tgcacctacg
     6961 aaaaggtgaa attcatgcac ttttaggaga aaatggggct ggtaagtcca cgctaatgaa
     7021 catgttagca ggccttcttg aaccaactag tggtgaaatc gcggtcaacg gtcaagttgt
     7081 caatctcgac tccccatcta aagcagctag cttgggaatc gggatggttc accagcactt
     7141 tatgttggtt gaagccttca cagtggctga aaacatcatt ttaggtagtg aattgactaa
     7201 aaatggtgtg ctagatatcg ctggagctag caaagaaatc aaggctcttt ctgaacgtta
     7261 tggcttagct gttgaccctt ctgccaaggt agcagatatc tcagttggag cccaacaacg
     7321 tgtagaaatt ttaaaaacac tttatcgggg ggctgatatc cttatctttg acgaaccaac
     7381 ggctgttttg actccatcag aaattgatga gttgatggct attatgaaaa atcttgtcaa
     7441 agaaggaaaa tcaattatct tgattaccca caaattggat gaaattcgag cagtttctga
     7501 ccgtgttaca gttatccgtc gtgggaaatc aattgaaacc gttgaaattg caggggctac
     7561 caatgctgat ttggcggaaa tgatggtagg acgttctgtt tcctttaaaa cagagaagca
     7621 agcctctaaa ccaaaagaag tggttttgtc tatcaaagat ttggtggtca atgaaaaccg
     7681 tggtgttcca gctgttaaaa atctgtcctt ggatgttcgt gctggagaga ttgttggtat
     7741 tgcggggatt gatggaaatg gtcagtctga actgattcaa gccattacag gtcttcgtaa
     7801 ggttgaatct ggtagcattg agctaaaagg agattcaatt gtaggcttgc acccacgtca
     7861 gattacagaa ctaagtgttg ggcacgttcc agaagaccgt caccgtgatg gcttgatttt
     7921 ggaaatgatg atatctgaaa atattgccct tcaaacctac tataaagaac cacatagtaa
     7981 aaatggaatt ttgaattatt caaatattac ttcttatgct aaaaagctga tggaagagtt
     8041 tgatgttcgc gctgccagtg aattagttcc tgcagctgca ctctcaggag gaaatcaaca
     8101 aaaagcaatt attgctcgtg aaattgatcg agatcctgat ctccttatcg ttagccagcc
     8161 aactcgtggg ttggatgtcg gtgccattga gtatatccac aaacgcttga ttgaagagcg
     8221 tgataatggc aaggctgtcc ttgttgtcag ctttgaattg gatgagattt taaacgtctc
     8281 agaccgtatt gccgttatcc acgatggtaa gattcaaggt attgtatcac cagaaacaac
     8341 caataaacaa gaacttggtg tcttgatggc tggtggaaac ttgggaaagg agaagagtga
     8401 tgtctaaaaa attacaacaa atttcggttc ccttgatttc tgtattccta ggaattttac
     8461 tcggagccat tgtcatgtgg atcttcggtt atgatgctat ttggggttac gaagaattgt
     8521 tctatacagc ctttggcagt ctgcgtggga ttggagaaat cttccgtgct atgggtcctc
     8581 tggtcttgat tggtcttggt tttgccgttg ccagtcgagc tggtttcttt aacgtcggac
     8641 ttcctggtca ggctttggca ggttggattc tcagtggttg gtttgccctg tcgcatccag
     8701 atatgccccg tcccttgatg attctagcaa ccatcgtgat tgccttgatt gctggtggga
     8761 ttgtcggagc gattccaggt attcttaggg cctatctagg gacgtcagag gttattgtaa
     8821 ccatcatgat gaactacatt gtcttgtatg tagggaatgc ctttatccat gctttcccta
     8881 aagacttcat gcaaagtaca gattcgacca ttcgtgttgg ggctaatgca acctatcaga
     8941 caccttggtt ggctgagttg actggtaact cacggatgaa tattggtatt ttctttgcca
     9001 tcattgccgt tgcagttatt tggttcatgc tcaagaaaac aactcttggt tttgaaatcc
     9061 gtgcagttgg tcttaatcca catgcttcag aatatgctgg tatttctgcc aagcggacta
     9121 ttatcctatc tatgattatt tcaggtgcct tggcaggtct tggtggagct gttgaaggtt
     9181 tgggaacctt ccagaacgtc tatgttcaag gttcgtcatt agctatcgga tttaacggaa
     9241 tggcggttag tttgcttgcg gccaactcac caattggtat actctttgca gccttcctat
     9301 ttggcgttct ccaagttggg gctcctggta tgaatgcggc gcaggtacca tctgagcttg
     9361 tcagcattgt aacagcgtct attatcttct ttgtcagtgt tcattacctt atcgaacgct
     9421 ttgtcaaacc gaaaaaacaa gttaaaggag gtaagtaaag atgtctatta taaccttgct
     9481 ccccctcttg gtgtcttcta tgctgattta ctcagcacct ctcatcttta caagtatcgg
     9541 tggtgttttc tctgaacgtg gtggtgtggt aaacgttggc cttgaaggaa ttatggttat
     9601 gggtgccttt tctggagttg tctttaacct tgaatttgca gaacaatttg gagcagcaac
     9661 tccatggcta tccttgcttg tagcaggatt ggttggtagt gttttctcta tcatccacgc
     9721 agcagcgacg gttcatttcc gtgcagacca tgttgtcagc ggtacggtat tgaacttgat
     9781 ggcgcctgcc ttggctgttt tcttagttaa agttctttat aacaaaggac aaaccgacaa
     9841 cctaagtcaa acttttggac gctttgattt cccagtcttg gcaaatatcc cagtgattgg
     9901 tgatatcttc ttcaagtcaa ctagtctact tggttatctg gcgattgcct tctcattcct
     9961 tgcttggttt attctcttta agactcaatt tggtcttcgt ctccgctctg tcggtgaaca
    10021 tcctcaagca gcggatacct tgggaatcaa cgtctacaag atgagatatt taggggttat
    10081 tatttcaggt tttctaggtg gaattggcgg agcgatttat gcgcaatcaa tctcagttaa
    10141 cttctcagtg acaactattg ttggacctgg atttatcgcc cttgctgcga tgatctttgg
    10201 gaaatggaat ccaatcggtg ctatgctttc tagtctcttc tttggacttt cacaaagttt
    10261 ggctgttatc ggttctcaat tgccgttcct acaaggagtg ccagcggttt atcttcaaat
    10321 tgcaccttat gttttgacaa ttcttgtctt agcagccttc tttggaaaag cagtcgcacc
    10381 aaaagcagat ggtatcaact acatcaaatc aaaataagca tacaaaaaaa cgtcagtcca
    10441 atgactgaac tacaccccaa aagttagaca gaaaaaatct aacttttggg gtgtttttat
    10501 tatgaaatta agttatgatg ataaagttca gatctatgaa cttagaaaac aaggatatag
    10561 cttagagaag ctttcaaata aatttgggat aaacaattct aatcttaggt atatgattaa
    10621 attgattgat cgttacggaa tagag
//
LOCUS       DBDESG010000069        10053 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.069, whole genome shotgun sequence.
ACCESSION   DBDESG010000069 DBDESG010000000
VERSION     DBDESG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.069"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..141)
                     /locus_tag="ACKB38_001933"
                     /pseudo
     CDS             complement(1..141)
                     /locus_tag="ACKB38_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            554..649
                     /locus_tag="ACKB38_001934"
                     /pseudo
     CDS             554..649
                     /locus_tag="ACKB38_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(902..1522)
                     /locus_tag="ACKB38_001935"
     CDS             complement(902..1522)
                     /locus_tag="ACKB38_001935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGL7980643.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(1522..2004)
                     /locus_tag="ACKB38_001936"
     CDS             complement(1522..2004)
                     /locus_tag="ACKB38_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGL7980644.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(2004..2846)
                     /locus_tag="ACKB38_001937"
     CDS             complement(2004..2846)
                     /locus_tag="ACKB38_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGL7980645.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLIDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2941..3741)
                     /locus_tag="ACKB38_001938"
     CDS             complement(2941..3741)
                     /locus_tag="ACKB38_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGL7980646.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3885..5264
                     /gene="glmU"
                     /locus_tag="ACKB38_001939"
     CDS             3885..5264
                     /gene="glmU"
                     /locus_tag="ACKB38_001939"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGL7980647.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            5274..5819
                     /locus_tag="ACKB38_001940"
     CDS             5274..5819
                     /locus_tag="ACKB38_001940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGL7980648.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5831..6145
                     /gene="macP"
                     /locus_tag="ACKB38_001941"
     CDS             5831..6145
                     /gene="macP"
                     /locus_tag="ACKB38_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGL7980649.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            6162..6854
                     /locus_tag="ACKB38_001942"
     CDS             6162..6854
                     /locus_tag="ACKB38_001942"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGL7980650.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6931..7422
                     /gene="rocS"
                     /locus_tag="ACKB38_001943"
     CDS             6931..7422
                     /gene="rocS"
                     /locus_tag="ACKB38_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGL7980651.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7520..8110
                     /locus_tag="ACKB38_001944"
     CDS             7520..8110
                     /locus_tag="ACKB38_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGL7980652.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            8119..9882
                     /locus_tag="ACKB38_001945"
     CDS             8119..9882
                     /locus_tag="ACKB38_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGL7980653.1"
                     /translation="MKPEKPKKLGLKQIYLDLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3098 a         1744 c         2125 g         3086 t
ORIGIN      
        1 gttgccctta cgtgacatgg atgcttgaat tcccttactc tctaggaagc gatgataaga
       61 atcgtgttga tattgccagc cttggtcact atggagaatc gtattctcgt agtgcttctc
      121 tttgaatgcc tgttccaaca ttaacgatca atcaatttaa tcatgtacct aagattagaa
      181 ttgtttatcc caaatttatt tgaaagcttc tctaagctat atccttgttt tctaagttca
      241 tagatctgaa ctttatcatc ataagttaat ttcataataa aaacacccca aaagttagat
      301 tttttctgtc taacttttgg ggtgtagttc atgtacacct gatatgatgc gttttataat
      361 tttaaagact ttttgaccag cctcattttt ttaacttgat actcagtgaa aagcaaagat
      421 taaactagga agctagctgt aggctgctca aagaacagct ttgaggttgt agataaaact
      481 tgtgaggtca ccaacatata taatgtgaag ctgacgtggt ttgaatagat tttagaagag
      541 tatgagtctg gaagttttaa tggataatgc aagattccat agaatgggta agctagagtt
      601 cttatgtgaa gagtttgggc ataaactttt accttttcct ccctactcat cttagtatag
      661 aaaagtgaat ctgaaatagt acataactgc ttctaaaaca ttcttataaa ttgatttaaa
      721 ttctcaaatc atattattca gttcttattt cattttgttc tacaatcctg ttgagaagac
      781 acgtgttcat atcaaaaagg tattggcaag ttgcaatacc tttttacgag gctctgttgt
      841 cttatttttg tttcaactga ctatatctcc tatggttcta gttcagaagg ctaggctata
      901 attatgattg ataagaagta tcattccaag tattgggagt gaatgtttca aaatcatggg
      961 tttctataat ggtcaggctg gcatttgcta gaccgccatc tttacgaaga agtggttctt
     1021 tatagcctag gagagtacga agactggcag taagattggc gccgtgtccg acaattagaa
     1081 tacgttcagc tggactatct tttaatgatt tgataaattg gatggtccgt tgagttgtac
     1141 tatagaggga ttcggctccg aacattcgag tgtcaaattg agcaagattt gaacgaaaag
     1201 cctggatttg ttgcgggtaa atagcttcca aggttgcaat tttcaaacct tctaacttcc
     1261 caagttgcca ttcacggaga ttaggaacga tttctaaaga acagggggta tagagttgac
     1321 tttggataat ctcagcagat ttgaccgctc gaggtaaatc acttgaataa atctgatcaa
     1381 aaggaatttc cttgagatac tgaccaagtc gttttagggt ttcaatggat tcaggaagaa
     1441 gaggagaatc accactagca ccttgaaaac gaccttcttg gttccagagg gtacgaccgt
     1501 ggcggacaaa gtagagtttc attacttgat gtcctccaaa atatctacaa agtctgcctt
     1561 tacaaagcta gccaagtctt gtggcgcgac gataatgctg tgtccgactt cgcctgcaga
     1621 gacaatcatt tgatccaaat ctagagcaat tttatcgata aaaatgggat aattgtgttt
     1681 ctgacgaatt ccgacaggat tattggctcc atgaatgtaa ccagttgttt tttctaagtc
     1741 cttttgtgga atcatgctca cttttttatt gccagaaatt ttagctagtt tcttttcaga
     1801 caagtgctga gtgataggga caattccgat aatcggtccg gtcttgtctc ccaaaagcgc
     1861 caaggttttg aaaatctgat ctcgttcata accttgagga agctctcctt ctagggcatt
     1921 gatttgaatc ccctgatgag ggatagctgc tttagatagg atttgttcca ccaatgtttt
     1981 tttgatttta actttttttg ccattattta tatttatcct ccaattgact catccaaata
     2041 ccaagccaga ttcccagcgc aaagaagaag gcgatgatga cataaccgac aagtgaaagt
     2101 cctgtgtatt ggatactttc agcgtttcct gcatttggaa ttaagatcaa aagggtactt
     2161 gataggacga taccgatgat gaaatgatag acgcgtgagt ggtagttgtt taaggcataa
     2221 tctatcaatt ttgaaaaaac gatgagagtt gcacctgcac caattccaat cggaaagaag
     2281 gttcccaaga aatcaaaagt tttaaaacca gtcaacatag gagcatagag tcccaaaatc
     2341 aaaagtaaat ttgatgggct gaggccagga accaagacgc caagggccaa tagtgcgcct
     2401 gctaggacga agttaagaaa gctggcgctt aaggttccaa cgacaaaatt taaggcatag
     2461 agtcctaatc cagaaatgat aaaggttgtc cataaccaag ctaaatcaat cttgtctcgg
     2521 tcagattctc gagttgattc tttgaggagg ctaggaactg taccgataat agctcccgca
     2581 aagctccata atacaaaaac ctgataattt tcaagcaggt attcaatcgg gtaggaaaat
     2641 aagccgattc ccagaagcat accgatggca actggaataa agtacaaaac attttcttta
     2701 aagtctttaa agggatgggc cagaaagcca atcattcgtt catagattcc taagattgct
     2761 gctagaaccc ctccggaaat tcccggtagg ataaatccaa gagcaattac aatcccttta
     2821 ataacgcgtg ctaaccatga gagcatatat ttcctccaac tatttctatt tcaaaaaatc
     2881 cttactatat tgtatcacaa tcagacacaa aaagaaaaag caaatgataa acaaatgctt
     2941 ttaaggtttt aaaaaaagtt ttcgaaaggt ttcttcttta ttttttaagg gagagataac
     3001 gttgatatct aaatcgtggt caaagccggc aatttttcct ttagatgtgt attggtgaat
     3061 atcataatct aaatcagttt taggactgct ctccaaaaat cctgagtctg agccgtagga
     3121 cggaatccaa acagaggtaa acttgcctgt atcaatactg tgttcttcca tgaagtagac
     3181 accaacgtag atgccgatgt ttttagcacc cagtgatgct agttttgctc gaaagttttc
     3241 gacaccttcg ttcatattag acatggtttt gtcttccacg tcaagccaat agtaactagg
     3301 gctgtaagga gaggcagcat tgtagaaaac ttcggcagcc ttttccattt cttggacact
     3361 ttttccagct acataagcgt agacagcaac tgggacattc cgcttttgaa gttcagtgat
     3421 atgactctta taggccttgt ctattccatt gataaatgaa gcatcatttt cttttgtcgt
     3481 ttgagcacca ctgtgaacac gaacaatagc acctgaaata ttttgtgaga gggcatcgta
     3541 gttgatttcc tcaggacgct gccagccaga gaggtcaata atcggtttgt ctaagtgttt
     3601 caaagcctgt gcttcaatct gtgctatatt ggattttgtt ttaaacgatt ggctgtcatt
     3661 aagtgggcga ttgatgatta aaatgaacat cataatccca aaaaaactaa ataaaataag
     3721 tggatgaatt tgttttctca tatcttataa ttctacccta aaaatcaaaa aaaatcaaaa
     3781 aaatgggtta aggaagagac tttagagcat tttttcattc aagagtgcgg aatgatttga
     3841 aatatggtat aataaaaggg aatttctaca gaaaagagaa gattatgtca aattttgcca
     3901 ttattttagc agcgggtaaa gggactcgca tgaaatctga tttgccaaaa gttttgcaca
     3961 aggttgcggg tatttctatg ttggaacatg ttttccgtag tgtgggagct atccaacctg
     4021 aaaagacagt aacagttgta ggacacaagg cagaattggt tgaggaggtc ttggctggac
     4081 agacagaatt tgtgactcaa tctgaacagt tgggaactgg tcatgcagtt atgatgacag
     4141 agcctatctt agaaggtttg tcaggacaca ccttggtcat tgcaggagat actcctttaa
     4201 tcactggtga aagcttgaaa aacttgattg atttccatat caatcataaa aatgtggcca
     4261 ctatcttgac tgctgaaacg gataatcctt ttggttatgg acgaattgtt cgtaatgaca
     4321 atgctgaggt tcttcgtatt gttgagcaga aggatgctac agattttgaa aagcaaatca
     4381 aggaaatcaa cactggaaca tacgtctttg acaacgagcg tttgtttgag gctttgaaaa
     4441 atatcaatac caataacgct caaggcgaat actatattac agacgtcatt ggtattttcc
     4501 gtgaaactgg tgaaaaagtt ggcgcttata ctttgaaaga ttttgatgaa agtcttgggg
     4561 taaatgaccg tgtggcgctt gcgacagctg agtcagttat gcgtcgtcgc atcaatcata
     4621 aacacatggt caacggtgtt agctttgtca atccagaagc aacttatatc gatattgatg
     4681 ttgagattgc ttcggaagtt caaatcgaag ccaatgttac cttgaaaggg caaacgaaaa
     4741 ttggtgctga gactgttttg acaaacggta cttatgtagt ggacagcact atcggagcag
     4801 gagcggtcat taccaattct atgattgagg aaagtagtgt tgcagacggt gtgatagtcg
     4861 gtccttatgc tcacattcgt ccaaattcaa gtctgggtgc ccaagttcat attggtaact
     4921 ttgttgaggt gaaaggatct tcaatcggtg agaataccaa ggctggtcat ttgacttata
     4981 tcggaaactg tgaagtggga agcaacgtta atttcggtgc tggaactatt acagtcaact
     5041 atgacggcaa aaacaaatac aagacagtca ttggaaacaa tgtctttgtt ggttcaaatt
     5101 caaccattat tgcaccagta gaacttggtg acaattccct cgttggtgct ggttcaacta
     5161 ttactaaaga cgtgccagca gatgctattg ctattggtcg cggtcgtcag atcaataaag
     5221 acgaatatgc aacacgtctt cctcatcatc ctaagaacca gtaggagcct atcatggagt
     5281 ttgaagaaaa aacgcttagc cgaaaagaaa tctatcaagg accaatattt aaactggtcc
     5341 aagatcaggt tgaattacca gaaggcaagg gaactgccca acgggatttg attttccaca
     5401 atggggctgt ctgtgtttta gcagtaacgg atgaacaaaa acttatcttg gtcaagcagt
     5461 accgcaaagc tatcgaggct gtctcttacg aaattccagc cggaaaattg gaagtaggag
     5521 aaaacacagc ccctgtggca gctgcccttc gtgaattaga ggaagaaaca gcctatacag
     5581 ggaaattaga actcttgtac gatttttatt cagctattgg cttttgtaat gagaagttaa
     5641 aactatattt agcaagcgat ttgacaaaag tggaaaatcc gcgtccgcag gatgaggatg
     5701 aaaccttgga agtccttgaa gtgagcttag aagaagcgaa agaattaatc caatcaggtc
     5761 atatctgtga tgccaagaca attatggctg ttcagtattg ggagttgcag aaaaaataga
     5821 ggaggtcagt atgggtaaat ctttattaac ggatgaaatg attgaaagag ctaatagagg
     5881 cgaaaaaatt tcaggtcctc ctttgctaga tgataatgag gaaactaaga ttttaccaac
     5941 ctcttcttcc cgttttggtt atgccaatcc taaggatcat ggttttagcc aggaaacctt
     6001 gaagattcag gtcgaaccat ctattcataa aagccgtcgt attgaaaata ccaagagaaa
     6061 tgtcttcaat tctaagttga ataaaatctt atttgcggtc atctttctct tgattttgct
     6121 tgttttagca atgaaacttt tgtaatagaa aaggaattga aatgaaaata ggaattattg
     6181 ctgctatgcc agaagaactg gcttatctgg tccagcattt agataatgcc caggagcaag
     6241 ttgtttttgg gaatacctat catacaggaa ccattgcttc tcatgaagtc gttcttgtag
     6301 aaagtggaat tggtaaggtc atgtctgcta tgagtgtggc gattttggct gatcatttcc
     6361 aggtggatgc ccttattaat acgggttcag ctggggcagt agcagaaggt atcgctgttg
     6421 gggatgtcgt gattgctgac aaattagcct atcatgacgt ggatgtcaca gcttttggct
     6481 atgcttatgg acaaatggcg caacaaccgc tttatttcga atcagacaaa acctttgttg
     6541 ctcaaatcca aaagagttta tctcaattgg accaaaactg gcatcttggt ttgattgcta
     6601 caggagatag ttttgttgca ggaaatgaca agatagaagc gattaagtcc catttcccag
     6661 aagttttagc cgtggagatg gagggggcag ctattgctca agcagcgcat gccctcaatc
     6721 tcccagtctt agtcatccga gctatgagtg acaatgccaa ccatgaagca aacatctttt
     6781 ttgatgagtt tattatcgaa gctggacgtc gctctgccca agtcttgttg acctttttga
     6841 aggctttaga ttaagcggaa atttgacagt ttttctagct tatgataaga tttaagtaaa
     6901 gaaaagctag aaaacgtttc agaggatatt atgagtattg aaatgaccgt cagtgagatt
     6961 gcagaggtct taggattatc tcgccaagca atcaataacc gtgtcaaaga attaccagaa
     7021 gaagacacag ataaaaatga caaaggggta acagttgtta ccagaagtgg cttgattaag
     7081 ctagaagaaa tctataaaaa aacgattttt gaagatgagc ctgtcagtga agatgtcaaa
     7141 caacgtgaac tgatggagat tctagtggat gagaagaatg cagaaattct tcgtctttat
     7201 gaacaattaa aagccaagga tcgtcagtta tcagaaaaag acgagcagat gcgtatcaaa
     7261 gaccgtcaga ttgctgagaa agacaaacaa ttagatcagc aacaacaatt gacccttcaa
     7321 gccatgaagg atcaagaaaa tcttaagcta gagctggacc aagcaaaaga agaagtccaa
     7381 tccactaaaa aaggcttttt tgctcgttta tttggaggat aatcaaggag ctgtttaggt
     7441 taaatgctaa cctgatggct tcttttgttt gtgaatagta tagttgatta gaacagagac
     7501 agtagaggag aggtacagaa tggaacgatt agaagattac attgcttttg atttagaatt
     7561 taatcagcac gaaggacaaa cacacttgat tcaagtatcg gcagtgcgtt ttagagatgg
     7621 tcgggaaatc gatgcctatg attcctatgt tcataccagc gtccctctaa aaagctttat
     7681 caatggtttg accgggatta cagctgagac cttaaaagat gctcctccag ttgagaaagt
     7741 cttagaagaa ttccaagagt ttgtgggttc tttgctgatg ataggctaca atgctgctaa
     7801 aagtgattta cctattttag cagagcatgg tttagactat agccaacagt acaaggtgga
     7861 tttgtatgat gaagcttttg aacgtcggag ttcagattta catggtattg ccaatctcaa
     7921 attgcaaact gttgcctcat ttcttggatt tcaaggtcag tctcataata gtttagagga
     7981 tgctcggatg acggcgcgtg tttacgaatc cttcttagag tcggatcaag ctagggaatt
     8041 gttagagaca gaaagtagct tatcaaacaa tccttttggt ggcttggact tgtctcaatt
     8101 atttgactag gagtgcctat gaaacctgaa aaacctaaaa aattgggtct taaacagatt
     8161 tacctcgatc ttgataaaat cctctttcta tttttcttga tttttatgat agtggagtat
     8221 ttgtgggtac cattcaattc atggttggct agcttcttat tagatcagac aggttatctt
     8281 tttatttcct ataacaatgt cttagccatc cttactcatt ctccattgat tagtctagct
     8341 tttttagcct tgattgtaat taaccttctg gttgcttatt ttcaaatagg actcctcttt
     8401 attggtgctc gtcatctcct ctataatgaa aagcgaaggc taattgagta tatccgcaag
     8461 gttttccatg aaagttttgg atttatgaaa aagctaactt gcgctaaagc cttgtttatc
     8521 tttttctata tagctatgct gtttcctttt atacggaaaa tgctgaagat ttattatctt
     8581 aataaaatcg tcattccaga gtttatccaa gcttatttag aagatagata ttggatgtgg
     8641 tggctgagca tcctacttct atctcttatc tttctctatg tctctgtcag attgatgttt
     8701 gcccttccga agattgtata tgatcagctg actgttcgag aagcagtgat gtttagcttg
     8761 gagaaaacca agaagagagt tactttttat gcttggaatt tattttacat cttgctcaaa
     8821 gcacatttat tattttatct tcctttgatt cctttattat tggctcaaac tttggtagat
     8881 gatatcactc agagagagtc tctgattctt ggtattttga attttgtcgt gattaaaaat
     8941 ctctattata tggctctgac ttattttttg gttaaatttg tttcattttt gacaggaaag
     9001 gagctggata tgcttcctag gagagaaaaa gatcacatcg tgcgatgggg tgtcatgact
     9061 tgtgccagtc ttttctttgc cttagaaggt tatatttatc tggaggctcc catggttcat
     9121 ctacctcaac ttatttctca ccgaggggtt tccaatgcaa atgggattca aaatacagta
     9181 gagtccttgg aaactacagc acaactcaaa ccagacttgg tggagacgga cgtgcaggaa
     9241 acaaaagatg ggcagtttgt catgatgcat gatgctaact tgaaaaatct agcaggtatc
     9301 aataaaagtc ctcaagactt aaacttggag gagcttaaag ggattgatat ttttgaaaat
     9361 ggctaccaga ctaaaatttc aagctttgaa gattatctca gtcgagccaa cgaacttggt
     9421 caaaaattac taattgaaat taaaaccagt aaaaaagata gtccagacat gatgaaccgc
     9481 tttttagccc gttatgctgc aaagctcaag atttatggac atcaaatcca gtctttagac
     9541 taccatgttg tcgaaaaagt aagacagtat gatgccgaac taccagttta tttcatcatg
     9601 ccctacaatt ctgtctttcc taaaacaaga gcgacaggat atactatgga gtactcaacc
     9661 ttggatgaat attttgtaag caaactatgg acaacggatc agaaacttta tgtctggact
     9721 gtcaatgatt cagaagctat cagcaaatct cttcacttag gagtagatgg agtgattaca
     9781 gatgatttgg aaaaagttca gcgggaaata gaagtagccc aagaagatcc agaatatacg
     9841 gatttgctct tgaaaaaagc attggaattc tttgaatttt agtaataaaa aagaggaacg
     9901 gttgttcctc ccatgatagg taagtattct tgtgaatgct tgcttttttg tggttgcaat
     9961 ccgtttacga gaattgtttg tattgttctt gtttcaattt actttatagt gctttgaagt
    10021 tggaatgcta cactataact tataaaatat agt
//
LOCUS       DBDESG010000070         9736 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.070, whole genome shotgun sequence.
ACCESSION   DBDESG010000070 DBDESG010000000
VERSION     DBDESG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.070"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..164
                     /locus_tag="ACKB38_001946"
     CDS             <1..164
                     /locus_tag="ACKB38_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HGL7980654.1"
                     /translation="GDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYG
                     VNANGEWVN"
     gene            complement(394..1374)
                     /gene="dusB"
                     /locus_tag="ACKB38_001947"
     CDS             complement(394..1374)
                     /gene="dusB"
                     /locus_tag="ACKB38_001947"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGL7980655.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIETLLQLEKA"
     gene            complement(1361..2233)
                     /gene="hslO"
                     /locus_tag="ACKB38_001948"
     CDS             complement(1361..2233)
                     /gene="hslO"
                     /locus_tag="ACKB38_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGL7980656.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2398..3825
                     /locus_tag="ACKB38_001949"
     CDS             2398..3825
                     /locus_tag="ACKB38_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980657.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIHYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            3984..5492
                     /gene="glpK"
                     /locus_tag="ACKB38_001950"
     CDS             3984..5492
                     /gene="glpK"
                     /locus_tag="ACKB38_001950"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGL7980658.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5534..7360
                     /gene="glpO"
                     /locus_tag="ACKB38_001951"
     CDS             5534..7360
                     /gene="glpO"
                     /locus_tag="ACKB38_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGL7980659.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7430..8134
                     /locus_tag="ACKB38_001952"
     CDS             7430..8134
                     /locus_tag="ACKB38_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGL7980660.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8431..8634
                     /locus_tag="ACKB38_001953"
     CDS             8431..8634
                     /locus_tag="ACKB38_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980661.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(8721..9308)
                     /locus_tag="ACKB38_001954"
     CDS             complement(8721..9308)
                     /locus_tag="ACKB38_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980662.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            9462..9659
                     /locus_tag="ACKB38_001955"
     CDS             9462..9659
                     /locus_tag="ACKB38_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980663.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNNKK"
BASE COUNT         2814 a         1931 c         2207 g         2784 t
ORIGIN      
        1 atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaagc caatggttca
       61 aagtatcaga taaatggtac tatgtcaatg gctcaggtgc ccttgcagtc aacacaactg
      121 tagatggcta tggagtcaat gccaatggtg aatgggtaaa ctaaacctaa tataactagt
      181 taatactgac ttcctgtaag aactctttaa agtattccct acaaatacca tatcctttca
      241 gtagataata tacccttgta ggaagtttag attaaaaaat aactctgtaa tctctagccg
      301 gatttatagc gctagagact acggagtttt tttgatgagg aaagaatggc ggcattcaag
      361 aggctcttta agagagttac gggttttaaa ctattaagcc ttctccaatt gcaagagggt
      421 ttcaatctct gccagggtgc tggcttgcga aatggctcca cggagtttgg cagcgccaga
      481 tgttccacgg agatagtgag gagcgagacc gcggaattca cgaactgcga cgttttctcc
      541 tttgaggtta atcaatcgtt tcaagtgttc gtaggcgatc ttcatcttgt cttcaaaggt
      601 caaatcaggt aggatttctc ctgtttcaaa gtaatggttg atttggttga agaggtaagg
      661 atttcccatg gcagctcggc caatcatgac tgcgtcagca ccaacttctt cgatgcgttg
      721 cttggcttct tggacagtac ggatatcacc gttggcgatg aatggaatct tggttagagc
      781 ttgggcaacc ttgtaaaggg tctcaaggtc tgcgtggcca gtatacattt gttcacgggt
      841 acggccatgc atggcgaggg cagaaacacc tgcagcttca gcagcgagag cattttctac
      901 tgcaagagat gggtccgccc agccggtacg cattttgaca gtaagtggga tatcaaggac
      961 agactggacc ttgttgatga tggagtaaat cttgtctgga tccttgagcc acatagcacc
     1021 agcttcgttc ttcacgattt tgttgacagg gcagcccatg ttgatatcga cgatatcggt
     1081 cttggtgttt tcttggatga attctgctgc gcgtgctagg ctgtcttcat cgctaccaaa
     1141 aagttggata gagacagggt tttcgccctc atcgatatga agcatgtgca gggttttttc
     1201 gttgttgtat tggattccct tgtcagagac catttccatt acaacgagtc cagctccgag
     1261 ctcctttgcg atagtacgaa aggctgagtt ggtcacgcca gccataggcg ctaaaacggt
     1321 acgattggga atctcaatat tgccaatcat aaaaggtgta ttaagatttg tcacgaatga
     1381 gttcctccag gtccttttca tcaaagttgt aagtagtttg gcagaattga caagtgattt
     1441 ctgccccgtg gtcttcctct ttcatttcct gtaagtctga gcttggaagg ctggcaagag
     1501 cgttcataaa gcgttcatgg ctacagtcac attggaaacg gatttcttct tcagaaagac
     1561 gcttgtaggc ttcgtccccg tagatagcct tgaggagggc ttcgatatgg tcgtcgcttt
     1621 cgagaagagt agagatagct ggcatttctt ggatgcgttt ttcaaagcga gcaatctctt
     1681 ctttcttggc tcctggcaag acttgaacta ggaaaccacc tgcaaccttg accttgtctt
     1741 cctcgtccaa aaggacattg aggccgaccg ctgaaggcgt ttgttggctt tcagtaaggt
     1801 aaaaggcaag gtcttcaccg atttctccag agatgagggg agttatagag ttgtaaggat
     1861 ttccagtacc gtagtctgtg ataacgagga attgaccatt tccaacaaaa ggtccgacta
     1921 ggacttcacc agtcgcagtc tttttgatgt caacaccagg attttgaaca tagcctttga
     1981 cgttcccctt ggtatcagcg acggtgataa tagcacctag agagctagat cccaacacct
     2041 taactgtaag tttggtattt cctttttcat tggctgcgag aatctggcta gcgataagag
     2101 ttcgaccaag cgctacagtt gagctagctt gggtttgatg tttttcttga gcagtgcgga
     2161 cggtttcagt gctatcaagg acaaaagcac gaaaggctcc gctttctgat atagttttaa
     2221 taattttatc catagctact attttagcat aaaaatgccc aaagggggag ccgtgtgttt
     2281 actgattttc aggataatgg accaggaaat cagcatgaaa ataaaaagag aaacagatta
     2341 ttttagcatt tgtcagattt atgctatgct taaggtagaa aatgaaaggg ataacaaatg
     2401 tatttaggag atttgatgga gaaagccgag tgtggtcaat tttcaatact ttcctttcta
     2461 ttacaagagt ctcagacgac cgtcaaggct gtaatggaag aaacaggatt ttcaaaagca
     2521 accctaacca aatatgtcac cctgctcaat gacaaggctt tggatagtgg cttagagctg
     2581 gctattcact cagaagatga aaatctgcgt ctgtctatcg gtgcagctac caaggggaga
     2641 gatattcgga gcttgttttt ggagagtgct gttaaatacc agattttggt ttatcttctc
     2701 taccaccaac agtttttagc ccatcagctg gctcaagaat tggtgattag cgaggctacg
     2761 cttggtcgtc acttggctgg tttaaatcag attttgtcag aatttgattt atccatccaa
     2821 aatggccgtt ggcgaggtcc agagcatcag attcactatt tctatttctg tcttttccga
     2881 aaggtctggt cgagtcagga atgggaaggt cacatgcaga aaccagagag aaaacaggag
     2941 attgccaatt tagaggaaat ctgcggtgca agtttgtctg cggggcagaa attggacttg
     3001 gttctctggg ctcacatcag tcaacaacgt cttcgggtca atgcttgtca gtttcaagtc
     3061 atagaagaga aaatgcgagg gtattttgac aatatctttt atcttcgttt gctgagaaag
     3121 gttccgtcct tttttgctgg gcaacatatt ccactaggag ttgaggatgg tgagatgatg
     3181 atattcttct cttttctcct atctcatcgc attcttcctc ttcatactat ggagtatatt
     3241 cttggttttg gagggcagtt ggcagattta ctgacgcaat tgattcaaga aatgaagaag
     3301 gaggaactat tgggggatta tacagaggac catgtcacct atgaactcag tcagctttgt
     3361 gctcaagtct atctctataa gggctatatt ttacaggatc gctacaagta ccagttagag
     3421 aatcgtcatc catatttact gatggaacat gattttaaag agacagcaga ggagattttt
     3481 catgctctac ctgcttttca acaggggaca gatttagata agaagattct ctgggaatgg
     3541 ctccagttaa tcgaatatat ggctgaaaac ggtggccagc atatgcggat tggtctggat
     3601 ttgacatctg gttttcttgt cttttcaagg atggcagcca ttttgaaacg gtatttggaa
     3661 tacaatcgtt ttattaccat tgaagcttat gaccctagtc ggcattatga tttgctggtt
     3721 accaataacc cgattcataa gaaggaacag acaccagtct attatttaaa aaatgacttg
     3781 gatatggagg atttggtagc gattcgccag ttattattca cttaaaaggc ttggttaatc
     3841 caggtctttt ttgtgaaatt cacacaatct cctcacattt ttttaaaaat taaaaaaagt
     3901 tgataaacaa gaaagcgctt tattttgtat actagtaagt gtaaagagga aacacctcaa
     3961 gatctttatc aggaggacag tacatgtcac aagaaaaata catcatggcc attgaccagg
     4021 gaactacaag ttctcgtgcc atcattttca acaaaaaagg ggaaaaggtt agctcgagtc
     4081 aaaaagagtt tacccagatt ttccctcagg caggttgggt tgagcacaat gccaatgaaa
     4141 tttggaactc tgttcagtca gttattgcgg gtgctttcat cgaaagtggt gtcaagccaa
     4201 atcaaatcga ggcaatcggg attaccaacc aacgtgaaac aacggttgtc tgggataaga
     4261 aaacaggact tcctatctac aatgctatcg tttggcagtc acgccagaca gcacctttgg
     4321 ctgagcaact aaaaagccaa ggttatgtgg aaaaattcca tgaaaagact ggtttgatta
     4381 ttgatgctta cttctctgct accaaggttc gttggatttt ggatcatgta gaaggtgctc
     4441 aagagcgagc agaaaaaggg gaattgctct ttggtactat cgatacttgg ttggtttgga
     4501 aattgactga cggtgcggct cacgtgactg actactcaaa tgcagctcgt accatgcttt
     4561 ataacattaa agaactcaaa tgggatgatg agattttgga aatccttaac attccgaagg
     4621 ctatacttcc agaagttcgt tctaactccg aaatctacgg caagacagct ccattccatt
     4681 tctacggtgg agaggtgcca atctcaggta tggctgggga ccaacaagca gccctctttg
     4741 gacagttggc ttttgagcca ggtatggtta agaatactta tggaacaggc tctttcatca
     4801 tcatgaatac tggggaagag atgcagttgt ctgaaaacaa cctcttgaca accattggtt
     4861 acggaatcaa cggtaaggtt tattatgcct tggaaggttc tatcttcatc gcaggaagtg
     4921 ctattcagtg gcttcgtgac ggtcttcgca tggttgaaaa ttcaccagaa tctgaaaaat
     4981 acgctcgtga ttctcacaac aacgatgaag tttatgtcgt tccagccttt acaggtctag
     5041 gcgctccata ctggaaccaa aatgctcgtg gttccgtctt tggtttgact cgtggaacaa
     5101 gcaaagaaga ctttatcaag gcgactttgc aatctattgc ttatcaagtg cgtgatatca
     5161 tcgacaccat gcaagtggat actcagaccg ccattcaagt actgaaggtg gatggtggtg
     5221 cagccatgaa caacttcctc atgcagttcc aggcggatat tttaggcatt gacattgcac
     5281 gtgctaaaaa cctggaaaca acagctctag gagcggcctt cctagcaggt ttgtcagtag
     5341 ggtactggaa agacttggac gagttgaaac tcttgaacga gacaggagaa ctctttgagc
     5401 catctatgaa cgaatctcgc aaggaacaac tctacaaggg ctggaagaag gctgtgaaag
     5461 caactcaagt ctttgcggaa gtagacgact aatactggca gaataaagcg atttatttag
     5521 aaagtgtgta aatatggaat tttcaaagaa aacacgtgaa ttgtcaatta aaaaaatgca
     5581 ggaacgtacc ctggacctct tgattatcgg tggaggaatc acaggagctg gtgtagcctt
     5641 gcaggcggca gctagcggtc ttgagactgg tttgattgaa atgcaagact ttgcagaagg
     5701 aacatctagt cgttcaacaa aattggttca cggaggactt cgttacctca aacaatttga
     5761 cgtagaagtg gtctcagata cggtttctga acgtgcagtg gttcaacaaa tcgctccaca
     5821 cattccaaaa tcagatccaa tgctcttacc agtttacgat gaagatggag caacctttag
     5881 cctcttccgt cttaaagtag ccatggactt gtacgacctc ttggcaggtg ttagcaacac
     5941 accagctgcg aacaaggttt tgagcaagga tcaagtcttg gaacgccagc caaacttgaa
     6001 gaaggaaggc ttggtaggag gtggagtgta tcttgacttc cgtaacaacg atgcgcgtct
     6061 cgtgattgaa aacatcaaac gtgccaacca agacggtgcc ctcattgcca accacgtgaa
     6121 ggcagaaggc ttcctctttg acgaaagtgg caagattaca ggtgttgtag ctcgtgatct
     6181 cttgacagac caagtgtttg aaatcaaggc ccgtctggtt attaatacaa caggtccttg
     6241 gagtgataaa gtacgtaatt tgtctaataa gggaacgcaa ttctcacaaa tgcgcccaac
     6301 taagggagtt cacttggtag tagattcaag caaaatcaag gtttcacagc cagtttactt
     6361 cgacacaggt ttgggtgacg gtcgtatggt ctttgttctc ccacgtgaaa acaagactta
     6421 ctttggtaca actgatacag actacacagg tgatttggag catccaaaag taactcaaga
     6481 agatgtagat tatctacttg gcattgtcaa caaccgcttc ccagaatcca acatcaccat
     6541 tgatgatatc gaaagcagct gggcaggtct tcgtccattg attgcaggga acagtgcctc
     6601 tgactataat ggtggaaata acggtaccat cagtgatgaa agctttgaca acttgattgc
     6661 gactgttgaa tcttatctct ccaaagaaaa aacacgtgaa gatgttgagt ctgctgtcag
     6721 caagcttgaa agtagcacat ctgagaaaca tttggatcca tctgcagttt ctcgtgggtc
     6781 tagcttggac cgtgatgaca atggtctctt gactcttgct ggtggtaaaa tcacagacta
     6841 ccgtaagatg gctgaaggag ctatggagcg cgtggttgac atcctcaaag cagaatttga
     6901 ccgtagcttt aaattgatca attctaaaac ttaccctgtt tcaggtggag aattgaaccc
     6961 agcaaatgtg gattcagaaa tcgaagcctt tgcgcaactt ggagtatcac gtggtttgga
     7021 tagcaaggaa gctcactatc tggcaaatct ttacggttca aatgcaccga aagtctttgc
     7081 acttgctcac agcttggaac aagcgccagg actcagcttg gcagatactt tgtcccttca
     7141 ctatgcaatg cgcaatgagt tgactcttag cccagttgac ttccttcttc gtcgtaccaa
     7201 tcacatgctc tttatgcgtg atagcttgga tagtatcgtt gagccaattt tggatgaaat
     7261 gggacgattc tatgactgga cagaagaaga aaaagcaact taccgtgctg atgtcgaagc
     7321 agctctcgct aacaacgatt tagcagaatt aaaaaattaa gaaaaaataa aagaggtgga
     7381 gggcagcatt ccttgtcgcc cgtcccttct ttttaatgga gacagaaaga tgatgaatga
     7441 attatttgga gaatttctag ggactttaat cctgattctt ctaggaaatg gtgttgttgc
     7501 aggtgtggtt cttcctaaaa ccaagagcaa tagctcaggt tggattgtga ttactatggg
     7561 ttgggggatt gcagttgcgg ttgcagtctt tgtatctggc aagctcagtc cagcttattt
     7621 aaacccagct gtgaccatcg gtgtggcctt aaaaggtggt ttgccttggg cttccgtttt
     7681 gccttatatc ttagcccagt tcgcaggggc catgctgggt cagattttgg tttggttgca
     7741 attcaaacct cactatgagg cagaagaaaa tgcaggcaat atcctggcaa ccttcagtac
     7801 tggaccagcc atcaaggata ctgtatcaaa cttgattagc gaaatccttg gaacttttgt
     7861 tttggtgttg acaatctttg ctttgggtct ttacgacttt caggcaggta tcggaacctt
     7921 tgcagtggga actttgattg tcggtatcgg tctatcacta ggtgggacaa caggttatgc
     7981 cttgaaccca gctcgtgacc ttggacctcg tatcatgcac agcatcttgc caattccaaa
     8041 caagggagac ggagactggt cttacgcttg gattcctgtt gtaggccctg ttatcggagc
     8101 agccttggca gtgcttgtat tctcactttt ctagtttata ctcttcgaaa atcaaattca
     8161 aaccacgtca gcgtcgcctt accgtactca agtacagctt gcggctagct tcctagtttg
     8221 ctctttgatt ttcattgagt attagaaaac aattatgttg atagagcttg ggcaagagcc
     8281 caatttcagc aaaaaatgaa gtaaatcttc tcataataaa acgcatcata tcaagcacga
     8341 aaattccacg aggtcaacta cagtcagaaa gctgaacaac aagccaaaac gcccaaaaaa
     8401 ggcggcaaaa agcaagcacc tgcaagcaac gtgccgaaat ggtcaaatcc tgattatgtc
     8461 aacgaattag acccaaaaat cgttgatatg ctagtagaat ttcacaagtc acaaggcact
     8521 ttggaaactc ccgaggcgca agcagaaatc gcccaaaaac gtgaagaaat cgagcaaagg
     8581 agagctgagc ttgagggtaa aaaacaagag cttttgaacc gcttgaacaa atagagtttc
     8641 gcaagtatta tgcttacaaa ttacttgagc aattaactaa aatataaacc ctgcctttat
     8701 atctaggcag ggtttatatt ttagaaattc acgtaggttg ttacggtttt tacataccca
     8761 gtatagtttg agtttctata gtattcagtg ataaacttcc attttctttg agcaacatgg
     8821 atataagtac ttgttatgta gtatggatat gggctttgtg aatccaagta agactgataa
     8881 gcttgtatac caaaatatgc tccaccaatt attgcacccc atggaccccc caataaagca
     8941 cctatcctac caatcatata actgattcca gcaccagtca tgaagttagc gaatgtgtta
     9001 gcttgtttat tcccatgtat tgtgttgacg taattccaaa cattaggatc gtatgatcta
     9061 aaagatatat ttaggtcgat ttcattcttt tgataagcca tataaaatgc cccattgata
     9121 tagacgccgt cagcacgtcg ttcaatagtg tctacacttc catctggatt gacaacctca
     9181 agaacttcat cgcttaaaat atttacttgc gtatctccga accgcactga tgagccattc
     9241 tcaaactgag cctcaccaga tacaacttta gagtttgccg ataagctatc atcagcaaaa
     9301 acaaacaagc gacggggaaa tgctagacat acagaaaaca gacataacta gcaaacacat
     9361 gcatttaaac atcttagaca taacggaaac tcctttgtat ttttgatttt tttcaacttt
     9421 tattatacaa taaaaccaaa taaaaagaaa gcggtaacaa tatgcttaat gcgaaaattt
     9481 tttatatatt tttatgtttg atcgttatcg aaactacagg cttgttgttg ttgaaaagag
     9541 gtctcgaaat gggttattta gacacagaag ctattatcct cgcagttttt tcatttgctt
     9601 tttacaacct atgttcattc gcttgggtct gctctacaat aaaaaacaat aaaaaataaa
     9661 tagacgtatt ttcaaaaaaa aaaaaaaatg catatttata ttagcaaaac gacgatttaa
     9721 atcgtcgttt ttttgt
//
LOCUS       DBDESG010000071         9639 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.071, whole genome shotgun sequence.
ACCESSION   DBDESG010000071 DBDESG010000000
VERSION     DBDESG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.071"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1409)
                     /locus_tag="ACKB38_001956"
     CDS             complement(<1..1409)
                     /locus_tag="ACKB38_001956"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGL7980664.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLT"
     gene            complement(1642..2574)
                     /locus_tag="ACKB38_001957"
     CDS             complement(1642..2574)
                     /locus_tag="ACKB38_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGL7980665.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2571..3428)
                     /locus_tag="ACKB38_001958"
     CDS             complement(2571..3428)
                     /locus_tag="ACKB38_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGL7980666.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3432..4778)
                     /locus_tag="ACKB38_001959"
     CDS             complement(3432..4778)
                     /locus_tag="ACKB38_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGL7980667.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(4791..5075)
                     /locus_tag="ACKB38_001960"
     CDS             complement(4791..5075)
                     /locus_tag="ACKB38_001960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7980668.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5079..7109)
                     /locus_tag="ACKB38_001961"
     CDS             complement(5079..7109)
                     /locus_tag="ACKB38_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGL7980669.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7301..8308
                     /locus_tag="ACKB38_001962"
     CDS             7301..8308
                     /locus_tag="ACKB38_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGL7980670.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8311..8511
                     /locus_tag="ACKB38_001963"
     CDS             8311..8511
                     /locus_tag="ACKB38_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGL7980671.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8663..8845
                     /gene="rpmF"
                     /locus_tag="ACKB38_001964"
     CDS             8663..8845
                     /gene="rpmF"
                     /locus_tag="ACKB38_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGL7980672.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            8861..9010
                     /gene="rpmG"
                     /locus_tag="ACKB38_001965"
     CDS             8861..9010
                     /gene="rpmG"
                     /locus_tag="ACKB38_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGL7980673.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9502..>9639)
                     /locus_tag="ACKB38_001966"
     CDS             complement(9502..>9639)
                     /locus_tag="ACKB38_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGL7980674.1"
                     /translation="ESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         3148 a         2046 c         1528 g         2917 t
ORIGIN      
        1 tccgtcaaga gggccacctt atctccctga cctttaccaa caatcattga agaaagtgat
       61 agcatctcag gcataccagg accaccttta ggtccaacaa aacgaacaac gactacatcg
      121 ccatcaacga tttcatctgt cagaacggcc tgaatcgcat cttcttctga gtcaaagacc
      181 ttagctggcc caacgtgacg acgcacttta acacctgata ccttggcaac tgcaccgtca
      241 ggagcaaggt tcccgttcaa gatgataagc ggaccatccg cacgttttgg attttcaagt
      301 ggcatgataa ctttttggcc tggagtcaag tctgcaaagt cagccaagtt ttcagctaca
      361 gtcttaccag tacatgtgat gcgatctccg tgaaggaaac catttgccaa caaatacttc
      421 ataaccgcag ggacaccacc gacttcgtag aggtcttgga agacatactg accagatggt
      481 ttcaagtcgg ccaagtgagg cacacgttct tgaatcgtat tgaagtcctc aagtgacaag
      541 tcaacatttg cggcatgggc aatggcgagc aagtgaagag tggcgtttgt agaaccaccg
      601 agagccatcg ttacagtgat agcatcttca aaggcttcac gagtcaagat atctgatggt
      661 ttgagaccaa gttccaacat cttaacaaca gcacgtcctg ctgcttcgat atcttctttc
      721 ttatcagctg attcagctgg gtgagaggat gaccctggca aactcatccc tagaacttcg
      781 atagcagttg ccatggtatt agcagtatac ataccaccac aaccaccagg gccagggcag
      841 gcattacatt caagacgttt cacgtcctca gctgtcatgt caccgtggtt ccattttccg
      901 ataccttcaa agacagaaac caagtcgata tctttaccat caagatttcc cggtgcaata
      961 gttccaccat aggcgaaaat agctgggata tccatattag caatagcaat catagatcca
     1021 ggcatgttct tgtcacagcc accgatagcg acgaaggcat ccacgttgtg accactcata
     1081 gccgcctcga tggagtccgc gatgatgtca cgagatgtta gagagaaacg cataccaggc
     1141 gttcccatag cgatcccgtc cgctacggta atggttccaa actgtacagg ccaagcgcct
     1201 gcagatttga caccttcttt agccagtttc ccgaaatcat gcaagtgaat gttacatggt
     1261 gtattttccg cccaagtcga aatcactccc acaatcgatg tttcaaagtc cttatctgtc
     1321 ataccagtcg cacgaagcat agcacggtta ggtgatttaa ccatgctgtc ataaatgcta
     1381 ctgcggtgac gtttatctaa ttcagtcatc ttatccctcc catttcagtt tttactatta
     1441 tagcacaatt ttcgcatgaa gaacagaata aaattcttga attttcagaa aattctatac
     1501 acatgtgaaa tatttaaaat taaaaacaac aaagcggatt agtgcacttt ctgatgacca
     1561 gaatatgctt tttaatccgc tttctttaaa taacgtactg taatttttac agaaattctt
     1621 tcaaataagt gtatttaaca tctatcttgc attataaatt tctagaacct tctcttttat
     1681 attcgattca ctcaaaccat actcattaag aagataatcc attttcccta cttgaccgaa
     1741 tctttcttga acacccatcc gatgaatttt tgttattcca tcatcagaga ataattcaca
     1801 taaagcactg ccaattccac ctatctgatt gtggttttct acagtaaata tagtttttcc
     1861 acttaacatt gtttttatct gttctggtat cggtttgatt ctaaataaat ctatcacacc
     1921 tactgaataa cctaatttag acagttcatc tgcaactcga atacttggag caaccattat
     1981 gccagaagca acgattacaa gatcttcacc atgccttaac tcaatgtagc ctttagaaaa
     2041 atcttctcca ccttgataca caggaactgg agcttttcta attgttcgaa tatattttag
     2101 tccttttaag tctaatgtct ggttcaatat ttcacgaaat tggatatcat cagttgcttc
     2161 gaaaatgatt gatttaggaa ttaaacgtaa caatccaatt tcttcaaatg gcatatgtgt
     2221 tccaccattc atctctgccg ttactcctgc atctgatcca atcacagtgg catccaattg
     2281 tgcgtatcca agagaaataa ataattgatc aaatactctt cgtgaagcaa aaggaccaaa
     2341 tgtatgaaga taaggtctaa acccctgaat agacaagcct gctgcaaggc cgaccatttc
     2401 tgcttccata atcccaacat tcacataacg gtctccaaag tccttttcaa gattattagt
     2461 agccatcgaa cttgacaaat cggcttctaa gactactata tcagaatcac tttgattagc
     2521 ctctaaaagg aagtctctat atacatgccg taattctttc gtacttctca tcattctgtt
     2581 tcctccaatt cctgacttaa tctttctaca actgaagtta acatttgttt ctcctctaca
     2641 gtagggcgaa gatgatgatt ggatttcatt tcttccagct cttgaacccc ttgaccttta
     2701 atagtatcta atacaatgca cttaggtgat gaattatttg actgttttaa ttggacaatc
     2761 ccttcataaa tttctctaat atctgaaccc ttgaccctaa tggattcaaa tccaaatgct
     2821 gaaaattttt ctacgaaatc acctggatta caaatatcct ttgtaaaacc atctaattgt
     2881 tttttgttat catcaacaaa tacaattaag ttggataact gttgatgaga agcaaactgt
     2941 atagcctccc aacattgtcc ctcatttaac tcaccatctc caacaatagc gtaagtataa
     3001 aagggactct ttcttattct ctgaccatat gcaagtccag ttgcaacact aattccttgt
     3061 cctaaagagc ccgttgtcat atctatgcct ggcgttagat ttctatcagg atgagacggt
     3121 aatttggttc catttgtatt taaagaatat aagaattctt tgtcaaagaa accattcaaa
     3181 tagagtgtac tgtatagagc tggtcctccg tgaccttttg ataatatgaa ataatctcta
     3241 tctcgtgctg caaatatttc tggagtcatt ggcattattt caccataaag caccgctaaa
     3301 acttctacga tagacagact tcctccgtaa tgtccgaatc caagatgatt caatgttcta
     3361 agagtattta atcggatgtt agtcgcaaat tttcttaacc catcttctct atttttactt
     3421 aaaatcatcc cttattcctc cgttgcagat ggctttttaa taaaggatac tccaaacata
     3481 actgctagaa taagaacaag accaatcaca atgcctgctt gtgagccaaa ttgatttaac
     3541 attcctaaaa taattcctga tagaccaaaa tctgcatctg agaaagttga tccttggaaa
     3601 ccaagtcctc ccaaaactgg cattaaaaag actggaagaa aactgattaa aataccttgt
     3661 aaaaatgctc caatagtggc tccacgaaca ccaccagatg cattcccaat gacacctgca
     3721 gtcgctccac agaagaaatg aggcacaaca cctggtaaga taacaaccgt tcctgaagca
     3781 atcataatta ccatacttac taaaccacca acaaaactag agataaatcc aattagaact
     3841 gcattgggtg cataagtata aacaatcgga caatccaaag caggttttga attaggtaca
     3901 agacgctctg aaataccttt aaaggctgga acaatttcgc ccaaaataag gcgaacacct
     3961 gctaaaataa caaatacccc tgctgcaaat tgacctgcta attgtaaagc ataaactaga
     4021 ccacttgtac cactactgat ttctttttct atatattctg accctgcaaa gatagctaca
     4081 ataatgtaaa taactgccat ggataaagta atactaacag tactatcacg taaaaaagct
     4141 aaactctttg gaaatttaat gtcctctgtt gattttgatt tgtcaccgat aaggctacca
     4201 gtaaaaccac tcaaccaata tcccaaagaa ctgaaatgac ctaaagctac cttgtcattt
     4261 ccagttaatt gaaccatata tttttgcaca aatgctgggg aaatactcat aataataccg
     4321 agtgctaatc ctcctagtaa gatgagaggc aagctagtaa agccagcaac tgataaaatg
     4381 accgcaatca tacatgccat atatagagtg tggtgccctg ttaaaaaaat atatttaaat
     4441 cgagtaaaac gagcgattaa gatattgaac accatgcctg caaacataat cattgcagta
     4501 gctgagccat atgttgttaa agctacagct acaattgctt cattattcgg cacaacgcca
     4561 gataaatgaa aagcatgctc aaacatggta ccaaatggat ttaaagaatt ttgtacaatt
     4621 cctgcaccac cagatacaac taagaaacca acaaaggtct taattccacc tttaataata
     4681 tcaggtaatt tcttcttctg aagaactaat cctaagattg caattaaagc tactaaaata
     4741 gctggtgtac taacaatatc caatatgaac ttcatcatga cgctagcctc ctatataagt
     4801 cctttttctt cacaaagttt agtaattaat tctcgtagtt catccatatc aataatacta
     4861 tttaagatac gaacatctcc aagatgacta gctgaatcag ctagatcacg accaacaatc
     4921 caaatatcag ctgcatttgg atctgctcca cctaaatcat aatgttcaac ttctacatcc
     4981 gaaacattca aatcactcaa tacagattca atattcatct gtaccataaa acttgaacct
     5041 aatcctgaac cacaagctgt accaattttt aacattatct aatcctcctg tttaattatc
     5101 attttaatgt catcatagtt ttttgatgat attaaagttt gaacatgatt tttatctctt
     5161 aaaattgttg ttaaatgtga caaagccttt aaatgactct cattatcaat ggctgcaata
     5221 caaatcaaca atcttacctc ttgttctgga ttatccaata aataaatcgg ttcttccaaa
     5281 actaacattg acattcctat ttcattcaca ccttcatctg gccgagcgtg aggaattgct
     5341 actcccttcc ctaaattaat aaaaggtcca aactcttcta ctttttgaat cattgcctca
     5401 gggtagttct cagttatctt atcttgatcc aaaagcggtt tagctgctaa acgaatcgcc
     5461 tccttccatc ctaatttttg cgaactaacc tgataggttt ctttggtaat aagttgttct
     5521 agcactggta caatttcctt tctatcattt ttttggtaaa gataattctt taacgccaat
     5581 cttaattcca attcttgtgt aataattcca tatcttttga caatattcag gatttgttca
     5641 atctcaaaat ctccatactc taaattcgga aaatctttta acactagttc tactagttgt
     5701 attgcttgct cttcagtcat cataaccgaa actagataat ttggcttttc tgtctccacc
     5761 tttatggtag aaaaaaccat atcatagtca ctactagctt tcacctgtaa atcatcaatc
     5821 tttgaggttc ctataaactc aatttgagga aataatgcta atagattctc ttttaacatc
     5881 aatgaagaac taacaccatt aggacaaatg attgctgctt tataccattt ttgaggcaaa
     5941 gtatctgctt tctttaaata acctccgaaa tggataacaa aatatgctgt ttcactatca
     6001 ggtatgggat tgtcaatagc gtccatcaag ggcatcaaag aatctttgac taattcaaat
     6061 aaatcaggat aatgttcttt aacatgcaat acatattcat ttgaactagg taggccgaac
     6121 tttaatctat agtaagccgg tataaggtgg cggcgaagat tttctctcaa tccttccctt
     6181 tgtttaaaat gtaacaaaga aatatcttcc attctactta taatagcctc tgttaattga
     6241 ttaaagtaaa ccggagcaac atctacttca ccttcaaagc aacttgataa taaaacggtg
     6301 atatagcgat aatcatcctc agaaaacacc gtatctataa ttcccaaatc aaccactgta
     6361 tccaataaaa tagtggttat atcttgaata acaggagata ctaatgtctc tgaaagacat
     6421 actctttcaa catccctttg atacctacac agaatgaata ctaaaccgaa aaggtaaact
     6481 tttaattgat taacaatagg tactagctgt agcttctcat aataatcttt aactacctga
     6541 tcaatcaaat cataagttaa tgaatacccc caactggata aaacataatc caaaccccaa
     6601 atccctatgg aggattccag caactcacta accatttgaa aagctaagcg gtgcttattc
     6661 cactctgaac cgtgtaaagt ataacctttt gctctactgt accctagctc caaatcatta
     6721 tctaacataa tctttcttaa tgattgaata tcagataagg ttgtattctt acttactttc
     6781 aaaaagtctt ggtaatgact attcgatata aaatctaatc ggcaaaaagt gtaaagatag
     6841 attaaagcta agcgagtcga ctttggtaaa accaattcat ccgacttaat aatatctgtc
     6901 aaagactgct tcgtacgatt tgataaacta tagcgacctt gctttttatc cagcactatc
     6961 cctttattag ctagataagg cactaaataa tctattcctt ctttgacttc ctttataggt
     7021 aagctcacct taacagataa ttcatataat gatagctcac aatgatccat caaagtcatc
     7081 aaaataacta gtgctctata atcaaacatt tctcttcctt tctaactaca attatagtgt
     7141 aaaaattata agaataaaag agtatttgag cacaatataa tctcaccttt taaaagaaat
     7201 tattgggctg aaagttttta gtgattattt ttcaactttc tattgacttt ataaattaaa
     7261 gtgatatcat ttagatatca aataacagga ggtcattctt atgactgaaa aactaatcaa
     7321 ttcaaaacca aatggtgtat tcgcattgat tctcattgag ttgacaatcg tacttggtat
     7381 ctttatattt ataatggggg ttggttcgga aaacattttt ggaattatta tcggaccttt
     7441 actaatcgta attgcaggtc tagctcatgc tggtttaaaa gttgtcaaac ctcaagaagc
     7501 tctggttctg acactctttg gtaactatac aggtaccatc aaagaacctg gcttttactt
     7561 tgtcaatccc ttcagcgtag cagtcaaccc tgcaaaccac actcgacttg gacaaagtgg
     7621 tgatgttagc acaaaatctc cttttttagg agctaaatca tcaaatgaca atgatgtaaa
     7681 tcttgaaatt ggcaagaaac agatttccct caaagtcatg accttgagca attctcgtca
     7741 aaaaatcaat gattgcttag gaaaccctgt agaaatcggt atcgcggtaa cttggagagt
     7801 tgtcgatacc gctaaggcag tcttcaacgt tgataactac aaagaatatc tttcattgca
     7861 atgtgatagc gccctccgta atattgtccg catctatcct tacgatgtgt ctcctaatgt
     7921 ggatactacg ggtgatgggc aagcagatga aggtagtctc cgtggctcta gcgaaattgt
     7981 tgctaaccgt attcgtgaag aaatccaaag tcgtgttgag gatgctggct tggaaatcct
     8041 tgaagcacgt atcacttacc tagcttatgc tccagaaatt gctgccgtta tgcttcaacg
     8101 ccaacaagca tctgccatta ttgatgcacg gaagatgatt gtagatggtg ctgtaggaat
     8161 ggttgaaatg gcactagaac ggctcaatga aggggaattg gtagaacttg acgaagaacg
     8221 aaaagctgcc atggtttcaa atctccttgt cgttctttgt ggcaatcatg atgcacaacc
     8281 aattgtcaac acaggaagcc tttactaaag atggctgaag ctaaaaaaaa gcagatcccc
     8341 cttcgactct caacaaagtt atacgctgca ctcgcatcat gggcagaaga cgactttcgt
     8401 tcagtcaatg ggcaaatcga atatctcctc acagagtgtg tcaagcaacg gaagaaagac
     8461 ggaaaatacg tatcagaaac cattgacgaa ccatttgaga ttgatattta acaccttctc
     8521 ctgtctgccg tgtcggatag gagaattttt tcatcttttt ttgtcaagta actttacttt
     8581 acaaaaaaaa tgtgttatcc tagtatggtt gatgaaaatc agtagattga atcgaatata
     8641 aatacctaaa ggagaaatca aaatggcagt acctgcacgt cgcacttcaa aagcgaagaa
     8701 aaacaaacgt cgtacacact acaaagtaac agctccatct gtaaactttg acgaaactac
     8761 tggagattac tcacgttctc accgtgtatc acttaaagga tactacaaag gacgtaaaat
     8821 cactaaagct gcatcagctg aataatagaa gggagatacc atgcgcgtaa atattacact
     8881 tgaacacaaa gaatctggtg aacgcttgta ccttacttct aaaaacaaac gtaacactcc
     8941 agaccgcctt caattgaaga aatactcacc aaaacttcgc aaacacgttg tgtttacaga
     9001 ggttaaataa gcattcaata cgaatcaata cagaagaaaa acgcttgatc agcgtttttt
     9061 ctttttgtta atttcactac attgtaaggg aaatcattcg aatgactaca tttttcacta
     9121 ccttttttga aataagaata cggattcgga caattaaaag caagaggaac tcaaatgtaa
     9181 gttggggttc cttttttgta tatttttcaa ataacgcctc tacactattt gtagcaaatt
     9241 caccaactac agttgtatct tagttaaaat aagttagaat atgtaagtga gtaccagata
     9301 taccaagaca tcgtcaccat ctaaggtata ttcaaaatac aaaagttgac caactagatt
     9361 tctgaatatc cttatatatc cattcttaaa attggtttaa atagcgtagt cttttaaact
     9421 agttttgaga atccaaaaaa tcttcctaca tatgtaagaa gattttttag ttcagaatga
     9481 ttagatttag ctaatggata cctatcctac ccactcaccg ttagcattga ctcgatagcc
     9541 atcgggtgta gtcgtgttta ctaataaatc tcctgaatta taggtatagt accatttacc
     9601 agaaactgta acccaaccag tagccattga acctgattc
//
LOCUS       DBDESG010000072         9504 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.072, whole genome shotgun sequence.
ACCESSION   DBDESG010000072 DBDESG010000000
VERSION     DBDESG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.072"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..965)
                     /gene="mnmE"
                     /locus_tag="ACKB38_001967"
     CDS             complement(<1..965)
                     /gene="mnmE"
                     /locus_tag="ACKB38_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGL7980675.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDR"
     gene            complement(1102..1284)
                     /locus_tag="ACKB38_001968"
     CDS             complement(1102..1284)
                     /locus_tag="ACKB38_001968"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGL7980676.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            1408..2007
                     /locus_tag="ACKB38_001969"
     CDS             1408..2007
                     /locus_tag="ACKB38_001969"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGL7980677.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            1997..2476
                     /locus_tag="ACKB38_001970"
     CDS             1997..2476
                     /locus_tag="ACKB38_001970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980678.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            2486..3565
                     /gene="prfA"
                     /locus_tag="ACKB38_001971"
     CDS             2486..3565
                     /gene="prfA"
                     /locus_tag="ACKB38_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGL7980679.1"
                     /translation="MNIYDQLQVVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            3565..4404
                     /gene="prmC"
                     /locus_tag="ACKB38_001972"
     CDS             3565..4404
                     /gene="prmC"
                     /locus_tag="ACKB38_001972"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGL7980680.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILAENPVKNLMVLDIGTGSGAIALALAKNRPDWSVTAADISQDALDVASENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            4388..4990
                     /locus_tag="ACKB38_001973"
     CDS             4388..4990
                     /locus_tag="ACKB38_001973"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGL7980681.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     RRDKALNLNVASLEDILYFSKNQPTYLQKLVEIFLPGPLTIILEANDRVPYWVNSGLA
                     TVGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFNQILEDFDQEVLGLEDDAFL
                     TGQDSTILNLSGDKVKILRQGAIKREDILARLPKISFEEE"
     gene            4992..5423
                     /locus_tag="ACKB38_001974"
     CDS             4992..5423
                     /locus_tag="ACKB38_001974"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980682.1"
                     /translation="MLRDLRETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            5485..6741
                     /gene="glyA"
                     /locus_tag="ACKB38_001975"
     CDS             5485..6741
                     /gene="glyA"
                     /locus_tag="ACKB38_001975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGL7980683.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            6749..7723
                     /locus_tag="ACKB38_001976"
     CDS             6749..7723
                     /locus_tag="ACKB38_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGL7980684.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAKSFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            7723..8337
                     /gene="pmp23"
                     /locus_tag="ACKB38_001977"
     CDS             7723..8337
                     /gene="pmp23"
                     /locus_tag="ACKB38_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGL7980685.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            8411..9385
                     /locus_tag="ACKB38_001978"
     CDS             8411..9385
                     /locus_tag="ACKB38_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGL7980686.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
BASE COUNT         2948 a         1853 c         2032 g         2671 t
ORIGIN      
        1 tggcgatctt gggcggtcag tggttcacta gcgtttagta ctagcagaac taggtcagct
       61 tccttaagag cttttttcga acgctcaact ccaatttgtt caacgatatc atccgtttca
      121 cgaataccgg ctgtatcaat caatttgaga ggtacaccat tgatgttgac gtactcttcg
      181 atgacatctc gtgttgtccc agcgatatct gttacgatag ccttgtcctc acgcaagagg
      241 ttgttgagaa ggcttgattt cccaacgttg ggacgtccaa tgatagccgt tgaaattcct
      301 tcacgaagga ttttaccacg acgtgctgtc ctaaggagtt tggttagtaa ttgctcaaac
      361 tccattgtct tctctcggac aacagcagta gtggcttcct caacatcgtc atactcagga
      421 tagtcgatat tgacctcaac ttgggcaagt gtattgagga tttcttgacg ggtattgtta
      481 atgaggtcag aaagggagcc gtctaattgt ttgaccgcaa tgttcatggc cttgtcagtc
      541 ttggcacgga tgatatccat cacagcctct gcctgtgtca agtctacgcg accgtttaaa
      601 aaagcacgtt tggtaaattc accaggttct gccaaccgag ccccttcacg aatagctagc
      661 tggagaattt cattggtcac cgcaatccca ccgtgggtgt taatctcgat aatatcctca
      721 cgagtgaagg tctttggaga cttcatagcc ccaaccataa cctcgtccat gactttacca
      781 gtcagaggat caataatgtg accgtagttg agagtgtggc tggcaacctt gttcaagtct
      841 tttcctttaa aaatcttttg cgcaatagca aaactgtctg ttccgctcag gcggacaata
      901 ccaatagccc cttcacctag tggagtagag atagcagcga tggtatcaaa ttcacgtgta
      961 atcataatgt ttcctttaat taatagctct tttcttggaa attctccaag tttcttttca
     1021 aattgtaaca aatttaggat atttcttcaa tgaatgctac ttggagattg aaaaagccta
     1081 agcaattctg cttaagctag cttatttagt acgcatttcc ccttgtggga agtaagttcc
     1141 ttctggcatg tcgttgatga tgacatggac agcagattga ggggctccag tgttgcggac
     1201 aactgcttcc gttacttcct tagcaagagc tttcttttgc tcgagcgtgc gtccttcaaa
     1261 taaatcgatg cgtacaaatg gcataatagc ttcctccact agtttttgat ttcttctatt
     1321 ttaccacatt ttgccgttta aagcttaaga aaattatgat atactagaat gtagcaaaaa
     1381 tttagaaatg gacgtgaagc aagaaacatg gcacagttgt actatcgtta tgggaccatg
     1441 aactctggta aaacgattga gattctcaaa gtggcctata actacgagga gcaaggaaaa
     1501 ggtgttgtga ttatgacctc ggctctggat acgcgtgacg gcgttggcta tgtgtcgagt
     1561 cgaattggca tgaaacgccc tgcccttgcg attgaggaaa caacggatat ctttggctat
     1621 atccgagacc taccagaaaa accttactgt gttttggtcg atgaagccca gtttctcaaa
     1681 cgccaccatg tttacgacct agctcgtgtt gtggacgagt tagacatacc cgtcatggct
     1741 tttggcttga aaaatgactt tcgcaatgaa ttgttcgaag gttccaaata tctcttgctt
     1801 ttagcagaca agattgacga aatcaagacc atctgtcagt attgcaagaa aaaggcgacc
     1861 atggtgttgc gaacacagga tggactgccc gtttacgatg gagaacagat ccagattggt
     1921 ggtaacgaaa cctatatctc agtttgccgt aaacattatt ttgcgccaat gataacctct
     1981 aacaaggagc aaaactatga caattgaact aagagatgtt acaatggaaa attattttga
     2041 tgttttaaat ttggatgtca aggaatatca aaaacaattc attgcaacca acgcaattag
     2101 tttagctgaa gcatacgtct acactaaaaa tggagatttt gtagctccat tggcagttta
     2161 tgataatgat gcaattatag gttttgtgat gatagcttat gataaaaaga tcggaattag
     2221 tagtggaaat tatttactat ttcgttttat gattgataag aattttcaaa atcaaggata
     2281 ttttaaacca attatggata aagtactaga ctatgttcga acagcgccag caggtttaag
     2341 caataaactt tggttgtctt atgaaccaga aaatgaacag gcaagatttt gttacctcag
     2401 ttatggattt aaagaaactg gggaaatatc cgagaatgaa gtagtagcaa tctatgattt
     2461 aacaattgag aaataaggag aaaaaatgaa catctatgat caactacaag ttgtagaaga
     2521 ccgttatgaa gaattaggag aattgctgag tgaccctgat gtcgtttcag acaccaagcg
     2581 ttttatggag ctttcaaaag aagaagcttc caatcgtgac accgtaatag cctaccgtga
     2641 gtataaacaa gtccttcaaa atatcgtcga tgccgaagag atgattaagg aatcaggcgg
     2701 agatgcggac ttggaagaat tggccaagca agaactcaaa gatgccaagg ctgaaaaaga
     2761 agaatatgaa gaaaaactga aaattttgct ccttccaaag gatccaaacg atgacaagaa
     2821 tatcatcctt gaaatccgtg gagcagctgg tggagacgaa gcggcacttt tcgctggaga
     2881 tttgctaact atgtaccaaa agtatgcgga agcccaaggt tggcgctttg aagtcatgga
     2941 agcctctatg aatggtgtcg gtggttttaa agaagtggtt gctatggttt caggtcagtc
     3001 tgtatactct aagcttaagt atgaatcagg tgcccaccgt gtgcaacgtg ttcctgtgac
     3061 agaaagccaa ggccgtgttc atacttcgac agcgacagtt cttgttatgc cagaagttga
     3121 agaggttgaa tacgacattg atccaaaaga ccttcgtgtc gacatctatc acgcctctgg
     3181 tgctggtgga cagaacgtca ataaggttgc gactgccgtt cgtatcgttc acttgccaac
     3241 caatatcaag gttgagatgc aggaagaacg tacccagcag aagaaccgtg agaaggccat
     3301 gaaaatcatc cgtgcccgtg ttgctgacca ctttgctcag attgctcaag atgagcagga
     3361 cgctgagcgt aagtcgacaa tcggtactgg tgaccgttca gaacggatcc gaacttataa
     3421 cttcccacaa aaccgtgtca cagaccaccg tatcggcttg accctccaaa aactagatac
     3481 gattttgtct ggtaaattgg acgaagttgt ggatgccttg gtgctttatg accaaacaca
     3541 aaaactagaa gaattaaaca aataatgaaa ttagctcaat tattttcaaa ttttgaagaa
     3601 gagttgataa gacaaggaga ggaagcggaa agcctctctt ttgtctatcg tagtctgaaa
     3661 aatctatctt ttacagactt tatttttgcc ctccagcagg aggtgacaac agaggaagaa
     3721 aaacaatttg tagaagatat ttaccagcaa ctagcagctc acaagccagc tcagtacatc
     3781 attggacagg cagattttta tggaatgcat ttaaaggtgg atgaacgggt tttgattcct
     3841 cgtccagaaa cagaggagtt ggtggagctt atcctggctg aaaatcctgt gaaaaatctt
     3901 atggtcctag atatcggaac aggtagtgga gctattgctc tcgctctagc aaaaaacaga
     3961 ccagattggt cagtgacggc agcagatatt tcccaagatg ctttagatgt agctagcgaa
     4021 aatgctaaaa atcaaaatct tcaaatattt ttaaaaaaat ctgactgttt tacagaaatt
     4081 tctgaaaaat atgatataat tgtttccaat ccaccctata tctctcgtga agatgagtca
     4141 gaggtaggct tgaatgtctt gtattcggag cctcatctag ctctctttgc agatgaggat
     4201 ggcctagcta tttaccgtag aattgcggaa gatgcaaaag actatctcaa agatggtggt
     4261 aagatttacc ttgaaattgg atacaagcaa ggtcaaagtg ttcctgaact ttttaggaaa
     4321 catcttcctg aaaagcgagt acgaacactc aaggaccaat ttggtcaaga taggatggtt
     4381 gtcgttgatg atggacagga ttagacaaga gttggaaaat ggtggagctg tcgttttgcc
     4441 tacagagaca gtttacggtc tctttgctaa ggcgctagac gaaaaagcag ttgaccatgt
     4501 ttaccagctt aaacgtcgac gtagagacaa ggcgctcaat ctcaatgttg cctctttaga
     4561 ggacatcttg tacttttcaa agaatcagcc aacttatctt caaaaacttg tagagatctt
     4621 tttaccaggt cccttgacca ttattctcga agctaatgac cgagttccct attgggtcaa
     4681 ttctggcctt gcaactgttg gatttcggat gcctagtcat cccattaccc ttgatttgat
     4741 tcgagagaca ggtcccttga ttgggccgtc tgccaatatc tcaggtcagg cgagtggagt
     4801 gacctttaat caaattctag aggattttga ccaagaggtt ctgggtctgg aagacgatgc
     4861 ttttctaact ggacaggatt caactatttt gaatttgtcg ggagacaagg tgaaaatctt
     4921 acgtcaaggg gccattaagc gagaagatat tcttgctcgg ttgccaaaga tttcttttga
     4981 ggaggaatga gatgctaaga gatttaagag aaactgatgt gaaagctata tgtgacatca
     5041 accaagaggc tttgggttat acttttagtc cagaggaaac ggctagccaa ctagctagac
     5101 tgtctcagga ttcccatcat ttcctacttg gctatgagga tgcagctaat catgtcttac
     5161 ttggatatgt ccacgctgaa gtttacgaat cactctattc caaagcagga tttaatatct
     5221 tagctttagc agtttcacct caagcgcaag gtcaaggtat tggtaaaagt ttactacaag
     5281 ggttggaaga agaagcaaaa agatgtggtt atgggtttat ccgcttaaat tctgccaatc
     5341 atcgtctggg tgctcatgca ttttatgaaa aagttggcta tacttgtgat aaaatgcaga
     5401 aacggtttat tcgcatctgt tagtttgatt ttcttattgt aaaatcaaac taatggacta
     5461 gtcacacaat aaaggagaag acctatgatt tttgacaaag atgattttaa agcatatgat
     5521 gctgatctct ggaatgctat tgccaaagaa gaagaacgcc aacaaaataa tatcgagtta
     5581 attgcttcgg aaaacgtagt ttccaaggct gttatggcag ctcaagggtc tatcttgaca
     5641 aataaatatg ccgagggtta cccaggacgc cgttattatg gtggaactga tgtagtagac
     5701 gttgtagaga ctcttgctat tgaacgcgca aaagaaattt tcggtgctaa atttgccaat
     5761 gttcaaccac attcaggaag ccaagctaac tgtgcggctt acatgtcctt gattgagcca
     5821 ggtgatacgg ttatgggaat ggatttggca tcaggtggtc atttgactca tggggctcct
     5881 gttagcttct ctggtcaaac ctacaacttt gtttcttata gtgttgatcc taaaacggaa
     5941 ctcttagact ttgatgctat cttgaaacaa gcccaagaag taaaaccaaa actgattgta
     6001 gctggtgctt cagcctattc tcaaattatc gatttttcaa aattccgtga aatcgcagat
     6061 gctgtcggtg cgaagctcat ggtggacatg gcccatatcg ctggcttggt tgcggctggc
     6121 cttcatccaa gcccagttcc atacgctcat atcacaacaa caacgaccca caaaaccctt
     6181 cgtggacctc gtggtggttt gattttgacc aatgacgaag aacttgctaa aaaaatcaat
     6241 tcagctattt tcccaggtat tcagggcggt cctttagagc atgttgtggc ggctaaggca
     6301 gtttccttca aagaagtttt ggatccagcc ttcaaggaat atgctgccaa tgtaattaag
     6361 aacagcaagg ctatggcaga tgtcttcttg caagaccctg atttccgtat tatttcaggt
     6421 ggaactgaaa accatctctt ccttgttgat gtgactaaag ttgtagaaaa cggcaaagtt
     6481 gctcaaaact tgttggatga agtcaatatt accttaaata aaaactcaat cccttacgaa
     6541 agcttgtcac cattcaagac aagtgggatt cgtatcggag cagcagccat tactgcacgt
     6601 ggatttggtg aagaagaaag tcgcaaagtg gctgaactca tcattaaaac ccttaagaat
     6661 tcagaaaatg aggctgtatt agaagaagtg agaagtgcag tcaaagaatt gacagatgcc
     6721 ttcccattat acgaggacta aaacttttat ggacatttat attaagaaag ccattattca
     6781 ccagttcagt ccggatgata ccgaactgtt cttagcagat aagtttctca atattactcc
     6841 aaaaatcgaa gaatacctgc gtaaaaaaat tgaacatgtg tattcagatg aagccaagac
     6901 tgggattttc gaagaagaaa atcccttctt caatcatatt acagacgatt tgttggagac
     6961 atcagtaacg ctggctaatc tctggaaaga ggagtttagc atttctgaaa atctcaagac
     7021 caatgacttg atttttgtgc aattttctaa agaaggtgta gaacatttcg ctttcttgcg
     7081 aattgccctg cgggagacct tgacccacct cggaggagaa gttgataatc caatcaagct
     7141 aactcagaat aacctgcctg gatttggaac aggagctgat gaggccttgg tggtcaatct
     7201 tcagagtagc aagtatcacc tgattgaaaa acgaatcaag tacaacggga cttttttgaa
     7261 ctatttttca gataatcttc ttgctgtcgc tcctaagatt tctcctaaaa aatctatcaa
     7321 ggaactggaa aaaacagccc agagaattgc taaatctttt aacacagatg attttcaatt
     7381 tcaatccaag gtcaaatcag ctattttcaa caacctagaa gaaagcaatg aattgtcacc
     7441 tgagaaattg gctaatgacc tttttgacaa caatctgacg gctcgtttga gctttattga
     7501 ccaagtcaaa gaagccgtac cagaacctgt tcaatttgat gaaattgatg ccagtcgcca
     7561 attaaagaaa tttgaaaacc aaaaactctc cttatcaaat ggaattgagc tcatcgttcc
     7621 caataacgtc tatcaagacg ccgagtctgt tgagtttatc caaaacgaaa atggaaccta
     7681 ctctatctta atcaaaaata tcgaggatat ccaaagtaaa taatgtttaa acgaattcga
     7741 agagtgcttg tactagcagt cttccttttt gctggctata aagcttaccg cgttcatcaa
     7801 gatgtcaaac aagtcatgac ctatcaaccc atggtgcgag aaatattgag tgaacaagac
     7861 accccagcaa acgaagagct tgtgcttgct atgatttata ctgaaacaaa aggaaaagaa
     7921 ggcgatgtta tgcagtctag tgagtctgca agtggttcca ccaacaccat caatgataat
     7981 gcctctagca ttcggcaagg cattcaaact ctgacaggca atctctatct ggcgcagaag
     8041 aagggggtag atatctggac agctgttcaa gcctataatt ttggacctgc ctatatcgat
     8101 tttatcgccc aaaatggcaa ggaaaatacc ctggctctag ccaaacagta ctctcgtgag
     8161 actgttgccc ccttgcttgg taataggact ggaaagactt atagttatat tcaccccatt
     8221 tccatttttc acggtgctga actctatgta aatggaggaa actattatta ttctagacag
     8281 gtacgactta acctttacat catcaaatgt ttcactctct tttcaacatc tggctagacc
     8341 aggtgttttt gttataagtt ttctttaaga tagatatatt actctaaaga agtaaaggag
     8401 gaaattccct atgagaaaga aactctttct gactagcgct gcgatcttgt gggcagtaac
     8461 agctatgaat agcgtccatg cagcaacaga tgttcaaaaa gttatcgatg aaacctatgt
     8521 ccaacctgaa tatgtcctag gttcctccct atctgaagac caaaaaaatc aaactcttaa
     8581 aaaactgggc tacaatgcct caacagatac caaagaactc aagaccatga cacctgatgt
     8641 ttattctaaa atcatgaatg tggccaatga ctctagctta cagttgtatt catcagccaa
     8701 gattcaaaag ctaggtgaca aatcgccact tgaggtcaag attgaaacac cagaaaatat
     8761 cactaaggtg actcaggata tgtaccgaaa cgcagcagta acgctgggta tggaacatgc
     8821 caaaatcact gtagcagccc ctattccagt tacaggagaa agtgctttag cagggattta
     8881 ctattcgcta gaggctaatg gagccaaggt gccacaagct aataaagatt tggctcaaga
     8941 agagctaaaa gctttgtcag atatcaatgc tgaaaacaag gacaaatcag gctatgatgc
     9001 taataaatta aacgttgccc tagctgatat caagtcagga ctcgccaaag ctaaagaaag
     9061 taagggaaat ctgacagaag aagatatccg caagattgtt gaagatacct taaaaaatta
     9121 caaacttgat caggtcataa caggaaacca gatcaatatc atcatcaatt ttgccttgaa
     9181 tctctcaaag agtgatatcc tcagcaatgc agatttcact aaaaccctaa atgaccttaa
     9241 acaaagcatc gtatcacaag ctggcgacag ttttaaaaat atcaacctta actttgatgc
     9301 ggataaggcg ctagaggacg gtggtaactt cttaagctcc ctctggcaag cccttgtcaa
     9361 cttcttcaag agttttggtt cttaagaaat ttcatggtat aatggatggt aaccgtaacg
     9421 ttgccgtctt ttttgtcggc caatagaaag agaaaagaat gttaaagaaa aatgatattg
     9481 tagaagttga aattgttgat ttga
//
LOCUS       DBDESG010000073         8803 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.073, whole genome shotgun sequence.
ACCESSION   DBDESG010000073 DBDESG010000000
VERSION     DBDESG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.073"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..379)
                     /gene="srtC-1"
                     /locus_tag="ACKB38_001979"
     CDS             complement(<1..379)
                     /gene="srtC-1"
                     /locus_tag="ACKB38_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HGL7980687.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVL"
     gene            complement(717..1898)
                     /gene="rrgC"
                     /locus_tag="ACKB38_001980"
     CDS             complement(717..1898)
                     /gene="rrgC"
                     /locus_tag="ACKB38_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HGL7980688.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDDRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKTDTMTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDVSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIFVTNLPLGNY
                     RFKEVEPLAGYAVTTLDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEESGHYTPVLQNGKEVVVTSGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            complement(1945..3942)
                     /gene="rrgB"
                     /locus_tag="ACKB38_001981"
     CDS             complement(1945..3942)
                     /gene="rrgB"
                     /locus_tag="ACKB38_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HGL7980689.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAAIMGIAVYAYVKNNKDEDQLA"
     gene            complement(4166..6826)
                     /gene="rrgA"
                     /locus_tag="ACKB38_001982"
     CDS             complement(4166..6826)
                     /gene="rrgA"
                     /locus_tag="ACKB38_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HGL7980690.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDSENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            7343..>8803
                     /gene="rlrA"
                     /locus_tag="ACKB38_001983"
     CDS             7343..>8803
                     /gene="rlrA"
                     /locus_tag="ACKB38_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HGL7980691.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIV"
BASE COUNT         2535 a         1916 c         1377 g         2975 t
ORIGIN      
        1 gcaatacctc ttcagcagta ccagcataaa ccggcaaatc cacgtcaata acggggattt
       61 ccacatgccc catccgctca tggatttcta acatacgtgc atactctgct cgcccttttt
      121 tcttcatttc ttccgaccaa ggatcgccac tcactacatt attcaaagag tcattgaagg
      181 cttgtgccaa tttcattcgt tcatcaatgt cagcctcatc caacgttgct ttttccttat
      241 caaagtcagc aatttgttga tttgattcca ctcgataata caagcgagac accagcggat
      301 acgccattac cgccattcca atgaaaaata ccactcctaa taggagatta tttcgttttt
      361 gcttttttgt ttttaccatt tttatcagca tccctttatc ttcaaactca tcattttatc
      421 gcttttccta acagacaatc ctatctgcta agagaaacga gaaaatctca tttctttctg
      481 attcaccatc cattttaaat cagaatccca gtcctgtcca ttcctaaaat gattaccaga
      541 ccagtcctat cgttttacaa acaataaccc acaatgaggc tggcacaatc tgcccagcct
      601 catatccgtt attgtgattc atgccacatc accatgattc ctgattaaca tcacctcaga
      661 gtactctggt cttcccttca gcctgctatc tttttcataa tgatgtacat tgaatatcag
      721 ttatttggtt ttttcgtaag ataataacca ctaccaaaca acaaaatggc aacaagcatc
      781 aagatataca aggtttcttc ccctgtatct ggcacatcaa tccgtggtcg cttgttattt
      841 ttaaccactg ttaccagttc cttacgagta tctttcccga ttgtaaagga aacaggcgat
      901 gttaattgaa cataaccagt tggagcttgg agctcccata aatagtatgt cccatactct
      961 agaccttcca ctcggaaacg accatctttc cctgatgtta caactacttc cttaccattt
     1021 tgaagaacag gagtatagtg tccgctttct tctttcatga ctttgaacat tgccccttga
     1081 agagaggtat tggtccgacc atccaccttc ataaagtcaa cattgccacg tggtaatttc
     1141 tgattgacaa ccgtaatcgt caccagctga tgatctacca gctggacatc cgtatccagc
     1201 gtcgtaacag catagcctgc cagtggctcc acctccttga aacgatagtt cccaagagga
     1261 agatttgtca caaaaatctc tccattttta tcagtataga gagttctccc tacttgacca
     1321 gaagaactgt aacggtattc tccaatcaag ggaacctctt tttcagaaac atctcttgct
     1381 actgatacca atttaaagcc gacaccctcc aagcgattgt ggtcttgatc cacctttatc
     1441 agcttcacct ttgttgtcat tgtatctgtt tttttcgcta caatgaccaa aggctctacc
     1501 gtttgatctg tcatttcaaa aagaaattca gctggataag aaaccgcatc cgtctggata
     1561 atagagcgaa catagtaaag accatttgga atatgagata cttcaatctg attctcaagg
     1621 aaggtcatct caaacgaagt ctttttgaaa gaagaaagtt tattctcatc ccacgaatgc
     1681 aagtctctta caatttgcac ccgatcatca taggaatacg aatcatccaa cttccatact
     1741 tgcaaccgat gaccatcacg agatggcaat tgactaacca cctcctgata gttctccaat
     1801 tgcaagacca acgtgtgatc ttcttgcgct tggactgcat gtgcacccca tacaagagaa
     1861 aaacacagag ccataacaaa gaagatacga ctaatcattt tctgcatttt ctgcattgtc
     1921 atcaatggct cctttctctc ttacttaagc aagttgatcc tcatctttgt tgtttttaac
     1981 atatgcgtac actgcaatac ccataatcgc agcccccgct acagcaaaga taattgtacc
     2041 aataccaccc gtttgtggga tagtgatttt tttgttgact acttttgtag cgtcatcttt
     2101 acctgaacca gcagtatact caatgccttg tccagtcgct gaataagaag ttgcagtgac
     2161 ttcaaatttc tgacggctag ttagtaatgc ataaccagca ggctgttttg tttcttctaa
     2221 gtaatatgta cctgcaagaa ggcctgtaat ttcaaagcga ccttgtgcat cagaaactaa
     2281 tttcacaaca ttttcattgt ccttatctgc cacccattca aatgcattgt tggcagcaat
     2341 cacagcagca ttataagcag cttgagcttt gtcaactttc tctttttctt gctgagtttg
     2401 ttgttgtgca gtaagagcgt tataagcagc aactgctcta tctaaagcat cctttgttgt
     2461 aacaaccaac tgcttctctt cttgactcac tttatctgct ttacgtgcta aatattgacc
     2521 agcattatca gcatttgcaa ttacaaattc tgccccagct aaacgattat ctttatcatt
     2581 aactttgaca aactttttac catatgtaac aacttttggc tctgttggat caagtggttt
     2641 tggattttcg tctttccagt tcttgacagc aatttctcca gctgtagtga tttcttgata
     2701 atctgctgaa taccctttta tactacgttc aacgaattta tattctgtat ttttatccaa
     2761 tccgttaaca gtaactgtat ttttgtctgt tgtcaaagtt acagtttgta caactttacc
     2821 agtctgagca ttaaccaaat cgaacgttgc ttcagctcca gccggaattg gtgcacctgt
     2881 agcatcaacc catgtcttgg tcaatgtcaa atcgccgttt tcatttggct tattcggctt
     2941 tggagtattc ccgtgatctg gattattacc atagttaaat gttacatcat tagattctgg
     3001 tacttctaca attgctttgt cattcaatgt tgccgaataa gtgattttca cagttttttc
     3061 agcgttttgg tcattcactt tagctaaacc agcatctgtt aatttcaaat caaaaccagt
     3121 tgctacttct gttagagcat aatcacctgc ttcaagtgca acatcatcaa cagttacttt
     3181 cactgtacct ttgttgaatg ccaaaccttc agtcattcta tcgctccagt ttgctgttgc
     3241 ataattagca agtgctggaa tttttgtaac aatttcgtac tctacaacat ctccaacttg
     3301 gtggttcaca ggtgtatctt tatctacacg tggtgtatct ggatttgctt tacctttgaa
     3361 atctttatca atttttggct ttgcttctgt attttttgga tacacatgcg catccacaac
     3421 atcgttcaat ggtaattcaa tttcaattgg aactgcttta gaacctgtta aggttgctcc
     3481 atcttcaccg acataagttg ataaactgtg aatttcataa attttatact tagcagctgg
     3541 taaatttgcc gtgttaaatt tagctccttc agcttctgtt aattttttca ttgcagttgc
     3601 cggcattgcc cctgagagtt taaatgtttg tggatcaata ttcactccta gagtttggcc
     3661 attttcatca ataatttcat tattagtatt tgtccaaacg aacataacac cggcaatttc
     3721 ttttgcattt gcaggtagaa cacccacttt attaccagca tagttacctg tttctaactc
     3781 atttgcaatt ttatccatat ccccatctgt tgccaatagt ttatgaacgg taacagatgt
     3841 tgttgtcgtc ccagccgcaa aaactgttgc agctgaaaac aggctactcg ctgtcagtaa
     3901 taaggcagca agcattgtta aaaatttgtt gattgatttc atgatttctc cttattcata
     3961 tctcttaaaa tcaactgtga gcaatgtaat gaattacaca tgacaataat aatccttttt
     4021 ctgtgcaagt tttcgaaaac atctcccctt tcaatgatag tttacttacg aataaaacag
     4081 gtttcaccaa gtaaacttaa atctcttctt gagtgctact tcttaaaagt atcgttcaga
     4141 acatagatat tatcatttct acactttacg gatgtttccg tgtgtataat agaactcctc
     4201 ccatcatcat gcaacctatc agatagaatg gcaacattcc gataccacca gttcgaggat
     4261 attctctctt tggaggaata ggttcattgg taatatagtg cttatcattc gtaaactcgt
     4321 aacccgctgg tatatcttgt ggaacgattg aagtcacatc tctgacttct ccatttacta
     4381 tttggaaggc aacgataggc ttattttgaa cgggtttata accagctggt tcagaatttt
     4441 caaataatcg atatttccca tctgacagat ttttaaaggt caacttacca tcttcacctg
     4501 ttctcacatt ttgataagtg ccattttgat caatagctcc ataaatatct ggataatccg
     4561 gatgttgttt ttgaagacta aagaccgcac ctctcagtgg ttttttatca tttttattga
     4621 ccttaataaa ctcaatgtca ccaagttttt tctcttttga aattgtgatt tctggatact
     4681 tccgcacatc acgaatctta ggaatcggga agtcgcgcac tgtgttctgt tctacttcct
     4741 taggatgtaa ggttgttcga ccattggtat cataaaattt attgcttaca aactcatcat
     4801 tcaaacgaac attgtaggtc aacgtaactt tttcatccgt tccaaggtac agacctgtta
     4861 cacgaatcct tttctcagtc gtatcataga gcacttttgc attttttaac aaaccaccat
     4921 cattttgtgg accacctaca gcttgtccat tctccaagcg actaccatcg tttgcagtta
     4981 aagtgtaatc tgctggatca aatcttccat ctgtgcccaa ttgcaaatca attaactcac
     5041 ccatcggatc tgtaatcgta ccattctcaa ttgatttctt ttcagttacg atggtgtgga
     5101 aataacgatt caactgttcc aatatttttg tcgtgtcagt aacattggta tagttttcag
     5161 gtttactaga aatactttgc ataaaactag tagccgttgc ttcatccgta ccaggatctc
     5221 cgttaatacc aatacctacc gtaaagacat catacccatc aggagcaata ttcccgttat
     5281 agtaccatct tgtagggtca ccatgattgg taattttagc agtattagaa ttaaacggat
     5341 aagccagaat actctctctc caattaagcc aaatatatcc accattaatt ggatcgccta
     5401 taactgcata accagccgcc ttcatttcag aatatttttc aggtttaact gggaaagctg
     5461 caggagcacc tttttcataa actgtcttat ctgtaaacat ctggtaactt tgcccatctc
     5521 cgcgtacaat tgtatgttct ccactagttg cttgcgtaat aaaatcactt aatagtattc
     5581 catctttatt aggagattta ctaaaaaatg catttagttg attttgatat gatggagcaa
     5641 acgtagcatg attaaaatta atcggatacg acatagttgg gacaccatcc gtaatatgga
     5701 aaatgacttt ttgactattt tgtctcgctt gttgtgtcaa aatctcatct gccttcatca
     5761 aagctttctg agtaaaagtg gcaccgaatt ggtacatcaa tctatttcca tcatggtctt
     5821 ctgcctcggt aggtacctta ttttttaatt ctacaatgtc attcttatca ttagtcagct
     5881 ttaaataact ataatctttg gtattggttg taaaactcgt ctgatcataa ttccaaaaaa
     5941 gagaatcatt caatcgcttt ccgtttttat ctgctacccc tttttctact gtaaactcgg
     6001 tcccatcaaa gatagtggaa gcataagtca caagcgctac cctattttct gaatcagatg
     6061 taattttatc aataagagaa cgtgtcgcct caccagctct ttccgcacgt cgagcattct
     6121 tgtttcgaat gttactcata ctatttgagt tatcgagcaa gataacgaca tccagcggca
     6181 cagacttatc tttttgttca tacactgttt tcccactaac cgtcaattcg attccatatt
     6241 ggttatcatc caaattattc acttgataaa ttctctttga aagtgtacct tctggaatca
     6301 cacgttcata tggattcgga ttcaacgcct tgtgctgtcc gtttttttcc gaaccatcta
     6361 ccttaataat ctgataaggt gtttgaacat ctggataagt ccctgtttgt ggatactggt
     6421 cagatagagc ctcttctcga ttttctacct gttcaccttg gacagtcgtc cgaccattct
     6481 tctcaacttc aacagtccat tgtttagtag agggtttata accaactgga ggttgggctt
     6541 ctgtcaaggt gtatgtccca ggttttatgt ttgaaaatat cgcttctcct gtttgcgcct
     6601 ctgtcctttg cgaaacagtt gtgccatccg tattgttttt caactcaaag acggcatctc
     6661 ctagaagcgc tcctccttcg cctgtctcct taatcactac ttttcctatc gctggactgg
     6721 tttcaggcgt ttctgctaaa gcaactatcg aagaaaaagc tgtcaactga gatatacagc
     6781 acagtcctgc aactgccttc tgaaatatct ttcttacttt tttcatgtgt gtctccctgt
     6841 taagcattat tcatagaaca agttaatcct tctatgattc ataggtactt tcaatctgta
     6901 tacacgaaga aacggattac ttatgttctg ataaaattta tctcactgag ttaatccgtt
     6961 cactaacaaa cgtagcaaaa gcgtataaaa gtgcgttata gctatcttct caacctggca
     7021 atgacacact tgtatacaat atagtatagt atagtattac tattaactat cctagtataa
     7081 attaaaacaa cttccatcac aatagatatt tttatcacat atttttttta tagaacgaca
     7141 atatttgata aaaaaatatt ttctaataaa aaaaataagc cataaaactc caccaatact
     7201 cattactatc tttaaaatgc cttttattac atgctgtttt atcaataaag tcggaaattc
     7261 cacaaccttt ttgacaagac aattagtttc ttttgtatta taataattaa aattatctat
     7321 atgcaactaa gattcggtaa ctatgctaaa caaatacatt gaaaaaagaa ttacagataa
     7381 aattaccatt ttaaatatat tattggatat tcggtctatt gaactagatg aactgtctac
     7441 tttaacttca ttacaatcta aaagtctcct aagcattttg caggaattac aagaaacatt
     7501 cgaggaagag ttgacattta atttagatac gcaacaagtt caattaatcg aacaccattc
     7561 tcatcaaacg aattattact tccatcagct ttataaccaa tcaactattt taaaaatact
     7621 ccgcttcttt ctactacaag gtaatcaaag ctttaacgaa tttacacaaa aagagtatat
     7681 ttccatcgca acaggctacc gcgtacgaca aaagtgtggc ttgttactac gttcagtagg
     7741 tttggacctt gtgaaaaatc aagtagttgg tcctgaatat cgcattcgtt ttttaattgc
     7801 actcctccag tttcattttg gtattgagat ttatgatttg aatgatggaa gtatggattg
     7861 ggtcacacat atgattgtgc agtctaattc tcaactcagt catgaactgt tagaaatcac
     7921 acctgacgag tatgttcatt ttagcatact agtggcactc acttggaaac gtcgggaatt
     7981 tccacttgag ttccctgagt ccaaagagtt tgaaaaatta aaaaacctct ttatgtatcc
     8041 gattttgatg gaacattgcc aaacttactt agagccacat gccaacatga cttttactca
     8101 ggaagaactg gattatatct ttttggttta ttgttctgct aattcttcgt tttccaagga
     8161 caaatggaat caagaaaaaa agactcatac tattcaactg attcttcagc atacaagagg
     8221 aaagcatcta ctatccaagt tcaagaatat cttaggaaat gacatttcga attctctttc
     8281 ttttttaaca gcactgactt tcttaacaag aacgtttttg tttggtttac aaaatctagt
     8341 tccctattat aattattacg aacattatgg gattgaaagc gacaagccac tgtatcatat
     8401 ttcgaaagca attgttcaag agtggatgac tgagcagaag attgaaggag ttatcgacca
     8461 gcatcggctg taccttttct cactctactt gacagaaacc atcttttcct cactacctgc
     8521 tatcccgatt tttatcattt taaataatca agcagatgta aacttaataa agtccattat
     8581 tttacggaat tttactgaca aagttgcctc tgttacagga tacaatattc taattagtcc
     8641 tcctccttca gaggagcatt tgacagagcc attgattatt attacaacga aagaatacct
     8701 gccttacgtt aaaaagcagt atcccaaagg gaaacatcac tttttgacga ttgccttaga
     8761 tttgcatgtt agccaacagc gtctcatcta tcagacaatt gta
//
LOCUS       DBDESG010000074         8593 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.074, whole genome shotgun sequence.
ACCESSION   DBDESG010000074 DBDESG010000000
VERSION     DBDESG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.074"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(164..796)
                     /locus_tag="ACKB38_001984"
     CDS             complement(164..796)
                     /locus_tag="ACKB38_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGL7980692.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(818..1690)
                     /gene="sdaAA"
                     /locus_tag="ACKB38_001985"
     CDS             complement(818..1690)
                     /gene="sdaAA"
                     /locus_tag="ACKB38_001985"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGL7980693.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1699..2370)
                     /gene="sdaAB"
                     /locus_tag="ACKB38_001986"
     CDS             complement(1699..2370)
                     /gene="sdaAB"
                     /locus_tag="ACKB38_001986"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGL7980694.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2612..3199
                     /locus_tag="ACKB38_001987"
     CDS             2612..3199
                     /locus_tag="ACKB38_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGL7980695.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3251..3577)
                     /locus_tag="ACKB38_001988"
                     /pseudo
     CDS             complement(3251..3577)
                     /locus_tag="ACKB38_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4002..4289
                     /locus_tag="ACKB38_001989"
     CDS             4002..4289
                     /locus_tag="ACKB38_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGL7980696.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            4341..6449
                     /locus_tag="ACKB38_001990"
     CDS             4341..6449
                     /locus_tag="ACKB38_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGL7980697.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            6446..7087
                     /locus_tag="ACKB38_001991"
     CDS             6446..7087
                     /locus_tag="ACKB38_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980698.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            7294..8100
                     /locus_tag="ACKB38_001992"
     CDS             7294..8100
                     /locus_tag="ACKB38_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGL7980699.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATKLGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            8117..8509
                     /locus_tag="ACKB38_001993"
                     /pseudo
     CDS             8117..8509
                     /locus_tag="ACKB38_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
BASE COUNT         2598 a         1529 c         1878 g         2588 t
ORIGIN      
        1 atccacaacc ttaaaacagt gttttgagct gacttcgtca gttccatcta caaccttaaa
       61 acagtgtttt gagctgcccg cagctagttt cctagtttgc tctttgattt tcattgagta
      121 ttacttcatt ttcttctgaa atggaattgt tacccagtct atgctattga aaatacgcca
      181 aaacttctaa gggtttgtga gcgatataat caggttgata gtttagtaga tctgcttgct
      241 ctccaaatcc ccaagtgatg gccaatttct gaatacctgt ttctcgagct cccagcatat
      301 caaacttggt atctccgatg atgatggctt gttctggtgc tagttgatgt gtctgcaagg
      361 cttggtgaat gacatctgcc ttatggggtg cttcagggct agaaccataa atgccatcaa
      421 agaaatgatg gatttccaag ttttttgcca tgtcttgagc agtagatgta tcctttgtcg
      481 tggtgatgta gagtggataa ctgctcgata actcctcaag caagtctata atctgaggaa
      541 agagttgagc ttcatagatg ccttttgcct tatagtaaga acgatatatc tgcacggctt
      601 cagaaatttg gtctttggac aggcaggtcg caaaactact ttcgagaggt ggtcccataa
      661 aaccacgaat agttttggca tcagggctag gcacccccag ctctttaaag gtataggtaa
      721 aggcattgtg aatcccgata gaactatcaa cgagggttcc atccaaatcg aaaaaaatcg
      781 ctgtgataga ggtcatggtt tctcctattt gataagctta ttctccgaaa atttcttttt
      841 ggaggcgacg accagtaggg gtggtagcga gtccaccttc agctgtttca cgaaaggcag
      901 ttggcatgct tgctcctact tggtacatgg catcgatcac ttcatccaca gggattttag
      961 attcgatacc tgccaaggcc atgtctgctg cgatgaaagc aaagctagct cccatggcat
     1021 tacgtttgac acagggaact tcgaccaaac ctgcaacagg gtcacagatg aggcctagca
     1081 tatttttaat gacaaaggca atagcttgac tggcctgata aggtgttcca cctgcagcca
     1141 gagtcaaggc ggcagcactc atagcagagg ctgaaccaac ttcagcttga cacccaccct
     1201 cagcacctga gatggaggca ttgtttgcga tgactagtcc aaaggcacca gcagcaaaga
     1261 ggaaatccaa ttgttgctcg tggctgaggt ctaatttttc aatagcagca gtgagaacgg
     1321 atggcagaca gccagcactt ccagcggttg gagtggcaca gaccaagccc attttggcat
     1381 tgtgttcatt gactgcgatg gcatttcggg cagccgagag aatcgtataa tctgacagag
     1441 tttttccgtt ttcgatgtag tgatccaatt tggcagcatc tccacctgtc aggccactac
     1501 gagatttatt ttcattgagg ccaagttgga cagaggcttt cataacttcc agattgcgtt
     1561 ccatgagaag gaagacttct tcacgttcgc gaccggtcaa ttcaaactct gttgtaatca
     1621 tgagttctgc gacatttcct tgaaagtcca gatctgcttg ctcgaccaat tctttgatag
     1681 aataaaacat gcttcctcct atttaaagaa attgacattg tggagatgag ggatttttcg
     1741 aatttcttcg atagcctcat cacagttgcg actgtcaact tcgataatca taatggcttt
     1801 ttcaccagct ttttcacgag tgacattcat ctgggcgata ttgataccat agcgggaaag
     1861 cgcctctgta acaagggcaa tcatacctgg aatatcttga tgaacgatga tgatagtcgg
     1921 tgtattcata ttgagagaga cggcaaaacc attgagttcg gttacctgaa tatttcctcc
     1981 accgatagaa ataccagtca cgctgatggt cttgtgggca tttttaacag taattttagt
     2041 ggtgttaggg tgaggggcat tgctgtcttt ctgaatggtc cagacaatct tgataccacg
     2101 cttgtgggca atttccagac tatttggaat ttcaggatca tctgtatcca ttcctaaaat
     2161 acctgcaaca agggctaggt ctgttccgtg accacgatag gtcttggcaa atgagttaaa
     2221 aagttggaat tcaacttctg tcggagtatc atcaaaaatg gaagagacaa tcttcccaat
     2281 acgaacagca ccagcggtat ggctactaga tgggccaatc ataactggtc cgatgatatc
     2341 aaagacagat tgaaaacgaa gtgatttcat cagtttcccc ttataaaaat tcttatctct
     2401 attatatcaa agaatgaggg gcttggcttt aattgtggat gaaaaccttt ctaatacctc
     2461 aaatagcata aaaatagtat cttttatgac aaaaaacacc ttatttaggg aaataaaaaa
     2521 taattttgta atatttctac ataaaagtgt caagaaacgg taatatttaa agggtatgat
     2581 agaactatag aaagaaggag aattttcgaa tatgaaatca ataactaaaa agattaaagc
     2641 aactcttgca ggagtagctg ccttgtttgc agtatttgct ccatcatttg tatctgctca
     2701 agaatcatca acttacactg ttaaagaagg tgatacactt tcagaaatcg ctgaaactca
     2761 caacacaaca gttgaaaaat tggcagaaaa caaccacatt gataacattc atttgattta
     2821 tgttgatcaa gagttggtta tcgatggccc tgtagcgcct gttgcaacac cagcgccagc
     2881 tacttatgcg gcaccagccg ctcaagatga aactgtttca gctccagtag cagaaactcc
     2941 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     3001 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3061 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3121 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3181 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3241 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3301 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3361 aatcgtctgt acaaatccca atagggacat agcagatagc cagcctatcg taacttttct
     3421 tattcttggc gtcctccagt aaagtatagc agatttgctt acacatgttt ttatcttgat
     3481 tgtatagata gagtgtggaa aggttgagca ggattgctat tcgcaagtca tataaacgtt
     3541 gatagttttt atagacttct aagcgttgca agattttctc ggattattga caaataaacg
     3601 gacattctga tagacgattg ccctagcggc ttagtgtaaa ctttacaaat ttggcttgta
     3661 atttttttat aaaattcact tcagatactt gacagttatt atattttcgc ttatgcttat
     3721 tatatagata aaaggacggg ataggagttt aaaaatttga aagcgctatc catattgttt
     3781 attggactga ttcttgagta atagttagag tttgcttaat actactctgg ttgttcaagg
     3841 acctgataca gaagcgcacc attctcccgg ttaagagtaa gttcgttgtt aaaaaatgaa
     3901 atgagataga aggaaggtga gtccaatgga ctacgatact tgttgataaa ttactatagc
     3961 tgtttttacg gaacagctct cgataatagg agaaataaat catgagaaaa ttagttaaaa
     4021 ttggtgttgc atctttaatg gtatgtggta tacttgctac tacaaatgct ttagcagtat
     4081 gggttgaggg tggtcaatgg aactatggag taggttggac aggaactttt ggatattctg
     4141 attacttaca ttctactcga tatcatacag caactgttag acatgggggt agaacctcta
     4201 aggattatgc aaaacctgag gcatgggcta gagcttccct caccaagatt ccgccaacag
     4261 gaatggaata tttctatgga tttgagtaaa attcttccct ctcctatttg gagagggaaa
     4321 actgtagtgg aggcactttt atgaaaaaaa tcagtaattt ctgtatgtta ctcctgcttc
     4381 tgtgtaccac tttttttgtt tttaatgtaa actatacacg agaagtggtt cggattcaag
     4441 aaatgggaaa gactgtagat tctttggatt tgtatttgaa agatattaac gaacctgcag
     4501 cgtctgttct tcgatttttt gaggatgtat caaaggagta taaagtctcc atcatcaaaa
     4561 cagacagtgg tgatgaggtg gtcaagtctg gtgtttttga taaagatacc ttcccctacc
     4621 aagagtttgg gatttcttct cttgatttta ccacagatgg tgaaggagtc tatagtaata
     4681 aagaaatttc caataaactt ggtacgattc cgacctttct aaaagccaaa cctattcagc
     4741 ttatgacttt tcaaacctat atcaaggata catctcgtag tttaaatggt cgctatacga
     4801 taacttctac acaagagatg gacaaggata ggattgtaca gaaatggagc gattttttca
     4861 agatagacca ggctaccttg ctagagccga cctacaaaag tgcagtggaa gtcataaatc
     4921 gagatttgct tttatctgcc attgtttttg tcttggctat tttgcttctt gtgttagtga
     4981 cagtgtatca accgatgatg gagatgaaaa gagttggggt acaaaaatta cttggttttc
     5041 aagatagggc tgttttagct gatgttgtaa aaggcaacct ttacctcctc ctaggtgggg
     5101 ctcttgtgat caatctaggc gtgtttttct tgcttgatta taagccaaaa gatttgtttc
     5161 ctatgctgtg gttgtctcat tttttgctgt tgcagcttta tctctttatc agttggttga
     5221 cttacctctt aatccaaaaa atgacaatca gctctctgct gaaaggtttt tcatctttca
     5281 aatttggtct tatcttcaat tatgtgatga aaatagggac aactatttta ctgacggcct
     5341 tactgattgg ggtgggcaga agtttagaac aagaaaacaa agaacttgct tatcagcaac
     5401 agtgggtaag tcaaggtaat tacctgacct tagaaacctt caaactcaat gataatctgt
     5461 ggcaagaaga gctagcaggg tcagggaaat ctacagatta tttctatcga ttttatcagg
     5521 atttggtaga aaaaacgcag gcgggctatg tgcagagtag cagtcttcct gtaaaaaatt
     5581 ttgtccaatc agaacagatt cagcaatatc agttaacaga tacggtggat gtttactatg
     5641 ccaatcgcaa ttttctaaag agcaagggat tcaagctacc aaataccggt attaaaaaag
     5701 ttattttgat gccagcaagt acgaaaggtg aagaagataa aaatcagctc ttggggaagt
     5761 taattgcctt tcattcgatg aagtatgaag agcagcaaaa acgaacgata gaggagatgg
     5821 atgtcgagat tgcctattat gaaggagatt ggtcattttt cccatatagt gataagcgaa
     5881 aggaaaatct ctccaatcca attattagct tggtcaatga ttctgatatg atgtgggatg
     5941 agaaagcctc cctgtcaaca actggcttaa ataatccgat taaaattgaa aatacggttc
     6001 aacatcaaaa agagattaca gagttagttg agaaattgtc agatggaaat tatttaaaat
     6061 tttcatctat tcaagccatt caacaagaga aagtggattc ttatcgagat gctgttcgga
     6121 attttaacct actctttgct ttgtttggtc tccttagcat gatgatttcc tacttcttac
     6181 tagtaacaac tttcttattg aagcgcaggg atatcattac caagaagttt atggggtgga
     6241 aactggtcga tcgctaccgt cctctcctcg ttctgctctt gctgggctat agtttccctc
     6301 ttctagtctt gattttcttt gcccatgcgt tcttaccact tctactgttt gcaggtttta
     6361 catgtctgga tatactattt gtgctaggct tagcttctag gatggagaaa agaagtctag
     6421 tagagttatt gaaagggggc atcttatgat tgagttgaaa aatattacca aaaccattgg
     6481 gggaaaagtg attttggata acttatctct caggattgat cagggggatt tggtagctat
     6541 tgttggtaag agtggtagtg ggaagtcgac cttgttaaat ttattgggtt tgatagatgg
     6601 tgattatagc ggacggtatg agatttttgg tcagacaaat ctagcggtta attctgctaa
     6661 gtcgcaaaca ataatccgtg aacatatctc ttatctgttt caaaattttg ccctgattga
     6721 tgatgaaacg gtcgagtaca atctcatgct ggcgctgaaa tatgtgaaat tgcctaagaa
     6781 agacaagctc aaaaaggtgg aagagatttt agagagagta ggtttatcag ctactttgca
     6841 tcaaagggtc tccgagttgt ctgggggcga acaacaacga attgcagttg ctagagccat
     6901 cttaaaaccc agccagctga ttttagccga tgaaccgaca ggttctctgg atcctgaaaa
     6961 tagagatttg gtcttgaagt ttctcttaga gatgaatcga gaggggaaaa cagtcattat
     7021 cgtgacccac gatgcttatg tagcccaaca atgtcatcga atcattgaat taggcgaggg
     7081 aaaatgagtt cgttcagctt catttgaccg actgaatact catatttttc agagagaaat
     7141 agcataaata ggcctaggaa tgatattttt atgtcatttt cctgggtttt tctgttttaa
     7201 attgaaaaaa attacaattt aggcttgaca aaggatgcat atagatgtat tgtttataca
     7261 aataaaagga ataaacataa agtaggcttt attatgaata agatgaagaa ggtgttgatg
     7321 acgatgtttg gtttagtgat gctcccccta ctatttgctt gtagcaacaa tcaatcggct
     7381 ggaattgaag ccatcaagtc caaaggaaaa ttggttgtag ccctcaatcc ggattttgct
     7441 ccatttgaat accaaaaagt ggttgatggg aaaaatcaga ttgtgggttc agatatcgaa
     7501 ttagccaagg ctatcgcaac aaaactaggt gtcgaattgg aactatctcc catgagtttt
     7561 gataatgtac tggctagtgt tcaatcagga aaagccgacc ttgccatatc aggtgtttct
     7621 aagacagatg aacggagcaa ggtgtttgac ttttccattc cctactatac tgcaaaaaat
     7681 aaactcattg tcaaaaaatc tgacttgact acttatcagt ctgtaaacga cttggcgcag
     7741 aaaaaggttg gagcgcagaa aggttcgatt caagagacga tggcgaaaga tttgctacaa
     7801 aattcttccc tcgtatctct gcctaaaaat gggaatttaa tcacagattt aaaatcagga
     7861 caagtggatg ccgttatctt tgaagaacct gtttccaagg gatttgtgga aaataatcct
     7921 gatttagcaa tcgcagacct caattttgaa aaagagcaag atgattccta cgcggtagcc
     7981 atgaaaaaag atagcaagaa attgaaagag gcagtcgata aaaccattca aaagttgaag
     8041 gagtctgggg aattagacaa actcattgag gatgccttta aagcatccat tgaaaaatag
     8101 aaagaaggaa aagaacatga gtaaagaaaa agtaattttg gcctattctg gcggtttgga
     8161 cacatcagtt gctattacct ggttaaagaa agactatgat gttgtttcag tttgtatgga
     8221 tgtgggtgaa gggaaagact tagatttcat ccatgataag gctcttaagg tttgggcagt
     8281 agaatcttat gtcattgatg ttaaggacga atttgctaca gattatgtgc tagtggctca
     8341 tcagtcacat gcctattatg aacaaaagta tctcttggta tctgccttga gccgccctct
     8401 gatttctaaa aaattggttg aaatagcgca tcagatagga gcgactacaa ttgctcatgg
     8461 ttgtaccggt aaggggaatg accaagtcga ataccagatt gcagtcgcta agaaagcgaa
     8521 tgaagctaaa aagtgatgga gtgatgggtt cagaataaga ggttggtaga ttatcagcct
     8581 ctttcttgtc gtt
//
LOCUS       DBDESG010000075         7650 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.075, whole genome shotgun sequence.
ACCESSION   DBDESG010000075 DBDESG010000000
VERSION     DBDESG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.075"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..1574
                     /gene="rlmD"
                     /locus_tag="ACKB38_001994"
     CDS             <1..1574
                     /gene="rlmD"
                     /locus_tag="ACKB38_001994"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGL7980700.1"
                     /translation="GVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKYLVQS
                     PHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGMEH
                     PVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFFIQDPVIDQVVVALRDLLRRF
                     DLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPKVFRVDQLIEQVIKQFPEIVS
                     VMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTA
                     IDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVELIPEAVENSQKNASLNKITNA
                     HYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFIKASAQTGADRIAYISCNVAT
                     MARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMDLTS
                     AESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPE
                     KEEAIMDALRHFKMI"
     gene            1788..2066
                     /locus_tag="ACKB38_001995"
     CDS             1788..2066
                     /locus_tag="ACKB38_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980701.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            2312..2530
                     /locus_tag="ACKB38_001996"
     CDS             2312..2530
                     /locus_tag="ACKB38_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980702.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            2923..3948
                     /locus_tag="ACKB38_001997"
     CDS             2923..3948
                     /locus_tag="ACKB38_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGL7980703.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            3938..4945
                     /locus_tag="ACKB38_001998"
     CDS             3938..4945
                     /locus_tag="ACKB38_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGL7980704.1"
                     /translation="MRNKKQINLGIIFIICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            4945..5952
                     /locus_tag="ACKB38_001999"
     CDS             4945..5952
                     /locus_tag="ACKB38_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGL7980705.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            5952..6746
                     /locus_tag="ACKB38_002000"
     CDS             5952..6746
                     /locus_tag="ACKB38_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980706.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
     gene            6897..7081
                     /locus_tag="ACKB38_002001"
                     /pseudo
     CDS             6897..7081
                     /locus_tag="ACKB38_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7200..7523
                     /locus_tag="ACKB38_002002"
     CDS             7200..7523
                     /locus_tag="ACKB38_002002"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7980707.1"
                     /translation="MLGYYEDIDDKNYRVFENFISVSFLGAVFYHKYKASLDMKIHCL
                     KLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYME
                     RYRDR"
BASE COUNT         2807 a         1126 c         1368 g         2349 t
ORIGIN      
        1 caggagttgc caaggtagat ggtttggtct tttttgtaga gaatgcttta ccgagtgaaa
       61 aaattctcat gcgtgtcctc aaggtcaata aaaagattgg ctttggaaaa gttgaaaaat
      121 accttgtcca gtcaccacac cgtaatcaag atctagattt ggcttacctg cgttcaggaa
      181 tcgcggattt aggacacctt tcttatccag aacagctcaa gtttaaaacc aagcaagtca
      241 aggacagtct ctacaagatt gctggaattg cagatgtaga agttgctgaa acgcttggta
      301 tggaacatcc agtcaagtat cgcaataagg cgcaggtgcc cgttcgtcga gtgaatggtg
      361 tcttggaaac aggatttttc cgtaagaatt cgcataacct catgcccctt gaagatttct
      421 ttatccagga tcctgtcatt gaccaagtcg tagtagctct tcgagacctg ctccgtcgtt
      481 ttgatttaaa accttatgac gaaaaggaac agtctggatt gattcggaat cttgtggtgc
      541 gtcgtggtca ctattcagga caaatcatgg tcgttttggt gacaactcgt ccaaaagttt
      601 ttcgtgttga ccaattgatt gaacaagtta tcaagcagtt cccagagatt gtgtctgtca
      661 tgcaaaatat caacgaccag aataccaatg cgatttttgg taaggagtgg cgcactcttt
      721 atggtcaaga ctatattacg gaccagatgt tgggaaatga cttccaaatc gctggcccag
      781 ccttttacca agtcaatact gaaatggcgg agaaactcta tcaaacagcc attgactttg
      841 cagagttaaa aaaagatgat gtgattattg atgcctattc tggtattgga accattggtt
      901 tatcagtcgc caagcatgtc aaagaagtct acggtgttga actgattcca gaagcagtag
      961 agaatagcca gaagaatgct tctttgaaca agattactaa tgcccactat gtctgtgaca
     1021 cggctgaaaa tgccatgaag aaatggctca aggaaggtat tcaaccaacc gttatcttgg
     1081 ttgatcctcc acgcaagggc ttgacagaaa gctttatcaa agcaagcgcc caaacaggag
     1141 ccgatcgcat cgcctatatc tcctgcaatg tcgcaaccat ggcgcgtgat attaaactat
     1201 accaagagtt gggatatgaa ttgaagaaag tccagccggt ggatctattt cctcaaacgc
     1261 atcacgtcga gacggtagca cttttgtcca aactcgatgt cgataagcac ataagtgttg
     1321 aaattgagct ggatgagatg gatttgacaa gtgcggagag caaagcaaca tatgctcaaa
     1381 tcaaagaata tgtttggaat aaatttgaat taaaagtttc gacattatat attgcacaga
     1441 taaaaaagaa atgtggaata gaattacgag aacattacaa caagtctaaa aaggataaac
     1501 aaattattcc acagtgtaca cctgaaaaag aagaagccat catggatgct ttgagacact
     1561 tcaaaatgat ttaatagaaa agaatgacag tatatgactt tctgcattta ttacattcct
     1621 acttggtata ggaacagcta ttattccttt cttgcaaggt atcaattaga aaataggctc
     1681 aatataaaga ttgataggat catttttata tttaaaggag cgttgaaatg attgataaag
     1741 gcaacaaaaa attttaggat aaatttgcta agttgtatgc ctcttttatg aaaaaagata
     1801 aagaggttta tgataaagtt tgtgaatatc ttagtcctca tttgaataaa gatatggagg
     1861 tgcttgaact tgcttgttgg tttcgtgtca taacagttat agaggcaaat agttatgtaa
     1921 atataaggag ttcaagactt ctaccaaagt ttaaaactca aaaaataaat agttggtgtg
     1981 ctgcttacaa tatccatttt aataatggat attgtaagca gcacaccaat gaatttaaag
     2041 attctttaaa gagtcttatt ttgtgatgaa aatttaatat gtaaatctca gacgatagaa
     2101 attaaaaact ctatcgtctt ttttatactc aaaattagga ggtaaaaatg gtaaggataa
     2161 gaggtcccac ttaaaacaat ttatggcaaa ataaggacgg aataacacaa caaattctct
     2221 aaaacaaatc actaaatcaa tgtaagattg aatgaaatca atatttatgc tataattaaa
     2281 taaatttaat gaagaaaaaa agagggatat tatggcactt aactataaac cattatggat
     2341 acagttagca aaaaaaggac taaagaaaac agatgtaata gctatggcag gacttacaac
     2401 aaatgttatg gcacaaatgg gaaaggataa accaattaca tttaagaatt tagaaagaat
     2461 atgtaaggct ttatcttgca ctcctaatga tattattagt tttgaagata attttagtga
     2521 cgaggaatag aaaatgactt taaggacaga agatcaagtt agggattatg caagagaagt
     2581 ataggcttta atgaagttga agaaaacatc aatcaaggta ctggtcaaat aactactttt
     2641 aatcaattag gcttcaaggg atattcaaat aagccagatg gttggtattt acctaaaaat
     2701 atgaatgatg tagcaataat ccttgaaaca aaatcagaag aaagagatat tagcaaacaa
     2761 atttttattg atgagttaat gaaaaatata gacataattt aactaaaaat aaaaactaga
     2821 tccttttttg aaaaaattat attattaaat ttgtaactgt atctattgac aatgataatt
     2881 attatcgata caatagactt gaaatatgtt taaggagttt ttatgaaaaa caaatttttt
     2941 ctaatagcta ttttagctat gtgtatagtt tttagcgctt gttcttctaa ttctgttaaa
     3001 aatgaagaaa atacttctaa agagcatgcg cctgataaaa tagttttaga tcatgctttc
     3061 ggtcaaacta tattagataa aaaacctgaa agagttgcaa ctattgcttg gggaaatcat
     3121 gatgtagcat tagctttagg aatagttcct gttggatttt caaaagcaaa ttacggtgta
     3181 agtgctgata aaggagtttt accatggaca gaagaaaaaa tcaaagaact aaatggtaaa
     3241 gctaacctat ttgacgattt ggatggactt aactttgaag caatatcaaa ttctaaacca
     3301 gatgttatct tagcaggtta ttctggtata actaaagaag attatgacac tctatcaaaa
     3361 attgctcctg tagcagcata caaatctaaa ccttggcaaa ctttatggag agatatgatt
     3421 aaaattgatt caaaagcctt aggtatggaa aaagaaggtg atgagttaat caaaaatact
     3481 gaagctcgta tatccaaaga attagaaaaa catccagaaa tcaaaggaaa aatcaaagga
     3541 aaaaaagtat tatttactat gattaatgct gcagatacat caaaattctg gatttatact
     3601 agcaaagatc caagagcaaa ttatttaaca gatttaggtc tagttttccc tgaatcatta
     3661 aaagaatttg agagtgaaga tagttttgca aaggaaattt ctgcagaaga agcaaataag
     3721 ataaatgatg ctgatgtaat cataacttat ggtgatgata aaactcttga agctttacaa
     3781 aaagatcctc ttttaggtaa aataaatgca attaaaaatg gtgccgttgc tgtaattcca
     3841 gataatacac cgttagcagc ctcatgcact ccaacaccac tttcaataaa ctatactatt
     3901 gaagaatacc taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa aacaaataaa
     3961 cctaggcata atttttataa tctgcctagg tcttcttatt acaatatttt tgtcattaaa
     4021 gcttggaaca aaagaaatta atatcagaga ttttttagca gcttttggaa tgggtaatac
     4081 aaatgatgat tttattaaat caattatata taaaagaata cctagaacta tttttgcaat
     4141 tttagcaggt tctagtcttg ccataagcgg tgtattgatg caatcagtta ctagaaaccc
     4201 aatagctgat ccaggtatac tcggtataaa cacaggagca agtcttagtg tagtaattgg
     4261 tctttctttt ttaggaattt catcaagcat aagccatata agttttgcaa tcattggtgg
     4321 cttagtaagt gcaatttttg tatacgcgat tgctgtaagc ggaaaagcag gccttacccc
     4381 tataaaactt gccttatcag gaacttgtgt tagtatggct ttaagcagtt ttgtaagttt
     4441 tttaatttta ccgaataata acgtcttaga caaatttaga ttttggcaaa taggtagcct
     4501 tggagcagct acattatctt ctatatctac actactacct tttataattt taggtcactt
     4561 gatagctata tttatttcat cagatttaaa cgctttagct atgggtgatg aaatggctgt
     4621 tggtcttgga gttaatgtta ataggataag atcacttgca ataattgcaa gtgtgctttt
     4681 atgttcaagt attactgcaa ttggtggacc tattggcttc gtaggtctta tagttcctca
     4741 cttttgtggc ttatttataa gcaaagatat acgcacaatg accatttctt catcttttat
     4801 aggtgcagag ctcttgctta tatgtgatat aatcggccgt atgttaggta aaccaggtga
     4861 aattgaagta gggataatta ctgcaataat cgggggtcca gtacttattt atgtaactat
     4921 gaaaaataga ggggttaata actaatgcaa aatttaatta taggtattca aaaaagaaaa
     4981 aatagaataa cactattttc ctcactattt ttattaataa taatcagtct atcatttttc
     5041 attttactta tcggagatga aagttattct ttttcaactt tgattaaagt cttaaatagt
     5101 gaaactgttc ctggagctag tttttcgatt atggaaatta gattaccaaa attattagca
     5161 ggaattatag ctggctggtc ttttggattg gcaggattta tctttcaaac tatgttaaga
     5221 aatcctcttg caagtcctga tataatcggt gtcacaagtt cttcatctat tgcagcggtc
     5281 ttttgcatat tggtattaaa aacaaatagt ttaactactg gaattatttc aataacttgt
     5341 ggactaacat catctttaat attattttta ctagctaaaa aagatggttt ttcagcagca
     5401 agactgataa tattaggtat tggttttcaa gctgtcacaa gagcaggcac ctcattttta
     5461 ttgttgaaag tagcaagata tgaattacaa gaagttatga gatggctcag tggctcttta
     5521 tcttttacaa agttagatga catacctctt gttctaatag taagtattat tgctactata
     5581 ttagttttat tttttaataa aagactagaa attattgaac ttggtgaaga aatagcaatc
     5641 ggacttggag caaatcccga gctttcaagg cttgttttaa ttttttgcgc tgtatcttta
     5701 actgcttttt ctacttcaat tacaggacca atagcttgta tatctttttt agctggtccc
     5761 atagccttaa atattggcaa gaaaagaagc ccaatattag ctggattggt tggaatttta
     5821 ctagttttgt tatcagacat attctctcaa aatattttac cagctagata tccagtaggt
     5881 gttgtaactg gcttgttagg ttcaccatac ttaatatact tactaataaa aatgaacagg
     5941 aggaatatat aatgaaaggt ctgtggtcaa ataatcttac ctgcggttat gatgaaaaaa
     6001 taatcttgga aaatataaat ataaaaatac ctgaagaaaa aatatcagtt attattgggt
     6061 caaatggttg tgggaaatca acactcatta aaaccttgtc tcgacttata aagccattag
     6121 agggagaagt attgcttgat aataaatcaa ttaattctta taaagaaaaa gatttagcaa
     6181 aacacatagc tatattacct caatctccaa taatccctga atcaataaca gtagctgatc
     6241 ttgtaagccg tggtcgtttc ccctacagaa agccttttaa gagtcttgga aaagatgacc
     6301 ttgaaataat aaacagatca atggttaagg ccaatgttga agatctagca aataacctag
     6361 ttgaagaact ttctgggggt caaaggcaaa gagtatggat agctctagcc ctagcccaag
     6421 atacaagtat cctactttta gatgagccaa ctacttactt ggatatctca tatcaaatag
     6481 aactattaga cctcttgact gatctaaacc aaaaatataa gacaaccatt tgcatgattt
     6541 tgcacgatat aaatctaaca gcaagatacg ctgattacct atttgcaatt aaagaaggta
     6601 aacttgttgc agagggaaag cctgaagata tactaaatga taaactagtt aaagatatct
     6661 ttaatcttga agcaaaaatt atacgtgacc ctatttccaa ttcgcctcta atgattccta
     6721 ttggcaagca ccatgttaac tcttaataat tgaatattgg aattcattta aaagtcgcaa
     6781 attttgaact gaccctcaaa agttaggcca aaaaactaac taaaggaggt cagtttttta
     6841 atggcaaata cagcacagaa tttaagataa aattagtaaa agaatatcat gaatgaaaaa
     6901 taggatatag agaataacag caatattaaa aagatcagga ctgataataa acaaaaagaa
     6961 agtacaacga ctagttcaaa agctaaaact tcaagtaaaa agttattcaa gaaaatctag
     7021 aaaatactca tcctacaaag gacaggtagg aaaaatatca gacaacaaaa taaaaagaaa
     7081 tatttattac cgaaaaaaag gggaaatcga gttaagtacc aacaggctca tctattccaa
     7141 aaagatattt actcgatgga aaaactccta gaattagtgc tacaaatatt gacaatggta
     7201 tcttaggtta ctacgaagat atagatgata aaaattatag agtttttgaa aattttatat
     7261 ctgtttcatt tttaggagca gtcttttatc ataaatataa agcaagccta gatatgaaaa
     7321 ttcattgttt aaaattaaaa aataaagaat tgaataaaga agtagcattc tatttaactt
     7381 caattattag gcaagcttta aaaaatactg aatataaaga tcaaatatct agcacagtat
     7441 tgccagatat caaaattaaa ttaccgatag acagtagagg tactccagat tggaattaca
     7501 tggaaagata tagagataga taaagagaga gagggagaga tagataaaaa gtccaccccc
     7561 attttttatg gagaattcaa aaatgtaagg ttaagcaaag aagaatataa aaatctaaaa
     7621 gaaaaattaa actcacacac agatataatg
//
LOCUS       DBDESG010000076         7591 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.076, whole genome shotgun sequence.
ACCESSION   DBDESG010000076 DBDESG010000000
VERSION     DBDESG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.076"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            253..1383
                     /locus_tag="ACKB38_002003"
     CDS             253..1383
                     /locus_tag="ACKB38_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980708.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            1884..2642
                     /locus_tag="ACKB38_002004"
     CDS             1884..2642
                     /locus_tag="ACKB38_002004"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGL7980709.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            2729..3241
                     /locus_tag="ACKB38_002005"
     CDS             2729..3241
                     /locus_tag="ACKB38_002005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGL7980710.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            3423..4367
                     /gene="metA"
                     /locus_tag="ACKB38_002006"
     CDS             3423..4367
                     /gene="metA"
                     /locus_tag="ACKB38_002006"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGL7980711.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            4376..5053
                     /locus_tag="ACKB38_002007"
     CDS             4376..5053
                     /locus_tag="ACKB38_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGL7980712.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            5151..5909
                     /gene="tpiA"
                     /locus_tag="ACKB38_002008"
     CDS             5151..5909
                     /gene="tpiA"
                     /locus_tag="ACKB38_002008"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGL7980713.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            5947..7451
                     /gene="lytC"
                     /locus_tag="ACKB38_002009"
                     /pseudo
     CDS             5947..7451
                     /gene="lytC"
                     /locus_tag="ACKB38_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
BASE COUNT         2392 a         1279 c         1692 g         2228 t
ORIGIN      
        1 gctccataat atccataggg gatttaccca ctacaaatat tatagagccc gaaaatatgg
       61 gaaaactgat ccttgtttct gcttttgtct atagaagaat aataaagatt atcttcttca
      121 aattctccga tattctctaa agttttgtgc aagttgcaca gaacttgttt atttttttgg
      181 tcatcttgcc atagaaatat aaagcgtttt catatataat ataattatca aaagacaaaa
      241 ggagttcacc tcatggtaga attgaatctt aaaaatattt acaaaaaata tccaaacagc
      301 gaacactatt cagttgaaga tttcaacttg aacatcaaag ataaagaatt tatcgttttc
      361 gtaggacctt caggatgtgg taaatcaact acactccgta tgattgctgg tcttgaagac
      421 attacagaag gtactgcatc tatcgatggc gtagttgtca acgacgtagc tccaaaagac
      481 cgtgatatcg ccatggtatt ccaaaactac gctctttacc cacacatgac tgtttatgac
      541 aacatggctt tcggtttgaa attgcgtaaa tacagcaaag aagacattaa caaacgtgtt
      601 caagaagcag ctgaaatact tggattgaaa gaattcttgg aacgtaaacc agctgacctt
      661 tcaggtggtc aacgtcaacg tgttgccatg gggcgtgcga ttgtccgtga tgcgaaagta
      721 ttcttgatgg acgaaccttt gtcaaacttg gatgccaaac ttcgtgtatc aatgcgtgct
      781 gaaatcgcta aaattcaccg tcgtatcgga gctacaacta tctatgtaac tcacgaccaa
      841 acagaagcga tgacacttgc agaccgtatc gttattatgt cagctactaa gaaccctgct
      901 ggtacaggta ctatcggacg tgtagaacaa atcggtactc ctcaagaagt ttacaaaaat
      961 ccagttaaca aattcgttgc aggattcatc ggaagcccag ctatgaactt catcaccgtg
     1021 aaattggttg gtagcgaaat tgtttctgac ggtttccgtt tgaaagtgcc agaaggagca
     1081 ttgaaagttc ttcgtgaaaa aggctacgaa ggaaaagaat tgatctttgg tatccgtcca
     1141 gaagacgtga atgcagaacc tgctttcctt gaaacattcc cagactgtgt tgtaaaagcg
     1201 actatctctg tatcagaact gcttggttca gaatctcacc tttactgtca agttggtaaa
     1261 gacgagtttg ttgcaaaagt tgatgctcgt gactacttgc aaacaggtgc aacagttgag
     1321 cttggatttg acttgaacaa agcacacttc ttcgatgtag aaactgaaaa aacaatctac
     1381 taaaataaat aaaattcaaa gcactacaag aaaagatatc tctttatcaa ttgtagtgga
     1441 gagatatcag ttaatctagg gagagaaaca aaatgcttct ctcctttttg ctagagaagt
     1501 catattatgc atctatattg tgatgctctt taatactctt cgaaaatctc ttcaaaccac
     1561 gtcaacgtcg ccttgccgta cgtatgatta ctgatttcgt cagttttatc tgcaacctca
     1621 aagatgtact ttgagcagct tacggctagt ttcctagttt gctctttgat ttccattgag
     1681 tattatttgt gggtaccatc tacaagtgaa gctatatgcg taaactacgt gagcaattga
     1741 attcgaacta gagaggtaat aataaattta tgctatagtt atggtgactt gtatgctttt
     1801 gattctagtt tatcaaataa tagattagaa ttgtcagata atatcatttt gtgttataat
     1861 gaagaaaaaa cagaggtgtt caaatgtcag aagcaggtca taagttttta gcaaaattgg
     1921 ggaaaaaacg cttacgtcca ggtggaaagc gtgccacaga ttggttaatt gcagaaggag
     1981 gattttcaaa agaaaagaga atactagagg ttgcgtgtaa taggggaact acagcaattg
     2041 agttggcaca gcgttttggt tgcaagataa ctgctgttga tatggatgct caagctttag
     2101 aagtggctaa aaaatctgct ggaacggcag gtgttgctca tttaatcagt tttgaaagag
     2161 caaatgcaat gaaacttcct tatcaagatg ctagttttga tattgttata aatgaagcta
     2221 tgctgactat gcaagccgat caagctaaga aaaaatgtgt aatggaatat ctaagggtat
     2281 taaaacctgg aggtcttctc ttgacacatg atgtgcttct taaggaagct aaagagtcta
     2341 tcagacagga attatcacaa gcaattcatg taaatgtagg tcctttaact caagatggtt
     2401 gggaacaggt gatgatagaa tcaggttatt gtgatgtgaa agcattgact ggtgaaatga
     2461 cattaatgaa attatcgggt atgatttatg acgaaggttt gctaggaact ttgaaaattt
     2521 gtgtaaatgc ttgtaaaaag gagaatagaa agcagttttt aactatgtat aaaatgtttg
     2581 ctaagaataa acagaaattg ggctttattg cgatggctag ttataaatcg tcaaaacgtt
     2641 agataattat tgaagttaac ttttcctttt ttctttctta aaaaatatgc tataatagag
     2701 agtaaaaaac tttgaaagaa agaaaaagat gaatttaaaa gattacattg caacaattga
     2761 aaattatcca aaggaaggca ttaccttccg tgatattagt cctttgatgg ctgatggaaa
     2821 tgcttatagc tacgctgttc gtgaaatcgt tcagtatgct actgacaaga aagtcgacat
     2881 gatcgtggga cctgaagctc gtggatttat cgtgggttgt ccagttgcct ttgagttggg
     2941 aattggtttt gcgcctgttc gtaagccagg taaattgcca cgcgaagtta tttctgctga
     3001 ctatgaaaaa gagtacggtg tcgatacctt gactatgcac gcggatgcca ttaagccagg
     3061 tcaacgtgtt cttattgtag atgacctttt ggcgacaggt ggaactgtta aggcaactat
     3121 cgagatgatt gaaaaacttg gtggtgttat ggcaggttgt gccttccttg ttgaattgga
     3181 tgaattgaac ggccgtgaaa aaattggtga ctacgactac aaagttctta tgcattatta
     3241 atgaaaacag tccctagggc tgttttctct acactaggat ataaaaatag actataacta
     3301 gttagagaaa aactataatt gaaaactata tcttcttgca gtataataaa aggactaagt
     3361 gtttgagatt tgtcttcaaa catatgcaat tattcctgaa agagtacagt taggagaggg
     3421 ttatgccgat tcgaattgat aaaaaattgc cagctgttga gattttacgg acagagaata
     3481 tctttgtcat ggatgatcaa cgtgctgccc accaagatat ccgtcctttg aagattttaa
     3541 ttttaaatct catgccacag aaaatggtca cagagaccca gttgttgcgc cacttggcta
     3601 atacacccct acaactggat attgattttc tctatatgga gagccaccgt tctaaaacaa
     3661 ctcgttcaga gcacatggag accttctata aaacttttcc tgaagtcaag gatgagtatt
     3721 ttgatgggat gatcatcacg ggtgctccag ttgagcattt accatttgag gaagtggact
     3781 attgggagga atttagacag atgcttgagt ggtctaagac tcatgtctat tcgacccttc
     3841 atatctgttg gggggctcag gctgggcttt atctgcgcta tggtgtagaa aaataccaga
     3901 tggacagtaa gctatcaggt atttatcctc aggacaccct aaaagagggt caccttctat
     3961 ttagaggctt tgatgatagc tatgtatccc ctcattcacg gcacacggag atttctaagg
     4021 aagaggtctt aaacaagacc aatctcgaga ttttatcaga aggacctcag gttggggttt
     4081 ctattttggc cagtcgtgat ttacgagaaa tttatagttt tggtcatttg gagtatgacc
     4141 gtgatacctt ggcaaaagag tattttcgag atcgtgatgc aggtttcgac ccacatattc
     4201 cagaaaatta ctttaaagat gatgatgtta atcaggtacc ttgtctttgt tggtcttcat
     4261 ctgcagccct ctttttcagt aattgggtaa accatgcggt ctatcaggag acgccttttg
     4321 attggagaaa gatagaagat gatgcatctg catatgggta tttataagag gaattatgac
     4381 atatttagac gcttttaaat caggtacctt ggttttaccg agtgccctgc tcttgcattt
     4441 taaggaactc tttccttcta gcgacgattt tctggtttgg caatttttct atttgcaaaa
     4501 tacgacaggc ttagaagaaa tgtcgccaag ccagattgct gaaaggattg gcaaggaaat
     4561 ttcggatgtc aaccagtcca tttctaatct gacggaaagg ggactgctcc agtatcgtac
     4621 tatcgaatta aatggcgaaa ttgaattgct ctttgatgct agtttggcct tggaacgttt
     4681 ggatgacctg tttggagcag ttcattcaag ttcagaccag ctaacacctc aaaaccagct
     4741 caaggatttg gtggaaacct tccagcagga gttgggacga ttgttgacgc cttttgagat
     4801 tgaggatttg accaagacac taaaggaaga tggaaccagt gctgacttga ttaaggaggc
     4861 tcttcgtgaa gctgttttga atggaaaacc aaactggaag tacattcagg cgattttgag
     4921 aaactggcgc catgaaggaa tcaagagtgt ggctcaaatt gaggccaaga gagcagaaag
     4981 agaagcaagc aatcctcagt tgacacaggt atctgcagat ttcataaatg ccatggatct
     5041 ctggaaggat taatccatgc aagtaggctt gaaatccgag taagatttgc aagctgtgta
     5101 taattgtgat agaataaata gaaaataaat tgaaaaaaga ggtatgtgaa atgtcacgta
     5161 aaccatttat cgctggtaac tggaaaatga acaaaaatcc agaagaagct aaagcattcg
     5221 ttgaagcagt tgcatcaaaa cttccttcat cagatcttgt tgaagcaggt atcgctgctc
     5281 cagctcttga tttgacaact gttcttgctg ttgcaaaagg ctcaaacctt aaagttgctg
     5341 ctcaaaactg ctactttgaa aatgcaggtg ctttcactgg tgaaactagc ccacaagttt
     5401 tgaaagaaat cggtactgac tacgttgtta tcggtcactc agaacgccgt gactacttcc
     5461 atgaaactga tgaagatatc aacaaaaaag caaaagcaat ctttgcgaac ggtatgcttc
     5521 caatcatctg ttgtggtgaa tcacttgaaa cttacgaagc tggtaaagct gctgaattcg
     5581 taggtgctca agtatctgct gcattggctg gattgactgc tgaacaagtt gctgcctcag
     5641 ttatcgctta tgagccaatc tgggctatcg gtactggtaa atcagcttca caagacgatg
     5701 cacaaaaaat gtgtaaagtt gttcgtgacg ttgtagctgc tgactttggt caagaagtcg
     5761 cagacaaagt tcgtgttcaa tacggtggtt ctgttaaacc tgaaaatgtt gcttcataca
     5821 tggcttgccc agacgttgac ggtgcccttg taggtggtgc gtcacttgaa gctgaaagct
     5881 tcttggcttt gcttgacttt gtaaaataat cagtaagtag caaaagctag gtggaacagc
     5941 attcagatgt ctgttacatt ttttatagga gagaaagatt gaaaacaaaa attggattag
     6001 caagtatctg tttactaggc ttggcaacta gtcatgtcgc tgcaaatgaa actgaagtag
     6061 caaaaacttc gcaggataca acgacagctt caagtagttc agagcaaaat cagtcttcta
     6121 ataaaacgca aacgagcgca gaagtacaga ctaatgctgc tgcccactgg gatggggatt
     6181 attatgtaaa ggatgatggt tctaaagctc aaagtgaatg gatttttgac aactactata
     6241 aggcttggtt ttatattaat tcagatggtc gttactcgca gaatgaatgg catggaaatt
     6301 actacctgaa atcaggtgga tatatggccc aaaacgagtg gatctatgac agtaattaca
     6361 agagttggtt ttatctcaag tcagatgggg cttatgctca tcaagaatgg caattgattg
     6421 gaaataagtg gtactacttc aagaagtggg gttacatggc taaaagccaa tggcaaggaa
     6481 gttatttctt gaatggtcaa ggagctatga tgcaaaatga atggctctat gatccagcct
     6541 attctgctta tttttatcta aaatccgatg gaacttatgc taaccaagag tggcaaaaag
     6601 tgggcggcaa atggtactat ttcaagaagt ggggctatat ggctcggaat gagtggcaag
     6661 gcaactacta tttgactgga agtggtgcca tggcgactga cgaagtgatt atggatggta
     6721 ctcgctatat ctttgcggcc tctggtgagc tcaaagaaaa aaaagatttg aatgtcggct
     6781 gggttcacag agatggtaag cgctatttct ttaataatag agaagaacaa gtgggaaccg
     6841 aacatgctaa gaaagtcatt gatattagtg agcacaatgg tcgtatcaat gattggaaaa
     6901 aggttattga tgagaacgaa gtggatggtg tcattgttcg tctaggttat agcggtaaag
     6961 aagacaagga attggcgcat aacattaagg agttaaaccg tctgggaatt ccttatggtg
     7021 tctatctcta tacctatgct gaaaatgaga ccgatgctga gagtgacgct aaacagacca
     7081 ttgaacttat aaagaaatac aatatgaacc tgtcttaccc tatctattat gatgttgaga
     7141 attgggaata tgtaaataag agcaagagag ctccaagtga tacaggcact tgggttaaaa
     7201 tcatcaacaa gtacatggac acgatgaagc aggcgggtta tcaaaatgtg tatgtctata
     7261 gctatcgtag tttattacag acgcgtttaa aacacccaga tattttaaaa catgtaaact
     7321 gggtagcggc ctatacgaat gctttagaat gggaaaaccc tcattattca ggaaaaaaag
     7381 gttggcaata tacctcttct gaatacatga aaggaatcca agggcgcgta gatgtcagcg
     7441 tttggtatta agcgatgatt tgaaagaggg atgtgatagt agcaccctct ttttctttgt
     7501 tttatgatag ttcatcctcg agtaaattca agttcttgct cggaaatgaa gcttatatag
     7561 tagattgaat atagacaaat accttgtgat t
//
LOCUS       DBDESG010000077         7095 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.077, whole genome shotgun sequence.
ACCESSION   DBDESG010000077 DBDESG010000000
VERSION     DBDESG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.077"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..1397
                     /gene="recG"
                     /locus_tag="ACKB38_002010"
     CDS             <1..1397
                     /gene="recG"
                     /locus_tag="ACKB38_002010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGL7980714.1"
                     /translation="KFEELFYFQMQLQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPF
                     ALTQAQEKSLQEILTDMKSDHHMNRLLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVP
                     TEILAEQHFESLQNLFPNLKLALLTGSLKAAEKREVLETIAKGEADLIIGTHALIQDG
                     VEYARLGLIIIDEQHRFGVGQRRILREKGDNPDVLMMTATPIPRTLAITAFGDMDVSI
                     IDQMPAGRKPIVTRWIKHEQLPQVLTWLEGEIQKGSQVYVISPLIEESEALDLKNAIA
                     LSEELTTHFAGKAEVALLHGRMKSDEKDQIMQDFKERKTDILVSTTVIEVGVNVPNAT
                     VMIIMDADRFGLSQLHQLRGRVGRGDKQSYAVLVANPKTDSGKDRMRIMTETTNGFVL
                     AEEDLKMRGSGEIFGTRQSGLPEFQVADIIEDFPILEEARKVASYISSIEAWQEDPEW
                     RMIALHLEKKEHLD"
     gene            1425..1574
                     /locus_tag="ACKB38_002011"
     CDS             1425..1574
                     /locus_tag="ACKB38_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980715.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            1912..2892
                     /locus_tag="ACKB38_002012"
     CDS             1912..2892
                     /locus_tag="ACKB38_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGL7980716.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            3653..3880
                     /locus_tag="ACKB38_002013"
     CDS             3653..3880
                     /locus_tag="ACKB38_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980717.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            4168..7065
                     /gene="nanA"
                     /locus_tag="ACKB38_002014"
     CDS             4168..7065
                     /gene="nanA"
                     /locus_tag="ACKB38_002014"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGL7980718.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKKDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         2264 a         1267 c         1627 g         1937 t
ORIGIN      
        1 taaagtttga ggaactcttt tatttccaaa tgcagctgca gatgctcaag tctgaaaata
       61 gagttcaggg aagtggtctg gttctgaatt ggtctcagga aaaagtgaca gcagttaaag
      121 taagtcttcc ttttgccctg acccaagctc aggaaaagag tttgcaggaa attttaactg
      181 atatgaagtc cgaccaccac atgaatcgtc tcctacaagg ggatgtgggg agtggaaaaa
      241 cggtagtcgc tggcttggcc atgtttgcgg cagtgacagc aggttatcag gctgccctaa
      301 tggtaccaac agaaatcctc gcagagcaac actttgagag tttacagaac ctttttccca
      361 atttgaaact ggctctcttg acaggttcct tgaaagctgc agaaaagaga gaagtcttgg
      421 agaccattgc caagggtgag gctgatttga ttataggaac tcacgctctg atacaagatg
      481 gggtggagta tgctcgtctt ggtttgatta ttatcgatga gcagcaccgt tttggtgtag
      541 ggcaaaggcg tattttacgg gaaaaaggtg acaatccaga tgtcctcatg atgacggcga
      601 ctcccattcc acggacgctt gccatcacag cctttggaga tatggatgtt tccattatcg
      661 accagatgcc agcaggtcgg aagcctattg tgacgcgctg gatcaaacat gagcaactac
      721 ctcaggtctt gacttggtta gagggggaaa ttcaaaaagg ttcccaagtc tatgtcatct
      781 ctcctttgat tgaagaatca gaagctctag atttgaaaaa tgccattgcc ttatcagagg
      841 agttgacgac tcattttgca ggcaaggcag aggtggctct tctacatggt aggatgaaga
      901 gtgacgaaaa agaccagatc atgcaggatt tcaaggagag aaagacggat attctggttt
      961 cgacgacggt tattgaggtt ggggtcaacg ttcccaatgc gactgtcatg attatcatgg
     1021 atgccgatcg cttcggtctc agtcaacttc accagcttag aggtcgtgtc ggtcgggggg
     1081 acaagcagtc ctacgctgtt ctcgttgcta atcccaagac ggattctggg aaagaccgca
     1141 tgcgtatcat gacagaaacg accaatggat ttgtccttgc ggaggaagat ttgaaaatgc
     1201 gtggttctgg tgagattttt ggaaccagac agtcaggact tccagagttc caagtggctg
     1261 atattatcga agattttccg attttagaag aagcaagaaa ggttgctagc tacattagtt
     1321 ctatagaagc ttggcaagaa gatccagagt ggcgcatgat tgcccttcat ctggaaaaga
     1381 aagaacatct ggattaagct ttctctaagg aaaacttata ctcaatgaaa atcaaagagc
     1441 aaactaggaa gctaaccgca ggttgctcaa aacactgttt tgaggttgtg gatgaaactg
     1501 acgaagtcag ctcaaaacac cgttttgagg tggcagatag aactgacgaa gtcagtaaca
     1561 tatatatacg gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtattaagct
     1621 agtttttagg tttggctctt atactagagt catcaaaaag aaacgaggac tctcatatga
     1681 cagtaacgat taaagtaaat taccaaacca ctttccaaaa gaaggaagca aaaaactagt
     1741 ataaacagaa gagagagcga aatgctcttt tttcgtttct aaaactactt tcagcccatc
     1801 atcctaaaag taaagaatct aaattcactt tctatttacc cttctttctt gcattgatta
     1861 catagatatg ctacagttgt ggtaacgatt acaaaataaa aggagcatgc tatgaaaaat
     1921 ccagctttgc tagaagaaat taagacctat agaggaaggg atgaggttcc ggaagacttt
     1981 gatgatttct gggatgggga agtgaaaaat gtttccacgc ttccatccta ccacttggag
     2041 gaaagagatt tccacattcc tcaagtcaag tgctatgagt taacatttga aggaagcaag
     2101 gaaggaaagg tctatgcacg cattgttctt ccaaagagtg aggagaaggt cccattaatc
     2161 ttccattttc atggttatat gggacgtggc tgggactggg ccgacatgct gggcttcacc
     2221 gtagctggtt acggtgttgt ttccatggat gtgcggggcc agtcaggtta ctcacaagac
     2281 ggcttgcgtt ctcctttagg aaataccgtg aaggggcata ttatccgtgg tgctgtggaa
     2341 ggtcgggacc acctctttta taaggatgtt tatctggata tttaccagtt ggtcgaaatt
     2401 gttgctagtc tgtctcaggt tgatgagaag cgtctttcta gctatggtgc ctcacaagga
     2461 ggggctctag ctctagttgc agcagcgctc aatcctcgaa ttcagaaaac agttgccatt
     2521 tatcccttct tgtcagactt cagacgggtg attgagattg gtaatactag cgaggcttac
     2581 gacgaacttt tccgttattt caagtttcac gaccccttcc atgaaacaga ggaggaaatc
     2641 atggcgaccc ttgcctatat cgatgtcaaa aatcttgccc atcgtatcca aggtgaggtt
     2701 aagatgatta cgggcttgga cgacgatgtt tgctatccca ttacccagtt tgcgatttat
     2761 aatcgtctga cctgcgataa aacctatcgc atcatgcctg agtatgctca cgaagccatg
     2821 aatgtatttg tcaatgacca agtctacaac tggctctgtg gaagtgagat tccttttaaa
     2881 tatctaaaat aaggagtcga ctctaagcac aaaatcttaa aaattacaaa cacgcatagt
     2941 atcaggggat taagaaaact ttatactatg cgttttatca tggaaatata gtaaaatgaa
     3001 ataagaacag gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaaac
     3061 aaatgtgtac tattctagtg tcaatctatt atatttatag aattttttgt tgctagattt
     3121 gtcaaattgc ttaaaataat ttttttcaga aagcaaaagc cgatacctat cgagtagggt
     3181 agttcttgct atcgtcaggc ttgtctgtag gtgttaatac ttttcaaaaa tctcttcaaa
     3241 ccacgtcagc ttcgccttgc cgtaggtatg gttactgact tcgtcagttc tatccacaac
     3301 ctcaaaacag tgttttgagc atcatgcggc tagcttctta gtttgctctt tgattttcat
     3361 tgagtataaa aacagatgag tttctgtttt ctttttatgg actataaatg ttcagctgaa
     3421 actactttca aggacattat tatataaaag aattttttga aactaaaatc tactatatta
     3481 cactatattg aaagcgtttt aaaaatgagg tataataaat ttactaacgc ttataaaaag
     3541 tgatagaatc tatttttatg tatatttaaa gatagattgc tgtaaaaata gtagtagcta
     3601 tgcgaaataa cagatagaga gaagggattg aagcttagaa aaggggaata atatgatatt
     3661 taaggcattc aagacaaaaa agcagagaaa aagacaagtt gaactacttt tgacagtttt
     3721 tttcgacagt tttctgattg atttatttct tcacttattt gggattgtcc cctttaagct
     3781 ggataagatt ctgattgtga gcttgattat atttcccatt atttctacaa gtatttatgc
     3841 ttatgaaaag ctatttgaaa aagtgttcga taaggattga gcaggaagta tggtgtaaat
     3901 agcataggct gatgtccatc atttgcttat aaagagatat tttagtttaa ttgcagcggt
     3961 gtcctggtag ataaactaga ttggcaggag tctgattgga gaaaggagag gggaaaattg
     4021 gcaccaattt gagatagttt gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa
     4081 gaaagttaat aaaagacaaa ctaagtgcat tttctggagt aaatgtctta tttcagaaat
     4141 cgggatatag atatagagag gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt
     4201 tatagcatta ggaaactatc ggtaggagcg gtttctatga ttgtaggagc agtggtattt
     4261 ggaacgtctc ctgttttagc tcaagaaggg gcaagtgagc aacctctggc aaatgaaact
     4321 caactttcgg gggagagctc aaccctaact gatacagaaa agagccagcc ttcttcagag
     4381 actgaacttt ctggcaataa gcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt
     4441 ccaagagatt actatgcacg agatttggaa aatgtcgaaa cagtgataga aaaagaagat
     4501 gttgaaacca atgcttcaaa tggtcagaga gttgatttat caagtgaact agataaacta
     4561 aagaaacttg aaaacgcaac agttcacatg gagtttaagc cagatgccaa ggccccagca
     4621 ttctataatc tcttttctgt gtcaagtgct actaaaaaag atgagtactt cactatggca
     4681 gtttacaata atactgctac tctagagggg cgtggttcgg atgggaaaca gttttacaat
     4741 aattacaacg atgcaccctt aaaagttaaa ccaggtcagt ggaattctgt gactttcaca
     4801 gttgaaaaac cgacagcaga actacctaaa ggccgagtgc gcctctacgt aaacggggta
     4861 ttatctcgaa caagtctgag atctggcaat ttcattaaag atatgccaga tgtaacgcat
     4921 gtgcaaatcg gagcaaccaa gcgtgccaac aatacggttt gggggtcaaa tctacagatt
     4981 cggaatctca ctgtgtataa tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa
     5041 ctttttaaac gctcagattt agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa
     5101 acggacatat tcgaaagcgg gcgtaacggt aacccaaata aagatggaat caagagttat
     5161 cgtattccag cacttctcaa gacagataaa ggaactttga tcgcaggtgc agatgaacgc
     5221 cgtctccatt cgagtgactg gggtgatatc ggtatggtca tcagacgtag tgaagataat
     5281 ggtaaaactt ggggtgaccg agtaaccatt accaacttac gtgacaatcc aaaagcttct
     5341 gacccatcga tcggttcacc agtgaatatc gatatggtgt tggttcaaga tcctgaaacc
     5401 aaacgaatct tttctatcta tgacatgttc ccagaaggga agggaatctt tggaatgtct
     5461 tcacaaaaag aagaagccta caaaaaaatc gatggaaaaa cctatcaaat cctctaccgt
     5521 gaaggagaaa agggagctta taccattcga gaaaatggta ctgtctatac accagatggt
     5581 aaggcgacag actatcgcgt tgttgtagat cctgttaaac cagcctatag cgacaagggt
     5641 gatctataca agggtgacca attactagga aatatctact tcacaacaaa caaaacttct
     5701 ccatttagaa ttgccaagga tagctatcta tggatgtcct acagtgatga cgacgggaag
     5761 acatggtcag ctcctcaaga tattactccg atggtcaaag ccgattggat gaaattcttg
     5821 ggtgtaggtc ctggaacagg aattgtactt cggaatgggc ctcacaaggg acggattttg
     5881 ataccggttt atacgactaa taatgtatct cacttagatg gctcgcaatc ttctcgtgtc
     5941 atctattcag atgatcatgg aaaaacttgg catgctggag aagcggtcaa cgataaccgt
     6001 caggtagacg gtcaaaagat ccactcttct acgatgaaca atagacgtgc gcaaaataca
     6061 gaatcaacgg tggtacaact aaacaatgga gatgttaaac tctttatgcg tggtttgact
     6121 ggagatcttc aggttgctac aagtaaagac ggaggagtga cttgggagaa ggatatcaaa
     6181 cgttatccac aggttaaaga tgtctatgtt caaatgtctg ctatccatac gatgcacgaa
     6241 ggaaaagaat acatcatcct cagtaatgca ggtggaccga aacgtgaaaa tgggatggtc
     6301 cacttggcac gtgtcgaaga aaatggtgag ttgacttggc tcaaacacaa tccaattcaa
     6361 aaaggagagt ttgcctataa ttcgctccaa gaattaggaa atggggagta tggcatcttg
     6421 tatgaacata ctgaaaaagg acaaaatgcc tataccctat catttagaaa atttaattgg
     6481 gaatttttga gcaaaaatct gatttctcct accgaagcga aagtgaagcg aactagagag
     6541 atgggcaaag gagagatggg caaaggagtt attggcttgg agttcgactc agaagtattg
     6601 gtcaacaagg ctccaaccct tcaattggca aatggtaaaa cagcgacttt cctaacccag
     6661 tatgatagca agaccttgtt gtttgcagta gataaggaag atatcggaca ggaaattatt
     6721 ggtatagcta aaggaagcat cgaaagtatg cataatcttc ctgtaaatct agcaggtgcc
     6781 agagttcctg gcggagtaaa tggtagcaaa gcagcggtgc atgaagttcc agaatttaca
     6841 gggggagtta atggtacaga gccagctgtt catgaaatcg cagagtataa gggatctgat
     6901 tcgcttgtaa ctcttactac aaaaaaagat tatacttaca aagctcctct tgctcagcag
     6961 gcacttcctg aaacaggaaa caaggagagt gacctcctag cttcactagg actaacagct
     7021 ttcttccttg gtctgtttac gctagggaaa aagagagaac aataagagaa gaattctaaa
     7081 catttgattt tgtaa
//
LOCUS       DBDESG010000078         7004 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.078, whole genome shotgun sequence.
ACCESSION   DBDESG010000078 DBDESG010000000
VERSION     DBDESG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.078"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            290..6712
                     /locus_tag="ACKB38_002015"
     CDS             290..6712
                     /locus_tag="ACKB38_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGL7980719.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     AENKEKHENIHSAMETSQDFKEKKTAVIKEKEVVSKNPVIDNNTSNEEAKIKEENSNK
                     SQGDYTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIF
                     NGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKSKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRMEEYDYKYDDKGNIIAYDDG
                     TDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKAT
                     KYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSE
                     YPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALK
                     VTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVL
                     MSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVE
                     AIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRV
                     NESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVY
                     LQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGF
                     DGEIQEQHIYINGKEYTSFNDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTGEVS
                     ELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGY
                     VINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREE
                     HSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFL
                     GLKKKNQD"
BASE COUNT         2755 a          938 c         1349 g         1962 t
ORIGIN      
        1 caaaacactg ttttgagcaa tctgcggcta gtttcctagt ttgctctttg attttcattg
       61 agtactaatc ttatttttta atgtgtgcta aaaaataaat taactaatgt taattttttt
      121 gacttaaatt tttttaaatg atatactgtt tttatagaga gataacaatt atatataaat
      181 gtactactct atttcctaga taatacttag taaaactttt tataacaaag gctagcaaag
      241 ttaaagttta ttatctattg gaagttatat aaatatgtaa ggaattaaaa tgaaaaaaag
      301 tacagtattg tcattaacca cagctgcagt tattttagca gcctatgccc ctaatgaggt
      361 agtcttagca gacacatcta gctctgaaga tgctttaaac atctctgata aagaaaaagt
      421 agcagaaaat aaagagaaac atgaaaatat ccatagtgct atggaaactt cacaggattt
      481 taaagagaag aaaacagcag tcattaagga aaaagaagtt gttagtaaaa atcctgtgat
      541 agacaataac actagcaatg aagaagcaaa aatcaaagaa gaaaattcca ataaatccca
      601 aggagattat acggactcat ttgtgaataa aaacacagaa aatcccaaaa aagaagataa
      661 agttgtctat attgctgaat ttaaagataa agaatctgga gaaaaagcaa tcaaggaact
      721 atccagtctt aagaatacaa aagttttata tacttatgat agaattttta acggtagtgc
      781 catagaaaca actccagata acttggacaa aattaaacaa atagaaggta tttcatcggt
      841 tgaaagggca caaaaagtcc aacccatgat gaatcatgcc agaaaggaaa ttggagttga
      901 ggaagctatt gattacctaa agtctatcaa tgctccgttt gggaaaaatt ttgatggtag
      961 aggtatggtc atttcaaata tcgatactgg aacagattat agacataagg ctatgagaat
     1021 cgatgatgat gccaaagcct caatgagatt taaaaaagaa gacttaaaag gcactgataa
     1081 aaattattgg ttgagtgata aaatccctca tgcgttcaat tattataatg gtggcaaaat
     1141 cactgtagaa aaatatgatg atggaaggga ttattttgac ccacatggga tgcatattgc
     1201 agggattctt gctggaaatg atactgaaca agacatcaaa aactttaacg gcatagatgg
     1261 aattgcacct aatgcacaaa ttttctctta caaaatgtat tctgacgcag gatctgggtt
     1321 tgcgggtgat gaaacaatgt ttcatgctat tgaagattct atcaaacaca acgttgatgt
     1381 tgtttcggta tcatctggtt ttacaggaac aggtcttgta ggtgagaaat attggcaagc
     1441 tattcgggca ttaagaaaag caggcattcc aatggttgtc gctacgggta actatgcgac
     1501 ttctgcttca agttcttcat gggatttagt agcaaataat catctgaaaa tgaccgacac
     1561 tggaaatgta acacgaactg cagcacatga agatgcgata gcggtcgctt ctgctaaaaa
     1621 tcaaacagtt gagtttgata aagttaacat aggtggagaa agttttaaat acagaaatat
     1681 aggggccttt ttcgataaga gtaaaatcac aacaaatgaa gatggaacaa aagctcctag
     1741 taaattaaaa tttgtatata taggcaaggg gcaagaccaa gatttgatag gtttggatct
     1801 taggggcaaa attgcagtaa tggatagaat ttatacaaag gatttaaaaa atgcttttaa
     1861 aaaagctatg gataagggtg cacgcgccat tatggttgta aatactgtaa attactacaa
     1921 tagagataat tggacagagc ttccagctat gggatatgaa gcggatgaag gtactaaaag
     1981 tcaagtgttt tcaatttcag gagatgatgg tgtaaagcta tggaacatga ttaatcctga
     2041 taaaaaaact gaagtcaaaa gaaataataa agaagatttt aaagataaat tggagcaata
     2101 ctatccaatt gatatggaaa gttttaattc caacaaaccg aatgtaggtg acgaaaaaga
     2161 gattgacttt aagtttgcac ctgacacaga caaagaactc tataaagaag atatcatcgt
     2221 tccagcagga tctacatctt gggggccaag aatagattta cttttaaaac ccgatgtttc
     2281 agcacctggt aaaaatatta aatccacgct taatgttatt aatggcaaat caacttatgg
     2341 ctatatgtca ggaactagta tggcgactcc aatcgtggca gcttctactg ttttgattag
     2401 accgaaatta aaggaaatgc ttgaaagacc tgtattgaaa aatcttaagg gagatgacaa
     2461 aatagatctt acaagtctta caaaaattgc cctacaaaat actgcgcgac ctatgatgga
     2521 tgcaacttct tggaaagaaa aaagtcaata ctttgcatca cctagacaac agggagcagg
     2581 cctaattaat gtggccaatg ctttgagaaa tgaagttgta gcaactttca aaaacactga
     2641 ttctaaaggt ttggtaaact catatggttc catttctctt aaagaaataa aaggtgataa
     2701 aaaatacttt acaatcaagc ttcacaatac atcaaacaga cctttgactt ttaaagtttc
     2761 agcatcagcg ataactacag attctctaac tgacagatta aaacttgatg aaacatataa
     2821 agatgaaaaa tctccagatg gtaagcaaat tgttccagaa attcacccag aaaaagtcaa
     2881 aggagcaaat atcacatttg agcatgatac tttcactata ggcgcaaatt ctagctttga
     2941 tttgaatgcg gttataaatg ttggagaggc caaaaacaaa aataaatttg tagaatcatt
     3001 tattcatttt gagtcagtgg aagaaatgga agctctaaac tccaacggga agaaaataaa
     3061 cttccaacct tctttgtcga tgcctctaat gggatttgct gggaattgga accacgaacc
     3121 aatccttgat aaatgggctt gggaagaagg gtcaagatca aaaacactgg gaggttatga
     3181 tgatgatggt aaaccgaaaa ttccaggaac cttaaataag ggaattggtg gagaacatgg
     3241 tatagataaa tttaatccag caggagttat acaaaataga aaagataaaa atacaacatc
     3301 cctggatcaa aatccagaat tatttgcttt caataacgaa gggatcaacg ctccatcatc
     3361 aagtggttct aagattgcta acatttatcc tttagattca aatggaaatc ctcaagatgc
     3421 tcaacttgaa agaggattaa caccttctcc acttgtatta agaagtgcag aagaaggatt
     3481 gatttcaata gtaaatacaa ataaagaggg agaaaatcaa agagacttaa aagtcatttc
     3541 gagagaacac tttattagag gaattttaaa ttctaaaagc aatgatgcaa agggaatcaa
     3601 atcatctaaa ctaaaagttt ggggtgactt gaagtgggat ggactcatct ataatcctag
     3661 aggtagagaa gaaaatgcac cagaaagtaa ggataatcaa gatcctgcta ctaagataag
     3721 aggtcaattt gaaccgattg cggaaggtca atatttctat aaatttaaat atagattaac
     3781 taaagattac ccatggcagg tttcctatat tcctgtaaaa attgataaca ccgcccctaa
     3841 gattgtttcg gttgattttt caaatcctga aaaaattaag ttgattacaa aggatactta
     3901 tcataaggta aaagatcagt ataagaatga aacgctattt gcgagagatc aaaaagaaca
     3961 tcctgaaaaa tttgacgaga ttgcgaacga agtttggtat gctggcgccg ctcttgttaa
     4021 tgaagatgga gaggttgaaa aaaatcttga agtaacttac gcaggtgagg gtcaaggaag
     4081 aaatagaaaa cttgataaag acggaaatac catttatgaa attaaaggtg cgggagattt
     4141 aaggggaaaa atcattgaag tcattgcatt agatggttct agcaatttca caaagattca
     4201 tagaattaaa tttgctaatc aggctgatga aaaggggatg atttcctatt atctagtaga
     4261 tcctgatcaa gattcatcta aatatcaaaa gcttggcgag attgcagaat ctaaatttaa
     4321 aaatttagga aatggaaaag agggtagtct aaaaaaagat acaactgggg tagaacatca
     4381 tcatcaagaa aatgaagagt ctattaaaga aaaatctagt tttactattg atagaaatat
     4441 ttcaacaatt agagactttg aaaataaaga cttaaagaaa ctcattaaaa agaaatttag
     4501 agaagttgat gattttacaa gtgaaactgg taagagaatg gaggaatacg attataaata
     4561 cgatgataaa ggaaatataa tagcctacga tgatgggact gatctagaat atgaaactga
     4621 gaaacttgac gaaatcaaat caaaaattta tggtgttcta agtccgtcta aagatggaca
     4681 ctttgaaatt cttggaaaga taagtaatgt ttctaaaaat gccaaggtat attatgggaa
     4741 taactataaa tctatagaaa tcaaagcgac caagtatgat ttccactcaa aaacgatgac
     4801 atttgatcta tacgctaata ttaatgatat tgtggatgga ttagcttttg caggagatat
     4861 gagattattt gttaaagata atgatcagaa aaaagctgaa attaaaatta gaatgcctga
     4921 aaaaattaag gaaactaaat cagaatatcc ctatgtatca agttatggga atgtcataga
     4981 attaggggaa ggagatcttt caaaaaacaa accagacaat ttaactaaaa tggaatctgg
     5041 taaaatctat tctgattcag aaaaacaaca atatctgtta aaggataata tcattctaag
     5101 aaaaggctat gcactaaaag tgactaccta taatcctgga aaaacggata tgttagaagg
     5161 aaatggagtc tatagcaagg aagatatagc aaaaatacaa aaggccaatc ctaatctaag
     5221 agccctttca gaaacaacaa tttatgctga tagtagaaat gttgaagatg gaagaagtac
     5281 ccaatctgta ttaatgtcgg ctttggacgg ctttaacatt ataaggtatc aagtgtttac
     5341 atttaaaatg aacgataaag gggaagctat cgataaagac ggaaatcttg tgacagattc
     5401 ttctaaactt gtattatttg gtaaggatga taaagaatac actggagagg ataagttcaa
     5461 tgtagaagct ataaaagaag atggctccat gttatttatt gataccaaac cagtaaacct
     5521 ttcaatggat aagaactact ttaatccatc taaatctaat aaaatttatg tacgaaatcc
     5581 agaattttat ttaagaggta agatttctga taagggtggt tttaactggg aattgagagt
     5641 taatgaatcg gttgtagata attatttaat ctacggagat ttacacattg ataacactag
     5701 agattttaat attaagctga atgttaaaga cggtgacatc atggactggg gaatgaaaga
     5761 ctataaagca aacggatttc cagataaggt aacagatatg gatggaaatg tttatcttca
     5821 aactggctat agcgatttga atgctaaagc agttggagtc cactatcagt ttttatatga
     5881 taatgttaaa cccgaagtaa acattgatcc taagggaaat actagtatcg aatatgctga
     5941 tggaaaatct gtagtcttta acatcaatga taaaagaaat aatggattcg atggtgagat
     6001 tcaagaacaa catatttata taaatggaaa agaatataca tcatttaatg atattaaaca
     6061 aataatagac aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac
     6121 cgtaaaagaa ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag
     6181 ggtaactgtg accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga
     6241 ttttatttta cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga
     6301 aatttctggt ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac
     6361 ctttataaaa cctgtattca agaaaataga ggagaaaaag gaggaagaaa ataaacctac
     6421 ttttgatgta tcgaaaaaga aagataaccc acaagtaaac catagtcaat taaatgaaag
     6481 tcacagaaaa gaggatttac aaagagaaga gcattcacaa aaatctgatt caactaagga
     6541 tgttacagct acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc
     6601 taataagttg ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat
     6661 aatgtttata gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagataaaag
     6721 ctatagaaaa aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt
     6781 gaaaatagcc atttataact caattattta gtttacttta ctttactagt gatactattt
     6841 ggagttatta atggacttag tttatataac taatgaattg attgaaaggg ttagtattga
     6901 caatattggt catattgact agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6961 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagt
//
LOCUS       DBDESG010000079         6811 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.079, whole genome shotgun sequence.
ACCESSION   DBDESG010000079 DBDESG010000000
VERSION     DBDESG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.079"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..410)
                     /locus_tag="ACKB38_002016"
                     /pseudo
     CDS             complement(1..410)
                     /locus_tag="ACKB38_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            608..2374
                     /gene="patB"
                     /locus_tag="ACKB38_002017"
     CDS             608..2374
                     /gene="patB"
                     /locus_tag="ACKB38_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGL7980720.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIVFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2513..3202
                     /locus_tag="ACKB38_002018"
     CDS             2513..3202
                     /locus_tag="ACKB38_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGL7980721.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3206..3385
                     /locus_tag="ACKB38_002019"
     CDS             3206..3385
                     /locus_tag="ACKB38_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980722.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3372..4721
                     /locus_tag="ACKB38_002020"
     CDS             3372..4721
                     /locus_tag="ACKB38_002020"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGL7980723.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     SIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4845..6305
                     /gene="gltX"
                     /locus_tag="ACKB38_002021"
     CDS             4845..6305
                     /gene="gltX"
                     /locus_tag="ACKB38_002021"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGL7980724.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIILLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6319..6753
                     /locus_tag="ACKB38_002022"
     CDS             6319..6753
                     /locus_tag="ACKB38_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGL7980725.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         2135 a         1304 c         1468 g         1904 t
ORIGIN      
        1 aggatataaa acagggttcg gataagtttt tttgcaaggt ggatgatggc tacattgtaa
       61 tgttttcctt gttctaattt agtcttaaga taggccttaa aagcaggcga aaagcgaggg
      121 catgctttgg cagcttgtat gagtacctac cgcagatgag gggaactccg tttgaccatt
      181 cttcctgcta aatcaatctg atctgactga taaatagaag aatccagtcc agcgaaagct
      241 tgtaattgag caggattatc aaaggcatga atatttcgaa tctcagctaa aatgaccgcc
      301 cctaaacgat ccccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag
      361 tcattgacac atgtttccgc cttgtcaatg agcctcttgt aatgtttgat gttttcatta
      421 cacgagataa aacgtctatg cgttatcaaa ctcattacca attaaaacaa aaagctgtgg
      481 ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg aactaatcca cagcggctta
      541 ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata atataaggag
      601 gagtaaaatg aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt
      661 tgtagttgtc atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt
      721 ttctggacaa gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc
      781 agaacttgta tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt
      841 tctatttatc tctagtgtaa tatacatgtg tctcatgacg cgcgtgattg cagaatcgac
      901 caacgagatg cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga
      961 ccgtcgacaa gatggcgata tcctgtctca ttttaccagt gatttggata atatcctcca
     1021 agcctttaac gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat
     1081 tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt
     1141 ggctttcctt atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca
     1201 gaaagaggta gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt
     1261 gattgtgcaa ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt
     1321 gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg
     1381 gatgagcctg attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga
     1441 taagtctatt gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca
     1501 gcagtactac cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt
     1561 tactggagct gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa
     1621 ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg ttttttcata
     1681 cttgcctgat aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac
     1741 agcagttgtt gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt
     1801 ttatgatgtt gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt
     1861 agatagtctt agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac
     1921 gattagagac aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc
     1981 agcaaaagca acccacattc acgactatat cgaaagtttg cctgataagt acgatactct
     2041 tattgatgat gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg
     2101 aaccctgatg acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac
     2161 ggtgacagaa agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt
     2221 cgtcattgcc caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga
     2281 tggagaagtc attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc
     2341 agaactctat cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc
     2401 tttttcttgt ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt
     2461 cattttgagt gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag
     2521 aacggttgta ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca
     2581 ttcatctgta tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt
     2641 cattcctgtt ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat
     2701 tttgacagga ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag
     2761 cgatgattac aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg
     2821 tcagaataaa ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg
     2881 tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca
     2941 ctctattgag acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt
     3001 caactcagtc catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat
     3061 tgattcaaga gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg
     3121 tttacagtgg catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga
     3181 gtatttattg aatgaactgt aacgattgga acatctggtc gttctttctt ttattttttc
     3241 aaaattttta gaatgtggca ttttacgcaa acgtttgaat tctgataaaa attggagaaa
     3301 ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt
     3361 ggaggaagaa catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg
     3421 caccacatga agtggaatac atgcaatcac aagtaacagc agcagatgaa ttaatccgta
     3481 aaggaactgg tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc
     3541 gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt
     3601 tggttgtgat cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga
     3661 atcaccattt tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg
     3721 caggaaactc aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag
     3781 acttctcagt aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc
     3841 gtgtctttaa agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct
     3901 atgcaacaac tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg
     3961 gaacatttgt tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt
     4021 tgctttcaat cgcagcatca ggagctgaca taaaagctct tatggaaggt gcgaatgcag
     4081 ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg
     4141 ttcgtaacat cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat
     4201 cacttcaata cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc
     4261 aaaaaggtat ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat
     4321 ttatccaaga aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta
     4381 aaaacgtgct tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa
     4441 aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg
     4501 gtgatgtacc aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca
     4561 ctatctactt cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat
     4621 ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag
     4681 gatttgaaga attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt
     4741 gcccactctt tttactctct ttatccatag aaattggact cagccaagac ttgtgatata
     4801 atatagaaag caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc
     4861 gcgtacgtta cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat
     4921 tgtttaatta cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata
     4981 ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt
     5041 taggcatgga ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt
     5101 tggacttgta tcaaaaatat attgaccaac tattagctga aggaaaagcc tataaatctt
     5161 acgttacaga agaagagttg gcagctgaac gcgaacgcca agaagtagct ggcgaaacac
     5221 cacgctacat caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag
     5281 aacgtgaagc agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct
     5341 acaagtggca tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg
     5401 actgggttat ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gttatcgatg
     5461 accacgatat gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa
     5521 aacagcttat ggtctatgaa gctcttggtt gggaagctcc agagttcggt cacatgacct
     5581 tgattatcaa ctctgaaact ggtaagaagt tatctaaacg tgatactaat acacttcaat
     5641 ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc agtctttaac tttattgctc
     5701 ttcttggttg gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ttcattaaac
     5761 tttttgatga aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact
     5821 ggatgagcaa tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac
     5881 cattcttaga ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata
     5941 aaccacaaat gaaatcagta gatgagatta tcctattgac agatcttttc ttctcagatt
     6001 tcccagaatt gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc
     6061 ttgaagcatt caaagcaaaa cttgaagcga tgacagatga tgaatttgtg acagaaaata
     6121 tcttcccaca aattaaagca gtccaaaaag aaacaggtat taaagggaaa aatcttttca
     6181 tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt
     6241 tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta
     6301 aataaagagg atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta
     6361 tacaatccac atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa
     6421 gcaggagatg gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat
     6481 ctctgcgcag aacgggcggc cctcttcaat atgtaccaat tttcaggaca aactaaggtt
     6541 aagaaagtat tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc
     6601 ggtgcttgta gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg
     6661 atggactata atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga
     6721 aaagaacgtg cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt
     6781 tactataagt tgaaaaaata aaaaaaaatt a
//
LOCUS       DBDESG010000080         6695 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.080, whole genome shotgun sequence.
ACCESSION   DBDESG010000080 DBDESG010000000
VERSION     DBDESG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.080"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..921)
                     /locus_tag="ACKB38_002023"
     CDS             complement(<1..921)
                     /locus_tag="ACKB38_002023"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGL7980726.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRTLRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGA"
     gene            1151..1840
                     /gene="coaB"
                     /locus_tag="ACKB38_002024"
     CDS             1151..1840
                     /gene="coaB"
                     /locus_tag="ACKB38_002024"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGL7980727.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGYEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            1852..2403
                     /gene="coaC"
                     /locus_tag="ACKB38_002025"
     CDS             1852..2403
                     /gene="coaC"
                     /locus_tag="ACKB38_002025"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGL7980728.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            2387..2950
                     /locus_tag="ACKB38_002026"
     CDS             2387..2950
                     /locus_tag="ACKB38_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGL7980729.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            3232..3759
                     /locus_tag="ACKB38_002027"
     CDS             3232..3759
                     /locus_tag="ACKB38_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGL7980730.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWIFNFVLAVVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            3771..4286
                     /locus_tag="ACKB38_002028"
     CDS             3771..4286
                     /locus_tag="ACKB38_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGL7980731.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQRLAEHFHVTRQVIVQDIAILR
                     ADGSPILSTNRGYIYKDANANTYHHKLFKVKHEVEEIGRELLAIVDNGGRVQNILIDH
                     PVYGEIETLLKLTCRRDVQHFLEQVERSDFKPISELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(4283..4750)
                     /locus_tag="ACKB38_002029"
     CDS             complement(4283..4750)
                     /locus_tag="ACKB38_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGL7980732.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(4819..5307)
                     /locus_tag="ACKB38_002030"
     CDS             complement(4819..5307)
                     /locus_tag="ACKB38_002030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980733.1"
                     /translation="MTVIIKSMETPEEIEGKSFVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGVKIVGFISYGNCRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKAALTDLNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(5273..5836)
                     /locus_tag="ACKB38_002031"
     CDS             complement(5273..5836)
                     /locus_tag="ACKB38_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGL7980734.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(5846..>6695)
                     /locus_tag="ACKB38_002032"
     CDS             complement(5846..>6695)
                     /locus_tag="ACKB38_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HGL7980735.1"
                     /translation="PLRREEFESHVHQIVYVSATPGDYENEQTETVIEQIIRPTGLLD
                     PEVEVRPTMGQIDDLLGEINARVEKNERTFITTLTKKMAEDLTDYFKEMGIKVKYMHS
                     DIKTLERTEIIRDLRLGVFDVLVGINLLREGIDVPEVSLVAILDADKEGFLRNERGLI
                     QTIGRAARNSEGHVIMYADTVTQSMQRAIDETARRRKIQMAYNEEHGIVPQTIKKEIR
                     DLIAVTKAVAKEEDKEVDINSLNKQERKELVKKLEKQMQEAVEVLDFELAAQIRDMML
                     EVKALD"
BASE COUNT         1974 a         1487 c         1160 g         2074 t
ORIGIN      
        1 agcaccaagg tccgcaccaa aaccagcttc agtaacagtg taatcagcca agtgaagggc
       61 tgttgtcgtc gccaaaacag agttacagcc atgagcgata ttggcaaatg gaccaccgtg
      121 tacaaaggca ggtgtaccgt aaattgtctg aaccaagttt ggcttaatag catccttcaa
      181 aatcaaagcc aaggcaccct caacctgcaa atcacctaca gaaacaggcg tacggtcata
      241 gcgataacca ataacgatat tcgccaaacg acgtttcaag tcctcgatgt ccgttgccaa
      301 gcaaagaatt gccatgattt ctgaagcaac tgtaatatca aaaccatcct cacgtggaat
      361 accgtttaga ggaccaccaa gaccaacagt cacatggcgg agcgtacggt cgttcaagtc
      421 cacaacgcgt ttccagagga tacgacgttg atcaattccc agctcattcc cttggtgcaa
      481 gtggttgtca atcaaggcag aaagggcatt gttggcagtt gtaatagcat gcatatctcc
      541 agtaaagtgg aggttgatgt cttccattgg cagaacttgt gcatacccac caccagcagc
      601 accacccttg atccccatga ctggaccaag agacggttcg cggatagcaa tcatggtttt
      661 cttgccaatc ttgttcaagg catccgcaag accaatggta agcgtcgact ttccttcacc
      721 tgcaggtgtt gggttgatgg cagtaaccaa gatcaattta ccgactggat tgctctcaac
      781 tgcacgaatt ttatcaaagc tgagtttagc cttgtacttt ccgtacaact ccaaatcgtc
      841 ataagaaata ccaagtttct ctacaacatc aacaattggc ttcaactcaa tactctgtgc
      901 gatttcaata tctgttttca ttcaaaattc ctctaacctc ttatatgata attcattata
      961 tcacaaaaca agatttttaa catcctaaaa ctctctaaac gttcgtaaat atctctgttt
     1021 ttaagacttt tagagtcctt tcttaaattt tatatggctt tatagtttga aactataata
     1081 aatcttcgtt tttaccaaaa atttatcact ttcattttac ttaccgctta tttttgtgta
     1141 caatagtgct atgaaaattt tagttacatc gggcggtacc agtgaagcta tcgatagcgt
     1201 ccgctctatc actaaccatt ctacaggtca cttggggaaa attatcacag agactttgct
     1261 ttctgcaggg tatgaagttt gtttaattac gacaaaacga gctctgaagc cagagcctca
     1321 tcctaaccta agtattcgag aaattaccaa taccaaggac cttctaatag aaatgcaaga
     1381 acgtgttcag gattatcagg tcttgatcca ctcaatggct gtttctgact acactcctgt
     1441 ttatatgaca gggcttgagg aagttcaggc tagctccaat ctaaaagaat ttttaagcaa
     1501 gcaaaatcat caggccaaga tttcttcaac tgatgaggtt caggttttgt tccttaaaaa
     1561 gacacccaaa atcatatccc tagtcaagga atggaatcct actattcatc tgattggttt
     1621 caaactgctg gttgatgtta ccgaagatca tctggttgac attgcacgaa aaagtcttat
     1681 caagaatcaa gcagatttaa tcatcgcgaa tgacctgact caaatttcag cagatcagca
     1741 ccgagctata tttgttgaga aaaatcagct tcaaacagtc cagactaaag aagaaattgc
     1801 agaactcctc cttgaaaaaa ttcaagccta tcattcttag aaaggaaaac tatggcaaac
     1861 attctcttgg ctgtaacggg ttcaatcgcc tcttataagt cggcagattt agtcagttct
     1921 ctaaaaaaac aaggccatca agtcactgtc ttaatgactc aggctgctac agagtttatc
     1981 caacctttga cactacaggt actctcacag aatcctgtcc acttggatgt catgaaggaa
     2041 ccctatcctg atcaggtcaa tcatatcgaa cttggaaaaa aagcagattt atttatcgtg
     2101 gtacctgcaa ctgctaacac tattgcaaaa ctagctcacg gatttgcgga caacatggta
     2161 accagtacag ctctagccct accaagtcat attcccaaac taatagctcc tgctatgaat
     2221 acaaaaatgt atgaccatcc agtaactcag aataatctga aaacattaga aacctacggc
     2281 tatcagctga ttgctcctaa ggaatcccta ctagcttgtg gagaccacgg acgaggagct
     2341 ttagctgacc tcacaattat tttagaaaga ataaaggaaa ctatcgatga aaaaacgctc
     2401 taatattgca cccattgcta tcttttttgc taccatgctc gtgatacact ttctgagctc
     2461 acttatcttt aacctttttc catttccaat caaaccgacc attgttcata ttcctgtcat
     2521 tattgccagc attatttatg gtccacgagt tggggttaca cttggatttt tgatgggatt
     2581 acttagcttg acggttaaca cgattacgat tctaccgaca agctacctct tctctccctt
     2641 cgtaccaaac ggaaacatct actcagctat cattgccatc gtcccacgta ttttgattgg
     2701 tttaactcct tacttagtct ataaactgat gaaaaacaag actggtctga ttttagctgg
     2761 agcccttggt tccttgacaa atactatctt tgtccttgga ggaatcttct tcctatttgg
     2821 aaatgtttat aatggaaata tccaacttct tctggcaacc gttatctcaa caaattcaat
     2881 tgctgaattg gtcatttctg caattctaac cctagccatt gttccacgac tacaaacctt
     2941 gaaaaaataa aaacaatctc cgctttcaaa cctgaaggtg gagattttgc tctatacagt
     3001 ttctttttaa tgaaatcttt actatagtaa aacgaaataa gaacaggaca aatcgatcag
     3061 gacagccaaa tcgatttcta acaatgtttt agaagtagag gtggactatt ctagtttcaa
     3121 tctactataa cttttaacta aaaaatgatc tataaaccca agtaaattct tgctttattg
     3181 tctggtttat tgtaccatac tagtgtatat gcagttaaaa aggagattct tgtgaataca
     3241 cggaaaaaga cacaatttat gacaatgaca gcccttttaa cggctattgc gattttgatt
     3301 ccaattgtta tgcctttcaa gattgtcatt ccacctgctt cctatacttt ggggagccac
     3361 atcgctattt ttatagccat gttcttgtcg cccttgatgg cagtttttgt catcctagcc
     3421 tctagttttg gatttttgat ggctggctat cccatggtta tcgtttttcg ggctttttcc
     3481 catatatctt ttggtgcttt aggagctctt tacctacaaa aattccccga taccctagat
     3541 aaaccaaaat cttcctggat tttcaacttt gttttggctg ttgttcatgc ccttgctgaa
     3601 gtattggcct gtgtcgtttt ttacgcaact tctggtacca atgtagaaaa tatgttttat
     3661 gttctatttg tactagttgg atttggtaca attatccata gtatggtaga ctatacatta
     3721 gcactagctg tctataaagt gcttcgaaaa cgccgttaaa aggaagaact atgacaaaag
     3781 atcgcaaaca agccctgctc caactcttga aagaagctcc taaagccctc aatggccaaa
     3841 gattagctga gcactttcat gtcactcgac aagttattgt tcaagatatt gcaattttaa
     3901 gagctgatgg atctccaatt ctatctacca atcgtggtta tatctataaa gatgctaatg
     3961 caaatactta tcatcacaaa ttatttaagg tcaagcatga agttgaagaa attggacgag
     4021 aattacttgc tatcgtagat aatggtggtc gcgttcaaaa tatcttgatt gatcatcctg
     4081 tttatggaga gattgaaacc ttactaaaac tgacctgtcg ccgcgacgtg caacattttc
     4141 tagaacaagt tgaacgttct gactttaagc caatttctga gttaacagat ggtattcatt
     4201 atcacctagt tgaagccgag acacaacaag acctccacta tatcgaggag gccttggatc
     4261 agctaggtta tttagtaaaa gactagaaga ttttcttttc ccaatcgtct tctgtacggc
     4321 gagggtagaa aaactctgac ttgtcgggag cttccatcat ttccatcaat ggtagcatat
     4381 cataggccag atttaagttt ggaatctggt ctttttgcac ccaagaaact tctccctctt
     4441 ctgaagattg aagggtacca gagaactcag tcgccttata acaaatgaca atatagcgcc
     4501 cacctgtatc tagtggccaa tttttaatgc cgacaagttg aggattttgg atagtcaacc
     4561 ctgtttcttc gtagatttca cgaatgacag actccgcaaa agcctcatca ttttctacat
     4621 gacctccagg aaaggcataa ccagaccagc gattgttttc aggggcgcga tactgcatca
     4681 ccacgcgctg agtttcgagg tcttcaatca gacagatatt tgttaaaatt gttaattggg
     4741 aacgggacat aaatttactc gctttctaat ttattaaatt ttcagacttg gtaccatatt
     4801 agcttttact tttgagaatt atttagaata gaataccatc cgtttttcct ttataggctt
     4861 tccaagttca agtatttttt cttgtccatc aaaagtaaaa cccatttttt gatagaaagc
     4921 aatggcgcgc ttgttatctt tcaataccca taagaaaatt tcagaaaaat gattaagatc
     4981 agtcaaagct gctttcacta acttttgtgc gattcctttt ccataatagt cttttaaaac
     5041 atataaagca ataatttcac cagcttgaat agtctcatca cgacagttgc cataacttat
     5101 aaaaccaact atcttcacac catccatcgc aatcaatgta ttttctggat acttttgact
     5161 aaagagtcga catctttcta atgtcattgt ctcctgaaat tccgcaggca aaaggtcatc
     5221 ataagcctct ctccacgttt gccagtgaac gaaggattta ccttctatct cttcaggagt
     5281 ttccattgat ttgataataa ccgtcattta ttttctccca gtcttctctc aaaataccat
     5341 atttaatact gtcaaaatat ttaccttgat aataacgaac ttttggaata tgagcttctt
     5401 ttttcattct taatttttca gcaagtttca tcataccaat atttcctgac caagttgtca
     5461 aacccagatg ctccaactcc aagtaatcct gaaacgtcct atctatccac tgcaacatag
     5521 caactttccc aataccagtg ttccagaatt ttttatcata aataccaatt cccaattcca
     5581 tccatcttgt ttctttacat acccaataac gcgaaacagt cccaactagt ttatcatcaa
     5641 caaaaatacc aaggcgattt gagttgctta aaatggattc tgatttttgt agttcgaatt
     5701 ctttaaaatt tgaaaaatac tgataatcat cataataggg agcatcatac tgtttccaaa
     5761 ctggattaag ctgtgaataa gccatttccc ataaagacat caaatcttct ttctttaact
     5821 ttcttaaata aatcatacta ttcccctaat ccaaggcctt gacttccagc atcatatcac
     5881 gaatctgagc tgctagttca aagtcaagca cttcaactgc ttcttgcatt tgtttctcaa
     5941 gctttttgac tagttctttg cgctcttgtt tgttgaggct attgatatcg acttccttgt
     6001 cttcttcctt agcaactgcc ttggtcacag caatcaagtc acggatttct ttcttgatgg
     6061 tttgtggaac gataccatgt tcttcattat aggccatctg gattttgcga cggcgggcag
     6121 tttcatcgat agcacgttgc atagactggg taaccgtgtc cgcatacatg ataacatgac
     6181 cttcgctatt acgtgcagca cgtccaatgg tctggatgag tccacgttcg ttgcgaagga
     6241 aaccttcctt gtcagcatcg agaatagcta cgaggctcac ttcaggaacg tcaattcctt
     6301 cacggagcag gttaattccg accaagacat caaagacacc caagcgcagg tcacggataa
     6361 tctccgtccg ttccaaggtc ttgatatccg agtgcatgta cttgaccttg atacccattt
     6421 ccttgaagta gtcggtcaaa tcctctgcca ttttcttggt caaagttgtg ataaaggtac
     6481 gctcattttt ttcaacgcgg gcattgattt cacccaagag gtcatcaatc tgtcccatag
     6541 tcggacggac ttccacctct ggatccaaga gtcccgttgg acgaatgatt tgctcaatca
     6601 ctgtctcggt ctgttcattt tcatagtcac caggtgtcgc tgaaacgtaa acaatctgat
     6661 gaacgtgact ctcaaactcc tcccgacgga gagga
//
LOCUS       DBDESG010000081         6547 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.081, whole genome shotgun sequence.
ACCESSION   DBDESG010000081 DBDESG010000000
VERSION     DBDESG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.081"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..345
                     /locus_tag="ACKB38_002033"
     CDS             <1..345
                     /locus_tag="ACKB38_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980736.1"
                     /translation="LVPELKDYYDLYQLLLFHLQEKNTDQFWGLIQDTLPHLNRTFKT
                     TLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGFRNFENFKKRIFIALNI
                     KKERTKFVLSQA"
     gene            complement(483..2807)
                     /gene="pflB"
                     /locus_tag="ACKB38_002034"
     CDS             complement(483..2807)
                     /gene="pflB"
                     /locus_tag="ACKB38_002034"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGL7980737.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            3155..4216
                     /gene="dinB"
                     /locus_tag="ACKB38_002035"
     CDS             3155..4216
                     /gene="dinB"
                     /locus_tag="ACKB38_002035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGL7980738.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(4363..5208)
                     /locus_tag="ACKB38_002036"
     CDS             complement(4363..5208)
                     /locus_tag="ACKB38_002036"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGL7980739.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(5239..5376)
                     /locus_tag="ACKB38_002037"
     CDS             complement(5239..5376)
                     /locus_tag="ACKB38_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980740.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            5463..6547
                     /locus_tag="ACKB38_002038"
                     /pseudo
     CDS             5463..6547
                     /locus_tag="ACKB38_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826497.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         2012 a         1346 c         1289 g         1900 t
ORIGIN      
        1 ctagtacctg aacttaaaga ttactatgac ctgtatcaac tactcctatt tcatctacaa
       61 gagaagaata ctgaccagtt ttggggctta attcaagaca cgttacctca tctcaaccgc
      121 acctttaaaa ccactttgag cacatttatt tgctataaaa actacatcac taacgccatt
      181 gaattgcctt attctaacgc taaacttgag gccactaaca aactcatcaa agacatcaag
      241 cgcaatgcct ttggttttcg gaactttgaa aacttcaaaa aacggatttt tatcgccctg
      301 aacatcaaaa aagaaaggac gaaatttgtc ctttctcaag cttagctttt tttcaaccca
      361 ctacagttga caaagagccc aaaaacattg catattgcgc cgaaggaatt gctcatacta
      421 tcccaagcac aaaaccctca gtcgtttgac cgagggttcc tttttattat tcaagaactt
      481 gattagctca atgcatccaa ggcgtcatcc attgaaagaa cttcgtggaa gacacgttgt
      541 gtcaattcag ttttttgttc tggagtgagg tatttagtgt ttacacagta tccagagata
      601 cgtacgataa cgtcttcgcc tgacatgatt ttttcgtaaa catcgttcaa gtccataacg
      661 ttcaagttaa cgtgttgtcc accgttttcg aagtaaccat caagaattgt taccaagtta
      721 tcaacttgtt catcacgagt cttaccaaga gcgcgaggtg atacttgtgt agtcaatgag
      781 ataccgtcag ctgcataact aaagtcaagg ctagaaagtg agttcaagtt ttgcaaccaa
      841 ccacctttag ctttgttaga tgggttagca cctggtgaga agaattcaag tttagacaag
      901 ttcacagaac catcttcgtt gaggtataca cctttgtgaa ctggtgagtt accagtttgt
      961 ttagagtaag caacgttaga tgtgattgtc aaaagtgata ctgtagcttc tgcgtctttg
     1021 tatagtttgt ggctacgtag acgagttgtg taagcttcga tcaaccattc tgccaattcg
     1081 tttgaacgtg ggtcatcttc accccagcgt gggtagtcac cgattgtttc gtaatcgtag
     1141 atgtagccat cttcgtcacg gattggttta actgtagcgt atttgatagc tgacaatgta
     1201 tcaacagtgt tagcaaatcc acagataccg aatcccatgt tggcacgttg tttagttggc
     1261 aagaaggcca tttgaacagc ttcgtagttg tacctatcag tcatgtagtg gatgatgttc
     1321 aaggcatcta cgtaagtgtc agtcaaccag tcaagagatt tttcaaagtt cgctttaact
     1381 gattcaaatt caagaacttc gtcacggatt ggttcgatat caaatacttt gtagtctttg
     1441 tgaacatcgt cgtaaccacc attcaaacca gtaagaaggg ctttaagaac gtttacacga
     1501 gcaccgaagt actggatgtt gtggcgttgt tcttcatttt ctggatcaag tggagacaca
     1561 cagcatgaga tacagctcat ttcaccatat ccgtctttag ccattgttgt tacaccttcg
     1621 tattggatag aagagtgttt gtggctcatg tgcatacagt agcgacggaa gttgtatggc
     1681 aatttgtcag tccaaagaac tgtcaagttt ggttctggtg agttaccgat gttgtcaaga
     1741 gtgttcaaga aacggtagtc catcttagta acacggtgac gaccgtcgtt acccatacca
     1801 gccatagaag ttgtgataaa ggttgggtca cctgagtaca attggtcata agcttttgta
     1861 cgagcaaatt taactgtacg aagtttcata acgaaatcat caacgaattc ttggatttct
     1921 gattcagtaa atgtaccacg agcaaggtca cgttctgcaa agatgtccaa tacgattggt
     1981 acacgaccta gagatgtagc agcaccgtta atcacacggc agacagccat gaaagcaatg
     2041 ttaacccatt ggattgcttc tttcacgttc atcgctggtt tgcgaacatc aaccccgtaa
     2101 aggtcaccca ggcgaacaac ttgttgcaat gcttggtatt gaaggtttac ttcttcacga
     2161 agacggattg tttcttcatc gatttctttg attgcattcc agtcatttac tttttcttgc
     2221 atcaagtagt ctgcaccgta aagagcaaga cgtgcgtaaa caccgatgat acgtccgcgt
     2281 gagtatgcat ctggaagacc agttacagtg tgtgcgtgac gagcgcgacg aatatttgaa
     2341 gtgtaggcac ggaaaatacc gtcgttaact gttgttacat atttagtgaa gatttcgtga
     2401 acagctgggt ctggttcgta tccattttct ttcaaagtag tttcagccat acggatacca
     2461 ccttttggca tgaagttcaa tttgaagagt tcatcgtttt ggataccgaa gataacttca
     2521 ttttctttgt cgataaatcc agcagggata tcagcgatag atgttggacg agtgtccatt
     2581 gggaaacgag tttcttcgta gtgtgcttta gtttcttcta caattttctt gatgtgaagt
     2641 gaacgctctg ttggtcctgc aaggaagctt tcgtctccat cataaggtgt gtagttagct
     2701 tgtacaaatc gtgatacact tgctttttct ttccaatcta cgcctttgaa gccttcccaa
     2761 gctttgtcaa aaatatcttg tgcttcaaca actgtcttaa caaccatatt aatgtcctct
     2821 tttttctagt aacagtcatc tgttacattc ataagacaag tataccatat agtaaccgat
     2881 ttcaacaagt gaaaattccc tatttttaca ctttcttttc taaaacagtc tatattttat
     2941 ttcaaactgt attatatttt tgaaaaaata aagtcctttt ttcttttttt cagaaaaaag
     3001 ggtataataa aagaaaataa gcagtaacac tcaatggaaa tcgaaaagca aactaggaag
     3061 ctagccgcag attgctcaaa acactgtttt gaggttgcag atagagctga cgtggtttga
     3121 agagattttc gaagagtata aaaaggtgct aggcatgttg atttttcctt tgttaaatga
     3181 tttgtcaaga aaaatcatcc atattgacat ggatgccttt tttgctgcag tggaaatcag
     3241 ggataatcct aaactcagag gaaaacctgt cattattgga agcgaccctc ggcaaacagg
     3301 tggacgggga gtcgtttcta cctgtagtta tgaggcaaga gcttttggtg tccattctgc
     3361 catgagttcc aaggaagctt atgaacgttg tccccaggct gtctttatct cagggaatta
     3421 tgagaaatac aagtctgtgg gactccagat tcgagctatt tttaagcgct atacagattt
     3481 gattgaaccc atgagcattg acgaagccta tttggatgtg acagaaaata aactcggtat
     3541 caagtcagcg gtcaaaattg ctcgcctcat tcaaaaagat atctggcaag aactccatct
     3601 aactgcttcc gcaggcgttt cttacaacaa attcttagct aaaatggcga gtgattatca
     3661 aaaaccacat ggtttgacag tgattctacc tgaacaggct gaggattttc tcaaacaaat
     3721 ggatatttcc aaatttcatg gagtaggaaa aaagacagta gaacgtcttc atcaaatggg
     3781 cgtttttact ggtgctgatt tacttgaagt tcctgaggta accctaatag accgttttgg
     3841 tagactaggc tatgatctgt atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc
     3901 caatcgcatc cgtaaatcaa tcggcaagga gaaaacctac gggaagattc tccttgctga
     3961 ggaagatatc aaaaaagagc tgactcttct atcagaaaaa gtcgctctca atctacatca
     4021 acaagaaaaa gctggaaaaa ttgtcatttt gaaaatccgc tacgaggact tttcaactct
     4081 taccaaacga aaaagtcttg ctcaaaaaac acaagatgct agtcagataa gccaaatagc
     4141 cctgcaactc tatgaagaat taagcaagaa agaaagaggt gtccgcctat tggggattac
     4201 catgactgga ttttaaagct caatgaaaat caaagagcaa actaggaggc tagccgcagg
     4261 ctgctcaaaa cactgttttg aggttgcaga taaagctgac gcggtttgaa gagattttcg
     4321 aagagtataa aaccttgaag agttgcccct tcaaggtttt tcttatacaa ataaacggac
     4381 aaaactgtaa agtagcaaaa cactaccaag cacgatacgg tatttaccaa aaagggtgaa
     4441 gtcgtgtttt ttcacatagc tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc
     4501 tactcccatc gcgaccaaga gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt
     4561 cacaaatttg aaaatcttta aggcactagc tccaaacata acaggaatcc caagatagaa
     4621 ggtaaattct gtcacaactg aacgactggt tccatttaac aaaccaccga caatcgttgc
     4681 acctgaacgg ctagtccctg gtaaaagagc aagaacttgg aagagtccga tatagaaagc
     4741 ggtcgtataa ggaagcttgt ccaactctgt tacacttggc tcgatagcac gcgctttatt
     4801 gcgcttttcc aaatagatga aggcaacccc gtagataatc aacatgagag caactgaaac
     4861 catgttatgg aagtgggtat caaaccaatc atcaaattta aagacaccaa gtaaaggtaa
     4921 agtagcaatc aagaccttca accatagtct ccaagtctta cgaacttcct gtttgtcctt
     4981 ggtcggttta aaaggattga gcttgttaaa ataaatcacc ataactgcta aaatagcacc
     5041 aagctgaatc acgacattaa acatggacat aaaggcttca ttttgatttt ggtattggat
     5101 gaattcctct gctaaaatca agtgacctgt actggaaatc ggcaaccatt ccgtaattcc
     5161 ttcaacaatt ccgaagaaga tagattttaa aatttcaata agatacatag attactccct
     5221 atattatatt tagcgcagtt agctactgcc aaagcccaag tggtatactt ggaataagcc
     5281 actatggatt agttcatttc ctccaatcgc ttgacgattt ccgaaaggct ctaaccacag
     5341 cttttgtttc aattggtaat gagtttgata acgcatagac gtttgatctc gtctaaggta
     5401 acaaggcaaa aaattcgtgg ataaccaagt agacttgaaa tactatatct ggaggtactc
     5461 ccatgcttta tgtaggcatt gatatcgcta aaaataaaca cgatgttaca gccttgaatg
     5521 ttccaggaaa aactgttctt aaaccactca ctttttcaaa taataaagct ggttttgaac
     5581 tcttagatct gtctattcga cagatcaacc aagactgtct catcgctctt gaagatactg
     5641 gacattatgc ctttaatcta ctgaacttcc tgcatgaaca aggatataaa gtttacacct
     5701 acaatccttt gctcatcaag gaatttgcca aatcattatc acttcgcaag actaaaacgg
     5761 ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat cgtgaacaat
     5821 ttcaacacga taatcggcaa gtagacctaa aaatactggc tagacatatt catcgtctca
     5881 agaaaaacag tctgattgga aagtacaata cactcgttgt cttgatatca tctttcctga
     5941 gttggataaa atcgttggaa agcattcaga atatacctac caactcttga cacgctaccc
     6001 taatcctcag aaaaggattg aggcaggatt tgataagctg atagaaatta agcgattgac
     6061 cgcttctaaa attcaggata tcctctcagt cgcacctcgt tctatcggaa caacatctcc
     6121 tgctcgtgaa ttcgaaatca tcaagcatta caagaggctc attgacaagg cggaaacatg
     6181 tatcaatgac tcgatggctg agttcaactc ggtcatcacg acggttactg ggattgggga
     6241 tcgtttaggg cggtcatttt agccgagatt cgaaatattc atgcctttga taatcctgct
     6301 caattacaag ctttcgctgg actggattct tctatttatc agtcaggtca gattgattta
     6361 gctggaagaa tggtcaaacg gggttcccct catctgcggt gggcactcat acaagctgcc
     6421 aaagcatgcc ctcgcttttc acctgcttct aaggcctatc ttaagactaa gttagaataa
     6481 ggaaaacatt acaatgtagc catcatccac cttgcaaaaa aacttatccg aaccctgttt
     6541 tatatcc
//
LOCUS       DBDESG010000082         6426 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.082, whole genome shotgun sequence.
ACCESSION   DBDESG010000082 DBDESG010000000
VERSION     DBDESG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.082"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            54..1016
                     /locus_tag="ACKB38_002039"
     CDS             54..1016
                     /locus_tag="ACKB38_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGL7980741.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1193..2200
                     /gene="gap"
                     /locus_tag="ACKB38_002040"
     CDS             1193..2200
                     /gene="gap"
                     /locus_tag="ACKB38_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGL7980742.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2268..3143)
                     /locus_tag="ACKB38_002041"
     CDS             complement(2268..3143)
                     /locus_tag="ACKB38_002041"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGL7980743.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVHWKEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTF
                     TTLSNTFEKELKKNG"
     gene            3230..5425
                     /gene="pbp2a"
                     /locus_tag="ACKB38_002042"
     CDS             3230..5425
                     /gene="pbp2a"
                     /locus_tag="ACKB38_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGL7980744.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5478..5630
                     /gene="rpmG"
                     /locus_tag="ACKB38_002043"
     CDS             5478..5630
                     /gene="rpmG"
                     /locus_tag="ACKB38_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGL7980745.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5640..5816
                     /gene="secE"
                     /locus_tag="ACKB38_002044"
     CDS             5640..5816
                     /gene="secE"
                     /locus_tag="ACKB38_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGL7980746.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5871..6407
                     /gene="nusG"
                     /locus_tag="ACKB38_002045"
     CDS             5871..6407
                     /gene="nusG"
                     /locus_tag="ACKB38_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGL7980747.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         1999 a         1123 c         1400 g         1904 t
ORIGIN      
        1 tcacggatac agttgggaaa gaagctatta aatatttgga gagtcctttt gggatgaaca
       61 tgtcaaatat tactatagat gggcaagaaa tggatctgag tcaaacgatg ccttcttatg
      121 ctcaagtagt gagtctttat gatgatacaa gtatctctgt tagtaataat gagacggaca
      181 aagtgttggc tggatccctc tatactgata caaatgaaca gggattaacg attccaagca
      241 gtttactaaa aaattggaat gaacagacag gaaaaaattt gacagctaat gatcttattg
      301 gcaaatcagt ctcagccagc attgtagaaa gtgctgccga aactagtaag attgctcaat
      361 ttcaaacgaa gattgtacgt gtaatcaatg atgaagatga catggaggac agcaacagtt
      421 tcatgctgtc tcatcaaatg gaaacgattt tgaaagaggc tggatttacg aaagctgtat
      481 cttattttat cttggaactc aaagatccat cacagacaaa agtagtaaca gaagaattac
      541 agaaaaataa gaagtatact gtgctttctc aacagagagt tcttgatatt gtgattacct
      601 ttattcgtgt tattcaggga ttattgattg tgctttcatc acaagctatt gtggtagcag
      661 cggttatgat tggtatcatt atttacatca atatcatgca acgttccaag gaaataggtg
      721 tcatgaaagc agttggttat cagaatcgtg atgtcaaagg aatttttatt tacgaggcta
      781 tctggattgt aggcatcgcc ttgctgctgg catttttggt tgcacaaggg gtgggaagtt
      841 tggcgaatgc gattgtaagt cacttttacc catccatcac taaggttttt gaattaaatc
      901 ttttatctgt tttaggaact ctagttttcg ctctattact tggttatgtc tcagcctact
      961 tcccggcgcg taagattagt aaaatggatc ctatagaatc gttatgctat gaatagtaag
     1021 ggattccttg gtttacagtc ttaaaaagaa actaacatag aaaaacaatg gccatacttg
     1081 caattcaact caaatagttg tataataggt ttgttgaaag gtgatttgta gtgattcaag
     1141 ttactctttt cacaataaaa atttcatgat tttcataagg aggaaatcac taatggtagt
     1201 taaagttggt attaacggtt tcggacgtat cggtcgtctt gctttccgtc gtatccaaaa
     1261 cgtagaaggt gttgaagtta cacgcatcaa cgaccttaca gatccagtta tgcttgcaca
     1321 cttgttgaaa tacgacacaa ctcaaggtcg tttcgacggt actgttgaag ttaaagaagg
     1381 tggatttgaa gttaacggta aattcatcaa agtttctgct gaacgtgatc cagaacaaat
     1441 cgactgggct actgacggtg tagaaatcgt tcttgaagct actggtttct ttgctaagaa
     1501 agaagcagct gaaaaacacc ttaaaggtgg agctaaaaaa gttgttatca ctgctcctgg
     1561 tggaaacgac gttaaaacag ttgtattcaa cactaaccac gacgttcttg acggtactga
     1621 aacagttatc tcaggtgctt catgtactac aaactgcttg gctccaatgg ctaaagctct
     1681 tcaagacaac tttggtgttg ttgaaggatt gatgactact atccacgctt acactggtga
     1741 ccaaatgatc cttgacggac cacaccgtgg tggtgacctt cgccgtgctc gcgctggtgc
     1801 tgcaaacatc gttcctaact caactggtgc tgcaaaagct atcggtcttg taatcccaga
     1861 attgaatggt aaacttgacg gatctgcaca acgcgttcca actccaactg gatcagttac
     1921 tgaattggta gcagttcttg aaaagaacgt tactgttgat gaagtgaacg cagctatgaa
     1981 agcagcttca aacgaatcat acggttacac agaagatcca atcgtatctt cagatatcgt
     2041 aggtatgtct tacggttcat tgtttgacgc aactcaaact aaagttcttg acgttgacgg
     2101 taaacaattg gttaaagttg tatcatggta cgacaacgaa atgtcataca ctgcacaact
     2161 tgttcgtact cttgaatact tcgcaaaaat tgctaaataa ttcttgagtt gatagaaagc
     2221 aaggctttgt ggtcttgctt ttttatatgg aaaaatggat gacacgatca tccattcttt
     2281 tttaattctt tttcaaatgt atttgaaagg gtagtgaaag ttagcttctc taaagtaagt
     2341 gggtgggtaa aggaaagtcg gaaggcatga agcataagcc ggcttgtctt tgatttacta
     2401 ttatagagag ggtctcccag gataggaaga ttatgatgcg aaaggtgcac acgaatctga
     2461 tgggttcgcc ctgtctttag cttgcaatga gccaaggaag tcttgtttga gaattgcttt
     2521 aatctgctta catgcgtttc agcatattgc ccattttttg catcaactat tctttttcta
     2581 cgatcatggc gatcacgtcc aattttgtct ctgaaaacaa gttcttttct gttgatattt
     2641 ccatcaacta gagcccaata ttctctagaa atctcttttt tctccaataa gcgattgaga
     2701 atgggcagga taaaaggatt tttggcaaag agaactaagc cactggtttc catgtccaga
     2761 cgatgaacga catagcaggt ttggccaaca taggtactga catggttaag aagggcaatt
     2821 tcgtttggtt gattaccatg cgttttcatc ccctctggtt tgtttacaat aatcaagtgt
     2881 tgatcttgat aaacttcctg cactaagtct gggttgcccc aagggatcgt cttttgggaa
     2941 taatcttcct cgtcaaaagt caactggcaa acatctccag gatttacgat ttccttccag
     3001 tggacttctt cttgatttat caaaatatgt ttcttgattc tcaaaaaatg acggattttt
     3061 ctagggatga ggagttgttc ctcaagtaat tgctttactg tcatttgagg tagagaggcg
     3121 ggtaatgtaa atgtgaattg catacagata ttgtaacaaa aaaagcccta tttggatagg
     3181 aaatagctaa attcttgtct tcctatgatg aagatgataa aataaacgca tgaaattaga
     3241 taaattattt gagaaatttc tttctctttt taaaaaagaa acaagtgaac tagaggactc
     3301 tgattctact atcttacgtc gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc
     3361 gattcgaaaa ttctggcgtc gttatcatct aacaaagatt atccttatac taggtttgag
     3421 tgcaggcttg ctagttggaa tctatttgtt tgctgtagcc aagtcgacca atgtcaatga
     3481 tttgcaaaat gccttgaaaa ctcggactct tatttttgac cgtgaagaaa aagaggctgg
     3541 tgccttgtct ggtcaaaagg gaacctatgt tgagctgact gacatcagta aaaacttgca
     3601 gaatgctgtt attgcgacag aagaccgttc tttctataaa aatgacggga ttaactatgg
     3661 ccgttttttc ttggctattg tcactgctgg acgttcaggt ggtggctcta ccattaccca
     3721 acagctggct aaaaacgcct atttatcgca ggatcaaact gttgagagaa aagcgaaaga
     3781 atttttcctt gccttagaat taagcaaaaa atatagtaag gagcaaattc taaccatgta
     3841 ccttaacaac gcttattttg gaaatggtgt gtggggtgta gaagatgcga gtaagaaata
     3901 ctttggagtt tctgcatcag aagtgagtct ggatcaagct gcgactctgg cagggatgct
     3961 caaggggccg gaactgtata atcccttgaa ttccgtagaa gattctacta atcggcgcga
     4021 tactgtcttg cagaatatgg ttgcagcagg atatattgat aaaaaccaag aaaccaaagc
     4081 tgctgaagtt gatatgactt cgcaattgca cgataagtat gaaggaaaaa tctcagatta
     4141 ccgttacccc tcttattttg atgcggtggt taatgaagct gtttccaagt ataatctaac
     4201 agaggaagag attgtcaata atggctaccg catttacaca gagctggacc aaaactacca
     4261 agcaaatatg cagattgttt atgaaaacac atcgctattt ccgagggcag aggatggaac
     4321 gtttgctcaa tcaggaagtg tagccctcga accgaaaaca gggggagttc gtggagttgt
     4381 cggtcaagtt gctgacaatg ataaaactgg attccggaat ttcaactatg caacccaatc
     4441 aaagcgtagt cctggttcta caattaagcc tttagttgtt tatacgccag cagttgaagc
     4501 aagctgggct ttgaataagc agttggataa ccataccatg cagtatgaca gctataaggt
     4561 tgataactat gcagggatca aaacgagtcg agaagttcct atgtatcaag ccttggcaga
     4621 atcgcttaat ctacctgctg ttgccactgt taatgatttg ggtgtcgaca aggcttttga
     4681 ggcaggcgaa aaattcggac tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt
     4741 gggaagcggt gttgaaacca accctcttca aatggctcaa gcatacgccg cctttgcaaa
     4801 tgaaggttta atgcctgaag ctcattttat tagtagaatt gaaaatgcta gtggacaagt
     4861 tattgcgagt cataaaaatt cacaaaaacg ggtgattgat aagtctgtag ctgacaagat
     4921 gaccagtatg atgttgggga ctttcaccaa cggtaccggt attagttcat cgcctgcaga
     4981 ctatgtcatg gcagggaaaa ctggaacaac tgaagcagtt ttcaatccgg agtacacaag
     5041 tgaccagtgg gtaattggtt atactccgga tgtagtgatt agccactggc ttggctttcc
     5101 gaccactgat gaaaaccact atctagctgg ctctacttca aacggtgcag ctcatgtctt
     5161 tagaaacatt gccaatacca ttttacctta tacgccagga agtaccttta cggttgaaaa
     5221 tgcttataag caaaatggaa ttgcaccagc caatacaaaa agacaagtac aaaccaatga
     5281 taatagccag acagatgata atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga
     5341 tgaggctagc cgggctatct cagatgcgaa gattaaggaa aaggctcaaa caatatggga
     5401 ttcgatagtc aatctatttc gctaagatgc ttgtcaaagc ctagctttct tgttataatg
     5461 gataagatgg aggcgttatg gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt
     5521 cgagaaacta ttcaatcaag atcagcggaa acctcaagcc tacacgacta gaagtaaata
     5581 aattttgtaa gcattgtggc aagtacacta cacacagaga aacgagatag gagagagcga
     5641 tgcgttttat tggagatatt tttagacttc ttaaagacac aacatggcca actcgcaagg
     5701 aaagctggag agattttcgt tctatcatgg aatacacagc tttctttgta gtaattattt
     5761 acatttttga ccagttgatt gtttcaggtt tgattcgatt tattaacatt ttttagaaga
     5821 ttagtggagt taattacact agaaatcttc tatttatgaa aggaaatatc atggatagtt
     5881 ttgataaagg gtggtttgtt ttacaaactt attctggtta tgaaaataag gtgaaagaaa
     5941 atctattaca acgtgcacaa acctacaata tgttggataa tattctacgc gttgaaattc
     6001 caacacaaac agtgcaagtt gaaaaaaatg gaaagagaaa agaagtagaa gaaaatcgct
     6061 ttccaggtta tgttcttgta gaaatggtca tgacagatga agcttggttt gttgttcgaa
     6121 acacaccaaa tgttacagga tttgtcggat cacacgggaa cagatcaaaa ccaactccat
     6181 tattggaaca agaaattcgc gacattttgg tatctatggg acaaactgtt caagaatttg
     6241 atttcgatgt tgagattggt caaactgtac gtattattga tggtgctttt gcagactaca
     6301 ctggtaagat tacagaaatt gataataaca aagtgaaaat gattatctct atgtttggta
     6361 atgacacagt tgcagaagta aatctaaacc aaatcgcaga attataaccc tgagagaggc
     6421 tggagc
//
LOCUS       DBDESG010000083         5995 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.083, whole genome shotgun sequence.
ACCESSION   DBDESG010000083 DBDESG010000000
VERSION     DBDESG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.083"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..210)
                     /locus_tag="ACKB38_002046"
     CDS             complement(<1..210)
                     /locus_tag="ACKB38_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980748.1"
                     /translation="MNTIERTRRLIKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDET"
     gene            complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="ACKB38_002047"
     CDS             complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="ACKB38_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGL7980749.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1709..2308)
                     /locus_tag="ACKB38_002048"
     CDS             complement(1709..2308)
                     /locus_tag="ACKB38_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGL7980750.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2309..3406)
                     /locus_tag="ACKB38_002049"
     CDS             complement(2309..3406)
                     /locus_tag="ACKB38_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGL7980751.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(3406..4143)
                     /locus_tag="ACKB38_002050"
     CDS             complement(3406..4143)
                     /locus_tag="ACKB38_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980752.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(4145..5029)
                     /locus_tag="ACKB38_002051"
     CDS             complement(4145..5029)
                     /locus_tag="ACKB38_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGL7980753.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(5016..5210)
                     /locus_tag="ACKB38_002052"
     CDS             complement(5016..5210)
                     /locus_tag="ACKB38_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980754.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(5207..5413)
                     /locus_tag="ACKB38_002053"
                     /pseudo
     CDS             complement(5207..5413)
                     /locus_tag="ACKB38_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1913 a         1348 c          968 g         1766 t
ORIGIN      
        1 agtttcatct acaacctcaa aaacatgttt tgagctgact tcgttagttt catctacaac
       61 ctcaaaaaca tgttttgagc tgacttcgtc agttctatct gcaacctcaa agcagtgctt
      121 tgagctgact tcgtcagttc tatctacaac ctcaaaacag tgtgttgcgc agcctttaat
      181 cagccgccta gtccgctcta tggtattcat taagtcaaca tctcttgttt aagagcacca
      241 aatcaggaaa tcttctcgat tccctgattt tttctattta cgttttcgtg ttgagctacg
      301 ttctgtcaaa ccatgaggta agagaacttc acgttcttcc aactcttcct tatgcataat
      361 cttggtcaac atacgcatac taatggcacc aaggtcataa agaggttggg caatcgttgt
      421 caagtttgga cgggtaaagc gtgagatttg tgaatcatca ctagtaataa tttcaaaatc
      481 ttctggcaca gaaacaccct tatcagccaa accgttcaag actcctgctg ccaactcatc
      541 acctgtcaca actgctgcag ttgcatttga tgaaatcaaa cgctctgcta aggcgtaacc
      601 atcatcatag ctatatttag attcaaatac caaaccctca ctataagtga ttcctgcttt
      661 tttcaaggtt tccttgtagc caactaaacg aaccttacca ttgatgtcat ccactagcgg
      721 accgctaacg aaagcaatac gctcattttc tttagcaagg taactcactg catcaattgt
      781 tgcttgctta tagtcaatat tgacacttgg caactggtgc tcaacatcga cagttcctgc
      841 gagaacaatc ggagtacgtg aacgcgaaaa ttctgagcga attttatctg tcaagtgata
      901 ccccatatag ataatgccat ctacctgctt tgaaaagagg gtattgacaa cagaaacttc
      961 tttctcgtta tcttcatcgc tattagctag gacaatattg tacttgtaca tttctgcaat
     1021 atcatcaatc cccttagcca aactcgaaaa ataaccattg gtaatatttg gaatcacgac
     1081 accgacagtg gttgtctttt tacttgcaag accacgcgca actgcatttg gacgataatc
     1141 caaacgatca attacctcta gcactttttt acgggtattc tcttttacat ttttattgcc
     1201 attgaccaca cggctgaccg tcgccatgga aacacctgct tcacgagcga catcataaat
     1261 ggttactgta tcatctgcat tcattccttt tcctgtcctt tctatctcac acattctttt
     1321 acaagtagag gtactgattg aagctctata tctacttaca aaagtgaaga tgtgaaaatt
     1381 tcgttttcat atttctactt attccattct atcactaatt gtaaacactt tcaagtgttt
     1441 tttgaagatt gattgaaaaa atttcataga aaacctaggt ttagctcctt gctaccacct
     1501 tagactaaac aaaaaggagg aaactaagcc ctcctaaagt tatagtaaaa tgaaataaga
     1561 acaggataaa tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
     1621 gtactattct agtttcaatc tactataggt attgttccat tcactaccgt caattttagc
     1681 acatagtctt catgaaaata ttatatcatc ataaccaacc agattctttc gcgatattag
     1741 ctgcctctgt tcgattacct gcatctagtt tcgaaagaat attggtgaca tagtttcgga
     1801 ctgttccgtt ggatagataa agtttgtctg caatttcttg gttagagaag ccctgagcaa
     1861 ttccctttaa aactgcgatt tcttgctccg ttaatggatt gggatgcatc atcaccactt
     1921 ccatcaattc aggcgaatac tccttgcgtc cttcgaggac ggtgtgcaag gtttgcatga
     1981 ggtctgcaat gtttctttct tttaatacat aagcatctac tccagccttg accgcacgtt
     2041 caaaataccc aggacgcttg aaggtcgtca ccacaaccac ctttgtttca agcttttctg
     2101 ctcgtatcca ctccaagact tcaagacctg tcttaacagg catttctacg tcaaggatgg
     2161 cgatatctac agactccttt tctaatagtt ggattgcttc ttgcccattc ttggcttgaa
     2221 agacagactc tacatccggt tgaagcatga gcaactggca catggcatct cgcaacatac
     2281 tttgatcttc tgcgactaat actttcatct actttctctc cttataaagt agtcgaacct
     2341 gcacttcagt tggatgtttc tgactgatta cacttacttc tcctgaaaat ggaaaaacac
     2401 gatttcggac tgtatggagc tcatccccgc ttatagaggc aaagccacag ccatcatctc
     2461 tcactgttag aatgagttct ttctctgtcc gttctaattt caagtagact ttagacgctt
     2521 tagcatgttt gatgatattg gtcactaatt caagcaaaat catggaagcc gttgactcca
     2581 attcctgagt taagctagac ttgtccaagt gattctcaac ttgaacctca attccagcaa
     2641 tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac ttaagatttt
     2701 ccacaatggt tcgcacttca ttcatggaat ccttgctgat ctggtgaatt tcttttaatt
     2761 ccttttccac ctgtggataa gcctccatct gaaataactg caaggctaaa tctgtcttga
     2821 cactcagcat agcaaaggta tgtcccagac tatcatgcaa atcctgaccg atacgactac
     2881 gttcattttc agcaagcaat agatttatct gagcattttg cttgacctga gcttctttca
     2941 aatcctcgac aatacgaatc cgaaccaatc caaaagtcat taaatcgaca aaagtaagaa
     3001 ttacaagtag atagaataga aactcaactt cgattctctg aaaaatcaac agttgcccca
     3061 caacaaggac ttgagcaaga agaaaagtcc agacatgtaa agactttaaa ctacgtacgc
     3121 tgaaatgata acttaagaga ttggatagga aaaagaaaaa ccagatataa ttaacagcaa
     3181 caaaggcagt attcccaact acataagtca gcatgaggcc ccaatatagc caagataggc
     3241 gctggctctt agttgttaaa acacccaaat atgccactac aaatagaata tcaatcaata
     3301 aatgccaggc agaaagccac ccagtcacta cagacaggat ggggaaaatc ataaaaatta
     3361 aactgatcca aaacatataa tgtattcttt tcagtctttc aagcattaag cattctcctt
     3421 atgaccttga aggtaaatgg tcaaaccaaa caaaactact gaaaaaacaa gtaaataaac
     3481 tgtggctgat agattgatgc caccctcatt taagaaggtc ttgagcaact ccatcaactg
     3541 ataggtcggg agacacttac ctactacttg catccagtct ggaaataaag agataggcat
     3601 ccagagtcca cctaaaacag ccaaccctag ataaagaaga ttgcccacga cagacatcaa
     3661 ctgactagtt ggtaagagag tcaaggtcaa accaagcgct acgaaggcaa tacttcctac
     3721 tatcagcaaa agtgcagccc caatccaatt tccaagagac atgtccacac ctcttacaaa
     3781 atgcccaact gagaaaacca ccaagattga aaccaaataa tcaaccagca tacttgttat
     3841 ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt tctgccagtt
     3901 gttgatcttg tcggtatgta aaacaactgg gaatgagaag atagctgttg acatcatgga
     3961 aaatgcagtc atggagataa gataatcacg cataaaattc gcgagttcac ctggtgtgtc
     4021 ctgatagata ccagaaaaaa ataaatagaa agccgtcggc atccctactg acaatagata
     4081 atagatcaat tgtcgtttgg tcaataaaaa ttctatctta ctaagtgcta gccatcgttt
     4141 catcttagtt atctcccttc tgcgtttctt caaagattgt atccaacaaa ctacgattat
     4201 taacttcaat ttcttgtatg ccacatcctg cttgaactaa cagttcccaa aaagcatctg
     4261 cttcgcgtgt gactacttgt agagcatcct gtttttgtga ccagttttca accaagttag
     4321 actgctcaat gacttccttg tatgccagag gaaggataaa atgcttttca attccctcac
     4381 tacgcatagc tagaggcgtc gtatcacgaa tcaactctcc cttatttaaa accaaaatcc
     4441 ggtcagccgt atgctctacc tcttcaatat aatgagacga atagagaatc gtgactcctt
     4501 gcgcttttag gtcccgaacg atttcccaaa agcgttgacg agttgaagta tccatggcag
     4561 cagttggttc atctaaaaag acaagctttg gtcgcccaat caaggtcaag acaaaagaga
     4621 agagacgctt ttgcccgcct gacaattttt ctgcgaattg ctctttttgt tgctggtcaa
     4681 actgcaatag ttgatcgatt tcctgatcgc tcaaggaatt tggatagata cgttgaaaga
     4741 aagcaatcaa ctctttgacc tttaatttct gaacgatgac attttcttga ggcagataac
     4801 ctctaatata gtctaactga gaactcgtca ctgacaagcc ttggatggat acttgaccgc
     4861 ttgtgaccag tttatctcca agcagacagt ccaagagtgt ggtcttccca gcaccattgg
     4921 gcccaatcaa ggcgacgcat tcaccttcag ctacctcaaa ggaaataccc ttcaaaatag
     4981 ccttgccctt gatgttttta tttaggcttt ctaccttaat catattcatg atattctcct
     5041 ttcaaccact ccattctcat aaggaaaacg acgaaaatca taaatccaaa ccccaaagca
     5101 ccacgaatga attggcgaag caaggtttgg tcaaaccaac ctgtaaacat ttccactaac
     5161 cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat tcctcaagct
     5221 cctttttcac atctccgact aatttcaaac cttctctaac aagccaagac atcattccaa
     5281 agccagcaaa gagctcccaa ggaaaatgat agaaactctc atccaatccc gaaaacatga
     5341 gttaggtcat aactcctgct actactaaac tcactgcgat aatcatttta tttctcatct
     5401 cttcttcctc catttcatac tacaattata gtcttttgaa atcagaggag acagaagctt
     5461 ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa gcaagataca
     5521 ctatacaata aaacacaatt agaaaaatct aaggcaactt cctcaaaaga gatatcaaac
     5581 ccaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt agaaattttt
     5641 atcagacaaa cacatatata gtgtattgaa tctataacag taggccttaa atactaaaat
     5701 atttctataa attaatttaa ctttcctgat agagctgttc atatcttatt tcaattctct
     5761 aaattatacg ttgaacaaaa cccttctatt tctttcttaa agatttataa gagttataaa
     5821 atctgttaaa tttcaatgtg tatacctaaa ctacggtatt tattgaaaag actggagaca
     5881 aaaagtatac gctgccaaaa tgaattactg aaaatcaaaa aagagagaac caaactgatt
     5941 ccctcttaat gtatataata tctagtttta aaaatacaca ctcacatatc tctgt
//
LOCUS       DBDESG010000084         5971 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.084, whole genome shotgun sequence.
ACCESSION   DBDESG010000084 DBDESG010000000
VERSION     DBDESG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.084"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..898
                     /gene="purB"
                     /locus_tag="ACKB38_002054"
     CDS             <1..898
                     /gene="purB"
                     /locus_tag="ACKB38_002054"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGL7980755.1"
                     /translation="IMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAAGVEAG
                     KISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERM
                     ATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYEN
                     VALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMIRNMNSTFGLI
                     FSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVTSRLTQEEIDE
                     IFNPVYYTKRVDDIFERLGLGD"
     gene            complement(953..4891)
                     /gene="strH"
                     /locus_tag="ACKB38_002055"
     CDS             complement(953..4891)
                     /gene="strH"
                     /locus_tag="ACKB38_002055"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGL7980756.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPATEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAVTHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTCENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKVLPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(5219..5935)
                     /locus_tag="ACKB38_002056"
     CDS             complement(5219..5935)
                     /locus_tag="ACKB38_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGL7980757.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         1506 a         1167 c         1282 g         2016 t
ORIGIN      
        1 catcatgatg gggcgtactc atggtgtgca cgctgagccg acaacctttg gtcttaaatt
       61 agcaacttgg tacagcgaaa tgaaacgcaa tatcgagcgc ttcgagcatg cggctgctgg
      121 tgtagaagct ggtaagattt ctggtgcggt tgggaacttt gccaatatcc caccatttgt
      181 agaggagtat gtctgcgata aacttggcat ccgtgcccaa gaaatctcta cacaagtcct
      241 tcctcgtgac cttcacgctg agtactttgc ggttcttgcc agcattgcga cttcaatcga
      301 acgtatggcg actgagattc gtggtctaca aaaatctgag caacgcgaag tagaagagtt
      361 ctttgctaaa gggcaaaaag ggtcttcagc aatgcctcac aaacgcaacc caatcggttc
      421 tgaaaatatg actggtctgg cgcgtgtcat tcgtggtcac atgattacgg cttatgaaaa
      481 cgtcgctctc tggcatgaac gcgatatttc tcactcatca gctgagcgta tcatcacacc
      541 agatacgacc attttgattg actacatgct caaccgtttt ggaaatatcg tcaagaactt
      601 gacagtcttc ccagaaaata tgatccgaaa catgaactcg acttttggtc ttatctttag
      661 ccaacgggct atgttgacat tgattgaaaa aggcatgacc cgtgagcaag cctatgactt
      721 ggtgcaacca aaaacagcct actcttggga caaccaagta gactttaaac cacttcttga
      781 ggcagattca gaagtaacat cacgtctcac acaagaagaa atcgatgaaa tcttcaaccc
      841 agtttattac accaaacgag tggatgatat ctttgaacgt cttggactag gtgattaatt
      901 aaaaaataaa cagcgagctt caatctcgct gtttattttt tatcgaaaag acttagtctt
      961 cttttctttt agtgagtcca taggctgcta gtgtggacat gagtcctgcg actactagtc
     1021 ctgcagaatc gtgagttcct gtttcaggaa gttttttctc tgttaccaca ggagctggat
     1081 cttgaggaag aactttgctt tcctcagcag gagcagttga tggagctggt tggcttggga
     1141 tttctagttt tggtttttct tcagcaatag cggcttgtcc gttttcatcg cctacatgtg
     1201 ttaccatagt tccgacttcg actatttgag taacggcttc ctgtgctacg acactattta
     1261 caagtgtttt cacttcctta ccatcggcag aagtgctcac agagtagaag ttgctacgat
     1321 gtccattgac gcccttagta atgacttgtg tttttccttt gagtaagagt ggattttcac
     1381 aagtcactgt ggtaaatgga atttcttctt cttggatatc cagtctaggt tttacctcag
     1441 tagttggtgc aagaccactt tcatcaccct tgtgagttac aggagcgcca acttcaacca
     1501 cttggtttat aacttctttg gttacctggc tatcaaggac tgtttctgtt gtttttccat
     1561 tttcagtgag tacagagatg taatgagttc gttcaccttt gactcctgct gtgataatat
     1621 tttcctgacc ggctgggagg ttaggatttt ctttcttgat aacttcaaat ggaatttctt
     1681 cagttcttgt gatgagttct ggtctggttt caacattggc agccacttca ttttcatcta
     1741 ggcttcctga atgagttaca gctggtttga ggccttgaag agcggctttt aggttggcta
     1801 caagcgtgtc aagctcagct tgtttattac ggttgaggtt gtaatttaga gctgttttag
     1861 ctgcgtcaag ggcctcaaga ctttctttac tatatccttc taagtttgta ggaattttag
     1921 ctaattcttc gcggagagca ttataattag cacgaaagta gtctttgttg tggtctgcaa
     1981 aggcagtcat gagttcaaag atttcctctt ccttgtattc agcgcttggt ctatctgccc
     2041 agattgaaag catacttccg actgttggaa gatctacttc aggatatttg gtagaagcta
     2101 gttgattgaa tggtgttttt ccagtattct caatagcttt cttgaggaaa ccaccaccat
     2161 cttctggttt ttgaccaaga atgtagtacc agtcaccgtt ggtattcaag aatttatagc
     2221 ctttgcttgc taggtattga ggtgatgcga ggttatatcc ccaccagcct ttagaccagt
     2281 aagaaatcaa gacatctttg tcaaactgaa catcgtcctt gtcttcatag tagaagccat
     2341 cgttgaaggc cattggttga agccctcttt ctttggccat agctgcgagg gtgttggcat
     2401 attcggcaaa tttgccatag agttgatacc acttgaggta gtaccagcct tgggcactag
     2461 tcgcatcgtt ggcgtattcg tcagtaccaa agttgaaaat ctttgtttta cctgcaaaga
     2521 agtccatgta tttaccgatg agggctttta caaagttcat cgcttcttcg tttttcaagt
     2581 ccatagttgt ttttgaaact ttatcaaagt gggcttgagg atttttaata cctaattttt
     2641 ccatggcaac cagcatagca tccatgtgac ctggactgtt aatagctggg atgagaccga
     2701 tgtccttaga tttagcgtat tcaattagct ctgttacttc tgcctgtgtt agtgcagtac
     2761 cgtttggatc gtcgtagtaa gctttagttc cttcgataat agctttttta acgtcatcac
     2821 tagcataggt ttttccgttg gcagtaatgg tcatatcatc gagtagaaag cgaagtccgt
     2881 catttcctag aaggagatgg acatcagaat atccgagctc actggccttg tctacgatgc
     2941 gtttgagctg gttcagagta aagtatttgc gtccagcatc gattgagatt accttgtttt
     3001 tggcaagttt ttcaacctca cgtttagctt cttcttcttt ttgagcttca ggcgtgaggg
     3061 tcaagttgtt gacagtttct tgaagtttag caatggcttg atcaatcgta tcttgttggg
     3121 cacggctaag gttgctatcg agagagcgaa tagctttttc agcttctttt acggccgtga
     3181 cgctttctgc agtataacgg ttcaggtctt ttggtacctc gttaagtgct tgctctgcag
     3241 attcataatc agctgcgaag tattcagcgt tggcatttgc aaaatgacgc atgagtttga
     3301 agaggcgtga tggtgaataa cgtgcagatg gagtgtcagc ccaagcagct accataccac
     3361 cgatgattgg gatatcagct ccttctgttt ttggtacaga agtgattggt gtgtttttaa
     3421 taccattgag cccctgatcg agattgtacc agccttggcc atcagcgttt cgtccaagaa
     3481 cgtagtacca agcatcattg gtattaagga tttggtgacc tttttcagct agtagtttag
     3541 aagaagcgac atcgtagcct ccccaaccac cagtccacat agaaacgatg atgtctttgt
     3601 caaaactacc aaagcttgtg tcgctattgt agtagatacc gtcgttaaaa gccattggtt
     3661 tgagaccgtg cgattttaca atacgagcga ggtcattggc gtaggcaata aatttttcat
     3721 agccttttac agggtagcct tcgtttggat agtatttatc agcttgaagc acactccaac
     3781 ctttagcatc tgtcgcatca ttggcatatt catcaagtcc gatgttgaag atttcagtct
     3841 ttttcgcgaa ataagcagca tacttgtcga taagggcttt tgtaaaagcg acagcttgtt
     3901 cgttgtcaag atcgacagta cgggctgatt tcttcccaaa atagctaaag ttagggtttt
     3961 ggattcccaa ttctttcatg gcattgagaa tcgcatccat gtgtccagga ctatttactg
     4021 tcggaatgag accgatacct ttatctttgg catagttaat cagatctgtc atttgacttt
     4081 ctgttaagtg attgccgttt ggatcgttgt aataatcatt tgtacctttt tcaatggcgc
     4141 gtttgacatc gtcactggca taggtcttgc cgttagctgt gatgctcata tcgtccaaca
     4201 tgaaacggag tccatcattt ccgactaata ggtgtaaatc agtgtagcca taatgtttcg
     4261 ctttatcgat gatttccttg agctgttctg gtgagaaata tttacgtcca gcatcaatag
     4321 aaacaatttt ctttttcgct agtttttcat ttacagttgc agcacgttcc tttcctgcct
     4381 ctgttgccgg tttgtcagcc tctgctttcg cttcatcttt tttagctggt ttatccttgt
     4441 cagtcttgtc tgtatttgac tctttagaat caacctcttt cgcttcttcc tttttagggc
     4501 tagcttcttc tgccttttta ttagcagttt ctttttcagc agaagttgga gttaccactt
     4561 ctgctttatc actaggagtt gaactaactt cctcttgtgg tttttcttct gtttttggaa
     4621 gactagctac cttatcagta gctggagttt ctgtttctac agtttttgga gcttctggtt
     4681 gaagcactgc tttaggtgtt tcctcagtcc gattttcgga tgattgaggg gaatcagaaa
     4741 ccgtatggat ggtcggttgg ttttctgtag tagtaggagt aactccatcg gctgcaacag
     4801 tctgtgcttg gaaggcaaat ccaattagaa cagaagctgc tcctacagcg tatttacgaa
     4861 tagaaaaacg ctgttgtttt tcatgtttca ttgcaaaacc tcctgattgc attgttatat
     4921 tgatagcgat tatataaatc aacgccttta ttttatttct tatattaatt tcttatatta
     4981 acgagagtca agaggagatg acaaaaaact ataataagta taaaaaaata taaaatttaa
     5041 acttaagatt tcagattggt cggaaaaaat acgtatatat atctagtata atttttggtt
     5101 ctatttctat aaaatattcc acaaattata gaattttcca aaaataggta agcgctacct
     5161 ttttggtgta gtataataag catagaaaaa gcccaagcga ttagctcagg ttttcttctt
     5221 agtgatcacg gtcacatgag ataaatttaa tcttgtagta atcagatcgt ttgtaagttt
     5281 cactgtattc taaaacttgg ccagttgatt cgagtttggt gattttagtt tgtaggacag
     5341 tagggaattg ttcatcgact ccgaggactg aagctgcatg ttctggagtt ggaaagacta
     5401 tttcgttgat ttcttcaaag tgttcatcat tcatgtgaat gtggtagtct aacttgaaac
     5461 gattatagat agaactatag tattcaaggt ttggataatt tgcgttgata tattgttctg
     5521 ggatgtagga tgtatggtag atataaacga caccgtttga ttcgcggata cgttcaatct
     5581 tgtagtagaa ttgatcgccg cgtagaccca atttttccaa gtaaacaagc ttgtttccgc
     5641 gttcaattga aagaacagtt accttatcat ctttagcatt gaagagttca atatctgaaa
     5701 actctacaag cttgtgtttg cgtgcacgtg aaacgaaggt tccttttcct tgttggcgga
     5761 caatatagcc atctttggca aggtcgttta aggcgcgaac aactgtgata gagctgacat
     5821 cgtacattga aatgagttct gcttcagtgt aaaatttatc tccactgcta aactgcccag
     5881 agatgatttt attttttaat tcgtctttta tgtattgata tttgggaatt gccatatttt
     5941 tacctctatt ttccttcttc catagaaaat t
//
LOCUS       DBDESG010000085         5704 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.085, whole genome shotgun sequence.
ACCESSION   DBDESG010000085 DBDESG010000000
VERSION     DBDESG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.085"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            170..370
                     /locus_tag="ACKB38_002057"
     CDS             170..370
                     /locus_tag="ACKB38_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980758.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            412..816
                     /locus_tag="ACKB38_002058"
     CDS             412..816
                     /locus_tag="ACKB38_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HGL7980759.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1047..1754
                     /gene="ccdA2"
                     /locus_tag="ACKB38_002059"
     CDS             1047..1754
                     /gene="ccdA2"
                     /locus_tag="ACKB38_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGL7980760.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1765..2322
                     /gene="sdbB"
                     /locus_tag="ACKB38_002060"
     CDS             1765..2322
                     /gene="sdbB"
                     /locus_tag="ACKB38_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGL7980761.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2696..4087
                     /locus_tag="ACKB38_002061"
     CDS             2696..4087
                     /locus_tag="ACKB38_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGL7980762.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4230..5147
                     /gene="adcAII"
                     /locus_tag="ACKB38_002062"
     CDS             4230..5147
                     /gene="adcAII"
                     /locus_tag="ACKB38_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGL7980763.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5155..>5704
                     /locus_tag="ACKB38_002063"
     CDS             5155..>5704
                     /locus_tag="ACKB38_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGL7980764.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYT"
BASE COUNT         1741 a         1014 c         1224 g         1725 t
ORIGIN      
        1 gtaaaatgaa ataagaatag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagt ccatatgtac tattctagtt tcaatatacc ataactgttc ctagtcaacc
      121 tgcaagccct gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag
      181 cgcttcggct agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccgaa
      301 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgggctgc cttggactct
      421 ctcaaagatg tgaaagcaaa tgctagcgac agcaagcctg cacaggacaa gaaggatgca
      481 aaacaaggaa cggaagatag taaggattca gataagatga ctgaaacaaa ctcagttccg
      541 gcaggagtga ttgtggtcag tctacttgcc ctcctaggcg tgattgcctt ctggctgatt
      601 cgccgtaaga aagagtcaga aatccagcaa ttaagcacgg aattgatcaa ggttctagga
      661 cagctagatg cagaaaaagc ggataaaaaa gtccttgcca aagcccaaaa ccttctccaa
      721 gaaacccttg atttcgtgaa agaagaaaat ggctcagcag agacagaaac taaactagta
      781 gaggagctta aagcaatcct tgacaaactc aagtaaaaca tgatttatga aatgtcagac
      841 ctaaaagaca ttttcgttca caaaagcagc gatgagtaat ccttgtcgtt gctttttggc
      901 tattttgggg gataagatcc gccttgacta gcaaaagaaa agattggtcc aatagtgccc
      961 gatacgattt tgcgtagcag aaagcttaca aataaaattg aatatagtaa aataggatta
     1021 gaattattaa gaaagttggt tctttattgg aaacgatagt atttttaatc tctgtttttc
     1081 tagcaggtgt tttatccttt ttttctcctt gtatttttcc tcttctgcca gtctatgctg
     1141 ggattttatt ggatgatcaa gaaagtgcaa aaagcttttc tttgtttggg agaaaggttc
     1201 tctggtcagg cttgattcga acactttgct ttatcgctgg tatctctctc attttcttta
     1261 ttctaggctt tggtgctggt tactttggtc atattctcta tgcaaattgg tttcgatatg
     1321 gcatgggagc tattattatc attttgggtc ttcaccagat ggaaattttt catttgaaga
     1381 aattagaagt tcaaaaaagt tttaccttta aaaaatcaga ttctaatcgt tattggtcag
     1441 cttttttact tggtattacc tttagctttg gttggacacc ttgtattggt ccagttttaa
     1501 gttctgtttt agcacttgcg gcttctggag gcaatggcgc ttggcaagga gcgatttata
     1561 ctctcattta cactctgggc atggcccttc ctttcttggt attggcacta gcttcaggtc
     1621 tagtcatgcc atattttagt aaaatcaagc gtcatatgat gctactaaag aaaattggtg
     1681 gtttcctcat tgttttaatg ggaattttgt tactattagg acaagtaaat gttctagctg
     1741 gaatttttga ataaaggagg acaaatgaaa aaagtaatgt ttgctggctt aagtctcttg
     1801 tcattagttg tattgatggc ctgtggtgag gaagaaacta aaaagactca agcagcacaa
     1861 cagccaaaac aacaaacgac tgtacaacaa attgctgttg gaaaagatgc tccagacttc
     1921 acattgcaat ccatggatgg caaagaagtt aagttatctg attttaaggg taaaaaggtt
     1981 tacttgaagt tttgggcttc atggtgtggt ccatgcaaga aaagtatgcc agagttgatg
     2041 gaactagcgg cgaaaccaga tcgtgatttc gaaattctta ctgtcattgc accaggaatt
     2101 caaggtgaaa aaactgttga gcaattccca caatggttcc aggaacaagg atataaggat
     2161 atcccagttc tttatgatac caaagcaacc accttccaag cttatcaaat tcgaagcatt
     2221 cctacagaat atttaattga tagccaagga aagattggaa agattcaatt tggtgctatc
     2281 agtaatgcgg atgcagaagc agcatttaaa gaaatgaact agaatcgata aaatctgatt
     2341 acaagatgta gtcagatttt ggtaacttct aaaactaact tccagtttcc cacaacctag
     2401 cttttagtat gagaaaaacg cgtgacatca gtggaaatcc gtcttagact aacttccact
     2461 ggttttcttt ttcttgatat ataagggttc aaaagcgaaa agactcagaa aaagtgcacg
     2521 acaaatagcc ctaaaacaga gtcgtcttag agtaacttcc agttttggga gagagatgga
     2581 agttactttg agaagttacg gtagtcagat ttttttagaa aaacatttta taaatattca
     2641 catatctcct atatttatgg taaaatagaa ttatcagttt attttggagt caaagatgaa
     2701 tatatttaga acaaagaatg ttagtttaga taaaacagag atgcataggc atttgaagtt
     2761 atgggatttg attttgctgg gtatcggagc catggtaggg acaggcgtct ttacaatcac
     2821 aggtactgca gctgcaacac ttgctggccc agccctagtg atttcaatcg ttatttctgc
     2881 cttgtgtgtg ggattatcag ccctcttttt tgcagaattt gcctcgcgag tacccgctac
     2941 aggaggtgcc tatagttacc tctatgctat cttaggagaa ttccctgcct ggttggctgg
     3001 ttggttaacc atgatggagt tcatgacagc catatcaggc gtagcttcgg gttgggcagc
     3061 ttattttaaa ggcttgctct ctcaatacgg gatagccttt cctcaggctt taaatggtac
     3121 ctttaatcct caagcaggga catttgttga tttattgcct attcttgtct tggtcttggt
     3181 gacttcgctt gttttactaa atgccaaggc agccttacgc tttaattcga ttctagttat
     3241 tttgaaattt tccgctttag ctctctttgt tttagtagga atttggaata ttaagtttga
     3301 taattggagc aattttgctc cttacggttt tggacaaatc tatggagcta gcactggtat
     3361 tatggctggt gcgtccttga tgttcttcgg ttttttggga tttgaatcca tctctatggc
     3421 tgtagatgaa gtcaagactc ctcaaaaaaa tattcctaga gggattgtct tatcgctttc
     3481 tatcgtaacc attctctatg ccttggtgac ccttgttttg actggtgtgg ttcactatag
     3541 tcatttaaat gtcgatgatg ccgttgcctt tgcccttcgt agtgttggga ttagttgggc
     3601 agccaactat gtgtcattag tggctatctt gaccttgatt acagtttgta tttcgatgac
     3661 ctatgcccta tcgcgtatga tttacagttt agcacgtgac ggcttagtgc ctgctgcctt
     3721 taaggaactg acgaagacta gcaagatacc aaagaatgct actatcttga caggtctagc
     3781 ttcagcagta gcagcaggaa tgttcccact agccagtatt gcagcctttt taaatatttg
     3841 taccttagcc tacttgatca tgttggctta tggtctgatt cgcttacgga aagaaaaagg
     3901 aatgcccaaa gctggggaat ttaaaacacc attggtacct ctgttaccaa ttttatcgat
     3961 catcatttgt ttatccttca tgttacagta taatatgaat acctggattg ctttcctagt
     4021 tgcgttgtta gtaggaagta ttatttactt cacttatggc tataagcatt ctaccataga
     4081 agaataaagt gaaagtctat tgagttgttg acactcagta gatttttata tgtgaaagaa
     4141 atttcaaatt ttttctaaaa atatcttgac agattaaatt ttcaggagta gaatatttac
     4201 tagttaatta aatggttaag gagttgttca tgaagaaaca aaatttattt ttagtcctgt
     4261 taagtgtctt tcttttgtgc ttgggggctt gtggtcaaaa ggaaagtcag acaggaaagg
     4321 ggatgaaaat tgtgaccagt ttttatccta tctacgctat ggttaaggaa gtatctggtg
     4381 acttgaatga tgttcggatg attcagtcaa gtagtggtat tcactccttt gaaccttcgg
     4441 caaatgatat cgcagccatc tatgatgcag atgtctttgt ttaccattct catacactcg
     4501 aatcttgggc aggaagtctg gatccaaatc taaaaaaatc caaagtgaag gtcttagagg
     4561 cttctgaggg aatgaccttg gaacgtgtcc ctggactaga ggatgtggaa gcaggggatg
     4621 gagttgatga aaaaacgctc tatgaccctc acacatggct agatcctgaa aaagctggag
     4681 aagaagccca aattatcgct gataaacttt cagaggtgga tagtgagcat aaagagactt
     4741 atcaaaaaaa tgcgcaagcc tttatcaaaa aagctcagga attgactaag aaattccaac
     4801 caaaatttga aaaagcgact cagaaaacat ttgtaacaca acatacagcc ttttcttatc
     4861 tagcgaagag atttgggctt aatcaacttg gtattgcagg tatctctcct gaacaagaac
     4921 caagtccacg acaactaaca gaaattcagg aatttgttaa gacctataag gttaaaacga
     4981 tttttacaga aagtaacgct tcttcaaaag tagctgaaac tcttgtcaaa tcaacaggtg
     5041 tgggtcttaa aactctgaat cctttagagt cagacccaca aaatgacaag acctatttag
     5101 aaaatcttga agaaaatatg agtattctag cagaagaatt aaagtgagga aagaatgaaa
     5161 attaataaaa aatatctagc aggttcagtg gcagtccttg ccctaagtgt ttgttcctat
     5221 gaacttggtc gtcaccaagc tggtcaggtt aagaaagagt ctaatcgagt ttcttatata
     5281 gatggtgatc aggctggtca aaaggcagaa aacttgacac cagatgaagt cagtaagagg
     5341 gaggggatca acgccgaaca aatcgtcatc aagattacgg atcaaggtta tgtgacctct
     5401 catggagacc attatcatta ctataatggc aaggtccctt atgatgccat catcagtgaa
     5461 gagctcctca tgaaagatcc gaattatcag ttgaaggatt cagacattgt caatgaaatc
     5521 aagggtggtt atgttatcaa ggtagatgga aaatactatg tttaccttaa ggatgcagct
     5581 catgcggata atattcggac aaaagaagag attaaacgtc agaagcagga acacagtcat
     5641 aatcacgggg gtggttctaa cgatcaagca gtagttgcag ccagagccca aggacgctat
     5701 acaa
//
LOCUS       DBDESG010000086         4879 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.086, whole genome shotgun sequence.
ACCESSION   DBDESG010000086 DBDESG010000000
VERSION     DBDESG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.086"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            64..426
                     /locus_tag="ACKB38_002064"
     CDS             64..426
                     /locus_tag="ACKB38_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980765.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKIFVTVIGYFEINIDENITDILYVNGTAILYLYLRSIVSIVSAIDSSEAMLL
                     PIINVLELLDKSQPFEEE"
     gene            825..1070
                     /locus_tag="ACKB38_002065"
     CDS             825..1070
                     /locus_tag="ACKB38_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980766.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFIKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            1471..2463
                     /locus_tag="ACKB38_002066"
     CDS             1471..2463
                     /locus_tag="ACKB38_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7980767.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2465..3283
                     /locus_tag="ACKB38_002067"
     CDS             2465..3283
                     /locus_tag="ACKB38_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980768.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3285..4070
                     /locus_tag="ACKB38_002068"
     CDS             3285..4070
                     /locus_tag="ACKB38_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGL7980769.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4109..4387
                     /locus_tag="ACKB38_002069"
     CDS             4109..4387
                     /locus_tag="ACKB38_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGL7980770.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4372..4569
                     /locus_tag="ACKB38_002070"
     CDS             4372..4569
                     /locus_tag="ACKB38_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980771.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1403 a          796 c         1025 g         1655 t
ORIGIN      
        1 tgttttgagc aacctgcggc tagtttccta gtttgctctt tggttttcat tgagtataac
       61 acattgttag aagttggttt aaatttccta atcagtttgt tcacatttac attcgatata
      121 ttatatccca tagttaaggt tggtcataca gatgattata gtcatggagc cgtaaaactt
      181 agtgtttctt tagttgacaa agatgccatg aaaaaaatat ttgtaactgt aataggatat
      241 tttgaaataa atatagatga aaatatcacc gatattctat acgtaaatgg tactgctatt
      301 ctttatcttt atttacgttc aattgtttca atagtttcgg caattgatag cagtgaagca
      361 atgttgctac ctatcattaa tgttttagag ttactagata aatctcaacc ttttgaagaa
      421 gaataattta ttagctcact aaattgaggg taaggaaaag taaaagcagt aagaaaaatg
      481 tcttgcatta tacagcaacc ttttgggaat gagtggatgg attgaataaa atttgattaa
      541 gagtggatga tttatctgta gattattatt ggacagttag tcttgaagta gtctaagaat
      601 taggttataa tcagtagaag ccttgctaat aatgaggagg ttagtttatg tatagtagac
      661 tgaatctaaa atagtacgaa acaattgcta aaacatttat agaaattaat tttactttcc
      721 caatcgattt gttctcatct tatttcactc cgctatatat tatggtatcg aatcttcatc
      781 agaatgataa aattaatcaa ttgatatctg attacaaaca gaatatgaaa gctttttata
      841 tcactattga aaaatttata cgagatgatg aaagccttaa gtgttatttt ataaaggtta
      901 tttcaagtcg ttccaaggta acaagtctag atcagattga agctgataaa acgatacaaa
      961 gaaaatattc aagtgagcta aaaaaattta ttggatttta taatgagatt atttgtgagg
     1021 aaaatagttt cctacatgta cgaaagaggt ggtcgagttg gtttaggtag tcgatgcgtg
     1081 agttgataat tctcagggta tggacttctt tttcatgaat gaggtaaaag agcaggtatt
     1141 gtttagagac aatcattctg agcatatttt ctggatagag ggagtatccg attttatgat
     1201 caaagttaat accgccctct ggtgagaaga tgagtaggtt ggtaatttaa actattaaac
     1261 agaatttttg attaaaagta ttatttcatg agagaaatcc taatttcaca atccataggc
     1321 aaacgcttgc atttcgtttt ttattggact ataataggtt ggtataaagc cttctgtagt
     1381 aataaaatgt agaaggtgta gaaagtaagg atttagaata tttgtagtta aaaacacaat
     1441 gttgctattc cttacgatag ggagatagat atggcaatga tagaagtgga acatcttcag
     1501 aaaaattttg tgaagactgt taaggaaccg ggcttgaagg gggctttgcg ctcctttatt
     1561 catcctgaaa agcagacctt tgaagcggtc aaggatttga cctttgaggt tccaaaaggg
     1621 cagattttag gatttatcgg ggcaaatggt gctgggaagt cgacaaccat taaaatgctg
     1681 acaggaattt tgaaaccaac atctggtttt tgtcggatta acggcaagat tccccaggac
     1741 aatcggcaag attatgtcaa agatattggc gtagtctttg gacaacgcac ccagctatgg
     1801 tgggatttgg ctctgcaaga gacctacact gtcttaaaag agatttatga tgtgccagac
     1861 tcgctctttc ataagcgtat ggactttttg aatgaagtct tggatttgaa ggactttatc
     1921 aaggatcccg tgcggactct ttcactggga caacggatgc gggcggatat tgcggcctcc
     1981 ttgctccaca atcccaaggt tcttttttta gatgagccga ccattggttt ggacgtttcg
     2041 gttaaggata atattcgtcg ggcaattact cagatcaatc aagaggaaga aactaccatt
     2101 cttttgacca ctcacgattt gagtgatatt gagcaacttt gtgatcggat tttcatgatt
     2161 gacaaggggc aagagatttt tgatggaacg gtgagccaac tcaaggagac ctttggtaag
     2221 atgaagactc tctcttttga actgctacca ggtcaaagtc atctcgtctc tcactatgac
     2281 ggtctgtctg atatgaccat tgatagacaa ggaaacagcc tcaacattga atttgatagt
     2341 tctcgctacc agtcagctga cattatcaag caaaccctgt ctgattttga aatccgcgat
     2401 ttgaagatgg tggatacgga tattgaggat attatccgtc gcttctaccg aaaggagctc
     2461 taggatgatc aaattgtgga gacgttataa accctttatc aatgcagggg ttcaggagtt
     2521 gattacttac cgagtcaact ttattctcta tcggattggc gatgtcatgg gggcttttgt
     2581 ggccttttat ctctggaagg ctgtctttga ttcttcgcaa gagtctttga ttcagggctt
     2641 cagtatggcg gatatcaccc tctacatcat catgagtttt gtgaccaatc ttctgactag
     2701 atccgattcg tcctttatga ttggggagga ggtcaaggat ggctccatta tcatgcgttt
     2761 gttgcgacca gtgcattttg cggcctccta tcttttcacc gagcttggtt ccaagtggtt
     2821 gatttttatc agcgttggcc ttccattttt aagtgtcatt gtcttgatga aaatcatatc
     2881 gggtcaaggt attgtagagg tgctaggatt aactgtcatt tatcttttta gcttaacgct
     2941 cgcctatctg attaactttt tctttaatat ttgctttgga ttttcagcct ttgtgtttaa
     3001 aaatctttgg ggttccaacc tacttaagac ttccatagtg gcttttatgt cggggagttt
     3061 gattcccttg gcattttttc caaaggttgt ttcagatatt ctctcctttt tgcctttttc
     3121 atccttgatt tatactccag ttatgatcat tgttggaaaa tacgatgcca gtcagattct
     3181 tcaggcactc cttttgcagt tcttctggct cttagtgatg gtgggattgt ctcagttaat
     3241 ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt tagtatgaaa aaatatcaac
     3301 gaatgcatct gatttttatc agacaataca tcaaacaaat catggaatat aaggtagatt
     3361 ttgtggttgg tgtcttggga gtctttctga ctcaaggctt gaatctcttg tttctcaatg
     3421 tcatctttca acatattcca ttcctagaag gctggacctt tcaagagata gctttcattt
     3481 atggattttc cttgattccc aagggaatgg accatctctt ttttgacaat ctctgggcac
     3541 tagggcaacg cctagtccga aaaggggagt ttgacaagta tctgactcgt cccatcaatc
     3601 ctctctttca catcctagtt gaaacctttc agattgatgc cttgggtgaa ctcttagtcg
     3661 gtggtatttt attgggaaca acagtgacca gcattgtttg gactcttcca aaattcctgc
     3721 ttttcctagt ttgtattcct tttgcgacct tgatttatac ttctcttaaa atcgcaacag
     3781 ccagtatcgc cttttggact aagcagtcag gcgccatgat ttacatcttc tatatgttca
     3841 atgactttgc taagtatccg atttctattt acaattctct tcttcgttgg ttgattagct
     3901 ttatcgtgcc tttcgccttt acagcctact atccagctag ctatttctta caggaaaagg
     3961 atgtgttctt taacgtagga ggtttgatgt tgatttctct ggttttcttt gttatttccc
     4021 ttaaactttg ggataagggc ttagattcct acgaaagtgc gggttcgtaa aagctaaagt
     4081 aagactaaaa tcaagaaaga aacttatgat gtttgtaatt gaagaagtca aggatgaaaa
     4141 tcaaaaaaag gcagttgtcg ctgaggtttt gaaggatttg ccagaatggt ttggaatccc
     4201 agaaagcaca caagcctata tagaaggaac cacgacactg caagtttgga ccgcctatca
     4261 ggagagtgat ttgactagat ttgtaagctt atcctattcg agtgaagatt gtgcagagat
     4321 tgattgtctc ggcgtaaaaa agcttatcaa ggtagaaaaa ttgggagcca attgcttgct
     4381 actttagaga gtgaagctcg taaaaaagtt ggttatctgc aggtcaaaac agtggcagaa
     4441 ggttctaata aagattatga tcgaacaaat gacttttatc gaggtcttgg ctttaaaaag
     4501 ttagagattt ttcctcaact atggaatccg caaaatcctt gtcagatttt gattaaaaag
     4561 cttgaataat attacttgac atctattctc agagtgctat actgtaagtg taatcgccga
     4621 tttagcttag ttggtagagc aaggcactcg taaagcctag gttataggta gataaacgac
     4681 tgaggatttg aaaaaataga taggtagaag ataaccgtta agccttactc ttagcggtta
     4741 tttatattgt ttaatagcgc taatatttta tcaattatgc ctgttttcgt gtttctggta
     4801 gttgttcaag tttattgcta ctatttttga tggtatgaat gtgcttataa tgtatcccgg
     4861 ttaacgaaag ttttggact
//
LOCUS       DBDESG010000087         4848 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.087, whole genome shotgun sequence.
ACCESSION   DBDESG010000087 DBDESG010000000
VERSION     DBDESG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.087"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..1526
                     /locus_tag="ACKB38_002071"
     rRNA            <1..1526
                     /locus_tag="ACKB38_002071"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1578..1650
                     /locus_tag="ACKB38_002072"
     tRNA            1578..1650
                     /locus_tag="ACKB38_002072"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1611..1613,aa:Ala,seq:tgc)
     gene            1775..4676
                     /locus_tag="ACKB38_002073"
     rRNA            1775..4676
                     /locus_tag="ACKB38_002073"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4753..>4848
                     /gene="rrf"
                     /locus_tag="ACKB38_002074"
     rRNA            4753..>4848
                     /gene="rrf"
                     /locus_tag="ACKB38_002074"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1344 a          998 c         1439 g         1067 t
ORIGIN      
        1 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gaaggaggag
       61 cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctggtagc
      121 gggggataac tattggaaac gatagctaat accgcataag agtagatgtt gcatgacatt
      181 tgcttaaaag gtgcacttgc atcactacca gatggacctg cgttgtatta gctagttggt
      241 ggggtaacgg ctcaccaagg cgacgataca tagccgacct gagagggtga tcggccacac
      301 tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg
      361 gacggaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc
      421 tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac actgtgacgg tatcttacca
      481 gaaagggacg gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc
      541 cggatttatt gggcgtaaag cgagcgcagg cggttagata agtctgaagt taaaggctgt
      601 ggcttaacca tagtaggctt tggaaactgt ttaacttgag tgcaagaggg gagagtggaa
      661 ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct
      721 ctctggcttg taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc
      781 ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag accctttccg gggtttagtg
      841 ccgtagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa
      901 ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa
      961 gaaccttacc aggtcttgac atccctctga ccgctctaga gatagagctt tccttcggga
     1021 cagaggtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt
     1081 cccgcaacga gcgcaacccc tattgttagt tgccatcatt tagttgggca ctctagcgag
     1141 actgccggta ataaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga
     1201 cctgggctac acacgtgcta caatggctgg tacaacgagt cgcaagccgg tgacggcaag
     1261 ctaatctctt aaagccagtc tcagttcgga ttgtaggctg caactcgcct acatgaagtc
     1321 ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg gccttgtaca
     1381 caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta accgtaagga
     1441 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg
     1501 gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa ctgcgcattg gtcttgttta
     1561 gtcttgagag gtcttgtggg gccttagctc agctgggaga gcgcctgctt tgcacgcagg
     1621 aggtcagcgg ttcgatcccg ctaggctcca ttggtgagag atcaccaagt aatgcacatt
     1681 gaaaattgaa tatctatatc aaatagtaac aagaaaataa accgaaaacg ctgtagtatt
     1741 aataagagtt tatgactgaa aggtcagaaa ataaggttaa gttaataagg gcgcacggtg
     1801 gatgccttgg cactaggagc cgacgaagga cgtgacaaac gacgatatgc cttgggtagc
     1861 tgtaagtaag cgatgatcca gggatttccg aatgggggaa cccaacaggt aatacctgtt
     1921 acccacatct gttaaggatg tgaggaggaa gacgcagtga actgaaacat ctaagtagct
     1981 gcaggaagag aaagcaaaag cgattgcctt agtagcggcg agcgaaacgg cagaagggca
     2041 aaccgaagag tttactcttc ggggttgtag gactgcaatg tggactcaaa gattatagaa
     2101 gaatgatttg ggaagatcag ccaaagagag taatagcctc gtatttaaaa tagtctttgt
     2161 acttagcagt atcctgagta cggcgggaca cgtgaaatcc cgtcggaatc tgggaggacc
     2221 atctcccaac cctaaatact ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     2281 tgaaaagcac cccgggaggg gagtgaaata gaacccgaaa ccgtgtgcct acaacaagtt
     2341 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgttatg
     2401 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtctgaat agggcgcctt
     2461 agtatcatga cgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgcggt
     2521 aagacgcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     2581 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     2641 agcgtcgaca ttagagattc ttggaggtag agcactgttt gggtgagggg tccatcccgg
     2701 attaccaatc tcagataaac tccgaatgcc aatgaattat ggtcggcagt cagactgcga
     2761 gtgctaagat ccgtagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
     2821 attgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttag cttagaagca
     2881 gctattcatt caaagagtgc gtaatagctc actagtcgag tgaccctgcg ccgaaaatgt
     2941 accggggcta aaacaattta ccgaagctgt ggataccttt ataggtatgg taggagagcg
     3001 ttctatgtgt gatgaaggta taccgtgagg agtgctggaa cgcatagaag tgagaatgcc
     3061 ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg taagactaag gtttccaggg
     3121 gaaggctcgt ccgccctggg ttagtcggga cctaaggaga gaccgaaagg tgtatccgat
     3181 ggacaacagg ttgatattcc tgtactagag tatgtagtga tggagggacg cagtaggcta
     3241 actaaagcag acgattggaa gagtctgtct aagcagtgag gtgtgaattg agtcaaatgc
     3301 ttaattctat aacattgagc tgtgatgggg agcgaagttt agtagcgaag ttagtgacgt
     3361 cacactgcca agaaaagctt ctagcgttta aacatactct acccgtaccg caaaccgaca
     3421 caggtagtcg aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca
     3481 aaatgacccc gtaacttcgg gagaaggggt gctgacttaa agtcagccgc agtgaatagg
     3541 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     3601 ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga
     3661 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     3721 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     3781 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     3841 ccatggagct ttactgcagt ttgatattga gtgtctgtac cacatgtaca ggataggtag
     3901 gagtctaaga gatcgggacg ccagtttcga aggagacgct gttgggatac tacccttgtg
     3961 ttatggccac tctaacccag ataggtgatc cctatcggag acagtgtctg acgggcagtt
     4021 tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagaatgg
     4081 ttggaaatca ttcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     4141 gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc
     4201 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     4261 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     4321 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     4381 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     4441 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     4501 atgtagggaa gggataaacg ctgaaagcat ctaagtgtga aacccacctc aagatgagat
     4561 ttcccatgat tatatatcag taagagccct gagagatgat caggtagata ggttagaagt
     4621 ggaagtgtgg cgacacatgt agcggactaa tactaatagc tcgaggactt atccaaagta
     4681 actgagaata tgaaagcgaa cggttttctt aaattgaata gatattcaat tttgagtagg
     4741 tattactcag agttaagtga cgatagccta ggagatacac ctgtacccat gccgaacaca
     4801 gaagttaagc cctagaacgc cggaagtagt tgggggttgc cccctgtg
//
LOCUS       DBDESG010000088         4751 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.088, whole genome shotgun sequence.
ACCESSION   DBDESG010000088 DBDESG010000000
VERSION     DBDESG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.088"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            37..990
                     /gene="coiA"
                     /locus_tag="ACKB38_002075"
     CDS             37..990
                     /gene="coiA"
                     /locus_tag="ACKB38_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGL7980772.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            1009..2811
                     /gene="pepF"
                     /locus_tag="ACKB38_002076"
     CDS             1009..2811
                     /gene="pepF"
                     /locus_tag="ACKB38_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGL7980773.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYAYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            2813..3526
                     /locus_tag="ACKB38_002077"
     CDS             2813..3526
                     /locus_tag="ACKB38_002077"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGL7980774.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            3593..4534
                     /gene="prsA"
                     /locus_tag="ACKB38_002078"
     CDS             3593..4534
                     /gene="prsA"
                     /locus_tag="ACKB38_002078"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGL7980775.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(4652..>4751)
                     /locus_tag="ACKB38_002079"
     CDS             complement(4652..>4751)
                     /locus_tag="ACKB38_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGL7980776.1"
                     /translation="AIIDYIDYYNNKRIKIKLKGLSSVQYRTKSFG"
BASE COUNT         1562 a          898 c          977 g         1314 t
ORIGIN      
        1 tcttttttac gaatgatata gaaaaggagg gaattcatgt ttgttgcgag agatgctagg
       61 ggagaattgg taaatgtgtt agaggataaa cttgagaagc aagcatacac ctgcccagct
      121 tgtggaggcc agctccattt gcgtcaagga ccaagtgtac ggacgcattt tgcccataaa
      181 tccttaaaag actgtgattt tttctttgaa aatgaaagtc cagaacacct ggccaataag
      241 gaatccctct atcactggtt gaaaaaagag acaaaggttc aattagagta cccgctttca
      301 gaacttaaac agattgcgga tgtatttgta aatggcaatc tagctctaga agttcagtgt
      361 agtcccttgc ctcagaaagt ccttaaagag cgaagtgagg gctatcgtag tcagggttac
      421 caagtactgt ggttgctggg tcaaaaactg tggctcaagg agcgtttgac tcgtctacag
      481 caaggttttc tttatttcag tcaaaacatg ggcttttatg tttgggaatt agacaaggaa
      541 aaacaagttt taagactcaa atacctgatt taccaggatc tccgcggtaa actccattat
      601 caaatcaagg aattttccta tggtcaaggt agtttattgg aaatattgcg tcttccctat
      661 aagagacaaa aaatatctca ttttacagtt tctgaggaca aggacatctg tcgctatatc
      721 cggcaacaac tttattatca aaatctcttt tggatgaaag aacaagcaga agcctatcaa
      781 aagggagaaa atatcctgac ttatggactg aaagaatggt atccacaaat tcgaccaata
      841 gtgggcaaat ttttccagat tgaacaagac ttgactagct attatcagca cttttatacc
      901 tattaccaaa aaaatcctca aaatgattgg caaaagcttt atccaccagc cttttatcag
      961 caatatttct tgaaaaatat ggtagaatag aaaggatgga ggaatctaat ggtattacaa
     1021 agaaatgaaa taaatgaaaa agatacatgg gatctatcaa cgatctaccc aactgaccag
     1081 gcttgggaag aagccttaaa agatttaaca gaacaattgg agacagtagc ccagtatgaa
     1141 ggccatctct tggatagtgc ggataaccta ctagaaatca ctgaattttc tcttgaaatg
     1201 gaacgccaga tagagaagct ttacgcttat gctcatatga agaatgacca ggatacacgt
     1261 gaagctaagt atcaagagta ctatgccaag gccatgacac tctacagcca gttagaccaa
     1321 gccttttcat tctatgagcc tgaatttatg gagattagcg aaaagcagta tgctgacttt
     1381 ttagaagctc aaccaaagct gcaggtttat caacactatt ttgacaagct tttgcaaggc
     1441 aaggatcacg ttctttcaca acgtgaagaa gaattattgg ctggagctgg agaaatcttt
     1501 ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg atattgtgtt cccttatgtc
     1561 ctagacgatg atggtaaaga agttcagcta tctcatggga cttacacacg tttgatggag
     1621 tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc tttatgcgac ttacgaacaa
     1681 ttccaacaca cctatgccaa aaccttgcaa accaatgtta aggtgcaaaa ttaccgtgct
     1741 aaagttcgta actacaagag tgctcgtcat gcagccctcg cagcgaattt tgttccagaa
     1801 agtgtttatg acaatttggt agcagcagtt cgcaagcatt tgccactctt acatcgctat
     1861 cttgagcttc gttcaaaaat cttggggatt tcagatctca agatgtacga tgtctacaca
     1921 ccgctttcat ctgttgaata cagttttacc taccaagaag ccttgaaaaa agcagaagat
     1981 gctttggcag tcttgggtga ggattacttg agccgtgtta aacgtgcctt cagcgagcgt
     2041 tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg cctactctgg tggttcttat
     2101 gataccaatg cctttatgct tctcaactgg caagacaatc tggacaatct ctttactctt
     2161 gttcatgaaa caggtcacag tatgcattca agctatactc gtgaaactca gccttatgtt
     2221 tacggggatt actctatctt tttggctgag attgcctcaa ctaccaatga aaatatcttg
     2281 acggagaaat tattggaaga agtggaagac gacgcaacac gctttgctat tctcaataac
     2341 ttcctagatg gtttccgtgg aacagttttc cgccaaactc aatttgctga gtttgaacac
     2401 gccattcacc aagcagatca aaatggggag gtcttgacaa gcgatttcct aaataaactc
     2461 tacgcagact tgaaccaaga gtattatggt ttgagtaagg aagacaatcc tgaaatccaa
     2521 tacgagtggg ctcgcattcc acacttctac tataactact atgtatatca atattcaact
     2581 ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc atggtagtca agaagaccgt
     2641 gaccgctata tcgactacct caaggcaggt aagtcggact atccacttaa tgtcatgaga
     2701 aaagctggtg ttgatatgga gaaggaagac tacctcaacg atgcctttgc agtctttgaa
     2761 cgccgtttaa atgagtttga agcccttgtt gaaaaattag gattggcata aaatggttga
     2821 atcgtatagt aagaatgcta accataacat gcgtcgtcct gtcgtcaaag aagaaattgt
     2881 agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc ttgaaagaat tggaagactt
     2941 tgcccgcaag gaaaatattc ctattattcc ccatgaaacg gttgcttatt tccgttttct
     3001 tatggaaacc atgcagccta aaaatattct ggaaattggg acggctatcg gtttttcagc
     3061 tctcttgatg gctgaacatg cgccaaatgc taagattaca actattgatc gtaatccaga
     3121 aatgattggt tttgccaagg aaaattttgc ccagtttgac agtcgcaagc aaatcactct
     3181 cctagaggga gatgcggtgg atgtcttatc tacactgaca gagtcttatg atttcgtctt
     3241 tatggattct gccaagtcta aatacatcgt ctttctgcca gaaatcctca aacatttgga
     3301 agttggtggt gtggttgtct tggatgatat ttttcaaggt ggtgatgttg ccaaggatat
     3361 tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc cttcaaaaat tatttgatgc
     3421 aaccttagac aatccagaac tcaccgcaac attagtgcct ttaggagatg gtattctcat
     3481 gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc gaatgatttt cagaaaaatt
     3541 taagaaaaaa tagtaaaata gatagagtaa cacttatctc aaaggagtag acatgaagaa
     3601 aaaattattg gcaggtgcca tcacactatt atcagtagca actttagcag cttgttcgaa
     3661 agggtcagaa ggtgcagacc ttatcagcat gaaaggggat gtcattacag aacatcaatt
     3721 ttatgagcaa gtgaaaagca acccttcagc ccaacaagtc ttgttaaata tgaccatcca
     3781 aaaagttttt gaaaaacaat atggctcaga gcttgatgat aaagaggttg atgatactat
     3841 tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt gtcttgtcac aagcaggtat
     3901 gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa ttagttgagt tggcagttaa
     3961 gaaggtagca gaagctgaat tgacagatga agcctataag aaagcctttg atgagtacac
     4021 tccagatgta acggctcaaa tcatccgtct taataatgaa gataaggcca aagaagttct
     4081 cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta gccaaagata attcaactga
     4141 tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat tctgcttcaa cagaagtacc
     4201 tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat ggtgtttctg atgtgattac
     4261 agcaactggc acacaagcct acagtagcca atattacatt gtaaaactca ctaagaaaac
     4321 agaaaaatca tctaatattg atgactacaa agaaaaatta aaaactgtta tcttgactca
     4381 aaaacaaaat gattcaacat ttgttcaaag cattatcgga aaagaattgc aagcagccaa
     4441 tatcaaggtt aaggaccaag ccttccaaaa tatctttacc caatatatcg gtggtggaga
     4501 ttcaagctca agcagtagta catcaaacga atagtccaaa tcaatgagtc agggaaaaaa
     4561 ctcgacttca ggaaaaaatg aagcaaacat tcccacaata aaacgcatag tacaaggttt
     4621 gtactgcccc ccaaaaagtt agacaattaa tttatccgaa ggatttagtt ctgtattgca
     4681 cagagctaag tccttttagt tttatcttaa ttctcttatt gttgtaataa tcaatatagt
     4741 ctataatggc t
//
LOCUS       DBDESG010000089         4698 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.089, whole genome shotgun sequence.
ACCESSION   DBDESG010000089 DBDESG010000000
VERSION     DBDESG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.089"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1288)
                     /gene="hisS"
                     /locus_tag="ACKB38_002080"
     CDS             complement(<1..1288)
                     /gene="hisS"
                     /locus_tag="ACKB38_002080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGL7980777.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="ACKB38_002081"
     CDS             complement(1366..2613)
                     /locus_tag="ACKB38_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGL7980778.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="ACKB38_002082"
                     /pseudo
     CDS             complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="ACKB38_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3778..4119
                     /locus_tag="ACKB38_002083"
     CDS             3778..4119
                     /locus_tag="ACKB38_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGL7980779.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4183..4392
                     /locus_tag="ACKB38_002084"
     CDS             4183..4392
                     /locus_tag="ACKB38_002084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980780.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSSKHCFEVVDRTDEVS
                     PKHCFEVVDRTDEVSNHTYGKATLT"
     gene            complement(4418..>4698)
                     /locus_tag="ACKB38_002085"
     CDS             complement(4418..>4698)
                     /locus_tag="ACKB38_002085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGL7980781.1"
                     /translation="GTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQDTKEISMAVSE
                     EELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGKK"
BASE COUNT         1474 a          985 c          941 g         1298 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgggtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aaggatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
      421 catcgtagcg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaaaagttta ttttccacat
     1021 aggaacggac aacgggtgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttgtag cgtttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgactg atactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
     2341 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccagg ttgaaaatcg aaacaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caacagttct atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgggagttt
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacgg acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcagatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctcttcata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctt gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgactctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag cagaccaatc acttttaaag
     4141 atgaaaaaag caagccgaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgctcaaaa cactgttttg aagttatgga tagaactgac
     4261 gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagcccaaaa
     4321 cactgttttg aggttgtgga tagaactgac gaagtcagta accataccta cggcaaggcg
     4381 acgttgacgt gatttgaaga gattttcgag tatgagttta ttttttacct gacttgtcca
     4441 tattccagaa gtctgtcacg gctccgcgtg aagcagatga tacgatgtgg gcatatttac
     4501 cgaggacacc acggctgtaa agtggtggca aggttgtttc tgccttgcgt ttttcaagtt
     4561 cttcttcgga tacggccata gaaatttctt tggtatcttg gtcaaccgta acgatatcgc
     4621 cggtacggag ataggcaatt ggtccaccat cctgagcttc aggagcgata tgtccaacaa
     4681 ccagaccata agtaccac
//
LOCUS       DBDESG010000090         4491 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.090, whole genome shotgun sequence.
ACCESSION   DBDESG010000090 DBDESG010000000
VERSION     DBDESG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.090"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1418)
                     /locus_tag="ACKB38_002086"
     CDS             complement(<1..1418)
                     /locus_tag="ACKB38_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142291904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HGL7980782.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVLRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGAVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNDTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASTSISASSTVVGSQTAAATEATAKKVEEDRKKPASDYVASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKNAAVFSGNTIVNGAPAINASLNIAKSETKVYTGEGVDSVYRVPI
                     YYKLKVTNDGSKLTFTYTVTYVNPKTNDLGNISSMRPGYSIYNSGTSTQTMLTLGSDL
                     GKPSGVKNYITDKNGRQVLSYNTSTMTTQGSGYTWGNGAQMNGFFAKKGYGLTSSWTV
                     PITGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTTSQSLSQSKSLSVSASQSASA
                     SASTSASASASTSASASASTSAS"
     gene            complement(1662..2123)
                     /locus_tag="ACKB38_002087"
     CDS             complement(1662..2123)
                     /locus_tag="ACKB38_002087"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGL7980783.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     DRYLYRPYAGAPRGEKVYGKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2090..2191)
                     /locus_tag="ACKB38_002088"
     CDS             complement(2090..2191)
                     /locus_tag="ACKB38_002088"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980784.1"
                     /translation="MKGGHRHVEAAKVFDVGVRTLFTWEKKDVNKGT"
     gene            2500..2880
                     /locus_tag="ACKB38_002089"
     CDS             2500..2880
                     /locus_tag="ACKB38_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGL7980785.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            2979..3200
                     /locus_tag="ACKB38_002090"
     CDS             2979..3200
                     /locus_tag="ACKB38_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGL7980786.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3217..3531
                     /gene="trxA"
                     /locus_tag="ACKB38_002091"
     CDS             3217..3531
                     /gene="trxA"
                     /locus_tag="ACKB38_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGL7980787.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            3837..4370
                     /gene="queF"
                     /locus_tag="ACKB38_002092"
     CDS             3837..4370
                     /gene="queF"
                     /locus_tag="ACKB38_002092"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGL7980788.1"
                     /translation="MKRQLALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         1358 a          932 c          846 g         1355 t
ORIGIN      
        1 gacgcactgg ttgacgctga agccgaggca ctggttgatg ctgaagccga cgcacttgtt
       61 gatgctgaag ctgaggcgct ttgactagca cttactgaga gtgacttaga ctgtgaaagt
      121 gactgactgg tcgtgctaga ttcaactacc tttcctccac cattgaagta gttaattcca
      181 attctatctg ttctagcagc gtaaggggta aatgtaaagg atgtatccgt tccagtaatt
      241 ggtacagtcc aagatgatgt taatccatat cctttcttag caaagaaacc attcatttgg
      301 gcaccatttc cccaagtata cccactaccc tgcgtcgtca ttgtagatgt attataggat
      361 agaacctgtc taccattttt gtcagtaatg tagttcttta cacctgaagg tttaccaaga
      421 tcactgccaa gggttaacat tgtttgtgtt gaagtacctg aattatagat agaatatcca
      481 ggacgcatac ttgatatatt accaagatca tttgttttag gattcacata cgtaaccgta
      541 taggtaaagg tcaattttga accatcattt gtcactttca atttatagta aattggaaca
      601 cgatataccg aatctacacc ttcacctgta taaacttttg tctcactttt agcaatgttt
      661 agacttgcat taattgcagg ggcgccattt acaatcgtat tgccagaaaa aacagcagca
      721 ttttttatag aagccagcaa ttgctcgatg gaatccactg aacgcttgcg tcgcttagca
      781 taagattgga gattgacatt tgtaactgat gctacataat cactagctgg tttcttacga
      841 tcttcttcga ccttcttagc agttgcttct gtagcggcag ctgtttgtga acctaccaca
      901 gtagatgatg cagaaatact tgtcgaagcc gatgtacttg ctgattctga tgcacttgta
      961 cttgctgagg tcgaagcgct ggttgaggct gactcagatg cagatgtact tgccgactct
     1021 gaagctgaca aactagttga actcgctgaa ttacttgtag atatcgtacc tagaactact
     1081 gtatcatttg ttgccaaagc atccgttttc tctacagttt tttcaagtac tgctgactca
     1141 tttgtaaata ccttcgtttg agttgcaacg gttccactta tgacagctcc cgcagcaact
     1201 atccccttga ggatatcaag cccagtaatt gaatgactta ctttatcttc taccgtttcg
     1261 gtcatgacct gagtagtatc aacaccacct cgcaagacct taaacaagcc aaaaagagaa
     1321 gtcgaggccc gtagccaatg cttccctgat ttgattaatt tgaaccgagt tactcggtct
     1381 gtttctctat attcaccctt ctgacgtcta aaaaatattt tgaaatttcc tttcgagtat
     1441 tattttcatg tatggatata aataaattac catactataa ttatagaata gggaacccaa
     1501 aatagcaaga aacttgaggt atttttgcta aaaaaaaaaa gcattttact attttgtata
     1561 tatatatata tattaagata agttttttat aaagacacgc aaatgagtat aaatcagcta
     1621 atttttggtt tatattccta tttttatgca taattattct ttcattctct aatttgtgaa
     1681 tcaatatatc tgattagaaa actaccattg agtttttgct ccacctcatt agtccgctca
     1741 aaacgccgtc cgctaatctt gccatagact ttctcccctc taggagcccc tgcataagga
     1801 cgatagaggt agcggtcgat tcccgtttcg tcaatatata ggactggtaa atcttttagg
     1861 ttatccaaaa tatcaagaaa ctcagaaact ttctcaggct tttgttccct aaaactggtc
     1921 gtctttttta aaatgacctt aatctgcttt aaaactgccc atacggaggg caaagcacaa
     1981 tcaaaacggg ccgcaatttc ccgtaaaaaa gcgtctggat gagcctctac aaaggctttc
     2041 aattcttcta aagggatctt acgctttttg acgactcgct ttttccgctc taagttccct
     2101 tgtttacgtc tttcttttcc cacgtgaaga gagttctgac gccgacatca aaaactttgg
     2161 ccgcctcgac atgtctgtgc cctcctttaa tggaatctaa tgctccttgt ttaaaatcag
     2221 tactatatgc atagctttat tagaccatgc ttattttaaa ctaagcaact atagatagag
     2281 aaatgaaata aaatctgaat tcgaatgtga taaggagtta gaaattgaaa caataggatt
     2341 cccccattat tcacacaaaa ataaaaccac taaaaaacag agcttgccag cattcttgaa
     2401 aagtagtata attattgcat gcgcaatcat cttgtgacgc agagactgcc cgttaatgga
     2461 ctttcttcta tttatgactc taaaaagaaa ggagacacta tgacctattt ggaaaaatgg
     2521 tttgacttca atcggcgtca gaaagaaatt gaaagtctct tggaagagac cattgcccaa
     2581 cagagcgagc aaagtctgac cttgaaagag ttctacctgc tctactatct ggacttggct
     2641 gaagaaaaat ctctacgcca gattgacctg ccagataagc ttcatctgag cccgagcgca
     2701 gtttctagga tggtggctcg cttggaagct aaaaattgcg gtctactcag tcgcatgtgt
     2761 tgccatcaag atagacgctc tagttttatc tgcctgacaa atgatgggca aaagacactg
     2821 gcctcgctac aaaagactgt cgaagaaagc ttggaagcag gtttggattt cttgatttaa
     2881 gatgatttta aaaaaagttg cgtgcgcaat catttttctt gacattctct tttataagga
     2941 gtaaaataaa gttagcatta aacaaaggag ttttaaacat gattgaaatt acctatatag
     3001 atgccagcaa gaacgaaaga actgtgactt tcgagtctta tgaagacttt gaacgttcgc
     3061 aacaagcttg ccttatcggc gtcgcagact actaccctgt ccaaaaatta acttacaagg
     3121 gtcataattt ggactaccat gggacttatg gagatatctt cttctatctc atgaaacaag
     3181 atttaagcca atataactaa aaaaaggaga aatacaatgg caaaagcaat tacagatgca
     3241 acattcgaac aagaaacaaa agacggtttg gtcttagtag acttctgggc aacttggtgt
     3301 ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt ctgaagaact ttcagaagat
     3361 gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa acacagctcg tgcttttgga
     3421 atcatgtcta ttccaactct tctcttcaaa aaagacggcc aagttgtcaa acaagttgca
     3481 ggtgttcaca cagcagaaca aatcaaggcc atcattgctg aattgagcta atcaaacgag
     3541 agaccaagta ctttctttgg tctctttttt cttgcccttt gccatttttc aaaaaatatg
     3601 ctagactata gtagattgaa actagaatag tacacctcta cttctaaaac attgttagaa
     3661 atcgatttga ctgtcctgat cgatttgtcc tgttcttatt tcattttact atataagtag
     3721 aatattacga tgtttgggat atgccatcgc taaaaaaacc tcgatttggt attttttagc
     3781 tccctaatgg gagctttttg cgtgctctga acatttccca ttttggaagg agtactatga
     3841 aacgtcaatt agccttggtc gtctttagtg gtggtcaaga ttcaacaacc tgccttttct
     3901 gggtcatgca acactatgaa acagtcgaag ctgtcacctt tgcctacggc caacgtcatc
     3961 acctcgaaat tcaaattact agagaaatcg ctaaggaaca gggcattcgt caccatatcc
     4021 tcgatatgtc tctgctggga caaatcactg ctcagccaga ctttgcgacg attcatattt
     4081 cctacattcc tgacaagctc tgtgtcgagt caaaatccct caaactatat ctatttagct
     4141 accgaaacca cggagatttc cacgaaaact gtatcaacac catcgggaaa gacttggtca
     4201 acttgctaga ccctcgctat ttagaagtct ggggaaaatt cactccgcgc ggtggcattt
     4261 caatcgaccc ctactacaac tacggtaagc aaggaactaa gtatgagggc ttggcagaac
     4321 aacgcctctt ccaacacgac ctttatccag agaaaattga caaccgctaa agtcatactc
     4381 aatgaaaatc aaagagcaaa ctaggaagtt agccgcaggt tgctcaaagc actgttttga
     4441 ggttgtggat agaactgacg aagtcagatc aaaacactgt tttgaggttg t
//
LOCUS       DBDESG010000091         4257 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.091, whole genome shotgun sequence.
ACCESSION   DBDESG010000091 DBDESG010000000
VERSION     DBDESG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.091"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(36..209)
                     /locus_tag="ACKB38_002093"
     CDS             complement(36..209)
                     /locus_tag="ACKB38_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HGL7980789.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(368..1975)
                     /locus_tag="ACKB38_002094"
     CDS             complement(368..1975)
                     /locus_tag="ACKB38_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGL7980790.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKFYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            2128..3612
                     /locus_tag="ACKB38_002095"
     CDS             2128..3612
                     /locus_tag="ACKB38_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGL7980791.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3707..4189
                     /locus_tag="ACKB38_002096"
     CDS             3707..4189
                     /locus_tag="ACKB38_002096"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGL7980792.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1228 a          978 c          813 g         1238 t
ORIGIN      
        1 taccttttat gaggctcttt tgtcttgttc ttgtttcaat tgactatatt atcagcgtaa
       61 tagtctaata gatagttaaa tgtagttgga tttccactaa aggttgatga gtaagttttt
      121 gtatctgaac ctgattggcc gcaagcagct aaaagcaaag cagatgcaaa agtcaggcct
      181 gcactaagga cacgcttctt tatgttcatc ttctttctcc ttaatagtgg gaatttgtaa
      241 agttaattga atttcaagaa tgaaagttta taaactttgg ttataaaaaa caaaggattt
      301 ctgtctttta tacagtcctc ccttgttttt atacgatttc aattttaaat ttttctgcaa
      361 aaaatattta tagtaattcc acacagaaag catcccatgg agccaagacc tgtttttcaa
      421 gtacttcttt agccgcagtg ttttcaatca agacagattt gacttttcct tctactgtca
      481 agtcttgctc ttcattggac aagttagcca caactaggaa gcgacggtcg ccatccttac
      541 gtatataagc aaagacctta tcagccgtat caagcaattc aaagtcagct cgaactagcc
      601 agctattctc cttgcgaatt tggaccagtt tctgataggt atagaaaata gaatctggat
      661 ttgccagcgc ttcttggaca ttgatcatct cgtaatttgg attaaccgcc aaccaaggtt
      721 gacctgttga gaaaccagcg tttttgctct cgtcccattg cataggggta cgggcattgt
      781 cacgtccaat aacacggata ctgtccatga tttcttcaat cggaacacct ttttcaagag
      841 cctcacgcgc atagttgaga gattcaatat cttctacttg atccagtgtt tcaaacggat
      901 agttggtcat cccaatctcc tcaccttggt agatataagg agttcctctc atgagatgaa
      961 gtaagattgc aaaggctttg gcagattttt cgcggtattc ttggtcattt ccccagattg
     1021 agacaatacg agggaggtca tggttgttcc agaagaggga attccagccg tcctcaactc
     1081 ctaactctgt ctgccatttg ttgaagattt cttttaactt agcgatattc agctcttttt
     1141 gatagtgcca tttaggctga ccttcctgat actgaagacc gatatgttca aactggaaga
     1201 ccatagacaa ttcttgcccc tttggatcag agtagaactt ggcaatctct ggagttgctc
     1261 cccaagtctc ccctactgtc aagagatcct tatctccaaa ggtcgcctga ttcatttcct
     1321 tgagataggg atggagcata ggaccattat tgactacctt ctcgtcagga attttgccaa
     1381 tcatgtcaat aacatccata cggaaaccac caataccttt atcaatccag aagttcatca
     1441 tctcataaat tttctggcga agtttttcat tttcccagtt gagatccggc tgtttcttgc
     1501 tgaaaaagtg gagatagtat tgacctgact tttcatcgta ttcccaagca gacccactaa
     1561 agatagaatc taggtcattg ggttcatcgc gccagatata gtagtctcgc tcagggctgt
     1621 cagtattttc acaggcttcg acaaaccaag catgttcatc tgaggtatga ttgaccacca
     1681 agtccatgat gatacgaatg tcacgcttct tagcttctgc aatcagctga tccatgtcct
     1741 ccatggttcc aaaaatagcc gcaatcgctt gataatcagc aatatcatag ccattatcat
     1801 ccatagggct gtcataaacg ggagaaagcc aaattgctgt gattcctagc ttagctagat
     1861 agtccaactt actggtaata cctggcaaat caccaactcc atctccatta ctatccataa
     1921 aactctttgg atagacttga tagactacgg cattgtgcca ccatttttct tgcatctgtt
     1981 tacctcatta ttttcataat ctatagtcta tgatagcact ttttaaaatt ttgtgcaagc
     2041 gtttgcttaa tctttttaat ttctttttta gctccgtagt ttcctaactt ccaatttttt
     2101 attataatag agtccagagg taaaaaaatg aaaaaacaag cttttagttc tgaacaatat
     2161 ttgaatttac aacgcgacca tattttggag cgcattaacc aatttgacgg caagctctac
     2221 ttagagtttg gcggtaaaat gttagaagat ttccacgctg ctcgtgtcct tcctggttat
     2281 gaacctgaca acaaaatcaa gctcttgcaa gaattgaaag agcaggttga ggttgtgatt
     2341 gccattaatg ctagcaacat tgaacattcc aaagcacgtg gcgacttagg catttcttat
     2401 gaccaagaag ttcttcgttt gattgataaa ttcaatgaat tagggatttt tgttggttcc
     2461 gttgtcatta cacaatacgc tggccaaccc gctgcagatg ccttccgcaa tcaacttgag
     2521 aaaaacggaa ttgattctta tcttcattat ccaatcaaag gatatccgac ggatatggat
     2581 cacatcattt ccccagaagg catgggcaaa aacgactaca tcaaaaccag tcgcaacttg
     2641 attgtcgtaa ccgctcctgg acctggttct ggaaaattgg caacgtgtat gtccaatatg
     2701 taccacgacc aaatcaatgg catcaaatct ggctacgcta aatttgaaac cttccctgtt
     2761 tggaatcttc cccttcatca tccagttaac ttggcttatg aagctgccac agctgacctt
     2821 gatgatgtca acatgattga ccccttccat cttcaaacct atggagaaac cactgtcaac
     2881 tacaaccgtg atatcgaaat cttcccagtg ctcaaacgca tgttggaacg tattctcgga
     2941 aaatcaccat acgcttcacc gacagatatg ggtgtcaaca tggttggttt cgctattaca
     3001 gatgacgagg ccgctgtcga agcttctaaa caagaaatca tccgccgtta ctatcaaaca
     3061 gttcttgact tcaaagctga aaaagttggc gaagctgccg tcaagaaaat tgagttgctc
     3121 atgaacgacc tcggtatcac acctgcagac cgtaaggttg ctgtcgttgc gcgccaaaaa
     3181 gcagaagaaa ctggtggacc agccctagcc tttgaattgc caaatgggga aatcgtcact
     3241 ggtaagaact cagaactctt tggtcctaca gccgctgcct tgatcaacgc catcaaaaaa
     3301 tcagctgaca tcgctaaaga agtaaaacta atcgagcctg aagttgttaa gccaatccaa
     3361 ggtcttaaaa tcgatcatct cggtagccgc aatccacgcc ttcattcaaa tgaaatcctg
     3421 attgcacttg ctatcacagc tacagaaaat cctgatgctg cccgcgctat ggaagaactc
     3481 ggcaacctca aaggaagcga agcccactca accatcatct tgactgatga agacaagaat
     3541 gtccttcgta aactgggtat caacgtaacc tttgacccat actaccaata cgaccgctta
     3601 tatcgtaagt aaagatttca acctctccag cctcggagag gttttctctc tgtctcagga
     3661 gcaaccttta tgatataatg agagaagaaa actgaaagga tttaccatgt caaaagaagt
     3721 tattgtcgaa agttttgaac ttgaccacac cattgtcaaa gcaccctatg ttcgcttgat
     3781 tggggaagaa acaggaccaa aaggagacat catctccaat tatgatattc gcttggtgca
     3841 accaaacgaa gactctatcc ctactgccgg ccttcacact atcgagcacc tcttagccaa
     3901 actcatccgt acccgcattg acggcatgat tgactgttca ccatttggtt gccgcacagg
     3961 cttccacatg attatgtggg gacgtcacac cagtgctaaa atcgcagctg ttatcaagga
     4021 ttcgctcaag gaaatcgctg aaactactac ttgggaagat gtcccaggga caaccatcga
     4081 atcttgcgga aactacaagg accacagcct cttctctgct aaagaatggg caaaactcat
     4141 cttggaacaa gggatttcag atgatgcctt tgaacgtcat gtgatttaaa atcgagtagg
     4201 ggataatctc tagcccctct cacaccaccg tacgtaccat tcggcatacg gcggttc
//
LOCUS       DBDESG010000092         4081 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.092, whole genome shotgun sequence.
ACCESSION   DBDESG010000092 DBDESG010000000
VERSION     DBDESG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.092"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..2002
                     /gene="leuS"
                     /locus_tag="ACKB38_002097"
     CDS             <1..2002
                     /gene="leuS"
                     /locus_tag="ACKB38_002097"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGL7980793.1"
                     /translation="NEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELD
                     WSESIKDMQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVD
                     AITSSEQAEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADY
                     VLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLD
                     GLNKEDAIAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVP
                     ETELPLVLPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYY
                     LRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPIKE
                     PFQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKN
                     VVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILA
                     ENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLI
                     APFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAK
                     DLSREELQEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            2197..2891
                     /locus_tag="ACKB38_002098"
                     /pseudo
     CDS             2197..2891
                     /locus_tag="ACKB38_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2903..3319
                     /locus_tag="ACKB38_002099"
     CDS             2903..3319
                     /locus_tag="ACKB38_002099"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGL7980794.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQTEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKEALGNFKEAYQVQ
                     LATEETEKNVGFYRSMGFEILSTYDCTGMIWINREK"
BASE COUNT         1213 a          772 c          987 g         1109 t
ORIGIN      
        1 caatgaagaa gtgcttcctg acggaacttc tgagcgtgga ggctatccag ttgtccgcaa
       61 accaatgcgc caatggatgc tcaaaatcac ggcttacgca gagcgcttgc tcaatgactt
      121 agatgaacta gattggtcag agtctatcaa ggatatgcaa cgcaactgga ttggtaaatc
      181 aactggtgcc aatgtaactt tcaaagtaaa aggaacagac aaggaattta cagtctttac
      241 tactcgtccg gacacacttt tcggtgcgac tttcactgtc ttggctcctg aacatgaatt
      301 agtagacgct atcacaagtt cagagcaagc agaagctgta gcagactata aacaccaagc
      361 cagccttaag tctgacttgg ctcgtacaga ccttgctaaa gaaaaaacag gggtttggac
      421 tggtgcttat gccatcaacc ctgtcaatgg taaggaaatg ccaatctgga ttgcagacta
      481 tgtccttgct agttatggaa caggtgcggt tatggctgtg cctgcccacg accaacgtga
      541 ctgggaattt gccaaacaat ttgaccttcc aatcgtcgaa gtacttgaag gtggaaatgt
      601 cgaagaagct gcctacacag aggatggcct gcatgtcaat tcagacttcc tagatggatt
      661 gaacaaagaa gacgctattg ccaagattgt ggcttggttg gaagaaaaag gctgtggtca
      721 ggagaaggtt acctaccgtc tccgcgactg gctctttagc cgtcaacgtt actggggtga
      781 gccaattcca atcattcatt gggaagatgg aacttcaaca gctgttcctg aaactgaatt
      841 gccgcttgtc ttgcctgtaa ccaaggatat ccgtccttca ggtactggtg aaagtccact
      901 agctaacttg acagattggc ttgaagtgac tcgtgaagat ggtgtcaaag gtcgtcgtga
      961 aaccaacact atgccacaat gggctggttc aagctggtac tacctccgct atattgaccc
     1021 gcacaatact gagaaattgg ctgatgagga cctcctcaaa caatggttgc cagtagatat
     1081 ctacgtgggt ggtgcggaac atgctgtact tcacttgctt tatgctcgtt tctggcataa
     1141 attcctctat gacctcggtg ttgttccgat taaggaacca ttccaaaaac tctttaacca
     1201 agggatgatt ttgggaacaa gctaccgtga ccaccgtggt gctcttgtgg caaccgacaa
     1261 ggttgaaaaa cgtgatggtt ccttcttcca tgtagaaaca ggggaagagt tggagcaagc
     1321 gccagccaag atgtctaaat cgctcaagaa cgttgttaac ccagacgatg tggtggaaca
     1381 atacggtgcc gatacccttc gtgtttatga aatgtttatg ggaccactcg atgcttcgat
     1441 tgcttggtca gaagaaggtt tggaaggaag ccgtaagttc cttgaccgag tttaccgttt
     1501 gattacaagt aaagaaatcc ttgcggaaaa caatggtgct cttgacaagg tttacaacga
     1561 aacagtcaaa gctgttactg agcaaattga gtctctcaaa ttcaacacag ctattgccca
     1621 acttatggtc tttgtcaatg ctgctaacaa ggaagataag ctttatgttg actatgccaa
     1681 aggctttatt caattgattg caccatttgc acctcacttg gcagaagaac tctggcaaac
     1741 agtcgcagaa acaggtgagt caatctctta tgtagcttgg ccaacttggg acgaaagcaa
     1801 attggttgaa gatgaaattg aaattgtcgt ccaaatcaaa ggaaaagttc gtgccaaact
     1861 catggttgct aaagatctat cacgtgaaga attacaagaa atcgctttag ctgatgaaaa
     1921 agtcaaagca gaaattgacg gtaaggaaat cgtgaaagta attgcggtac cgaataaact
     1981 cgttaatatc gtcgttaaat aacgagttta ttagctctat ctgccacctt caatagtcca
     2041 ctggactatt gaagccaact aaattagtta acattgttgt gaaataagat aggagtcctt
     2101 cagagtagaa tctggaggat tttttgaatc ttcttatgaa agtatgatat actatgggca
     2161 actataaagt ttgaaaagtg aaataaggag aataagatgc cagtaaatga atatggtcaa
     2221 atgattgggg agtcaatgga agcttatact ccaggtgaat tgccttcttt tgatttctta
     2281 gaagggcgtt atgctaggat agaggctctt tcagtggaaa agcatgcgga ggatttatta
     2341 gctgtttatg gccctgatac gcctcgggag atgtggacct acctctttca ggagtcagta
     2401 gcagacatgg agaactggtc agccttttaa atcagatgtt ggctcgtaag gaccgttttt
     2461 attatgcaat catagacaag gcaactggta aggctttggg aactttttcc ctcatgcgaa
     2521 ttgatcagaa taaccgagta atagaagtgg gagctgtcac tttttctcca gagctcaggg
     2581 ggacacggat aggaacagaa gcccagtatc tcttggcttg ctatgtcttt gaggagctta
     2641 actatcgtcg ctatgagtgg aaatgcgatg ctcttaacct gccatccaga cgagcagcgg
     2701 aacgtttggg atttatttat gaaggaacct tccgtcaggc agtggtttat aaggggcgta
     2761 caagagatac ggattggttg tctatgattg ataaggactg gcctcaagtc aaagctcgat
     2821 tggaaatatg gttgcgtcct gaaaactttg ataaaaatgg acgacagcac aagagcttga
     2881 gagaacttta agaggtgttg agatgattac tattaaaaag caagaaattg tcaagctaga
     2941 ggatgttttg catctctatc aggctgtcgg ttggacaaac tatacccatc aaacagagat
     3001 gctggagcag gccttatctc attcattagt aatttatctg gcacttgatg gtgatgctgt
     3061 ggtgggcttg attcgtttgg ttggagatgg tttttcatca gtttttgtac aggatttgat
     3121 tgttttgcct agctatcagc gtcaagggat tggtagctcc ttgatgaaag aggctttagg
     3181 aaattttaaa gaggcctatc aagtccagct ggcgacagaa gagacagaaa aaaacgtggg
     3241 attttatcgt tctatgggct ttgaaatctt atccacctat gactgtacag gaatgatttg
     3301 gataaacaga gaaaaataaa aaaacttgtt tgttcttaag caaagtttaa ggatggtcta
     3361 gtatcatata gtcattaaat aaagacctcc taactttatt taataaaatc ctaaactttt
     3421 ttcatcacaa tctcctaatg aagccaccca atcaggtggc ttttttgcgg tacgacgggc
     3481 atgtcgtata tctgaggtgt aagtcctcgg tgggcacctg ctaccggtga acccaatagc
     3541 gattcccaag cctgactatc gtgaggtagc agggagagga agggatagcg aaatcgtggc
     3601 tctacgaaca ggaacgtgat agtaaggcgt atatagcgga taaggaggct tcaaactcta
     3661 aagtccaaaa aggtagtcgt aacctatatg tgtaaatcac gagagtaatt gaattcggac
     3721 taaggtttgt gtgaaaaaga taaatctttc tagagtctaa agactctgcg tcagatttcc
     3781 tattttcact gtaacctttt aacgtcctca tatcttgtat aaacgaggaa agatgtacga
     3841 cttatcccgt gaggtttcat gagcgctgaa agcgtagtaa caacgaatca tgagaagtca
     3901 gccgagccca tagtagtgag gaaacttccg taatggaagt ggagcgaagg ggtgaatact
     3961 caaacagtct ggggagagac tgtttgaggt ctgtcgctag aaagagaaaa cgacagatcg
     4021 aagtaatcct acttcacttg tgtctgtaaa atgagtggtc tgatagaact ggactttgac
     4081 a
//
LOCUS       DBDESG010000093         3911 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.093, whole genome shotgun sequence.
ACCESSION   DBDESG010000093 DBDESG010000000
VERSION     DBDESG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.093"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            69..491
                     /locus_tag="ACKB38_002100"
     CDS             69..491
                     /locus_tag="ACKB38_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGL7980795.1"
                     /translation="MNTDYIARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGE
                     NHQEALKHELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKP
                     LENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="ACKB38_002101"
     CDS             656..955
                     /locus_tag="ACKB38_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGL7980796.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1064..2401)
                     /locus_tag="ACKB38_002102"
                     /pseudo
     CDS             complement(1064..2401)
                     /locus_tag="ACKB38_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2642..2929
                     /gene="tnpB"
                     /locus_tag="ACKB38_002103"
     CDS             2642..2929
                     /gene="tnpB"
                     /locus_tag="ACKB38_002103"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGL7980797.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(3120..3911)
                     /locus_tag="ACKB38_002104"
                     /pseudo
     CDS             complement(3120..3911)
                     /locus_tag="ACKB38_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612110.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1186 a          766 c          666 g         1293 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagt gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcatgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttcaataata aaacgcatag
     1021 tagcaagagt tttgtactga ccccaaaaag ttagacaatt aatttatcca aaggatttag
     1081 ttctgtactg cacaggacta agtcctttta gttttacctt aattcgtttg ttgttgtagt
     1141 aatcaatata gtctataatg gcttgttcca attggttaag tgatttaaat gttttctcat
     1201 agccataaaa catttcggat tttaaaatgc caaagaaaga ttccatcata ccgttgtctt
     1261 ggctgttgcc cttgcgtgac atagatgctt gaattccctt actctctagg aaccgatgat
     1321 aagaatcgtg ttgatattgc cagccttggt cactatggag aatcgtattc tcgtagtgct
     1381 tctctttgaa tgcctgttct aacattgttt gtacttgttc taagttgggt gaagtcgaaa
     1441 gattaaaagc aataatttcg ctattaaagc catctaaaac tggtgataag taaagctttt
     1501 gagtacttgc tggaatggca aattctgtca catctgtgta gcacttttcc attgttttag
     1561 agccttcaaa ttggccttga atgagattct ctgccttctt accaacgtct cctttatttt
     1621 cgtttctgtc gcattttagc ttgtaaattg agtactttca tcaagccttg aactctttta
     1681 tgatttacca gataaccacg atttcttagt tctaaataaa tccgacgata accataattt
     1741 cccttgtgtt cgataaaaat ggattgaatt tcagctttaa gctcttggtc cttatctggt
     1801 ttgtctagct gtttcaagtg atagtagtag gtccaacgag ctagtttaat ggcttttaga
     1861 agaagatcta acgaaaactc agtcattaat tcttgaacaa tttctgtctt tcttctttct
     1921 ctttttcctc cttccatcgg agttctctta acttttttag gatagcattc tccgctctca
     1981 ggtactcatt ttctgcttga agacgttcta attctgttct ctcttcaggt ctcttttttg
     2041 gcttacgtcc cattttaggt actctccctc ttgttttctc aacaatagta tacccgtttt
     2101 ttctgtattg tgctagccag ttaagaagta tcgtacgact tgggagaccg tattcaagag
     2161 aaactctatc tttagttcag ccttcatgta agactttatt aatcatttct tgttttaaat
     2221 caggaaaata gtaacgattt tttccttttt tgacgaactc tattccgtaa cgatcaatca
     2281 atttaatcat atacctaaga ttagaattat ttatcccaaa tttatttgaa agcttctcta
     2341 agctatatcc ttgttttcta agttcataga tctgaacttt atcatcataa cttaatttca
     2401 taacaaaaac accccaaaag ttagattttt tctgtctaac ttttggggca gtacatttca
     2461 atacctgata ctatgcgttt ttatcatttt gaaatctact gctaatcaat gaagaagcaa
     2521 agatcaaact aggcagctag ttacaagtta cgtaagcgcg tcataacaag gtatctatcc
     2581 taattcccca acttccagta tactagataa aaaataatta tcggaggaaa ggtattgtac
     2641 tatgataatt caactaagtg atttaggtca agttcacctt gtttgtggca agacagatat
     2701 gaggcaggga atagactcat tagcctatgt agttaaaacc cactttgaat tggattcttt
     2761 ctccggtcaa gtctttctct tttgtggtgg acgtaaagac cgctttaaag ccctttactg
     2821 ggatggtcaa ggattttggc tactatataa acgctttgag aacggaaaac tgacttggct
     2881 aagtacagaa aaagatgtca aagctctcgc acctgaacaa gtagactagc ttatgaaggg
     2941 cttttctatc actccaaaaa tatagtagat tgaaactaga atagtacacc tctgcttcta
     3001 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
     3061 tactataaat ccatcagaaa gtcgtgattt ctattgaaat gaggactttc tttttatact
     3121 catctgtttt caaaaagcat tctagtccat ctccgattaa cgatggactt tatcacctcc
     3181 ttctccaatc cttgtataac atcttggagt tgattcatga catcttccaa agtttgaaag
     3241 gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat ggggttcatc
     3301 tctggtgtgt atggaggaat aaaggtaaaa ccaatattag tcggaatctt taaggtactt
     3361 gatttatgcc atatagcatt gtccataacg agtaaaagat aatcatctgg ataagcttgt
     3421 gaaagctctt ctaaaaaggc gttcatccac tcagtattac atctaccagc tattaagaaa
     3481 aatgattcgc ctgtatgggc atcaacagct ccataacaat agcgaaattc tcgtatatag
     3541 tgactatgga tatgtggacc tactcctatt ggagcccaac aagatcccag tttactgatt
     3601 ctaccgaaac cagcctcatc ttggtacatc aagcgaacct tacgaaaacg tctactggtt
     3661 ttaaagcgct ttcttttctt cttgaattga gattttattt ttagacgcga caatggtttg
     3721 agcgtctgct ttcttaggat gttctggacg tggcataata tttcgccaac catggcactt
     3781 caacagttga tagaaggcat cacgtgtgta ggaacgacct aactcctttt tataagcctg
     3841 aaataaggca tcaattgtaa caaattctcc tgcctctgca gccttcaaat ggcgggcaag
     3901 aaagactttc t
//
LOCUS       DBDESG010000094         3836 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.094, whole genome shotgun sequence.
ACCESSION   DBDESG010000094 DBDESG010000000
VERSION     DBDESG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.094"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..326)
                     /locus_tag="ACKB38_002105"
     CDS             complement(<1..326)
                     /locus_tag="ACKB38_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGL7980798.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQ"
     gene            487..693
                     /locus_tag="ACKB38_002106"
     CDS             487..693
                     /locus_tag="ACKB38_002106"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980799.1"
                     /translation="MKLKKLLKDDTKVFEKSTFKFVEGYKIYLTESKESGIKQMDNVI
                     KYFEFIESKSIALYFQKRLNELID"
     gene            complement(1047..1580)
                     /locus_tag="ACKB38_002107"
     CDS             complement(1047..1580)
                     /locus_tag="ACKB38_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGL7980800.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1669..2445)
                     /gene="recX"
                     /locus_tag="ACKB38_002108"
     CDS             complement(1669..2445)
                     /gene="recX"
                     /locus_tag="ACKB38_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGL7980801.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2483..>3836
                     /gene="rlmD"
                     /locus_tag="ACKB38_002109"
     CDS             2483..>3836
                     /gene="rlmD"
                     /locus_tag="ACKB38_002109"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGL7980802.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1229 a          647 c          687 g         1273 t
ORIGIN      
        1 ttttggttga tgatacgagg gatttggtga tttttcttta ccaggggagt ctcagcaacc
       61 atcatttttg aacagtgata gcacttgaaa cgacgctttc taaggagaat tctagaaggc
      121 ataccagtcg tttcaagata aggaatttta gaaggttttt gaaagtcata tttcttcaat
      181 tggtttccgc actcagggca agatggggcg tcgtagtcca gtttggcgat gatttccttg
      241 tgtgtatcct tattgatgat gtctaaaatc tggatattag ggtctttaat atcgagcagt
      301 tttgtgataa aatgtaattg ttccatatga atctttctaa tgagttgttt tgtcgctttt
      361 cattataggt catatgggac tttttttcta caacaaaata ggctccataa tatctataag
      421 ggatttaccc actacaaata ttatagagcc gaaaattcac atctaatata tgcagactac
      481 tttgaaatga aattaaaaaa attattaaag gatgacacaa aagtttttga aaaatctaca
      541 ttcaaatttg tagaaggata taaaatatac ctgacagaat ctaaagaatc tggaattaaa
      601 caaatggaca atgtcataaa atattttgag tttattgaat ctaaaagtat tgctttatat
      661 tttcaaaaac gattaaatga gctgatagat taaatagcat tttctctgtt gagatattgt
      721 ttttaaaata ttgtactaaa tgattgatgc tatgtggaaa tacaaaaaaa tgtttttgat
      781 acgaagttga cctgtatttt ttatactaat cattttcgta ttttttgtat taaacgatat
      841 aagtttgttg taaacttaca aggaataaag acattaaaaa ataacagtat atctatttgt
      901 tttatatatt ttacgaattc tgcataaatc tctttctagt aatgtgttgt aactctgcta
      961 taatagattt attccttttt gtgtttacac aatttatttt atactaccaa aaaaggtcag
     1021 gattttgttc ctgacctttg acaactttac cgattcttta gttctacata gcgcttgtac
     1081 caaatgttta cataggcttc tgagaaagga ccacgtccat tgttaatcca atcaacaaga
     1141 attttgacat gttcttttaa aatatagtcc aagtcatcag aataattcat tttgcgtttg
     1201 tgacgctcgt actcttcaac gtccaagaga cgtttttccc catctgtaaa aattttaaca
     1261 tccaaatcgt aatcaatata cttcagtgct tcttcatcca gatagtaggg gctagccata
     1321 ttgcaatagt aagaagttcc attatcacga atcatggcaa tgatattaaa ccaatatttc
     1381 ttgtgaaagt aaacaatagc cggttctcga gtgacccaac gacgaccatc actttcggta
     1441 acaagtgtat gatcgttgac accaataatg gcgttttctg ttgtttttag taccatggtg
     1501 tcccgccaag ttcggtggag actcccatca tgcttataac tttgaattgt aataaagtcg
     1561 ccttcttttg gaagcttcat aactaaccaa ctttctacaa tttataagtt tatcatttac
     1621 tattgtacca taaaattacc caaaatctgt gaatttcact tggaaatatt aaagatattc
     1681 tctaagagcg cttgctatat ccgaaaaatc gtagcccttt cgtgctaaaa cttgagttaa
     1741 acgctgcttc agttcgtatc cttcatactt tcgggcatac ttagtatatt gcttatcaag
     1801 ttccttgaag atgagttcct gagtcgtttc ttcatcaact tgactatcca attcgtcaaa
     1861 ggcaatttta gcatcaaaat aagagaagcc cttgttagtc aagttctgga taatcttatc
     1921 ttgcagggca cgagctggaa gttttccctc atattttttc aatagtttat tggctacacg
     1981 ttgagcaact tccgaaaaat caaaatcatt caagatttct tctatagtag attttgaaat
     2041 tcctttttgt gctaatttct gagtcagtac ataaggtccc ttgtctcctg aaagttgatt
     2101 ggcattgatg atagcataag cgtactggct atcattaatc cacttctctt ctttaagatt
     2161 agcaatgact tgagaaacga tgttttcatt aatatcatat tttttcagat attctctgac
     2221 ctctttttca gtacgtgctt taaaggataa gtggtagagg gccagattct taccataaga
     2281 aaattgagca aagtcttgaa tctctttcaa ttcctcttcg cttatcacct tatctctcga
     2341 taacataaaa cgaacaattg tgtcttcggt gatatagcat ttgtcgccat tatcaagctc
     2401 catcagatag agtctttttt tcttttcaag ttttgtgatt ttcatagctc tattataact
     2461 caaaatgtga taagataggg gtatgaatct gaaagtgaaa caaaaaatac cattaaaaat
     2521 caagcgcatg ggaattaacg gtgagggaat cggcttttac caaaaaacat tagtctttgt
     2581 accaggagct ctcaaaggcg aagatatcta ttgtcagatt acttctatta gacgcaactt
     2641 tgttgaagca aaattactga aggtcaacaa gaagtctaaa tttcgaattg tgccatcttg
     2701 tactatttat aatgaatgcg gaggctgcca aatcatgcac ctgcattatg ataagcagct
     2761 ggagttcaag acggacttac ttcatcaagc gctgaaaaaa tttgctcctg caggatatga
     2821 aaattatgaa attcgtccaa ctattggaat gcaggaacca aaatattaca gagctaagtt
     2881 acaatttcag actcgaaaat ttaaaaatca ggtcaaggcg ggcttatatg cacaaaactc
     2941 tcactattta gtagagttga aagactgcct ggtacaagat aaggaaaccc aagtgattgc
     3001 taatcgctta gcagaattac ttacttatca ccagattcca atcacggatg agagaaaagt
     3061 tctaggtgtc cgtactatta tggtccgacg cgcgagaaag accggacagg ttcagattat
     3121 tattgttaca aaccgccagc ttaatttaac tcaattggta aaagagttgg ttaaagattt
     3181 cccagaagtt gtgacagtag ctgttaatac aaatacagct aaaaccagtg agatatatgg
     3241 tgaaaagaca gagattatct gggggcaaga gagtattcaa gaaggtgtac tcaattatga
     3301 attttcacta tcccctcgag ctttttatca actaaatcct gagcaaacag aagtcctcta
     3361 tagcgaagca gtaaaagcgc tggatgttga taaagaagac catttgattg acgcttattg
     3421 tggagttgga acgattggat ttgcctttgc aaagaaagta aaaacactca gaggtatgga
     3481 tattattcca gaagctattg aagatgccaa gcgaaatgct aaaagaatgg gatttgacaa
     3541 tactcattat gaagctggaa cggcagaaga gattattcct cgttggtaca aggaaggcta
     3601 ccgagcagat gctttgattg ttgacccacc acgtacaggt ctggatgata agttattaga
     3661 tactattctt acttatgtac cagaaaaaat ggtttatatt tcttgtaatg tttcgacctt
     3721 ggctcgtgat ttggtacgct tagtagaagt ctatgatctt cattatatcc agtcggtcga
     3781 tatgttccca catacagctc gaactgaagc tgttgtaaaa ttaataaaaa aagttt
//
LOCUS       DBDESG010000095         2836 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.095, whole genome shotgun sequence.
ACCESSION   DBDESG010000095 DBDESG010000000
VERSION     DBDESG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.095"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            398..874
                     /locus_tag="ACKB38_002110"
     CDS             398..874
                     /locus_tag="ACKB38_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGL7980803.1"
                     /translation="MVLDYFFDKNLVFCLEADSQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPITFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYTYIAEATA"
     gene            912..1217
                     /locus_tag="ACKB38_002111"
     CDS             912..1217
                     /locus_tag="ACKB38_002111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGL7980804.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            1265..2740
                     /locus_tag="ACKB38_002112"
     CDS             1265..2740
                     /locus_tag="ACKB38_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGL7980805.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNTAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          869 a          538 c          547 g          882 t
ORIGIN      
        1 ttatttacca tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg
       61 aactatccgt gagttcacta atctggagat ttttcaatag acttcgttat tgggcggtta
      121 cgatatgatc actgacttcg tcagtcttat ctacaacctc aaaacagtgt tttgagcaac
      181 ctgcgactag cttcctagtt tactctttga ttttcattga atattagaac agaaaaaatg
      241 cttggagtat ttgtttgtgt gtttattttt atataacaaa ctataaacaa aataaaaata
      301 taaaaaaaga gacaaaaaag aacagaaagt aattgacaac ggtttcatat agtgttatac
      361 ttatggcata aagttaatgg aaagaaggga aaaacttatg gtattagatt atttctttga
      421 caaaaacctt gtgttttgct tagaagcgga tagtcaagaa caactctttg atcaggttgc
      481 aactttattg gaagaacgag aaattgtcac tccaacttat cgtgaagcct tgatcacgcg
      541 tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaaggact tgccaaatgt
      601 agcgattcct cacacagata ttgttcataa tctagctgag aaagtggtgg ttgttcgatt
      661 agaaaaacca ataacttttc acaatatgat agctcctgat aaggaagtag aagtatccct
      721 actcttcttt atcattaata attcaagttc aagtcaaacg aacattcttg ctcagttaat
      781 ggacttcttc acaggaaatg gtcatcttga agacctatca aaaatttctg aaccagaagc
      841 cctttatact tacattgctg aagcaaccgc ttaatcttgt ctattaaaaa ataaaatcgg
      901 aggaaatcta aatgattaaa attcttgctg cctgcggtgc aggtgttaac tcaagtcacc
      961 aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttatgatgtt cactgtgatg
     1021 cagtcatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc tttacaccaa
     1081 tcgctgcaac agaccttggt tttgaaccag gtatcccagt tatcgaagct gggccaatct
     1141 tattccgtat cccagcaatg agcgctccag tatttgacaa tattgaagca gctattaaag
     1201 aacacggatt aagctaattc taatttgtta acattcatat aacatcaaaa agggaggaac
     1261 tgttatggat gcaatctttg acctaatcgg aaaggttttc aatcccatct tagaaatggg
     1321 tggacctgtc atcatgttaa tcattttgac agtattggct ttactttttg gagtgaaatt
     1381 ctccaaagcg cttgaaggtg gtatcaaact tgccatcgct cttacaggta tcggtgctat
     1441 catcggtatg ctaaacactg ctttctcagc atcactagca aaattcgttg aaaacactgg
     1501 tatccaattg agtattaccg acgttggttg ggcaccactt gctacaatca cttggggttc
     1561 tgcttggaca ctatacttct tgctcatcat gttgattgtc aacatagtga tgctagctat
     1621 gaagaaaaca gatacacttg atgtcgatat ctttgatatc tggcacttgt ctatcacagg
     1681 tctcttgatt aaatggtatg ctgataacaa tggtgtgagt caaggggttt cactctttat
     1741 tgctacagca gctatcgtcc ttgtcggtgt gttgaaaatt atcaactctg acttgatgaa
     1801 acctacattt gatgaccttc ttaacgcccc aagttcatca ccaatgacat caactcacat
     1861 gaactacatg atgaacccag ttatcatggt tttggataag atttttgaaa aattcttccc
     1921 aggccttgat aaatatgact ttgatgctgc taaattgaac aagaaaatcg gtttctgggg
     1981 atctaaattc ttcatcggtt tcatccttgg tatcgttatc ggtattatgg gaactccaca
     2041 tccaattgca ggtgttgcag atgcagataa atggcgtctt gttatcaaag gatggttgtc
     2101 tcttggtttg actgccggtg tatctttgga actcttctca cttatcggtt catggttcat
     2161 cgcagccgta gaaccactat cacaaggtat tacaaacgtt gctactaaac gtcttcaagg
     2221 acgtaaattc aatatcggtc ttgactggcc attcatcgct ggtcgtgctg aaatctgggc
     2281 ttgtgccaac gtacttgcac caatcatgtt gattgaagca gtgcttcttt caaaagttgg
     2341 aaatggtatc ttgccacttg caggtatcat cgctatgggt gttactccag ctctcttggt
     2401 tgtaactcgt ggtaaattgc tccgtatgat tatcttcgga acactcttgt tgccactctt
     2461 ccttctttca ggtacactta ttgcaccatt tgcaacagaa cttgctaaag gtgtaggtgc
     2521 cttcccagaa ggtgtgagcc aaactcaatt gattactcac tctactcttg aaggaccaat
     2581 cgaaaaactt cttggttgga caattggtaa cactacaact ggtgatatca aagcaatcct
     2641 tggtgcagta gtcttccttg tattctatat cggtatcttt gcttggtaca gaaaacaaat
     2701 gatcaaacgt aacgaagagt acgcagcaaa agcaaaataa tgcgctccta taaaatataa
     2761 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacacagtt
     2821 ttgaggttgt agataa
//
LOCUS       DBDESG010000096         2781 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.096, whole genome shotgun sequence.
ACCESSION   DBDESG010000096 DBDESG010000000
VERSION     DBDESG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.096"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            70..1329
                     /locus_tag="ACKB38_002113"
     CDS             70..1329
                     /locus_tag="ACKB38_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGL7980806.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1404..1539
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1578..2078
                     /gene="rplJ"
                     /locus_tag="ACKB38_002114"
     CDS             1578..2078
                     /gene="rplJ"
                     /locus_tag="ACKB38_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGL7980807.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2154..2522
                     /gene="rplL"
                     /locus_tag="ACKB38_002115"
     CDS             2154..2522
                     /gene="rplL"
                     /locus_tag="ACKB38_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGL7980808.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT          869 a          523 c          617 g          772 t
ORIGIN      
        1 acctacggca aagtgaatct gaagtggttt gaagagagta caacttgtct tttagaaaag
       61 gagcctataa tgaaagtctt tcagcatgta aatatcgtga cttgtgatca agatttccat
      121 gtttatcttg atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat
      181 aagccagcgt ttttagagca agctgagcag attatagact atcagggagc ttggattatg
      241 cctggtttgg tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat
      301 gacagcaatc tccatgaatg gctcaatgac tatatttggc cagcagaatc tgagtttact
      361 cccgacatga ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca
      421 acaaccttta acgatatgta taatcccaat ggtgtggata tccagcaaat ttatcaggtg
      481 gtgaaaactt ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa
      541 acaactgctg agactataag tagaactcga tccatcatag acgaaatctt aaaatataaa
      601 aatccaaatt tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg
      661 ctggaagcga gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg
      721 gagaccaagg aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt
      781 cttgaagaac tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac
      841 gagagagaaa ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt
      901 aacctcaaac tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca
      961 gtcggaattg cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga
     1021 aggactgcag ctcttcttca gaagatgaaa agtggggatg ccagccagtt tccaatcgaa
     1081 acagctctca aggtactgac aatcgaaggg gctaaggtcc ttggaatgga aaatcagata
     1141 ggaagtctgg aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt
     1201 catctccaac ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat
     1261 gtagatgatg tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta
     1321 gaactttaaa agaaaaatta cgaaaaattt taaaaaagtt tgcaaaaatc ttgcattctt
     1381 tttttgacta tgctatactt atatacggtt taaaaaaact gcctaagaca gtaggggagc
     1441 tcgactcata aatatcctac cgaggacaaa acgtatcatg taaaaagaag cgtattgtac
     1501 tttcgtgtct aggtttgggc gcgtttttct ttttgaaaaa ttccccaagc aaaataatta
     1561 cggaggtgaa cacactaatg agtgaagcaa ttattgctaa aaaagcggaa ctagttgacg
     1621 tagtagctga aaaaatgaaa gctgctgcat ctatcgtcgt tgtagacgct cgtggtttga
     1681 cagttgagca agatacagtt cttcgtcgtg agcttcgtgg aagcgaagtt gagtataaag
     1741 ttattaaaaa ctcaatcttg cgtcgtgcag ctgaaaaagc tggtcttgaa gatcttgcat
     1801 ctgtatttgt tggaccatct gcagtagcat tttctaatga agatgttatc gcaccagcga
     1861 aaatcttgaa cgacttttct aaaaacgctg aagcacttga aattaaaggt ggtgcaatcg
     1921 aaggcgctgt cgcatctaaa gaagagattc ttgcacttgc aactcttcca aaccgcgaag
     1981 gacttctttc tatgctcctt tctgtacttc aagcgccagt gcgcaacgtt gctcttgcag
     2041 tcaaagcggt tgcagaaagc aaagaagacg cggcttaatc ttaagctaca cagcgtagcc
     2101 tagctacgaa aaaaactatt ataaaattta aaacttattt ggaggaaata acaatggcat
     2161 tgaacattga aaacattatt gctgaaatta aagaagcttc aatccttgaa ttgaacgacc
     2221 ttgtaaaagc tatcgaagaa gaatttggtg taactgcagc tgctcctgta gctgttgctg
     2281 cagctgatgc agctgatgct ggtgctgcta aagattcatt cgacgttgaa ttgacatctg
     2341 caggcgacaa aaaagttggc gttatcaaag ttgtacgtga aatcactggt cttggtctta
     2401 aagaagctaa agaacttgtt gacggtgcac cagcacttgt taaagaaggc gttgcaactg
     2461 cagaagctga agaaatcaaa gctaaattgg aagaagctgg agcttcagtt actcttaaat
     2521 aatagagtat cgcaatttga ttgcggaatc cctatttacc agtctttgag agcgataagc
     2581 gattgataga aactgataaa ttgatttggt ttcctgccaa aaattctttg gcaggatttt
     2641 tgttttaata aactttcggg ggtagacact agattgtcta cccctttttt tgtgtttcgg
     2701 aggtaattat gcaaaaggaa ggacagtcat cacgtcaatt cgtgaagagt catctcatga
     2761 ctatcatggg agttgatatt g
//
LOCUS       DBDESG010000097         2568 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.097, whole genome shotgun sequence.
ACCESSION   DBDESG010000097 DBDESG010000000
VERSION     DBDESG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.097"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..638)
                     /locus_tag="ACKB38_002116"
                     /pseudo
     CDS             complement(1..638)
                     /locus_tag="ACKB38_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(583..765)
                     /locus_tag="ACKB38_002117"
     CDS             complement(583..765)
                     /locus_tag="ACKB38_002117"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGL7980809.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            1100..>2568
                     /gene="pcpA"
                     /locus_tag="ACKB38_002118"
     CDS             1100..>2568
                     /gene="pcpA"
                     /locus_tag="ACKB38_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGL7980810.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVQFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKG"
BASE COUNT          881 a          394 c          426 g          867 t
ORIGIN      
        1 ctgttagtgg cttgagtttg ctggatttac gtgaagtttg tgcttgagga catatcttca
       61 tgagcccttg ataaccactg tcagccaaga ttttaccagc ttgtccgata tttctgcaac
      121 tcattttgaa caacttcata tcatgacaat agttcacagt gatatccaaa gaaacaattc
      181 tcccttgact tgtgacaatc gcttgagcct tcatagcgtg aaatttcttt ttaccagaat
      241 cattcgctaa ttcttttttt agggcgattg atttttactt ccgtcgcatc aatcattacc
      301 gtgtcctcag aactaagagg agttcttgaa atcgtaacac cactttgaac aagagttact
      361 tcaacccatt ggctccgacg gattaagttg ctttcgtgaa taccaaaatc agccgcaatt
      421 tcttcataag tgcggtattc taggcttaat ttaggttttc gtccaccttt tgcgtgttta
      481 agttgataag ctgtttttaa tacagctaac atctctttaa aagtcgtgcg ctgaacacca
      541 acaagacgct taaatcgtgt atcagttaat tgtttacttg cttcataatt tcgcagggag
      601 tctattgact ctttggtagg tgtcaatgtt tttttcatct atcccgagaa ttattttccc
      661 gccatttgta tttgcaaatg ctgagtaggt ttcccagaaa gactctggaa gattgttttt
      721 agcttttttg tattctaaat caaccccttc aaattttaag tccatatttt tcctttacat
      781 ctgttttttg tggttctggt atttgttcaa gttgagtgat aatatagcga attgaatttc
      841 gagagttttt actcagttaa tttctttttt aacctacttt aatttctttt ttaacacggg
      901 ttaaaaaaga aattaaagtg ggttaatttt tcttgactta aaatttaaaa atgatatact
      961 gtgtttatgg agagatgaca atttataaaa tgtactactc tattctacta gataatgatt
     1021 gaataaaact ttttataaca acggctagca aagttataat tttatatcta ttagaaaata
     1081 aatatgtaag gaaatttaaa tgaaaaaaac tacaatatta tcattaacta cagctgcggt
     1141 tattttagca gcatatgtcc ctaatgaacc aatcctagca gatactccta gttcggaagt
     1201 aatcaaagag actaaagttg gaagtattat tcaacaaaat aatatcaaat ataaggttct
     1261 aactgtagaa ggtaacatag gaactgttca agtgggtaat ggagttactc ctgtagagtt
     1321 tgaagctggt caagatggaa aaccattcac gattcctaca aaaatcacag taggtgataa
     1381 agtatttacc gttactgaag tagctagtca agcttttagt tattatccag atgaaacagg
     1441 tagaattgtc tactatccta gctctattac tatcccatca agcataaaaa aaatacaaaa
     1501 aaaaggcttc catggaagta aagctaaaac tattattttt gacaaaggca gtcagctgga
     1561 gaaaattgaa gatagagctt ttgatttttc tgaattagaa gagattgaat tgcctgcatc
     1621 tctagaatat attggaacaa gtgcattttc ttttagtcaa aaattgaaaa agctaacctt
     1681 ttcctcaagt tcaaaattag aattaatatc acatgaggct tttgctaatt tatcaaattt
     1741 agagaaacta acattaccaa aatcggttaa aacattagga agtaatctat ttagactcac
     1801 tactagctta aaacatgttg atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg
     1861 tgttttgttt tcaaaagata aaacccaatt aatttattat ccaagtcaaa aaaatgacga
     1921 aagttataaa acgcctaagg agacaaaaga acttgcatca tattcgttta ataaaaattc
     1981 ttacttgaaa aaactcgaat tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc
     2041 agatgcgatt aaacttgaag aaattagctt accaaatagt ttagaaacta ttgaacgttt
     2101 agccttttac ggtaatttag aattaaaaga acttatatta ccagataatg ttaaaaattt
     2161 tggtaaacac gttatgaacg gtttaccaaa attaaaaagt ttaacaattg gtaataatat
     2221 caactcattg ccgtccttct tcctaagtgg cgtcttagat tcattaaagg aaattcatat
     2281 taagaataaa agtacagagt tttctgtgaa aaaagataca tttgcaattc ctgaaactgt
     2341 tcagttctat gtaacatcag aacatataaa agatgttctt aaatcaaatt tatctactag
     2401 taatgatatc attgttgaaa aagtagataa tataaaacaa gaaactgatg tagctaaacc
     2461 taaaaagaat tctaatcagg gagtagttgg ttgggttaaa gacaaaggtt tatggtatta
     2521 cttaaacgaa tcaggttcaa tggctactgg ttgggttaaa gacaaagg
//
LOCUS       DBDESG010000098         2535 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.098, whole genome shotgun sequence.
ACCESSION   DBDESG010000098 DBDESG010000000
VERSION     DBDESG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.098"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..983)
                     /locus_tag="ACKB38_002119"
     CDS             complement(<1..983)
                     /locus_tag="ACKB38_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGL7980811.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSV"
     gene            1690..>2535
                     /locus_tag="ACKB38_002120"
     CDS             1690..>2535
                     /locus_tag="ACKB38_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGL7980812.1"
                     /translation="MQNHYTTKGEHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRLEAINFRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVN
                     QALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGT"
BASE COUNT          795 a          539 c          480 g          721 t
ORIGIN      
        1 actgacagct tagttacgtc aaaatcgcaa accgaaacac cgtctattaa ctcttttgag
       61 taggcatagt agccaccgat agcattgatt tcacggcggg catactcaga tagctcaatg
      121 actttctcaa acgactcttt accacgaagg gccaagttgc gacgtgaaat atccaaacta
      181 gccatcaaca agtaagaggc agatgtagac tgggtcaggt taattatctg acgaacgtat
      241 tcagaattca tctgctcccc gataagtaaa atggagcttt gggtcaaact cccaccagac
      301 ttatgcatgg aaactgctgc catatcagcc cctgcatcca tagcagaaat tggaagttta
      361 tcagtgaaat gcaaatgcgc tccgtgggct tcatctacta aaaccatcat gccagcttca
      421 tgagccattt ctgtcaaccc ctttaggtct gaacagatgc cgtagtaagt aggattgttg
      481 attaggatag ccttagcatc tggatggtcc tttatggcct gtgctactcg gtcattttca
      541 agacctaaag cgataccaat cttaggatct acactcatct cgatatagat gggaatggca
      601 ccacatagaa ccaacgcatt gatagcagat ttatggacat ttcgtggcag aataatctta
      661 tctcctgcct tgcaggttgc cagaatcata gtctgcaccg atgaagttgt tccaccaatc
      721 attagaaagg catggctagc tccaaaagca tctgcagcca gctcctctgc atcacgaata
      781 atcgaaatag gatggcctaa attatccaaa ggtttcatcg aattgacatc aatgcctaca
      841 catttttctc ctaagagttc gacaagttct ggatttcccc gtccacgctt gtgacctgga
      901 acatcaaagg gaacaatcct tttcttgcgt aacttcacca aggcctcata aattggggct
      961 tggttttgat ctaactcttt caaaacatac tcctttcaat atttttagga ccttatgaaa
     1021 actaaggaac taaaggttga ctcatgaaaa aagagcaggt tttcacctgc tctgcaatct
     1081 tctgacgtgt ttattttggt ttctgttgag tatgtctgtt cccgatgttt cctaactctc
     1141 tagtagcaaa tattacgtac tttattgatc gtttcacaag atgacgtgtg ctttcaccac
     1201 actaaaaatt tatgaattag gacaagtgtc ctaacatcaa cttataaaag taggctttta
     1261 accctatcaa taatgtggct tttcaaccac gcttcggcat tcaaccctgc ctgtccgtag
     1321 gcatttttta ctcgggttta tatttgctag aaaacatcat ctcattttct aagctttatt
     1381 actatatcat gtttttaaga acttgtaaag tattttttga taaaaaacaa aaatataata
     1441 gattgaaact agaatagtac acctctgctt ctaaaacatt gttagaaata gattttactg
     1501 tcctgatcga tttgttctgt tcttgtttca ttttactata tgttcgtaaa tgaaagttct
     1561 ataatgaagt tagccaccca gccctcctaa aaattagatg gttcaactag ccgatagctc
     1621 ttgtaggcat aagaaaacac ctctgtgtta tacttgttgt tcaaccacaa acacaagaaa
     1681 ggcacagaga tgcaaaacca ttatactaca aaaggcgaac atttgacaat cgatagccgt
     1741 cgcttaatcg aaagatggaa aaaagaaggg aaatcaaata gagaaattgc ctctctgctt
     1801 ggaaaagttc ctcaaactat ccacactgaa atcaagcgtg ggacagtccg acaatgtctt
     1861 ggaaaagggc gcttcaaaga ggtttattct gccgactatg ctcaacagtc ttatgaaaac
     1921 aatcgcaagc gctcggtcaa gaaatcaagc ttgaccaagg aactaaagga aaagattctc
     1981 cactatcata accaaaaatt ttcgcctgaa atgatggtga tggctaaagg ggttaacgtg
     2041 ggaatttcaa ccatttacta ttggattcat catggaaaat tggggttaag caaacaggat
     2101 ctgctttacc ctagaaaagg aaaagcgctt aagaaacagg ccagcaccaa ctttaaacct
     2161 gctggtcaat ccatcgaaca gcggcttgaa gctatcaatt ttcgcttgga gaatgggcat
     2221 tatgagattg atacagttct acttacgaga gcgaaaaact actgcttgct tgtcttgacg
     2281 gatcgaaaaa gtagacatca gatcatccga ttgattccaa ataaaagtgc tgatgtggtc
     2341 aatcaggctc taaaactcat cttaaaacaa cacaagattc tttccatcac ggcagataat
     2401 ggaacggaat tcaatcgctt gtctaatgta ttttctgagg agcacattta ttatgcacat
     2461 ccctatgcct cttgggaaag gggaactaat gagaatcaca acaggctcat tcgtagatgg
     2521 ttacctaagg gaacc
//
LOCUS       DBDESG010000099         2535 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.099, whole genome shotgun sequence.
ACCESSION   DBDESG010000099 DBDESG010000000
VERSION     DBDESG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.099"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..559
                     /locus_tag="ACKB38_002121"
     CDS             <1..559
                     /locus_tag="ACKB38_002121"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7980813.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="ACKB38_002122"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="ACKB38_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGL7980814.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2023
                     /locus_tag="ACKB38_002123"
     CDS             1424..2023
                     /locus_tag="ACKB38_002123"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7980815.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSINTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQL"
     gene            complement(1977..>2535)
                     /locus_tag="ACKB38_002124"
     CDS             complement(1977..>2535)
                     /locus_tag="ACKB38_002124"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGL7980816.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNARESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIIFKVDLLFQKVSQLFE"
BASE COUNT          866 a          365 c          438 g          866 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caataaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacactg agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tgaaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgcat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DBDESG010000100         2340 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.100, whole genome shotgun sequence.
ACCESSION   DBDESG010000100 DBDESG010000000
VERSION     DBDESG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.100"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..488
                     /gene="srtC-1"
                     /locus_tag="ACKB38_002125"
     CDS             <1..488
                     /gene="srtC-1"
                     /locus_tag="ACKB38_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HGL7980817.1"
                     /translation="IGGNSTHAVITAHTGLPTAKMFTDLTKLKVGDKFYVHNIKEVMA
                     YQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYMINTHRLLVRGHRIPYVAEVEEEF
                     IAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKKKQPEKALKALKAARKEVKVEDGQ
                     Q"
     gene            475..1368
                     /gene="srtC-2"
                     /locus_tag="ACKB38_002126"
     CDS             475..1368
                     /gene="srtC-2"
                     /locus_tag="ACKB38_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HGL7980818.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            1355..2206
                     /gene="srtC-3"
                     /locus_tag="ACKB38_002127"
     CDS             1355..2206
                     /gene="srtC-3"
                     /locus_tag="ACKB38_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HGL7980819.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
BASE COUNT          653 a          421 c          600 g          666 t
ORIGIN      
        1 cgatcggagg caattcgacc catgcggtga ttacggcaca tacaggtttg ccaacagcta
       61 agatgtttac ggatttgacc aaacttaaag ttggggataa gttttatgtg cacaatatca
      121 aggaagtgat ggcctatcaa gtggatcaag taaaggtgat tgagccgacg aactttgatg
      181 atttattgat tgtaccaggt catgattatg tgaccttgct gacttgtacg ccatacatga
      241 tcaataccca tcgtctattg gttcgggggc atcggatacc gtacgtagca gaggttgagg
      301 aagaatttat tgcagcaaac aaactcagtc atctctatcg ctacctgttt tatgtggcag
      361 ttggtttgat tgtgattctt ttatggatta ttcgacgctt gcgcaagaag aaaaaacaac
      421 cggaaaaggc tttgaaggcg ctgaaagcag caaggaagga agtgaaggtg gaggatggac
      481 aacagtagac gttcacgaaa aaaaggcaca aaaaagaaga aacatccgct gatccttctt
      541 ctgattttct tagtaggatt cgccgttgcg atatatccat tggtgtctcg ttattattat
      601 cgtattgagt caaacgaggt tattaaagag tttgatgaga cggtttccca gatggataag
      661 gcagaacttg aggagcgttg gcgcttggct caagccttca atgcgacctt gaaaccatct
      721 gaaattcttg atccttttac agagcaagag aaaaagaaag gcgtctcaga atatgccaat
      781 atgctaaagg tccatgagcg gattggctat gtggaaattc ctgcgattga tcaggaaatt
      841 ccgatgtatg tcggaacgag tgaggacatt cttcagaaag gggcagggct gttagaaggg
      901 gcttcgctgc ctgttggagg tgaaaatacc catacagtga tcactgctca cagaggattg
      961 ccaacggcag aattgttcag tcaattggat aagatgaaaa aaggggatat cttttatctt
     1021 cacgttttag atcaggtgtt ggcctaccaa gtggatcaga tagtgacggt ggagccgaat
     1081 gactttgagc ctgtcttgat tcaacatggg gaagattatg cgaccttgtt gacttgtaca
     1141 ccgtatatga ttaacagtca tcgtctgttg gtacgtggga agcggattcc gtatacggca
     1201 ccaattgcag agcggaatcg agcggtgaga gagcgtgggc aattctggtt gtggttatta
     1261 ctaggagcga tggcggtcat ccttctcttg ctgtatcgcg tgtatcgtaa tcgacggatt
     1321 gtcaaaggac tagaaaagca attggagggg cgtcatgtca aggactaaac tacgagcctt
     1381 attgggatac ttgttgatgt tggtagcctg tttgattcct atttattgtt ttggacagat
     1441 ggtgttgcag tctcttggac aggtgaaagg tcatgctaca tttgtgaaat ccatgacaac
     1501 tgaaatgtac caagaacaac agaaccattc tctcgcctac aatcaacgct tggcttcgca
     1561 aaatcgcatt gtagatcctt ttttggcgga gggatatgag gtcaattacc aagtgtctga
     1621 cgaccctgat gcagtctatg gttacttgtc tattccaagt ttggaaatca tggagccggt
     1681 ttatttggga gcagattatc atcatttagg gatgggcttg gctcatgtgg atggtacacc
     1741 gctgcctctg gatggtacag ggattcgctc agtgattgct gggcaccgtg cagagccaag
     1801 ccatgtcttt ttccgccatt tggatcagct aaaagttgga gatgctcttt attatgataa
     1861 tggccaggaa attgtagaat atcagatgat ggacacagag attattttac cgtcggaatg
     1921 ggaaaaatta gaatcggtta gctctaaaaa tatcatgacc ttgataacct gcgatccgat
     1981 tcctaccttt aataaacgct tattagtgaa ttttgaacga gtcgctgttt atcaaaaatc
     2041 agatccacaa acagctgcag ttgcgagggt tgcttttacg aaagaaggac aatctgtatc
     2101 gcgtgttgca acctctcaat ggttgtaccg tgggctagtg gtactggcat ttctgggaat
     2161 cctgtttgtt ttgtggaagc tagcacgttt actacgaggg aaataaaaag aaatgaaagg
     2221 aaagctaagg ctgttccttt ttccggctct ttgtcaactg tagtgggttg aaaaaaagct
     2281 aagctcgaga aaggacaaat tttgtccttt cttttttgat attcagagcg ataaaaatcc
//
LOCUS       DBDESG010000101         2091 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.101, whole genome shotgun sequence.
ACCESSION   DBDESG010000101 DBDESG010000000
VERSION     DBDESG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.101"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..1610)
                     /gene="rexB"
                     /locus_tag="ACKB38_002128"
     CDS             complement(<1..1610)
                     /gene="rexB"
                     /locus_tag="ACKB38_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGL7980820.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLS"
     gene            complement(1810..1965)
                     /locus_tag="ACKB38_002129"
                     /pseudo
     CDS             complement(1810..1965)
                     /locus_tag="ACKB38_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT          649 a          433 c          400 g          609 t
ORIGIN      
        1 tgggacaaac tcattccaga atggagcaag gctaggaagt cttccagact aacctgtgaa
       61 ccagcaaaaa cagtcgcaaa ttgttctaaa acatggcaga aagccttcca aacttcggct
      121 tgtctttcct gttctacagc ttccaaagtg gttgtcaaat cttgtaactg cttggtcaca
      181 gctccttctt ttagaaagac actccatttt tgtaggagtt tttcagcctt ttgttttcgg
      241 ctggcaaaga gggtttcaag aggtgctaaa attctcaggc ggaggacatt caaacgctca
      301 agattaaatt ttccatggtg ggatttggtg aaggtttgct gaaaggctgg caagccattg
      361 ataccaagat agcggatata ttgctcaaaa gcatcaatat cagactgact gaggtcagta
      421 tacaaatcag ttctaagaag attaatcaaa tcctcctgac gaaaacggta acgttttaaa
      481 gctaaaatag actcgacaaa ctgagtcaag ggatgatgag ccatggcttc gcttctacca
      541 agataaaaag gaatctgata ctggtcaaaa atggttttga gagataactg gtaagaagct
      601 acatccccca agagaatacg aaaatgcttg tagctcaggt ctgagttctc atgtaatttc
      661 tgacgaatac tacgggctac tagctccaac tcctcctttt gcgtcaaaca agaccagatt
      721 tgtaaatttt cacggtcttt ctcatcgaca tccaaagcga gttctgaaaa gtcataagaa
      781 gactccaaca aacgagaggc cttgtcaaaa ctatccatct tctcatgagt ttgagaacag
      841 tcctgagcag gcgtttggta tttagaagcc agatgatgga gaaattttac gctggcttgg
      901 tagagattgc cctcgctaaa aggactggta taggctttct tactagcata agccccgata
      961 acaatctcaa cacctttgcc gtgaagtaag tccacaaccc gctcttcctc agcagaaaaa
     1021 cgagtaaagc cgtcaatgac caaggcgatt tgattaaaat cactacttac cttgtcattc
     1081 tcaatagcct caatcaaatg ggacaactga ctttcctggg ctaactgacc ttgattaaga
     1141 taggctgtta ctttctcaaa aatcaagagt aaatccgccc tcttatcctc atctgttaaa
     1201 ttctccaagt ccaaaaaact catctgagat ttggtcatct catggtaaag ctcaattaac
     1261 tgctggatca attgaggatc ctgcttaata gcgccataaa cacgcaagtc cttgggatcg
     1321 agttcggcaa ggcatttgta aaaggccaac ccaagaccga tatcatcaag agtagtttta
     1381 gctggtaaat cattcaagac cagatagcga gccatttgag caaagcgcgt gacggtaatc
     1441 gaaaaagaag cctgctggga caagtattcc agcacggcgc gttccttttc aaaagaaaga
     1501 gagttggggg caatgtagaa gacccgcttg ccagctgcaa ctagctcttc tgcctctctt
     1561 gttagaattt ctgtcaaaga agtccgaata tcagtataaa gtaatttcat ctcagcctcg
     1621 ttggaatttt tcatcaccct atattatacc atgattagcc tcgtaaatct gttaaaatat
     1681 ttaggccatc ctttcttttc ttcatcatct gctaaatctt aaatacttag ctttacttgt
     1741 attagataga ataagtctgg ctactgaaaa tcacataata aaaaagcctc ggtaacaagg
     1801 ctttgagttt tatgattgtt tcttaggtac ggaatacact tcaatgtgtt gtcccagtat
     1861 cttaatgtcg actggtagat tgtctgattt atcgccatca acatcggact ctaattcgat
     1921 atcagaagaa gttttaatat tacgtgcctt tatatattca atattcttga tagaatgatt
     1981 gaactatagt aaattgaaac tataatagta caccgtggat gctaaaatat ttctagaaat
     2041 taatttgatt tccctaatca agctattcgt atcttatttc aatctactat a
//
LOCUS       DBDESG010000102         2001 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.102, whole genome shotgun sequence.
ACCESSION   DBDESG010000102 DBDESG010000000
VERSION     DBDESG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.102"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            635..1018
                     /locus_tag="ACKB38_002130"
     CDS             635..1018
                     /locus_tag="ACKB38_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGL7980821.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1070..1759
                     /locus_tag="ACKB38_002131"
     CDS             1070..1759
                     /locus_tag="ACKB38_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGL7980822.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFSLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVLVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1801..>2001
                     /gene="blpZ"
                     /locus_tag="ACKB38_002132"
     CDS             1801..>2001
                     /gene="blpZ"
                     /locus_tag="ACKB38_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGL7980823.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWL"
BASE COUNT          545 a          308 c          373 g          775 t
ORIGIN      
        1 atgcttactt tttaaatttt ttaacaattc aagatgtttc gatgacaatt caagatttgg
       61 atgaaaaatt ttaaaaaaca atgatatact aaacttgtca aagttgcaac gggtgctgct
      121 acattatgtt aaaaatcgct cttgattgat aagctggatt tgttaggaaa ggagtaaata
      181 attatggata agaaaaagat agtttcgact ataatatgta tagtatttct tgtagtgtca
      241 gtagataatt tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg
      301 tcttgctttt cagtgctaac atacattaaa ataaaagaga tactcttaaa tataagtaaa
      361 tgatgatttg gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat
      421 gtttgtcatt tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc
      481 ttatataggt gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat
      541 tataaaaagt agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa
      601 ttgttagtga aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat
      661 tttcttgagt agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc
      721 tattgggtgg cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa
      781 taaaggggtg gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt
      841 tggaaaaacc ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct
      901 tggtttggat gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt
      961 aatgtatcta ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc
     1021 ttacagcagt agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta
     1081 tcaacttcta ttcaaaataa gtgcagtctt ctcttactta tttttcgtat ttagtctttc
     1141 tcagctgacg cttatcgtcc aaaactattg gcaattttct tctcagatag gcaatttatt
     1201 ctggattcaa aatatcttga gtttactttt tattggagtc atgattgtgg ttcttgttaa
     1261 gacaggccat ggttatctct tccgcattcc aagaaaaaaa tggctttggt attcgatttt
     1321 gacagtatta gtgctagtgt tccagatctc ttttaacgtt cagacagcta aacatgttca
     1381 gtcaactgcg gaaggttggg ctgtattgat tggttatagt gggactaact ttgcagagct
     1441 aggtatttat atagccctgt tctttctggt tccactgatg gaagaattga tttatagagg
     1501 attactgcaa catgctttct ttaagcattc gcgatttggt cttgatttgc ttcttccttc
     1561 tattttattt gctctccctc atttttcaag cctgcctagt ctgttagata tcttcgtctt
     1621 tgcaacagtt ggaatcatct ttgctggttt gacccgctat accaagagca tttatccatc
     1681 ctatgcggtg catgtgatca ataatattgt agcgaccttc ccgtttttgc tcacttttct
     1741 acatagggtc ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggaggtcatc
     1801 atgtataaac acttattttt cctagattcc aaaactttag atcggttgac accctatatt
     1861 ctagtcttgg cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg
     1921 gtggttttta atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc
     1981 tatgtggtcg gattttggtt a
//
LOCUS       DBDESG010000103         1661 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.103, whole genome shotgun sequence.
ACCESSION   DBDESG010000103 DBDESG010000000
VERSION     DBDESG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.103"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            145..1491
                     /locus_tag="ACKB38_002133"
     CDS             145..1491
                     /locus_tag="ACKB38_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGL7980824.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          488 a          359 c          327 g          487 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtgggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 gagatgaaaa aatcgtgtga cgcaccaagg gaggagtctg cccttttgag gaaatctagc
     1561 gaggaaaaac gatactggaa cagcagaaag taaaactgac ctcatgagga ggaagaaagt
     1621 ggctcatgag gtcaggggtt ttgtaagtta catctagttg a
//
LOCUS       DBDESG010000104         1658 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.104, whole genome shotgun sequence.
ACCESSION   DBDESG010000104 DBDESG010000000
VERSION     DBDESG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.104"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            237..662
                     /gene="rplK"
                     /locus_tag="ACKB38_002134"
     CDS             237..662
                     /gene="rplK"
                     /locus_tag="ACKB38_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGL7980825.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            870..1559
                     /gene="rplA"
                     /locus_tag="ACKB38_002135"
     CDS             870..1559
                     /gene="rplA"
                     /locus_tag="ACKB38_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGL7980826.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT          553 a          310 c          352 g          443 t
ORIGIN      
        1 ctcaaaacag tgttttgagc tgacttcgtc agttttatct gcaacctcaa agctgtactt
       61 tgagcagcct gcggctagct tcctagtttg ctctttgatt ttcattgagt acaaaaaata
      121 aacttttctc ttgcaattcc agataaatag tgtataatgg atgggtatgt gaaaaacata
      181 cttgtgggag gtaaaaatct ctaattaccg ccaaaaccac aaaggaggat ttaaaaatgg
      241 ctaaaaaagt cgaaaaactt gtaaaattgc aaatccctgc tggtaaagct acaccagctc
      301 caccggttgg acctgctctt ggtcaagctg gtatcaacat catgggattc acaaaagagt
      361 tcaacgctcg tacagctgac caagctggta tgatcattcc agttgttatc tcagtttacg
      421 aagataaatc atttactttc gttacgaaaa caccaccagc tgctgttctt ttgaaaaaag
      481 ctgcaggtgt tgaaaaagga tcaggtacac ctaataaaac taaagttgct acagttactc
      541 gtgcacaagt acaagaaatt gcagaaacta agatgccaga tttgaacgca gcaaacgtag
      601 agtctgcaat gcgtatgatc gaaggtactg ctcgttctat gggattcact gttgttgact
      661 aatcaataac acagtagaaa atctcacagc agtctattag ttgcttttca tactaggcaa
      721 gtgactgagg cttgtacttg ggtacagcaa gggagcttaa ggccgtagga agagaaaaat
      781 agtagactga aaacccgcaa gacttcatca tttcgagaag tgacgtggga gatgaaaatc
      841 gattgaacca cttacaagga gaatagaaaa tggctaaaaa aagcaaacaa cttcgtgctg
      901 ctcttgagaa aatcgacagc acaaaagcat acagtgtaga agaagctgta gcacttgcaa
      961 aagaaactaa ctttgcaaaa tttgatgcaa ctgtagaagt tgcttacaac ttgaacatcg
     1021 acgttaaaaa agctgaccaa caaatccgtg gagcaatggt attgccaaac ggtactggta
     1081 aaacttcacg tgttcttgtt ttcgcacgtg gtgcaaaagc tgaagaagca aaagctgctg
     1141 gtgcagactt tgttggtgaa gatgaccttg ttgctaaaat caacgacggt tggttggact
     1201 tcgacgtagt tatcgctaca cctgatatga tggctcttgt tggacgtctt ggacgtgtcc
     1261 ttggaccacg taacttgatg ccaaacccta aaactggtac tgtaacaatg gatgttgcta
     1321 aagcagttga agagtctaaa ggtggtaaaa tcacttaccg tgctgaccgt gcaggtaacg
     1381 ttcaagcaat catcggtaaa gtatcatttg aagctgaaaa attggttgaa aactttaaag
     1441 ctttcaacga aacaatccaa aaagcaaaac cagctacagc taaaggaact tacgtaacaa
     1501 acttgactat cacaactact caaggtgttg gtatcaaagt tgacgtaaac tcactttaat
     1561 cagtagttaa agtaatgtaa aaaagttgaa gacgctatgt ctcaactttt tttgatgtac
     1621 gacgggcatg ttgtatagta gatgtgtact attctagt
//
LOCUS       DBDESG010000105         1585 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.105, whole genome shotgun sequence.
ACCESSION   DBDESG010000105 DBDESG010000000
VERSION     DBDESG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.105"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..184)
                     /locus_tag="ACKB38_002136"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="ACKB38_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            936..1316
                     /locus_tag="ACKB38_002137"
     CDS             936..1316
                     /locus_tag="ACKB38_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HGL7980827.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            1318..1515
                     /locus_tag="ACKB38_002138"
     CDS             1318..1515
                     /locus_tag="ACKB38_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980828.1"
                     /translation="MKHFFAGIGEINFVSYLLYICVGIAPFFHVYIIGSEMNFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
BASE COUNT          506 a          187 c          324 g          568 t
ORIGIN      
        1 agatagcaag cctatcgtag cttttcttat tcttggcgtc ctccagtaaa gtgtagcaga
       61 tttgcttaca catgttttta tcttgattgt atagatagag tgtggaaagg tttagcagga
      121 ttgctattcg caagtcatat aaatgttgat agtttttata gacttctaag cgttgcaaga
      181 ttttctcgga ttattgacaa ataaacggat attctgatag acgattgccc tagcggctta
      241 gtgtaaactt tacaaatttg gcttgtaatt tttttataaa attcacttca gttacttgac
      301 agttattata ttttcactta ttatatagat aaaaggatgg gataggagtt taaaaatttg
      361 aaagcgctat ccataatcat aacgcataat tttctccttt tcgtgaaaaa agttaacgta
      421 attttgaaat gagcttaata agaggtatga tgtaggtaga aaataaagat aggagaaaaa
      481 gtatgaataa aaaaatattt tgtattctag tatgtattat atcattgatt agtctagcta
      541 taatctttcc atggggctgg tcgatataat gtaagggagc ttgagttgtt agtggaggat
      601 aaaatgttaa gagtaatagt agttattcaa tctttagtaa taatactgct tgctgttgct
      661 ttattattat ctcggaaaaa ctttgattcg ttaaatgttg ttcttataat gatgatcgta
      721 tcacaattgt tgttattagt atatttgata aaatcaggga atcagtgatt agctgttatt
      781 tgaattaagt aaactgttaa gcttcaagca gtaactggtt ttaaatattt aaaataagat
      841 aaaggaagga agtctaattt aggtaactaa acaaagcctg cgatcacttg ttaggaaacc
      901 atcctaacca tgcttaataa aaaaggagaa aataaatgga actcgtatta ccaaataatt
      961 atgttgctct tgagcaagaa gagatgatgt atcttgatgg gggtggtgtt ggtcgtaact
     1021 ggtggaatag tagaggtagt tttgcaacag ttctggatgt agatttggcc atctatagtg
     1081 gtggtgcaac aatttattct gcttatgcga taaaaaaagc tatctcagct aatagagggg
     1141 ctattacgag aacattacgt agtttaataa ttaaacatgt aggtagtgca gctggccatt
     1201 tagtcaatac tgcactaaac gttgcactaa ctgttactgg attttcacta ggtggagcaa
     1261 tcgcatatgg ggctgattgg gctgacggta gcttagatgg ttatattttt gcttaaaatg
     1321 aaacattttt ttgcaggaat tggtgaaata aactttgtaa gttatttatt gtatatatgt
     1381 gtaggaatag cccctttttt tcacgtatat ataataggtt ctgaaatgaa ttttgtgaaa
     1441 atagtgttat ctattttggg agttatattt gtaagtatgt taactattgc tcgaatttat
     1501 cggacttttc tataaagaat agtttggact tgaactgtat ctaaaaacag aggttggtag
     1561 attatcagcc tctttcttgt cgttg
//
LOCUS       DBDESG010000106         1576 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.106, whole genome shotgun sequence.
ACCESSION   DBDESG010000106 DBDESG010000000
VERSION     DBDESG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.106"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            145..792
                     /locus_tag="ACKB38_002139"
     CDS             145..792
                     /locus_tag="ACKB38_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HGL7980829.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            923..1336
                     /locus_tag="ACKB38_002140"
     CDS             923..1336
                     /locus_tag="ACKB38_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980830.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1355..1537
                     /locus_tag="ACKB38_002141"
                     /pseudo
     CDS             1355..1537
                     /locus_tag="ACKB38_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          432 a          314 c          277 g          553 t
ORIGIN      
        1 tattttcagg gttttatttc tatcatttta ttttactttc atttggtggg agaatatttg
       61 aaaaaaagaa aggtagttta acctatgaaa aaatcactaa aaatttttgc tacatctaaa
      121 tagtttgacc tcttcggggt tgctttggtc gttgggattg cgattgcatc tggttacctc
      181 aactcacacc tcgataaatt tgtagactgg ggatcatgga cagctcttgt accctttgga
      241 ttgatttccg taaccaacgt tgggatttcc atgttgtcca ctcgtttcac ggggaaatta
      301 agcaaatggg gaaattactt tggtattgtt aataccattt tgtccggtgc tattgattat
      361 atccttggaa ataaggcggc catcattacc tatcccgtca ccttcctcat ttataccttt
      421 gctataaaga agtgggaagc ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag
      481 gtaaaattgg cggccatcat catttccatc atcgccttcc tctttgcctt tgtgaccaac
      541 tatatcggat atgggggcaa gatgaatctc cttgcctacg taacaactat tgcctttgca
      601 ctgtccctca ttgccaatgc tttgaatgca ttaaaactaa caactcagtg gggcttttgg
      661 ttgatttaca atttcgttca gctgacaaaa gctggtattc aaggaaactt cgccaatatc
      721 ggtaaataca tcttttatat cctcaatgca atcggagctt tatttgtctg gaatgatgaa
      781 gaagttagat aatagaagga aactcaaata aagaatacta gccaacttaa gagaatctcc
      841 cttctgctat ttttatcatt ttagttgtaa ctcccattct tttggagtag aatgaagtta
      901 gataaaagag ggaggtcttg tcatgaaaaa actctataga atccatttta tagcaattgc
      961 agttattgat ttgctactat ttgcattttt tatcactaga ctcgagacat cttttgagtg
     1021 gctcttgctc tctggtttaa ttttcttcct tgctcaagga ctcctgctat ttctcttggt
     1081 cgtccgactc aaacatcaat tcgctgagat ttatcctcaa atcaataaaa agattcgctt
     1141 ctactattta ggggttctca ccattgattt tctatttttt gttctcttag ccttcattag
     1201 ttctcagcgt ttttcatctc ttatgccaat catcactgct tgccattcta ctttttatta
     1261 tatgacagct gactacctaa gagaaaacta tccagacttt tacgacaaac acatctcttt
     1321 atgggagtgt ctctaaagaa aaggaggttt tagcatgaaa aaaatcatct tcatcaaaac
     1381 cattcaactc cttgtcattg atggaatcat gctggcattt ttgacattta aaagggggct
     1441 tacttgggac tggattttga tttatagcgg ttggctcatt ttctttcatc ctgtgctatt
     1501 gacctatctt tcaaaccaac tttgtgacca ctttagttaa ctctattccc agattagacc
     1561 gagattctgg cgtttt
//
LOCUS       DBDESG010000107         1426 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.107, whole genome shotgun sequence.
ACCESSION   DBDESG010000107 DBDESG010000000
VERSION     DBDESG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.107"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(97..801)
                     /locus_tag="ACKB38_002142"
                     /pseudo
     CDS             complement(97..801)
                     /locus_tag="ACKB38_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(785..979)
                     /locus_tag="ACKB38_002143"
     CDS             complement(785..979)
                     /locus_tag="ACKB38_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980831.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1046..1396)
                     /gene="tnpB"
                     /locus_tag="ACKB38_002144"
     CDS             complement(1046..1396)
                     /gene="tnpB"
                     /locus_tag="ACKB38_002144"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGL7980832.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGKLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
BASE COUNT          403 a          304 c          273 g          446 t
ORIGIN      
        1 gcagtttacc aagcttagcg tcgccttact cctaaacctt ggactgctat cgcataggca
       61 gaactagagg actaaagtcc taagttactg ccgcaacatg tcacaatgaa catagccaga
      121 ataatctcct aggaattctt gtactactaa accactccga cgctgatcat ggtggtacag
      181 cgtgattgct tgattctcag ctttcccaga caaaaaagtc caatagtaag gcaactgact
      241 atcactctct agaacccgat aagaggtttc atccgcatga agaagagctt gttctaacaa
      301 cttttctcgt aaaagattat aaaggggctc caaatagtat tgactcgcct tgatatgcca
      361 attagcaatt tccttacgtg tgattggtaa acccatctta gcccaatctt cttcttggcg
      421 ataattgggt accttcagat taaacttctg atggatggtg tgagcgataa tagaagctga
      481 gccaaggcta tgcgccaaag gggctttagg aataggagct ttcacgattt tatcactcgg
      541 atttttatca ctgcatgctt ggcacttata agcgtgttgg atatgatcta ttcgttttaa
      601 ttgcgcagga ataaagacta attcttgtcg ttgaagggtt gctccaatct cttttagatc
      661 tccctgacaa tcagggcaaa tgctctcttc tacttgatga tgaacttctt ctgaatcaaa
      721 ttgggcaaga agagcttgac gtttcccttt agctttctta cgtttatagg tgatttcttc
      781 tctttcaact gggtaagtca gagtcttctt ccatattttg ttcctcttca aaaagactga
      841 gttgtccaga tgggcaaaca cttttctcag aggattttcc atagagcttt tgcgttagat
      901 aagccacttg ttcacgaaga agggcaagtt cattggtgag actatcaatt gtagcactct
      961 gttgttgaat gattttcaat cttcttccat gggcttcctc tccttatttc ctaactttat
     1021 tataactaaa aagaaagtcc tcatttcaat agaaatcacg actttctgat aaatttattt
     1081 ttggagtgat agaaaagccc ttcataagcc aatctacttg ttcaggtgtg agagctttga
     1141 catccttttc tgtactgggc caagtcagtt ttccgttctc aaagcgttta tatagtagcc
     1201 aaaatccttg accatcccag taaagggctt taaagcggtc tttacgtcca ccacaaaaga
     1261 gaaaaacttg accggagaaa ggatctaatt caaagtgggt tttaaccaga taagccaatg
     1321 aatcaatgcc ttgcctcata tccgttttcc cacatacgag atagacctgc cctaggctag
     1381 atagatgaat tgtcatagag caacacttta tccaaaagct gttcta
//
LOCUS       DBDESG010000108         1343 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.108, whole genome shotgun sequence.
ACCESSION   DBDESG010000108 DBDESG010000000
VERSION     DBDESG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.108"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(88..237)
                     /gene="blpO"
                     /locus_tag="ACKB38_002145"
     CDS             complement(88..237)
                     /gene="blpO"
                     /locus_tag="ACKB38_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGL7980833.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(481..684)
                     /gene="blpN"
                     /locus_tag="ACKB38_002146"
     CDS             complement(481..684)
                     /gene="blpN"
                     /locus_tag="ACKB38_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HGL7980834.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(700..954)
                     /gene="blpM"
                     /locus_tag="ACKB38_002147"
     CDS             complement(700..954)
                     /gene="blpM"
                     /locus_tag="ACKB38_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HGL7980835.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1104..>1343)
                     /locus_tag="ACKB38_002148"
     CDS             complement(1104..>1343)
                     /locus_tag="ACKB38_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGL7980836.1"
                     /translation="LMKIYPQAQTSRKSSKLKPLTVEDKACNHALSKERSKVENIFAK
                     VKTFKMFSTTYRSHRKRFGLRMNLSAGIINHELGF"
BASE COUNT          418 a          307 c          183 g          435 t
ORIGIN      
        1 ttgctgcata ggctacacct ccaagtatag ctccacctgc agcaccagtt acacctattc
       61 ctatagcaaa tggtcccaat agaaatgtca aaccgttgtt gcacacccat caattgcgcc
      121 atatgcaacc cctgctgcac aactaatttt tcttccccaa tcaatatctc caccttcaac
      181 gcaagcaagc atttcattat ccataactgc aaattgtgac atcatttttg tatccatata
      241 gtgtatcact tttcagttac ggaacaagtt taatataaaa attatcaaaa aaacataggc
      301 aataaagaga aaaattaatt tatcatagat tagaaataat atgacaaaac aattcaatga
      361 tgttaattca atagtctttt gttttttatc ggagatactt atggatagat aaataagata
      421 ggtttgaaaa gcgaagagaa taataaagaa tatagccttc ataaaattta gctttcattt
      481 ttatgatgta gcggtatagg ctaaatatcc acaaaccact gctcctccaa ttcctcctat
      541 tgcagcgccc catggtccta gaagtctccc atatttcact ccacccgctg cacaacctaa
      601 agcagcaact acagctgctc ctccggaatt acctccataa acctcactca gcattgtttc
      661 atttatatta caataagtat tcatacaagt ctccttttat taaaatccac ccgttgcccc
      721 tgttactcct gcccaaagat ccacaccaaa tttagctcct atgtatccac atgctcccat
      781 aaatggtgct ccaacaccac tcgcagcaca aatagctgtc cctagccccc agccaccaaa
      841 agcagcacca ccaccttcta agacattagt ttgccaatta ttcttgcctc cttcaatact
      901 agataacata gttatatcca tttcatgaaa ttgttccata atttttgtat ccatgacaaa
      961 tactcttttt tatttttaat ttttgtcttg ttgtaacttt gacaagttta gtatatcatc
     1021 gttttttaaa atttttcatc cagattttga atagtcatcg aaacgtcttg aattgcaaaa
     1081 attacattag acttcctgca aaactagaat cctagttcat gattgataat accagcactc
     1141 aaattcattc gtaatccgaa gcgtttacga tgacttcgat aggttgttga aaacatttta
     1201 aacgttttta ctttggcaaa gatgttctca accttgcttc tctccttaga tagcgcatgg
     1261 ttacaggctt tatcttcaac tgttagcggt ttgagtttgc tggatttacg tgaagtttgt
     1321 gcttgaggat atatcttcat gag
//
LOCUS       DBDESG010000109         1231 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.109, whole genome shotgun sequence.
ACCESSION   DBDESG010000109 DBDESG010000000
VERSION     DBDESG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.109"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..1192)
                     /gene="ltrA"
                     /locus_tag="ACKB38_002149"
                     /pseudo
     CDS             complement(1..1192)
                     /gene="ltrA"
                     /locus_tag="ACKB38_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          323 a          284 c          208 g          416 t
ORIGIN      
        1 ccaggactgg ttttgatata gcacgtttaa gtaccgactt ctgagctact aattgataat
       61 ggtcgcccca gccagatacc ttatctgcta tccatttagg aactcctaac ttaagcaatc
      121 cccataatcg tctcgatttc ttcttccatt gcttccagat aatcactcgt aggcgagtac
      181 gcaagcgctc atctatgctg gcgactatac ttttcatatt tcccaatgag caatagttta
      241 tccatcctcg aatagacaaa ttcagttgct caatacgtct tgttaggtct atactccatt
      301 tcctctgtgt tagtttcttc aatttaaact taaatctccg aacactatct tgatgtggac
      361 ggcttttcca accatctgat aatttccaga acccaaaacc tagatatttc aactctcttg
      421 gtcatgttta ctttcaaacc tagccgtttc tcaataaaac gactgactga atacatcaca
      481 cgcttagagg ctgcctcgct tccgaccgta atcacacaat catctgcgta gcgcacaaat
      541 cgaagtcccc tcttttctaa ttccttgtcc aattcattaa gcatgatatt ggataagaga
      601 ggagataaat ttcctccctg tggtgtacca actagtgttt tataacgttg accattaatg
      661 ataacacctg aatgaagata cttacgaatc aaggattccg tatctccgtc ttcgataatg
      721 ttatgtacta aggacatcaa tctatcttga ggaactgtat cgaaaaattt ctctaggtct
      781 aatccactat ccactcatag ccgtcattta agtattctaa gagcttcatg atggcttttt
      841 cacatgacct atttggtctg aaaccataac tcgtatctga gaaatggggt tcacaaatgg
      901 ggctcatgac ttggacaatg gcctgttgaa tcattctatc cataactgtt ggaattccta
      961 gttgacggat gcctccgtct ggtttaggta tctcaactct aagaactggt tgaggcttat
     1021 attttctctg ttttatcagt tccttagtca agcgccagtt ttgtctgaga taattatcca
     1081 tctcttcgat agtcattcca tcaatcccag ctgagccttt attggatttt acttgattgt
     1141 aggcttccag catattttcg cgtgataata tcttatctag cagttttgac atgtgcgtat
     1201 tcctttctta ttttggtgcc tgagggacat c
//
LOCUS       DBDESG010000110         1204 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.110, whole genome shotgun sequence.
ACCESSION   DBDESG010000110 DBDESG010000000
VERSION     DBDESG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.110"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..246)
                     /locus_tag="ACKB38_002150"
     CDS             complement(<1..246)
                     /locus_tag="ACKB38_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGL7980837.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNL"
     gene            complement(339..997)
                     /locus_tag="ACKB38_002151"
                     /pseudo
     CDS             complement(339..997)
                     /locus_tag="ACKB38_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1104..1204)
                     /locus_tag="ACKB38_002152"
                     /pseudo
     CDS             complement(1104..1204)
                     /locus_tag="ACKB38_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          338 a          267 c          220 g          379 t
ORIGIN      
        1 taagttgctt tcgtgaatac caaaaacagc cgcaatttgt tcataagttc gatattctcg
       61 cacatattga agagtggcca taagaaggtc ttctagactt aatttaggtt ttcgtccacc
      121 ttttgcgtgt ttaagttgat aagctgtttt taatacagct aacatctctt caaaagtcgt
      181 gcgttgaaca ccaacaagac gcttaaatcg tgcatcggtt agttgtttac ttgcttcatc
      241 attcatagaa ctattatacc atattttgtt tcgcaggaag tctattgaaa aatctccaga
      301 ctggagaact cacggatagt tcctaatctg gagatttctt atttgcactt ttcttgtaca
      361 actttagtcc atggtaaata agcctctaaa acctctttgt ttacgagagt ttcctcgttt
      421 ggaagacatt ctagaagata ggatagatat ttctcgctat ttaattgatg acgtttagct
      481 gtttccaaca aactcataat aatagctctt gctttagctc cttcaaaact ttgagaaaat
      541 aaccaccctc gcttttttaa actgagctta ggctaaaaga gtccactgga ctcttttact
      601 ccgtcccata accaatgatt taatggcgcg ttcagctaga ttattggaaa ggaccagatg
      661 tccgtctttc aaaatggtct taaaggtttc ttcatacttg aggctgtatt caattgccct
      721 tcctagtttt gaacctgcta aaactgactg acgccggcac taagcaaaga agtcttccat
      781 taagggctgg agatgttctt gacgtttctg tagtcgttca tcagctggca aagcctccca
      841 gtctctttcc aaggaaaata actgatcaca ataagctaaa cctttagctc ctaaggatga
      901 tttatccgct tgcttggggg ggcgcttcaa aaaacttcct tctcacatgc gcccaacatc
      961 caacaagttc agcttcttcc agttgacgat aagcagacga gcggttcgaa gtttaccaag
     1021 cttagcgtcg ccttgctcct aaaccttgga ctgctatcgc ataggcagaa ctagaggact
     1081 aaagtcctaa gttactgccg caaaatatca caatgcacat agccagaata atctcctagg
     1141 aattcttgta ctactgaacc acttcgacac tgatcatggt ggtaaagcgt aatcccttgt
     1201 ttct
//
LOCUS       DBDESG010000111         1201 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.111, whole genome shotgun sequence.
ACCESSION   DBDESG010000111 DBDESG010000000
VERSION     DBDESG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.111"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            292..>1201
                     /locus_tag="ACKB38_002153"
     CDS             292..>1201
                     /locus_tag="ACKB38_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGL7980838.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKK"
BASE COUNT          404 a          268 c          186 g          343 t
ORIGIN      
        1 gctaaaaagg ctcactattt gttataagtt catcgtactg tctacacaca aaaaggggct
       61 tcctaagcca tctctattct tatccccata tttcccagtt ttgggtaaat ctgaacagaa
      121 aaacacttct gctctctagt ttctgctagt gaacaaaagt gttttttctg gctctataat
      181 ttctgtagtg ggtaaatccc actgtggaga ttatagggct ttttgagtat caaaaaagtc
      241 ccataagact tataatgaaa agcgcctaaa caactcatta gaaagtatct tatggaacac
      301 atcaatcata ccacattact catcggaata aaagacaaaa acatcacctt aaataaagcc
      361 attcagcacg atacccatat cgaagtcttc gctacacttg attatcaccc acctaaatgt
      421 aaacactgta aaggaaaaca aatcaaatac gacttccaaa agccttctaa aatccctttt
      481 atcgagattg gtggtttccc tagcctcatt catttgaaaa agagacgatt tcaatgcaag
      541 tcctgtcgga aagtcactgt agctgaaaca actctcgttc agaaaaattg ccaaatctcg
      601 gaaatggtga gacagaaaat tgcccaactc ctactcaaca gagaggctct tacacatatc
      661 gcttctaaat tagccatctc tacctctacc tctaccgtct atcgtaagct caagcaattt
      721 catttccaag aggattacac cactttgcct gaaatcctct cctgggatga attctcctac
      781 cagaagggga aattggcttt cattgctcag gatttcaaca ctaagaaaat catgaccatt
      841 cttgataaca gacgtcaaac aaccatccga aatcatttct tcaagtactc gaaagaagct
      901 agaaaaaaag ttaaagtcgt cactgttgat atgtctggaa gttatatccc tctcattaag
      961 aaattatttc ccaatgctaa aattgttctc gatcgtttcc acattgtcca acacatgagc
     1021 agggctctta atcagactag aatcaacatc atgaagcaat ttgatgataa atctctggaa
     1081 tacagagctc ttaaatatta ctggaaattt atcctaaaag atagtcggaa actctctctt
     1141 aagcctttct atgctagaac tttcagagag accttaactc ctagggagtg tctgaagaaa
     1201 a
//
LOCUS       DBDESG010000112         1177 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.112, whole genome shotgun sequence.
ACCESSION   DBDESG010000112 DBDESG010000000
VERSION     DBDESG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.112"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            146..310
                     /locus_tag="ACKB38_002154"
     CDS             146..310
                     /locus_tag="ACKB38_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980839.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            307..711
                     /locus_tag="ACKB38_002155"
     CDS             307..711
                     /locus_tag="ACKB38_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGL7980840.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            914..>1177
                     /locus_tag="ACKB38_002156"
     CDS             914..>1177
                     /locus_tag="ACKB38_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGL7980841.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGVHESNLLRRSQW"
BASE COUNT          357 a          192 c          216 g          412 t
ORIGIN      
        1 atgcttgctt ttttaatttt tacaattcaa gaggttttga tgaccattta tgatttgagt
       61 gatccaaggt caaaataagt gctatactag cagtgtaaat gttcttgctc aactaagata
      121 aatcgcacat ttaggaggaa atcttatgaa gaaaaaaata ctgattattt tcgttctgta
      181 tctaatcatg tccatctttc tatatccgct tagggagagt gcttggtatc agctatttta
      241 taccatagcc tatgtgattg cagttatgat ctattttgct atcaataaaa agaaaggaga
      301 aaagaaatga aaacttttct tgctaaaaaa cggaacatct tccttgcgag attgttctta
      361 ggtcagttgc ccttgcttgt ttctacttat ctatttctat ctcgtcagtt tttaaatttt
      421 tccgtagttt tccaatttct tttagtagtt attaacttgg cttctatttt ggttactgtt
      481 tatctcacta gggaaatgag gataagagag tttgaagatg atgatttggt tagtcctaga
      541 accaatcaac ttatgttcat cggcttgaca ggttttatgt ctattatttg tttgtataga
      601 ggtatcacag caggagaatc ctaccaacaa ataatcgctt atattggcgc tgttctctgc
      661 ttaatcatca tgcttctgct catgtggggc ttgaagtatt ataaaaagta gagcttaatg
      721 aagtataaaa gaaaagggta agtgtttttg caatacttac ccttttataa tagattccct
      781 gcgaaacaaa atatggtata gtatagtaga ttgaaactag aatagtacgc ctctacttct
      841 aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tatcctgttc ttatttcatt
      901 ttactatagt tctatgaatt atgaagcaag taaacaacta actgatgcac gatttaagcg
      961 tcttgttggt gttcagcgca cgacttttga agagatgcta gctgtattaa aaacagctta
     1021 tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta agcctagaag accttcttat
     1081 ggccactctt caatatgtgc gagaataccg cacttatgaa gaaattgcgg ctgattttgg
     1141 tgttcacgaa agcaacttac tccgtcggag ccaatgg
//
LOCUS       DBDESG010000113         1146 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.113, whole genome shotgun sequence.
ACCESSION   DBDESG010000113 DBDESG010000000
VERSION     DBDESG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.113"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..200
                     /locus_tag="ACKB38_002157"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACKB38_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(883..>1146)
                     /locus_tag="ACKB38_002158"
     CDS             complement(883..>1146)
                     /locus_tag="ACKB38_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGL7980842.1"
                     /translation="DLCLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSG
                     MDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          395 a          192 c          163 g          396 t
ORIGIN      
        1 acatctggtc ctttccaata atctagctga acgcgccatt aaatcattgg ttatgggacg
       61 gagtaaaaga gtccagtgga ctcttttagc ctgagctcag tttaaaaaag cgagggtggt
      121 tattttctca aagttttgaa ggagctaaag caagagctat tgttatgagc ttgttggaaa
      181 cagctaaacg tcatcaatta tagtgcgttg aatctataac agtacgcatc gactgctaaa
      241 acatttctat aaatcaattt tcctttccta atcgatttgt tcatatctta tttcaatcca
      301 ttataaatag cgagaaatat ctatcctatc ttctagaatg tcttccaaac gaggaaactc
      361 tcgtaaacaa agaggtttta gaggcctatt taccgtggac taaagttgta caagaaaagt
      421 gcaaataaga aatctccaga ttaggaacta tccgtgagtt ctctagtctg gagatttttc
      481 aatagacttc gttattggac ggttacaatt tattatatga aaatcccata tcattctcca
      541 attctatatt ttacctttct aaatgtatag attaactacc taattatagc atataacgca
      601 gattcctttc aatcgtatga tttactgcat taaattaagt aaaaaaataa aggcagtccg
      661 aagactgccg atatttatct ctcatctctt taattatggt aagtaaataa ataatttccc
      721 taaagatatg gaaattatta atactataaa tacatattat aaagtttata aatactgtaa
      781 aaatcctgaa gttaattttc taataaatat caatatgtgt tagtatcttt taaattttta
      841 gacaatttac tagttctata gacatgttta acagactcta ttttacaatt caaaaatttc
      901 atctgccact tcatttaaaa attctatatc atgggaaaca ataaaaatta ttttatccat
      961 ggttttatac ttattaatca gttcagatat ttttatcata ttggaataat ccataccact
     1021 tgaaggttcg tcaaaaaaga caaatggaga attcttgcac ataacagatg ctattgcaag
     1081 cctttgcttt tgccctcctg ataaactcat cggatgcctt tcaataaatt cgtccaggca
     1141 taaatc
//
LOCUS       DBDESG010000114          964 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.114, whole genome shotgun sequence.
ACCESSION   DBDESG010000114 DBDESG010000000
VERSION     DBDESG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.114"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            81..560
                     /locus_tag="ACKB38_002159"
     CDS             81..560
                     /locus_tag="ACKB38_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGL7980843.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            654..725
                     /locus_tag="ACKB38_002160"
     tRNA            654..725
                     /locus_tag="ACKB38_002160"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Glu,seq:ttc)
BASE COUNT          370 a          133 c          224 g          237 t
ORIGIN      
        1 gagctaaagg gaacagaatg gagaaaatgg aacaaatgtg ttttctaatc tgttagactg
       61 tatctagaaa ggggaaaatt atgattaaag aattgtatga agaagtccaa gggactgtgt
      121 ataagtgtag aaatgaatat taccttcatt tatgggaatt gtcggattgg gagcaagaag
      181 gcatgctctg cttacatgaa ttgattagta gagaagaagg actggtagac gatattccac
      241 gtttaaggaa atatttcaag accaagtttc gaaatcgaat tttagactat atccgtaaac
      301 aggaaagtca gaagcgtaga tacgataaag aaccctatga agaagtgggt gagatcagtc
      361 atcgtataag tgaggggggt ctctggctag atgattatta tctctttcat gaaacactaa
      421 gagattatag aaacaaacaa agtaaagaga aacaagaaga actagaacgc gtcttaagca
      481 atgaacgatt tcgagggcgt caaagagtat taagagactt acgcattgtg tttaaggagt
      541 ttactatccg tacccactag taagtcatgc aaaaaaaatg aaaaaaatta gaaaaagtag
      601 ttgacaaagt ttgaaaaggc tgtataatag taagagttga aaataacaac tcaggtccgt
      661 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
      721 gactatggta tgttgcgtca ggaccacttg atgaaaaaaa gtttaaaaaa acttaaaaat
      781 cttcaaaaaa gtgttgacaa gcgaaagcag ttgtgatata ctaatatagt tgtcgcttga
      841 gagaagcaag tgacaaagac ctttgaaaac tgaacaagac gaaccaatgt gcagggcgct
      901 acaacgtaag ttgtagtact gaacaatgaa aaaaacaata aatctgtcag tgacagaaat
      961 gagt
//
LOCUS       DBDESG010000115          929 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.115, whole genome shotgun sequence.
ACCESSION   DBDESG010000115 DBDESG010000000
VERSION     DBDESG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.115"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..>929)
                     /locus_tag="ACKB38_002161"
     CDS             complement(<1..>929)
                     /locus_tag="ACKB38_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. The beta-hairpin of the
                     Uvr-B subunit is inserted between the strands, where it
                     probes for the presence of a lesion; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HGL7980844.1"
                     /translation="QVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYD
                     YYQPEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPK
                     EYADSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEH
                     AFRVEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELE
                     EQLAVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLL
                     DFFPDDFLIMIDESHMTIGQIKGMYNGDRSRKE"
BASE COUNT          270 a          231 c          182 g          246 t
ORIGIN      
        1 ttctttacgc gaacggtctc cattgtacat gcccttgatt tgccctatgg tcatatgact
       61 ctcgtcaatc ataatcaaga aatcatctgg gaagaagtcg agaagcgtat aaggaggctc
      121 tccttcgctc cgtccatcca tgtggcgaga ataattttca accccattgg tatagcccat
      181 ctcacgcaac atttcgatat catactctgt ccgctgtttc aaacgctggg cttcaagcag
      241 tttaccttcc ttttcaaaga cagctaattg ttcttccaac tcggcctgaa tctttgcaat
      301 ggcaacttcc atgtggtcgt cattggtcac aaagtgtgtc gctgggaaaa tcgctaaatg
      361 atccacttct cccaacacct gacctgtcag agcctcaact tcacgaatac ggtcaatttc
      421 gtctccaaaa aattctactc gaaaggcatg ttcatctcgg gaagctggga aaatctctac
      481 cacatcccca cgaacgcgaa atcttccgcg ttggaaatca atatcattac gttcaaactg
      541 aatatcgacc aagtcattca agagtttatc acgagaaatc tctagaccag gacggagact
      601 aacgacacta tcagcgtatt ccttgggcga acccaaacca tagatacaag agactgaggc
      661 cacgacaata acatcattac gctccaaaag ggctgaggta gctgagtggc gaagcttgtc
      721 aatctcgtca ttgacagaac tatccttctc aatataggta tcgctagaag ggacataggc
      781 ctctggctgg taataatcat agtaggatac gaaatactca actgcatttt cagggaaaaa
      841 ttccttaaac tccccataga gctgaccagc cagagtttta ttgtgggcaa taaccagagt
      901 tggtttattg actttagaaa tgacctgac
//
LOCUS       DBDESG010000116          781 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.116, whole genome shotgun sequence.
ACCESSION   DBDESG010000116 DBDESG010000000
VERSION     DBDESG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.116"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..>781)
                     /locus_tag="ACKB38_002162"
     CDS             complement(<1..>781)
                     /locus_tag="ACKB38_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGL7980845.1"
                     /translation="IAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEI
                     MSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLKYDRAVRCRVKIITMD
                     MFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYW
                     KLIQQDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQDLKHHYQLYQLLLFHFQNKE
                     PEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYS"
BASE COUNT          253 a          127 c          160 g          241 t
ORIGIN      
        1 tagaatagtg tagttgaagg gcgttgacga ttttctcttt gtcctttaga aaggttttaa
       61 agacagtctg aaaaagagga tgaacctgct ttagattgtc ctcaatgagt ccgaaaaatt
      121 tctccggttc cttattctga aagtgaaaca gcaagagttg atagagctga tagtgatgtt
      181 tcaagtcttg tgaatagctc aaaagcttgt ttaaaatctc tttattggtt aaatgcatac
      241 gaaaagtagg gcgataaaaa tgtttatcgc tgagtttacg actatcctgt tgtatgagct
      301 tccagtagcg cttgatagcc ttgtattcat gagactttcg atccaattga ttcatgattt
      361 gaacacgcac acgactcata gcacggctaa gatgttgtac aatgtgaaag cgatccaaca
      421 cgattttagc acatgggaaa agttgtctag ctaagtcata gtaaggacta aacatatcca
      481 tagtaataat tttgacgcga catcggacgg ctctatcata tttaagaaag tgatttcgaa
      541 tgatagcttg tgttcttccc tcaagaacag tgatgatatt gagattgtta aaatcttgcg
      601 caatgaagct catctttccc tttgtaaaag catactcatc ccaagacata atctcaggaa
      661 gacaagaaaa atcatgttta aagtgaaagt cattgagctt acgaataaca gttgaagttg
      721 agatggaaag ctgatgggca atatcagtca tagaaatctt ttcaattaac ttttgagcaa
      781 t
//
LOCUS       DBDESG010000117          769 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.117, whole genome shotgun sequence.
ACCESSION   DBDESG010000117 DBDESG010000000
VERSION     DBDESG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.117"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            20..595
                     /locus_tag="ACKB38_002163"
     CDS             20..595
                     /locus_tag="ACKB38_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980846.1"
                     /translation="MKQKQPIVSRTKQHTFEELIQDQKLERLAKLSPDLVGRYGFTAS
                     CASSFANLIKEAYGGKNLNVVYASRMLALWNIACSCYHKADGYSLADALFSDKKICLD
                     SYYYHKNTSNTITSDVIKDVYDNYNNYMVLTREATPEYIYVVQTEMPKDSDLYFYIRE
                     VLGLSFSTMHYAFLVKVLAGALARKYKPYRN"
BASE COUNT          245 a          111 c          150 g          263 t
ORIGIN      
        1 agaaaaggag aaacaaatca tgaaacaaaa acaaccgatt gtttctagaa cgaaacaaca
       61 tacatttgaa gagcttattc aagaccaaaa gttagaaaga ttggctaagt tgtcgcccga
      121 tttggttgga aggtatggtt ttactgctag ctgtgcgtct tcatttgcga acttgattaa
      181 agaagcgtat gggggtaaaa atctaaacgt agtttatgcg agtcggatgt tggctctctg
      241 gaatattgct tgcagttgtt atcataaggc tgatgggtat tctttagcag atgcgctttt
      301 tagtgataaa aaaatttgtc tagattctta ctattaccac aagaatacct ctaataccat
      361 aactagtgat gtgataaaag atgtttacga taattataat aattatatgg ttttaactcg
      421 agaagcgaca cctgaataca tttatgttgt acaaactgaa atgccaaaag attcagattt
      481 atatttttat attagagaag ttctgggatt atcgtttagt accatgcatt atgcattttt
      541 agtcaaggtt cttgcaggag cgcttgctag aaaatataag ccatatcgaa attgaattat
      601 ttaaatttat actcttcgaa aatcaaattc aaaccacgtc agcttcgcct tgctgtactc
      661 aagtgctgtc tgtggctagc ttcttagttt gctttttgat tttcattgag tattactctt
      721 atggtagtta tttatggcat aataatattg atttgggagt tatagcgaa
//
LOCUS       DBDESG010000118          701 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.118, whole genome shotgun sequence.
ACCESSION   DBDESG010000118 DBDESG010000000
VERSION     DBDESG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.118"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(113..586)
                     /gene="tnpA"
                     /locus_tag="ACKB38_002164"
     CDS             complement(113..586)
                     /gene="tnpA"
                     /locus_tag="ACKB38_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HGL7980847.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
BASE COUNT          192 a          160 c          125 g          224 t
ORIGIN      
        1 aactggtggt ttgttctgcg gctataagcc gcttctaccg gccagggcca aaggcccacc
       61 gaaatagctt cctcgcgcac cactttcccg agcaggtgct aaagcacctt agttacttcc
      121 tcttatttat ttcgccagta aacggatcta ctgactcgaa taacgtgagc tggtctgcta
      181 ctctgtcttc ttgtaattga ttctgaatat attcagctat cactttctga ttacggccta
      241 ccgtatctac ataatagcct ctacaccaaa acttgcgatt gccatatttg tattttaaat
      301 tcgcatgctt atcaaaaatc atcaaactgc tcttgccctt taaatagccc ataaaggacg
      361 aaacactaag tttcggagga atactgataa gcatgtgaat atggtctgaa caagcattcg
      421 cttcatggat tattacaccc ttacgctcac ataagtcacg tatgattctt ccgatactag
      481 ctttgtatct gccataaatg atttgacgac gatatttggg tgcaaaaaca atatgatatt
      541 tacaattcca tgtggtatgt gataaacttt gattatcctc tctcatgagg tacctcctgt
      601 atgatatgtt gtagtggcgg agaaaccact tctatcttat cattttagga ggttcttttt
      661 gttaccacgc taaaagctct atggaaccac tagcatagct a
//
LOCUS       DBDESG010000119          692 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.119, whole genome shotgun sequence.
ACCESSION   DBDESG010000119 DBDESG010000000
VERSION     DBDESG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.119"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(74..147)
                     /locus_tag="ACKB38_002165"
     tRNA            complement(74..147)
                     /locus_tag="ACKB38_002165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="ACKB38_002166"
     tRNA            complement(157..242)
                     /locus_tag="ACKB38_002166"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="ACKB38_002167"
     tRNA            complement(250..321)
                     /locus_tag="ACKB38_002167"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="ACKB38_002168"
     tRNA            complement(344..416)
                     /locus_tag="ACKB38_002168"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="ACKB38_002169"
     tRNA            complement(435..516)
                     /locus_tag="ACKB38_002169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="ACKB38_002170"
     tRNA            complement(522..594)
                     /locus_tag="ACKB38_002170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="ACKB38_002171"
     tRNA            complement(619..691)
                     /locus_tag="ACKB38_002171"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DBDESG010000120          565 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.120, whole genome shotgun sequence.
ACCESSION   DBDESG010000120 DBDESG010000000
VERSION     DBDESG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.120"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..565
                     /locus_tag="ACKB38_002172"
                     /pseudo
     CDS             1..565
                     /locus_tag="ACKB38_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          223 a           98 c          112 g          132 t
ORIGIN      
        1 agtacctaaa atgggacgta agccaaaaac gagacctgaa gagaggacag aattagaacg
       61 tcttcaagca gaaaatgagt acctgagagc ggagaatgcc atcctaaaaa agttaagaga
      121 actccgattg aaggaggaga aagaagaaag acagaaattg ttcaagaatt aatgactgaa
      181 ttttcgttag ctcttcttct aaaagtcatt aaactagctc gttcgaccta ctactatcac
      241 ttgaaacagc tagataaacc agataaggac taagagctta aagctgaaat tcaatccatt
      301 tttatcgaac acaagggaaa ttatacttat cgccgggttc atttagaact aagaaatcgt
      361 ggttatctgg taaatcataa aagaattcaa ggcttgatga aagtactcaa tttacaagct
      421 aaaacgcgac agaaacgaaa atattcttct cataaaagac gttggcaaga aggcagagaa
      481 tctcattcaa ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac
      541 agaatttgcc attccagcaa gtact
//
LOCUS       DBDESG010000121          506 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.121, whole genome shotgun sequence.
ACCESSION   DBDESG010000121 DBDESG010000000
VERSION     DBDESG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.121"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..506
                     /locus_tag="ACKB38_002173"
                     /pseudo
     CDS             1..506
                     /locus_tag="ACKB38_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          197 a           82 c          102 g          125 t
ORIGIN      
        1 caagagggag agtacctgag agcggagaat gccatcctaa aaaagttaag agaactccga
       61 ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt ttaagaatta atgactgagt
      121 tttcgttaga tcttctttta aaagtcatta aactagctcg ttcgacctac tactatcact
      181 tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt caatccattt
      241 ttatcgaaca caaaggaaat tatgcttatc gtcggattta tttagaacta agaaatcgtg
      301 gttatctggt aaatcataaa agagttcaag gcttgatgaa agtactcaat ttacaagcta
      361 aaacgcgaca gaaacgaaaa tattcttctc ataaaggaga cgttggcaag aaggcagaga
      421 atctcattca aggccaattt gaaggctcta aaacaatgga acagtgctac acagatgtga
      481 cagaatttgc cattccagta agtact
//
LOCUS       DBDESG010000122          494 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.122, whole genome shotgun sequence.
ACCESSION   DBDESG010000122 DBDESG010000000
VERSION     DBDESG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.122"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..>494
                     /locus_tag="ACKB38_002174"
     CDS             <1..>494
                     /locus_tag="ACKB38_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HGL7980848.1"
                     /translation="EDDTAALQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAP
                     APQPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAKPEKP
                     AEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWLQNNGSWYYLNANGAMATGWV
                     KDGD"
BASE COUNT          182 a          131 c          101 g           80 t
ORIGIN      
        1 ctgaagatga tactgctgct cttcaaaata aattagctac taaaaaagct gaattggaaa
       61 aaactcaaaa agaattagat gcagctctta atgagttagg ccctgatgga gatgaagaag
      121 aaactccagc gccggctcct caaccagagc aaccagctcc tgcaccaaaa ccagagcaac
      181 cagctccagc tccaaaacca gagcaaccag ctcctgcacc aaaaccagag caaccagctc
      241 cagctccaaa accagagcaa ccagctccag ctccaaaacc agagcaacca gctaagccgg
      301 agaaaccagc tgaagagcct actcaaccag aaaaaccagc cactccaaaa acaggctgga
      361 aacaagaaaa cggtatgtgg tatttctaca atactgatgg ttcaatggca ataggttggc
      421 tccaaaacaa cggttcatgg tactacctaa acgctaacgg cgctatggca acaggttggg
      481 tgaaagatgg agat
//
LOCUS       DBDESG010000123          489 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.123, whole genome shotgun sequence.
ACCESSION   DBDESG010000123 DBDESG010000000
VERSION     DBDESG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.123"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..431)
                     /locus_tag="ACKB38_002175"
                     /pseudo
     CDS             complement(1..431)
                     /locus_tag="ACKB38_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          150 a           80 c          109 g          150 t
ORIGIN      
        1 ttagaatagt gtagttgaag ggtgttgaca agcttttctt tatctttgag gaaggtttta
       61 aagacagtct gaaaaatagg atgaacctgc ttaagattgt cctcaataag tccgaaaaat
      121 ttctccggtt ccttattctg aaagtgaaac agcaagagtt gatagagctg atagtggtgt
      181 ttcaagtctt ccgaataact caaaagcttg tttaaaatct ctttattggt taagtgcata
      241 cgaaaagtag ggcgataaaa tcgcttatca ctcagtttac gactatcctg ttgtatgagc
      301 ttccagtagc gcttgatagc cttgtattca tgagactttc gatgaaactg attcatgatt
      361 tggacatgca cacgactcat agcacggcta agatgttgta caatatgaaa gcgatcaaga
      421 acgattttag cattcgggag tgaaacagtc tgggagactg tttcagcctg agcctagaaa
      481 tttgaaagc
//
LOCUS       DBDESG010000124          477 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.124, whole genome shotgun sequence.
ACCESSION   DBDESG010000124 DBDESG010000000
VERSION     DBDESG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.124"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..429)
                     /locus_tag="ACKB38_002176"
                     /pseudo
     CDS             complement(1..429)
                     /locus_tag="ACKB38_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a           80 c          105 g          148 t
ORIGIN      
        1 agaatagtgt agttgaaggg cgttgataat cttttcttta tctttgagga aggttttaaa
       61 gacagtctga aaaataggat gaacttgctt aagattgtcc tcaataagtc cgaaaaattt
      121 ctccggttcc ttattctgaa agtgaaacag caagagttga tagagctgat agtgatgttt
      181 caagtcttgt gaatagctca aaagcttgtc taaaatctct ttattggtta aatgcatacg
      241 aaaagtagga cgataaaatc gcttatcact cagtttacgg ctatcctgtt gtatgagctt
      301 ccagtagcgc ttgatagcct tgtattcatg ggattttcga tccaattggt tcataatttg
      361 aacacgcaca cgactcatag cacggctaag atgttgtaca atgtgaaagc gatccaacac
      421 gattttagca ttcgggagtg aaacagtctg ggagactgtt tcagcctgag cctagaa
//
LOCUS       DBDESG010000125          428 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.125, whole genome shotgun sequence.
ACCESSION   DBDESG010000125 DBDESG010000000
VERSION     DBDESG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.125"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..398
                     /locus_tag="ACKB38_002177"
     CDS             <1..398
                     /locus_tag="ACKB38_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980849.1"
                     /translation="SQETNRIILLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEE
                     RINNLFFENAGLVEQDATYLSNARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTR
                     TWEILGEITGDAAPDELITQLFSQFCLGK"
BASE COUNT          134 a           95 c           81 g          118 t
ORIGIN      
        1 tcagtcagga gactaatcgc attattcttc ttaacaaaac tgacctgcct gaaacgattg
       61 aaacttcgga actacctgaa gatgtcatcc gcatttcagt tcttaaaaat caaaacatcg
      121 ataaaatcga agagagaatc aacaacctct tctttgaaaa tgctggtttg gttgagcaag
      181 atgctaccta cttgtcaaac gcccgtcaca tttccttgat tgagaaggcc gttgaaagcc
      241 tacaagctgt taaccaaggt cttgaactag ggatgccagt tgacttgctt caagttgact
      301 tgacccgtac ttgggaaatt ctaggagaaa tcactggaga tgctgctcca gatgaactca
      361 tcacccaact ctttagccaa ttctgtttag gaaaataaga aaaatccatg atccttcatt
      421 cggtcatg
//
LOCUS       DBDESG010000126          414 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.126, whole genome shotgun sequence.
ACCESSION   DBDESG010000126 DBDESG010000000
VERSION     DBDESG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.126"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..414)
                     /locus_tag="ACKB38_002178"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="ACKB38_002178"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          121 a          104 c           76 g          113 t
ORIGIN      
        1 tttccaagga aaataactga tcacaataag ctaaaccttt agctcctaag gatgatttat
       61 ctgcttgctt gggggtcgct tcaaaaaatt tccttctcac atgcgcccaa catccaacaa
      121 gttcagcttc ttccagttga cgataagctt ctagcggttc gcagtttacc aagcttagcg
      181 tcgccttgct cctaaacctt ggactgctat cgcataggca gaactagagg actaaagtcc
      241 taagttactg ccgcaacata tcacaatgaa catagccaga ataatctcct aggaattctt
      301 gtactaccga accactccga ctctgatcat gatggtacag cgtgattgct tgattctcaa
      361 ctttcccaga caaaaaggtc caatagtagg tcagatggct atgactctct agaa
//
LOCUS       DBDESG010000127          396 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.127, whole genome shotgun sequence.
ACCESSION   DBDESG010000127 DBDESG010000000
VERSION     DBDESG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.127"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(1..149)
                     /locus_tag="ACKB38_002179"
                     /pseudo
     CDS             complement(1..149)
                     /locus_tag="ACKB38_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(146..396)
                     /locus_tag="ACKB38_002180"
                     /pseudo
     CDS             complement(146..396)
                     /locus_tag="ACKB38_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612110.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          123 a           61 c           75 g          137 t
ORIGIN      
        1 gaaggtttta gaggatgaac ttgattcaga ttgtcctcaa tgagtccgaa aaatttgtca
       61 ggctccttat tctgaaagtg aaaaagcaag agttgataga gattatagtg gtgtttcaag
      121 tcttctgaat agctcaaaag tttatctata gtagattgaa actagaatag tacacctctg
      181 cttctaaaac attgttagaa atcgatttga ctgtcctgaa tgatttgtcc tgttattatt
      241 tcattttact ataaatccac gtttacgaat ctctttccac acttgttcaa tggggttcat
      301 ctctggtgtg tatggaggaa taaatgcaaa accaatatta gtcggaatct ttaaggtact
      361 tgatttatgc catatagcat tgtccataac gagtaa
//
LOCUS       DBDESG010000128          391 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.128, whole genome shotgun sequence.
ACCESSION   DBDESG010000128 DBDESG010000000
VERSION     DBDESG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.128"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..>391
                     /locus_tag="ACKB38_002181"
     CDS             <1..>391
                     /locus_tag="ACKB38_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGL7980850.1"
                     /translation="HGDHYHYIPKNELSASELAAAEAYWNGKQGSRPSSSSSYNANPA
                     QPRLSENHNLTVTPTYHQNQGENISSLLRELYAKPLSERHVESDGLIFDPAQITSRTA
                     RGVAVPHGNHYHFIPYEQMSELEKRIAR"
BASE COUNT          119 a           95 c           74 g          103 t
ORIGIN      
        1 ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc gagttagctg
       61 ctgcagaagc ctattggaat gggaagcagg gatctcgtcc ttcttcaagt tctagttata
      121 atgcaaatcc agctcaacca agattgtcag agaaccacaa tctgactgtc actccaactt
      181 atcatcaaaa tcaaggggaa aacatttcaa gccttttacg tgaattgtat gctaaaccct
      241 tatcagaacg ccatgtggaa tctgatggcc ttattttcga cccagcgcaa atcacaagtc
      301 gaaccgccag aggtgtagct gtccctcatg gtaaccatta ccactttatc ccttatgaac
      361 aaatgtctga attggaaaaa cgaattgctc g
//
LOCUS       DBDESG010000129          340 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.129, whole genome shotgun sequence.
ACCESSION   DBDESG010000129 DBDESG010000000
VERSION     DBDESG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.129"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            1..340
                     /locus_tag="ACKB38_002182"
                     /pseudo
     CDS             1..340
                     /locus_tag="ACKB38_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          120 a           59 c           65 g           96 t
ORIGIN      
        1 cgctcaaagc tatgggctac actcgaaaaa aagaaccaca cctactatga acaaaaccca
       61 gaaaaagtag ccttatttct taagaatttt aatagtttaa agcacctagc acctgtttag
      121 attgatgaaa caggattcga tacttatttt tatcgagaat atggtcgctc attaaaaggt
      181 cagttaagaa gaggcaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt
      241 ctaacaaatg gtgagttaat cgctccaata acttacgaag agacgatgac gagcgacttt
      301 tttgaagctt ggtttcagaa atttctctta ccaacattaa
//
LOCUS       DBDESG010000130          316 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.130, whole genome shotgun sequence.
ACCESSION   DBDESG010000130 DBDESG010000000
VERSION     DBDESG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.130"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..>316
                     /locus_tag="ACKB38_002183"
     CDS             <1..>316
                     /locus_tag="ACKB38_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGL7980851.1"
                     /translation="NKDTHKEIIAKLDYEAPSCPECGSQMKKYDFQKPSKIPYLETTG
                     MPTRILLRKRRFKCYHCSKIMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQ
                     LS"
BASE COUNT          109 a           66 c           48 g           93 t
ORIGIN      
        1 tcaataagga tacacacaag gaaatcatcg ccaaactgga ctatgaagct ccatcttgtc
       61 ctgagtgcgg aagtcaaatg aagaaatatg actttcaaaa accttctaaa attccttatc
      121 ttgaaacaac tggtatgcct actagaattc tccttagaaa gcgtcgtttc aagtgctatc
      181 attgttctaa aattatggtt gctgaaactt ctatcgtcaa gaagaatcat caaatccctc
      241 gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa gatttctatg actgatattg
      301 cccatcagct ttccat
//
LOCUS       DBDESG010000131          314 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.131, whole genome shotgun sequence.
ACCESSION   DBDESG010000131 DBDESG010000000
VERSION     DBDESG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.131"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..>314)
                     /locus_tag="ACKB38_002184"
     CDS             complement(<1..>314)
                     /locus_tag="ACKB38_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGL7980852.1"
                     /translation="DALVDADSDALVDAEAEALVDAEAEVLVLAEADALVLAEADALV
                     DAEADALVLAEAEALVDAEADALVLAEAEALVDADSEALVLAEADALVLAEADALVDA
                     EA"
BASE COUNT           75 a           99 c           75 g           65 t
ORIGIN      
        1 tcggcttcag catcaacgag tgcgtccgct tcagcaagta caagtgcgtc agcctcagca
       61 agtaccagtg cctctgaatc cgcatcaaca agtgcctcgg cttcagcaag taccagtgca
      121 tcggcttcgg catcaacaag tgcctcagcc tcagcaagta caagtgcgtc agcttccgca
      181 tcaacgagtg cgtccgcttc agcaagtact agtgcatcag cttcagcaag tactagcacc
      241 tcagcctcag cgtcaaccag tgcttcggct tcagcgtcaa cgagtgcgtc tgagtcagca
      301 tcaacgagtg cgtc
//
LOCUS       DBDESG010000132          273 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.132, whole genome shotgun sequence.
ACCESSION   DBDESG010000132 DBDESG010000000
VERSION     DBDESG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.132"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            <1..>273
                     /locus_tag="ACKB38_002185"
     CDS             <1..>273
                     /locus_tag="ACKB38_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HGL7980853.1"
                     /translation="QRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT          124 a           36 c           46 g           67 t
ORIGIN      
        1 aacaacgaat agtagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aag
//
LOCUS       DBDESG010000133          250 bp    DNA     linear   BCT 06-DEC-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate 889
            SAMN45165439-rid24302733.denovo.133, whole genome shotgun sequence.
ACCESSION   DBDESG010000133 DBDESG010000000
VERSION     DBDESG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN45165439
            Sequence Read Archive: SRR31600644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-DEC-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002508953.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002508953.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/05/2024 03:17:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,186
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 1,992
            CDSs (with protein)               :: 1,992
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 79 of 149
            Pseudo Genes (incomplete)         :: 73 of 149
            Pseudo Genes (internal stop)      :: 53 of 149
            Pseudo Genes (multiple problems)  :: 47 of 149
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN45165439-rid24302733.denovo.133"
                     /isolate="889"
                     /isolation_source="sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="USA"
                     /collection_date="2023"
     gene            complement(<1..>250)
                     /locus_tag="ACKB38_002186"
     CDS             complement(<1..>250)
                     /locus_tag="ACKB38_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGL7980854.1"
                     /translation="ISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQD
                     FDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITM"
BASE COUNT           82 a           47 c           51 g           70 t
ORIGIN      
        1 tccatcgtaa tgattttcac ttgacaacga acggctctat cgtagcgaag aaagtgattt
       61 cggatgacag cttgtgttct gccttcaaga acagtgataa tattaagatt atcaaaatct
      121 tgcgcaatga aactcatctt tcccttagtg aaggcatact catcccaaga cataatcttt
      181 ggaagccgag aaaaatcatg ctcaaagtga aagtcattga gcttgcgaat gacagttgaa
      241 gttgaaatgg
//
